LOCUS       DAZSNM010000001        61958 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZSNM010000001 DAZSNM010000000
VERSION     DAZSNM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 61958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61958
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.001"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            324..410
                     /gene="leuL"
                     /locus_tag="ACHW42_000001"
     CDS             324..410
                     /gene="leuL"
                     /locus_tag="ACHW42_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR8942008.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            503..2074
                     /gene="leuA"
                     /locus_tag="ACHW42_000002"
     CDS             503..2074
                     /gene="leuA"
                     /locus_tag="ACHW42_000002"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR8942009.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            2074..3165
                     /gene="leuB"
                     /locus_tag="ACHW42_000003"
     CDS             2074..3165
                     /gene="leuB"
                     /locus_tag="ACHW42_000003"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR8942010.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3168..4568
                     /gene="leuC"
                     /locus_tag="ACHW42_000004"
     CDS             3168..4568
                     /gene="leuC"
                     /locus_tag="ACHW42_000004"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR8942011.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4579..5184
                     /gene="leuD"
                     /locus_tag="ACHW42_000005"
     CDS             4579..5184
                     /gene="leuD"
                     /locus_tag="ACHW42_000005"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR8942012.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(5233..6411)
                     /gene="setA"
                     /locus_tag="ACHW42_000006"
     CDS             complement(5233..6411)
                     /gene="setA"
                     /locus_tag="ACHW42_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR8942013.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(6513..6644)
                     /gene="sgrT"
                     /locus_tag="ACHW42_000007"
     CDS             complement(6513..6644)
                     /gene="sgrT"
                     /locus_tag="ACHW42_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR8942014.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6733..8388
                     /gene="sgrR"
                     /locus_tag="ACHW42_000008"
     CDS             6733..8388
                     /gene="sgrR"
                     /locus_tag="ACHW42_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR8942015.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWLEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8422..8521
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8552..9535
                     /gene="thiB"
                     /locus_tag="ACHW42_000009"
     CDS             8552..9535
                     /gene="thiB"
                     /locus_tag="ACHW42_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR8942016.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9511..11121
                     /gene="thiP"
                     /locus_tag="ACHW42_000010"
     CDS             9511..11121
                     /gene="thiP"
                     /locus_tag="ACHW42_000010"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR8942017.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11105..11803
                     /gene="thiQ"
                     /locus_tag="ACHW42_000011"
     CDS             11105..11803
                     /gene="thiQ"
                     /locus_tag="ACHW42_000011"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR8942018.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11917..12681)
                     /locus_tag="ACHW42_000012"
     CDS             complement(11917..12681)
                     /locus_tag="ACHW42_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR8942019.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12767..13645)
                     /gene="araC"
                     /locus_tag="ACHW42_000013"
     CDS             complement(12767..13645)
                     /gene="araC"
                     /locus_tag="ACHW42_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR8942020.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13984..15684
                     /gene="araB"
                     /locus_tag="ACHW42_000014"
     CDS             13984..15684
                     /gene="araB"
                     /locus_tag="ACHW42_000014"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR8942021.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15695..17197
                     /gene="araA"
                     /locus_tag="ACHW42_000015"
     CDS             15695..17197
                     /gene="araA"
                     /locus_tag="ACHW42_000015"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR8942022.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17397..18092
                     /gene="araD"
                     /locus_tag="ACHW42_000016"
     CDS             17397..18092
                     /gene="araD"
                     /locus_tag="ACHW42_000016"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR8942023.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18167..20518
                     /gene="polB"
                     /locus_tag="ACHW42_000017"
     CDS             18167..20518
                     /gene="polB"
                     /locus_tag="ACHW42_000017"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR8942024.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20683..23589
                     /gene="rapA"
                     /locus_tag="ACHW42_000018"
     CDS             20683..23589
                     /gene="rapA"
                     /locus_tag="ACHW42_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR8942025.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23601..24260
                     /gene="rluA"
                     /locus_tag="ACHW42_000019"
     CDS             23601..24260
                     /gene="rluA"
                     /locus_tag="ACHW42_000019"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR8942026.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24377..25192)
                     /gene="djlA"
                     /locus_tag="ACHW42_000020"
     CDS             complement(24377..25192)
                     /gene="djlA"
                     /locus_tag="ACHW42_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR8942027.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25447..27801
                     /gene="lptD"
                     /locus_tag="ACHW42_000021"
     CDS             25447..27801
                     /gene="lptD"
                     /locus_tag="ACHW42_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR8942028.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27854..29140
                     /gene="surA"
                     /locus_tag="ACHW42_000022"
     CDS             27854..29140
                     /gene="surA"
                     /locus_tag="ACHW42_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR8942029.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29140..30129
                     /gene="pdxA"
                     /locus_tag="ACHW42_000023"
     CDS             29140..30129
                     /gene="pdxA"
                     /locus_tag="ACHW42_000023"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR8942030.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30126..30947
                     /gene="rsmA"
                     /locus_tag="ACHW42_000024"
     CDS             30126..30947
                     /gene="rsmA"
                     /locus_tag="ACHW42_000024"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR8942031.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30950..31327
                     /gene="apaG"
                     /locus_tag="ACHW42_000025"
     CDS             30950..31327
                     /gene="apaG"
                     /locus_tag="ACHW42_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR8942032.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31334..32176
                     /gene="apaH"
                     /locus_tag="ACHW42_000026"
     CDS             31334..32176
                     /gene="apaH"
                     /locus_tag="ACHW42_000026"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR8942033.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32254..32733)
                     /gene="folA"
                     /locus_tag="ACHW42_000027"
     CDS             complement(32254..32733)
                     /gene="folA"
                     /locus_tag="ACHW42_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR8942034.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32924..34786)
                     /gene="kefC"
                     /locus_tag="ACHW42_000028"
     CDS             complement(32924..34786)
                     /gene="kefC"
                     /locus_tag="ACHW42_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR8942035.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34779..35309)
                     /gene="kefF"
                     /locus_tag="ACHW42_000029"
     CDS             complement(34779..35309)
                     /gene="kefF"
                     /locus_tag="ACHW42_000029"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR8942036.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35357..36751)
                     /locus_tag="ACHW42_000030"
     CDS             complement(35357..36751)
                     /locus_tag="ACHW42_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8942037.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(36810..37097)
                     /gene="fixX"
                     /locus_tag="ACHW42_000031"
     CDS             complement(36810..37097)
                     /gene="fixX"
                     /locus_tag="ACHW42_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR8942038.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37094..38380)
                     /locus_tag="ACHW42_000032"
     CDS             complement(37094..38380)
                     /locus_tag="ACHW42_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR8942039.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38431..39372)
                     /locus_tag="ACHW42_000033"
                     /pseudo
     CDS             complement(38431..39372)
                     /locus_tag="ACHW42_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(39387..40157)
                     /locus_tag="ACHW42_000034"
     CDS             complement(39387..40157)
                     /locus_tag="ACHW42_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR8942040.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            40513..42027
                     /gene="caiT"
                     /locus_tag="ACHW42_000035"
     CDS             40513..42027
                     /gene="caiT"
                     /locus_tag="ACHW42_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR8942041.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            42058..43200
                     /gene="caiA"
                     /locus_tag="ACHW42_000036"
     CDS             42058..43200
                     /gene="caiA"
                     /locus_tag="ACHW42_000036"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR8942042.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            43329..44546
                     /gene="caiB"
                     /locus_tag="ACHW42_000037"
     CDS             43329..44546
                     /gene="caiB"
                     /locus_tag="ACHW42_000037"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFR8942043.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            44620..46173
                     /gene="caiC"
                     /locus_tag="ACHW42_000038"
     CDS             44620..46173
                     /gene="caiC"
                     /locus_tag="ACHW42_000038"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR8942044.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            46282..47067
                     /gene="caiD"
                     /locus_tag="ACHW42_000039"
     CDS             46282..47067
                     /gene="caiD"
                     /locus_tag="ACHW42_000039"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR8942045.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            47073..47663
                     /gene="caiE"
                     /locus_tag="ACHW42_000040"
     CDS             47073..47663
                     /gene="caiE"
                     /locus_tag="ACHW42_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR8942046.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(47749..48144)
                     /gene="caiF"
                     /locus_tag="ACHW42_000041"
     CDS             complement(47749..48144)
                     /gene="caiF"
                     /locus_tag="ACHW42_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR8942047.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(48406..51627)
                     /gene="carB"
                     /locus_tag="ACHW42_000042"
     CDS             complement(48406..51627)
                     /gene="carB"
                     /locus_tag="ACHW42_000042"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR8942048.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADVTEKVISVQEMHAQIK"
     gene            complement(51645..52793)
                     /gene="carA"
                     /locus_tag="ACHW42_000043"
     CDS             complement(51645..52793)
                     /gene="carA"
                     /locus_tag="ACHW42_000043"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR8942049.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(53249..54070)
                     /gene="dapB"
                     /locus_tag="ACHW42_000044"
     CDS             complement(53249..54070)
                     /gene="dapB"
                     /locus_tag="ACHW42_000044"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR8942050.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(54237..55151)
                     /gene="rihC"
                     /locus_tag="ACHW42_000045"
     CDS             complement(54237..55151)
                     /gene="rihC"
                     /locus_tag="ACHW42_000045"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR8942051.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(55255..56205)
                     /gene="ispH"
                     /locus_tag="ACHW42_000046"
                     /gene_synonym="lytB"
     CDS             complement(55255..56205)
                     /gene="ispH"
                     /locus_tag="ACHW42_000046"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR8942052.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(56207..56656)
                     /gene="fkpB"
                     /locus_tag="ACHW42_000047"
     CDS             complement(56207..56656)
                     /gene="fkpB"
                     /locus_tag="ACHW42_000047"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR8942053.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(56743..57237)
                     /gene="lspA"
                     /locus_tag="ACHW42_000048"
     CDS             complement(56743..57237)
                     /gene="lspA"
                     /locus_tag="ACHW42_000048"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR8942054.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(57237..60053)
                     /gene="ileS"
                     /locus_tag="ACHW42_000049"
     CDS             complement(57237..60053)
                     /gene="ileS"
                     /locus_tag="ACHW42_000049"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR8942055.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(60096..61037)
                     /gene="ribF"
                     /locus_tag="ACHW42_000050"
     CDS             complement(60096..61037)
                     /gene="ribF"
                     /locus_tag="ACHW42_000050"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR8942056.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(61045..61263)
                     /locus_tag="ACHW42_000051"
     CDS             complement(61045..61263)
                     /locus_tag="ACHW42_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR8942057.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            61366..61629
                     /gene="rpsT"
                     /locus_tag="ACHW42_000052"
     CDS             61366..61629
                     /gene="rpsT"
                     /locus_tag="ACHW42_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR8942058.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        14903 a        16927 c        16112 g        14016 t
ORIGIN      
        1 attcaccaaa tcaatatcaa aaaaaatcgc aaaacatata attcaataca aatcatcagg
       61 ataggttttg caacgcgtgc attttgtccc ctttttcctc gttgattaga tgcaaaaatt
      121 tatgctgaaa tatgtcaacc gatgaaaagc gtcggtagtt aagcagaaat taatatcgct
      181 tactttaacc accgtagcac aattagctaa ttttacggat gcagaactca cgctggcggg
      241 acgtttttat tgcgtcaggg ttgacatccg tttttgtatc cagtaactct aaaagcatat
      301 cgcattcatc tggagctgat ttaatgactc acatcgttcg ctttatcggt ctactactac
      361 taaacgcatc ttctttgcgc ggtagacgag tgagcggcat ccagcattaa gccagcacgc
      421 agtcaaacaa aaaacccgcg ccattgcgcg ggttttttta tgcccgaagc gaggcgctct
      481 aaaagagaca aggacccaaa ccatgagcca gcaagtcatt attttcgata ccacattgcg
      541 cgacggtgaa caggcgttac aggcaagctt gagtgtgaaa gaaaaactgc aaattgcgct
      601 ggcccttgag cgtatgggtg ttgacgtgat ggaagtcggt ttccccgtct cttcgccggg
      661 cgattttgaa tcggtgcaaa ccatcgcccg ccaggttaaa aacagccgcg tatgtgcgtt
      721 agctcgctgc gtggaaaaag atatcgacgt ggcggccgaa tccctgaaag tcgccgaagc
      781 cttccgtatt cataccttta ttgctacctc cccaatgcac atcgccacca agctgcgcag
      841 caccctagac gaggtgatcg aacgcgctat ctatatggtg aaacgcgccc gtaattacac
      901 cgatgatgtt gaattttctt gcgaagatgc cgggcgtaca cccattgccg atctggcgcg
      961 agtggtcgaa gcggcgatta atgccggtgc caccaccatc aacattccgg acaccgtggg
     1021 ctacaccatg ccgtttgagt tcgccggaat catcagcggc ctgtatgaac gcgtacctaa
     1081 catcgacaaa gccattattt ccgtacatac ccacgacgat ttaggcctgg cggtcggcaa
     1141 ctcactggcg gcggtacatg ccggtgcacg ccaggtggaa ggtgcaatga acgggatcgg
     1201 cgagcgtgcc ggtaactgtt cgctggaaga agtcatcatg gcgattaaag ttcgtaagga
     1261 tattctcaac gtccacaccg ccattaatca ccaagagata tggcgcacca gccagttagt
     1321 tagccagatt tgtaatatgc cgatcccggc aaacaaagcc attgttggca gcggcgcatt
     1381 cgctcactcc tccggtatcc accaggatgg cgtgctgaaa aaccgcgaaa actacgaaat
     1441 catgacacca gaatctattg gtctgaacca aatccagctg aatctgacct ctcgttcggg
     1501 gcgtgcggcg gtgaaacatc gcatggatga gatggggtat aaagaaagtg aatataattt
     1561 agacaatttg tacgatgctt tcctgaagct ggcggacaaa aaaggtcagg tgtttgatta
     1621 cgatctggag gcgctggcct tcatcggtaa gcagcaagaa gagccggagc atttccgtct
     1681 ggattacttc agcgtgcagt ctggctctaa cgatatcgcc accgccgccg tcaaactggc
     1741 ctgtggcgaa gaagtcaaag cagaagccgc caacggtaac ggtccggtcg atgccgtcta
     1801 tcaggcaatt aaccgcatca ctgactataa cgtcgaactg gtgaaataca gcctgaccgc
     1861 caaaggtcac ggtaaagatg cgctgggtca ggtggatatt gtcaccaact acaacggtcg
     1921 ccgcttccac ggcgtcggcc tggccaccga tattgtcgag tcctccgcca aagccatggt
     1981 gcacgtactg aacaatatct ggcgtgccgc agaagtcgaa aaagagttgc aacgcaaaac
     2041 tcaacacaac gaaaacaaca aggaaaccgt gtgatgtcga agaattacca tattgccgta
     2101 ttgccgggag acggtattgg tccggaagtg atgacccagg cgctgaaagt gctgaatgcc
     2161 gtgcgcaacc gctttgcgat gcgcatcacc accagccatt acgatgtagg cggcgcagcc
     2221 attgataacc acgggcaacc actgccgcct gcgacggttg aaggttgtga gcaagccgat
     2281 gccgtgctgt ttggctcggt aggcggcccg aagtgggaac atttaccacc agaccagcaa
     2341 ccagaacgcg gcgcgctgct gccgctgcgt aagcacttca aattattcag caacctgcgc
     2401 ccggcaaaac tgtatcaggg gctggaagca ttctgtccgc tgcgtgcaga cattgccgct
     2461 aacggcttcg acatccagtg cgtgcgcgaa ctgaccggcg gcatctattt cggtcagcca
     2521 aaaggccgcg aaggtagcgg acaatatgaa aaagcgtttg ataccgaggt gtatcaccgt
     2581 tttgagatcg agcgtattgc ccgcatcgcg tttgaatctg ctcgtaaacg tcgccacaaa
     2641 gtcacctcaa tcgacaaagc taacgtgctg caatcctcta ttttatggcg tgaaatcgtc
     2701 aacgagatcg ccacggaata cccggatgtc gaactggcgc atatgtacat cgacaacgcc
     2761 accatgcagc tgattaaaga tccatcacag tttgacgttc tgctgtgctc caacctgttt
     2821 ggcgacattc tgtctgacga gtgcgcaatg atcactggct cgatgggcat gttgccttcc
     2881 gccagcctga acgagcaagg ttttggactg tatgaaccgg cgggcggctc ggcaccagat
     2941 atcgcaggca aaaacatcgc caacccgatt gcacaaatcc tgtcgctggc actgctgctg
     3001 cgttacagcc tggatgccga tgatgcggct tccgccattg aacgcgccat taaccgtgca
     3061 ttagaagaag gcattcgcac cggggattta gcccgtggcg ctgccgccgt tagtaccgat
     3121 gaaatgggcg atatcattgc ccgctatgtg gcagaagggg tgtaatcatg gctaaaacgt
     3181 tatacgaaaa attattcgac gctcacgtag tgtacgaagc cgaaaacgaa accccgctgt
     3241 tatatatcga ccgccacctg gtgcatgaag tgacctcacc gcaggcgttc gatggtctgc
     3301 gcgcccacgg tcgcccggta cgtcagccgg gcaaaacctt cgccactatg gatcacaacg
     3361 tctctaccca gaccaaagac attaatgcct gtggtgaaat ggcgcgcatc cagatgcagg
     3421 agctgattaa aaactgcaaa gaatttggcg tcgagctgta tgacctgaat cacccgtatc
     3481 aggggatcgt ccacgtaatg gggccggaac agggcgtcac cttgccgggg atgaccattg
     3541 tctgcggcga ctcgcatacc gccacccacg gcgcgtttgg cgcactggcc tttggtatcg
     3601 gcacttccga agttgaacac gtactggcaa cacaaaccct gaaacagggc cgtgcgaaga
     3661 ccatgaaaat tgaagtccag ggcaaagccg cgccgggcat taccgcaaaa gatatcgtgc
     3721 tggcaattat cggtaaaact ggcagcgcag gcggcaccgg gcatgtggtg gagttttgcg
     3781 gcgaagcaat tcgtgattta agcatggaag gtcgtatgac cctgtgcaat atggcgatcg
     3841 aaatgggcgc aaaagccgct ctggttgctc ctgacgaaac cacctttaac tatgtcaaag
     3901 gccgtctgca tgcgccgaaa ggcaaagatt tcgacgacgc cgttgcctac tggaaaaccc
     3961 tgcaaaccga cgaaggcgca actttcgata ccgttgtcac tctgcaagca gaagaaattt
     4021 caccgcaggt cacctggggc accaatcccg gccaggtgat ttctgtgaac gacaatattc
     4081 ccgatccggc ttcgtttgcc gatccggttg aacgtgcgtc ggcagaaaaa gcgctggcct
     4141 atatggggct gaaaccgggt attccgctga ccgaagtggc tatcgacaaa gtgtttatcg
     4201 gttcctgtac caactctcgc attgaagatt tacgcgcggc agcggagatc gccaaagggc
     4261 gaaaagtcgc gccaggcgtg caggcactgg tggttcccgg ctctggcccg gtaaaagccc
     4321 aggcggaagc ggaaggtctg gataaaatat ttattgaagc cggttttgaa tggcgcttgc
     4381 ctggctgctc aatgtgtctg gcgatgaaca acgaccgtct gaatccgggc gaacgttgtg
     4441 catccaccag caaccgtaac tttgaaggcc gccaggggcg cggcgggcgc acgcatctgg
     4501 tcagcccggc aatggctgcc gctgctgctg tgaccggaca tttcgccgac attcgcaaca
     4561 ttaaataagg agcacaccat ggcagagaaa tttatcaaac acacaggcct ggtggttccg
     4621 ctggatgccg ccaatgtcga taccgatgca atcatcccga aacagttttt gcagaaagtg
     4681 acccgtacgg gttttggcgc gcatctgttt aacgactggc gttttctcga tgaaaaaggc
     4741 caacagccaa acccggactt cgtgctgaac ttcccgcagt atcagggcgc ttccattttg
     4801 ctggcacgag aaaacttcgg ctgtggctcc tcgcgtgagc acgcgccctg ggcattgacc
     4861 gactacggtt ttaaagtggt gattgcgccg agttttgctg acatcttcta cggcaatagc
     4921 tttaacaacc agctgctgcc ggtgaaatta agcgatgcag aagtggacga actgtttgcg
     4981 ctggtgaaag ccaatccggg gatccatttc gacgtggatc tggaagcgca agaggtgaaa
     5041 gcgggagaga aaacctatcg ctttaccatc gatgccttcc gccgccactg catgatgaat
     5101 ggtctggaca gcatcggcct gaccctgcag cacgacgacg ctattgcgtc ttatgaagca
     5161 aaacaacctg cgtttatgaa ttagacccct tgcccggtca aatgaccggg ctttccgcta
     5221 tcgtccacgt catcaaacgt ctttaacctt tgcggttaaa aataatgcga caacagaaat
     5281 aatcgcaatt acccagtaga cagcaaagtg cccccaactt tgtgcaattg ctccctgaat
     5341 aacaccagcc agaattaccc cggtagagat agtgttggta aataaggtgg tagctgcccc
     5401 cgctcttcca ggcattaaat cctgaaacca tagcatccca atacccgcaa caatgccgat
     5461 aaatacagcg ttaaaaagtt gcagcgtcat caacgccata cggctatgaa agaaaatcaa
     5521 tccagtgtaa aacagcactc ctgccaccac tgcaatgacc atcattcgcc gcttaccata
     5581 acgtttgaca tagtagccag ccagaatcat tgctggtatt tccagtccag ctgccgtccc
     5641 catcaggaaa cccgccagtt tgtctggcaa tcctaaatcg ctactgatcc acaatggcat
     5701 atcaataatg tacatagtgt tgcaggtcca cattaacgtc gaggcgacaa ataacatccg
     5761 tacgttacta tcctgccagt caccttgcat tgataaagca ttttccgacg gcagttctac
     5821 ccgcgccaca gacagaagca taaatgcaat caataccaga ctgagtgtga atatcccggc
     5881 ggcaatcgaa aacatcactg taaagccgta attcaacgcc agcataaagg ccagcggtgg
     5941 accgattacc catgccagag aaagctgcgc acgcatcacc gagctaaaca tcaccacttc
     6001 tcgcgccgag ttatccgcgt attcccgcgc cagagcaaat aactgtggca ttgccgtatt
     6061 ggccagagat gccagaagca caccacaggt gataagcgtc agataatgac gattaaatgc
     6121 aaacagtagc gcattgccaa tagccatcaa acagcaaaat ataatcagtt ttcgccgatc
     6181 gccctgactg tcagaacgtt ttgccaacca gaggcttacg ccgatcccag caatagcatt
     6241 caccgtataa aagagtccga tccagaaagg ttgcgcgcca acctcacgac tcagaaataa
     6301 gctcaatgta ggagcctgta gcgccccggc cacccccatc ataaaagcga ccagcataaa
     6361 tgccgcgtaa acaccgttca tacggcgagc catcgtcatt atccagatca tacgttccct
     6421 ttttagcgcg gcgagaataa aaaaaaccag caggtataat ctgctggcgg gtgattttac
     6481 accaatactc agtcacacat gatgcaggca agtcaacttt cagaattgcg gtcatcccac
     6541 tgcatcagtc cttcaagcat ggttaatcgt tgtgggacgc ttaaccaacg caaccagcac
     6601 aacttcgctg tcgcggtaaa atagtgctga taaaactgac gcatggggca cctccttgct
     6661 tcatcgttag gaattattga cttaatatag ggaaaataaa attgctgtct tttgcgcagg
     6721 agttcccctt ttatgccatc tgctcgtctg caacaacagt tcatccgcct gtggcaatgc
     6781 tgcgagggta aatcgcagga cacaacgctc aacgaactgg cagcgttatt gagctgctcg
     6841 cgtcgtcata tgcgtaccct gctcaacacc atgcaggatc gcggctggct gacgtgggaa
     6901 gcggaagtcg ggcgcggtaa acgctcgcgt ctgacattcc tctataccgg gctggcgctt
     6961 cagcaacagc gggcggaaga cctgctggag caggatcgta tcgaccaact agtgcagttg
     7021 gttggcgaca aagcgactgt gcggcaaatg ctggtttctc atctgggccg cagcttccgc
     7081 caggggcggc acatcctgcg cgtgctctac tatcgtccgt tgcgtaatct gctacctggc
     7141 agcgcattgc gccgttccga aacccatatt gcccggcaaa tcttcagttc gctaacgcgc
     7201 ataaatgagg aaaatgggga actggaagca gacatcgccc accactggca gcaaatatca
     7261 ccgcttcact ggcgtttctt tttgcgtcca ggagtccatt ttcatcatgg tcgtgaactg
     7321 gaaatggacg acgtgatcgc ctctttaaaa cgaatcaata cgctgccgct ctattcgcat
     7381 attacggaca ttgtgtcgcc gacgccctgg acgctggata tccatctcac gcagccggac
     7441 cgctggttgc cgttactgct ggggcaagtt ccggcgatga tcctgccgtg cgaatgggaa
     7501 accctcagta actttgccag ccatcctatt ggcaccggtc cgtatgcggt gattcgcaac
     7561 agcaccaatc aactgaaaat tcaggcattc gatgacttct tcggttaccg ggcattaatc
     7621 gacgaagtta acgtctgggt tctgccggaa attgccgacg agccagccgg tggtctgatg
     7681 ctaaaaggtc cacagggcga gaaaaaagag attgaaagcc gcctggagga aggttgctac
     7741 tatttactgt tcgacagccg cacccatcgc ggggcgaatc agcaagtcag ggactgggta
     7801 agctatgtgc tttctccaac taatctggtc tatttcgctg aggaacagta ccagcaactg
     7861 tggttcccgg cttatggact gctcccccgt tggcaccatg cccgcaccat aaagagcgaa
     7921 aaaccggctg gcctggaaag cctcaccctg accttttatc aggatcacag tgagcatcgg
     7981 gtgattgccg ggatcatgca gcagattctg gcaagtcacc aggtcacgct ggaaatcaaa
     8041 gagatcagct acgatcagtg gcttgaagga gagatcgaga gcgatatctg gcttaatagc
     8101 gccaacttta cgctgccgct ggatttttcg ctgttcgcgc acttgtgcga ggtgccgctg
     8161 ctccaacact gtattccgat cgactggcaa gccgacgccg cccgctggcg caatggcgaa
     8221 atgaacctgg cgaactggtg ccagcaactg gtcgccagta aagcaatggt gccgcttatc
     8281 caccactggc tgatcattca gggacaacgc agtatgcgcg gcctgcgtat gaataccctc
     8341 ggctggttcg attttaaatc agcgtggttt gcgccaccgg atccatgatt gctggtagat
     8401 aacaaaatca ctacactaac gccgttctca acggggtgcc acgcaaacgc gtgcgctgag
     8461 aaaatacccg tcgaacctga tccggataac gccggcgaag ggatttgagg ctccttctca
     8521 agtcctttgc cactcttttt tgaggtgcaa agtgttaaaa aaatgtcttc ccctgctgtt
     8581 gctgtgcaca gcgcccgttt tcgctaaacc cgttctgact gtttatacct acgattcctt
     8641 cgccgccgac tgggggcctg gtccaaagat caaaaaagct tttgaagcca actgtaactg
     8701 cgaactgaaa ctggtggcgc tggaagatgg cgtttcgctt ctcaaccgtc tacggatgga
     8761 aggcaaaaat agtaaagccg atgtggtgct ggggctggat aacaatctgt tagacgccgc
     8821 cagtaaaacc ggactatttg ccaaaagcgg tgtggcagcg gatgccgtta acgttcccgg
     8881 cggctggaat aatgacactt tcgtcccgtt tgattacggc tatttcgcct tcgtctatga
     8941 caagaacaaa ctgaaaaacc cgccgcaaag cctgaaagaa ttggttgaga gcgatcagaa
     9001 ctggcgggtg atttatcagg atccgcgtac cagcacgccg gggctgggtc tgctgttgtg
     9061 gatgcaaaaa gtctatggcg atgacgcccc acaagcctgg caaaaactgg cgaagaaaac
     9121 cgtcacggtc accaaaggct ggagcgaagc ctacggcctg tttttaaaag gtgaaagcga
     9181 tctggtactg agttacacca cctctccggc ttatcacatt ctcgaagaga aaaaagataa
     9241 ctacgccgcc gcgaacttta gcgaaggtca ctatctgcag gtggaagtcg ccgcccgcac
     9301 cgctgccagc aagcagccgg agctggcgca aaaattcctc cagtttatgg tttctccggc
     9361 ttttcagaat gcgatcccaa ccggtaactg gatgtatccg gtggcaaatg tcacgctgcc
     9421 tgccgggttt gaacaattga ccaaaccggc caccacgctg gagttcacac cagctgaagt
     9481 ggcggcacaa cgtcaggcat ggattagcga atggcaacgc gccgtcagcc gttaattccc
     9541 ggctggttaa ttccaggtgt aagcgccgcc acgctggtgg tagcggttgc gctcgcggcg
     9601 tttctcgccc tgtggtggaa cgcgccgcag ggtaactggg tggcagtctg gcaggacagc
     9661 tacctgtggc atgtggtgcg cttctccttc tggcaggcgt ttctctcggc gctactctct
     9721 gtcgtacccg cgatattcct cgcccgcgcc ctctatcgca ggcgctttcc gggtcggctg
     9781 gcgctgttgc gtctgtgcgc aatgaccttg atcctcccgg tgctagttgc tgttttcggc
     9841 attcttagcg tctatggtcg tcagggctgg ctggcatcgc tctgccaatc gctcggtctg
     9901 gagtggacct tttcgcccta cggcctgcaa ggcattttgc tggcgcacgt attttttaat
     9961 ctgccgatgg cgagccgctt attactccag gcactggaaa acattcccgg cgaacaacgt
    10021 cagcttgccg cccagcttgg gatgcgcggc tggcattttt tccgcttcgt cgaatggccg
    10081 tggctacggc gacaaatccc gccggttgcc gcgcttatct ttatgctctg tttcgccagc
    10141 ttcgccaccg tgctatcgct gggcggcggt ccgcaggcaa ccactatcga gctggctatt
    10201 tatcaggcgc tgagttacga ctacgatcct gcccgcgcgg cgatgctggc gctgctccag
    10261 atggtgtgtt gccttgggct ggtgctgttg agtcagcgac taagtaaggc cattgcgccc
    10321 ggcaccacgc tgctgcaagg ctggcgcgat ccggacgatc gtctgcatag ccgcatttgc
    10381 gacacggtgt taatagtgct ggcgctgctg ctgttgctgc caccgttgct ggcggtgatc
    10441 gtcgatgggg taaatcgcca gttgccggaa gtgctggcac aaccggtgct gtggcaggcg
    10501 ctgtggacct cgttgcgtat tgcgctggcg gcaggtgtat tgtgcgtagt gctgaccatg
    10561 atgctgctat ggagcagtcg cgaactgcgg gcgcggcaga aaatgctggc gggtcaggcg
    10621 ctggagatga gcggcatgtt gatcctcgcc atgccgggga ttgtgctggc taccggcttc
    10681 tttttactgc tcaacaacac tatcggcctg ccacaatctg ctgacggcat tgtgattttc
    10741 accaatgcgt taatggcgat cccttatgcg ctgaaagtac tggaaaaccc gatgcgcgat
    10801 atcaccgccc gctacagtat gttgtgtcag tcgctgggca ttgaaggctg gtcgcgctta
    10861 aaagtggtcg agctgcgcgc cctgaaacgt ccactggcgc aggcgctggc ttttgcctgc
    10921 gtgctgtcga ttggtgattt tggcgtagtg gcgttgttcg gtaacgatga tttccgtacc
    10981 ctgccgtttt atctctacca gcaaattggc tcctatcgca gccaggacgg cgcggtcacc
    11041 gcgttaattc tgctactact ctgttttctg ctgtttaccg tgattgaaaa actaccgggg
    11101 cgaaatgtta aaactgactg atatcacctg gctttatcac catttgccga tgcgttttag
    11161 cttaacggta gaacgcggcg agcaggtggc aatcctcggg ccaagcggcg cgggtaaaag
    11221 taccctgctg aatttgattg ccggttttct gacgccagcc agcggttcgc tgactatcga
    11281 tggtgttgat cacaccacga cgccgccgtc acgccgtccg gtgtcgatgc tgtttcagga
    11341 gaacaacctg ttcagccatc tgacggtcgc gcagaacatc gggctggggc taaatccggg
    11401 gttgaaactg aacgcggcac agcaggagaa aatgcacgcg attgcccgtc agatggggat
    11461 tgataattta atggcgcggt taccgggcga gctttccggc ggtcagcgac agcgcgtggc
    11521 gttagcgcgt tgtctggtgc gcgaacagcc gattttattg ctcgatgaac cgttctctgc
    11581 gctcgatccg gcgttacgtc aggagatgct gacgctggtg agcacgagct gccagcagca
    11641 aaaaatgacg ctattgatgg tgtcgcacag tgtggaagat gcggcgcgga tcgccacacg
    11701 ctcggtggtg gtcgccgacg ggcgcatcgc ctggcagggt aagactgaag aattgttgag
    11761 cggtaaggcg agtgcttcgg caatattggg gattacgggt tagtatgcac tgtcagatgc
    11821 ggcgtgaaca ccttatccga cctacggaca gtgcgttttg taggcctgat aagacgcggt
    11881 cagcgtcgca tgaggcaacc cgcacaagac aatcccctaa accccaacca ctttacgcaa
    11941 aatatcgata tacaccggca tcaacgggtg gcgaattaac accaccagcg ccaccacgcc
    12001 gatggcagaa atcaacggcg tcagccacaa caaacggccg cgcgacaaat aatgacttaa
    12061 acgatcggtt gctttaccgc tgcgccataa ccgccagcac agccagccac caacccacaa
    12121 aaataccgcc gtcgccagca gcaaccattt aaactcgccg ctctgcattc cggctggaat
    12181 atcgatcgcc gcgcccgcca gaatcccagg caggaagtaa aacggtggcc acagcaggca
    12241 gccgataata ttcggcgtaa tgaattttgc caccggcaga tccagcattc ccgccaccat
    12301 tggcaccagc ggacgcgtcg ggccaacaaa gcgaccgacc agaatggtga acatgctgtg
    12361 ttgatgcaac gcatgttcag ttttatcgag taatgctttg tttttcttca gaaatgacca
    12421 gcgatgcaac ggctttttaa aacgccagcc cagccagaag gagatccagt cgcccagcag
    12481 acaaccgaca atccctgcca gccaagcgtg ccagaaactt aactcgccgc tgccaatcag
    12541 cgctcccagc cccgccatca gcaccgtacc gggtagaatc aaaccgacca gcgccagcga
    12601 ctccagaaag gccaccaaca ccaccgccat caatgaatac acggtagatt gggtaataaa
    12661 gtgttccagc aatgcttgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa
    12721 ccctatgcta ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga
    12781 cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg
    12841 tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga
    12901 cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt
    12961 cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg
    13021 acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt
    13081 gatcgctgat gtactgacaa gcctcgcgta cacgattatc catcggtgga tggagcgact
    13141 cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagattcatc gccagcagct
    13201 ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat
    13261 acggctggtg cgcttcatcc gggcgaaaga aacccgtatt ggcaaatatt gacggccagt
    13321 taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc
    13381 gagcctccgg atgacaaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac
    13441 ccggtcggca gacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga
    13501 gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt
    13561 tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca
    13621 ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca
    13681 attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa
    13741 cccaaccggt aaccccgctt attaaaagca ttctgtaaca aagcaggacc aaagccatga
    13801 caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc
    13861 acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg gatcctacct
    13921 gacgcttttt atcgcaactc tctactgttt ctccataccc gtttttttgg atggagtgaa
    13981 acgatggcga ttgcaattgg cctcgatttt ggcagtgatt ctgtgcgagc tttggcggtg
    14041 gactgcgcca gcggtgaaga gatcgccacc agcgtagagt ggtatccccg ttggcaaaaa
    14101 gggcaatttt gtgatgcccc gaataaccag ttccgtcatc atccgcgtga ctacattgag
    14161 tcaatggaag cggcactgaa aaccgtgctt gcagagctta gcgtcgaaca gcgcgcagct
    14221 gtggtcggga ttggcgttga cagtaccggc tcgacgcccg caccgattga tgccgacggt
    14281 aacgtgctgg cgctgcgccc ggagtttgcc gaaaacccga acgcgatgtt cgtattgtgg
    14341 aaagaccaca ctgcggttga agaagcggaa gagattaccc gtttgtgcca cgcgccgggc
    14401 aacgttgact actcccgcta tattggcggt atttattcca gcgaatggtt ctgggcaaaa
    14461 atcctgcatg tgactcgcca ggacagcgcc gtggcgcaat ctgccgcatc gtggattgag
    14521 ctgtgcgact gggtgccagc tctgctttcc ggtaccaccc gcccgcagga tattcgtcgc
    14581 ggacgttgca gcgccgggca taaatctctg tggcacgaaa gctggggcgg cttgccgcca
    14641 gccagtttct ttgatgagct ggacccgatc ctcaatcgcc atttgccttc cccgctgttc
    14701 actgacacct ggactgccga tattccggtg ggcaccttat gcccggaatg ggcgcagcgt
    14761 ctcggcctgc ctgaaagcgt ggtgatttcc ggcggcgcgt ttgactgcca tatgggcgca
    14821 gttggcgcag gcgcacagcc taacgcactg gtaaaagtta tcggtacttc cacctgcgac
    14881 attctgattg ccgacaaaca gagcgttggc gagcgggcag ttaaaggtat ttgcggtcag
    14941 gttgatggca gcgtggtgcc tggatttatc ggtctggaag caggccaatc ggcgtttggt
    15001 gatatctacg cctggtttgg tcgcgtactc ggctggccgc tggaacagct tgccgcccag
    15061 catccggaac tgaaagcgca aatcaacgcc agccagaaac aactgcttcc ggcgctgacc
    15121 gaagcatggg ccaaaaatcc gtctctggat cacctgccgg tggtgctcga ctggtttaac
    15181 ggtcgtcgca cgccaaacgc taaccaacgc ctgaaagggg tgattaccga tcttaacctc
    15241 gctaccgaca ctccgctgct gttcggcggt ttgattgctg ccaccgcctt tggcgcacgc
    15301 gcaatcatgg agtgctttac cgatcagggg atcgccgtca ataacgtgat ggcgctgggc
    15361 ggcatcgcgc ggaaaaacca ggtcattatg caggcctgct gcgacgtgct gaatcgcccg
    15421 ctgcaaattg ttgcctctga ccagtgctgt gcgctcggtg cggcgatttt tgctgccgtc
    15481 gccgcgaaag tgcacgcaga catcccatca gcccagcaaa aaatggccag tgcggtagag
    15541 aaaaccctgc aaccgcgcag cgaacaggca caacgctttg aacagcttta tcgccgctat
    15601 cagcaatggg cgatgagcgc cgaacaacac tatcttccaa cttccgcccc ggcacaggct
    15661 gcccaggccg ttgcgactct ataaggacac gataatggcg atttttgata attatgaagt
    15721 gtggtttgtc attggcagcc agcatctgta tggcccggaa accctgcgtc aggtcaccca
    15781 acatgccgag cacgttgtta atgcgctgaa tacggaagcg aaactgccct acaaactggt
    15841 gttgaaaccg ctgggcacca cgccggatga aatcaccgct atttgccgcg acgcgaatta
    15901 cgacgatcgt tgcgctggtc tggtggtgtg gctgcacacc ttctccccgg ccaaaatgtg
    15961 gatcaacggc ctgaccatgc tcaacaaacc gttgctgcaa ttccacaccc agttcaacgc
    16021 ggcgctgccg tgggacagta tcgatatgga ctttatgaac ctgaaccaga ctgcacatgg
    16081 cggtcgcgag ttcggcttca ttggcgcgcg tatgcgtcag caacatgccg tcgttaccgg
    16141 tcactggcag gataaacaag cccatgagcg tatcggctcc tggatgcgtc aggcggtctc
    16201 taaacaggat acccgtcatc tgaaagtctg ccgatttggc gataacatgc gtgaagtggc
    16261 ggtcaccgat ggcgataaag ttgccgcaca gatcaagttc ggtttctccg tcaatacctg
    16321 ggcggttggc tatctggtgc aggtggtgaa ctccatcagc gacggcgatg ttaacgcgct
    16381 ggtcgatgag tacgaaagct gctacaccat gacgcctgcc acacaaatcc acggcgaaaa
    16441 acgacagaac gtgctggaag cggcgcgtat tgagctgggg atgaagcgtt tcctggaaca
    16501 aggtggcttc cacgcgttca ccaccacctt tgaagatttg cacggtctga aacagcttcc
    16561 aggtctgccc gtacagcgtc tgatgcagca gggttacggc tttgcgggcg aaggcgactg
    16621 gaaaaccgcc gccctgcttc gcatcatgaa ggtgatgtca accggtctgc agggcggcac
    16681 ctcctttatg gaggactaca cctatcactt cgagaaaggt aatgacctgg tgctcggctc
    16741 ccatatgctg gaagtctgcc cgtcgattgc cgtagaagag aaaccgatcc tcgacgttca
    16801 gcatctcggt attggtggta aggacgatcc tgcccgactg atcttcaata cccaaaccgg
    16861 tccagcgatt gtcgccagcc tgattgatct cggcgatcgt taccgtctgc tggttaactg
    16921 tatcgacacg gtgaaaacac cgcactccct gccgaaactg ccggtggcga atgcactgtg
    16981 gaaagcgcaa ccggatctgc caactgcttc cgaagcgtgg atcctcgctg gtggcgcgca
    17041 ccataccgtc ttcagccatg cgctgaacct caacgatatg cgccagttcg ccgagatgca
    17101 cgacattgaa atcacggtga ttgataacga cacccgcctg ccagcgttta aagacgcact
    17161 gcgctggaac gaagtgtatt acggatttcg tcgctaagta gccacatcag gtatgtaacg
    17221 cctgatgcga cgctgacgcg tcttatctgg cctacacgct gcgattttgt aggccggata
    17281 agcaaagcgc atccggcatt caacgcctga tgcgacgctg gcgcgtctta tcaggcctac
    17341 gcgctgcgat tttgtagacc ggataagcga agcgcatccg gcacgaagga gtcaacatgt
    17401 taaaagatct caaacgccag gtattagaag ccaacctggc gctgccaaaa cacaacctgg
    17461 tcacgctcac atggggcaac gtcagcgccg ttgatcgcga gcgcggcgtc tttgtgatca
    17521 aaccttccgg cgtcgattac agcgtcatga ccgctgacga tatggtcgtg gttagcatcg
    17581 aaaccggtga agtggttgaa ggtacgaaaa agccctcctc cgacacgcca actcaccggc
    17641 tgctctatca ggcattcccc tccattggcg gcattgtgca tacgcactcg cgccacgcca
    17701 ccatctgggc gcaggcgggt cagtcgattc cagcaaccgg caccacccac gccgactatt
    17761 tctacggcac cattccctgc acccgcaaaa tgaccgacgc agaaatcaac ggcgaatatg
    17821 agtgggaaac cggtaacgtc atcgtagaaa cctttgaaaa acagggtatc gatgcagcgc
    17881 aaatgcccgg cgttctggtc cattcccacg gcccgtttgc atggggcaaa aatgccgaag
    17941 atgcggtgca taacgccatc gtgctggaag aggtcgctta tatggggata ttctgccgtc
    18001 agttagcgcc gcagttaccg gatatgcagc aaacgctgct ggataaacac tatctgcgta
    18061 agcatggcgc gaaggcatat tacgggcagt aatgactgta taaaaccaca gccaatcaaa
    18121 cgaaaccagg ctatactcaa gcctggtttt ttgatggatt ttcagcgtgg cgcaggcagg
    18181 ttttatctta acccgacact ggcgggacac cccgcaaggg acagaagtct ccttctggct
    18241 ggcgacggac aacgggccgt tgcaggttac gcttgcaccg caagagtccg tggcgtttat
    18301 tcacgccgat caggttcccc gcgctcagca tattttgcag ggtgaacaag gctttcgtct
    18361 gacaccgctg gcgttaaagg attttcaccg ccagccggtg tatggccttt actgtcgcgc
    18421 ccatcgccaa ttgatgaatt acgaaaagcg tctgcgtgaa ggtggcgtta ccgtctacga
    18481 ggccgatgtg cgcccgccag aacgctatct gatggagcgg tttatcacct caccggtgtg
    18541 ggtcgagggt gatatgcaca atggcactat cgttaatgcc cgtctgaaac cgcatcccga
    18601 ctatcgtccg ccgctcaagt gggtttctat agatattgaa accacccgcc acggtgagct
    18661 gtactgcatc ggcctggaag gctgcgggca gcgcatcgtt tatatgctgg ggccggagaa
    18721 tggcgacgcc tcctcgcttg atttcgaact ggaatacgtc gccagccgcc cgctgctgct
    18781 ggaaaaactc aacgcctggt ttgcaaacca cgatcccgat gtgatcatcg gctggaacgt
    18841 ggtgcagttc gatctgcgaa tgctgcaaaa acatgccgag cgttaccgtc ttccgctgcg
    18901 tcttgggcgc gataatagcg agctggagtg gcgcgagcac ggctttaaaa acggcgtctt
    18961 ttttgcccag gctaaaggtc ggctaattat cgacggtatc gaggcgctga aatccgcgtt
    19021 ctggaatttc tcttcattct cgctggaaac tgtcgctcag gagctattag gcgaaggaaa
    19081 atctatcgat aacccgtggg atcgaatgga cgaaattgac cgccgtttcg ccgaagataa
    19141 acctgcgctg gcaacttata acctgaaaga ttgcgagctg gtgacgcaga tcttccacaa
    19201 aactgaaatc atgccatttt tactcgaacg agcaacggtg aacggcctgc cggtggatcg
    19261 gcacggcggt tcggtggcag cgtttggtca tctctatttt ccgcgaatgc atcgcgctgg
    19321 ttatgtcgcg cctaatctcg gcgaagtgcc gccgcacgcc agccctggcg gctacgtgat
    19381 ggattcacgg ccagggcttt atgattcagt gctggtgctg gactataaaa gcctgtaccc
    19441 gtcgatcatc cgcacctttc tgattgatcc cgtcgggctg gttgaaggca tggcgcaacc
    19501 tgatccagag cacagtaccg aaggttttct cgatgcctgg ttctcacggg agaaacattg
    19561 cctgccggag attgtgacta acatctggca cgggcgcgat aaagccaaac gtcagggtaa
    19621 taagccgctt tcgcaggcgc tgaagatcat tatgaatgcc ttttatggcg tgctcggcac
    19681 caccgcctgc cgcttcttcg atccgcgcct ggcatcgtcg atcaccatgc gtggtcatca
    19741 gatcatgcgg cagaccaaag cattgattga agcgcagggc tacgacatta tctacggaga
    19801 taccgactcg acttttgtct ggctaaaagg tgcacattcg gaagaagagg cggcgaaaat
    19861 cggtcgtgca ctggtgcagc acgttaacgc ctggtgggcg gaaacgctgc aaaaacaacg
    19921 gctgaccagc gcattagaac tggagtatga aactcatttc tgccgttttc ttatgccaac
    19981 cattcgcgga gctgataccg gcagtaaaaa gcgctacgcc gggctgattc aggagggcga
    20041 caagcagcgg atggtgttta aagggctgga aaccgtgcgc accgactgga cgccgctggc
    20101 ccagcagttt cagcaggagc tgtacctgcg tatcttccgc aacgagccat atcaggagta
    20161 cgtccgccaa accatcgaca aactgatggc gggtgaactg gatgcgcggc tggtttaccg
    20221 taaacgcctt cgccgtccgc tgagcgaata tcagcgtaat gttccgcctc atgtacgcgc
    20281 cgctcgcctt gccgatgaag aaaatcaaaa gcgcggtcgc cccttgcaat atcagaatcg
    20341 cggcaccatt aagtacgtat ggaccaccaa cggcccggag ccgctggact accaacgttc
    20401 accactggat tacgaacact atctgacccg ccagctacaa cccgtggcgg agggaatact
    20461 cccttttatt gaggataatt ttgctacact tatgaccggg caacttgggc tattttgagc
    20521 aaaaaaaaga gttcgccaga taccattttg atgcgtgacg aatgctttgc catccagtac
    20581 catagcgccc tttccattcc tggacctgaa taacaccact acctcataag cacggtagcg
    20641 ggtggttatt gcctgcaatt aaagatatag agccgaacac atatgccttt tacacttggt
    20701 caacgctgga tcagcgatac agaaagcgaa ttgggacttg gaaccgttgt cgcggtggat
    20761 gcgcgaactg tcactttact tttcccatct actggtgaaa accgtctgta cgcacgcagt
    20821 gattcccccg tgacccgcgt gatgttcaac cctggtgata ccattaccag ccatgacggc
    20881 tggcagatgc aagtcgaaga agtaaaagaa gaaaatggct tgctgaccta tatcggtact
    20941 cgcctggata ctgaagagtc cggcgtagcc ctgcgtgaag ttttccttga tagcaaactg
    21001 gtgttcagca aaccgcagga ccgtctgttt gccgggcaga ttgaccgtat ggaccgcttt
    21061 gcgctgcgtt atcgcgcgcg taagtattcc agcgaacagt tccgtatgcc gtacagcggc
    21121 ctgcgcggtc agcgtaccag cctgatcccg catcagctca acatcgctca tgatgttggt
    21181 cgccgccacg cgccgcgcgt cctgctggct gacgaagtgg gtttagggaa aaccattgaa
    21241 gccgggatga tcctgcatca gcaactgctc tctggcgctg ctgaacgtgt gctgattatc
    21301 gtcccggaaa ccttacagca tcagtggctg gtagaaatgc tgcgccgttt caacctgcgc
    21361 tttgctctgt ttgatgatga gcgttatgcc gaagctcagc acgatgctta caacccgttt
    21421 gacaccgaac agctggtgat ttgctcgctg gattttgccc gtcgtagcaa acagcgcttg
    21481 gaacatctct gtgaagccga atgggacctg ctggtggtcg atgaagcgca tcacctggtg
    21541 tggagcgaag atgcgccaag ccgtgaatat caggccattg aacaactggc agagcacgtg
    21601 ccgggcgttc tgctgctgac cgcgaccccg gaacagctgg ggatggaaag ccacttcgcc
    21661 cgtctgcgtc tgctggaccc gaaccgtttc cacgattttg cgcagttcgt tgaagagcag
    21721 aaaaattatc gtccggttgc ggacgccgtt gccatgctgc tggcaggtaa caaactgagc
    21781 aatgacgaac tgaacatgct cggcgagatg atcggcgagc aggatatcga gccgctgttg
    21841 caggcagcaa acagcgacag cgaagatgcc cagagcgccc gtcaggagct ggtttcgatg
    21901 ctgatggatc gccacggcac cagccgcgtg ctgttccgta acacgcgtaa cggtgtgaaa
    21961 ggattcccga aacgcgagct gcacaccatt aagctgccgc taccgacgca gtatcagacg
    22021 gctattaaag tctccggcat tatgggcgca cgtaaaagtg cggaagatcg tgctcgcgat
    22081 atgctctacc cggagcgtat ttatcaggaa tttgaaggtg ataacgccac ctggtggaac
    22141 ttcgatccgc gcgttgagtg gctgatgggc tacctgacca gccatcgctc tcagaaagtg
    22201 ctggtgatct gcgccaaagc tgccactgcg ctgcaactgg agcaggtact gcgcgaacgt
    22261 gaaggtattc gcgctgcggt gttccacgaa ggtatgtcga ttatcgaacg tgaccgcgct
    22321 gccgcctggt ttgccgaaga agacaccggc gcacaggtac tgctgtgctc agaaatcggt
    22381 tctgaaggac gtaactttca gttcgccagt cacatggtga tgtttgacct gccattcaac
    22441 ccggatctac tggagcagcg tattggtcgt ctggatcgta tcggccaggc gcacgatatt
    22501 cagatccatg tgccttatct ggagaaaacc gctcagtcgg tgctggtgcg ctggtatcac
    22561 gaaggtctgg atgcatttga gcacacctgc ccgaccggac gcactattta cgatagcgta
    22621 tacaacgatc tgattaacta tctggcttca ccggatcaaa ccgaaggctt tgacgatctg
    22681 atcaaaagct gccgcgagca acatgaagcg ctgaaagcac agctggaaca gggtcgtgac
    22741 cgcctgctgg aaatccactc caacggtggc gaaaaagccc aggcactggc agaaagcatt
    22801 gaagagcagg atgacgatac caacctgatc gccttcgcca tgaacctgtt cgatattatc
    22861 ggtatcaatc aggacgatcg cggcgacaac atgatcgtgc tgacgccgtc cgatcatatg
    22921 ctggtgccgg acttccctgg cctgtcggaa gatggcatca ccatcacctt tgatcgtgaa
    22981 gtggcgctgg cgcgtgaaga tgcacagttt attacctggg agcatccgct gatccgcaac
    23041 ggtctggatc tgatcctttc tggcgatacc ggtagcagca cgatttcact gttaaaaaac
    23101 aaagcgttgc cggtaggtac gctgttggtg gaactgattt atgtggttga agcccaggct
    23161 ccgaagcagt tgcagctcaa ccgcttcctg ccaccgacgc cggtacgtat gctgctggat
    23221 aaaaacggca acaacctggc ggcgcaggta gagtttgaaa cctttaaccg ccagcttaac
    23281 gcggttaacc gtcacaccgg cagcaaactg gttaacgccg tgcagcagga tgttcacgct
    23341 atccttcaac tgggtgaagc gcagatcgag aaatctgccc gtgcattgat tgatgcagcg
    23401 cgtaacgaag ccgacgaaaa actgtctgcc gagctgtctc gtctggaagc tctgcgtgca
    23461 gtgaacccga acattcgtga cgacgaactg accgccattg agagcaaccg tcagcaggta
    23521 atggaaagcc tggatcaggc aggttggcgt ctggatgccc tgcgtttgat cgttgtaacg
    23581 catcagtaac ggagccgaaa atggggatgg aaaactacaa tccaccgcag gaaccctggt
    23641 tggttatcct gtatcaggat gaccatatta tggtggtcaa caagccgagc ggtttgttgt
    23701 cagtgccggg tcgtctggaa gagcacaaag acagcgtgat gacgcgcatt cagcgtgatt
    23761 atccgcaggc agaatcggtg catcgtctgg atatggctac cagcggcgtg attgtagtgg
    23821 cgctgaccaa agccgcggag cgggagttaa aacgccagtt ccgcgagcgc gagccgaaaa
    23881 agcagtatgt ggcccgcgtc tgggggcatc catcccccgc agaaggtctg gtggatctgc
    23941 cgctgatttg cgactggcca aaccgcccga aacagaaagt ctgttacgaa acgggtaaac
    24001 ctgcgcagac ggaatatgaa gtggtggagt atgcggcgga taacacggca agagtggtgt
    24061 taaaaccgat taccgggcgt tcgcatcaat tgcgtgtgca tatgctggcg ctggggcatc
    24121 cgattctcgg cgatcgtttt tatgcatcac cagaagcgag agcgatggca ccgcgtttgt
    24181 tgctacatgc agagatgttg acgattaccc atccggcgta tggcaatagt atgacgttta
    24241 aagcgccagc ggatttttaa tcgtcacgct ggcgcagttc ggtaggcgga taaggcgttc
    24301 acgccgcatc cggcaattgt gcgccgattg cctgatgcga cgcgggccgc gtcttatcag
    24361 gcctacagtt taaaacttat ttaaaccctt tctgctgctt tatcagctca tatgcctgct
    24421 gaatttcctg cgctttctgc ttcgccatct ccatcatctc aggcggcaaa cctttcgcca
    24481 ccagcttatc gggatggtgt tcactcatca gcttacggta ggcacgtttg atggtggtcg
    24541 catcatccgt cggcttcacg cccagcacat tacaggcatc ttccagcgtc gggccacgct
    24601 gcgcttgctg ccagttacca ccgccagatt gctgctgata accgccgcca aactgtgcac
    24661 cgccctgcat catgcgcaaa aactggtcaa actgggcgcg ggagatccct aattcttcag
    24721 caatgacata cagcaccgcc cgttcattcg ggtgcagtga accatcagca aacgccgcct
    24781 gaatctggat ctccagaaac atacgaatta agtcaaaacg accaaagcag acactgcgaa
    24841 actggcgcat cttttcgcgc agcgggtaat tatctgattt tcccacccgg aacgcgtttt
    24901 gcgccgcagt acgggaagcg ccatgaaggt tcattcggtc catcaactgg ctggcgatat
    24961 gaatatcagc ctccgtgacg cgacctttgg atttggttaa atgccccatc acttcaaaag
    25021 tggtggcaaa aaacagcgcc tgacgctcac gctggttggc gaaccacgcc attttacggc
    25081 tacgggcttt atcaaacata tggccaatta acaggcctaa cactacgccc caaaagccgc
    25141 cgcccatcag taaggccacg gccacgccaa tgatttttcc ccaatactgc atatattccc
    25201 caaatcgaca cacggatatc agggctatct cccacaatat aaaggtgctt ttaccgtttt
    25261 ccggctgcgg tcaattgtgg gacatcgcct ataatttgca ttatcatacc tgtcattcaa
    25321 tgccgtgcct aacaccactg acgctattcg gacaggatta acactagcgt agagatgacg
    25381 agtacgttag tctctgaccg tttgtcacgc gcaacgttac cgatgatgga acaataaaat
    25441 caacgtatga aaaaacgtat ccccactctc ctggccacca tgattgccac cgccctttat
    25501 agtcaacagg gactggcagc cgacctcgcc tcacagtgca tgttgggcgt gccaagctat
    25561 gaccgtcctc tggtacaggg cgataccaat gacttacccg tgactatcaa tgctgaccac
    25621 gcgaaagggg actacccgga tgacgccgtg tttactggca gcgtggatat catgcagggt
    25681 aacagccgtc tgcaggccga cgaagtgcag ctccatcaaa aagaggcacc aggacaaccg
    25741 gagccggtac gtaccgttga tgcgctcggt aatgtccatt acgacgataa ccaggtgatc
    25801 ctcaaagggc cgaaaggctg ggcgaatctg aacaccaaag ataccaacgt ctgggaaggt
    25861 gattaccaga tggtgggtcg ccagggtcgc ggtaaagcgg acctgatgaa acaacgtggc
    25921 gaaaaccgct ataccattct ggataacggt agctttacct cctgtctgcc gggttctgac
    25981 acctggagcg tggtaggtag cgaaattatt catgaccgcg aagaacaagt tgctgagatc
    26041 tggaacgccc gctttaaggt gggtccggta ccgatctttt atagccccta tttgcagttg
    26101 ccggtgggtg acaaacgtcg ctctggtttc ttgatcccga acgccaagta caccaccacc
    26161 aactactttg agttctacct gccatattac tggaacatcg cgccaaatat ggatgccacc
    26221 atcacgccgc attatatgca tcgtcgtggc aacatcatgt gggagaacga attccgctac
    26281 ctctcccagg cgggcgctgg cttgatggaa ctggactatc tgccttcaga taaagtctat
    26341 gaagatgaac acccgaacga tgacagttca cgtcgttggt tattctactg gaaccactcc
    26401 ggggtcatgg atcaggtgtg gcgtttcaac gtcgactaca ccaaggtcag cgatcctagc
    26461 tacttcaatg atttcgataa caagtacggt tccagtactg acggctacgc aacgcaaaaa
    26521 ttcagcgttg gctatgcggt gcaaaacttc aatgccaccg tttcaaccaa gcagttccag
    26581 gttttcagcg aacagaacac cagtagctac tcggcagagc cgcagttaga cgttaattac
    26641 taccagaatg atgttggtcc gtttgatacg cgtatttacg gccaggcagt gcactttgtt
    26701 aacaccagag acgacatgcc tgaagcaacc cgtgttcacc tggaaccgac catcaatttg
    26761 ccgctctcta ataactgggg cagcatcaat accgaagcga agtttctggc aacccattat
    26821 cagcaaacca atcttgactg gtataactcc agaaacacga ccaagctgga cgaatccgtt
    26881 aaccgcgtaa tgccgcaatt caaagttgac ggcaaaatgg tctttgaacg cgatatggaa
    26941 atgctggctc cgggttatac ccaaacgctg gaaccgcgcg cgcagtattt gtacgtgccg
    27001 tatcgcgatc agagcgacat ctataactac gactcgtctc tgctgcaatc tgactactct
    27061 ggcctgttcc gggaccggac ttacggcggt cttgaccgta ttgcctccgc taaccaagtg
    27121 acgaccggtg tcacatctcg catatatgat gatgctgccg ttgaacgttt taatatttcc
    27181 gttggtcaaa tctactattt cacggagtct cgcactggcg atgacaacat aacatgggag
    27241 aatgacgaca aaacgggctc actggtgtgg gcaggcgaca cctactggcg tatctccgag
    27301 cgttggggat tgcgtggcgg gattcagtac gatacacgtc tggataacgt agcgaccagt
    27361 aactccagca ttgaataccg tcgggatgaa gaccgtctgg tacagctgaa ttaccattac
    27421 gccagcccgg aatatattca ggctacgctg cctaagtact attccactgc tgagcaatat
    27481 aagaatggta tttcgcaggt aggtgctgtc gccagcaggc caattgccga tcgttggtcc
    27541 attgttgggg cctactacta cgacaccaat gcgaacaagc aagccgactc tatgttaggt
    27601 gtgcaataca gctcctgctg ctatgcaatt cgcgtcggtt acgagcggaa gctgaacggt
    27661 tgggataacg ataaacaaca tgcggtatat gacaacgcaa tcggctttaa catcgaactt
    27721 cgcggcctga gctccaacta cggtctgggt acgcaagaga tgctgcgttc gaacattctg
    27781 ccgtatcaaa acactttgtg atttgttgat ttaccacgta atccgcagtg cggttaattg
    27841 aaatggaaaa agtatgaaga actggaaaac gctgcttctc ggtatcgcca tgatcgcgaa
    27901 taccagtttc gctgcccccc aggtagtcga taaagtcgca gccgtcgtca ataacggcgt
    27961 cgtgctggaa agcgacgttg atggattaat gcagtcggta aaactgaacg ctgcgcaggc
    28021 aaggcagcag cttcctgatg acgcgacgct gcgccaccaa atcatggaac gtttgatcat
    28081 ggatcaaatc atcctgcaga tggggcagaa aatgggagtg aaaatctccg atgagcagct
    28141 ggatcaggcg attgctaaca tcgcgaaaca gaacaacatg acgctggatc agatgcgcag
    28201 ccgtctggct tacgatggtc tgaactacaa cacttatcgt aaccagatcc gcaaagagat
    28261 gattatctct gaagtgcgta acaacgaggt gcgtcgtcgc atcaccatcc tgccacagga
    28321 agtcgaatcc ctggcgcagc aggtgggtaa ccaaaacgac gccagcactg agctgaacct
    28381 gagccacatc ctgatcccgc tgccggaaaa cccgacctct gatcaggtga acgaagcgga
    28441 aagccaggcg cgcgccattg tcgatcaggc gcgtaacggc gctgatttcg gtaagctggc
    28501 gattgctcat tctgccgacc agcaggcgct gaacggcggc cagatggggt ggggccgtat
    28561 tcaggagttg ccggggatct tcgcccaggc attaagcacc gcgaagaaag gcgacattgt
    28621 tggcccgatt cgttccggcg ttggcttcca tattctgaaa gttaacgacc tgcgcggcga
    28681 aagcaaaaat atctcggtga ccgaagttca tgctcgccat attctgctga aaccgtcgcc
    28741 gatcatgact gacgaacagg cccgcgtgaa actggagcaa attgctgctg atatcaagag
    28801 tggtaaaacg acttttgctg ccgcagcgaa agagttctcg caggatccag gttctgctaa
    28861 ccagggcggt gatctcggct gggctacacc agatattttc gatccagcct tccgtgacgc
    28921 cctgacccgc ctgaacaaag gtcaaatgag tgcaccggtt cactcttcat tcggctggca
    28981 tttaatcgaa ctgctggata cccgtaatgt cgataaaacc gacgctgcgc agaaagatcg
    29041 tgcataccgc atgctgatga accgtaagtt ctcggaagaa gcagcaagct ggatgcagga
    29101 acaacgtgcc agcgcctacg ttaaaatcct gagcaactaa tggttaaaac ccaacgtgtt
    29161 gtgatcactc ccggcgagcc cgccgggatt ggcccggact tagttgtcca gcttgcacag
    29221 cgtgagtggc cggtcgaact ggttgtttgt gccgatgcca ctctccttac cgaccgggca
    29281 gcgatgctcg gtttgccgct caccctccgc ccttattccc ccaactcccc tgcacaaccg
    29341 caaactacgg gcacattaac gctacttcct gtcgcgctac gtgaatctgt cactgcgggg
    29401 cagttagcga ttgaaaatgg gcattacgtg gtggagacgc tggcgcgagc gtgtgatggc
    29461 tgtctgaacg gtgaatttgc tgcgctgatc acaggccccg tgcataaagg cgtcattaac
    29521 gacgcaagca ttccgtttac cggtcatacc gagtttttcg aagagcgttc gcaggcgaaa
    29581 aaagtggtga tgatgctggc gaccgaagaa cttcgcgtgg cgctggcaac gacgcattta
    29641 ccgctgcgcg atatcgcaga tgctatcacc cctgcacttt tgcacgaagt gattgctatt
    29701 ttgcatcacg atttgcgtac aaaatttggt attgccgaac cgcgcattct ggtctgcggg
    29761 ctgaatccgc acgcgggcga aggcggtcat atgggtacgg aagagataga caccattatt
    29821 ccggtgctcg acgagctgcg ggcgcagggg atgaaactca acgggccgct gcctgccgat
    29881 accctgtttc agccgaaata tctcgataac gccgacgccg tgctggcgat gtaccacgat
    29941 cagggtcttc ccgtgctaaa ataccagggc ttcgggcgcg gtgtgaacat tacgctgggc
    30001 ctgcccttta ttcgcacatc agtggaccac ggcaccgcgc ttgaactggc gggacgtggc
    30061 gaagccgatg tcggcagttt tattacggcg cttaatctcg ccatcaaaat gattgttaac
    30121 acccaatgaa taatcgagtc caccagggcc acttagcccg taaacgcttc gggcaaaact
    30181 ttctcaacga tcagttcgtg atcgacagca ttgtttctgc cattaacccg caaaagggcc
    30241 aggcaatggt cgaaatcggc cccggtctgg cggcattgac cgagccggtc ggcgaacgtc
    30301 tggaccagct gacggtcatc gaacttgacc gcgatctggc ggcacgtctg caaacgcatc
    30361 cattcttagg cccgaaactg acgatttatc agcaggatgc gatgaccttt aactttggcg
    30421 aactggccga gaaaatgggt cagccgctgc gtgttttcgg caacctgcct tataacatct
    30481 ccacgccgtt gatgttccat ctgtttagct atactgatgc cattgccgac atgcacttta
    30541 tgttgcaaaa agaggtggtg aatcgtctgg ttgcaggacc gaacagcaaa gcgtatggtc
    30601 gattaagcgt catggcgcaa tactattgca acgtgatccc ggtactggaa gttccgccgt
    30661 cagcctttac accaccaccc aaagtggatt ccgccgtcgt gcgcctggtc cctcatgcaa
    30721 cgatgcctca cccggttaaa gatattcgcg tgttgagccg catcaccacc gaagccttta
    30781 accagcgtcg taaaaccatt cgtaacagcc tcggcaacct gtttagcgtc gaggtgttaa
    30841 ccggaatggg gatcgacccg gcgatgcgag cggaaaatat ctctgtcgcg caatattgcc
    30901 agatggcgaa ctatctggcg gagaacgcgc ctttgcagga gagttaacga tgatcaattc
    30961 gccccgagtg tgtattcagg ttcaaagcgt ctacattgag gcacaatctt cacctgataa
    31021 tgaacgttac gtttttgctt ataccgtaac catacgcaat ctggggcgag cgccagtgca
    31081 gttgttgggg cgttactggc tgatcaccaa tggcaatggc cgtgaaaccg aagtccaggg
    31141 cgaaggagtg gttggcgtcc agccacttat cgcgcctggc gaagagtacc agtacaccag
    31201 cggtgcaatc attgaaaccc cgctgggcac catgcagggt cactacgaaa tgatcgatga
    31261 aaatggcgtc cctttcagca tcgacattcc cgtattccga ctcgccgttc ccacactcat
    31321 tcattaaaag aatatggcga cataccttat tggcgacgtt catggttgtt acgatgaact
    31381 gatcgcattg ctgcataaag tagaatttac ccccgggaaa gataccctct ggctgacggg
    31441 cgatctggtc gcgcgcggcc cgggttcgct ggatgttctg cgctatgtga aatccttagg
    31501 cgacagcgta cgtctggtgc tgggcaatca cgatctgcat ctgctggcgg tatttgccgg
    31561 gatcagccgc aataaaccga aagatcgcct gacaccgctg ctggaagcgc cggatgccga
    31621 cgagctgctt aactggctgc gtcgccagcc tctgctgcaa atcgacgaag agaaaaagct
    31681 ggtgatggcc cacgcaggga tcacgccgca gtgggatctg cagaccgcca aagagtgcgc
    31741 gcgcgatgta gaagcggtgc tgtcgagtga ctcctatccc ttctttcttg acgctatgta
    31801 cggcgatatg ccaaataact ggtcaccgga attgcggggg ctgggaagac tgcgttttat
    31861 caccaacgcc tttacccgta tgcgtttttg cttcccgaac ggtcaactgg atatgtacag
    31921 caaagaatcg ccggaagaag cccctgcccc actgaaaccg tggtttgcga ttcctggccc
    31981 tgtcgctgaa gaatacagca tcgcctttgg tcactgggca tcgctggaag gcaaaggtac
    32041 gccggaaggt atttacgcgc tggataccgg ctgctgctgg ggtgggtcat taacctgcct
    32101 gcgctgggaa gataaacagt attttgtcca gccgtcgaac cggcataagg atttgggcga
    32161 agcggcggcg tcttaaacac agcctgatac aggaaggccg gataagacgc gaccggcgtc
    32221 gcatccggca ctagccgtaa attctataca aacttaccgc cgctccagaa tctcaaagca
    32281 atagctgtga gagttctgcg catcggcgtc gtggaattcg ctgaataccg attcccagtc
    32341 atccggctcg taatccggga aatgggtgtc gccttccact tctgcgtcga tatgcgtcag
    32401 atacagtttt tgcgcttttg gcaggaactg ctcataaacg cgaccgccgc caatcaccat
    32461 gatttctggt acgtcaccac acgccgcaat ggcttcatcc accgatttca cccacgttac
    32521 gcgatcgtcc gtacccggtt gactgctgag gataatattt ttgcgtcctg gcaacggacg
    32581 accgattgat tcccaagtat ggcggcccat aatcacgggt ttatttaagg tgttgcgttt
    32641 aaaccaggcg agatcggcag gcaggttcca cggcatggcg ttttccatgc cgataacgcg
    32701 atctaccgct aacgccgcaa tcagactgat cattgagatt tcccgataaa aaaattgtcg
    32761 ccactatacg taaagcgtaa accgtcgtcg actggtgcga ggatgatgtt gaggaaaatt
    32821 ttatattctg ctggcgagtc cacgctctcc ccctggactc gccgcattac aatgaaacaa
    32881 aaacaaacag ttagctgtaa agtgtgattt acgtcactct ttattaagat gagggtttcg
    32941 tttccggttc atccgccatg ttgccggtat gtttaccttc ttctgttccc tgccagccat
    33001 gtcgttgaat taatgacaga tgttcgcggt cctcggtgat gatctcactt aacatcgcgc
    33061 tggtgcgttt atagaccgcc gcgcgggctt tggtgtcatt ctcaaccatt gccatctctt
    33121 ccaccatctg aatattaaag cggcggaaca catcggcacg ttctcgcgct tcatacggcc
    33181 ccagacctaa actttccagc gccagacgcc cggttttcag cgcaccttca aaggtttcac
    33241 gctccggctt ttcaacgcct gcctgacgca aacgaatgta gtgatcgaca tcgcgggcgc
    33301 gggcaataat ctgcaaatgc gggaaatgtt ctttcaccat ctccgtcagt tgcaggttgg
    33361 tttgcggatc gtcaatagcg ttaatcagca cttccgcttt cgccgctccg gcagattcca
    33421 gtaaatccat ccgcgtggca tcgccataaa acactttcat accaaattta cgcagcgttt
    33481 cgatatggtc cggatcgtga tcgagcacca ccattttcac cccgctggag agcagtaaac
    33541 gtccggtaat ctgtccaaaa cgaccgaatc cggcgataat cacgcgcggc tgttcttcat
    33601 cgatctcatc ggcttcacgc gcctcctccg tagaagattg ctcaaggcga ttgaggatca
    33661 ccagcagaat cggcgttgct gccatcgaca gcgccaccgc cagggtcagc gatttcgccc
    33721 actccggctc cagcacattc gccatctgcg ccgcaccaaa taccacaaag gcaaactcac
    33781 tgccctgccc taacaacacc gcaaaccagc gacgctgttt atttggcacc tgcaacggtc
    33841 gggcaatcag ccacagcatg gcgattttga tgatgaggaa accgagcagc aaaatgacaa
    33901 tgcgcaatgg gttttcaatc agcgtgccaa agtctatcga catgccaaca ccgatgaaaa
    33961 acagccccaa cagcaaacct ttaaacggtt cgatatcgct ttccaacgca tgacggtatt
    34021 ccgagcttgc cagcagtacg cccgccagaa acgcgcccat cgccatcgac aagccgacct
    34081 cttccagcag caaaccaaag ccaaacacaa ggaataacgc cacggcacta aacacttccc
    34141 gcaagccaga gcgggcaaca aaacgcagcg ccggacgcgt aacatagcgc cccagcaata
    34201 ccaccagtac cagcgcaccc gccactttta acgccgagag agcaaatgcg cccatcgtcg
    34261 tcgaggcact gctcgccgcc agtagcggga tcatcgccac cagcgggatt gccgcgatat
    34321 cctggaacag cagcactgca aaggcactgc ggcccatttg cgtcaccatc agattgcgtt
    34381 cattcatcgc ctgcatggca atcgccgttg atgagagcgc cagcgtcata ccgatcaatt
    34441 ccgcgacctg ccagcgcagc ccaagtaaca tacagaacag ccccagcagg ccaccgcaaa
    34501 tcaccatctg caatgcgcca ccgccgaaca ctgccgcacg cagcttccac agcctttgtg
    34561 gatcgagttc gaggccgata ataaacagca tcagcaccac cccaatctcg gcaaagtgca
    34621 gaatagattc ggcatcggtc accagtcgca gcccccacgg gccaataatg cagccggcga
    34681 tcaggtagcc aagtaccgat cccagaccaa gacgtaccgc aatgggtaca atcagcgccg
    34741 ccgatccgag gtagatcagc gcctgaacca gcgtatgact atccatgatg ggcctcctgc
    34801 cattccagca gacgttgctt atagtggcgc gcctgccctt cgagggtttc gtcgtcacaa
    34861 ataaaggtgc agtgcatggc aaacggtggc agccagttca gcccgcagta gattgccgtc
    34921 gcctgtagcg gctgcgacag cacatcaaag cccggatgcg caccaatttc aaaatggctt
    34981 tccccgccgc cggtcgtcac cgcccacagc aaatgtttgc catgcagcgc cgtgccgccg
    35041 tgaccgtaag cccagccgtg cgacaaaact ttatcgatcc aaagtttaag aagcggagga
    35101 atgctgtacc actgcatcgg gtgctgccag acgatcagat cggcgcgaga gagcgcctcc
    35161 tgctcggcgg caatatcgat attgaagtca ggatagagtt gataaagaga gcgaatttcg
    35221 acgccttcca gcgtccttgc ctgttcaagc atccgtttat tcgcatggga atgatgcgga
    35281 tacggatgcg cataaatgat gaggatcata gttattagcc cgcgtgttta cgcctttgtt
    35341 ttcccacaaa gtgtagtcat atattcacgc ggcttatagt caataatatt gattcagaaa
    35401 agagagaaat ctgaacaaat aatcccattc tctgcccgcg gatcgtcata ttgctggtct
    35461 gcgccagtga cattcctcga gtctccgggg caaacgctac ggaaatcaac aagccaaaca
    35521 gcgagatacc cgcccccatt agcatcgtgt tactgatacc gtaattattg ataaatatcg
    35581 gcagtgccca ggtcgaaaca atggtgccaa tacgacttaa ggacataatc acgcccacgg
    35641 cagaggcgcg gatatccgtc gggaagagtt cattaggata gagccactgc aaattacccg
    35701 gcccgccaga gaaaaaggca tacaccgcaa aggccatcac taccagccag atccccatat
    35761 ccgggattag ccccaaaacc gccaacgcca gcgtcatcat ggcaaagctg ccaatcaaca
    35821 atggacgccg tccggcagtg tttagccaca gcatcggcgg aatacagccg agcataaaga
    35881 acaggctaat caccacattc cctagtgccg cgtttttgcc aacccccaat cccaacgaac
    35941 caacgatttg cgggccaaag gtgtaaatgg cgaacattgg gatcacctgg caggtccaga
    36001 tggcggcaac aaacagaaca aaaggaaaat ggcggcgatt aaacagatcg cgaaaacggg
    36061 tttgctgcgg ctgctcttca tcgaaagcca ccggttcgcc aaacagtttt atcatcatct
    36121 cttcgcactc ttttactcgc cctttgcgta ataaccagcg gggagattct ggcagttcga
    36181 atcgaccaat caaaatcaac aaacaaggga tcgccgcgct acccagcatc cagcgccaac
    36241 caccttccac atcataaagc cagtagccga ccagatcggc gcaggtcgcg ccgacatacc
    36301 acatcgcggc aataaagctg atggaaaacg cccgctgacg ggtactggag aactcggtga
    36361 tcatcgaagt ggcgatggga taatctgcac cgatgacaat gccgatgagt acccgcatca
    36421 ccaacagttc gacgggggat gaaacacaca tcgtcgccac cgatatcacg ccgatggcga
    36481 tgatatcaat gaggaacatt ttgcgccgtc cgactttatc ggagatatag ccaaacagcg
    36541 atgtgccaac gaacagcccg gcgagcgttc ccgcgcccag caagccaatc cagtcagcgt
    36601 ccagtttcag cgccggagtc agttgctcca gcgccacgcc aatcattacc agtacataac
    36661 catccagaaa cggaccaccg cttccccaca gcaaaatgcg gcggtgaata gaggagaatt
    36721 tgagatcgtc aaagtttctg gacggttgca tgggttattc ctgttttttt gcaatgagag
    36781 tcagtacggc gttacgtatc aaaccaacat cagccgtaac ggaactccac accaaaggtg
    36841 ccgcgcgggt attcccactg ttccagcgcc gaccccagcc ccagaatgcg acaggtgccg
    36901 cactccagac atccggcgta atcgaagcgc acactgccgt catcctgctt cttgtacaga
    36961 cctgcggggc acgctttcac cagcagctcc agcacctgtt tatcagcatc ggccttcaca
    37021 acaatgtgcg gatgctcttc atcgacattg aatttattga cgcccagttt gacgtccaca
    37081 ttgacgggag aagtcataaa acggtcactc ctttcatgcc atccttgatc agattgatga
    37141 agcccacttt cttgccgtgg cggaggattt tcttgcgcat cagttccggc gcactgccgt
    37201 caatggtgaa caggtcacgc gccacgccca ccgccagttc cgggttgccg ctaaacatgc
    37261 gtgggttatc aaggaacgcc ggtagtttct ggtacatgcg catatcgcgc agcgggccac
    37321 tctccagatg ctgacgatat tccgcgagtt tttgcttact gaaatcgtcg cttttcatcg
    37381 ctgaaagcac ggtttttgct gcagcttccc cggcggcaat cgccagatcc ataccgcgaa
    37441 tggtaaaacc gaggttcata cacattccgg cggcatcacc ggcaatcaat acgccgtcac
    37501 cgaccagctc cggcagcatg ttgatgcctg cttccggcac tacgtgagcg gaatattcca
    37561 ccagcttgcc gcccgcgatc agcggtgcaa cggccggatg ctgtttgaaa tcttccagca
    37621 tttgcggcac cgattttttc gcgtcatgca gatgatgcag accacaaacc agccccagcg
    37681 acagggtatt ttcattggta taaaggaagc cgccgcccat caggccatcg gtgggtgatc
    37741 ccgcaaacag acaagccgcg ccctgattac cctgcaactg aaaacggtct tcgattaccg
    37801 acttcggtaa ctcgatcagt tccttcacgc caaccgccac atccgtcggt ttaacgcgtt
    37861 ttgccatccc caacttttcg gcaaggatgg agttcacccc atcagcaagg agcaccgttt
    37921 tcgcttcaat cacatcacca tcggcttcta caccgacgac tttgccatcg cgctgtacga
    37981 ggttatcgac gcggatcccg gtaattaact gcgcgcccgc ttcttcggcc tgctccatca
    38041 accaggcatc aaatttactg cgcaaaacgg agtaagaacg ctgggatggc gaggtttcgt
    38101 caccgttgca gtagtccata gtcatcgctg acttttccgt cataaacgcg agtttttcat
    38161 gggtgatcag gcgttctacg ggggcggaat cggcgaaacc tggaataatg tgttccagac
    38221 tgtgggcata gagacgcccg ccagtaacgt tcttggcacc tgcggaattg ccacgctcga
    38281 taactaacac ttgcgcccct tcgcgggcga gcaccagtgc ggcaaccgaa ccggcaagcc
    38341 ctgcaccgac gatgatggcg tcaaagatat cttcggacat aagagctccc tgtcagcggc
    38401 agagccgtaa tgcagccctg ccagagtgga tcaacgcgct aaagctgcgg tcagtgccgg
    38461 aaggatcttc acggcatcgc caacaatgcc gtaatccgcg tactggaaga tcggcgcatt
    38521 tttatcttta ttgatggcga aaatggtttg cgacgcgtta gcgccaacca tgtgctggat
    38581 ctacccggag atccccaccg ccaggtacag ttcaggtttc agcatcaggt tggagatacc
    38641 gacatagcgt tcgtgctcca tccatttttc gttttctgcc accggacgag aacaggccaa
    38701 ctccgcacct atcgccttgc aaagctgttc tgccagcgca atgttctctt tgctgccaat
    38761 gccgcgaccg acgctgacca ccagacgggc tttgtcgaga tcgacgctgt tgctctggcg
    38821 cgcctgggtt gccgtgcggg taatcgccac agccggagcc tgccactcca cggtgtgcgt
    38881 ttcgccagtg cgtgacgcgt ctggctgagc cgcatcgaac gtgccgctgc tgatggtcag
    38941 taccgcatac ggcgtggcaa tgcgttcttc gccaatcgcc agaccgccgt aaaccatgtg
    39001 tttcactgtt gctttaccgt cctgtacgct gacggtgctg gcatcgttag acaccgccgc
    39061 tttaaggcga taacccagtt ttgccgccag taatttgccg cgacgggtgt ttggcaacag
    39121 caccagaccg tcagcgccgt gctggcgaat agtgtcagcc atgacaccgg cgtaatcttc
    39181 gatcatccga tcgtccggtt tgccgtttaa tttccagaca tgattagcgc cgagctggat
    39241 tgcctgtgcg ccgtcggcat cattgaggac aaaggcattg atttgattag ctaaagcctg
    39301 cgcaccgttc atcagttccg gcagacgaga gggggtatcg ctgaataccc agacttgaga
    39361 aaacgtgttc atagcatccc ctgtaattaa atgactttgc gaagattttc agcaaatgcg
    39421 gcgatctgtt cttcgccgtc gccttcaatc acgatgcgct gacgctcgcg ctgtttcggc
    39481 gcggcaacct gttgttcaga ccaggcgtcc acggcgttaa aaccaatatc cgccgccgac
    39541 catacctgga ctggcttttt cgccgcgccg agaatggctt tcatcgaagg aatttgtggg
    39601 gagttgatat cagtggaaac agcaacaacc gcaggcagcg gaatgcttaa ggtttcggtt
    39661 tcatcttcca gttcgcgctc aacggtgagg gtatctgccg tcagggagat aattttgctg
    39721 acgccgttaa ctgccggaat attgaggatt tcgcccacca gcagaccaac ctgctgggca
    39781 taaaggtcgg aagaaccatc gccacagagg atcagatcaa agcctgcttt ctgggcggct
    39841 gcagccagtg cgctcgccgt ttgttgcggc agtgcctgct cgaactggtc atcaatcacc
    39901 acaatcagtt catccgggcc gcgcgatagc acatctttac gccctttggc gttggtcagg
    39961 gctttaccgc ccacacttaa ggctgtcacc tgcgcctctg ctgcctgttg ctttagctgg
    40021 caagccgctt caatagcgtt gagatcgtat tggcttattt tggcatcggc tttgctgaag
    40081 tctaatgaac catcagcatt attgaccgca atatcctgtt catcaggcac gcacttatag
    40141 caagtaataa tcttcattgc atctccagaa atcatgaagg aaacgtcgtt catatcggtg
    40201 gtgttaatta cagatatcac gccctttaat ttggaaataa aactttaatc accaatattg
    40261 aaagtcagat gctatttttt tacccacaaa agaatataac aaactgatta ataaccaaaa
    40321 ttaacgaaaa acgccctctg ttcactcgaa acaaaaatgt gataccaatc acagaataca
    40381 gcttattgaa tacccattat gagttagcca ttaacgcgtc cacgatgtta ataataatta
    40441 tattaaatgt taacaaaaat aaaacaaacg ggaacgcaaa ataaatattc gttttcacag
    40501 tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa tagaaccgaa ggttttcttt
    40561 ccgccattaa taatcgtcgg catactttgt tggcttacag tcagagatct ggatgcggcg
    40621 aatgtcgtta ttaatgctgt attcagttac gtcaccaatg tatggggatg ggcatttgaa
    40681 tggtatatgg tggtgatgct tttcggttgg ttctggctgg tgtttggccc gtatgccaaa
    40741 aagcgtttag gtaacgaacc gccagaattt agcaccgcca gttggatctt tatgatgttc
    40801 gcctcctgta cgtctgctgc cgtactgttc tggggatcga ttgagatcta ctactacatc
    40861 tccaccccgc cgtttggctt agaaccgaac tcgacaggag cgaaagagtt ggggctggct
    40921 tacagcttgt tccactgggg acctctgccg tgggccactt acagcttcct ttcagtcgcc
    40981 ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc gccccagctc gacactggtg
    41041 ccgctggcag gtgaaaaaca cgccaaaggg ttgttcggta ccatcgtcga caacttctat
    41101 ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc ttgccacgcc gctggtgacc
    41161 gagtgtatgc aatggttgtt tggcattccg cataccctgc aactggacgc tatcatcatt
    41221 acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg gtctgcaaaa aggggtacgt
    41281 atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc tgggttgggt gttcatcgtc
    41341 agcggtgcca gcttcatcat gaactacttc accgattcgg tggggatgtt gctgatgtat
    41401 ctgccgcgca tgttgttcta taccgatccc atcgctaaag gcggcttccc gcagggctgg
    41461 accgtgttct actgggcatg gtgggtgatt tacgccatcc agatgagtat cttcctcgcc
    41521 cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg gcatggtgat ggggctgaca
    41581 gcatccacct ggatcctgtg gaccgtgctc ggtagtaaca ctctgctgtt gatggataaa
    41641 aacatcatca acattccaaa tctgatcgaa cagtacggtg tggcgcgcgc catcatcgaa
    41701 acctgggccg ctctgccact cagcaccgcc accatgtggg gcttcttcat cctctgcttt
    41761 attgccaccg ttacgctggt taacgcctgc tcttataccc tggcgatgtc tacttgccgc
    41821 gaagtacgcg atggtgaaga accaccgctg ctggtgcgta tcggttggtc aattctggtt
    41881 ggcattatcg gtattgttct gctggcgctc ggcggcctga aaccgattca aaccgccatt
    41941 atcgccggag gatgcccgct gttcttcgtc aacattatgg tgacgctctc ctttattaaa
    42001 gacgcgaaac agaactggaa agattaatta acccccaaaa tatcaagagg ttgaaagatg
    42061 gattttaatt taaatgatga gcaggaactg tttgtcgccg gtatccgcga actgatggcc
    42121 agcgaaaact gggaggccta ttttgccgag tgcgaccgtg acagcgtcta cccggaacgt
    42181 tttgtcaaag cactggcgga tatgggtatc gacagtctgc tgatccctga agagcacggt
    42241 ggtctggacg cggggtttgt tactctcgcc gccgtgtgga tggagctggg acgtctgggg
    42301 gcaccaacct atgtactgta ccagctgccg ggcgggttca ataccttcct gcgcgaaggc
    42361 acacaagagc agatcgacaa gattatggct ttccgcggca ccggtaagca gatgtggaac
    42421 tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta gcctgaaaac gacttatacc
    42481 cgtagaaatg gtaagattta tcttaatggt agtaagtgtt ttattaccag tagcgcctac
    42541 accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc cggacaaacc tgtctacacc
    42601 gaatggtttg ttgatatgag caaaccgggc atcaaagtga ccaaacttga gaagctcggt
    42661 ctgcgtatgg atagctgctg tgaaatcacc tttgacgatg tggaactgga cgagaaagac
    42721 atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag aagagttcga ccatgaacgt
    42781 ttcctggtag ccctgaccaa ctacggtacg gcgatgtgcg cctttgaaga tgctgcgcgc
    42841 tacgccaacc agcgcgtgca gtttggcgag gctattggtc gtttccagtt gattcaggaa
    42901 aaattcgccc acatggcgat caaattaaac tccatgaaaa acatgctgta tgaagcagcg
    42961 tggaaagcag acaacggcac catcacctct ggcgatgcag cgatgtgcaa atacttctgc
    43021 gccaatgcgg catttgaagt ggtggatagc gcaatgcagg tgctgggcgg tgtcgggatt
    43081 gcgggcaacc accgcatcag ccgcttctgg cgtgacctgc gtgtagaccg cgtttccgga
    43141 ggatctgacg aaatgcagat cctgacgctg ggtcgtgcgg tgctgaagca ataccgctaa
    43201 gtccgtgcgc tgcctgatgc gacgctgacg cgtcttatca ggcctacggg agcacgaatg
    43261 taagccggat aaggcgttta cgccgcatcc ggcagtcatg cgctacataa atttttcagg
    43321 agctaataat ggatcatcta cccatgccga aattcggacc gctggccgga ttgcgcgttg
    43381 tcttctccgg tatcgaaatc gccggaccgt ttgccggaca aatgttcgca gaatggggcg
    43441 cggaagttat ttggatcgag aacgtcgcct gggccgacac cattcgcgtt caaccgaact
    43501 acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt aaatattttc aaagatgaag
    43561 gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat cttcatcgaa gccagtaaag
    43621 gtccggcctt tgcccgtcgt ggcattaccg atgaagtact gtggcagcac aacccgaaac
    43681 tggttatcgc tcacctgtcc ggttttggtc agtacggcac cgaggagtac accaatcttc
    43741 cggcctataa caccatcgcc caggccttca gtggttacct gattcagaac ggtgatgttg
    43801 accagccaat gcctgccttc ccgtataccg ccgattactt ttctggcctg accgccacca
    43861 cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg taaaggcgaa agtatcgaca
    43921 tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt catgatggat tacttcaacg
    43981 gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc ctactacgcc ggttgcggtc
    44041 tgtataaatg tgccgacggc tacatcgtga tggaactggt gggcattacc caaattgaag
    44101 agtgctttaa agatattggc ctcgcacatc tactcggtac gccggaaatc ccggaaggca
    44161 ctcagcttat ccaccgtatc gaatgccctt acggcccact ggtagaagaa aaactcgatg
    44221 cctggctggc ggcacatacc atcgcagaag ttaaagaacg ctttgccgaa ctgaatatcg
    44281 cctgtgccaa agtactgacc gtaccggaac tggaaagcaa tccacagtat gttgcccgtg
    44341 aatcaatcac tcagtggcaa acgatggatg gtcgcacctg caaagggccg aacatcatgc
    44401 cgaaattcaa aaataacccc ggacaaatct ggcgcggaat gccctcacat ggcatggaca
    44461 cggctgccat tttgaaaaat atcggctaca gcgaaaacga cattcaggag ttggtcagca
    44521 aaggtctggc caaagttgag gactaaacgc gtggcgtcag tcggcttgcc tgactagcgc
    44581 ccgcccaata acaaacgaac aaaaatggat agaggtgcaa tggatatcat tggcggacaa
    44641 catctacgtc aaatgtggga cgatctggcg gacgtttacg gtcataaaac ggcgctgatt
    44701 tgtgaatcca gcggcggagt cgttaaccgg tatagttatc ttgagttaaa tcaggagatt
    44761 aaccgcacgg caaacctgtt ttatacgctg gggattcgca aaggcaacaa ggttgcacta
    44821 catctcgaca actgcccgga atttatcttt tgctggttcg ggctggcaaa aattggcgcg
    44881 attatggtgc cgattaacgc ccgcctgtta cgcgaggaaa gcgagtggat cttgcaaaat
    44941 agccaggcgt gcctgctggt gaccagtgcg caattttatc ctatgtatca acagattcag
    45001 caggaagatg ccactcaatt gcggcacatt tgcctgacag atgtggcact tcccgctgat
    45061 gatggagtga gttcatttac tcaactgaaa aatcaacaac ctgccacctt gtgctatgca
    45121 ccgccgctat cgactgacga tacggcggaa attctcttca cctccggcac cacctcccga
    45181 ccgaaaggtg tggtgattac ccattacaac ctgcgcttcg ctggatatta ctccgcctgg
    45241 cagtgtgcac tgcgtgacga tgacgtctac atgacggtaa tgcctgcgtt tcatatcgat
    45301 tgccagtgta ctgcggcgat ggcggcgttt tctgccgggg ccacctttgt gctggtcgag
    45361 aaatacagcg cccgcgcctt ctggggacag gcgcagaagt accgcgccac cattaccgaa
    45421 tgtattccga tgatgatccg tacgttgatg gtgcagccgc cttcagcgaa cgatcggcaa
    45481 caccgcctgc gggaagtgat gttttatctc aacttgtcgg agcaggaaaa agacgcattt
    45541 tgtgaacgct tcggcgttcg cttgctgacg tcttatggga tgacggaaac cattgtgggc
    45601 attatcggcg atcgccctag cgataaacga cgctggccgt cgattggtcg ggcggggttt
    45661 tgctacgaag cggagatccg cgacgatcac aatcgcccgc tcccggcagg tgagatcggt
    45721 gaaatctgta ttaaaggcgt accagggaaa accatcttca aagagtattt tctcaacccg
    45781 aaagccactg cgaaagtgct ggaagccgac ggctggctac ataccggcga taccggatac
    45841 cgcgacgaag aaggcttttt ttatttcgtc gatcgccgct gcaatatgat caaacgcggt
    45901 ggcgagaatg tctcctgcgt ggagctggaa aatatcatcg ccacccatcc gaaaattcag
    45961 gacatcgtgg ttgtgggtat taaagattcg attcgcgatg aagccatcaa agcatttgtg
    46021 gtgctgaatg aaggtgaaac attgagcgaa gaggaatttt ttcgcttctg cgaacaaaat
    46081 atggcgaaat ttaaagtgcc ctcttatctg gagatcagaa aagatctgcc acgtaattgc
    46141 tcggggaaaa taattagaaa gaatctgaaa taaatgaaac gtcagggaac taccctgcca
    46201 gctaataatc acgccctaaa acaatatgca ttttttgcag ggatgttgtc gtccctaaaa
    46261 aagcaaaaat ggagaaaagg aatgagtgaa tcattacatc tgacccgcaa tggatcaatt
    46321 ctggaaatta cccttgatcg cccaaaagcg aatgctattg atgcaaaaac cagctttgaa
    46381 atgggcgaag tatttctaaa tttccgtgac gatccgcaat tacgtgtcgc cattattacc
    46441 ggtgccggag agaaattctt ttccgcgggc tgggatttaa aagcggcagc agaaggcgaa
    46501 gcaccggatg ctgactttgg cccgggtggt tttgcgggat taaccgaaat tttcaatctc
    46561 gacaaaccag ttatcgcagc tgtgaacggc tacgcctttg gcggcggttt cgaactggcg
    46621 ctggcggcag attttattgt ttgtgccgat aacgccagct tcgccctgcc ggaagccaaa
    46681 ctgggcatcg ttcctgacag cggcggtgtg ctgcgtctgc cgaagatcct gccgcctacc
    46741 atcgtcaatg aaatggtgat gaccggcaga cgaatgggcg cagaagaggc gctgcgttgg
    46801 gggatagtca accgcgtggt tagccaggcg gaactgatgg ataacgcccg cgaactggct
    46861 cagcagctgg ttaacagcgc cccgctggcg attgcggcgc tgaaagagat ctaccgcacc
    46921 accagcgaaa tgccggtaga agaagcgtat cgctatattc gcagcggcgt gttgaaacac
    46981 tatccatcgg ttctgcattc ggaagatgcc attgaagggc cgctggcgtt tgccgagaag
    47041 cgcgatccgg tgtggaaagg acgttaacaa ccgtgagcta ttacgccttt gaggggttaa
    47101 ttccggtggt tcacccgacg gcgtttgtcc atcccagtgc cgtcttgatt ggcgatgtga
    47161 ttgtgggagc cggtgtctac atcggcccac tcgcctcact gcgtggtgac tacgggcggt
    47221 tgatcgtgca agcgggagcc aatattcagg atggctgcat tatgcatggc tactgcgaca
    47281 ctgacaccat cgttggggaa aacggccata tcgggcatgg agcgatcctg catggttgtg
    47341 tgattggtcg cgatgcattg gtcgggatga acagcgtaat tatggatggc gcggtcattg
    47401 gcgaagagag cattgttgcc gcaatgagct ttgtcaaagc aggctttagt ggcgagaaac
    47461 gccagttgtt gatgggtacg ccagcccgcg ctgtacgcag tgttagtgac gacgagttac
    47521 actggaaacg gttgaatacc aaagagtatc aggatcttgt cgggcgctgt catgcatcgt
    47581 tacatgaaac gcagccactg aggcaaatgg aggaaaatcg cccccgtttg caggggacga
    47641 cggatgtgac gcctaaacgg tgatcttgcc ggatgcgacc tggttgctgg ataagatgct
    47701 acaacatcgt atctggcaag caggacgccc ggtatcaacg agataaaatt aacgacgcat
    47761 actctttgac aacatcatct gccacttctg ctcgcggtta agttccgtga gcggaacggc
    47821 aggaatacgc ggtgtctttc ttacgccgac taatttttcc cgactgttat tgcgcaggcg
    47881 cgcgtaaatt tgctcatcga tatcgaccac tttaaccagt cgctgacact ggcatccccg
    47941 cccttccagc ttattaggga tcatcttaac ttcgcagctt atttctgtga cttccgacag
    48001 aatataagtc agggtattaa ccgccttgct gtgttcaata tcgaaatgaa tagagatctc
    48061 tcttgctatc acccaccgat tttcagccat catccattcg gcgattaaca agtagagtgg
    48121 tttttcaaca tatccttcac acatacgaac tccagtttca caaattaaaa gtctagctat
    48181 gactcgtttt gaagcaatta aacaaggcca tattaaaatt gtggatatat cgatagtatg
    48241 caacttcacc gtcgataagt gtgagctaaa tcctgcttat gtcatcaaaa atatcgattc
    48301 cccaaaagat ttacttaatg ggcaaaaaaa aacggtcctc atcagagaac cgtattagtt
    48361 attcgatcaa attagcggat gaaaaatatc cgccacgaca cgctattatt tgatctgtgc
    48421 gtgcatttcc tgcaccgaaa ttactttttc agtcacatcg gcattcagcg ccatcgcggt
    48481 agcgaaacca ccgttcaggg tggtgtcata atgcacttta tattgcagcg cactgcgacg
    48541 gatcacgcgg gagtcttcaa tcgcacgacg gcctgaggtg gtgttgatga tgtaggtata
    48601 ttcgccattc ttgatacggt cctgaatgtg cggacggcct tcatgcacct tgtttaccag
    48661 acgcggattg atacccgctt cgcccagcac aatcgccgtg ccgtgggtcg catccagctc
    48721 gaagccctgt ttcagcagtt ttgccgccag gtccaccacg cgttctttat cgccttcgcg
    48781 cacggaaagc agcgcacgac cgtgtttctt catggtggag ttgctgccca gctgcgcttt
    48841 ggcgaacgct tcagcgaagg tgcggcccac gcccatgact tccccggtag agcgcatttc
    48901 tggccctaac agcgggtcaa cgcctgggaa tttattgaac ggcagcacca cttctttcac
    48961 cgagtagtac ggcggaataa cttctttggt tacgccctgc tcagccagcg atttgccagc
    49021 catcacacgc gccgccactt ttgccagcgg tacgccggtg gctttggaga cgaacggaac
    49081 agtacgcgcc gcacgcgggt ttacttcaat caggtaaact tcgttgtttt tcaccgcaaa
    49141 ctgcacgttc atcagaccgc gcacctgcaa ttcgaaggcc agtttctgca cctgctggcg
    49201 catcacatcc tgaatttcct gacttaaggt gtacgctggc agcgaacatg cggagtcacc
    49261 ggagtgcaca cccgcctgct cgatgtgctc catgatgccg ccaatcagca ccatttcgcc
    49321 gtcgcagatg gcatccacgt caacttctac tgcgtcatca aggaaatgat ccagcaacac
    49381 tggcgcatcg ttagacacgc taaccgccgt ctggaagtag cgacgcaggt cagcttcgtc
    49441 atagacgatt tccatcgccc gaccgccgag aacgtaagac ggacgcacca ccagcgggta
    49501 gccaatctct tttgccttct caactgccat ttcaatagca gtaacggtgg cgttcgccgg
    49561 ttgtttcagt ttcagacggt caaccgcatg ctggaagcgt tcacggtctt ccgcacggtc
    49621 gatagcatcc gggctggtgc cgataaccgg tacgccagcg gcttccagtg cacgcgccag
    49681 tttcagcggg gtctgaccgc cgtactgaac gataacgcct ttcggcttct caatacgcac
    49741 gatttccagc acatcttcca gagttaccgg ctcgaagtag aggcgatcgg aagtgtcgta
    49801 gtcggtagag acggtttccg ggttacagtt aaccataatg gtttcgtaac cgtcttcacg
    49861 cagcgccagc gaggcgtgta cgcagcagta gtcgaattcg ataccctgac cgatacggtt
    49921 tggaccgccg ccaagcacca tgattttttc acggtcggta gacggattcg cttcgcactc
    49981 ttcttcataa gtggagtaca tgtaagcggt gtcggtggca aactctgccg cacaggtatc
    50041 cacgcgcttg tagaccgggt gcaggtcata ttggtcacgc agcttacgga tttccgcttc
    50101 gcgtacgccc gccagttttg ccaagcgcgc atcggcaaag cctttgcgtt tcagctggcg
    50161 caggaattca gcgttcaggc cagtgatgcc cacttccgcc actttctctt ccagacgcac
    50221 cagttcttca atctgtacca ggaaccagcg gtcaatgtta gtcaggttga agacgccgtc
    50281 cacagacagg cccgcgcgga aagcatcggc gatgtaccag atacgctctg cgcctgcgtc
    50341 tttcagttcg cgacggattt tggttaacgc ttccgggtca tccaggctca ctttcgggtc
    50401 gaagccagtt gcgcccactt ccaggccgcg cagcgctttt tgcagggatt cctgctgcgt
    50461 gcgaccaatc gccatcactt cgccaaccga tttcatctga gtggtcagac ggtcgttagc
    50521 accggcgaat ttttcgaagt tgaagcgagg aattttagta accacatagt cgatggacgg
    50581 ctcgaaggag gccggagtac gtccgccagt gatgtcgttc atcagttcgt ctagggtgta
    50641 acccaccgcc agtttcgccg ccactttagc aatcgggaaa ccggtcgctt tcgacgccag
    50701 cgccgaagaa cgggacacgc gtgggttcat ttcgataaca atcaggcgac cgtttttcgg
    50761 gttcaccgca aactggacat tggaaccacc ggtttcaacg ccgatttcac gcagcaccgc
    50821 catcgaggcg ttacgcatga tttgatattc tttgtcggtc agcgtttggg ctggcgcgac
    50881 agtgatggag tcaccggtgt ggatgcccat cgcatcaaag ttttcgatag agcagacgat
    50941 gatgcagttg tcgtttttat cacgcaccac ttccatctcg tactctttcc agccgatcag
    51001 cgactcatca atcagcaact ctttggtcgg agagagatcc agaccgcggg cgcaaatttc
    51061 ttcaaactct tcacggttat aagcgatacc gccgccgcta ccgcccatgg taaaggatgg
    51121 gcgaataatg cacgggaagc ccacgtcagc ggcaacagcc agcgcttctt ccatcgtgtg
    51181 tgcgataccg gaacgcgcgg tttccagacc aattttcttc atcgctacgt cgaaacgacg
    51241 gcggtcttct gctttatcaa tcgcatcggc agtggcacca atcatggtga caccgaactc
    51301 ttccaacacg ccctgacgtt ccagctccag cgcgcagttc agcgccgtct gaccgcccat
    51361 ggttggcagc accgcgtccg ggcgctcttt ttcaataatc ttgcgtacca cttcccagtg
    51421 aatcggctcg atgtaggtcg catcggccat ttccgggtcg gtcatgatgg tcgccgggtt
    51481 ggagttcacc agaatgacgc ggtaaccctc ttcgcgcagg gctttacatg cttgcgcgcc
    51541 agagtagtca aactcacacg cctgaccgat aacaatcggg cccgcaccca gaatcaggat
    51601 actttttata tctgtacgtt ttggcatggc tcttttactc ctgattactt agcggtttta
    51661 cggtactgct caattaactt gataaagtgg tcgaacaacg gcgcggcgtc gtgtggacca
    51721 gggctggctt cagggtgacc ctggaagctg aacgccggct tatcgatgcg atgaatgccc
    51781 tgtaacgtac cgtcgaacag ggatttatgc gtgacacgca ggtttgcagg taatgttgct
    51841 tcatccaccg caaaaccgtg gttctgggcg gtgatcatca ccacgttttt ctccacatct
    51901 ttaaccggat ggttgccgcc gtggtgacca aatttcattt tgacagtctt cgcaccgctc
    51961 gccagcgcca gcagctgatg accgagacag atgccgaata ccggaatatc ggtttcgagg
    52021 aacttctgga tggcggtaat ggcgtaatcg cacggggccg ggtcgccagg accgttggag
    52081 aggaagatgc cgtctggatt cattttcagc acatcttccg cagaagtttg cgccggaacg
    52141 atggtcaggc gacagcctct atccaccagc atccgcagga tgttgcgctt ggcaccaaaa
    52201 tcataagcca cgacgtggaa cggcagctcg tcttcttttt tcgcttctgg caggccgccg
    52261 gtcaacgtcc agctcccttg tgtccagcta taggcttctg cggtggtcac ttcttttgcc
    52321 agatccatgc cgttcagacc tgggaacgcg cgggcttttt ctaacgccag cgccgcatcc
    52381 gggttatcgc ctgcgataat gcagccattc tgtgcgcctt tctcgcgcag taaacgcgtc
    52441 agcttacggg tatcgatatc ggcaatcgcc acgatgttat ggcgcttcag gtaagaagag
    52501 aggtcttcgg tattacggaa gttgctggca atcagcggca ggtcgcgaat caccagacct
    52561 tgtgcatgta cctgagaaga ttcttcatcg gcgtcattag tgccgacatt gccaatatgg
    52621 ggataagtaa gagtaacgat ttgacgagaa taggaaggat cagtgaggat ttcttgataa
    52681 ccggtcattg aagtattgaa aacgacttcc ccaaccgccg aacctgttgc ccctatggcc
    52741 cgaccgtgaa actgggttcc gtcttccaga accaatagcg ctgacttaat caaaacaccc
    52801 tccagagaat actcactcac tttatttgca tattaattca cagtgatgac gtaaatcaat
    52861 gcaaatctgc ttaccgacga atttctggca aacggcggca ttctggagat atgggcgcta
    52921 aaagtcaact taatggcagg attttttata ttattttgtg gctcatgcat gttttacagg
    52981 cattaagcga caaaaacatc atctaacctg ttctggaaaa cgcttgcgca gcaagaagat
    53041 tgcatgaaat aaaaacgagc aggaaaaagt ggaccaaatg gtcaaaattt acattaaaac
    53101 aacaaaaata gaagacgaaa catgtaattt ttggcagtta tagaaaattt aaaagcataa
    53161 caccaaaaat aaaagggcta aaaattaaag ccctttataa ttaataaatt aaatgtgtta
    53221 cttttgcatc ataataaaca ttttgtggtt acaaattatt gagatcaagc acatctcgca
    53281 tatcaaaaag accactttcc ttaccactca accacaaagc cgatcttacc gcaccgttag
    53341 caaatgtcat gcggctggac gccttatggg tgatctccag acgctcgcca atatcggcaa
    53401 acatcgcggt atgttcgcca acgatgtcac ctgcacgcac ggtagcaaaa ccaatggtac
    53461 ctggcacacg ttcaccggta tggccttcac gactgtagac cgcgcaatct ttcagatctt
    53521 tatcaagggc gtgggcgatc gcctctccca ttgccagtgc ggtgcctgac ggcgcatcaa
    53581 ctttatgtct atgatgtgct tcaataattt cgatatcggt gtagtcaccc atcactttgg
    53641 ctgctttctc cagcagctta agcatgacgt taacgccaac gctaaaattg gcggcaaaga
    53701 caatcgcaat atcggcggcg gcgtcacgaa ttgcctgttt accggcttcg tcaaaccccg
    53761 tagtgccgat caccatccct ttgccatgct gccgacaaaa agcgagatgg ttcagcgtac
    53821 cttccggacg ggtaaaatcg ataaacacat caaaatcatc ttttatcgca tcaagactgc
    53881 tttgcacggt aacgcctgtt ttccctgcac cagccagctc accggcgtcg ctgcccagta
    53941 aagaagatcc ttcgcgctcc agcgccgcgc ccagctgcac gccttctaat gccagtgccg
    54001 cctgaatcaa ctggcggccc atacgccccc cggctcccgc gatggcaacg cggatgtttg
    54061 catcatgcat agctattctc ttttgttaat ttgcatagac cgttttcaga gtaaccagcg
    54121 catcgctacg ccgccagcca aaaacgctga taattagaaa ttaatgataa acagggaggg
    54181 atatcagtga aaggcatgag tcgatgaatg actgcatgcc gataacatgt gacaggttac
    54241 ggcaccagag ccagcacctc agccacccac tgttggaagc cttttacatt cagatccaat
    54301 gccacctgta cattggctgg tttgcccagg caaccgtcga tatcaaccac cgtcgtgcct
    54361 gaggtaaatt cgccttgagt ttctaccgcg acaaaacacg gcttgagggt gaataagtcc
    54421 gggcgcacca gccaggcgat ggcgcagaga tcgtgcattc gcagaccgct ttgcatactg
    54481 ccgctacggt agtggctaaa cagggcgtgg agcatttttc cggtgcgatt caactccggt
    54541 aaagtagcga gatagtcagg ggttaatatt gcctgattgg tgacatccag accgcacatg
    54601 acaatttcga tgccgctacg gaagacacag gcagccgctt ctggatcggc agcgatatta
    54661 aactcggcat ttggtgtaca gttgccgcga ccagcagaac cgcccatgat caccagacga
    54721 cgaatatacg gtttacattc cgggcattgt aaaagtaaca gcgcaatatt ggttaacggg
    54781 ccgatggcca ccagggtaac gggctctggt gcacgcatca gggcatcccg aatcgccaga
    54841 aacgccggta tcccgagtgg ctttcggttg tgctcaacaa agtcgtagcc agccattccc
    54901 gattcgccgt gcacagatgc tgcatcacgc ggtgcgcgta ccagcggcac agcggctccc
    54961 tgggcgagcg gaatctccgc attccagaaa tgcagcagtt gcagggcatt gcgggtagtt
    55021 ttctcaaccg agacattacc cgcgacggtg gtcatcagtt gcaggtcgag ttcgggggca
    55081 aaaatcgcgg cggcaatggc gacggcatcg tcaatgccgg ggtcagtatc gaggaagata
    55141 ggtaaacgca tgttttcttc ataaaaaatg ccggtaacaa gaccggcatt ttttatgaag
    55201 aaaacatgcc ggtaacaaga ccggcatttt cgcataactt aggctgctaa tgacttaatc
    55261 gacttcacga atatcgacac gcagctcttt cggcacttcg aaaacaatat tttcttcacg
    55321 gccttccagc ggaatggctt caccaccacc caactgctgc aaacgtgcca ccacattctg
    55381 caccagaata tccggagccg atgcgcccgc agtcacgccg acgcatttaa cctctttcac
    55441 ccactcttcc tggatatctt tcgcatcgtc aatcaaaaac gcgtgtttgc ccatacgctg
    55501 ggccagctcc gccagacggt tggagttgga ggagtttttc gaaccgacca ccaacacaac
    55561 ttccgcctgt tctgccaggg cgcgtaccgc ttcctgacgg ttagtcgtgg cgtagcagat
    55621 gtcatctttg cgcggaccga caattttcgg gaagcgttta cgcagcgcgt cgatcacatc
    55681 agacgtgtca tccaccgaca gcgtggtttg ggtcataaag gagagcttct cttcgttttt
    55741 gaccgtcagt ttccacacat cgtctggcga ttcgactaga tacattcccc cttcggggtt
    55801 gctgtactgc cccatcgtcc cttccacttc cgggtgcccg gcgtggccga tgagaataga
    55861 ttcttcgcca cggcgactgg cgcgggcaac ttccatatgc actttggtca ccagcggaca
    55921 ggtggcatca aacaccgtca aatcgcggct tttcgcttcg ttacgaaccg cctgagaaac
    55981 accgtgtgcg gagaaaatca ggatcgcgcc gtccggcact tcgctaatct gctcaataaa
    56041 gatagcccca cgctcgcgca agctatcgac cacgtagcgg ttatgcacca cttcgtgacg
    56101 gacatatatc ggtgcgccgt aaatggccag cgcgttttca acaatgctga tagcgcggtc
    56161 taccccggca caaaaaccac gcgggttggc caacaggatc tgcatgttac gcctccagtg
    56221 ccggatcgat ttccagcact tcaatatcaa aatgaacggt ctgcccggcc agcggatggt
    56281 tgaaatcaac ggtaatggag tcgccgttaa tttcgcggat cacaccaggc atctcactgc
    56341 catccattgc ggtaaaaagc atgattgcgc caatttctgg ctcgcctgca tccataaatt
    56401 cacggcggga gaagtactga atcaggtccg gtgacggcac gccaaacgcc gcatctggct
    56461 ccagcgagaa ggtggttttg tcgcccactt tcagccccag caggtgttgc tccagccctt
    56521 cagaaagaga agcatcgccc aggcggaaca gcgccggttt accgttgttg cgagtagact
    56581 cggcggtggt gccatcgtcg agttttagcg tgaagtgcac caggacggcg ctattgctct
    56641 gtacagattc agacatgcag gtttgctctt atttttgagg atttttgccg gatgcggcct
    56701 acgatttcgt agcaatctgt aggccgcatc cggcaggatt tattattgtt ttttcgcttt
    56761 ggaaggtaaa aacccttcca gcacgatcag tgccgcaccg acacaaatgg cagtatcggc
    56821 aaggttgaag gtggcgaagt gccagtcgcc gacgtagaag tcgatcatat cgacaacgaa
    56881 gccgtgccac aggcggtcga acaggttgcc cagcgcgccg ccaataatca gcgcgtaagc
    56941 gatattgttt agcttctgcg tggccttcga gcgatacatc atcactgcca ggagcacgct
    57001 aataccaatc gcaataccgg caaagaacca acgctgccag ccgccgctat cggcaaggaa
    57061 actaaacgcc gcgccatagt tacgcgcata atgcagatta agcgacggga acagcgggac
    57121 cgtatccccc agagcaaagt tctggaggat caggtatttg ctgcccagat cgataatcag
    57181 cacgactacc accagccaca gccagcgtag ccctgttgaa cagatcgatt gactcatcag
    57241 gcaaacttac gtttttcacc gtcaccggcg acgttgctga cacagcggcc gcagatttct
    57301 gcgtgttccg ccaccttgcc gacatcctgg gtgtagtgcc agcagcgtgg gcacttctca
    57361 ccttcggctt tactcaacgc gactttcagc cctttgagta cttcgctctg ctgagcatca
    57421 gcaggtgcgt cgttatagtc tgcaacggta gcgccggagg tcaacaggac aaatcgtaat
    57481 tcatcgccca gcgcggtcag tttcgccgcc agttccggtt ctgcatacaa ggttactgcc
    57541 gcttccagcg agccacccac tttcttgtcg gcacgcgctt gctcaatgac tttgttcact
    57601 tcgccacgca ctttcaacag ctcgtcccag aacgcatcgt tcattgcttc actgtctgcc
    57661 agaccaaaca ggccttcgta ccactcaccg gtgaagacgt atttttcacg ttcgcccggc
    57721 agatagcccc acacttcatc agcggtgaag gagaggattg gtgccatcca gcgcaccagc
    57781 gcttcagcga tgtgatacag cgcagtctgg cagctacgac gcgccacgct atccgctttg
    57841 gcggtgtact gacggtcttt gatgatgtcg aggtagaagg aacccatctc aacggagcag
    57901 aagcgcatca gacgctgtac cacttcgtgg aaatcgtatg cttcgtacgc cttgaggatg
    57961 tcttcctgtg ccgctttcgc acaacctacg gcccagcgat ccagtaccac catctcttcc
    58021 ggtttcacca tatcttttgc tggatcaaaa ccattcaggt ttgccagcag gaagcgcgcg
    58081 gtgttacgga tacgacgata gctatcggca gcacgtttca ggatctcgtc agaaacggcc
    58141 atttcaccgg tgtagtcggt tgatgccacc cacagacgca gaatatccgc gcccagtttg
    58201 ttcatcacat cctgcggcga aacggtgttg ccgatggatt tagacatctt gcgaccctga
    58261 ccatccacgg taaagccgtg ggtcagtacc tgacgatacg gcgctttgcc tttcatcgcg
    58321 gtggagatca ttagggaaga catgaaccaa ccacggtgct ggtcagaacc ttccagatac
    58381 atgtccgctg cgtgaccggc aaattccgga cgcacgtcaa caacagaaga gtgggtagat
    58441 ccggagtcaa accatacgtc gagagtatcc ggcactttca catactggtc agcttcgtcg
    58501 ccgaggatct ctttcgcatc gagatcccac cacgcctgga tgccgtcaac ttcaacgcgt
    58561 ttagccactt cttccatcag ttcgagggta cgcggatgca gctcttccgt gtctttgtgc
    58621 acgaacagtg acatcggtac gccccaggtg cgctgacggg agatacacca gtcaggacgg
    58681 ttagcaacca tcgactcgat acgcgcctgg ccccagtccg ggagccactg cacgcctttg
    58741 atctctttca gtgactgcgc acgcagacct ttctgatcca tgctgacgaa ccactgcggt
    58801 gtcgcgcgga agatgatcgg cgttttgtga cgccagcagc acggatagct gtgctgcatt
    58861 ttctcaacgt gcagcagcgc gcctttttcc tgcagcagcg caacgacgat gtcgttcgct
    58921 ttgaagacgt tcacgccatc cagcgtcgga taagtgcccg gcagataagt gccgtccggg
    58981 ccaaccgggt tagcggtttc caggccgtat ttctgaccga tcacatagtc gtccgggccg
    59041 tggccaggcg cggtgtgaac ggcaccggta ccggcatcca gggtaacgtg atcgccgagg
    59101 attgccggaa cgtcgaagcc cataaacgga tgggtaaagc gcagcagctc aagctctgca
    59161 ccttttaccg tgccgagaat ggtgtaatcg gtcacgccga tacgctgcat tacgctttca
    59221 accagatctt tcgccagaat cacggcctga ccgtcgatct gcaccagcgc atagtcgaaa
    59281 tcaggtgcaa tagagattgc gcggttcgca ggcagagtcc acggcgtagt ggtccagatc
    59341 accagcgaga ttgggccgtt aacgttgctt acgccaaatt tcgctttcag cgcatcctga
    59401 tcgaccgcct ggaaagcgac gtcaatggac ggagaagttt tgtcgtaata ctcaacttcc
    59461 gcttctgcca gtgcagaacg gcagtctacg caccagtgca ccggcttcgc gcctttgtgc
    59521 aggtgaccat tgccgatgat tttgcccagc gcgcggatga tgttggcttc agttttgaag
    59581 tccatagtca gatacgggtg cgaccagtcg cccagcacgc ccagacggat aaagtctttg
    59641 cgttgaccgt caacctgggt cgccgcgtat tcgcggcact tggcgcggaa ctcggcggcg
    59701 gtgaatttct cacccggctt accgtattct tgctctactt tcagctcgat cggcagaccg
    59761 tggcagtccc agccaggcac atacggcgag tcataaccgg aaagcccttt ggacttcacg
    59821 ataatgtctt tcagaatctt gttaaccgag tgaccaatat gaatgctgcc attcgcataa
    59881 ggagggccat catgcagaat gaaggttttt ttgccttttt tagccgcacg gatgatgccg
    59941 tacagatcat catcagtcca acgcgccagc attcccggtt cgcgcttggc gagatcgcca
    60001 cgcatcggga accctgtttc cggcaaattc agggttgatt tatagtcact catcagattc
    60061 tcggttccgt gtttcggttt gattacataa caggcttaag ccggttttgt tagcccaaaa
    60121 aattcgcggg cggttaattc atcacgcgca atctgcgctt tcagttcgtc cagcgacgca
    60181 aatcgctgct cattgcgtat ttttttacgc agcactactt gtatatggcg accgtaaagg
    60241 tccattgcaa catctaacaa atgcacttcc agctgctggc gaataccggc aaccgttggg
    60301 cgtgttccga tgtttgccac gccgggtaac ggcttttcac cgaggcccag cacttctacc
    60361 gcataaaccc ctttcaccgg ggaaacctga cggcgtagcg gtacattcgc cgtcgggaaa
    60421 cctatagtgc gccctaattc atcaccgtgg actacacgcc cggagatggc aaacgggtgc
    60481 cccagtaaac tttctgccag agccagattg tcatcagcca gcgcctgacg cacggcggtg
    60541 ctgctgatac gcacaccacc ttcgcaaaaa gtttgcgtac tggtgatatc gaagccgtat
    60601 tccatgccag ctttctgtaa taacaagaaa tcgccttcac gaccagcgcc aaagcggaaa
    60661 tcatcaccta cggcaagaaa ttttacgcgc aaatgcttca ccaaaagatc gctgatgaaa
    60721 ttttgcgcgg ttaacgccgc aaaacgcctg tcgaaacgca cgcacagcac gtaatcaacg
    60781 ccacactctg caaggtaacg cagtttttcc cgcagccggg tcagtcttgc cggggcttta
    60841 tcggtagcaa acagttccag tggttgaggt tcaaaaagca tcaccatcac cggtaagttg
    60901 cgcttgcgcc cttcttcctg caagccctgt aacagcgcgc gatgaccgcg atgcacgccg
    60961 tcgaaattac caatagtcag cacacaccct tcttgcgggg cctggctgag attatgtatg
    61021 ccgcgtatca gcttcatgtc tggctcaaaa cagtgaaaat cgtccgagta taccttgtac
    61081 agcggtcaag gttaaccggc gattgagtac cgaaatcaga aagaagtggc ttttcattgc
    61141 catggagcaa atcacgggaa gaaactgacc gcctgctgca atttttatcg cggaaaagct
    61201 gtattcacac cccgcaagct ggtagaatcc tgcgccatca ctacgtaacg agtgccggca
    61261 cattaacggc gcttatttgc acaaatccat tgacaaaaga aggctaaaag ggcatattcc
    61321 tcggcctttg aattgtccat atagaacaca tttgggagtt ggaccttggc taatatcaaa
    61381 tcagctaaga agcgcgccat tcagtctgaa aaggctcgta agcacaacgc aagccgtcgc
    61441 tctatgatgc gtactttcat caagaaagta tacgcagcta tcgaagctgg cgacaaagct
    61501 gctgcacaga aagcatttaa cgaaatgcaa ccgatcgtgg accgtcaggc tgctaaaggt
    61561 ctgatccaca aaaacaaagc tgcacgtcat aaggctaacc tgactgcaca gatcaacaaa
    61621 ctggcttaat cgccaatttg ctgaagcttt gtgaaaaagc ccgcgcaagc gggttttttt
    61681 atgcctgctg cttttgtgat gcgtgagaga acacactgcc ggttgccaga tagtcaaaag
    61741 agacatgccg ttgtactatc tgaactcttc gtggcttaag aatcgtagaa ccatcggctg
    61801 atacattctg aacaagaata tctactcaat ctccagactc ccccacaaag aatatggata
    61861 ttgtgataca cattgaggta ttaaacttaa gaccactcta tttttgaatt aaattcctat
    61921 atactttaac cattcataaa tatctcctca ttagacct
//
LOCUS       DAZSNM010000002        59890 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZSNM010000002 DAZSNM010000000
VERSION     DAZSNM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59890
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.002"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(82..486)
                     /locus_tag="ACHW42_000053"
                     /pseudo
     CDS             complement(82..486)
                     /locus_tag="ACHW42_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            complement(670..2088)
                     /gene="gltD"
                     /locus_tag="ACHW42_000054"
     CDS             complement(670..2088)
                     /gene="gltD"
                     /locus_tag="ACHW42_000054"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR8942059.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(2101..6561)
                     /gene="gltB"
                     /locus_tag="ACHW42_000055"
     CDS             complement(2101..6561)
                     /gene="gltB"
                     /locus_tag="ACHW42_000055"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR8942060.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            7236..8165
                     /locus_tag="ACHW42_000056"
     CDS             7236..8165
                     /locus_tag="ACHW42_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR8942061.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            8261..10597
                     /gene="arcB"
                     /locus_tag="ACHW42_000057"
     CDS             8261..10597
                     /gene="arcB"
                     /locus_tag="ACHW42_000057"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR8942062.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            10827..11480
                     /gene="elbB"
                     /locus_tag="ACHW42_000058"
     CDS             10827..11480
                     /gene="elbB"
                     /locus_tag="ACHW42_000058"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR8942063.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            11477..12204
                     /gene="mtgA"
                     /locus_tag="ACHW42_000059"
                     /pseudo
     CDS             11477..12204
                     /gene="mtgA"
                     /locus_tag="ACHW42_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(12201..12833)
                     /gene="yrbL"
                     /locus_tag="ACHW42_000060"
     CDS             complement(12201..12833)
                     /gene="yrbL"
                     /locus_tag="ACHW42_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR8942064.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(13046..13318)
                     /gene="npr"
                     /locus_tag="ACHW42_000061"
     CDS             complement(13046..13318)
                     /gene="npr"
                     /locus_tag="ACHW42_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR8942065.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            complement(13315..14169)
                     /gene="rapZ"
                     /locus_tag="ACHW42_000062"
     CDS             complement(13315..14169)
                     /gene="rapZ"
                     /locus_tag="ACHW42_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR8942066.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            complement(14215..14706)
                     /gene="ptsN"
                     /locus_tag="ACHW42_000063"
     CDS             complement(14215..14706)
                     /gene="ptsN"
                     /locus_tag="ACHW42_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR8942067.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            complement(14824..15111)
                     /gene="hpf"
                     /locus_tag="ACHW42_000064"
     CDS             complement(14824..15111)
                     /gene="hpf"
                     /locus_tag="ACHW42_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR8942068.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            complement(15134..16567)
                     /gene="rpoN"
                     /locus_tag="ACHW42_000065"
     CDS             complement(15134..16567)
                     /gene="rpoN"
                     /locus_tag="ACHW42_000065"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR8942069.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            complement(16615..17340)
                     /gene="lptB"
                     /locus_tag="ACHW42_000066"
     CDS             complement(16615..17340)
                     /gene="lptB"
                     /locus_tag="ACHW42_000066"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR8942070.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            complement(17347..17904)
                     /gene="lptA"
                     /locus_tag="ACHW42_000067"
     CDS             complement(17347..17904)
                     /gene="lptA"
                     /locus_tag="ACHW42_000067"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR8942071.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            complement(17873..18448)
                     /gene="lptC"
                     /locus_tag="ACHW42_000068"
     CDS             complement(17873..18448)
                     /gene="lptC"
                     /locus_tag="ACHW42_000068"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR8942072.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            complement(18445..19011)
                     /gene="kdsC"
                     /locus_tag="ACHW42_000069"
     CDS             complement(18445..19011)
                     /gene="kdsC"
                     /locus_tag="ACHW42_000069"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR8942073.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            complement(19032..20018)
                     /gene="kdsD"
                     /locus_tag="ACHW42_000070"
     CDS             complement(19032..20018)
                     /gene="kdsD"
                     /locus_tag="ACHW42_000070"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR8942074.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            complement(20032..20997)
                     /locus_tag="ACHW42_000071"
     CDS             complement(20032..20997)
                     /locus_tag="ACHW42_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR8942075.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            21207..22016
                     /gene="mlaF"
                     /locus_tag="ACHW42_000072"
     CDS             21207..22016
                     /gene="mlaF"
                     /locus_tag="ACHW42_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR8942076.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            22024..22806
                     /gene="mlaE"
                     /locus_tag="ACHW42_000073"
     CDS             22024..22806
                     /gene="mlaE"
                     /locus_tag="ACHW42_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR8942077.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            22811..23362
                     /gene="mlaD"
                     /locus_tag="ACHW42_000074"
     CDS             22811..23362
                     /gene="mlaD"
                     /locus_tag="ACHW42_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR8942078.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            23381..24016
                     /gene="mlaC"
                     /locus_tag="ACHW42_000075"
     CDS             23381..24016
                     /gene="mlaC"
                     /locus_tag="ACHW42_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR8942079.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            24016..24309
                     /gene="mlaB"
                     /locus_tag="ACHW42_000076"
     CDS             24016..24309
                     /gene="mlaB"
                     /locus_tag="ACHW42_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR8942080.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            24469..24723
                     /gene="ibaG"
                     /locus_tag="ACHW42_000077"
     CDS             24469..24723
                     /gene="ibaG"
                     /locus_tag="ACHW42_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR8942081.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            24778..26037
                     /gene="murA"
                     /locus_tag="ACHW42_000078"
     CDS             24778..26037
                     /gene="murA"
                     /locus_tag="ACHW42_000078"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR8942082.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(26085..26363)
                     /gene="sfsB"
                     /locus_tag="ACHW42_000079"
     CDS             complement(26085..26363)
                     /gene="sfsB"
                     /locus_tag="ACHW42_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR8942083.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(26590..27561)
                     /gene="ispB"
                     /locus_tag="ACHW42_000080"
     CDS             complement(26590..27561)
                     /gene="ispB"
                     /locus_tag="ACHW42_000080"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR8942084.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            27819..28130
                     /gene="rplU"
                     /locus_tag="ACHW42_000081"
     CDS             27819..28130
                     /gene="rplU"
                     /locus_tag="ACHW42_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR8942085.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            28151..28408
                     /gene="rpmA"
                     /locus_tag="ACHW42_000082"
     CDS             28151..28408
                     /gene="rpmA"
                     /locus_tag="ACHW42_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR8942086.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            28535..29500
                     /locus_tag="ACHW42_000083"
     CDS             28535..29500
                     /locus_tag="ACHW42_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR8942087.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            29516..30688
                     /gene="cgtA"
                     /locus_tag="ACHW42_000084"
     CDS             29516..30688
                     /gene="cgtA"
                     /locus_tag="ACHW42_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR8942088.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(30709..30810)
                     /locus_tag="ACHW42_000085"
                     /pseudo
     CDS             complement(30709..30810)
                     /locus_tag="ACHW42_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(30874..32307)
                     /gene="dacB"
                     /locus_tag="ACHW42_000086"
     CDS             complement(30874..32307)
                     /gene="dacB"
                     /locus_tag="ACHW42_000086"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR8942089.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            32555..33031
                     /gene="greA"
                     /locus_tag="ACHW42_000087"
     CDS             32555..33031
                     /gene="greA"
                     /locus_tag="ACHW42_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR8942090.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            complement(33187..33480)
                     /gene="yhbY"
                     /locus_tag="ACHW42_000088"
     CDS             complement(33187..33480)
                     /gene="yhbY"
                     /locus_tag="ACHW42_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR8942091.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            33606..34235
                     /gene="rlmE"
                     /locus_tag="ACHW42_000089"
     CDS             33606..34235
                     /gene="rlmE"
                     /locus_tag="ACHW42_000089"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR8942092.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWLKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            34335..36269
                     /gene="ftsH"
                     /locus_tag="ACHW42_000090"
     CDS             34335..36269
                     /gene="ftsH"
                     /locus_tag="ACHW42_000090"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR8942093.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            36359..37207
                     /gene="folP"
                     /locus_tag="ACHW42_000091"
     CDS             36359..37207
                     /gene="folP"
                     /locus_tag="ACHW42_000091"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR8942094.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            37200..38537
                     /gene="glmM"
                     /locus_tag="ACHW42_000092"
     CDS             37200..38537
                     /gene="glmM"
                     /locus_tag="ACHW42_000092"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR8942095.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            38765..39097
                     /gene="secG"
                     /locus_tag="ACHW42_000093"
     CDS             38765..39097
                     /gene="secG"
                     /locus_tag="ACHW42_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR8942096.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            39112..39198
                     /locus_tag="ACHW42_000094"
     tRNA            39112..39198
                     /locus_tag="ACHW42_000094"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39146..39148,aa:Leu,seq:gag)
     gene            39657..41283
                     /locus_tag="ACHW42_000095"
                     /pseudo
     CDS             39657..41283
                     /locus_tag="ACHW42_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(41291..42634)
                     /gene="argG"
                     /locus_tag="ACHW42_000096"
     CDS             complement(41291..42634)
                     /gene="argG"
                     /locus_tag="ACHW42_000096"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR8942097.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            42982..43058
                     /locus_tag="ACHW42_000097"
     tRNA            42982..43058
                     /locus_tag="ACHW42_000097"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43016..43018,aa:Met,seq:cat)
     gene            43265..43717
                     /gene="rimP"
                     /locus_tag="ACHW42_000098"
     CDS             43265..43717
                     /gene="rimP"
                     /locus_tag="ACHW42_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR8942098.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            43745..45232
                     /gene="nusA"
                     /locus_tag="ACHW42_000099"
     CDS             43745..45232
                     /gene="nusA"
                     /locus_tag="ACHW42_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR8942099.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            45257..47905
                     /gene="infB"
                     /locus_tag="ACHW42_000100"
     CDS             45257..47905
                     /gene="infB"
                     /locus_tag="ACHW42_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR8942100.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            48069..48470
                     /gene="rbfA"
                     /locus_tag="ACHW42_000101"
     CDS             48069..48470
                     /gene="rbfA"
                     /locus_tag="ACHW42_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR8942101.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            48470..49414
                     /gene="truB"
                     /locus_tag="ACHW42_000102"
     CDS             48470..49414
                     /gene="truB"
                     /locus_tag="ACHW42_000102"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR8942102.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            49563..49832
                     /gene="rpsO"
                     /locus_tag="ACHW42_000103"
     CDS             49563..49832
                     /gene="rpsO"
                     /locus_tag="ACHW42_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR8942103.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            50079..52214
                     /gene="pnp"
                     /locus_tag="ACHW42_000104"
     CDS             50079..52214
                     /gene="pnp"
                     /locus_tag="ACHW42_000104"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR8942104.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            52323..53207
                     /gene="nlpI"
                     /locus_tag="ACHW42_000105"
     CDS             52323..53207
                     /gene="nlpI"
                     /locus_tag="ACHW42_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR8942105.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            53314..53394
                     /gene="yrbN"
                     /locus_tag="ACHW42_000106"
     CDS             53314..53394
                     /gene="yrbN"
                     /locus_tag="ACHW42_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR8942106.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            53387..55276
                     /gene="deaD"
                     /locus_tag="ACHW42_000107"
     CDS             53387..55276
                     /gene="deaD"
                     /locus_tag="ACHW42_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR8942107.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            55430..56674
                     /gene="mtr"
                     /locus_tag="ACHW42_000108"
     CDS             55430..56674
                     /gene="mtr"
                     /locus_tag="ACHW42_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR8942108.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(56792..57799)
                     /locus_tag="ACHW42_000109"
     CDS             complement(56792..57799)
                     /locus_tag="ACHW42_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR8942109.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(57880..58758)
                     /locus_tag="ACHW42_000110"
     CDS             complement(57880..58758)
                     /locus_tag="ACHW42_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR8942110.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            complement(58767..59762)
                     /locus_tag="ACHW42_000111"
     CDS             complement(58767..59762)
                     /locus_tag="ACHW42_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR8942111.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
BASE COUNT        14240 a        14832 c        16214 g        14604 t
ORIGIN      
        1 cttcgtttat ttgcatgaat atacaaaccg aaaataggag atgcaataca aaagctgtgg
       61 aaatatcaaa cagcctctca atttggcaga cagcgtctgc caaatctttg ccgatgggaa
      121 ttctgcagat ggtgtctgcg gaattcccaa tggtgttaat tcgccagaca ctgtctggcg
      181 aatttcaaca tgttgaaaaa agaggtaaaa aagcctcatt tggcgtaagt tttttgcaga
      241 gaccccacgt ttataccgtc gacttaaatc cttcgatagc cgatcaatca actgtgcacc
      301 ataagctgct ctggcctcac caccttgttc aaattcgaca atccgtcggc caatttccca
      361 gtacgtcgcg gtcatcagcg cgttaacgct acgtaccgtt tccgttcggg cgctatcgac
      421 cagatgaata atgccgttgt ggatttgctg gtagcctgtt gaggttctct ctgagagcga
      481 ttccattatc tgtcccttta tatggcgctg cgttgaataa tggaatcagc tatacgccgg
      541 gcatctaccg gtaacaatcc agatgagcgt attacattgg ttttcttcag attatgcgag
      601 gcgatttcca gaaagaaagg agaatttcag cgccgactct tttctgagga ggcggcgctg
      661 ggacagagct taaacttcca gccagttcat aatgccgtct gccgctttgc gaccttcggc
      721 gatggcggta acgaccagat cggaaccacg gacgatatcg ccgccagcaa agattttcgg
      781 gttgctggtc tggaaagcgt tgtcgttgcc ttccggagcg atgatgcggc cttgtgaatc
      841 cagctcgacg ctgtgttttg ccagccattc catgttgtgt ggacggaaac caaacgccat
      901 gatcaccgca tctgccggaa cgatatgttc ggaacctgca acgatctccg cgcggcgacg
      961 gcctttggca tccggttcgc ccatttcggt acgtaccatt tttacgccgc tgactttgcc
     1021 gttaccgttc acttcaatcc ccagtggctg gacgttgaat ttgaactcta cgccttcttc
     1081 ccgcgcgttt ttcacttcgc ggcgggaacc cggcatgttc tcttcatcac gacgataggc
     1141 acaggtaacg tgctttgccc cctggcgtac agacgtgcgc acgcagtcca tcgcagtgtc
     1201 gccaccgcca agtactacca cgcgtttgcc ttccatgctg acgaacggtt cgtcgcgggt
     1261 ttcaccaaag cccattaact gtttggtgtt ggcgataagg aacggcagcg ctgcgtacac
     1321 gccatcggcg tcttcgtttt ccagcccgcc gcgcattgac tgataagtcc caacgccaag
     1381 gaacacggca tcgtaatcac tcagcagatc gtccagctgt acgtcgcggc ccacttcggt
     1441 attgagtttg aattcaatac ccatgccggt gaagatttca cggcgacgcg tcattacctc
     1501 tttttccagc ttgaaggccg gaataccgaa ggtcagcagc ccgccaattt ctggatgacg
     1561 gtcgaagaca acggctttta cgccgttacg cgtcaggaca tccgcacacg ccagacctgc
     1621 cgggcctgca ccgataatcg ccactttttt accggtttgc ttcacgccgg acatatccgg
     1681 acgccagccc atttcgaacg ctttatcatt gatataacgc tcaatgttgc cgatggtcac
     1741 cgcaccgaac tcatcgttca gggtgcagga accttcgcac agacggtctt gcgggcagac
     1801 acgtccgcat acttccggca gtgtgttggt ctgatgcgac agttctgccg cttcaaaaat
     1861 acgcccctcg ttggcgagct tcagccagtt tgggatgtag ttgtgtaccg ggcatttcca
     1921 ctcgcagtat gggttgccgc acgacagaca gcggtcagcc tgcgctttgg cctggccttc
     1981 ggaaaacggc tcgtaaattt caacaaactc aattttacgg atcttcagcg gtttctttgg
     2041 cggatcaacg cgctgcaggt cgataaattg ataaacattc tgactcattg ttgctacccc
     2101 ttactgcgcc tgcacgcgca actctgctgc gctacgacta cggtgaccca gcagtgcttt
     2161 tacatcactg gacttcggtt taaccagcgc gaatttcgtg gcgaaggttg accagttcgc
     2221 cagaatctct tcaccgcgct gagaaccggt atgctgaaca tgctcggtga taagaccgcg
     2281 cagatgctct tcatggatcg ccagatcgtc gacgcttaag acttcgacca gttccgggtt
     2341 aacgcgtttg cggaaatcac cgctttcatc gagaacgtaa gcgaagccgc cggtcatgcc
     2401 cgcgccgaag ttaacgccgg ttttacccag aatacagaca ataccgcccg tcatatattc
     2461 acagccgttg tcgccaatgc cttctaccac ggtgatagca ccggagttcc gcacgccgaa
     2521 acgttcaccc gcgcggcctg cggcatacag acgaccaccg gtcgcgccat acaggcaggt
     2581 gttgccgata atgcttgctt catggctgcg gaaggcggaa ccaaccggag gacgaatggc
     2641 gattaagccg cccgccatgc ctttaccgac atagtcgttg gcatccccgg tcaggtacag
     2701 ttccacgccg cccgcgttcc acacaccgaa actctggcct gcggtgccgt tgaagtacgc
     2761 tttgatagga tcggctgcca gcccctgatc gccgtgcgtc tgggcgatat agcctgaaag
     2821 cgacgcgccg acagaacggt cggtgttgcg gatatcgaac cagaaggttt tgctctggcg
     2881 ttcatcgaca aacggtttcg cctgttgcag caactgcgcg ttcagtaggc cgttatcaaa
     2941 cggcgggttg ttttcagtgc agtagagtga cttacctgca tgtggctcgg cagtctccag
     3001 cagcttcgac agcgccagtt tctgctgttt ggcggtgaaa ccgtccagct ctttcagcag
     3061 gtcggtgcga ccaatcagat ccaccagacg cgttactccc agctgtgcca tcagctcgcg
     3121 ggtttcacgg gcgataaact caaagtaatt agtcaccttg aacggcaggc cgtgatagtg
     3181 attcttacgc agtttgtcat cctgagttgc tacacccgtg gcgcagttgt tcaggtggca
     3241 aatacgcaga tatttacagc cgagcgccac cattgggcca gtgccgaagc cgaagctttc
     3301 tgcgccgaga atcgccgctt tgatgatatc gacacccgtt ttcaggccgc catcaacctg
     3361 caaacgaatt ttatgacgca agccgttggc aaccagcgcc tgttgggttt ccaccagacc
     3421 gagttcccac gggcagcctg catatttcac tgacgacagc gggcttgcgc cggtgccgcc
     3481 gtcatagccc gcgatggtga tcaagtccgc ataagctttc gccacgccag tcgcgatggt
     3541 gccaacgcct ggttcggaca ccagcttaac ggagatcatc gctttcgggt taacctgctt
     3601 gaggtcgaaa ataagctgcg ctaagtcttc gatagagtag atatcgtggt gcggcggcgg
     3661 ggagatcagc gtcactccgg gcaccgaata gcgcagtttg gcgatgtaag gagtgacttt
     3721 gtcgcccggc aactgaccac cttcgcccgg cttcgcgccc tgggcgactt taatctgaat
     3781 gacgtcggca ttgaccagat acgccggagt aacgccgaag cgaccagaag ccacctgctt
     3841 gatgcgcgac actttgttag tgccatagcg cgccgggtct tcgccgcctt caccggagtt
     3901 cgagttaccg ccgatgctat tcatcgcttc cgccagcgcc tcgtgggctt ccgggcttaa
     3961 cgcgccgata gacatcgcgg cggtatcaaa gcgtttaaac agttcgcttg ccggttcaac
     4021 atcagcaatg ttgaccgcgt tttcacccgg cgtaattgcc agcagatcgc gcagcgtggt
     4081 tgccggacgc tcattaacca gcttcgcgta ttcctgatag tcgctgtact cgccgctttg
     4141 taccgcctgt tgcagcgtgc gcaccacgtc cgggttgtag gcgtggtatt cgccgccgtg
     4201 gacgtatttc agcaggccgc cctggctaat cggcttgcgc gccagccagg cacgtttcga
     4261 cagattcagc agatcctgct ggaagtcttc aaagcttgcc ccaccaatgc ggctgaccgc
     4321 gccctggaag cacaggccca ctacatcatc gtgtagaccg accgcttcaa acagtttcga
     4381 gcagcggtaa gaggcgatgg tggagatgcc cattttggac atgattttgt acaagccttt
     4441 gttgatgccg ttacggtagt tgagcatcac ggtacgataa tctttggcaa tcgcgtgggt
     4501 gtctaccagg cggcccagcg tttcataggc aaggtacgga taaatagccg tcgcgccgaa
     4561 acccagcaac acggcgaagt ggtgcggatc gcgggcgctg gcggtttcga cgatgatgtt
     4621 ggcatcgcaa cgcaggcttt gatcgaccag acgggtctgg atcgcgccaa ccgccatcgg
     4681 agccggaacc ggcaggcgat ctttagcgat attccggtcg gagagcacca gcaacacggt
     4741 gccgctacgt accatttttt cggctttgtc gcatagctct ttgactgtcg cttcgagcgt
     4801 ggttttagtg acgtcaaagg tgatatccag cgtatctgcg cggtagtgct cctctttcat
     4861 cgtcgtgagc tgtttgaaat cggagtagag cagaatcggc gatttaaagc ttaaacggtg
     4921 cgcctggccc tctgcttcgc aaaagacgtt catttcacga ccgatactgg tggcgagcga
     4981 cataacatgc gcttcacgca gcgggtcgat tggcgggtta gtcacctggg caaactgctg
     5041 gcggaagtag tcgtaaataa tgcgcggctg actggagagc acggcgaatg gggtatcatc
     5101 gcccatcgaa ccgaccgctt cctgaccgtt ttcgcccagt acgcgaatta cggagtccag
     5161 ctcttccgcg ctgtagttaa actgtttctg gtagctggca agcgtgtcgt cgtccagttc
     5221 gcggctaccc acttcttcat cgggcagatc ttcaaacggt accagtcggc ggacgttttt
     5281 ctccatccac tctttataag ggtggcggct tttcagatcg tcatcggttt ctgccgagtg
     5341 cagaatacgc ccactgcgag tgtcgatcac catcagttcg cctggcccga cgcggccttt
     5401 ttcgaccact tcgtcaggct ggtaatccca gataccgact tcagaggcgc aggtgatgag
     5461 cttgtctttg gtaatgacgt agcgtgccgg acgcagaccg ttacggtcga ggttacaggc
     5521 agcgaagcga ccatcggaca tcacgatgcc cgccggacca tcccacggct ccatatgcat
     5581 ggagttaaag tcaaagaagg cacgcagttc cgggtccata tccgggttgt tctgccaggc
     5641 gggtggcact aacaggcgca tggcgcggat gatatccatc ccgcctgcca gcagcagttc
     5701 cagcatgtta tccatggaac tggagtcaga accggtttcg ttgacgaatg gcgcggcgtc
     5761 gtgcaggtca gggatcagcg gcgtctggaa tttataggta cgcgcacgcg cccattggcg
     5821 gttaccggtg atggtgttga tttcaccgtt atgcgccaga tagcggaacg gttgcgccag
     5881 cggccagcgc ggtacggtgt tagtggagaa gcgctggtgg aacaggcaaa tggccgattc
     5941 cagacgcagg tccgcaagat ccagataaaa gcgcggcaga tccgccggca tacacagacc
     6001 tttatagatg ttcaccagat tcgacaggct acagacgtag aagtctttgt cggcttcgag
     6061 acgcttttca atgcggcggc gggcgataaa cagacggcgc tccatatcgc gtggacgcca
     6121 gcctgccggg gcgttcacaa aaatttgctc aatgcgtggc agagaggaga gggcgatttc
     6181 acccagcacg ccttcgttag tggggacatc acgccagccc acaatcgaca aggtttcgcg
     6241 ttgcagttct tcttcaacga tgcggcgtgc ggcagcggcg agttcaggat ctttattcag
     6301 gaagagcatc ccgacagcgt agttttttgc taaacgccag ccgcgctcct gcgcaacgat
     6361 gcgaaaaaag cgatccggtt tttgtaacag caagccgcaa ccgtcgccgg ttttaccatc
     6421 ggcgagaatc gcgccacggt gctgcatgcg ggccagtgcg tgtattgcag tacgcactac
     6481 cttgtggcta ggttcgcctt ctatgtgggc gatcaggccg aaaccacagt tatccctctc
     6541 aagggattta tcgtacaaca tatcgtgaac ctcccccagg ctctgcggga accccacttc
     6601 cgaaccgctg cgcacgggca cagaaagagc gtggcgacgg ggtttgcgtg tcatacgcgc
     6661 gcctcgcatt cgcccccttg ttgtcctttt cgcatcggtt aatacggtga agaggggatc
     6721 tcaattactg cataaatatg atgagcaggc tgctcatcca gaaagcttcc agcggatttc
     6781 caacttatcg ggaaagctga tacaggtcaa atggcaagct tattggtaca gaaatgtgct
     6841 ttagcgcaaa attctttatt aaatagctga aattaaaata gattttatct ttaaggctaa
     6901 aaggagagtc gttagagaaa cagtcaattc ctgactgtca taaaggcgtg ttaaccctta
     6961 tcctgccttg aactgacgag aaaaccagca ttttatactg ccttaattgg taatgacgtt
     7021 tcatgctact gtttttgcct aaaatccata aggagataaa aaaatctgac acaagataag
     7081 caaaagtgat ttatgtgatt gtgcatgaaa tttcggtaat tttgattgat tatgcagttg
     7141 ataaaagcag gccgggacga ttgatccagg tcatcgccga attgagctaa aaatggcagg
     7201 ctttggcctc ttttcaaggc gcggtctatc agattatgca gttacagaaa ttagtcaata
     7261 tgtttggtgg tgatctcacc cgtcgttatg ggcaaaaggt gcataagctg acgctgcacg
     7321 gcggttttag ctgccctaac cgtgacggta ccatcgggcg tggtggctgc acattctgta
     7381 atgttgcctc gtttgccgat gaagcgcagc agcatcgttc cattgccgag caactggcgc
     7441 accaggcgaa tttagttaac cgcgctaaac gctatctggc ctactttcag gcgtatacca
     7501 gcacctttgc ggaagttcag gtgctgcgtt ctatgtatca gcaggcggtg agccaggcca
     7561 atattgtcgg tttgtgtgtt ggtacccgcc cggactgcgt gccggatgcg gtgctggatc
     7621 tgctttgcga atataaggac cagggttacg aagtgtggct ggagctgggg ctacaaaccg
     7681 cccacgacaa aacactgcat cgcatcaacc gcggccatga ttttgcctgt tatcagcgta
     7741 caacccagct ggcacgtcag cgcgggctga aggtgtgttc ccacttaatt gtcggcctgc
     7801 ctggcgaagg gcaggctgaa tgtttgcaaa cgctggagcg agtggttgaa accagcgtgg
     7861 acggcataaa gctgcatccg ctgcatattg tgaaaggcag cattatggcg aaagcctggg
     7921 aagcggggcg tttgaacggt attgaactgg aggattatac gctcactgcc ggagaaatga
     7981 ttcgccatac gccaccggaa gtgatctacc accgcatttc cgccagcgcc cgtcgcccga
     8041 cgctgcttgc tccgctgtgg tgcgaaaatc gatggacggg aatggtcgag ctggatcgct
     8101 atctgaatga acatggcgta cagggatcgg cgctgggacg tccgtggcta cctccaacgg
     8161 agtaggtcgt tgaggggaat tccgcatttc tcacacaatt tataacgtaa ctgtcagaat
     8221 tgggtattat tggggcaggt tgtcgtgaag gaattcccta atgaagcaaa ttcgtctgct
     8281 ggcgcagtat tatgttgacc tgatgatgaa gttaggtctg gtgcgcttct caatgttgct
     8341 ggcgctggcc ctcgtcgttc ttgccattgt ggtacaaatg gcggtaacca tggtgctgca
     8401 tggtcaggtc gaaagcattg atgttattcg ttctatcttc tttggtttgc tgattacgcc
     8461 gtgggcggtc tactttctat cggtggtcgt cgagcaactg gaggagtcac gacaacgtct
     8521 gtcacggctg gtgcaaaaac tggaggagat gcgcgagcgc gatttgagcc tcaacgttca
     8581 gttaaaagat aatattgccc agctaaatca ggaaattgcc gttcgtgaaa aagcggaagc
     8641 agaactgcag gaaaccttcg gccaactgaa aattgaaatc aaagagcgcg aagagacaca
     8701 aattcagctc gagcagcaat cctcattctt acgttccttc cttgatgctt cacccgactt
     8761 ggttttttat cgtaacgaag ataaagagtt ttccggctgt aaccgcgcga tggagctgct
     8821 gaccggaaaa agcgaaaaac aactggttca cctgaaacct gctgatgttt actcaccgga
     8881 agccgccgca aaagtcattg aaaccgatga aaaagtgttc cgtcataatg tgtcactgac
     8941 ctatgaacag tggctggatt acccggacgg gcgcaaagcc tgctttgaaa tccgtaaagt
     9001 gccgtactac gaccgcgtgg gtaaacgtca cggtttgatg ggctttggtc gcgacattac
     9061 cgagcgtaag cggtatcagg atgcgcttga acgcgccagc cgcgacaaaa cgacgtttat
     9121 ttccaccatc agtcacgaat tgcgtacacc gctgaacggt atcgtcggcc tgagccgcat
     9181 tctgctggat accgaactca ccgccgagca ggaaaaatat ctcaaaacta tccatgtttc
     9241 ggccgtcacg ctggggaata tctttaacga tattatcgac atggataaga tggaacggcg
     9301 caaggtccag cttgataatc agccggttga tttcaccagc ttccttgccg atctggaaaa
     9361 tctctccgca ttgcaggcgc aacaaaaagg attgcgcttt aacctggagc ctacgctgcc
     9421 attaccgcat caggtcatta ccgacgggac gcgtttacgg cagatcctgt ggaacctcat
     9481 cagtaacgcc gtcaaattca cccagcaagg ccaggttacc gtgcgcgtgc gctacgatga
     9541 aggcgatatg ctgcattttg aagtggaaga ttctggtatc ggcattccgc aggatgagct
     9601 ggataaaatc ttcgccatgt attaccaggt gaaagacagt catggcggta aacctgccac
     9661 cggcaccggt attggtctgg ccgtttctcg tcgtctggcg aaaaatatgg gcggcgatat
     9721 tacggttacc agtgaacagg gcaaaggttc aacctttacg ttgacgatcc acgcaccgtc
     9781 ggtggcagaa gaggtcgatg atgcgtttga tgaagacgat atgcctttac cggcgctgaa
     9841 tgtgctgctg gtggaagaca ttgaactgaa cgtgattgtc gcgcgttctg tgctggaaaa
     9901 attaggtaac agcgttgatg tcgccatgac cggcaaggcg gcgctggaga tgtttaaacc
     9961 gggcgaatac gacctggtgt tgctggatat tcagttgcca gatatgaccg ggctggatat
    10021 ctctcgtgaa ctgacgaagc gttatccgcg cgaggattta ccgccgctgg tggccttaac
    10081 cgctaacgtg ctgaaagaca aacaagagta cctcaatgct ggaatggatg atgtgctgag
    10141 taagccgctt tcggttccgg cgctaaccgc gatgatcaag aaattctggg atacccagga
    10201 tgatgaggag agtacggtga cgacagaaga gaacagtaaa tcagaagcat tgctcgatat
    10261 tcccatgctg gaacagtatc tcgaacttgt aggaccgaag ctgatcaccg acgggttagc
    10321 ggtgtttgag aagatgatgc cgggctatgt cagcgtgctg gagtcgaatc tgacggcgca
    10381 ggataaaaaa ggcattgttg aggaaggaca taaaattaaa ggtgcggcgg ggtcagtggg
    10441 gttacgccat ctgcaacagc tgggtcagca aattcagtct cctgaccttc ccgcctggga
    10501 agataacgtc ggtgaatgga ttgaagagat gaaagaagag tggcgtcacg acgtagaagt
    10561 gctgaaagcg tgggtggcaa aagccactaa aaaatgaccc cggctagacc ggggtgcgcg
    10621 aatactgcgc caacaccagg gaaatcttgg ctgcgccgta aattattatg atgagttaca
    10681 agggcacagc actgtttttc aggccgcacg cagttaagat agcaaaactt aaatgatttg
    10741 ttacatgaat cagttaaatg tgtgatgcgt accattttta ttaaaaaaat taatgtgttt
    10801 cagtcaattg cgaaaggatg atcacaatga agaaaattgg cgtaattctg agcggatgcg
    10861 gcgtctatga cggttctgaa attcatgaag cggtgttgac gttgttagct atttcacgca
    10921 gcggtgcgca ggcggtctgc tttgcaccgg ataagcagca ggttgatgtt atcaaccatt
    10981 taactggcga agcgatgacg gaaacgcgca atgtgctgat tgaagcggca cgaataacgc
    11041 gcggtgaaat ccgtcctctg gcccaggccg atgccgctga actggatgcg ttgattgtgc
    11101 cgggggggtt tggcgcggcg aagaatttaa gcaattttgc cagtcttggt agcgaatgca
    11161 ccgttgaccg tgaattaaag gcgctggcac aagcgatgca tcaggccgga aaaccgcttg
    11221 gttttatgtg tattgccccg gcgatgctgc cgaaaatttt cgatttcccg ctgcgtttga
    11281 ccatcggtac tgatatcgat accgcagaag tgctggaaga gatgggcgcg gagcatgtgc
    11341 cgtgtcctgt cgatgatatc gtggttgatg aagacaataa gattgtcacc accccagcat
    11401 atatgctggc gcagaacatt gcagaagcgg cgagcggcat tgataagctg gtttcccgcg
    11461 tgctggttct ggctgaatga gtaaaagccg cttaacggtg tttagtttcg ttcgccgttt
    11521 tcttttgcgg ttaatggttg tcctcgccgt tttctggggc gggggcatcg cgttgtttag
    11581 cgttgcgcct gttcccttct cagcggtaat ggtcgagcga caggtcagcg cctggctgca
    11641 tggcaatttt cgttacgtgg cacattctga ctgggtcagt atggatcaaa tctcgccgtg
    11701 gatgggactg gcggtgattg ccgcagaaga tcagaaattt cctgagcact ggggctttga
    11761 tgtcgcttcc attgagaaag ccctggcgca caacgagcgc aatgaaaacc gtattcgcgg
    11821 tgcttcaacg atttctcagc agacggccaa aatctctttt tatgggatgg gcgtagctgg
    11881 gtacgaaaag ggctggaagc tggattaacg ctggggatag aaacagtctg gagcaaaaag
    11941 cgtatcctga cggtttacct gaatatcgcc gaatttggcg acggtgtgtt tggcgtcgaa
    12001 gctgcggcac aacgttattt ccacaaaccc gcgagcaaac ttacccggtc ggaagctgca
    12061 ttactggcag ctgtattacc taatccactt cgtttcaaag tctcctcgcc atcgggctac
    12121 gtgcgtagcc gtcaggcgtg gattttacgg cagatgtatc agttaggcgg tgagccgttt
    12181 atgcaacaac accagctgga ttaagcttcg cgcgaagtga gggccagcgt tttgctctca
    12241 cgaggttgca gatctttctg cagggcaata gccagcgccg gttgggcgat aaatcgtttc
    12301 cataaccgct cttgcttacg cagacaacac cacttcgacc aggtcgccag tggaatcaat
    12361 gtgctttcac caatgttgtc gcagacgacc gggataacct ctgactcact gatgcggtga
    12421 cagaggatat tctgcggctt cagcgacatc gtcacgatac ggttatcctg caaataacgc
    12481 ttcagctgtt tcagtagctg gcgcagttgt gcaatgtctt cttcgtaacg gcattgttca
    12541 gcaaattcgg tcagcgtaat ggaaggcttg ccgtcaaaat cggcaatcac atcgtagaca
    12601 taaccggttc cgcaatccgt ctcaacggta ccgtgatagc gcggtattcc actccagtct
    12661 tttaaccggc gaccaagatg cgcgtagtac tttaactcac ggcggatctc tttatcgcca
    12721 ccatcgccac gatggtagac aatcttgata cagcgttggg catcttccgg atgcgcataa
    12781 catttccgat gtcttccggt gcccagggga ctttgttcag ataagcgaat catgccatct
    12841 cctggaaata ttttctgacg atctccagta tggtcgatta acttaaacaa aacctaaaca
    12901 atgcctctta aggttttctt aaggttcttc tgaaagtgaa aagcgcacaa aatgtacaaa
    12961 tgacgttgat tgagaacgat ttcagtacgt gattttctta ttgtcggggg agcttgaagg
    13021 gagttgtatg tcaaagtgat gaagattaat cttcatcaaa accagaatta aagagggcga
    13081 taacggcggc cagtgcttcc tcttcctgtg gaccggtcgc ttcaacttca atctgccgtc
    13141 ctttggcaga atccaacatc agcagcgcaa taacgctgtt ggcttcagcc tcggtgcctt
    13201 cgtcattacg taacagcact tcagcgtcaa aaccctgcat taattcaaac agcttcattg
    13261 caggccgggc atgcatgccc agcttgtttg tgatttcaac agtttgcttg acggtcatgg
    13321 tttacgtttt tccagcgtac gatggcgtga ctggacgttt ttaccgcgcg agcggaagta
    13381 gtctgccagt tgctctgcaa tatacaccga acggtgcttc ccgccggtac aaccaatggc
    13441 gaccgtcagg tagctacggt tgttggtttc cagcataggt aaccatagct caagatagct
    13501 tcgcgtctgg tagataaaat tgtgtacttc tgtgtggcgg tcgaggaacg cggcgacagg
    13561 tttatcaaga cctgtcattg gacgcagttt cggatcccag tgcgggttcg gcaagaagcg
    13621 cacgtcaaag acgtaatctg catcgatagg gataccgtgt ttgaagccga aagactcaaa
    13681 gaccatggtc agttcgcgtt cacgtttacc cagcagacgg gtacgcagca tttcagccag
    13741 ctcgtgaacg gacatttctg aggtatcgac aatcagatcc gctcgcgaac gcagaggctc
    13801 cagcaaatcg ctttctttgt cgatagcact ttccagcgac aggtttttgc tggaaagcgg
    13861 atgcagtcgg cgcgtgtcac tgtaacgacg aattaaggta ttacggtcgg catccaggaa
    13921 cagtagttgc ggtgagaaag cgtcaggcag gttactcatc gcctgttcga atatttctgg
    13981 tgactccggc atattacgaa catcaatgct gacggcggca gaaatctcgc gatcggccag
    14041 agttcgagcc agatcgggta acaacactac gggaaggtta tccacgcagt aaaaacccat
    14101 atcttccagc gcacgcaggg cgacagattt acctgaacct gaacgtccgc tgacgatcat
    14161 cagtaccatg tactgtttct cctcacaacg tctaaaagag acattaccga ataactacgc
    14221 ttcatccgga gtaccttcgg tatccgtaat gatttgatac agctcttcat cgctctgggc
    14281 tgcgcgtaaa cggcggcaga tggttttgtc cgccagacgt ttcgccacca gcgacagagt
    14341 atgcaggtgc gttttagttt ggtctgccgg caccagcagg gcaaaaagta agtccaccgg
    14401 ttggttgtcg atggcatcga aagctatagg cgtttcgagc tgaacgaaaa cgccaacggc
    14461 acgcagagta tcttcttcaa gtttgccatg cggaatggca ataccattgc cgataccggt
    14521 actgcccatt ttttcccgcg tcaggatagc ttcaaaaacc acctgaggcg gcaggctaag
    14581 ttgtttcgcc gccagctcgc tgatgatttc cagggcgcgt tttttgctct gacagtggac
    14641 gcggcttcgc gtacattccc tgttaagaac actgctaagc tgtagagttg tatcattatt
    14701 tgtcataatt tcacctaaga acctgcccac tcaaacagtg gaacaggctc ggagcattaa
    14761 ccgtacaaat ggcccgttgt gccgcacaac aggccgccat gcacatgcta attgcccgga
    14821 caattagtgt tgtttcagtt tatctttatg tttggtcaac tggcgtgcca gcttatcaat
    14881 taagccatca atggcagcgt acatgtcctg accttccgcg ctggcatgaa tttcaccacc
    14941 gtttacatgc agtgttgcat ctgaggtgtg ggtgactttc tccactttca gaacaacata
    15001 gacctggttg attcggtcaa aatattgctc aagtttggca aatttagctg taacaaattc
    15061 gcgcagtgcc tcggtgatct cgacgttatt tccggtaatg ttgagctgca tagtgtcttc
    15121 cttatcggtt gggtcaaacg agctgtttac gctggtttga cggcggaatg gataaagact
    15181 ctcggtactt cgcaacagtg cggcgtgcca ccatgatacc ttgttccgac agcaaagagg
    15241 ttaacttgct gtcgctcaac ggtttcgctg ggttttccgc cgcgattaat ttcttcacca
    15301 gtgcacgaat cgccgtggag gaagcttcgc cgccgccctc ggtattgacg tgactggaaa
    15361 agaaatactt cagttcaaaa atgcctcgtg gactatgcag gtatttttgc gtggtcacgc
    15421 gagatatcgt cgattcatgc atttcgacag cctgggcgat atcggccagt accatcggtt
    15481 tcatatattc ttcaccttgc tcaaagaagg cttgctgctg ttcaacgata cagcgactca
    15541 cgcgcagtag cgtatcgtta cggctttcca gactcttgat caaccatttg gcatcctgca
    15601 gattgctgcg gataaactgg ctgtcaccat cgttgcgcgc gttattgcac atcgaggcgt
    15661 agtgctggtt gatttgcaga cgcggaatgc tgtcactgtt gagttctacc gtccagtgac
    15721 cgttatgctt acgcaccagc acatctggaa tgacatactc aggttcgcca gtctggatcg
    15781 actgcccggg gcgcggatcg agcgactgga tcagattgac ggcttctttc agcacatctt
    15841 ctttcagacg cgtgacgcgc attaaagtgc ggaagtcgtg attggctaac agatcgagat
    15901 gatcgctaat gatcaatctg gcctcttcca gccacggcgt ggtcttatcg aattgggaga
    15961 gttggattag caggcagtca cgcagatctt ttgccgctac accgaccggg tcaaaccgtt
    16021 ggatccgctt aaggacggct tcaacctcgt cgatgtcgat ctcttcatcg cccatacttt
    16081 cgagaatatc ttccagcggg acagtcagat aaccggtttc atcaacggca tcgacgatag
    16141 aggtagcaat agcgcggtca gtgtcggaaa acggtgtcag ctcgacctgc cacatcaggt
    16201 aatcctgcaa ggtctgcgtc gtttcgccct ggtagaccgg cagctcgtcg tcaatgtagt
    16261 caccgctggt gccggatggt gtaccagcgg tgtaaatggt gtcccaactg gcatcgagcg
    16321 gcagctcttc cggcatctct ttttgttcga gcgcgtcggc ggtgtccagc gtttcactgt
    16381 cttgcgtttc gcgggtgtcg atttcttcat gagtgtcgat ttgctcaagc agcggattac
    16441 tctccagcgc ctgctgtagc tcctgctgaa gttccagcgt cgacaactgc aacagacgaa
    16501 ttgcctgttg gagctgtggc gtcatcgcca gttgttggct aagcctgagt tgcaaacctt
    16561 gcttcatgtt cagaatcgta ctctcctgct aaaacgtcgc aaacttctac cctatcagag
    16621 tctgaagtct tccccaaggt atacacgctt aacgtgttcg tcttgtaaga tttctgtagg
    16681 cgtgccgtgg gcgatcaaat gcccctgact gacgatataa gcgcgttcac aaaccgccag
    16741 tgtttcacgc acgttgtggt cagtgatcag cacgcccagg ccgctgtcgc gcaggtgttc
    16801 aatgatgcgt ttaatgtcga taaccgagat cgggtcaacc ccggcaaacg gttcgtcgag
    16861 cagaataaat ttcggattcg cagccagtgc gcgggcaatt tctacacggc gacgttcacc
    16921 cccggagagt gactgcccca tgctgtcacg caggtgctca atgtgaaact cttccatcag
    16981 ctcgttcgcg cggtcttcac gttgttcagc agacaagtcg tcacgaattt gcagtaccgc
    17041 catcaggtta tcgtaaacgc tgaggcgacg gaaaatggag gcttcctgtg gcagatagcc
    17101 gataccgcgg cgcgcgcgtg catgcagagg cagcagactg atatcgtcat catcaataat
    17161 gatgttgccc gcatcgcgcg gcacaatgcc tacaaccatg tagaaagtgg tggtcttacc
    17221 ggcaccgttt ggccccagca gaccgacaat ttccccggag ttgacggtca ggctgacgtc
    17281 ttctaccacg cgacggcctt tataggcttt tgcaaggttc tttgcagtta atgttgccat
    17341 aacgaattaa ttacccttct tctgtgccgg ggtctggcct ttgttgtttt tgtcctgcag
    17401 ctgcgacggc accagaacgg tggttacgcg cttgcctttg tcgctgaaag cctgcatttt
    17461 ctgctctttc accaggtaag tgatcttatc gcccttaatg ttgctatcga cctgctgcag
    17521 ataagcatta cccgtcagaa cgacaaaatc ttttgccagt tcgtagtgca tctgggaagc
    17581 gtgaccttca acgggtttac cgttgtcctg catctggtag aacgttgccg gtttaccgta
    17641 tccgtcaatc acttctttac cttgttcgcc gcccggacgg gtaacgacca ctttgtcggc
    17701 attaattttg atggtgccct gggtgacgat gacattaccg gtaaaggtaa ccacgttgcc
    17761 ttgcatatca agagattgct ggtccgattc aatgtggatc ggctgatcag tgtctccggt
    17821 tacggcaaat gccggaatgc tggcggccag aagtgagctg gcaagcacaa gattaaggct
    17881 gagtttgttt gttttgaatt tcataggatg ttctaacctt ttcaatcagc tcggcgttct
    17941 tgctgcgtaa gttgccgcgc attttcagac cgctggagtt aaatgttgtt ccgtataacg
    18001 tgacgaggtc ttcagaggta acatcctgcg tcaccagatt gatctgcgcg ttatccgtcg
    18061 tgattctgcg aagttgagag tctggcacaa gtgcgttgac ttcaacgtgt ccatataaat
    18121 agagcatccg gtcattggtc agcttggctt tatctgcttt tacggaccat gtcgggattt
    18181 tatccttatc aaacgtggta agtaccggct gcgtaaacca cgaaacggcc tgatcggaat
    18241 aatattcaac gtgttgagca atcaatcgat agcttagtgc cccttctggg ttatagacga
    18301 gcgtgtccgt atgctcgctt ttataggtgg gatcattgtt gttgacgacc acctgggcgg
    18361 tatcgtcttt ttcggccata ttaatgccga tcatcaccag aaccgccagt gatagcacaa
    18421 tgataaccca acgtctggct ttactcatat cgattgccct ttggcttcat ccagtttgcc
    18481 ctgcgccagg agtaataagt cgcaaacttc gcgcactgcg ccacgaccgc cagcaatgcg
    18541 cgtcacgtaa tcggcgcgcg ggatcaacag tggatgcgca tcggccacgg cgacgcttaa
    18601 acccactttt tccattaccg gccagtcgat gagatcatcg ccaacataag ccacattttc
    18661 cggggcaatc gccaattttt ccagcaaatc gctaaaggcg atcagtttgt ttgactgccc
    18721 ctgatacaag tgagtgatcc ccaatgtggc acaacgatct tctaccagtt tagcctttcg
    18781 cccggtaatg atagcgactt caatatcaga ggtgagcgca caacgaatgc cataaccgtc
    18841 acgaacattg aacgctttca gctcttcgcc gttattgccc atataaatca ggccatctga
    18901 cagtacgcca tcaacgtcga ggatcagcag acgaatgttc tctgcttttg ccataacgtc
    18961 ggcgctgaca gggccgtaac aggtcgcaag cgacgcacct gctttgctca ttgttgttta
    19021 tccttgaatc tttacactac gcctgcacgc agtaaatcat gcatatgtaa cacaccgagt
    19081 aaatggtcgc catcggcaac catcacggag gtgatatggc gggactgcat taagttcagt
    19141 gcctcaacgg ccagaatgcc agggcgcaca cgtattcccc ccggcgtcat cacatcggca
    19201 atacttaact gacgaacatc cacgcccata tcgaagacac ggcgtaaatc accgtcggta
    19261 aagatgcctt caatcatcat attgtcatcg caaatgacag tcataccaag atttttgcgg
    19321 gtaacttcca gcaatgcgtc acgcagactg gccgttttct taacatgcgg gatctcatcg
    19381 cccgtatgca taatatcgtt tacgcgcagc agaagtttac gacccagtgc gccgcctggg
    19441 tgtgagagcg caaaatcttc agcagtaaag ccgcgtgctt ttaacagcgc gacagcgagg
    19501 gcatcgccca taaccagcgt ggcggtggtg ctgctggtcg gtgccagccc taacggacag
    19561 gcttctttcg ctactttaac acacagatgc acatctgcgg cgcgcgccat gctgctctcc
    19621 gggcgaccgg tgatgcagat taacggtacg tgaagacgct taagcactgg aattaaggcc
    19681 gtgatttcgc tggattcacc tgagttagag atagcaatca ccacatcctg tggggtgacc
    19741 atgcctaaat caccatgcgc ggcttcacta ggatggacga aaaatgaagg tgtaccggtg
    19801 ctggcaaacg ttgcggccat tttgcgcccg atgtgccccg atttccccat ccccatgacg
    19861 acaactttcc ctttacacca gaacatcttt tcacaggcaa gcgtgaaatt ctgattgatg
    19921 tattgatcaa gctccgccag gcattcacgt tcaatcgcca ggacttcttt acctgcttgc
    19981 tgaaagtcaa aacccggttg taactctacg tgcgacataa tgcgtttcca gttattcaac
    20041 gagtattggc gataaccagt acaacatcgc caaccatacg ataaatccgc cagttaataa
    20101 tacccctaca ccacggcccg gttgcgggga gcgccgccag cacagcaacg caaaaataat
    20161 gctcaccagc aacatcacgc tgtagtcacg actgtacgcc agtggatcaa tctctcctgg
    20221 cgttatcagc gcgggtaaac ccaacacgat gacaatatta aaaatgtttg cgccaatgat
    20281 atttccgaca gcaatgtcgt tttcaccttt gcgaaccccc gctattgcgg ttgccagttc
    20341 cggcagactg gttccgatag caattgccgt cagagccatc gtcaactcgc tgatggcaaa
    20401 gtaattcgcc agcaccgtgg cgttatcaac caccatccgc gtggccactg gcatgatgat
    20461 aagcgcaatg ccgagccata aaaacgcgac gggcaatccg ccgtcacgcg gcagctctgc
    20521 aagctgctct ctggtcaggc tgtcagtccc ctgacgtgca agtttaacaa tgaacagcag
    20581 ccatagcaca gccagaaaga ggagaaagat accatcgctt cgactaagtt gtccgtcata
    20641 gagtacggaa ccggccacca cgctgaccaa caacattaag ggtaattcac ggcgcagaac
    20701 atcggaatgg acggtaaaag gacgaaccag cgcagccaga ccgaggatca gcaatatatt
    20761 gataatgttt gagccgaggg ctgtaccaac ggctaaatcg cgttgttcgt gcagagacgc
    20821 ggcaagcgag acgatgattt ctggtaacga tgtaccaata ctgaccaccg tcatgccgat
    20881 gatcagcggc gggatgccaa aggttcggca aagaatagac gcggcaaaaa ccaggcggtc
    20941 ggcactgtaa acgaccaaaa gtaaaccaac aattaacagt gccgtagcta aaagcatcta
    21001 acgtcctttc ttcaggtata ctcgccggtc cgctgaagat tttcagaaag ccgtaacgga
    21061 ttcttaattt tgactttatg cggctaaaaa gtaaaacaaa tgccagcttt cgctaaccac
    21121 ggcgggtaat attctgtaaa tatgttgggt tcaaggttaa attgagcgcc atgcttagaa
    21181 aatcaacgca agacgaaggg tgaattatgg agcagtctgt ggcgaattta gtcgatatgc
    21241 gcgatgtcag ttttacgcgt ggcaatcgct gcatcttcga taatatttcc ctgaccgtgc
    21301 cgcgagggaa gatcacggcg atcatggggc catcgggcat cggtaaaacg acgctactcc
    21361 gtctgattgg cgggcaaatc gcaccagatc atggtgagat ccttttcgat ggtgagaata
    21421 ttccggcgat gtctcgttcg cgcctgtata cagtgcgtaa acggatgagc atgttatttc
    21481 agtccggggc gttgttcact gatatgaacg tatttgacaa cgtcgcctat ccactgcgcg
    21541 aacataccca acttcccgcg ccattgttgc atagtacggt gatgatgaag ctggaggccg
    21601 tggggctgcg tggagcggct aaactaatgc cttctgaact ttccggtggg atggcgcggc
    21661 gtgcagcgct ggcgcgtgcg attgcgctgg agccggatct catcatgttt gatgaacctt
    21721 ttgttgggca agatcccatc accatgggcg tactggtgaa gctgatttct gagctgaaca
    21781 gcgcgctggg cgtgacttgt gtggtggttt ctcacgatgt gccggaagtg ttaagtattg
    21841 cggatcacgc ctggatcctg gcggacaaaa aaattgtcgc tcatggcagt gcccaggcgt
    21901 tgcagacgaa tcctgatccg cgcgtacgtc agtttctgga cgggatagct gacgggcctg
    21961 ttccgttccg ctatcctgcc ggcgattatc acgctgatct tttaccaggg agttaagcca
    22021 ctcatgctgt taaatgcgct ggcgtcgctc ggacataaag ggattaaaac cctgagaacg
    22081 ttcgggcggg ccgggttaat gttattcaat gcgctggtcg gcaaaccgga atttcgcaaa
    22141 catgcgccgc tgctggtgcg ccagctctat aatgtcggcg tcctgtcgat gctgattatt
    22201 gtagtttctg gcgtgttcat cggaatggtg ttggggctgc aaggttatct ggttctgacc
    22261 acttatagcg cggaaaccag tctgggtatg ctggtggcgt tatcgctact gcgtgaactg
    22321 gggccggtgg ttgccgcgct gttgtttgcc gggcgtgctg gttcggcgtt aaccgcagaa
    22381 atcggcctga tgcgcgctac agagcaactc tccagtatgg agatgatggc ggtggatccg
    22441 ctgcgtcggg ttatttctcc ccgtttctgg gctggggtta tttcattacc actgttgacg
    22501 gttatcttcg tcgccgtggg gatctggggc ggatcgctgg tcggcgtcag ttggaaaggc
    22561 attgatagcg ggttcttctg gtcggcaatg caaaatgccg tcgactggcg tatggatctg
    22621 gtcaactgtc tgattaagag cgtggtgttc gccatcacgg tgacgtggat ttcgttgttt
    22681 aacggctacg acgccatccc gacgtctgcc gggattagcc gggcaaccac tcgcaccgtt
    22741 gtccactcgt ctctggctgt tctggggctg gattttgtgc tgaccgcatt gatgtttggg
    22801 aattgagttc atgcaaacga aaaaaaatga aatttgggtg ggtatctttt tattagcagc
    22861 actgctggcg gcgctgtttg tttgcctgaa ggcggcgaac gtgacgtcca tacgtactga
    22921 atcgacctac acgctttatg cgacgttcga taacattggc ggcctgaaag cgcgctctcc
    22981 ggtcagcatt ggtggtgttg ttgtgggccg ggtggcggat attacgctgg acccgaaaac
    23041 ctatctgccg cgcgtaacgc tggaaattga acaacgttat aaccacattc ctgataccag
    23101 ttcgctgagc attcgtactt ccggcctgct gggagaacaa tatctggcat taaacgtcgg
    23161 ttttgaagac ccggaactgg ggactgctat cctgaaggat ggcgatacaa ttcaggacac
    23221 caagtctgcg atggtgctgg aagatctcat tggtcagttc ctttacggta gtaaaggcga
    23281 tgacaataag aatagtggcg atgcgccagc tgctgcgcca ggtaataatg aaaccactga
    23341 acctgtgggt acaacgaaat aatttcagga gaaccgacgc atgtttaaac gtttaatgat
    23401 ggtcgctttg ctggtgattg cacctctgag tgcggcaacc gcggcagacc agaccaatcc
    23461 ttataagctg atggacgagg cggcgcagaa aacgttcgat cgcctgaaga atgagcaacc
    23521 gcaaattcgg gccaacccgg attatctgcg taccattgtt gatcaggaac tgctgccata
    23581 cgtacaggtg aaatacgccg gtgcgctggt gctgggccag tattacaaga gtgcgacccc
    23641 tgctcaacgt gaagcctact ttgccgcttt ccgtgagtac ctgaagcagg cttacggtca
    23701 ggcgctggcg atgtatcacg gtcaaaccta tcagattgcg ccagaacagc cgctgggcga
    23761 taaaaccatt gtgcctattc gcgttaccat tattgacccg aatggccgtc cgccggtgcg
    23821 tctggacttc cagtggcgta aaaactccca gacgggaaac tggcaagctt acgacatgat
    23881 tgctgaaggc gtcagtatga tcaccaccaa acaaaacgag tggggaacgc tgctgcgtac
    23941 caaaggtatc gacggcctga ctgcgcaact gaaatcgatt tctcaacaga aaatcactct
    24001 ggaagagaaa aaataatgag cgagtcactg agctggatgc agacgggtga cacgctggcg
    24061 ttatccggag agctggatca ggacgttctg ctaccgcttt gggaaatgcg tgaggaggcg
    24121 gtgaagggga ttacctgcat cgatcttagc cgtgtgtccc gcgtggatac ggggggactg
    24181 gcactgctgc tccatcttat cgatctggcg aaaaagcagg gcaacaacgt gacgcttcag
    24241 ggggtaaacg acaaagtgta taccctcgcg aaattgtata atttgcctgc tgatgttctg
    24301 cctcgttaaa ttttttcagg ccggatatgg cgttaaccgc ctgtccggca aacctgcctg
    24361 caagtcgagc cttgtctgat ttatcagcga ggctttttgc ttatttatgc cagcgcaact
    24421 ttgctctaag atgtttcgct ggtttattaa ctgatgattg aagatcccat ggaaaataat
    24481 gaaattcaga gcgtgttgat gaacgctctc tccctccagg aagtccacgt ttccggcgat
    24541 ggcagccact ttcaggttat tgccgtgggt gagttgtttg acggtatgag tcgggttaaa
    24601 aaacagcaga cggtctatgg cccgctgatg gaatatattg cggataaccg cattcatgct
    24661 gtgtcgatca aagcgtatac ccctgcggag tgggcgcgcg atcgcaaact gaacggcttt
    24721 tgagctatgg gcgattcgcc ggtagcggat atgaattgtt aactgagaac aaactaaatg
    24781 gataaatttc gtgttcaggg gccaacgaag ctccagggcg aagtcacaat ttccggcgct
    24841 aaaaatgctg ctctgcctat tctttttgcc gcactactgg cggaagaacc ggtagagatc
    24901 cagaacgtcc cgaaactgaa agacgtcgat acatcaatga aactgctaag ccagctgggt
    24961 gcgaaagtag aacgtaatgg ttctgtgcat attgatgccc gcgacgttaa tgtattctgc
    25021 gcaccttacg atctggttaa aaccatgcgt gcttctatct gggcgctggg gccgctggta
    25081 gcgcgctttg gtcaggggca agtttcacta cctggcggtt gtacgatcgg tgcgcgtccg
    25141 gttgatctac acatttctgg cctcgaacaa ttaggcgcga ccatcaaact ggaagaaggt
    25201 tacgttaaag cttccgtcga tggtcgtctg aaaggcgcac atatcgtgat ggataaagtc
    25261 agcgttggcg caacggtgac catcatgtgt gctgcaaccc tggcggaagg caccacgatt
    25321 attgaaaacg cagcgcgtga accggaaatc gtcgataccg cgaacttcct gattacgctg
    25381 ggtgcgaaaa ttagcggtca gggcaccgat cgtatcgtca tcgaaggtgt ggaacgttta
    25441 ggcggcggtg tctatcgcgt gctgccggat cgtatcgaaa ccggtacttt cctggtggcg
    25501 gcggcgatct ctcgtggcaa aattatctgc cgtaacgcgc agccagatac tctggacgcc
    25561 gtgctggcga aactgcgtga tgctggagcg gacatcgaag tcggcgaaga ctggattagc
    25621 ctggatatgc atggcaaacg tccgaaggct gttaacgtac gtaccgcgcc gcatccggca
    25681 ttcccgaccg atatgcaggc ccagttcacg ctgttgaacc tggtggctga agggaccggg
    25741 ttcatcaccg aaacggtctt tgaaaaccgc tttatgcatg tgccagagct gagccgtatg
    25801 ggcgcgcacg ccgaaatcga aagcaatacc gttatttgtc atggtgttga aaaactttct
    25861 ggtgcacagg ttatggcaac cgatctgcgt gcatcagcaa gcctggtgct ggctggctgt
    25921 attgcggaag ggacgacggt ggttgatcgt atttatcaca tcgatcgtgg ctacgaacgc
    25981 attgaagaca aactgcgcgc tttaggtgca aatattgagc gtgtgaaagg cgagtaatcg
    26041 tctgagagct ggcagccccg ttcgcggggc taccgccaga ccactcattt tttcggttta
    26101 gtgtagctac gcatcaactg cgttctgtcg ataaactcat gggtctgcgg atcatggtag
    26161 cgtgatggcc agataaccca ggggtcagtt cccagggctt tcgcaataat catctctcct
    26221 ttcggccatg ggcgcgataa tgcgtttgcc agcgttgagg aactcaaacc atttctgcga
    26281 gattccgccg ccattgacgt tcctttcttg cgcaaacctg caatgatgtc agcgggatgc
    26341 cagtcaatga aattactttc catctttcct cactccttaa gttagtcgct ttcattcaga
    26401 acacttcttc tgtcgggcat cactatactc ctgatggtct attgcgctaa aaagttcctg
    26461 taaagagtgt gaatattcag aatatataaa gattcatcgc ccttttttca ggtgttatag
    26521 cgctgaatta gtgtttttat ggaatttact ggaatcatcc cgcgcactgc gcgggatgaa
    26581 gggaggggat taacgatcgc gttgaacagc gatgtgcgcg aggccgatga gtgcttctcg
    26641 ccaaggggtg tccgggagca cctgtaacgc tgcgatggct ttgtctgctt cttcctcggc
    26701 gcgctgacgc gtccattcaa gagatccaca agcgttcatt gcttccagaa ccggttccag
    26761 aagatggcga ccgttacctt gttcgatggc ggtacggatc atctgtgcct gttctggtgt
    26821 gccatgatgc atcgcatgca gcagcggcag cgtcggttta ccttcgttca gatcgtcgcc
    26881 gacgttttta cctaactgtt cgccatcggc attgtaatcg agtaaatcgt cgatcaactg
    26941 gaaagcagtg ccgagatagc gcccataatc ctgcaggcct ttctcctcct ccggcgtaca
    27001 gccagccaga atcccggaac actgcgcggc agcctcaaac agacgcgcgg ttttgctata
    27061 gataacgcgc atgtagtttt cttcagtgat gtccggatcg ttaacgttca tcagttgcag
    27121 aacttcacct tctgcgatga cgtttacagc ttctgacatg acttccagca ctttgagtga
    27181 accgaggctg gtcatcatct ggaacgcgcg ggtataaata aaatcgccta ccagcacgct
    27241 ggcggcattg ccaaatgcgg cgttggcggt agctttaccc ctgcgcatat ctgattcatc
    27301 cacaacgtcg tcgtgtagca gagtcgccgt gtggataaac tcgatcaggg cagcaatggt
    27361 gacatgcgca tttccctcat agccaacagc tcgtgcagcc agtacagcaa tcatcggacg
    27421 aatacgttta ccgccgccgc tgacgatgta atagcctaac tgattgatca gttggacgtc
    27481 ggaattaagc tgctcaagga ttgccgcatt aacacccgcc atatcttgcg cggttaactc
    27541 attgattttt tctaaattca tcgcaaaagc cgggcttttc gccccgatta ccttactgtg
    27601 aacaaaggca acttagggtt tatggttagg aatatagggt aattgtactg aaaaaatggc
    27661 acagataaac gttaccgtac aagttgtgtt tttttcttcg tgtattgact gtagcacttg
    27721 tcaaaggcgt gcgttttgcg taatattcgc gccctattgt gaatatttat agcgcactct
    27781 gaatcattga aaaggtgtgc gcggaagcgg agttttatat gtacgcggtt ttccaaagtg
    27841 gtggtaaaca acaccgagta agcgaaggtc agaccgttcg cctggaaaag ctggacatcg
    27901 caactggcga aactgttgag ttcgctgaag tgctgatgat cgcaaacggt gaagaagtca
    27961 aaatcggcgt tcctttcgtt gatggcggcg taatcaaagc tgaagttgtt gctcacggtc
    28021 gtggcgagaa agttaaaatc gttaagtttc gtcgtcgtaa acactatcgt aagcagcagg
    28081 gccatcgtca gtggttcact gatgtgaaaa ttactggcat cagcgcctaa gacctgagga
    28141 gagatttcaa atggcacata aaaaggctgg cggctccaca cgtaacggtc gcgattcaga
    28201 agctaaacgc ttgggcgtta agcgtttcgg tggcgaatcc gttctggcgg gtagcatcat
    28261 cgttcgtcaa cgtggtacca aattccacgc tggcgctaac gtaggttgcg gtcgtgacca
    28321 cactctgttt gctaaagcag acggtaaagt gaaattcgaa gttaaaggcc cgaaaaaccg
    28381 taaatttatc agcatcgaag ctgaataagt ttttcgcgtc ccggtaacgg atgaaagccc
    28441 cgcaacacgt tgcggggctt tttacattcg ataaccggta aaaatgcgca tggtgcgacg
    28501 ttaatccgca aatgcgcttt gcagggaata cggtatgaag cagcaggcag gcattggcat
    28561 tcttttggcg cttaccacag caatttgctg gggggcgttg ccaatcgcaa tgaagcaggt
    28621 gctggaggtg atggaacctc cgacaatcgt gttttaccgt ttcttgatgg cgagtattgg
    28681 ccttggtgcc attcttgcgg tgaagaagag gttgccgcca ttacgcgtgt ttcgtaagcc
    28741 acgctggttg attttgttgg cagtggcgac cgccgggctg tttgggaact tcatcctgtt
    28801 cagctcatcc ttgcaatacc tgagtccgac cgcttcgcag gtgattgggc aactctcgcc
    28861 agttggcatg atggttgcca gcgtatttat cctgaaagag aaaatgcgca gcactcaggt
    28921 tgtaggggca ttgatgctcc tgagcggtct ggtgatgttt tttaacacca gtctggtcga
    28981 gatatttaca aagctcaccg attacacctg gggagttatc tttggggtcg gtgcggcgac
    29041 ggtttgggtg agttatggcg tggcgcaaaa ggttttattg cgtcggctgg cctcaccgca
    29101 gatcctgttt ttactgtaca ctttatgtac aatcgcgctc ttccctctgg caaagcctgg
    29161 agtgatagcg cagcttagcc actggcagct cgcatgttta attttttgcg gactgaatac
    29221 cttggtagga tatggcgcct tggcggaagc gatggctcgc tggcaggcag cgcaggtgag
    29281 cgcgatcatc acgctcaccc cactgtttac gctgtttttt tcagatcttt tatcactggc
    29341 ctggcccgat ttcttcgcca gaccgatgtt aaacctttta ggttatctcg gtgcgtttgt
    29401 cgtggttgcg ggcgcgatgt attccgccat tggtcatcgt atttggggcg gattacgtaa
    29461 gcatacaacg gtggtatcgc aaccccgcgc aggcgaatga tttacggaga ataaaatgaa
    29521 gtttgttgat gaagcatcga ttctggtcgt tgcaggtgat ggcggtaatg gttgcgtgag
    29581 cttccgccgc gaaaagtata ttccgaaagg cggcccggat ggcggcgacg gcggtgatgg
    29641 tggtgacgta tggatggaag ccgacgagaa cctgaacacg cttatcgatt atcgttttga
    29701 aaaatctttc cgtgcagagc gcggtcagaa cggcgcaagc cgcgattgta ccggtaagcg
    29761 cggtaaagac gtgacgatta aagtgccggt aggtacgcgt gtaatcgacc agggtactgg
    29821 tgaaaccatg ggtgatatga ctaaacacgg tcagcgtctg ctggttgcta agggcggctg
    29881 gcacggtctg ggcaataccc gtttcaaatc gtcagttaac cgtacaccac ggcagaaaac
    29941 taacggtacg ccgggcgata agcgtgagct gctgctggag ctgatgctgc tggctgacgt
    30001 cggtatgttg gggatgccaa acgcgggtaa atcgaccttt attcgtgcgg tatcggcggc
    30061 taaaccgaaa gtggcggatt atccgtttac cactctggtg ccaagtctgg gtgtggtacg
    30121 aatggacaac gaaaagagct tcgttgttgc cgatattcca ggactgattg aaggcgctgc
    30181 ggaaggcgca ggtctgggca ttcgcttcct gaagcacctg gaacgttgcc gcgtcctgtt
    30241 gcacctcatc gatatcgatc cgattgacgg caccgatccg gttgaaaacg cgcgtattat
    30301 tatcagcgag ctggaaaaat acagccagga tctggcggcg aaaccgcgtt ggttagtctt
    30361 caacaagatc gatctgctgg ataaggcaga agccgaagag aaagcgaaag cgatcgctga
    30421 agcgctgggc tgggaagata aatattatct gatctctgcg gcgagcggac tgggcgtgaa
    30481 agatctctgc tgggatgtga tgacctttat cattgaaaac ccggtcgtgc aggctgaaga
    30541 agcgaaacag ccagagaaag tcgaattcat gtgggatgat tatcaccgtc agcagcttga
    30601 agagattgct gaagaggatg atgaagactg ggatgacgac tgggacgaag acgacgaaga
    30661 aggcgttgag ttcatttaca agcgttaatc actaatgacc aatgccaaat aagcctgatg
    30721 cgctacactt atcaggccta caagatgatt gcaatatatt gaatttgcac gattttgtag
    30781 gccggataag gcgttcacgc cgcatccggc ataaacaaag cgcactttgt caataatctg
    30841 aaaccccggc catgcgccgg ggtttctttt tgactaattg ttctgataaa tatctttgta
    30901 caaacggctt tcaaaacgca ctaacggaat acggcgatta cgctgatccg cgggttttac
    30961 tgcatagcca gaaagatatt gtacaaatgc cattcgttgc ccgctcgctg tggtaatgaa
    31021 tcccgccagg ttatataccc cctgcaacga accggttttc gctgagactt tgccatccac
    31081 gcccgcctga tgcagacctg cacggtactg caaagagccg tcatagcccg caagcggcag
    31141 catggagata aagttaagtt cattgtcgtg ttgggcaatg tattgcagca cctgcatcat
    31201 ggtggcgggg gcaatcaggt tatgccgcga aagccctgaa ccatcggcaa taatggtgtt
    31261 accaatatcg acaccggctt gctggcgcag gatctgacgc acggcgtccg acccagcccg
    31321 ccatgttcca ggcacattga agcgcgcatg gccgatcatg cggaaaaccg tatcggcgat
    31381 catgttgtcc gactttttca gcataatctt aagcagatcg tgcagcgggg ccgactgttt
    31441 actggcaact accgttccag gttcgttaac ctgagtctgg cgcagcagtg ttccgctcca
    31501 ggtgataccc gcctgtttta actcatcttt cagaattgca ccggcatagc tggctccatc
    31561 ctgcacggca aaagccaacg ggagcggctc agaacgttgt ggcaggcatc ccgtcagcgt
    31621 aaagcggttc aggtcgcttg gcaccacatc cagttcgcag tattgcgctt cggcagaacc
    31681 acgggggagg gtgcgtacct ggctgaacat cgtaacgggg taataagatg ccacgcgtat
    31741 aaaagccata tcaccaggct ttggggcact gtagagcgag acggagaaac agttgcggtc
    31801 aactatggcg gcagcaggcg gagcgctaaa gcattgtgtc atgtcattcc atggccagcc
    31861 gggggcttta tcgtggctgg cgaaaatgga ggtatctatc aacacattgc catcgatttg
    31921 gttgacgcca gattttttca aagtcgcgac catattgcga atatcctgac gttttaacgt
    31981 cggatcggca ccaaatcgcg ccactaagtc acccttaagt acgccgtttt ccacattgcc
    32041 tttggtttca agcgtcgtgg tgaaacgaaa atcggggccg agttgaatca acgccgccag
    32101 cgcagtaatc actttctggg tactggcggg cagcgccatc tgctgactgt ggtaatcaat
    32161 agcgggggcc gacgcaccga ctttttgcac catcagggca aggttggcac ctgcggggag
    32221 ttgagtaacg tactcatcaa cattcgcggc ctgaacactg aacgctatac agctggtcaa
    32281 tccgatgata aatctggaaa atcgcataat ctcgcgctaa caacctggaa tcgagccgtc
    32341 atactacggc gcaacgccct ataaagtaaa cgatgaccct tcgggaactt cagggtaaaa
    32401 tgaccatcaa aatgtgaatt gtagctgacc tgggacttgt acccgggtcg gtattttttt
    32461 gcttctggtc ccggtgagga gttatgccgg gcaggccgaa cagccggggt gggtgaagac
    32521 ttgccccatc aggaatattc aagaggtata acaaatgcaa gctattccga tgaccttacg
    32581 cggcgctgaa aaattacgcg aagagctgga ttttctgaaa tctgtgcgcc gtcctgaaat
    32641 cattgctgct atcgcggaag cgcgtgagca tggcgacctg aaagaaaacg ccgaatacca
    32701 cgcagctcgt gaacagcagg gcttctgcga aggccgtatc aaagacatcg aagccaagct
    32761 gtcaaacgcg caggtgattg atgtcaccaa aatgcccaac aatgggcgcg ttatttttgg
    32821 tgctaccgta acggtgctga atctggattc tgacgaagaa cagacttatc gcatcgttgg
    32881 cgatgacgaa gctgacttta aacaaaacct gatttctgta aactcgccta ttgctcgtgg
    32941 cctgatcggc aaagaagaag atgatgttgt ggtcatcaaa acgccgggcg gcgaagtaga
    33001 atttgaagta attaaggtgg aatacctgta agaattaccc aatactcaag atgttgatgt
    33061 attgtaaaga aaggaaaaag gccgctctgc ggccttttat caacgaacag agcgtggcat
    33121 tttgctctcc tgcctgcgga aaacccctcg ttttacacag caaatgtgtg taactttagg
    33181 ataatcttag cgtggcagcg agattttacg ttctttagtt gggcgataaa gcaccagcgt
    33241 tttaccgatg acctgtacat tacaggcgcc ggtttcgcgc acgatagctt ccacgatcaa
    33301 ggtttttgtt tcgcgatctt cggtggcgat tttcaccttg atgagttcat ggtgctctaa
    33361 cgcttgttca atctcggcca gcaccccttc ggtcaaacca ttactgccaa gcagaacaac
    33421 tggcttgagc ggatgtgcca gacctttcag gtgctgtttt tgtttagtac tcagattcat
    33481 cgtatttttt gcttacgttg ggattgaaaa cgggtcattc taccgccatc tcccatatat
    33541 caccaaatag gcgcgtaaaa atttacgcaa ttggttacga tgagttatcc ccatgggaaa
    33601 gttaaatgac aggtaagaag cgttctgcca gctccagccg ctggcttcag gaacacttta
    33661 gcgataaata tgttcaacag gcacagaaaa agggattacg ttcccgtgcc tggcttaaac
    33721 ttgatgaaat acagcaaagt gacaaactct ttaaaccggg aatgacggtt gtcgaccttg
    33781 gtgctgctcc gggtggttgg tcacaatatg tggtcaccca aattggcggc aaaggccgca
    33841 tcatcgcttg cgatctttta cctatggatc ctatcgttgg tgtggacttt cttcagggcg
    33901 attttcgtga tgaactggtg atgaaagcac tgctggagcg cgttggcgac agcaaagtcc
    33961 aggttgtcat gtccgatatg gcaccaaaca tgagcggaac accggcggtg gatatccccc
    34021 gagccatgta tctggtggaa ctggcgctag aaatgtgtcg tgatgtatta gcgccaggtg
    34081 gcagttttgt agtgaaggtg ttccagggcg aaggtttcga tgagtatcta agggaaattc
    34141 gctccctgtt tacgaaggtc aaagttcgta agccggactc ttctcgtgca cgttcgcgag
    34201 aagtgtatat tgtagcgacc gggcgtaaac cctaaccggg agatttcaga cgaaagtttg
    34261 aaagatgctg gatatagagt atcctgacgc tgtttttaac acagttgtaa taagaggtta
    34321 atcccttgag tgacatggcg aaaaacctaa tactctggct ggtcattgcc gttgtgctga
    34381 tgtcagtatt ccagagcttt gggcccagcg agtctaatgg ccgtaaggtg gattactcta
    34441 ccttcctaca agaggtcaat aacgaccagg ttcgtgaagc gcgtatcaac ggacgtgaaa
    34501 tcaacgttac caagaaagat agtaaccgtt ataccactta cattccggtt caggatccga
    34561 aattactgga taacctgttg accaagaacg tcaaggttgt cggtgaaccg cctgaagaac
    34621 caagcctgct ggcttctatc ttcatctcct ggttcccgat gctgttgctg attggtgtct
    34681 ggatcttctt catgcgtcaa atgcagggcg gcggtggcaa aggtgccatg tcgtttggta
    34741 agagcaaagc gcgcatgctg acggaagatc agatcaaaac gacctttgct gacgttgcgg
    34801 gctgcgacga agcaaaagaa gaagttgctg aactggtaga gtatctgcgc gagccgagcc
    34861 gcttccagaa actcggcggt aagatcccga aaggcgtcct gatggtcggt cctccgggta
    34921 ccggtaaaac gttgctggcg aaagcgattg caggcgaagc gaaagttccg ttctttacta
    34981 tctccggttc tgacttcgta gaaatgttcg tcggtgtggg tgcatcccgt gttcgtgaca
    35041 tgttcgaaca ggcgaagaaa gcggcaccgt gcatcatctt tatcgatgaa atcgacgccg
    35101 taggccgcca gcgtggcgct ggtctgggcg gtggtcacga tgaacgtgaa cagactctga
    35161 accagatgct ggttgagatg gatggcttcg aaggtaacga aggtatcatc gttatcgccg
    35221 cgactaaccg tccggacgtt ctcgacccgg ccctgctgcg tcctggccgt ttcgaccgcc
    35281 aggttgtggt cggcttgcca gatgttcgcg gtcgtgagca gatcctgaaa gttcacatgc
    35341 gtcgcgtacc attggcaccc gatatcgacg cggcaatcat tgctcgtggt actcctggtt
    35401 tctccggtgc tgacctggcg aacctggtga acgaagcggc actgttcgct gctcgtggca
    35461 acaaacgcgt tgtgtcgatg gttgagttcg agaaagcgaa agacaaaatc atgatgggtg
    35521 cggaacgtcg ctccatggtg atgacggaag cgcagaaaga atcaacggct taccacgaag
    35581 cgggtcatgc gattatcggt cgcctggtgc cggaacacga tccggtgcat aaagtgacga
    35641 ttatcccacg cggtcgtgcg ctgggtgtga ctttcttctt gcctgagggc gacgcaatca
    35701 gcgccagccg tcagaaactg gaaagccaga tttctacgct gtacggtggt cgtctggcag
    35761 aagagatcat ctacgggccg gaacatgttt ctaccggtgc gtccaacgat attaaagttg
    35821 cgaccaacct ggcacgtaac atggtgactc agtggggctt ctctgagaaa ttgggtccac
    35881 tgctgtacgc ggaagaagaa ggtgaagtat tcctcggccg tagcgtagcg aaagcgaaac
    35941 atatgtccga tgaaactgca cgtatcatcg accaggaagt gaaagcactg attgagcgta
    36001 actataatcg tgcgcgtcag cttctgaccg acaatatgga tattctgcat gcgatgaaag
    36061 atgctctcat gaaatatgag actatcgacg caccgcagat tgatgacctg atggcacgtc
    36121 gcgatgtacg tccgccagcg ggctgggaag aaccaggcgc ttctaacaat tctggcgaca
    36181 atggtagtcc aaaggctcct cgtccggttg atgaaccgcg tacgccgaac ccgggtaaca
    36241 ccatgtcaga gcagttaggc gacaagtaag ttcccgcatc agatgactgt atttgtaccg
    36301 aaaaccccgg ggcgtgctcc ggggtttttt cttatcaatt cataccaggg ataacataat
    36361 gaaactcttt gcccagggta cttcactgga ccttagccat cctcacgtaa tggggatcct
    36421 caacgtcacg cctgattcct tttcggatgg tggcacgcat aactcgctga tagatgcggt
    36481 gaaacatgcg aatctgatga tcaatgctgg cgcgacgatc attgacgttg gtggcgagtc
    36541 cacgcgccca ggggcggcgg aagttagcgt tgaagaagag ttgcaacgtg ttattcctgt
    36601 ggttgaggca attgctcaac gcttcgaagt ctggatctcg gtcgatacat ccaaaccaga
    36661 agtcatccgt gagtcagcga aagttggcgc tcacattatt aatgatatcc gctccctttc
    36721 cgaacctggc gctctggagg cggctgcaga aaccggttta ccggtttgtc tgatgcatat
    36781 gcagggaaat ccaaaaacca tgcaggaagc tccgaagtat gacgatgtct ttgcagaagt
    36841 gaatcgctac tttattgagc aaatagcacg ttgcgagcag gcgggtatcg caaaagagaa
    36901 attgttgctc gaccccggat tcggtttcgg taaaaatctc tcccataact attcattact
    36961 ggcgcgcctg gctgaatttc accatttcaa cctgccgctg ttggtgggta tgtcacgaaa
    37021 atcgatgatt gggcagctgc tgaacgtggg gccgtccgag cgcctgagcg gtagtctggc
    37081 ctgtgcggtc attgccgcaa tgcaaggcgc gcacatcatt cgtgttcatg acgtcaaaga
    37141 aaccgtagaa gcgatgcggg tggtggaagc cactctgtct gcaaaggaaa acaaacgcta
    37201 tgagtaatcg taaatatttc ggtaccgatg ggattcgtgg tcgtgtaggg gatgcgccga
    37261 tcacacctga ttttgtgctt aagctgggtt gggccgcggg taaagtgctg gcgcgccacg
    37321 gctcccgtaa gattattatt ggtaaagaca cgcgtatttc tggctatatg ctggagtcag
    37381 cactggaagc gggtctggcg gcagcgggcc tttccgcact cttcactggc ccgatgccaa
    37441 caccggccgt ggcttatctg acgcgtacct tccgcgcaga ggccggaatt gtgatatctg
    37501 catcgcataa cccgttctac gataatggga ttaaattctt ctctatcgac ggcactaaac
    37561 tgccggatgc ggtagaagag gccatcgaag cggaaatgga aaaggagatc agctgcgttg
    37621 attcggctga actgggtaaa gccagccgta tcgttgatgc cgcgggtcgc tatatcgagt
    37681 tttgcaaagc cacgttcccg aacgaactta gcctcagtga actgaagatt gtggtggatt
    37741 gtgcaaacgg tgcgacttat cacatcgcgc cgaacgtgct gcgcgaactg ggggcgaacg
    37801 ttatcgctat cggttgtgag ccaaacggtg taaacatcaa tgccgaagtg ggggctaccg
    37861 acgttcgcgc gctccaggct cgtgtgctgg ctgaaaaagc ggatctcggt attgccttcg
    37921 acggcgacgg cgatcgcgtg attatggttg accatgaagg caataaagtc gatggcgatc
    37981 agatcatgta tatcatcgcg cgtgaaggtc ttcgtcaggg ccagctgcgt ggtggtgctg
    38041 tgggtacatt gatgagcaac atggggcttg aactggcgct gaaacagtta ggaattccat
    38101 ttgcgcgcgc gaaagtgggt gaccgctacg tactggaaaa aatgcaggag aaaggctggc
    38161 gtatcggtgc agagaattcc ggtcatgtga tcctgctgga taaaaccact accggtgacg
    38221 gcatcgttgc tggcttgcag gtgctggcgg cgatggcacg taaccatatg agcctgcacg
    38281 acctttgcag cggcatgaaa atgttcccgc agattctggt taacgtacgt tacaccgcag
    38341 gtagcggcga tccgcttgag catgagtcag ttaaagccgt gaccgcagag gttgaagctg
    38401 cgctgggcaa ccgtggacgc gtgttgctgc gtaaatccgg caccgaaccg ttaattcgcg
    38461 tgatggtgga aggcgaagac gaagcgcagg tgactgaatt tgcacaccgc atcgccgatg
    38521 cagtaaaagc cgtttaaagc gttagataac tggctaaaaa ggcggcgatt gtcgcctttt
    38581 ttctcggcga acacatcggt tttgctgttt ttttccgcag ttgatacaat gcgataaaat
    38641 tgcccttgcg aaggtcattc gttttggtta gtattcacac ccgcttcagt gggaaagatt
    38701 aaaaactccc gctttattgg ttgaagcatt ggtacgcggc aactccgcaa ggaacaggtt
    38761 gattatgtac gaagctcttt tagtagtttt ccttattgtg gcaattggcc ttgttggtct
    38821 gatcatgctg cagcaaggta aaggcgctga tatgggagcc tccttcggag caggcgcttc
    38881 cgctacgctg tttggttcaa gtggttctgg taacttcatg acccgcatga cggcgctgct
    38941 ggcaacgtta ttcttcatca tcagtctggt gctgggtaac atcaatagca acaaaaccaa
    39001 tagaggtagc gaatgggaaa atctgagtgc accggcgaaa accgaacaaa ctcagccagc
    39061 tgctccggct aagccgacca gcgatatccc gaactaaaaa gtagtatccg tgccgaggtg
    39121 gtggaattgg tagacacgct accttgaggt ggtagtgccc aatagggctt acgggttcaa
    39181 gtcccgtcct cggtaccaaa ttccagaaaa gagacgctga aaagcgtctc ttttcgtttt
    39241 ggtcctggtc tgatgaatgg ttgtcaggta tctgtatctc gtccagttgc tttactcgta
    39301 gctggtacct tgaaacgatg gtgccggtac gccttagtta taaacttttc cctactgctg
    39361 acataattgg cactttattg tgtcaactcc agttccggta cgaatacgga tatgtttact
    39421 tctcgataaa ccatcctgct cctgtcagta taactactga tggctacgtt ctgcgtaatg
    39481 tttagagacg cattgataat gttatttaat tactgagaac gcaatggtcg catcatttaa
    39541 aaagaaaaaa ctaacagtgt ttcggtctga tatggatcag agttgaaaaa catgtctgat
    39601 ataaaattga acattgttga taattgttta tattggttgt gaacttggat ttctggatga
    39661 cagtattcaa caaatttgct agaagatttt aaatctcatt ggttgttgta tctttgtgtt
    39721 attgtttttg gtattacgaa cttagtcgcc tcttccggag cgcatatggt tcagcgattg
    39781 ctgttcttcg tcctgaccat cctggttgtt aaacgtatat catcccttcc gcttcgcctg
    39841 cttgttgccg caccatttgt gttactgact gcggcagaca tgagtattag cctctattca
    39901 tggtgtacct ttggtacaac tttcaatgat ggatttgcga ttagtgtgct gcagagtgat
    39961 ccggatgaag ttgtgaaaat gctggggatg tatatccctt atctatgtgc ttttgctttt
    40021 ttatcccttc tttttttggc agtaataata aaatatgatg tttccttgcc gacaaaaaaa
    40081 gtgacaggaa tattattgct gattgtcatt tcgggcagtt tattttccgc ttgtcaattt
    40141 gcttataaag atgcaaaaaa taaaaaagcg ttcagtccat atatactagc gtcgtgattt
    40201 gctacctata cgccgttttt caatctcaac tattttgctt tagcagcgaa agcgcatcaa
    40261 agattactct caattgcaaa cacggtgccg tattttcaat tatcagtcag ggatacaagt
    40321 attgatacct acgtgttgat tgtgggggag tctgtacgtg tcgacaatat gtctttgtat
    40381 ggatatacac gctctacgac accgcaagtt gaagcacaaa gaaaacagat caaactgttt
    40441 aatcaagcaa taagcggcgc accttacact gcgctgtcgg ttcccctttc tttaactgct
    40501 gattctgttt tgagtcatga cattcataat taccccgaca acattattaa tatggctaat
    40561 caagcaggat ttcagacttt ctggctaagc tcgcaatccg cttttcggca gaatggtaca
    40621 gcagttacca gtatcgccat gcgcgccatg gaaacagttt atgtcagagg atttgatgaa
    40681 ttgttgttgc cgcatttatc gcaagcatta cagcaaaata cgcagcaaaa gaaactgatt
    40741 gttcttcatt taaatggaag ccatgaaccg gcttgtagcg cctatccgca atccagcgcc
    40801 gtgtttcaac cgcaggacga tcaggatgcc tgctatgaca actccattca ttacacagat
    40861 agtttgctag gtcaggtttt tgaattatta aaagatcgcc gcgcctcggt catgtatttt
    40921 gccgaccacg gcctggaacg tgaccctacg aagaagaacg tctattttca tggaggcagg
    40981 gaggctagcc agcaggcata tcatgtcccg atgtttatct ggtatagccc cgttcttggg
    41041 gatggcgtgg atcgcacaac ggaaaacaat atcttttcga cagcttacaa taattacctt
    41101 attaatgcgt ggatgggggt aacaaagccg gaacagccgc aaacgcttga ggaagtgatt
    41161 gcacactata aaggagactc acgggttgtg gatgcaaacc atgatgtttt cgattatgtg
    41221 atgctcagaa aggagtttac agaggataag caaggtaacc ccacccctga agggcagggt
    41281 tgatgtcgaa ttactggcct ttgttttcca tattctccac ctgcggcagg cctgaagtgg
    41341 cagaggagga aagcaggcca gttttggcat aaccaaaaag tttctctcgg gtatcagtga
    41401 tatccagatt acgcatggtc aattgaccaa tacgatcatc tggcgagaac accgagtcgc
    41461 ctttttccat cgtcagacgc tctggcttgt aggtcaggtt ctctgagacg gtattcagga
    41521 ttgaataatc gttcccacgg cgcagctcca gggtaacttc accagtgatc tggctggcaa
    41581 cccagcgttg cagagagtca cgcagcatca gcgcctggga gtcaaaccaa cgcccctggt
    41641 acagcagacg gcccaactga cgaccatgcg cgtgatactg ctcaatggtg tcttcgttgt
    41701 gaataccggt caacagacgt tcatacgcaa tgtgcagcag tgccatcccc ggagcttcgt
    41761 aaataccacg gcttttcgct tcgatgatac ggttctcaat ctggtcgctc atgcccaggc
    41821 cgtgacgacc gccgatgcgg ttagcttcca gcatcatttc tacgtcgtcg ctaaaggttt
    41881 taccgttcag cgccaccgga tgaccttgct caaagcgtac tgtgacttct tctgccggga
    41941 tcttcacgct ctcatcccag aatttcacgc ccataatcgg gttgacgatt ttgacgctgg
    42001 agttgaggta ttccagatcc ttcgcttcat gcgttgcacc aagcatgttg gagtctgtgg
    42061 agtaggcttt ttcgacagac attttgtagt cgaaaccgca ggcaatcata aattcagaca
    42121 tctcatgacg gccgcccagt tcatcaataa agtcagtatc aagccacggt ttgtaaatct
    42181 gcagttcagc attggtcagc agaccataac gatagaaacg ttcgatatcg tttcctttat
    42241 aggtgctacc gtcaccccag atattcacgc catcttcttt catcgcagca accagcatgg
    42301 taccagtcac ggcgcggccc agcggcgtcg tgttgaaata ggtcaggccg ccggtggtgt
    42361 tatgaaatgc gccacactga atagcggcaa taccttcggc caccagttgt ttgcggcagt
    42421 cgatcagacg tgcgttctcc gcgccgtatt ccatggcacg acgagggatc gcatcataat
    42481 cctcttcgtc tggctggccc aggtttgcag tatatgcata aggaaccgct cccttttgtc
    42541 gcatccacag cagtgcggca ctggtgtcca gaccgccgga aaaagcgata ccaatacgtt
    42601 gacctaccgg gagatgcttg agaatcgtcg tcataaaata acaccctgct taattaactg
    42661 atgatgagcc tggatttccg ctctcactga atttttatgc aaaataaatg agttttcatt
    42721 taatcatctt ttatcggaga caggaagagt ttagtgtgtt ttttgtaaaa taatgcgctt
    42781 aagggagagc aggggaaggc aaaagtattc aacaaatgaa agtgaactgg atattcattc
    42841 acgtgattag caataaacgt tgacaaaatg tggcatggat cactataatg cctgcagatt
    42901 ttacgtcccg tctcggtaca ccaaatccca gcagtatttg cattttttac ccaaaacgag
    42961 tagaatttgc cacgtttcag gcgcggggtg gagcagcctg gtagctcgtc gggctcataa
    43021 cccgaaggtc gtcggttcaa atccggcccc cgcaaccact ttcccttaga gtcctttttc
    43081 aaatatactg tgaagacttc ggccttcgta gtgggatttg aaaaaatcct tctggaaagt
    43141 gctccagacc gcagttgcgg ttatagggtt cagttatata aagccccgat ttatcggggt
    43201 tttttgttat ctgactacag aataactggg ctttaggccc tttttttatg tcttgggggt
    43261 gggcttgtcc acattagagc aaaaattaac agagatgatt actgcgccag ttgaggccct
    43321 gggttttgaa ctggtaggca tcgaatttat tcgcggtcgc acatccacac tgcgcatcta
    43381 tattgatagt gaagatggca tcaatgttga tgattgtgct gatgtgagcc accaggtaag
    43441 tgctgtgctg gatgttgaag atcccatcac cgttgcttat aacctggaag tctcctcacc
    43501 gggtctcgat cgcccactgt tcacggctga acactacgcc cgttttgtcg gagaagaggt
    43561 gactctggtt ctccgtatgg cggtacaaaa ccgtcgtaaa tggcagggcg ttatcaaagc
    43621 ggtagacggt gaaatgatca cagttaccgt cgaaggtaaa gatgaagtgt tcgcgctgag
    43681 taatatccag aaggcgaacc tggttcccca cttttaatag tctggatgag gtgaaaagcc
    43741 cgcgatgaac aaagaaattt tggctgtagt tgaagccgta tccaatgaaa aggcgctacc
    43801 tcgcgagaag attttcgaag cattggaaag cgcgctggcg acagcaacaa agaaaaaata
    43861 tgagcaagag atcgacgtcc gtgtacagat cgatcgcaaa agcggtgatt ttgacacctt
    43921 ccgtcgctgg ttagttgttg atgaagtcac ccagccgacc aaggaaatca cccttgaagc
    43981 cgcacgttat gaagatgaaa gcctgaactt gggcgattac gttgaagatc agattgagtc
    44041 tgttaccttt gaccgtatca ccacccagac ggcaaaacag gttatcgtgc agaaagtgcg
    44101 tgaagccgaa cgtgcgatgg tggttgatca gttccgtgaa cacgaaggtg aaatcatcac
    44161 cggcgtggtg aaaaaagtaa accgcgacaa catctctctg gatctgggca acaacgctga
    44221 agccgtgatc ctgcgcgaag atatgctgcc gcgtgaaaac ttccgccctg gcgaccgcgt
    44281 tcgtggcgtg ctttattccg ttcgcccgga agcgcgtggc gcgcaactgt tcgtcactcg
    44341 ttccaagccg gaaatgctga tcgaactgtt ccgtattgaa gtgccggaaa tcggcgaaga
    44401 agtgattgaa attaaagcag cggctcgcga cccgggttct cgtgcgaaaa tcgcggtgaa
    44461 aaccaacgat aaacgtatcg atccggtagg tgcttgcgta ggtatgcgtg gcgcgcgtgt
    44521 tcaggcggtt tctactgaac tgggcggcga gcgtatcgat atcgtcctgt gggatgataa
    44581 cccggcgcag tttgtgatta acgcaatggc accggcagac gttgcttcta tcgtggtgga
    44641 tgaagataaa cacaccatgg atatcgccgt tgaagctggt aacctggcgc aggcgattgg
    44701 ccgtaacggt cagaacgtgc gtctggcttc gcagctgagc ggttgggaac tcaacgtaat
    44761 gaccgttgac gacctgcagg ctaagcatca ggcggaagcg cacgcagcga tcgacacctt
    44821 caccaaatat ctcgacatcg acgaagactt cgcgactgtt ctggtagaag aaggtttctc
    44881 gacgctggaa gaactggcct atgtgccgat gaaagagctg ttggaaatcg aaggccttga
    44941 tgagccgacc gttgaagcac tgcgcgagcg tgctaaaaat gcactggcca ccattgcaca
    45001 ggcccaggaa gaaagcctcg gtgataacaa accggctgac gatctgctga accttgaagg
    45061 ggtagatcgt gatttggcat tcaaactggc cgcccgtggc gtttgtacgc tggaagatct
    45121 cgccgaacag ggcattgatg atctggctga tatcgaaggg ttgaccgacg aaaaagccgg
    45181 agcactgatt atggctgccc gtaatatttg ctggttcggt gacgaagcgt aataaactgt
    45241 agcaggaagg aacagcatga cagatgtaac gattaaaacg ctggccgcag agcgacagac
    45301 ctccgtggaa cgcctggtac agcaatttgc tgatgcaggt atccggaagt ctgctgacga
    45361 ctctgtgtct gcacaagaga aacagacttt gattgaccac ctgaatcaga aaaattcagg
    45421 cccggacaaa ttgacgctgc aacgtaaaac acgcagcacc cttaacattc ctggtaccgg
    45481 tggaaaaagc aaatcggtac aaatcgaagt ccgcaagaaa cgcacctttg tgaaacgcga
    45541 tccgcaagag gctgaacgcc ttgcagcgga agagcaagcc cgtcgtgagg cagaagaatc
    45601 ggctaaacgc gaggcgcaac aaaaagctga acgtgaggcc gcagaacaag ctaagcgtga
    45661 agctgctgaa caagcgaaac gtgaagctgc ggaaaaagac aaagtgagca atcaacaaga
    45721 cgatatgact aaaaacgccc aggctgaaaa agcccgccgt gagcaggaag ctgcagagct
    45781 caagcgtaaa gctgaagaag aagcgcgtcg taaactcgaa gaagaagcac gtcgcgttgc
    45841 tgaagaagca cgtcgtatgg cggaagaaaa caaatggact gataacgcgg aaccgactga
    45901 agattccagc gattatcacg tcactacttc tcaacatgct cgccaggcag aagacgaaag
    45961 cgatcgtgaa gtcgaaggcg gccgtggccg tggtcgtaac gcgaaagcag cgcgtccgaa
    46021 gaaaggcaac aaacacgctg aatcaaaagc tgatcgtgaa gaagcacgcg cagcagtacg
    46081 tggcggtaaa ggcggaaaac gtaaaggttc ttcgctgcag caaggcttcc agaagcctgc
    46141 tcaggccgtt aaccgtgacg ttgtgatcgg cgaaactatc accgttggcg aactggcgaa
    46201 caagatggcg gttaaaggct ctcaggtcat caaagcgatg atgaaactgg gcgcaatggc
    46261 aaccatcaac caggttatcg atcaggaaac cgcacagctg gttgctgaag agatgggcca
    46321 taaagttatc ctgcgtcgtg aaaacgagct ggaagaggcg gtaatgagcg accgtgacac
    46381 gggtgctgcg gctgaaccgc gcgcgccggt tgtgaccatc atgggtcacg ttgaccacgg
    46441 taaaacctct ctgctggact acattcgttc aacgaaagtg gcctctggcg aagcgggcgg
    46501 cattacccag cacattggtg cataccacgt tgaaactgaa aacggcatga tcaccttcct
    46561 ggacaccccg gggcacgccg cgtttacttc aatgcgtgct cgtggtgcgc aggcaacgga
    46621 catcgtagtc ctggttgttg ctgccgacga cggcgtaatg ccgcagacga tcgaagcaat
    46681 ccagcacgcg aaagcggcgc aggttccggt ggtcgttgca gtgaacaaga tcgataaacc
    46741 agaagccgat ccggatcgcg ttaagaacga actctcccag tacggcatcc tgccggaaga
    46801 gtggggcggt gaaagccagt tcgtacatgt gtctgcgaaa gcgggtactg gtatcgatga
    46861 actgctggac gctatcctgc tgcaggcgga agttctggag ctgaaagcgg tacgtaaagg
    46921 tatggcgagc ggtgcggtta tcgaatcctt cctcgataaa ggtcgtggtc cggttgctac
    46981 cgttctggta cgtgaaggta ctctgcacaa gggcgatatc gttctgtgtg gcttcgaata
    47041 cggtcgtgtt cgtgcgatgc gtaacgaact gggtcaggaa gtgctggaag cgggtccgtc
    47101 cattccggtg gaaatcctcg gcctgtccgg cgtaccggct gcgggtgatg aagttaccgt
    47161 tgtacgtgac gagaagaaag cgcgtgaagt tgcactgtat cgtcagggta aattccgcga
    47221 agttaaactg gcgcgtcagc agaaatctaa actcgagaac atgttcgcca acatgaccga
    47281 aggcgaagtt cacgaagtga acatcgtcct gaaggcagac gtacagggtt ctgtggaagc
    47341 gatctccgac tccttgctga aactgtctac tgacgaagtt aaagtgaaga tcatcggttc
    47401 tggcgtaggt ggtatcaccg aaaccgacgc caccctggct gcggcgtcca acgccatcct
    47461 ggttggcttc aacgtacgtg ctgatgcctc tgcacgtaaa gtgattgaag cggaaagcct
    47521 ggatctgcgt tactactccg tcatctataa cctgattgac gaagtgaaag cggcgatgag
    47581 cggtatgctg tctccggaac tgaaacagca gattatcggt ctggcggaag ttcgtgacgt
    47641 gttcaaatcg ccgaaatttg gtgccatcgc aggctgtatg gttaccgaag gtgtggttaa
    47701 acgtcacaac ccgatccgcg ttctgcgtga caacgtggtt atctacgaag gcgagctgga
    47761 gtccctgcgc cgcttcaaag atgacgttaa cgaagtccgt aacggtatgg aatgtggtat
    47821 cggcgttaag aactacaacg acgtccgcac tggcgatgtg atcgaagtat tcgaaatcat
    47881 cgagatccaa cgtaccatcg cttaaggttt ttagggttcc atgcgggata aggcgtttac
    47941 gccgcatccg gcatggattg cctgatgtga cgctggcgcg tcttatcagg ccaacgtgaa
    48001 cagcccttcc acctgaaatt aattttaaaa aggggctaac agcccctttt ttgtcaggag
    48061 aatttattat ggcgaaagaa tttggtcgcc cgcagcgcgt agcgcaggaa atgcaaaaag
    48121 agatcgctct catcctgcag cgtgaaatta aagatcctcg cctgggcatg atgaccaccg
    48181 tttccggtgt cgaaatgtct cgcgacctgg cgtatgccaa agtatatgtg acgttcctca
    48241 acgacaaaga tgaagacgcg gttaaagcgg gcatcaaagc gttgcaagaa gcttctggtt
    48301 tcatccgcag cctgctgggg aaagcgatgc gcctgcgtat cgtgccggaa ctgaccttct
    48361 tctacgacaa ctctctggtt gaagggatgc gcatgtcaaa cctggtgacc agcgtggtca
    48421 aacatgacga agaacgtcgt gttaacccgg acgacagcaa ggaggactaa tgagtcgtcc
    48481 tcgtcgtcgc ggtcgcgaca ttaacggcgt tttgttgctg gataaaccgc agggtatgtc
    48541 cagcaatgat gcgctgcaaa aagtgaaacg gatttataac gccaaccgtg ccgggcatac
    48601 cggtgcgctg gacccgctgg caactggcat gttgccaatt tgcctcgggg aagcgacgaa
    48661 gttttcccag tatctgctgg actccgacaa acgctatcgg gtcattgcgc gtcttggaca
    48721 gcgtaccgat acttctgatg ccgacggaca aatcgttgaa gaacgtccgg taacctttag
    48781 cgcagagcaa ctggcggcgg cgctggatac tttccgtggc gatatcgaac agatcccttc
    48841 gatgtattca gcactgaaat atcagggtaa aaaactgtac gaatatgcgc gtcagggcat
    48901 tgaagtaccg cgtgaagcgc gcccgattac cgtttatgaa ttgctgttta ttcgccacga
    48961 aggcaatgag ctggagctgg aaattcactg ctcaaaaggc acttatatcc gcaccatcat
    49021 tgatgacctg ggtgaaaaac tcggttgtgg cgcgcatgtt atttacctgc gtcgtctggc
    49081 ggtaagtaaa tatccggttg aacggattgt gaccctggaa catctgcgtg aactggtcga
    49141 acaagccgaa cagcaggata ttccggctgc ggagttactt gatccattac tgatgccaat
    49201 ggacagtcca gcttcggact acccggtggt gaatcttccg ttaacgtctt ctgtttactt
    49261 caaaaatggt aacccagttc gtacatccgg tgcgccgctg gaaggactgg ttcgcgttac
    49321 agaaggtgag aacggcaaat ttatcggtat gggcgaaatt gacgatgaag gccgcgttgc
    49381 gcctcgtcgc ctggtggttg aatacccggc gtaatgttaa ccgtcttgcg ataacaggtc
    49441 gctacgagta gaatactgcc gcttaacgtc gcgtaaattg tttaacactt tgcgtaacgt
    49501 acactgggat cgctgaatta gagatcggcg tcctttcatt ctatatactt tggagtttta
    49561 aaatgtctct aagtactgaa gcaacagcta aaatcgtttc tgagtttggt cgtgacgcaa
    49621 acgacaccgg ttctactgaa gttcaggtag cactgctgac tgcacagatc aaccacctgc
    49681 agggccactt tgcagagcac aaaaaagatc accacagccg tcgtggtctg ctgcgcatgg
    49741 tttctcagcg tcgtaaactg ctcgactacc tgaaacgtaa agacgtagca cgttacaccc
    49801 agctcatcga gcgcctgggt ctgcgtcgct aattcttgcg agtttcagaa aagggggcct
    49861 gagtggcccc ttttttcaag ctgacggcag caattcactg gaaactaatg tattgttgct
    49921 atgaatgatc ttccgttgca gaggttcgcg cggctaatga gaggctttac ccacatagag
    49981 ctgggttagg gttgtcatta gtcgcgagga tgcgcagaag atcgggtatt aacaccagtg
    50041 ccgtaaggta ctgtctaaga aagagaaagg atattacatt gcttaatccg atcgttcgta
    50101 aattccagta cggccaacac accgtgactc tggaaaccgg catgatggct cgtcaggcta
    50161 ctgccgctgt tatggttagc atggatgaca ccgcggtatt tgttaccgtt gttggccaga
    50221 aaaaagccaa accaggtcag gacttcttcc cactgaccgt taactatcag gagcgtacct
    50281 acgctgctgg tcgtatcccg ggtagcttct tccgtcgtga aggccgccca agcgaaggcg
    50341 aaaccctgat cgcgcgtctg attgaccgcc cgattcggcc gctgttcccg gaaggcttcg
    50401 tcaacgaagt tcaggttatc gccaccgtgg tttctgttaa cccgcaagtt aacccggata
    50461 tcgtcgcgat gattggtgct tccgcagcac tgtctctgtc tggtattccg ttcaatggtc
    50521 cgattggtgc tgctcgcgta ggttacatca atgaccagta cgtactgaac ccgactcagg
    50581 acgagttgaa agagagcaaa ctggatctgg ttgttgccgg tactgaagcc gctgtactga
    50641 tggttgaatc tgaagctgaa ctgctgagcg aagaccagat gctgggcgca gtagtgttcg
    50701 gtcatgaaca acagcaggtt gttattcaga acatcaatga actggtgaaa gaagccggta
    50761 aaccacgttg ggactggcag ccggagccgg taaacgaagc gctgaacgcg cgcgttgctg
    50821 cactggctga agctcgcctg agcgatgctt accgcatcac cgacaaacaa gagcgttatg
    50881 cgcaggttga tgtcatcaaa tctgaaacca tcgcgacgct gcttgctgaa gacgaaaccc
    50941 tggacgaaaa cgaactgggt gaaattctgc acgcgatcga gaaaaacgtt gttcgtagcc
    51001 gcgtactggc aggcgaaccg cgtatcgacg gtcgtgaaaa agatatgatc cgtggtctgg
    51061 atgtgcgtac tggcgtgctg ccgcgtactc acggttctgc gctgttcacc cgtggtgaaa
    51121 cgcaggcgct ggttaccgca acgctgggta ctgctcgtga cgcgcaggtt cttgatgaac
    51181 tgatgggcga acgtaccgat accttcctgt tccactacaa cttccctccg tactccgtag
    51241 gcgaaaccgg catggtcggt tctccgaagc gtcgtgaaat tggtcacggt cgtctggcga
    51301 agcgcggcgt gctggcagtc atgccggata tggacaaatt cccgtacacc gtacgtgttg
    51361 tgtctgaaat cactgaatcc aacggttcct cttctatggc ttccgtgtgc ggcgcgtctc
    51421 tggcgctgat ggacgcaggt gtgccgatca aagctgccgt tgcgggtatc gcaatgggtc
    51481 tggtgaaaga aggcgacaac tacgttgtac tgtctgacat tttgggcgac gaagatcacc
    51541 tgggcgatat ggacttcaaa gttgcgggtt cccgcgaggg tatctctgca ttgcagatgg
    51601 atatcaaaat tgaaggtatc accaaagaga tcatgcaggt tgcgctgaac caggctaaag
    51661 gtgcgcgtct gcatatcctg ggcgtaatgg aacaggcgat caacgcgccg cgtggcgata
    51721 tctctgagtt cgcaccacgt atccatacca tcaagatcaa cccggacaag atcaaagacg
    51781 ttatcggtaa aggcggctct gttatccgtg ccctgaccga agaaactggc accaccatcg
    51841 aaatcgaaga tgacggtact gtgaagattg cagcgaccga cggcgagaaa gcgaaacatg
    51901 ctattcgtcg tatcgaagag atcactgcag aaatcgaagt gggccgcgtc tacactggta
    51961 aagtgacccg tatcgttgac tttggcgcat ttgttgccat cggcggcggt aaagaaggtc
    52021 tggtacacat ctctcaaatc gctgacaaac gcgttgagaa agtgaccgat tacctgcaga
    52081 tgggtcagga agtaccggtg aaagttctgg aagttgatcg ccagggccgt atccgtctga
    52141 gcattaaaga agcgactgag cagtctcaac ctgctgcagc accggaagct ccggctgctg
    52201 aacagggcga gtaaggttgc catttgccct ccgctgcggc ggggggcttt taaccgggca
    52261 ggacgccttg ttagcaaccg ggaacaggac gttcattcaa ccgtggtctt cgggagtggg
    52321 aaatgaagcc ttttttgcgc tggtgtttcg ttgcgacagc acttacgctt gcaggatgca
    52381 gtaatacttc ctggcgtaaa agtgaagtcc tcgcggtacc attgcaaccg actttacagc
    52441 aggaagtgat tctggcacgt atggaacaaa tccttgccag tcgggcttta accgatgacg
    52501 aacgcgcaca gcttttatat gagcgcggag tgttgtatga tagtctcggt ctgagggcat
    52561 tagcgcgtaa cgatttttcg caagcgctgg caatccgacc ggatatgcct gaagtattca
    52621 attacttagg catatattta acgcaggcag gcaattttga tgctgcctat gaagcgtttg
    52681 attctgtact tgagcttgat ccaacttaca actacgcgca cttgaatcgc gggattgcat
    52741 tatattacgg cggtcgtgac aagttagcgc aagatgatct gctggcgttt tatcaagacg
    52801 atcccaatga tcctttccgt agtctgtggc tttatctcgc cgagcagaag ctcgatgaga
    52861 agcaggctaa agaagtattg aaacagcact tcgaaaaatc ggataaggaa cagtggggat
    52921 ggaacattgt cgagttctac ctgggcaaca ttagcgaaca aacgttaatg gaaaggctca
    52981 aggcggacgc aacggataac acctcgctcg ctgagcatct cagtgaaacc aacttctatt
    53041 taggtaagta ctacctaagt ctgggggatt tggacagcgc cacggcactg ttcaaactgg
    53101 cggttgccaa caacgttcat aactttgttg agcaccgata cgcattgttg gaattatcgc
    53161 tcctgggcca ggaccaagat gacctggcag aatcggacca gcaatagctg acgtacacat
    53221 cagcccgtaa tcttttttga ttgccatcac cttaacgggt gagggcgttg ttgttcgtta
    53281 atacacctac tttgagccgg ttcacacttt tcaatgaaaa ttgctgatca atttcatgat
    53341 gagttatgta gactggccgc cattaatttt gaggcacacg tactacatgg ctgaattcga
    53401 aaccactttt gcagatctgg gcctgaaggc tcctatcctt gaagccctta acgatctggg
    53461 ttacgaaaaa ccatctccaa ttcaggcaga gtgtattcca catctgctga atggccgcga
    53521 cgttctgggt atggcccaga cggggagcgg aaaaactgca gcattctctt tacctctgct
    53581 gcagaatctt gatcctgagc tgaaagcacc acagattctg gtgctggcac cgacccgcga
    53641 actggcggta caggttgctg aagcaatgac ggatttctct aaacacatgc gcggcgtaaa
    53701 tgtggttgcc ctgtacggcg gccagcgtta tgacgtgcaa ttacgcgccc tgcgtcaggg
    53761 gccgcagatc gttgtcggta ctccgggccg tctgctggac cacctgaaac gtggcactct
    53821 ggacctctct aaactgagcg gtctggttct ggatgaagct gacgaaatgc tgcgcatggg
    53881 cttcatcgaa gacgttgaaa ccattatggc gcagatcccg gaaggtcatc agaccgctct
    53941 gttctctgca accatgccgg aagcgattcg tcgcattacc cgccgcttta tgaaagagcc
    54001 gcaggaagtg cgtattcagt ccagcgtgac tacccgtcct gacatcagcc agagctactg
    54061 gactgtgtgg ggtatgcgta aaaacgaagc actggtacgt ttcctggaag cggaagattt
    54121 tgatgcggcg attatcttcg ttcgtaccaa aaacgcgact ctggaagtgg ctgaagctct
    54181 tgagcgtaac ggctacaata gcgccgcgct gaacggtgac atgaaccagg cgctgcgtga
    54241 gcagacactg gaacgcctta aagacggtcg tctggacatc ctgattgcga ccgacgttgc
    54301 ggcccgtggc ctggacgttg agcgtatcag cctggtggtt aactacgata tcccgatgga
    54361 ttctgagtct tacgttcacc gtatcggtcg taccggtcgt gcgggtcgtg ctggccgcgc
    54421 gctgctgttc gttgagaacc gcgagcgtcg tctgctgcgc aacattgaac gtactatgaa
    54481 gctgactatt ccggaagtag aactgccgaa cgcagaactg ctgggcaaac gccgtctgga
    54541 aaaattcgcc gctaaagtac agcagcagct ggaaagcagc gatctggatc aataccgcgc
    54601 actgctgagc aaaattcagc cgactgcaga aggtgaagag ctggatctcg aaactctggc
    54661 tgcggcactg ctgaaaatgg cacagggtga acgtactctg attgttccgc cagatgcgcc
    54721 gatgcgtccg aaacgtgaat tccgtgaccg tgatgaccgt ggtccgcgcg atcgtaacga
    54781 ccgtggcccg cgtggcgacc gtgaagatcg tccgcgtcgt gaacgtcgtg atgttggcga
    54841 tatgcagctg taccgcattg aagtgggccg cgatgatggt gttgaagttc gtcatatcgt
    54901 tggcgcgatt gctaacgaag gcgacatcag cagccgttac atcggtaaca tcaagctgtt
    54961 tgcttctcac tccaccatcg aactgccgaa aggtatgccg ggtgaagtgc tgcaacactt
    55021 tacgcgcact cgcattctca acaagccgat gaacatgcag ttactgggcg atgcacagcc
    55081 gcatactggc ggtgagcgtc gtggcggtgg tcgtggtttc ggtggcgagc gtcgtgaagg
    55141 cggtcgtaac ttcagcggtg aacgccgtga aggtggtcgt ggtgatggtc gtcgttttag
    55201 cggcgaacgt cgtgaaggcc gcgcaccgcg tcgtgatgac tctaccggtc gtcgtcgttt
    55261 cggtggtgat gcgtaatcat cgctgaacag cgaacacaat ctgtaaaata atatatacag
    55321 ccccgatttt aaccatcggg gctttttttc tgtcttttgt actcatgtac tggtacagtg
    55381 caatgcataa caacgcagtc gcactatttt tcactggaga gaagccctca tggcaacact
    55441 aaccaccacc caaacgtcac cgtcgctgct tggcggcgtg gtgattatcg gcggcaccat
    55501 tattggcgca gggatgtttt ctctgccagt ggtcatgtcc ggggcgtggt ttttctggtc
    55561 aatggcagcg ctgatcttta cctggttctg tatgctgcat tccggcttga tgattctgga
    55621 agctaacctg aattaccgaa tcggttcgag ttttgacacc atcaccaaag atttgctggg
    55681 caaaggctgg aacgtggtca acggcatttc cattgccttt gtgctctata tcctgaccta
    55741 tgcctatatt tctgccagtg gttcgattct gcatcacacc ttcgcagaga tgtcgctaaa
    55801 cgtcccggca cgggcggcgg gttttggttt tgcactgctg gtagcgtttg tggtgtggtt
    55861 gagtactaaa gccgtcagcc gcatgacggc gattgtgctg ggggcgaaag tcattacctt
    55921 cttcctcacc ttcggtagcc tgctggggca tgtgcagcct gcgacattgt tcaacgttgc
    55981 cgaaagcaat gcatcttatg cgccgtattt gctgatgacc ctgccgttct gcctggcgtc
    56041 gtttggttat cacggtaacg tgccaagcct gatgaagtat tacggcaaag atccgaaaac
    56101 catcgtgaaa tgcctggtgt acggtacgct gatggcactg gcgctgtata ccatctggtt
    56161 gctggcgaca atgggcaaca tccctcgtcc ggagtttatc ggcatcgccg agaagggcgg
    56221 taatattgat gtgttggtac aagcattaag cggcgtgctg aacagccgta gcctggacct
    56281 tctgctggtc gtgttctcaa actttgcggt agcgagttcg ttccttggcg taacgctggg
    56341 tttgtttgac tatctggcag atctgtttgg tttcgacgac tcggctgtgg gccgcttgaa
    56401 aacggcattg ctgacctttg ccccgcctgt tgtgggtggt ctgctgttcc cgaacggatt
    56461 cctgtatgcc attggttatg ctggtttagc ggctaccatc tgggcggcaa ttgttccggc
    56521 actgctggcc cgcgcatcgc gtaaacgctt tggcagcccg aaattccgcg tctggggtgg
    56581 caagccgatg attgcgctga ttctggtgtt tggcgtcggc aacgcactgg tgcatatttt
    56641 atcgagcttt aatttactgc cggtgtatca gtaatcagcg gtgccttatc cgacatttct
    56701 gctgcctaca caatgcctga tgcgcttcgc ttatcaggcc tacgtaggac agcgttgcca
    56761 gctcggataa ggcttcccgc gttaagacac actatcccaa caactcttcc ttaacatcca
    56821 tcgccagctc aaacgaatgc aggcgcgcct ggtggtcgaa aatctgcccg ttaaccataa
    56881 tctcatcagc gtcggtttcg cgcaggatcg actgcaaacc atgacgcact ttcgctttat
    56941 cgcctaccaa cgacatactc agcgcctgct gcacgtcata ctgctcagac ggtgaccaga
    57001 actgatccat attttgaatc ggcggcggca gttgcccggt ttcgccacgg cgcagcttca
    57061 caaaggcttg ctgcattgag gtaaacagaa attcagcgtc gcggttgctg tcggcggcga
    57121 taatattgat gcacaccatc gcgtatggtt tttccagccg agccgacggt ttgaagttac
    57181 tgcgataaag atgcagcgcc tggaacaaca tatccggcgc gaagtgtgag gcaaacgcaa
    57241 atggcagacc aagctgcgcc gccagttgtg cactgtaaag gctggagcct aacaaccaca
    57301 cggggatttt ctcgccatag cctggtaccg ggcgcacatg cggattggga tcgcgggcgt
    57361 caaaccagtc caccagctcc gcgacatcgc gggggaaatt atcaatatcg ccgctcatat
    57421 gacgacgtag cgccatcatt gtccgttggt cactacccgg agcacgaccc agccccaaat
    57481 cgattcgccc cggatagagc gtattgagtg tgccgaactg ttcggcaatg accaacggta
    57541 agtggttagg caacatcacg ccgccagacc ccagatgcag cgtggtggta ttcgccgcca
    57601 gatagccaat caataccgat gttgcagcac tggcaatgcc ggtcatattg tggtgttctg
    57661 ccagccagta gcgatgatag ccgcgctttt cagccagacg ggcgagatcg agagagtggg
    57721 agaacgcttc ccgcgctgaa gagccttcgg gtatgggggc cagatcgagt agcgaaaacg
    57781 caatggtttt atcagtcata acagctcact ttgttaccga ttaatcttta atactgcatt
    57841 aaagaatagt taaagttgtt aacaaagtga gctatttact taggcttgca gctccagtcc
    57901 tgccagccgc cgccagtagc cgttacagtc gctgtttgcc gtcaacggca gtggcacagc
    57961 gccattttca ttagcgcgga aggcgtcgag catcgcgaaa gtgtcagtgc cctgcggtga
    58021 caggcggact acatcaacca atccctgcat ggatgccagc tcgttaccga ggttgtaaac
    58081 gtagccgctc atggtctgaa tgccattgag tacaaacact tgttggtttt cctgcgacag
    58141 cacgttgcgc ccgttcggat acttaatgca gcaggtttca cactcatctt tcgggcggtc
    58201 ttccgaacgc gcggtaaagc agcgggcgga gtaggccagc ggtagatgac cgtagctcag
    58261 gacttccact tcaaactggt tgcgaatgcc cagctcatcg cactgattaa gcagattcac
    58321 cagccagtcg cgggaaagct ccaccggcat acaccagcgc atcatgccct gtttgagcaa
    58381 tattttcagc gtcaccgcgt tgtagcagtt cagcgcgtgc ccggcgacga acggcagttt
    58441 gcgttcagcg cacatattca ccacgccgag atcgctggct tcaatcagaa attcaccgtt
    58501 ctcaacatag cgtttcagtt cgcccagttc agaggatgcc tgcaccagcg ccagcgtgga
    58561 gagcacaatc tgcttgccac ttccggcgag cgattttgcc atctccagac agtcgccaac
    58621 tttggttgcc cggcgcttgc tgcataccgc ttcaccaaga taaatcacgt cggcgctgct
    58681 ggcggcggct tgctgataaa aatcttccag cgtctctttt ggccagtacc acagcactgg
    58741 ccctaaggaa tatttcattg ctcttctcac tgccatttac ggtgatacgc gccaagggtg
    58801 gtttgcgtgc cttcggacat cgacccgagc gtctccatcc acgcgctttg cggtacgaag
    58861 ttttgcggat cggccttaca acggtcgata gcctgacgcc agactttcgc cgcctggctg
    58921 acatacgccg ggctacgctg gcggccttca attttcaccg aagcaatatt cgcagccatt
    58981 aactccggca gcagttccag ggtattgaga ctggttggtt cttccagcgc gtgatagcgc
    59041 tcgccgtcca ccagataacg ccctttacac agcgtcggat aacctgcgtt ttcgccgtcc
    59101 tgataacggt cgatcagcac ttcgttcagg cgggattcca gcccctgcgg cgtttgttgc
    59161 cagcgcacga aacgggccgg agaacacgca cccacagtgt tgggcgactc acccgtcaga
    59221 tacgacgaca gatagcaacg gccttccgac ataatacaca ggctgccgaa agcaaacact
    59281 tccagcggga caggagtaac ccgtgccagc tgtttcacct gatgaatcga caacacgcgc
    59341 ggcagcacca cgcgggcaac gtcaaaatgg cgatgataaa agttaatcgc ctcttcattg
    59401 gtcgccgaag cctgcactga cacatgacgt tcaatatgcg ggtaacgctc ggcggcgtac
    59461 tccagcatgg cgaggtcggc gaggatcagc gcgtcggcac ccagctgcgc cgccatatcc
    59521 acggcacgct gccaacgggc gtaaccgtcc ggatgcgcaa aagtgttaat cgcgatgtga
    59581 agtttgcggc gatgttgatg gacaaaactc accgcttcct gcaatttttt ctcggtaaag
    59641 ttaaggccgg cgaagtgacg ggcattggta tcatctttta gcccgatata aacagcatct
    59701 gcgccgtttt cgatggccgc cttaagcgcc gggagatttc cggcagggca gagcagctcc
    59761 ataatttttc ctgaaggttg cgctatcaaa acgcaaaaat gttaacgaac tgggatttta
    59821 gttaaccttg ctgcgacaat ttttgattta aggcagttaa agttgtattg gtggtagcag
    59881 caatttcata
//
LOCUS       DAZSNM010000003        55461 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZSNM010000003 DAZSNM010000000
VERSION     DAZSNM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55461
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.003"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(602..2188)
                     /locus_tag="ACHW42_000112"
     CDS             complement(602..2188)
                     /locus_tag="ACHW42_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HFR8942112.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(2543..4714)
                     /gene="glcB"
                     /locus_tag="ACHW42_000113"
     CDS             complement(2543..4714)
                     /gene="glcB"
                     /locus_tag="ACHW42_000113"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFR8942113.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4736..5140)
                     /locus_tag="ACHW42_000114"
     CDS             complement(4736..5140)
                     /locus_tag="ACHW42_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HFR8942114.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRATKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5145..6364)
                     /gene="glcF"
                     /locus_tag="ACHW42_000115"
                     /pseudo
     CDS             complement(5145..6364)
                     /gene="glcF"
                     /locus_tag="ACHW42_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6375..7427)
                     /gene="glcE"
                     /locus_tag="ACHW42_000116"
     CDS             complement(6375..7427)
                     /gene="glcE"
                     /locus_tag="ACHW42_000116"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFR8942115.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7427..8926)
                     /gene="glcD"
                     /locus_tag="ACHW42_000117"
                     /pseudo
     CDS             complement(7427..8926)
                     /gene="glcD"
                     /locus_tag="ACHW42_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9177..9947
                     /gene="glcC"
                     /locus_tag="ACHW42_000118"
     CDS             9177..9947
                     /gene="glcC"
                     /locus_tag="ACHW42_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFR8942116.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10071..11212)
                     /gene="yghO"
                     /locus_tag="ACHW42_000119"
                     /pseudo
     CDS             complement(10071..11212)
                     /gene="yghO"
                     /locus_tag="ACHW42_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
     gene            11616..13304
                     /locus_tag="ACHW42_000120"
     CDS             11616..13304
                     /locus_tag="ACHW42_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFR8942117.1"
                     /translation="MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
     gene            13301..14215
                     /locus_tag="ACHW42_000121"
     CDS             13301..14215
                     /locus_tag="ACHW42_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR8942118.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14248..14496
                     /locus_tag="ACHW42_000122"
     CDS             14248..14496
                     /locus_tag="ACHW42_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR8942119.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            14496..15667
                     /locus_tag="ACHW42_000123"
                     /pseudo
     CDS             14496..15667
                     /locus_tag="ACHW42_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(15703..16782)
                     /gene="lptG"
                     /locus_tag="ACHW42_000124"
     CDS             complement(15703..16782)
                     /gene="lptG"
                     /locus_tag="ACHW42_000124"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR8942120.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(16779..17849)
                     /gene="lptF"
                     /locus_tag="ACHW42_000125"
     CDS             complement(16779..17849)
                     /gene="lptF"
                     /locus_tag="ACHW42_000125"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR8942121.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(17880..18440)
                     /locus_tag="ACHW42_000126"
     CDS             complement(17880..18440)
                     /locus_tag="ACHW42_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR8942122.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18452..19276)
                     /locus_tag="ACHW42_000127"
     CDS             complement(18452..19276)
                     /locus_tag="ACHW42_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942123.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19276..20625)
                     /locus_tag="ACHW42_000128"
     CDS             complement(19276..20625)
                     /locus_tag="ACHW42_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR8942124.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(20671..21429)
                     /locus_tag="ACHW42_000129"
     CDS             complement(20671..21429)
                     /locus_tag="ACHW42_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942125.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21461..22174)
                     /locus_tag="ACHW42_000130"
     CDS             complement(21461..22174)
                     /locus_tag="ACHW42_000130"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR8942126.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22348..23040
                     /locus_tag="ACHW42_000131"
     CDS             22348..23040
                     /locus_tag="ACHW42_000131"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR8942127.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23089..24588)
                     /gene="pitB"
                     /locus_tag="ACHW42_000132"
     CDS             complement(23089..24588)
                     /gene="pitB"
                     /locus_tag="ACHW42_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR8942128.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(24880..26739)
                     /gene="gss"
                     /locus_tag="ACHW42_000133"
     CDS             complement(24880..26739)
                     /gene="gss"
                     /locus_tag="ACHW42_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR8942129.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            26944..27810
                     /gene="yghU"
                     /locus_tag="ACHW42_000134"
     CDS             26944..27810
                     /gene="yghU"
                     /locus_tag="ACHW42_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR8942130.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(27839..27920)
                     /locus_tag="ACHW42_000135"
                     /pseudo
     CDS             complement(27839..27920)
                     /locus_tag="ACHW42_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27933..28181)
                     /gene="hybG"
                     /locus_tag="ACHW42_000136"
     CDS             complement(27933..28181)
                     /gene="hybG"
                     /locus_tag="ACHW42_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR8942131.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28315..28656)
                     /gene="hypA"
                     /locus_tag="ACHW42_000137"
     CDS             complement(28315..28656)
                     /gene="hypA"
                     /locus_tag="ACHW42_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR8942132.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(28649..29137)
                     /gene="hybE"
                     /locus_tag="ACHW42_000138"
     CDS             complement(28649..29137)
                     /gene="hybE"
                     /locus_tag="ACHW42_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR8942133.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29130..29624)
                     /locus_tag="ACHW42_000139"
     CDS             complement(29130..29624)
                     /locus_tag="ACHW42_000139"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR8942134.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(29624..31327)
                     /gene="hybC"
                     /locus_tag="ACHW42_000140"
     CDS             complement(29624..31327)
                     /gene="hybC"
                     /locus_tag="ACHW42_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR8942135.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31324..32502)
                     /gene="hybB"
                     /locus_tag="ACHW42_000141"
     CDS             complement(31324..32502)
                     /gene="hybB"
                     /locus_tag="ACHW42_000141"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR8942136.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32492..33478)
                     /gene="hybA"
                     /locus_tag="ACHW42_000142"
     CDS             complement(32492..33478)
                     /gene="hybA"
                     /locus_tag="ACHW42_000142"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR8942137.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33481..34599)
                     /gene="hybO"
                     /locus_tag="ACHW42_000143"
     CDS             complement(33481..34599)
                     /gene="hybO"
                     /locus_tag="ACHW42_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR8942138.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(34788..35075)
                     /gene="yghW"
                     /locus_tag="ACHW42_000144"
     CDS             complement(34788..35075)
                     /gene="yghW"
                     /locus_tag="ACHW42_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR8942139.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35194..36081)
                     /gene="yghX"
                     /locus_tag="ACHW42_000145"
     CDS             complement(35194..36081)
                     /gene="yghX"
                     /locus_tag="ACHW42_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR8942140.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36238..37278
                     /gene="gpr"
                     /locus_tag="ACHW42_000146"
     CDS             36238..37278
                     /gene="gpr"
                     /locus_tag="ACHW42_000146"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR8942141.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37318..37812)
                     /locus_tag="ACHW42_000147"
     CDS             complement(37318..37812)
                     /locus_tag="ACHW42_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR8942142.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38003..38887
                     /gene="yghA"
                     /locus_tag="ACHW42_000148"
     CDS             38003..38887
                     /gene="yghA"
                     /locus_tag="ACHW42_000148"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR8942143.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39159..39584)
                     /gene="exbD"
                     /locus_tag="ACHW42_000149"
     CDS             complement(39159..39584)
                     /gene="exbD"
                     /locus_tag="ACHW42_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR8942144.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(39591..40325)
                     /gene="exbB"
                     /locus_tag="ACHW42_000150"
     CDS             complement(39591..40325)
                     /gene="exbB"
                     /locus_tag="ACHW42_000150"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR8942145.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            40577..41764
                     /gene="metC"
                     /locus_tag="ACHW42_000151"
     CDS             40577..41764
                     /gene="metC"
                     /locus_tag="ACHW42_000151"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR8942146.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            41904..42563
                     /gene="yghB"
                     /locus_tag="ACHW42_000152"
     CDS             41904..42563
                     /gene="yghB"
                     /locus_tag="ACHW42_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR8942147.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(42603..43502)
                     /gene="yqhC"
                     /locus_tag="ACHW42_000153"
     CDS             complement(42603..43502)
                     /gene="yqhC"
                     /locus_tag="ACHW42_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR8942148.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            43696..44859
                     /gene="yqhD"
                     /locus_tag="ACHW42_000154"
     CDS             43696..44859
                     /gene="yqhD"
                     /locus_tag="ACHW42_000154"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR8942149.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            44964..45791
                     /gene="dkgA"
                     /locus_tag="ACHW42_000155"
     CDS             44964..45791
                     /gene="dkgA"
                     /locus_tag="ACHW42_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR8942150.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            45991..46917
                     /locus_tag="ACHW42_000156"
     CDS             45991..46917
                     /locus_tag="ACHW42_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR8942151.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            46968..47225
                     /gene="yqhH"
                     /locus_tag="ACHW42_000157"
     CDS             46968..47225
                     /gene="yqhH"
                     /locus_tag="ACHW42_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR8942152.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47268..49487)
                     /locus_tag="ACHW42_000158"
     CDS             complement(47268..49487)
                     /locus_tag="ACHW42_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR8942153.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(49598..51010)
                     /gene="ftsP"
                     /locus_tag="ACHW42_000159"
     CDS             complement(49598..51010)
                     /gene="ftsP"
                     /locus_tag="ACHW42_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR8942154.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51085..51822)
                     /gene="plsC"
                     /locus_tag="ACHW42_000160"
                     /gene_synonym="parF"
     CDS             complement(51085..51822)
                     /gene="plsC"
                     /locus_tag="ACHW42_000160"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR8942155.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52056..54314)
                     /gene="parC"
                     /locus_tag="ACHW42_000161"
     CDS             complement(52056..54314)
                     /gene="parC"
                     /locus_tag="ACHW42_000161"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR8942156.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(54860..55342)
                     /locus_tag="ACHW42_000162"
     CDS             complement(54860..55342)
                     /locus_tag="ACHW42_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR8942157.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55395..>55461)
                     /locus_tag="ACHW42_000163"
     CDS             complement(55395..>55461)
                     /locus_tag="ACHW42_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00156 family protein"
                     /protein_id="HFR8942158.1"
                     /translation="EVDKDWNSVEIDVKQIRKVNP"
BASE COUNT        13705 a        14853 c        13782 g        13121 t
ORIGIN      
        1 ttcttatttc taacaaaatg attggtgtgt tgttggagtt aaaatacata cataatatta
       61 acatctgcca acatttcact ttccgcaaga cggatgattt cgaatcctca tccacgttac
      121 cctatcccaa atatctttat taataggcag taacgaacac tgttatagca agattaactt
      181 attaacctaa cggcatcttt tatgtaacaa gtggagataa attgcttaaa attgatcctc
      241 gacaaatctt aaaagttctc attattctgc aaaatcagct ttgcgaataa tcttatgttt
      301 ttgggagttt tgggaatttt gatctactca ttgttctagt gaaaaatata aataagagaa
      361 aaaacgccat tcgtcatgaa agcaacagat gaagcaatgt aataacatac taaaattgat
      421 ctcatgaaag tgggtacttt ataaccagca cagaagccaa gttgtataaa tattaaacaa
      481 ttcgcatcgt aaaacggaga ctacgtataa agaagccaat cgtaaaaacg gttccctggc
      541 gagataaacg acagacaaat ggaaagccgg gtgatattta ccttcatccg gctttattga
      601 tttacgagac taacatcccg gtaaacacat acgcctgcag cagggtgata acgctggcaa
      661 aaatcagact gtgcttcacg gtgtagcgga acagttcaga ttctcggccc accatgcccg
      721 tcgcggcgca ggccacggcg atagattgcg gggagatcat cttgccagtt acgccgccgc
      781 tggtatttgc tgccaccagc agagtgtcag agacgttgat ttgctgcgcc gtggtcgatt
      841 gcagtgaacc aaacagggcg tttgaagaag tgtccgaccc ggtaaggaat acgcccagcc
      901 agccgagaaa cggtgagaaa aacgggaaca tcacgcctgt acctgccagt accagcgcca
      961 gcgtggtgga catgccggaa tagttggtga cgaaggcgaa tgccagcacc atgccaatcg
     1021 acaatatcgg ccacttcaga ctaattagca tttcggcaaa gacgccaatc cctttcttga
     1081 tgcccacgcc gaggatgaag atagagataa tcgccgcaat aaaaatagcg gtgccgccag
     1141 ccgagagagg gtcgaattta aacaccgcat ccattggcgt tggttgggcg acaatgggtg
     1201 ccgctttcaa cacttgttga tgcaaatgag ggatctggaa attaatcacc agtgaataaa
     1261 acgcgccgcc cggagcaaat aacgctttaa acggcttcat ggtccagatg gtgaccagca
     1321 ccgttaagat taaaaacggt gaccacgcac gaatgatttg ccccagacta tattctgaag
     1381 gcacgggacc gccagaagat ggcttattta ccaccatcgc acctgcggat tgccccatgc
     1441 tgattgccgt ttcggtattt ttcggccgcc agactttaag gaataaagcg agtgagacga
     1501 tactcaccag cgccgaagta atatccggca gttccggacc aatatagtta gaggtaaaga
     1561 actgagtgac agcgaagctt cccccagcaa ccagcgccgc tggccacgtc tctttcaccc
     1621 ctttccagcc gtccatcatt gctaccagcc agaacggcac aagaaccgac aggaacggta
     1681 actgacgtcc cgccattgcg ccaatgtgga acggatcgat tcccgttacc tgaccggcga
     1741 ccagaatcgg cacgcccaac gcaccaaacg ccaccgtcgc accaaagcca gcagctcctt
     1801 ccagcaacgc accaaaggag aaaccaatca gtaacacctg caaacgctga tcgtcggtga
     1861 tggagataac cgagctgcgg ataatatcga actgcccgct ggcaacggtt aatttataca
     1921 ggaacaccgc cgcgacaata atccacgcaa ttggccataa tccataaata aagccatagc
     1981 ccgcagcagc aaatgccata tcaatcggca ttttaaaggc gaatatcgca atcaggatag
     2041 ataatataag ggttattgct ccagcaacat gtcctttcag acgtaatacc gcgagtgcaa
     2101 cgaagaagaa tattatcggg atcagggcga ccagagcgga tagccccagt cctcccatcg
     2161 gcatatacat ttgggtccag gtaaccatat tgttcagtct cttattatct ttatatgctt
     2221 gatatactta aggttgtaat aagcaaaaga ggactgaact gtaaaatata ggcgttatac
     2281 tttacagcaa cagtacgccg ctaacgcaat tgctacctct ggcataacaa gtatatcggg
     2341 taagggtttc tgttccgcac acgcaggcgc agagtatcgt taagatgtcc atattgttgt
     2401 tttaggcccg ctagtaatgc gctacgggta tttaatattg ttaaaccctg ataatcgctc
     2461 cggttatttc cgggataaat gtactaccgc agttactatc atagccccga caataaaact
     2521 tgccggggct tttttgacgc tattaatgac tttctttttc gcgtaaacgc caggcgtgta
     2581 ataacggttc ggtataaccg tttggctgtt tcacgccgag gaagattaaa tcgctggcag
     2641 ctttaaaagc acacgagtta gcgaaattcc ccgccatcgg acgataagcc ggatcgccag
     2701 cattttgctg atcaaccact ttcgccatat tctccagcga cgcctgcacc tgttctttgg
     2761 tcagaatacc gtgacgtaac cagttggcga tatgctggct ggagatacgc agcgttgcgc
     2821 ggtcttccat cagcgccacg ttgtgaatat ccggcacttt tgaacaacca atcccctgct
     2881 ccacccaacg caccacgtac cccagaatcc cctgcacgtt gttatccagc tcttgttgga
     2941 tctcttgcgc cgaccagtta gcgttttcag ccaccggaat agtcagcaga tcgtccagca
     3001 gcggttcaaa ttcagcactg aactcggtct gggcaatgtt ggcttgcacg ctctgtacgt
     3061 tggtatggtg gtagtgcagc gcatggagcg tagcagcggt tggtgacgga acccaggctg
     3121 tgtttgcccc ggcacgcagt tggtcgccct tctggctgta catgtctgcc atcaggtccg
     3181 gcattgccca catgccttta ccgatttgcg ctttaccgcg cagcccacag aacagaccgg
     3241 aaagcacgtt attacgttcg taggctttga tccacggcgt cgatttcatc tggtttttac
     3301 gcagcatcgg gccagcttcc atcaccgaat gcatttcatc gccggtacgg tcgaggaaac
     3361 cggtattgat gaacgccacg cggttgcgcg cctgagcgat acagctacgc aagttcagcg
     3421 aggtccggcg ttcttcatcc ataatgccca ttttcagggt attcggtgcc ataccgagca
     3481 ttgtctcaat gcgggtaaac agtttgttgg cgaacgccac ttcctgcgga ccgtgcattt
     3541 tcggtttcac aatatagacg ctgccagtgc gcgagttttt ctgcactttt aaatcataga
     3601 gggcaatcgc gccagtcatg acgccatcaa gaataccttc cgggatttca ttgccttcgc
     3661 tgtcccaaat cacaggaatg gtcatcaaat gacccacgtt gcggataaac agcaacgagc
     3721 gtccgtgcag agaaatttca gagccatcgg cggcggtgta ctgacgatcg tcattcagtt
     3781 tacgtacgat ttgccgaccg tttttctcca ttttctcttg cagagtcccc tgcatcaggc
     3841 ccagcagatt gcggtacagc aggattttat cttccgcatc aaccgccgcg accgaatctt
     3901 cgcagtcgag aatggtactg atagcagctt cgacgataac atcgttgatg tgcgccggat
     3961 cgtctttgcc aatccgcccg ttggcatcaa tttgcagttc aatatgcagg ccgttatttt
     4021 tcagcaaaat gcaggtcggc gcagcggcat cgccacggta accgacaaac tgtgctggag
     4081 tacgtaacgt ggtttcttta ccatttttca actggatgcg taattgttta tcgaccacct
     4141 taaacgccac cacatcctga tagctgccgt tttccagcgg tagtgattca tcgaggaaac
     4201 gtcgaaccca ggcgataacc tgctcaccgc gctgcggatc gtagccactg accatcgccc
     4261 cttcctgcgg gatgatgtcg ctgccgtata acgcatcgta cagtgagccc cagcgagcgt
     4321 tcgccgcgtt cagcgcgtag cgggcgttca ttgccggaac caccagctgt ggccccgcct
     4381 gactggtgat ttcgctgtca atgcccgtgg tttccaccgt cacgcgctcc ggttgcggca
     4441 ccaggtagcc cagttcacga aggaaagatt tataggccgc tttatctttt accggccccg
     4501 gattgctgcg atgccactca tcaagcgccg cctgaatgcg atcgcgttct gccagcaact
     4561 gacgattttc tggtgccaga tcgtgaacga tctcatcaac attgtgccag aatgcagcag
     4621 cgtccagttc cacgcccggc aaaacttctc catccacaaa acgtttaaaa ttggcgtcaa
     4681 tgcgtaaacg gccctgggtt atggtttgac tcattgtttc tctcctcgtt ttcgcttatt
     4741 tcgccaacac cgctgctgcc actttcgcga cctgcgcatc ctgtgctccg gttaaaccag
     4801 aaacgcccac ggcaccaata atttgcccat caacaacaac cggtacgccg ccttccagcg
     4861 acgtcagtaa cggcgcagtc acgaacgcgg tacgtccgtt gttcaccatc tcttcatagc
     4921 ccttagttgc acgacgcccc agcgcggcgg tacgcgcttt ctcctgggag atgtaagccg
     4981 caatcggcgc gcaatcgtcc atgcgactta acgccagcag atgaccgccg tcatcggcaa
     5041 cagcaatgga aacagaccag ttatttttct gcgcctcttc ctgacctgcg gcaataattg
     5101 cactcgccat ttgctggcta agaatgactt tagttttcat ttttttattc cttttcaagg
     5161 gcttgttcta caatttcaat ccagtgacgc acagaggtac gaccggcgct cgccagatgc
     5221 gtctggcaac caatgttggc ggtgacgatc atttccggtt tgccgctttc cagcgcattc
     5281 attttgttat cccgcagctg gcgtgccaga tcgggatgcg ttaacgcata tgttcccgct
     5341 gaaccgcagc acagatggct gtcgggaacg tccgttaagg taaatccaag acgaagtaac
     5401 actttttcca cttcgccgtt cagcttttgc gcatgttgta gggtacacgg gcagtggaag
     5461 gccagttttt tatcgccgcg aattgccagt ttttccagcg gttcctcgcg cagaagttcg
     5521 actaaatcga ccgccagttc actgacctga cgcgctttat cggcatataa cgcatcgttt
     5581 ttcagcatct gcccatactc tttgacgaac gcgccgcaac cgctggcggt ttgcaaaatt
     5641 gcctcggcac ctgcttcaat cgcgggccac caggcatcaa tattattgcg cgcccgtgcc
     5701 aaccctttct cctgcgcatt aagatgatag tccaccgcgc cacaacagcc tgcttcgtta
     5761 gctgacatga cgctgatccc cagacgatcc agcactcgcg cagttgccgc gttggtgttg
     5821 ggcgaaagcg taggctgggc gcagccttcc agcattaaaa cccgacgctt atggcgcagc
     5881 ggcggacgcg gtttagcttt caccgtttca gcaggcagtt ttgctctgac ctgttccggt
     5941 aaaaacggtc gcagcaccag ccctacctgc gtcagcgcac ggaagaccgc cggacgcggc
     6001 actacctggc gcaatccttc gcgcagcatt cgctccggca gtgggcgttt cactttctgc
     6061 tcgacaatat cacgcccgat atccagcaaa ttgtgatagc gcacaccaga aggacaggtg
     6121 gtttcacaat tgcggcaagt gaggcagcga tcgagatgct cctgtgtttt aagcgtgact
     6181 tcgttgcctt ccagcacctg tttaatcaga tagatgcgcc cgcgcggccc gtccagttca
     6241 tcgcccagaa gctgataggt tgggcaggtt gcggtacaaa atccgcagtg aacacaggcg
     6301 cacaggatgc tgtcggcttc cagcgcgcgt tctgccgcat ctcttcggtt aatcgggttt
     6361 gcatagcctg ctcctcaaag ttccgcgtac atgcgaccgg ggttaaacac gccgcaaggg
     6421 tcgagctgct gtttaagctg ctggtgatag cggaataaag gagccgatag cggggcaaag
     6481 ccaccatctc cggcactaaa gcgggtcgca tgaccgccag cgttgcgggc gatgcgatgg
     6541 atttgattgt catcggctgt cgatttcagc cagcgtaacg ccccgcccca gtcgatcagt
     6601 tgctcgccgg gtaaatccat catcggcgca tcgctgggta atgaaatgcg ccataaggta
     6661 cctggtaacg agaagaacgg cagttgttgt tcacgcaatt gctgccagaa ctgacccaca
     6721 acctcttcgc cacccagcag ttcacgcgct gcttttaccg atccttcgcc gccctcaagg
     6781 cggatccaca acgcattgtc gaagtaacat aagccactaa tgggtaatgg ctggagttgc
     6841 cactcggcga tttcactcat ggcttcttgc aggctgattt cccgacgcag gctcagagag
     6901 gcacgcggtc gcggtaacac tttcattgag atttcagtga gcacgccaag acaaccgtag
     6961 cttccggcca ttaaccgtga gagatcgtat ccggcaacgt ttttcatcac ttcgccacca
     7021 aaacgcagat gttttccagt gccggtaatg atgcgcgtgc cgaggacaaa atcgcggacc
     7081 gaaccgctcc acgggcgacg cggccccgcc agcccgcagg cgaccatccc gccccaggtg
     7141 gcttcttcac cataatgcgg cggctcgcag ggaagcattt gcccggcgct ctccagcgcc
     7201 gcttcaatcg ccaccagcgg cgttccggca cgagcggtta tcaccagctc ggtcgggtcg
     7261 taattaacaa tgccgcgatg acaacgcaca tccagcgttt gcccggtgac tgggcgacct
     7321 aaaaaggctt tgctattgct gccctgaatc accagcggcg ttttatcgct aattgcctga
     7381 ttcacctgct ccagcagcgc ctgactgtaa tcacactcgc gtagcatcaa aagcgctcca
     7441 gttcaggaaa aggtaaatga ccgtgatgca catgcatggc accaaattca gcacagcggt
     7501 gtagcgtggg aatgtttttc ccagggttca gcaaaccatc ggggtcaaac gccgccttga
     7561 ccgcatggaa ggtcgtgatt tcatcgctgt tgaactgggc gcacatttga ttgatttttt
     7621 ctcgcccgat gccatgttcc ccactgatgc tgccgccaac ttcaacgcag agttcgagga
     7681 tcttcccgcc cagctcttcc gcgcgggcaa attcaccagg ttcgttggca tcgaaaagga
     7741 ttaacgggtg catgttgcca tctccggcat gaaagacgtt ggcaacacgt aaatcatatt
     7801 gctgcgataa acgggcaatg ccttccagta cgccaggcag ggcgcgacgc gggatggtgc
     7861 catccatgca gtagtaatcc ggggagatac gtcctaccgc cgggaacgca tttttgagac
     7921 cggcccagaa acgtacgcgc tctgcttcgt cctgtgccaa acggacgtca gtcgcgcccg
     7981 ctttcaacaa gatgtagtta acccgctcgc agtcttcctg tacgtcagac tccacgccgt
     8041 ccagttcgca taacaaaatc gcttcggcgt cgacgggata accggcatga ataaaatctt
     8101 ccgccgcgcg gatcgacagg ttatccatca tctccagccc gccggggata atgccattgg
     8161 cgatgatgtc accaaccgca agtccggctt tttctaccga gtcaaagctg gctaacagaa
     8221 cccgcgccac gggcggcttc ggcagcagtt ttaccgtcac ttcggtggtc acgccgagca
     8281 taccttccga tccggtgaac agcgccagca ggtcaaaacc aggtgaatcc agcgcgtccg
     8341 atcccagcgt cagcgcctcg ccgtccagcg tttgcacttc aattttcagc aggttatgta
     8401 cggtcagacc atatttcagg cagtggacgc caccggcatt ttccgccaca ttgccgccaa
     8461 ttgaacaggc gatttgtgag gaaggatccg gcgcgtaata gagattatgc ggcgcaacgg
     8521 cctaggagat cgccaggtta catacgcccg gctgcacgcg cgcgcggcga ccaacggggt
     8581 taatgtcgag gctctcttta aagcgcgcca tcaccaacaa cacacctttt tccagcggca
     8641 gcgcgccacc agaaagcccg gtgcctgcac cacgggtcac caccggtaca cgcaggcgat
     8701 ggcagacagc cagaatcgct gtcacctgtt ccatttgctt aggcagaaca accagtaatg
     8761 gacgcgtgcg atacgcgctc aacccgtcac actcgtaagg aatgatctcc tcatcggtat
     8821 gcaggatctc aagtccaggg acatgctcac gcagtgccat cagtaccgat gtgcggtcga
     8881 catcgggtaa agcgccatca agacgctctt cgtacaagat gctcatgagt aggcttcgct
     8941 ttgttgtgtt gtgtggcagc tgatttttgc gcgctgcttc tgtgaacagt tattaagcgg
     9001 gcttttcgtt ttcgtctatc tctttagcta ccggtcagac catttttttt ccagctctgt
     9061 gaccttgtct tggttaactt aatgttaaat tgatgtaaca taatcacttg cgtgatgtgc
     9121 gtgttttgcg agttaagaac agaaaaattg gtcctacctg tgcacgaggt ccgggaatga
     9181 aagatgaacg tcgccctatt tgcgaagtgg ttgcagagag tatcgaacgg ttaattatcg
     9241 acggcgtact gaaggtcggt cagccgcttc cctcggaacg tcgactgtgt gaaaagctcg
     9301 gcttctcacg ctccgcactg cgtgaagggc tgaccgtgct gcgcgggcgc gggattattg
     9361 aaacggcgca gggtcgcgat tctcatgtcg cacggcttaa tcgggagcag gacaccagcc
     9421 cgctgatcca tctgttcagt acgcagccgc gaacgctgta cgatctgctc gacgttcgcg
     9481 cattactgga gggcgaatcg gcaaggctgg cggcaacgct gggaacgcag gctgattttg
     9541 ttgtgataac ccgctgttat gaaaaaatgc tcgccgccag tgagaaccac aaagagattt
     9601 cgctgatcga acatgcgcag ttggatcacg ctttccatct cgccatttgt caggcttctc
     9661 acaatcaggt gctggtgttt acgctgcaat cattgaccga tctgatgttt aattcagtgt
     9721 ttgccagcgt aaataatctc taccatcgac cacagcaaaa aaagcagatc gatcgccagc
     9781 atgcgcggat ctacaacgcg gtgttgcagc ggctgccgca cgtcgctcag cgcgcagcgc
     9841 gcgatcatgt acggaccgtg aaaaagaatc tccacgatat cgagctggaa ggccaccatt
     9901 tgattcgctc ggcggtgccg ctggagatga acaaggtggg aatgtgagca gagttttacc
     9961 ctatccccaa ggcgagggga ccgcccctgc acttaacagg atctggtgtt aaatctgttc
    10021 atctataccc tctcccgaat gaccgcccct gcgctaaaca ggatctggta ttaaatttgc
    10081 ttttcataca atcggtaacg cttgtacggc tccgccccaa tgcgttccag catgttattc
    10141 atgcctgtat tggtttcgag gatccatgac atctccagcg catcgatctt ccggcgggca
    10201 aacggatcgc gtaaggcttc aatcaataac agcgcaatca ccgggccgat gcggctgaac
    10261 tgatactcgt cgcgcacgcc catcagcggt actcgcgcag ttcgcacacc gctgactttc
    10321 aaacgccaca gcaattttgc ccagccgaag ggaaagagcg atccgttcag atcggcaatc
    10381 gcctcgttga tgttaggcaa gccacaataa acgcgcaggg tgcagaatca atctcagcga
    10441 tgtagatcat atcgtccggc accagatatt taagttggtc gcccatggtc gcgaattcat
    10501 gttcggtaaa cggcacaaat ccccagttgt gctgccagcc agagttgaaa atctcacgca
    10561 ggatctgcat ctcttcggca aaccgctgac gattgatgca gcgaatggtc acctttttgc
    10621 gcacctgatc catcagtttt tttagcgccg gagagaaagt gagatcggtt cgctgcatcc
    10681 accacgccag taaatcaatg cctttgtgat aacccagttg ttcaatatgc gcggcatacc
    10741 acggtttgcc gtgtggcatc atcgcacagg gtggtgtgtc aaaaccttca atcagtaatc
    10801 cgctttcctg attgatattc aggctgaaag gcccgctgat cttacttgca ccttgtgact
    10861 tcaaccacgc ttccgctgcg ccaaacaacg cggcaaaatc ctgcggatca tcaatggcgt
    10921 caatcatgcc gaaatgaccg gtatcttcac cgtaacgctc acggtgcaag gtatctattt
    10981 gcgcggtaat acgcccaact atctgccccg ctttttttgc cacccacgcc tgccagatga
    11041 tatggtccgt ccccggattt ttcgcagaca aatgctcgtt gcgttcaatg aataaagggg
    11101 gtatccagtt tggatcgtcg ggataaagtg atgacggaaa agcgataaat gccttaaggt
    11161 catttttatt aattactttt tcaattttaa tcatcagcag atcgcattcc atatctctgt
    11221 ggataacaaa taattaatgg tgaaaaacag gttgcaacag caacgagcgg catcttaata
    11281 atcaactcaa tgaatacaaa cgacgaataa atagtattaa ttaatgatta tgataaggtc
    11341 agaggctaca cagaaacgcc tcaatgcctt ttgtcaattt aaggcgaccg gacaaggctt
    11401 cttcttaata tattcataaa ttaaatatca ttcataataa aaacaaaaaa cttaaactca
    11461 ttatttaaat caatgttcat tttcacaccg atgaaaatac tatctcattt atcttttatg
    11521 cctatataat ccaccgcatt attgtcattt tatttttgat atccaatttt ttcgtggtgt
    11581 atatgtctaa taaaatcttt acgcattccc tacctatgcg ctatgccgat tttccaacgc
    11641 tggttgatgc tttggactac gccgctctga gtagagccgg aatgaatttt tatgacagac
    11701 gttgccaact tgaagatcaa ctggaatatc agacattaaa aacgcgtgcc gaagctggtg
    11761 cgaagcggtt gttatcgctg aacctgaaaa aaggcgaacg cgtggcgctg attgccgaaa
    11821 cgagtagcgg gtttgtagag gctttttttg cctgtcagta tgctggctta gtcgccgtcc
    11881 cgttggcgat tccaatgggc gtcggtcagc gggattcctg gagcgccaaa ctccagggtt
    11941 tactggcaag ttgccagccc gcagccatta tcactggtga tgagtggttg ccactggtca
    12001 atgccgcgac gcataacaac cccgaattac atgttttaag ccacgcttgg tttaaggcat
    12061 taccggaagc cgatgttgcg ctccagcgtc cagttccgaa cgatatcgcc tacctccagt
    12121 acacctccgg cagcacccgt tttccccgtg gcgtcattat cacccatcgc gaagtgatgg
    12181 ctaatctacg tgctataagc cacgatggca ttaaattacg ccctagcgac cgctgcgtct
    12241 cctggcagcc tttctaccat gatatgggac tggtcggctt tctcctgccc cccgtcgcca
    12301 cgcaactttc agtagattat ttgcgcactc aggattttgc catgcgtcct ttgcaatggc
    12361 ttaaattgat cagtaaaaat cgcggcaccg tttccgttgc gccgccgttt ggctatgaat
    12421 tgtgccaacg ccgcgtgaat gaaaaagatc tcgctgaact ggatctttcc tgctggcgcg
    12481 tcgctggtat tggtgctgag ccgatctccg cagaacaact ccatcaattc gctgaatgtt
    12541 tccgtcaggt taactttgac aataaaactt tcatgccgtg ctacggactg gcagaaaatg
    12601 cgctggctgt cagcttctct gatgaagcct ccggggttgt ggttaacgaa gtggatcgcg
    12661 acatcctcga atatcagggt aaagccgtcg cgccgggtgc agagacacgc gccgtatcga
    12721 ctttcgtcaa ctgcggcaaa gcgttgccgg aacatggtat tgaaatccgc aatgaagcag
    12781 gtatgccggt cgcggaacgt gtggtaggcc atatttgcat ctccggtccc agtctgatga
    12841 gcggttactt tggcgaccag gtttcgcaag acgagattgc cgcgacgggc tggttagaca
    12901 ccggcgacct cggttatctg ctggacggtt atctgtatgt caccggacgc attaaagatc
    12961 tgattattat tcgtggccgt aatatctggc cgcaggatat tgaatatata gcggaacagg
    13021 aaccggaaat tcattctggc gatgcgattg cttttgttac cgcccaggaa aaaatcattt
    13081 tgcagatcca gtgtcggatc agcgacgaag aacgtcgcgg gcagcttatc cacgcgctgg
    13141 cggcacggat ccaaagcgaa tttggcgtga ccgcggctat cgatctgttg ccgccccaca
    13201 gtattccccg aacgtcctcc ggcaagcctg cccgtgcgga agcgaaaaaa cgttatcaga
    13261 aggcttatgc tgccagtctt aatgtgcagg aatccctggc ataaatcaaa ctgtcgcggt
    13321 gacgggcgct accgggttta tcggtaaata tattatcgat aacctgctcg cccgcggctt
    13381 tcacgttcgc gcattgacgc gtactgcccg cgctcacgtc aatgataatc tcacctgggt
    13441 gcgcggttcg ctggaagata ctcattcact tagcgagctg gttgccggag ccagcgtagt
    13501 ggtccattgc gccgggcaag tgcgcgggca caaagaagag attttcaccc gctgtaacgt
    13561 tgacggcagc ctgcgcctga tgcaagcagc aaaagagagc ggcttttgcc aacgttttct
    13621 gtttatctct tcgctggcgg cgcgccatcc cgagctctcc tggtatgcaa aatccaaata
    13681 cgtcgccgag caacgactgg cagcaatggc ggacgaaatt acgctgggcg tttttcgccc
    13741 gacagccgtg tatggtcccg gcgataaaga gttaaaaccg ctgtttgact ggatgctacg
    13801 cggcctgctg ccacgacttg gtgcgccaga tacacaactc tcttttcttc acgtcaccga
    13861 ttttgcgcaa gcagtgggtc agtggttaag cgccgaaact gtacagacgc aaacctatga
    13921 attatgcgat ggcgtcgccg gtggctatga ctggcaacgc gtacgacagc ttgccgccaa
    13981 agcccgttgt ggttccgtgc gaatggttga cattcctctg ccggtactca caggccttgc
    14041 ggatatcagt accgcgttga gtcgcctggc aggtaaagaa cctatgctga cccgctcgaa
    14101 aattcgtgaa ttaacccacg ccgactggtc ggcaagtaat aaccgtattt ctgaagatat
    14161 caattggttt cccgggatta gcctggaaca cgcattacgc aacgggctat tttgattgtc
    14221 ttgttttaaa ggtgtgttaa cgtttctatg gtaaatcgtg aaatagtaat ggattacatc
    14281 ctttcctgtt tacaggattt ggtcgaaaac ggtgtggaaa ttaagcccga cagcgatctc
    14341 gtcaacgatc ttggcctgga gtcgatcaaa gtgatggatc tcttaatgat gctggaagac
    14401 agattcgata tttccattcc tattaatatt ctgctggatg tcaaaacccc tgcgcaatta
    14461 atggaaactc tacttccatg gctggagaat aaataatggg gctatacgat aaatatgcgc
    14521 gccttgctgg cgagcgtctg caatttagtg acaacgggtt aacaccgttt ggcacctgta
    14581 ttgatgaagt gtattccgcc accgaaggcc ggatcggcaa taaaaaagtg attctggccg
    14641 ggaccaataa ttatctcggt ttaaccttta atcatgatgc catcagcgaa ggtcaggccg
    14701 cgttagcggc ccaaggcacc ggcaccaccg gttctcgtat ggcaaacggc agttatgcgc
    14761 cgcacctggc gttagagaaa gagattgccg agtttttcaa tcgccctacc gctatcgttt
    14821 ttcaaccggt tataccgcta atttaggcgt catcagcgca cttgccgacc ataacgccgt
    14881 ggtgttactg gatgccgaca gccacgccag tatttatgac gtctgttctc tcggtggcgc
    14941 ggaaattatt cgtttccgcc ataacgatgc taaagatctg gaacggcgca tggttcgcct
    15001 cggtgaacgg gcgaaagagg cgattatcat cgttgagggc atctacagca tgctcggtga
    15061 cgttgcgccg ctggcggaaa ttgttgatat caaacgtcgt ctcggtggtt atttgattgt
    15121 cgatgaagcg cattcgtttg gcgtgttagg cgcaacgggg cgcggactgg ctgaagctgt
    15181 cggcgttgaa gatgatgtgg atattatcgt tggtactttc agtaaaagtc tggcctcgat
    15241 tggcggtttt gctgtcgttt cggaagcgat ggaagtgctg cgctacggca gccgcccgta
    15301 tatttttacc gcctctcctt ctccgtcctg cattgcgacc gtgcgctcgt cgttaagaac
    15361 tattgccaca caaccagagc tgcggcaaaa gctgatggat aacgccaacc atctgtatga
    15421 cggtctgcaa aaactgggtt atgagttaag ttcacatatt agcccggtag tgccagtaat
    15481 aatcggttcg aaagaggaag gtttacgcat ctggcgcgag cttatctcgc cgggcgtgta
    15541 tgtgaatctt atcctgccgc ctgccgcgcc tgcaggcatc acgctgctgc gctgtagcgt
    15601 aaatgctgcc cacagtcgtg agcaaatcga tgccatcatt caggcatttg ctactctgaa
    15661 gcagtaatgt gagcccgccg tggtgtttgc gccacggcgg gtttaatgtt gtctgtcata
    15721 ccaatacacc aggctcaacg ccaccaacac aaacgctacc ggtaaaccga gcgcggtaac
    15781 aggtgcgctt aacgcaaata gcaatcccag attaaccatg atttgatagc tgatccaggt
    15841 gagtaagccc acgatgacgc ctacagcgag acggctaccc atcccaggcg aacgcggggc
    15901 actaaaggta aacggcacag ccagcaaaat catcgccagg gtcaggatgg gttgccccag
    15961 tttttcccac agtgcaaggt ggtattcgct gctgggttgc ccggtttcct gcaaataatg
    16021 gatgtaatga ttaagctgac ggacagaaaa agtattcccc ggcattgata actcttcaag
    16081 attcatactg gtgaaggccg attgccaggc aagattatct aatgtttcca gcgtttcttt
    16141 gccattcagc cattttttat gattcacacc atgcaggacc cacgttttgt cgtcttcaat
    16201 ggtggcactg cgtgcataaa tgtaggattc aagcgagaga tcgtcgcgat aatgaaatat
    16261 ctccacgccc accggctggc cctgctcgtt cagcgatttc accgtcacaa attcattacc
    16321 acgcctggcc cacagcatat tgccggtaat gtcatcgtcc tcccccaacg cggtggcggt
    16381 cgatttgatt tgcaacgcct gttgctgcaa tggcgacgcc acccactcat ctatcgcgcc
    16441 taacgaaaca gtccacaata tcccggcaac cagcgccacc agtgcaatac ggaagataga
    16501 aaaccccgtg ctgcgaatgg cggtaagctc actgtttttc gataactgcc ccagtccaac
    16561 aataccaccc aataacgcga taaacggcga aagttcgacc agtgtgcgcg gtaaggtcat
    16621 tagcaccacc agcaccgcct gagtccagcg ataaccgccc gggctgacac catccagttc
    16681 gttaatcagg ttgaaggtgg taaaaagcgg cagcaatagc cctgcagcgg cggcaaaacc
    16741 gagaaagaga tgacggatta aatagcgact gaaaacattc atcgggaaaa tttctgcagc
    16801 aaagagaagt cacgtgcgac cagtatcagc aggcccatta gcatgagtcc gggcactaac
    16861 cacaaaccag gaatgagggg aatcgcgccg ttagccacca gtgtacggca gacgtcgccg
    16921 ccataaaaaa tggcaataaa cagcaacgtc agtggcaata acgttgaaaa tcgcccttgc
    16981 cgcggtttta cccgacttaa tgaaatggcc agcagcgcca tcaacaatgc ggtcaggcca
    17041 cggctttgtc gccattgcag ttcggcatga tcggcaggaa aggcggagcg cgccagctcc
    17101 gtgaccgatt tcgctttgcg tttgacgttc gggctttgat ccagcggatt cagatgtaat
    17161 tgcagattac ggtagatctg ttcgttgtcg tcacgcccct gatgatcgag aagataggca
    17221 gtgccgttgt gcaacataac ggtcggtttc tcgggtgatg ggtcaaccac atcaaccgaa
    17281 ccggcgcgga aaatacgggt tcgattggca gtagaagtat aaatcagcgc gtcagtcagg
    17341 cgattattat cctgatcaac cgtctgcgaa aggatcattc gtccgttatc gttagtgtta
    17401 aatttctttg cccgcaactg gcgaacatcc agctccgact gcgactgctg ctccagttga
    17461 taaatctgcg cataagccca cggacgacca tacatcgaca gaagggtgac aaaaatactt
    17521 agcggtatcg ccagataaag aacggctttg tacaagcgtc ccggactgcc ccccgccgca
    17581 gagatagcgg taatttccga gtcggtatac atctgcccta gcgtcacgcc aactgacaca
    17641 tacagaccaa caggtaacaa catctccagt gcaatcagta ctttgtagaa aacgatatcc
    17701 agcacaacat caagagctaa tgtcccattt gccgcttcgg tcagataacg ctgtgcggag
    17761 tagctggcga aaataaagat cagaaaaccg acgataatca gcaccagacg gcgcgtacca
    17821 cgcatgatgt agtgttcaac cagcttcatc tcactaacgc cctcaatcct ttgagttttt
    17881 tattcattga tcagtttctg cgccaacgca atgacatgat ctacctcttc cggcgtcagg
    17941 ctgccgtcct ttgcccactg aatttgcccg gctttattca gcaccagaat ggttgaactc
    18001 tgctccggca agcgccaggc aacgcgtccc tgtccattgc cgtcaataac gaattgcgcc
    18061 cacggatagc ggcgtttatt tttttcgatt ttgccaacaa caaaatagcc agtaccaaag
    18121 atggcatcat cggtgttcac aatggtggtg ggctgaaaac gatcctgcgg aaagtttgcc
    18181 gcttcaaccg ctttaattaa cagtgagttc tttttcttcg ccgacttacg cccggcgata
    18241 tactgaataa tgcggacttt tcccgctaat tgtgtgctgt tccacttttg gtagtgaatt
    18301 tcgtcgtcgc tattcagaag cagctcgccg ccttcctgga tataaacagg ggtaaccgtt
    18361 ttgccagtga cgaaattgtg tgcctgcaca cccggggcta agagcatgat aatcaacgca
    18421 ataatcaaac gagatgacat cattgctccg gctatagcgg aaaacgcgaa atcagcagct
    18481 ctaagcgctg acaaatatcg ctatccagcg ccaacggtgc ataatacgac tggcggtttt
    18541 ccatatcatc cgccaccatg tcgtcgtgtt tatctcccat caggccatta atggcggcga
    18601 ggaaacggga aatctgtaac gcctctttat tttcaggatc aagcgatctt aaggtgtcgt
    18661 aataagaaac ctgctggctg atgatttttc gcacatcatg aaattccctg ccggttagtc
    18721 gcggtttggc aataatctct ttcagggtat gcatttgctt ttgccgatag gcaataaaac
    18781 tgcttacgct ttctaactgg ctatggcgta atgaatagtt caccaacgac cagaccggag
    18841 agcttaaata aatccgtaac aggttgccgt aatacgacac aatatttttc ttaccgttac
    18901 gaaagccgtc ctgaaaacgc cccagaaaaa cggtcgtttt gccgaataaa aatccggcct
    18961 gatgcttctt taaatacagc cgttgcgcaa aacgcaggtg cttgtagcgc gctcgcatgt
    19021 atttaaattg tttgcgctca tcagcagtca gctcatcgcc tttggcttgt ttgcgaacta
    19081 aacgcagtaa ttcctgacga tcaacgccat tgagccagaa ttgcaggcat aacgcgtaat
    19141 tatcatgaat gatttcggtg gtacaactta gtgcaatagt cggcggcaaa ctaacctgtg
    19201 catcaacgac atcatcttct tcaatcgcaa caaatagacg ctcgctcgcc tcaagcggga
    19261 aatattttct catcatcagg atttctctcc ggctgcgtta taaagcaagg aatggcgctg
    19321 aaaaaatgcc tttaaggttg gaattaacga gagcgccacg tccagacatt gcccaccgaa
    19381 gtaagcaaac gctgctccca gcacaccaaa cagatgacag aacatgaaca gcaatacgat
    19441 gtagccgatt gaggcgatgg tttgcgccac gagaaaagcg cgctgttttc ccgccatcaa
    19501 tagcaatgac tcctgcggga agcccagcat cgaaatcaca attgcgccca gcatcacctg
    19561 aatcaggtca tacgcttcga gatacttaac gccaaacacc agcgaaatga gcggcttgcc
    19621 gacgatcaac accgcgagcg ccaccagaat accgattccg cccgccagta aaccggactt
    19681 cacacctaac aaccacggtc tggtggtgcg cggatctaaa cgcatcacct ctgggtaaaa
    19741 acttttaccc agcaaacccg ctggcgttcc ggcggcgtcg aagaatgtca tggcgatttt
    19801 aaataacccg gcggcagcgg gtcctaacac gatccccacc aacactgtgc tgcacgagtt
    19861 acgcgccgac cagatggagt gggcaatgtt ggttgaccag acaaaactcc acgcgccttt
    19921 aatgtgtcgg gcagactcaa acagattcaa tttgaaggcg ttatggatat ttctacggcg
    19981 taattcgcgc gcggcaaacc accagtacat ggtgccgcca accagattcg acacgtacca
    20041 agcaataaca aaacccacaa aaccaaagtc aaaataccag gctgcgacgc tccccgctgc
    20101 gcgcagaaaa ggtttcgtcg cctgctgtac agcaatcaaa tcaaagcgat ctaccgcacg
    20161 cagaatgccg gtcggcgtgg aggaagccat tgaaggaatg agcgtgcaat acagcgccgc
    20221 cagccaaaag ctttggtcat ccagacctaa tgaatgggaa aggaatggca gtaaggcaat
    20281 gccgccgaca atcgccaccg cgccgctgac gatatccagc gagaaggaaa atgagacgac
    20341 attgcgaaat tgctgcggat tattgttggt caaggctggc gttccgtact gaaccaccag
    20401 ctgccatgtc tgaaacttaa taaaatcgct gatcgacttg gcgtacgatt gcacaatcac
    20461 cagtacacca aacatggcgg gcgtcatccc tttaccggca cacgagagcg ccaacagccc
    20521 cagcaaggcg ctctcgacat tactggagcc taaccaggcg ctattgcgaa taatggtgcg
    20581 aaacgcgcca tctgcaaacc aatgtttgat gttaaaaccc gccaattcag cctgacctta
    20641 tcgttgatag taaaaaatat cccgccagcc ttaagttaaa cttcggcggt cagaaacgat
    20701 ggcaaccaga gaaaccgcct tctgtacctg ttccagcact tcgctgtagg gcgctctgga
    20761 atcaatctca agaatttttg tgccgttata gccaatcgtc gacatgacgc cgattttgtc
    20821 ctgcagctcg gcatactcat ggtcaggctt gcgggagatg gcagtatcaa tatcaatccc
    20881 caaacgaatg attaattcag gacggtattg cgccattttt tggtataaac tccgttcgcg
    20941 ctgcgccaga aacatgctga ttttcccggt cgcacgttcg acgccgatcc ccggtccgtc
    21001 ataataaaag cccgaaattt cagcctgcgg gaagcgatcg ctgaccacca gaacgccact
    21061 ttgcgccagt cgctgaacct tgcgtagatt cgccattctt cgcagcgaga agcagtacat
    21121 aatcaccgcc gcccatagcg ccggagattt ggttttcatg ctttgggttt tcgaggattt
    21181 ggccgccaat cgccgttcca gccagacgcc aaccaacggc aatcgtttga ttttgtcgcc
    21241 gtcttcgccg gagagcagcc ccagatagcg gcgctcggtt tgccagtgtt gttgcagcga
    21301 tttcaccagg tcggtggtca gcgtggattt accggtgcca tcacacccga ccaccgcaat
    21361 cagccccgga atgtagttgg gctgcggcgc ggttgtacta ttaacagttt gtgtttgtag
    21421 tgcatccatt cgttaaatca tcccctatcc attttggtga tcaggaaagc gcggcgtgaa
    21481 ttgcgcgtag tgacgcttgc ataatttcat cggctggttg ccgaccatcc agttcaagga
    21541 tcttggcgcc attgaatgtc agttgcggcg taacggcgat tttttcctgt aacgctgcca
    21601 gttggtggtc aggtttacgc gcaaacgcgg tttgttcatc aatgccaaga cgaatcaaca
    21661 atacgggtaa ataagacgcc atccattggt acagcttcaa ctcgcgctgc cttaacattt
    21721 ttatccaacc gttaccgccc gtggtttttg ccaattgcgg gccatcaaag cgaaaacccg
    21781 gcacttcaac ctgcgggtag cggtcggtga tgagcagata gccttgctgg cttttacaca
    21841 acattttgcg aaacttgtac gcccgccagc aggaaagcag atagataacc agcgcagtaa
    21901 tattgccagg cggagttaag ggcttttcgt gtacatgtgc cgctttactt cgcagataac
    21961 acccacaagg tgcgccaata acagggagct gtgaaatcca ttcgccaatt cgcccggacg
    22021 attgcccgag ataaatgtgt tctgttggca ttcttgctgc cagttcattt accaggcttg
    22081 ccgtgagggt cgatttacct gaaccatcac atcctacaat ggcaataaca cgcactggcg
    22141 tggaattaat tattgacata ttacgttgat tcacgaaaaa cccggcagta aataatgtat
    22201 tgaatattac gtggtccgtg ctcagaatat ccgttcaacc ttattgtgac aatgactaat
    22261 tgtgacattg atctgaatta cgctttcata aaaacatatt aaccaaataa atatttttaa
    22321 tggatattta aattaaagga tatattcatg cagtcaataa cacctccatt aattgccgtt
    22381 attggtagcg atgcttcagg caagtcaacg gtgtgtgaac atcttattac cgttgtcgaa
    22441 aaatatggtg ctgccgaaag agttcattta ggaaaacagg ccggaaatgt cggtcgtgca
    22501 gtgacaaaat tacctttgat gggaaaatcc ttacataaaa caattgaacg aaatcaggtg
    22561 aaaacagcaa aaaaattgcc tggaccagtt ccggcgctgg taattacagc gtttgtcgcc
    22621 cgtcgcttac tgcgctttcg tcatatgctt gcctgtcgtc gtcgcgggtt aattgttcta
    22681 accgaccgtt atcctcagga ccaaattcct ggcgcttacg atggtacggt gttcccacct
    22741 aacgttgaag gtggtcgttt tgtctcatgg ctggcaagcc aggaacgtaa agcgtttcac
    22801 tggatggcga gccataagcc tgatctggtc atcaaactca atgttgacct tgaagttgcc
    22861 tgtgcacgta aacccgacca taaacgggaa tcgctggcga ggaagattgc cataacgcca
    22921 cagttaacct ttggtggtgc acaactggtt gatatcgatg ccaatcagcc actggaacag
    22981 gtgttggttg atgcagaaaa agcgattacg gattttatga ccgcgcgtgg ttatcactag
    23041 tcaaaaatgg aaatgcccga tcgccaggac cgggcatttt caggaaggtt aaatcaactg
    23101 caatgctatc cagtacagcc caccagaaag aaaaattgcc gccggtaaag taaacaccca
    23161 cgccatcagg atgctggtta ccgttttacg ctgtaaccca ccgccgtcca ccaccatcgt
    23221 ccctgctacc gcagacgaga ggacgtgtgt tgtggagaca ggcatcccaa tataactggc
    23281 aaggccgata gacactgccg ccgtcatttg tgccgccatg ccttgcgcat acgtcatgcc
    23341 gcgcttacca atcttctcac cgatggtcat cgctacacga cgccagccaa tcatggtgcc
    23401 aatgccgagc gccagtgcta ccgccatgat gatccacacc ggagcgtact caatggtgct
    23461 taacatatcg ctgcgcagtt ttttcagcag gttctggtct tctttactga cgcctggcag
    23521 tttcgccagc ttcgcggagg tatcggagat gcacagcata atgcggcgca gctggctgcg
    23581 ctgactcacg cttaacggct cgtaactttc catattgcct ggcagcatcg ttttaacgcg
    23641 cgcaatcgca tcaaaggtat ttgccggatg acagtgaaac tctgttactt gcgtgccatc
    23701 agtcgatgct gcaggcaatg gaggttccat cgcaatcaac ttctgcggca gttcaggatg
    23761 ctgttgcagg tagtgttcga agttggtaac ggcatcgcgg gtacgggtaa tttcatagcc
    23821 ggacgcattc atattgacga cgaagccagc aggggcaatc cccaccagta ccagcattac
    23881 caggccgatc cctttctgcc cgtcgttcgc gccgtgcgaa aacgccacgc ccgcagcgga
    23941 aacaatcagc gcaatacgcg tccagaatgg cggtttacgt ttgccctttt tctttttgcg
    24001 atcttccgga atgcggtgaa tacggtcacg ctttttcgtc ccactccaga aacggcgcag
    24061 caggaaaatc agaccacccg caatgaccag gccgacgata ggggaaacaa tcagcgagga
    24121 gaaaattttg gttacttcac gcaggtttaa cgcatccatc accgatgagc cggttaacag
    24181 cgcgttggtt aaaccgatgc cgataatcgc accaatcaag gtgtgcgaac tggaggccgg
    24241 taaaccaaag aaccacgttc ccaggttcca gataatcgcc gccagcagca tggaaaagac
    24301 catcgccaaa ccgtgagttg accccatatt cagcaacaaa tcgattggta acatatggac
    24361 aatggcatag gcaacgctaa gtccgcccaa taacacgcca aaaaagttaa aaaatgccgc
    24421 catcaccaca gcaagttgtg gctgcatggc acgagtataa ataacggttg ccaccgcatt
    24481 cgccgtgtca tgaaaaccat tgattgcttc gtagaacaac acaaatgcca gagcaagtaa
    24541 taacaaaagc cccgtatata tatcaaggcc aacaaataaa tttagcatat attagattac
    24601 gccattttga atttacgaac ggacgcatta tcagtgactt taacggcatg ggcaaagtga
    24661 aatatcattt tttaacgata aaaagagtgt tatttcttgt gtatcagaca ataagcattc
    24721 attcaaaacc tttagaaata atcaaaacac aggtattcca ctggttaata tattcacata
    24781 tgaaatgaat aaatattgga atatataaat attgaatatt ttgattaatc cccgtactaa
    24841 ttattcttca taatcagtac ggggttgcta caacatgtat tactttttca ccacaattaa
    24901 cggttcaata tcactctctt ttttgatgac cagtgattca tcaccgcgca aacacgtccc
    24961 accgtagttg ccgccaacgg tgaaggtaca tacctgaatg tatttaccgt ccactttcgg
    25021 caaacaccac agttgctgat agatgttttt ctgctcggca aatttaccgc tggttttgtc
    25081 cagcacctct tcatgatggc tgaccaggtc gatattgcta ccacagcgac cggcgatcgg
    25141 tttcactgcg taacctgttt tcaccaattc atcattaaca gtgaaatcgg tatccagcag
    25201 gtaacgatgg tgcgggaaca gcgaccagag gatcggcaga atcgctttgt tgccggggat
    25261 caccgtccac agcggctcaa agaccagcac ttccgggcgc agcaatacgt cgataagacg
    25321 cacttcgttt tgcggatgac cggtacggat tggcaccgca gcaaactcac ggtcgctaac
    25381 ttcacgaatc tgatcaaacg cggtttccca cgcccaggtt ttccacacgc agttaaccag
    25441 tcgcccttcc ccatcaatca gttgcccggc agcatcccag cccagttcat ccagcccgcg
    25501 caagatacgc gtttcaaagc ccgcctggtg cagcgcctgc tccataaact gcgcgtgata
    25561 gttttcctcg atatctttgt cctgcatgat atggacaaac ggacgtgcac gactgtgttt
    25621 ccaggcacca gccagttcgt taatcagccc ttccgccgga ttgaagccgt tgcctttata
    25681 gccctgctcc gcccaacgtt cgaggatcaa gcccgcttcg gtatgacagg aggcggagtc
    25741 ggcgttgtat tcgtaaacct tcaggccacg ctcatccatg cagaaatcca tacgaccagt
    25801 gatcatatgg tgacggcgac gctgccagga gagacgcaaa cgtggccaga ggattttcgg
    25861 aatgtcgaac agcgccagca ggttgtcatc tttcagcacc ttgtcggttg cgtgaagata
    25921 catcagatgc agctcgttgg tggctttaat tagctcctgc tcggcactct cggtaatggt
    25981 gtagtaatga tacggatcct gattgatcac ctgaccgttg gcctgcacat aggcgttttg
    26041 tagcggatct ttttcatcca gccatttacc gtcaaactgg cctttgtttt ccagacgcgc
    26101 tccactgatt ttcagcagct cgcctgcaat ttccggctgc ggtaagctgt attcagtatc
    26161 ttccgtctgg atcatccagc ccagaatggt ggtgtcatca aaagtgtctt taagggtata
    26221 gcagccgttt tcgaccacca tctccagctc gcgcgtccac tgttgccctt gcggcaacgg
    26281 ggaatgaatc acgttctgtt ccgcaatacg gactttgttg ccatgcaatt gggtaatgat
    26341 ggcgacatgg ccagtgtctt taaattcacc gcctttatcc cagataagaa gcgcacccgc
    26401 gaccggcgca cgcggcgagc cgttaggaaa tgcctgcaat ggcaggatgt tgtcattaac
    26461 cacttctcgc aggaagcgca gcgagaaaat ctcccacgcc atacccacgt cagtaaagac
    26521 cacaccgtaa ttcagaaaga gaaaacggcg agcaaattca acgcattgcc acttgtggcc
    26581 catatattcg tcgtcgatat agctacggaa tacggcgtca tcttcgtatt cctgcggatc
    26641 gagagaactg taatctgaag agtagattgc taccccacct ggggcgtagc ccaataatgt
    26701 cccgaacggg gcatcctggc tggtcgttcc tttgctcatc actttacctt taacaataca
    26761 acctaagcag ttggcgtaat tgttgtgctc tgattacctg ctcagcgaca ttaaccggac
    26821 agaggtcaga ctaatcatac acctcatcgc agcggctggc gcaaagggtt aaaaaattca
    26881 cattctgtac agcaagtgac ctgctacact gcttcaacac taccactcag aaggcaactc
    26941 actatgacag acaatactta tcagcccgcg aaagtctgga cgtgggataa atccgctggc
    27001 ggcgcgttcg ccaatatcaa tcgcccgttt tctggtccga cgcatgaaaa aacgctgccc
    27061 gttggcaaac atccattgca actttattcg ctgggaacgc cgaacggtca gaaagtaacg
    27121 attatgcttg aggagctact ggcgctgggc gttactggtg cagagtacga cgcctggctg
    27181 attcgtattg gcgatggcga tcaattctcc agcggctttg tcgaagtgaa cccaaactcg
    27241 aagatcccgg cgctgcgcga tcatacgcat aatccgccga tccgcgtgtt tgaatctggt
    27301 tcgatcctgc tttatctggc ggagaaattt ggctacttcc tgccgcagga tctggcaaaa
    27361 cggactgaaa cgttgaactg gctgttctgg ttacagggtg ccgcgccgtt cctcggcggt
    27421 ggttttggtc acttttacca ttacgcgccg gtgaaaattg agtacgccat caaccgcttt
    27481 accatggaag ccaagcgcct gctcgacgtg ctggataagc aactggcgca gcataagttt
    27541 gttgcgggcg atgagtacac cattgcggat atggcgattt ggccgtggtt tggcaacgtg
    27601 gtgctcggcg gcgtgtatga tgccgcagag tttctggatg cgggaagtta taaacatgtg
    27661 caacgctggg cgaaagaagt gggcgaacgt ccggcagtga aacgtgggcg tattgttaac
    27721 cgcaccaacg ggccgctgaa cgagcagttg catgagcgcc atgacgccag tgattttgag
    27781 acaaatacag aagataagcg tcaggggtaa gggttggtgt tcgtcgcagc aagccatcca
    27841 ggccggataa ggcgttcacg ccgcatccgg caatcgtgca caatgcctga tgcgacgctg
    27901 ccgcgtctta tcaggcctac aaaagctaat catcacgcgc tggtaatgtc gtaatccatt
    27961 tggcgcagtg cgtctaatgt ggctttggct tcatcttcgt cgatgatgct catggcaaat
    28021 ccgacgtgta ccagcaccca ctggcccagc agatcggcag ggttaccttc gcaaatcagg
    28081 gcgatattca cgtcgcgctt gataccacat acttcaacct gcgcaagctg gtgaatatct
    28141 tcaccgacag ccagcacctg gcctggaacg ccaatacaca ttgttaactc cggttatttc
    28201 gattgccgga caaacgcgaa aaagaacctt tagcgggtgt tccctctcac cctaaccctc
    28261 tccccggagg ggcgagggga ccgtccgtgc gctttttagg cgttgtcctc agtcttattc
    28321 aacttcaata cttttgacga tcagcgaatc gccggtatcg acacgcaacc gctcgccgtg
    28381 acagagcgga cactgcgcat cgtgctgatg aatctccacc acctggctgc aatcccagca
    28441 ccaggcctgg gcgggtttat agacgatatg taaatcgcac ccttgcgcca ccgttccgtg
    28501 gcagacaatt tcaaaactaa aacggacggc gctctcctca acgcaggaga gcgcgccaat
    28561 ttccagccac acggcggtga cgcgcttaac atcgtgctgc tccgcctgcc gttggataat
    28621 ttcaacggcg ctctggcaaa gcgacaactc atgcattttc gcccctcctg cggccaaaca
    28681 gcagggcgcg acgccctgcg tgtggtacat ccggattggt gactggcaac gaaaggatca
    28741 ttcgtgcgca gtcatccgtc aggcgttggc cttcttcaat cgacatgctg tgcgaaagcg
    28801 gcgacatcag cgaacaggag aggtattgcg aaacaccgtc cagttcgcca acggtaaagg
    28861 tcatggtgcc atacggcagt tgcagaccaa ttttttcact cactttgcgc agcggccaga
    28921 gttgatccgg gccggggaag ataactgcac tcagcatcca cggggtgatc acacaccccg
    28981 tccactgacc ttcgaacagc gtaaaatcag aaacatacac cggcattgaa ggatgcagaa
    29041 aagagagatc gtgcatcgaa cgccgggcaa tttcttcaaa cgctgcctgt acttgcgcct
    29101 tcggggaggt ctggaaacct gctatctctt cagtcatgaa tcgcctcccg tgggatggct
    29161 tccacgcctg attcgcgcag cgcagccaga acctgctcaa gcgcaggttc aatcattgct
    29221 tcaaccgtcg gcgttaagcc gatgtgtggc tccagcgatt ccgggatcac gccgaccagg
    29281 gtcagctttt tcggaaactc accggtgaag cgcagggccg acaatacgtc ggccaggcca
    29341 agctgatgcg gagagatttt gttggtaaac aacgccggaa cttcttcatc ccgcaggatc
    29401 atcatcgttc ccggagtgtt ctttttcgac acaatggcat ccgcaataat cagatgatcg
    29461 cgatttgcca tgtcgccaag cagctccatt cccgccgtgc cgccatcgag gatctcaaca
    29521 taatccggca gaatgtatcg ttgctctaac gcttcgacaa tccgcacgcc gatggcttca
    29581 tcggtcagca aaatattgcc gacccctaag actaaaatac gcattacaga accttcactg
    29641 aaaccacttc gttgccgtcg gcatccacta cgtgtaccgc acaggccatg cacgggtcga
    29701 aggagtgaat ggtacgcacc acttccagcg gtttattcgg atcggcaacc ggtgtaccca
    29761 ccagcgactg ctcgtaagga ccgacgtcat cattgaagtt acgcggacca gagttccagg
    29821 ttgatggaac aaccgcctgg tagttgctga tgataccgtc tttaataacc atccagtgag
    29881 agagcatacc gcgcggcgct tcgaggaagc caacaccttt gaactcaccc gttgccggaa
    29941 tgttcggctt cacaaaggtg gtgtgatcgc ctttgccgat attggtgatc agtgcactgt
    30001 attggttttg caggatatcc tgcaattcac agcagtgaac ggtacgacca ataatacggc
    30061 ccagcgtgga gtgcagctgt gccacttcca gcgtgttgcc agtcagtttc tgataaatcg
    30121 caacgatttc attcagtttg ttctgggtag attcgcgacc tgccgccagt ttcaccagca
    30181 tattagccag cggacccact tctaccgttt tgccgtagaa agtcggtgat ttcacccagg
    30241 aatatttccc gtcgtcagac caaccatcat aagccggaat ggtggtacct tcccacggcg
    30301 cctgtggcgc ttcgtcttta taccaggagt gcttcgcgct ttcctgaatg cctttgatca
    30361 ggtattcatc ggaatgagaa gtgatcggac gatacgagga cagatccgca ttctcaatgt
    30421 agcctcccgg gaacaggaag ctgccgtttt tgctgtcggt cgggaattcc ggcacgctca
    30481 ggtagttcac cgcaccttta ccgcgcgtca gccattccgg gtagaacgcg gcgataaccg
    30541 cggtatcaac cttataaacc tgctcaacaa agtcgctcag tttgtcgatg aaagacttga
    30601 tgtacatcag gcgctcaagg ttcaacacgc ccaacccgtc gaggttgatt gggttcgcga
    30661 caccacccac agccaggttc tgaatgtgcg gcgttttacc gcccagcagc gccacgacgc
    30721 ggttagcgtc acgctggcac tccagcgcct gcaggtagtg cgctaccgca atcaggttca
    30781 cttccggcgg cagtttcatc gccgggtgac cccagtagcc attggcgaaa atacccaact
    30841 gaccgctggc aaccagatct ttgatcttgt tctgaacttt agtgaactct tccggactgt
    30901 tcagatgcca ggtcgaaacg cctttcagca tttcggaggc tttggttggg tcagcttgca
    30961 gtgcagaagt gatgtccacc cagtccagcg ccgaaagctg atagaaatga acgatatggt
    31021 catgcgtggt gtgcgcagcc agaatgatgt tacggatgta ttgcgcgtta accggaacgt
    31081 cgatattcag cgcactttct gccgcacgaa cggaagacag cgcgtgagtg gtagtacata
    31141 cgccacagat acgttgcaca atcatccatg catcgcgcgg atcgcggttt ttcacgatct
    31201 cttccatgcc gcgccacatg gtaccggaag cccatgcttt cgaaacgacg ccattttcga
    31261 tttcgcaatc gatgcgtaaa tgcccctcaa tacgggttac cggatcaata gtaattctct
    31321 ggctcatgct ttgctcgcct catgacgatt atgatcgttt tgttttaaag gaggaagtat
    31381 cggcagtaga cgaatgagta cgatgtaagc gcaaatctca atagccacaa aaccaataga
    31441 aatcaacagt tcttcccagg tcgggaagta ggcgtaaccg ccgcccgggt tgaatgccac
    31501 caacgaatag gtcagacgcc aggttgcaca acctaacagt gcgctcagtg ctgacaggaa
    31561 cagcatacgg gaatcattac gcagcttcgc cacacgcaga acgaccagcg ggaagagcat
    31621 cagcaggact tcaatccaga acatcacgga gtagaagtca ccggcaaacg ctaacgacag
    31681 cttgtcgcga tagataagct cgccaaagcg cagcccgatg aaaatcgcca gcaacacact
    31741 gatggtgttg gtcagcttga cgaacaggct cttttcatcc ggaccgttgc cacgcagacc
    31801 cgcctgcacc agtgaacctt caaagatgac aatcgagaag cccatgatga acgccgtcag
    31861 cagcgagaac agcggcaaca tttcatagct ctgccagagc ggatgcacct tgtagcccgc
    31921 cgagatcatc agcgacccca ttgaagactg gtgcatggtc ggcagcagcg caccgagcgc
    31981 gatgatgaag aacatcacct tgttcagacg ttgtagcgac accttccagc ccagacgttc
    32041 aaacagtgcc ggagcaaact ccagagccat cacgccgatg tagatggtca tacagaccgc
    32101 cgtctcgaac agtaccgagt tcacgttgaa gtgacccgga atgtagaagt acggcaggtt
    32161 ccagtagcga cccacatcga tagtgatcga caagccaccc agtgagtaac caaacagact
    32221 cgccaacagc gccggacgca ccagcggatg gtattgccca cggttaaaga cgtataccgc
    32281 ccacgccagc gcccagccgc cacaggcaaa gccggtgcca atcagcaggt caaacgcgat
    32341 ccacacgccc cacgggaagc cgccgttcag gtcagagaca gagcccagac cgaacaccag
    32401 acgcttcaca atcaggagca tacagatgac gattaacggt ccaaaaatca tgaccggttt
    32461 actgatgatt ttgccgccca gcggttgtgg atcatgactc atgatcgtct cctccgtcgt
    32521 gatggtcgtt tttggtgttg cgacgaacca gcacggttaa gcccgccagc acagccagtg
    32581 gtagcatcat gcctttatac agggtgtgtt gaatattttc ggaacgcgca ccggtagaaa
    32641 gatcgtccag tttcggcagg tcgagatttt cataaggcac acccgtcagt accagaacct
    32701 gagtaccgcc gccctctttc tcgccgtaca gatgcggata atatttcggc accgtatgca
    32761 ggtaagtgtc gccagatttc agcgtctgac gcggatagtg gtattcgctg ccaggcttca
    32821 gcgccagacg ttttttcgcc tccgccatca gctcttcacg cgtaccgaaa atcaccgcgc
    32881 ccgccgggca cacttctacg cagccaggca gaccgccttt atcgagacgt tccacacctt
    32941 tctggttgca cagctcgcac ttatgcagcg caccaaacgg gttgttgtag tcgtacttcg
    33001 gcacgttgta cggacaggcg accatgcagt aacggcaacc ggtgcacacg tctttgtcgt
    33061 aatggacaat gccggttttc ggatcttttt tcagtgcaga gaccgggcac acagagacac
    33121 agttcggatc gacgcagtgc atacactgtt tcttaatgta cgcgtagccg ttctcctcct
    33181 ggtctttgtt gacccctgtg ccgctggtcc acacctgaat gatgttattg gtatacggcg
    33241 acagtttgtc gttgttcgac caggtctgtt ccccttgcgg gttacgttca gggaagttga
    33301 tatcctgaca cttggtgacg caagcctggc agcctacgca caaggtcgag tcgtacaaca
    33361 tccccagcga tcccggaatt ggcgggcggt tttcagcagc cgcatgactg acagacggca
    33421 gcgcgcccgt cagcaatgcc ccgcaggagg ctgctttaat aaaattacgt ctgttcacgg
    33481 ttattctccc cgtgagtcag cgttatcttt cttttgctga cgacccagtt cacgcaccgc
    33541 catcacgctg acaccggcaa ccagcccaac cacaccgccg agcaaaccaa tagcgcctgc
    33601 agagacgttg ccgccctctt tagcgttaac atccggtttc tgtgaacgcg gagtctgatt
    33661 ttcgacgttg gcaagctgat ggatgccttt atggaagccg ataccttttt cgttacagcc
    33721 atagcacggg tgaccaatcg ccaccggcca cacaccgcca acatcgcaga attgcagcgt
    33781 tgagcagttg ccgtaagttt ctggcccttt acagccgagg tggtacaagc accagccttc
    33841 gcggtggcct tcatcaccga actctttggc aaaacgacca gcatcgaagt gcgggcgacg
    33901 ttcgcagtgt tcgtgaatca gacggccata ggcgaaggtc ggacggtttt tgtcatccag
    33961 tttcggcggt ttgccgtaag tgatgatgtg cgcaacggtc gcgaggaagt tgtgcgggtt
    34021 cggcgggcag cccggaatat tgataacggt tttgcctggc agaacttctt gcaagctgac
    34081 tgcgccagtt gggttaactc cagctgcggc aacaccgccc cacgcggagc aggaaccaat
    34141 agcgataatg gctgctgagc cttccgccgc tttgcggatg tgatccacaa tcggctcacc
    34201 ggcaaccatg caataaatac cgttatcttt taatgggatg gaaccatcca ccactaacac
    34261 atactgccct ttgtacttct cgagagcgtt atgtttgttc tcttcgacct gatgaccgaa
    34321 ggcggcggaa agcacttcgt gatattccag agagatagtc tccagcacga ggttttctac
    34381 cgttggatgc gttgcacgaa gcagagattc cgtacaaccg gtgcactcct gcgcgccaat
    34441 ccagataact ggcggacgct gcgggttagt aaccgattcg gccatctctg cagcggcttt
    34501 gctacttaac cccatggtgg cggctaatgc tgcacaaagc ttcatgaaat cacgacggtt
    34561 aatgccgtga gaatggatga gggtgttatc tccagtcatt tatagttatt ccgttgcgag
    34621 aacctggcat atattttgcc tcaatcgcaa aatcaataat gcgatcgatg cgccatttac
    34681 cacacattta ttatggttat cgatatcatg atactgcggc ggggcgctaa aacgaaggga
    34741 agaagcatta gtgtaattaa ttaaagcaag ataatacgta tgtttgatca attttcgagg
    34801 cgataaccgg ccataaaaaa gcgcaacgta gaacaggaat tattctcacg aaagaaatcc
    34861 attaccatct ctttatccag tccatagcgg cgcgtcagaa tacccgcttc ccaggcgcta
    34921 agctggcgat ctccggtctt ttcgtacatc ctgtgtgagt agcctaatac aaaaccgcgt
    34981 ttataatcgg cacagaattt tgtcacatta accgcactgt cggcatgcgt tgcttttaat
    35041 cccgccatta agcctttacc aaaatggtta ttcatcgttc acctcattca ttatatatcg
    35101 ccatatataa cgatatttta ggcaaagaac gacttttgcg cacgctattt tttcatactt
    35161 gtttaagacg taatcccgta agagataaac ctatcaggag agatatttat cgaaccattt
    35221 cagtgtcctt tgccaggcaa gatcggcggc agatttgtca taacggggcg tggaatcatt
    35281 atggaatccg tgattaaccc ccggatagat ataagcctca taaaccttat tattgtcttt
    35341 caacgccgcc tcgtaaggag gccagccctc gttgattcgg gtgtccagtt ccgcgaagtg
    35401 gagtagtaaa ggcgcttcaa tcttcgccac atcggcagtg ggtgcctgac gaccataaaa
    35461 cggcaccgcg caggccagtt ccggatacgc gacagccgcc gcgttcgata cgccaccgcc
    35521 atagcaaaat ccggtaatac ccactttacc tgtcgcttgc ggatagcgtt gcataaactc
    35581 aattgcggca aagaaatcat tcatcagttt ggttggatca acctgctgtt gcagctcacg
    35641 acctttatca tcatttcccg gataacctcc aacggaactt aatccgtcag gtgccagggc
    35701 gatatacccc gccttcgcca ctcgccgtgc cacatcttcg atatacggat tcagtccacg
    35761 attctcatgc accaccccca cggctggcgt tttgccgctc atttttgcgg gtttcaccag
    35821 ataaccccgt acctcgccgt gaccatttgg cgaaggatac gtgatgtact cagcaacaat
    35881 ctccgggtcg gtaaactcta cctgagtcgc cagcgcataa tttggcttga gcaaatcaaa
    35941 caacgctaat gccgtcatcc cgcccaccgc atacttcgcc gcaaggttga ggaactcacg
    36001 tttcgaaatt ttcccgtgaa cgtaatagtc gtagtaatcc aacaactctt gtgggaaatc
    36061 tttggcggtt aaacgcggca tcgttgcatt cctcaactgg tgttttttta agcaaagcat
    36121 aagcgcgtgt ttttgcccag tttttcgtca ctctgtgagc cagactacgg gatacgcgct
    36181 ggcgaatcgc taaactagaa acattgtttc gaaattgaac ggtggaaagg agaggtcatg
    36241 gtctggttag cgaatcccga acgttacggg cagatgcagt accgctattg cggaaaaagt
    36301 ggtttacgcc tgcccgcatt atcgctcggt ttatggcaca atttcggtca cgttaacgcg
    36361 ctggaatcac agcgtgcaat cctgcgtaaa gcgtttgatt taggcattac gcactttgat
    36421 ttagccaaca attacgggcc gcctccagga agcgcagaag agaactttgg tcgcctgctg
    36481 cgcgaggatt ttgctgctta tcgcgatgaa ctgattatct ctaccaaggc tggctacgat
    36541 atgtggcccg gcccgtacgg ctctggcggt tcacgtaaat acctgctcgc cagcctcgac
    36601 caaagcctga agcgtatggg gcttgagtat gtcgatatct tttactctca tcgcgtcgat
    36661 gaaaatacgc cgatggaaga aaccgcctct gcgctggctc atgcggtaca aagcggtaag
    36721 gcgctgtatg tcgggatctc ctcttactcg ccagagcgga cgcaaaaaat ggtcgagttg
    36781 ctgcacgagt ggaaaattcc gcttttaatt catcaacctt cgtacaattt actgaaccgc
    36841 tgggtggata aaagcggcct gctggatacc ctgcaaaata acggcgtggg ctgtattgcc
    36901 tttactcctc tggctcaggg attgctgacc ggaaaatatc tcaacggcat tccgcaagat
    36961 tcacggatgc atcgtgaagg gaataaagtt cgtggtctga cgccgaaaat gcttaccgaa
    37021 gccaacctca acagcctgcg cttattgaat gaaatggcac agcagcgtgg acaatcaatg
    37081 gcgcaaatgg cgttaagctg gttgctgaaa gatgatcgcg tgacgtcggt attgattggt
    37141 gccagccgcg cggagcaact agaggagaac gtgcaggcgc tgaataatct gacatttagc
    37201 accgaggagc tggcgcagat tgatcagcat atcgccgatg gcgagctgaa tctgtggcag
    37261 gcgtcgtccg ataaatgacc tgttaataac gggcgcgcga accgcgcccg aatcagatca
    37321 gtgattatga cgagtcagtc ggtcaagata gcccatcaca aatgcagaaa gcacaaacgt
    37381 cagatggata atgacgtacc acatcagttt gttatccggg acatttttcg catccataaa
    37441 gacgcgcagt aagtgaatgg aagaaattgc cacaatcgac gctgctactt tgtttttcag
    37501 cgacgttgcg tccattttcc ccagccactt cagcttctct ttgttctcgg agatatccag
    37561 ctgtgagacg aaattctcat aaccggaaaa catcaccatc accagcaaac cgccaaccag
    37621 cgtcatatcc accagcgaca gcaacacgag gatcaaatct gattccgcca tcgagaagat
    37681 attcggcagt acgtgaataa tctcctggaa gaacttcagc gccagggcaa ctaacgccag
    37741 cgaaaggcca aagtacacgg gggcaagcag ccagcgagaa gcatacattg cattttcaag
    37801 aaaacgttcc atacattcct gtcgtgtagt taaaacgggc aacagtatat cgcaattgag
    37861 taaacagcac gcaacagcaa atcaacatat acgttaaacc tttgtcgcat aattctgagg
    37921 tttagggatc ttcctggttc ccttatcaac gcatcgagag cgtctaccct taaaggacgt
    37981 tgaacctgaa gggagaaaaa cgatgtctca tttaaaagac ccgaccacgc agtattacac
    38041 tggtgaatat cccaaacaga aacaaccgac gccaggcatc caggcgaaga tgacaccgat
    38101 accggattgc ggcgagaaaa cctatgttgg tagcggtcgc ctgaaagatc gtaaagcact
    38161 ggtgacaggg ggcgattccg gaataggtcg cgctgccgcc atcgcttacg cgcgtgaagg
    38221 ggctgacgtg gcgatcagtt atcttcccgt ggaagaagaa gacgctcagg atgtgaaaaa
    38281 gatcattgaa gaatgcggac gcaaagccgt tctgctgcca ggcgatttaa gcgatgagaa
    38341 attcgcccgt tcgctggttc acgaagcgca caaggcgtta ggcgggctgg atattatggc
    38401 gctggtcgcc gggaaacagg ttgccattcc ggatattgca gacctcacca gcgaacagtt
    38461 tcaaaagacc tttgccatta acgttttcgc gctgttctgg ctaacccagg aagcgatccc
    38521 cctgctaccg aaaggtgcaa gtgtcatcac cacttcgtca atccaggcat accagccaag
    38581 tccgcattta ctggactatg cggctacgaa ggcggcgatt ctgaactaca gccgtggcct
    38641 ggcaaaacag gtcgcggaga aaggtattcg ggtgaatatt gtcgcgccag gccctatctg
    38701 gacggcactg caaatttccg gcggacaaac gcaggataag atcccgcagt ttggtcagaa
    38761 aacgccgatg aagcgtgcgg ggcaaccggc ggaactggcc cctgtatatg tttatctggc
    38821 aagtcaggag tcgagctacg tcaccgcaga agtgcacggc gtgtgcggcg gcgagcattt
    38881 aggttaaaaa aatgcccggt tgtgaaaagc aaccgggcat cattgtgaat tcactcttac
    38941 ctgatgtggc gaaaacgcct tatccgccta caaacgcatg aagattcaac atgttgcaat
    39001 agattttgta ggcctgataa gcgtagcgca tcaggcattg agcacctaat gccggatgcg
    39061 tcgtgaacgc cttatccggc ctacaaacgc acgaagattc aacatgttgc aatagatttt
    39121 gtaggcctga tgagcgtagc gcatcaggca attctacttt acttcgcttt ggcggtttct
    39181 tcgccgacca gacctatctt caggtaaccc gcctgatgca gcgtatccat taccttcatc
    39241 aacgtctcgt aatcgacggt tttgtccgct cggaagaaga tggtggtgtc tttcttgcct
    39301 tcggttaacg cattcaacgc cgtaatcatt gtttcatcgg tgaccggatc gttaccgata
    39361 aacatcgagt tgtctgcctt caccgacaga taaaccggtt tttccgggcg cggctgcggc
    39421 gtgctggtag aagcaggcaa gttcaccttc acatctaccg tcgctaacgg tgccgccacc
    39481 ataaagataa tcagcagaac caacatcacg tcgataaacg gcgtcacgtt gatatcatgc
    39541 atttcgccgt tatcgtcgag gttttcgtta agatgcattg ccatcggaca ttatcctgcg
    39601 cgtaattttt gtgcgacacg aaccggatgc gcagcggcgc tggcttccag atctaggtca
    39661 cggctttgca gcaacaatac ctgcgctgca acatcaccca gcatcgcttt aaagccgcca
    39721 atctggcgtg caaatacgtt atagataacg accgcaggaa tcgctgccac gaggccgatt
    39781 gccgttgcta acagagcttc tgcgataccc ggcgcaacga ctgccaggtt agtggtctgc
    39841 gtttgcgcga taccaataaa gctgttcatg atgccccaga ccgtaccaaa cagaccaacg
    39901 aacggagaaa tcgcgccgat ggttgccaga tagccgttac cgcggcccat ttgacgaccc
    39961 actgcggcga cccgacgctc cagacggaag ctggtacgtt ctttaatacc ttcgttatcg
    40021 tcgctgcctt ctgacagttc cagctcattc tgcgcttcat tgagcaaatg caggcttaag
    40081 cttttgctac caaaatcagc ggcgatatcg ttggcctggt ttaaggaacg tgcttcagcc
    40141 agcagttgct gctcgcgctt aaggcgacgc ttctgattga agaactctac gctcttactg
    40201 aagaagattg cccaggtgac tacagaggcc aaaataagcc caatcatcac gcacttaacg
    40261 acaatatcgg cgtactgata cataccccag acggaaaggt ccgtctgcat taaattatta
    40321 cccactgtgt atctccagga cgcaagtcac aaaatctgcg cataataata tcaaaacgac
    40381 gtcgaattga tagtcgttct cattactatt tgcatactgc cgtacctttg ctttcttttc
    40441 cttgcgttta cgcagtaaaa aagtcaccag cacgccattt gcgaaaattt tctgctttat
    40501 gccaattctt caggatgcgc ccgcgaatat tcatgctagt ttagacatcc agacgtataa
    40561 aaacaggaat cccgacatgg cggacaaaaa gcttgatact caactggtga atgcaggacg
    40621 cagcaaaaaa tacactcttg gcgcggtaaa tagcgtgatt cagcgcgctt cttcgctggt
    40681 atttgagagt atggaagcca aaaagcacgc gacgcgcaat cgcgccaatg gcgagttgtt
    40741 ctatggacgg cgcggaacgt taacccattt ttccttacaa caagcgatgt gtgaactgga
    40801 aggtggcgca ggctgcgcgc tatttccctg cggggcggca gcggttgcta attcgattct
    40861 tgcttttgtc gaacagggcg atcatgtgtt gatgaccaac accgcctatg aaccgagtca
    40921 ggatttctgt agcaaaatcc tcagcaaact gggcgtaacg acatcgtggt ttgatccgct
    40981 gattggtgcc gatatcgtta agcatctgca gccaaacact aaaatcgtgt ttctggaatc
    41041 gccgggttcc atcactatgg aagtccacga cgttccggcg attgttgccg ccgtgcgcag
    41101 tgtggtgccg gatgccatca ttatgatcga caacacctgg gcagccggtg tgctgtttaa
    41161 ggcgctggat tttggcatcg atgtttctat tcaggccgcc accaaatatc tggttgggca
    41221 ttcagatgcg atgattggca ctgccgtgtg caatgcccgt tgctgggagc agctacggga
    41281 aaatgcctat ctgatgggcc agatggtcga tgccgatacc gcctatataa ccagccgtgg
    41341 cctgcgcaca ttaggtgtgc gtttgcgtca acatcatgaa agcagtctga aagtggccga
    41401 atggctggca gaacatccgc aagttgcgcg agttaaccac cctgccctgc ctggcagtaa
    41461 aggacacgaa ttctggaaac gagactttac aggcagcagc gggctatttt cctttgtgct
    41521 taagaaaaaa ctcaatgatg aagagctggc gaactatctg gataacttca gtttattcag
    41581 catggcctac tcgtggggcg ggtatgaatc gttgatcctg gcaaatcaac cagaacatat
    41641 cgccgccatt cgcccacaag gcgagatcga ttttagcggg accttgattc gcctgcatat
    41701 tggtctggaa gatgtcgacg atctgattgc cgatctggac gccggttttg cacggattgt
    41761 ataacattgc cacttttgga caattttgca gacattttat tgtgaaaagt cttaaattgt
    41821 tgcgtccggg atcaaggcgt cccggacgat tcaggagtac aataggcaga taaaggctta
    41881 aacgctgttc cacaggaaag tccatggctg ttattcaaga tatcatcgct gcgctctggc
    41941 aacacgactt tgccgcgctg gcggatcctc atattgttag cgttgtttac tttgtcatgt
    42001 ttgccacgct gtttttagaa aacggcctgc tgcccgcctc atttttgcca ggcgacagct
    42061 tgttgatact ggcaggcgca ttgattgccc aaggggttat ggattttctg cctacgattg
    42121 cgattctgac cgccgcagca agtctgggct gctggctaag ttatattcag gggagctggt
    42181 tagggaatac caaaacggtg aaaggctggc tggcacagct tcctgctaaa tatcaccagc
    42241 gcgccacctg catgtttgac cgccacggtc tgctggcgct gctggctgga cgttttcttg
    42301 catttgtccg tacgctgctg ccaaccatgg cgggaatttc cggtctgcca aaccgccgct
    42361 tccagttttt caactggtta agtggattgc tgtgggtcag cgtggtaacc agttttggct
    42421 atgccttaag tatgattccg ttcgttaaac gccatgaaga tcaggtaatg acgttcctga
    42481 tgatcctgcc aattgccttg ttaaccgctg gcttgttagg cacgctgttt gtggtgatta
    42541 aaaaaaaata ctgtaacgcc tgacgatttt ccccgttccc ggctgctgta ccgggaacgt
    42601 atttaattcc cctgcatcgc ccgcattctt gccgcatctt cccccggcgt cacaccgaag
    42661 taacgtttaa actcacggct aaattgcgat gcgctttcat agccgacgcg catcgctgct
    42721 gcgctggcct tcatgccgtc atggatgatc atcatccgcg ccttatgcag acggtaattc
    42781 ttcaaatact gcaacggcga ggtgctggtg acagacttaa aattatggtg gaacgccgat
    42841 acgctcatgt tggcttctgc cgccagttgc tcgacgctca ggttttcggt gtatttattc
    42901 tcaattcgtt tcagcacgcg gctaatcaga ctgaagtgag tctggcgact gaccagcgcc
    42961 agtaacgcgc cgccgcaagg tccggtcagc acgtagtaca gaatttcgcg gatgatctgt
    43021 ttgccgagaa tacgcgcatc cagtggtcgc tccatcacgt cgagtaaccg ctccgccgcg
    43081 cataaaatct cttctgataa cgtggcggag ttaatcccgc tggctgccat cgacggctgg
    43141 aaatgctcat cttcgccaat gtccatcaac agttcctgta actgcaaaat atcgacattg
    43201 agacgcaacc ctgccagcgg cacctctgac gtggcatagg tttcgcactc aaacggcaac
    43261 ggcaccgtca gcagcaggta ttcattggca tcataacgaa acacgcgttc attgatataa
    43321 ccgattttat gcccggaaaa gagaattatg atgccaggct cgtacatcac cggtgtacgt
    43381 gcgaaaggcg tctcgccata caacaaacgc acatcgggca acagttctga caaactattt
    43441 tctttatttt tcagtttatt aactttatcc gccagcaagc gacaaatctc ttcacgtttc
    43501 atatcgcgta atttcttagg aataatgcgg caatttgatt gtgcgcaatt ttgtagcatt
    43561 tctccagcac tctggagaaa taggcaagac attggcagaa atgagcattg agagccaggg
    43621 cgctggcgat cacaatgaaa aacatcaggc agatcgttct ctgccctcat attggcccag
    43681 caaagggagc aagtaatgaa caactttaat ctgcacaccc caacccgcat tctgtttggt
    43741 aaaggcgcaa tcgctggttt acgcgaacaa attcctcacg atgctcgcgt attgattacc
    43801 tacggtggcg gcagcgtgaa aaaaaccggc gttctcgatc aagttctgga tgccctgaaa
    43861 ggcatggacg tgctggaatt tggcggtatt gagccaaacc cggcttatga aacgctgatg
    43921 aacgccgtga aactggttcg cgaacagaaa gtgactttcc tgctggcggt tggcggcggt
    43981 tctgtactgg acggcaccaa atttatcgcc gcagcggcta actatccgga aaatatcgat
    44041 ccgtggcaca ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc gatgggctgt
    44101 gtgctgacgc tgccagcaac cggttcagaa tccaacgcag gcgcggtgat ctcccgtaaa
    44161 accacaggcg acaagcaggc gttccattct gcacatgttc agccggtatt tgccgtgctc
    44221 gatccggttt atacctacac cctgccgccg cgtcaggtgg ctaacggcgt agtggacgcc
    44281 tttgtacaca ccgtggaaca gtatgttacc aaaccggttg atgccaaaat tcaggaccgt
    44341 ttcgcagaag gcattttgct gacgctaatc gaagatggtc cgaaagccct gaaagagcca
    44401 gaaaactacg atgtgcgcgc caacgtcatg tgggcggcga ctcaggcgct gaacggtttg
    44461 attggcactg gcgtaccgca ggactgggca acgcatatgc tgggccacga actgactgcg
    44521 atgcacggtc tggatcacgc gcaaacactg gctatcgtcc tgcctgcact gtggaatgaa
    44581 aaacgcgaga ccaagcgcgc taagctgctg caatatgctg aacgcgtctg gaacatcact
    44641 gaaggttccg acgatgagcg tattgacgcc gcgattgccg caacccgcaa tttctttgag
    44701 caattaggcg tgccgaccca cctctccgac tacggtctgg acggcagctc catcccggct
    44761 ttgctgaaaa aactggaaga gcacggcatg acccaactgg gcgaaaatca tgacattacg
    44821 ttggatgtca gccgccgtat atacgaagcc gcccgctaag ctttttacgc ctcaaacttt
    44881 cgttttcggg catttcgtcc agacttaagt tcacaacacc tcaccggagc ctgctccggt
    44941 gagttcatat aaaggaggaa cgtatggcta atccaaccgt tattaagcta caggatggca
    45001 atgtcatgcc ccagctggga ctgggcgtct ggcaagcaag taatgaggaa gtaatcaccg
    45061 ccattcaaaa agcgttagaa gtgggttatc gctcgattga taccgccgcg gcctacaaga
    45121 acgaagaagg tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa gaactgttca
    45181 tcaccactaa gctgtggaac gacgaccaca agcgcccccg cgaagccctg ctcgacagcc
    45241 tgaaaaaact ccagcttgat tatatcgacc tctacttaat gcactggccc gttcccgcta
    45301 tcgaccatta tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag ggattaatca
    45361 aaagcatcgg cgtgtgcaac ttccagatcc atcacctgca acgcctgatt gatgaaactg
    45421 gcgtgacgcc tgtgataaac cagatcgaac ttcatccgct gatgcaacaa cgccagctac
    45481 acgcttggaa cgcgacacac aaaatccaga ccgaatcctg gagcccatta gcgcaaggag
    45541 ggaaaggcgt tttcgatcag aaagtcattc gcgatctggc agataaatac ggcaaaaccc
    45601 cggcgcagat tgttatccgc tggcatctgg atagcggcct ggtggtgatc ccgaaatcgg
    45661 tcacaccttc acgtattgcc gaaaactttg atgtctggga tttccgtctc gacaaagacg
    45721 aactcggcaa aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat cctgaccagt
    45781 tcggcggcta acgtgcaaat tctcccggtg gcggtaatgt tccgctaccg gacttttcag
    45841 aaatcattta ttcccctcgc gtcccgcccg ttgttactct tccttgttca ggaatgccaa
    45901 atataaggac atcatcatgc agagccggaa gctcttaaaa gaacaactca tctatatccg
    45961 ggataaacgc aacggagagg tgaaaaacag atgaaaataa tacttctgtt tttagcagcc
    46021 ctggcaagtt ttaccgtaca cgcgcagcct ccttcacaga ccgtagaaca aacagtccgg
    46081 cagatttatc agaactataa atcagatgcc agtacgcctt atttcggtga aaccggagag
    46141 cgggcgataa cttcagcgcg tattcaacag gcgcttaccc tgaacgacaa tcttacacta
    46201 ccgggcaata ttggctggct ggattatgat ccggtttgtg attgtcagga ttttggcgat
    46261 ctggtgttag aaagcgtagc gataacccaa actgacgtcg atcatgccga tgccgttgtg
    46321 cgctttcgta tctttaaaga tgataaagaa aagaccacac agacactgaa aatggtggcg
    46381 gaaaatggtc gttgggtcat tgatgatatt gtcagcaatc atggcagcgt cttacaagca
    46441 gttaatagcg agaatgaaaa aacgctggcc gctttagctt cgttgcagaa agaacagcca
    46501 gaatcctttg ttgctgaact ctttgaacat attgctgatt atagctggcc gtggacgtgg
    46561 gtggtttccg actcttaccg ccaggcggtt aatgccttct ataaaaccac cttcaagacg
    46621 gccaataatc ccgatgaaga tatgcaaata gaacggcaat ttatttacga caatccgatc
    46681 tgttttggcg aagagtcgct attttcacgc gttgatgaaa ttcgagtcct ggagaaaacc
    46741 gccgattccg cccgcattca tgttcgtttt acgctgacca atggcaacaa tgaagagcaa
    46801 gagctggttt tacagcggcg cgaaggtaag tgggaaatcg ctgattttat ccgcccgaac
    46861 agcggcagcc tacttaagca gattgaggca aaaaccgccg ccagattaaa gcaatgagct
    46921 gaattaaata acaattagcc ggaacaataa ataaaaggga acactatatg aaaacgattt
    46981 tcaccgtggg agctgttgtt ctggcaacct gcttgctcag tggctgcgtc aatgagcaaa
    47041 aggtcaatca actggcgagc aatgtacaaa cattaaatgc caaaatcgcc cggcttgagc
    47101 aggatatgaa agcactacgc ccacaaatct atgctgccaa atccgaagct aacagagcca
    47161 atacgcgtct tgatgcacag gactattttg attgcctgcg ctgcttgcgt atgtacgcag
    47221 aatgataaaa aaatccccgg cagcatgtca gttgccgggg atttttttta acgtccaacc
    47281 gccgctttag ggcgtttctt cgcaccagca ttcaccggac gagattgcgt agacgacgct
    47341 ttttttgccg tagcaggcgt ctgacgctga gtcgccatcg gcgtatgttt agtcaacgcc
    47401 ggacgggtat tgcggttctg gcgacgagct tcacgcatct cttcaatggt tggcgcaggc
    47461 actaagcaat cgcgacggct gccaatcaga tgctttttgc ccatcgcttc cagcgcctgg
    47521 cggattaacg gccagtttgc cggatcgtgg taacgcaaca acgctttatg caaacgacgc
    47581 tgtttgtcgc ccttcggtac gaagacgcct tcgctcttat aaccaatctt cgccagcggt
    47641 tttttcccgg tgtaatacat ggtggttgag ttcgccagcg gcgatgggta gaagttctgt
    47701 acctggtcga gacggaaacg atgctttttc agccacagcg ccaaattcac catatcttca
    47761 tcacgcgtac cggggtgcgc agagatgaaa tacgggatca gatactgctc tttacctgcc
    47821 tgtttcgagt aggtatcgaa cagctcttta aagcggtcat agctgcccat gcccggcttc
    47881 atcatcttcg acaacggccc ttcttcggta tgttccggag caatcttcag ataaccgccg
    47941 acgtgatggg tcgccaactc tttgatatag cgcggatctt ccacggcgat gtcataacgt
    48001 acgcctgagg cgataaggat ttttttaatg cctttcagat cacgcgcacg gcgatagagg
    48061 ttgatcgtcg gttcgtggtt agtgtccatg tgcggacaaa tatccggata aacgcacgac
    48121 aaacggcgac aagtttgttc agcgcgtggc gatttgcagc gcaacatata catgttggca
    48181 gttggcccac cgagatcgga aatcacgccc gtaaaacctg gaacggtgtc gcggatcgct
    48241 tcgatctcat taatgatcga atcttccgaa cggctctgaa taatgcgccc ttcgtgctcg
    48301 gtaatagaac agaaagaaca gccgccaaag cagccgcgca taatgttgac cgaaaaacgg
    48361 atcatttcgt aagccggaat acgggcattg ccataggccg gatgtggcac gcgcttgtac
    48421 ggcagcgcaa aaacgctgtc catctcttcg gtagaaagcg ggatagcagg cgggttgatc
    48481 cacacatagc ggtcaccgtg tttttgcatc aatgcgcggg cgcagcctgg gttggtttcg
    48541 tggtgcagaa tgcgcgaagc atgggcgtac agcactttat cgcccttcac tttctcgaaa
    48601 gaaggcagca atacgtaggt tttttcccac ggtttctggc gcggtggctg cacgattaca
    48661 gctttcgctt cctgcttttt cggcgcaaca ggtttgttat ccgcacacgg caaatcttcg
    48721 ccatacggat gcgggattgg gtcgattttc cctagggtat caagacgggt ggaatccaca
    48781 ccgctccagc caggcagcgc ctcttttacg ataattgcgg tattacgcac atcgcggatt
    48841 tcactaatcg gctcgcccat cgccagacga tgcgctactt ccaccagcgg acgttcaccg
    48901 ttaccaaaca tcagcatgtc ggctttcgaa tctaccagaa cggaacggcg cacggtatcg
    48961 gaccagtaat cataatgcgc ggtacggcgc aggctagcct caataccgcc gaggatcacc
    49021 ggcacatctt tccacgcctc tttacaacgc tgggtataaa ccagcgtggc gcgatccggt
    49081 cgcttacccg cgacattatc tggcgtgtag gcatcatcat gacgtaaacg gcgatcggcg
    49141 gtatagcggt tgatcatcga atccatgttg ccagcagtaa caccaaaaaa cagattcggt
    49201 ttacccagac gcataaagtc gtctttgctg ctccagtccg gctgggcgat gatcccgacg
    49261 cgaaagccct gcgcttccag catacgaccg caaatcgcca tcccgaagct tgggtgatcg
    49321 acatacgcgt cgccagtaac caaaatgatg tcgcagctat cccagccaag ttgatccatc
    49381 tcttcacgag acatcggcaa aaacggtgcc ggtccaaaac aggcggccca gtactgcggc
    49441 caggagaaca ggtcgcgatc cggttggatc agggagatag agctcatttt gcttccagaa
    49501 atgataaaaa aataatcaaa ggccggggat tataagccgg aacgaaagag aaatcgaaag
    49561 gtattccata ctcgccctcc tcgggcgagt atgaagatta cggtaccgga ttgaccaaca
    49621 gttgcccaat cgagccacgg tccgccattt ccagcgtctg actgttgaag tagaacggga
    49681 agtgcgccca ggaaggctga ccgaaataaa caagcagctc cacttgtccg tcaacccaaa
    49741 cggtatcttt ccagcctctg tcttccggga acggcatcgc gccattcacg ttacggatct
    49801 ggaacattac gccttcaata tggaacgctt gcggctcgtc cgcgcgtacc gtccagcgtt
    49861 cccacgttcc ttgctgcgcg gtgacatcaa tacggttgac gtcccacagc tgtccattaa
    49921 tacccgggtc atcacccaga ctgatatcgc gactgcgaat tggcgaacca gccatgattt
    49981 cagttggcag caagcgcatc ggaagactgt ctgtgaccag cggcagaagg ccggttgggc
    50041 gcagcgttag caccaaggta gaaaccagaa tactggatgg ctcaaagaag ccgcgaatac
    50101 gatcaacaat gctcgccgct tcaccacagg tgatcgacac ttcatcgccg ttgctcatat
    50161 ccaccagaat ctcgcggcgc tcgcccggtg ccagcgaaag ttgcttcacc gatacaggag
    50221 caggcaggaa tccctgatcg ccagaaatca catgtaacgg gcgaccatcg ctcatctgta
    50281 gttgatagcg acgagagttc gacgcgttca gcagtcgcaa gcgcacccag ccacgcgaga
    50341 cttcaacgta cgggctttgt acaccgttaa ccagcagcgt atcaccaaca aagccgccgc
    50401 ttcccggttc gttgtattct ggcgtaccaa agttatccag ccgtttatcc tggataatga
    50461 ccggaaaatc atccacacca taatggttgg ggataggcag cgacttgctg acttcatctt
    50521 ccaccagcca cattccggca aggccgttat agacctgctg agccgtgcgg ttgggagtat
    50581 tggcgtgata ccacagagta gctgcgttct ggcgaatagg cagtacgggt gcccagtcag
    50641 cgtttggcga catcatccgt gccggaccgc ccatcagcgg gcctggtacc tgtagcccgg
    50701 ccaccgtcat tgagacattt tctgtcaggc ggttgctgta aataagctta acatcgtcgc
    50761 ccttccagac gcggatagtc ggccccaggt aacgaccatt gattccccag accgacgcgc
    50821 gtgtccctgg cgtaaatgac cagtgcgcac gttgtacagt cataaacagc ggttgcccac
    50881 ggcgagattc aagtagcggc ggaacgggta gcggttgctg ttgcccggct gcgctggcct
    50941 tcaggggaac agcgcctgca caaagtgcaa tccccgatgc ctgaatgaac tgacgccgac
    51001 tgagtgacat atttgctcca tgtaaaactg gctaaagtaa taccgtgcag gaaagtgttc
    51061 cccgcaaaaa tacagattgc cgacttaaac ttttccggcg gcttcgcgtt ctgcgacttc
    51121 tttatcgagc tcggcgattt tttgttccat tatcgaacga caatgggcag ccagctcacg
    51181 aacctgatct ttgccatact gactgacgtc aattggcggc agcatttcga caatcaccag
    51241 accgttgtgc agtcgattaa gattaatctt attcgaagtt gtagagacgc acacgggaat
    51301 aatcgggacg cccgccgcaa ttgccgcgtg aaatgctcca gtcttgaacg gtagcaggcc
    51361 gcgaccacgg ctgcgggttc cttccgggaa catccagatg gaaatacggc gttttttgaa
    51421 atgattcacg acttccgcaa tggtgccgtg agctttagtg cgattgtttc tgtcgatcaa
    51481 taagttgccg gttaaccagt acaactgccc gaagaagggg atccacagca agctcttttt
    51541 acctaccgtc accgtcggcg gttgcacaat gttcgatgct gtcaccatgt catagttgtt
    51601 ctggtggtta gcgatataga tagcattgcc gtagctttca gcatctgcag gtttgcggca
    51661 ctcaactttc aggccgaaca gtggcgcaag acggccaaac atatgcccaa aggtggccac
    51721 atgtttcggg ttacgcgggc tgaaaaggca gtaaatggag ccgaatacac agactaagat
    51781 gctgtaaatc acggtaataa taagacgaaa gatatatagc ataacaccct ctgaaggtct
    51841 gagagactgg cattgtacgt cacctgaaat caggttaggg cattgttaaa agccgctctc
    51901 tggaaaagcg tattgttaat cgcaacgcat gaataaacaa cggtttcaag ggaattttta
    51961 tcgtttccgg gggttgaata cgctgccgga tgacgactta acgtttcatc cggcgttcct
    52021 tgctagcggg aggaaacagc gccctccccg gcatattact cttcgctatc accgctgctg
    52081 gcacggcgag gagagtcgat ctcaacacga tcgatacgct gcaaaccgcg catcaacgta
    52141 ccgcggcgtc cacgttcgcc agtgactttc tgtagctctt ccggacgcag tttaattttg
    52201 cgtttcccaa catgaatggt cagcgtgctt tgcggcggca gaacgtacag ttgcgccaga
    52261 ccatcctcgc cacgcgcggc ttctgccgat ggaatgttga taatcttgtt gcctttgccc
    52321 ttcgacagct gcggcagatc gcttaccggg aacatcaaca tacggcctgc ctgagtgatt
    52381 gccagcagca tatcggaagc atcttcaatc accaccggcg gcataacatg ggcattttcc
    52441 ggtaaggtga tcaaagcctt acccgcacgg ttacgcgcta ccagatcgtt aaaggtgcag
    52501 acgaaaccgt aacccgcatc ggaagccatc agcagtttct gatcgtcgct ttccatcagc
    52561 atatggtcaa cggtcgcccc aggcggcaac gttaatttgc cggtgagtgg ttcgccctga
    52621 ccacgcgccg acggcagcgt aatcgggtcg atggcatagc tacgaccggt ggaatcaaca
    52681 aacactaccg gttggttact cttacctttc accgccgctt tgaagctatc acccgcttta
    52741 taattcaggc ccggcgcgtc gatatcatgg cctttagcgc tgcgtaccca gcccatctgc
    52801 gacagcacaa tggtgacagg ttcagacggc agcatgtcgt gctcgctcat cgctttcgct
    52861 tcttcgcgtt cctgcaacgg cgaacgacga tcgtcaccgt aggcttgcgc gtctgcctgc
    52921 agttctttct tcagcaggtt attcatttta cgctcggaag ccaaaatgcc ctgcaactgg
    52981 tcgcgctctt tttccagctc actctgctca ccgcgaatct tcatctcttc cagtttggca
    53041 agatgacgca gtttcagttc gaggatcgct tccgcctggg tttccgtaag gccaaaccgc
    53101 gacatcagcg ccggtttcgg ttcatcttcg ttacgaatga tctcaatcac ttcgtcgatg
    53161 ttgagaaacg ccaccagcaa accttccagg atatgcaggc gcttgaggac tttctccaga
    53221 cgatagttca gtcggcggcg cacggtatcg cgacggaaca ccagccattc ggagaggatt
    53281 tccagcaggt ttttcaccgc cggacgacca tccagaccga tcatattgag gttaatgcga
    53341 tagctctttt ccagatcggt ggtagcgaag aggtggttca tcacctgatc catatccacg
    53401 cggttggaac gcggcacaat caccagacgg gtcgggttct cgtggtcaga ttcatcgcgc
    53461 agatcgtcaa ccatcggcag ctttttgttg cgcatttgcg cagcaatttg ctccagtacg
    53521 cgcgcacctg aaacctgatg cggtaatgcg ctgataacca ccgcgccatc ttctttcttc
    53581 cacaccgcgc gcatacgcac tgaaccacgt ccgttctcgt agattttacg aatctcggcg
    53641 cgcgaagtga taatttccgc ttcagtcgga taatccggcc cctgcacgat atccagcagc
    53701 tgatcgagcg tagttttcgg ctggtcgatt aatgcgattg ccgcctgagc tacttcacgc
    53761 aggttatgcg gtggaatatc ggtcgccatg ccgacggcaa taccggtggt gccgttaagc
    53821 aaaatgtttg gcagacgggc aggtagcatt ttcggctcct gcaaagtgcc gtcgaagttt
    53881 ggcacccagt cagccgtccc ctgccccagc tcgctcaata gcagctcgga atatttcgac
    53941 aaccgggatt cggtgtaacg cattgccgcg aacgatttcg gatcgtccgg cgcgccccag
    54001 ttcccctgac catcaaccag cggataacgg taagagaacg gctgcgccat caggaccatc
    54061 gcttcataac aggcgatatc gccgtgcgga tggtatttac ccagtacgtc accgacggta
    54121 cgggccgatt ttttaaattt ggcgctggca ttcaggccca gttcagacat cgcatacaca
    54181 atgcggcgct gaacaggttt cagaccatca ccaataaacg gcaacgcacg gtccatgatc
    54241 acgtacatgg agtagtttaa gtaggcgttt tccgtaaatt catgtagcgc aaggcgctct
    54301 gccatatcgc tcattaattc tgattcctca acttattcgc cagccttcaa aggggcaata
    54361 ttgccggaga tactacatta tctgccgcgt cgagtcacag gagatcagcg tgaatagcgc
    54421 cgccggacgt gctccgacgg cgataaaggc atcaatgtgc cttgatatac aactctttgc
    54481 tgtagacttg atcggcatag agggcagcac aaggttgcgc ccatgccaca ccactcggca
    54541 tgggggtccg ctccgggcga ttcgagaagt tgaggttatg cgagtccagg gaagcctaaa
    54601 gcatcaaagt agtcgactgt aagtatacgg ttcagcccag tttagctgtg tctccaccag
    54661 tgccccgcgc ctcctgccat aagtgcggct tgctcatgga tatccccgag acggcttacg
    54721 gcgcaccgga taataaattt cgcgtataca tggtattcct taccggagaa attataagcc
    54781 caacaacaat acgcctgtga cattgttcgc tcaaacttat cgcgagtgat ttggctcaca
    54841 ctccggtaaa gattccccat cacgccaacg gcacataaac atccgtttcc agtcgctgct
    54901 ctgtcacccc ttcggcaaga ttggtgtagt gaaacagaat cggtgcttta cgcattttct
    54961 cgccgcttgc aggcagccag tggcgaataa tgccccatat cgtgtgtgaa atatcgtcca
    55021 gctcgccaac gtggcgggcc acggcataac gtccaccggt aagttcacca ttgctcacac
    55081 cataacgatt atcgggaatc ggttcgctaa cgctgccgca gatatcaaag cgaaacgctt
    55141 ccggtgcggt ggtggcaggg gcgtcccagg cgacgccaaa agtctggctt tggttaacag
    55201 gagaaagccc cgtctcttta cgccacatga taaatttcgc cacactgtag ttgagcaact
    55261 cagggctaca gcggtgcggc aacatcgcca cagggattga tgggaaatcg ataatgttta
    55321 catccagtgt caggtttgtc attttgcctc cctgcgaaat tattcccggg aacgcgtccc
    55381 gggagcggta acaattacgg atttactttg cggatctgtt tgacgtcaat ttcaacagaa
    55441 ttccagtctt tatcgacttc a
//
LOCUS       DAZSNM010000004        54435 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZSNM010000004 DAZSNM010000000
VERSION     DAZSNM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54435
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.004"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            52..684
                     /gene="comR"
                     /locus_tag="ACHW42_000164"
     CDS             52..684
                     /gene="comR"
                     /locus_tag="ACHW42_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR8942159.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(746..1285)
                     /locus_tag="ACHW42_000165"
     CDS             complement(746..1285)
                     /locus_tag="ACHW42_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR8942160.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1512..2816)
                     /gene="ndh"
                     /locus_tag="ACHW42_000166"
     CDS             complement(1512..2816)
                     /gene="ndh"
                     /locus_tag="ACHW42_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR8942161.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3224..3766)
                     /gene="ycfP"
                     /locus_tag="ACHW42_000167"
     CDS             complement(3224..3766)
                     /gene="ycfP"
                     /locus_tag="ACHW42_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR8942162.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3789..4814)
                     /gene="nagZ"
                     /locus_tag="ACHW42_000168"
     CDS             complement(3789..4814)
                     /gene="nagZ"
                     /locus_tag="ACHW42_000168"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR8942163.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(4825..5649)
                     /gene="thiK"
                     /locus_tag="ACHW42_000169"
     CDS             complement(4825..5649)
                     /gene="thiK"
                     /locus_tag="ACHW42_000169"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR8942164.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5630..6271)
                     /gene="lpoB"
                     /locus_tag="ACHW42_000170"
     CDS             complement(5630..6271)
                     /gene="lpoB"
                     /locus_tag="ACHW42_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR8942165.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6286..6663)
                     /locus_tag="ACHW42_000171"
     CDS             complement(6286..6663)
                     /locus_tag="ACHW42_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR8942166.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6666..7025)
                     /gene="hinT"
                     /locus_tag="ACHW42_000172"
     CDS             complement(6666..7025)
                     /gene="hinT"
                     /locus_tag="ACHW42_000172"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR8942167.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7358..9543
                     /gene="fhuE"
                     /locus_tag="ACHW42_000173"
                     /pseudo
     CDS             7358..9543
                     /gene="fhuE"
                     /locus_tag="ACHW42_000173"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(9603..11036)
                     /gene="ptsG"
                     /locus_tag="ACHW42_000174"
     CDS             complement(9603..11036)
                     /gene="ptsG"
                     /locus_tag="ACHW42_000174"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR8942168.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11331..12128)
                     /locus_tag="ACHW42_000175"
     CDS             complement(11331..12128)
                     /locus_tag="ACHW42_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR8942169.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12139..13143)
                     /gene="holB"
                     /locus_tag="ACHW42_000176"
     CDS             complement(12139..13143)
                     /gene="holB"
                     /locus_tag="ACHW42_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR8942170.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13140..13781)
                     /gene="tmk"
                     /locus_tag="ACHW42_000177"
     CDS             complement(13140..13781)
                     /gene="tmk"
                     /locus_tag="ACHW42_000177"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR8942171.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13771..14793)
                     /gene="yceG"
                     /locus_tag="ACHW42_000178"
     CDS             complement(13771..14793)
                     /gene="yceG"
                     /locus_tag="ACHW42_000178"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR8942172.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14796..15605)
                     /gene="pabC"
                     /locus_tag="ACHW42_000179"
     CDS             complement(14796..15605)
                     /gene="pabC"
                     /locus_tag="ACHW42_000179"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR8942173.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(15725..16966)
                     /gene="fabF"
                     /locus_tag="ACHW42_000180"
     CDS             complement(15725..16966)
                     /gene="fabF"
                     /locus_tag="ACHW42_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR8942174.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17053..17289)
                     /gene="acpP"
                     /locus_tag="ACHW42_000181"
     CDS             complement(17053..17289)
                     /gene="acpP"
                     /locus_tag="ACHW42_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR8942175.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17500..18234)
                     /gene="fabG"
                     /locus_tag="ACHW42_000182"
     CDS             complement(17500..18234)
                     /gene="fabG"
                     /locus_tag="ACHW42_000182"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR8942176.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18247..19176)
                     /gene="fabD"
                     /locus_tag="ACHW42_000183"
     CDS             complement(18247..19176)
                     /gene="fabD"
                     /locus_tag="ACHW42_000183"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR8942177.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19192..20145)
                     /gene="fabH"
                     /locus_tag="ACHW42_000184"
     CDS             complement(19192..20145)
                     /gene="fabH"
                     /locus_tag="ACHW42_000184"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR8942178.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20213..21283)
                     /gene="plsX"
                     /locus_tag="ACHW42_000185"
     CDS             complement(20213..21283)
                     /gene="plsX"
                     /locus_tag="ACHW42_000185"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR8942179.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21364..21537)
                     /gene="rpmF"
                     /locus_tag="ACHW42_000186"
     CDS             complement(21364..21537)
                     /gene="rpmF"
                     /locus_tag="ACHW42_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR8942180.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21589..22110)
                     /gene="yceD"
                     /locus_tag="ACHW42_000187"
     CDS             complement(21589..22110)
                     /gene="yceD"
                     /locus_tag="ACHW42_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR8942181.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22309..22893
                     /gene="yceF"
                     /locus_tag="ACHW42_000188"
     CDS             22309..22893
                     /gene="yceF"
                     /locus_tag="ACHW42_000188"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR8942182.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23005..23964)
                     /gene="rluC"
                     /locus_tag="ACHW42_000189"
     CDS             complement(23005..23964)
                     /gene="rluC"
                     /locus_tag="ACHW42_000189"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR8942183.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24537..27728
                     /gene="rne"
                     /locus_tag="ACHW42_000190"
     CDS             24537..27728
                     /gene="rne"
                     /locus_tag="ACHW42_000190"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR8942184.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27925..28867)
                     /gene="flgL"
                     /locus_tag="ACHW42_000191"
                     /pseudo
     CDS             complement(27925..28867)
                     /gene="flgL"
                     /locus_tag="ACHW42_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(28879..30521)
                     /gene="flgK"
                     /locus_tag="ACHW42_000192"
                     /pseudo
     CDS             complement(28879..30521)
                     /gene="flgK"
                     /locus_tag="ACHW42_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(30587..31528)
                     /gene="flgJ"
                     /locus_tag="ACHW42_000193"
     CDS             complement(30587..31528)
                     /gene="flgJ"
                     /locus_tag="ACHW42_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR8942185.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31528..32625)
                     /gene="flgI"
                     /locus_tag="ACHW42_000194"
     CDS             complement(31528..32625)
                     /gene="flgI"
                     /locus_tag="ACHW42_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR8942186.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32637..33335)
                     /gene="flgH"
                     /locus_tag="ACHW42_000195"
     CDS             complement(32637..33335)
                     /gene="flgH"
                     /locus_tag="ACHW42_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR8942187.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33388..34170)
                     /gene="flgG"
                     /locus_tag="ACHW42_000196"
     CDS             complement(33388..34170)
                     /gene="flgG"
                     /locus_tag="ACHW42_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFR8942188.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34308..34895)
                     /gene="flgF"
                     /locus_tag="ACHW42_000197"
                     /pseudo
     CDS             complement(34308..34895)
                     /gene="flgF"
                     /locus_tag="ACHW42_000197"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(34997..35059)
                     /locus_tag="ACHW42_000198"
                     /pseudo
     CDS             complement(34997..35059)
                     /locus_tag="ACHW42_000198"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35079..36218)
                     /locus_tag="ACHW42_000199"
     CDS             complement(35079..36218)
                     /locus_tag="ACHW42_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR8942189.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(36243..36938)
                     /gene="flgD"
                     /locus_tag="ACHW42_000200"
     CDS             complement(36243..36938)
                     /gene="flgD"
                     /locus_tag="ACHW42_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR8942190.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(36950..37353)
                     /gene="flgC"
                     /locus_tag="ACHW42_000201"
                     /pseudo
     CDS             complement(36950..37353)
                     /gene="flgC"
                     /locus_tag="ACHW42_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(37357..37773)
                     /gene="flgB"
                     /locus_tag="ACHW42_000202"
     CDS             complement(37357..37773)
                     /gene="flgB"
                     /locus_tag="ACHW42_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR8942191.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            37928..38587
                     /gene="flgA"
                     /locus_tag="ACHW42_000203"
     CDS             37928..38587
                     /gene="flgA"
                     /locus_tag="ACHW42_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR8942192.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            38663..38956
                     /gene="flgM"
                     /locus_tag="ACHW42_000204"
     CDS             38663..38956
                     /gene="flgM"
                     /locus_tag="ACHW42_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR8942193.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            38961..39377
                     /gene="flgN"
                     /locus_tag="ACHW42_000205"
     CDS             38961..39377
                     /gene="flgN"
                     /locus_tag="ACHW42_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR8942194.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(39417..40952)
                     /gene="murJ"
                     /locus_tag="ACHW42_000206"
     CDS             complement(39417..40952)
                     /gene="murJ"
                     /locus_tag="ACHW42_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR8942195.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41119..41985)
                     /locus_tag="ACHW42_000207"
                     /pseudo
     CDS             complement(41119..41985)
                     /locus_tag="ACHW42_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(41987..42634)
                     /locus_tag="ACHW42_000208"
     CDS             complement(41987..42634)
                     /locus_tag="ACHW42_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HFR8942196.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42645..43229)
                     /gene="rimJ"
                     /locus_tag="ACHW42_000209"
     CDS             complement(42645..43229)
                     /gene="rimJ"
                     /locus_tag="ACHW42_000209"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR8942197.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43465..44673
                     /gene="mdtH"
                     /locus_tag="ACHW42_000210"
     CDS             43465..44673
                     /gene="mdtH"
                     /locus_tag="ACHW42_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR8942198.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44737..45384
                     /gene="grxB"
                     /locus_tag="ACHW42_000211"
     CDS             44737..45384
                     /gene="grxB"
                     /locus_tag="ACHW42_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR8942199.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            45518..46078
                     /locus_tag="ACHW42_000212"
     CDS             45518..46078
                     /locus_tag="ACHW42_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR8942200.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46184..47230
                     /gene="pyrC"
                     /locus_tag="ACHW42_000213"
     CDS             46184..47230
                     /gene="pyrC"
                     /locus_tag="ACHW42_000213"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR8942201.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47304..47549
                     /gene="dinI"
                     /locus_tag="ACHW42_000214"
     CDS             47304..47549
                     /gene="dinI"
                     /locus_tag="ACHW42_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR8942202.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47839..48093
                     /gene="bssS"
                     /locus_tag="ACHW42_000215"
     CDS             47839..48093
                     /gene="bssS"
                     /locus_tag="ACHW42_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR8942203.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48208..49326
                     /gene="solA"
                     /locus_tag="ACHW42_000216"
     CDS             48208..49326
                     /gene="solA"
                     /locus_tag="ACHW42_000216"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR8942204.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            49374..49487
                     /locus_tag="ACHW42_000217"
     CDS             49374..49487
                     /locus_tag="ACHW42_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFR8942205.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            49748..50314
                     /locus_tag="ACHW42_000218"
     CDS             49748..50314
                     /locus_tag="ACHW42_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR8942206.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            50318..50893
                     /locus_tag="ACHW42_000219"
     CDS             50318..50893
                     /locus_tag="ACHW42_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR8942207.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(50935..51987)
                     /locus_tag="ACHW42_000220"
     CDS             complement(50935..51987)
                     /locus_tag="ACHW42_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR8942208.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            52212..53132
                     /gene="lpxL"
                     /locus_tag="ACHW42_000221"
     CDS             52212..53132
                     /gene="lpxL"
                     /locus_tag="ACHW42_000221"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR8942209.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            53304..53489
                     /locus_tag="ACHW42_000222"
                     /pseudo
     CDS             53304..53489
                     /locus_tag="ACHW42_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            53525..>54435
                     /locus_tag="ACHW42_000223"
     CDS             53525..>54435
                     /locus_tag="ACHW42_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR8942210.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
BASE COUNT        13301 a        14492 c        13422 g        13220 t
ORIGIN      
        1 gattattttt ataataacct ttcattcaca gtcaccaggt acaacggcat catggcaact
       61 gactcaacac aatgtgtaaa aaaaagccgt ggccgcccaa aagtgttcga cagggatgcc
      121 gcgcttgata aggccatgaa attgttctgg caacacggtt atgaagcgac ttctcttgcg
      181 gacctcgtcg aagcaaccgg agccaaagcg cccacgctat acgcggaatt taccaacaaa
      241 gaggggttat ttcgtgccgt tctcgaccgc tatatcgatc gttttgccgc taagcatgaa
      301 gcacagctgt tttgtgaaga gaaaagcgtg gagtctgcgc tggctgacta ttttgctgcc
      361 atcgccaact gctttaccag caaagacacc ccggcaggct gcttcatgat caacaactgc
      421 accaccctct ccccagattc aggagatata gccaatacgt tgaaatcacg ccatgcgatg
      481 caagagcgca ctttgcagca gtttttatgt caacgacaag cgcgcgggga aatcccgacc
      541 cactgtgacg tgacacatct ggcagaattc cttaattgta ttattcaggg gatgtcgatc
      601 agcgcacgcg aaggtgcatc gctggaaaaa ctgatgcaga ttgccggaac gactttgcgt
      661 ttatggcccg aactggtgaa ataagtcgca aaaaaggccc ctcagcctga gcgaatgagg
      721 ggccaaattg cagagtacag ccttgttata ctttgttatt caaaatcagt tgcccgttac
      781 tatctagcgg gatctgcgta cccggatcgc ggtccatgcg gattttgccc tgctgatcgc
      841 caatcttata ggtcacatca taaccgagca ttttttctga cttgtcatac accgttttac
      901 aacgctgttg cgtagtcgtg taagtatcgc tttcctggag agagccctgg atctggttac
      961 cggcatatcc accacccagc gcccccacaa cagtggcgac atctttaccg cgaccaccac
     1021 caaactgatg ccctatcacg ccaccagcaa cagcgccgag caccgacccg gtaatgcgat
     1081 tttcatcctg caccggtcga cgatgggtca ctgtgacgtt gcgacactcc tgacgcggtg
     1141 ttttaaccgt ttccttgatt ggggttgcag aaacaacctg agcgtattgc gggccccgtt
     1201 caaacacgtt cagactggcc actgccgcta cgcccaacgc agctgcgaca ccaatcccga
     1261 tacccgccaa cattgattta ttcacgggaa cacctccttc tttgcagtta tgccaatcat
     1321 gcaagcgaag gagaaaagca gctatcagac agaagatgaa aagcgcggga atgactggaa
     1381 atacttaagg attcggagga gtctgaagga atgcctgatg cgacgcttaa cgcgtcttat
     1441 caggcctacc gctcgtacca tttgtccagg gcggataagg cgtttatacc acatccgcca
     1501 gtgtacgtcg attaatgcaa cttcaaacgc ggacggataa cgcggttaat actccccacc
     1561 agcatcatta atccggtttt aaagtaacca tgcagcgcaa tctgatgcat tcggtatagc
     1621 gagatatata caaagcgcgc aattcgtcct tcaatcatca ttgagccgcg cgtcaggtta
     1681 cccatcaggc taccaacggt ggagaagttc gacagcgata ccagcgaacc atgatcttta
     1741 tactgataat ttttcagcgg cttaccgttc atctgcgcca gaatgttgtt cattgcgcaa
     1801 gtcgccatct ggtgtgcagc ctgagcacgc ggcggaacaa agcccccttc cggacgcggg
     1861 catgacgcgc agtcgccaat agcgtaaatg tctggatcgc gagtggtttg cagcgtcggt
     1921 tccaccacca gctggttgat acggttagtt tcaagaccac cgatatcttt caggaagtct
     1981 ggcgctttga tcccggctgc ccataccatc agatcagcct caatatattc gccatcttta
     2041 gtgtgcaggc cgccttcatc agcactggtg accatggttt gcgtcagcac gcgaacgcca
     2101 agtttcgtta gctcgttgtg ggccgcagca gagatacgtg gcggtaatgc aggcaaaata
     2161 cgttctcccg cttctaccag cgttacgttc agggcttcgt tggtcaggcc tttgtaaccg
     2221 tagctgtgca gttgcttgat cgcgttgtgc aattcagcgg agagttctac acccgtcgcg
     2281 ccgccgccga caatcgcaat gttcactttg ccatttgcgc ccaggttggc agagtatttc
     2341 aggaacagat tcagcatctc ctggtggaag cgacgcgcct ggtgcgggtt atcgaggaaa
     2401 atgcagttct ctttgacgcc tggcgtattg aaatcgttag aggtgctacc cagcgccatg
     2461 accagggtgt cataggcgat tttacgttcc ggaaccaaca gttcaccttt ctcgtcgcgt
     2521 agttctgcaa tagtgattgt tttcgcttca cgatcaatat caatgacgga acccaactgg
     2581 aactggaaac catgattgcg cgcatgggcc agatagctca acgcatcgac gccttcatca
     2641 agcgagccag tcgccacttc gtgcagcagt ggtttccaca ggtggctgtg gttacgatcg
     2701 accagcgtaa ttttggcttt tttcttacgt cccagcttat gccccagctg tgttgccatt
     2761 tccagcccac cagcaccgcc gccgacaatc acaatctttt tcaatggcgt agtcaacgtg
     2821 ccccccttaa atttattaac caattgttaa ttaatggtta ttaacatagc tcttaattaa
     2881 caacaggtta cagcaatgaa aatgttcttc gctattgaga ataacatgaa tggtgcattg
     2941 gtcataccaa aattgatgtt aatcaagttt tgttgcttaa aagataggca actttaccgg
     3001 gggagatcaa aaaatagagt ttattgttta ttatttgagc aaattcggaa gggtaatata
     3061 gggtgaagcg tatatcgagg gcgtttggtc gtcctgataa gatgcgcagc atcgcatcag
     3121 gcattgatgc tcgactgtcg gatgcggcgt ggacgcctta tccgacttac agaattcgga
     3181 gcgcatcagg taccggcaag gttacctgat gcgacgaggg catttacccg agggttttga
     3241 acgctttaat gcgctgtaaa tgcggggaga tattcttgaa tttgtgcgtc tgctcttcgt
     3301 cccagacaat ctcgtaataa tgatgcaact cttcagatgt ccgctggctg ttaagtgctt
     3361 catcattacg cgacaaaata accagacaac gatcgcgatt cttctcgcgg aagttggtca
     3421 cacacttagt ggcaatatcg gcatactctt ccgggcgatc aatcttgcct tccatgttct
     3481 cataagggaa caaattaggg ttgaagatca cctggcggat atcgcaaaga aaaccaatcc
     3541 gttccgccca gtatccgccc aggccaacgc cgcaaattag cggacgctcg tcaacgttca
     3601 gttgcaacat tttgtccact tctttaagca gatgctgcat atcatgtttc ggatgccgcg
     3661 tactgtagct tatcaagcgt acatccgggt caataaactg caattgtaag actttctcgt
     3721 ggttacccgg actgttagag tcaaaaccgt gtaaatagat aatcatcgct tcctcacata
     3781 agccagggtt agtgacctac tttctcttcc tgccagcgtt catgtaactg attcagacgg
     3841 gtgctgatcg ctttccagcg agccgagtcc atcagttcct gtcgcgaaaa tgaaccttta
     3901 tgatacaaac gtgtaacacg ttctgccttg atcggtgaca gattatctaa cacgctgacg
     3961 gcccctttac gattattgca gaccaggatc atatcgcaac ccgcatccag tgatgcctgc
     4021 ccgcgttcgg cataactgcc cataatcgcg gcaccttcca tcgataaatc gtcagagaaa
     4081 atcacgccgt caaaccccag ttcctgacgc aaaacggttt tcagccagta gggagaaccg
     4141 ctcgccggac gcggatcaac atcactgtag atcacatgcg caggcataat ggcgtcgagt
     4201 ttattttcgc ggattaagga actgaagacc gacatatctt tagcgcgaat ctccgcttgt
     4261 ggacgtggat cgcacggtgt ttctttgtgt gagtctgccg ttactgcacc gtgtcctggg
     4321 aagtgtttcc cggtcgtttt cattccggct tcatgcatac catcaataaa ccggctggca
     4381 attgccaggg ctttttgtgg atcggcatga taagaacgct cgccaatcgc cgcgctgata
     4441 tgcccgacat ccagcactgg cgcaaagctg atatcaatat ccatagcgat catttcgctg
     4501 gccatcagcc aacccgcctc ttgcgccagt ttgccgccct cttccattcc caacagcgca
     4561 gcaaaggatt gtgctgccgg taagcgggta aaaccttcgc gaaaacgctg cacgcgtcca
     4621 ccttcctgat ctaccgccac caccagatga ttgcgcgatg ctgcgcggat ctggcgcacc
     4681 agttcacgta actgggcagg atcatgatag ttacgcgtaa agagaatcag ccctcccacc
     4741 agcggatgcg ccagtatttc acgctcttcc gcgtccagtt cgtaaccttc gacatccaac
     4801 attactggac ccacactgct ctccttattg ttttattaat agctgccgcc aggtgtcatc
     4861 ggccagcctg ataaattgtt gatcgccggt ttgtcgccag cggtactcaa accaccctcc
     4921 tttgagcatc agcagccagg gaaaccatcg cctgacctga cgccataatt gcgccggata
     4981 aatcttcgcg cgagtggcat agtcattgac caattgccgg tgctgttcag tattttccac
     5041 ccacaccgcc gccagttcca gcgcgatatc accatctcca gcatactccc agtcgatgag
     5101 ttttaaccct gatgcgctat gcactaaatt tccggcgtgg acatccatat gcaatggact
     5161 taagcgtaaa ggtcgtggtt cccgcgcttt gcgcagacgt tttaacattc gcagccaacc
     5221 cacagtccgc cgcgccggat cgctttgctg ccagtacagt tccagtaagg gcaacagcgt
     5281 tattcgccag ccaaaacgtg gttgttgatg tagataatac agcaagcctg ccagttcgtt
     5341 ggtatccggc aaatacgttt ttaccgcacc gggcagatag tcgactacca tccagtcacg
     5401 gagatataaa tgcggcttcg gtgcaatgct tgcgggtagt tgtgataaag cccgatactg
     5461 gcgcaagaac gcggactgcg gcgcatcagg atcgtgcggc tgacgcacaa caaaacgctg
     5521 gccctgatgt tcaatgagaa aactaccgcc actaagccca ctattaaacg tcgtgacggg
     5581 atgaaactgc gggaaaaagc gcgacagcaa ttcgtcgcgc gtgatgggat tattgctgcg
     5641 aaacggcacc tttacctgac cagataattt cgcccgtctg caccagcatc agctgcattt
     5701 gtagggtcgg agcgttaacg ttgccagagg cgcaggagta cagcacgtaa tgagcgccga
     5761 cattgcgggc aatgcctatg gctttactac gggtgcctaa actgtcctgc ggcgacaaac
     5821 ctaactgttg cttagccatc gacagttgct gggcggaaac cagggtaaat ttcccgttat
     5881 tagccagcgc atttcgcagc gtttcggtcg cttctgcggc attcagcgaa ccgttagtac
     5941 ggttgttaac gctatcaacc agcaggacgc tacccgcagt caccccgtca gccccaagca
     6001 tcttactgac catcggctgc attgcgccat tccagtcata atggcgaata tgcggcgcag
     6061 gcggtgccgt tcgatcttcg tgctcaattg ggcctggctg ctgcgggatc gtcggcaccg
     6121 agggcactgt ggggacaggc tgttgtggct cggctggttg ttccggcgct ggtttcactt
     6181 cttctaccgg tgcaggttca cgttgcccca cacacccggc gagaaacatc gccagcgcgg
     6241 taatcaaggc gtagcgactc atttttgtca tcaagattca cccctttaca aatatagata
     6301 aagtctgact ttgtgcgccc ccagaaaatt ggcgctgccg tacagcgtta ccgccgaatg
     6361 tgcgggaatg gtaacgctgc gtggcctttc cagaggatgc atctccagcc ctctggcgtc
     6421 ataccagtaa aaacgataat gaacggtaac gggttcttgc ctttcgttat aaatcgttga
     6481 ggatgctgaa ggttgaatat cagacgtcga aaggacgggc ttttctgcac tgatgcccgc
     6541 agccaataaa gatgactcca tcaccagcga ttgctcatca ttcaccggaa tttccggatg
     6601 tgaacgacag cccaccagca gtaacaacac cagagacacc agcccaaagc atccttttct
     6661 catcgttaca gacctttatg cgccagcatt ggtcccagcg gacggccacc caacaagtgc
     6721 atatggatgt ggtaaacctc ttgtccgcca tggcggttgg tattcatgat cagacgatag
     6781 ccatcttcgg caataccttc ttgctcagca atttttgccg ctacggtgat catgcgtccc
     6841 agcgcctgct catgctcagc tgacacgtcg ttcacagtcg gaatgagaat attcggaatg
     6901 atcagaatat gcgttggcgc ttgcggcgaa atatcgcgaa acgccgttac cagatcatcc
     6961 tggtagacga tatcggaggg gatctcacga cgaataattt tgctgaatat agtttcttct
     7021 gccacgacat tttccttttt caaatagcca tgcgttgcgc catgctacgc cgcgccacac
     7081 tgcgagtata gagtatggtc gagttaaacc tgctctttca atcttaactc acgatttctt
     7141 aagcaaaaaa tcgttttgct gccgtatata tcgccattat tcccatttct gcctgcgaat
     7201 gcgctgaaaa gacattctct gtttggttta cacgacaaat atgaatgcgt atatttctca
     7261 tttgcatttg cagcaaaatt attcgcacac taaatataac cgctcatcac cctcctccgg
     7321 catgagcctg acgacaacat aaaccaagag atttcaaatg ctttcaacac aatttaacag
     7381 ggataatcaa tatcaagtca tcaccaaacc gtcactactt gccggttgca tagcactggc
     7441 actattacct tctgccgctt ttgctgcacc agccactgaa gaaacggtga ttgttgaggg
     7501 ttcagccaca gctccagatg atggcgaaaa tgattacagc gtaacgtcta cctctgcggg
     7561 taccaaaatg cagatgactc aacatgatat tcctcagtcg gtcactattg ttagccagca
     7621 gcggatggaa gatcagcagt tacaaacgct gggcgaagtg atggaaaaca cgctggggat
     7681 cagcaaaagt caggcggatt ccgatcgtgc tctttattat tcccgcggat tccagatcga
     7741 taactatatg gttgatggta tccccaccta ttttgaatcg cgctggaatc tgggcgacgc
     7801 actttctgat atggcactgt ttgaacgcgt agaagtagtg cgtggcgcga caggactcat
     7861 gaccgggacg ggtaatccat ctgcggcaat taatatggtt cgaaaacacg cgacctgtcg
     7921 tgaatttaaa ggcgatgtct cggcggaata cggtagctgg aacaaagaac ggtatgtggc
     7981 ggatttacaa agcccactca ccgaagacgg taaaatccgc gcgcgaattg tcggcggcta
     8041 ccagaataac gactcatggc tggaccgcta caatagtgaa aagaccttct tctcgggcat
     8101 tgtcgatgct gatttaggcg atcttacgac gctttcagcc ggttacgaat atcagcgcat
     8161 tgatgttaat agcccaacct ggggcggttt accgcgctgg aatactgatg gcagcagcaa
     8221 cagttacgat cgcgcacgca gtaccgcacc tgactgggcg tacaacgata aagagatcaa
     8281 caaggtcttt atgaccctga agcagcggtt tgctgatacc tggcaagcga cactgaatgc
     8341 cacccactct gaagtcgaat ttgacagcaa aatgatgtat gtcgatgcct atgtaaacaa
     8401 agcggatggt atgctggttg ggccatacag taattatgga cctggctttg atcatgtcgg
     8461 cggcaccggt tggaacagtg gcaaacgtaa agttgatgcg ctggatttgt tcgctgacgg
     8521 tagttatgaa ttgtttggtc gtcagcacaa tctaatgttt ggtggcagtt acagcaaaca
     8581 aaacaatcgt tcagttcatg ggccaacatc ttcccggatg aaattggcag tttctacaac
     8641 tttaatggca atttcccaca agccgactgg tcatcacaga gcctggcgca ggacgatacc
     8701 acacatatga aatcgttata tgctgccact cgtgtcaccc ttgccgatcc gctgcatctg
     8761 atcctcggcg cacgttatac caactggcgg gttgatacgc tgacttacag catggagaaa
     8821 aaccacacca cgccttacgc tggtctggtg tttgacatca atgacaactg gtcgacctac
     8881 gccagctata cctctatttt ccagccgcaa aatgatcgtg acagttcagg caaatatctg
     8941 gctccaatca ccggtaacaa ctacgagctg ggtctgaaat cggactggat gaatagccgt
     9001 ctgaccacca cgttagccat cttccgtatt gagcaggata atgtcgctca gtccaccggt
     9061 acacctatcc ccggcagcaa cggcgaaacc gcctataaag cggtggatgg gacagtcagt
     9121 aaaggggtgg aatttgaact caacggcgca attaccgaca actggcagct gacatttggc
     9181 gcaacgcgct atcttgcaga ggataacgaa ggaaacgccg ttaatcctaa tctgccacgc
     9241 accacggtta aaatgttcac cagctatcgg ttgcctgtca tgccagagtt gacagtcggc
     9301 ggtggtgtta actggcaaaa tcgcgtgtat accgacaccg tgacaccgta tggcaccttc
     9361 cgcgccgagc aaggtagcta cgcgctggtg gatctcttca cccgctacca ggtgacgaaa
     9421 aacttctcgt tacaggggaa cgtcaataac ctgttcgaca aaacctacga taccaacatg
     9481 gaaggttcta tcgtctacgg cgcaccgcgt aatttcagca ttaccggcac gtatcaattc
     9541 tgaataacac ctgtaaaaaa ggcagccatc tggctgcctt agtctcccca acgtcttacg
     9601 gattagtggt tacggatgta ctcatccatc tcggttttca ggttatcgga tttagtaccg
     9661 aaaatagcct gaacaccaga accagcaacc actacgcccg ctgcgcccag tttcttcagg
     9721 ccagcctgat ccactttaga cacatcagca acgctgacgc gcagacgggt aatacatgcg
     9781 tcgaggttag taatgttttc tttaccacca aatgcagcaa ccagagccgg tgccatttcg
     9841 ctggtacctg ttgcttttgc atcttcagtc gcgtcttcac gacccggcgt tttcagatcc
     9901 agtgctttaa tcagcacgcg gaagatggtg tagtaaacaa tcgcataacc gataccgacg
     9961 atcggtaaca gccacagttt gctgctgtta ccagacagaa cgatgaagtc gatcagaccg
    10021 tgcgagaacg acgtaccgtc acgcatcccc agaagaatac agattgggaa tgccaggcct
    10081 gccagaatcg cgtggatgat gtacaggatc ggcgcaacga acatgaagaa gaactcgatc
    10141 ggctcggtga taccggtcag gaacgaggtc agcgccgcgg agatcataat accgcccact
    10201 ttcgcgcggt tttctggttt agcagagtgc cagatagcaa ttgcggcagc tggcagaccg
    10261 tacattttga acaggaagcc accagacagt ttacccgcag tcgggtcacc cgccatataa
    10321 cgcggaatgt cgccgtggaa aacctgacct gctgcgttgg tgtattcacc aatctgcatc
    10381 tggaaaggta cgttccagat gtggtgcaga ccaaacggta ccaggcaacg ttcgatgaaa
    10441 ccgtaaatgc caaacgcaac taccgggttc tggtaagcag cccactgaga gaaggtctgg
    10501 attgcagaac caatcggcgg ccaaatgaag gacagcacaa cgccagtaaa gatggcagcc
    10561 aggccagaaa tgatcggcac aaagcgttta ccggcaaaga agccaagata ctcaggcagc
    10621 ttaatacggt agaaacggtt aaacatgtac gctgcaatcg caccggagat aatccctccg
    10681 agtacgccag tatccgccaa gtgtttagag gcgatttctt cagcaggtaa atgcagtacc
    10741 agtggcgcaa ccacggccat ggttttaacc atgatgccat aggcaacaac tgcggccagc
    10801 gcggatacgc catcgttatt ggtaaagccg agggcgacac cgatcgcaaa aatcagtggc
    10861 atgtttgcaa agacggaacc gcctgcttct gccataacat gcgatacaac ggcgggcagc
    10921 cagctgaaat tcgcggaacc gacgcccagc agaatacctg cgataggtag tacggatacc
    10981 ggcagcatca gcgatttacc gaccttttgc aggttagcaa atgcattctt aaacataatt
    11041 gagagtgctc ctgagtatgg gtgctttttt tacgttctca cgcgtggcaa ggggggagag
    11101 cctcgccgtg tacagggcat ctaagcgccc tttatttatt acacagagta aaataattca
    11161 gtgccaattt gtttgacggc tatcacgttt caacttcaga tcattggaga agttgcacat
    11221 tttgacaaaa catatgtcac aatatttcaa acgcggttat tcaccgccca attttcggcg
    11281 gtgaacttta ctcgttttag ccattaatta cgagctttaa aaaaactcat tcaacggatg
    11341 gattgaaggc gggaagcgtc gatgtgaaac agacgggcga agttatcggt ggttacctgc
    11401 gccagttctt caacggcaac acctttcaac acagccatgt attctgcaac gtcacgaacc
    11461 atcgcaggtt gattctcttt tcctcgatgc ggtaccggcg caaggtaagg tgagtcagtt
    11521 tccaccagta atcgatccag ggggacataa cgcgcagcat cgcgcagttg ctccgcatta
    11581 cggaaggtca caatgccgga aaaggagatg taaaatccga gatccagtaa tttacccgcc
    11641 gtttctctgt cctctgtaaa acagtgtagt acgccaccgc aatccgtcac tttttcttcg
    11701 cgcagaattg ccagcgtatc ggcacgggcg tcacgggtat ggacgataac cggcttgttc
    11761 agttcacgac caatctggat atgatggatg aaggactctt gctgacgtac tttagtttcc
    11821 ggtgtgtaat aataatccag cccggtttca cccagcgcta caacaccctc ttctgccgcc
    11881 agacggcgta aatcttctac atcgtaggga tcattctggt tgagtggatg cacgccacaa
    11941 gaaaatacga cgttgtcacg ttcgcctacc agatcccgca tatgtaaata acccggtaat
    12001 gttgtggcga ctgccagaca aaatttcaca tcgcgtgcgg cggctttcgc cagaacgtca
    12061 tccacgtcct tatgcaaaga ttcataatcc agaccatcga gatggcagtg tgagtcgact
    12121 aaaaacatga tgtctctctt aaagatgagg aaccggtaac acaacgcccg gttgcaggta
    12181 atgctcaata cgcagtaaaa gatcggtgat gagaagctcg cggttgatgc ctgtgacaga
    12241 cattaactgt tcacgaatgt ggcaaacatc ccccagtata gcctgcaggc acgagggaga
    12301 aagatggttt gccagttcgg cgaccaggcc cggcacatca acattggtca cctgcgcagc
    12361 gccatgatgg cgttttagcg catccatcag caacgttgcc agccagtgta aacgcgccgg
    12421 agcttgttca tgattaaggg ccgccagcag cgaataccag tcgcccgatg gcacgctata
    12481 tgccaacgcc tgacacaatg tttcacgagc ctgccagtta tctccctgaa acaacgccag
    12541 tgccgcgcca ggcgaaccgg cgcttaagcg caatgcggca agtaatgcat cctgtgacat
    12601 tgtcacttcg cgtgaaagcc aagtcacggc gtactgttct ggaggcggcg caaggtaatg
    12661 taaccgacaa cgactacgta atgttgccag taaacgttca ggctcgcggg tagccaggaa
    12721 aaaccaggtt tctgctggtg gctcttcaag cgttttcagc aatgcgttag ccgcggcgtc
    12781 ggtcagtaag gcagcatcgg ttacccagac aactttcgca ccacctaggc gtgcgtgctc
    12841 attcagcttt tcggtgacct cacgtaccgc atcaatgccc agcgtatttt tccctttttc
    12901 gggagccagg gtgtagtaat cgggatgcgt gccagcctgc atcaactgac atccacgaca
    12961 gtgaccgcaa cttttgtggc cctgcggttg ttggcagagt aaataacggc tcagggcgta
    13021 gattaaagca tcatcgccca tgcccggtaa cgcctgaatg agtagcgcat ggtgacctct
    13081 tccggcctga tagctggcta ccagtttttc gaaatcaggt cgtaaccatg gataccatct
    13141 catgcgtcca actccttcac ccagtgggtc acggtagtgc ggattgcatc catcacggcc
    13201 tccagcggct gggtggcatc aatggtatga atacttttat cttgtgcagc cagttccaga
    13261 tagcgggcgc gggtgcgatt aaagaaatcg aaagactctt gctcaatacg atccagctcg
    13321 ccgcgcgcac gcgcgcgttt taagccaact tccggggtaa catcgagata gagcgttaag
    13381 tctgggcgaa aatccccgag aacagcatca cgcagtgttg ccagcatatg ttggtcaata
    13441 ccacgtcctc cgccctgata cgcctgagtg gaaagatcgt ggcgatcgcc aatcacccag
    13501 gtgccgttag ccagcgcagg tttgatgacc gtttctacca gttgaacgcg cgcggcataa
    13561 aacatcagaa cttcggcttt atcggtaatg acttcatcgc ctaccgattt gatatccagc
    13621 accaggcttc ttaacttttc ggcaagttgc gtaccgccag gttcccgagt gaaaaccatg
    13681 tcgcggatac ccagttgctc gagcgtctca accaccacat tacgcgcggt agttttgcct
    13741 gcgccttcca gcccctcaat gacgatatac ttactgcgca tttttttcct taagcacttt
    13801 cagataatcc tgcacagact tgttatgact ggcaagattg gtattaaacg tgtgaccacc
    13861 tttaccatcg gccacaaaat agagatacgg cgtttttgcc ggatgcgcag cagccttcag
    13921 cgaatccgcc cccggcgtag ctatcgcacc cggcggcaaa ccggtaatgg tataggtgtt
    13981 atacgctgtc ggcgtttcca ggtctgcacg agaaagtttg ccattataac gctctcccat
    14041 cccgtaaatc acggttgggt cggtctgcag gcgcatacca atgcgtaaac ggttgataaa
    14101 taccgaggca acctgatcgc gttcactggc aacggcggtt tctttttcga taattgatgc
    14161 catcgtcacc agctgatttt tatctttata aggcagaccg tccgcacgcc cttcccaggc
    14221 gctatcgacc gctttcacca ttttcttgtg aactcgcttg agtaacgcga catcggtggt
    14281 attggcggta tacatcaagg tgtctggcca gaaccaacct tcaatccact ccgggttttc
    14341 cagttcaagt gcctgcgcta cggtggcgta cttatcgtca ctcagcgtgt gcttgatata
    14401 cggagcctca cgcaattgct tgaggtagtc gctcagacgc atcccttcta ccagtcgcag
    14461 cgggaactgt gcttctttac cgctttccaa caatttcagc atctcgcgca cggtcatctg
    14521 cggtgtaaag cggtaagtcc cggctttaaa gtgagaaaga tccggttcga tacgcagcag
    14581 ccattgaaaa acccgtgggc gattgatgat cttatcagca taaagctgtt caccgagcgc
    14641 cagacgtccg gtccctggct tcagggtaaa tatcgtctct tctttgataa gcaatttgct
    14701 gtcggcaaga tggcgaacct tccagacgcc cacaccagcg gcgataccca gtaccaccag
    14761 caataacaag ataatcaata acactttttt catgactaat tcgggcgctc acaaagtggg
    14821 gctaaatatt catataacgt tgctgacgaa aaggtgacat cgccacaggc acgtacgggc
    14881 atcactggca ttaacgcatt acaaataacc atctcatctg cctgcaacgc ctcctccagc
    14941 gaggcttgca cttcgacaag ctgataagag gattgtgcca gtaaacggat acagaattgt
    15001 cgcataatgc cgttaacacc tgcctgatcc agtcgcggcg tataaactac attgcccttc
    15061 cgccagaaca aattagccgc acagcattcc gtaacccacc cttcgctgtc aaggaccagc
    15121 gcctcatcag cgtttgtctg ctcaagatga gagcgaatca atacttgctc aagccgatta
    15181 agatgtttaa tacctgcaag atgaggattg cgccccagcc gcaccgggct tagcgccaac
    15241 gtcatccctt cgttacgcaa acggtcgtaa tgtgcaggat aagccgtaac ggagagaatc
    15301 cgcgttgctg gtccgctgtt caatgtgctg taccctcgcc cgccactacc gcgactgatc
    15361 acgactttca gcacaccatt ttgctgttct gctgccagcg ttttcatctc ttgttcaagc
    15421 tgaggccaga agtcacagga aatcatcaac cgctgacaag catcctgtag tcgcaggata
    15481 tgcgccgata acaaactgac tttaccgtcg ataactctgg cggtggtaaa acaaccatca
    15541 ccaaactgcg ttgcccgatc gcttactgcc agcgattcct gcttataacc gttaattaag
    15601 aacataccgg ctccttatca gagaattgtt agtgtggcag catgttcact acggaacaag
    15661 tcggaataaa agctaagaaa aaaggcccgc aagcggacct tttatatggg tggaaaatga
    15721 caacttagat ctttttaaag atcaaagaac cattagtgcc accgaagccg aaggagttac
    15781 acagcgtgta ttccattccg ctgacctgac gtgcttcatg cggtacgaaa tccagatcgc
    15841 aaccttcatc cgggttatcc aggttgatgg ttggcggaac agcctgatcg cgcagcgcca
    15901 ggatggagta gatagattct actgcacccg ccgcacctaa caggtgaccg gtcatagatt
    15961 tcgtggagct taccaacaca cggcttgcag cttcaccgaa gatggttttc accgcctgcg
    16021 cttcagcttt atcgccagcc ggcgtagaag taccgtgcgc gttaacgtag ccaatctgac
    16081 tcgcttcaat gcctgcatca cgcagagcat ttgccattgc cagagctgcg cctgcgccat
    16141 tttctggcgg tgacgtcata tgataagcat cgctgctcat accaaagccg acgagttcag
    16201 cgtaaatttt cgcaccgcgt tttttcgcgt gttcgtactc ttcaagtacc agcataccgg
    16261 caccatcgcc cagtacgaag ccatcacgct ctttatccca cgggcggctc gccgcttgcg
    16321 ggttatcatt gcgggtagat aatgcacgtg ccgcgccaaa accaccaacg cccagcggcg
    16381 tactggcttt ctctgcgcca cctgcaacca tcacgtcagc atcgccatac gcgataatac
    16441 gcgcagcatg gccaatgttg tgcacgccgg aagtacaggc ggtcgcgata gagatgcttg
    16501 ggccacgcag gccatacatg atagtcagat gacctgccac catgttcaca atcgttgacg
    16561 gaacgaagaa tgggctgatc ttacgtggac caccgttcat cagagatgtg tggttttctt
    16621 cgatcagtcc gaggccgcca atcccggagc caattgcggc accaatgcgg gttgcgttct
    16681 cttccgttat ttcaaggcca gaatcctgca tggcctgaac gccagcgaca attccatatt
    16741 gaatgaaggc atccatcttg cgctgttctt tgcgcgagat aatgtcctca cagttaaaat
    16801 cctttactaa gccagcaaat ttcgttgcat aggcgctagt atcgaaatgg tcgattaggc
    16861 tgatgccact ctgaccggca agcagagctt tccaggtaga ctctacggta ttgccgacag
    16921 gagacaacat gcccagtccg gtcacaacta cacgacgctt agacacgttt gtcctccaag
    16981 gagggaaaaa tgattctagt gggacaaaaa gataaaactc aggcggtcga acgaccgcct
    17041 ggagatgttc acttacgcct ggtggccgtt gatgtaatca atggcagcct gaacggtggt
    17101 gattttctca gcttcttcgt ccggaatctc agtatcaaac tcttcttcca gagccattac
    17161 cagctcaacg gtgtcaagag aatccgcgcc caggtcttca acgaaagaag cattgttggt
    17221 aacttcttcc tgcttaacgc ccagctgttc gccgataatt ttcttaacgc gttcttcgat
    17281 agtgctcata ctcttaaatt tcctatcaaa actcgctttc gcgatggttt tcgtagtgta
    17341 taaaatgttg aaaaatttgc aactaaatcc cggcaggtct taccacgatt ttacgttatt
    17401 ttgaggcctt accccccaat aacgcaaatc attttgcact aattattact cataaccacg
    17461 cagactgcgc gcaacatgag cattttgtgc aaatcgcggt cagaccatgt acatcccgcc
    17521 gttcacatgc aaagtttcac ccgtgatgta agctgcttcg tcggatgcca ggaatgcaac
    17581 cgcgttggcg atttcctgtg cgccgccgag gcgacccgca ggaacctgcg ccaggatacc
    17641 cgcacgctgg tcatcgctca gcgcacgtgt catgtccgtt tcaataaagc ccggagcaac
    17701 aacgtttaca gtaataccgc gtgacgcaac ttcgcgcgcc agtgatttac tgaagccgat
    17761 caagcccgct ttcgccgcag cgtagttggc ctgaccgcca tttcccatgg taccaaccac
    17821 agaaccgata gtgataatac gaccatgacg ctttttcatc atagcgcgca ttaccgcttt
    17881 tgacagacgg aaaacagatg aaaggttagt ttcgataata tcgttccact cttcatcttt
    17941 cattcgcatt aacaggttat cacgagtgat accggcatta ttgaccagga tatccacttc
    18001 accaaattct gcgcgaattt tttccagaac agattcgata gatgccgggt cggtcacatt
    18061 caacatcaga cctttgccgt tggcacctaa ataatcactg atcgcctgag cgccattttc
    18121 actggtcgca gtgccaataa ctttcgcgcc acgggctgcg agcgtttcag caattgcgcg
    18181 gccaattccg cggcttgcac cggttaccag tgcgattttt ccttcaaaat tcatgatttt
    18241 cctcttttaa agctcgagcg ccgctgccat cgctgaaggt tcgttcagcg ccgaggcggt
    18301 cagggtgtcg acaatgcgtt tcgtcaggcc agtaagcacc ttgcccgggc cgacttcata
    18361 gagatgttct acgccttgcg ctgccatgta ctcaacagac ttcgtccact gaaccgggtt
    18421 atacaactga cgtaccagtg cgtcacggat ggcatcacca ttggtttcgc atttcacatc
    18481 aacgttattc acaacagaaa ctgttggtgc gttaaaggtg attttcgcta attctactgc
    18541 cagtttgtcg gctgctggtt tcatcagcgc acagtgagac ggtacgctca ctggtaacgg
    18601 cagcgcgcgt tttgcgccag cagctttaca ggcagcgcca gcacgctcaa ccgcttcttt
    18661 atgaccggca ataaccacct gtcccggaga gttaaagttt accggagaaa cgacctgacc
    18721 ttctgcagct tcttcacacg ctttcgcaat aggcgcatca tccagaccga tgattgccgc
    18781 catagcgccc gtgccttccg gtacggcttc ttgcatgaac ttaccgcgca tctcaaccag
    18841 acgcaccgca tcagcgaaat caatcacgcc agcgcaaacc agcgcggagt attcccccag
    18901 gctgtgaccg gccatcattg ccggtgcttt accgccctgc tgctgccata cgcgatacag
    18961 cgcaacagat gcagtcaaca gcgctggctg ggtttgccag gttttattca gttcttcagc
    19021 tgacccctgc tgggtcagcg cccacaggtc gtagcccagc gccgcagaag cttcagcaaa
    19081 cgtttcttcg acaattggat agctcgccgc catatcagcc agcattccaa cggtttgaga
    19141 accctgtcca gggaacacaa atgcaaattg cgtcatgttt taatccttat cctagaaacg
    19201 aaccagcgca gagccccaag tgaatccacc gccaaaggct tcaagcagaa ccaactgccc
    19261 cggcttaatg cgcccgtcgc gtacagcttc atccagcgcg cacgggacag aggccgcaga
    19321 ggtattaccg tggcgatcca gcgtcaccac gacattgtcc atcgacatac cgagtttttt
    19381 cgccgttgca ctgataatac gcaggttagc ctgatgcgga accagccagt ccagttgaga
    19441 acggtcaaga ttattcgccg ccagcgtctc atcaacgatg tgcgccagtt ccgttaccgc
    19501 aaccttgaag acttcgttgc ccgccatcgt cagatgaatt gaattctctg gattcacgcg
    19561 gtcggcgttt ggcagcgtca gcaattcacc ataactaccg tcggcatgca gatgggtgga
    19621 aatgattccc ggctcttcag aggcagccag caccgcagcg cccgcgccat cgccaaaaat
    19681 aataatagtc ccacgatctg ttggatcgca ggtgcgcgcc agtacatcgg aaccgacgac
    19741 cagagcatac ttcaccgccc cagatttcac gtattgatcg gctacgctta atgcataggt
    19801 gaaacctgca caggctgctg caacgtcaaa tgccgggcaa cctttaatgt ccaacatgct
    19861 ttgaatctga caagctgcgc tcgggaaagc gtgcgtagca gaagtcgttg ccacaacgat
    19921 caggccaatc tggtctttct caatgcccgc catctcaatt gcgcgtgtcg ccgcttcaaa
    19981 gcccatggtt gaaacggttt cgtttggcgc ggcaatgtgg cgttcgcgga taccggtacg
    20041 agtgacaatc cactcgtcag aggtgtccac cattttttcc aaatcggcgt ttgtccgcac
    20101 ttgttcgggc agatagctgc cagtaccaat aatcttcgta tacatgtacg ctcagtcact
    20161 tttcggttat ataccgtcac ttgcaaactg cgagttcgct ggcagcgtcc tgctaccgca
    20221 gagttccgct tttgccaccg tccagcagct caaaaccagc tgggtataca gattccaggc
    20281 gagcggcaat tcgctgagga acttgtcgct gcaccgcctg cactgcctgt tcaatcgcga
    20341 ccgcaaaagc tcgctgattg gctgcaccat gactttttat caccgtgccg cgcaatccta
    20401 acagacaggc gccgttatac tggtcggggt tgaggtgact gaatcgcctc gtcaggctct
    20461 tttgtagcca acgctttaat aacagtagcc accacgaccg ttttttccct tcaccctgag
    20521 atttcagcag agaaaggaac atcctgacaa caccttccat cgtctttaac gtgacatttc
    20581 ctgtaaagcc gtcacaaacc agcacatctg tcttgccagt taacaactca ttggcttcaa
    20641 gatagccgat ataattgata gaagggattg ttttaagcac cgctgaggca tcccgaatac
    20701 tgtcaagacc ctttacttct tcttcaccaa tattgagcaa cgccacgcga ggattgggaa
    20761 tttccaccac ctcttcagcc agaactgagc ccataatggc aaattgcacc aacattgtgc
    20821 tgtcacaatc gacgttggcc cctaaatcga ggaccaccgt tttgcccttt tgctgatgtg
    20881 gtaataccgt caccagcgcc ggacgctcaa tcccctccag gggcttgagt aataattttg
    20941 ccagccccat cagtgccccg gtattaccgg cactgacaca ggcttgcgct cgaccttctt
    21001 tcaccagctc cagggccata cgcattgaac tcccacggct ggcgcggata gcttgcgaag
    21061 gccgggcatc actggcgata actgactgcg caggaataat ctgcagacgc gaacgttgtt
    21121 caaagtcagc tttagcaagt aatggcgtga tggcgtcggg attgccgact aaaagaagag
    21181 tgagttgcga attagaattc agtgcctgca atgctgcagg cactgtcacg gaagggccaa
    21241 aatcccctcc catgacatct aacgccaggg ttagacgtgt caaggtatcg tcgccgcccg
    21301 gtttggtctt tccccagttg cctggggaaa atcctcacta agcttcatca cgcagacgcg
    21361 tgattactta gcgatgacct tgcggccgcg gtagtaaccg tcggcagtga tgtggtgacg
    21421 caggtgtttt tcaccagaag ttttgtctac agacaggctg gtgactgcgg tcagcgcgtc
    21481 atgggaacga cgcatgccac gtttggaacg ggttggttta ttctgttgta cggccatgga
    21541 ccttactcct caattacggt ttatccccga tccaacgggg agcaccaatt acttacgctt
    21601 taagctggct aatacggcaa atgggtttgg cttttgcact tcttcaggca gttcaccaaa
    21661 gaccatgtcc gcttcggaca cttcacagtg ttcagaatca tgcaccggaa ctaccggcaa
    21721 ggcgaggatg atttcatctt caaccattgc aagcagatcg atttcaccga attcgttaac
    21781 ctcaatcggt tcatacgctt ccggcagtgc ttcagcctgt tcgtctgaac gcacaggact
    21841 aaaacaatac gttgtgtaga cctgatgagt aaacggcttc ccgcaacgct gacactcgag
    21901 cgttaccgtc accttcgcat cgccgtttaa cactgcgaga cgttggttat cgatagcgaa
    21961 cgacatggag cattccacat cactgtccac actgactacg gattcggcga cgcgctcaac
    22021 ctgatcaggg gtatagatac cctggtaatc aaggcgtttt tgagccgtac gaaccggatc
    22081 gagagtcagg ggtaatttta ccttttgcat agggcgcgca tattaacttt gtaacgtcat
    22141 agagtcaaag aaaaaggcag cctgtggttg ccttttgcca ataattcgca cacattgcgg
    22201 gttactgctt tattttcgtt cagtgctgat aacgaacatc aacctgcaaa acgaagcgca
    22261 tagtttaaaa tggctatcat caatacgcta tatctgatgg aaaaaaatat gcctaaactt
    22321 attttagcct ccacctcgcc atggcgtcgc gctcttctgg aaaaactgca aatctctttt
    22381 gaatgtgcag cacctgaggt cgacgaaacc ccacgcagcg acgaatcacc gcgacagttg
    22441 gtgcttcgac tggcacaaga aaaatcgcaa tctctggcgt cacgttatcc ggatcattta
    22501 attattggtt cagaccaggt atgtgttctt gatggcgaaa tcaccggtaa accgttaacg
    22561 gaagaaaatg cccgtctgca attacgcaaa gccagcggca atatcgtcac cttctatact
    22621 ggactggcgt tgtttaattc ggcgaatggg catctacaaa cagaagtgga gccttttgac
    22681 gtccatttcc gtcacctaag cgaggcggag attgataatt acgtgcgtaa ggagcatccc
    22741 ctgcactgcg cgggtagctt taagagtgaa ggatttggca ttacgctgtt tgagcgttta
    22801 gagggacgtg accctaacac gctggttggt ttaccgctaa tcgcgctgtg tcagatgtta
    22861 cgtcgggaag ggaaaaaccc gctgatggga tagttgtttg cgccggatgc ggttacgtcg
    22921 catccggcaa tcggtgcagg cggcctgatg cggcgtaaac gccttatcag gccaaccgcc
    22981 cattatcagg cttatatgct tgtattagcg cgcgttacgc agcttttgca aacaacgctt
    23041 caaaccttca tccatcggcg cttcgatacg catcacctca ccggtccccg gatgggtaaa
    23101 cttcaacgct gcggcgtgca ggaacagacg atttaatccc gtgcctgctt cagtgagctg
    23161 tctgtcaaat tcacggtcac cgtagcgatc gtcaaaggca atcggatgac ccgcatactg
    23221 tgtatgcaca cggatctgat gagtacgtcc ggttaccggg ctacaacgca ccagggtggc
    23281 aaatgcatag cgttcttcca ctttaaagcg tgtttccgac ggtttgcctt cctgactgac
    23341 acgcacgata cgttcgccgc tttgcagaat atttttcaat aacggcgctt gaacgctctt
    23401 cacatgcgac tgccactgac cgcgcaccag cgccaggtaa tctttttgca ttcctttttc
    23461 acgtaattgc tcatgcagag aacgcaacgc cgagcgtttt ttcgctacca gcaacacacc
    23521 tgaagtgtcc cggtcgagac gatgaaccag ttcaaggaac cgcgcttccg ggcgcaacgc
    23581 ccgcaaacct tcgataacgc cgaagcttaa accactgccg ccatgtaccg ccgtaccgga
    23641 aggtttattc agcaccagga tatgatcatc ttcatataag atgacgtccg ccagcgccgc
    23701 caccttttgc agatgtggcg aaaccgcctc ttcttcccgc tcagcaacgc gaaccggtgg
    23761 gatacgcacc tcatcacccg cttcgagttt atattcaggc ttaatacgtt ttttgttcac
    23821 ccgcacttca cctttacgca aaatacggta aatcatactt tttggtacgc ctttcaattg
    23881 ggtacgcaaa aagttatcga tacgttgccc cgcttcgtca gcggtgatag caacaatttt
    23941 tacggatgga gtctctgttt tcatggtggg cgattctaat tagccaacag gattcgcgcc
    24001 actcattttt ctatgcttat ttttagtttt gcaccttatt acttcactac gtgatcactt
    24061 tattgatggt tattaaacca atcaccagca agaagtgaaa aaactgtgag taagcgggtg
    24121 ataaatggta aaagtcatct tgctataaca aggcttgcag tggaataatg aggccgtttc
    24181 cgtgtccatc cttgttaaaa caagaaattt tacggaataa cccattttgc ccgaccgatc
    24241 atccacgcag caatggcgta agacgtattg atctttcagg cagttagcgg gctgcgggtt
    24301 gcagtcctta ccggtagatg gaaatatttc tggagagtaa tacccagtct gtttctttga
    24361 taattgcgct gtttttccgc atgaaaaacg ggcaaccgac actctgcgcc tctttgagct
    24421 gacgataacc gtgaggttgg cgacgcgact agacacgagg ccatcggttc acacccggaa
    24481 aggcgttact ttgcccgcag cttagtcgtc aatgtaagaa taatgagtaa gttacgatga
    24541 aaagaatgtt aatcaacgca actcagcagg aagagttgcg cgttgccctt gtagatgggc
    24601 agcgtctgta tgacctggat atcgaaagtc cagggcacga gcagaaaaag gcaaacatct
    24661 acaaaggtaa aatcacccgc attgaaccga gtctggaagc tgcttttgtt gattacggcg
    24721 ctgaacgtca cggtttcctc ccactaaaag aaattgcccg cgaatatttc cctgctaact
    24781 acagtgctca tggtcgtccc aacattaaag atgtgttgcg tgaaggtcag gaagtcattg
    24841 ttcagatcga taaagaagag cgcggcaaca aaggcgcggc attaaccacc tttatcagtc
    24901 tggcgggtag ctatctggtt ctgatgccaa ataacccgcg cgcaggtggc atttctcgcc
    24961 gtatcgaagg cgatgaccgt accgaattaa aagaagcact ggcaagcctt gaactgccgg
    25021 aaggcatggg gcttatcgtg cgcaccgctg gcgtaggcaa atctgctgag gcgctgcaat
    25081 gggatttaag cttccgcctg aaacactggg aagccatcaa aaaagccgct gaaagccgcc
    25141 cggctccgtt cctgattcat caggagagca acgtaatcgt tcgcgccttc cgcgattatt
    25201 tacgtcagga tatcggcgaa atccttatcg ataacccgaa agtcctcgaa ctggcacgtc
    25261 agcatatcgc tgcattaggt cgcccggatt tcagcagcaa aatcaaactg tacaccggcg
    25321 agatcccgct gttcagccac taccagatcg agtcgcagat cgagtccgcc ttccagcgtg
    25381 aagtccgtct gccatccggt ggttccattg ttatcgacag caccgaagcg ttaacagcca
    25441 tcgacatcaa ctccgcacgc gcgacccgcg gcggcgatat cgaagaaact gcgtttaaca
    25501 ccaacctcga agccgccgat gagattgctc gtcagctgcg cctgcgtgac ctcggcggcc
    25561 tgattgttat cgacttcatc gacatgacgc cagtacgcca ccagcgtgcg gtagaaaacc
    25621 gtctgcgtga agcggtgcgt caggaccgtg cgcgtattca aatcagccat atttctcgct
    25681 ttggcctgct ggaaatgtcc cgccagcgcc tgagcccatc actgggtgaa tccagccatc
    25741 acgtctgccc gcgctgctcc ggtaccggta ccgtgcgtga caacgaatcg ctgtcgctct
    25801 ctattctgcg tttgatcgaa gaagaagcgc tgaaagagaa cacccaggaa gttcacgcca
    25861 ttgttcctgt gccaatcgct tcttatctgc tgaatgaaaa acgttctgcg gtgaatgcga
    25921 ttgaaacgcg tcaggacggt gttcgctgcg tgattgtgcc aaacgatcag atggaaaccc
    25981 cgcactacca cgtgttgcgc gtgcgcaaag gggaagaaac gccgacctta agctacatgc
    26041 tgccgaagct gcatgaagaa gcgatggcgc tgccgtctga agaagagttc gctgaacgta
    26101 agcgtcctga acaacctgca ctggcaacct ttgccatgcc ggatgtaccg ccagcgccaa
    26161 ccccagctga acctgccgcg actgtcgtag ccccagcacc gaaagctgca acggcaacac
    26221 cagcagctcc tgcacaacct gggctgttaa gccgcttctt cggcgcactg aaagcgctgt
    26281 tcagcggtgg tgaagaagcc aaaccgaccg agcaaccaac accgaaagca gaagcgaaac
    26341 cggaacgtca acaggatcgt cgcaaacctc gtcagagcaa ccgccgtgac cgtaatgagc
    26401 gccgcgacac ccgtagtgaa cgtactgaag gcagcgataa tcgcgaagaa aaccgtcgta
    26461 atcgtcgcca ggcacagcag caaactgccg agacgcgtga gagccgtcag caggctgagg
    26521 taacggaaaa agcacgtacc accgacgagc agcaagtgcc gcgtcgtgaa cgtagccgcc
    26581 gccgtaacga tgataaacgt caggcgcaac aagaagcgaa ggcgctgaat gttgaagagc
    26641 aatctgttca ggaaaccgaa caggaagaac gtgtacgtcc ggttcagccg cgtcgtaaac
    26701 agcgtcagct caatcagaaa gtgcgttacg agcaaagcgt agccgaagaa gcggtagtcg
    26761 caccggtggt tgaagaaact gccgctgccg aaccaattgt tcaggaagct ccagctccac
    26821 gcacagaact ggtgaaagtc ccgctgccag tcgtagcgca aactgcacca gaacagcaag
    26881 aagagaacaa tgctgataac cgtgacaacg gtggcatgcc gcgtcgttct cgccgctcgc
    26941 ctcgtcacct gcgcgtgagt ggtcagcgtc gtcgtcgcta tcgtgacgag cgttatccaa
    27001 cccagtcgcc aatgccgttg accgtagcgt gcgcgtctcc ggaactggcc tctggcaaag
    27061 tctggatccg ctatccaatt gtacgtccgc aagatgtaca agttgaagag cagcgcgaac
    27121 aggaagaagt acaagtgcag ccgatggtga ctgaggtccc tgtcgccgcc gctgtcgaac
    27181 ctgttgttag cgcgccagtt gttgaagaaa tggctgaagt cgtagaagcc cccgtaccgg
    27241 ttgccgaacc gcaaccggaa gtggttgaaa cgacgcatcc tgaagtgatt gccgccgcgg
    27301 taactgaaca gccgcaggtg attaccgagt ctgatgttgc cgtagcccag gaagtcgcag
    27361 aacacgcaga accgatggtt gaaccgcagg aagagacggc agatgcagat attgaagaag
    27421 ttgccgaaac tgctgaggtt gtggttgctg aacctgaagt tgttgctcaa cctgccgcgc
    27481 cagtcgtcgc tgaagtcgca gcagaagttg aaacggtggc cgcggttgaa ccagagatca
    27541 ccgttgagca taaccacgct accgcgccaa tgacgcgcgc tccggcaccg gaatatgttc
    27601 cggaggcacc gcgtcacagt gactggcagc gccctacttt tgccttcgaa ggtaaaggtg
    27661 ccgcaggtgg tcatacggca acgcatcatg cctctgccgc tcctgcgcgt ccgcaacctg
    27721 ttgagtaata attagctcaa agtaatcaag ccctggtaac tgccagggct tttttatttc
    27781 atgtttgaat caacatatta aaattcatca aatggcatcc ttgctaacca acaatgcaaa
    27841 ataggcaaaa aaaatgcccg ccccggcgat cccagagcgg gcagacaaaa catacccagt
    27901 ttcatgatat gttttaaagc gagcttattt gttgagctgg aagagcgaca atccctgcat
    27961 atcggtaaat gctttatacg atgcctgcaa tgccgtttgc tgcatgatgt aagatgaaat
    28021 agttgcattc cagtccacat caaccagatc gctcatctgc tgcgtttgcc ctaaagcgcg
    28081 atcgctacct aaggaatcca gcgactccag tttgttcagc tgcgtgccta attccgcgcg
    28141 aacggtaagg acattattca gcgagttttt cagtccacgg ttggttttat ccaatgccgc
    28201 agcggcgatt tctttatccg cttcgctatc cgcgaccggc gttttcaggg ctgcgatggc
    28261 gctatccagc atggcaaaaa gattggtttc agaagcgcta ccgtctggtt ccgctaccgc
    28321 gttactggta atactgtcga aaattttgtc gcccgtatgt cctatcacca tcgaacgcga
    28381 agcatcgacc tgttgcttaa tactttctgt tccgccaaca taatcaccgt tagcctcact
    28441 aaacggcgca gtctctgttt tatatgtagc gcccattacc gtcagtggtg ttcgccagat
    28501 tcagcaactg gtcacgaagc ccctgaatat ccgtagccag cgaggcccgg tcatcgtcac
    28561 tcaaggtgcc attgctggcg tagacaattt tttcctgagc attctggata gcagtggtga
    28621 cctggctaag tacactctct tccagtgaca ctttttgagt ggcgaaagta cgtgccagcg
    28681 tgtactggct gttttgcgcc tgtgcctggg agagaacaac agcttgtgat gcagcaatgg
    28741 gatcgtctga agggttaacg actcgcttac ccgtcgacat ctgctcgccg tacttcatcc
    28801 attctgcctg agaattggtg atgccgcgca tgttttgctg gtacatcatc tgggtactga
    28861 aacgcatttt atctcccctt agcgaatgtt aatcagcgca tcaaaaatcg cgtttgccgt
    28921 ctgcagaacc tgcgcattcg ccaggtaata ctgctgaaaa cgttgcagat ttccgtactc
    28981 ctcatcgaga ttgacaccgg aaatcgactg ctgctgattg gaaagctgcg tcaccacatt
    29041 accttgcgtg gtgctactgg ttttcaacgt cgcggtttta ttaccgatat cactcactaa
    29101 cgaggcataa gcgtcgttaa aggatttcgc accgcccacc gttttactgt tgctttgcag
    29161 atccagcagg gcctgaccgt tgcggttgtc gctatcaccc gcatcttctt cgctcgccat
    29221 cgcgattttc gcttcgtcgg tgattaatac atccatgttg acgatggcgt cacttactgg
    29281 tttcagcgtg aagctgtcgt taacggcagg cgttcctgta aacgtcaact ccagaccatc
    29341 aaatgccact ttaccgttgg catctggtgt cgccgtaaaa gtggtattgc tggcaaggcg
    29401 ggtgacctgc cactgattat tatcgaacga gattttgtaa tctgtcgcca gtaccacgga
    29461 ggcatcagtt accgtggcac cgatcgcaac gtcacctttg tttttcgtgt tttgcagaac
    29521 cgcgggctta ccgatagtaa agaaatcttc accagcatcg ccgttagcat caaatccggc
    29581 tttgtgttgg ctgttgaaag cctcggcaaa tgccagcgcc agttgtccaa gcgtattacg
    29641 cgtctggtcc agatcctgag aacggaatgt cagaatgccg cccagcgacc cggtattcag
    29701 taatttctcc gggatctcaa tattgcctgc cgtcccatca acgtaagcga cagtcgtacg
    29761 agaagggtcg gcgctggaag gaactgccgc cagttgccgc gccgtacttc cctgaaccag
    29821 tgagtaacca ttggcatcgt gatgttataa gtaccgccat cctgaacgct gacttctacg
    29881 ccaacaatct ggtttaattc gctcaccagt tgatcgcgtt gatccagcag attgttaggt
    29941 gacgcccctg cccccacgcc tgtcaggcgc gagatttgat cgttcaggct ggcaatttgt
    30001 ttagcgtaat tgttgatctg atcaacgctg gcacctatcg cgatattgac ctgtttgtcc
    30061 tggtcgcgca gatattgatc ggtggtttta aactgattca ccaacccttc tgatttccca
    30121 atcagcgcct ggcgcgccgc cgggtcttcc gcgttactca ccagcgtttg caggctggtg
    30181 aagaaatcct gcatctgtgt tgccagcgaa gaggtactgg tggagagcat attgtcgatt
    30241 ttcgacatct gctcatagcg ggcggtcagg ccgctacttt gcgtctgcgc agcacgtaac
    30301 tggttggtaa taaacgtatc atactcacgc tgcacaccag aaacgtagac gccattgcca
    30361 acccagccgc cagcgcccaa cgtgctattg gcctgcgcca taatagtggt ttggcgggta
    30421 tatccggcaa cgttatagct ggagatatta ttacttgccg tatttaacgc cgcctgggcc
    30481 gcgttcagtc cgctcatggc gttattgatc aagctggaca tggaggttcc ttataagcct
    30541 tcaattacag agtattatcg gcagcgaccc gccggacttg agttattcag aacagattat
    30601 caatgttcat gctgtaggtt ttgctcacct tgtcgcttat cgatttcatc tgctggatca
    30661 tgttggtgag tttacgggca tagtgaggat cggtagcata gcccgcgtcc tgtaggacct
    30721 gcgccccctg ttccgcactc gcggcggtcg tcacggcggc gtagcgcggg ttacgtgtta
    30781 acagcccaac gtaatccgac aatgcttcca gatacgagct gtagacccga aacttcgctt
    30841 ttactttctt cgcttcgccg ttttcatatt cagtcgtggt gatttcagtg acttgccctt
    30901 tccagttgcc agaggctttg acaccaaaca ggttatagct tggctcgccg ttttcgcggc
    30961 ggatttgccg ttgcccccaa ccagattcaa gcgcagcctg agcgaggatc aaatgatgtg
    31021 gcacaccgct ttgctggctt gccagttggg cgggcagcga gagttgcgcg aggaatgctc
    31081 tactgtcacc cggcagcgaa tcatcgtagt tacgtggtac ggccttttgc accagctgcg
    31141 aaagcgtctg gttttgataa cgcaccacgg tttcgagcgg gaatttcatc ggtgctgctg
    31201 gcgtggactc ctctggcaat ggttgttctg gcgtcatctg tttaaccatc atctctgcaa
    31261 gccccagacc tttgcccgcc gtcatctgtt gggcaatttg ctggtcatac atactggtat
    31321 acagacgagt gtgctcgctg ctgaacaggc catcttttgg taaagcgtcg cgcatgcttt
    31381 tcaacatcat ctgcacgaac atcccttcca cctgacgggc caccggacgg atatttgccg
    31441 ccggatcttc gctcgctttc gcctttagtt cgttgagtga ttgcgcatcc caggccgcac
    31501 ttgccagtag tttgctgtcg ctgatcatca gatgatttcc agttttgccc gcagacatcc
    31561 cgcactttgc attgattgca aaatagacat cagatccatc ggcgtagcgc ccagcgcatt
    31621 gagcgcacgc accacgttat tgaggctggc gctggaacgt acgctttgca gcgaaccgcc
    31681 gctctggcgt aaatcgatct gcgtttgtgg agtaacaaca gtctgtccac caccaaacgg
    31741 tgtatctggc tggctgacat tggcctgacg attaactgtt actgagagat ttccctgcgc
    31801 taccgcgcag ctgtcgaggg tcacttcgcg attcatcacc accgaaccgg tgcgcgagtt
    31861 aatcactact ttagcgtcct gcggggtgac attaacctgc atattctgga tatcggcaag
    31921 gaaacggacc tgggaactgt tgccacttgg tacgcgcacc tgaatagtcc gcgcatctaa
    31981 cgcggtggcg ctgccatatc cacgcacgcg gttgatggtg tcagcgattt gctgcgccat
    32041 gctgaaatct tcgtcgttaa gttgcaaatt aagggtattc ccgacgccaa actggctggg
    32101 caattcacgt tcaataaccg caccattggt gatccgtcca ccgttcagtt ggttaacctg
    32161 aacactgcta ccgcctgcgg aggctcctgc gccgccaacc agaatattgc cctgcgccag
    32221 cgcatacacc tgactgtcaa cgcccttaag cggagtcatc aacaacgtac ctccacgcaa
    32281 gcttttggca ttacccatgg aagaaaccac cacatcgatg gtttgcccct gacgtccaaa
    32341 cggaggaagt gacgctgtca ccattaccgc agcgacgttt tttagctgca tattggtgcc
    32401 cgtcggaacg gtaattccca gctgtgagag catgttatta agcgtttgtg tggtaaacgg
    32461 cgtctgggtt gtctggtcac cagtgccatc cagccccact accaagccat agccaatcag
    32521 tgagttttgc cttaccccct gaacactggt gagatcgcga atacgctcag cctgagccgc
    32581 cgtcgtaacc agtagaagaa ttaatgcaga gagaaattta atcaccacag cctcacttac
    32641 attggcgaca ggttaaggaa gaaacgctgc aaccagccca tattttgcgc ttcgttaatg
    32701 tagccattgc ctacgtattc aatacgcgca tccgccacct gagtagacgg tacggtattg
    32761 ctgccgctga tagtgcgtgg attaaccacg cccgagaagc gaataaattc ggtaccctga
    32821 ttaatggcaa tctgtttttc acccaccaca tgcaggttgc cgttgaccag tacctggtca
    32881 accgtcaccg tcaacgtgcc gctaaaggta ttgctggcat tggccccgcc ctttccgttg
    32941 aacgtgttac caccggaggc ttcgacatcg gcacgagcgt taccaaacag cccctgcaaa
    33001 tagcgcggca cagtatcaaa gccaaaatta gttttaccgt cacggctggc attcgcagag
    33061 gagcttttgc tggcgctgac gttctcctgc aacacgatgg tcagcgtatc gccaatattg
    33121 cgtggtcgac gatcttcaaa cagcggttga tagccatagt taatcggctg agcagactgg
    33181 aaaatagaac cgttggcgac gggcgtcgga ccgggaaccg gttgtgcact ggtcgccccc
    33241 tgcaccagcg gcgtggaggg tatccaggcg cagccggtta gtgaaagcac caacaagctg
    33301 gaaatggcat aagtatgcgc agcgtttttt tgcatggcta tcatcttcaa aaatcagtaa
    33361 accggtgaac ctgccaccgg ttatgcctta gagttgcgtc agtttttgca gcatctgatc
    33421 ggtggtggac accgctttac tgttgatttc gtaagcgcgt tgcacctgaa tcatattgac
    33481 cagttcttcc gccacgttga cgttagacgt ttcaacatac ccttgataca gcagtcccgc
    33541 gccgttcagg cccggcgtgc tttcgttcgg tgcaccggag gattgcgttt cggtgtagag
    33601 gttttcgcca atgctctcca gtccggtatc attcataaag gtggtgagat tgagctgccc
    33661 aacctgaacc ggagctgcct ggccttgttg ggttacgctg accacgccat cacgaccgat
    33721 ggtgatactt aacgcattcg ccggaatggt gatcgctggc tgcacctgaa aaccaccagc
    33781 cgtcaccagc tgcccgttct gatccacctg gaaagagccg tcacggatat aggctgatga
    33841 gccatctggc aacattacct ggaaaaagcc ctgcccttta atcgcgacat ctttgctgtt
    33901 attggtctgc gacagattgc cctggctgtg taagcgttca gtggcgaccg ggcgtacccc
    33961 cgtgccgatt tgtaatccgg agggtaaggt ggtttgttcg gaagactgtg cccccggctg
    34021 gcgaatggtt tgataaagca gatcttcaaa caccgcgcgc tgacgcttaa aaccgttagt
    34081 actgacgttt gccaggttgt tggcaatgac gtccatattg gtttgctggg cgtcaaggcc
    34141 cgttttggcg atccataatg aactgatcat ataatgtcct gtagtggatt gctgacaagc
    34201 agagcagaag cacgcgacag tgtgaatttc ccctcaccct aaccctctcc ccaaaggggc
    34261 gagggaatag tccgcgcgct tttattgcaa tgtcatcaat cgttcaatta actcatcgac
    34321 agcagttggt tggcacggcc tgcgttatca tcgacgctgc tgatcacctt catctgcatt
    34381 tcaaaacgcc gcgcgctggc aatcatgtcg ctcattgccg caacggcatt gacgttactg
    34441 ccttccagaa cccccgacat cacacgcaag gttggatctg cctgcagtac cggccctcgc
    34501 gtggcctggg tttctgcgct taaacgaaaa atgccgtcgt caccgcgctg cacttcgctg
    34561 cccgtggctt tcaccagttt cagacgccct actggcgcaa ctgtatttgc cggatcgccc
    34621 ggattgagcg ccgagattgt gccatcggca gcaatcgtga tttccgcccc ttccggcaca
    34681 gcaattggcc ccgcctcgcc tatcaccgga tgcccctgaa ttgtcagttg cccggtggga
    34741 tcaacctgaa tgctgccatt acgcgtatac ccttcgctgc cgtcagcggt ctgcacggcc
    34801 agccatcctg ctgcaacgca acgtccagcg ggcgagaggt gtaatccatc ttgccgggcg
    34861 tcatgtctgc acccggtgtt gacgccgtta ccagcgtgcg cgtgggcagt gaaagccctt
    34921 ccaccggcac tgcgcgcaag gcgtttaact gcgcgcgaaa accgggcgta gaggcattgg
    34981 ccagattact ggcggttacc gcctgttgat tcagcgtctg gctggctgct cccatcgcag
    35041 tataaattgc gtgatccatt gagctatccc gtcagcgatt agcgtaagtt aacccgcgtg
    35101 ttgaggatct ggtcctgggt tttgatggtc tgggcgttag acttatagtt acgctgggca
    35161 acgatcatat tgaccagttc tttactgaga tcgacgttgg acgcttccag cgcaccgttg
    35221 gtcagagtgc caaagtttcc cgtcccggct gtccccaaca gcgccacgcc agaagattgc
    35281 gtcgcagacc agacgttgtc gccttcggat gccagacctt cgttgttggc aaagttcgcc
    35341 agtacaatct gccccagcag ttgggtttgt tcgttggaat tgttgccgac aaccgtaccg
    35401 tcatcattga tttgataact caccagatcg cccggtttgt agccattctg ggtggttgcc
    35461 acaatgttgt tagcgccggt tgcgatatta ttcgccatcg caccatccac taatgtgcca
    35521 ttagcattaa attccagtgt tgtcgctgtc ttcgcaatgc tgtttggatc actgctatcc
    35581 tgggtgtaga cctgccagtt attatccccg gtcttcacaa aatagacgct catgtcatga
    35641 gcattaccct gactgtcgaa aacagtcacc gaaccttttt tgttatagct atccgcattg
    35701 ctggcgctga atggcgtaac agtaggaagc ggatcactgg aattcaggtt gatctgcatc
    35761 gacgccgtgg tggtagtttt cgctgccatc agggtattcg ggatcgaaat attggtcgga
    35821 ttcgcccctt gctgaatagt cggcggcgta ccggttaccg ggtaacccgt cagctgtaaa
    35881 ccttgcgtat tcaacaggtt acggttttca tccagcttaa attgtccgtt acggctgtag
    35941 aacaccgaac cgttgctgtc taccagacgg aaaaaaccgt tctggctgat agcaacgtcc
    36001 agacctcgcc cggtgttggt ggtcgtgcca tcggtaaagt cctgagtgat accggcaact
    36061 tttaccccca gtcccacttt cgaaccggca aacatatcgg caaaagaggc cgtgcctgat
    36121 ttaaagccgt aggtggcgga gttggcgata ttgttgccaa taacatcgag gttggtggcg
    36181 gcagcgttta atccgctaac cgcttgagaa aaggccatga ctgactcctg aaatgtgaag
    36241 gcttaaatta tctgccgtac ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc
    36301 gtattaccgc tgttgccgcg gatcacaccc tgcaccagag caaactgcag cggctgggca
    36361 accagttgtg taccaccgtt actggcgcta atcgctacat tgtaagaacc gttcggcgca
    36421 gtgctgccat cagtcaacga accgtcccag gtgaaactgt gaactccggc ggtcagttca
    36481 ccaatatcaa tggtgcgcac aaccgcgcca tttttatcgg tgatggtggc cgtaactttg
    36541 tctgccgcct gttgcagctc aacaccaaac ggcgtggtcg tggtcacagc cccttcttca
    36601 ctgccggttc ccgcaagaac agttgtgccg gggatcatca cgccgtgacc gatcaggtta
    36661 ctggcctgta acgactggct gttatcaatc tgtccggaaa tagatccgag cgtggtattg
    36721 agtttttcaa tcccgctgac cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt
    36781 tccattggat tggtcgggtc ctggtttttc agctgcgcca ccagcaaagt cagaaaactg
    36841 ctttgtaaat ctgcggcgtt gctgcccgtg agcgaactac tgctggtggt actgacgccg
    36901 gtatttgtcg gatcggtggt ggttaccgca atggacatag ctttctcctt tattgaccga
    36961 gcgtaagggt tttcagcatc atgcttttca ccgtgttgag cacttcaaca ttggcctgat
    37021 agctgcgtga cgccgacatg gtgttaacca tctccccgac gacatcaacg ttcggcattt
    37081 ttatgtagcc ctttgcatct gccagcggat tacctggttc ataaaccagt ttgtccgggg
    37141 cctgactttc ttaacatcgg caacctttac gccgcctgtc gcagcacctg gtgcagcgtt
    37201 aacctggaat accacctgtt ttgcccgata tggctgtcca tcaggaccgg tcacgctatc
    37261 agcatttgcc agattactgg ccgccacgtt caggcgctgg gactgggcag ttaacgccga
    37321 cccggcgata tcaaaaatat tcagcagtgc catccgttaa tttccgctct gtaaaacgtt
    37381 catcatgcct ttgatttgcc cgctcaacgc gctaaggctc atctggtatt gcaggctgtt
    37441 atcggcaaac tgggtgcgtt cgcgatccat atcgacggta ttaccgtcaa gcgaaggctg
    37501 gtccggaata cggtattgca gttctgcggt aggaggcgtc agcgcctgcg ccgggatgtg
    37561 ctgcgttgag gtcatcgtca gtgcaaccac actggttgca tcccgtccac gttgcatgac
    37621 ttttttaagt tcactggcaa aatcgatatc gcgcgcctga taaccagggg tatcggcatt
    37681 ggcgatgttt gctgccagca cttcctgacg ctgggcgcgc agattgagcg cctcttgttg
    37741 aaaacgtaag gcggcgtcga gcttatcgag catatctcct ccgcaggtat caaaattctg
    37801 ccatcagctt aaatgccttt acgcgcgcgt tatcggcgga ataaacgcaa aatgggtcgc
    37861 tatttatgcc gttgatggtc attgcggaca ggtacaattc acgttgtaga aatggctggg
    37921 ggcgaaaatg ctgacaataa aacgtagcgt ggcgatcatc gcgatactgt tcagtccgtt
    37981 aagtacggcg agcaatctca catcgcaatt gcacaacttt tttagcgccc aactcgcggg
    38041 ggtaagtgat gaggttcgtg tttctattcg tacagcgccc aatctactac cgccatgcga
    38101 gcagccattg ctttcgatga gcaataattc ccgcctgtgg ggcaatgtga atgtgttggc
    38161 acgctgcggt aacgacaaac gatatttaca ggttaatgtg caggccacag gaaattatgt
    38221 ggttgccgcg atgcctattg tgcggggagg aaagctggaa gctggcaatg tcaaactgaa
    38281 acgtggacgc ctggataccc tgccaccgcg tacggtgctg gatatcaatc aacttgttga
    38341 tgccgttagc ctgcgcgatc tatcacccga tcaacctatc cagttaaccc agtttcgcca
    38401 ggcatggcgg gtaaaagcgg gacaacgcgt caatgtgatc gccagcggtg atgggtttag
    38461 cgccaacgca gaaggtcagg cgctgaacaa tgcagccgtc gcacagaatg cgcgggtgcg
    38521 catggtatcg gaacaggtag tcagcggcgt tgttgatgca gatgggaata ttcttataaa
    38581 cctgtaagct gttaaagatt acccgtccct tgccgataaa taagcaacac atgataaaag
    38641 cgccctcaat gaggaataaa ccatgagtat tgatcgcact tcgcctctga agcctgtaag
    38701 caccgttcaa ccgcgcgaaa ccaccgacgc gccggtaacg aacagccggg cggcaaaaac
    38761 aaccgcctcc accagcacca gtgtgacgtt aagcgacgcg caagcaaaac tgatgcaacc
    38821 cggcagcagt gatatcaatc ttgaacgtgt cgaagcgtta aaactggcga ttcgtaacgg
    38881 tgaactaaaa atggacaccg gcaaaattgc cgatgcgctg atcaacgaag cgcagcaaga
    38941 cttgcagagt aactgatcgt atgacacgtc ttgcagagat cctcgaccag atgtccgctg
    39001 tgcttaacga tctcaaaacg gtaatggatc aagagcagca acatctctct atggggcaga
    39061 tcaacggcag ccagttgcaa tggattacag aacaaaaaag ctcactgctg gcgacgctgg
    39121 attacctcga acagttacgc aagaaagaac ccaatacagc aaatagcgtt gatattagtc
    39181 aacgctggca ggaaattact gtgaaaacgc agcaactacg ccaaatgaat caacataacg
    39241 gctggttact ggaaggacag attgagcgca atcaacaggc gctggaaatg ttgaaaccgc
    39301 atcaggaacc gacgctatat ggggcgaacg gtcagacctc aacaccccat cgcggcagta
    39361 aaaagatttc gatctgaata ttatctccgg cctgcactgc aggccggaat gcattgttac
    39421 accgtccggc gggcaaattc tttaactttg aagcccagta ccgccagtgc agcgaagtac
    39481 gcggcaatcc ccgccagcac gaccgccatt aaacgcagta agcgccaggg catggtaccc
    39541 aatgaccact ccggcatgat atgtaacata cctaaaagca cgccagacat caccagtacc
    39601 gccaccacca gacgcaacag aaacgccatc cagccgggtt gcggagtaaa gattttctgc
    39661 ttacgcaact gccagtaaag cagcgaagca ttcagacacg ccgccagacc aatagaaagt
    39721 gacagcccgg catgtttcaa cggaccaata aacgccaggt tcatcaattg cgttaaaatc
    39781 agcgtaacga tggcaatttt cactggcgtt ttaatgtcct ggcgggaata aaagccagga
    39841 gccaacactt tcactacaat caggccaatc aaacccaccg agtaggcaat taacgcccgc
    39901 tgggtcatca gcgcatcaaa cgcggtaaat ttaccgtact ggaacagcga aacggttaac
    39961 ggacctgaaa gaatgcccaa cgcaaccgca ctcggcagtg ccaacaggaa acaaagacgc
    40021 aacccccagt ccatcaaacg gttgtattca tcatgattgc cactggcaaa acttttcgac
    40081 agcgacggca gcaaaatggt gccaagcgcc acgcccagca caccggaggg aaactccatt
    40141 aagcggtcgg cgtaatacat ccaagacacc gaaccggaag caagaaacga ggcaaaaatg
    40201 gtgttgatga ttaaggagat ctggctcaca gagacgccaa ggatcgccgg tcccatctgt
    40261 ttcaccacgc gcattgctcc agcatcgtgg aagttaatgc gcggcaggac cagcatgccg
    40321 atcttcttca ggtgcggtag ctgatacacc agctgcagaa tgccgccgac cgtaacagcc
    40381 cacgccagcg ccagcaccgg tgggttaaag tacggtgcgg caaacagcgc gaaaccaatc
    40441 atgctgatgt taagcagtgt tggcgcaaac gccggaatcg agaagcggtt ccacgtattc
    40501 agaatcgctc ccaccagcga cgccagggag atcagcaaga tatagggaaa ggtaatcttt
    40561 agtagctggc tggtcagggc aaatttgtca gctgtgtcag cgaagcctgg cgcggtcacc
    40621 atgatcaccc acggtgcggc gagcatgcca gcgaccgtca caaccgccag cgcaagtgtc
    40681 agcaggccag aaacataaga gacaaagacc cgcgtggcgt cttcaccctg cttactttta
    40741 tattccgcca gaatcggtac aaatgcctgg gaaaatgccc cttcggcaaa gatacggcgt
    40801 aacaagttag gaagtttaaa agcgacgaaa aaggcgtcgg ttgccatccc tgcgccaaag
    40861 attctggcga caattgcgtc tcgtgcgaag ccaagcacac gcgaaaacat ggtcatcgag
    40921 ctgacggcgg ccagcgattt taataaattc atcggtgttc taatccggac ccacgggcaa
    40981 aacgcctgca aggcaggcgc tgaaagaggc gaatagtcta cctagattat ggtgaattgc
    41041 taccgccaga tgttacaggg tttattcact catcgcatcg cgccagatct tgtcaacgat
    41101 acgttgcgcc agcactgcct attcgccggc ggtttgcgga actgtctgat tttacacgca
    41161 ttcaatgaag tgccgcgcac agccgacaaa tccacgttgc tcaagagtgc tctgccaacc
    41221 aggaatcggt ttattcacta caccctgccc gcgctcctca cgccattcgc gcatatccgt
    41281 aatgtcgatg agcgcaccgt cagtcacggc ctgcacggtt tcacgctgac ttccggcacg
    41341 gcgatgcata caagtggtga tctgcaaagg accggccgaa aaatggtgct cggcaaacag
    41401 catttcgcca gcgtcgttag tcagtagcgt accgccatcc agagaggctt tgccgcccga
    41461 caaccacagc gcggtatcca ccacatgcag ataatcatcc agcaacgtga aataaagatc
    41521 gtgtggcccg acgctattgc tacgatgttt atccattctt agcgaggctg cggtggcgag
    41581 ttgcgttttt aactcaccgt agagtggtgc gaaacgacgg ttaaaaccga ccatcaacgt
    41641 cagttttttc cgcgccgcca gttccaccag cctttcagca tcgcacagat tttctgccag
    41701 cggtttatcg acacagacat gtacccccgc attgagtaac gtactgacca cgtcaaagtg
    41761 gctggcggtg ctggagtgca caaaaaccgc atcacaactg gcggcaaggc tggataacga
    41821 atcggcataa ggaatgcgcc agctgtcaca aattggcagg gctttggcgc gcgtaggcga
    41881 ccaggctcct tgtaacgtcc agtcagacgc tgccgccagc accggtaacc acgctttttg
    41941 cgcaatgccg cctaatccca ctacgccgat acgtaatttt ttcactttaa tctcccagat
    42001 gggccagcaa cgaatcaaga cgttgtttca gttctgccac ttcgatttcc agggcttcga
    42061 cgcgggtctg taaatcaccg tcaaccatgt tcgacatagc cgttaccgcc ggctgatctt
    42121 caacctcacc actgaaaaga tgcatgtagc ggctttcgcg tttacccggt tcgcgggcca
    42181 gacgcaccac aaaaggacca tcttcgcgat tcgccagttg ttccagcgtc gactccactt
    42241 ccgccatatc gctgaattca tacattcgcg cggcgcggct gcgcagttca cccggcgtct
    42301 gggcaccacg caataacaac gtggtgatta acgccacttc tgcggcgctc agtttcagat
    42361 cgccaaattc tgaattacaa aaacgttgct catatttggt gacccgatta ccaaaaccgc
    42421 tcactgtgcg tagataatga cgtttgacca gattatccag ctgttcctgc acttcggatt
    42481 cactcagatt catgaccggt tcacggttcg ttttctgatt acaggccgtg actacaccat
    42541 tgactgagag cgggtattgc tccggcgtgg tcacctgctt ttccagcaag caaccaatta
    42601 cgcgagcttc cagggcagta agttgatatt tcatgagatt ctccttagcg gccgggcgtc
    42661 cagtctgggg tagttaatgc cgtcagtacg tgatcgcgcc attgtccatc aatcaacaga
    42721 tagtctttcg catagccttc tttttcaaaa cccagtcgcg ccagtaaatc accgctgcgt
    42781 ttattgtgcg gcatataatt agccataatg cgatgaatat gttgggtgcg ctgcatataa
    42841 cgaatggctg cggtcagggc ttcaaacatg agtcctttgc cctgccattt ttgcccaatc
    42901 gaataaccga gatagcaggc atgaaaagag ccgcgaacaa cattggaaaa attggcaacg
    42961 ccaataatct ctttttcgtc cgggtcgaat aagccaaagt agaaagctga accttgttta
    43021 tgaaattcgt taatcatccc tagcctggcc tgccagcctg atggataaca gtggctttcg
    43081 tcgcgcactg gctcccaggg cttgaggaaa tggcgattct ctgcgtaata atccgccaga
    43141 cgccaggcat cacgatcatg caccagacgc acgaccagtc ggtctgtggt taagcgcact
    43201 tttggcacgt tactgcgata gccaaacatt ctatacctac tccttcccgt aacgtcttta
    43261 atacgctgta agtattacta tacctgcgca accagaccct gtgaaaagtc caaaatgcca
    43321 ttttctgcat tattcacata tttgataaaa actctctatc aaagcgacgt tttgataaaa
    43381 aaatactgtc cccgaagtga tgggaaaagt cttagcggca atgcagaata gaaagtgctc
    43441 atcttttatt tcccgggagg ggaaatgtcc cgcgtatcgc aggcgaggaa cctgggtaaa
    43501 tatttcctgc tcatcgataa tatgctggtc gtgctggggt tctttgttgt cttcccgctg
    43561 atctctatcc gcttcgttga tcaaatgggc tgggccgccg tcatggtcgg tattgctctc
    43621 ggtctacgcc agtttattca gcaaggtctg ggtattttcg gcggcgcaat tgccgaccgc
    43681 tttggtgcca aaccgatgat tgttaccggt atgctgatgc gcgccgccgg attcgccaca
    43741 atgggtatcg cccacgaacc gtggctattg tggttttcat gcctgctctc gggactcggc
    43801 ggcacgttgt ttgatccgcc gcgttcggcg ctggtggtga aactaatccg tccacaacaa
    43861 cgtggtcgtt ttttctcgct gttgatgatg caggacagtg ccagtgcggt cattggcgca
    43921 ttgttgggga gctggctgtt gcaatacgac tttcgcctgg tctgcgccac aggggccgtt
    43981 ttgtttgtgc tatgtgcggc gttcaatgcg tggttgttac cggcatggaa actctccacc
    44041 gtacgcacgc ccgttcgcga aggcatgacc cgcgtgatgc gtgacaagcg ttttgtcacc
    44101 tatgtcctga cgctggcggg ttactacatg ctggctgtac aagtgatgct gatgctgcca
    44161 attatggtca acgacgtggc tggcgcgccc tctgccgtta aatggatgta tgccattgaa
    44221 gcgtgtctgt cgttaacgtt gctctaccct atcgcccgct ggagtgaaaa gcattttcgt
    44281 ctggaacacc ggttgatggc tgggctgttg ataatgtcat taagcatgat gccggtgggc
    44341 atggtcagcg gcctacaaca acttttcacc ctgatttgtc tgttttatat cgggtcgatc
    44401 attgccgagc ctgcgcgtga aaccttaagt gcttcgctgg cggacgcaag agctcgcggc
    44461 agctatatgg ggtttagccg tctgggtctg gcgattggcg gcgctattgg ttatatcggt
    44521 ggtggctggc tgtttgacct gggcaaatcg gcgcaccagc cagagcttcc gtggatgatg
    44581 ctgggaatta ttggcatctt cactttcctt gcgctgggtt ggcagtttag tcagaaacgc
    44641 gccgcgcgtc gtttgctgga acgcgacgcc tgatttgata ctcccccctg actcctccat
    44701 actgaaagtg aactgacagc caaatggagg agtcacgtga agctatacat ttacgatcac
    44761 tgcccttact gcctcaaagc ccgcatgatt ttcggcctga aaaatatccc cgtcgaatta
    44821 catgttctgc tcaacgacga cgcagaaaca cccacccgga tggtcggtca aaaacaggtt
    44881 cccattctgc aaaaagatga cagccgctat atgccagaaa gcatggatat cgttcactat
    44941 gtcgataaac tcgacggcaa accgttactg accggcaaac gttcccctgc cattgaagag
    45001 tggctgcgca aggtcaatgg ctacgtcaac aaactgctgt tgccgcgttt tgccaaatcg
    45061 gcatttgatg agttttctac tcccgccgcg cgcaaatatt tcgtcgacaa gaaagaggcc
    45121 agcgcgggta attttgccga cctgctggcc cactctgacg gtctgattaa gaatatcagc
    45181 gatgatttgc gtgcgctgga caaactgatc gtcaaaccga acgccgtgaa tggcgaactt
    45241 tcggaagatg atattcagct atttccgcta ctgcgtaatc tgacgctggt agccggaatc
    45301 aactggccaa gccgcgttgc taattaccgc gataatatgg cgaaacagac acaaatcaat
    45361 ttgttatcat caatggcgat ttaatcctga cccgccgcgg cagcctgtat tcgcgggttg
    45421 ccgccgtttt cagttttttc tgctggcgag tttatcgctg gtgacacatg ctgcgttgtg
    45481 tcaggatatt gacgcagatt tcatgtcgag gactaccatg aacaagtttt tatttgccgc
    45541 tgcattgatc gtcagtggcc tgctcgttgg ctgtaatcaa ctcacccaat acaccatcac
    45601 cgaacaagaa attaaccagt cgcttgcgaa acataataat ttctcaaaag atatcggttt
    45661 acccggcgtg gctgacgccc atattgttct gacaaacttg accagccaaa ttggtcgcga
    45721 agagccgaat aaggttaccc taaccggaga cgccaatctg gacatgaact ccctgttcgg
    45781 tagtcagaaa gcgaccatga aactgaagct gaaagcgctg cccgtgtttg ataaagaaaa
    45841 aggtgcgatc ttcctgaaag agatggaagt ggtcgatgcg acggtacaac cggaaaaaat
    45901 gcaaacggtg atgcaaacgt tgcttcccta tttgaaccag gcattacgca attactttaa
    45961 ccagcaacct gcttacgtcc tgcgcgaaga tggcagtcag ggcgaagcaa tggcgaaaaa
    46021 actggcgaaa ggcattgaag tgaagccagg cgaaattgtc attccgttta cggattaatg
    46081 acgagggcgc attcgcgccc tttatttttc gtgcaaagga aaacgtttcc gcttatcctt
    46141 tgtgtccggc aaaaacatcc cttcagccgg agcatagaga ttaatgactg caccatccca
    46201 ggtattaaag atccgccgcc cagacgactg gcaccttcac ctccgcgatg gcgacatgtt
    46261 aaaaactgtc gtgccgtata ccagcgaaat ttatggacgg gctatcgtaa tgcccaatct
    46321 ggctccgccc gtgaccacag ttgaggctgc cgtggcgtat cgccaacgta ttcttgacgc
    46381 cgtacctgcc gggcacgatt tcaccccatt gatgacctgt tatttaacag attcgctgga
    46441 tcctaatgag ctggagcgcg gatttaatga agccgtgttc accgctgcaa aactttaccc
    46501 ggcaaacgca accactaact ccagccacgg cgtgacgtca attgacgcaa tcatgccggt
    46561 acttgagcgc atggaaaaaa tcggtatgcc gctactggtg catggtgaag tgacacatgc
    46621 agatatcgac atttttgatc gtgaagcgcg ctttatagaa agcgtgatgg aacctctgcg
    46681 ccagcgcctg actgcgctga aagtcgtttt tgagcacatc accaccaaag atgctgccga
    46741 ctatgtccgt gacggaaatg aacggctggc tgccaccatc actccgcagc atctgatgtt
    46801 taaccgcaac catatgctgg ttggtggcgt gcgtccgcac ctgtattgtt tacccatcct
    46861 caaacgcaat attcaccaac aggcattgcg tgaactggtc gccagcggtt ttaatcgagt
    46921 attcctcggt acggattctg cgccacatgc acgtcatcgc aaagagagca gttgcggctg
    46981 cgcgggctgc ttcaacgccc caaccgcgct gggcagttac gctaccgtct ttgaagagat
    47041 gaatgctctg cagcactttg aagcattctg ttctgtaaac ggcccgcagt tctatggctt
    47101 gccggtcaac gacacattca tcgaactggt acgtgaagag caacaggttg ctgaaagcat
    47161 cgcactgact gatgacaccc tggtgccatt cctcgccggg gaaacggtac gctggtccgt
    47221 taaacaataa aaatcgtagc ttcctgttgt cattaggtta ttttacctgt ataaataacc
    47281 agtatattca acagggggct attatgcgaa ttgaagtcac catagcgaaa acttctccat
    47341 tgccagctgg ggctattgac gccctggctg gcgaactttc ccgccgtatt cagtatgcgt
    47401 ttcctgataa tgaaggccac gtatcggtac gttatgccgc agcgaataat ttatcggtta
    47461 ttggcgcaac aaaagaagat aaacagcgca ttagcgaaat tctccaggaa acgtgggaaa
    47521 gcgccgatga ctggtttgtc agcgaataat atgcagtgat ttttgttttt gccggatcgc
    47581 cccggctttt ttattttact tacgaatttt gagtacaaaa tttctacact tcctcaaaat
    47641 aattgcacaa tatgctaaaa aaatatgaat gacatgctgt tttattgttc atataaatct
    47701 taaaagcaga aatttatctg ttcccccctt taaaaattcg gtgaataccc ttacttattg
    47761 gtatactgaa gttgcttcag ataaaaacgc attgaacctc gaataacgtt gtctagtaac
    47821 acgaattagg gggccatgat ggaaaaaaat aatgaagtca ttcagactca tccgctcgta
    47881 gggtgggaca tcagcaccgt tgatagctat gatgcgctga tgttgcgttt gcactaccag
    47941 accccaaata agtccgagca ggaagggact gaagttggtc agacgctctg gttaaccact
    48001 gatgttgcca gacaatttat ttcgatatta gaagcaggaa tcgccaaaat tgaatccggt
    48061 gatttcccgg taaacgagta tcggcgtcat taacatgaca tgaccatcca cccaaaaggc
    48121 acctcaccgg tgcctttacc atttctgggt tgtatatctg cattcagtta attactctgc
    48181 ttaaaccatg agagcagaga gaaaaaaatg aaatacgatc tcatcattat tggcagcggt
    48241 tccgtaggcg ctgccgccgg gtattatgca acccgcgccg gtttaaacgt gctaatgacc
    48301 gacgcccata tgccaccgca tcaacacggc agccaccacg gcgatacgcg attaattcgc
    48361 catgcttatg gtgaaggcga aaagtatgtc ccgttggtcc tccgcgcgca aacgctgtgg
    48421 gatgaacttt cccgccacaa cgaagatgat cccatttttg tacgctctgg tgtcattaac
    48481 ctcggcccgg ctgactccgc atttctcgcc aacgtcgccc acagcgccga acagtggcaa
    48541 ctcaacgttg aaaagctcga tgcgcaaggg attatggccc gctggccaga aatacgcgtc
    48601 ccggacaact acatcggctt atttgagact gattccggtt ttttgcgcag cgaactggcg
    48661 attaaaacct ggatccaact ggcgaaggaa gcgggctgtg cgcaactgtt caactgcccg
    48721 gtcaccgcaa ttcgtcatga cgatgatggc gtaactattg aaacggttga cggtgagtat
    48781 caggcgaaaa aagcgattgt ctgcgcggga acatgggtaa aagacctgct cccggagctg
    48841 cctgtccagc ccgtacgtaa agtatttgcc tggtatcagg ccgatggccg ctatagcgtg
    48901 aagaataaat tcccggcgtt taccggtgaa ctgcccaatg gcgatcaata ttatggtttt
    48961 ccggcagaaa acgacgcgtt gaagattggc aaacataacg gaggccaggt tatccattca
    49021 gcggatgaac gtgttccgtt tgcggaagtg gccagtgatg ggtcagaagc cttcccgttc
    49081 ttgcgcaatg tattgccggg tatcggttgc tgcctgtacg gcgctgcctg cacctatgat
    49141 aattcgcctg acgaagattt tattatcgat accctacccg gccacgataa tacactgctc
    49201 attaccggcc tgagtgggca cggttttaaa tttgcgtcag ttttagggga aatagctgcc
    49261 gattttgcgc aagaccaaaa aagcgatttt gatttgacgc cattcaggct ttcccgcttc
    49321 caataatcat cattacggcc tccagaatga ggccgttttt acaggagtat ttaatgcgtc
    49381 ggctgttgca ctatctcatc aataatattc gcgagcatct gatgctttat ctttttctct
    49441 ggggattgtt ggcaattatg gatctgatct atgtgtttta tttttagttt tccgcactga
    49501 cataaactga caaatattta atctcccttt catttagcgt gaaaaattga atgagattat
    49561 tcctgcttct acagcactca ccagacatct ctgatgctga acccactaaa cttatgaaga
    49621 ttctttcatt attaattcaa aattattgaa ataaaaataa atatcattca atttgttatt
    49681 tttgtgttgc aattcattct ccgacacgcc attttctttc tctcgcaaat tcgtattgga
    49741 tgcacttatg tcattcacaa atacccctga acgctatggt gttatatctg cggccttcca
    49801 ttggctaagc gcaattattg tctacggcat gtttgcttta ggtctgtgga tggtcacgct
    49861 cagttattac gatggctggt atcacaaagc acccgaactg cataaaagca tcggtatttt
    49921 gttaatgatg gggctggtta ttcgcgttct gtggcgtgtc atatctccac cgccgggtcc
    49981 gctgccgagt tattcgccca tgactcgtct tgccgcaaaa gctggacatc tcgcattgtg
    50041 cctattgctg ttcgccatcg gcatcagcgg ctatctgatc tcaactgccg atggtaaacc
    50101 gatcagcgtt tttggctggt ttgacgtccc cgcgaccctt gccgacgctg gcgcacaggc
    50161 agactttgcc ggtgccctgc atttttggct cgcgtggagc gtcgtagtac tgtctgttat
    50221 gcacggattt atggccctga agcatcattt catcgataaa gacgacactc tgaagcgcat
    50281 gctgggaaag tcgtcatctg actatggagt ataaaaaatg aaaaaaagcc tgcttggttt
    50341 aaccttcgcg tccctgatgt tctctgccgg ttcagcggtt gccgccgatt acaaaattga
    50401 caaagaaggt cagcacgcct ttgttaactt ccgcatacag catcttggct atagctggtt
    50461 atatggcacc tttaaagatt tcgacggtac ttttaccttt gatgaaaaaa atccggctgc
    50521 cgataaagtg aatgtgacaa ttaacaccac cagcgtcgat actaaccacg ccgaacgcga
    50581 taaacatctt cgcagtgcgg attttctcaa taccacaaaa tatccacagg caacattcac
    50641 ctccaccagc gtgaagaaag acggtgacga actggatatt accggcgatc tgacgctgaa
    50701 tggcgtaacc aaacccgtca cgctggaagc gaaattaatt ggtcagggcg acgacccatg
    50761 gggcggtaaa cgtgcaggct ttgaggccga aggcaaaatt aagctcaaag acttcaatat
    50821 caagacagat ttaggtccag cttctcagga agtggatctg attatttcag tggaaggcgt
    50881 acagcagaag taagagtgaa atgttgccgg atggcacgca tccggcaatg atatttattc
    50941 tgttggatca ggaataccca gtgttgtatt cagacgtcca cgagacttat taaagatctt
    51001 attgccattt tcacgtcctg cccgacggcg tcgctgttct tctggcggta acgcgctttc
    51061 ttcgcagcaa atctcactac aacaaccttt gtatttttcc gcgcatactg gacactgaat
    51121 aaacagcagg tggcagccat catttttaca gttggtatgg ctgtcgcacg gcgcgccgca
    51181 ctggtggcaa tgcgcgataa tctcatccga tatacgttcg cccatccgct cgtcaaaaac
    51241 aaaatttttg ccaataaaac gcaccggcaa gccctgctcg cgcgccttac gggcgtattc
    51301 aataattccg ccctcgatat gccagacttt gttgaatccg ttatgtttca tccaggcact
    51361 ggccttttca caacgaatgc cgccggtgca gtacatgacg atttttttat ctttatgtgc
    51421 ctgcatcatc tcgactgctt ttggcagctg ctcacggaag gtatctgccg gaatttcgag
    51481 cgcgttttca aagtgcccca cttcatactc atagtggtta cgcatgtcga taaatagtgc
    51541 atcgggatcg tcaagcatgg cgttcacttc cgccgcttgc agatactcac caacattgct
    51601 ggcatcaaag tgaggatcgt caataccgtc ggcaacaatg cgatcgcgta ccttcatgcg
    51661 cagtacccag aaggatttcc cgtcatcatc caacgcgata ttcaggcgta aaccctctaa
    51721 agccgggtcg aaggcataga gctgcgcgcg aaatgtttca acattgctcg caggtacgct
    51781 gatttgcgcg ttaatgccct catgcgccag atacactcgc ccaaaaacat tcagcgcggt
    51841 aaacagctga tataaagcgt cacgggtcgc cttaggatcg gcgatgtgga aatacttgta
    51901 aaacgaaatg gtggttcgcg gttcgctctc agccaacatt ttggctttta acgcgtcgtt
    51961 ggaaatgcgg ttgtgtaaca ctggcatggt gtacggttcc tgcgagatgg gaaagtaaaa
    52021 atccgcggca tgatatagca attatcgata attaacatcc acacatttta cgctacattt
    52081 gcgcattaaa aattatttgt tatttacaag cgcggcaatt tcgcccagtc ttcagccaca
    52141 aattttggtt gcgggcgaaa aaatgcgaca atacatacaa ttgcccgtat aggttgaaaa
    52201 acaggattga tatgacgaat ctacccaagt tctccaccgc actgcttcat ccgcgttatt
    52261 ggttaacctg gttgggtatt ggcgtacttt ggttggtcgt gcaattgccc tacccgatta
    52321 tctaccgcct cggttgtgga ttaggaaaac tggcgttacg ttttatgaaa cgacgcgcaa
    52381 aaattgtgca tcgcaacctg gaactgtgct tcccggaaat gagcgaacaa gaacgccgta
    52441 aaatggtggt gaagaatttc gaatccgttg gcatgggcct gatggaaacc ggtatggcgt
    52501 ggttctggcc ggaccgccga atcgcccgct ggacggaagt gatcggcatg gaacacattc
    52561 gtgacgtgca ggcgcaaaaa cgcggcatcc tgttagttgg catccatttt ctgacactgg
    52621 agctgggcgc gcggcagttt ggtatgcagg aaccggggat tggcgtttac cgcccgaacg
    52681 ataatccact gattgactgg ctacaaacct ggggccgttt gcgctcaaat aaatcgatgc
    52741 tcgaccgcaa agatttaaaa ggcatgatta aagccctgaa aaaaggcgaa gtggtctggt
    52801 acgcaccaga tcatgattac ggcccgcgct caagcgtttt cgtcccgtta tttgccgttg
    52861 agcaggctgc gaccacgacc ggaacctgga tgctggcacg gatgtccggc gcatgtctgg
    52921 tgcccttcgt tccacgccgt aagccagatg gcaaagggta tcaattgatt atgctgccac
    52981 cagagtgttc tccgccactg gatgatgccg aaactaccgc cgcgtggatg aacaaagtgg
    53041 tcgaaaaatg catcatgatg gcaccagagc agtatatgtg gttacaccgt cgctttaaaa
    53101 cacgcccgga aggcgttcct tcacgctatt aaatctccca tgccggatgc ttcagaatgg
    53161 catccggcat taccacagca aatccccctg atttagcgat aaaagctctc tggattgcgc
    53221 cccctggaag tcgggcgcat aattagtgtg tttatctttt cttcttatgt tcaccgcgcc
    53281 tggcgcacca acagcggatt gctatgtcac cctgtgaaaa tgacacccct ataaactgga
    53341 aacgaaacct gatcgtcgcc tggctaggct gttttcttac cggggccgcc ttcagtctgg
    53401 taatgccctt cttacccctc tacgttgagc agcttggtgt taccggccac tccgccctga
    53461 atatgtggtc cggtattgtc ttcagtataa tgggttccta aacacatcat tttaggtatt
    53521 gaatatgaga cgggcaatca gctacatcag attttcaagt gaacgacaac tgaaagggga
    53581 ctctgtacgc cgacaatcta agctggttac tgactggctt gataagaacc ctgagtttta
    53641 cttggattca tccttgagct ttaaagattt ggggaaaagt gcttttagtg gtaagcacct
    53701 gaaaggtggt ctaggtgact ttttaaccgc tatcgagaaa gggcttgtta aagctggtga
    53761 tacgctacta atcgagagtt tagaccgtct gtcacgtcag gatattgata ttgcttctga
    53821 gctattacgc cgtatcttaa gggctggtgt agatgtagtc acactgagcg atggagaaca
    53881 ttacacacga gaaagtttaa aagacccgtt agccctgatt aaaagcattc tgataatgca
    53941 gcgggcgcat gaggaaagtt tgagaaagtc tgagcgtgtc caagctgcat ggaaccgtaa
    54001 aaaagaactc atctccgaag gtatcaaggt atctaggcgt tgtcctgcat ggttaaggct
    54061 gaatgatgac cgtcgaacat tcacaatcat tcctgataaa gtggaagttg ttaaacgagc
    54121 atttgacctg agattacagg gtttaagctt ttgggctatc accagaactt taaacgatga
    54181 aggtcacttg tcattgaatc aatacacacc caaacaaaag gggtggtctg atactgctgt
    54241 aaagaaactg ctcagaaatc gtgctgtgat tggatgtttc acacctgctg gacgtgaaga
    54301 agttcagggt tactaccccg caataatcag tgagtcacta ttctatcgag tacagcaact
    54361 gaacacaggg caatatggta gggcttctgt atcaagtaat ccgctatcag tcaacttgtt
    54421 caggggtatc atcaa
//
LOCUS       DAZSNM010000005        53211 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZSNM010000005 DAZSNM010000000
VERSION     DAZSNM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53211
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.005"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(11..322)
                     /locus_tag="ACHW42_000224"
     CDS             complement(11..322)
                     /locus_tag="ACHW42_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR8942211.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="ACHW42_000225"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHW42_000225"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="ACHW42_000226"
     CDS             701..1054
                     /locus_tag="ACHW42_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR8942212.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHW42_000227"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHW42_000227"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFR8942213.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="ACHW42_000228"
     CDS             complement(2810..3280)
                     /locus_tag="ACHW42_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR8942214.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHW42_000229"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHW42_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /locus_tag="ACHW42_000230"
     CDS             complement(4119..4337)
                     /locus_tag="ACHW42_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR8942215.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHW42_000231"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHW42_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR8942216.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /gene="acrB"
                     /locus_tag="ACHW42_000232"
     CDS             complement(5283..8432)
                     /gene="acrB"
                     /locus_tag="ACHW42_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR8942217.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="ACHW42_000233"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="ACHW42_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR8942218.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="ACHW42_000234"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="ACHW42_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR8942219.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="ACHW42_000235"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="ACHW42_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR8942220.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="ACHW42_000236"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="ACHW42_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR8942221.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="ACHW42_000237"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="ACHW42_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR8942222.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="ACHW42_000238"
     CDS             14911..15288
                     /locus_tag="ACHW42_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR8942223.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="ACHW42_000239"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="ACHW42_000239"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR8942224.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="ACHW42_000240"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="ACHW42_000240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR8942225.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="ACHW42_000240"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="ACHW42_000241"
     CDS             18099..18428
                     /locus_tag="ACHW42_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR8942226.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="ACHW42_000242"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="ACHW42_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR8942227.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="ACHW42_000243"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="ACHW42_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR8942228.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="ACHW42_000244"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="ACHW42_000244"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR8942229.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="ACHW42_000245"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="ACHW42_000245"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR8942230.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="ACHW42_000246"
                     /pseudo
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="ACHW42_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="ACHW42_000247"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="ACHW42_000247"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR8942231.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /gene="ybaL"
                     /locus_tag="ACHW42_000248"
     CDS             complement(25481..27157)
                     /gene="ybaL"
                     /locus_tag="ACHW42_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR8942232.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="ACHW42_000249"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="ACHW42_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR8942233.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="ACHW42_000250"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="ACHW42_000250"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR8942234.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="ACHW42_000251"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="ACHW42_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR8942235.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="ACHW42_000252"
     CDS             complement(31205..31999)
                     /locus_tag="ACHW42_000252"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR8942236.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="ACHW42_000253"
     CDS             32137..32478
                     /locus_tag="ACHW42_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR8942237.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="ACHW42_000254"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="ACHW42_000254"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR8942238.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="ACHW42_000255"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="ACHW42_000255"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR8942239.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="ACHW42_000256"
     CDS             36394..37686
                     /locus_tag="ACHW42_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR8942240.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37717..37814
                     /locus_tag="ACHW42_000257"
                     /pseudo
     CDS             37717..37814
                     /locus_tag="ACHW42_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            37811..38218
                     /gene="cueR"
                     /locus_tag="ACHW42_000258"
     CDS             37811..38218
                     /gene="cueR"
                     /locus_tag="ACHW42_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR8942241.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38219..38677)
                     /locus_tag="ACHW42_000259"
     CDS             complement(38219..38677)
                     /locus_tag="ACHW42_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR8942242.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="ACHW42_000260"
     CDS             complement(38674..39591)
                     /locus_tag="ACHW42_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR8942243.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="ACHW42_000261"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="ACHW42_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR8942244.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="ACHW42_000262"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="ACHW42_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR8942245.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="ACHW42_000263"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="ACHW42_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR8942246.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="ACHW42_000264"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="ACHW42_000264"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR8942247.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="ACHW42_000265"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="ACHW42_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /gene="ybbA"
                     /locus_tag="ACHW42_000266"
     CDS             43550..44236
                     /gene="ybbA"
                     /locus_tag="ACHW42_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR8942248.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46648
                     /gene="ybbP"
                     /locus_tag="ACHW42_000267"
                     /pseudo
     CDS             44233..46648
                     /gene="ybbP"
                     /locus_tag="ACHW42_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
     gene            complement(46866..47021)
                     /gene="dinQ"
                     /locus_tag="ACHW42_000268"
     CDS             complement(46866..47021)
                     /gene="dinQ"
                     /locus_tag="ACHW42_000268"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR8942249.1"
                     /translation="MKKIIVIGNVRVSCDCLSPLREQRMKTFLDKVLIVLKALIALLE
                     LIRQFIE"
     gene            complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="ACHW42_000269"
     CDS             complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="ACHW42_000269"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR8942250.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48524..49450)
                     /gene="allS"
                     /locus_tag="ACHW42_000270"
     CDS             complement(48524..49450)
                     /gene="allS"
                     /locus_tag="ACHW42_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFR8942251.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49680..50162
                     /gene="allA"
                     /locus_tag="ACHW42_000271"
     CDS             49680..50162
                     /gene="allA"
                     /locus_tag="ACHW42_000271"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFR8942252.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50240..51055
                     /gene="allR"
                     /locus_tag="ACHW42_000272"
     CDS             50240..51055
                     /gene="allR"
                     /locus_tag="ACHW42_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFR8942253.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51145..52926
                     /gene="gcl"
                     /locus_tag="ACHW42_000273"
     CDS             51145..52926
                     /gene="gcl"
                     /locus_tag="ACHW42_000273"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFR8942254.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52939..>53211
                     /locus_tag="ACHW42_000274"
     CDS             52939..>53211
                     /locus_tag="ACHW42_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR8942255.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12830 a        13690 c        14016 g        12675 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcacagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacctgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctagtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggctcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgttgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tatggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgactat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct atggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggcgttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcggtctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg tttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg
    47341 ccgtttcaga ataccgccca tcactgcgcc ttaacccata ccgccacttc cgcccactca
    47401 ccgcgaaaga caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa
    47461 tattctttaa gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt
    47521 tgcgttgtca gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc
    47581 cgacgcttaa tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt
    47641 tcgtcgccat aagcgtgggt aaaatcatga tgcatacgca agaaatactc ttcattcagg
    47701 cgttcaagac ggatctcaaa cggatcttct accaccgcaa tcgccgcctg agtcattcgc
    47761 tcgcgcagac attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc
    47821 cacaggcgca aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca
    47881 aaactcgcct ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc
    47941 gccagccctt ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg
    48001 ccggtacaac cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc
    48061 gcggtctggc gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca
    48121 tgcagccagc gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct
    48181 tgcggatttt gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc
    48241 gccaccagtt tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg
    48301 ccaacggagg cgcgttcatc gttatccatt aacggcagat tgatagcggc gggcattgcg
    48361 ccttgctcaa actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg
    48421 gcacgatagt cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc
    48481 ttgcgcgcta ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtggggtgc
    48541 cgaaaatttc taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat
    48601 cttctaccgc tttgcagctg ccaaatttgc gccatgccag actcaatggc gaaggagggc
    48661 gcatcgttgg gattacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg
    48721 attttggcaa aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat
    48781 cgggaacaat aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc
    48841 cggcgctgtc ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct
    48901 cttcaacgtt cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta
    48961 agggatcaag actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg
    49021 aaaaaccttc gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa
    49081 actgggtaaa ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gcctgggggt
    49141 tatagagcag gttgttgatg acaatattca cctggcgttc cacgccatca ttcacctgtt
    49201 gcagctcgct gggcatactt tccagccagc tcagccagtc tctggcctgg gaaagtagat
    49261 gctcgccagc cgctgtcaac gttacgctac gagtcgtacg gaaaaacagc gctactccgg
    49321 tattctcttc cagaagttta atgcgataac tgatcgtcgc cgtggtttta cataatcgtt
    49381 ctgccgcttt tgaaaaactt cctgtttcag caaccgcaat gaaagtccgc aaggtttctg
    49441 gatcgaacat cttcaggtat ccccttttaa atccgcaagt tgcgtgattt tcttatcctc
    49501 tgatttatca gtatttttac atgataatcc tgttcaattt gtggactaaa tctagttttg
    49561 gaaaaacatt ccaacttttg tattaatgtt gttctcttaa ggttttagat tgcctgttat
    49621 tgaaaccaag ctgaccggtc ggcggtggtt gaacggaatt atgttacaag gacaaaaaga
    49681 tgaaacttca ggtattaccg ttaagtcagg aagcctttag tgcttatggc gacgtaatcg
    49741 aaacgcagca acgggatttt ttccatatta acaatggcct ggtggagcgt taccacgatt
    49801 tggcgctggt tgagattctt gagcaagacc gtacgcttat cagcattaac cgcgcgcaac
    49861 cggcgaatct gccgctgacc attcacgaac tcgaacgtca tccgctgggc attcaggcct
    49921 ttatcccaat gaaaggtgaa gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag
    49981 acctgtcaac gctgcgggcg tttatcacca acggtgaaca gggggtgaat taccatcgta
    50041 acgtctggca tcacccactt ttcgcctggc agcgcgtcac cgattttctg accatcgatc
    50101 gcggcggcag tgacaactgt gatgttgaaa gtattcctga acaggaactc tgttttgcgt
    50161 gacgcctgca accgacttgc ataagataaa ctaattgttc attgtttatg ctcacttgta
    50221 ggtcggagtt aacgtaggta tgacggaagt tagacggcgc ggcaggccag gacaggcgga
    50281 gcctgtggca cagaagggcg cacaggcgtt agagcgggga attgcgattc tgcaatatct
    50341 ggaaaaaagt gggggaagtt cgtcggttag cgatatttct ctcaatctgg atttgccgct
    50401 ctccacgacc tttcgcttgc tgaaggtttt acaagcagcg gattttgtct atcaggacag
    50461 tcaattaggc tggtggcata taggattagg tgtctttaac gtcggtgcgg cgtacatcca
    50521 taaccgcgat gtcctctccg tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg
    50581 cgaaacggtc aatgtcgcga tccgtaacgg caatgaagcg gtattaattg gtcagttaga
    50641 gtgtaaatcg atggtcagga tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc
    50701 cggtgcgggc aaagcgctgc tttatccgct ggcggaagag gagttgatga gcatcattct
    50761 gcaaaccggt ttgcagcagt ttacgccaac tacgcttgtg gatatgccca ccttgctgaa
    50821 ggacctggaa caagcgcgtg aactgggcta taccgtagat aaagaagagc atgttgtagg
    50881 tctgaattgc atagcttcag caatttacga tgatgtcggt agagttgttg ccgctatctc
    50941 catctccggg ccttcatcaa gactgacaga agatcgtttt gtcagtcagg gtgagctggt
    51001 cagagacacc gcccgcgata tcagcacggc gttgggactg aaagcacatc catagtgtct
    51061 gtcgcatccc gctctgcgga gcgggttttt ttgacaaaat ttgaaagttg gaaaattttt
    51121 ccaactaata gaggtaggaa taaaatggca aaaatgagag ccgttgacgc ggcaatgtat
    51181 gtgctggaga aagaaggtat caccaccgcc ttcggcgttc cgggagctgc aattaatccg
    51241 ttctactcag cgatgcgtaa gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa
    51301 ggtgtttcgc acatggcgga aggttatacc cgcgcaacgg cagggaatat cggcgtatgt
    51361 ctggggactt ccggtcctgc gggcacggac atgatcaccg cgctctattc cgcttctgct
    51421 gattccattc ctattctgtg cattaccggc caggcaccgc gcgcccgtct gcataaagaa
    51481 gattttcagg ccgtagatat tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca
    51541 gttcgtgaag cggcgctggt gcctcgcgtg ctgcaacagg catttcacct gatgcgttct
    51601 ggtcgtccgg gtccggtact ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag
    51661 tttgatcctg acatgtacga accgctgccg gtctacaaac ctgctgccag ccgtatgcag
    51721 atcgaaaaag ctgtagaaat gttaatccag gccgaacgtc cggtgattgt tgccgggggc
    51781 ggggtaatca atgctgacgc agctgcactg ttacaacagt ttgctgaact gaccagcgtt
    51841 ccggtgatcc caacgctgat gggctggggc tgtatcccgg acgatcatga actgatggcc
    51901 gggatggtcg gtctgcaaac cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac
    51961 atggtgtttg gtatcggtaa ccgttttgct aaccgtcata ccggttcggt agagaaatac
    52021 accgaagggc gcaaaatcgt tcatattgat attgagccga cgcaaattgg tcgcgtgctg
    52081 tgtccggatc tcggcattgt ctctgatgct aaagcggcgc tgacactgct ggttgaagtg
    52141 gcgcaggaga tgcaaaaagc gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc
    52201 cagcagcgta aacgcacttt gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg
    52261 cagcgcgtgt atgaagagat gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact
    52321 attggtctgt cacaaatcgc tgcggcacaa atgctgcatg tctttaaaga ccgccactgg
    52381 atcaactgtg gtcaggctgg tccgttaggc tggacgattc cggcggcgct ggggggttgt
    52441 gccgctgatc cgaaacgcaa tgtggtggcg atttctggcg actttgactt ccagttcctg
    52501 attgaagagt tagccgttgg cgcgcagttc aacattccgt acatccatgt gctggtgaat
    52561 aacgcctatc tcggcctgat tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg
    52621 caactcgctt tcgagaatat caactccagt gaagtgaatg gctacggtgt tgaccacgta
    52681 aaagtagcgg aaggtttagg ttgtaaagct attcgggtct tcaaaccgga agatattgcg
    52741 ccagcctttg aacaggcgaa agccttaatg gcgcaatatc gggtaccggt agtcgtggaa
    52801 gttattctcg agcgtgtgac caatatttcg atgggcagcg aactggataa cgtcatggaa
    52861 tttgaagata tcgccgataa cgcagcggat gcaccgactg aaacctgctt catgcactat
    52921 gaataaggga gataaatgat gttacgtttc tctgctaatt tatcgatgtt atttggtgaa
    52981 tatgattttc tcgcccgttt tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt
    53041 atgtttcctt atgactacga cattgaagaa ttaaaacagg tactggcgag taataaactc
    53101 gaacatacgc tgcacaattt accggcaggt gactgggcgg cgggcgagcg cggtattgcc
    53161 tgtattcctg gccgtgaaga agagtttcgg gatggcgtag cagcagcgat t
//
LOCUS       DAZSNM010000006        50729 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZSNM010000006 DAZSNM010000000
VERSION     DAZSNM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50729
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.006"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1958)
                     /gene="mobF"
                     /locus_tag="ACHW42_000275"
     CDS             complement(<1..1958)
                     /gene="mobF"
                     /locus_tag="ACHW42_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HFR8942256.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPG"
     gene            complement(1958..4165)
                     /gene="traD"
                     /locus_tag="ACHW42_000276"
     CDS             complement(1958..4165)
                     /gene="traD"
                     /locus_tag="ACHW42_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFR8942257.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIAEFAAGEIGEKEHLKASLQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQAEQPQQPQQ
                     PQQPQQPQQPQQPQQSQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPP
                     GISESGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(4216..4953)
                     /locus_tag="ACHW42_000277"
     CDS             complement(4216..4953)
                     /locus_tag="ACHW42_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942258.1"
                     /translation="MTSDNIAEYIGENNQFTEKPATEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            complement(5156..5887)
                     /gene="traT"
                     /locus_tag="ACHW42_000278"
     CDS             complement(5156..5887)
                     /gene="traT"
                     /locus_tag="ACHW42_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFR8942259.1"
                     /translation="MKTKKMMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDAAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(5901..6410)
                     /gene="traS"
                     /locus_tag="ACHW42_000279"
     CDS             complement(5901..6410)
                     /gene="traS"
                     /locus_tag="ACHW42_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFR8942260.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGVYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSITLFVIISFIYTIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            complement(6407..9232)
                     /gene="traG"
                     /locus_tag="ACHW42_000280"
     CDS             complement(6407..9232)
                     /gene="traG"
                     /locus_tag="ACHW42_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFR8942261.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTGSKFSYGGY
                     ADAHAEWGIKPGILGVGGGVGVRGGFKGSLDRTDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEIAERRRAMAWFFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            complement(9229..10602)
                     /gene="traH"
                     /locus_tag="ACHW42_000281"
     CDS             complement(9229..10602)
                     /gene="traH"
                     /locus_tag="ACHW42_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFR8942262.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMNYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            complement(10589..10981)
                     /gene="trbF"
                     /locus_tag="ACHW42_000282"
     CDS             complement(10589..10981)
                     /gene="trbF"
                     /locus_tag="ACHW42_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFR8942263.1"
                     /translation="MKEKINDTEPGIKQIEREIERGCDNAKKYFWLFVVFFAAGLIVR
                     NVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLAAASLSPLCEII
                     FGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            complement(10962..11309)
                     /gene="trbJ"
                     /locus_tag="ACHW42_000283"
     CDS             complement(10962..11309)
                     /gene="trbJ"
                     /locus_tag="ACHW42_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFR8942264.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTCLHESS
                     EISEGTYDERKNK"
     gene            complement(11239..11784)
                     /gene="trbB"
                     /locus_tag="ACHW42_000284"
     CDS             complement(11239..11784)
                     /gene="trbB"
                     /locus_tag="ACHW42_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFR8942265.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            complement(11771..12055)
                     /gene="traQ"
                     /locus_tag="ACHW42_000285"
     CDS             complement(11771..12055)
                     /gene="traQ"
                     /locus_tag="ACHW42_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFR8942266.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            12136..12471
                     /locus_tag="ACHW42_000286"
     CDS             12136..12471
                     /locus_tag="ACHW42_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFR8942267.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYIYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            complement(12452..12793)
                     /gene="trbA"
                     /locus_tag="ACHW42_000287"
     CDS             complement(12452..12793)
                     /gene="trbA"
                     /locus_tag="ACHW42_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFR8942268.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(12807..13550)
                     /gene="traF"
                     /locus_tag="ACHW42_000288"
     CDS             complement(12807..13550)
                     /gene="traF"
                     /locus_tag="ACHW42_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFR8942269.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(13543..13800)
                     /locus_tag="ACHW42_000289"
     CDS             complement(13543..13800)
                     /locus_tag="ACHW42_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFR8942270.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGIL
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(13827..15731)
                     /gene="traN"
                     /locus_tag="ACHW42_000290"
     CDS             complement(13827..15731)
                     /gene="traN"
                     /locus_tag="ACHW42_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFR8942271.1"
                     /translation="MKEKINTFFRSLNFNYSVKPCNLCAIVFFLSFSVFADSNSDYRA
                     GSDFAHQIKGQGSSSIQGFKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWA
                     TGETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEF
                     TNYTCERDTMVEEYCTRTASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPV
                     TGTVESASLSVYAAFFFLNSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLT
                     GSGCTANGNCLPHGNGDRKVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNK
                     ADGVLTGTECSEPGGTKTGVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPAC
                     TLASRQCAFYSDEGTCLHEYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDF
                     GEAVSQLAALAAAGKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLA
                     KCNSEEKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNG
                     QLRIGFGSAKHPDCRGITVDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQ
                     IADQLKQAGQ"
     gene            complement(15967..16347)
                     /locus_tag="ACHW42_000291"
     CDS             complement(15967..16347)
                     /locus_tag="ACHW42_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942272.1"
                     /translation="MTLPAGYYQIDPEIRALVAAMNIHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLAWGWSVKGAFNSKFQLCFRLQPDTPHYWY
                     NRYCRHSLCADFRTLISLLKSLSE"
     gene            complement(16344..16982)
                     /gene="trbC"
                     /locus_tag="ACHW42_000292"
     CDS             complement(16344..16982)
                     /gene="trbC"
                     /locus_tag="ACHW42_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFR8942273.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVARALLNNPGDKQK"
     gene            complement(16991..17296)
                     /locus_tag="ACHW42_000293"
     CDS             complement(16991..17296)
                     /locus_tag="ACHW42_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942274.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(17326..18318)
                     /gene="traU"
                     /locus_tag="ACHW42_000294"
     CDS             complement(17326..18318)
                     /gene="traU"
                     /locus_tag="ACHW42_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFR8942275.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(18315..18947)
                     /gene="traW"
                     /locus_tag="ACHW42_000295"
     CDS             complement(18315..18947)
                     /gene="traW"
                     /locus_tag="ACHW42_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFR8942276.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(18944..19330)
                     /gene="trbI"
                     /locus_tag="ACHW42_000296"
     CDS             complement(18944..19330)
                     /gene="trbI"
                     /locus_tag="ACHW42_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR8942277.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(19327..21957)
                     /gene="traC"
                     /locus_tag="ACHW42_000297"
     CDS             complement(19327..21957)
                     /gene="traC"
                     /locus_tag="ACHW42_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFR8942278.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(22083..22430)
                     /locus_tag="ACHW42_000298"
     CDS             complement(22083..22430)
                     /locus_tag="ACHW42_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942279.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(22458..22676)
                     /locus_tag="ACHW42_000299"
     CDS             complement(22458..22676)
                     /locus_tag="ACHW42_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942280.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPFNSVMETLNGGGQ"
     gene            complement(22775..23248)
                     /locus_tag="ACHW42_000300"
     CDS             complement(22775..23248)
                     /locus_tag="ACHW42_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942281.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFAEESKVC"
     gene            complement(23241..23654)
                     /locus_tag="ACHW42_000301"
     CDS             complement(23241..23654)
                     /locus_tag="ACHW42_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942282.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(23647..23868)
                     /gene="traR"
                     /locus_tag="ACHW42_000302"
     CDS             complement(23647..23868)
                     /gene="traR"
                     /locus_tag="ACHW42_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFR8942283.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(24003..24518)
                     /gene="traV"
                     /locus_tag="ACHW42_000303"
     CDS             complement(24003..24518)
                     /gene="traV"
                     /locus_tag="ACHW42_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFR8942284.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(24515..24835)
                     /locus_tag="ACHW42_000304"
     CDS             complement(24515..24835)
                     /locus_tag="ACHW42_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFR8942285.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            complement(24822..25412)
                     /gene="traP"
                     /locus_tag="ACHW42_000305"
     CDS             complement(24822..25412)
                     /gene="traP"
                     /locus_tag="ACHW42_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFR8942286.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(25402..26829)
                     /gene="traB"
                     /locus_tag="ACHW42_000306"
     CDS             complement(25402..26829)
                     /gene="traB"
                     /locus_tag="ACHW42_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFR8942287.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGTSKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(26829..27557)
                     /gene="traK"
                     /locus_tag="ACHW42_000307"
     CDS             complement(26829..27557)
                     /gene="traK"
                     /locus_tag="ACHW42_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFR8942288.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(27544..28110)
                     /gene="traE"
                     /locus_tag="ACHW42_000308"
     CDS             complement(27544..28110)
                     /gene="traE"
                     /locus_tag="ACHW42_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFR8942289.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(28132..28443)
                     /gene="traL"
                     /locus_tag="ACHW42_000309"
     CDS             complement(28132..28443)
                     /gene="traL"
                     /locus_tag="ACHW42_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR8942290.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(28458..28817)
                     /gene="traA"
                     /locus_tag="ACHW42_000310"
     CDS             complement(28458..28817)
                     /gene="traA"
                     /locus_tag="ACHW42_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFR8942291.1"
                     /translation="MNTVLSVQGASAPVENKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(28851..29078)
                     /gene="traY"
                     /locus_tag="ACHW42_000311"
     CDS             complement(28851..29078)
                     /gene="traY"
                     /locus_tag="ACHW42_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFR8942292.1"
                     /translation="MIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVIEENESTFKEL"
     gene            complement(29198..29845)
                     /locus_tag="ACHW42_000312"
     CDS             complement(29198..29845)
                     /locus_tag="ACHW42_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TraJ family protein"
                     /protein_id="HFR8942293.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFGCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINSLHCKEM"
     gene            complement(30037..30420)
                     /gene="traM"
                     /locus_tag="ACHW42_000313"
     CDS             complement(30037..30420)
                     /gene="traM"
                     /locus_tag="ACHW42_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFR8942294.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
     gene            30753..31343
                     /locus_tag="ACHW42_000314"
     CDS             30753..31343
                     /locus_tag="ACHW42_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFR8942295.1"
                     /translation="MVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINGIC
                     EAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(31640..32461)
                     /locus_tag="ACHW42_000315"
     CDS             complement(31640..32461)
                     /locus_tag="ACHW42_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR8942296.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(32572..32868)
                     /locus_tag="ACHW42_000316"
     CDS             complement(32572..32868)
                     /locus_tag="ACHW42_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942297.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            33168..33464
                     /locus_tag="ACHW42_000317"
                     /pseudo
     CDS             33168..33464
                     /locus_tag="ACHW42_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(33783..33908)
                     /locus_tag="ACHW42_000318"
     CDS             complement(33783..33908)
                     /locus_tag="ACHW42_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFR8942298.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
     gene            complement(33850..33999)
                     /gene="mok"
                     /locus_tag="ACHW42_000319"
                     /pseudo
     CDS             complement(33850..33999)
                     /gene="mok"
                     /locus_tag="ACHW42_000319"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            complement(34178..34940)
                     /locus_tag="ACHW42_000320"
                     /pseudo
     CDS             complement(34178..34940)
                     /locus_tag="ACHW42_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            complement(34937..35371)
                     /gene="psiB"
                     /locus_tag="ACHW42_000321"
     CDS             complement(34937..35371)
                     /gene="psiB"
                     /locus_tag="ACHW42_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR8942299.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            complement(35426..37384)
                     /locus_tag="ACHW42_000322"
     CDS             complement(35426..37384)
                     /locus_tag="ACHW42_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR8942300.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            complement(37448..37681)
                     /locus_tag="ACHW42_000323"
     CDS             complement(37448..37681)
                     /locus_tag="ACHW42_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFR8942301.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            complement(37737..38258)
                     /locus_tag="ACHW42_000324"
     CDS             complement(37737..38258)
                     /locus_tag="ACHW42_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR8942302.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDKGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADTTKKGSAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            38557..39000
                     /locus_tag="ACHW42_000325"
                     /pseudo
     CDS             38557..39000
                     /locus_tag="ACHW42_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(39086..39649)
                     /locus_tag="ACHW42_000326"
     CDS             complement(39086..39649)
                     /locus_tag="ACHW42_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR8942303.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREVLPCGTFAYTRVPTMIIRLRA"
     gene            complement(39696..41057)
                     /locus_tag="ACHW42_000327"
     CDS             complement(39696..41057)
                     /locus_tag="ACHW42_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFR8942304.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(41109..41339)
                     /locus_tag="ACHW42_000328"
     CDS             complement(41109..41339)
                     /locus_tag="ACHW42_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942305.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPTPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(41563..41682)
                     /locus_tag="ACHW42_000329"
                     /pseudo
     CDS             complement(41563..41682)
                     /locus_tag="ACHW42_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332787.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(42377..42568)
                     /locus_tag="ACHW42_000330"
     CDS             complement(42377..42568)
                     /locus_tag="ACHW42_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942306.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(42565..42987)
                     /locus_tag="ACHW42_000331"
     CDS             complement(42565..42987)
                     /locus_tag="ACHW42_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR8942307.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(43034..43459)
                     /locus_tag="ACHW42_000332"
     CDS             complement(43034..43459)
                     /locus_tag="ACHW42_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR8942308.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(43877..44653)
                     /locus_tag="ACHW42_000333"
     CDS             complement(43877..44653)
                     /locus_tag="ACHW42_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942309.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(44704..45138)
                     /locus_tag="ACHW42_000334"
     CDS             complement(44704..45138)
                     /locus_tag="ACHW42_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR8942310.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(45152..45373)
                     /locus_tag="ACHW42_000335"
     CDS             complement(45152..45373)
                     /locus_tag="ACHW42_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942311.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(45374..46057)
                     /locus_tag="ACHW42_000336"
     CDS             complement(45374..46057)
                     /locus_tag="ACHW42_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR8942312.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(46134..46427)
                     /locus_tag="ACHW42_000337"
     CDS             complement(46134..46427)
                     /locus_tag="ACHW42_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942313.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            46574..47536
                     /gene="parM"
                     /locus_tag="ACHW42_000338"
                     /gene_synonym="stbA"
     CDS             46574..47536
                     /gene="parM"
                     /locus_tag="ACHW42_000338"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR8942314.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            47539..47889
                     /locus_tag="ACHW42_000339"
     CDS             47539..47889
                     /locus_tag="ACHW42_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR8942315.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(48012..48293)
                     /locus_tag="ACHW42_000340"
     CDS             complement(48012..48293)
                     /locus_tag="ACHW42_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942316.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(48421..48655)
                     /locus_tag="ACHW42_000341"
                     /pseudo
     CDS             complement(48421..48655)
                     /locus_tag="ACHW42_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637348.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(50104..50729)
                     /locus_tag="ACHW42_000342"
                     /pseudo
     CDS             complement(50104..50729)
                     /locus_tag="ACHW42_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
BASE COUNT        12240 a        13904 c        12037 g        12548 t
ORIGIN      
        1 cccggtcggt acatatcccg cagataacgg ctccggctgt ccagccagac cggtgacagt
       61 gcagtcatgg tcacctcagg gtgtccgagc acgccctgcg ttttcagctc actgcgaatg
      121 gcctgtgtca gtatggcctg ttcccgtacc ccgctgacct gtgccacgct ctcttctccg
      181 gctttcacgc tggccgcaaa atctccggcc agccgggcat agcggacatt acggtccggt
      241 tcactgatga tggtggccgg tcgctgttct ccgccctgcc agcgatatgt gttcaccccg
      301 gcatccttca tggccatcag cgcactgcct gtaccggttc gctgcccgct gtcggttatc
      361 aggacctgta cgttatgacg tgcggcacca tccagcaggg ttaacgtctc tttcagggag
      421 agtttttcac cctggtcgac gataacggta ctgcccggcg tgaaggccat gccttccagc
      481 agctgacgac gtccggttat cagttcaccg gacagccgtt catcctgctt caggttcatc
      541 tgcgagcgac ggtcagcagc gataatctgc acctcccgcc cctgttcccg ggccatcatg
      601 accagttcag ccacccgctc acgctgcccg gctgcaccgc cctgcccgga cacaatggcc
      661 agcgacggac ggtcctgtgc cagaacactg accgcatcgc tgtaaccagc cgtccggggg
      721 acacttttct ccggatgtac ggtcacccgg ttctgtttca tgatgtcacg actgagtgcc
      781 cggacggaca gctcatcgag cacatgaatt cctgatgtga acagcccctt ctcacggtcg
      841 agggggataa gctgctcacg gctgatggcc tcatcgatac cggcgcgtgc ccgttcaatc
      901 acaccatttt ccggcggcag tatgccgacc gtcctggcca gcacgtccgt atacgtgaac
      961 tgcacttttc gttcacttaa tccggcaatc gcctgtgtca ccgcctgctg cacatccggc
     1021 ccgtcctgtg aagcaggccc gggcgcctgc gtgcggatct ccgcacgctg atccgccgcg
     1081 tcacgatatg cccggatgtc gaacccggtt tccttcagcg tctgcatcca ttcagccatt
     1141 ctgatttccg gatcgacgtg ctgtttggat tttcgcgtat ccagtgccgc cacatcccgg
     1201 gatttcagcg aggcgtcttc accgacggcc tccctgatag cctgtgaacg actggaaaag
     1261 gcctccaccg gtacgcccgg catctcccac ataccgtgct taccgaccac ttcggtttca
     1321 taacccagcg cctcaacgcg ctgacgtaac tcactctgat atattttccc gaacgcaatc
     1381 cggttcgcga gcacgttctc ggaaaatccg gttttcccca ctttgtcact gctcagtgtc
     1441 ttccactcgc cgttatgctg cgtgacatta gccaccacca catgcgtgtg taactgtggg
     1501 tcctgatcgc gactggtgtc atggttaaac agtgccatca ccagattacc ggtcagcacc
     1561 gtttctgact gtccgtccgt catcacccgt gtggaagcca gcgcctccac ctgacggaca
     1621 gcaaaatcca cggcctggtt atgtgcatca ataagacgtt tatccccccc gagcatggcc
     1681 atcatggaga cacttttggg ggcggagaag gtcaggtcgt agccgggacg atgcttatta
     1741 ctgccatcct gcatgcggct taaatccgcc ccgtccggca gcctgccctc cagaagacgg
     1801 gtaaaaacat ccttatcgac gctgccctgc agcccaagct gttcagcccc ctgaccggcc
     1861 cagcgttctc ccatgctgcc cagcacatag taattatcct tgtcggtgta atagtttccg
     1921 gcactgcctg ccgatttaac aacgctgaaa ctcaacatca gaaatcatct cccggctcaa
     1981 catcctcccc gcgctcccgg tgcacattaa tgttcacctc ttcacgacgc tgcatatgct
     2041 gctggatgtc cggatgattt tcctgttgcc atgcctcata agcggccata tccaccactt
     2101 caccggattc actgatcccg ggtggcagtt gctgttccat ctcttcttcc ggtttcattt
     2161 tcagctcctg ctcgatcccc cctgccggaa cactcacacc tgcatctgac ttcttatcat
     2221 tgatgacaga agacactggc tgttgtgact gttgtggctg ttgtggctgt tgtggctgtt
     2281 gtggctgttg tggctgttgt ggctgttgtg gctgttcagc ctgagtcacg tcttctccgg
     2341 aggcaacctc cggttcgaag aggctggcca tctggcgacc ttctgcttcc cttgcagcaa
     2401 gtacggcact cagacggttc tccatttccg ggttgatgtc acgcggaata aactccggag
     2461 cgaccttcgg tcgtgtctga tatttcagtg agagttttac tgccggatac ggtccgggca
     2521 gagtgacata acaggtcaga tccggcagag actgaatgtc ggaataactg accagcgtct
     2581 ggcgctccat ctccttaccg gtcgataccc cgtcacgcac ggggtcagca ccgtaggaat
     2641 actgcagact ggctttcagg tgctctttct caccaatttc accggcagca aactctgcaa
     2701 tctgatggct cggggagcgg aaaaaggcac gggtattcag cacatcaaac agcgtggccg
     2761 ccgctttctc accgtagata tcttccagct gggcatagga ctggatacca aacacataac
     2821 agcctccgaa cttacgggct tccggcagga tctccaccag gtccggcagt ttgtgtaacg
     2881 tgggtaactc atcacagaaa aaccacacac gacggttacg gttttctccc attgccagca
     2941 gaccacgaat ggcaatggac agccacatgg aaaccaccgg cttcagggag gcatgagtgt
     3001 cggcatttga cgagataaac agccagccgt ttttctgatc ttcccggaca ccccgcatcc
     3061 agtcacgtat ggtgaagggt tcaccgttat gctcaatccc ctgcaggtaa cggatggctt
     3121 tcacgtagtt ggtcagcaca gcacggatgg aaatcgccgt tttctcaatt ttctcttcca
     3181 ccaggttggc cgccggtgaa ttacgcagga aggtacgcag tttttcgatt ttgatggaaa
     3241 gcagtgtgtc caccagtttg ctgtagctgc ggttggggtc attacgcatc agatacgccg
     3301 cttccgcaaa aatggtacgt cctgatccct gccagaacgg gtcctctttc gttcccatcg
     3361 ggatcagcgt gttcgcgaca ttatcaaaat ccggctgtgt caggcactcc ttccacaaat
     3421 cccaggcggc acagcgggca tccagcggat tcaggatctt atcgatggag ggatcatagt
     3481 aacttttaac aaactcacac gaacggtcat aaatcaccac catgtcgcca cgtttacggg
     3541 catagttggc aagacgtcga ataacctctg acttacccgt cgacactgtt ccgtgcagac
     3601 agaagttctg gatttcggaa tcccggataa tcggcagatc gccaatccgg atatcggagt
     3661 ccttgccgtc ttttttcagc atccgggcaa cgtctttcgg attgtctgtc agctgacgac
     3721 cacctgtgac ttcattttcg ctctgttgtt taccctgacg ccccagaatc caggagacaa
     3781 caaagaaggt aatcaggcaa ataaccagtg ccacaactgt agccagaacg aatgcggacc
     3841 atagctgctc tccgcaccag atcatatatt tatcatgcag tacctgagca gcgttcatcc
     3901 ggaaggtttt gccgtaatac tggatctcat agaccggctg ggattttatt aaatcccgca
     3961 tgccttccag tgacgtacac caccagtaaa tacagccgtt gataaacgtc tgccagctta
     4021 tttttaccca taagaccaga ccaatgagga tccagaaaaa aataaacagg cagtaaagca
     4081 tgatattggc gatctggctg aacatgcgga tacgcatgga cgcaatctga ccgccctggg
     4141 tcatatcctt tgcgttaaaa ctcatgatga aactccggga aaatctggaa cagggcagaa
     4201 aaggatgatg tggggttaat tcacagaaac agaccaggtg taccctgtat gccggtggtg
     4261 atgcccgcct gcagtcctgc cggaagagac cttctctgta acaatgtcag tcaggcgcaa
     4321 tacttcagac cagtcactcc catgcggaaa ctggatctga ataaaaaggc gtacccgttc
     4381 acggtgatcc agaactgtgt ccggttcata aaaacggcca ctgtcaggag aaacacagga
     4441 cgttttattg atgagatcat acttatcggc aggagactgc gatgaaatcg cccaggtgat
     4501 attctccgcc tggtggccat caatacgact gctccccggc gtccatgcaa tttcctgcgc
     4561 actcgccacg atgatctccc tggaaacttc agccagcttt ctgggtgtac agaataccgg
     4621 tgccagcaca cacagggaca acaaattaca gctgaccgcc tccggtttaa gcaaattgct
     4681 cccggatttt tcaggaaaag atatttttgt cagtatttca gttaattcct tatgcctgag
     4741 agaattaata aagtacatga taagcatcat gagaaacaat ataacaataa gccctccgat
     4801 aaccactaaa aggtcagtat caatattatt gctgtacaga tagtctttag tggtcactgt
     4861 attatcctgt atcaccgggg ggtattcagt tgcaggcttc tcagtaaatt gattattctc
     4921 accaatatat tcagcgatat tgtctgatgt cataatgact ttctccggaa gatcccggct
     4981 tcgccggcaa cacttcacgg tcgcacaaag aatttgcaca accgctcagt gttttcatat
     5041 ttatttcgga aacgccggga ggtgacaaaa tttattttgt tacatcccgg ggttggacaa
     5101 cgagcaacgc gaggcgtcag caaggaggcc ggtcagatca gcctcctgac gaggttcaga
     5161 gaatatttgc gattgatttg gccagctggt cttcgagaac aggcttcgcc tcttcaaatt
     5221 tcaggttaac cttgttcgca tttgagacca cacgggtctg gtatttatgc tggttacctg
     5281 tttcagtact ggtctgaatt ttcgcaccgg atgtgccctg acgcagggcg gcaacattat
     5341 ccgttgtcac cgttgcctta gtacgctctg caatctgcac atccgtgatc atggtatagt
     5401 tcacatcttc caccatcgca tctgcagcca tacccaccag accagcagca aggcccacac
     5461 cgagtgtggc accggcagaa tttgagttat agccggtaat accggcacct aacgctgcac
     5521 caacagctgc gccttcataa ccacggttca gccatccctg agactcccgc agatccatct
     5581 tatcggcctt cagcacattc gcctgaatcc agtagtaggc tgcatccgga gaagtcacca
     5641 cctgatatcc ttttgctttc acagcatcag caattttgcc ctgcagccca ctcatgtctt
     5701 tatcagacgt gtttttgatc tgcagaaata ccgtgcgttc gctggcgggt ttaagccaga
     5761 tggtctcact catctgagtc ttcacctcaa ggttacgctt cttgattgct gtgctcatcg
     5821 caccacaccc tgaaagggcc agagtggaac tgaccagtgc aaccatcatc attttttttg
     5881 ttttcatcca gaatatcctt ttaactaaca gactcttttt tgaacagacc tattactgag
     5941 actattgcag agagattata acgatttata tctatagtat aaataaagga aataattaca
     6001 aacagagtta ttgataaaaa cccaaataag aacgtatgag caaaggaaac tattgaaaaa
     6061 acaatcacaa acgacaagat ataagttttg ttcttatcat acataaaaga tataactttc
     6121 gactcatctc tgaatttttt agggatggca agatacaaca tcattccatt agtaatggca
     6181 acaaaaagga taaaacccat tacgccacta aaacatatat caaatccagc atctcggata
     6241 tcaactttat ctgccagcaa acgaagaaat gtcggccata ataaaaccca taaaaacaca
     6301 tacacccctg gttttatgac ttgtaataat ggcggaatat taactccctc tacagagagt
     6361 acatccttta gtttctgtgt ctccgttttt atatcatagt gagtgatcat aatggcccct
     6421 tcagtctatc ctttttatca tcaaccattt ttttcacagc ctcttctgat ggctcaggta
     6481 ttatcttttg actttctcgc tcttcattat atttattatt ctgagataat gcctctgttt
     6541 tatgatgatt ttgcagttca gaatactgcc ctcttacaat attttcctcg ccacggatac
     6601 tgttctgagt atctccgata ttgccacgat attctgtgac catattatca acctgatgtt
     6661 ttacatcatt acggatatta ctgtcctgcg ttctttgctc aataatggcc tgatgtccct
     6721 gatggtcagc aataatatcc tgactgccac cacccgaagg tacgctctcc attcctttac
     6781 caatatcgcc acgggattcc cgccaggcat tatcaacacc aggctgtacc tgttcctgca
     6841 caaaaaacca ggccatcgcc cgacgacgtt ctgcaatctc cggcgaactg gtatttgtca
     6901 gaatagcttc tgcatcctgc ggtgcgcgtt tcatcacata ctgtgcaaac tgttgcgaca
     6961 ggtcttcact catctgcccg ctcatgcttt cagtacggga ggccatttcc gcatattcat
     7021 ggctgcgggt catattcgtc gtgtactggt cgtaactctg tttcgctgag ttcagggctg
     7081 cagacaactg gtccacccgg gagtcagcat tattgtcagt atggctgccg gactcactga
     7141 ccttgcgact ggtaaagtaa tcgctggctt ctttaaagtc cttactggct ttagcgtcaa
     7201 tatcatggcg agcatcatgg gtagctctgc tgccactact tgcagaatgt gaatcatcat
     7261 ccgttcgatc aagacttccc ttaaacccgc ctctgacacc aaccccacca ccaacaccaa
     7321 gaattccagg tttaatcccc cattcagcat gagcatctgc atatccgcca tacgagaact
     7381 tagaacctgt tgatgccagt tcctgagtcg cctgctcatt actgatatta tgcgccttcg
     7441 catagctttc cactgcactg cgcatacggc tggccatcat ggagtcctgt gcactcatgg
     7501 tgctgtcagc gccaccggtg acagaatcac tgctcccccg gttagaacca aactgactga
     7561 gcgtgttcca ggcactggca atactgctgc tgaaaccatg cagggcactt tctgctctgt
     7621 tcgacgcctc ccgggccatt tcctgttgcg ccgctgcaat ctgacgcgtt gcattgatac
     7681 cgaccggtaa acgggacatc gctccgcttg catccatcac catattaccg tcacgggtct
     7741 gtgtggcagt tgcgccactg ccggtctggt acatcatctg accaaacgac gtggtgctgt
     7801 tggtgctcca gctgaagccg ttcacgtttt ccgtctgcat gttgccgtag gagtaattac
     7861 cgtcaaccac actgcctgct gcactggccg tcggactgat agaggaagag gcgaagtggc
     7921 tgtacacgct ggaaaaacct gcgccaaggc cttttaccat catccatgac aacgggggga
     7981 tcatggctga aagatacccg gcagtagagg ccaggtcaga gtatttcagc tgtatctgag
     8041 agagttcaga cagcacgacc ggcgcaccat tcatcttcgc atagaatgtc atggcactgt
     8101 tcagaatggc atacaacaac ggccaggtct gcagccacat cagggcaaac acatagcctt
     8161 tcaggacgga cagtgtcagt ttgttgaaga cagctgccag taccagaagc gggaaaatac
     8221 caatggcaat tcccgtcagg atagtctgta ccattggcag gtttcgcatt gtcacatgac
     8281 cgatagagac atgcgccaga cgttgcttct ccatcgatga cgtggtggcc agattcacca
     8341 ggctggcggt gtcaccatta cgggcagcat tactggtgat accctctttc agggcattta
     8401 ttgtgacgtt ctggcgcata atctggctgg cagactgact ggagccgtag aaataactgt
     8461 aactgtcact gaccagttgt ctgaataaca ggtccgggtc cggtctgccg ccaaatatct
     8521 gctgcacata ataatgccag gtcttgcctc ccgtctttgt atcgagattc agcctgtctt
     8581 tcagcgtgac cgacgcatcc ttacaggtta tgaaattatt attgttgtca taaacgcccc
     8641 gtagcgggct gggtcgggaa aaaatcagcg tgtaggggtc agcggaggcc atcagatctt
     8701 ccagcgtgta tttatgattc aggtaaatat cacccagcac gcagttctgg acgtagtcct
     8761 ggaacagatt gatgatttcc ggattccggg acaggaaatc ggtgcttttc acaatcagat
     8821 tcgccccgaa cagcatgcct gtcttgctgt aggtgacgct gtccggttgg gtgaatatca
     8881 tctcgtagct gaccaccatc gcatgcccga tacgggtcgt aagagaaagt ggcatcgcca
     8941 gaccgaccgg aacattatcc acccgatgga ctttgaccag gtcactgtta tcaataatct
     9001 gcaccgatgt gcggacatta accagcaggc tgataagcac aaacacggcc acccagccca
     9061 gcaaatccat cacgttatgc cgctgtaccc acataaaagc cacagcaaca acagacaggg
     9121 tgagcgcgat tttttcaatt gaatcccagg tccgggtact cataaatgca gcaatggcgt
     9181 tcaggttatt ccgcaaccac tcaccaccgg caatcacata aacttcattc acagtgaatt
     9241 ccctccgaag tgatagttgt tctggtaacg gctgagcata cgggcggaaa gctgctgacg
     9301 catgtagttc atctgacgtt cgacaaccag cagcgcatcc tgctggacct gtacctgact
     9361 ctggaaaatg gcaatctgtt gctgtgcctg attcagattc tccagaacat tgtccatcac
     9421 agcctgcgga tagttcccgg tggcaatcat ggcccgggcc tgctgaagca gttcctgaat
     9481 gtactggagc atgatgtcgt agccgatata gtccgtcagc tggtaaacga cgctgttgga
     9541 cacaccgagc atctgcgggt caatcagata tttaaaaacg ggaatggtgg tgctggaaat
     9601 aaacccctgt tcccgggcgt caagcggagt gtcactcact gctttattct gaatactggt
     9661 gagcagtttt gtaatctgtg acttcagggc tttatcccgg gcgatggtca catttgcatc
     9721 cgccaccacc ttcagacact tgtccgaatc gttgcagtgg taaatttttg ccgttccccc
     9781 ctccataatc gcccggagga tatcgcggtc agtggtacgc gccggcaggg gggtaatctc
     9841 gctgtcttcc ccaaaaatca gcgtaccggt gagcgtcatg ataaattctt tcaactcttt
     9901 gttgccgtca aacattctgt tttttttcag cgcctcccat atgatattct ggtttttcag
     9961 cacccgctcc ttgtccttgt cgctggcttt gtcacgaaca gatgaggact gccctccgac
    10021 cgtgcacccc tgacgagatg cggcccagtc ggcgaagata ttgctttcac tgccaatgtc
    10081 ctgacaaact ttctgctgag attcctgcgt ctgagggaac agccccccca caagcccctg
    10141 tgccacctga caggaactga ggttcatact gttaatgtca cttgccattt tctgcaggaa
    10201 gtcttttgcg gtttttattt ccggcaccgt tgtctgcagg gcaaggtcaa aaaagtaacc
    10261 ggcagcattg ctcataatct gcttaacaaa acgctgcaac tgatcgccat taataaaact
    10321 gaacgagccg agataggcgt cgatgccgcc acatccggca ttgatatccg gcagcgtcat
    10381 ggaaatcagc tggatatttt tgacctgggt gcgggcatac agggagccac cataggcata
    10441 cccggcggcc tgcccctgcc agacgcccgg ctgtgtggta ttggaggcga agcccagctt
    10501 gttaaagaac tgattcatat cgctgttcac atccgctgac gctgcgggtg tgaccgtcag
    10561 taaagcggca caaagaacca gaagaggttt aatgcgaagc attattgctg ccctccctta
    10621 atgtgttctc ccggcgcata cggcgtttca gcaggaatat ccggacaccc ccaaatatta
    10681 tttcgcacaa cggcgacaga gaagccgcag caagaaaacc agctgcaagc gcatataaca
    10741 taatcggaat gacatacatc agggtgttcc acatataacg aaaattattt aattccgggt
    10801 cagctttcca cgaatcaata ccggcactga aaaaatcatg cataacgttc cttacaatca
    10861 atccggcagc aaagaaaacc acaaaaagcc agaagtattt ttttgcatta tcacaaccac
    10921 gttcaatctc cctttcaatt tgtttgattc ccggttctgt atcatttatt ttttctttca
    10981 tcataagttc cctctgatat ttctgatgat tcatgcagac aggtcatgcg gctgtaatat
    11041 atttcagaca ccgaaatcca tcccacaatt gcccccacaa aaaacacaac agaaacagaa
    11101 aaggtaagac acagcgaaac gacaatttcc acagggctgg cgggataaaa aagcccctga
    11161 cggataaagg ggatattcag gctgacgatt attcccgtcg cgatgccgga tattccggca
    11221 atgagtaaca ccacctgctt atttcgcacc tttttcctct ccgtatatct gcagaacggt
    11281 atccatccgc gccataaaac tggcggcatc cgttgccccc tgcaaaagcg gtaatgcctc
    11341 aagtgtgttg acgttgacca ggaaggtggt cggtgtggcc accgggatat tcgggaagaa
    11401 ggtctgcatc acgtccggtg gcaccggtaa tgcttccgga aaggcagtat ccccctgacc
    11461 atccagggtg tagggaaaaa cggaaaagcc gtactgctgt gccagctgct tcagtaccgg
    11521 gtcaaactgg tgacagtaag ggcaatgccc ctgcataaac agcaccacct tccagtcggc
    11581 caggttaacc tgcctgccgt tgctgaggcg gaaccagcgg ggagccggct tttctgttct
    11641 ggctgatgtg tctgctgccg gttgtgcagg ctgcacggcc attccctgcg gattccagag
    11701 tcgttcaatt tcatcccggg tggaggcctg cacacctgtg gccatcatca acaaaatgac
    11761 cggcagtaat ttagtgagag acatgtccgc cctcctgctg cccttccatc atggcctgtt
    11821 gcctggcttc cagtgcctca cgctcttcca ggctgacacg tgcaatccat gcatccagca
    11881 cctggtatgc gccgaacagc acaaggatgg ccgcaatcaa ctccgccaga aaaaaagcca
    11941 tccagggttt gctgtaaaca agacgggcga cgatatgaaa ccagacaccc agggaaaata
    12001 cccacattcc cgtaatatca agccggggta aagagaatct gcgtttactt atcatgttaa
    12061 actccggtta ttatttacat gagagtattc atgtctgtaa tattcgcgtt tgtgtgatat
    12121 taatcagagg tacaaatgaa aaagttatct tttaaagaaa aactggaaat agtgagaaac
    12181 atcataagag agtcattatc aggtaatgcg gcttttgctt tactggtgta ttcattattg
    12241 cactccctcc ccattaaaac ctttgctgat ttatttgtga cgatactgtt atttatagcg
    12301 attatcgctg ttgtgatatg gctatttcta atcatctata tttatttttg tgagttattc
    12361 agaagtcact ggatagcagt ctggtttatc ctctggtctg tggtaatcaa cctcactatt
    12421 ggatttggca tttatgacaa attagcacca gtcatttcac taatgaaata ataaaaaacc
    12481 acatcactcc agaccatatg gctgacaaca taatacaaac aaacatcctt ctgaaactgt
    12541 caaacttcgt gataaccggt atggtactga ctgccattga aacaaccggt acggcacact
    12601 gattcataac cgcaccacaa agaaacaaga aattatctgg cagcatacat acacttatga
    12661 catttactac aaagaagaca aacgagctca gcagataaac tcctttatta tttggtcctg
    12721 taaagaaata accaacaacc cccacgataa acaggctggc acctgtaaaa aaacacaaat
    12781 cccctgtatg catatataac ctttggttaa aaattcggtt taaaatcttc agaaacgttc
    12841 aggaactgtt ttgccaggtc gtcctgtgaa ataaagccgt atgataacgg gcgaacactg
    12901 ccctgtttcg ggtcaaccag catcatggcc gggaaatatt tcacaccgag gcgctgcgcc
    12961 tgcccctggt cagtccggga atccggcaac agcggattaa tcacgccatc cacggaaacg
    13021 ggaataacac tcagaccata cgtatcccgg aagccattaa tgacctgcgc cagttgcccg
    13081 tcgatggggt cctgcccccg gtaaaaaaac atgatgccgt agtgttcagc cagtttcgca
    13141 atggcctgtc gctgctgcgc ctggtctgcc gccagctggt tccgtaccgt gccgttgtaa
    13201 tggctgtact gcaggttata gtccagttcg ggatgtgcca gcatggcctt tttagcactc
    13261 atggtgaaaa gcccggcctg ctgagtccag taattctgca gccggaaata tttaacaaaa
    13321 ttatccacgc cgggatacag aatggcttcg tacagcgccc gctttgttgc cgtctgcagc
    13381 gcggccagtt tctgcataat atccggctcc tgacgggggg ctgcaggtac aggtttattt
    13441 tctttttcct tcggatttat tttctcgtta taccactgcc agcctgcatc tttccctgac
    13501 gccggaaaaa taaagcagca gagtaacagt ggcagtaatg ctttattcat cttcattcct
    13561 ttcatcattt tttctttcat catctttctg caggccagtc agggcagaaa aacaaaaata
    13621 cagaataaac aggagcacac cggagtaaag gataacgaaa gcagcagcaa gtatcccgtc
    13681 tgccgtagtt tcttttaccg tatcccacga ccagataatt accggagaaa tgacaatcgc
    13741 tgtaatcgaa agccggataa gaaaatcaac aagacgatta gaagtgaata taactttcat
    13801 catttccccc ggttattttc cataccttac tgtcctgcct gtttcagctg gtcagcaatc
    13861 tgctctttca ctttctgcgt cagaacgcca ctgtccggga ttttctggtt attcatcagg
    13921 tcttcgtaga agttagtgaa atccagtctg tcgaactgaa ttttctgcag ctcatcaacc
    13981 gtaatccccc gacagtcagg atgcttcgca ctgccaaacc cgatacgcaa ctgcccgtta
    14041 cgcccctgct gctggactat ctgcgccagc ttcgaatcaa actggcagta gctgcgcttt
    14101 ttctgcagac agacacccag cactttccgg gagcagaact cgcccacact caccgtcagt
    14161 ttgttgtcct ttgcctttcc cagcgccttt tcttcactgt tgcacttcgc cagccccaca
    14221 tcctgacccc agccgccatc cttacagcag ttactgaagc cggctgcggc cttacggcag
    14281 aatttcgcct cacccgtgaa agccctgaca tccacaccat tcagggcggc aacatcctta
    14341 cctgcggcgg caagcgcagc cagctgtgat actgcttcac caaaatcact gttttttccg
    14401 ctctgtgcct tatcgcattc accgtcaagg cagaagacat caccaccaca gaccatcact
    14461 tttcctgatg ttctggactc gcaggagtac gtggcgtact catgcagaca ggttccctca
    14521 tcggaataaa acgcgcactg acgtgaggcc agcgtacagg ccggattatc gacatatttc
    14581 tgacaggtgc cgttatctgc ggactgagtg acatatttat cccggtatgc ccagcatgcc
    14641 tgtgtgatgt tccagggttt accttccatc actccggttt tcgtaccacc cggctctgaa
    14701 cactcagtgc ctgtcagcac gccatccgct ttattaaacg gacaactctc cacccactcc
    14761 acccggggaa cccattcttt atcacggacc ttcatcctga gtttcagcgt aaaggtggaa
    14821 gcaccactga caagcgattc atagactttc ctgtcaccat tcccgtgcgg gaggcaatta
    14881 ccgttagcag tacagccact accggtcaga acctgcccct gtgtcacctg caaacctgtc
    14941 gccccggaca gggggtaagt atccgactgc ccgtttgcca ggccgacatt aaaggttgta
    15001 ttcatgaagg tataacggga gttcaggaag aaaaaagcgg cgtatacaga caatgatgca
    15061 ctttccactg taccggttac cggagccgtg acactgaaca ccagtttcag cccgttcata
    15121 ctgaaccgga actgtgagtg cggtatcgtc acttcacgat attcatccgt gtatttccag
    15181 tccccggtga tactggcagt ccgtgtgcag tactcctcca ccatcgtgtc ccgctcacag
    15241 gtatagtttg tgaattcact gcggtttatc tcctgcgcac tgcactgctg tccggtattt
    15301 cccacaatgc tgtcagccct gttcaccaca tccctgccgg tctgaatgaa cggtgcatcc
    15361 ggtgacagaa tgtctttcgg cttgttcatg aaggactctg tgatggtttt tccggtttca
    15421 ccggtcgccc attccgtggt gccgtcattt ttcaggccgc catccccgcc ggcagtcacg
    15481 ccaccgtaat attttgtctc gtccggattc gcgttatagc cggggatact ctcctgtggc
    15541 ttaaagccct gaatactgct gcttccctgc cctttgatct ggtgagcaaa atcagaaccg
    15601 gcccggtaat cactgttgct gtcagcaaac acagaaaagc tcagaaaaaa aacaattgca
    15661 caaagattgc acggctttac ggaataatta aaattcagag atctgaaaaa ggtatttatc
    15721 ttttctttca taacctgaac catactggag aatgaagtga tgtcatctca acttcagaac
    15781 tgtgttgatt gtggtaaagc attaagtcga acagccacaa cgtgcgggaa ttgcagctca
    15841 acggaccctt ttggaaaaaa acggcgcagc gatcgctttt accagtggtt tgtagggctg
    15901 tttattacgg ctattattat tgctggcgga ttatggtaca tggggcttct tgacgtaccg
    15961 ggattactac tcagataacg acttcagtaa cgatatcagt gttctgaaat cggcacataa
    16021 cgagtgacga caataccggt tataccagta atgaggggtg tcgggctgaa gcctgaaaca
    16081 gagctgaaac ttactgttaa aagccccctt caccgaccat ccccatgcca gacgtggtat
    16141 tgctgattca gcatcctgcc gcagtcgctg ctcaagaagg gcagccattt ttaccggaca
    16201 ggcaaaggca atataaggag gcagctttgt aacagggaaa ccatgtccct gacaggaagc
    16261 atatgtgcgg aagccgtgaa tgttcatcgc ggccaccagg gcacggatct ccggatcaat
    16321 ctgataatac ccggcaggta aagtcatttt tgtttatctc ccgggttatt cagcaacgcc
    16381 cgagccacct gccggcagtc ccccgtggcg gccactttct ccagcgcctg gccgacccgc
    16441 aggttacctc ggataatgtc gtatccctga ctgcagaaca ccaccagtgc cggtacactg
    16501 cgaatgccgt actgtgaaaa cagcgtcggg tcgatctgaa caccatcagt cgcgccgtct
    16561 ttcaccaggg acagcacggc ttcagcggtg gtcttcaggt cattattcac catgccccgc
    16621 agtgtggcgg gtataccgta gtgccgggtt tcacccagca tccgtttcag cccctcttcg
    16681 ggaatggaaa acgacacaaa atacagggca ccctgccggg gtttcccgtc ctgcgaagcc
    16741 tgctgtttac gaaccagctc atccagaaaa tggttatctg aacgctgaag ggggttttcc
    16801 agcacctgtt tctccgccca ggctttcagc tgatggtcag gtttctctcg cagttgtctg
    16861 cttaaatttt cctgctgctt caggaactgg cggttttcag gtgtgttcac attttctgaa
    16921 gcatgaacag caagatgcat ccctgctgtc agagcaaaaa gtgcaaatat tttccggttc
    16981 atgatatttc tcactttctg ctatttacag gcatgaactc atcagaaaaa agataattga
    17041 gaaaaacagc cataaacgca catgtaaatg tcaccttcag atataatctg acaagtgtca
    17101 cttgttctga tatatctgcc ttcttccatt catgaataat ttcagcgaga aaactgtcag
    17161 gtgcaaaata aaataatata acgggcacca ttacaagacc aaaaggaaat aattttaatg
    17221 ttctgcttat cagcctgagc agcacagcac ttattttcct gaggtgctga atatatttac
    17281 tgtccattgt tgccattttc atttcctctt attcattaaa atgatttaca ggaatacaca
    17341 gttacgttta cgccacatca ggtagccgaa gtttttcttt gtgttgggcg gattttttcc
    17401 cgtctcccag cgcatcacac tgcgcccgaa cggatggcac tgcccgctgt ccggatacat
    17461 attcaccatc tgataacgcc agcgttcttt gggcaggatt ggggacggat attcattaca
    17521 gacggcgtta tttttcccga tggtttccat aatcatgccc tgacggtgta gtttgaacgc
    17581 catgcgttca ctgaccagca gggaggactg caacggactg gactcattac tcacccagcc
    17641 attgaacggg tacatacttc cctgcgaacc ggcacaccag aacagaacat cgagaggcat
    17701 attaaaggcg ctggcaatgg catctgctgc acaggccccc tgtgctatcg gattggcaaa
    17761 gatgacagct tccggattga gaatggtggt caggctgctg tccgtccagg tggggtcaat
    17821 ttcagaaaga taagcgatat ccaggtcacc accttccaga cagcccagcg atgtgatgat
    17881 gttcagccag tacgtcagcg ggtatttgta ccagtgaacg tgatagaacg ccccgttcac
    17941 ctgcttctca tcctttttcg ccgttccctg tgccgtttta ccaaaagccg gcaggctgaa
    18001 gcccaggtta accatgcatc ccggtgaccg ggtgacgtcc gtcagcgcca tcggctccca
    18061 gtaaccaatg gccagcccga tacgcctgaa cagcggcggc ggtgccggac aaatctgaat
    18121 gggcatcgac gggttcgccg tgtcggggac cttaccctga ctgactttga cactgcccag
    18181 cgagagcggg aaaatacagc tccagcagat atccgtgatc gggtttacaa aacgcccctc
    18241 acaggcagaa tccgcagacg ctgcagggac atgaccggcg agaaggaata acatcagcag
    18301 ccacaggctt cgcttcattt tctgccctcc tctgccggaa taaattccac cttcaggaag
    18361 cgatcgccgg gaacggcgct cacccgtgcc gggacctgat caatgccgag gcgctggcag
    18421 agcacaccgt tctggtcaaa gtaaacacgg ctgtccagag acttctgcat ctccgggata
    18481 ctgccctgca cgaggataat tttgctctcc agtgtgggcg gcgtctggcg tttcatccag
    18541 gccacctgcg ccggatcatc gccattgatg aaatacagcg tctggttaaa aggcacatac
    18601 tgcagcggat tcatcacttc accctgacgg gcaaacaccc gcccttcgtt atcccggata
    18661 tccgcagcca gtctgacgga cgggtcaaac aaccggctgc cgtatttctc cgtgcgtccg
    18721 ataccgggca cggcaggggg ccgcagactg ttgcggatca cccgctcctt aaaggcatcc
    18781 attttccggc ccatctcacc ggactgctcc agtgccgtca ggcgctgcat gatcaccgtc
    18841 agcatgtccg gctcctttac cggccacaga tcgccccagg tgccaagatc ggcggcggcc
    18901 acactctgcc cccatatcag cagggcaatc agcccccggc atctcatggt tccgctctca
    18961 ttcgccgggc aatatcctgc tggatttccc gggtgatatc cggagccccc tgtaccacgg
    19021 caggcgacac cagaatgacc gcgtggtgtt tctgctgcca cgcctgcagg ctggcttcca
    19081 gtgccgtgtt aaaacgcgct gaaagggctt ttgactgtgc ttctgacagt tgtttctgac
    19141 tggcgctgtc aaaaaatgca tccacagtct gtttcatgtt gaaggcggca gtcaccggtg
    19201 cgttcagtct gacaatgccg taactgacgg ctgcgttcag taacaccatt gccaggcatc
    19261 ccggcaccgt ccaccaccag tggctgcgtc ggggggctgt aacatcagtc gttttttgcg
    19321 ttgtggtcat gcaacactcc tgtatttctc atgttcttcc agccaggctt ccagcgaagc
    19381 catctccggc cctgacttct tccacgccag ctgccacacc gcctcatgaa tactcaggcc
    19441 ctccctgcgt ttctgctgca caaactcaaa atccgggccg tcagagctgt acatggcgcg
    19501 gcttaacggg tccacaaaca gacggtgcca ggaggaatgg ttttccacct gcagcaggaa
    19561 ggaactgaac cactggtctt tggccgcacc aaacttgcca atcatgtcgc gctgcagtgg
    19621 ctggaactga tccgggaata actggttata tttcgcgaac tccttcgcac tctgtttgag
    19681 gataattttg taggaggagt taccccatgc cgcacgggcc gcactggagg ccttgtcaga
    19741 gtcaaagtcg acgatgttct gtgtgatggt gatataggca ccggtatggc ggcgacaggt
    19801 gcggtaacct ttttgaataa actcaccgac cttgcgattt ttgaagtcca gcagacgcca
    19861 gccttcatca atgacgttca gtttcttgag agtacgcggc gtgcggtaca tcctgttctc
    19921 gatgtagata atcagggaga acatcaccgc aaccagcagt gacggacggt cttccagtcc
    19981 gcccagctcc agcaccacca ttttcgcatc atcccgcagg gacggctcat cggagttaaa
    20041 atactggccg taagtgccgt tggcagtgta ctggtcaagc agcacaatca tttcgtccag
    20101 acggctgcgg atggtcggtg acccggcata ctgctcgctg tcactggcgt ttttcaggaa
    20161 atccaccacg tcatcaatac gtgccctgtt ctctttggcc agccaggagg ccctgaccgc
    20221 ctgcagcagc agaccttcat gcacttcatc caggttaccg ttggggctgg ccatcactga
    20281 caactggtca cggacacgct ccgctgactg gtcaatatcg gtgatgttcg caaacgggtt
    20341 aaaacgcagg gtttcaccgt ccagatatac gccccccatg ttctcacaca gggacttgta
    20401 gccatccccc atgtcgaaca ccacggcaaa gcccccggag tccagcacgc tgcggataag
    20461 tggctgtatc agtccggttt taccggcccc ggaggtgcca cagaccgcca tgttgtagtt
    20521 ggtgttattc atccccttaa agaagatatc gataaacgcc agctggttac ggtaggtggg
    20581 cgccagcaga cctgccggtg tcagggggtt atccgccact aacggcatca ggttggccac
    20641 attaaagctc tctgcgcgct gaaccacacc ggcctctttc agctgtttaa acagcccttt
    20701 cccggccata aagggcaggc aggtcaggaa attgcgcatg tggttaaagc gcggtgaaat
    20761 cagctcaaaa ccgtttttac ggaagctgtt caggatgtcc tgctccactt ccagtgccgt
    20821 ttcattattg tccttgcaga aggctgtgat gttgaggaag taggacacga cagaagactg
    20881 gccggagccc agccgctggc gcagttcccc ccactccttc gcctctttct ccacggacgg
    20941 aaaccatttg gcatatgagg tcttcgactt cttctccagg tccatgtact tcaggttggc
    21001 ttcgctgtgg gttttcacct ggtcttccac caccagagtc agcgtcagga tgaacgggca
    21061 ggaaatggac atttccgggt tcagcagatt gctgtagttg tcggccatgt tccacaggaa
    21121 ggcgatttcc gggttacggg ccagatggaa attcaggatg cgggccgtgc tgttcctgcc
    21181 gttctcacgc aggcccagcg tcaggtaatc agcccggaca ttcaggtcaa aactgtcctc
    21241 cacacactga taattcagat cagaatacgg gtccagctga cgtcttttcg ggtacagaga
    21301 gtccggatta tggttaatca tctccccgac aatctcgatg aaagcctgtg catccactgt
    21361 ctgtgtggtg atttttgcgc cgtgaaaaga ggcacggatg attttcacca gattttccat
    21421 ttccagaatg tcagcccggc tttttttctt cgagggagaa cagtacgaga taaacacccg
    21481 gtaatgacgc agggtcaggg gcagattcat cccctccggc agcgggaact gtgtcgctgc
    21541 cgctttcata taataggcac gggtaatggc gttaaaccgt tcagcctgtt cacctgacca
    21601 ggagaactca cgcagcccgt attcaatcct gtcaccaacc agctgactgg acatcagatg
    21661 gatacacaac ggaataccgc gcggcagttt ggtgcgcagc atgtgatcga gtgcctccac
    21721 aatggactta ttcgccccat tgatgggaat ggcttccagc ataaagccca tcgtggtgtc
    21781 attcatgaac agaccggatt cctggttgta atcacggtac ggcagcagac ggctgaactg
    21841 cgggaagctc atttcgccca gaacttcatt ggcctttgcg gattcgtcag gcagtttcag
    21901 tgctgtgacg agggagttaa ccgcctgagt gacggcctca agtgggttat tactcatatg
    21961 agtattcctt agtgcgttta aaaaaggaaa atatcagtct gtggccgtgg atatatcacg
    22021 cagtgccatt cctgaaacca cagcacaaat aagagatgga ggcttttaac aggaaaccag
    22081 gattaacgtt cctttactga atgagcatca tttaatgcct gaatttgttt ttcgcgaatc
    22141 ttattgcgct cattttctct tacccgtttc acctctgcca tttcgtttct ttttctgcat
    22201 tctgatgcga ttttatttgc cttaccaagt gttaaaggtt cagactgata attaagtgtc
    22261 tcctgaaatg cttctgctgc acagggagtt tcctgtaaaa tctctgtcag ttgttctggt
    22321 gttgcaggtt gttgcgcaag gtaaacgatg taaaaaggaa tacatacaat catagccata
    22381 acgccaacaa atataaaagc cagaacagat tttaaatata tcgacttcat ttcattttcc
    22441 ttcgaagcag taagcactca ttgcccacca ccatttagtg tttccatcac gctgttgaaa
    22501 ggttcatttg ttttaacttc aatacactcc cctgtatttc caaagcccct gccgccatgt
    22561 gcttcgattg tccgaagaac ctgcacgcat acgtccgtat ttggcatggg tgtaatattc
    22621 cagcttccgc caccgttaat attttttatc agcacaagaa aaataaaggt atgcattctt
    22681 tattatctcc gcttgtatat taatcactga atatcccttc agttaactta tgaggtcata
    22741 aaacactgca gattttcatt cagatttact ctcttcagca aactttactc tcttcagcaa
    22801 acttccggat ggtatcctgt tcacgctgac gggtggcaga ctcctgcatt gcatccactt
    22861 tctcctgcca gagacgccgg atatcctttt catcccgacc agtcagtttt ttaccggaca
    22921 gcagacggtt aagcagttcc tgtctcgcat ccggtacgtc ttcagtcaga cgcataagct
    22981 gaagcaggct ctgacggctg agaaccatga gtgattccgg caggagggtg aagaagaaaa
    23041 taaaaaagca taccgcacca cagcaggcaa ggaccgcaaa caggattttc agatatatac
    23101 catcaacgcc gaacgcagca ataatacagg gaatccatgc aatacacgca caatacgcca
    23161 ggatgttatc tattcggtta tgtctgcgat atatctgttg atgttcatcc agaatagaat
    23221 ctattttttc agcttctgat ttatgcatag tgtttcctct gcgtttcctg ccagagacgc
    23281 cggatatcct gttcatcccg accagtcagt tttttaccgg acaacaaacg gttaagcagt
    23341 tcctgtctca catctggtac gtcttcagtc agacgcataa gccggagtaa ttcctgacga
    23401 ctgagtacca tgcgtgattc ctccggtacg ctgtaataat taaaaatacg actgaatacc
    23461 ccacaacagc aggcaacgga caggcacagg gatttcagat acaggccaga aacacagaat
    23521 gtggcaacac cacaaagaat agctataact aaaaatccct tacccagtgc catacacaaa
    23581 gaaccctgtt ttctggatat ccgctcatgc tcatccagaa taaaatctat tttttcattt
    23641 tctgatttat gcatagtgtt tcctctgcct ttcctgatac gcctgacatt caacgcacaa
    23701 cgtcacaccg ggaaatattt tccgccgggc ttccggaata ggatttccgc atgcttcaca
    23761 gagataaaca ggaataccat gaacatttat ttcctggcgt atccggttta ttcctgtcat
    23821 ggtcagttgt tctgtcactg aatatgcttc atcggcttca tcactcacgg aatattcctc
    23881 ccgacatgtt ctgacacgct accgcccatc ccttttcacg cacagggcac tcgttttaca
    23941 ggcggatttt gaatacagaa agcccgccgg cattgccggc tgatatacaa cttcagggcc
    24001 agttaattaa tacgtggttt tccccacgca gacggtttaa taacaaaaaa cacgctggat
    24061 ggctgatgat aaacatcctg gttatcaata taaggcgcta tccataatgc tgccgtctgc
    24121 tcacccgttc ttagcggacg aggaggtgtt accggcgtaa ctgaactgac cggaacaacc
    24181 gttttcactt cccgggcagc ggtaaacagc gggcgtggtg ccagcagttt ctgctccgga
    24241 tgtgctgtca ccgcaggtct gctgccggac ggagtggtcg cggtgacggt ctgcaccggc
    24301 attgtccgga agttcccttc agccaggcgc ggcagtgatg ccgcaaccgg ctttgcttct
    24361 gaggactgct ccagtttttt ggccttctca ttggcctgct ccatcgtcat acaggtatcg
    24421 gatgtggtgg cgttacactc aaattccgta tttgttcctg cgcagccgga aagtaataag
    24481 gttcccagca gaggaataaa taaagaaatc tgtttcattg cccttctcct tttttattac
    24541 tccccacaac agcagaaatt aactctgctg tcagatttgt tttccctgca cagggatgtc
    24601 cggagaccac tgtatttccc ggagaagaaa ataagctcat cgacattccc ttcttcatca
    24661 caccgacagc agccatatta aatttgtgcg gcaatgattc agaactgaac accttcggag
    24721 caggaagaac tatttttgtg gtgcagttac tgagaacagc atgcataaaa tcatctgata
    24781 cgtttttatc cggcacaacg cgggctgtaa taacattaat attacgcatg ttcattttta
    24841 tctccgttgc ttctgtttgt tcggtcatca gggaaataaa catggcattt cctgattttt
    24901 cccggcaggg aagcgtcttc cgtataaacg tccttcagat attcatctgc ggttaccacc
    24961 cggatacatt tcatttttcc gggcagcacc ggatatttcc cggtgatcgc tgccagcgag
    25021 acatacatca gtgccagtgt tgcccataat gcggtgacca gttgcatcag tgaccggtca
    25081 gtctccctga cataatctgc agcatcagcc gcgcggtaat ggcagatatc ctgtatttct
    25141 gatctgcctg aagaatcaga ggaagaaaac cagcattcac tgaccgggaa ctgtccggca
    25201 ggtgctgttt gtcttacgaa gttaatctcc ttaaccagta atttccccgg cgtggtgttg
    25261 tccttccata aaacaaacag agccatcagg gccattgtgg ccagtggcag aatgacggtg
    25321 tacttcagac accagaacag tcctcgcaga acccgggcga caacataccg ggcggcatgg
    25381 cacgcctgac gggacgacat attatttgcc atcgttgcct cctccctggc tgccggacat
    25441 aaactgctgc ggattcaggt tgcccagctg cttcagcatc tggtcagtat taaagccgtt
    25501 aagatgactt tcagctgatg gcggaacagg aaccggactt tccggattat cttcttccgc
    25561 tctgttctgc gtgcgctcca gcgccatctc ttccacggtt ttcagctgga agccgtcctg
    25621 gaacaccacg gtcacttcgt tgcccgcacc aatcggtatc accgggtgat actgttcggc
    25681 acgtttgatg tagtagtcac tgagcgtctg tgcggctttc gatgtgccgc caccgatacc
    25741 cattttcagg acatcaccag ccccgtaagc ggctgtggca cccagcccga cagccggctg
    25801 ggaggcgcgc tccattccct gaccgatacc gtcaacaaat cccgcgcccc atgcccagcc
    25861 gaggattttg ccgttacgca tcaccacttc gcccttgata ccgtttttac cccggaagct
    25921 gacatgcccc ttaatcggca tatcaatagt tttgccgtcc ttcaggcaac tgatattgcg
    25981 ggtgcgtaca atggcacgct cactggacac atccccccag gcttccagcc ccacaaaaca
    26041 tcccgttgcg tcatacgtct tgctgttcgg catttccacc aggccggtga tacgcaactg
    26101 catcggcacc gtggattcat taccggtgac tgaggcattg gcatccgccc cttcaatcag
    26161 catggctttc gcaaaacttc ctgacggaat gtacggcagc gatggtccct gttttccctc
    26221 attacgggta aacaccttac gctgtatccg gttaggcacc ggcacagact ggtacgtcac
    26281 ctgtggtggt ggcgtgacac cattcccggg ataaaacgcc gtcggcggtg gaacagcaac
    26341 gctgccctgc ggcgggaagg atacgggggt gtttcctggc tgtggttcgc cttccggccc
    26401 gggcggtgag gcaggcatct gaggtacagg ctcaccggtc gccgtgacgg gattagcacc
    26461 cagggctttt acctgctctg ccagggcggc attgtcctgt cccagctttt caatacgacg
    26521 ctggtcatca ccgcgctgtt tgttcaggac atccagttca cgacggattt cctcatactg
    26581 cttctgcatc tgcgctgccg tcacctgcat ctctgtggtg gcatgctgac gcaccttgtc
    26641 atcaaaggtc gtatccacca cacctgtcat atccggcaca ggctcctgtt cagccaccgc
    26701 ctcaccgtta ccggacatgt ccacatcaga cagatacagt accccaccaa tcgcggaggc
    26761 tgcaccgaca accacaatcc ccagccacag gtactgcttg cgtttcacaa tggtattgat
    26821 actggccatc agttgccctc cccgtcacgg atgacataca catccatgcg cgcacctgcc
    26881 agtaactggc tggcaggctg gctgaacatc acggcacggg ttcccggctg ccagaagtca
    26941 ctttcccgga tattcagggc agacagcgtt ttattttcca cggcaaagcg gaccatcttc
    27001 aggtgattcc ccgtccatac ggcatctgcc actgaagaca gccccgccgg ggcctgcagg
    27061 gtttcctttg tcacaggaac ctgataccag cctgcaggta attttccgcc acggacagcc
    27121 tggctgatgg tcacaagcag ggattcgtaa ggcgtggacg tttcccacgc tccggcttct
    27181 tctccggttc cgcgcaggtc actgaccagc tgaatggtac gccccgcgcc ttcacgggga
    27241 acagcctgaa tggaaaggtt cagaccacgc tctgtttcca gaatgaacgt aaagggcttt
    27301 ttgttgacgg tggccaccac cacaccgccg gaggcggtct gctcattatt ggtcagggca
    27361 ccatccagac tgttcacggc gataatccgg tcaccgggaa cggtaaacag attcgggctg
    27421 gtattgctga tggcaatact ggcctgacca ccgttcacca ttggcaccac agtgggggcc
    27481 agcgtaccgt ttgcggccat cacgctgcag gaaaaaaaca ggccgccgaa tattattgcc
    27541 gtattatttt ttctcatcgt ctgtttcccc gaaattatcc agccaggtca ccccgttttc
    27601 ccgcttcaga ataaggatgt aatgtttgat atcagtaaaa ggttcggagt taccaatcca
    27661 tgttttccga attccacgga tttccacacg accatactga ggccagacac ggacactggt
    27721 ctggaaaaaa gcagaattca cgttatcttt tttaatgagt ttcgcttctt cagccagaat
    27781 aactttcatc tggttctgtg cacccgggcg gatatattgc agaagcgcct gatgtgaagc
    27841 atcgacgatt tccggtgaga cattcagacg gagggcaata aatgacagcg ccatctgctg
    27901 caaataagag gcatcggcac tgttctgtga cacggcaaag ggcacattaa atcccattgg
    27961 ggtcacggca gtgcgttgct cattctgaag acggtagtta ttcaccccct gaatgaggtt
    28021 aacagagagg ctgagaacaa taagcactga cataaatata aaggcgatgg ccattacacg
    28081 actggtactt aaacgggcac cgtgttccat ataacaatcc tggtatcagt tctatttaat
    28141 ccactgccgg aaacacgaat cgggaacatt atgaaaaata ccgcgcagca gggctgttgg
    28201 catataccag taaatcaggt cacgtaacca ggaactgccc cgcccttttt tcagtttttt
    28261 aatcccgaaa taaaccagaa ccgctgcacc aataccgaac agatatttcg atgttgtgat
    28321 accccagcca acacagattg ctgcggggat cagttcatcc agtggcaggc caaaccagcg
    28381 gctctggttg gtcagtgttt ccgggaaacg atatttctta agtttattct cgtctcccga
    28441 catcgtttta tttcctgtca gaggccaacg acgctcatac ccacagcaat aaatacagag
    28501 atgatggcaa agccgaccag gaacttgacg tttttggtca tcatgtacat gaccgcaccg
    28561 accagcacct cagccagaac aacccattta acgatactgg agtccttacc gaaggtcgcc
    28621 ttaaccgtat cgttaccttt agccatcagg tcgcctttac cagcagccat agctaactca
    28681 gggaacaaca tcatcagaac agcaaccggg atcactgcac gaaccaggcg aagcatattc
    28741 agacgagtga atttagaaaa aaacgattta ttttcaacgg gcgcagaagc accctgaaca
    28801 cttaaaacag tattcatatg attcctctga ttaatattga cagatgaaaa ctaaagttct
    28861 ttaaatgttg actcgttctc ttcgatcacc tccctgaata tttcttcatt ataaaaatct
    28921 ggataacgtt gcaaatgatc ctgcaacctc accaatgctt cgtttgtttt cgttcttcca
    28981 ctacgctctc tggctccaag taataattta ttcgtttcct catcaagggt aagtaaaact
    29041 tttctacttg gcgcagatct taaagctcct ttaatcaccc tccctcccat aaataaaaga
    29101 gatttagtca aaatattcac ataagacgca cacaaaaata aacaacaaaa tagtaaatta
    29161 cgttaacaaa aaccggcact tttagtgccg ggaatgctta catttcttta cagtgaaggc
    29221 tgttaatatt actagcgaag tattgatata gataaaataa cacatcagag attatagcat
    29281 atcgcaacca ctcatccctg tctgagaaac catagtgttt tttaaaattc ataaatattt
    29341 ttttagattg gtcaacagag atatccattc tctgagaaga atactcatga ctataaccca
    29401 aaagatacgg tattatctta ttaaagcgat atttgtcagg ttcacaacca aactccttgc
    29461 cctttataaa aaagccacta actacgtatc tttcaaagaa tctccagaca aacaaattca
    29521 agttattcac ttccgtactt tcaaccacca catcccatga tacatcattc aatattacac
    29581 cattaataat actcactcca ttcgaggacg agaaagcatc aacttcagca gtatgaaata
    29641 aaatttgcgt atcaatatca ataccagaaa accatatttc agaatcacta ttctgacact
    29701 ttattaagtg taaaaaaaca gagtttgagt ctatgaattt tcctgactct gtgcggacac
    29761 agactggata ttttgcggat aaaataattt ccggcaggcc tgattgacgt atgagtgctc
    29821 tttctctagt atccatcgca cacataggaa cctcctcaaa ggattctatg gacagtcgat
    29881 gcagggaggg acaagctccc tgcatcggcg attttcaagt tcaaatttta acgtggcatt
    29941 aaccacgtaa ttaatatact aattattctg attattgtca tcagtttgaa ccattaaaaa
    30001 gtctgatatt tgaacaaagt caaaatttat ttccctttat tcatcatcat tttttggaaa
    30061 gaacctttcc atctcagccg acaccttctc tctgatatct tcaaccatat tggcatattc
    30121 aaactttgga tttcctgaga catgaggact gagagactca atacccaaaa ttttcgctac
    30181 tgatgactgt gtttttacaa cgcattcaag aagtagttta ttaaactcgg cctgattaaa
    30241 tgcagactct ttgcgctcca tctgagcctc atatacacga agacccagtt caagaagcat
    30301 tgaagccgtt gctgaaaaac tgacatcttt ttctctcgcc ccctcctgtc gacgtttttc
    30361 aataatcgcg tttatttttt cataagcatc attgctgata tacagattca ccttagccat
    30421 tagatattac cttttttaaa cataaaacaa agttaatttc gaaacactag aattcatagt
    30481 gcaaacaggg acgcaccgct agcagcgccc ctgacggtat cctataaaaa aacacaccgc
    30541 gccgctagca gcacccctaa tataaaataa tgttttttat aaaaatagtc agtaccaccc
    30601 ctacaaagcg gtgtcggcgc gttgctgtag ctgcgttaac gacgctgctt taaaaaaatc
    30661 agatttaaac aatataaatc cacaaataca actcaatgat attaaagata aatcagcaaa
    30721 aacttgtttt tgcgtagtgt gtggtgcttt tggtggtgag aaccaccaac ctgttgagcc
    30781 tttttgtgga gtgggttaaa ttatttacgg ataaagtcac cagaggtgga aaaatgaaaa
    30841 aatggatgtt agcaatctgc ctgatgttta taaatgggat ctgcgaagcc gccgattgct
    30901 ttgatcttgc aggtcgggat tacaaaatag acccggattt actgagagcg atatcatgga
    30961 aagaatcccg ttaccgggtt aatgccatcg gtattaatcc ggtaacggga tatggcagcg
    31021 gactgatgca ggtagattcc cagcatttta acgaactggc ccgctatgga attaagccgg
    31081 aacatctgac aacagatccc tgtatgaaca tttataccgg tgcttattat ctggcaatag
    31141 cctttaaaaa atggggcgtc tcctgggagg ccgttggtgc atacaatgcc ggattcagga
    31201 agaccgaacg ccagaaccag agacgtcttg cctacgcatc agaggtttac cggatttata
    31261 cctggataaa gagcagtaaa ggcatccggg ttccgaccac gaagaaatca ctttcccaaa
    31321 ttaacagtgt gcagaaaaat taatattgta tacattgtca aataccggtt atgccgcgac
    31381 agcggcatgg aaggccacac acaaaaatat catatgagtg ataatgctga ctggccgaag
    31441 ccccgcaaag cgggatccca tcttatatat atccggtaac tgcgcagcct cctgccagac
    31501 catttttcgc gggcacaata tttacagact accgaaaagc cagttgatga aattgaggca
    31561 tatcagacga cacagcaatg ccgcaggatg ataatataag taaagccccg aaaaattttt
    31621 cggggcttta cttatattat catgacttcc actccctgaa ctgttcggca atcatccata
    31681 atgctttgtt gattcggata tcaccgtcaa tgcctgtaat ggctcttgtt ctggtatttt
    31741 taccggaggc acttcgcccc gataatccac cttttatcat attctcctga acccgctgcc
    31801 aggttgtcca gagatcgttc tgtttatcct cccaacggcg gggagtaatt atttgttctg
    31861 gcgtcactgg cgttttattt tcatcttcgt acctggccat caatgcagct ctgccaaata
    31921 aatgttgttc gtcactgtta agatgtatct ctttcattgt ctccatattt tcagtgactt
    31981 tatcaaagac accgagcact tcatacgctc cctcaataac ctgcccgaca atatcacctt
    32041 tatgtggaac gcggatttcg ccaaaattat tcccacacac caggccattt gtgcagacaa
    32101 aacgaaaaat tcctgggatc atctgataac tggatgaacc atcatgtgaa ttaagcagga
    32161 taatttcagg aacttctttc ccgttaatat gcccttccct gcgaagacgt aacatatgtt
    32221 tactgtattc gcggcgtccc aaatcacgaa cccgactctg acaggcaaag aatggctgga
    32281 aaccttcatc acgtaactta ttgatgatat tgatggttgg gatatatgta taacgctcac
    32341 tccgggattc atgtttatca ccagaaaaga cagatggaac aaactgcatt aattcgtcat
    32401 ccgttaacgg gcgctcacgg cgaatagaat tatatcgacc aaaacgggaa gctaatcgca
    32461 taacagtaac tcctttatga aggcataaaa ttaataaaag ttttttcgct taatatcatc
    32521 ccccctcggt tatcgttaac aaaaagacaa caattccgag gggaaggacc atcaaatcag
    32581 gtcagttcgg gactgaccaa cctgccgagt gaaaagcagt aacagcgcga tcaactcatc
    32641 agcgtttttt ctgacaagag gagtatgatt ctccaggcta tccagtaagt catgcagctc
    32701 attattgacc tggtggagca ccttaaagag tgcaccagaa tcccggcagc tctgatatgc
    32761 aagatttgcc tgaacagcac gattctgcat gatcccgatt tgtgcgattg cgtctttctg
    32821 ctgatcgcct gttggctgat aagtggtgtt aaaagaaaat gcgctcataa ttcctcctgt
    32881 atctgatgtt ttcagccccc atcatctgtt gagtttcggg agccgcggtt ttgacgtttt
    32941 gcggggacct gccttttgtt ccctccggta ttcaccatca cctgtttgag aaccttcgcc
    33001 gcaaggggca tcactgccac ggcctgacgc acctgtcctg ggcagaacgc aaaatttttt
    33061 accgggtttt gggtaaaaaa ttttgtgggc tgttcattta ccctggacag tcagtgcggc
    33121 aggttgtggc tgtctatgcc cccggcgaca ggggcttaaa caggtgaatg gcaccggaca
    33181 gaacaacggg cagggacccg ccgcaacgac gtgaagcgcg gttcgcgaaa cggcgttgct
    33241 ccttacatcc cggcgtcttt tgccgggatg ccgggagcgc aggccacggc gcgacaaggg
    33301 tggcgcgacc gttccggtcg ctgcaaaccc gggtttttgc gtcagggatg gaagcccgcc
    33361 agggcggaga cagcttcgct ggctccgttc acgacagccc gccccgccag ggggacgccc
    33421 ggaaaaggca taagcaggag aacaacaaaa cagacaataa tcagggcagc agtaatcaga
    33481 gtcagagcag taatcagagt cagagcagga ggaaaaagag aataatcaaa gggaaggtga
    33541 tgtgatttct ggtaagtcat aaacagagaa ggcgggggaa aagaacgggc gccgcaggcg
    33601 cacgttcatc cccgttcccg gagggaaacg gccttcctgg cagtctggtt gttcatgccc
    33661 gtacaatgcc agcaaggaga aaggggccac cggcgaacca gcagcccctt tataaaggcg
    33721 cttcagtgca gtcagaccag catcagccct gaaaaggcgg gcctgcgccc gcctccaggt
    33781 tgctacttac cggattcgta agccatgaaa gccgccacct ccctgtgtcc gtctctgtaa
    33841 cgaatctcgc acagcgattt tcgtgtcaga taagtgaata tcaacagtgt gagacacacg
    33901 atcaacacac accagacaag ggaacttcgt ggtagtttca tggccttctt ctccttgcgc
    33961 aaagcgcggt aagaggctat cctgatgtgg actagacata gggatgcctc gtggtggtta
    34021 atgaaaatta acttactacg gggctatctt ctttctgcca cacaacacgg taacaaacca
    34081 ccttcacgtc atgaggcaga aagcctcaag cgccgggcac atcatagccc atatccctgc
    34141 acgctgacca cactcacttt tcctgaaaat aacccgctca ttcaggctgt ttacgggtaa
    34201 tctgtgtgat tgttgccgca tcacgccgcc tcccggagtt tgttcggcac catccaccgc
    34261 tccatctcac ggacagcgtg accggcctct cttgctgcaa gactggcttc cgcaataacc
    34321 tgccagaacg gcatatcctg ccactgccag cgttccattc cggccagtga cggaaaacgc
    34381 tcaccccact gccagaacag ggtttccagg tcatgctctg ccacccggtc actccagcgt
    34441 gcataccagc ttccgactgt ttccggcgaa tggaatgcca gttcgatttc agcctgagcg
    34501 accagcgcct ggtacgcacg ggctttctgc tctgcctccc tcgcctgctg gcggctgtac
    34561 ttctgcatga ccagcgcgga tttatggcga ctgcgttccc tgacacgaaa ccgcaccgac
    34621 gggaacaagc gttctgctgc atcgcctgga agaggaagga ggcagacctc gccctgtgtt
    34681 tcaatcagca catcgatggc atacagccac tgaagacggc tgccgtgcag ttcttctgcg
    34741 gtcagggacg gcatgaaaaa tcgcaggtca gacagcgata tcctgcggct tccgttcaga
    34801 cagcggaaaa acgcccccgc atacggatat tccgccagcg tgtttccctg gtggcggcgt
    34861 ttttctgtct ccgccaccgc ccgccatgcg gcctgttgct ctgcgctgag gggaaccagt
    34921 gcctgtgaac gtgcgctcat gcctgacctc cttccgccat caggatatgg atgatgtcac
    34981 tgacgctgta cccctgcgcg tccagtgacg ccgccagtgc cagtgaatgg ctgacggctt
    35041 ccggtgcaaa ccgccgctgt acctgaacca ccgcaaacgg ctcaccaccg gcattcacca
    35101 gaaccagcac ccagacctga gagacatcgc ccggtgaaca taacgccagg tgaaaacgtt
    35161 cgtgggcagg cgtgaaacgg acctggacgg ggaaaaatcc gccaaactcg ctgccgtact
    35221 cgccgttcat cgtccagttg tccggcgcat ccagttcccg catcacggcg tgtgtcagct
    35281 cacgacgttc atcacttccg gcagcccgga tatcttcata ttcctgtgcg ctcatggtct
    35341 gtaatacatt cagggtcagt tcagttttca tcacggtatc tcccttgctg ccgccccgtc
    35401 tccggcgggg cggtgtggtt gttcatcagg cggcatcagc caggttatcg gtgcgttcag
    35461 tggtatctgt ctgtggatgt ggtgcacaca tccagccagg cacccagcgg ttgtctttca
    35521 tatggtgctc tgcatgttca gccgcatcac ctttcttcat cttctccgcg tcccgtgcgg
    35581 caccggacag ccctgcctca ttcagggctg cgataatctg cggctttttc aggtgtccga
    35641 agaagtttgc ttttgtcggc tgccaccagt cgcgcatgtg aaagccgatg gcgctttcca
    35701 gcgtgtcaag cggactgcgt gacgtgtgac cacactcacg ggtctggaca ccgttaaggc
    35761 tgcatgcggt gcagaaactg agcagggata acagcacctc ctgactgagt gacaggaatg
    35821 tcgtcatgtc ccgtgaccat ccctccggta acagggcagc aagacgggct ttttctgcca
    35881 tcagcgccat gaatgcggca ccttccttcc ccgatggcgc atcgctggtc agcgaataat
    35941 gtttacattc caggctgatt tgtgctggtt tactgtacgc tccgctgcca aacacattca
    36001 ggcagagcgt ccatgccagc agtgtcaggg atttgtccgg ttgctgcatc agtgctgcct
    36061 ggactgccag cgtgcgttcg gaagacattt tcgtcagcag tggcagactg atttcctcca
    36121 ctgacgcttt ctcctgcacc tgttccgtac ggtcagcgtc gtcagccacg tcatcctcac
    36181 tgcgcaactg cacaccacgc tggacacaca cgttgccata acgccaggac accaccactc
    36241 cacctccggc acgcatctcc ggcgtccacg ccctgacctt cgccatgcag cgcatcagct
    36301 tcatttctgc ttccagcagg tcagattcct cacactggtt ttccagcgcg tcgtaacgcg
    36361 tcatcagttc gttcagacgt tcttcttccg cctccgtcag caccgcttcc ggctccggca
    36421 gacagcggta tgttccggca tcctcacggc actcaccgac aggctccatg cgtccggcgc
    36481 accattccca gccttcggct tcccgaagga actctgcgac agcctggagt ttttccagca
    36541 gggcggcatc gagcgccacg cagtccacat agccaccttc gtcgtcgctg aacaaatcgg
    36601 tgcgcagttc gtctggcgag aaggcatcag cccccacgaa gcggaattta ctgttccccg
    36661 ccaccgccac ttcactttcg gtcaccagac gacgaatggt ctgtacttcc ggtttaccgc
    36721 cccatcccga ctgacaggcg gcttcaaaca cctgcacctg acgcgcggtg tcgttctcca
    36781 gcgccagcgc ctgacagtgt tcggtggtga tgcggtcttc tgccagcgca tcgaggatga
    36841 caggcgcaag gtcagccagt ttcagcattc gctgaacgtg gcggggtgaa tagcccagca
    36901 aatcaccgat ttgtgcaggt gttttgcctt cctgcgccat tgcgcggaat ccggcaatct
    36961 gttcggcagg gtgcatatcc cgacgctgac cgttttcagt cattgatgca gccgtcgcca
    37021 gctcctgcgg aataaccttc acgcgtacag gccagtcagc cggaaggatg ccacgctctg
    37081 ccagcatgtt gagtgctgcc agtcggcgac cacctgcggc gacaccgtgg cggtcaccag
    37141 gcagggcatg aacaaccaga ttctgcaaca ggccaacgcc cttaatggac tccgccagtt
    37201 cgctgacgga ctctgcggaa tacggcaccg tgcgcacatt cagcggtgat ttaatcagcg
    37261 aggccagcga cacgctcact tcctccgtct gctccagcag ggcagacagg actgtttcct
    37321 gcgtttttgc aggtttacgg ctggatttac gctccgtttt tgtcttagac tctgtaactg
    37381 acatatattt ctcctttgac cgggatggtt gtatgtctgc cggacgcgtt cccgtccggc
    37441 acctctctta cttaagaatg ccgaaattcc ggatgacatg gttcatccag tacccggcac
    37501 cgtcctcaaa attccaggta cgccagacca ggccactgtt attgcggaca accaggcgaa
    37561 aatgcgttcc ctgatcatcc tcgataaaga cattccggta ctgtgcggca acggcttcag
    37621 cctgttcgcg ggtaaaggag ccgtcaggca gcccctgaaa tattctgaaa tattcgctca
    37681 tcctcatatc tccggtgatg ccccgcactg gacggggcgg ttgtcactgt ctttcgttag
    37741 aacggaatgt cgtcatcgaa gtcatacgcg ggttcagacg gctgctgagg ctccggctgc
    37801 gcggccttac gtccacggcc ttttgttttc gcgctccctt ttttcgtcgt gtcagcactc
    37861 tgtggctgac cattctgctg aggctgcggt tgcgcctgag cgttctgctg tggtgcactc
    37921 cccagcatct gcatggtgcc cgtggttttc acaaggattt cagtgacgta acgggtaata
    37981 cctttatctt cccagctacg ggttcggagt tgcccctcga tgtagacctg cgcgcccttg
    38041 cgcagatatt cacctgccac ttccgcgagc ttgccgaaca gcaccacgcg atgccattct
    38101 gtctgctccc gcatctcccc cgtctgtttg tcacgccagc tttctgacgt ggccacctgc
    38161 aggtttgcca ctgcgccccc gttggggatg taacggactt ccggatcttt tcccagacga
    38221 ccgacaagaa tgaccttgtt aatgccacga actgccatga tgtgtctcct tctgttgatg
    38281 ttttcagccc ccatcatctg ttgagtttcg ggagccgcgg ttttgacgtt ttgcggggac
    38341 ctgctctttg ttccctccgg tattcaccat cacctgtttg agaaccttcg ccgcatgggg
    38401 catcactgcc acggcctgac gcacctgtcc tgggcagaac gcaaaatttt ttaccgggtt
    38461 ttgggtaaaa aattttgtgg gctgttcatt taccctggac aggtgcggca ggttgtggct
    38521 gtctatgccc ccggcgacag gggcttaaac aggtgaatgg caccggacag aacaacgggc
    38581 agggacccgc cgcaacgacg tgaagcgcgg tttgcgaaac ggcgttgctc cttacatccc
    38641 ggcgtctttt gccgggatgc cgggagcgca ggccacggcg cgacaagggt ggcgcgaccg
    38701 ttccggtcgc tgcaaacccg ggtttttgcg tcagggatgg aagcccgcca gggcggagac
    38761 agctccgctg gctccgttca cgacagcccg ccccgccagg gggacgcccg gaaaaggcat
    38821 aagcaggaga acaacaaaac agacagtaat cagagcagca gaaaaaagaa gaacagtagc
    38881 agtagcagta gcagtagcag tagcaggaac aacaacatca tcagtaacag gcaaatgaca
    38941 ggtctgaaaa gacagaagcc cacagcagaa cgtccgcgta atgaaagggg cggcgcgtaa
    39001 gcgcggcccc tttcagcgcc accggcgcgc ggtcttttcc cggcgcgggc agacgggcat
    39061 ttatcctccc gcctgcccgg attcatcagg cacgcagacg aataatcatc gtcggaacac
    39121 gggtataagc aaatgtgccg cacggcagca cctcgcggac atcagaaaac ggtaacagct
    39181 tctcctgctg gcgcggcccg ttcagacaga cggcaaccag aacgcccccc ggacgcaaca
    39241 gggaaaaagc ccgcaggata tgccggatat cctgcccgtg actgaacggc ggattcataa
    39301 tgatccggct gtaatactgc accggctgcc attccatgaa gtcgccacac tgtaccctga
    39361 caccgttaaa gttttcccgc agataccgga ccagcccgct gttaatctcc accgcatcac
    39421 acatggcctc cggcgcggta tcccgaatcg cccgcagtat ggccccggtg ccggcagagg
    39481 gttccagaat gtgatcacgg ttgctgattt ccgccagcgt caccaggcga tcacagaccg
    39541 gggcaggagt cacaaaaagc tgagattcag cccggatttc agcaaccggc gcggcgttat
    39601 ccagccttgc gcgcatcagc cggatacgtt ctcttaactg actgtgcatg tcattatctc
    39661 cgtcatcccc cggggatttc ccggggggat ataccttatt tctgcgggat ttccgtgatt
    39721 ttcatgtcgg tgatatacac attcaccaga cggaaattat tgtccattgt gcggcggtag
    39781 cggtatgccc cgtggtcttc tgcttcttcc acactgcgca cggccttaca gtcccggggc
    39841 agggcggccc actgcgcctt tgtcatttcc cggaaacctt cccccggata gttgactacc
    39901 ggcggacgct tcgcggcctg actggcgacg gcagcctctt ctgccgatgg tgctttgtag
    39961 tccgtgatgc gatcgggcgt cactttcatc gtgccgctgt acccgagaaa actgtaattt
    40021 ggcgttgtga ctgaactcac cgccccgttg cttttgttca cgcggatgat agtcagccac
    40081 tcgccccggc tgaaaacctg tccgcccggc tcaaaatcct gtgtccgggt aaccacgccg
    40141 ccgctttcgt ccagcatggc acgctcatag atcaggcggt tctgatagtg gttaacccag
    40201 cgttgctgat gctgaatctg ccgttcatga cagcggatcg caatttcgcg ggcctgctcc
    40261 gtggtgatga tgtcatcatc cagggccgac cataacgacc ggctcccttc atactggctt
    40321 ttttctgccg ggcgcggata cgtatccagc gggaaacagg cgctgatatg gtcatgactg
    40381 ctgatgagtt ttgccatatt cagatccagc gattcagccc gccacatcgt cagatatttc
    40441 tgcgactgtg cgatggtctt ttcagccttg cgcaaatccg cttcgatttt tttgatacga
    40501 cgccagcgaa cgtccgggcg ttctttatac ttcgcgtgaa gcagtgccga ccgcgcccgc
    40561 tcctcccagt attccgcacg ttcaaagagc atcacggcac gtttcatgcc gttttcaata
    40621 cgctgcgcat cacgacgggc gcggcgttcg ctgtgatgcc ccacaagaat gggctgaccg
    40681 ggcgggatca tcgcggccag tctttccact tcatcaagtg cctgtgcgga ttcactggcc
    40741 cgctttccgc tgtatccggt aaaccgctcc gcccgtgctt cctgacgttc agcgagcgtg
    40801 ctgtcttcat cctcaatctc tccggcaagt gagaggagca cgtcttcccg gcccggtgtc
    40861 caggccggcg cgacaaacag cgcctgtttg ggtgcccagc gaaaaccggc atcatgcact
    40921 tttttatacg tctccgggtc aagacgtgac gcggcataca ggcgcagttt gttgtcatcc
    40981 ggtgagtagg tcgcgcggta catctgcaca gggctggcgg cttccggggc ggtcatggtg
    41041 attgctgcta atgtcataaa atgttctcct tccgtctgtt gccgcccctc gcggggcggc
    41101 tcacctcatc agttaaaagc gcgtttcagt tcgagtgtgc ggagcacctg cccgaaagcc
    41161 gtgtttttca tggcggggat gtgtacacca cgcccccggt gaaaactccg ggctatgtgt
    41221 cgcccgaagg ctttcaacac ggggtcaatt tccggcgtgg ggatgtcttc gcgtctgaaa
    41281 cggggatgac ggcggggctt tacagccgct gcggaaggtg aaacaatgct tacagtcata
    41341 aatggattcc ttatgacaaa ttggttaaca atgccgtcaa aattggtcgc acaattctta
    41401 cggcgtggat taagcaggtg cctgacctgc ttttttcata tctgcatctg acgctttccg
    41461 gtgttttcct ccttctgttc ctgcgttctc attacctgtc agccggatga ctgacagaac
    41521 aaataaatgc gtattgccgg agcctgaaat aaatcctgat ttttaaagaa catcacccgt
    41581 tgagtttcgg gagccgcggt tttgacgttt cgcgggaacc tgctgctgta ttcactgtgg
    41641 tatgaacttc acccgtttga gaaccttcgc cgcaaggggc atcactgcca cggcctgacg
    41701 cacctgtcct gggcagaacg caaaattttt taccgggttt tgggtaaaaa attttgtggg
    41761 ctgttcattt accctggaca gtcagtgcgg caggttgtgg ctgtatcagc ccccggcgac
    41821 agggactcat acgggtgtgt ggcacccggc aggcaatgag caggggcccg ccgcaacgac
    41881 ggagagcgcg tgcgcgaaac ggcgttgccc cttacatccc ggcggccttt tgccgggatg
    41941 ccgggagcgc aggccacggc gcgacagagg cggcgcgacc ggaacggtcg ctgcaagcca
    42001 ggggttttca cgcatcggac cgtagcggca gcccgaaacc gcgccagcgg gttcggcgga
    42061 gactggcagg tgcgccagca gctgcctgtc ggagtcgggc ctgacgaccg gatgccgcca
    42121 ggcagccggg agccgaaggt gcgcccggca gacagaacaa cactgcagta gtcagaatca
    42181 gagcagtaga taaaggaaca acaacatcag gaacagttcc ataacataag aaagaaacac
    42241 tcctgagtca gacacatcac agacgggaag ggtaacgggc gacgcgccag cgtggcccgc
    42301 ttcccttccg gcaggcgtca gcctgccagg ggttttcgcg cgggctgaca ggcggcctcc
    42361 accgcctgcc cctgtatcac ctgtttatgc tgccctgaag gatgtagtga ccgccgagga
    42421 atacagcctg ctgattcgcc acaaaatcgc agatttcatc aacaatctgc gcagtggtga
    42481 gcggtttctg ccccatttca cgacgccgcg cattaatcac ggctttgtaa ttgcgcagaa
    42541 tttcgcgggt ttctgtgctg atattcatga catgtgctcc ccgtccagcg cctgtaacac
    42601 aacgtccatt gcttcgcgtt cttccctgac gaatgcgtca ccgtcgcccc cgttctcgct
    42661 cccgacggca tacaggcgct tcatttcact gcctgccagc gccatcactt tttccagcat
    42721 caccgccgga actgtcacct gacggctggc gcgcttttct tccatcagct cacgaacaac
    42781 gtcgtgaaca atgctgacat ccgcaccatg cgcaaaggca tcgtccagtc gctgcatgac
    42841 ggtttccagc tcatcgtctg tcagactcca gtcctgggct atgtgacgca catccccacg
    42901 ggtcagcacc acggcaaaaa cgcattcact gtccggcacg tctgtgttca gcacttcgcg
    42961 gataatttct ttaacagtac agtacatgtt ctcgtctcct ttacaggggc ggcattgccg
    43021 ccccgtccgt taatcagtcc agtgccgcgt aaatcgcgtt gcattcgggg tgaaattcga
    43081 tgtgacgcca caactgcgca tcgcgcatcc ggtaaagctg ggtcagtcct gcatccccgc
    43141 tgtcgtggta cagccacaac tggcggttaa tcacaaggga agtcagaatg atgcctgcgg
    43201 catcagcact cacggtacgg tcaaaccagt ttgcaccgtt caccatgtgg aaacggtcgc
    43261 catccggcat catgtaaccg ccaccgtccg gcaggcggat gaagtgccag aagcctccct
    43321 gatattccgg catcattttt tgcgccagcg cgtacacata cgcctcggca tacagaaaat
    43381 cgttgccaaa cagatacggt agaaaggaga gacggtcgca ctcttcaacg ttcagagtga
    43441 caggtttcgc atattgcatg atgttttcct ccgttgttca catattccgc gacaccgggc
    43501 ggtgccgtgc ctccataaaa gccgggcgat aaggcgggga tgcacagggc gcaagggaag
    43561 ccgcagcctg acgcacacgg ctggcgggag ttggagggtg agaccgcgaa atctgagcgg
    43621 gtgaacgacc ggaaaatcac ggcagacggg aaggagggac cgggtgaccg cagcggcgaa
    43681 gcgtaccctt gcatccctgc cagcccggct aagatggagg cacggcacag cccgcacacg
    43741 acgctgaggc cacataactg aaaaaccaca aggccatgcc ctgcatggcc gggtaaagcg
    43801 gagccggcgg cgacttcctt tgtgctctgt ccggggaccg gcaggaaaaa ggcggggatt
    43861 taccccgcca acgtcatcat ttccggattt ttgccagggc atccgccatg ctgcggattt
    43921 cggcccgggc ttcccggacc acctcccgca gaacaccacg gtcaaaccag cggcggggcg
    43981 gttcatccgg tatacgcagc acatcgcagg ccagcccact ggacaggata tgctcaatac
    44041 aatcgctgac caggtacatt tccaccgacg aaaaacgtgc tccccggttc acgatggcat
    44101 cgagcaccac gggcaggttt tcgtcatctt catcgagcca gcaggggtga tcgctttcct
    44161 tcacaaccag acgaaagcgg cgcgtctggc gagtcagcaa cgtctgaccg gtggtggtca
    44221 gcgccgcccg gatacgccgc gcatcttctg ccacccggca actgacactg cgtaccgcat
    44281 cgcgcaccgc cgcaagtaca gccctgattt cgtcttcggt cagccattct ccggcatacg
    44341 ggaaagacac ctgcgttccg tctgccggaa gtaccggcag cgactgccac tgactgccgg
    44401 acagtaccag ttccgccccg gttccggtca gtttctgaat ggcggaggcc aggagcggcg
    44461 gcagtacgcc gccgtgacgg ttgataagta acgtcgccag ccggggcgca tccagcagaa
    44521 cataaatgct gatccagccc ggatacttcg ggttacgctg gtcaacatcc gtctcttccg
    44581 gccagccctg cgccagcagc aggttgcggg cgtgacgaca aatcagtgcg ttcagcgtgc
    44641 tgttttcgtt catgattaag cgttctccct gtatcgccgc ccgcagggca ggcggcgata
    44701 acatcagtcg ttttcgtgtg tgttgttttt cagcagggtg cgggcctgat taaccacctc
    44761 ctgacggcgg gtgacgcatt ccgggacggc gaggccatga tcccgggcgg cccattcacg
    44821 tttccacaat gcctgttcag cggtctgaat cagggacgcc agcagttcag cagggacggt
    44881 caccaggcgg ttttcgctct cgttgcggat aatctccatc accgcagcgg aagaaatacc
    44941 ggactcaatc cgctggtctt ccgggatgtc ctccaggcgt gccaggacgg tcctgatttc
    45001 atgaccggtc agagaaattt ccataccgtc agccagtgcc tgaatctctt ccggggacca
    45061 gataaccagc atcagcggca tgtctcccgg atactttact gccagctccc ggcatagcgt
    45121 ttcgcatgtt ccgtacattc tcgtctcctt ctcaatcttt aaagcacgct tccagttcca
    45181 gaatttcggt atatgcctcc atcaggaggc ggtttgcgcg aaacagcgtc tgacgcacca
    45241 gacgttccgg cagggtgtcg gcattaaagc tgtaccgctg ctccatctca ttcatggttg
    45301 tacgcaggtc gcacatggca ttggccacct ccgccacttc ggcgcgcagt ttttcgtgtc
    45361 ctgcatagtt catttacgca gcctccggca taaaccagtc atcattcgcg gccccctgct
    45421 gcatggcccg ttgcacggcg gcaaggcgtt gctgcccggc acggtgatac tgctcaagca
    45481 gctcgatacc gatataccgg cgtccggact ggagggcggc gacgcaggtt gagccgctgc
    45541 ctgcaaacgg gtccagcaca attgcgttcg ggtgtgtgaa gctctcaatc agcggttgca
    45601 ggctggtaac gggcttttcc gtcgggtgat ggcgattgcc cgaatatttc cagcccagca
    45661 cgtccggcag cgggttttgt ggcagacgtg gacggccttt tgccaggatg taggcacatt
    45721 cgtggcgata gcccacatat gcggccttcg atgtgtagtt tttggtgaag accaggtgac
    45781 caacaacgct gaatcccgca tttttccagg cggccataaa gcgatcgacg cggttccagc
    45841 catagaagct caccattaac gcgtcttttt tgagtacgcg gtacatttca ttacaggctg
    45901 gttgcagcca ttcatcggtt ttatcgccgg cgatggtgcg cccggaacgg tcacggaaac
    45961 cgacgagata cggcgggtcg gtgaggatga aatcgacagc attgcccgga aacgtggcca
    46021 taacacgcac gcagtcaccc aggataaaac gggacataac agcactcctt ctcttacaga
    46081 gggcaccggg agggcacccc tgtggtgccc gttccccgct gcctgtgggc tgttcaccgc
    46141 gtccggtaac gggtcaggcc gaccgtggaa taccgcagcc gtcgtctttt tgacggcgag
    46201 gatatgccgc gaaagcggct tgaagggcgt tcatcagaaa agggcgacgg gctggcgtac
    46261 actgtcacca acgccagccg ggagcggtaa aagcgcccgt cccgtgcccg gccggacgcg
    46321 ggacactgac cacaggcagc gttcctgccg cgccgcgtca gccggcgcag taaaaagagg
    46381 agcccggcga catcttttgt tacccgccaa acaaaaccca aaaacaaccc atacccaacc
    46441 caataaaaca ccaaaacaag acaaataatc attgattgat ggttgaaatg gggtaaactt
    46501 gacaaacaaa cccacttaaa acccaaaaca tacccaaaca cacaccaaaa aaacaccata
    46561 aggagtttta taaatgttgg tattcattga tgacggttca acaaacatca aactacagtg
    46621 gcaggaaagc gacggaacaa ttaaacagca cattagcccg aacagcttca aacgcgagtg
    46681 ggcagtctct tttggtgata aaaaggtctt taactacaca ctgaacggcg aacagtattc
    46741 atttgatcca atcagcccgg atgctgtagt cacaaccaat atcgcatggc aatacagcga
    46801 cgttaatgtc gttgcagtgc atcacgcctt actgaccagt ggtctgccgg taagcgaagt
    46861 ggatattgtt tgcacacttc ctctgacaga gtattacgac agaaataacc aacccaatac
    46921 ggaaaatatt gagcgtaaga aagcaaactt ccggaaaaaa attacattaa atggcgggga
    46981 tacattcaca ataaaagatg taaaagtcat gcctgaatct ataccggcag gttatgaagt
    47041 tctacaagaa ctggatgagt tagattcttt attaattata gatctcgggg gcaccacatt
    47101 agatatttct caggtaatgg ggaaattatc ggggatcagt aaaatatacg gagactcatc
    47161 tcttggtgtc tctctggtta catctgcagt aaaagatgcc ctttctcttg cgagaacaaa
    47221 aggaagtagc tatcttgctg acgatataat cattcacaga aaagataata actatctgaa
    47281 gcaacgaatt aatgatgaga acaaaatatc aatagtcacc gaagcaatga atgaagcact
    47341 tcgtaaactt gagcaacgtg tattaaatac gctcaatgaa ttttctggtt atactcatgt
    47401 tatggttata ggcggtggcg cagaattaat atgcgatgca gtaaaaaaac acacacagat
    47461 tcgtgatgaa cgttttttca aaaccaataa ctctcaatat gatttagtta acggtatgta
    47521 tctcataggt aattaatgat ggacaagcgc agaaccattg ccttcaaact aaatccagat
    47581 gtaaatcaaa cagataaaat tgtttgtgat acactggaca gtatcccgca aggggaacga
    47641 agccgcctta accgggccgc actgacggca ggtctggcct tatacagaca agatccccgg
    47701 acccctttcc ttttatgtga gctgctgacg aaagaaacca cattttcaga tatcgtgaat
    47761 atattgagat cgctatttcc aaaagagatg gccgatttta attcttcaat agtcactcaa
    47821 tcctcttcac aacaagagca aaaaagtgat gaagagacca aaaaaaatgc gatgaagcta
    47881 ataaattaat tcaattatta ttgagttccc tttatccact atcaggctgg ataaagggaa
    47941 ctcaatcaag ttattttctt accagtcatt acataatcgt tattatgaaa taatcgtttg
    48001 cactgtctct gttattcagg caatttcaat aaaggcactt gctcacgctc tgtcattttc
    48061 tgaaactctt catgctgcat ttcgattttt cttctaaaat cataacttgc atacctgttc
    48121 tttttatgta tatccagatc tatttttgtg acgagttcaa accacaatga tagcactccc
    48181 catgcagcgt tatgaaacat ttccgcaaca gatttagagt ctgtattaac cgcttcagtc
    48241 atgtagtcgc taatacgttc tgcagtgatt ttcgttattt catcgtaatt cattttatcc
    48301 cctacaagac tatttaatgg aatgaaaaat atttggtctg ataacaatgc tcctgaatta
    48361 acacctgcag gctatgttaa agttgccctc acacacaatc tacctgtttt ctcgtgtgtg
    48421 tcacaggcgc aatacataaa tagtctggcc attaagttcg taacggattt cgtactcgcc
    48481 agcaaaaatc cgtctgacat ccctggtttc aaactgaaac aactgttcat cccatacgtg
    48541 gatgagttaa cagaatgacc ggcgcctgtg tcagggacta caccgttttg gctgcagctg
    48601 gtttactgac caacgccaga aaatcatata actgcgtcaa tgtcagcgta tccagcgcga
    48661 ctgtattatt tttctctgca accagaatat tgcattaggt tattaccagg tgttctaatg
    48721 tacaggtaaa gacgttcttt ataatctgaa atctctactt ttaatccctc agactttgcc
    48781 tgctgaaagc cctcgtcaag gtgcgctttt attaggttaa tgtctggatg tcttgtatcg
    48841 ggggcaattc tgtattggtg taacgatggg gtggatgttc cctgcaataa gacctccaga
    48901 tagagattgg cacaaccatt cccattattt ctaatagttt attggtaatc acaaactgta
    48961 tatttagcca ttatattgaa tcctgtgaag taaacggcga cggctcaaaa ttttatcaaa
    49021 cctaagataa ttctaccatc aaagttaata atttgaattt atatgacggc tctatggggg
    49081 ggtaaaaatc cccgtgcaga gaggcgattt tcagagttga aaacgtcgtc gttaagaaca
    49141 ccaaagtcag taccaccaac ccggaaagta gcttcatgca ccgggacaac agcagttttg
    49201 gtgtccgaaa tcgtacaatt ctgtacatct acgcaaggaa atgtacggaa gtaagccaaa
    49261 tggttatcgg acggtcggaa catttcgtac agcctttacg ttcagtgagt tagctggcct
    49321 agatgtaacc actcgttcaa ctcaattctc ttagcgtggg aaaatttccg ttttccaagc
    49381 ggccccctca agcggacccc ttatagtaca ataattttcg atatccaaac tgcccccaat
    49441 tacatctcca gtctctggca gcagagatca tatatttcca ttgccgccgt agccacgata
    49501 ttctggctat tcacaaaaac aatttagccc caaagcgcag taaaaaagta cactatgaag
    49561 gtgtatgata ccttcatagt gcttaagtaa ttatgagtaa tataatctgt gtaaactact
    49621 aattgcttca ccgtcagtga ttaaatgcgt agctgttcct tgtcttatat aatacctatt
    49681 ttcaaaatga cagacttctc cgacactttt tatttcgatt tttaaaacat gcagtccttc
    49741 atatgaacat aatgaaatat cttttagtat ttgttgtttc aaagcctcat taaaattaaa
    49801 tgattctatt ttttgtttta tgaacagaaa gtaattatct atgttttttg attgaattac
    49861 agcttcatga tcaattccat tgatataata gccattgcac tcaatagggg taacaccata
    49921 taaagtctta actctgtctg ccgtagcttt ggtatcggaa acaccaacta aaatataacc
    49981 attagattct ctaccgatgt tatttatggc gacagccgtt tggactatat taatgaaaca
    50041 ttcttcgtct attttattct cgcctgagag aataaataat ccctgtttgg taatgactcc
    50101 aacttattga tagtgtttta tgttcagata atgcccgatg actttgtcat gcagctccac
    50161 cgattttgag aacgacagcg acttccgtcc cagccgtgcc aggtgctgcc tcagattcag
    50221 gttatgccgc tcaattcgct gcgtatatcg cttgctgatt acgtgcagct ttcccttcag
    50281 gcgggattca tacagcggcc agccatccgt catccatatc accacgtcaa agggtgacag
    50341 caggctcata agacgcccca gcgtcgccat agtgcgttca ccgaatacgt gcgcaacaac
    50401 cgtcttccgg agcctgtcat acgcgtaaaa cagccagcgc tggcgcgatt tagccccgac
    50461 gtatccccac tgttcgtcca tttccgcgca gacgatgacg tcactgcccg gctgtatgcg
    50521 cgaggttacc gactgcggcc tgagtttttt aaatggcgga aaatcgtgtt gaggccaacg
    50581 cccataatgc gggcggttgc ccggcatcca acgccattca tggccatatc aatgattttc
    50641 tggtgcgtac cgggttgaga agcggtgtaa gtgaactgca gttgccatgt tttacggcag
    50701 tgagagcaga gatagcgctg atgtccggc
//
LOCUS       DAZSNM010000007        47295 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZSNM010000007 DAZSNM010000000
VERSION     DAZSNM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47295
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.007"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..470
                     /gene="sseA"
                     /locus_tag="ACHW42_000343"
     CDS             <1..470
                     /gene="sseA"
                     /locus_tag="ACHW42_000343"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR8942317.1"
                     /translation="QRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHENTAQ
                     IIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFFGRGV
                     SYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            1132..1257
                     /gene="timP"
                     /locus_tag="ACHW42_000344"
     CDS             1132..1257
                     /gene="timP"
                     /locus_tag="ACHW42_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR8942318.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(1289..2065)
                     /gene="sseB"
                     /locus_tag="ACHW42_000345"
     CDS             complement(1289..2065)
                     /gene="sseB"
                     /locus_tag="ACHW42_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR8942319.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2207..3490)
                     /gene="pepB"
                     /locus_tag="ACHW42_000346"
     CDS             complement(2207..3490)
                     /gene="pepB"
                     /locus_tag="ACHW42_000346"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR8942320.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3549..3749)
                     /gene="iscX"
                     /locus_tag="ACHW42_000347"
     CDS             complement(3549..3749)
                     /gene="iscX"
                     /locus_tag="ACHW42_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR8942321.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3761..4096)
                     /gene="fdx"
                     /locus_tag="ACHW42_000348"
     CDS             complement(3761..4096)
                     /gene="fdx"
                     /locus_tag="ACHW42_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR8942322.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4098..5948)
                     /gene="hscA"
                     /locus_tag="ACHW42_000349"
     CDS             complement(4098..5948)
                     /gene="hscA"
                     /locus_tag="ACHW42_000349"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR8942323.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5965..6480)
                     /gene="hscB"
                     /locus_tag="ACHW42_000350"
     CDS             complement(5965..6480)
                     /gene="hscB"
                     /locus_tag="ACHW42_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR8942324.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6576..6899)
                     /gene="iscA"
                     /locus_tag="ACHW42_000351"
     CDS             complement(6576..6899)
                     /gene="iscA"
                     /locus_tag="ACHW42_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR8942325.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6916..7302)
                     /gene="iscU"
                     /locus_tag="ACHW42_000352"
     CDS             complement(6916..7302)
                     /gene="iscU"
                     /locus_tag="ACHW42_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR8942326.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7330..8544)
                     /gene="iscS"
                     /locus_tag="ACHW42_000353"
     CDS             complement(7330..8544)
                     /gene="iscS"
                     /locus_tag="ACHW42_000353"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR8942327.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8656..9144)
                     /gene="iscR"
                     /locus_tag="ACHW42_000354"
     CDS             complement(8656..9144)
                     /gene="iscR"
                     /locus_tag="ACHW42_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR8942328.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9414..10154)
                     /gene="trmJ"
                     /locus_tag="ACHW42_000355"
     CDS             complement(9414..10154)
                     /gene="trmJ"
                     /locus_tag="ACHW42_000355"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR8942329.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10273..11076
                     /gene="suhB"
                     /locus_tag="ACHW42_000356"
     CDS             10273..11076
                     /gene="suhB"
                     /locus_tag="ACHW42_000356"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR8942330.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11221..12075
                     /locus_tag="ACHW42_000357"
     CDS             11221..12075
                     /locus_tag="ACHW42_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR8942331.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12266..13546
                     /gene="csiE"
                     /locus_tag="ACHW42_000358"
     CDS             12266..13546
                     /gene="csiE"
                     /locus_tag="ACHW42_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR8942332.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13538..14677)
                     /locus_tag="ACHW42_000359"
     CDS             complement(13538..14677)
                     /locus_tag="ACHW42_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR8942333.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14837..15727)
                     /gene="hcaR"
                     /locus_tag="ACHW42_000360"
     CDS             complement(14837..15727)
                     /gene="hcaR"
                     /locus_tag="ACHW42_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR8942334.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15863..17224
                     /gene="hcaE"
                     /locus_tag="ACHW42_000361"
     CDS             15863..17224
                     /gene="hcaE"
                     /locus_tag="ACHW42_000361"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR8942335.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17221..17739
                     /gene="hcaF"
                     /locus_tag="ACHW42_000362"
     CDS             17221..17739
                     /gene="hcaF"
                     /locus_tag="ACHW42_000362"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR8942336.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17739..18059
                     /locus_tag="ACHW42_000363"
     CDS             17739..18059
                     /locus_tag="ACHW42_000363"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR8942337.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18056..18868
                     /gene="hcaB"
                     /locus_tag="ACHW42_000364"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18056..18868
                     /gene="hcaB"
                     /locus_tag="ACHW42_000364"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR8942338.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18878..20095
                     /gene="hcaD"
                     /locus_tag="ACHW42_000365"
     CDS             18878..20095
                     /gene="hcaD"
                     /locus_tag="ACHW42_000365"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR8942339.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            20192..20614
                     /locus_tag="ACHW42_000366"
     CDS             20192..20614
                     /locus_tag="ACHW42_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR8942340.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20662..21534)
                     /locus_tag="ACHW42_000367"
     CDS             complement(20662..21534)
                     /locus_tag="ACHW42_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR8942341.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21546..22640)
                     /locus_tag="ACHW42_000368"
     CDS             complement(21546..22640)
                     /locus_tag="ACHW42_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR8942342.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22673..23671)
                     /locus_tag="ACHW42_000369"
     CDS             complement(22673..23671)
                     /locus_tag="ACHW42_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8942343.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23696..25207)
                     /locus_tag="ACHW42_000370"
     CDS             complement(23696..25207)
                     /locus_tag="ACHW42_000370"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR8942344.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25230..26212)
                     /locus_tag="ACHW42_000371"
                     /pseudo
     CDS             complement(25230..26212)
                     /locus_tag="ACHW42_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26309..29590)
                     /locus_tag="ACHW42_000372"
                     /pseudo
     CDS             complement(26309..29590)
                     /locus_tag="ACHW42_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            29708..30901
                     /locus_tag="ACHW42_000373"
     CDS             29708..30901
                     /locus_tag="ACHW42_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFR8942345.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31099..32352)
                     /gene="glyA"
                     /locus_tag="ACHW42_000374"
     CDS             complement(31099..32352)
                     /gene="glyA"
                     /locus_tag="ACHW42_000374"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR8942346.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32679..33869
                     /gene="hmpA"
                     /locus_tag="ACHW42_000375"
     CDS             32679..33869
                     /gene="hmpA"
                     /locus_tag="ACHW42_000375"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR8942347.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33914..34252)
                     /gene="glnB"
                     /locus_tag="ACHW42_000376"
     CDS             complement(33914..34252)
                     /gene="glnB"
                     /locus_tag="ACHW42_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR8942348.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34313..35647)
                     /gene="glrR"
                     /locus_tag="ACHW42_000377"
     CDS             complement(34313..35647)
                     /gene="glrR"
                     /locus_tag="ACHW42_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR8942349.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35637..36350)
                     /gene="qseG"
                     /locus_tag="ACHW42_000378"
     CDS             complement(35637..36350)
                     /gene="qseG"
                     /locus_tag="ACHW42_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR8942350.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIECQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36515..37942)
                     /gene="qseE"
                     /locus_tag="ACHW42_000379"
     CDS             complement(36515..37942)
                     /gene="qseE"
                     /locus_tag="ACHW42_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR8942351.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38500..42387)
                     /gene="purL"
                     /locus_tag="ACHW42_000380"
                     /gene_synonym="purI"
     CDS             complement(38500..42387)
                     /gene="purL"
                     /locus_tag="ACHW42_000380"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR8942352.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42646..44202
                     /gene="mltF"
                     /locus_tag="ACHW42_000381"
     CDS             42646..44202
                     /gene="mltF"
                     /locus_tag="ACHW42_000381"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR8942353.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44199..44702)
                     /gene="tadA"
                     /locus_tag="ACHW42_000382"
     CDS             complement(44199..44702)
                     /gene="tadA"
                     /locus_tag="ACHW42_000382"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR8942354.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(44760..45395)
                     /gene="yfhb"
                     /locus_tag="ACHW42_000383"
     CDS             complement(44760..45395)
                     /gene="yfhb"
                     /locus_tag="ACHW42_000383"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR8942355.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45604..46452
                     /locus_tag="ACHW42_000384"
     CDS             45604..46452
                     /locus_tag="ACHW42_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR8942356.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46791..47295)
                     /locus_tag="ACHW42_000385"
                     /pseudo
     CDS             complement(46791..47295)
                     /locus_tag="ACHW42_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11276 a        12959 c        11894 g        11166 t
ORIGIN      
        1 ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa ggagagttta
       61 acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg gcaagccatg
      121 aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca gaagttgatg
      181 aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt ccgtggacgg
      241 aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata ttttttggtc
      301 gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta acggcagccg
      361 tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac gacggcgcat
      421 ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa gtatattaca
      481 ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc gcggcctttc
      541 cagcaggttg acttgtgtta catgagcaac gcaggtgctt cacagcaaaa caatactcac
      601 cagtaactct ctttttgtca agcaaaagag agtaattatt gtttatttag cgtattatcg
      661 acaccggccc tttccgccgt gttcggtaat aaaataacct ggcttattag tccgaattca
      721 gacaaatata aacaaatcct gctcaaaatt aaaaattcta accggtaaaa gatattactt
      781 aaacatgtaa atttactttc ctttaaaaaa caaaaaaccg ccaaaatcag gcggttattt
      841 gttgctggtc cggttcgcgg cctttccagc aggttgtatt accgtagtaa tgcaagcgcg
      901 tctcagcgga gacaatactc gccagtaact ctctttttgt caagcaaaag agagttatta
      961 ttgttctgtt agtgtattat ccactgcggc cctttccgcc gtctcgcaag cgggcgctgg
     1021 cttaaggaaa ggatgttccg cgaccgttag agcaagcgcg tctcagcgct tgctatcgcg
     1081 gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac aatgaagata
     1141 cggtgcttct gtatcgtact tattgtttct ggtgcgctgt tttcccaagt aaataataac
     1201 cggagtctct ccggcgacaa tttactggtg gttaacaacc ttcagagcag caagtaagcc
     1261 cgaatgccgc cctttagggc ggcatatttt agattatccg gttttgttta aagtcacgca
     1321 aaaaaccacc ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata aagtggctaa
     1381 ttcctttttc cccttttttc acctgacaaa tatcgattgg ttcatcgcca ggtaatgtat
     1441 cggtcgctac acttcccgtc gcctgaataa tttcttcgat atcaccatcg gcttcaatgc
     1501 caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata aaagcacgct
     1561 tcaccggctt aatggtttta aataaggtgg tgagtgaatc aatcatttgt gctggcggct
     1621 ctgcgacttc cgataatatc agcgattcac cgccttccag gatttcctgg ctgctcagcg
     1681 gatttccctc ttcaccaatc aacaaactga tttcacgcgg cataaattct ttaccggtcg
     1741 gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat gtgcgaacgg
     1801 gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagcgcttct aacgaggtga
     1861 aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca agcgcactat
     1921 cttcaaccac agcctcgccc tgcgccgccg taccaggcac ccagacggtg gattccagta
     1981 gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc agcaggtctt
     2041 caagttcgtt ttttgtttcg gacataagaa ccacaattca ttcaacgttc ggggcgcaaa
     2101 tgcctgatgc gctacgctta tcaggcctac aagggattcg caatttgttg aatttgcaga
     2161 atttgtaggc cggataaggc gtttacgccg catccggcat attagtttac gccgttaaca
     2221 gattagctat cgtgcgcaca ccaagtcccg tagcgcccgc agaccactgt tcaaccggcg
     2281 ctttacggta agtcgccgag cagtcgatat gcagccagcc ttgctgatag ttctcaacaa
     2341 agtgcgacag gaagcccgcc gccgtgctcg cgcctgccgg atacgccgcg cttccggtat
     2401 tgttcagttc ggcaaagtta gacggcagct ggctgcggtg gaattccgcc agcggcagac
     2461 gccagaatgg ttcgttctct tgtgaggcac tcgccagtaa acgaccggca agcgcatcgt
     2521 caaaactgaa cagcgcgtga taatcattac ccagcgcagt tttcgccgcc ccggtgaggg
     2581 tcgccgcatc aatgatcatt tccggtttct gcgcactggc atcaatcaga ccatcggcaa
     2641 gcaccagacg cccttcggca tcagtgttca tcacttcaac ttttttaccg ttgcgatagg
     2701 tgatgatatc gcccagcttg aacgcattgc cgctaatcag gttatccgca cagcagagga
     2761 acagcttcac gcgcttgttc agtccgcgca taatggcaaa tgccagcgcc ccggtaaccg
     2821 ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa cgcagtctgt ttgatgctgt
     2881 agccgccgga gtcaaaagtg atacctttac ctaccaggca cgcgtacacc ggtgcttctt
     2941 tatcgccagt tgggttgtaa tccagcgcca gcaataccgg agaacgttct gaaccgcgcc
     3001 cgacagtgtg cagccccata taaccttgct cacgcagatc ttcgcctttg gtgatccgat
     3061 aagtcacacg atcgcccgcg acgttgctga tcagatcaac agcacgctgt gccagttgcg
     3121 atggtcccaa ttcttctgcc ggtgcgttga tggtgtcacg cacccagtcg atgatcatca
     3181 ggcggttatc cagttcctgg cgctgggcat cgtccagatc cggccactcc actttacgcg
     3241 tgcctttcgg gcctttataa ccttgccaga atgcccagca gcgatccgca tcccagcctt
     3301 caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat cttgcgggcc gcacgctgga
     3361 tcagccccag atcgtctgcc ccgttcaaat gcagggtaat gccgtcatta ttaatgctgt
     3421 aagttgcttt ttctccccag cgcgcgtcgg caggttgggt agagagggta atcttcatcg
     3481 cttctgtcat tttagttatc cttattcgtt taacagcaaa acgggctacc cccaggcagc
     3541 ccgttcattc actcggcttc gtccagccag actaacaaaa tcgcttcgag gattttctcg
     3601 ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg atgcatatcg
     3661 gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc ttcgccaatt
     3721 tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca cgcgcatggt
     3781 tgatagtgta acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc gcctggcagc
     3841 ttaaacggct ttccggctcc agtccccagg ctttgtccag catgtcgtct tcctgctctg
     3901 agctttccgg cagtgagtca aaaccttcac gaacgatgca gtggcaggtg gtgcaagcac
     3961 aggatttttc acaggcgtgt tcaatctcaa taccgttacg cagcgctgcg tcgagaatgg
     4021 tttcaccgct attagcttcc agaacagcgc catcagggca gaggtcctga tgaggcaaaa
     4081 taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc acgacgaacc
     4141 gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt tttaatcgct
     4201 tgttcgatgg cgtcaacatc atcgccctgc gccacttcac tcaggtgagc ggcagcattg
     4261 tcaatgacct gacgctctgc ggcgcttaac agcgcggcat cagcagccag cgcgccgtgc
     4321 agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcatacg ggcttttacg
     4381 tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct atcggtcaga
     4441 ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc catcgccgtc
     4501 acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc accgccagcc
     4561 ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc ctgcaccagt
     4621 tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt aaaggtggtg
     4681 aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt ctccaccagg
     4741 ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag catttcgctg
     4801 tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc gacgacttta
     4861 tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg ttcacgcacc
     4921 agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc agcttctaca
     4981 cccgcgtctt tcagcgcgcg acgacaagcc agtaaggttc gttttaccag tggcgcgatc
     5041 agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt aacggtcacg
     5101 gagtccgcat cgctcagcgc gattttggct gcaatggtgg catccagcag ttcacgctga
     5161 acgcggttat cgctacgatc aggaatgtcc gcctgctcgc gaatgtaatc cgccagcaga
     5221 tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac ttcaaacacg
     5281 ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc ataaacagcg
     5341 atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcagt cggttcgtta
     5401 agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc ctgacgctgg
     5461 gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc tgccagggct
     5521 tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac cgggttcagt
     5581 agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa ttgataaggc
     5641 agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg tttaacagaa
     5701 ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc caccgaatgc
     5761 ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg gtcggctaac
     5821 gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc caggtcaata
     5881 ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact aatttgtaat
     5941 aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga gttgttcggc
     6001 actgctccgc agtttatcga gaaaacgcag cttacgcacc gtatccgccg ccgcgtccca
     6061 cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca tctttttcac
     6121 acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct cgtccagctc
     6181 ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat gctgctcgct
     6241 ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg gatgacgcag
     6301 cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt gttccgccag
     6361 gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa aacgcaggct
     6421 cagtgcctgg gtatcgagtt gataacgggc aggcaagcca aagagggtga agtaatccat
     6481 aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac aaaccccacg
     6541 cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct ttcgccgcaa
     6601 ccacactcat ctttaacgtt cgggttggtg aatttaaacc cttcattcag gccttctttt
     6661 acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac cacgactttc
     6721 acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa ttccagtaca
     6781 taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa ccctttgccg
     6841 cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt aatcgacata
     6901 accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa tggcggcttt
     6961 gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt caagttcttc
     7021 agcaatatcg gtgtttttga tcgcctgcgc ttcgtcgaga gacttccctt tcacccattc
     7081 ggtgaccagg gagctggaag cgatagcgga accgcagccg taagttttaa aacgcgcgtc
     7141 ttcaatgata ccttcatcgt tgactttaat ctgcaacttc attacgtcgc cacaggccgg
     7201 tgcacccacc atgccgctgc cgacgttctc gtcgttgtta tcaaaggaac ccacgttacg
     7261 cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt ctcctgattc
     7321 cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct gcttgtacat
     7381 ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta actcgatggt
     7441 gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag agctatgtgc
     7501 cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc ttgctgacgt
     7561 acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg actcaccttc
     7621 aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt caccgttcag
     7681 gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc cgcgcagacg
     7741 ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcttcgccca ttccgacgat
     7801 ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc cgtgcatttg
     7861 cgcttcgatg cgtacacgcg gtttacgacg cacatacagc gcaccgatac ctttcgggcc
     7921 atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca ggtcgattgg
     7981 cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag cacggcacat
     8041 ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt gcatgatgga
     8101 aacaaggatg gtgtcgtcac gcatcgccgc ttcgagttct ttcaggtcga taataccgtt
     8161 acgctgcggt gccaggtagg tgacttcgaa accttcgcgc tccagctgac ggcaagtatc
     8221 cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt tctgataaaa
     8281 gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg taaagacgat
     8341 ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat ctaccgcttc
     8401 ttcagcctgc cagccgaaac ggtgagaacg agaggccggg ttaccaaagg ttccgtccat
     8461 cgtcatgaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg ttgcggagta
     8521 gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc aatgcaagga
     8581 atcaggctac cggctggatg tacgaccgtg tttacggagt atttagcact ccggcctgat
     8641 tctgaattct ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg tgcgggtgcg
     8701 tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt tattaaccag
     8761 ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat cacgccacag
     8821 cgcgtgggtc aggcatttat cgccgccctg gcagccgcct ttaccctgac aacgggtggc
     8881 atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc tggcatcttt
     8941 gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat ttttacgcag
     9001 acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg aaatatcagc
     9061 caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg tcacggcata
     9121 gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc tgcccggggt
     9181 tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt cccgagtaaa
     9241 ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg ccattttttt
     9301 atcgtggcgt ttgtacctgt tgtcggatgc ggtgtgaaca ccttatccga cctacgattc
     9361 tgtacattgt aggcctgata agacgcgtta agcgtcgcat caggcaacca ctgttattcg
     9421 gccttgttac ctttattctg ctgctcaata gaagccagaa tcccgcgcag gatattcaat
     9481 tcctggcttt ccgggcgcgc acgggtaaac agacggcgca atttattcat cacctgcccc
     9541 ggatggtttt cacggataaa accggttgcc agcagcgttt gttccagatg accataaaaa
     9601 cgctccagat catcgaccag cggatacggc gtctcttcat gctcgacctg ctcgccattt
     9661 tcctgagttg ccagccaggc catacgcact tcataggcaa taacctgaac cgccattgcc
     9721 aggttcagcg aactgtattc cgggtttgcc gcaatcgcga catgataatg gcatttctgc
     9781 aactcttcat tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac cggggtattt
     9841 gccgcttcag cgacgctttt cagaccgcat tcgcgcgggt cgagcatcgg ccacggcagc
     9901 gtgcgggagc gtgcgctggt gcccaccacc aggctacaac cggctaacgc ttcgtcgagc
     9961 gtatcgacga tgtgagcatt accaatcaca tcgctggccc ctgcagccag ggcaatcgcc
    10021 tgggagtcgg gtttcaccag tggattaacc agccacagat tggttaatcc cattgttttc
    10081 atggcacggg caacagaacc catattgccg gtgtgtgacg tctccaccag cacaattcga
    10141 atattttgca gcattgtctt tcttcatcta aagattattc acgcatctta tcataaaacg
    10201 aagacagatg ccgatctcgc tgctatactc tgcgccgttt tcccgttctt taacatccag
    10261 tgagagagac cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg caaggcgggt
    10321 aatttaattg ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca gaaaggcagt
    10381 aacgatttcg tgaccaacgt agataaagct gccgaagcgg tgattatcga cacgattcgt
    10441 aaatcttacc cacagcacac catcatcacc gaagaaagcg gtgaacttga aggtactgat
    10501 caggatgttc aatgggttat cgatccactg gatggtacta ccaactttat caaacgtctg
    10561 ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt tgctgtggta
    10621 tacgatccta tgcgtaacga actgttcacc gccactcgcg gtcaaggcgc acagctgaac
    10681 ggctaccgtc tgcgcggtag caccgctcgc gatctcgacg gtaccattct ggcgaccggc
    10741 ttcccgttca aagcaaaaca gtacgccact acctacatca acatcgtcgg caagctgttc
    10801 aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc tggatctggc ttacgtcgct
    10861 gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt cgcggcaggc
    10921 gagctgctgg ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg tcataactat
    10981 atgctgaccg gtaacatcgt tgctggtaac ccgcgtgttg tgaaagccat actggcgaac
    11041 atgcgtgacg agttaagcga cgctctgaag cattaatgac tcaggcgggt gatatcactc
    11101 acccgccctc gcctttcagg cgctattccg aaatacttcc tcaccgcttt actttctttc
    11161 gtcactctcc caccatcttt ccccgattaa tggatgataa agaagtcgca aggaaaggtt
    11221 atggcactgc cagtgaacaa acgcgttccc aaaattctgt ttattctctt tgttgttgcc
    11281 ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct tctattatcc tgacgataaa
    11341 atttacggtc ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa ggacggtact
    11401 cgtctgcaag gctggtttat tccttcttcg acgggccctg ctgacaacgc catcgcaacc
    11461 atcattcatg ctcacggcaa tgccggaaat atgtccgccc actggccgct ggtcagttgg
    11521 ttacctgagc gtaatttcaa cgtttttatg tttgattatc gcgggtttgg taaatcaaaa
    11581 ggcactccgt cccaggccgg attgctggac gatacgcaaa gcgccatcaa tgtggtgcgc
    11641 catcgcagtg atgtaaaccc acaacagctg gtgctgttcg ggcagagtat tggcggggcg
    11701 aatattcttg ctgttattgg tcagggtgat cgtgaaggca tacgtgcggt gatcctcgac
    11761 tccacatttg cctcttatgc aaccatcgcc aaccagatga tccccggcag tggctactta
    11821 cttgatgaga gttacagcgg cgaaaattac atcgccagcg tcagcccgat cccgctttta
    11881 ctcattcacg gtaaagccga tcacgttatc ccatggcagc acagcgaaaa gttgtatagc
    11941 ctggcaaaag agccaaaacg gttgatccta atcccggatg gcgaacacat tgatgctttt
    12001 tccgatcgtc acggcaatgt ttatcgcgaa cagatggtgg actttatcct tagcgcgttg
    12061 aatccgcaga actaacccat gatcgctagc acgataatca ttcacaaaac caccttaaga
    12121 catgctaatc cactggtcag aacagtttaa aatgagaaaa attctgtgac gcttgccaac
    12181 atttctgatg attagcattc ccttcgccat ttccctgagc aaactttagc tattcttatc
    12241 aattatgctt atgggagatc tacagatgat gcctacgctt gctccaccat ctgtcctttc
    12301 ggctccccag cgccgctgcc agatcttgct gacgctcttt cagccggggt tgaccgccac
    12361 catggcaacc ttcagcgagc ttaatggtgt ggatgatgat attgccagtc ttgatatcag
    12421 cgaaacagga cgggagatcc tgcgctatca tcaactcaca ctgacgactg gttatgacgg
    12481 tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc actggctacg
    12541 acgtggtttc cgtctgtgtc cgtcatttat taccagccaa ttcacccccg ccctgaagag
    12601 tgaactgaag cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc tacaagcgtt
    12661 agtgaatctc tgctcccgac ggctgcaaaa acgctttgaa tcgcgcgata ttcatttcct
    12721 gtgtctgtat ctgcaatatt gtttgctgca gcaccacgct ggaattacgc ctcagtttaa
    12781 tccgctccaa cgtcgctggg cggaatcctg ccttgaattt caggtagcgc aggaaattgg
    12841 acgccactgg cagcgtcggg cgctccagca tgtaccgcct gatgagccac tgtttatggc
    12901 attacttttt tccatgttgc gggttcccga tccactgcgg gatgcgcatc agcgggacag
    12961 acaattgcgt cagtctatca aacgtctggt aaaccatttt cgtgagctgg gaaatgttcg
    13021 tttttatgat gaacaggggt tatgtgatca gctttatatc cacctcgccc aggcgttaaa
    13081 tcgcagtttg tttgccatcg gtattgataa taccctgccg gaagagttcg ccagactgta
    13141 tccacgcctg gtacgcacca cccgcgcggc gctggccgga tttgaaagtg aatacggcgt
    13201 ccatctttct gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct ggctaatgca
    13261 ggaaaacgat ctgcatgaaa aacagattat tctactgacc gggaatgata gcgagcgaga
    13321 agcgcagatt gagcagcagt tacgcgaact aacgttactg ccgctcaaca ttaagcatat
    13381 gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc gcggcactga ttattgcgcc
    13441 ttataccatg ccgttaccgc tcttttcacc accgctgatc tatacggacc tgacgctgac
    13501 aacacatcaa caggagcaga tccgcaaaat gctcgaatca gcatgaggga acaactttcg
    13561 ggcgcaaaaa cattgccgga agcgccacca gcgccattac ccagaacacg ccgtggccca
    13621 gatattgata caggaaaccg gcgaaaacgg tcatgatagc gatactgccg cccatcgcga
    13681 cggcagagta aaccgcctgt aaacggatga cttcgctaac ctggcgagca gcaatataac
    13741 gcatggcggc caggtggcag accgtgaagg tgccgcaatg cagaatttgc accactatca
    13801 accacggcaa cgccgtagtt gctcccataa tgccccagcg cactacgccg caaatcgccg
    13861 agatcaacag catatcgcga gcactacaac ggcggaaaag tttattactc agcgcaaaga
    13921 taatgacttc cgccaccacg cccagcgacc acaaataccc caccgccgag gccgagtagc
    13981 cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc ccctgcaata
    14041 aacaaacgca ggccagaaag cgccagttct ggcgaaccag cgccaaccac gcagaccaac
    14101 cggtgctctc ctgctggcgg cttgcccctt gtggctgaat cgtcggacgg atgagaaagc
    14161 cgagcagcat ggatgccacg cccaacgtca acagcacgag gatcacccga taatcaaaca
    14221 tagtgaccag tttgcccgtc agcgccgagc caatgacaaa cgccaccgag ccccacagtc
    14281 gcactttgcc gtaatcaagc gggaactgct tttgccacgt attcgccagt gcatcggtca
    14341 acggtaccag cggtgagaaa aagaggttaa agccaatcat caccagcatc agccacgcta
    14401 cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgg cagcagtgcc agcacgcgca
    14461 aggcggaaat caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa ctcccgagaa
    14521 aacgggcaac cagacctgcc cccaataaca ggccgatggt ttctggcgtt aaaccaatcc
    14581 ctttaagcca gacgctccag aaaggtaaaa aaatgccgta actaaaaaag tatgtgaaat
    14641 agccgagcgc caaccagcgc gtggattgca aaaccatgag tccctcccgt caggaggcgt
    14701 tagtctgggg ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc gagcctgctt
    14761 cccgatgtct gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg acgcatgttg
    14821 caaaaataat gcgtaattat gccgttacgc ttgccaaacg ttcctgcaca atggcgatga
    14881 aatcgcgtaa tgccagcttc atctcccctt tcttccacgc catcagtaaa gcaatggaag
    14941 gcacatttcc ggcaatggga cgaaaaacaa cctgcccggt attaaaatta ttcatataac
    15001 cgggtatcaa agtgacgccc agccccatgc ccaccagatt catggtcacc agaatattcg
    15061 ttgccacctg gacgatattt ggctggctgt tttcttgcgc aaaccacgct ttaacgatcg
    15121 gcgcaagcga accggaatac gccggatcgg tactgacgaa attcacgcca tccagttgcg
    15181 cggcggtgat ctctttttca tgtgctaaag ggtgatcaac cggtaacacc accactaatg
    15241 gttcgtcaaa aagctccaga taatcaatct ccgggctata aacaggatgg cgcattaagc
    15301 cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt aaactcacca
    15361 gctcaatcaa ggtgtctggc tgtctgagac gaaacatcgg taatactttt ggcaataaat
    15421 tcacttccgc cgatggcaca aagccaatgg ttaattgtct gtcttcctga acaattttcc
    15481 gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc tggagaaaac
    15541 attctcccgc cgccgtcaac gcgactttgc gcttatccct caccagtaac ggaacaccga
    15601 cacagttttc aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta tgcagttttt
    15661 ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt aaatgccgta
    15721 gttccatcac cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa ttaactattt
    15781 cacatattag caaccaacca gcaacatcct tatggcacaa aaatagaagg tcaatacatc
    15841 ttatctttca ggattaaaaa atatgaccac accctcagat ttgaacattt accaactgat
    15901 tgacacccaa aacggtcggg tcactccgcg tatttatacc gacccggaca tttaccaact
    15961 ggagcttgag cgtattttcg gtcgttgctg gctatttctc gcccacgaaa gccagatccc
    16021 aaaacccggt gatttcttta acacctacat gggagaagat gcagttgtcg tggtgcgcca
    16081 gaaagacggc agcatcaagg cgtttctcaa ccaatgccgc caccgggcca tgcgtgtgag
    16141 ttatgcagat tgtggcaaca gtcgcgcctt tacctgcccg tatcacggct ggtcttatgg
    16201 cattaacggc gagttgatcg atgtaccgct ggaacctcgc gcctacccac aagggttgtg
    16261 taaatcccac tgggggctaa acgaagttcc ttgtgtggag agttataaag gactgatttt
    16321 tggcaactgg gataccagcg caccgggcct gcatgattac ctgggtgaca ttgcctggta
    16381 tctggatggc atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg gcgtacaaaa
    16441 gtgggtgatc aactgtaact ggaaattccc ggcagagcag ttcgccagtg accagtatca
    16501 tgctctgttc agccatgctt ctgccgttca ggtattaggg gcgaaagatg atggcagcga
    16561 taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca aagatgcgct
    16621 gcaatttggt caggacggtc acggtagcgg tttcttcttt actgaaaaac cggatgctaa
    16681 tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa acctatgccg aagcagaaca
    16741 acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat aacaatatat tccccacgct
    16801 ttcatggcta aacggcactg ccacactccg cgtctggcat ccgcgcggcc ctgatcaagt
    16861 tgaagtgtgg gcgttctgta ttactgacaa agccgcctcc gatgaagtta aagccgcttt
    16921 tgaaaacagc gccactcgtg cttttggtcc tgctggtttt ctcgagcagg atgactcgga
    16981 gaactggtgt gaaatccaga aattgcttaa aggccaccgc gcccgcaaca gcaaactgtg
    17041 tctggaaatg gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg gcattactaa
    17101 ctatatcttt tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg atcttctgag
    17161 tagcgaaagc tggcaggaag tgctcgataa aaccgccgct taccagcagg aggtgatgaa
    17221 atgagtgcgc aagtttcact ggagttacat caccgcatta gccagtttct ctttcacgaa
    17281 gccagtctgc tggatgactg gaaatttcgt gactggttgg cacagctcga cgaagagatt
    17341 cgttacacca tgcgcaccac ggttaacgcg caaacacgcg accgtcgcaa aggcgtccag
    17401 ccgccgacga cctggatttt caatgacacc aaagaccagc tggagcggcg aatcgcccgt
    17461 ctggaaacgg gcatggcctg ggcagaagag ccgccgtcac gcactcgtca cttaatcagc
    17521 aactgccagg taagcgaaac cgacatccca aacgtatttg ccgtgagggt gaattatcta
    17581 ctttatcggg cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg tttcgacaaa
    17641 gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt cctggatcaa
    17701 gcggtgatca cttcccataa cctgagtgta ctgttctgat gaatcgaatt tatgcgtgtc
    17761 ccgttgcgga tgtgccggag ggtgaggctc tccggataga tacctcgccc gtcatcgcct
    17821 tgttcaacgt gggcggcgag ttttatgcca ttaacgatcg ttgcagccat ggtaatgcgt
    17881 cgatgtcaga agggtatctg gaagatgacg ccacggtgga gtgcccgcta cacgccgcca
    17941 gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc caccgatccg ctcaccactt
    18001 atccagtaca cgttgaaggt ggtgacattt tcatcgactt accggaggcg cagccatgag
    18061 cgatctgcat aacgagtcca tttttattac cggcggcgga tcgggattag ggctggcgct
    18121 ggtcgagcga tttatcgaag aaggcgcgca agttgccacg ctggaactgt cggcggcaaa
    18181 agtcgccagt ctgcgtcagc gatttggcga acatattctg gcggtggaag gtaacgtgac
    18241 ctgttatgcc gattatcaac gcgcggtcga tcagatcctg actcgttccg gcaagctgga
    18301 ttgttttatc ggcaatgcag gcatctggga tcacaatgcc tcactggtta atactcccgc
    18361 agagacgctc gaaaccggct tccacgagct gtttaacgtc aacgtactcg gttacctgct
    18421 gggcgcaaaa gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga ttttcacact
    18481 gtcaaatgcc gcctggtatc ctggcggcgg tggcccgctg tacaccacca gtaaacatgc
    18541 cgcaaccgga cttattcgcc aactggctta tgaactggca ccgaaagtgc gggtgaatgg
    18601 cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca caggcgctcg ggcaaagtga
    18661 aacctcgata atgcagtctc tgacgccgga gaaaattgcc gccattttac cgctgcaatt
    18721 tttcccgcaa ccggcggatt ttacggggcc gtatgtgatg ttggcatcgc ggcgcaataa
    18781 tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc gcggcattcg
    18841 ccacgtagcg gctgggctgg atctttaagg aagcacgatg aaagaaaaaa cgatcattat
    18901 tgtcggtggc gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc aagggttcac
    18961 cggtgagctg catctgtttt ccgatgagcg acatcttcct tatgaacgac ctccactctc
    19021 gaaatccatg ttgctggaag attccccgca gttacagcag gtgttacccg ctaactggtg
    19081 gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg gtcgcgacac
    19141 acgagagtta gtgttaacca acggcgaaag ctggcactgg gatcagcttt ttatagcaac
    19201 cggcgcggca gctcgaccgc tgccgttgct tgatgcactg ggagaacgct gctttaccct
    19261 acaccatgcc ggtgatgccg ccagactgcg agaagttctg cagcccgaac ggtcagtcgt
    19321 gattgtcggt gccggaacta ttggtctgga actggctgcc agcgccacgc agcgcagcgc
    19381 agcgcagcgc agatgtaagg tgacagtgat tgaactggcg gcaaccgtca tgggccgtaa
    19441 tgcaccaccg cccgtgcaac gctatctttt acagcgccat cagcaggctg gtgtgcgcat
    19501 tctgctcaat aatgccattg aacatgtggt cgatggtgaa aaagtagaac tgacgctgca
    19561 aagtggggaa acgcttcagg ctgatgtggt gatttacggt attggtatca gcgccaacga
    19621 gcaactggct cgcgaggcca accttgatac tgccaatggc attgtcattg atgaggcttg
    19681 ccgcacctgc gatcccgcga tctttgccgg tggcgatgtg gcaatcactc gtcttgataa
    19741 tggtgcacta caccgctgcg aaagctggga aaacgccaat aaccaggcgc aaattgccgc
    19801 tgccgcaatg ttagggctac cgctaccgct actgccgccg ccgtggttct ggagcgatca
    19861 gtacagtgat aacttacagt ttattggcga tatgcgtggc gatgactggc tttgtcgtgg
    19921 caacccggaa actcagaagg cgatttggtt taatctgcaa aacggcgtgc ttatcggtgc
    19981 ggtcacgctg aatcagggcc gtgagattcg cccaattcgc aaatggatcc agagcggcaa
    20041 aacgtttgat gcgaaactgc tgatagatga gaacatcgcg cttaaatcac tgtaatcagg
    20101 ataattagcg aatatctcaa tgcctggggc gtggcgaggt gcaagagtgt gtattacgtt
    20161 taaatcacat tatcttgcaa agggattggt tatgaacaca ctacgttatt ttgattttgg
    20221 agctgcccgc cccgttttgt tattaattgc ccgtatcgcc gtggtcttaa ttttcattat
    20281 ttttggtttt cccaaaatga tgggctttga cggtacggtc caatatatgg cctcgttggg
    20341 cgcgccaatg ccgatgctgg cagcgattat tgcggtagtt atggaagtgc ccgccgcgat
    20401 attaatcgtg cttggctttt tcacccgtcc gctggcggtg ctgtttattt tctacacgct
    20461 gggtacggcg gtgattggtc accattactg ggatatgacc ggcgatgcgg ttgggccaaa
    20521 tatgattaat ttctggaaga atgtcagtat cgctggcgcg ttcttgctat tggcaattac
    20581 cggaccgggg gcaatttctc tcgatcggcg ttaggaaaga tgccggatgc ggcgtgaacg
    20641 ccttatccgg cattttataa attacagcca ctctacccgc aacgacatct ctgaggttgt
    20701 tgattcccct ggtgctagcg caatgagatc acccccttcc gggcgatgat gctcatccgg
    20761 cgcatggctc atcggttcca gacagaagaa atcaaacgca tatcctttat caaacgcagg
    20821 gtcggaaaca aagatgaaat aacacggtgc aggtggcgtc gtttccatga tgatggcata
    20881 accttcctgc ggctgctcga tgcgggcctg accattccat ccggcaaaac cattgttcac
    20941 ccactgacgc ggcaacggcg cgagctggtt aaaatccagt tcctgcggta gctgctcgca
    21001 aaactcaccc gccagccact gctcccgttc cagccagtaa ccgctcgcct gcgcctgaat
    21061 ccgcgtttgt ggtgacaacg gaaaataagg atgccagccg gtaccaaatg gcagcgtctc
    21121 tgccccttga ttggtgacag agagcgtcac cgtcagcgta tccgccgtta aatgaaacgc
    21181 ctgacttacc cgatagtgat agacaccgct gcgatgttca tacaccagac acaaactatc
    21241 atcgctgtgc gagacacatt gccactcgcc cagccagcca tcgccgtgta gatagtgcgc
    21301 atcccactca acattcggtt gcagttgata ctcacgcccc tgccagacaa aacggttgcc
    21361 gctcacccgg tttgcaaatg gcaccagcgg aaaacatgag gcatcggttg ccacaccgct
    21421 ttttttacca ggacgtaaga gcggcgtcgt atcgcgccag aagccttcga ttacgccgcc
    21481 ctggtcagaa acgtccagct ttagcgaccc gtgggataag gtatagatgg tcatccgcta
    21541 actccttaat ccgggaagtt aatcacaact ttcccgcatt tgccgctcgc catcagcaca
    21601 taggcatcac ctgcctgttc tagcgagaag cgatgggtaa tggcgttacg cggccacagc
    21661 ttccagtcag tcaaatcgtg ggcgcatttt tccatatgga acagactggt cacccaggag
    21721 ccgataatcc gccgttgatg gtgcatcaga tcagcgctga cctcgaattc cacttttccg
    21781 gtttcaccaa tgtaaaccac ccgcccccag tcagcagtgg attgcagtgc cagcaagcga
    21841 cctgcggcat taccggaaca atcgagcgca acatccgcgc caccgtgggt aagttcagca
    21901 attatctgcg gcagaccttc ggtggttgct aaatagccgt gatccatccc ccctaactgt
    21961 tttgccatcg ccagacgttc cggcagcata tcaacgccga tgatccgttt tgcaccgcga
    22021 cctttcgcca gcatcatcgc catcatgccg accggcccca gaccgaccac cagcacgtta
    22081 tcgctgccgg aaacttcgcc gcgcaaaatc ccttcatagg ccgtgccaac gccgcaactg
    22141 ataaacgcac catcttcgta gctcagcgca tccggcagga ggatcagatc tttttcttcc
    22201 gccagcagat attcggcatg accgccgtca cgctgccagc cgtaagccgc ttttcctttg
    22261 ccagtacaag agataggaaa gccgcggcgg cagttcgggc aaaaaccaca gccagaaatg
    22321 tgatacacca gcacgcggtc gccctcttta aaatggcggc agccttgccc catcgccaca
    22381 atctgcccgc acggttcatg accgttgata aatccctggt ataacggttt atcgggtgcc
    22441 gccgccgtgg cacggtgttg atgatagata tagtggacat cgcttccgca aatcccggag
    22501 gatttcattt tgatcagtac ctggttaatc cccggcgtcg gcaccgcaac ttcccgcaga
    22561 tcgacggttg aatttcctag taaataagca gccagcatcg ttttcataaa tccctcatta
    22621 acaatacgat taattttcat ccctgcagca cccgcgccag gggcgttaag gtttagcgtt
    22681 tcgctttact gcttcgctgg gtcagcaaga tattcgccag caccgccacc acgatgatga
    22741 cgccgcgtac cacctgctgg aaaaaggagt taataccgag cagcaccaga ccgttaccga
    22801 ttagcgtaat caccagcaca ccaagcaatg taccgaacag ggaaccgcga ccgccggaaa
    22861 gtgccgtacc gccgaccacg accgcggcga tgacgtcaaa ctccagaccg tttgcggcac
    22921 ctgcgttacc ggaaccgagg cgtgccgcca acaaaatgcc ggtcaccgcc gctaataatc
    22981 ccgaaagggt aaagataaga atgcgcactc gacgaacgtt aataccgcac aactgcgccg
    23041 ccgtggcatt accgccaacg gcaaaaaccg agcgcccgaa ggcggttttt cggctaatga
    23101 acacaaacag cgcaaacaac accatcatga tcagcgcgga tactggcaca ccgagaaatt
    23161 gtccgcccag ccagtccagc acctcgtttt cgtcaatcgg cactggcagt gcgttggtca
    23221 taaacagccc cattccgcgc agagcgctcc acagccccag tgtggcaacg aaacttggta
    23281 cgttaaacac gccgcgcagc accccggcga gcgttcccat cagcgcgcct aacagcaaca
    23341 ccagcagaca agcaatcgcc aacggaactt caaattgcag caaaaatgcc aggcacaccg
    23401 agacaaaagc caccatcggc ccaacgctga catcaatttc accggagata ataatcagcg
    23461 tcatcgccca ggcggcaatc ccaatggtgg cggcatcgcg cagcacgttc atctggttat
    23521 tcaatgagat aaagccaggc gcgttcaggg agaagaccag ataaagaatg gcaatcacca
    23581 ccagcaaacc gatctcatta atatggcgac tgacaaattg tttgagcgag acgctcttgc
    23641 cctgcggcaa tggtaatgac gaagcataca tcttcaggtt cctcggtaaa attcatcagt
    23701 gcacagacag aatggcggac atcagctcat ccacattgac cggcgagtga aactcctgcg
    23761 agaacgtgcc gtgctgtaat aacaggatgc ggtcacacac cagcggcaat tcctccactt
    23821 cactggagat aaacaccacg ctttttcctt cggcagccag ctcacggaca atacggtaaa
    23881 tctgctgttt ggcttcgata tcgacgccgc gcgttggctc gtcgagcaac aaaatctggc
    23941 tggcggcata gacccaacga ccgatcacca ctttttgctg attgccacca gaaagcgtgc
    24001 cgatgggtgt ttcgctactg gcagccttga ccgtcatccg ctgcatcacc tcttcggtca
    24061 ggcggcggat ggtggaccat tgcagcacac cgttggtgct gattttttgc cgattggtca
    24121 gcactgtatt ttcgtcaacg cctagccagg gaatgatccc cgcttctttg cggttttctg
    24181 gcgtatagcc aatgccgcgt ttcagcatgt cgccgtaatc ggggcgcatg attttctcgc
    24241 cgttgataac aatttcgccc tgttcatacg tctccagccc aacaatcgcc ttcagcaatt
    24301 cactgcgccc tgcccccagc aggccagcaa tgccaagcac ttcgccacga cgtagcgtaa
    24361 agctgatatc ctccagcttg ggcttatggc gtaacgcacg gacttccagt acggcctgat
    24421 ccacaatttc ctgaggggca accggcgcaa tatcaacgtg atcacgccct agcattagcg
    24481 acacaatatg atgcgtggag gtgttttcga gcatcacatc gcccgccacc tgaccatcgc
    24541 gcataacggt ggcataggag gcaatgcggc ggatttcttc catccggtgg ctgacataaa
    24601 tcaccgccac gcccagtgcc gacatctttt tcaccgcact gatcaccagt tcaacttctg
    24661 cactggcaag cgagctggtg ggttcatcaa gaatgaccac gcgcggctcg cccttcatca
    24721 cccgtgcaat ttccaccagt tgcttttgcg ccgggcttag cgttgaaaca agttgttcag
    24781 gactaacgtc aacgcccagc gcctgtaagc aacgttgggc atcctgcgcc atttgcaggt
    24841 aatcaatcat gccgttgcgg cgaggccatt gaccaaggca gaggttttct gccaccgtca
    24901 gcccttccac cagacttaat tcctgataaa ccgcacgaac ccccagttca gcggcacggc
    24961 gagtcaacgt agcttcgtca ccttccagtc gcgtctcgcc aatccagata tcaccgctat
    25021 ccgggcgttc gctgccggta agcattcgaa tgagagtcga tttgcccgcg ccgtttttac
    25081 ctaacagcgc acgaacttcg cctttattga gcgtgaagtt aacgttatcc agcgcaacga
    25141 cgccggggta acgcttattt cctgccacca cttttgctac cgggactgcc tctgttgccg
    25201 tgaacatagg gacctctgcg aatcagcgat tagggcagac catcaacgtg cgttgccagc
    25261 cactgtttac catcttccgt tttggtatag agatcgattg gcacctgaat cactttttca
    25321 ccgtcggctt gtttattgat aaccttcaat gtttgcgcga aaacagcatt gcccattttc
    25381 ttaccggaaa tatccactac cgctttcagc acctgattgt tttccagctc ctgagcaatt
    25441 tcggttgtca tatccgaacc gaaaacagca atttttccgg cctgattttg attacgtacc
    25501 gcttttaccg cgccgagtgt cgcaccgccc gactccccca taatggcgtt gagatccggc
    25561 gtggagataa tcagtttttc accaacggaa atcgctttat ctaaaacagt cccttcctgg
    25621 ttagcgacaa tttgcgcgcc gggaacgcgg gattttaata cttcttcaaa tcctttacgt
    25681 cgctgcacac aaacttcaaa ggcttcgcaa ttgatgacgg caattttcgg ctggtcaatt
    25741 ttattggcaa taaaataatc ggcggcagcg ttacccagtt tttaccaaat tccagtggat
    25801 cgccgaccag atacgccgag acatatttat cgacaccctt ttgattaata caggtgttgt
    25861 agcaaatcac cggaataccc gcttcactgg cgcgacgaac ggtacggcta ctgccatttt
    25921 cagacactgc cgataaaata atggcatcga cattacgcgc cacgagggta tcaacaaagg
    25981 tactttcttt cgaaatatcc ccctgggcgt tagtttcaat taactgtacc tgtactgaag
    26041 aatcttttgc cgcatcctga acgccctgac gcactccagc gtaatatccc tgggtatcaa
    26101 ggtatattgc accaatggtc atttctttat ccgcagccct ggcaaatagt gcgctaccta
    26161 atagcgtagc cattaataat agattacggg ttgttctcat ttttttaggc atagtgcctc
    26221 ctgtagggtt tttattaaca acggcttatt ctaattattt tgtgatgagc ggcagcgcgt
    26281 gccgctcaaa tattacagtt aatagggttt agtgaacgta gttaaaaata aaaggcatca
    26341 cagtggtgaa taatgccgct ttcggccagg ccggattaat cgccgtcaat tcagcgcgag
    26401 cagactcata ttgcgcaacc tccccgagtc ccgcggacgc cagcatcgcc accatcaggc
    26461 atttttcttt atgctgctgt tgtaaatcgc catacagcga taacaggtca ggttgtgaaa
    26521 cagcaaagaa atccgcctca atactggttt tcgccatctc ttgcgcccac tgtttcattt
    26581 cactaaacag ttgctgtgcg gtttgctgtt cacccagcag tcgcagcgcc attccttgcc
    26641 agaagagata atcaaccggc tgatcgttgt aataactgtg gatgttaatg gtgcgatcgc
    26701 cggtcgccgc cagacgtaaa caacgcatcg cttcagtttc atcgccctgc gcgttggcgc
    26761 atatcgcctg ccagaaccag atgtcgttat cagtttgccc cggtaaacgg ccttcgctta
    26821 aattctccgg ataatacagc gcggcatgaa gcagttcgct ggcctgctgc ggctctctgg
    26881 catcaagatg ctgccaggcg cgtaataact ggttgaggat aaactgactg gtgaccttcc
    26941 cttccccgcc ttcccacggg tggaatttac gcgtggcgag aatgtccgcc gctttgtctg
    27001 cctgacccgt gagatgccac aaattgagca gttctgcggt catgtcgtcg cgtttcagcg
    27061 caatttccag attattttcc agacgcgcca gtcgtttctc cggtgtggct ccacttaact
    27121 tatccagcaa atcccgttcg aaaagcagac gtgcatcctg cggcgcaagc tgataagcat
    27181 tatcaagata acgcgcggcc agctcataat cgtgtttctt attccacgca tggatcgcta
    27241 acccgcgcca gccgtcggca aactccggcg acatctctac gcaacgttgc cataaggcaa
    27301 tggctttgtt gtagctacgt ttgttgtagt agaagcaggc cagtaaatgg cgagcaaacc
    27361 aacactcttc aatactctcc agcgccgcca cttcttccag cgtattcggg aaacggacaa
    27421 actgcgggaa gacatcaatg gcttttgcga ccagttcgcc acgttcggct ttcggcagca
    27481 agctggcttg caggtaaagc ggcagcgtgc gctggcagtc cagtgcgttc agcatctctg
    27541 ccgccagggt gggcattccc cagttaatca gttgcccggc ggtcatcaga gcgttaacgt
    27601 cgcgtccctg acacagaccg cgccactgcg cgagggctga ttcgctacga ccatcgaacc
    27661 agttcagcca ccacagagtg gcgttcagcg gataatcacg cagcagtttc tcgcgctgca
    27721 aacgcgcgtt gtcctgacga ccacttaaca ccagcagcag attatgcaag caaagcactt
    27781 cctgattggt tgggcaggca cgaagacttt gttggcaaaa atccagaccc gcgtcgaagt
    27841 tatcattacg cgccgccagt cgtgccagac cataatagcc gcctgcttta ctattgccgc
    27901 tccagaccgc acgccagaaa tcctcttcgg cttgttgata ttgtccctga cgttcgtaag
    27961 cactggcgcg aatcaaactt gcttgtccac actgcggttt tttgttcagc gcatgtgcac
    28021 gtttcagcgc ctgactggca tacgccaccg cttgcgggaa atctgcgcgg ttatattcca
    28081 gcatcgccag cgccaggtta cagcgataat ccagcgggtc cagcgccacg ccgcgcaggt
    28141 agtaatcgaa cggtgaacgg ctggcgtgat gatattgctc cagatgctga ccgataaacc
    28201 aggcttcatc tgtactggta atgtcttgtg ctgccagtgg cgctttggcg acgtccggca
    28261 acggcaactc ctgcggctga tgttcctgat aactcagtac aatattgccg tcggcatcgg
    28321 agagttcaat ggtcagtctg tcaggattga taccgtgcaa cacgccctgg atggcggtcg
    28381 ctggtgtcag ggccacggca tcatcgagca acgcgttgca tttgccgatt tcgcggatcg
    28441 ccagacggta tccgttcaac ggagagatgg catacagccc ccactcaatc ccccgctcac
    28501 tacgctggag ttttatcacc gcatcgcggg aggcattttg caccatgccc agagaatgat
    28561 aaggcaggaa atactgctcg aaacgcttct cttcgtaagc atcaagccag gtaaaatcag
    28621 gctggttatc ggcaaaaata ccggtcatca gttcgatata cgggccgtta ttgtcggtca
    28681 gactcttatc ccacgcctgg ccaaattcac tgtgtcccca actccactgt tttttacctg
    28741 gcgcaatatg gtggttggca acgtgcagca aaccgccatc ttcatcgtga caccacgcgc
    28801 cgacaaaatc gtactgcgat ttttcggcca tatatgaggt tggaacaggc acatttttat
    28861 agcgagaaat gtccactccg gcggagtagt ccactttgta gtaagtgccg gtggcgatgg
    28921 ggaaagcgga gacggcccgt ttgccgtgat caaacaccgc agtcacatcc ggtgggaaaa
    28981 cgctctgatg cccttcaccc cctttcactg ccgggttgac ccaccacaag aaatgacgcg
    29041 gcgtggcgtt gccgttatag acgcggctgg cgatttccag tgccgcccgg tcagggcgca
    29101 gggtgaaacc tgtcatcacc tgtaaaccat gcatcggctc cgtttcgccg acccacaccg
    29161 tctgtgcacc gtcttcatgg gcttcgaggg tgaaatcaac gggcataaag gtggtcgggc
    29221 gatggtgttg cggccagtta aactcaatcc cgccggagat ccacggtccc agcatcccca
    29281 ccagcgcagg tttaatgact tcattgtgat agacaaaatc gcgctgtttc actttatccc
    29341 atgcgcgatg cacccgaccg cccagttccg gcaggatcat cactttgatg tagtcgtttt
    29401 ccagccacac cgcctgctag gatttcaggg ttttctgctc gctcagcgta tcggtcacgc
    29461 cgtagggata aaccgcgccg gacgatccct gataaacgcg attttccagg aacatgggat
    29521 gtatatcctg cggcccggtt tcataggtcg ggatctcaac gcgctcttgc cacactttta
    29581 ctggagtcat ggtgccctca atattaacaa gacatactga attaaaagat ttgtggcagt
    29641 gtattgaaca atctggcaat gttttcgcgg aataatcacg caattaacta aacaaggttt
    29701 agtgaagatg agagcctgca ttaacaatca acagattcgc caccataaca aatgcgtgat
    29761 tctggaactg ctgtaccggc aaaagcgcgc caataaatca acgctggccc ggctggcgca
    29821 aatttcgatt ccggcagtca gtaatatttt gcaggaactg gaaagcgaaa aacgggtggt
    29881 gaatattgac gatgaaagcc agacgcgcgg gcatagtagc ggtacatggc tgattgcgcc
    29941 ggaaggtgac tggacgctgt gcctgaacgt gacgcccacc agtattgagt gtcaggttgc
    30001 taatgcttgt ttaagtccga aaggcgaatt tgagtattta cagattgatg taccgacgcc
    30061 gcaggcgctg ctgtccgaaa tcgaaaaatg ctggcatcgc caccgtaaat tgtggccgga
    30121 ccgcaccatc aatctggcgc tggcaatcca cgggcaggtt gatccggtga ccggcgtatc
    30181 gcaaaccatg ccgcaagcgc cgtggacaac gccagttgag gtgaagtatc tgctggaaga
    30241 gaagctgggc attcgggtgc tggtcgataa tgactgcgtg atgctggcgc tggcggagaa
    30301 atggcaaaat aattcgcagg aacgggattt ctgcgtgatc aacgttgatt acggcattgg
    30361 ctcgtcgttc gtgattaacg agcaaattta tcgcggcagt ttgtatggta gcggacagat
    30421 tggtcacacc atcgttaatc cggatggagt cgtctgcgac tgtggacgtt acggcagcct
    30481 ggaaaccgtc gcctcgttaa gtgcattaaa aaaacaggcg cgggtatggc taaaatcaca
    30541 accggttaat actcagcttg agcctgaaaa actgacaacc gcgcagttaa tcgctgcctg
    30601 gcagagtgga gaaccgtgga tcaccagctg ggttgatcgc tctgccaatg ccattggttt
    30661 gagtctgtat aacttcctca acatcctcaa tattaatcag atttggttgt acggtcgcag
    30721 ttgtgccttt ggtgagaact ggcttaatac tattattcgc cagacaggat ttaacccgtt
    30781 cgaccgcgac gaaggaccga gcgtgaaagc gacgcaaatt ggcttcgggc aattaagccg
    30841 cgcacaacag gtgctgggaa ttggctattt gtatgttgag gcgcagttac gacagatttg
    30901 atggcgcgat aacgtagaaa ggcttcccga aggaagcctt gatgatcata aacgaaaaat
    30961 tgcctgatgc gctacgctta tcaggcctac acggagattg caatatattg aatttgcaag
    31021 attttgtagg cctgagaagg tgttcatgcc gcatccaaca tgaacaacga gcacattgtc
    31081 agcaaatcac cgtttcgctt atgcgtaaac cgggtaacgt gcgcagatgt cgagaacttt
    31141 acctttgatg cgctcgataa cggcttcatc attgatgctg tccagcacgt cacacatcca
    31201 gccagccagt tctttcgctt cggcttcttt aaagccgcga cgggtaatcg ccggagtacc
    31261 tacacgaata ccggaggtca caaacgggct cttcggatcg ttcggtacgc tgtttttgtt
    31321 gacggtgatg ttagcacggc ccagagcggc gtctgcttct ttaccggtca ggtttttatc
    31381 aaccagatca accaggaaca ggtggttatc agtgccgccg gaaaccactt tgtagccgcg
    31441 ctcgaggaac acttctacca tcgctttagc gtttttcgcg acctgctgct ggtaagtttt
    31501 gaactcaggc tccatcgctt ctttcagagc aaccgcttta ccggcgatta cgtgcatcaa
    31561 cggaccgccc tgaccaccag ggaaaacggc agagttcagt tttttgtaca gctcttcgct
    31621 accacctttc gccaagatca ggccgccgcg cggacccgcc agggttttgt gagtggtggt
    31681 agtaacaacg tgagcatgag gaaccgggtt cgggtagacg ccagcagcaa ccaggcccgc
    31741 aacgtgcgcc atatcaacga acagataagc accgatgctg tcagcgattt cacgcatttt
    31801 cgcccagtca accacgccgg aatatgcaga gaaaccaccg ataatcattt tcggcttgtg
    31861 ttctttggct tgtttttcca gatcggcgta atcgatatga ccggtagcat cgataccgta
    31921 aggaacgatg ttgtacagtt taccggagaa gttaaccgga gaaccgtgag tcaggtgacc
    31981 gccatgcgcc aggttcatac ccagaacggt atcacctggt tccagcagcg cggtgtagac
    32041 cgcaaagtta gcctgggagc cggagtgcgg ctggacgtta gcgtagtcag cgccgaacag
    32101 ttctttcgca cgatcgatcg ccagttgttc aacgatatca acatactcgc aaccgccgta
    32161 gtagcgtttg cccggataac cttcagcata tttgttggtc agctgagaac cctgcgcctg
    32221 cattacgcgc gggctggtgt agttttcgga ggcgatcagt tcgatgtgct cttcctgacg
    32281 tactttttcc tgctccatag cctgccacag ttcggcatca taatcggcaa tgttcatttc
    32341 acgctttaac atccgcatct cctgactcag ctaacaataa aatttttggc ctttataggc
    32401 ggtcctgttg gacaacggcg aacagtataa ccgaatcatt gtgcgataac aggtcttgac
    32461 aaaggaattt acgcaaacga ttaccttcag gctacgcaag gctttggaga ataaagagct
    32521 tgcaaccgga aacggatttc ttttcaggtt tgtgatgcaa atttttcact tcatcacatt
    32581 ctttctgaaa aacaccaaag aaccatttac attgcagggc tattttttat aagatgcatt
    32641 tgagatacat caattaagat gcaaaaaagg aagaccatat gcttgacgct caaaccatcg
    32701 ctacagtaaa agccaccatc cctttactgg tggaaacggg gccaaagtta accgcccatt
    32761 tctacgaccg tatgtttact cataacccag aactcaaaga aatttttaac atgagtaacc
    32821 agcgtaatgg cgatcaacgt gaagccctgt ttaacgctat tgccgcctac gccagtaata
    32881 ttgaaaacct gcctgcgctg ctgccagcgg tagaaaaaat cgcgcagaag cacaccagct
    32941 tccagatcaa accggaacag tacaacatcg tcggtgaaca cctgttggca acgctggacg
    33001 aaatgttcag cccggggcag gaagtgctgg acgcgtgggg taaagcctat ggtgtactgg
    33061 ctaatgtatt tatcaatcgc gaggcggaaa tctataacga aaacgccagc aaagccggtg
    33121 gttgggaagg tactcgcgat ttccgcattg tggctaaaac accgcgcagc gcgcttatca
    33181 ccagcttcga actggagccg gtcgacggtg gcgcagtggc agaataccgt ccggggcaat
    33241 atctcggcgt ctggctgaag ccggaaggtt tcccgcatca ggaaattcgt cagtactctt
    33301 tgactcgcaa accggatggc aaaggctatc gtatcgcggt gaaacgcgaa gagggtgggc
    33361 aggtatccaa ctggttgcac aatcacgcca atgttggcga tgtcgtgaaa ctggtcgctc
    33421 cggcaggtga tttctttatg gctgtcgcag atgacacacc agtgacgtta atctctaccg
    33481 gtgttggtca aacgccaatg ctggcaatgc tcgacacgct ggcaaaagca ggccacacag
    33541 cacaagtgaa ctggttccat gcggcagaaa atggcgaagt tcacgccttt gctgatgaag
    33601 ttaaggaact ggggcagtca ctgccgcgct ttaccgcgca cacctggtat cgtcagccga
    33661 gcgaagccga tcgcgctaaa ggtcagtttg atagcgaagg tctgatggat ttgagcaaac
    33721 tggaaggtgc gttcagcgat ccgacaatgc agttctatct ctgcggcccg gttggcttca
    33781 tgcagtttac cgcgaaacag ttagtggatc tgggcgtgaa gcaggaaaac attcattacg
    33841 aatgctttgg cccgcataag gtgctgtaat ttgatgttgc cggatggaaa catccggcaa
    33901 cccttgacgc ggtttaaatt gccgcgtcgt cctcttcacc ggtacggatg cgaatgaccc
    33961 gagccacgtc aaagacgaag attttaccgt caccgatttt gccggtttgc gccgtgcgaa
    34021 taatggtatc gacacaggta tcgacaatgt cgtccggtac gacaatctca attttcactt
    34081 tcggcagaaa atccaccata tactccgcgc cgcggtacag ctcggtatgg cctttctggc
    34141 gaccaaagcc tttcacttcg gtcaccgtca tgccggtaat accgacttcg gccagtgctt
    34201 cgcggacatc gtccagcttg aagggtttta taatcgcatc aatctttttc atgctattcc
    34261 ttgaaaaggt tgcctgtctt ttgatctgct aaacgtaaca cataacgcca attcattcct
    34321 tgaaatcgtt tgcatccagc tcgtgtcggg aaagcagttt ataaaattct gtccggttgc
    34381 gccccgccat tctcgccgcg tgggtgacgt tgcctttggt gatttgcagc agcttacgca
    34441 agtagttgag ttcaaaatga ttacgtgcct caacaaaggt tggcagcgcc gtattttcac
    34501 cctccagcgc ctgctccacc agcgcatcac taatcaccgg agatgaggtc agcgccacgc
    34561 actgttcaat cacgttgacc aactggcgca cattacccgg ccagctcgcg gtcatcaggc
    34621 gtttcatcgc atcggtagag aacgcgcgga caaacggttt atgtcgctct gccgcctggc
    34681 gcaacaggtg atttgccagt agcggaatgt cttctgtgcg ctccgccagt gccggaattt
    34741 tcaggctgac aacgttgagg cggtaataga gatcttcgcg gaattccccg cgcgccatcg
    34801 cttttgacag atcacggtga gtggcagaaa taatccgcac attgatatca atatcgcggt
    34861 tactgcccag cgggcgcact ttacgctcct gcaacacgcg cagcagtttg acctgtaacg
    34921 gcgcaggcat atcgccaatt tcatcgagaa atagcgttcc gccttccgcc gcctggaata
    34981 aaccttcgcg attgctgaca gcgccagtaa acgcgccacg cgcatgacca aacagctccg
    35041 actccagcaa ttgctcgggc aatgcgccac agttaatagc aataaatggt ttgctgttgc
    35101 gcgggctggc gttgtggata gcctgggcga aaatctcttt cccggtgccg ctctgaccgt
    35161 taatcaaaac gctgacgtct gattgcgcca ccagccgcgc ctgttccagc aaacgcagca
    35221 tcagcgggct gcgggtgaca attgcctcgc gccagcgttc atcggtggct ggcgcggatt
    35281 gctccagcgc atcgtcaatt gcctgatata gcgcatcttt atcgacaggc ttggtgagga
    35341 aactaaaaac gccctgctgt gttgcagcaa cggcatcggg gatagaacca tgcgcagtaa
    35401 gaataattac tggcattccc ggctgcactt tctggatttc agcaaatagc tgcataccgt
    35461 ccatttcatc catccgcagg tcgctgataa ctaaatctac tttttcgcga ttcagtaccc
    35521 gtaatccttc agcgccactt tccgccgtga ccacactgta gccttcgctg gtcaggcgca
    35581 ggccaagcag tttcagcaat cccggatcgt catcgaccaa taataaatgc gcaggtttat
    35641 ggctcatcag gagtgacctc atgggtggat ggcgcgggct tttcactctc gtgcggcgta
    35701 tccggcgaga aatttccagc cggtttgcgg gtcgagagct ggcattcaat atcggtcagg
    35761 ttttccagct tgcgggtggt gagttccagt tgctgctgta gaacgtgatg ttgctggcgc
    35821 aatgtatcca gctcgctgtc gctggactgc tggagtttac tgtaacgttg gcgctcttcc
    35881 gccagttgca gttgcagcgc ctgaccgtcg cgccagagtt gatacagtgg gcgaacctgt
    35941 gccgggatct cggtacttaa cgcttcaata cgcgcgacca gctggcggcg ctcatacggc
    36001 gtaattttgg cgtcggcgag caaaatccct tgtttaaagg tattttgcca gctgccgtcg
    36061 tcatattggc gagcttgctg acgcgactgc gcaggcatta aacgatcagc acaatccatc
    36121 gcccgcagcc agtaaagcgg attggtttcg gttgatttac cttgcagcgc ccagatgtcg
    36181 ctacattcag tagaaagata gtcagccagt tgataaaccg gaattttttc ttctgctggc
    36241 gtatcaatgg ctggcttatt gtgattctgc acgcaaccca gcaatgccag acatggcagc
    36301 cctgccagcc acagccgtcg gggcaataat cgttgaaaaa tgtgtcgcat attcaccaga
    36361 cttaaagcct atcccagtgg gcgtaattgt tgcagacagt ctggacatgg actgcgcgga
    36421 gaaaccgggg cgtacatatc gtacgtgaag atttcgagca ctacccgggg ccgaactgac
    36481 aaataaaata gcctgatagg ataggctgtt agcattattt cgtgtttttc gacgacggta
    36541 attcaatgcg gaaacaaacg tcttgcccgc tctcgtcgac cagatacagt tccccttgca
    36601 tacggcgaat acaatccctg gcaatgctta atcccagacc gctgcccttc accgcccctt
    36661 ttcgctggtg gcttccctga aaaaagggtt cgaagatcat ggcgcgttcc tcttgcggaa
    36721 tgggcgtgcc tgtattgatg acatcaatat aaacccgcgc accatgtaaa ctgctgcgaa
    36781 ggcaaatgtt accggattca gccccgtagt gcaccgcatt ggagtaaaga ttatccagta
    36841 cgctcatcag cagcattggc tccgccaggc aagctgttgc tttgagatcg acgtcggtat
    36901 gcatcatttt agcccgtgcg ggcaggctat gagcagaaac cactgtctcc accagcggtg
    36961 ctaattcaac attctccagt tccaccgcac tgtccgcctg tttacggttg taatcaagca
    37021 gttgttcgat cagtttttgc aaattgcagc tgctgctatc aagaatgctc accacctctt
    37081 tttgctctgg cgtaagcggc ccgacaacct gatcagccag taattcagtg ccctcgcgca
    37141 tactcgccag tggcgttttt aattcatgag ataaatgtct taaaaattga tggcgttggg
    37201 attccagcca tgacaggcgc tcacttaacc aaagaatacg ttgcccaacc gagcgtaact
    37261 cgctcggtcc acagaacgaa acgctattgc ccagagaacg cccttccccc agacggttga
    37321 tcatgcgctc gatatttttc accggcccga taatcatccg cgtgaaaagt agcaccatca
    37381 ccagactcac cagaaatagc accagcgatt gccaaccaaa atattgccca cgttcggcga
    37441 tttcacgctg aagttgctgc ccacgagaga acaccactgt gcgcgtggcc tgcaccattt
    37501 cggtattggc actggcaaag gcttcaagac gcgcggcggc ggcagcatcg ggaccactgt
    37561 tgttacactg aagttgggcc agattgttca aatcctgacg caatgcctgg taaagtttat
    37621 cgtctggcag tacgcctgcg tgggcatcga gcatttcgct gtaacgcttg cgctggcttt
    37681 gataaacctt cgcaagcgta gggtcgtcca gcacgcaata ctgacggtag ctacgctcca
    37741 tctccagcgc cgcgttggtc atcgcttcac tacgccgggc atcaataagc gtagtgcggt
    37801 taaccagcgc cgcctgatcg ctaagcgcat tcaggctttg ccaggcttgc catgccagca
    37861 ccaacagggg tagtagaatt agcaaaaatg ccagcattac cagttgtcgt aatgagcggg
    37921 gaaaaacggg ccagcgtttc aaggtgttac tctcgtcaga cgcgaatagc ctgatgctaa
    37981 ccgaggggaa gttcagatac aacaaagccg ggaattaccc ggctttgtta tggaataagg
    38041 cggtgcctaa ctcgacgttt cgcccgatgg ttgatatagc tacgctgata tcagaagttg
    38101 gacggcaggc accttgttgt gcgtcattcg aattttatgt agcacgtccc gaaggggctg
    38161 acataagtcg gtgaatgagc cactgcttac tattatgcag taactgtgcc aataaagaaa
    38221 atagtttggt aacgtactga ttatacgttg ctttgaggga tttatggctt cttcgctatt
    38281 tgaatgtaaa tcagccactg tgtcgctaaa aagagacaac ttaagataaa cttattagat
    38341 tgcaataaaa tcaatcattt aagtgtcgcc agaaagcgac acggtaaacc acccattgtc
    38401 gtgatttaca gacacaaaaa agctcccgga gttgggagct tatgatagtg gttggtgctt
    38461 aatgccgcat ccgggctgca aaaccaatga actgatgact taccccaact gcttacgcgc
    38521 attgcggaaa atgcgcatcc atgggccatc ctcgccccag ttttccggat gccaggagtt
    38581 gctgacagta cggaaaacac gttccgggtg cggcatcata atggtgactc gaccactttc
    38641 agtcgtaacc gccgtaatac cgttcggtga accgttcggg ttagccgggt aggtttcagt
    38701 gactttgccg aagttatcga catagcgcag tgccaccagc cctttgcttt ccagtgccgc
    38761 cagatgcgcc gcatcacgca cttccacgcg cccttcacca tgagagacag caatcggcat
    38821 ttgcgagccc accatcccct gcaacagcag agacgggctt tgggttactt caaccaggct
    38881 gaaacgcgct tcaaagcgat cggaggtgtt acgcacaaaa cgtggccaca actcactccc
    38941 cgggatcagt tcacgcaggt tagacatcat ctggcaaccg ttacataccc ccagggccag
    39001 cgtttgcgga cgatggaaga aggttgcaaa ctcatcgcgt acacggtcat tgaacaggat
    39061 agacttcgcc caaccttcac cggcacccag cacatcaccg taggagaaac ctccgcacgc
    39121 gaccagggcg tgaaaatcct ccagtcccgt acgtccagtc agcaggtcgc tcatatgcac
    39181 gtcgatagca tcaaagcccc cacgttggaa agctgccgcc atttcaacat gcgagttcac
    39241 gccctgctca cgcagtacgg caactttcgg acgtgcgcca gtggcaatat acggtgctgc
    39301 cacatcttcg ttgatatcga acgacagttt tacattcagg cccggatcgg cgtcgttaga
    39361 tttcgcctga tgctcctgat cggcacactc cgggttgtca cgcaggcgct gcatctgcca
    39421 ggtagtttct gcccaccaga cacgcaacgt agtgcggctt tcgctgaata cagtctgccc
    39481 gttggcggta atcacaaaac ggtcaccgga aaccgcctgc ccaacataat ggacgcaatc
    39541 cgcaagccca tgctgtgcca gtacggactc gaccgcttca cggtcagcgg cacgaacctg
    39601 aatcaccgca cccagttctt cgttaaacaa cgccgccagg cgatcgtcac ccagagaggc
    39661 gatatccgcg tcaatgccac aatgacctgc aaaggccatt tccgccagcg ttaccagcag
    39721 gcctccatca gagcggtcgt gatacgccag cagcttacgc tgtgcaacca gcgcctgaat
    39781 cgcgtcatag aagcctttca gttggacaac atcgcgtaca tctgccggtt tgtcgccaag
    39841 ctgacgataa acctgcgcca gcgccgttgc gcccagcgcg ttattgcctt tgcccaaatc
    39901 aatcagcagc agtgcgttat cttcggtaca aagctgcggc gtgatggtgt gacgtacatc
    39961 ttccacgcgg gcaaatgcag aaatcaccag cgacagcggc gacgtcattt cgcgctcttc
    40021 gttaccttcc tgccagcggg ttttcatcga catagagtct ttacccaccg ggatcgtcag
    40081 gcccagcgcc ggacaaagtt cttcacccac ggctttaacg gcttcataca ggcccgcatc
    40141 ttcaccaggg tggcctgccg ccgccatcca gttggcggaa agtttaatgc gtttgatatc
    40201 gccaatctgt gttgcggcaa tattggttaa cgcttcaccg accgccagac gggcagaggc
    40261 ggcgaaatcc agcagcgcaa ccggcgcacg ctcgccaatc gccatcgctt caccgtagta
    40321 gctgtcgagg ctggcggtag tgaccgcgca gttagcgacc ggcacctgcc acggccccac
    40381 catctgatcg cgcgccacca tgccggttac gctgcggtca ccgatggtca ccaggaaagt
    40441 tttctccgcc acggtcggca gatgcagtac gcgtttcacc gcgtctgcaa tggtgatccc
    40501 ttcacggacc agcgcatcgc ccttcgcttt cagcgtttgt acatcgcggg tcatcttcgg
    40561 cgttttacca agcaggacgt ccagcggcag atcgatcggc tgattatcaa aatgacgatc
    40621 gtgcagagaa agatgcagtt cttcggtcgc ttcaccaatc accgcgtagg gtgcgcgctc
    40681 acgcttacac agttcgtcaa acagcggtaa ttgatcggca gcaaccgcca gcacgtagcg
    40741 ttcctgggat tcgttacacc agatttccag cgggctcatg cccggttcgt cgcttagaat
    40801 atcgcgcagt tcaaatttac cgccgcgtcc gccgtcgctc accagctccg gcatcgcgtt
    40861 agaaagaccg cccgcaccga cgtcgtggat aaacagaatt gggttagcat caccaagctg
    40921 ccagcaacgg tcgatcactt cctggcaacg acgctccatc tccgggttgt cgcgctgtac
    40981 ggaagcaaag tcgaggtcgg catcagactg accagacgcc atggaagacg ccgcgccgcc
    41041 gccaagaccg atgttcattg ccgggccgcc gagaacgacc agcttcgcac cgacgttgat
    41101 ctcgcctttt tgtacgtgat cggcgcgaat gttgccgatc ccgcccgcca gcatgatcgg
    41161 tttgtgataa ccgcgcagct cttcgccatt gtggctgttc actttttctt cataagtacg
    41221 gaagtagccg ttcagtgccg gacgaccaaa ttcgttgtta aacgccgcac cgcccagcgg
    41281 gccttcggtc atgatgtcca gcgcggtaac aatgcgctca ggcttaccga aatcttcttc
    41341 ccacggctgt tcgaagccag gaattcgcag gttggatacg gagaaaccaa ccaaaccagc
    41401 tttcggcttc gcgccgcgcc cggtggcacc ttcatcgcgg atttcaccgc cggaaccggt
    41461 cgccgcgccc ggccacggag aaatcgccgt cgggtggttg tgagtttcga ctttcatcag
    41521 gatgtgcgcc ggttcctgat ggaaattgta gcggcccgtt tcgtggtcag caaagtagcg
    41581 gcccacttca gaaccttcca ttacggcggc gttatcttta taagcagaga gaacgtgatc
    41641 tggcgtggtt tcgaaagtat ttttgatcat cctgaacagc gatttcggct gttgttcacc
    41701 gtcgataatc cagtcggcgt taaaaatttt gtggcggcag tgctcagagt tcgcctgagc
    41761 gaacatatac agctcgatgt cgttcgggtt acgaccaagc tttgtgaaag catcctgcag
    41821 atagtcaatt tcatcttccg ccagagccaa gccaagacgc aggttagcgt cgatcagcgc
    41881 ctgacggccc tgccccagca aatcaacgct tgtgaccgga gtcggttgat ggtgggcaaa
    41941 caactgctct gcatcatcta aatcaaaaaa gaccgtttcc atcatgcggt cgtgcagttc
    42001 agcggtaacc tgctgccatt gttcattggt cagcgtaccg gcttctatat agtaagcaac
    42061 gccgcgctca aggcggttta cctgttgtag cccgcagtta tgggcaatat cggtcgcttt
    42121 cgaagaccag ggagagatgg tgccaggacg cggggtcacc agcaggagtt tgccttgcgg
    42181 ggcgtggctg gcgagtgccg gaccatattt cagcaggcgt tcaagttgtg cgtgctcatc
    42241 atcgtttaac ggcgcattga ggtcagcaaa atggacatac tcggcgtaaa tattgtgaac
    42301 cgggagcctg gcagcctgaa aacgtgccag cagtttgttg attcggaatg ccgacagtgc
    42361 aggcgaacca cgcagaattt ccatcataag tctctcgtct tctaagcttt cggtgtaccc
    42421 aagggggggg aaacgggcgt cattataaag aatctgatgc gctgacgaaa ccgtttgcgt
    42481 ggaaataaaa tcaccatcgt gaattagcaa cgcgtgccgc caatggctgt aataagttgc
    42541 catctggcgc aggtttacgc aaaatgccgc tcatttatga gtaaaccttt cactattatt
    42601 acgttttttc aagctgggac gcgcacgaca cagagaatta actaattgaa aaaattaaag
    42661 attaattatc tgttcatcgg cattctggca ctgctgctcg cggtcgctct ctggccatcc
    42721 attccctggt ttggtaaagc cgacaaccgt atcgccgcca ttcaagcgcg gggagagttg
    42781 cgtgtgagca ccattcatac tcccctgact tataacgaaa tcaacgggaa accttttggc
    42841 ctggattacg aactggcgaa acagtttgcc gattacctcg gcgtaaaact gaaagtgacc
    42901 gtgcggcaga atatcagcca gctgtttgac gaccttgata atggtaacgc cgacctgctg
    42961 gcggcaggac tggtctataa cagtgagcgg gtaaaaaatt atcagcctgg ccctacctat
    43021 tattccgtgt cacaacaact ggtttataaa gtgggtcagt atcgcccacg tacgctgggc
    43081 aacctgacgg cggagcaact caccgttgca ccgggtcatg tggtagttaa cgatctccag
    43141 accctgaaag aaacaaaatt cccggaatta agctggaagg tagacgacaa aaaaggctct
    43201 gcggaattaa tggaagatgt catcgaagga aaactcgatt acaccattgc tgattctgtc
    43261 gccatcagcc tgtttcagcg cgttcacccg gagctcgccg tagcgctcga tatcaccgat
    43321 gaacaaccgg tgacttggtt tagcccgtta gatggcgata ataccctttc cgccgccctg
    43381 ctcgacttct tcaacgaaat gaatgaagac ggtacgctgg cacgcattga agagaaatac
    43441 ctggggcatg gcgatgattt tgattacgtc gatacgcgca catttttacg cgccgtcgat
    43501 gcggtactgc cgcagttaaa gcccctgttt gagaaatacg ccgaagaaat tgactggcgt
    43561 ttgctggccg ctattgctta tcaggaatcg cactgggatg cacaggccac ttcaccgacg
    43621 ggtgtgcgcg gcatgatgat gttaaccaaa aataccgcgc aaagcctcgg cattacggat
    43681 cgtaccgatg ccgaacagag catcagcggt ggcgtgcgtt atttgcagga tatgatgagt
    43741 aaagtgccgg aaagtgtgcc ggagaacgaa cggatctggt ttgccctcgc tgcgtacaat
    43801 atgggctatg cgcatatgct ggatgcccgc gccctgacgg caaaaaccaa agggaatcct
    43861 gacagttggg ctgacgtaaa acagcgtctg cctttactta gccagaaacc ctattacagc
    43921 aagctgactt acggctacgc tcgtgggcat gaagcctacg cttatgtcga aaatattcgt
    43981 aagtatcaga ttagcctggt gggttatctg caagagaaag agaagcaggc tacagaagcg
    44041 gcgatgcaac tggcgcagga ttatccggcg gtatcgccta cggagttggg caaagagaaa
    44101 tttccttttc tctcgtttct ttcccagtcg tcatcaaact atttgaccca ttctccctct
    44161 ctgctgtttt ccaggaaagg gagtgaagag aaacaaaatt aatccgtcga ggattgcgct
    44221 tttttctgcg ctttaatttc ctggcggcgc atgcgaaaga agtcactgag caacgccgcg
    44281 cactcatccg ccagtattcc ttccgtaatt tccactcggt gattcatacc cggatgatgc
    44341 agcacatcca ttaaagatcc cgcagcgcca gttttcgcgt cacgcgcacc aaagaccacg
    44401 cgaccaatgc gactgtggat catcgctccg gcacacatta cacatggttc aagcgtgaca
    44461 tacaacgtgg cgtcgatcag acgataattt tgcatcacca gaccaccctg ccgcagggcc
    44521 atgatttctg catgtgcggt gggatcatgg cgaccaatcg ggcggttcca gccttcgccg
    44581 attacccgat tgttatgcac taataccgcg ccgaccggca cttcccgctc atcccaggca
    44641 cgtttcgcca gcgtcagcgc gtgacgcatc caatattcgt ggctaaattc gacttcagac
    44701 aaaaagaaaa ctccggttat aaaagcgcgg cgcattatac acggacgcta tgctttactc
    44761 tattccagtt gctggagttc accgcgcggg gttactcgcc aacgatgctg acagaaataa
    44821 agcagcggat tgtcctgatt actgtcgcta tagccactgt acagccgcag cggcgtgccg
    44881 attttgcgct ccagttgtgc gaccttttca tgtcccagac aacgcatcgt caatacccaa
    44941 ccaccatagc cacgctgaat ttggctggcg ataagattaa cccgcggcag ccagggcgta
    45001 tcgaaataaa ccgcttcaac cagcggctgc ggagagccgg taatcaacca gatatcagca
    45061 tcggaactta acaggtaggt ggttaatcgc tcctgaacca gcggaaaggc ggtaacattg
    45121 tcgcgaaacc agcgcacgaa atcggcctgc aacgtctgta aacgtgcttc gctgtgacca
    45181 aaagtgcacc cccacagaag cagactcatc ggccagcgtg ccgcacgacc ttttatcaat
    45241 aacgcaatgg ctataatcgg taacaacggc aggacaagta acgcattcag cggttggcga
    45301 cgcagtaaat agcgcagaaa actgccgaac atatcctgct gatgcaatgt tccatctaag
    45361 tcaaaaaaca ccacacgacg ctcgtgagtt gccaaaccat actcctctgg actgaaacat
    45421 cctgattaat tttcgctact acatagccta acagatagat catcactttt ccggcaacaa
    45481 tcgaccgcat catggctaac tggaattttt aattcatggc aattagcggc aatggaatat
    45541 aaaattcact cgcgtgtgtc tcatattgcc tgttgtcgcc actatttcat cgcgaggaaa
    45601 gatatgaacg gcttacttcg tatccgtcag cgttaccaag ggcttgccca gagtgataaa
    45661 aaactggcgg attatctgct gctacaacct gatacggcgc gccatttaag ctctcagcaa
    45721 ctggccaacg aagccggagt cagtcagtcc agcgtcgtga agttcgcgca aaaactcggc
    45781 tataaaggtt ttccggcgct taagttggcg ttgagtgaag cgctggcaag ccagccggaa
    45841 tcgccctccg tgcccattca taaccaaatc cgcggtgatg atccgttacg gctggtcggc
    45901 gaaaaactga ttaaagaaaa taccgccgct atgtacgcaa cgctaaacgt taatagtgaa
    45961 gagaaactgc atgaatgcgt aacaatgttg cgctctgcgc ggcggataat tctgaccggt
    46021 attggcgctt cgggtctggt ggcgcaaaac tttgcctgga agctgatgaa gattggcttc
    46081 aatgctgccg cactgcgcga tatgcatgcg ctactcgcaa cagtacaggc gtcgtcccct
    46141 gacgatctgt tattagccat ttcctacacc ggtgtacgac gcgagttaaa cctggcggca
    46201 gatgagatgc tgcgagtggg cggaaaagtg ctggcgatta ccggctttac tccgaatgcc
    46261 ctgcaacagc gtgcttctca ttgcctgtat accattgccg aagaacaagc gacaaacagt
    46321 gcttcaatct ctgcttgtca cgctcaggga atgttaacgg atttgctgtt cattgcgctg
    46381 attcagcagg atctggaact ggcaccagaa cgtattcgtc atagtgaagc gctggtgaaa
    46441 aaactggtct gagtaaagaa tgcgcgtata atgcccgccc ggtttgtgtt gttttgagag
    46501 tttccttatg gcgctgttaa tcactaaaaa atgcatcaat tgtgatatgt gtgaaatcaa
    46561 ataaaaacac atttattttc atagtgatac agcaatgtgt tattgaatgt gttgttggat
    46621 ttatggtgca aaaaaatttt gcataagttc agcaacacaa agaacaccac tttctttaac
    46681 gtcctccgcg ccctaaagga cggggcttta cggcgcaaaa ggcctgtgac atacaacatt
    46741 aacttttcag tcaaccgtag cacgttcttg tatacgccgt gtctgcggtt ttatataacc
    46801 ctggctggaa acctcttata caaagtcgat tcgccaacat catagatgat cgccaccttc
    46861 tggcgagaaa ctcctgatgc aattagtcgc ccggcctgcg cccattattc tggtgaaagt
    46921 ttgggacggc gaccgccaat tcgtccctgt gcgcgagcag cttccagtcc tgcttttgtt
    46981 cgttcaacaa tcagttcacg ttccatttca gccagggcac ccatcacatg gaagaaaaag
    47041 cgtcccattg gtgtgctggt atcaattgaa tcagtcagac tacgaaagtt gatgcctcgt
    47101 tcgcgcaacg cctccaccag cacgacaaga tgccgcatac tgcgccccag ccgatccagc
    47161 ttccagacaa ccagtgtgtc gcctgccgat aatgtcttga gcagtttttt cagccccggt
    47221 ctttcggact ttgtaccact tatcttgtct tcaaaaatca gctcgcatcc tgcacagttc
    47281 aacgcattac gttgt
//
LOCUS       DAZSNM010000008        46056 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZSNM010000008 DAZSNM010000000
VERSION     DAZSNM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46056
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.008"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            115..519
                     /locus_tag="ACHW42_000386"
     CDS             115..519
                     /locus_tag="ACHW42_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR8942357.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            545..1258
                     /gene="msyB"
                     /locus_tag="ACHW42_000387"
     CDS             545..1258
                     /gene="msyB"
                     /locus_tag="ACHW42_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR8942358.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            1407..1973
                     /gene="satP"
                     /locus_tag="ACHW42_000388"
     CDS             1407..1973
                     /gene="satP"
                     /locus_tag="ACHW42_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR8942359.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(2008..2595)
                     /gene="mog"
                     /locus_tag="ACHW42_000389"
     CDS             complement(2008..2595)
                     /gene="mog"
                     /locus_tag="ACHW42_000389"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR8942360.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2710..3663)
                     /gene="tal"
                     /locus_tag="ACHW42_000390"
     CDS             complement(2710..3663)
                     /gene="tal"
                     /locus_tag="ACHW42_000390"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR8942361.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3942..5372
                     /locus_tag="ACHW42_000391"
                     /pseudo
     CDS             3942..5372
                     /locus_tag="ACHW42_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            5442..6218
                     /gene="yaaA"
                     /locus_tag="ACHW42_000392"
     CDS             5442..6218
                     /gene="yaaA"
                     /locus_tag="ACHW42_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR8942362.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(6371..6667)
                     /locus_tag="ACHW42_000393"
     CDS             complement(6371..6667)
                     /locus_tag="ACHW42_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR8942363.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(6881..8167)
                     /gene="thrC"
                     /locus_tag="ACHW42_000394"
     CDS             complement(6881..8167)
                     /gene="thrC"
                     /locus_tag="ACHW42_000394"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR8942364.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(8168..9100)
                     /gene="thrB"
                     /locus_tag="ACHW42_000395"
     CDS             complement(8168..9100)
                     /gene="thrB"
                     /locus_tag="ACHW42_000395"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR8942365.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(9102..11564)
                     /gene="thrA"
                     /locus_tag="ACHW42_000396"
     CDS             complement(9102..11564)
                     /gene="thrA"
                     /locus_tag="ACHW42_000396"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR8942366.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(11590..11709)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(11645..11710)
                     /gene="thrL"
                     /locus_tag="ACHW42_000397"
     CDS             complement(11645..11710)
                     /gene="thrL"
                     /locus_tag="ACHW42_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR8942367.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(11924..12610)
                     /locus_tag="ACHW42_000398"
     CDS             complement(11924..12610)
                     /locus_tag="ACHW42_000398"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR8942368.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(13010..13150)
                     /gene="yjjY"
                     /locus_tag="ACHW42_000399"
     CDS             complement(13010..13150)
                     /gene="yjjY"
                     /locus_tag="ACHW42_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR8942369.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            13246..13962
                     /gene="arcA"
                     /locus_tag="ACHW42_000400"
     CDS             13246..13962
                     /gene="arcA"
                     /locus_tag="ACHW42_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR8942370.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(14021..15373)
                     /gene="creD"
                     /locus_tag="ACHW42_000401"
     CDS             complement(14021..15373)
                     /gene="creD"
                     /locus_tag="ACHW42_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR8942371.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(15431..16855)
                     /gene="creC"
                     /locus_tag="ACHW42_000402"
     CDS             complement(15431..16855)
                     /gene="creC"
                     /locus_tag="ACHW42_000402"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR8942372.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(16855..17544)
                     /gene="creB"
                     /locus_tag="ACHW42_000403"
     CDS             complement(16855..17544)
                     /gene="creB"
                     /locus_tag="ACHW42_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR8942373.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(17557..18030)
                     /gene="creA"
                     /locus_tag="ACHW42_000404"
     CDS             complement(17557..18030)
                     /gene="creA"
                     /locus_tag="ACHW42_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR8942374.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            18241..19110
                     /gene="robA"
                     /locus_tag="ACHW42_000405"
     CDS             18241..19110
                     /gene="robA"
                     /locus_tag="ACHW42_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR8942375.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(19107..19754)
                     /gene="gpmB"
                     /locus_tag="ACHW42_000406"
     CDS             complement(19107..19754)
                     /gene="gpmB"
                     /locus_tag="ACHW42_000406"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR8942376.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            19806..20326
                     /gene="yjjX"
                     /locus_tag="ACHW42_000407"
                     /pseudo
     CDS             19806..20326
                     /gene="yjjX"
                     /locus_tag="ACHW42_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(20411..20737)
                     /gene="trpR"
                     /locus_tag="ACHW42_000408"
     CDS             complement(20411..20737)
                     /gene="trpR"
                     /locus_tag="ACHW42_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR8942377.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(20827..22764)
                     /gene="sltY"
                     /locus_tag="ACHW42_000409"
     CDS             complement(20827..22764)
                     /gene="sltY"
                     /locus_tag="ACHW42_000409"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR8942378.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            22975..24642
                     /gene="ettA"
                     /locus_tag="ACHW42_000410"
     CDS             22975..24642
                     /gene="ettA"
                     /locus_tag="ACHW42_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR8942379.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEPPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(24749..24916)
                     /gene="nadS"
                     /locus_tag="ACHW42_000411"
     CDS             complement(24749..24916)
                     /gene="nadS"
                     /locus_tag="ACHW42_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFR8942380.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(24950..26182)
                     /gene="nadR"
                     /locus_tag="ACHW42_000412"
     CDS             complement(24950..26182)
                     /gene="nadR"
                     /locus_tag="ACHW42_000412"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR8942381.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(26203..27585)
                     /gene="radA"
                     /locus_tag="ACHW42_000413"
     CDS             complement(26203..27585)
                     /gene="radA"
                     /locus_tag="ACHW42_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR8942382.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(27634..28602)
                     /gene="serB"
                     /locus_tag="ACHW42_000414"
     CDS             complement(27634..28602)
                     /gene="serB"
                     /locus_tag="ACHW42_000414"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR8942383.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            28708..29352
                     /locus_tag="ACHW42_000415"
     CDS             28708..29352
                     /locus_tag="ACHW42_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR8942384.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            29380..30395
                     /gene="lplA"
                     /locus_tag="ACHW42_000416"
                     /pseudo
     CDS             29380..30395
                     /gene="lplA"
                     /locus_tag="ACHW42_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            30427..30690
                     /locus_tag="ACHW42_000417"
     CDS             30427..30690
                     /locus_tag="ACHW42_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8942385.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSIMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(30851..31570)
                     /gene="deoD"
                     /locus_tag="ACHW42_000418"
     CDS             complement(30851..31570)
                     /gene="deoD"
                     /locus_tag="ACHW42_000418"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR8942386.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(31627..32850)
                     /gene="deoB"
                     /locus_tag="ACHW42_000419"
     CDS             complement(31627..32850)
                     /gene="deoB"
                     /locus_tag="ACHW42_000419"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR8942387.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(32902..34224)
                     /gene="deoA"
                     /locus_tag="ACHW42_000420"
     CDS             complement(32902..34224)
                     /gene="deoA"
                     /locus_tag="ACHW42_000420"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR8942388.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(34351..35130)
                     /gene="deoC"
                     /locus_tag="ACHW42_000421"
     CDS             complement(34351..35130)
                     /gene="deoC"
                     /locus_tag="ACHW42_000421"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR8942389.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            35388..36929
                     /locus_tag="ACHW42_000422"
     CDS             35388..36929
                     /locus_tag="ACHW42_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR8942390.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            36901..37764
                     /locus_tag="ACHW42_000423"
     CDS             36901..37764
                     /locus_tag="ACHW42_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR8942391.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(37978..38760)
                     /locus_tag="ACHW42_000424"
     CDS             complement(37978..38760)
                     /locus_tag="ACHW42_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR8942392.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(38757..39830)
                     /locus_tag="ACHW42_000425"
     CDS             complement(38757..39830)
                     /locus_tag="ACHW42_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR8942393.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(39952..40113)
                     /locus_tag="ACHW42_000426"
     CDS             complement(39952..40113)
                     /locus_tag="ACHW42_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR8942394.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(40230..40835)
                     /gene="osmY"
                     /locus_tag="ACHW42_000427"
     CDS             complement(40230..40835)
                     /gene="osmY"
                     /locus_tag="ACHW42_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR8942395.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(41228..42817)
                     /gene="prfC"
                     /locus_tag="ACHW42_000428"
     CDS             complement(41228..42817)
                     /gene="prfC"
                     /locus_tag="ACHW42_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR8942396.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(42908..43585)
                     /gene="yjjG"
                     /locus_tag="ACHW42_000429"
     CDS             complement(42908..43585)
                     /gene="yjjG"
                     /locus_tag="ACHW42_000429"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR8942397.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(43600..44046)
                     /gene="rimI"
                     /locus_tag="ACHW42_000430"
     CDS             complement(43600..44046)
                     /gene="rimI"
                     /locus_tag="ACHW42_000430"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR8942398.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(44015..44428)
                     /gene="holD"
                     /locus_tag="ACHW42_000431"
     CDS             complement(44015..44428)
                     /gene="holD"
                     /locus_tag="ACHW42_000431"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR8942399.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            44531..45562
                     /gene="rsmC"
                     /locus_tag="ACHW42_000432"
     CDS             44531..45562
                     /gene="rsmC"
                     /locus_tag="ACHW42_000432"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR8942400.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            45839..45925
                     /locus_tag="ACHW42_000433"
     tRNA            45839..45925
                     /locus_tag="ACHW42_000433"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45873..45875,aa:Leu,seq:cag)
     gene            45954..46040
                     /locus_tag="ACHW42_000434"
     tRNA            45954..46040
                     /locus_tag="ACHW42_000434"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45988..45990,aa:Leu,seq:cag)
BASE COUNT        10868 a        12537 c        11651 g        11000 t
ORIGIN      
        1 cgttcatcaa aaaattgtaa taaaaagaaa agattacgtg cctgaatctt ctctttatca
       61 gcagtaaact agtgggtatt catcccccta cctcttccca ctaagagaat ccttatgaaa
      121 tccgttttta cgatttccgc cagcctggcg attagcctga tgctgtgctg cacggcgcag
      181 gcaaacgacc ataaactcct cggcgtcatt gcaatgccgc gtaacgaaac caacgatctg
      241 gcgctgaaac ttcctgtttg tcgcattgtg aaacgcatac aactctctgc cgaccatggc
      301 gatttacagt taagtggtgc atcggtttat ttcaaagccg cccgtagcgc cagtcagagc
      361 ctgaatattc cttcagaaat aaaagaaggg caaaccactg actggatcaa cattaacagc
      421 gataacgaca ataaacgctg cgtctcaaaa atcacctttt ccggtcatac ggtgaactca
      481 tcggatatgg ccacgctgaa aattatcggc gacgactaac gccagaatat agaagccaca
      541 aaaaatgaat gttaattacc tgaatgattc agatctggat tttcttcagc attgtagtga
      601 ggaacagttg gcaaatttcg cccgattgct cacccataat gaaaaaggca aaactcgcct
      661 ctccagcata ctgatgcgca acgaactgtt taaatcgatg gaagggcatc ccgagcaaca
      721 tcgccgcaac tggcagctga ttgccggaga attacagcat tttggtggcg atagtatcgc
      781 caacaaactg cgcggacacg gtaaattgta tcgggccatt ttgctcgatg tttcaaagcg
      841 attgaagctg aaagccgaca aagagatgtc tacgtttgaa attgagcaac agttactgga
      901 acaatttctg cgtaatacct ggaagaaaat ggacgaggaa cataagcagg agtttctgca
      961 cgcggtcgat gccagggtga atgagctgga agagctgctg ccgctgctga tgaaagacaa
     1021 attattggca aaaggtgtgt cgcatttgct ttccagccaa ctgacccgca ttttacgcac
     1081 ccacgcagca atgagcgtac ttgggcatgg tttgctgcgc ggcgcggggc tgggaggccc
     1141 tgtgggcgca gcattaaatg gggttaaagc ggtcagcggc agcacctatc gcgtgacgat
     1201 tccagccgta ctgcaaatcg cctgcctgcg ccggatggtt agcgccactc aggtctgata
     1261 ccggaaaccg gggaaaattt tcattttccc ctcaaaagat cgtagacact gccccactgg
     1321 ctgattatta tgccgcgccc tgaaaacact acagttattc agggaaatta tttcaccatt
     1381 cattcgatga tgatttttga ggaattatgg gcaacactaa gttggctaat ccggcaccgc
     1441 tgggcctgat gggcttcggc atgaccacca ttctgcttaa cctgcacaac gtgggttatt
     1501 tcgctctgga cggtattatt cttgccatgg gcattttcta cggcggcatc gcgcaaattt
     1561 ttgctggtct gctggagtac aaaaaaggca acactttcgg tttaaccgca ttcacctctt
     1621 acggttcttt ctggctgacg ctggttgcga ttctgctgat gccgaaactg ggtctgaccg
     1681 atgcgccaaa tgcacagttc cttggtgtct acctgggtct gtggggcgta tttacgctgt
     1741 ttatgttctt cggcactctg aaaggcgcgc gcgtcctgca attcgttttc tttagcctga
     1801 ccgtgctgtt tgccctgctg gcgatcggta acattgccgg taatgccgcg atcatccact
     1861 ttgccggctg gattggtctg atctgcggtg ccagcgcaat ctatctggcg atgggtgaag
     1921 tgctgaacga gcagtttggt cgcaccgttc tgccgattgg tgaatcccac taattgtctt
     1981 gagatccccc cgctcggggg gattttttta ttcgctaacg tcgcgtcttg cactcttcgg
     2041 tctgaatgca gcaaccactt ctggtgccgt ttcaacgtat ggcccttcca gcaactgaat
     2101 gcagtacggt acgctggcaa aaataccatg caccacaacg ttaccctcag cgtccttcac
     2161 accttccagc gtctctttaa tagacttcgg ctgaccgggt aagttaagga tcagcgcctg
     2221 tttgcgaatc acgcccacct gacgcgaaag gatcgcagtt ggtacaaaat gcaggctgat
     2281 ctggcgcatc tgttcaccaa agccaggcat ctcgcggtcc gctactgcca gcgtcgcatc
     2341 gggcgttacg tcacgacgcg ccgggccagt tccgcccgtg gtgagcacca gatggcaact
     2401 catttcatcc accagctcac acaacgtttg ctcgatgatc gcctgctcat cggggattaa
     2461 gcgggtttcc agttcaaacg gcgtggttag cgccgatgtc agccattctt ccagcgctgg
     2521 aatgccttta tcctgataaa cgccgctgga tgcgcgatca gagatggaaa ctaagccaat
     2581 acgtaaagta ttcatcaaga tttttccgtt taaacaatct cgttaaacag aatgatacac
     2641 tgcgaaggga gtgacagaca ggcttcagaa gaggtagcgt gaccgacttc ccggtcacgc
     2701 taagaatgat tacagcagat cgccgatcat tttttccagt ttttcctggt caatagcaaa
     2761 cttacggata ccttccgcca gtttatctac tgccattgga tcctggttgt gctgccacag
     2821 gaactcggac tcagtgatac gcgccggacg cgctttcact tcgccggtgt aagacagttt
     2881 acgttcgata gccccttcgc tctccgccag ctctttcagc agtgccggtg cgatggtcag
     2941 acggtcacag cctgccagtt ccagaatttc gccgatgtta cggaagcttg cgcccataac
     3001 cacggtttca taaccgtgct ctttgtagta ctggtagatt tcagatacag aaaccacgcc
     3061 cggatcttct gccggagcgt actctttctt atcggtattc gctttgtacc agtcaagaat
     3121 acggccaaca aacggcgaga tcaggaacac gcccgcctcc gcacaagcac gagcctgagc
     3181 gaaggagaac agcagggtca ggttacagtt gataccttct ttttccagct gttctgcagc
     3241 acggataccc tgccaggtag aagccagttt gatcagaata cgatcgttgc taatacctgc
     3301 atcgttgtag agtttgatca ggcgttttgc tttcgcaatt gacgcttcag tgtcatagga
     3361 aagacgcgca tcaacttcag ttgagatacg gcccggaacc agtttcagga tttccagacc
     3421 aatatttact gccagtttgt cggtcgcgtc cacgatctgc tgcgcgcgat cgttgctctg
     3481 ctgtttcgcc caggcgacag catcatcaat caacttacgg tattccggaa tctgcgctgc
     3541 gttaagaatg agagaagggt tggttgtggc atcctgcggt tgatacagct tcattgccgc
     3601 gatgtcccca gtgtcggcca ctacggtggt gtactgacga agggaggtca atttgtccgt
     3661 catgatagta tttctcttta aacagcttgt tagggggatg taaccggtct gccctgatga
     3721 tatcacgacg caacgtcagc gcaaccgccg acaagacggt tatcgccagg cgagactgtt
     3781 tcggatttct gacactgcgt tgatatcgcc caccattttt atacaaaacc tcatgtatgc
     3841 tacgcagaag ttatcaagta cctcgtagag tatatacttc ttaaacaatt ggtaacgttt
     3901 acacaggaaa gtcatcgcga ccggcaataa gagggatatg catgccagat tttttctcct
     3961 ttattaacag cgtcctttgg ggatcggtaa tgatttacct gctcttcggc gcaggttgtt
     4021 ggttcacttt tcgcaccgga tttgtgcagt ttcgctacat ccgccagttt ggcaaaagtc
     4081 ttaaaaatag cattcatcca cagccaggcg gtttaacctc atttcagtca ttgtgtacca
     4141 gtcttgcggc gcgcgtgggt agcggcaacc tggccggcgt tgcgctggca attaccgccg
     4201 gtggaccagg tgccgtcttt tggatgtggg ttgctgcgtt tatcggcatg gcgacctcgt
     4261 ttgccgaatg ttcccttgca caactttata aagaacgtga cgtcaatggg cagtttcgtg
     4321 gcggaccggc atggtatatg gcgcgcgggc tggggatgcg ctggatgggc gttctgttcg
     4381 ccgtcttttt gctcatcgcc tacggcataa ttttcagcgg agttcaggcg aacgccgttg
     4441 cccgagccct gagtttttct tttgattttc ctccgctggt gacgggcatt attctcgctg
     4501 tctttgctct gctggcaatc actcgtggtc ttcatggcgt cgcccggctc atgcaggggt
     4561 ttgtcccgtt gatggcgata atctgggtac tgaccagcct ggtgatttgc gtaataaata
     4621 tcgggcaact tccccacgtc atttggtcta tttttgagag tgcttttggc tggcaggaag
     4681 cggcaggcgg cgcggcggga tataccttaa gccaggcgat tactaacggt tttcagcgca
     4741 gtatgttttc caatgaggtg ggaatgggat cgacgccaaa cgcggcagcg gcagctgcat
     4801 cctggcctcc gcatccggca gcacaaggga ttgtccagat gattggcatt tttatcgaca
     4861 ccctggtcat ctgtacggca agcgccatgc tgatattact ggcgggtaac ggcacaacct
     4921 acatgccgct ggaaggtatt cagcttatcc agaaggcgat gcgggtgcta atgggttcct
     4981 agggtgctga atttgttacc ctcgtggtta ttctgtttgc cttcagctcc atcgttgcca
     5041 actacattta tgccgaaaac aatctcttct ttttacgcct gaacaaccct aaagcgatct
     5101 ggtgtttgcg gatctgcacc ttcgcaacgg tcatcggcgg caccttgcta agccttccgc
     5161 tgatgtggca actggcagat atcataatgg cctgcatggc tattaccaat ttgaccgcca
     5221 ttttactgct ctcgcctgtg gttcatacca ttgccagtga ttatctacgc cagcgtaaac
     5281 tcggcgtgcg cccggtgttt gatccgttgc gttatccgga tatcgggcgc cagctttctc
     5341 cggacgcgtg ggatgatgtt tcgcaggagt aatcacaact atcgatcaac tcattctctt
     5401 tttttgctaa agtcggcata aatttcctgc aaggactgga tatgctgatt cttatttcac
     5461 ctgcgaaaac gcttgattac caaagcccgt tgaccaccac gcgctatacg ctgccggagc
     5521 tgttagacaa ttcccagcag ttgatccatg aagcgcggaa actgacgcct ccgcagatta
     5581 gcacgctgat gcgcatcagc gacaaactgg cgggtatcaa cgccgctcgc tttcatgact
     5641 ggcagccaga tttcacgccg gcgaatgccc gccaggcgat tctggcgttt aaaggtgatg
     5701 tctacaccgg cttgcaggcc gaaaccttca gcgaagacga tttcgatttt gcccaacagc
     5761 atttgcgaat gctttccggc ttgtatggcg tactgcgccc gctcgattta atgcagcctt
     5821 atcgtctgga aatggggatc cgtcttgaga atgcccgagg gaaagatctg tatcaattct
     5881 ggggagatat catcaccaac aagctgaacg aggcgctcgc agcacaaggc gataatgtgg
     5941 tgattaatct ggcgtcagat gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg
     6001 agattatcaa gccggtgttc ctcgatgaga agaacggcaa atttaagatc atcagcttct
     6061 acgctaagaa agcacgcggt ctgatgagtc gtttcattat tgaaaatcgg ctgaccaaac
     6121 cagagcaact gactggtttt aatagcgaag gttacttctt tgatgaagat tcctccagca
     6181 atggcgaact ggtgtttaaa cgctacgagc agcgttaagt ttgtagtcaa tgccggatgc
     6241 ggcgtgaacg ccttatccgg cctacaaaag catgcagatt caatatattg cagaattaac
     6301 gtaggcctga taagacgcgc cagcgtcgca tcaggcgctg aatgccggat tgttacccgg
     6361 catttgtcat ttagcggtga tgtttgcctg gaccatgacc gccgtgatga tcatgaggag
     6421 ctttcttatg gtggcgcggc ggtggcggcg gtccgtgtgg gtgccagcga ttgcctcgcc
     6481 attcataatg ttgtttccac cagccgtggt cgcgccagtg acctccatcc cagtaatagc
     6541 cacgattatc acgatcgcct atctgtaatt ttactgacgg gactaacgta atttccgcag
     6601 cctgtgctgc catgggagcg accagaacca gggaaagtgc gagtacgata gattgcatct
     6661 ttttcacagg ttactccttt tatcattgcc cgttatgggt cgatatccgg agattacgcg
     6721 atgagaacga cggttgttat tctccgcaat cctaatctct aagttaccgc atttattgat
     6781 aatttctcct ttttccctgt cgtttttctc cataatttct tcataaaaaa gccgggctgc
     6841 ataaaagcaa acccggcctg attgagataa tgaatagatt ttactgatga ttcatcatca
     6901 atttacgcaa cgcagaaaaa tcggcgggca gattatgtga aagcaagggt aaatcagcac
     6961 gttctgccag ctcttttggc agatccaacg tttcaccgag aatcgcttcc acgctctctt
     7021 taaatttcgc cggatgcgcg gtgccgagga acaagccata ttcgcctgga ttcaactggt
     7081 cacgcagcgc acgatacgct acggcagcgt gcggctccga ggtgtagccc agttctttta
     7141 actcacgcat tgtctgttgc gtggtttcat catccacggc tgcataaccc agctctttca
     7201 gttgccagat tttgcggcgg aacaactctt ccacacgcgg ccagttgttc ggctgactca
     7261 cgtccatcgc gttggataac gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca
     7321 ggaaacgtgg cacggtatcg ttcacgttgg tcgcagcaat aaaacgtttc accggcagac
     7381 cgaatgactt cgccagcaga cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga
     7441 caaccagctg gttgcgcgcc tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa
     7501 tctgcgccag caaacggctg atgttaatcg agttagccga gtttaacccc agcgccactt
     7561 tcagttcttc atcatcaaac gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt
     7621 cgatggcaac agtttcgata ttgccgccca acgtacagaa cagtttttct tgcagtggac
     7681 tgattttgcc tcgtggatag aggataacca ctttcacatt cggcaaaccg tagaaagcat
     7741 gagccactgc agctccggta tcaccggagg tcgcggtcag aatggtcact ggcttatcat
     7801 ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc gaaatcttta aatgccagcg
     7861 ttggcccgtg gaacaattcc agacaaccga catcgctttc aacattggcg accggagccg
     7921 ggaaggcaaa cgccgcgcgc acgcgctctt ccaggatttc ctgtgggatt tcatcaccaa
     7981 taaacgccga gaggatcttc gcactgcggg tgacaaaatc cagcttcagc atctcatcaa
     8041 tttcagtcag gctgaattcc ggcaggtcgt gcggaaaaaa cagcccctga tttttgccca
     8101 acccctgggt tacggcttgc gcaaagctga cctgctcgtt gtgatctttc agattgtaga
     8161 gtttcattta gttttccagt actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa
     8221 aaccttcctg attttgcagg tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat
     8281 ccggcttgtc acatagagcg aacaaggtcg ggccggagcc ggagataccg ctcgctaccg
     8341 cgccgatttc cgcgaccgcc tgccgcgcct gccggaagcc aggcagtaac cgttcacggt
     8401 agggttcagc gataacatct ttcatcagct tcgcggcaag ctcaggctga cgggaatagc
     8461 aggcgtgaat gaagccagcc agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact
     8521 gcgccggtaa aatagcccgg gcttctgccg tcgagacttt aattcccgga tacgccagca
     8581 cccacagcca ctcatcaaac cctggcactt gctggctgat gatgtcgttt tcttcgatca
     8641 tcaactgcat accaccaaga aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga
     8701 tacgtccttc cagctcgccc atcaaagcca gcaaacgagt gtcattaagc ggcttgccgc
     8761 agtgttcatt catcgccatc agcgccgcga ccaccgaaca ggcgctggag cctaagcccg
     8821 aaccgatcgg catattcttt tccagagtca tcgccactgg aatttgcttg ccaagctcct
     8881 ggcaaaaacg ctcccagcac tgataaacga tattttcccg tggttctgac ggcagcttat
     8941 cggcaaagcg tccgagtttg ttgagactga atgtctctgc cgcctcaacc gtgactacat
     9001 ctccgagcaa tgcaccatca acgggtgcca ccgccgcccc gagcacatca aacccgacgc
     9061 tcatattggc actggaagcc ggggcataaa ctttaaccat gtcagactcc taacttccat
     9121 gagagggtac gtagcagatc ggcaaagaca ccggcagcgg taacgtcatt gcccgcacca
     9181 tatccgcgca gcaccaacgg cagcggctga taatagtggc tataaaaggc cagggcgttt
     9241 tcgccatttt tcactttgaa cagcggatca ttcccatcca cttcggcaat cttcacgcgg
     9301 cagacaccat cttcatcaat attgccaaca tagcgcaaaa cttttccttc atcacgggcc
     9361 ttcgccacgc gcgcggcaaa gagatcgtcg agctgtgaca gattcgccat aaaagcggca
     9421 acatcaccct cggcgttaaa ctctgcgggc agcacaggtt caatttcaat atccgccagc
     9481 tccagttcac gtcccgtttc acgagcgaga atcaatagtt tacgcgccac atccatacca
     9541 gaaagatcat ctcgcgggtc cggttcggta taacccattt cccgcgccag cgtggtcgcc
     9601 tcggagaaac tcatgccttc gtctaacttg ccgaagatat aagaaagcga acctgaaaga
     9661 atgccggaga acttcaccaa ttcatcacct gcattgagca gattttgcag gttctcaata
     9721 accggtaatc cagccccaac gttggtgtca tagaggaatt tacgccgcga tttttccgcc
     9781 gcataacgca actgatgata gtaatccatc gacgaggtgt tggccttttt gttcggcgtg
     9841 acaacgtgga aaccttcgcg caggaagtcg gcatattgat ccgccactgc ctggctggag
     9901 gtgcagtcaa caatgaccgg gttcagcaga tgatattctt tcacgaggcg aattaagcgc
     9961 ccgagattaa acggctcttt ggcttgtgca agttcttcct gccagttttc cagattaagg
    10021 ccatgcacac tagtgagcag agccttcgag ttggcaacgc cgcagacacg taagtcgata
    10081 tgtttattct tcagccagct ttgctgacgc ttcagttgct ccagcagcgc accgccaacg
    10141 ccaccgacgc caatcacaaa cacttcgata acctgatcgg tattgaacag catctgatga
    10201 gtaacgcgca cgccagtggt cgcatcatcg ttatttacca cgacagagat tgagcgttca
    10261 gaagatccct gagcaatggc gacaatgttg atattggcgc gggccagcgc ggcaaagaat
    10321 ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta ccaccgagat aatggccagc
    10381 cgttccgtca ccgccagcgg ctccagtaag ccttctttca gttccaggta gaactcttcc
    10441 tgcattgccc gttcagctcg cacacagtcg ctttgtggaa cgcagaaact gatactgtat
    10501 tcggaagatg attgcgtaat cagcaccacg gaaatacggg cgcgtgacat tgcagcaaag
    10561 acgcgcgccg ccatgccaac catccctttc atccccgggc cggaaacgct gaacattgcc
    10621 atgttattca gattggaaat gcccttgacc ggtaattcgt cttcatcacg gctggcacca
    10681 atgagcgtac ctggtgcttg aggatttccg gtatttttaa tcaggcaagg gatctggaac
    10741 tgggcgatgg gggtaatggt gcgggggtga agaactttag cgccgaagta ggaaagctcc
    10801 atcgcttcct ggtaggacat cgacttcaac aacctcgcat cgggcacctg acgcgggtcg
    10861 caggtataga ccccgtcaac gtccgtccaa atctcgcaac aatcggcgcg taaacaggca
    10921 gccagcaccg ccgcggagta gtcggaaccg ttacgtccaa gcaccaccag ttcgcctttt
    10981 tcattaccgg cggtgaaacc tgccatcaac accatgtgat cagccggaat gcggcttgcc
    11041 gcaatacggc gggtggactc agcaatatcg acggtagatt cgaggtaatg ccccactgcc
    11101 agcagttttt cgaccggatc gataacggta acgttgtgac cacgcgcttc taacacgccg
    11161 gccataatgg cgatcgacat tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc
    11221 gggcactgcc ccaacaaact aatgccatgc aggacatgtt ttatttgggc aaattcctga
    11281 tcgacgaaag ttttcaattg cgccagcggg aaccccggct gggcggcggc gagtcccgtc
    11341 aaaagttcgg caaaaatacg ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta
    11401 atggtttttt caatcatcgc caccaggtgg ttggtgattt tggcgggggc agagaggacg
    11461 gtggccacct gcccctgcct ggcattgctt tccagaatat cggcaacacg cagaaaacgt
    11521 tctgcatttg ccactgatgt accgccgaac ttcaacactc gcatggttgt tacctcgtta
    11581 cctttggtcg aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta
    11641 cgcgtcagcc cgcaccgtta cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa
    11701 tgcgtttcat ggatgttgtg tactctgtaa tttttatctg tttgtgcgct atgcctatat
    11761 tggttaaagt atttagtgac ctaagtcaat aaaattttaa tttactcacg gcaggtaacc
    11821 agttcagaag ctgctatcag acactctttt tttaatccac acagagacat attgcccgtt
    11881 gcagtcagaa tgaaaagctg aaaaatactt actaaggcgt tttttatttg gtgatatttt
    11941 tttcaatatc atgcagcaaa cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca
    12001 ggcgttgctg taaccagtcg accagtttta tgtcatctgc cactgccaga gtcgtcagca
    12061 atgccatgac tcgttcgcgt aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt
    12121 tcgccggttg ttgtattaat gttgctaatt gatagcaata gaccatcacc gcctgcccca
    12181 gattgagcga aggataatcc gccaccatcg gcacaccagt aagcacgtca gccaacgcta
    12241 actcttcgtt agtcagcccg gaatcttcgc gaccaaacac cagcgcggca tggctcatcc
    12301 atgaagattt ttcctctaac agcggcacca gttctactgg cgtggcgtag taatgatatt
    12361 tcgcccgact acgcgcagtg gtggcgacag tgaaatcgac atcgtgtaac gattcagcca
    12421 gtgtcgggaa aactttaata ttatcaataa tatcaccaga tccatgtgcg acccagcggg
    12481 tggctggctc caggtgtgcc tgactatcga caatccgcag ttcgctaaaa cccatcgttt
    12541 tcattgcccg cgccgctgcc ccaatatttt ctgctctggc gggtgcgacc aggataatcg
    12601 ttatacgcat attgccactc ttcttgacca aataaccgcg aacagggtga tcactgtcaa
    12661 cttattacgc ggtgcgaatt tacaaattct taacgtaagc cgcagaaaaa gccctttact
    12721 tagcttaaaa aaggctaaac tattttctga ctgcactaac ggtttaattg ttaaaaaatg
    12781 ctacatatcc ttctgtttac ttaggataat tttataaaaa ataaatctcg acaattggat
    12841 tcaccacgtt tattagttgt atgatgcaac tagttggatt attaaaataa tgtgacgaaa
    12901 gctagcattt agatacgatg atttcatcaa actgttaacg tgctacaatt gaacttgata
    12961 tatgtcaacg aagcgtagtt ttattgggtg tccggcccct cttagcctgt tatgttgctg
    13021 ttaaaatggt taggatgaca gccgtttttg acactgtcgg gtcctgaggg aaagtaccca
    13081 cgaccaagct aatgatgttg ttgacgttga tggaaagtgc atcaagaacg caattacgta
    13141 ctttagtcat gttacgccga tcatgttaat ttgcagcatg catcaggcag gtcagggact
    13201 tttgtacttc ctgtttcgat ttagttggca atttaggtag caaacatgca gaccccgcac
    13261 attcttatcg ttgaagacga gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg
    13321 gaaggctatg atgttttcga agcgacagat ggcgcggaaa tgcatcagat cctctctgaa
    13381 tatgacatca acctggtgat catggatatc aatctgccgg gtaagaacgg tcttctgtta
    13441 gcgcgtgaac tgcgcgagca ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac
    13501 gaagtcgata aaattctcgg cctcgaaatc ggtgcagatg actacatcac caaaccgttc
    13561 aacccgcgtg aactgacgat tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt
    13621 actgtcagcg aagaacgtcg tagcgttgaa agctacaagt tcaatggttg ggaactggac
    13681 atcaacagcc gttcgttgat cggccctgat ggcgagcagt acaagctgcc gcgcagcgag
    13741 ttccgcgcca tgcttcactt ctgtgaaaac ccaggcaaaa ttcagtctcg tgctgaactg
    13801 ctgaagaaaa tgaccggccg tgagctgaaa ccgcacgacc gtactgtaga cgtgacgatc
    13861 cgccgtattc gtaaacattt cgaatctacg ccggatacgc cggaaatcat cgccaccatt
    13921 cacggtgaag gttatcgctt ctgcggtgat ctggaagatt aatcggcttt accaccgtca
    13981 aaaaaacggc gctttttagc gccgttttta tttttcaacc ttatttccag atacgtaatt
    14041 catcgtccgt tgtaacttct ttactggctt tcattttcgg cagtgaaaac gcataccagt
    14101 ctatattacg agtcacaaac atcatgccgg ccagcgccac caccagcaca ctggttccca
    14161 acaacagcgc gctatcggca gagttgagca gtccccacat cactccatcc agcaacaaca
    14221 gcgcgagggt aaacaacatg ctattgcgcc agcctttcaa taccgcctgc aaataaatac
    14281 cgttcattaa cgccccaatc agactggcga ttatccatgc cacggtaaaa ccgatatgtt
    14341 cagaaagcgc cagcaagagc aaataaaaca tcaccaatga aagccccacc agcaaatatt
    14401 gcattgggtg taaacgttgc gcggtgagcg tttcaaaaac aaagaacgcc ataaaagtca
    14461 gtgcaatcag cagaatggcg tacttagtcg cccggtccgt taattggtat tgatcggctg
    14521 gcgtcgtcac tgcgacgcta aacgcgggga agttttccca gccggtatca ttgcctgaag
    14581 caaaacgctc accgagatta ttagcaaacc agctgctttg ccattgcgcc tgaaaacctg
    14641 actcgctagc ttcccgtttg gctggtagaa aatcacctaa aaaactggga tgcggccagt
    14701 tgctggttaa ggtcatttcg ctattacgcc cggcaggcac cacagaaaga tcgccggtac
    14761 cgcttaaatt cagtgccata ttcagctgca ggttctgctt ccgccagtcc ccttcgggta
    14821 aagggatatg cacgccctgc ccgccttgct ctaacccggt accgggttca atggtcagtg
    14881 ccgttccgtt aacttcaggc gctttcacca caccaatacc acgcgcatcc ccgacgctaa
    14941 tcacaataaa gggcttgcct aagatgatat ttggcgcgtt gagctcgcta agacgcgaaa
    15001 catcgaaatc ggcttttagc gttaaatcac tgtgccagac ctgaccggta taaatcccta
    15061 tcttgcgttc ttccacgttc tgattgccat caaccatcaa tgactcaggt aaccaaaaat
    15121 ggataaaact tcgtttccgc tccacggttt tatcctcttc ctgcaccgta taaagctcgg
    15181 tcacaggaat agcgataagc ggcccaacga gtttttgcgg cccgctggta ctttggcgaa
    15241 tcgcatcttc cacatcgcta cggtaatcgg cacgttcgac aatcacttgc cgaatcagca
    15301 ttatcggaat caacaacagc aatactgcac caaacaaggt ggtcattttc cagaacaggg
    15361 gggatttcaa catagtcttc tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa
    15421 gaatttgaag ctatgtgaag tgacggtgaa gtcgtagcga ggccagcacg ccaccttcct
    15481 gcacgttgcg cagcgtgact tcgccgttaa acaaacgggc gacctcactg acgaacgcca
    15541 accccagacc gctgcttttt tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa
    15601 tacgtgacag cgcgtagtca ggaataccac taccggtatc cagcacctta agcgtgacgt
    15661 gttcctgatc cacttcggcg cttagcgtta tgcaaccgct ctcgggagta aaatcgatgg
    15721 cgttatccag taaattcccc agcgcctgat ccagtaacgc cggttcagca gcaacattaa
    15781 cctcggtggg cataacatgc aaagtgatgt ttttttctgc caactgcacg gtgcgcgctt
    15841 cgctgacgcg gcgaaataat gccgccacat caacagcagt cagaacgact tcctgacgat
    15901 tctccagtct tgcctggcgt agtaacgttt ccaccagcgc ctgcattcgc gcattttgtg
    15961 tcagaatgtt gtcagtaaaa cgagccacta cttcaggcgg cggaccttcg cgtaaaattt
    16021 ccgccgcgcc acgaatcgcc gccagtgggc tttttagctc atgggttaac gcataaacat
    16081 actgctcaat atagtttttc ccttccagct tcacgcgcat actttccagc gcctgcgcga
    16141 gtttacgcag ctcgctacta ccgagatcgg gaagaggaac gggcttattg tcagtgacgg
    16201 aatcagcata gcgagtgagc cgggcaatgg agcggttgat ccaccaaacc atgcctgcgc
    16261 caatcaccag tgcaatcccc aacaaaatgg cgctggccca taaaattcgc cgctcgctgc
    16321 gtttaatgac cggagccatc gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc
    16381 gcgagccgcc cataatcggc gcggcaacat acatcaccga actttcggga tcggcaggat
    16441 tttgcaacgt gctgcgcgca ccatactgac cacgcaacgt tagccagacg tcattccagc
    16501 gcgaataatc ctgtccaacg gctttatttg ccgaatcgaa caatactttg ccatgcgcat
    16561 cggtcatata gacatgatat tcattgcgca ctttgttaat gccaccaata ttggcgcgaa
    16621 acgggcgatg ttgtagctga ttaaacgcct gcgccagttg cccatgcgtt gggtccccag
    16681 agagcaaatc gggacgcgcc agctccgcaa gtaacgttgc cgtgtcgatc aacgtcccct
    16741 ccgttgctct tcgcacgccc ggtttaactt ctttgacaaa aatagccagt acgaaccagg
    16801 ccgccaccgc caccagtaaa aaatagccca gcagcaaccg catgccgata cgcattacaa
    16861 gcccctcagg ctatatccca tgccgcgatg agtattaatc ggtgaaagat cggggttgat
    16921 ggcgcgcagc ttggcacgca gcgttttaat gtggatatcg acggtgcgat cgtaggtgtc
    16981 ctgcgcatct tcccacacgc tatccattag ttgctggcgg gaccagacgc ggcctggcga
    17041 cttgagtaac gtcttcagca ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa
    17101 ccagctgatc tgcgccgcgg gttcattcaa ttcaaaatga ccaatacgga tgacgggaga
    17161 cggcgtcgag aacttcttca cccgacgcag taaggtgcgc accctggcgc acacttcgcg
    17221 gggtgaaaac ggtttagcca catagtcgtc agcgccaatt tccagcccaa gcaggcgatc
    17281 gacctcttca cttcgggccg tcaggaacag tacaggtaac gccggataga gcgccagtaa
    17341 ctggcggcac aattcaaagc cactgatatc aggcaaacca acgtcgagaa tcatgacgtc
    17401 gggaacctgc tggcgagctt tatccagcac cggcaaacct cgctcaaaga cctcgacggc
    17461 aaaaccttcc tgctgcaaca tgtagaccag cgtgtcggct atcccttgct catcttccac
    17521 taaccagacc gtttcccgtt gcataaatcg cctctgttat tgccgccacg gcatgacagg
    17581 aaccgcgcta atcgcgtttt tcggcgaacc ttctacaact ttgtcggagt aagccagata
    17641 agcgagcgcg ttgcgtttgg cgtcataaaa gcgcacgacc tgtaacgact taaagaccag
    17701 ggacgtgcgt tttttgaata ctacctcgcc ctgagctttg ccgtttttaa tacgatccga
    17761 cagttcaatc ggcccgactt gctgacaaga aatggccgca tcggaggtat cttctgccag
    17821 gcccaatccc cctttaatac caccggtttt cgcccggctc acataacagg tgacattttt
    17881 cacatcggga tcgtcaaagg cttccacaac aattttgtga tccgggccga tcattttaaa
    17941 tacggtgtcg accgaaccaa tctcttctgc atgagccaat ggccccagca ttatcaggct
    18001 taaagacagg atcaaatgct tgtatttcat attgttacca tttttatcta ttacacgagc
    18061 aattagttcg tcacggtaaa taacatattg attaagaaca aaatctcaat tcttttattt
    18121 ccgtcaagcc ctaaaacata ctctactaag aaaaaaacac tgaatgctaa aacagcaaaa
    18181 aatgctatta tccaattacc tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt
    18241 atggatcagg ccggcattat tcgcgacctt ttaatctggc tggaaggtca tctggatcag
    18301 cccctgtcgc tcgacaatgt agcggcgaaa gcaggttatt ccaagtggca cttacagaga
    18361 atgtttaaag atgtcactgg ccatgctatt ggcgcgtata ttcgtgctcg tcgtttgtcg
    18421 aaatcggcgg tcgcactacg cctgactgcg cgtccgattc tggacatcgc gctgcaatac
    18481 cgcttcgact ctcaacagac atttacccgc gcattcaaga agcagtttgc ccagactcct
    18541 gcactttacc gccgttctcc tgaatggagc gcctttggta ttcgcccgcc gctacgtctg
    18601 ggtgaattca caatgccaga gcacaaattt gtcaccctgg aagatacgcc gctgattggt
    18661 gttacccaga gctactcctg ttcgctggag caaatctctg atttccgcca tgaaatgcgt
    18721 tatcagttct ggcacgattt tctcggcaac gcgccgacca ttccgccggt gctctacggc
    18781 ctgaatgaaa cgcgtccgag tcaggataaa gacgacgaac aagaggtatt ctataccacc
    18841 gcgttagccc aggatcaggc agatggctat gtactgacgg ggcatccggt gatgctgcag
    18901 ggcggcgaat atgtgatgtt tacctatgaa ggtctgggaa ccggcgtgca ggagtttatc
    18961 ctgacggtat acggaacgtg catgccaatg ctcaatctga cgcgccgtaa aggtcaggat
    19021 attgagcgat actacccggc agaagacgcc aaagcgggag atcgcccaat taatctgcgc
    19081 tgtgaactgc tgattcctat ccgccgttaa cgctgcagct catctaatgc aggggcgtcc
    19141 agatgcgaga tgtcccctgc ggtttcaacg acccagccag acgccagcca caggctttcc
    19201 tgataatcca cgcgcgaaat agaacagtta cgcagacgta agcggcgctc tgcccatgct
    19261 ggtaatccga gaatcgtact caccagacat cccagtgcaa taccatggct caccagcagc
    19321 ggtcggcttc cctgcggtaa gtcccggcaa gattccagtg ccgcattgac gcgatcgctg
    19381 agctcctgca ttgactcgcc ttcaggaata cgcccgtcaa cggtgccatt gaccagctgc
    19441 cgacgccagt tctcttcttc ttcggtcaga gaatcgatat gtcttttttc cagcacaccc
    19501 atgtttaatt cacgcaggcg agaatcaaag atgatgtcac agccgcaggc ctgggcgatg
    19561 atttccgccg tacgccgggt gcgtcctaaa tcgctactga taatatgcgt aatgccaagc
    19621 tctttggcac gggttgccac ctgcatcgcc tgttgctcac ctttggcggt cagcgggctg
    19681 tcagactggc cctgaatacg tcgctcggcg ttccactgcg tttcaccgtg gcggactagg
    19741 tatacctgta acatgctttt tttccgtata ctgctgtaac gttaattttc tgagtaatgc
    19801 tgattatgca ccaagttgtc tgtgcgacca ccaatcccgc taaaattcag gccattctgc
    19861 aggcatttca cgagatcttc ggcgaaggat cctgccatat tgcatccgtt gccgtcgaga
    19921 gcggtgtacc ggaacaaccc tttggcagtg aggaaacgcg cgctggcgca cgaaatcggg
    19981 tagcaaatgc ccgccgttta cttccagagg ctgatttttg ggtggcgatt gaagctggca
    20041 ttgatggcga cagcactttc agttgggtgg tgattgaaaa cgccagccag cgcggcgagg
    20101 cgcgttcggc aactttgccg ttacctgcgg tgattctgga aaaagttcgt gaaggcgagg
    20161 cgcttggtcc cgtgagtcgc gttataccgg tattgatgag attggtcgta aggaaggggc
    20221 gatcggcgtg tttaccgccg ggaaactcac tcgcgccagc gtctatcacc aggcggtgat
    20281 cctggcgctg agtccgtttc acaatgccgt gtatcaggca ttgtaggacg gataaggcgt
    20341 tcacgccgca tccgacattc ggtgcacgat gcctgatgcg acgctgccgc gtcttatcag
    20401 gcctacaaaa tcaatcgctt ttcagcaaca cctcttccag ccactgacgc agctccacgg
    20461 gcgcggcttt caggctgtta gatccacgcg taatcgtcgc gatgcccgcg ccgagttcat
    20521 tttttaactc acgctgactc atttcgccac gcaacagctc ttcgacaata cgcacgcgag
    20581 tccccaacgc ttcgcgctca tctggcgtca gcatcaggtt taacaacggt aaatggagat
    20641 cgttttggta ggcattctta agcaggtcga caaaacgtaa ccactcctgg tgacgctgtt
    20701 ctgccatcgc tgctgaatag ggtgattgtt gggccataat atgtcgccat tgttagcggg
    20761 ggaagcaaaa tgactccccc ggttgtactc gctaaagagt acgatagcat atcataaacg
    20821 tgcggatcag taacgacgtc cccattccgt ggcgctcatc aacgtcggtt tatcccccat
    20881 gaaatagcgg tagtaagcat cataagccag cacgttcttc acataaccgc gcgtctcgga
    20941 gaacggaata ctctcgacaa atgccacagc gtcgatacgc ccggcgctgt tgccaagcca
    21001 ggttcgcacc cgtccaggtc cggcgttata agctgctgag gagaaaatac ggttattgcc
    21061 aaactgctga taaacatatt gcaggtaact ggtgccaatg ttgatgttcg tttccggatc
    21121 cagcaattgc ccagggctgc tataaccagg aatagagaac atcttcaccg tatgggtcgc
    21181 tgtaccaggc ataatctgca tcaggccgct ggcccctacc ggtgatttca ctttcggatt
    21241 ccaggcgctc tcctgacggg caatcgccat cgcatagctt tgcgggatct ctttcccact
    21301 ggtgtaacgt ttgaaaagat cgttgtaagc cagcgggaat cgctcttcca gatgatccca
    21361 aagcttcccg gcgatcgttg cctgaacgct aagatcccac cattggttat tgaaagcata
    21421 ccgagccagt tgagcctgct ctgtttttga cttgctcttc accagattgg cccactcgct
    21481 acgcgcggta ttatcgagat tccagtacat caactcgcgc acgcgcgcca tctccggccc
    21541 ctgagtcagg gtgctgtcaa cattctgcgg cgctttatca atcttcagct catactcttc
    21601 accgatgcgt tgtgctgcaa ccatcgggta gaaaccacgc tgttgtatga gttgatgcaa
    21661 aatctctttc gcttcagctt cacgtccgcg ttccagcaat aaatccgcct gccagtaacg
    21721 ccattcatct ttctcttttg cttccatcgg cagacgcgcc agccaggtat tcaggccgcg
    21781 acgatcgccg gtgccaagcg ccattcgcac acggcgttca atcaacgacg tggattgcga
    21841 gcgcataatg gcgtcatcgc gccatttcgc ctgctcatcg gtgacatcat tgcccatcaa
    21901 acgccaggcg acgatatcgc gcagctcctg aatctgatct tcattaagct gctgcgcctg
    21961 ggcaagcgac gggatcatca gccgtgcatt ttcggcatcc tgccgcgcca cactggcaaa
    22021 cgccaccgcc gccatttgac gggtaaaatc ggtcgcgccg gttgtacgcg cgaaggtcag
    22081 taccgtatta gggttgttcg ccagtgaaat gattgccgag gcgatagtct ggtaatcggc
    22141 aggcatctgc cctgccagca ctgttaccag gcctgtgtta cccgctttca tcgccagacg
    22201 gatacgctct aaatacgcca gcggatcttg tttacctgac gcacgccaga cgctaaataa
    22261 cttgtcacag gcgttaggct ggctcttgcc ggttagccac agctctttcg ccccttgcca
    22321 ggcttcttca ctctgcccgg tgttccattt cgcatagtag taattacatt gcgcttcggt
    22381 agttccgggc ttttccgggc taaaggctaa caagccacgc cagtcttcac gccgcgccag
    22441 ttcattgacg aaacgagatt gcagcgtgcg agcgggagga agcgtggggt tagcgcgaac
    22501 aaagttagtg accgtcaccg ccggttgatt catcaggtca tcggtgatct ggcggtattc
    22561 caggtaggga taaagcggat aatccttcag tccaggcatc atttgttcga ccacatccat
    22621 ctgtcgatta tcccaggcct gcttgatttg cgcgtaacgg ctacgctgct catccagtga
    22681 gtcggctcgc gccacgctgc tgaccgtcag cagacagaca ccggcagcca acagccgcca
    22741 ggtaacttgt ttggcttttt ccacaagcgc atcctctaag tgtaaataca tcaatgcagc
    22801 gtcatgccgc gtaatagatt tatgctaacc agtcattgcc gtttacgcca cgttacggac
    22861 acttttttac ttttactgcg aaggtgatcg accaactggg attaggcggg gaaacaggct
    22921 acacttcgcc tttgaaaact catcatcacg ataaacatag aggcgaagtc caacgtggct
    22981 caattcgttt ataccatgca tcgtgtcggc aaagttgttc cgccgaaacg tcatattttg
    23041 aaaaacatct ctctgagttt cttccctggg gcaaaaattg gtgtcctggg tctgaacggc
    23101 gcgggtaagt ccaccctgct gcgcattatg gcgggcattg ataaagacat cgaaggtgaa
    23161 gcgcgtccgc agccagacat caagattggt tatctgccgc aggaaccgca gctgaacccg
    23221 gaacacaccg tgcgtgagtc cattgaagaa gcggtttcag aagtggttaa cgccctgaaa
    23281 cgcctggatg aagtgtatgc gctgtacgcc gatccggatg ccgattttga caagctggcc
    23341 gctgaacaag gccgtctgga agagatcatt caggctcacg acggtcataa cctgaacgta
    23401 cagctggagc gtgcggcgga tgcgctgcgt ctgccagatt gggacgcgaa aatcgctaac
    23461 ctctccggtg gtgagcgtcg tcgcgtggcg ttgtgccgcc tgctgctgga aaaaccagac
    23521 atgctgctgc tcgacgaacc gaccaaccac ctggatgccg aatccgtggc gtggctggaa
    23581 cgcttcctgc acgacttcga aggcaccgtg gtggcgatta ctcacgaccg ttacttcctc
    23641 gataacgttg caggctggat cctcgaactg gaccgcggtg aaggaattcc gtgggaaggt
    23701 aactactcct cctggctgga gcagaaagat cagcgtctgg cgcaggaagc ttcacaagaa
    23761 gcggcgcgtc gtaagtcgat cgagaaagag ctggagtggg tacgtcaggg aactaaaggc
    23821 cgccagtcga aaggtaaagc acgtctggca cgctttgaag agctgaacag caccgaatac
    23881 cagaaacgta acgaaaccaa cgaactgttt attccacctg gaccgcgtct gggcgataaa
    23941 gtgctggaag tcagcaacct gcgtaaatcc tacggcgatc gcctgctgat tgatgacctg
    24001 agcttctcga tcccgaaagg ggcgatcgtc gggatcatcg gtccgaacgg cgcgggtaaa
    24061 tcgaccctgt tccgtatgat ctccggtcag gaaccgccgg acagcggcac catcactttg
    24121 ggtgaaacgg tgaaactggc atcggttgat cagttccgtg actcaatgga taacagcaaa
    24181 accgtttggg aagaagtttc cggcgggctg gatattatga agatcggcaa caccgagatg
    24241 ccaagccgcg cctacgttgg ccgctttaac tttaaagggg ttgatcaggg taaacgtgtt
    24301 ggcgaacttt ccggtggtga gcgcggtcgt ctgcatctgg cgaagctgct gcaggttggc
    24361 ggcaacatgc tgctgctcga cgaaccaacc aacgacctgg atatcgaaac cctgcgcgcg
    24421 ctggaaaacg ccctgctgga gttcccgggc tgtgcgatgg ttatctcgca cgaccgttgg
    24481 ttcctcgacc gtatcgccac gcacatcctg gactaccagg atgaaggtaa agttgagttc
    24541 ttcgaaggta actttactga gtacgaagag tacaaaaaac gcacgctggg cgcagacgcg
    24601 ctggagccga agcgtatcaa gtacaagcgt attgcgaagt aatgcgtaaa atgccggatg
    24661 cggcgcgaac gccttatccg gcctacaaaa ccagcaattt caatacgttg taaaactgta
    24721 ggcctgataa gacgcggtaa gcgtcgcatc aggcattgag caccgaatgc cggatgcggc
    24781 gtgaacacct tttccggtct acaacagcct tagcatcctc aatttcgtgg cgggtcaccc
    24841 gtgcaggttt tttcaaacct tgtttaatct cgacagcctc ttccagagag gttttcaact
    24901 catcaaaaaa actcatttta tacctcgcct ttgagcagtt tcgtcacggt tatccttgct
    24961 cccccatcat ctcccgcacc agctccacgc agcgcaggaa acggctgtcg taatcgtcct
    25021 cttcaacccg cacgaattcg atattgttct cttccagcat ctccaccagc aagttctgga
    25081 actctttgcg atccaccgaa ctgccgaggc tgcgtaaacc atccgccacc cacggcgtgt
    25141 tgttctccag caggatcacc agatcgaaac ggtattcatc aatcagcgcc tgcacgaacg
    25201 gatgctcacg cccttcgtac tttttgcaga acgcctgggt agtgacaaaa tcggtgtcaa
    25261 taaacgccac tttattggcg tatttcactg caaaatcaat gtattgtgcg tgacccaacg
    25321 cgattttatc gtagtcggaa tactgcaatg cgatttcatc accgccgagg tgtgaaaaga
    25381 cataatcgcg gccatattcc cacgcactgg tggtgttgaa gatattggca agtttgttta
    25441 ccagggtgga tttaccgctc gactcgccgc caaggatcgc cacggtacgc acaaaaaacg
    25501 gcttcacttc ggtaggaata tattcccagt agcggaacgg gttttcgcgg atctgcgcac
    25561 cgctgatact cataaaggta cgtttcggat cgaccagcac cgtctcgatc cccagatgtt
    25621 ccatatactg tggcgcatcg gcttcttccg aggtgtagat caggtccggc tggatcccct
    25681 tttcagccat aaactttttg atgccgttgc tccacacatc ccagccatgc ggatacggct
    25741 ccatgccctc ttcgttgaaa gcatgaatac ggatattttt ctgatattta aaggtttgca
    25801 atagccagcg cagacgatcc ggcaccgtgg gctgctgcga catggcgctg tcttcaaaca
    25861 acgcgcggtc gcgggtatcg tcaaaaccca taatgatatg cagctcgtca acctggctac
    25921 aggcgcgctg gataaggtag atatgtccgg tatgcagcgg gtagaactta ccgaatacga
    25981 caccaatggt tttcttctgc cggggaaatt caagccccaa aaaacggtgc aatgcctcca
    26041 gcttttgcgc gctggggctt ttaattttgg cattcagtaa ctggcttaaa taccctttgg
    26101 tcataccgct ggcatcagct acctgctgta gcgtgcagcc ctgttgcttg atggcagttt
    26161 tcaggtaatc aaatgacgac atatctccct ccgtatctct cattataagt cgtcgaacac
    26221 gctaagcgcg tcggagagtt ttttaacgcc aaaaatctgc atcccttccg gcgctttttt
    26281 cggtacgtta gccgccggaa caatcgcccg gcgaaaaccg tgtttcgccg cttcagaaat
    26341 ccgctcctgg ccactgggca ccgggcggat ctcccctgcc agcccgactt caccaaacac
    26401 caccagatcc tgtggcagcg gtctgtcacg caggctggaa accatcgcca gcagtaacgc
    26461 taagtcggcg ctggtttcgg ttaccttcac gccgccgacc acgttcacaa acacatcctg
    26521 atcggccatt tgcagaccac cgtgacggtg caagacagcc agcaggattg ccagacggtt
    26581 ttgttccagc cccactgcca cgcggcgcgg attcgccatt atcgagtgat cgaccagcgc
    26641 ctgaatctcc accagcaacg gacgcgttcc ttcccacacc accatcacgg agctgccgga
    26701 ggtcacttca tctccgcgac ttaagaaaat tgccgaaggg ttgctgactt cacgcagccc
    26761 ctgttcggtc atcgcgaaga cgcccagctc attcaccgcg ccgaagcggt ttttatggct
    26821 gcgtaaggtg cgaaaacggg agtcggcatc gccatccaga agcaccgaac agtcgataca
    26881 gtgttccagc actttcgggc cagccagcga accatctttg gttacgtgtc caaccatgac
    26941 aatcgccaca ccgcgcgttt tggcgaaacg cgtcagataa gccgccgttt cacgcacctg
    27001 cgccacgcta ccaggcgatg attgtacatc cgccatatgc atcacctgga tcgagtcaat
    27061 taccatcagc ttcggttgct cttcttcggc aatcaggcag atctgctcga tgctggtttc
    27121 cgacaacata ttgagattgt cagtcggcag gccaaggcga tgagcgcgca ttgccacctg
    27181 ttgcagcgac tcttcgccgg tgacatacag cgttttcatc tgctgggcca gtttgcacag
    27241 cgtttgcagc aacagcgtgg atttccccgc accagggtta ccgccaatca gaatggcact
    27301 tcctggcacc acgccgcctc ctagtacgcg gtcgaactct ttaaatccgg tggaaaaacg
    27361 cggcagctct tcaaggctga tatcggagag tttctggact tttgccaccc cggcgctacc
    27421 ggcatagccg ctgagacgct cgttacgcgc caccgttggc gacgcagcaa gacgcacctc
    27481 ggtgatggtg ttccaggcat gacaggcact gcactgcccc tgccagcgcg gataatcggc
    27541 cccgcattca ttacaaacaa aggcgcgttt tggagctttt gccacggtgt acctcgttaa
    27601 tgctgtgccg cccgcaggat gacgggcagg caattacttc tgattcaggc tgccagagag
    27661 gatgcagaat acccccatca ggtcagcgtg acggatggtg acttccgcct tttcattcac
    27721 ttttggcttg gcatggtagg caatccccag ccctgctgct ttgatcatcg gcaggtcatt
    27781 agctccatcg ccaatcgcca cggtctgcgc cagcgggatt tcatactcct gcgcgaggcg
    27841 agtcagagtt ttcgctttgt actgcgcgtc tacgatgtcg ccgatcacat tgccggtaaa
    27901 tttaccgtcc atgatctcca gttcattggc taccacggct gtcaggcgca gcttgtcgcg
    27961 caggtattca gcaaagaaag taaagccgcc ggaggcaatc gccactttcc agcccagcgt
    28021 ttccagcttg agcaccagct gcgttaagcc tggcatcagc ggcagatttt cacgcacctg
    28081 ttgcagaata ttggcgtcag cgcctttcag cgtcgccacg cggctgcgca ggctggcggt
    28141 aaaatcgagt tcgccgcgca tcgcccgttc ggttacctcc gccaccatct cgcccgttcc
    28201 ggccagcttg gcaatttcat caatacattc aatctggatg gcggtggaat ccatgtccat
    28261 caccagcaaa cccggcgtgc gcaggtgcgg gattttcccc agcggggcga catccagctg
    28321 cgcttcgtgg gccaggcgtg tagcccgtgc ggtgagtgaa cctgccagac gaatcacctg
    28381 ataatcttcc acgcaccagg cggcaacaat caccatcgcc gcacccagtt tgctctggta
    28441 ttgggtcaga cgctgtttat ccagcccacg accatacagc agccagccgc tacgacctgc
    28501 gtggtaatcc agtggcatca cttcatcacc acttaatgaa agaggcagac ccggccataa
    28561 agagacatct tcaggcaggt cgcaccaggt aatgttaggc attaaggctc ctgtaaaatc
    28621 gttcgaagca gggaaaataa cgcatgaggc taccttgtat ccattgcttc tggcaacatt
    28681 aagtctcaaa ttttcaaagg gtggaagatg gctcgcacaa aactgaaatt ccggctacat
    28741 cgggcagtga ttgtcctgtt ctgtcttgcc ttgttagtgg cactgatgca gggggcgtca
    28801 tggtttagtc aaaaccacca gcgacagcgt aatccacagc tggaagaact ggcccgcacc
    28861 ctggcgcgtc aggtgacgct gaacgttgca ccgctgatgc gtaccgactc accggatgaa
    28921 aaacgcattc aggcgatcct cgatcagtta acagatgaaa gccgtatcct cgacgcgggt
    28981 gtgtatgacg aacaaggcga tcttatcgca cgttctggcg aaagcgtcga agtgcgcgat
    29041 cggctggcgc tcgacggtaa aaaagcaggc ggctatttta accagcagat tgtcgagcca
    29101 attgcgggta aaaacggacc gctcggctat ctgcgcctga cactcgacac ccatacgctc
    29161 gccaccgaag cccaacaggt ggataacacc actaacattt tacgcctgat gttgctgctc
    29221 tcactggcaa tcggcgtagt gctgacccgc acgctgctac agggtaaacg cacccgctgg
    29281 cagcaatcgc ccttcctgtt aaccgccagc aaaccggtgc cggaagagga agaaagcgag
    29341 aaaaaagagt gacccattac tacaagaaag gaaatcgtta tgtccacatt acgcctgctc
    29401 atctctgact cttacgaccc gtggtttaac ctggcggtgg aagagtgtat ttttcgccaa
    29461 atgcccgcca cgcagcgcgt tctgtttctc tggcgcaatg ccgacacggt agtaattggt
    29521 cgcgcgcaga acccgtggaa agagtgtaat acccggcgga tggaagaaga taacgtccgc
    29581 ctggcgcggc gcagtggcgg tggcggcgcg gtgttccacg atctcggcaa tacctgcttt
    29641 acctttatgg ctggcaagcc ggagtacgat aaaactatct ccacgtcgat tgtgctcaat
    29701 gcgctgaacg cgctcggcgt cagcgccgaa gcgtccggac gtaacgatct ggtggtgaaa
    29761 accgtcgaag gcgaccgcaa agtctcaggc tcggcctatc gcgaaaccaa agatcgcggc
    29821 ttccaccacg gcaccttgct actcaatgcc gacctcagcc gcctggcaaa ctatctcaat
    29881 ccggataaaa agaaactggc ggcgaaaggc attacttcag tgcgttcccg cgtgaccaac
    29941 ctcaccgagc tgctgccggg gctcaccatg agcaggtttg cgaggccata accgaggcct
    30001 ttttcgccca ttatggcgag cacgtggaag cggaaatcat ctccccggac aaaacgccag
    30061 acttgccaaa cttcgccgaa acctttgccc gtcagagtag ctgagagtgg aacttcggtc
    30121 aggctccggc attctcgcat ctgctggatg aacgctttag ctggggcggc gtggaactgc
    30181 atttcgacgt tgaaaaaggc catatcaccc gcgcccaggt atttaccgac agcctcaacc
    30241 ccgcgccgct ggaagccctc gccgggcgac tgcaaggctg cctgtaccgc gcggatatgc
    30301 tgcaacaaga gtgcgaagcg ctgttggttg acttcccgga ccaggaaaaa gagctacggg
    30361 agttgtcgac gtggatagcg ggggcggtaa ggtaactcgc tgattaaaat atccaaggaa
    30421 ggaaatatga tcgatctgga aaaccaggaa cgggagatta tcaatttaat gttcagccag
    30481 cgtatttcct ggctagccgc cgtgcgtatc aggcataaat tatcgcttgc tgaagtaagt
    30541 aaaatgttag gtatctcgat taattctcta aaacagatcg aaaagacgga acggcttagc
    30601 tcaaacatca aaagcataat ggcagaaatt tatggttgtc caccagagtt gctgatatgc
    30661 ccttcctgga tgacggctga acataaatga atagattcgt gaatagatac ttgaatgttt
    30721 tttattttct atccatatga aaaataatag taaatctata aatcagttgc tattgatgaa
    30781 tagatactaa aaagccggag cagtccccca ctccggctta acacgacaat cgcctttcag
    30841 cgaaacacaa ttactcttta tcgcccagca gaacggattc cagtgcgatt ttgatcatgt
    30901 cgttgaaggt ggtctgacgc tcagcggcag tggtctgctc gtgagtgcgg atgtggtcag
    30961 acacggtgca gatggtcagg gctttcgcgc caaactctgc agccacgccg tagataccag
    31021 ccgcttccat ttccacgccg aggatgccgt atttttccat cacgtcgaac atttcgccgt
    31081 ccggagagta gaacaggtca gcggagaaca ggttacccac gcgagcatca acgcccagtg
    31141 ctttagctgc atctactgcg ttacgcacca tgtcaaagtc agcgatagcg gcaaagtcat
    31201 ggtctttaaa acggatgcgg ttaactttgg aatcggtgca ggcccccata ccgataacga
    31261 cgtcgcgcag ttttacgtgc ggcagaactg cgccacagga accaacgcgg ataattttct
    31321 tcacgccgaa atcggtgatc agttctttgg tgtagatgga gcaggacggg atacccatac
    31381 cgtgacccat tacggaaatt ttgcggcctt tgtaagtacc agtgaagccc agcataccgc
    31441 gaacgttgtt cacttcacgg gcatcttcaa ggaaagtttc agcaatatac ttcgcacgca
    31501 gcgggtcgcc tggcatcaaa actacgtcag cgaaatcgcc catttctgca ttaatgtgtg
    31561 gggtagccat tgttttatcc tttttgtgac ataacaaagg cggagtcaac gctccgccca
    31621 aatccatcag aacatggctt tgccatattc catatcagaa gtaccaaaat attttgccag
    31681 agtctggccg atatccgcga aggtttcacg gtgacccagt gagcccggtt ttactttcgg
    31741 gccatatacc agtaccggaa tgtgttcacg cgtgtggtca gtaccggtcc aggtcggatc
    31801 gcaaccgtgg tcagcggtga ggatcaggat gtcgtcatcg cgcagcagag acatcagctc
    31861 cggcagacgg cggtcgaaca gttccagacc cgcagcataa ccggcgacgt cgcggcggtg
    31921 gccccaggaa gagtcgaagt caacgaagtt ggtgaagacg atggtgttat cacccgcttc
    31981 tttcatctct ttgatggtgg cgtcaaacag cgcgtccaag ccagtcgctt tcactttttt
    32041 ggtgataccg cagttggcgt agatgtctgc aattttaccg acagaaacca cctggccgtg
    32101 tttttcatca accagtttct gcagcacggt cggtgctggc ggctcaacag ccaagtcgtg
    32161 acggttaccg gtacgctgga agttaccagc tttgtcaccg ataaacggac gagcgataac
    32221 acgaccgata ttgtagccgc cgttggtcag ctcttcacgg gcgatttcgc acagttcgta
    32281 gagtttatcc agaccgaaag tttcttcatg gcaggcaatc tggaacacgg agtcagcgga
    32341 ggtatagaaa atcggcttgc cggttttcat gtgctcttcg cccagttgat ccagaatgac
    32401 cgtaccggaa gagtggcagt taccgaggta acccggcaga ttagcgcgtt cgaccagttt
    32461 atccagcagc tcttgcggga agctgttttc gtgatcggag aaatatcccc actcaaacag
    32521 aaccgggaca ccggcgattt cccagtgacc agacggggta tctttaccgg atgacatttc
    32581 gtgcgcccat gcgtacgcgc cgataacttc agcgttgccg tccattcccg ccggaatgaa
    32641 accggtagaa ccttcgtgtg ctttcgccag ccccagacgg gtcagatttg gcagattgag
    32701 cggcccttta cgaccattat cagcttcgcc tttggcacaa gcttctgcga tatgacccag
    32761 ggtgtcagcc ccgacgtcac caaagcgttc tgcatcttct gtagcgccga tgccgaatga
    32821 gtccagcacc ataataaatg cacgtttcat atgttctccg tactcagtgc ttcaaattta
    32881 aatgatcaga tcagtatacc gttattcgct gatacggcga tagacagttg gtgtgctttc
    32941 cggtgcgtta tcggcaagtt taattgccgc tttcaccgct ttcgccgctt cctgccagct
    33001 gttttcgtct ttcgcgagga taaccgccag cggacgctga ccgtctacct ggtcgcccag
    33061 acgcgccata tcagtaaagc cgacgctgta atcgatggtg tcagatgcct gacgacgacc
    33121 gccgcccatt gcaactactg ccatccccag cgcgcgggta tccatttcac tgacaaaacc
    33181 ttcggtatca gcatagactg ctttcgtcag catcgctgtc ggcagatact tcgcgtagtt
    33241 ctcaacgaag tcggtcgggc ctttttgtgc cgctaccata cgaccaaaga cttctgccgc
    33301 tttaccgttg tccagcaccg cctgcaattt cgcgcgcgct tcggcgtcat ctttcgccag
    33361 tttgccggag atcaacatct ccacgcacag cgccatcgtg acatcaaaca gacgcgggtt
    33421 acgatactca cccgtcagga actgcaccgc ttcacgaact tcaaccgcgt tacctgcact
    33481 ggaggccagt acctgattca tgtcggtgag cagcgcggtg gtgcgcacgc cagcgccgtt
    33541 agccacgcca acaatcgctt cggcaagggc ttcagagagt tcgtaggtcg gcataaacgc
    33601 gccgctaccc actttcacgt ccatcaccag cgcgtccaga ccttccgcca gtttcttcgc
    33661 caggatcgag gcagtgatca gcgggatgga gtccacggtt gcggtaatat cacgggtcgc
    33721 gtagaaacgt ttatcagccg gagccagtga actggtctga ccgataatcg ccacgccgac
    33781 gtctttaata atttcgcgga aacggttgtc atccgggaaa atgtcgaagc cagggatgga
    33841 ttccagtttg tcgagcgtac cgccagtatg accgaggccg cgaccggaga tcatcggaat
    33901 atagccgccg caggctgcga ccatcggccc caacatcagc gaagtgacat cgccgacgcc
    33961 gccggtcgag tgtttatcaa caatcgggcc attcagatgc aggcttttcc agtcgagaac
    34021 ggttcctgaa tctcgcatcg ccatggtcag cgagacacgc tcaggcattg tcatatcgtg
    34081 gaagaaaatg gtcatcgcga gggcggcaat ctgcccttcg gaaatagtgt tgtcgcgaat
    34141 accgttgata aagaaacgaa tttcttcatc gctcagcgca tgaccatcac gttttttacg
    34201 aataatttct tgtgcgagaa acaaggtaac ctcccgagga aaagagtatg aggcggcttt
    34261 tgccggatgc gtctacgcct tatccggcct acgggtagat acgtctcgta ggcctgataa
    34321 gcgcagcgca tcaggcgtaa agcatcttac ttagtagctg ctggcgctct taccgtcgcc
    34381 gtggcccagc gctttcagca ggcttgccag caggctggaa gcaccaaagc ggtagtgacg
    34441 cgcatcagcc cagtcagcac cgaacagttc atctgcgatg gcgagatatt tctgcgcatc
    34501 ttccgcagta cgcacgccgc ccgccggttt gaaaccaacg gttttttcta cgcccatatc
    34561 acggatcact tccatcatga tgcgagcgct ttccggcgtc gcgttcacag ccactttacc
    34621 ggtagaggtt ttgatgaagt ccgcacccgc tttgatggag atttcagacg ctttacggat
    34681 cagcgcttcg tctttcagtt cgccggtttc gatgatcact ttcagcagta cattcgctgc
    34741 cgcgcaagcc tctttacagg ctttcaccag gtcaaaacca acctgctcgt cacccgccat
    34801 cagcgcgcgg tacgggaaca ccacgtcaac ttcatcagca ccgtaggcga ttgccgcacg
    34861 ggtttctgcc agcgcgattt cgatgtcgtc gttaccgtgt gggaagttgg ttaccgtagc
    34921 gatacggatt tccggggtgc cctgctcttt cagagttttg cgagcaatcg ggataaaacg
    34981 aggatagata cagatagcgg cggtattgcc gaccggagtt ttggcctgat gacacagagc
    35041 aattactttc tcgtcggtgt cgtcgtcatt cagggtggtc aggtccatca atttcagtgc
    35101 acgcaggctg cttgctttca gatcagtcat ttcattctcc tggcttgtcg ccaataaaat
    35161 tcaccttgcg agtttgttag tattctaaca tctactccgc aacacacttc gatacacatc
    35221 acaattaagg aaatctactt acaagtttgc atcactgtaa tgcgatctgg ttcaaataat
    35281 tcactttcaa atgaatgcgt cagtggtggc aaacgcatca ggatcaaagt gaacatcacg
    35341 aaacttctta caatggcgca tcaccttcgg cataaaagga aaagaagatg cccacttctc
    35401 atgaaaatgc actgcaacaa cgttgccagc aaattgtcac cagcccagtg cttagcccgg
    35461 agcagaagcg ccattttctg gcactggaag cagaaaacaa tctgccttac ccacagcttc
    35521 ctgccgaagc ccgccgcgcg ctggatgaag gtgtaatctg cgatatgttt gaaggtcatg
    35581 cgccgtacaa accgcgctat gtcttacccg attacgctcg ttttctggcg aacggttccg
    35641 aatggctgga gctggaaggc gcgaaagatc ttgatgacgc actctctctg ctgaccattc
    35701 tttaccacca cgtaccgtcg gtcacatcga tgccggtcta cctggggcaa ctggatgcgt
    35761 tgttgcaacc gtatgttaga attctaacac aaaacgagat cgatgttcga ataaaacgtt
    35821 tctggcgtta cctcgacaga accctgccag atgcctttat gcacgccaat atcggcccgt
    35881 ctgattcgcc cattacccgc gcgatcttac gtgcagacgc gcaggtttca ccgaacctga
    35941 cctttatcta cgatcctgaa atcacccctg atgacctgct gctggaagtg gcgaagaaca
    36001 tctgtgaatg tagcaaaccg cacatcgcca acggtccggt gcatgataaa attttcacaa
    36061 aagggggcta cgggattgtg agctgttaca actcactgcc gctggcgggt ggtggcagca
    36121 cgctggtacg ccttaacctg aaagccattg ccgagcgcag cgaatcgctg gatgacttct
    36181 ttacgcgcac tctaccgcac tactgccagc agcagatcgc catcatcgat gcgcggtgtg
    36241 aattcctcta tcaacaatca cacttctttg agaatagctt cctggtgaaa gaagggctga
    36301 ttaaccctga acgttttgtg ccaatgtttg gcatgtatgg gctggcggaa gcggttaact
    36361 tgctgtgtga gaaagaaggg attgccgcgc gctacggtaa agaagccgcc gcaaatgaag
    36421 tgggttatcg catcagcgcg caactggcgg agtttgtcgc caataccccc gtgaaatatg
    36481 gctggcaaaa acgcgccatg ttacacgcac agtcggggat cagttccgat atcggcacca
    36541 cgccgggcgc gcgtttgccg tatggcgatg agccagatcc gatcacccat ctgcaaactg
    36601 tcgccccgca tcatgcttat tattattccg gcatcagcga cattctgacg ctcgacgaaa
    36661 ccatcaaacg taacccgcag gcactggtac agctttgcct cggtgccttt aaagccggaa
    36721 tgcgtgaatt taccgccaat gtcagcggta acgatctggt tcgcgttacc ggttatatgg
    36781 tgcgtttgtc ggatttagaa aaatatcgcg ccgaaggttc acgcaccaac accacctggc
    36841 tgggcgaaga agccgcacgc aacactcgta ttctggaacg ccagccgcgc gtgataagcc
    36901 atgaacagca gatgcgcttt agtcagtaag attatcccct tctcctgcgt tgacgggcca
    36961 ggcagtcgtc tggctctgtt tttgcagggc tgcaatctgc gctgcaaaaa ctgtcacaat
    37021 ccgtggacga tgggacgttg caatgactgt ggggagtgcg tgccacagtg tccgcatcag
    37081 gcgttgcaga ttgttgacgg caaagtggtg tggaacgctg tggtctgcga gcaatgtgat
    37141 acctgcctga agatgtgtcc gcaacatgcc acaccaatgg cgcaatccat gagcgtggac
    37201 gaagtgctta gccatgtgcg caaagcggtg ctgtttattg aagggataac ggtaagcggc
    37261 ggcgaagcca cgacccaact gccgtttgta gtggcgctgt ttactgctat caaaaacgat
    37321 ccgcaactgc gccatctcac ctgtctggtg gacagtaacg gtatgttgag cgaaaccggc
    37381 tgggaaaaat tactcccggt gtgtgacggc gcaatgctcg atctcaaagc atggggtagc
    37441 gaatgtcatc aacaattaac cggacgcgat aatcaacaga ttaagcgcag catctatttg
    37501 ctggcagagc gcggcaagct ggcggaactg cgtttgctga tgatcccagg ccaggtggat
    37561 tatttgcaac acatcgaaga actggcggcg tttatcaagg gacttggtga tgttccggtt
    37621 cgcctgaacg cgtttcatgc ccacggcgtg tatggcgagg cgcaaagctg ggcgagcgcc
    37681 acgccggaag acgttgagcc gttggctgat gcattaaagg tgcgcggggt gagccggttg
    37741 atatttccgg cgctctattt gtgacatgcc ggatacggcc tacggttcgg cacagacttg
    37801 taggcctgat aagacgcgtc aagcgtcgca tcaggcattg tgcgccaact gccggatgcg
    37861 gcgtgaacgc cttatccggc ctacggttcg gcacaaacct gtaggcatga taagacgcgt
    37921 taagcgtcgc atcaggcatt atgcgccgac tgccggatgc tgcctacgaa gggcaaacta
    37981 cccacgaacg ttaaacaccg catacgtgtt attaagcaac acttcggcaa tctcatccgc
    38041 gggttccggg cgtaactcgc aaagcacggc aaacacacgg gcagcctgtt ccgggcggtt
    38101 aggctgcccc tgaaaaccgt tgagcggcat atccggcgca tcggtttcca gcaacaacga
    38161 tgccagcggt aattttgcga tgacatcgcg ggttttactg gcgcgtggat aggtgatagt
    38221 accgcctacg ccgattttgt agcccagctg tacaaaccgt tcagcctgtt gctggctgcc
    38281 agaaaatccg tggacaacac cagcacgcgg caaatcgtgg cgtttaagat gcatcgccag
    38341 tttgtcgtgc gtgcgccgtg aatgcaggat caccggcaga tcgtagcgtt tcgccagttt
    38401 cagttgttcg tcgagtaacc actgctgcct ctcaaattgc ggatcgtcgc cgaagagatc
    38461 cagaccgatc tcccccaccg ccaccacctt cgccggacgc ctttccagcg cctgctgtag
    38521 ctgctcaaga gacacatcgc tatgtttttc caacatacca ggatgcaagc ccaatacggc
    38581 atacagcggt tgataatttt ccgctaacgc ctgcacacgg gcaaaatttt ccgcctctgt
    38641 cgccggaaca atgatcttgc ctacgcccgc ttgtgccgcg cgttgcaggc tggcctcttc
    38701 atcgccaata aaaggcggga aatcaaaatg gcagtgagtg tcgataaaac ggcaaatcaa
    38761 gccagatcct cattgttaaa tgtcgggtcg ttcgcctgcg gagcgtcgct cacttcggca
    38821 accagcgtat cgttggcgac tggcgcaggc ggaatgacaa tcgattccgg cgtcaccact
    38881 ggcaccagat ggcgggtaag cggcgctttt tcagttaata gcttgccaac cgtggcgagg
    38941 aaataacgac cgcataaacg cccaagttta tagtcttcac gcagcgccgg aatccgactg
    39001 ccaagcgcga tactatgtaa tggcttcggc ggataaattt cggatatccg cagcttgccc
    39061 ggtggtttct caataaattg ctgaatgtca cgatagctgg tttcatgatg ctgcaccaga
    39121 ttgaccagcg gctgcaggct actgtcaccc agccagcgtt ccatgcgttt gaaccactgc
    39181 ggcgtgtagt acatttgtga cggcacagtg cggatgacga ccaacgtttt agcgccctgc
    39241 cttgccgcct ctttaaccgg aatcgcatca ctgatcccgc catccaggta gttaatgcct
    39301 tccagcgaca ctccgctacg ataaaagcca ggtatcgcac tggaggcgcg aatcacatcc
    39361 agccagtttt gtttggttgg taaaaagtaa ttcggcgcgt agtcatcctg acgacaggcg
    39421 cacatataaa acgatttgcc gctgtcaaac aaccgcgcgg cggtatccat ttgcaacggc
    39481 atctggcttg cggtggcctc caccagccag tcgagatcga taagatttcc tccacggaca
    39541 aagcgcaatg gatcgaaaaa ttcgcgtttt gtggtatagc gcatgatgac tttgcgcgcg
    39601 taaccgggct gattgcagat aaacgccgag aggttctgcg ccccggcaga tgtgccgaga
    39661 taaagatcga aaggattaaa ctgcgcgcgc ataaactcat ccagcacgcc agccgtgaaa
    39721 attccacgct gtccgccgcc ttcgcacacc agagctattc gtccaggctg gaacggcctt
    39781 agcgacaacg gcgcaatatt accaagcgtt acaggtattc gctgccccac ttctgccttc
    39841 ctgtttttgt tgtgaaagaa agacaaagta acgcaattta ttattcgcta aaaccgcaaa
    39901 agccagtccg ctggactggc tttggataaa attttcttaa tggtctgaaa tctagggtcg
    39961 ttttcggccc atgaacaaac tcactaggaa cagaataatc ccgacgacaa agacaatttt
    40021 agctgcgcct gcagcggtac cggccagacc accaaaccca agtgcggcgg cgattaacgc
    40081 gataaccaga aatatgatgc cccaacgaaa cataagcgtc tcctttacca tagttaatgt
    40141 caccgcttga tatgagcgtg aaaatcactc aatgcgatat ttttagtgtg gtgcacatcg
    40201 cgcctcccga caaagttcgg gaggacgaat tacttagttt tcagatcatt tttaacgctt
    40261 ttcacaccat ctaccgcttt ggcgatactt tcagcacggt cactttgtgc ctgagaatct
    40321 acggtaccgg agagctgaac cacgccatcg gtggtttcaa ctttcacttt acgtgaaggg
    40381 acgatatcgt ccgccagcag tttggctttg atttcactgg tggtggcggt gtcacccgcg
    40441 tagcccttca ccgagccttc tttagcgtcg cgaacgtgca gtttgtcgct gacagaggtc
    40501 acgccttcaa cgcctttcgc cactttcact gcctcttcgg cctgggcctg actttcaacg
    40561 aaaccgctca gggtcacgac tttttgatcg gttttgacag agatatcggt gctcttgatg
    40621 ttgtcatgat ccaccagcgc cgccttcact ttcgcggtga tggcgctgtc atccatgaaa
    40681 ttaccgactt tattcataga gctatcgact ttttgccctg cgctttcatt ggtagtctgc
    40741 gcgttgtttt ccgcgtaggc agagccggtc gcgacggcag aggtcaacat tacagccagc
    40801 agagttttcg aaatcttcag tcttgtcata gtcatcgatt tattcctgta tgtttgctcg
    40861 taatttgagc ctagcaacac gaggttgcat tgctgaatag ggagagactt caccctctac
    40921 agaagtcaat ggtcgccatc acaaaagcga tgggtgatga ataacgacca ttacagcctc
    40981 tgaatcagtt attaatatcg gtagaatgac aatcgacggc tttagatact aatatctacg
    41041 cattgaacgg tatttaacgc catcagaaat gtcatcactt tgttaaatat agatcacaat
    41101 tttgaaaccg ctcgggatat cagcgaaaac ataagcaaaa gtgaatgttt taagaacatt
    41161 ccgtaagcgg ctaataagga agggaaattg acagggcgca gcggctaccg cgccctggag
    41221 gcaagaatta atgctcgcgg gtctggtgga actgaacgtc cggataacgt tcctgtgcca
    41281 ggcgcaggtt gaccatgctg gtagcgatgt aagcgaggtt atcgccgcca tcaagcgcca
    41341 gttggctttc gttcttacgc ttgaactctt cgaatttctt cgcgtctgca cattctaccc
    41401 agcgggcagt ggcaacgttg actgactcat acactgcttc aacgttgtat tcgctcttca
    41461 ggcgcgctac caccacatca aactgcagca caccaaccgc accaacgatc agatcgttgt
    41521 tggagattgg acggaacacc tgcaccgcgc cctcttcgga aagctgtacc agccctttga
    41581 gcagctgttt ttgcttcagc ggatctttca ggcggatacg acggaacagt tctggtgcga
    41641 agttcggaat accggtgaac ttcatcatct caccctgggt aaaggtgtcg ccgatctgaa
    41701 tggtgccgtg gttgtgcagg ccgaggatat cgcccggata cgcttcttca acgtgcgaac
    41761 ggtcacccgc cataaaggtc agcgcgtcgg agatcaccac atctttcgca gtgcgcacct
    41821 ggcgcagttt catgcctttt tcatatttac cggacaccac acgcataaac gccacgcggt
    41881 cgcggtgttt cggatccatg ttggcctgaa ttttaaatac gaagccggta aatttatctt
    41941 cgctcgcttc tacggtacgg gtatcggtct gacgcggcat cggcgcaggc gcccactcca
    42001 ccaggccatt cagcatatga tcaacgccga agttacccag cgcagtaccg aagaataccg
    42061 gagtgatttc gcccgcaagg aacagctctt tgtcgaactc gttagacgcg cctttaacca
    42121 gttccagttc gtcacgcagc tgctgtgcca gatcttcacc aaccgcagca tcgagatccg
    42181 ggttattcag ccctttaaca atgcggactt cctgaatggt gtgcccttta ccgctctgat
    42241 agagataggt ttcatcttta taaaggtggt aaacgccttt aaacagcttg ccgcagccaa
    42301 ttggccaagt gatcggtgcg cagccaattt tcagctcgtt ctcaacttca tcgagcagtt
    42361 ccatcgggtc gcggatatca cggtcaagtt tgttcataaa ggtgaggatc ggcgtgtcgc
    42421 gcagacgggt aacttccatc agcttacggg tacgatcttc aacacctttt gcggcgtcga
    42481 taaccatcag gcagcagtcc accgccgtca gggtacgata ggtatcttcc gagaagtctt
    42541 cgtgccccgg ggtgtcgagc aggttaacca ggcaatcgtg atacggaaac tgcatcacag
    42601 acgtagtaat ggagatccca cgctgctttt ccatctccat ccagtccgac ttagcgtgct
    42661 ggttggaacc acggcctttt actgtaccgg cggtctgaat ggcctgtccg aacagcagca
    42721 ccttctcggt gatggtagtt ttaccggcgt ccgggtgaga aataatggca aaagtgcggc
    42781 gcttcgccac ctctttcaaa taaggagaca acgtcataat tctttcttct tgagtaagcg
    42841 gcatcgcgcc gcgcttgttg aaaacgaaaa attgcggcta ttttacccat caacgggggg
    42901 gaggcaatca gtgtttacac aggagctgct ccagttcgtg caacgaagaa acggtccagg
    42961 tgggcgcgat gccttctggt tgctcgcgat tgtgcgcatt cagccagcag gtcgcaagcc
    43021 cggcgttgat gccaccgaga atatcggact cggcagtgtc gccaaccatc agcacgcgtg
    43081 aacggtcagg attgcccgcc tgttccagcg cataatcgaa aattttctta ttcggtttgg
    43141 caacgccaac ttcttcggaa atcaccagca aatcgaagta atcacgcagg cccgtccgtt
    43201 ccagacgcac ttgctgcaag gcgctaaaac cgttggtgat gatgccgatt ttagcgttgc
    43261 tacgaatggc gttaagcaga gaaaccgcgc ccggcaacgg cgtacagatt tccgccatcg
    43321 cattaataaa ggcatcgtta agcaagcctg cttcaacttt cagccgttcg gcccagctct
    43381 cgaaacgccc gtgctgaagc tgtaatgaag tgatcgcgcc gttttgataa tccacccaca
    43441 gtggcttgtt aacggcctga tagtcctgaa aatcttcagc ggtaaaggtg acgctgtaat
    43501 caagaaacat ccgctgcagg ccggtgaatg agtcaaaggt aaacagcgtt tcatcggcat
    43561 caaagaaaat ccagtcccac ttcattattc caccttgtct tacatactga ttggcaacgc
    43621 catgatgatg gcgtcttcgc gaccgtccgt ggtggggtag taattgcggc gaatcgtcgc
    43681 ctcgttaaag cctaaacttt cgtacagggc aatggcggca gcatttgagg cacggacttc
    43741 cagccatagt gtcgccacgc cgcgtttttc cagttcgtcg atcagatgtt ccagcagcgc
    43801 cctacccagc ccctgacgct gatagtcggg atcgaccgca atattgaaca atgtagcttc
    43861 atccagcacc acttgcgtaa tcgcaaacgc cgccattttg ccgttttgcg ttaactgaaa
    43921 gttgagataa cgctcgccct ggttgctggc aaaggttttt tcactccacg gaaaggcgtg
    43981 ggcgcgttgt tcaatgtggt aagccgctgg taaatcagtc gtttcgaggg aagaaatcgt
    44041 gttcatatgt gcaaatttgt tgccataacg cggcgcgtgc cgttgggttt gcccgtaatt
    44101 cggtgagcgc cggtgatgcc acctgagcgc cttccaatga tagcggttcg tcagtaccca
    44161 accgccagct gttgcagcga ctgccttgcg gcagcatcgc gattttttct ggcgtcagtt
    44221 gcagcacttg atcggggctg acggttaatg cgcgcagaac atcgctcact aaaggatcag
    44281 tcagggcggg aagatcgttt gccaccatca ccagacggac gtgtgccggg ctcgcaatgg
    44341 caatctcccc ctgcaacgcg ccagggcgac gcagcgacca ctgggtaatg cccagttgct
    44401 gtaactgcca gtctcgtcgg gatgtcatag cgaatcgctc ctgttgtcag gggcgcaaat
    44461 atagcaaatt cgtcgatacc gcgccaacat atggctataa tcgccgccag tatcaattga
    44521 ggagcattcc atgtctgcat ttaccccggc aagtgaagtc ttgctgcgtc acagtgatga
    44581 tttcgaacaa agccgtattc tgtttgccgg agacttacag gatgacctgc ccgcgcgttt
    44641 agataccgcg gccagccgtg ctcataccca gcaattccac cattggcagg tgttaagctg
    44701 ccagatgggg gataacgccc gttttagtct ggtcgccacg gcgaatgatg tcgcagattg
    44761 cgatacgctg atttactact ggccgaagaa caaaccggaa gcccagttcc agttgatgaa
    44821 tttactttct ctgctgccag tggggacaga tatttttgtc gttggcgaga accgcagcgg
    44881 cgtgcgcagc gctgagcaga tgctggcaga ttatgcgccg ttgaataaag tcgacagcgc
    44941 tcgtcgctgt ggcctctatt ttggtcgtct ggaaaaacag ccagtatttg atgcggataa
    45001 attctggggc gaatacagcg tcgatggcct gacggtcaaa acgctgcctg gcgtgtttag
    45061 ccgcgacggt ctggatgtcg gtagccagtt gctgctctcg acgttaactc cgcacacgaa
    45121 aggtaaagtg ctggatgtcg gctgtggcgc gggtgtactt tcagttgcct ttgcgcgcca
    45181 ttcgccgaaa attcgtctca ccttgtgcga tgtctctgcc ccggcggtag aagccagccg
    45241 cgcaacactt gcagccaacg gtgttgaagg tgaagtcttt gccagcaacg tcttttccga
    45301 ggtgaaaggt tgttttgata tgatcatctc caacccgccg ttccacgatg ggatgcaaac
    45361 cagcctggat gcggcgcaaa cgctgattcg tggcgcggtg cgtcatctta atagcggcgg
    45421 cgagctgcga attgtagcga acgccttcct gccttacccg gacgtgctgg atgagacatt
    45481 tggcttccat gaagttatcg cgcaaaccgg gcgcttcaag gtatatcgcg ccattatgac
    45541 ccgccaggcg aagaaaggtt gagtatctga tatctgtcgc cgcataccgt gtcggatgcg
    45601 gcgtgaacgc cttatccgac ctacaaaacc tgcacgttag cccttcgtag gccagataag
    45661 acgcgccagc gtcgcatctg gcatctgccc ccgcatgcct accaaacctg cacgctagtt
    45721 tcctgatgga catttttcca gcaattacac atcaggcgat aattaactat tgacgaaaag
    45781 ctgaaaacca ctagaatgcg cctccgtggt agcaattctt tttaagaatt gatggtatgc
    45841 gaaggtggcg gaattggtag acgcgctagc ttcaggtgtt agtgtcctta cggacgtggg
    45901 ggttcaagtc ccccccctcg caccaaaaat cacgttgata ttgctcgcac tgggcgaagg
    45961 tggcggaatt ggtagacgcg ctagcttcag gtgttagtgt tcttacggac gtgggggttc
    46021 aagtcccccc cctcgcacca aacgaggcga tatcaa
//
LOCUS       DAZSNM010000009        45809 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZSNM010000009 DAZSNM010000000
VERSION     DAZSNM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45809
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.009"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..819)
                     /gene="gcvA"
                     /locus_tag="ACHW42_000435"
     CDS             complement(<1..819)
                     /gene="gcvA"
                     /locus_tag="ACHW42_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR8942401.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHD"
     gene            complement(1170..1397)
                     /locus_tag="ACHW42_000436"
     CDS             complement(1170..1397)
                     /locus_tag="ACHW42_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR8942402.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            1589..2794
                     /gene="csdA"
                     /locus_tag="ACHW42_000437"
     CDS             1589..2794
                     /gene="csdA"
                     /locus_tag="ACHW42_000437"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR8942403.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            2794..3237
                     /gene="csdE"
                     /locus_tag="ACHW42_000438"
     CDS             2794..3237
                     /gene="csdE"
                     /locus_tag="ACHW42_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR8942404.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(3288..4094)
                     /gene="tcdA"
                     /locus_tag="ACHW42_000439"
     CDS             complement(3288..4094)
                     /gene="tcdA"
                     /locus_tag="ACHW42_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR8942405.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(4332..5429)
                     /gene="mltA"
                     /locus_tag="ACHW42_000440"
     CDS             complement(4332..5429)
                     /gene="mltA"
                     /locus_tag="ACHW42_000440"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR8942406.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDVKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            5638..5714
                     /locus_tag="ACHW42_000441"
     tRNA            5638..5714
                     /locus_tag="ACHW42_000441"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5672..5674,aa:Met,seq:cat)
     gene            5748..5824
                     /locus_tag="ACHW42_000442"
     tRNA            5748..5824
                     /locus_tag="ACHW42_000442"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5782..5784,aa:Met,seq:cat)
     gene            complement(5899..7152)
                     /gene="amiC"
                     /locus_tag="ACHW42_000443"
     CDS             complement(5899..7152)
                     /gene="amiC"
                     /locus_tag="ACHW42_000443"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR8942407.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            7384..8715
                     /gene="argA"
                     /locus_tag="ACHW42_000444"
     CDS             7384..8715
                     /gene="argA"
                     /locus_tag="ACHW42_000444"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR8942408.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(8777..10603)
                     /gene="recD"
                     /locus_tag="ACHW42_000445"
     CDS             complement(8777..10603)
                     /gene="recD"
                     /locus_tag="ACHW42_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR8942409.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(10603..14145)
                     /gene="recB"
                     /locus_tag="ACHW42_000446"
     CDS             complement(10603..14145)
                     /gene="recB"
                     /locus_tag="ACHW42_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR8942410.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(14138..17026)
                     /gene="ptrA"
                     /locus_tag="ACHW42_000447"
     CDS             complement(14138..17026)
                     /gene="ptrA"
                     /locus_tag="ACHW42_000447"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR8942411.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(17202..20570)
                     /gene="recC"
                     /locus_tag="ACHW42_000448"
     CDS             complement(17202..20570)
                     /gene="recC"
                     /locus_tag="ACHW42_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR8942412.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(20583..20933)
                     /locus_tag="ACHW42_000449"
     CDS             complement(20583..20933)
                     /locus_tag="ACHW42_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR8942413.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(20918..21325)
                     /locus_tag="ACHW42_000450"
     CDS             complement(20918..21325)
                     /locus_tag="ACHW42_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR8942414.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(21322..21885)
                     /locus_tag="ACHW42_000451"
     CDS             complement(21322..21885)
                     /locus_tag="ACHW42_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR8942415.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(21876..22346)
                     /locus_tag="ACHW42_000452"
                     /pseudo
     CDS             complement(21876..22346)
                     /locus_tag="ACHW42_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(22530..23324)
                     /gene="thyA"
                     /locus_tag="ACHW42_000453"
     CDS             complement(22530..23324)
                     /gene="thyA"
                     /locus_tag="ACHW42_000453"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR8942416.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(23331..24206)
                     /gene="lgt"
                     /locus_tag="ACHW42_000454"
     CDS             complement(23331..24206)
                     /gene="lgt"
                     /locus_tag="ACHW42_000454"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR8942417.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(24357..26603)
                     /gene="ptsP"
                     /locus_tag="ACHW42_000455"
     CDS             complement(24357..26603)
                     /gene="ptsP"
                     /locus_tag="ACHW42_000455"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR8942418.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(26616..27146)
                     /gene="rppH"
                     /locus_tag="ACHW42_000456"
     CDS             complement(26616..27146)
                     /gene="rppH"
                     /locus_tag="ACHW42_000456"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR8942419.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(27499..27645)
                     /gene="ygdT"
                     /locus_tag="ACHW42_000457"
     CDS             complement(27499..27645)
                     /gene="ygdT"
                     /locus_tag="ACHW42_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR8942420.1"
                     /translation="MLSTESWDNCEKPPLLFSFTALTCDETPVFSGSVLNLVAHSVDK
                     HGIG"
     gene            27831..28520
                     /gene="mutH"
                     /locus_tag="ACHW42_000458"
     CDS             27831..28520
                     /gene="mutH"
                     /locus_tag="ACHW42_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR8942421.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            28589..29302
                     /locus_tag="ACHW42_000459"
     CDS             28589..29302
                     /locus_tag="ACHW42_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR8942422.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            29440..29658
                     /gene="ygdR"
                     /locus_tag="ACHW42_000460"
     CDS             29440..29658
                     /gene="ygdR"
                     /locus_tag="ACHW42_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR8942423.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            29766..30806
                     /locus_tag="ACHW42_000461"
     CDS             29766..30806
                     /locus_tag="ACHW42_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR8942424.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(30839..32029)
                     /gene="lplT"
                     /locus_tag="ACHW42_000462"
     CDS             complement(30839..32029)
                     /gene="lplT"
                     /locus_tag="ACHW42_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR8942425.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(32022..34181)
                     /gene="aas"
                     /locus_tag="ACHW42_000463"
     CDS             complement(32022..34181)
                     /gene="aas"
                     /locus_tag="ACHW42_000463"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR8942426.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            34767..35798
                     /gene="galR"
                     /locus_tag="ACHW42_000464"
     CDS             34767..35798
                     /gene="galR"
                     /locus_tag="ACHW42_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR8942427.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(35805..37067)
                     /gene="lysA"
                     /locus_tag="ACHW42_000465"
     CDS             complement(35805..37067)
                     /gene="lysA"
                     /locus_tag="ACHW42_000465"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR8942428.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            37189..38124
                     /gene="lysR"
                     /locus_tag="ACHW42_000466"
     CDS             37189..38124
                     /gene="lysR"
                     /locus_tag="ACHW42_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR8942429.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(38111..38803)
                     /gene="ygeA"
                     /locus_tag="ACHW42_000467"
     CDS             complement(38111..38803)
                     /gene="ygeA"
                     /locus_tag="ACHW42_000467"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR8942430.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(38932..40350)
                     /gene="araE"
                     /locus_tag="ACHW42_000468"
     CDS             complement(38932..40350)
                     /gene="araE"
                     /locus_tag="ACHW42_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR8942431.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(40665..41426)
                     /gene="kduD"
                     /locus_tag="ACHW42_000469"
     CDS             complement(40665..41426)
                     /gene="kduD"
                     /locus_tag="ACHW42_000469"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR8942432.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(41456..42292)
                     /gene="kduI"
                     /locus_tag="ACHW42_000470"
     CDS             complement(41456..42292)
                     /gene="kduI"
                     /locus_tag="ACHW42_000470"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR8942433.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(42579..43760)
                     /locus_tag="ACHW42_000471"
     CDS             complement(42579..43760)
                     /locus_tag="ACHW42_000471"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR8942434.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            44015..45244
                     /locus_tag="ACHW42_000472"
                     /pseudo
     CDS             44015..45244
                     /locus_tag="ACHW42_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            45671..>45809
                     /locus_tag="ACHW42_000473"
     CDS             45671..>45809
                     /locus_tag="ACHW42_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8942435.1"
                     /translation="MQLYTDREVLNMGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCA
                     NS"
BASE COUNT        11350 a        12374 c        11140 g        10945 t
ORIGIN      
        1 gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa acgggcaaac
       61 aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg ccactccctg
      121 cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc cttgctgaac
      181 gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc gtgacgcatc
      241 atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt cgcctgtcag
      301 cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca cccgtagccc
      361 cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca gcttatcttc
      421 ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat taaagctgga
      481 aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg tcaacgcccc
      541 cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa atatctcttt
      601 gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat tacggcggcg
      661 gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta ctgcggcctg
      721 ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg cggcatcaaa
      781 aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac tttgatgtta
      841 aatgaattta acaattagat cacactgtgt aacctattag tttttttaat ctgagccatt
      901 ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact tcctgagccg
      961 gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg gttgtgatgt
     1021 tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag ctaccttttt
     1081 tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc gcctaattgc
     1141 ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat ctctttcacg
     1201 tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat cataccgata
     1261 tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt ggtgtgcatc
     1321 acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc agaaataatt
     1381 gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa tagtattgat
     1441 taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc ggcttcgcca
     1501 ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa ttttgcgtca
     1561 cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc agtttcgcgc
     1621 ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga ccgcgcttaa
     1681 acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg gaaacgtcca
     1741 tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg cacgagagaa
     1801 agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc gcggcaccac
     1861 tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac cgggcgatga
     1921 gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga tggtcgccca
     1981 acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc cggatgtcga
     2041 tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc agatgtcgaa
     2101 cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt cagccgggat
     2161 ggtggtgatg gttgatggtg ctcaggggac ggtgcatttc cccgcggatg ttcagcaact
     2221 ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg gtatcggcgt
     2281 gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg gcggcaaaat
     2341 gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga aactggaagc
     2401 aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat ggctggcaga
     2461 ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg cagaagaagc
     2521 gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc tgctggcctt
     2581 tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt acggtattgc
     2641 cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg taaccggcac
     2701 actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc tggtgaatgc
     2761 cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt cgccggacat
     2821 ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc gttgtcgcaa
     2881 tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc tttgccagac
     2941 gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg gctgggatat
     3001 accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg cattgtgcgc
     3061 ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga attacaggca
     3121 cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag cgcctcacgc
     3181 agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca ggtttaagca
     3241 cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta accctgacgc
     3301 gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc aaaggtggcg
     3361 gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg cccttctgcc
     3421 gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc ttcagtagaa
     3481 aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa atcgcttttc
     3541 aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag atcggtaacc
     3601 tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag cgggatttta
     3661 ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat ggcatcaatc
     3721 acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac gaaatcatcc
     3781 accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat gacttccgct
     3841 tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt ggtgacgcac
     3901 acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag cgcttccgcc
     3961 gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc aaacagttgc
     4021 aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca tgcatcacta
     4081 attaccacag acatagcacc tcagaattaa aaagggcgag gttatccctc accctaaacg
     4141 cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat attttggatt
     4201 tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac aacgcgcact
     4261 tagtcaataa tcaggatgtg aggttacccc tcccctgtca tatccgtaaa aacggcatac
     4321 agaatatcac atcagccgct aaagacgtta cctgcgcccg gagccgtttt cagcacccaa
     4381 acccgtccat agtggttgta ccaacctgcg cggtgtccgg cttccggccc gatcccttga
     4441 tagatatcga agtgttggcc tttgattgcg cccccgacat ccaacgccac catcagacgc
     4501 aattcgtact gaccgttaaa tttgccgtta ttatccagca gcggcacttc tgccagcaag
     4561 gtagtacctg gcggaataat tgaacgatcg gaggcaacag aagcacggcc aaccagcggc
     4621 accgcacttg cccctttcac cggtgcaaaa gattgcggtt taaagaagac gaaagacggg
     4681 ttctgttcca gcagctcgcg aacctcggct tcactgtgtg tttcgcccca atgacgaatc
     4741 gcctgcatcg acatatcttc ttttttcact tcgccacggt cgatcagcac cttaccaatg
     4801 ctgcgatagg catgaccgtt tttccctgca tagctgaaaa agttaagcgg actgccatca
     4861 ccaaagtcga tatacccact accctgcaca tccataatga agttatccat cagagagttg
     4921 ctgtaagcga ggatgtattt atcgcccaat gccccggcgt agatctccgc acgagacggc
     4981 agacgaccac gttttggcgg catacggtaa ataggatact ggaactcgcc ctggcgggta
     5041 tggcgcgcct gaattaccgg cgtgtaatag cctgtaaact gcacgttacc atagttgtcg
     5101 gcaccttcca tctgccaggc atcaatgccg aactggcgca tattgcgggt atcaccgcct
     5161 gcgcgcagcc actcttgcac cgcgttataa acattactct ggttgccata cagacgcggt
     5221 gacgaattac ggatatggtt aatttgctcg gcaaaatcac cggcgttaat cggcgcgcca
     5281 acggcatctg gctggttcac cagagagaaa ggctgggtaa atttcacgtc tttatattgc
     5341 tgtccgcgat cggttggttt ggaagagcag gcggcaagca ttgccacaac cgtgcccata
     5401 agaaggtact ttacccaacg tcctttcatt gttctcttct tcaggttaaa aaataaggcg
     5461 caacgaagat aacaaaccgc cgcgtttaat gaaatgagca gcctctccct gacgcaaatt
     5521 ttgcacaaaa aataggcttt agtggtttgt ttttgttcaa aatcatgcta aatccgtgat
     5581 cggggtaaaa aaaaggttgc atgaaaacgc gagcggagta tagtgcgcat ccacggacgc
     5641 ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg gttcaaatcc
     5701 ggcccccgca accaatcaaa tttgatgaag taaaagcagt acggtgacgc ggggtggagc
     5761 agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg gttcaaatcc ggcccccgca
     5821 accaattatt gaacacccta acgggtgttt ttttgtttct gatctcccat aaaaaaagcg
     5881 ccattcagcg cctttttatc atccccttct cgccagcgtt gccccatcgg caaaatacgc
     5941 tttaatcccc gcaagaatag actccgcaac ttcctgctgg aaagtcgccg ttttcagttt
     6001 acgctcttcc tcaacgttac tgataaacgc cgtttcgacc agaatggagg gaatatctgg
     6061 tgcctttagt acggcaaacc cggcctgttc aacttgattt ttatgcagct tgttgatttt
     6121 tccgagctta ttcagtaccg ctttaccaaa cttaaggctg tcagcaatag tcagcgactg
     6181 caccatatcg aacatggtgt ggtcgacata gcggtcaccg cttttgctca cgccaccaat
     6241 caagtccgag gcgttctggg tttgtgccag atattttgcc gcagtactgg ttgcgccttt
     6301 ggttgagagc gcaaacaccg aggaaccgct cggctgacga ctggtaaagg cgtcggcatg
     6361 gatagagaca aacaggtcag cacgctgttt ctgggctttt gctacgcgca cttgcaacgg
     6421 aatgaagatg tcttcattgc gcgtcatgta caccttcata ttgccctctt cctcgatcag
     6481 agagcgcaaa cggcgagcta tttgcaatac cacatctttt tcgcgcgttt tgtatttccc
     6541 caccgcaccg gagtcttcgc caccgtggcc agggtcaagc ataatgacaa tcggacgatc
     6601 gcgccctgct ttacccggtt gtggaccact ttgcgctggc ggcacctgct tttcgaggtc
     6661 gcctttgttg taatcctcca gcagcgccag cagcgggtcc tgcatatcct gtgcattggc
     6721 cggatagagg tccatcacca gacgctcttt aaacccggcg accggcgcaa gggcaaacag
     6781 ctgcggtttt acgttttgct ttaattcaaa aaccatgcgt acggtttgcg ggtcaaattg
     6841 cccgacgcgc gccgacttga tgaacgggtc gtctgcacga atttgtgccg ccatcccctt
     6901 gagtaccgag ttcaggttta catcttcgat atccaccacc acgcgttcag ggttactcaa
     6961 cgcgaactgc ttatatttca gctgacgatt tgattctacc gtcacgcggg tgtagctgga
     7021 cgcaggccag acgcgcaccg ccacgacctg gctaaccgca gccaggctga cctgacttac
     7081 gctcaatagc cacatagcgc ccgcgccttg cagtaaacga cgacggctga ttgcagtgtt
     7141 ggatcctgac atgcctctcc cgagcaaaag aaatctaagc tgtgtaacaa gtaaacgact
     7201 aatttgaccg gaaactttag cgaaagacgc ataatctgtc atctaataaa cggtaaacat
     7261 tctttttata ttcacggcat tactgataaa aaagtcgctc tcgcataaaa tttacacttg
     7321 caccctgcga aaaaacagaa taaaaataca ataatttcaa ataatcatgc aaaggggtgt
     7381 gccgtggtaa aggaacgtaa aaccgagctg gtcgagggat tccgccattc ggttccctat
     7441 atcaataccc accggggaaa aacgtttgtc atcatgctcg gcggtgaagc cattgagcat
     7501 gagaatttct ccagtatcgt taatgatatc gggttgttgc acagcctcgg catccgtctg
     7561 gtggtggtct atggcgcacg tccgcagatc gacgcaaatc tggctgcgca tcaccacgaa
     7621 ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa cactggaact ggtgaagcag
     7681 gctgcgggaa cattgcaact ggatattact gctcgcctgt caatgagtct caataacacg
     7741 ccgctgcagg gcgcgcatat caacgtcgtt agtggcaatt ttattattgc ccagccgctg
     7801 ggcgtcgatg acggcgtgga ttactgccat agcgggcgta tccggcggat tgatgaagac
     7861 gcgctccatc gtcaactgga aagcggtgca atagtgctaa tggggccggt cgctgtttca
     7921 gtcactggcg agagctttaa tctgacctcg gaagagattg ccactcaact agccatcaaa
     7981 ctgaaagctg aaaagatgat tggtttttgc tcttcccagg gcgttactaa tgacgacggt
     8041 gatattgtct ccgaactttt ccctaacgaa gcgcaagcac gggtagaagc ccaggaagag
     8101 aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg gcgcagtgaa agcctgccgc
     8161 agcggcgtgc gccgctgtca tttaatcagt tatcaggaag atggcgcgct gttgcaagag
     8221 ttgttctcac gcgacggtat cggtacgcag attgtgatgg aaagcgccga gcagattcgt
     8281 cgcgcaacaa tcaacgatat tggcggtatt ctggagttga ttcgcccgct ggagcagcaa
     8341 ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg aaatcgacaa attcaccatt
     8401 attcagcgcg ataacacgac tattgcctgc gccgcgctct atccattccc ggaagagaag
     8461 attggggaaa tggcctgtgt ggcagttcac ccggattacc gcagttcatc acggggcgag
     8521 gttctgctgg aacgcattgc cgctcaggcg aagcagagcg gcttaagcaa attgtttgtg
     8581 ctgaccacgc gcagtattca ctggttccag gaacgtggat ttaccccggt ggatattgat
     8641 ttactgcccg agagcaaaaa gcagttgtac aactaccagc gtaaatccaa agtgttgatg
     8701 gcggatttag ggtaagcgat gaaaatcacc ggatgcggca ggcgtaacac tcgtgcgtcg
     8761 catccggcaa ttacgtttat tcccgcgaac taaacaacgc cgccagacca ctgcgccgct
     8821 cagtacgagt ggcgattgcc gcacttaata tacgctcatc ggcatacagc gacagacgac
     8881 gacgcgcgcg ggtcaccgcg gtgtatacca gctctcgcgt tactaccggc gtgcgttggc
     8941 tcggtaaaat caacgccgca tggtcgaact ccgatccctg cgatttatgt accgtcatcg
     9001 cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac agacttaata ttgccgtccg
     9061 gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag cgcaataccg atatcgccat
     9121 taaacaaccc aagcgcgctg tcattacggg caatcatcac cggtcggcct tcgtaccaac
     9181 gagagtgcgg atgacgatga attttgcgct tctgttgcat aaactgttca attcgctcat
     9241 tcagtccagc cacgccaaac ggcccttccc gcagggcgca caaaagctgg tactcattga
     9301 acgcctgaat gattaaatcc ggctcggcac gcgcttgcag cagatccaga taacgtccgt
     9361 aacccgcaag agcttcctca agcatcgcaa tataatcttc gccgctctgt aaaagtcgtt
     9421 tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac tgccgtttta tcaccacggt
     9481 tgatcgccgc agctaactga ccaatgccag aatcgctgcc gaaacgatag cttttttgca
     9541 gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc ggttcctgcc ggaacgtgag
     9601 tccccgtcag gcggcttagc tgcctggcac gctcggcggt aaagcccgcg ttggcataag
     9661 cgcagatatc gcccagcaca gccccagcct caaccgaggc cagttgatca cgatcgccga
     9721 gaaagatcac tcgcgcatga tctggcaagg cgtcgatcag tctcgacatc ataggcagat
     9781 cgatcattga cgcttcatct accaccagca catcaagatg cagcgggtta ccggcatgat
     9841 gacgtaaacg ctggctaccc ggctgcgcgc ccagcaatcg gtgcaaagtg ctggcatctt
     9901 ccggaatgcg tttcttttgt tcatcggtca gcggtaactg tcgcaaagcc ttgccgagag
     9961 attcggttaa gcgcgcggca gctttacccg ttggtgcagc cagacggata cggcagcgtt
    10021 cgccgtcggc catttgaatt aacgctgcca gcaacttcgc cacggtggtc gttttaccgg
    10081 tgcctgggcc gccggaaatc accgagatcc gccgcgtcag cgccactgcc gctgcaactt
    10141 tttgccagtt aatttcatcg cttactggaa aaagtttgtc cagggtttgc gccagtagag
    10201 cttcatcaac ctcaatggta tgattcacct cattgaagaa gcgcgctacc gtccgctcgt
    10261 tacaccacat gcgattcaaa taaagacgat cgccacagag gatcatcggc gtgggttcat
    10321 ctcccctgct gaccgcttga gaagccagca agcattcttc ccaattttgt agctcaccga
    10381 tttcactgac acaggtcgcc aacagcggat tcgacgcctc gttattttcc agtcgtgaaa
    10441 gcggcaaaca gacgtgtccc tctccggcat catgacttaa cagtgccgcc gcgagggtga
    10501 cggcaggatg ttcatctccc gccacggtca gggcaaattg cacatccagc gggcgtagct
    10561 gtttgtgctc cacagcttcc agtaattgct tttgcaattt cattacgcct cctccagggt
    10621 cataccggca aacatctcat ccatcagggc aatcaacccg gcgttgggtc gggttgtgta
    10681 aatcccttgt tgcggatgtt ctttatcaac gccacgcagg aacagataga taacgccgcc
    10741 aaagtgaaga tcatagtcgt aatcagcaat gcgatggcgc agataacgat gcagcgccag
    10801 ggtataaagc tgatattgca gatcatagcg gtgtgcctgc atcgctgctg ccatagcctg
    10861 ttgggtgtaa gccgaactgt cttcacccag ccagttggat ttatagtcga gcaggtaata
    10921 acgcccttcg tggcggaata ccaggtcgat aaagcctttt aacatgccgc gtacctgcat
    10981 gaactccagc ggcgggcagc cagcggatag cgggtcaaac tggcgaatta gtgcatcaag
    11041 ctggctggcg atgagcggtt cgctaatcgg caggtaaaac tccatctcca cctgtttatc
    11101 gcggtcggaa agctgactca ggctaacacc cgtttcattg agaggtgcct ggaggacagc
    11161 cgtgatccac tcggtcaaca ccggttccca ctgcgattca aagccgccga gttccagttt
    11221 ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg aggtcttcaa acaaactgtg
    11281 caagaatgtc cccggtgacg caccgcgcgg gaactgatgc ggtgttaacg tcggttcttc
    11341 aacgacgctg accacgcctg cggcatcaac atccagccgt ggcatcagat cctgagcgat
    11401 accgtgacca cgctgctgca agccggagta gctggtgaca cgccagttat cgccgggcaa
    11461 tcgttgtagt gtcctcgcat tcagttctgc agttaaagca tcattaacct gccagggctg
    11521 gttatcacca gtttgtgccg tttgccaggc aatatcatca tcgcataacg cttcaataca
    11581 ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc agcaaacgcc cgagcgcact
    11641 ttggtggacg tcggtgtcac cttttttatc gccacgacgg cgcaccagcg gtgcaacgcc
    11701 gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa agcaaacgca gatcttccgc
    11761 cagacgttcg acctccgcga ggtcgacgct ttctggcgca gcattaagat ccaaaactgc
    11821 ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg acgcggaaat tggtaataaa
    11881 tggcaaccag accagcggat attccagccc tttcgattta tggatcgtga caatctgcac
    11941 cagatgttta tcactttcga ggcgcatttg ttggctggag gcattactgt ctggctcgag
    12001 gatatgttgc gataaccagc gtaccagcgc atgttcactt tccagctgcg ttccggcttc
    12061 ttgaagcagt tcgctgatat gcaagatatc ggtaagacgc cgctcaccgc ctgccgttgc
    12121 cagcaagttt tctgcaatgt tacgcgccga catcagcgcc cgcagcatcg gcataacgcc
    12181 acgtttgcgc cagatttgcc gataaccatc gaactcttcg actaccgcat cccacgcatg
    12241 ttcgtcatta ttcagcgttt caatatccag cgcattcagc cccatcattg acgttgccag
    12301 cgcactgcgc agggtgttct cacgttcggg cgtcatcacc gcctgcaaca accaaagcat
    12361 ttcctgcgct tccagagttt caaaaacact gtcgcggttc gaaaggtaaa cggaagggat
    12421 ttccagcaac gttaaggcat cgcgcacctg ggcggcctcc tggcggctgc gcaccagcac
    12481 gctgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg ttcatcagca acgcttcgcc
    12541 ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca catacctgcg ccatggtact
    12601 ttgatagtcg ccaacgccgc agctttcgcc ttccatcagc cacattttca tcgcaggctg
    12661 cgtttcacct ttaaatacaa aacgtaacgc ctgatttttc ccggctgatt tcactggaat
    12721 aaacggtatt tcgcgaaaca tgaacgtgtc atcagtctgg ctgaaaagct tgttcacact
    12781 gttcaccatt ccaggtgcgg agcgccagtt ggtgtctaaa gtgtagtggg catgaacttc
    12841 actacgcgcc ttcatataag tgaagatatc cgcaccccgg aatgcatata tggcctgctt
    12901 cgggtcgcca atcagcaaca atgcggtttc cggctgatgg tgccagatac ggcgaaaaat
    12961 tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc attgccaccg ggaatcgcgt
    13021 acggatcgcc gctgccaaca cctcgccgct ttcgctacgc agcgcggaat cgagccgact
    13081 taacatgtca tcaaaaccca attcgccacg gcggcgtttt tcacgcgcta ctgtttcgcg
    13141 gatctcagcc aatgcgcggg tgatcaacag atcgcggatc gacaatggtt ctgcaagcag
    13201 ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc cccccggcct tcgtgcgatc
    13261 ttctaagaaa cgctgggaga atttttccag cgactccggc aactgataac tgtttgtctc
    13321 ttcttctgcc caggcgctga tcttttcgat ccatttagcc tgattgctac ggttaaactt
    13381 gcgtcgatca ataccagaag attcgatcag cgcatccagt tcacccactg cgtcgcgcca
    13441 ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg tggcgggaag ccagcgtttc
    13501 atcatcgggc ggcggtgctt tgataaccgg cggttcgcct tgcagataac gattaatatc
    13561 gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacg acctgggcta tttcacgcgg
    13621 cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc tggtagcgta gcagagactc
    13681 atcttcaatc agctgctgct caaacagcat gccggattca aaggcattca ggttgagcat
    13741 gcgctggcaa aagccgtgaa tagtaaagac tgccgcttca tccatctgcc gttcggctaa
    13801 caacaaccac tgcgcggctt gcgctttatc gtcgatctct tccagcaggc gttcgtacag
    13861 tggattgtcg gtggtttcac gcagacaggc gatgcgcaac tcgtggatat tgctacggat
    13921 acgaccgcgc aattctgccg tggcagcctc ggtaaaggtg accaccagca gttcttcaac
    13981 gatcagcggg cggggaaagg cggcggaacc gcctagtcca agtaacaggc gcaaatagag
    14041 cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct tcaatcaggc gctcgccctg
    14101 taagggcaag cgcaaaggat ctagtgtctc ggcgacatca ctcattcttt tcactcatca
    14161 ggggcattgt ttgctgcaac gcgctgacgt tctcccacac tttccagcct tcaggatgca
    14221 catattcggc tttcccgttc tggctgccgg aaatctgcga cagaatagcc atgccttgcg
    14281 gctcgaccac cgcctgatgg aagaaatcag caagtttttg cggcgtcagc agttttatct
    14341 gggccacgat tttatcacgc gaatcgaagc gcatattgcc gcgatcgaaa tctttactta
    14401 acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag catctgggta attaccgcct
    14461 gctggatttg cgcaaactca tctggcttca tcgctcgcaa ttttgcctct gcggttggga
    14521 aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt atcattgctt tgcaaaagga
    14581 agcccatgcc ccactgacgc cccacgctca ttggaaacgc aaacacggca tagcccaatt
    14641 gttcttcggt acgcaactga ttgtagaacc acggctgtac gatctgcccc aacagagagc
    14701 tataggctga gctggtgtat tcatcgtagc cagtcggtac aaataccgct gccagtgcgg
    14761 agtcggtgct gttaccggct ttttcaaaga tgacggattg ttttttatcg accactacat
    14821 ctttgtttcg acaccactct gaaccatcag cgcccaactg tttttgcaca tcgcgtgcca
    14881 gcgttgttgc ctgggcctcg gtcatgttgc cgataaccat aaactctggt cgagcccctg
    14941 attttaaggc gtcgcgatag gccagcacct ctttcaacgt aatggagggc aaaattttac
    15001 gccgttcatc tcgcgagaag tacggcactt gcgagagcat ctgcgcgggc ataatcgcct
    15061 gctcaaacgc tttacccttt tctgcggaat ccatcatctg gttataccag gacttcgcct
    15121 gctcaagctg ctcttccgta gcggtatagc taaagtaccc ctcgagcaat gcctggaaca
    15181 gctgcggcag acgctgggtg taaccattag cattaatcat aaggccgttg ttagcgttgg
    15241 tggaaaaact tatgccacca accgacgcct ggttgcttaa ctgatcaagc gccagccctg
    15301 cgagataatc attgagcgca aacatcacct gattgcgggc gctgtccatg gctttcggat
    15361 tacgcaaaat caggctgaca tcagctttgg gctcgctggc aaaataacgg cttggcgcat
    15421 acaccacgcg cagattcgac tcatcaacaa tcagctctgg atggtcgtat ttcttctctg
    15481 acttaatcag cgagaaatca tcaggaatat aagggttaag ctctggcaaa gagagcgcaa
    15541 tgtcggcggc ttttttctgc cagtcggcga aagtttgtgc gctgatttta tcgacctgat
    15601 acggcgcatc gacaaagtaa gccgttttgt tgtgcggctc tttcgggctg atataccaga
    15661 tacgcgcatt ctgcggcgtc atcatcgcca gacgttcctt tactgcttta gcatcgtacc
    15721 gatcggcaat attgactgca tccagcgtat gctcaacagg aacgcgaatc atggtatctg
    15781 ccagccattc gacgtaatcc atatcacggg tgatcgacgg ataacggaag tcgatatcca
    15841 gcacattcgc cagttcatcg aagtattgtt tatcaatgcc tttttcacgt aacagattga
    15901 gatagctaaa aattgccgcc acaacctgat cgcgattagc caggccttta tcagttaaag
    15961 acgcagagat cgctaatacg ccgctgttgc cgttgacgat aggatcggag ttggcgctaa
    16021 tgccctcaac taatccctgc ttttgcagcc agtcagaaag tgtacctggg ctgcgattgc
    16081 caatcagata ggtaatcaat tcatcggttt tactacggaa cttcgctgag ttgttatcga
    16141 tgcgaaactc aacgcgcaac actttacgcg gcagcgcagg gacgtaatga atgataatgc
    16201 ccttttgcgc gtcggtgact accggcacgg tgatttccgg ttttttgctc tctttgttcg
    16261 gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc cggcagcggt ttattactgt
    16321 aaataaccgc cttcatcaaa ttggcggaat agtacttctc gtggaaatct ttcagcgcct
    16381 gctgcaccgg attaccaggt ttgtcgctta aagtttcgag gttaccacca gaaaactttg
    16441 aaccggggtg tgccgggtta atggtttctg cgctgacctg tgccatgcgc atcccgtcac
    16501 gcgtacgcgc catggttaat tcagcgttca ctgcattacg ctcacgttcg gcgtatttct
    16561 tgtcgagcaa gggttctgca atagcatcgg ccaggcggtc taccgcaccg ggcaaggcgt
    16621 cgttctcaac ttccagatag aaagccgtgc gatacggcgc agtgctggca ttgtgactac
    16681 cgccgtgcat tttgagatat tcggccagac tgtcagcctg cgggtacttt ttcgacccca
    16741 tcagactcat atgttcaagg taatgtgcca gcccctggta cgcctcggga tcttccagcg
    16801 acccaacggg cactaccagc gccgagagcg atttaactgc ctgcggatca gaaaccagca
    16861 agaccaccat accgttatcc agacgtatag cctgatactg gcggttatct ttatcacttt
    16921 tacggatggt ttcctgaatc ggctgccatc ccgtttctgc ctgacttaat ggtgcccaga
    16981 gggcaacaaa caacaataat gctttgaacc aggtgctgcg gggcattcac ggacctcata
    17041 atcaacttta ttttctggac agattcaaca ggttaacgca aatcatctgc cagaatttaa
    17101 tcttgtgctg cacgagccag cctatgttta tataaccatc agtccgtgac tggtgcgcat
    17161 cataaagtaa gcagatagat tgcgcaattt ttatacaaca ctcatgactg attaaagcga
    17221 aacagcggta acaggaaacg ttgcgactgt tcaacgatgg cctccattgt ctctggtgtt
    17281 aattgccgcc agagccgttg ataccagata tcatcacctt cgccacgcac catcatgttg
    17341 ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca acgtggaatc gtcatccagc
    17401 atggcatcgt tttgcgcgtc ataacaggtt tttagccacg cgccgccact ttcaggtaac
    17461 accagcaatg gcgcggacat tccttcacga tacccctcaa tcagttgtga gaggtaatgc
    17521 aaagcctgtt cggctgcaag cggcggaaaa cgccactcac cgtctttgcg tagaaaaagg
    17581 cgactttcgc cattaccacc gctggcacag tagacaaggt gttccagcca aagttgcatt
    17641 ccctgcgcca cacttaataa agagggacgc cagcgcaaca ggccatccgg ctgcacctgc
    17701 ggcaaccagc cagttatctg cacaccgttg caggcgagat caatttccat actctgcccc
    17761 ggctggcgac aggcaatgac tctgtcggca agctgctgca tctcctggca ctgtgtttcc
    17821 cagaaaattt caccaaaagc gccatacggt aaatcccctg ccgcccggaa gcggcggaac
    17881 aagcgttcgg catcatcctg ctcaaccagt gcattcaata actgctgatt gatttgataa
    17941 cggctaagtc cttccagaat aaatggctcg gtgtctggga tttcgctgtc ttcagtatgg
    18001 aagttcacct gcaaacgcat ctggaagaac gcccgcaccg gatgtgccca gaatcgttgt
    18061 agcgtttcca gcggcacggt ttccggtaag gtaaacggca gcggctgaac aaattcagaa
    18121 tgtgctttac cagcctggct ggccgcaggt agccattcac gagcatagct ttgtcgttcg
    18181 cctggctggt agttttgcgg atcaaacggc atccgggtat ggaggcaagt aagatgcgct
    18241 tttacccttg cctcgctttc atcacagttg agcgcttcat cgcccggtag ataatgactt
    18301 tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg ggaaacgttc actgttatcc
    18361 tgaatggaac gcccgatata gctgatatag agtttttgct gcgcggaaat taacgcttcc
    18421 aggaacaggt agcggtcgtc atcgcgacgg ctacggtcgc cacgcttcgg tttctggctc
    18481 atcaggtcaa agcccaatgg cgcaagctga cgtggataaa cgccgtcgtt cattcccagc
    18541 aggcaaacca ctttgaacgg aattgaacgc attggcatca gagtacaaat gttaaccggt
    18601 ccggcgagaa aacgctggct gatacgttct tgatccagac gctgtgccag ttcatcacgc
    18661 aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta aaccttcggc gataatcgcc
    18721 tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt ccgcatccgg caggaagaag
    18781 gcgttgagca tatcgcgaca aaccggcaac cactcttcta gcggacgctc ctgtgccagc
    18841 ccgcgacgcc agatgtttag ctgcattagc agtgaagcca gatgccccac cagttctgca
    18901 attaagccgc tcgattcatc ataaggtaga accgattgcc actcgccctg cgcgctctcc
    18961 atcgcgtagc ccagcaacat acgcgtcagg ccaaatcgcc aggtgtgttg tccggtggcg
    19021 gggagttcca gctcgcgaac gttgtcgtca tctatccccc aacgaatgcc ggattcgttg
    19081 acccactggc gtaaataacg cagcccttct tcggtgatgt caaaccgcgc cgccagcacc
    19141 ggcacatcca gcagcgccag cacatcctct gacacaaagc gactgtcagg cagtgataac
    19201 aggctgataa acgcttccag taccggatgt gactgccgcg cacgacggtc ggaaatggcg
    19261 taaggcaggt aacgatccgc aggtgcgcta ccaaacactg cctgaataaa cggactgtag
    19321 ctgtcgatat cagccaccat cacgatgatg tcgcgcggag taagtgtcgg ggcttcctcc
    19381 agcatcgcca gcaagcgatc gtgtaaaact tcaacttcac gctgcgggct atggcaaacg
    19441 tggaatgtga tactgctatc cagtggatca agcgggcgtt tgttatcgct acgggaaaac
    19501 tcttcgatgt tcacacccgc aacggcgcgg ttttccagtt ccagaatgtc agactgaatg
    19561 ttatgcagca ggttatctgg cgtcacatcg acaaaagcgt ccagctcctg gctgctctcc
    19621 aggtcagaaa ggagataaat gtagtcgcgc ccaagcttcc cccatgaggc cagcagcgag
    19681 ttgccgacat cctgttcacc atcgctgtta aagagctgtc cggcattttc gctatcgcga
    19741 aatagcggca attcgcgatc ttcaaaactg tgtcgacgct gacgagtcag cagtttcgcc
    19801 agataagcag gatctttaat atcgccccag taataacggc aggggttggt aaacaggaga
    19861 tggatttcaa tatgtttacc cagcgcctgg agcgcctgga gataaacagg cggtaacgcg
    19921 gaaataccgc atataaagac gcgcgaaggt aaccccggcg ggcaggtcgt cgcggactcc
    19981 agcgtttcga taaagcgctg atagagattc gcgcggtgcc agcgcggttg tccgagttca
    20041 tgggtatatt ccaccagcgc cttccacaac ggggcttgcc aggcctgtgc ttctcccagc
    20101 ccttcaacca aatgtcctgt ttcccactgt gccagccagt ccggacgata gaccagatac
    20161 tggtcaaaca ggtccgccgc ttttgaggaa agctggaaca gttttcgctt gtcgctatcg
    20221 tcagtcagat aatgccgcaa cagggtaaag tcttcgcgct ccagcaattg cggcagcaga
    20281 gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc tctctttggg gatttccggt
    20341 aacacccgga cgaacatatc ccagataaag ctcgctggca gcggaaaatc aatgtttgcc
    20401 gcaataccaa acttttgcga cagggtcatt tgcagccact gcgccatacc ggtactttgc
    20461 accagaatca tctctggttc gaaaggatcg tccagccgtt cgcgttcgac aataaactcc
    20521 atcaacgctt ccagcacgtc cagacgattg gaatggtaga cccttaacat agcggctcct
    20581 gactactgac aattcgggca atgcaggcgg gtcatctcgc cttctctgcc cccgggtgaa
    20641 actagcgtaa cgctgatgct gacacatccc gcctgcgatg tctgcattcg gttgacctgc
    20701 cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct gccaaccatg ccgccagagc
    20761 ttttggtact ggtttctgct ggcgagactg ttcattgatg ttcgctggat acccgataac
    20821 gcagtgacaa tcatcaccat cagcaccatc agcaccatca gcaccatcag caccatcgca
    20881 accattacct ccggcaggct aaagccttgt tgattcttca gggaagctga cataatgccc
    20941 cctctttcag cggacaaaaa tcgctccagc cgcgtggcga aaaaacaatt tttccatcaa
    21001 tgacttcgcc tgttcgccac aacgaaacgc cttcataacc ggcaatcaat aaggcttcat
    21061 tatctgccag taaacgcaaa caaacctggg catcggttcc agcgtactgc gagcactgaa
    21121 ctgctggctg cgtctgccag gagtgcattt ttccccacgc cagcgccgac tgaacgatgg
    21181 cctggcggcg caatgactga ctttccatgc tcacgcgaga ggcaaaactg cgatcctgct
    21241 gactcattcc ttgcaatagc aagctaccca aaaccagcag catcaggacc agagccagtg
    21301 acgaaacacc tttttcgcgg ttcacaggtt gaatcctgtc acgctatagc tggcatccac
    21361 cacggtttgc ggttcagact tactggcagc acgcatatta accgtcaaca ccggcgagaa
    21421 gccgctgaca tcctgacgta cgacctgaaa agtgtcgata atgatggcat ccggattagt
    21481 gactttatcc cagcccttac cttcacagga tgtcgcaccg cgtagcgttt ccagcacatg
    21541 ctccttcaga cgaaatccaa tctggtcgga ctcttttacc ggttcgcgat cccagatacc
    21601 gttactgttc gcatcccact gcacaatgat acagtcaccc tgtccgacaa tttccagccc
    21661 ttcgccggta cagctgccat gacaataacc cgccctctgg agatgcttcg cgacggtaaa
    21721 tacccgcagc cagatttcat cttccagtgc cagcttacga gtgctcgtta aactttcacg
    21781 ctgtaacgca ggcagaaagc gtgcagcccc cagcaacaat acgctactga tcgccatagc
    21841 aatcaacact tccagcagag aaaaaccttg ctcttttaca ggcatccttc tgtttctcct
    21901 tgctgataaa gccggagtct tccccacggc gaaaccacca gccaccactc gcccgttgag
    21961 tttttgaagc gaatatgcac ggcccatgcg gtattgcgca ggccaaagaa agcaagcgaa
    22021 ggtgtcaggt cgctcatttc gacttcgggc cagcgtggca caaagaccaa tggtgaactg
    22081 ccatgacagg tattggcccc agcagcggaa ctcacaaggc accataacgt cccctccctg
    22141 ataacgctga tactgtggtc gcggttatgc cagttggcat cttcacgtaa atagagcaaa
    22201 tagtcccgcg cctagctggc ggtttgccaa agccgttgcg actgctgcca gtattgccag
    22261 ccatagagtc cacttgcgct tagcatgacc aaaatcagca tcgcgaccag cgtttcaatc
    22321 agcgtataac cacgttgtgt tttcatgccg gcagtatgga gcgaggagaa aaaaagacga
    22381 gggccagttt ctatttcttc ggcgcatctt ccggactatt tacgccgttg caggacgttg
    22441 caaaatttcg ggaaggcgtc tcgaagaatt taacggaggg taaaaaaacc gacgcacact
    22501 ggcgtcggct ctggcaggat gtttcgtaat tagatagcca ccggcgcttt aacgcccgga
    22561 tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt agtcgaagat ggattcgggt
    22621 ttacgtttga taatcaactt cggcagcgga cgcggttcgc ggcttaattg cagatgagtt
    22681 tgatccatat ggttgctgta caggtgcgta tcgccaccgg tccagacaaa atcacccact
    22741 tccagatcgc actgctgcgc catcatatgc accagtaacg cgtagctggc aatgttgaac
    22801 ggcaggccga ggaagacgtc acaggagcgc tgataaagct ggcaagagag tttgccgtct
    22861 gccacataga actggaagaa tgcatggcac ggtgccagcg ccattttatc cagttcgcct
    22921 acgttccacg ctgaaacaat aatgcggcgc gaatccgggt cgtttttcag ctggttcagt
    22981 accgtagtaa tctggtcaat atgacgacca tctggcgttg gccaggcgcg ccactgttta
    23041 ccatacactg gcccgaggtc gccattttca tcggcccatt cgtcccagat ggtgacattg
    23101 ttttcgtgta gataagcaat gttagtatcg ccctgcagga accacagcag ttcgtggatg
    23161 atggaacgca ggtggcaacg tttggttgtc accagcggga atccatcctg caggttaaaa
    23221 cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt tttctgtgtg
    23281 ccttcgtcga gcactttttg catcagttct aaatactgtt tcatggttcc tcaggaaacg
    23341 tgttgctgtg ggctgcgacg atatgcccag accatcatga tcacacccgc gacaatcatc
    23401 gggatggaaa gaatttgccc catgctgatg tactgcaccc aggcaccggt aaactgcgcg
    23461 tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc cgtagccaat caggaacaag
    23521 cctgagacag ctcccattgg gcgcggttta cgaatataca ggttgaggat aataaacagc
    23581 accacacctt ccagcagcag ttcgtaaagc tgtgatgggt ggcgcggcag cacaccgtaa
    23641 gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca gcaaaatatc ttctgtacgg
    23701 gagcctggga acagcatggc aaacgggaag ttcgggtcaa cgcggcccca caattcaccg
    23761 ttaataaagt tgcccagacg cccggcacca agaccaaacg gaatgagtgg tgcgataaaa
    23821 tcagagacct ggaagaagga acgtttagta cggcgggcga agataatcat caccacgata
    23881 acgccaatca ggccgccgtg gaaagacatg ccgccgtccc agacacggaa cagatacagc
    23941 ggatcggcca taaactgcgg gaaattgtag aacagaacat aaccaatacg tcccccgagg
    24001 aagacgccga ggaagcccgc atagagtaag ttttcaactt catttttggt ccagccactg
    24061 cccggacgat tcgcccgacg tgttgccagc cacattgcaa aaatgaaccc caccagatac
    24121 atcaggccgt accagtgaag cgccacgggt cctattgaga aaatgaccgg atcaaactcc
    24181 ggaaaatgca gatagctact ggtcatctgt caccacaagt tcttgttatt tcgctgaaag
    24241 agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc gaataatagc
    24301 acaaggggac ctggctggtt gccggatacc gttaaaagat atgtatatga tccgcgctat
    24361 aaccctccgc gaatcagccc gcccatgccg cgacgctcca taaaggctgc aacctgatga
    24421 cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg caagattttc tgcttcggca
    24481 aaatcaatgc gccgcagcag gtattttacc cgcgctacag aacgtccgtt catagacaga
    24541 tggcgatacc caagcccaat gaggattgcc acgcacatgg gatcgcccgc catttcaccg
    24601 cacaaacgaa gatcgatacc atgtatttcc gcttcccggg cgatcatcgc cagagctcgt
    24661 aacattgcag gatgaagact gtcgtaaatg ttcgccaccc gggtattgtt gcgatcaacg
    24721 gccagaatgt attgagtcag atcgttggtg ccaacagaga tgaaatcgac cctctttgcc
    24781 agatgcggca gcataaatac cattgacggc acttccagca tgatgccgat acgtggtttg
    24841 ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg cacgttcaat caggcggcgt
    24901 gcttcgtcaa cttcatcgag gctggtgacc atcggcaaca gaatattcag gttgcccgta
    24961 gcggcattag cacgcagcat cgcccgcacc tggatcaaga agatctccgg ctgatcgagc
    25021 gtaatgcgaa tcccacgcca acccaggcat ggattctctt cgctgatcgg catgtaaggc
    25081 agctgcttat ctgctccgac atccagcgta cgcaaggtga cgggtttatc attgaacatt
    25141 tgcagcatcc cctgatactg cgccacctgt tcttcttccg acgggaaacc actttgcagc
    25201 atgaatggga tttcagtgcg ataaagaccg atgccatcaa tacagctgcc cagtttttct
    25261 tcatgttccg ggcttaaacc agcattgagc atgactttta tacgctcacc gctttttaac
    25321 tgcgcgggta aattgacgtc atcttccgcc agacggctaa gctcaatctc ttcactaatt
    25381 agccgctgat attcttgcag cagtaccggc tccggatcga ccagcaattc accgcgatag
    25441 ccatcaacga tcagcgtcct acgatgcagc accgaaggct gaatatccgc gcccatcacg
    25501 gtagggatcc ccagcgcacg taccatgatc gcagcatggg agttggctgc gccatctcgc
    25561 acgacaacac cgactaagcg atcctggggc agctcagcaa gagttgtcgc tgacagttca
    25621 tctgccacca gaatgaaacg ttccggccag gcgttcggcc cttgattagc gtcatcaaga
    25681 tgaaacagca atcgctgacc cagcgcacgt aaatcaccag cccgctcttt gagatagtta
    25741 tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga ccgtttttac cgcccactct
    25801 gccaccgagc ctttatcaac ctcggcaaac aattcgcgac gcagccgggt gtccgaaagc
    25861 aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt gtgctccggc ggcaaagcgt
    25921 ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg ccccggtcag tcgttcgcgt
    25981 tccagagccg gatccagcgt tgatgcctga tacacctgtt ccattaaagg taacgtggca
    26041 tcctgccagc cttcggcaat cgccacacca ggtgctgccg gtaatgcgcg gattcgcgtc
    26101 tggcgatatt gcccaaacag ggcagtcaac tgcgactgag aaagaatagc tgccatctgg
    26161 gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat actggcgcaa ctctcgttgc
    26221 tgtaccacca gtacaccaag caactggcga cgttgaataa ttggtacgcc taaaaacgcg
    26281 cggaaacgtt cttcttttac ggaggggatg tatttgaagc tggggtgctt ttgcgcatct
    26341 gcaaggttta tcggttccgc cagcctgcca accaggccga cgatcccttc atcaaacgcg
    26401 agcgttacag tgcgaccgcg tggttttttc agcccccggg tcgccatcag gtagtaacaa
    26461 cgtcgatcat gatcggccag gtagaccgaa cagacctcgg tatccatcgc aagacagatg
    26521 tcggtaacca gaatatttaa cgcctcattc aggcgtggtg cgctggctac cttttcgact
    26581 atttcgcgca ggcgagtgag cataattggc gtgacttaac ctcttttacg tcgataagca
    26641 gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca tcaccacact cgcgaactct
    26701 ttcattaccc tacggtagac atcacgttta aatgacacca cctgtctgac cggataccag
    26761 taacttaccc atcgccagcc gtcaaactct ggtgtactgc tggtttgcat attgatttct
    26821 gcatcgccgc ttaccagctg caagagaaac catttttgtt tttggccgat acaaaccggc
    26881 ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt acgcgttgaa
    26941 gcaaggattc gaacgtcttt gcggcttaat cctacttctt caaacaattc acggtacatc
    27001 gcctgctctg cggattctcc ggggttgatt ccgccttgcg gaaattgcca ggagtgctga
    27061 ccaaatcgcc gggcccacat tacctgcccc tggcgattac aaatcacgat acctacgttt
    27121 gggcggtagc catcgtcatc aatcaccgga ctacctcaaa ataaagcttt atatacgaat
    27181 gattgtttca tactccagga agacggtaaa ccactctctg cagggcatta cacactaata
    27241 acaattgaat aactcacagt tatgtgcaga gttataaaca gaggaagggg tggatagccg
    27301 tttttattca ccttttctgt ggatagagtt gtgaagaact acggaattac tacgggaaaa
    27361 cccggagcat tctgaataag cccgatatag acattttaaa atattcatta ttagtcaatg
    27421 cattacatgt aatttgagta atgaaaaaga gtaattcgtg acccaggtca cacctctcat
    27481 ttacgggttg gcgaaagatc aaccaatgcc gtgtttatcc acagaatgtg ccactaagtt
    27541 aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa atgaaaacag
    27601 tagtggaggt ttttcacagt tatcccagct ttctgtggat aacatggtgt aagatcctgt
    27661 ttattttcag tgaccagatt tggaaaacct attgcagtgt tgcgcaactc gattaccggc
    27721 agcctaaaaa gcaatataaa tcagagaatt gaacaacaca tgtggaaaaa gttacactgc
    27781 gaatattcgg cacataattg ctgtttgttt tttaaccaag gtatcatgac atgtcccaac
    27841 ctcgcccact gctctctcct cccgaaactg aagaacagtt gttagcgcaa gcacagcaac
    27901 tttctggtta tacattggga gaactggcgg cacttgacgg gctggttacg ccagagaatt
    27961 taaaacgcga taagggctgg attggcgtgt tactggagat ctggctaggt gccagcgcag
    28021 ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga acttaaaact atccctgtgg
    28081 atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt tgcccggtta acgggtaata
    28141 gcggggtgac ctgggaaacc agccatgtgc gccacaagct caaacgcgta ctgtggatac
    28201 cggttgaagg cgagcgcagc atcccgctgg cgcagcgtcg cgtaggatca ccgttgctgt
    28261 ggagcccgaa tgaagaggaa gaccgacagc tacgcgaaga ctgggaagaa ttaatggata
    28321 tgattgttct cggtcaggtt gagcggatca ccgctcgtca cggggaatat ttacagataa
    28381 gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat tggtgcccgg ggcgaacgga
    28441 ttctgacgct gccacgcggc ttttatttga agaagaattt caccagtgca ctactggccc
    28501 gtcattttct gatccagtag ccatcgcttt gacctgccgc tttccgggca tataattacc
    28561 gcttcatttt tttggcaggg ctttttagat gttatttgca tggataaccg atcctaacgc
    28621 ttggcttgcg ctcggtacgc tgacgctgct ggagatcgtt cttgggatcg acaatattat
    28681 tttcctttct ctggtggtgg caaagcttcc cacagcacaa cgtgctcatg cgcgccgtct
    28741 ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg gcatcaatct cttgggttac
    28801 gcgcctgaca aatccgcttt ttacaatatt cagtcaggaa atttctgccc gtgatttgat
    28861 tctgcttctg ggtggcttgt tccttatctg gaaagccagc aaggaaatcc acgaatccat
    28921 tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg ctatcgtgca
    28981 gattatgctg ctggatatta tcttcagcct cgactcggtg attaccgctg tgggtctgtc
    29041 agatcacctg tttattatga tggcagccgt ggtgattgcc gtaggcgtga tgatgttcgc
    29101 cgcgcgctcg attggtgatt ttgtcgaacg ccatccttcg gtaaaaatgc tggcgctctc
    29161 tttcctgatt ctggtgggct ttaccctgat tctggaaagt ttcgacatcc acgtaccgaa
    29221 aggttacatc tacttcgcga tgttcttctc tattgcggtt gaaagcctca acttgattcg
    29281 caacaaaaag aatccgctct gatactccgt acgctctcct gcgggagggc gtactcactc
    29341 cttttaccct cacagtttaa gattttcctg ctttcaagat atataacgtc ggtttataaa
    29401 cagactatta tcataggtag gccaaatgag gataaccgga tgaaaaaatg ggcagtaata
    29461 atttctgcag tcggactggc gtttgctgtt tccgggtgtt ccagtgatta cgtcatggcg
    29521 accaaagatg gccgtatgat tttgaccgat ggaaaacctg aaattgatga tgataccggg
    29581 ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga tgatgtttcg
    29641 caaattattg aacgttaaca aataaggtca gcatccggct ggccttaaga tttttctctt
    29701 tcccttttcc cttccctctg ccatttttat attccttatg tcgtgattat aaaaaggaaa
    29761 cggctatgca atatcaccgt ataccccaca gttcgctgga agtcagcacg ctggggcttg
    29821 gcacgatgac gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa ctcgactatg
    29881 ccgtcgctca gggcattaac cttatcgacg ttgccgaaat gtacccagta cctccgcgcc
    29941 ccgaaacgca agggttaacc gaaacctacg tcggcaactg gctggcgaaa catggcagcc
    30001 gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc gcgcaataat gacaagggca
    30061 tccgcccgga tcaggcgctg gatcggaaga atatccgcga agcgctgcat gacagcctca
    30121 agcgcctaca gactgattac ctcgatcttt atcaggtgca ctggccgcag cgcccgacca
    30181 actgcttcgg caaactcggt tatagctgga cggattctgc gcctgcggtt tcgctgctgg
    30241 atacgctgga cgcactggca gagtaccaac gcgcgggaaa aattcgttat atcggcgtgt
    30301 cgaacgaaac tgcatttggc gtaatgcgct acctgcatct ggcggacaaa cacgatctgc
    30361 cgcgtattgt caccattcag aacccttaca gtctgttaaa ccgcagtttt gaagtaggtc
    30421 tggcagaagt cagccagtat gaaggggtcg aactgctggc ctattcgtgc ctgggtttcg
    30481 gcacgctgac cgggaaatat ctcaatggtg caaaacccgc tggcgcacgt aatacgctct
    30541 ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg gcgtatgttg
    30601 atatcgccag acgtcatggc cttgatcctg ctcagatggc gctcgcgttt gtacgccgtc
    30661 aaccgtttgt tgccagcact ctgctgggcg caaccacgat ggatcagctg aaaactaaca
    30721 tcgaaagttt gcatctggag ttaagcgaag acgtattagc tgaaattgaa gcggtacatc
    30781 aggtttatac ttatcctgca ccataaagat taacgccggt taaaaccggc gttaaatatt
    30841 aatgacggcg ctgccagatc cacagcgccg ttattgccag cgcaaatagc gcaccgaagc
    30901 caatgccaat gggcacgacc gggatgccta ccattaccgc cagcgagtaa atgcccagca
    30961 tcaacaacat ggcgctgttt tcgccaaggt tttgtactgc aatcgcattc cccgccccga
    31021 cgcttttttt accccgctcc tgtagcaacg cattgagcgg aacgacaaaa aagcccccca
    31081 gcacgccaat cagcatcaac aaggcatagg ctggcagcag ctcgtgttgc agggagaaaa
    31141 tcggtaccac cacgccaatc aaaatcccgg ctggcataca gcgtgacacg gtttccagcg
    31201 taaccagctt cgctgccgca cctgcgccaa ccacgatacc aatcgctacc atcgcgttga
    31261 gataggtcgg cgtagcgtta tcggtaatgc ccagcgccac cggtacccac agcaccaaca
    31321 ggaaacgcag cgtgacaccc gctccccaga ataaactggt gcccaccagc gaaaaacgcg
    31381 tttcaccatt gcaccatagc gaggtgcagg cattcaggaa actgcgggtc atgttgatga
    31441 gattccagga ctgccccgga cgcgccgcca gtttgggaat gtagatattg gcaacgaccg
    31501 caccaccgta ggccagtgcg catgcggcca gagcgaccag gacatgccag tcagccagca
    31561 caccaccggc tacggaaccg agtaaaatcg ccgctatggc agaagcttcc attaaaccgt
    31621 tagcttttac taacttacta cccgtggtta attcgccgag aataccgtat ttcgccggtg
    31681 aataggctgc agcaccaaca cccaccagcg tatagccgag aaacggattg ataccaaagc
    31741 agatactggc tgcgcccagc agcttcaggc cgttggcaaa catcatcacc cggcctttgg
    31801 cgaagctatc cgccacctgc ccgacaaacg gcgccaaaag aatgtaagca cctacaaaca
    31861 ccatttgcag gatgggctgg ctccactccg gatagaactg cgctttcagt aacgccagag
    31921 tggcaaacag tagcgcgtta tcgccaaacg cagagagaaa ctgcgccacg ataaccgctt
    31981 tcatcccctt cgaccacaac gaagtgttag tgtgcactga ctcactcatc gtgttgttcc
    32041 gcttcgtcta cccagctttt caacgtgaca aagtcaggtt tgccgctgcc aagtaatggc
    32101 atctgtttca gatagcgaat atcgcgcggt acagcaagct ccggcacgcc gtgctcgcgg
    32161 gcatactgtt gcaacttatc gcgcgtcagt tcgttatctg tggtgaaaag caccagtgcc
    32221 tcgcctttgc tggcatcgct cttaatcgca gtggcatgga ctttatctgg cgaaacacca
    32281 agtgccagtt gttccaccat ttccagcgac accatttcgc ctgcaatttt ggcaaagcgt
    32341 tttgcgcggc cctgaatctg cacaaagccc tgctcgtcaa aacgcacaat atcgccagtg
    32401 tcataccagc cgcgctccat ttcgccgcga acattctcgg cggtgggcac ttccagtaca
    32461 cctggcttct ccacccgcag atagccgttc attatgttcg gccctttcag ttgcaggcgt
    32521 ccgccctctt cgataccagg gaccgacaac aggcgtgcat ccattcctgg tagaatacgc
    32581 cctaccgtac cgggtttcgc cgccatcggt acgttgatag aaacgacagg cgcgcattcg
    32641 gtcacaccgt agccttcaag gatgcgcagg ccaaatttat cctgccaaag ctgtttggta
    32701 ctttcttgta gtttttctgc gcctgccacc acatagcgta gacgatagaa gtcatacggg
    32761 ttggcgaaac gcgcgtagtg accgaggaaa gtcgaggtac cgaacaacac ggtgcaactg
    32821 cggtcataca ccaagtccgg cacaatgcgg taatgcagcg ggcttggata aaggaacact
    32881 tctgcacctg taagcagtgg cgtaaacagg cctaccgtca gcccaaagga gtgaaacagc
    32941 ggtaatgccg acataaagcg atcgttggtg gtgaagtcgg caatcgtttt aatctgctcg
    33001 acgttcgcca gaatgctttt atggctatgg acgacgcctt ttggatggcc ttcagaaccg
    33061 gaggtaaaga ggatcagcgc ctcttcttcc ggctgctgtt ttacctgcgc caggcgcggc
    33121 atcagcaaat gagcgaagat ccatactttg tcggcagtgg tgacatctgc ttttaaatct
    33181 tccagataga cccagcgcac ctgagtaagt tgctccggca gatgccagag tttgccttta
    33241 tcgagaaact ggcgggaagt gaagatggtt ttgatttcag ccgccgtaat agcactggtc
    33301 agccctttta ccccggcagt gtagttcatc attgcgggaa tgcggcgacg cgcgatggcc
    33361 ccaaaaatca ctgccgcact gatgcctgca ttgggcagca ttaagccgat gcgttcgcct
    33421 tcaacactgt atttttcaag gatgcgtcca acaaacagcg ttttcgtaag caatttgcga
    33481 taggagtctg gggtaaagtt gacgtcttcg acacatttct tcccggctcc gaagcggtac
    33541 attgcactca gtaaagattc gtacagagtt tcacgcgggc gcaccgccat acgcgcttcc
    33601 atcattattt gatgcagcat ttcgccagcg attttgcgac ggtcacgggc acgcggcgca
    33661 tccggcatcg ccacctgcgt tggaggcaaa atatgcagag taatttgcgg gaacaagcga
    33721 cgtttaacca gacctttcag gcggctgaag tgcgtaagtt ccgccccttc aatacgcaca
    33781 ggaataaccg ttgcaccaga cttcgccgcg acaaaacccg cgccatcgta gattttcatc
    33841 agcgagcctg tagtggtgat gcgtccttca gggaaaatca ccactggtcg gccctgttcc
    33901 accagacgta ccagatgttt aatagccata ggttgcgtcg ggtcgagagg aacaaagtcg
    33961 ataaatgatt tcagccaacg catataccac tgttggctta ttgaggtgta aacggcaaac
    34021 actggacgca caggtaaaaa cagtccaagc aaaatgccat caataaaaga gacgtgatta
    34081 ggcgtaatta gtacgcgctc gcccttcagt gcctgggtgt cacccgtaac gcgaacgcga
    34141 tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca ttccaactcc ctttgctctg
    34201 attcagtaaa agcgaatgga gggagattac acgagataaa gaacgcgagc gacagtaaat
    34261 taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg caagagacag ggtacgaaga
    34321 gcgtaccgaa taatctcacc aatcaatacc tctgggatct tgattgtggt ctgcacgacg
    34381 actcttcgcc agcgagaaaa cgcaaaggaa tgaagggaaa tgcaacgagg tgtgtaaatt
    34441 gtcggttact gttacagatt gatgaccggc aaaaaaaaac ctgcgcatct gcgcaggctg
    34501 gtgtaattca tgtgctcaac acgaagttga cttcacctat caatacctct gggatcacca
    34561 ctttagcaac ctgaagccaa acgccaccag cggtcaatcg caacagcgtt tcgcaaagtg
    34621 taaccaaagg tttgcaatct ctttttttgt cttacttatt tgcgtttggc tcacattccc
    34681 acgatgaaaa cacgccaccc cttgaaccaa cgggcgtttt ccgtaacact gaaacaatgt
    34741 aagcgtttac ccactaaggt attttcatgg cgaccataaa ggatgtagcc cgactggcag
    34801 gcgtttcagt cgccaccgtt tcccgcgtca ttaataattc acccaaagcc agcgaagctt
    34861 cccggctggc tgtgcatagt gcaatggagt ctcttagcta tcacccgaac gccaacgccc
    34921 gtgcgctggc gcagcagacc actgaaacgg tcggtctgat cgttggtgat gtttccgatc
    34981 cgtttttcgg tgcaatggtg aaagcggtcg aacaggtggc ttatcacacc ggtaatttct
    35041 tattgattgg caacggttac cacaacgaac aaaaagagcg tcaggctatt gagcaactga
    35101 tccgccatcg ctgtgctgcg ttggtcgtcc atgccaaaat gatcccagat gccgatttag
    35161 cctcattaat gaaacaaatg cccggtatgg tgctgatcaa ccgtatcctg cctggctttg
    35221 aaaaccgttg tattgctctt gacgatcgtt acggtgcctg gctggcaacg cgtcatttaa
    35281 ttcagcaagg tcatacccgc attggttatc tgtgctctaa ccactctatt tctgacgccg
    35341 aagatcgtct gcaagggtat tacgatgccc ttgctgaaag tggtattccg gccaatgatc
    35401 ggctggtgac atttggcgaa ccagacgaaa gcggcggcga acaggcaatg accgagcttt
    35461 tgggacgagg aagaaatttc actgcggtag cctgttataa cgattcaatg gcggcgggcg
    35521 cgatgggcgt tctcaatgat aatggtattg atgtaccggg tgagatttcg ttaattggct
    35581 ttgatgatgt gctggtgtca cgctatgtgc gtccgcgcct gaccaccgtg cgttacccaa
    35641 tcgtaacgat ggcgacccag gctgccgaac tggctttggc actggcggat aatcgccctc
    35701 tcccggaaat tactaatgta tttagtccga cgctggtacg tcgtcattca gtgtcaactc
    35761 cgtcgctgga ggcaagtcat catgcaacca gcgactaacc gcagttaaag caattccagc
    35821 gccagtaatt cttcgatggt ctggcgacgg cgaatcaacc gcgcctgacc attatcaaac
    35881 agaacttctg gtaacagcgg acggctattg tagttggatg acattgatgc gccatatgcc
    35941 cctgtatcat gcagtaccag ataatcacct gccttcactt ccggcaaggc gcgggtttca
    36001 acatttcccc cttcctgctg ggtaaagaca tcgcccgatt cacataacgg cccggcgacg
    36061 acggtttcca ccgttggcgc gtgttccaga gaacgaccat cagctgccag ggcactgata
    36121 tggtggtaac taccgtacat tgccgggcgc atcagatcgt tgaacccggc atcaaccagc
    36181 acaaagtggc ggctacccat ttgtttgacg ctccgcacct gagtaattaa tacgccagac
    36241 tgcgctacca ggaagcgacc cggttcaatt tccagtttca cagggtggcc caaatggcga
    36301 gcgatttgct cacgcgcggc attccacaga ccataataat gttcggtatc aaccgcctct
    36361 tcaccctgtt gataaggaat agaaagccca ccgcccgcag aaatagcctg taaatcctga
    36421 ccgaattcga taacctgacg caccatagca ccacacacct gctccagatg ggcataatca
    36481 acgccagaac caatgtgcat gtgaatgccg actagctgca gatgatgacg ttgtatcacg
    36541 tccagtgcgg cgggcagatc ggtgtaccag ataccgtgct tgctgttttc gccaccggta
    36601 ttggtttttt ggctatgtcc gtgaccaaac cccggattaa cgcgcagcca tacccgatgc
    36661 cctggcgaaa cctggcccag ttggtcgagc gtatcaacag aacccgcatt caccggaatt
    36721 tgcaattcac tgacgcgttc aagcgtcgcc tgatcgataa catctgccgt aaaaacaata
    36781 tcatcggggt gcgtttgcgg attgtaaccc gccgccaacg cacgctctat ttcgcctaac
    36841 gagacggaat ccactttcac gccctgctca cgcattaagc gcaaaatatg aatattggaa
    36901 caggctttct gtgcaaagcg caccacttca aactgtttca gcgctgcaat ctgccgacga
    36961 ataatttgcg catcgtagac ccacaccggg cagccaaatt cagcgggcaa acgcagcaga
    37021 ttttcggcgg tgagatcggt atcggtgctg aacagtgaat gtggcataac aaactccaga
    37081 tgagtgcttt tttatgatta cgccacatca taaaaagaat aaaaaatatc gatttatgtc
    37141 gagtctatgc aaaaatgata tggattaccg gattgcgaga gagcgctaat ggccgccgtt
    37201 aacttacgtc atattgaaat ttttcatgcg gtaatgaccg ccggaagcct gactgaggca
    37261 gcacacctgc tacacacctc acagccaacc gtcagccgcg aactggcgcg ctttgagaag
    37321 gtgatcgggc tgaaattgtt tgagcgcgta cgtggacgat tacatcctac cgtgcaagga
    37381 ctgcgtctgt ttgaagaagt gcaacgatcc tggtacggac tggatcgcat tgtcagcgcc
    37441 gcagaaagtc tgcgcgagtt tcgccaggga gaactgtcta ttgcctgcct gccggtcttt
    37501 tcgcaatctt ttttaccgca gctcctgcaa ccctttctgg cacgttatcc cgatgtcagc
    37561 ttaaatatcg tgccccagga atcaccgcta cttgaagagt ggctctcggc ccagcgtcat
    37621 gatttaggac tcactgagac actccatacg cctacgggaa cagaacgtac cgaattactc
    37681 tctttagatg aagtgtgtgt gttacctccg ggtcatccgc tggcggtaaa aaaggtatta
    37741 acgccggatg attttcaggg tgagaattac atcagccttt cccgtactga cagctatcgc
    37801 cagttgctgg atcagctatt tactgaacat caggttaaac gacgcatgat cgtagaaacc
    37861 cacagcgccg cgtcagtctg cgcaatggta cgggcggggg taggtatttc ggtggttaac
    37921 ccgctcaccg cactagatta tgcggcaagc ggtttagtgg tgcggcggtt cagtattgcg
    37981 gttccgttca ccgtcagcct gatccgcccc ctgcaccgcc cgtcatcagc gctggttcag
    38041 gcgtttagtg ggcatttaca agcggggtta ccgaaactgg tcacttctct tgacgctatt
    38101 ttgtcgtcag ctacgacagc ataaaagcga cagcatcctc ggcatggatc gccgcggtat
    38161 caaacacagg caaaacactg cgctcttctg gcaccagtaa accaatttct gtgcagccaa
    38221 aaatgacgcc ctgtgcgccc tgttctgcaa ggcgagcaat cacttgcgca taataagcgc
    38281 gtgacgcttc ggtaaattgc cccagacaca gttcttcaaa aataatctga ttaattttcg
    38341 cccgttcatc cgcttcagga ataagacaat tgatggaaaa ttgttccgtc agccgcccgc
    38401 gataaaaatc ctgttccatg gtgtaacgcg tacccagcag cgccacacga gtcattccgg
    38461 ccccggtaat tgcacgtccg gtggcatccg caatgtgtaa gaaaggcaga gtgcaacgtg
    38521 actcaatggc atccgccact ttatgcatcg tattggtaca taacacaata ccttctgcgc
    38581 ccgcccgctg taagccaagc gccgcctcag ccagaatgtc cccggtttta tcccattccc
    38641 cgcgacgctg gcactcttct atttcatgaa aatcgacgct atgtagcagc atttgcgcag
    38701 agtgaagccc accaagccgc tgtttaatgc cttcatttat cagacgatag taaggaatgg
    38761 tggattccca gctcattcct cccagcaaac caattgtttt cattcctctc tcctgatgtg
    38821 tgttaccgca acaaggctga ataaggaacg agacaaacgc ctcaacggcc aggtgcccaa
    38881 tctctattaa cgaaaaaagg gccggatgta cagcacatcc ggcccgtgaa atcagacgcc
    38941 gatatttctc aacttctcgc ctgccatcag tttgcgttcg atatgttcca gcgtgacatt
    39001 tttggtttcc ggaatgagcc agaaagtaat acccacaaac gcaatgttca gcgcagtgta
    39061 gagccagaac gtaccggcag cgccaatgct atcaagcagt gtcaggaagg ccgcgccgat
    39121 aatcatattc gacacccagt ttgtcgtcgt cgaacaggta ataccgaaat cgcggcattt
    39181 cagcggctga atttcagagc acaggatcca caccactggc gcggcgctca tcgcataacc
    39241 ggcaatacac atcatcgtca tgccaacaga gagccaggac aagccactgg aagccgtacc
    39301 gttatcaaac tgcatcaggc aatagcccag caccagagtg cctaacgcca tcacgctgaa
    39361 accaattttc agagccggtt tacgacctgc tttatctacc gtaaagaccg caatgaaggt
    39421 cgcgaacata aaggtcagtc ccacgaccag agtcgcaatc atctgttgtt ctgtggtcgt
    39481 aaagcccgcc attttgaaga tacgcggcgc gtagtacatg atgatgttca taccggtaaa
    39541 ctgctgcatc gcctgcaaca acataccgag aaacacagca cgacggacgt tacggttgat
    39601 cttaaacagt gcccaaccgc cctgttttaa cttcaggctt tcacgaattt cgttgagttc
    39661 ttctcgcgct ttttccgacg tatcgcgcag catacgcaac acttcttccg cctcaatatg
    39721 acgccccttt tccgccagcc agcgcgggct atttggcagg aagaccacca gaataatcag
    39781 cagaactgct ggtaaagcaa gaacccccaa cattgcgcgc cagttaccgc tataactgaa
    39841 cgctgtatcg gataaaaacg ccagcacgat gccgagtgtg accatcaact ggtacatact
    39901 gatcatctta ccgcgaacgt tttcacttgc catttcagaa agatacagag gagcggtgta
    39961 agacgcgatc ccgacagcaa tgcccagcac cacacgagcg gcgattaaca tctctacgct
    40021 ggtcgcaaaa gcggacccta tagaaccgag tacaaacagg atggcccccg ccatcaggct
    40081 gtatttacgc cccaggcgga acgacagcca accattaaac agcgcaccaa ttgctgcgcc
    40141 gagcatcatg ctactaacca cccattcctg caaacgactg gtcagcacaa agtgatcggt
    40201 aatgaacggc aacgctccgg cgattacgcc gatatcaaga ccaaataaca atcctgcgac
    40261 cgcagcagct accgaaacaa acatattcat acgccgcgta tcacgcaaag aacgtggcgt
    40321 taaagcagat tccgtattga tagtaaccat tttttcctgc cagcagagag taagacattg
    40381 tgaaaaaata cgtgaacaat tcacacaggt gtcaggtcgg aaacagcata aatatggatt
    40441 aaattgctgc gacatgccgt tatgtgatgg atattccaat tttaaaatta agttgaatta
    40501 ttgagattat tattaacctc ctaattttaa agcagataaa attcataaag ttcattaatt
    40561 gataattgat atggattatt tcataaccat gatatggatt atgatgatct agagatataa
    40621 aaaaccctgc catgcggcag ggtcataaaa gtaagaggaa tgaattaacg cgccagccaa
    40681 ccgccatcca cagcaatggt ataaccattt acataatctg aagcgctgga ggcaagaaac
    40741 actaccggcc ccatcaggtc actcggcagt ccccaacggc cagctggaat gcgatcgaga
    40801 atttccgcgc tacgttgttc atctgcccgc agttgttggg tattgttggt cgccatgtaa
    40861 cccggagcta tcgcattaac attaatgttg tgtttagccc attcgtttgc catcaatcgc
    40921 gtcacaccca tcacgccgct ttttgatgcg gtataagaag gcacacggat cccgccctgg
    40981 aaggagagca ttgacgcgat attgataatc ttgccgccat tgccttgcgc gataaagtgt
    41041 ttcgccgctg cctgagacat gaagaatacg ctcttgatat tcaggttcat gacatcgtcc
    41101 cagtcttttt cgctgaactc gagagcgtct tcgcggcgaa tcaatccggc gttattcacc
    41161 aggatatcaa tatgaccaaa ttcagctacc gcgcgatcca gcagtccagg aatgccatca
    41221 atctttcgca gatcggcggt caggcttaaa aaacgacgcc ccagcgccgt gacctgctcg
    41281 atggtttcag tcggttcaac gatgttaatg ccaacaatgt cacagcccgc ttgcgccagc
    41341 cccaacgcca tcccctggcc cagcccagta tcacaaccag tgacgaccgc aactttacct
    41401 tggagagaaa atgcacttaa aatcataaca acaccttatt cgtttatgcc cacaactagc
    41461 gcaaatcttt aacgcccaca tggtccatat cattaaagac ctggttttca ccgaccatgc
    41521 cccagataaa ggtataagct ttggttccga cgccggaatg gatcgaccag ctcggggaga
    41581 tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc ttgcggctgc cccatcatgt
    41641 ggaaaacgca ggcgtcatca tccatattga aatagaaata aacttccatc cggcgctcgt
    41701 gggtgtgaca cggcatggtg ttccacaagt tacctggagc cagctccgtc agccccatac
    41761 tcaattgaca ggtttccagt acatccggga caaaatattt gttaatcgtg cgacggttac
    41821 tggtgaggtt atcgcctaac gtgactggag atacttcgtc cggtgtgact tttttggtgg
    41881 gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt cgccggagtg gcggtatcaa
    41941 tactggcaaa gacaacttct tttgcacctt taccaacata cagggcgtcg cggtgaccga
    42001 tttcatagca ttgaccatcg acagtaatcg taccggcacc gccaatattg ataacaccta
    42061 actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt accaacttcc ccgccaacgg
    42121 aaaccgtttt agttatcggc ataatgccgc caacaataat tcggtcaatg tggctgtaaa
    42181 ccatggtgta ctcatcggcg acaaatacct tttcaaccaa aaattcattg cgcagccctt
    42241 gggtatccag cgtttttgcg tgcgcaccgt ggatgctctg tcttacgtcc acatcaacct
    42301 ccgataatca gtgaagcgtg aaaatagaaa acgatagtga aacactgttt cgttttctct
    42361 ataaagggat gatatgccca tgggctaggg ttttcaatta aaaataaaac aatgttttat
    42421 ttttaattta aggattaaaa aaagtgcagt gtcgatcacg aatatgtcat gagaatggaa
    42481 aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc atcattattc aacagattaa
    42541 tgacggcaga tatttatcgg taaggaggaa atgcagagct actcgtcacg ttcaatggtc
    42601 aatgccacgc cctggccccc gccgatacaa agcgttgcca gccctttgcg ggcattacgt
    42661 ttcaccattt catgaaccag agaaaccagg attcggcaac cggaagcgcc tatcgggtga
    42721 ccgagtgcga tcgcgccacc attgacattg acccgacgct catcccactc aagcatcttg
    42781 ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct cgataagatc gacatcagcc
    42841 aactgccagc ctacacgctc caggcaacgg cgggtcgcat acaccggcgc aattcccatc
    42901 aatgccggat ctacaccaac gctggcaaat gcgcggatgc gggccagcac gggtaaattc
    42961 aacgctcgtg ctttggcttc gctcatcatc attaccgcag ctgcgccatc gtttatggat
    43021 gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg aaggatttaa acgggctaaa
    43081 ccttctgcac tggcgtcagt gcgtggctgt tcatcggtat caacaaccaa cgtctgaccg
    43141 ttactttgga tcattaccgg gacgatctca tctttaaatc gtccggcgtc aatcgccgct
    43201 cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca actgacggct gatgccatat
    43261 tcgcgagcca gattttcggc ggtgacacca atatgataat cattgaaggc atcccacaac
    43321 ccatcatgca ccagactgtc aaccaactgg ctattgccaa gctgttcacc ggtgcggcta
    43381 tcagtcagaa catgtggtgc gcggctcatg ttttcctggc cacctgcgat gacaatatca
    43441 gcctcgccac actgtatcgc ctgagtagcc agatgcagtg ctttaagacc ggaaccgcaa
    43501 acgtcattaa tcgtgattgc agaaacgctg ttaggaagac cacctttaat agccgattgc
    43561 cttgccggat tctgccctgc ccctgcagtc aacacctgac caagaattac ttcatccacc
    43621 gcatatgcag gaacgccggt acgttctatt aacgctttca cgaccagact accaagttcc
    43681 acggcggaat gacccgctaa cgcaccacga aagcagccga taggtgtccg taacgccccg
    43741 acaatcacaa cgtctttcat cacaacctcg caccaattaa cagtgcaata gtaaatgatt
    43801 gttaattaca gttatcttaa ttgtttaaaa aaagtgattt ttatcacaaa ggaaatatgc
    43861 ctgagcagta gtcagagtca taactggcac gtaaggtttg caaccactaa cccaccaata
    43921 gatgggtaga tagggcgtta atctcccata cttaacttgg tttatggtaa attgccctcc
    43981 attttgttta atttgtagat gatacgttca gataatgtct aatatttggt ctaaagaaga
    44041 aactctgtga agtttcgcgc tctatggcac agccgttgat gcaggcacgc tattccttcc
    44101 tattcagtta ggttcggcag gggctgtggt cctgtttatt actgctctgg tcgcctggcc
    44161 tttaacatat tggccacata aagccttatg ccagttcatc ctctcatcaa aaacatcagc
    44221 aggtgaaggg ataacgggcg cggtaacaca ctactatggc aagaagattg gtaatctgat
    44281 taccacgctg tacttcatcg ccttttttgt cgtcgtgttg atatatgcag tggcaattac
    44341 caactcactt acggaacagc tggcaaagca tatggttatt gatcttcgca tccgtatgtt
    44401 ggtgagtctg ggtgttgtat taattctgaa tctcattttt ctgatgggac gtcatgccac
    44461 tattcgggta atgggatttt tggtattccc attgattgcc tatttcttat ttctttccat
    44521 ttaccttgtc ggtagttggc aacctgatct attaacaacc caggtagagt tcaatcagaa
    44581 tacccttcac cagatatgga tatcgattcc cgtgatggtt ttcgccttta gccatacgcc
    44641 cattatttct acgtttgcca tagacagacg tgaaaaatat ggcgaacacg ctatggataa
    44701 atgcaaaaaa attatgaaag tcgcttatct catcatctgc ataagtgtac tgttctttgt
    44761 ctttagctgc ctgctttcta ttccatcttc gtatattgaa gcggctaaag aagaaggggt
    44821 caccatttta tcggcgcttt ctatgctgcc gaacgcccca gcatggttgt caatttccgg
    44881 gattattgtc gcagtagttg cgatgtcgaa atcattcctg ggtacgtact ttggcgttat
    44941 tgaaggtgcc acagaggtcg tcaaaacaac actacagcag gttggtgtaa agaaaagtcg
    45001 tgcatttaac cacgcactat caattatgtt ggtatcgctg attaccttca ttgtttgttg
    45061 cattaacccg aacgcgattt cgatgattta cgcgatcagc ggcccgctca ttgccatgat
    45121 acttttcatc atgcctacgc tgtcaacgta tctcatcccg gcgcttaaac cctggcgttc
    45181 catcggaaat ctgattacgc tgatcgtggg tatcctgtgc gtatcggtaa tgttctttag
    45241 ctaatatccg ttgtggatgg ttcaaaattg ctacaatttt gaaccattcc atttttcata
    45301 atattcattt gttattattc cgccctatca cttctctcgt catatcctag aattatcagt
    45361 tagttaaaca cacaaagaga tctgaacatc ttaaataaga ctatttaaga tgcataactt
    45421 agattcgcaa gatatcttct ggtcattcaa aaacaatttg cgatatagat attctcatac
    45481 catgagatag ttatctggac cacgttcgac catgcaagat taaatcagtg aaacataatc
    45541 atatttaata acgagaaatg catttttaaa tatcttaatc agaattatag caaatacaga
    45601 ttaatcgcaa tttttcccag caaaattaca aaatcaaggt gtattgacat catcaatcac
    45661 caatgaaatt atgcaattat atacggatag ggaggttctt aacatggggc tttgtagtcg
    45721 ttataaaagt cttacatgca atagttgctc aatgcattgc caaataatgc cagaagagtc
    45781 accgcgtttg cagtactgcg caaactcgt
//
LOCUS       DAZSNM010000010        45162 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZSNM010000010 DAZSNM010000000
VERSION     DAZSNM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45162
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.010"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(61..1056)
                     /gene="gntR"
                     /locus_tag="ACHW42_000474"
     CDS             complement(61..1056)
                     /gene="gntR"
                     /locus_tag="ACHW42_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR8942436.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(1280..1975)
                     /gene="yhhW"
                     /locus_tag="ACHW42_000475"
     CDS             complement(1280..1975)
                     /gene="yhhW"
                     /locus_tag="ACHW42_000475"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR8942437.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(2098..3135)
                     /locus_tag="ACHW42_000476"
     CDS             complement(2098..3135)
                     /locus_tag="ACHW42_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR8942438.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            3468..3956
                     /gene="yhhY"
                     /locus_tag="ACHW42_000477"
     CDS             3468..3956
                     /gene="yhhY"
                     /locus_tag="ACHW42_000477"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR8942439.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(4015..5757)
                     /gene="ggt"
                     /locus_tag="ACHW42_000478"
     CDS             complement(4015..5757)
                     /gene="ggt"
                     /locus_tag="ACHW42_000478"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR8942440.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            5877..6317
                     /locus_tag="ACHW42_000479"
     CDS             5877..6317
                     /locus_tag="ACHW42_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR8942441.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(6304..7047)
                     /gene="ugpQ"
                     /locus_tag="ACHW42_000480"
     CDS             complement(6304..7047)
                     /gene="ugpQ"
                     /locus_tag="ACHW42_000480"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR8942442.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(7044..7977)
                     /locus_tag="ACHW42_000481"
                     /pseudo
     CDS             complement(7044..7977)
                     /locus_tag="ACHW42_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(8036..8881)
                     /gene="ugpE"
                     /locus_tag="ACHW42_000482"
     CDS             complement(8036..8881)
                     /gene="ugpE"
                     /locus_tag="ACHW42_000482"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR8942443.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(8878..9765)
                     /gene="ugpA"
                     /locus_tag="ACHW42_000483"
     CDS             complement(8878..9765)
                     /gene="ugpA"
                     /locus_tag="ACHW42_000483"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR8942444.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(9863..11179)
                     /gene="ugpB"
                     /locus_tag="ACHW42_000484"
     CDS             complement(9863..11179)
                     /gene="ugpB"
                     /locus_tag="ACHW42_000484"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFR8942445.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(11576..12289)
                     /gene="livF"
                     /locus_tag="ACHW42_000485"
     CDS             complement(11576..12289)
                     /gene="livF"
                     /locus_tag="ACHW42_000485"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR8942446.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(12291..13058)
                     /gene="livG"
                     /locus_tag="ACHW42_000486"
     CDS             complement(12291..13058)
                     /gene="livG"
                     /locus_tag="ACHW42_000486"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR8942447.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(13055..14332)
                     /gene="livM"
                     /locus_tag="ACHW42_000487"
     CDS             complement(13055..14332)
                     /gene="livM"
                     /locus_tag="ACHW42_000487"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR8942448.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(14329..15255)
                     /gene="livH"
                     /locus_tag="ACHW42_000488"
     CDS             complement(14329..15255)
                     /gene="livH"
                     /locus_tag="ACHW42_000488"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR8942449.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(15303..16412)
                     /gene="livK"
                     /locus_tag="ACHW42_000489"
     CDS             complement(15303..16412)
                     /gene="livK"
                     /locus_tag="ACHW42_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR8942450.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            16836..17219
                     /gene="panM"
                     /locus_tag="ACHW42_000490"
     CDS             16836..17219
                     /gene="panM"
                     /locus_tag="ACHW42_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR8942451.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(17418..18521)
                     /gene="livJ"
                     /locus_tag="ACHW42_000491"
     CDS             complement(17418..18521)
                     /gene="livJ"
                     /locus_tag="ACHW42_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR8942452.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(18792..19646)
                     /gene="rpoH"
                     /locus_tag="ACHW42_000492"
     CDS             complement(18792..19646)
                     /gene="rpoH"
                     /locus_tag="ACHW42_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR8942453.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(19891..20949)
                     /gene="ftsX"
                     /locus_tag="ACHW42_000493"
     CDS             complement(19891..20949)
                     /gene="ftsX"
                     /locus_tag="ACHW42_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR8942454.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(20942..21610)
                     /gene="ftsE"
                     /locus_tag="ACHW42_000494"
     CDS             complement(20942..21610)
                     /gene="ftsE"
                     /locus_tag="ACHW42_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR8942455.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(21613..23112)
                     /gene="ftsY"
                     /locus_tag="ACHW42_000495"
     CDS             complement(21613..23112)
                     /gene="ftsY"
                     /locus_tag="ACHW42_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR8942456.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEEAAKEEITDEELEAQA
                     LAAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFIS
                     LFRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEE
                     MGEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTF
                     RAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNK
                     SHLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKL
                     DGTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23262..23858
                     /gene="rsmD"
                     /locus_tag="ACHW42_000496"
     CDS             23262..23858
                     /gene="rsmD"
                     /locus_tag="ACHW42_000496"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR8942457.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            23848..24117
                     /locus_tag="ACHW42_000497"
     CDS             23848..24117
                     /locus_tag="ACHW42_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR8942458.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(24120..24479)
                     /locus_tag="ACHW42_000498"
     CDS             complement(24120..24479)
                     /locus_tag="ACHW42_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR8942459.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            24620..25246
                     /locus_tag="ACHW42_000499"
     CDS             24620..25246
                     /locus_tag="ACHW42_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR8942460.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            25320..27518
                     /gene="zntA"
                     /locus_tag="ACHW42_000500"
     CDS             25320..27518
                     /gene="zntA"
                     /locus_tag="ACHW42_000500"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR8942461.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(27620..27865)
                     /gene="tusA"
                     /locus_tag="ACHW42_000501"
     CDS             complement(27620..27865)
                     /gene="tusA"
                     /locus_tag="ACHW42_000501"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR8942462.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            28086..28751
                     /locus_tag="ACHW42_000502"
     CDS             28086..28751
                     /locus_tag="ACHW42_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR8942463.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            28824..29381
                     /gene="dcrB"
                     /locus_tag="ACHW42_000503"
     CDS             28824..29381
                     /gene="dcrB"
                     /locus_tag="ACHW42_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR8942464.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(29385..30602)
                     /locus_tag="ACHW42_000504"
     CDS             complement(29385..30602)
                     /locus_tag="ACHW42_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8942465.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            30736..31785
                     /locus_tag="ACHW42_000505"
     CDS             30736..31785
                     /locus_tag="ACHW42_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR8942466.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            31840..32427
                     /gene="acpT"
                     /locus_tag="ACHW42_000506"
     CDS             31840..32427
                     /gene="acpT"
                     /locus_tag="ACHW42_000506"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR8942467.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            32538..34112
                     /gene="nikA"
                     /locus_tag="ACHW42_000507"
     CDS             32538..34112
                     /gene="nikA"
                     /locus_tag="ACHW42_000507"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8942468.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            34112..35056
                     /gene="nikB"
                     /locus_tag="ACHW42_000508"
     CDS             34112..35056
                     /gene="nikB"
                     /locus_tag="ACHW42_000508"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR8942469.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            35053..35886
                     /gene="nikC"
                     /locus_tag="ACHW42_000509"
     CDS             35053..35886
                     /gene="nikC"
                     /locus_tag="ACHW42_000509"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR8942470.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            35886..36650
                     /gene="nikD"
                     /locus_tag="ACHW42_000510"
     CDS             35886..36650
                     /gene="nikD"
                     /locus_tag="ACHW42_000510"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR8942471.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            36647..37453
                     /gene="nikE"
                     /locus_tag="ACHW42_000511"
     CDS             36647..37453
                     /gene="nikE"
                     /locus_tag="ACHW42_000511"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR8942472.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            37459..37860
                     /gene="nikR"
                     /locus_tag="ACHW42_000512"
     CDS             37459..37860
                     /gene="nikR"
                     /locus_tag="ACHW42_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR8942473.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(37980..38339)
                     /locus_tag="ACHW42_000513"
     CDS             complement(37980..38339)
                     /locus_tag="ACHW42_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR8942474.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(38336..38611)
                     /locus_tag="ACHW42_000514"
     CDS             complement(38336..38611)
                     /locus_tag="ACHW42_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR8942475.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(38670..39794)
                     /locus_tag="ACHW42_000515"
     CDS             complement(38670..39794)
                     /locus_tag="ACHW42_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8942476.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(39794..42529)
                     /gene="rbbA"
                     /locus_tag="ACHW42_000516"
     CDS             complement(39794..42529)
                     /gene="rbbA"
                     /locus_tag="ACHW42_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR8942477.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(42526..43593)
                     /locus_tag="ACHW42_000517"
     CDS             complement(42526..43593)
                     /locus_tag="ACHW42_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR8942478.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(43656..43769)
                     /gene="yhiY"
                     /locus_tag="ACHW42_000518"
     CDS             complement(43656..43769)
                     /gene="yhiY"
                     /locus_tag="ACHW42_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR8942479.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            complement(43959..>45162)
                     /locus_tag="ACHW42_000519"
     CDS             complement(43959..>45162)
                     /locus_tag="ACHW42_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR8942480.1"
                     /translation="NDNANDVAVTESYKPIWNWEISQYNVFDIKFETIIKPQFADRTY
                     FSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFATGHLGIMAEGENFLSQLLNIEDEVL
                     NVLLRENFNEQLDTNVNTIISILNRRDNVLESLQPYLVINKDAVTPCTFLGDQTGDRF
                     SNICGDQFIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYDGIKD
                     YPVCFYDPKYKIMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAEIINKKHH
                     AIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRE
                     RIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLI
                     RRRTMLQL"
BASE COUNT        10481 a        12521 c        11585 g        10575 t
ORIGIN      
        1 agaagcaata tccatccgtg ccttaagtgt ataagtgtga gctacttcaa atttgtgggc
       61 ttaaatagat ccgcccggtg acaaggtgaa acctaaatct aacattttcg gtgtcacaga
      121 ttcgccacga atacgcgcca gcaggcgttc agcgccaata ctgcccatcc gctcacgcgg
      181 cgtcagcacg ctcgcaagtc gtggctccat cacctgacca atgtcatgac cgtggaaacc
      241 ggcaatcgcc atatcgtcag gaacttttaa ccccagacgc tgacattcaa acgccgcgcc
      301 gaccgccaga tcatcattag tacagaacac gccatccagc tgcggatatt cccgccgcgc
      361 ctggcgaatc agttcaatac cggaagagta agaagaagat tgctcaacca tcacgctata
      421 tggcaccagg cctgcatcca gcatcgcctg ttcgtatccc ttctgtttga tgatagtacg
      481 ttcgtcgaga cgtgcgccga gataggcaat gtggcgatgc ccacgagcaa taatggcggt
      541 agtcatctgg cgtgccgctt caaagttatc aaaaccgacg gcgatatcga ggcacggcga
      601 ctggctgtcc atcaactcca ccaccgggat ccccgccact tcaatcatct ttaaggtgcg
      661 cggcgtgtgg gtacgttcgg tgaggatcag gccgtcgata ttccaggaga gcatggattc
      721 gagacgttct tgctccattt ccggtttata accgtagtgc gccagcatgg tctgataacc
      781 gtgcgcgtcg gtgacgcttt cgattccgcg taatacttcc gcgaaaacct ggttggtgag
      841 agaaggtaac aggacgccaa tcgcccggct ggtagcgtta gagaggatat cgggcgcacg
      901 attgggaata tagcccagtt catcaagagc ggcggcaatc ttgccgcgta gagcgacgga
      961 aacctgctcc gggttgcgta aaaaacggct gaccgtcatt ttggtcacgc ctacacggtc
     1021 agccacatcc tgaagtacgg gtcttttctt tttcatcgtc ctgaaggtac aaaagagata
     1081 gattctctta gtttaacacg gacgcatcag aaccttcccg gggaaaacag tgcttgttta
     1141 caattattag atatacacta aatcaattgc atcgcattgt gctaacgacg cggcaaaacc
     1201 ccggaagcgt gcatcagcac gcgaccggag cgagcgagga agcctgcaaa aaggcaggct
     1261 tcaaagatgc cgttgagttt taaaccggcg gcagatcgaa cagtaacact tcgctgtcgc
     1321 tatccgcatg gatggagatt gcctgctcat cccagattgc cagaccatcg ctggtcgagg
     1381 ctttcacgcc gttaatggta acattgcctt ttaccacctg aatccagacg cggcgttcag
     1441 cggcaatctg atgcaccgac tgctcatctt tcagcaacgc ccaacgatac agttccatat
     1501 cctgatgcac tttcagcgaa ccatctcgcg catccggcga gagcaccagc tgtttgccct
     1561 gcacggcatc gaagcgacgc tgttcataac gcggcgtaat gccattttct tcaggcatga
     1621 tccagatctg atacagatgc agacgctcgg tgctgcttgg gttgtactct gagtgacgaa
     1681 tacccgtacc cgcactcata atctggaact cacccgccgg aacctgctct ttattgccca
     1741 tgctgtcctg atgctcaaca gtaccttcca gcacgtaggt caaaatttcc atatctttat
     1801 gcgggtgagt gccgaagccc tgccctactt caatcacgtc gtcgttaatc acgcgcagcg
     1861 cggagaagcc cataaagttc ggatcgtaat agttggcaca agagaaagta tgccaggagt
     1921 ccagccagcc atgatttgca tgaccacgtt catttgcttt gcgtaagtag atcatttgta
     1981 ttcgccccct gaatgatttc gatgggctta gtgtggaccc gatctgcctg ggatgataga
     2041 gggtgaaaat tgccccctct gttcaaaaaa attgaactat tcgaggggcc atcaaactta
     2101 attcgcgaga gtcactgtgg agggagaggc ttgctcaaat ccgcgttcaa ggatttccag
     2161 attggtaaga acttcagatt ccttgacgta atttggcgca ccgtgggtga tggtttgata
     2221 caacgcatca taaacacgcc cgtaatcgcc catctccggc ttcatctctt ctctgaccgt
     2281 cacaccctcg tcattgacat actccagcac accgaccgaa tcatccgctg cgaatcccgg
     2341 ttcgcccggc ataatattag ccttcaggct ggtttcctgc tggtcgatac cgtatttaat
     2401 aaacgaacct ttcttaccgt gaacgataaa tttcggataa tcgattttca ccagatggct
     2461 ggttttgacg atggctttca ggtcgccata aaacagttgc gcttcaaagg tgtcgtcagg
     2521 attggcttta ttacgcaggc tgcggatgtc ataagcgacg tgatccgggc gaccgaacag
     2581 agaaataatc tggtccatcg tatgcacacc aaggccatag aacgcgccat cctgcggcag
     2641 tccaggtttg gtttctgcca ccgggcggta atagtcaaaa tggctttcca cttcgacaat
     2701 ctctcccagc ttgccgcttt caatcgcttt tttcgccgtc aggaagcagg aatcaaagcg
     2761 acgattctga tatggcgtga cggtcagccc tttgcttttc gccagtgcaa acagctcttt
     2821 cgcctgcgca agtgtcggag tgaacgtttt ttcgaccagc acatttttcc cggcttccag
     2881 cgcgcgcttc gcgtactcga agtggctgtc cgcatgggtg cagacaacaa ccaacttaac
     2941 atcgggatcg tttagcactt cgtcgagatc gctggtgaaa tggatatgag agtaaatggg
     3001 agcctgttct tccggcttcg catggcgacg aaaaatatgc gcgacatgcc agctatcctt
     3061 gcggttaagt acatacggca gatggtaacg ggtggtgctt ttgccgaagc caataaaggc
     3121 gcagttgatg accatgattt cgtccttttt aaggtggtta tttacacctt agcgcaaagc
     3181 ggacgtggtt cctactggag tgcgcataac gaacacaagc actcccgtgg ataaattgag
     3241 aacgaaagat caaaaaaaaa gccagcaccc ggctggctaa gtaatactgg aagcaatgtg
     3301 agcaatgtcg tgctttcagg ttctccgcga gggtcttcct gatcgcgata caataataat
     3361 cattctcatt cgcacttgtc caacactttt tgcaaaaaaa tgcatttgac tcgcatttga
     3421 aagtcaatga tgttgaaagg gacatttacc ccaaagagga caaaggaatg agtgagatag
     3481 taatacgcca cgcagaaaca cgggattacg aggccatcag gcagattcac gcccagccgg
     3541 aggtgtattg caacacacta caggtgcctc atccttccga tcatatgtgg caggagcgac
     3601 tcgccgatcg tcccggcatc aagcaactcg tcgcctgtat tgatggagac gtcgtgggcc
     3661 atctcaccat tgacgtgcaa cagcgcccac gccgcagtca tgttgccgat tttggtatct
     3721 gtgtcgactc tcgttggaag aaccgcggcg tcgccagcgc cctgatgcga gagatgattg
     3781 aaatgtgcga caactggtta cgggtagatc gcattgaact aaccgtgttt gtcgataacg
     3841 cgccagcaat taaggtctat aaaaaattcg gctttgagat tgaagggacc ggtaagaagt
     3901 acgcattgcg taatggtgaa tatgtcgatg cgtattatat ggcgcgggtg aaatagagaa
     3961 tttgcaatct aagtttgtca tttaagccct cacctaccgg gagggcttaa gatattaata
     4021 ccccgccgtt aaatcatcca ccgagcgcgg gtcggatgcg ccgtacaact caccgtccgg
     4081 cccaaccata atgctttgtg tgctgcccat cgcctctttc agcgccactt tctgaccttt
     4141 tgcttccagc agcttgagcg tatccgggct aaaccctttt tcgacacgca gctcgtccgg
     4201 caaccactga tggtggaaac gcggcgcatt ggtcgcttcg gcgacgttca tgccataatc
     4261 gatgctattc accaccattt gcagcaccgt ggtgataatc cggctaccgc ctgggctacc
     4321 ggtaaccagc caggttttac cgtctttcac cacaatggtc ggcgacatcg acgacagcgg
     4381 gcgtttgttc ggcccgacgg cgttggcatc accgcccacc agcccgtaaa cgttcggtac
     4441 gcccggtttg gcggagaaat catccatctg gttattaagc agaataccgc tctcacccgc
     4501 gacaataccc gtaccgaagg tggtgttcag cgtataggtc accgccaccg cgttaccgtc
     4561 tttatccact acagagtaat gggtagtttg attactctca taaggcgcaa gcttgccagg
     4621 gcgaatctca ctggacggct tcgctttttt gatatcaatt tgatcggcaa tggatttggc
     4681 ataggcttta ttggtcagtg cctgccacgg gactttgaca aaatccgggt cgccaagata
     4741 ttccgagcgg tcggcgtagg cgtatttctc cgcttctgcc atgatttgca tcgcatcggc
     4801 gctgccaaag ccgtatttct tcatatcgaa gttttccaga atattgagga tttgtacgat
     4861 atggatcccg ccggaggatg gcggtggcat ggagtaaacc tgatacccgc gataatcgcc
     4921 gcttatcgga gtgcgttcga ccgctttata ggccgctaaa tcttctttag tgatcaagcc
     4981 accgtttttc tgcatctcct gggcgatctg ttccgcaatc gtgcctttat agaattcgtc
     5041 cgggccgttt tcagcaatca tctccaggct ctttgccagg ttcgcctgca ccagcgtgtc
     5101 gccctttttc agcggctcgc cctctttcca gaagatagct ttactgtttt cgtgattcgg
     5161 caacacttcg ctaccgtagg ttttgagatc gtcagccagc gcgtcgttaa cgataaaacc
     5221 atcgcgtgcc agtttaaacg cgggctgcac gactttgttc agcggcatgg tgccgtattt
     5281 atccagcgcc agcgagaaac ctgctaccgt acccggtgtg ccggaagcca gatgcgaagt
     5341 gagtgatttt ttgctgtccg ggttgccctg atcatcgagg aacatatcgc gggtcgcttt
     5401 ggcgggtgcc atttcgcgga aatcgatagc cgtggtattg ccatttttcg agcggattaa
     5461 cataaaacca ccaccgccca gattccctgc ctgcggatgc gttaccgcca gcgcgtagcc
     5521 caccgccacg gcggcatcaa cggcattccc gccctccttg aggatatcca cccccacctg
     5581 agtggcagtg gcgtccacag acgctaccat tccctgtttc gcgcgtaccg gatggaagac
     5641 atcttcctcc acaccatacg agacgggcgg cgcaggaggc gcggcggcgg cgctaaaaca
     5701 acttcctgag agcagagcag caatggccac ccggcgtaaa aacgtcggtt ttatcatcgt
     5761 tattctccag agattaaggg gcaaccccag ctaagcctgg tatataactc tgaattaatc
     5821 atcgttttgt cgggaagcga gtaaacttaa aggatatctt cagaggagga tacgcgatga
     5881 aacgacttct gattcttacg gcactcctgc cgtttgtcgg ctttgcacag cccattaata
     5941 ctctgaacaa ccctaaccag ccggggtatc agatacccag ccagcagcgg atgcaaaccc
     6001 agatgcagac tcagcaaatc cagcaaaaag ggatgctgaa tcagcaactg aaaacgcaaa
     6061 ctcagttgca acagcagcat ttagaaaacc agataaacaa taattctcag cgggtgttgc
     6121 agtcacagcc gggggagcga aatcccgccc ggcagcaaat gctgcccaac accaacggcg
     6181 ggatgttaaa cagcaaccgt aatccggata gttcgttgaa tcagcagcat atgctgccgg
     6241 agaggagaaa cagcgacatg ctgaatcagc ccagcacgcc gcagcctgac attccgttga
     6301 aaactattgg gccgtaaagt tcggaccaat cacgtcaatc gcatcggtac agatgcaatc
     6361 cacgccccag cgcagcaact ctgccgcgtg ctggggttta ttgacggtat aaaccagaat
     6421 ccgtaatccg gcgtctttca actgcatcac tcgcgcttta tcgagtaact tatgattgag
     6481 atgaatagag acgcagccca gccgcgcggt cagttcgcgc cagtcgtcgc gccactcatc
     6541 aagcaataaa ccacgcggca gttccggtgc cgcctgttgt gcagcttcta aagcatcaat
     6601 ctcaaacgat gacagcagcg gcggcgtcat accggcccac agttggcgtg ccgccagcgc
     6661 caccattttg cccgttaatg gtccagtgcc agtggtgggt ttgatttcga tattcgccat
     6721 catcccgtgt tcgcgacagc gttccgccac ctgcgaaagc aacggcagcg gctcaccttt
     6781 aaacgctttg ctgtaccaac tgcccgcatc cacgcgcagt aaatcctgcc agttcagttc
     6841 acccgcgacg ccccagccgt tgctggtgcg ttcgagattg tcgtcatgga gcaggaagat
     6901 ctcgccatct ttcgataact tcgcgtcaaa ttcgatcatc ttatgaccgt attttgcccc
     6961 gacgtcgatt gccgccaggg tgttttccgg ggccagctta ccgccgccac gatgagcgac
     7021 gatgcgggga taaggccagt tactcatact cgttgtcctg tttcaccatc aaaaagatgc
     7081 agctgatttt ccgccagatg cagccacagc gtgctgcctg ccgtcgggcg ctcctgatgc
     7141 gccagtcgca ccaccagctt ctgcttgcga gcttagcgca atatgttccg ggcgaatgcc
     7201 gagggtcatt ttacgcccgg catactgacg gtagccaccg tttagcggta gcacgatacc
     7261 gccgtccagt tcgaaatgcg tgccttcatt attcatgcgg cctgtcagca ggttcatcgc
     7321 cgggctgccg ataaaactcg ccacaaacag gctggcgggc ttttcgtaga cttccaccgg
     7381 cgtgccaatc tgttcggcaa cgccgccgtt catcaccatt actcgctggg cgagcgtcat
     7441 cgcctcaacc tgatcgtgag taacgtagag tgaagtcgtt ttcaggcgac ggtgcagctg
     7501 ttgcagttca agacgcatct gtacgcgcag cttggcatcg aggttagaga gtggctcatc
     7561 aaacaagaac accgccggat cgcgcacaat cgcgcgtccc atcgccacac gctggcgctg
     7621 accgccggaa agctcgcgcg ggcgacgttt gagcagaccg tccagctcca gaatgcgcgc
     7681 cgcttcttta acgcgctcgg ctatttgctg cttgcccatg ccgcgaattt tcagccccca
     7741 cgccatgttt tcttcgacgc tcatatgcgg ataaagcgcg taattctgga acaccatcgc
     7801 aatcccgcga tctttcggct ccatttcggt cacgcgctgg tcgttgatcc agatatcgcc
     7861 ttcggtcacc ctctccagcc cggcaaccat acgcagcagc gtcgatttcc cgcagccaga
     7921 cggcccgacc atcacgataa attcgccatc cgccacatca agggtcagcg gtttaatgac
     7981 ctaggttttg ccatcccagc ttttggttac tgcctgtaat ttcagtcctg ccatcttatt
     8041 tctcactatc gaccaggccg cgcacgaagg cacgctgcat cactaaaaca atcactactg
     8101 gtgggatcag ggttaacaac atcgccgcca tcactgagtt ccattccgtg gtgccttcgc
     8161 cagtggcgat catccctttg atccctgcca cggtggtgcc gagatccacg tcggtaataa
     8221 tcaacaacgg ccacaaatac tggttccagc cgtaaataaa ggtgatcaca aacagcgccg
     8281 ccagattagt tttggagagc ggaaaaacga tgtcgcaaaa gaagcgcatt ggtgacgcac
     8341 cgtcgatccg cgccgcttcc accagttcat ccggcagcgt cataaagaac tggcgaaaca
     8401 gaaacgtggc ggtcgccgag gccatcagcg gcagcgttaa accggcgtag ctgtcgagca
     8461 tcttcaggtt ggcgatgact tccaccgtcg ggaagatacg tacttcaacc ggcagcatca
     8521 gggtgataaa aatcatccag aagaagaggt tacgtagcgg aaaacgaaac cagacaatgg
     8581 caaatgccga gagcatcgag acagtaattt tgccgagcgt aatgctgaac gccatcacaa
     8641 agctgttaag caacatccgc cagaacggcg cgctatttgt gcctaccccg ttcacccaga
     8701 tgttgtggat gttttccagc agatgcgtgc cggggatgag cgtcatcggc gcggcgtaga
     8761 cggcctgttt atccagcgtc gccgcgacaa acgccacgta cagcgggaag aggatcaccg
     8821 cgatcccgag gatcagcatg gtatggctga atatcgtcag ccacggacgg ttctcaatca
     8881 ttggtaacgc accttgcttt caacatagcg gaactgcacc accgtcagca cgatgacgag
     8941 gaacatcagc accatcgact gtgcggcaga cgaagccaga tccagtccgg taaaaccttc
     9001 gcggtagatc ttataaatca gcgtcgtggt ggcctgaacc ggcccgccgg acgtggcggc
     9061 gtcgatcacc gggaaggtgt cgaagaaggc atacaccagg ttcactacca gcaggaagaa
     9121 actcaccggg gcgataagcg gcagcgcaat cttaaagaag cggcgaatcg gccctgcacc
     9181 gtcgatggct gcggcttcga tcaacgaacg gggaatggat tgcagcgcgg catagaagaa
     9241 cagaaagttg tagctgattt gcttccacac cgaggcaaac accaccagaa acatcgcctg
     9301 accgctattt tgcgcgtggt tccagtcgta gccgaactca gcgagaaaat gggtgatcag
     9361 cccgcgaccg gggttaaaca ggaagatcca caatacggcg gcaacggcgg gagccacggc
     9421 atacggcagc aacattaagg tttggtagaa acgactgccg cgcacgatgt actccaccag
     9481 cgccgcaaag aacagcgata ccagcaaacc gctgacggtg acaaaggtgc tgaattttat
     9541 cgtcgtccag aaggagtcga gataatagct gtcatgaaac agcgtgacga agttatccag
     9601 accgacaaac tggctggaga aaccaaacgg atcgacgctt tgtagcgagt accacaacgc
     9661 ttcgcccgca ggccagataa aaaagataac ggtgatgatg agctgcggcg cgaccagcag
     9721 ataaggcagc cagcgcgagc ggaacaccgg acgggatgat gacattacgg ttaattcctg
     9781 aactgtgccg gatgcgcttc gcttatccgg cctacacgcg atgccaggtc agataagcga
     9841 aacgcatccg gcattacact gattaagact tcgtcgattt ctcaaagcgg cgcagtaact
     9901 ggttcccacg ctcaactgcg gtatccagcg cctgctgtgg cgtcttctta ccggtccaca
     9961 cgctctcaag ctcttcatcc acaatcacgc ggatctgcgg catgttgccc agacgaagcc
    10021 ctttggtgaa cggcaacggc ggcttgttca gcatctgacg cgtcgcggta tccgcacctg
    10081 ggtttttctc gtaaaagccc tgctcacggg tcaggtcata cgccgctttg gtaattggca
    10141 gataaccggt tttctgatgc cactcagcag cgttttctgg cttcgcgagg aagtcgagaa
    10201 acttcgccac gccggtatac gtttctttat ctttgccctg catcacccac aggctggctc
    10261 cgccgataat ggcgttttgt ggcgcatctt tcgcatcggc gtcgtaaggc atcataccta
    10321 cgccgtagtt aaatttggcg tactcgcgaa tgttggcaag agaaccggaa gaggcggtgg
    10381 tcatcgcgca atcaccgtta tagaacttct cggtggattc atccttacga ccgacgtagc
    10441 tgaagtcgcc cttcttgttc atctcctcga gcatggcgat gtgtttcacc tgctccggct
    10501 tgttgaactc cagcaccgcg tccgtgccgt caaagccgtt gtttttgctg gcaaacggca
    10561 gaccgttcca ggcgctaaag ttttccagtt ggatccagcc ctgccagccg ctggcgtagc
    10621 cgcacttaat accggaggct ttcagttttg cggaatagtc cgccagatcc tgccaggttt
    10681 tcggcggctg ttccgggtct aaccccgctt tcttgaaggc gtctttgttg taatagagaa
    10741 cggaagtcga gctgttgaac ggctgggaga gtaagtggcc ggttttgctg tcggagtagt
    10801 aaccggaaac cgtcggcaca aactgcgact catcgaactg aatccctgcc tctttaaaca
    10861 cgtcatacac cggtttaatg gctttcgacg ccatcatggt ggcggtgcca acttcataaa
    10921 cctgcaaaat agccggagcg ttgccggtac gaaatacggc aatccccgcg cttaaattct
    10981 gttcgtagtt gcctttatag gtcggtacaa ttttgtaatc cggattttcg gcgttaaaac
    11041 gttgggccag agaatccacc tctttaccca gttccccttc catagaatgc cagaacggaa
    11101 tggtcgtcac tgcctgtgca ttccccatta acgccagtcc gagcgccagt gctgaagctg
    11161 tataatgtaa cggtttcatc gtttatctct cttgttgtac cgaatgcgcg aattcacgcg
    11221 ttttatgctc gcggggtaac atgacatgct cgaattacag aaaaataact tttttgttac
    11281 atttgtaaga tagtaaggtg tcagaaagat gacaaggcgg tgacggcgtg ggtgagggaa
    11341 aatgggagat ggggcacgga taagcggaaa aatagaaggt ctgaatcaaa ctctacagat
    11401 tactcatcgt ttcatgccgg atgcggcgta aacgccttat ccggcctaca agatcgtgca
    11461 aattcaatat attgcaactc acccagtagg cctgataagc gcagcgcatc aggcaatttt
    11521 gcatttgtca cctgtctcaa aggagtcttt tgactcctta tcaatcaacg tgttattacc
    11581 cgcctaaata cgcacttctc actgcttcgt tcgccagcag cgcgtcgccg gtatcggaga
    11641 gcaccacatg gccgttttcc agcacgtagc cgcgatccgc cagctttagc gcctggttgg
    11701 cgttctgctc gacgaggaag atagtcatcc cctgctcgcg cagctgctcg atggtgtcga
    11761 aaatttgctg gatgataatc ggcgcaagac cgagcgatgg ctcatcaagt aacaacaaac
    11821 gcggattgct catcagcgcg cgaccaatcg ccagcatctg ctgttcaccg ccggacatgg
    11881 tgcccgcccg ctgaacacgg cgctcatgca gacgtggaaa cagttcatac acccacttta
    11941 tgcgctcctg aaactggtca cgttcggcaa agaagccgcc catcgccagg ttctcttcca
    12001 ccgtcatccg cgagaagacg cgacgccctt ccgggacaat cgctaccgct tcgcgcatga
    12061 ttttcgccgt ctgccagtcg gtaatgtctt tatcatcaaa cacaattcgc ccgctggtgg
    12121 cgcgcggatc gccgcataac gtgccgagca aggtggtttt ccccgccccg ttcgcgccaa
    12181 tgagagtgac aatctcgccc tgattgatat gcaggctcac ctcatgcagc gcctggattt
    12241 tgccgtagtg ggcgctgact ttgtcaaagg acaacatgac tttttccatc ttatgcctca
    12301 cctaaatagg cacggatcac gtccgggtta ttacggattt gctccggcgt accgtttgcc
    12361 agtggcgtcc cctgattgac cacataaatt cggtctgaaa tccccatcac cagcttcata
    12421 tcgtgttcaa tcaacaaaat agtggtgttg tgatgattgc gcagttcggc aatcagctca
    12481 tccagctctt tggtttcttt tgggttaaga cctgccgccg gttcgtcgag cattaaaatc
    12541 tccggctgcg tcaccatgca gcgggcaatc tccagacggc gttggtcacc ataggccagg
    12601 ttactcgcct gacggttggc gtgttccagc aaaccaatgc gctcaagcca ggtggcggcg
    12661 cggtcgagcg cttcgctctg ggcgcgacgg aaagaaggcg ttttcaacag gccagagaac
    12721 agcccggttt tcagttgctg atgctgcgcc accagcaggt tttcaattac cgtcatttca
    12781 cggaacagac gcacatgctg gaaggtgcgc accacgccca tgcgagcaat ttgctgcccc
    12841 ggtaaacctt caagatgctg atcgcgcagt aaaatggtgc cgccggtggg tttgtagaaa
    12901 ccggtcaggc agttaaaaac cgtggttttt ccggcaccgt tagggccgat taacgagacg
    12961 atttcctgcg ggtacagttc aagattgacg ttgttcaccg ccagcaggcc gccgaagcgc
    13021 atcatcagac cgttaacaga taataatggc tgactcatgc ctgctctcct ttcgctgcgc
    13081 cgtttttcag cttcagttgc gggcgcgtca tgggcagcaa accctgcgga cgccagatca
    13141 tcatcagcac catcaaacca ccgagcatta acatgctgta ttcgttgaaa tcacgcatca
    13201 attcgcgcga caccaccagc agaacagccg ccagaatcac tgcaaattgc gagcccatac
    13261 cgccgagcac cactatcgcc agcacaaagg ccgattcagc aaaggtgaag gattccgggc
    13321 tgacaaagcc ctgacgcgcc gcaaacagcg ttccggcaaa accggcaaat gcggcgctga
    13381 tggtaaaagc agtcagcttg atacggcgcg ggcttaagcc cagcgaacga caggcgatct
    13441 cgtcttcacg caacgcttcc cacgcgcggc ccagcggcat ccgcagcagg cggttaatga
    13501 caaacaggct taacaccacc agcagcaacg ccaccaggta gaggaagata acgcgatcgg
    13561 acggatcgta tttcaggcca aagaaattac tgaacgtgtc ccagccgcct tcacgagcgg
    13621 tacggctgaa ctcgagtccg aagagtgtcg gtttcgggat ctgactgatt ccgttcgggc
    13681 cgccggtgat ttcagtgtta ttgagcagca atatgcgcac aatttcgccg aaaccaaggg
    13741 taacgatcgc cagatagtca ccgcgcagac gcagcaccgg aaaaccgagc aggaaacccg
    13801 ccgccgctgc catcagtccg gcaatcggca aacaggtcca gaaccccaga ccgtaatagt
    13861 gattgagcag cgcaaaagtg taagcgccga tggcgtaaaa accgccgtac cccagcacca
    13921 gcagaccaga aagaccaaca accacattca gcccgagacc gaggataatg tagatcatgg
    13981 tcagagtggc gatatccacc gtcccacgtg acaccataaa cggccacgcc accacaagca
    14041 ccagcagcgc cacgaggaac agcttttgct tcaccgtgga cccatcaatc gcgggcagaa
    14101 taaacttcgg cccggaaacg cttttcaacc ctttctggaa agccggtcgc aaaagctgga
    14161 agaaaaagac caccgccgtg ccgataaaca tccactgcca acggatgtct gaagccgtgt
    14221 cgaccaccag tttggtgcca tccagctcca gttgtacgcc cataaagacg cccgccagga
    14281 caaagaacat cgcggcagag agcagtgcca ttgcaatatg catcggtttc atactttctc
    14341 tacctccggg cgacccagaa taccggtcgg catcaccagc agcaccagaa tcagcagggc
    14401 gaatgagacc acatctttat attccgtact cagataggca gaagagagcg cctccgcaat
    14461 ccccaggatc agcccgccaa tcatcgcccc tggaatgctg ccaatcccac cgagcaccgc
    14521 agcggtaaag gctttcatcc cggccataaa gccgatatag gggttaatga cgcagtagaa
    14581 ctgaccgagc agtacgccag ccactgccgc catcgccgcg ccaataacaa aggtcaaagc
    14641 aatcacccgg tcggtgttga tgccaagcag gctcgccatt ttcagatctt ccgcgcaggc
    14701 acgacaggcg cgccccatac gggaatagcg aatgaaaatc gtcagcgcca gcatggcgag
    14761 gaaggtgaca atccagatca ccgcctgcat ggtggtaata gaggcagaga agttttcgct
    14821 atggcccacc acccactgac cgttaaacag actcggcagc gccacgtcgc gcgaaccttc
    14881 ggtcaggctg acgtagtttt gcaggaagat ggacataccg attgcggaaa taagcgcaat
    14941 caggcgctta gagttacgca ccgggcggta agctacccgt tcgatactcc agccgtaggc
    15001 gctggcgatg acgattgcgc cgacgaatcc cgccgccacc agcagccagc cggtatcaat
    15061 gcccatcatc atcagcgcgg cgatgatcat aaatgagacg tagctgccaa tcatataaac
    15121 ctcgccgtgg gcgaagttga tcatgccgat aatgccgtaa accatggtgt agccgatggc
    15181 tatcagcgcg taggtactgc ccagcgtgac gccgttaaac atctgctgca agaaatacaa
    15241 aaactgctca gacataaggt aacctttcta aacccgcccg cattttacgg gcggtgggat
    15301 gatcacttgg ctgccgtgga tgaaccgtcg gcgtgccact ggaagacacc aaaatcaaat
    15361 cccttaagat cgcctttttc atcccagttc agcggcccaa tcacggtgtt tgcaccgtta
    15421 gcttttaaat ctttcaccag cgccagcggc tcatcgctgc cggtacgctc aagggcagtt
    15481 gccagagatt gcactgccgc gtaagtgatc cagacataag gcccggacgg atctttcttg
    15541 tctgctttca gcgcatcaac gatgccctgg tttgccggat cctggtcata gcgttttggc
    15601 atagtgacca acatgccttc ggcggcatca ccggcaatgt tcgacagcga tgcgttaccg
    15661 acaccttccg gccccataaa ctgggttttc aggccaacgg aacgggcttg gcgcagcatc
    15721 tgccccattt ccgggtagta accgccgtag taaacgaagt cgatattttc ttttttcagg
    15781 cgggcgatca gcgcggagaa atctttctcc ccggcggtaa taccgtcgaa gaagacgacg
    15841 ttggcgttag ccgctttcag cccgtcctgc accgaacgcg ccagcccttc gccatactgt
    15901 tgtttgtcgt gaatgatggc gatgcgctgg ggcttcaccg tctcaagaat gtattttgcc
    15961 gccgtaggcc cctgggaaga gtccagcccg gcagtacgca taatgtgttg ataaccgcgt
    16021 tgggtcagct ccgggttggt cgctcccggc gagatcatca gaataccttc gtcttcgtag
    16081 atatccgacg ctggctgggt agaggaagaa cacagatgac caataacgta tttaatgccg
    16141 tcgttaacga ttttgttggc gaccgcaacg gcttgtttcg ggtcgcaggc gtcgtcatat
    16201 tccacgccaa ccagcttgtc gcccttaatt ccccctttga cattaatgtc tttaatcgcc
    16261 tgacgcgcgc cgttaaattc catatcgccc cactgggcaa tcgggccgga catcgcgccg
    16321 acaacggcga ctttaatatc gtcagccata gcggtgtgtg aaattgccag tgcaatcatc
    16381 cctgcgatga tagttttcgc attccgtttc atagtcaaaa atccccattc gtgatgttgt
    16441 gttgctttgt ttttatgtgt taacaaatca gactgttctt tttttatact gcactgtttt
    16501 tgcctgtctg attaaggggt tagcgcagta ttttgtgatg atagcgatta aaatccctat
    16561 ttttcagtcg attaagaaca gataatattc tgaatttatt gatagataaa cagaaaaaag
    16621 tgcctttgtc agcataaaat aacggcacaa agggcggaat aattcactat cattcagggg
    16681 attatgctgg acatttttca ttctctaatg ttttaacttt gtaattattg ctgttaaaaa
    16741 attaatcacc tgccaaaaga aataaaaaag agaaagcctc cgattaaatt atttcgctac
    16801 actggttcca cttttgtgat ttacacgggt tacccatgaa gctgaccatc attcgattag
    16861 aaaactttag cgaccaggac cggattgacc tgcaaaagat ctggccggag tattcccctt
    16921 cctctttaca ggttgacgat aaccaccgta tctacgccgc gcgttttaac gagcgtctgc
    16981 ttgcggccgt gcgggtaacc ttaagcggca ccgagggagc actggattct ctacgcgtgc
    17041 gggaagtcac ccgccgtcgc ggtgtggggc aatatctgct ggaagaggtt ttgcgtaaca
    17101 atcctggcgt ttcatgctgg tggatggcgg atgcaggcgt ggaagatcgc ggtgtgatga
    17161 cggcgtttat gcaggcactg ggatttacgg cacagcaggg cggctgggag aagcgttaat
    17221 cgtcaagttg atttcaaaag tgatgaccaa cgagaaattg cctgatgcgc tacgcttatc
    17281 aggcctataa tgtgtgctgc aatttactga tttctttggt tcttgtaggc cggataaggc
    17341 gtttacgccg catccggcat gaagcaacgt attcgatatt agcaatttgg cggcaaccca
    17401 aagttgccga ttaacaatta cttcgcatcg gtcgccgtgc cgttggcgtg ccagtcaaat
    17461 acgccgaact caaagccctt tagatcgcct ttctcatccc aggtcagtgg ccccatcacg
    17521 gtatccacgg agttcgcttt taggtatttg gcgatttcag ccggatcgtc agactgattc
    17581 aggcccgcct gcaaagattg cagcgcggcg taggtggtcc agacgaatgc gccgcttggg
    17641 tcctgttttt tcgctttgat cgcatcaaca atgggtttgt tcgccggaac ctgatcgtag
    17701 ttcttcggct tggtcaccag cagcccttcc gctgattcgc ccgcaatgtt agacagcgaa
    17761 acgttagcca caccttccgg ccccataaac tgagttttca gccctgccgc gcgtgcctga
    17821 cgcaggattt gccccatttc cgggtgataa ccgccgtagt aaacgaagtc gatattctct
    17881 tttttcagac gcgccaccag cgttgagaaa tctttttccc cggcggtgat accatcaaag
    17941 aacaccacgt tagcattgcc tttcttcagg ccgtcctgca ccgctcgtgc cagaccttcg
    18001 ccgtattgct gtttgtcgtg aacgatagca atacgctgcg gtttcacttt ctcaagaata
    18061 tatttcgccg ccgtcggccc ctggtcggag tccaggccgg tggtgcgcag gatcagctga
    18121 tagccacggg cggtcagctc cggcgcggtt gccgctgggg tgatcattaa aatgccttcg
    18181 tcttcgtaga tgtcagacgc aggctgcgtt gatgaagaac agaggtgacc aatcacatat
    18241 ttaatgccgt cgttaacgac tttgttcgcc accgcaaccg cctgtttcgg gtcacaggca
    18301 tcgtcatatt ttacgatttg cagtttgttg cctttaatgc cgcctttagc gttgatatcc
    18361 gcaaccgcct gctctgcgcc ggtaaactcc tggtcaccgt actgcgcaac cggaccggac
    18421 attgcaccca cgaccgcgac tttaatatct tctgccagag ccatattgct gaatgccagc
    18481 gcgatacatc ctgccagtaa cgctttaccc tttatgttca tcctgagaat ccccattctt
    18541 ctggttatta cgtgtgttgt gatgttgttg ttcagcactt tatttcgttt tatgcatgac
    18601 tacccgtgct ttagcagcat actctgctaa aacatacccg atttttatga tattggaata
    18661 gctattttga cagtttatta acaatctgcg tggggattgg cgttttgccg gagggggaat
    18721 tgattattgt agaggcccaa aaaacaaaaa ccccggactc tcatccaggg ttctctgctt
    18781 aatagcggaa attacgcttc aatagcggcg cgaagttttt tcatcgcgtt cttttccagt
    18841 tggcgcacac gttcagcgga aacaccgtaa cggtcagcca gttcctgcaa cgtggacttg
    18901 ttgtcttcgt ccagccagcg cgcacggatg atgtcctggc tgcgttcgtc cagaccatgc
    18961 atcgcgtcgg tcagacggtt tgccgcctgc tcttcccagt tatcatcttc gatgccgtcg
    19021 gcaaagttag atgatttatc ctgcagatag agcaccggtg ccatcggctg gctgtcggaa
    19081 tcgtcgtcgg aagacaggtc aaaggtcatg tcctgtgccg ccatacgtga ttccatctca
    19141 cgtacgtctt tgctggttac gcccagttca cgggccacca tttcgacttc atcctggtta
    19201 aaccagccca gacgctgctt ggttttacgc aggttgaaga acagtttgcg ctgcgctttg
    19261 gtggtcgcaa ctttgacgat acgccagtta cgcagaacgt attcgtggat ctctgctttg
    19321 atccagtgga cggcgaagga gaccaggcgc acacccactt ccgggttaaa acggcgcact
    19381 gctttcatca ggccgatgtt accttcctga atcaaatccg cctgtggcag gccatagccc
    19441 gcataattac gagcaatatg aacaacaaac cgcaggtgag acaggatcag cgttttagct
    19501 gcttccagat cgccatggta atgcagcttt tcagccagcg cccgctcctc gtcagccgac
    19561 aacatcggcc acgcgttagc tgcccggatg taggaatcca ggttgccaac tggggctaaa
    19621 gctaaacttt gcattttgtc agtcattcaa atcctctcaa tcgatatctt ctggcgcttc
    19681 agtggtagca acaactgtgc caaagcttaa gagcaacgag gttatcattc actgttttat
    19741 cagaccgtga ttttatccac aagttcaatg caagcttgtg aataaattac gcacaaaatg
    19801 tgacatagag atgaaatacc gggaagagac aacggggtct ctttccctgc tacggaaccc
    19861 attgcaggga aagagtataa cacgctttta ttattcaggc gtaaagtggc gtaaatgttg
    19921 taccgtggca agccacgctg ccacccagcc aatcatcgag cataccagca gcaatagcag
    19981 gcattcatcg aacgataaac cattgatatc aaacttcgtt ccgaaaacct gtgccacttc
    20041 cgccaccgct gacgacaaac gcagtaccag aatttctgac aaaattaatg acaacaatgc
    20101 gccagaaaat cccagcagcg caccgccgta caggaacggg cgaaggatga aaccatccgt
    20161 cgcaccaatc agtttctgca cgttgatgga gtcacggcga gcaaagatgc tcagacgtac
    20221 gctgttgccg atgacgagga acactgccgc caccatcaac acgccgatca tcgccgaaac
    20281 gcgcccaacc agcccggtca acgccgccag acgggcaaac cagctgtcat ccatccgcac
    20341 ttcgtcaata ccgttaatct gcgtgatacg atcacgcagc gtattgagtg attccgtccc
    20401 ctgaaaatcg agtttcggga tcaccaccgc cactgccgga agcgggtttt cttccagcat
    20461 atccagcgca ccaccaaaac cagaccagtt acggaactcg cccagtgcgt cttcacgaga
    20521 aagatagttc actttctcca cgccttgctc ggcctgcaac tgtgccacca cgcccgcagc
    20581 ggcgtcatcg tccagcgttt tttgcaagta aacagtgatt tgcggtgacg gataatactg
    20641 cgtcgccgcc tgattaacgt ttttgtacac catataacag acgctgggca gcgtcaggga
    20701 gatggcgata accatcaccg ttaaaaacgt ggcgaacggt ttgcttttca gatcctgcaa
    20761 tgcgccgtgg aaggcatagc gcacctgttc gttgaaaacg ttggttttgc gatttaccgg
    20821 ttttggcgag gatttcgcgc gtttcggtgc gttacgaccg ccgtcgcctg agccgccgac
    20881 cgatttacgg aagcgatcca gacgcccacc aaactgccga atatgattga ttgcatcgcg
    20941 cttattcatg gcccacgcct ccatgcaagt gaccatcgct cagggtgagc atgcgatagg
    21001 aacgccgtga aatcagatta atgtcgtgcg ttgccatcaa tacggttacc ccaacgcggt
    21061 taaactcttc aaacagacgt agaatccctt ccgacaacgc gtcgtccagg ttaccggtcg
    21121 gttcgtccgc cagcagtacc gcgggcttat tcaccaccgc gcgggcaatg ccaacacgct
    21181 gttgttcacc gccggaaagc tgaataggga agttcttcgc tttgtccagt agcccgactt
    21241 tatccagtgc cgccgacacc cggcgacgaa tatcgtcacc gctggcacca gcgataatca
    21301 gcgggatcgc cacgttatcg tagacagtac ggtccatcag tagatggtga tcctggaaaa
    21361 tcatgccaat ctggcggcgc agaaacggaa cttcacggtt tttcagacgc gtgatgtcat
    21421 ggccgctaaa ccagattttc ccggcgctgg gccgctcaat cccacagatc agcttcagga
    21481 gggtactttt ccctgcgccg gaatgaccgg tcagaaacgc catctcaccc ggctgcatat
    21541 ggaacgtaac gccctgcagc gcctgtctcc caccgagata agccttgctg acatgttcaa
    21601 agcgaatcat tgttaatcct ctcgggcaaa aagtgcctct ataaagtcgt ccgccttaaa
    21661 cggacgcaaa tcctcaatac gttcgccgac accaatgtag cggataggga taccaaactg
    21721 gtcagccacc gagaaaatta ccccgccttt cgccgtgccg tccagtttcg ttagcgtgat
    21781 gccggttaag ccaacagctt cgtggaacag tttggcctgg cttaccgcgt tctgcccggt
    21841 gctggcatca atagtcagca taacttcatg cggcgcttca acgtcgagtt tcttcatcac
    21901 gcggacgatt ttcttcaact cttccatcag atgcgatttg ttctgcagac gtccggcggt
    21961 atcggcaatc aggacgtcga tattacgcgc tttagctgcc tgaatggcgt cgaagataac
    22021 agaggcggaa tccgcaccgg tatgctgggc aatcaccgga atattgttgc gctgacccca
    22081 gacctgaagc tgttcaaccg cagctgcacg gaaagtatcg cccgccgcca gcatcaccga
    22141 tttaccctgc tgctcaaact gacgcgccag cttaccaatc gtcgtggttt tacccacacc
    22201 gttgacgccc accatcagga tcacgaacgg cgttttatct tcaacattca gcggctcatc
    22261 gactttcgcc agaatctcgc ccatctcttc tttcagcagg ccatagagcg cctcggcatc
    22321 acgaagctgc ttgcgggatg cgccttccgt cagattggtg ataattttac gtgtggtttc
    22381 cacgcccaca tcggcgatca acagctgttc ttccagctct tcaaacagat catcgtcgat
    22441 ttttttaccg cggaacaggc tgataaatcc ggaaccgaga ttttctttgg tttttaacag
    22501 gctgcgtttc aggcgcgcga agaagccttc tttggtcggt ttttcttgct cctgggcaat
    22561 ttctgcaacc ggctgctctt cttctgccgg aggaactacc atcaccgcct cttctgccgc
    22621 ttcggcagcc agcgcctgtg cttccagctc ttcgtcggtg atttcttctt tagccgcttc
    22681 ttcttcttct gccgcttcga caatctctac ggtttccgct tcagcctgcc actcttctga
    22741 cgaaacctct tcggcgttga cgtcttccgg caacggcagc tcttcacgtt cgatagccac
    22801 tggctccggc gtttcttcta cgaccggttc cggctgtgca acaacttccg cttcaggctg
    22861 cgctttttca ctttcaacaa cctgttcagt gacttccaca acgtcggcag caaaagtttc
    22921 cgcctcggct tcagtatgcg cctgcggctg ctcttcaacg gcttgttcag aagccttcgc
    22981 aggctcttgc gcctgaacga tttcttctac aaccggttgt tcattctgaa cttctgtctc
    23041 tttttccggg gtctgctctt tttgaccaaa gcccagccag gaaaaaaagc cacgtctttt
    23101 ttctttcgcc atttgcgact acactcctcg ctgttgcttc atggcacagc gttaacgcta
    23161 tgtacatagc agctaaaaaa atgatgaaat agtctatcac ttaacttaat tcacatcacc
    23221 gcctgcaagt atgtgttatc tggcggattg agcaatttat catgaaaaaa ccgaatcatt
    23281 ccggcagcgg ccaaatccgc attattggcg ggcagtggcg aggccgtaaa ctcccggttc
    23341 ctgatagccc aggtctgcgc cccaccaccg accgcgtacg cgaaacgttg tttaactggc
    23401 tggctccggt cattgttgac gcccaatgtc tggattgctt cgccgggagc ggcgcgctgg
    23461 ggctggaagc gttatcgcgc tacgctgcgg gggcaacgtt gattgagatg gatcgcgtgg
    23521 tttctcagca gttaattaag aatctggcga cactaaaagc aggcaatgca cgcgtggtga
    23581 acagcaacgc aatgtcattc ctggcgcaaa aaggcacacc gcataatatc gtgtttgtcg
    23641 atccaccgtt ccgccgtggc ttgttagaag agacgataaa tttactggaa gataacggct
    23701 ggctggctga cgaagccctg atttatgtcg aaagcgaagt cgaaaacggt ctgcccactg
    23761 ttccggcaaa ctggtcatta catcgggaaa aagtggcggg tcaggtggct tatcggctgt
    23821 atcaacacga agcacaagga gaaagtgatg ctgattaata ttggtcgttt gttaatgctc
    23881 tgcgtttggg gatttttaat cctcaatctg gtgcatccct tcccgcgccc gctaaatatt
    23941 ttcgttaacg tggcgctgat ttttaccgtg ctgatgcatg gtatgcagct ggcgctattg
    24001 aaatccactt taccgaaaga tggcccgcag atgaccaccg ccgaaaaggt gcggattttc
    24061 cttttcggcg tgtttgaact gctggtctgg cagaagaaat ttaaagttaa aaaataacct
    24121 tattgttcgc caacaaagct aacaaagcgc gttcctttat agctcagcgt acctttatcg
    24181 cccactgtca gggcgtggta ctgctgggcg tcgaggcgaa acgtctgctc cattcctccg
    24241 ctttgcggtt tgaagcttgc ctcatagcgc atactggtgc ctgccggagt cacttcctgc
    24301 tggcgcgaac ggcgatcgtt aatcggtttt tcccgcttgt tgctcaccac caccagcttt
    24361 tgctggagcg gagccatatc attatcagct ttttcccgtc gctgttgcat aaaacgaaac
    24421 gatgcggcga cgacaattaa gccaatgata acaataaaga aaagaggtgg tttactcatc
    24481 tttatccctc atcggaaaat gcggaattaa gcataccctg ccagttatgg tgttgtcatc
    24541 cgtccaccct cgccactaaa ctggaagcaa gaccgtaggc attctgctta cgaaaaaata
    24601 acgaattcaa ggaactaaga tgctttggtc gtttatcgct gtctgtcttt ccgcatggct
    24661 atctgtggat gcatcgtatc gtgggccaac ctggcaacgc tgggtgttta aaccgttaac
    24721 ccttcttctc ctgctgttac tggcctggca agcaccgatg ttcgacgcca ttagctatct
    24781 ggtgctggcg ggactgtgcg cctcactgct gggcgatgcg ctaaccctgt tgccacgtca
    24841 acgtctgatg tacgccatcg gcgcgttttt cctctcgcac ctgctgtaca ccatctattt
    24901 cgccagtcag atgacgctct ctttcttctg gcctctgcca ctggtgttgc tggttctggg
    24961 tgcgctgcta ctggcgatta tctggacgcg gctggaagag taccgttggc ctatctgcac
    25021 gtttatcggc atgacgctgg tgatggtgtg gctggcaggt gaactgtggt tcttccgtcc
    25081 gaccgctccg gcgctctctg cgtttgttgg cgcttcgttg ctgtttatca gtaactttgt
    25141 ctggctgggg agccactatc gccgacgctt ccgtgcggat aacgcgattg ctgcggcctg
    25201 ctactttgcc ggtcacttcc tcatcgtccg ctcactgtat ctctgataaa acttgactct
    25261 ggagtcgact ccagagtgta tccttcggtt aatgagaaaa aacttaaccg gaggatgcca
    25321 tgtcgacacc tgacaatcac ggcaagaaag cccctcaatt tgctgcgttc aaaccgctaa
    25381 ccacggtaca gaacgccaac gactgttgct gcgacggcgc atgctccagc tcgccaacgc
    25441 tctctgaaaa cgtctccggc acccgctata gctggaaagt cagcggcatg gactgcgccg
    25501 cctgtgcgcg caaggtagaa aatgccgtgc gccagcttgc aggcgtgaat caggtgcagg
    25561 tgttgttcgc caccgaaaaa ctggtggttg atgccgacaa tgacatccgt gcccaggttg
    25621 aatctgcggt gcaaaaagcg ggctattccc tgcgcgatga gcaggccgcc gacgagccac
    25681 aagcatcgcg cctgaaagag aatctgccgc tgattacgct tatcgtgatg atggcaatca
    25741 gctggggtct ggagcagttc aatcatccgt tcgggcaact ggcgtttatc gcgaccacgc
    25801 tagttgggct gtacccgatt gctcgtcagg cattacggct gatcaaatcc ggcagctact
    25861 tcgccattga aaccttaatg agcgtagccg ctattggtgc actgtttatt ggcgcaacgg
    25921 ctgaagctgc gatggtgttg ctgctgtttt tgattggtga acgactggaa ggctgggccg
    25981 ccagccgcgc gcgtcagggc gttagcgcgt taatggcgct gaaaccagaa accgccacgc
    26041 gcctgcgtaa cggtgagcgg gaagaggtgg cgattaacag cctgcggcct ggcgatgtga
    26101 ttgaagtcgc cgcaggtggg cgtttgcctg ccgacggtaa actgctctca ccgtttgcca
    26161 gttttgatga aagcgcccta accggagaat ccattccggt ggagcgcgcg acgggcgata
    26221 aagttcctgc aggagccacc agcgtagacc gtctggtaac gctggaagtg ctgtcagaac
    26281 cgggtgccag cgccattgac cggattctga aactgatcga agaagccgaa gagcgccgcg
    26341 cccccattga gcggtttatc gaccgtttca gccgtattta tacgccagcg attatggccg
    26401 tcgccctgct ggtaacgcta gtgccgccgc tgctgtttgc cgccagctgg caggagtgga
    26461 tttataaagg tctgacgctg ctgctgattg gttgcccgtg tgcgttagtt atctcaacgc
    26521 ctgcggcgat aacctccggg ctggcagcag cagcgcgtcg tggggcgtta attaaaggcg
    26581 gtgcggcgct ggaacaacta ggtcgtgtta cccaagtggc gtttgataaa accggtacgc
    26641 tgaccgtcgg taaaccgcgc gttaccgcga ttcatccggc aacgggtatt agtgaatctg
    26701 aactgctgac actggcggcg gcggtcgagc aaggcgcgac gcatccactg gcgcaagcca
    26761 tcgtacgcga agcacaggtt gctgaactcg ccattcccac cgccgaatca cagcgggcac
    26821 tggtcgggtc tggcattgaa gcgcaggtta atggtgagcg cgtgttgata tgcgctgccg
    26881 ggaaacatcc cgctgatgca tttgctggtt tgattaatga actggaaagc gccgggcaaa
    26941 cggttgtgct ggtagtacgt aatgatgacg tgctgggtat cattgcattg caggataccc
    27001 tgcgcgccga cgctacaact gccatcagtg aactgaacgc gctgggggtt aaaggagtga
    27061 tcctcaccgg cgataatcca cgcgcagcgg cggcaattgc cggggagctg gggctggagt
    27121 ttaaagcggg cctattgccg gaagataaag tcaaagcggt gaccaagctg aatcaacatg
    27181 cgcccctggc gatggtcggt gatggtatta acgacgcgcc agcgatgaaa gctgccgcca
    27241 tcgggattgc tatgggtagc ggcacagacg tggcgctgga aaccgccgac gcagcattaa
    27301 cccataacca cctgcgcggc ctggtgcaaa tgattgaact ggcacgcgcc actcacgcca
    27361 atatccgcca gaacatcacc attgcgctgg gactgaaagg gatcttcctc gtcaccacgc
    27421 ttttagggat gaccgggttg tggctggcag tgctggcaga tacgggtgcg acggtgctgg
    27481 tgacggcgaa tgcgttaagg ttgttgcgca ggagataagg caaaccgatc gcaacattga
    27541 gcgcgatcgg tcccctcgcc cctctgggga gagggttagg gggtgaggaa aaggcggcat
    27601 cgaagccaat cagcccctat caaccgcctt tacgaatcaa ataacgataa ggcagtccat
    27661 ccgtctcttt cgcaaccagt tcgtgttcca taaaggtaca aaacccagga atatcgcggg
    27721 tagtggccgg atcgtcggcg ataatcagca acgtttcgcc aggctgcata ttgcgcacgg
    27781 ttttgcgcac catcatcacc ggttccgggc agcgcaggcc aagcgcgtcg agtgtgtggt
    27841 cagggctgga aaagagatcg gtcattttct tctcatcact taaaaaaacg gcgctagttt
    27901 acgccctgtg agtccgtaag caaaccaggt taacgattgc gtgaaaatta accattgcat
    27961 tgtcaacgta aagcagtatc atgcggcggc tcgaaaaaag ggtaagcacg ttattatgtt
    28021 aaggtaacag acgtgtcgta cgtattgggt tccctcaccc caatggttaa tcaaaaaggt
    28081 acaatatgaa cgttttctcg caaactcaac gctataaggc gttgttctgg ttatcgttat
    28141 ttcatcttct ggtgatcacc tccagtaact atctggttca gcttcccgtc tccatttttg
    28201 gtttccatac cacctggggc gcgtttagct ttccgtttat ttttcttgct accgacctga
    28261 ccgtgcgtat ttttggcgca ccgctggccc gacgcattat cttcgcggta atgatccctg
    28321 cgttattaat ctcctacgtc atctcgtcgc tattctatat gggttcctgg cagggattcg
    28381 gcgcactcgc ccacttcaac ctgtttgtcg cccgtatcgc caccgccagt ttcatggcct
    28441 acgcgctggg gcaaatcctc gacgtgcacg tttttaaccg cctgcgtcag agtcgccgct
    28501 ggtggctggc accgacagcg tccacactgt tcggtaacgt cagcgacacg ctggcctttt
    28561 tcttcattgc cttctggcgt agcccggatg cctttatggc tgaacactgg atggaaatcg
    28621 cgctggtcga ttactgtttc aaagtgttaa tcagtatcgt tttcttcctg ccaatgtatg
    28681 gcgtattact caatatgctg ttgaaaagac tggcagataa atccgaaatc aacgctttgc
    28741 aggcgagtta aaggttcgtt atcagagttg tgataagatg gatgaatgag ccgttatggc
    28801 cgtttatcga aaggaagaag tcaatgcgca atctggttaa atatgtcgga attggcctgc
    28861 tggttatggg gcttgcggcc tgtgatgata aagacactaa cgctacggcg cagggttcgg
    28921 ttgcggaaag taacgctacc gggaatcccg tcaacctgct tgatggcaag ttaagtttct
    28981 cgctgccagc ggatatgacc gaccagagcg gtaagctggg aacgcaggcc aataacatgc
    29041 atgtctggtc cgacgccacc gggcagaaag cagtcatcgt catcatgggc gatgatccga
    29101 aagaagatct ggcggtgttg gcgaagcgtc tggaagatca gcaacgtagc cgcgatccgc
    29161 agctgcaagt ggtaaccaat aaagccattg agctgaaagg tcacaaaatg cagcagttag
    29221 acagtattat ctccgcgaaa ggccagacgg cgtactcttc cgttattctg ggtaacgtgg
    29281 gtaatcaact gctgaccatg caaattacgc tgccagctga cgatcagcaa aaagcgcaaa
    29341 ccaccgcaga aaacatcatt aatacgctgg ttattcagta agttttaaga tgatgaggcg
    29401 gcctcaggga cgtgttccgg aggccgtttt tttaatcgcc acgtcagtaa taacgcgatt
    29461 gcgaccagtc ccgccgccgc cagataaatc accggtacgc ccgcccagct catcaccagc
    29521 ccagccagtg gtccagtcac gccaagcgat aaatccataa atacggtgta agttgccagc
    29581 gccgccccct gattttgctg cggaaccgct tttaccgcca ctacacccaa tgccgggaac
    29641 accagcgaaa acccggcccc cgccagtaag acgccgattt tcgccatcca cggcatagtc
    29701 gccacgccaa ccagtagcag gccgattatc tcaacgctaa agcaaatcat cgctacgttt
    29761 aagccaccga tacggttaat gccgttaggg aataacaaac gcgtaccgac aaacgcacag
    29821 ctaaacagcg tcagcgcgaa agccgcaccg tcccaacctt tagcgtcata aaacagcgtg
    29881 ataaaggtgg cgatgacgcc aaatccggcg gaagccagtg ccagcgccat accgtacagc
    29941 cagacgcgcc caagcaccgc gcgaaacggc agcggtttgc ctttactggc ttttaccgtc
    30001 ggacgcggga tcgccaacaa aatggccacc agcgccacgc ccataatgat taacgctaac
    30061 gcctgcaagc cgccccagtg ataaaacacg acgcctaacg gcgcacccat cgccatcgcc
    30121 ccgtaagtga caatgccgtt ccacgaaatc acccgcccga tatgcagcga gccaaccact
    30181 ccaacgcccc atagggtcga tcccgttccg gcaaaacttt gcccaatccc aaggatgacg
    30241 cgccccaggc aaagtaataa caggctgatg acaggcagac tggcggttaa tcctgccgtc
    30301 agatacccca gaccgctcaa aaagcagccg cataaaccga agacgacaat ctttttgggt
    30361 cccagcaaat cggcgtaacg tccggcatga gggcggctca gcaaggtggc gaaatattgc
    30421 aggctgataa ccaatcctgc ccagaaggcg ctaaagccca tcacatcatg gacatagccc
    30481 ggtaatacag cgagcggcaa cccgatggtg aggtagctgg cgaagttaaa catgacaatg
    30541 gagacaatgc gcaaattcag gcgcaatccg tttagcgcgg gttcggctac gggttcgggc
    30601 atgaggatca ccacattttt acaacagtgt ttcattttta ccatgtgctg acgtgaaaat
    30661 cagcagtaag aatcagaata ttgctggcgt gactcccgcg ctacacttaa cacaaaaagt
    30721 cacaaggaag ccccaatgga aacccctcaa cccgataaaa cgggcatgca cattctgctc
    30781 aagttggcct cgctggtagt gatcctcgcg ggcattcacg cagcggcaga tatcattgtg
    30841 cagctgttac tggcgctgtt ttttgccatc gtcctcaacc cgctcgttac ctggtttatt
    30901 cgtcggggag tacaacgccc cgttgccatt acgattgtag tggtggtgat gctgatcgca
    30961 ctaaccgcgc tggtcggcgt actggcggca tcgtttaacg aatttatctc tatgctgccg
    31021 aagtttaata aggagctgac gcgcaaactt tttaaattgc aggagatgtt gccttttctt
    31081 aatttgcata tgtcgccgga gcgaatgctg cagcggatgg actcggaaaa agtggttacc
    31141 ttcaccacag cgctaatgac cgggctttcc ggggcaatgg cgagcgtgct tttgctggtg
    31201 atgaccgtag tttttatgct gtttgaagtg cgccacgtcc cttacaaaat gcgttttgcg
    31261 ctgaataacc cacagattca catcgcagga ttacaccgcg cacttaaagg cgtttcgcat
    31321 tatctggcgt taaagacgct actcagttta tggacaggtg tcatcgtctg gctggggctg
    31381 gcgctaatgg gcgtacagtt tgcgctgatg tgggcagtac tggcgttttt gctcaactac
    31441 gtgcccaata tcggcgcggt aatttccgcc gtaccgccaa tgattcaggt gctgctgttt
    31501 aatggtgttt acgaatgtat tctggtcggc gcattgtttt tagtggtcca tatggtcatc
    31561 ggcaatattt tagaaccacg gatgatgggc catcgcctgg ggatgtccac catggtggta
    31621 tttctttcat tgttaatctg gggatggctg ctcggcccgg tagggatgct actttcggtg
    31681 ccattaacca gcgtgtgtaa aatctggatg gaaacgacga aaggtggcag caaactggcg
    31741 attttactgg ggccgggcag accgaaaagt cggttaccgg gatgaggcga caagtgatac
    31801 gatacgcact ttcattttcc attaaacgtt ggccctgata tgtatcggat agttctgggg
    31861 aaagtttcga ccttaagcgc agctccactg ccaccgggtt tacgcgagca agcaccgcaa
    31921 ggtccacgac gcgaacgctg gctggcgggg cgtacattgc tttcgcacac gctttccccg
    31981 ctaccggaga tcatctatgg cgaacaaggc aaacctgcat tcgcgccgga aacgccgcta
    32041 tggttcaact taagccatag cggtgacgat atcgctctgc tgttgagcga tgaaggcgaa
    32101 gtcggctgcg atatcgaagt gattcgcccg cgcgtcaact ggcgctggct ggcgaacgcc
    32161 gtgttcagcc tcggggaaca cgctgaaatg gacgccgtgc atcctgatca gcaactggaa
    32221 atgttctggc gcatctggac gcgcaaagaa gccatcgtta aacagcgtgg cggcagcgcc
    32281 tggcaaatcg tcagcgtaga tagcacctat cactcctcgc tgtcagtcag ccattgtcag
    32341 ctagaaaatt taagccttgc gatctgcacc cccaccccct ttacgctcac cgccgacagt
    32401 gtgcaatgga tcgattcagt taactgatcc gcccacccga ctgcccatct attgatccag
    32461 aacaggtaat cagtatgacg aatacttaaa atcgtcatac ttatttccgc catctatttt
    32521 aatccattgg ggttaccatg ctctccacac tccgccgcac tctatttgcg ctgctggctt
    32581 gtgcgtcttt tatcgtccat gccgctgcac cagatgaaat caccaccgcc tggccggtga
    32641 atgtcgggcc actaaacccg cacctttaca cgcctaacca gatgttcgcc cagagcatgg
    32701 tttatgaacc attggtgaaa tatcaggcag acggttcggt gatcccgtgg ctggcaaaaa
    32761 gctggactca ttcagaagat ggtaaaacct ggaccttcac cctgcgtgat gacgtgaaat
    32821 tctccaacgg tgaaccgttc gatgccgagg cggcggcaga aaacttccgc gcagtgctcg
    32881 ataaccgtca acgtcacgcc tggctggagc tggcaaacca gattgttgat gttaaagcac
    32941 tcaataaaac agagctgcaa attaccctga aaagcgccta ctatcctttc ctgcaagaac
    33001 tggccctgcc ccgtcctttc cgttttatcg ctccctcgca gtttaaaaac catgaaacca
    33061 tgaacggaat taaagcgccg attggcaccg gaccgtggat tttgcaggaa tcgaaactaa
    33121 atcagtacga tgtcttcgtc cgtaacgaaa actactgggg cgaaaagcca gcgattaaaa
    33181 agatcacctt taacgtcatc ccggacccga ctacccgcgc ggtggcgttt gaaactggcg
    33241 atatcgacct gctgtacgga aacgaagggt tattaccgct cgataccttc gcccgcttta
    33301 gccagaatcc ggcttaccac acccaactgt cacagccgat cgaaaccgtg atgctggcgc
    33361 tcaataccgc caaagccccc accaacgagc tggcagtacg tgaagctctt aattacgcgg
    33421 taaacaaaaa atcgctgatt gataacgcgt tatatggcac ccagcaggtc gccgacaccc
    33481 tgtttgcccc ttctgtgcct tacgctaatc tcggcctgaa accgcgccag tacgatccgc
    33541 agaaagcgaa agagttgctg gaaaaagccg gttggacgct gcctgcgggc aaagacatcc
    33601 gcgagaaaaa tggtcagccg ctgcgcattg aactgtcgtt catcggcacc gatgcgttaa
    33661 gcaaatcgat ggcggaaatc attcaggctg atatgcgcca gattggcgca gatgtctcgc
    33721 tgattggcga agaagagagc agtatctatg ctcgtcagcg cgacggtcgt tttggcatga
    33781 ttttccaccg cacctggggc gcgccatacg atccacacgc cttcctgagt tcaatgcgcg
    33841 taccgtcgca cgctgacttc caggcacagc aaggattagc cgacaagccg ctgattgata
    33901 aagagatcgg cgaagtgctg gcgacccatg acgaaacgca acgtcaggcg ctgtatcgcg
    33961 acattctgac ccgtctgcat gacgaggcgg tttatctgcc tatcagctac atctcgatga
    34021 tggtggtatc aaaaccggag ctgggtaata tcccctacgc gccgatcgcc accgaaattc
    34081 cgttcgaaca gattaaaccg gtgaaacctt aatgttgcgt tacgtattac gccgctttct
    34141 gctgctgatc ccgatggtgc tcgccgcctc ggtgatcatt tttctgatgc tgcgcctcgg
    34201 taccggcgac ccggcgctcg attatctgcg tttatctaac ctgccgccga cgccggagat
    34261 gctggcctct acccgcacca tgctcgggct ggatcagccg ctgtacgtcc agtacggtac
    34321 ctggttgtgg aaggcgctgc atcttgactt tggtatctca ttcgccagcc aacgcccggt
    34381 actggacgat atgctgaact tcctgcccgc cacgctggaa cttgcaggtg cggcgctggt
    34441 attaattctg ctcacttccg taccgctcgg tatctgggcg gcgcgccacc gcgatcgttt
    34501 gccggatttc gccgtgcgct ttatcgcgtt tcttggcgtg tcgatgccca acttctggct
    34561 ggcatttttg ctagtgatgg cgttttcggt gtatctgcaa tggctacccg cgatgggcta
    34621 cggcagctgg cagcacatca ttttacctgc ggtttccatc gcctttatgt cgctggcgat
    34681 taacgcgcga ctgctgcgcg ccagtatgct ggacgtcgcc ggtcagcgtc acgtcacctg
    34741 ggcgcggcta cgcggcctga atgacaaaca gaccgaacgc cgccatattc tgcgcaatgc
    34801 ctcgctgccg atgatcaccg ccgtggggat gcacattggc gaattgattg gcgggacgat
    34861 gattatcgaa aacatctttg cctggcctgg cgtcgggcgc tatgcggtgt cggcgatttt
    34921 taaccgtgac tatccggtga ttcagtgctt tacgctgatg atggtggtgg tttttgtggt
    34981 ctgtaatttg attgtcgatt tgctcaacgc cgcgctggac ccgcgcattc gtcgtcatga
    35041 aggagcgcac gcgtgaactt tttcctctct tcccgctggt cggtacgcct ggcgctgatc
    35101 attatcgccc tgctggcgct gattgcgctc accagccagt ggtggcagcc gtacgatcca
    35161 caggcaattg atttgccgtc gtgcctgctt tcgccggatg cgcagcactg gctgggcacc
    35221 gatcacttag gtcgcgatat tttctcgcgg ctgatggcag cgacccgcgt gtcgctcggt
    35281 tcggtaatgg cctgcctgct gctggtgctg acattagggc tggttattgg cggcagcgcc
    35341 gggttgattg gcgggcacgt tgatcaggcc accatgcgcg tcgccgatat gtttatgacc
    35401 ttcccgacct cgattctgtc gttctttatg gttggcgtgc tcggcaccgg gctgaccaac
    35461 gtaattatcg ccatcgccct gtcgcactgg gcgtggtatg cacgcatggt gcgcagcttg
    35521 gtgatttcac tacgccaacg cgagtttgtg ctggcgtcac ggctttccgg tgcgggccat
    35581 gtgcgggtgt ttgtcgatca tctggcaggc gcggtgatcc cttcgctgct ggtgctggca
    35641 acgctggata tcggccatat gatgctgcac gtcgcgggga tgtctttcct tggcctcggt
    35701 gtgaccgcgc cgaccgccga atggggcgtg atgattaacg acgcgcgcca gtatatctgg
    35761 acccagccgc tgcaaatgtt ctggccgggg ctggcgctgt ttatcagcgt gatggccttt
    35821 aacctggtgg gtgacgcact gcgcgatcat ctggatcctc atctggtgac ggagcacgca
    35881 cactaatgcc gcaacagatt gaactacgta atatcacgct acaggccgcg cagccgctgg
    35941 tacacggtgt atcgttaacc ctgcaacgcg ggcgcgtgct ggcgttagtc ggcggtagcg
    36001 gcagcgggaa atcattaacc tgcgccgcga cgctgggcat tttgcccgct ggcgttcgcc
    36061 agacggcggg ggaaatttta gccgatggca aaccggtttc gccttacgcc ctgcgcggca
    36121 tcaaaattgc caccatcatg cagaacccgc gcagcgcctt taatccactg cacaccatgc
    36181 acacccacgc gcgggaaacc tgcctggcgt tagggaaacc cgccgatgac gctacgctta
    36241 ccgctgccat agaagcggtg gggctggaaa acgccgcgcg cgtgctgaag ctgtacccgt
    36301 tcgagatgag cggcggcatg ttgcagcgca tgatgattgc gatggcggtg ctgtgtgaat
    36361 caccgtttat catcgccgat gaaccgacca ccgacctcga cgtggtagca caggcgcgca
    36421 tcctcgatct gctggaaagc attatgcaaa aacaagcgcc gggaatgctg ctggtcaccc
    36481 atgatatggg cgttgtggcg cgtctggcgg atgacgtggc ggtgatgtca caaggtaaaa
    36541 ttgtcgaaca gggcgatgta gaaacgctgt ttaacgcccc caaacatacg gtgacgcgca
    36601 gcctggtttc cgctcatctc gccctctacg gtatggagct ggcatcatga ccttacttaa
    36661 cgtctgcggc ctttcccatc actatgccca cggtggattt agcggaaaac atcaacatca
    36721 ggcggtgctg aataacgttt ccctgaccct gaaaagcggc gaaaccgtcg ccctgctggg
    36781 acgcagcggc tgcggaaaaa gcaccctcgc gcggctgctg gtaggcttag agtctccctc
    36841 acaagggaat attagctggc acggcgaatc gctggcgaaa cttaatcgcg cccaacgtaa
    36901 agccttccgc cgtgatattc agatggtgtt tcaggactcc atcagtgccg tgaatccgcg
    36961 caaaaccgtg cgcgagatcc tgcgtgaacc gatgcgccac ctgctatcac tgaaaaaatc
    37021 cgaacaactg gcgcgcgcca gcgaaatgct gaaggcggtc gatctcgatg acagcgttct
    37081 cgacaaacgc ccaccgcagt taagcggcgg ccagctccag ctcgtctgcc tggcacgcgc
    37141 gctggcggtc gaaccgaaat tgctgattct ggatgaagcc gtttctaacc ttgatctcgt
    37201 gttacaggcg ggcgtcattc gcctgctgaa aaagctacaa caacagtttg gcactgcctg
    37261 cctgttcatc actcatgact tacgcctggt ggaacgcttt tgccagcgag tgatggtgat
    37321 ggacaacgga caaatcgccg aaacccaggc ggtgggagat aaattaacct tttcctctga
    37381 cgccggacgt gtgctacaaa acgcggtatt acccgcattc cccgtgcgcc gtcgcacctc
    37441 agaaaaggtt taacgccaat gcaacgagtc accatcacgc ttgatgacga tttactggag
    37501 acgctggaca gcctgagcca gcgtcgtggt tataacaacc gttccgaagc tatccgcgat
    37561 attctgcgta gtgccctggc gcaggaagct acccagcagc acggcacaca aggtttcgcg
    37621 gtgctgtcgt atgtgtatga acacgaaaaa cgcgacttag ccagccgtat tgtttccacc
    37681 cagcatcatc accacgacct ctccgtcgcc acgctacatg tgcacatcaa ccacgacgac
    37741 tgtctggaaa tcgccgtgtt gaaaggtgac atgggtgacg tgcagcattt tgccgatgac
    37801 gttatcgccc agcgcggcgt gcggcacggg catttgcagt gcctgccgaa ggaagattga
    37861 gttatttcta aaacaaggaa agaaaagata ttaactgaac ttaatttata aaattaagtt
    37921 cagttgcata gatagatcct gtaaaggcag atattctgcg taatgtacgt cgctaaaatt
    37981 tataaatgat tcaaacgctc attcagtgtt tcaataatat aggtattaac agagacttgt
    38041 tgctgcgcag ccgcattatt aaggcgttca ctcagcgatt ctggatagcg taacgtaaag
    38101 gtcttaatct tttctgtacg cgcatacggc tcaatacccg cagctttaca atcctcaaga
    38161 tactctcgca gggaaagctc tccttctttt tgcagcccct gaatactgtc agagacaaaa
    38221 tcacaataac ctgacaagcc caggaatttg ccacgaaatg cattcagttc tggcacgtag
    38281 gtaataacag ctggttgccc ggcaatctcc atagagtttg gcgtcttcag tttgatcatg
    38341 gttttacccc tatacttagt aaccagtcac acacgctttc tacagcgcct ttatcggtat
    38401 ctggcgacgg atgaggccga tggaaacacg caacgctcat atttagtatg aacttgcaac
    38461 gcgaacctct tccttcctta atttctccgc ctaatgcttt aaccagtgac tctatatcag
    38521 accacttgat cccttgagga acaggagttt taaatatttg atccaatgta tttttttgtt
    38581 tcttccgtaa agcctgtacc ttggttctca tggcatgtcc ttatgacttc actttatgac
    38641 atcacagtat gaagtcaagt ttcccttcct tacgccattg tcccaatcgt cttcctgaat
    38701 cgcagcagtg cgatggtgaa aaacgcgccg ccaattgcca tcagcgtcag gaactgcggc
    38761 cagacgattt cgaatccggc accccggtag aggatggcct gcgcgaggct aacaaagtgt
    38821 gtcgtcggca tggtcagcat aatgtcctgc accatctgcg gcatactttc gcgcggcgtg
    38881 gatccaccgg aaagcatttg cagcggcagc agcaccagaa tcaccagcag ccccagttgc
    38941 ggcattgaac gcgctatcgt ccccataaaa atgccgattg acgtggtggc aaacagactg
    39001 agcgccacgc ccagcataaa cagcgggatc gagccttcaa tcggtacgcc cagtacacct
    39061 ttcaccatca gcaccagcga taatcccgat accaccagca ccaccagccc catcgaccag
    39121 atcttcgcca tcatgatctc aaacggcgtt atcggcatca ccagtaaatg ttccaccgtg
    39181 ccgtgttcac gctcgcggat cagcgccgat ccggtcaata caatcgccag catggtaatg
    39241 ttgttgatga tcgccatcac cccgccaaac cacgcgggat caaggttcgg gttaaagcgc
    39301 atccgggttt ccagcgatac caacggttcg ctgttatcac ggtagcgcgc gacaaagctg
    39361 ttcacttcac cgttgataat attctggata tacccattgc cggtaaatgc ctggctcatg
    39421 cgcgtggcat cgacgttcac ctgaatatcc ggctggcgtc cggcgaggac atcacgctga
    39481 aaattaggcg gaatatttat cgcgaaggta tagcgtccgg cgtccagtcc ggcatccatc
    39541 tcatcggcgg tgatcatctc cggtggcaaa aaccacggac gatagaagct gttaacgatc
    39601 cggttcgata actgcgattg atccatatcg gcaatggcga tcggcgcgag gttcaacgat
    39661 cctggcgtaa cggtcgctga cgaatacacc gacaccgtaa acgagaagac aatcagcgtc
    39721 agcatcgctt tatcaccgag cagactgcgc aactctttga tacccagatt aaaaatattg
    39781 cgtaaatggc gcatcatccc tcctgttttt tcagcagcag gatgcttaag cccatcacca
    39841 gcgggatggc tatcagcaac gggataaaaa gttgccacaa atcagtcaga tccagcgctt
    39901 tcgagaacgt tccgcgggcg atagtcagaa aatgactggt cgggtaaacc tcgccgatcc
    39961 aacgtccagg cccttccagc gaagctaccg gatcgatcat cccggaaaac tgtgtcgccg
    40021 ggatcaatgt gataatcgcc gttccgaaaa tggcggcaat ctggcttttc ataaaggtgg
    40081 agatcagcag ccccattcca gtggcaatga tgatatacag cagcgccgcc aaggtgagcg
    40141 tcaggaaact gcctttatgc ggtacgccaa acacaaacac cgacaggccg cagagcagga
    40201 aaaagttcag catccccagc gcgatgtacg gcagctgttt gccaagcaaa aattcgctac
    40261 gggtggtggg ggtcacgtaa aggttgataa tcgaccctag ctctttttcc cgcaccacgc
    40321 taagggcgct tagcattgac gggatcatca tcagcagaag cgggatcacc gccggaacaa
    40381 tcgctggcag gctttttacg tccgggttat agcgataacg cgtctcaatg ttcatcagcc
    40441 cgctttggct ggcgggtgtc gattgtcggc tcgccacatc ctgaagccaa ctctggtgca
    40501 ttgcctgcac gtaacctttt accgtttcgg cacgactggg catcgctccg tcgatccaga
    40561 cgccgagttc cacaggcgta ccacgcgcga tatcgcgccc gaaattgggc gggatctcaa
    40621 tggccaccgt gatatcgccc gcacgcatcc gacgatcaag ctcgtcataa ctggtgagcg
    40681 gcggctgttc gataaagtaa cgggaaccgg agaggttgag tgtccacgcc tggctactga
    40741 cggtctggtc gcggtcgagc accgcaaagc gcaggttttc cacatccata ctgatgccgt
    40801 aacccattat cagcatcagg atcaccgttc ccatcagcgc cagcgtcgaa cgtactggat
    40861 cgcgtcgcag ttccagcgct tcgcggcggc tgtagctaaa cagacggcgc aggctaaatc
    40921 cctgacgcgg cgcgtgggtg gtgtcgtgta ccacgggcgg cgcttcggct tcgttgctct
    40981 gccctgccgc ttcctgcaaa taggcgataa atgcctcttc cagactggcg gctccgcgtt
    41041 tctcaaccag ttcctgcggt gtaccgctgg caagcacttt tccggcgtgc atcagtgaga
    41101 tgcggtcgca acgttccgct tcgttcataa agtgggtgga gatgaagata gtcactttgt
    41161 cctggcgcga gagatcgacc atcaactgcc agaacatatc cctcgccacc ggatcgacac
    41221 cagaagtagg ctcatcgagg attaacatct ccgggcgatg aatcaccgcc accgccagcg
    41281 aaagccgctg gcgaatgccg agcggcaatg actccggcag aacatcttca acgtcgttga
    41341 gcttaaaacg ctcgctcatt tcagccactc gcgcgggaat ttccgcttcc gggatgtgaa
    41401 acaaacgggc atgtaactcc aggttttgcc gcacggtgag ttcgttatag agcgaaaacg
    41461 cctgcgacat atagcccaca cgacggcggg tatcgatatc ttttggatca accggttgcc
    41521 caaatagcca cgcctcacct tcgctggcgg gcagcagtcc ggtaagcatt ttcatggtgg
    41581 tggatttacc gcagccgttc gaaccaagga atccaaaaat ctccccgcgt ggaatgcgga
    41641 aattgacgtg atcaacggca acgaaggaac caaaacgcat ggtcagatcg cgcgcttcga
    41701 tggcaatctc tgcgttttca ggttgatacg gtgggatcac taccgcctga tgcgcctggc
    41761 gttgcgcttg cggtaacaga ttaataaatg cttcttccag cgtggcgctt tgcgtttgct
    41821 gccgcagctc ttcggcgctg ccggttgcca gcacttctcc ggcattcatc gctaccagcc
    41881 agtcgaagcg ttcggcctct tccatatagg cggtggcgac cagcacgctc atattgctct
    41941 gccgctggcg aatactgtcg atcagatccc agaactgggc gcgggagagc gggtcaaccc
    42001 ccgttgttgg ctcatcaagg atcaacagtt ccgggtcgtg gattaacgcg cagcacagcc
    42061 caagtttttg cttcatcccg ccggagagtt tacctgccgg acgatcgcga aacggtgcta
    42121 acccggtgct ggtcagcagc tcattaattc gcgcttcccg ctccgcttta tcgtgaccga
    42181 acaggcgagc gaaaaaatcg acattttcat acaccgacaa ggtgtggtag aggtttttgc
    42241 ccagcccctg cggcatccag gcgatgcgcg ggcagacgtc tcggcgatgc ttcgggtcgt
    42301 gcatatcgcc acccagcacc accacattgc cctgctcaat gacgcgggca ccggaaatca
    42361 gtgacaataa actcgacttc ccgacgccat ccggaccaat cagcccgacc atgcaacggg
    42421 ccggaatatc gagagtgata ttgttcagcg caacggtttt tccataatgc tggctcacgc
    42481 ccgccagttg cgcgacaggc gggacgggaa ccagttccag atgcgtcatt gcggcaacct
    42541 caccacgagg tcgtcaggcc acggaagttc ttcattcacc cgcacccacg ctacgcccgg
    42601 taaaccggtt ttgacatatt ccagatgctg ctggagtaat tccggtggga tacgcgcttt
    42661 gacgcggaac atcagtttca gccgttcatc gctggtttcg acggtttttg gcgtgaactg
    42721 ggcgacactg gcgacaaaac tgatggttgc aggaatacgc agatctggcg cggcatcgag
    42781 gatcagccgg gcttcaccgc ccagtttcag cgtgcccgcc tgttcggttg gcaggaagaa
    42841 ggtcatatag acgtcgctga gatcgaccat attcagcacc cgaccgcctg ccgccagcac
    42901 ttcccctggc tcggcaaccc gatactgcac gcgtccgtca cgcggggctt tcagttcact
    42961 gtcatcgata tctgcggcaa tgcgccgttc agtggcttgc gctgcttcga cccgagtttg
    43021 cgcctgaatg atattggtgc gtgctgcttc tatagccgct ttagaagccg aaacctgggc
    43081 tttcgctgat tccagcgcag ctcgggcgct ctcagcggcg gcgcggtcgt catccagctg
    43141 ttgcgcagaa atagcccctc gttgggcgag cgagcgggaa cgcgtatgac gtttggcaac
    43201 agagtccagt tcggcctggc gttgattgac cagcgactgt gcggcacgag tttcactttg
    43261 tcgttgctcc agcaaagcct gcgcggcggc aacggcgctt tgtgcctctt tgatttgcgc
    43321 gatggcttcc agtcgctgtt cctgcaacac gcgagtatcc atcttcgcca gcacttcgcc
    43381 ttcgcgaaca aactggcctt ctttcaccag aatggtgtcg atacgcccgg caattttgct
    43441 ggcgatatcc acttccgtcg cttcaatgcg cccattgctg acagcaaagc cttccggcac
    43501 acccgccggg cgcaacagcc accacgccac gacggcagcc accgccagtg ccccgacaac
    43561 ccaccacgcc agatggcgct tactcttatc cataatcgac ccgccataat ccctgtaagc
    43621 aaacgacgcg tgttacaact gccgccgaac gacgttcagc ggattcttcc atccgggata
    43681 agaaggaaac gacaaatacg accagcgcgc agagcattga gcgccaacgg ggaaggctta
    43741 gtgaaaacgg gtagtaatgt tgtcatcatc agcattcctg gccgtaaatg aaacacgcat
    43801 actcataagt gtgatctata aaactggctg acaaaacggt atccatcctg ttggatcgca
    43861 tttcacgcct gttcagttgc aggaacagag cattcctttt tacgttttat caacagtctc
    43921 aaccagatag taacattaat attcatgttg tctatggttc agagctgtaa cattgtgcgc
    43981 cttctaatca aaggcataag tccatttctg tttttttcat gctggtaaga cttccaggga
    44041 gtggttatct ctggccctgc accagggtta atttggcaat tatgcatgct gaacattctt
    44101 ccagcatgtt tactgtcacg ggcgttaaga cctaaaactg tccccgtttc acctatgcgt
    44161 tctctcccac catttccgtt atgtgcaaca acttgattta tacctaacat ttgtgaacat
    44221 gctaatagtg cttcaagttt aggtctgtaa tctgtcgaaa cagaaaaata acgattaaag
    44281 gatcgacaag agatagcccg gctagtttta aactttttgc cattaattat agcatgatgc
    44341 tttttattaa taatttccgc taattcgacc ggatcgagag cattggtcat ttcatcaata
    44401 gagacagtta tcggtcctat aatgtaacgt tttcgctgat catcaaaagt tatcccatga
    44461 tgatttgcca ttattttgta tttggggtca tagaaacata caggataatc ttttatcccg
    44521 tcgtaggtat cctcatcaag tggtttcatg cgcgtgaagt tttgcatata gttaccattg
    44581 caattcgttt catgatttcc agctaataca tgaacatttt cattgatact cattatgcgt
    44641 tttaacaaat cgatgataaa ttgatcgcca cagatattac taaatcgatc gccggtttgg
    44701 tcgccaagaa acgtacatgg cgtgactgcg tctttattaa taaccaaata aggctgaaga
    44761 ctttctaaaa cattatctct tctgttcagt atactgatta ttgtattaac attagtgtca
    44821 agttgctcat taaagttctc ccttagcaat acgttgagaa cttcatcttc tatattaagc
    44881 aactggctaa ggaaattttc tccttcagcc atgattccca aatgtcctgt tgcaaaaagc
    44941 gcgtacaaaa ctgcaccgac tgagccatcg gtatcgccaa aataggtcgg cctggtactt
    45001 gtgggatcaa caggtgaaca attggagaaa taggttctgt cagcgaactg gggttttata
    45061 attgtttcga atttaatatc aaatacattg tattgcgata tttcccagtt ccagatgggt
    45121 ttgtacgact cagtaactgc aacatcatta gcattatcat tt
//
LOCUS       DAZSNM010000011        44867 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZSNM010000011 DAZSNM010000000
VERSION     DAZSNM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44867
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.011"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..307)
                     /locus_tag="ACHW42_000520"
     CDS             complement(<1..307)
                     /locus_tag="ACHW42_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFR8942481.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     "
     gene            482..2248
                     /gene="adeD"
                     /locus_tag="ACHW42_000521"
     CDS             482..2248
                     /gene="adeD"
                     /locus_tag="ACHW42_000521"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR8942482.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(2294..3685)
                     /gene="uhpT"
                     /locus_tag="ACHW42_000522"
     CDS             complement(2294..3685)
                     /gene="uhpT"
                     /locus_tag="ACHW42_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR8942483.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3823..5142)
                     /gene="uhpC"
                     /locus_tag="ACHW42_000523"
     CDS             complement(3823..5142)
                     /gene="uhpC"
                     /locus_tag="ACHW42_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR8942484.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(5152..6654)
                     /gene="uhpB"
                     /locus_tag="ACHW42_000524"
     CDS             complement(5152..6654)
                     /gene="uhpB"
                     /locus_tag="ACHW42_000524"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR8942485.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6654..7244)
                     /gene="uhpA"
                     /locus_tag="ACHW42_000525"
     CDS             complement(6654..7244)
                     /gene="uhpA"
                     /locus_tag="ACHW42_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR8942486.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7319..7609)
                     /gene="ilvN"
                     /locus_tag="ACHW42_000526"
     CDS             complement(7319..7609)
                     /gene="ilvN"
                     /locus_tag="ACHW42_000526"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR8942487.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7613..9301)
                     /gene="ilvB"
                     /locus_tag="ACHW42_000527"
     CDS             complement(7613..9301)
                     /gene="ilvB"
                     /locus_tag="ACHW42_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR8942488.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9407..9505)
                     /gene="ivbL"
                     /locus_tag="ACHW42_000528"
     CDS             complement(9407..9505)
                     /gene="ivbL"
                     /locus_tag="ACHW42_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR8942489.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            10070..10159
                     /gene="tisB"
                     /locus_tag="ACHW42_000529"
     CDS             10070..10159
                     /gene="tisB"
                     /locus_tag="ACHW42_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR8942490.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(10283..10357)
                     /gene="ysdE"
                     /locus_tag="ACHW42_000530"
     CDS             complement(10283..10357)
                     /gene="ysdE"
                     /locus_tag="ACHW42_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR8942491.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10439..11623
                     /gene="emrD"
                     /locus_tag="ACHW42_000531"
     CDS             10439..11623
                     /gene="emrD"
                     /locus_tag="ACHW42_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR8942492.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11631..12128)
                     /locus_tag="ACHW42_000532"
     CDS             complement(11631..12128)
                     /locus_tag="ACHW42_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR8942493.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(12125..12487)
                     /locus_tag="ACHW42_000533"
     CDS             complement(12125..12487)
                     /locus_tag="ACHW42_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR8942494.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12477..12824)
                     /locus_tag="ACHW42_000534"
     CDS             complement(12477..12824)
                     /locus_tag="ACHW42_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR8942495.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12933..13346
                     /gene="yidI"
                     /locus_tag="ACHW42_000535"
     CDS             12933..13346
                     /gene="yidI"
                     /locus_tag="ACHW42_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR8942496.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13393..14835)
                     /locus_tag="ACHW42_000536"
                     /pseudo
     CDS             complement(13393..14835)
                     /locus_tag="ACHW42_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(14870..16585)
                     /locus_tag="ACHW42_000537"
     CDS             complement(14870..16585)
                     /locus_tag="ACHW42_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFR8942497.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            16752..17645
                     /locus_tag="ACHW42_000538"
     CDS             16752..17645
                     /locus_tag="ACHW42_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR8942498.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17642..18456)
                     /locus_tag="ACHW42_000539"
                     /pseudo
     CDS             complement(17642..18456)
                     /locus_tag="ACHW42_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18456..20072)
                     /locus_tag="ACHW42_000540"
     CDS             complement(18456..20072)
                     /locus_tag="ACHW42_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR8942499.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            20367..21041
                     /locus_tag="ACHW42_000541"
     CDS             20367..21041
                     /locus_tag="ACHW42_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR8942500.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(21080..22741)
                     /locus_tag="ACHW42_000542"
     CDS             complement(21080..22741)
                     /locus_tag="ACHW42_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR8942501.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22937..23365)
                     /gene="ibpB"
                     /locus_tag="ACHW42_000543"
     CDS             complement(22937..23365)
                     /gene="ibpB"
                     /locus_tag="ACHW42_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR8942502.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(23477..23890)
                     /gene="ibpA"
                     /locus_tag="ACHW42_000544"
     CDS             complement(23477..23890)
                     /gene="ibpA"
                     /locus_tag="ACHW42_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR8942503.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23884..23956)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            24195..24527
                     /locus_tag="ACHW42_000545"
     CDS             24195..24527
                     /locus_tag="ACHW42_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR8942504.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24529..25761)
                     /locus_tag="ACHW42_000546"
     CDS             complement(24529..25761)
                     /locus_tag="ACHW42_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR8942505.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            25862..26962
                     /gene="cbrA"
                     /locus_tag="ACHW42_000547"
     CDS             25862..26962
                     /gene="cbrA"
                     /locus_tag="ACHW42_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR8942506.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(26923..28260)
                     /locus_tag="ACHW42_000548"
     CDS             complement(26923..28260)
                     /locus_tag="ACHW42_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8942507.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(28335..29483)
                     /gene="dgoD"
                     /locus_tag="ACHW42_000549"
                     /pseudo
     CDS             complement(28335..29483)
                     /gene="dgoD"
                     /locus_tag="ACHW42_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(29480..30097)
                     /gene="dgoA"
                     /locus_tag="ACHW42_000550"
     CDS             complement(29480..30097)
                     /gene="dgoA"
                     /locus_tag="ACHW42_000550"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR8942508.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(30081..30959)
                     /gene="dgoK"
                     /locus_tag="ACHW42_000551"
     CDS             complement(30081..30959)
                     /gene="dgoK"
                     /locus_tag="ACHW42_000551"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR8942509.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30956..31645)
                     /gene="dgoR"
                     /locus_tag="ACHW42_000552"
     CDS             complement(30956..31645)
                     /gene="dgoR"
                     /locus_tag="ACHW42_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR8942510.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31923..32579
                     /gene="yidX"
                     /locus_tag="ACHW42_000553"
     CDS             31923..32579
                     /gene="yidX"
                     /locus_tag="ACHW42_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFR8942511.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(32625..33437)
                     /gene="yidA"
                     /locus_tag="ACHW42_000554"
     CDS             complement(32625..33437)
                     /gene="yidA"
                     /locus_tag="ACHW42_000554"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR8942512.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33551..33949)
                     /locus_tag="ACHW42_000555"
     CDS             complement(33551..33949)
                     /locus_tag="ACHW42_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR8942513.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(34189..36603)
                     /gene="gyrB"
                     /locus_tag="ACHW42_000556"
     CDS             complement(34189..36603)
                     /gene="gyrB"
                     /locus_tag="ACHW42_000556"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR8942514.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36632..37705)
                     /gene="recF"
                     /locus_tag="ACHW42_000557"
     CDS             complement(36632..37705)
                     /gene="recF"
                     /locus_tag="ACHW42_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR8942515.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37705..38805)
                     /gene="dnaN"
                     /locus_tag="ACHW42_000558"
     CDS             complement(37705..38805)
                     /gene="dnaN"
                     /locus_tag="ACHW42_000558"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR8942516.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38810..40213)
                     /gene="dnaA"
                     /locus_tag="ACHW42_000559"
     CDS             complement(38810..40213)
                     /gene="dnaA"
                     /locus_tag="ACHW42_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR8942517.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            40510..40590
                     /locus_tag="ACHW42_000560"
     CDS             40510..40590
                     /locus_tag="ACHW42_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942518.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40820..40960
                     /gene="rpmH"
                     /locus_tag="ACHW42_000561"
     CDS             40820..40960
                     /gene="rpmH"
                     /locus_tag="ACHW42_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR8942519.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40977..41336
                     /gene="rnpA"
                     /locus_tag="ACHW42_000562"
     CDS             40977..41336
                     /gene="rnpA"
                     /locus_tag="ACHW42_000562"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR8942520.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            41300..41557
                     /gene="yidD"
                     /locus_tag="ACHW42_000563"
     CDS             41300..41557
                     /gene="yidD"
                     /locus_tag="ACHW42_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR8942521.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41560..43206
                     /gene="yidC"
                     /locus_tag="ACHW42_000564"
     CDS             41560..43206
                     /gene="yidC"
                     /locus_tag="ACHW42_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR8942522.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            43312..44676
                     /gene="mnmE"
                     /locus_tag="ACHW42_000565"
     CDS             43312..44676
                     /gene="mnmE"
                     /locus_tag="ACHW42_000565"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR8942523.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        10912 a        12141 c        11442 g        10372 t
ORIGIN      
        1 ccacgaacgc aaagaaggcg ttcagcccca ttgccggagc cagcgccacg ggtaagttag
       61 caaatatccc catcgcaata ctgccgatac cggcaatcaa acaggtggta acaaacacca
      121 ctttcgggtc catttgtgcc gcgccgagga tttgcgggtt cacaaaaaca atgtacacca
      181 tggttaaaaa agtggtcatc cccgcaatca attctgtgcg gacggtggtc ccatgctgag
      241 gtagtttaaa taatcgcgaa agcgtccctg attcattact cacgtaatcg gtattgtcat
      301 tattcatttt tttgtccatt ggaggagatt taatcccttg cttttgtaaa acaaatgaca
      361 acaacggttc agtgataatt atcacatttc aattgcacat taatagatat tctttaataa
      421 tctcgcgacg tttctttatg ataaataata atcaaattga taaaatcaaa atgagaaaaa
      481 tatgaataat tccattaacc ataaatttca tcacattagc cgggctgaat accaggaatt
      541 gttagccgtt tcccgtggcg acgctgttgc cgattatatt attgataatg tctctattct
      601 cgacctgatc aatgccggag aaatttccgg cccaattgtg attaaaggac gttacattgc
      661 cggtgttggc gcagaatacg ctgatactcc ggctttgcag cggattgatg ctcgcggcgc
      721 aacggcggtg ccagggttta ttgatgctca cctgcatatt gaatccagca tgatgacgcc
      781 ggtcactttt gaaaccgcta ccctgccgcg cggcctgacg accgttattt gcgaccctca
      841 tgaaatcgtc aacgtgatgg gagaagccgg attcgcctgg tttgcccgct gtgccgaaca
      901 ggcaaggcaa aaccagtact tacaggtcag ctcttgcgtg cccgctctgg aaggctgcga
      961 tgttaacggt gccagtttta cccttgaaca gatgctcgcc tggcgggacc atccgcaggt
     1021 taccggcctt gcagaaatga tggactaccc tggcgtaatt aacgggcaga atgcgctgct
     1081 cgataaactc gatgcatttc gctacctgac gctggacggt cactgtccgg gtttgggtgg
     1141 caaagaactt aacgcctata ttgctgcggg tattgaaaac tgccacgaaa gttatcagct
     1201 ggaagaagga cgccggaaat tacaactcgg catgtcgttg atgatccgcg aagggtccgc
     1261 tgccagcaat ctcaacgcac tggcaccgtt gatcaacgaa tttaacagcc cgcaatgcat
     1321 gctctgtacc gatgaccgta acccgtggga gatcgcccat gaagggcata tcgatgcctt
     1381 aattcgccgc ctgatcgaac aacacaatgt gccgctgcat gtggcatatc gcgtcgccag
     1441 ctggtcgacg gcgcgccact ttggtctgaa tcacctcggc ttactggcac ccggcaagca
     1501 ggccgatatc gtcctgttga gcgatgcgcg taaggtcacg gtgcagcagg tactggtgaa
     1561 aggcgaaccg attgatgcgc aaaccttaca ggcggaagag tcggcgagac tggcgctgtc
     1621 tgccccgcca tatggcaata ccattgcccg ccagccagtt tccgccagcg actttgccct
     1681 gcaattcacg cccggaaaac gctatcgggt cattgacgtc atccataacg aattgattac
     1741 gcactcccgc tccagcgtct acagcgaaaa tggttttgat cgcgatgatg tgtgctttat
     1801 tgccgtactt gagcgttacg ggcaacggct ggctccggcc tgtggtttgc tcggcggctt
     1861 tggcctgaat gaaggcgcgc tggctgcgac agtcagccat gacagccata atattgtggt
     1921 gatcggtcgc agtgccgaag agatggcgct ggcggtcaat caggtgattc aggatggcgg
     1981 cgggctgtgc gtggtacgta acggccaagt acaaagtcat ctgccgttac ccattgccgg
     2041 gctgatgagc accgacacgg cgcagtcgct ggcggaacaa attgacgcct tgaaagccgc
     2101 cgcccgtgaa tgcggtccgt tacccgatga gccgtttatt cagatggcgt ttctttctct
     2161 gccagtgatc cccgcgctaa aactaaccag tcaggggcta tttgatggcg agaagtttgc
     2221 cttcactacg ctggaagtca cggaataata aaaaaagccc ggcgtcatgc cgggcaaaag
     2281 tcaccagtta cgtttatgcc actgtcaact gctgaatttt tttctcgcgg cggattttgc
     2341 gttcttccat taccgccact atcgccatca ggcagataca accaatcgcg gcgatatcca
     2401 gcgcggcgaa ggtgcctgcc cagccggtaa ggccgaatac cggcgtccca tcggcaatca
     2461 ttcccagacc taacttggca aagctgtcac caatcaggta agcaaaggtg cctttaatac
     2521 catcggcagc gccaatcgct tttttaggta caaagccaac agcagccaca ccaatcaaca
     2581 attgcgggcc aaagaccagg aaacccaacg caaagagaga agccagatag atatattcgt
     2641 tactggcatg ttgatacaca ccgagcgtgg cgataatcag cgccagcgcg atgcaggcca
     2701 ccaggccacg gcgaccgttc gccaggtcag agagccagcc ccacagcagc gtaccgacca
     2761 gcgcaccagc ttcaaacagc gtaaagccct gaatcgccac cgctttagag agtttcagtt
     2821 cctggaacgc gtatacggtt gaccactggt cgataccaat acgtaccaca tagaggaaaa
     2881 tgttggcgaa gcacagcagc cagatcactt tgtttttcag cacatactca acaaagatct
     2941 gccacttggt catatcggta gattctgtct ctttgtcctc ttcgctgatc tcctcgccga
     3001 acagttcttc agctttgccg aggccataag attccgggga gtcgctgccg taacgcaggc
     3061 cgataaaacc gacaatcagc gcgataatcg acgggaagat aaacatgccg atgacatggc
     3121 catcgaacag gtaatttgcc ccgaacagcg ccacacctgc tgcgcctgca ccgccaaggt
     3181 tgtgagaaat attccagaaa ccgaggaatg tcccgcgttt acgacgcggc gtccatttgg
     3241 tgatggtgga gtaactgcac gaaccgccgg tactctggaa aaagccgctt aaggcgtaga
     3301 aggcaatcat caggaacagg ctaaccgagc cgctgcccat actggcactg aagcccagca
     3361 tacaaatggc agagaggata agcatgaacg gcaggaattg tttggtgttt ttgccgtcgg
     3421 cgtagtatga aaccaacgtt ttacccacgc cataagtgat ggagaaaccc aggccgatca
     3481 tccccagctg cgtcatgctc aacccgtagg tcgaaatcat atcgttctgc gcgatgttaa
     3541 agttcttgcg aatcaggtac atcgtcaggt agccgataaa gaccaccagg taggattgca
     3601 tgaacggttt gaaccacatt ttgcgccgca cttcgagcgg aaggtccagg gtcggcttgc
     3661 gaacttggtt taggaaagcc agcatgggtt actcctgaaa tgaatacctg cgtgaggcat
     3721 gcattgtaaa aatcagcagc aaatcaggcc tgagatagcg tccaagaaaa acctggaaaa
     3781 tttcttagtt ttgccggata tgaagtgaaa aggtgagatg catcacgctt cacgcggtgt
     3841 ctgggcgttc aaaaagggca gtaacagcag tgcggaaatc ccggcggcga tagagataac
     3901 cacaaaaaat ccgctccagt gccaggtatc gagcactttc gccagcggcc aaccagcaag
     3961 cgacgccccc agataagcaa acaagccgac aaaccccgtc gccgcccctg ccgcctcttt
     4021 gtgggaacac tctgccgccg ccataccaat taacatctgc gggccaaaga caaaaaaacc
     4081 aatggtgaag aagcaggttg cctgcatcac gtagctggca aatggcatca gccacagcga
     4141 gccgactgaa agcaaaattc cggcggcgaa aatcaaattc atcggcccac ggttgccgtt
     4201 aaacaatttg tccgagcccc aaccggctac cagcgcaccg ataaatccgc ccagttcaaa
     4261 catcgtcact gccgtattcg ccgtgaccag atcgacgccc agcgtctcgg acatatacaa
     4321 attgccccag tcgttgatcg ccgcccggac cacatagacc agcacatagc aaaacgaaag
     4381 cagccagata tacggattca gcaacacata tttggtgagg atctctttac gcgtcaaccc
     4441 tgccccttct tgttgttgag caatttccag cgcgtcgtgc cgccattcac cgaccgccgg
     4501 taaacctaac gcctgcgggc gatcgcgtag ccgccagcag agaaaaatcc ccacgactat
     4561 cgccatacaa ccagcaatca tcatcccggc acgccagccg taatgcagcg cgctcgctgc
     4621 catcacaatg ggaatgagtg cgccgccgac gttatgcgcc gtgttccata atgcccacca
     4681 accgccgcgc tcggtacgtg aataccaggc cgttaacaga cgcgcacaca ccggtgaacc
     4741 ccagccctgg aaaaaggcgt tcagcaccca gagcacggca aacgcccata gcgacgtcga
     4801 gaagccaaac agaatgttca tgatgcccgt ggcgataagc cctatcccca taaaataacg
     4861 ggcatttgag cgatcgctga caatgccgga gacaaacttc gacacgccgt aagtaatgta
     4921 aaacagggtc gctaacaggc cgatatcgct acggctgagc acgccgttag caaggatttc
     4981 tggtaccgcg gcgttaaaac ttttccgcgt gaagtaaaac agcgcgtaac ccagccagat
     5041 ggtcagcaga atatgccgac gccagtagcg ataacgggca tcaatttcat gtttatcagt
     5101 cattaatggc gcatcggcag gcgctttcag aaacggcaac atcatcgaac cttagacata
     5161 acgttgaggt agagaaacgc tgacacgcgt gccgtgcaga caggaaatgt gtaatgtgcc
     5221 gcccagcgcc gttacgcgct cgcgcattcc ggtgaggcca aaaccttgtt gcccggaacc
     5281 cggcggcaaa ccgctgccat cgtcttcaat caccagcatc aaccgttcat cctgctgcca
     5341 gccttgcagc gtaaccgcgc tggcatcggc atgtttcaca atgttgttca gcccttcctg
     5401 gcagacacga aacagcgtca cgcgctggtt ttcgcttaat gctgattcat cgatacgcca
     5461 ttcgagatgg ctgacaatgc cgcgcccttc cagctccatt tcccgcatca gtgagcggat
     5521 ggcctgctcc agggtgagat catccagctg gcgcggacgt aaccgtccta acaaacggcg
     5581 caccgcgtcg taaacgccca gcgacagttg ttcgatgagc tgcccgctct gcttcacact
     5641 ggcgttatct gccgccagcc gctgaacaat gcccgcctga gtacgaatag cagtgatggt
     5701 ctgaccgata tcgtcgtgca gctcacgcgc cacgtcgcgg cgcacgctct cttcggtttc
     5761 taacaaacgt tcagccagat gctgattgcg cgccagttcc ttttgtagcg actggttaag
     5821 ttcacgcaac cgctggatgc ctgcgcccag taacaaccct gtcagacttt gcaccagcaa
     5881 cgagagtaat aaatccacca gatgatcgcg ccaggtttga ctggcgatca gcgcgatggc
     5941 gttcatcaac gtcgcaatca gcgctccttg ccagccatag tgccaggcca gcgcgataat
     6001 cggcagcgcc agacagaatg gcgtaaagcg cgacagttcg tccggcaatc ccaactggag
     6061 ccagagactg ataacaaaca gcagcaagta ccagaccaga tgccgcccgc gccagttgat
     6121 gggctgagaa accagtgacg ggccgagcgg cagccaggtg ttattggcga gatagtgcca
     6181 gaacaccaga catatcgggg ccagcgtcag accgccagtt aaagtcagca acagcgcatt
     6241 ccacgactct ttgccgtgcc aaagccaggg cagcgactgc aacaacgccg ccgccgttaa
     6301 cgccgcaccc tgtaacagca aggtgcgcca gtcacgctga tggcgatagc gcgagatcag
     6361 cgctacgggc agtaacgtca gcaaactacc gatcatcaat aacggaaaat gggttaaacc
     6421 gaccgcctgc gttagccagt aaatcagcag ccactccgca cccagcaata cgggccagta
     6481 tccgcgcggg cattgcagca ttagccccag acgcagacca aacggaaata acagcaccgc
     6541 catatcaggg cgctcaacca gatgcaggct gatactccat aggcaaaacc atgcggcaga
     6601 gaagataaaa aagcaggcaa taacggtaat taagcgggag aacaacgtct tcatcaccag
     6661 ccatcaaaca tgcggcgcgc cagctctacg tcgttactga cgcccagttt ttccatcaga
     6721 ttggcgcgat ggacgtgtac cgttttcggt gacaagccca gttcggcggc aatctctttc
     6781 accgccattc cttgcgccag tttttccgcc acctggcgtt cacgtttggt cagcgggtcc
     6841 tgacgaccgg atgccagttt aatggcaata tccggcgtca gataacagcc gcccgtggca
     6901 accgtatgca ccgcagcaat gagttcatcc gggctacagc gtttggaaag aaagccgcgt
     6961 gcccccgcgt taagcgcctg ctcaaccagc gcaggactgt cgtgaacgga gagcataatc
     7021 gtcgccatac ctttcggcag ctggcttagc agctccagac cggagatatc gggcatggag
     7081 atatcgcaaa tacacacctg cacaccgcgc cccggcagcc ccgccagcgc ctcgcgcccc
     7141 gaaccaaact cggcaactac ctgcaaatca ggttccagcc ccagcagctg cgcaaagccg
     7201 gagcggacga tgaggtgatc gtctataagg gcaacggtga tcatggtctt gtcctggcgg
     7261 gtaaaaaaaa acgcgcttac cttaacgata agcgcgatgt tgttcaagcc ttgagcggtt
     7321 actgaaaaaa caccgcgatc ttgttaaaca tcgtcggatc ggactgatta cgctgcactt
     7381 tcacgacatc ttccagctta gcgatttggc ttatcatctg ctccagacgc tggtcgtcat
     7441 tgaccagtag ccagatatgg cttttgtcgc tgtcctgaat cggcagacaa agaatgcctt
     7501 caacgttaaa agcgcggcgg gcaaaaaggc cacaaacgtg ggtcattacg cccggatggt
     7561 tgcgaacggt gagctccaga attacgttgt tatgagttgt gttttgcatg gcttattccc
     7621 ccaccatttc agtattcgcc gcacctggcg gcaccatcgg gtaaactttt tcttcggcat
     7681 caatgcgcac atggatcagc gccgggccag ggcgattgat gatttcctgc aatgcagcct
     7741 gcggatcggc ttcgttattc aaatcacagg tttcgaggcc gaatccggcg gcaatctgca
     7801 taaagttgat tttgcccgga taggtggcgg caaaaacgcc ttgctcgtag aacagactct
     7861 gttgctgatg caccagcccc agcgcttcgt tgttcatcag aatgattttg acatccagct
     7921 gattttcact ggcggtcgcc atctcctgaa tattcatcat caggctgccg tcgccggaga
     7981 aacacaacac tttgcgatcc gggttcgcca gcgccgcgcc aatcgccgca ggcaggccaa
     8041 aacccatcgt gcccagccca ccggaggtca gccactggcg tgggcgattg agcggataag
     8101 cttgcgcggt ccacatctga tgctgaccca catcggtggt gataatcgca ttgtcatcga
     8161 cacaggcggc aacggcgttg atcaggccgt aatggcttaa tggatcgcac gctttcggga
     8221 ttggacacgg gaattcgcgc tgcaaatccg ctaccaactg gtgccactct gcacgcggtt
     8281 gcgcttccac ccgcgggatc aattgcgcca gcacgtcatc aacatccgcc tgaatcgcca
     8341 catgcggctg cttgatttta cccagctctg cgcgatcgat atcgacatga ataattttgg
     8401 cattcggaca gaactgctcg gttttgccaa tcgcccggtc atcaaaacgc gcaccgagca
     8461 caatcaacaa atccgcctcc tgcaaaatat agttggtact gcgcacgccg tgcatcccca
     8521 gcatacccag cgacaacgga tgcgcttttg gcagcatgcc cagcgccatt aaagtcatgg
     8581 tggtaggcag ttgcgctttc tccgccagtt cacgcacccg cgcaggcgca ttgatcacac
     8641 caccacccag ataaagcacc gggcgtttgg cagcgttaat cattgtagct gcgtcacgaa
     8701 tgctttcttc gctaaaggcg ggtgcaacgg ctttttctgc caccgcgggc tgagcttcaa
     8761 tctcaaaaac cgccgtttgt acatccttag gaatgtctat ccacaccggg ctagggcggc
     8821 ctgattgcgc aatgcggaag gcatcgctca tgacctgtgg gagctcttcg atatgtctga
     8881 ccagatagtt gtgtttggtg atggggatag agatgccgta ggtgtccact tcttggaagg
     8941 cgtcggtgcc gatcatcgag gcgggaacct gaccagtgat gcaaatcagc gggatggagt
     9001 ccagccgcgc atcagcaatg gcggtcacca ggttagtcgc acccggcccg ctacaggcca
     9061 tacagaccgc cggtttaccg tcggtgcgcg ccattccctg agcgataaac cccgcgccct
     9121 gttcatggcg agccagaata tggcggattt gcgtgctttg gcttaaggcg tcgtaaacag
     9181 gcaggataga accgcccgga atgcccgtca caatcttaat gccctgctgt tccaggaaat
     9241 gaacgataaa ttctgcgccg gtaaagcgct tacgcgtcga tgttgtgccc gaacttgcca
     9301 tgctccagtc cttttcttct gggccgactt tccgggaggt gcttaaacga aaaaccccgc
     9361 ccggtttgcg ccggcggggt tttggaatcg tgtgttgttc cagtccctac ggcgcattgc
     9421 cgacgaccac caccacacgc acgacgacca ctgcggcgga tggcgcagtt ggtagtagtt
     9481 ttgcgttgaa catggaagta gtcattgggg accttgttgg ttttgtgttt aacaatattt
     9541 atacaagcac agctttacag gggagacaat ggaaaatttt tcagcaaggg aaaattgagg
     9601 ggttgatcac gttttgtact gaattgcaga taacaaaaaa ccccgccgga gcgaggtttc
     9661 gtcagccgcc tgcggctggt aaccgcaaag cacactgtat tatgtcaaca ctgaaagtat
     9721 acgtgttccg cgcagaacgc gcaatttcgg cacgaatttt gacgtattta gtgcatagtt
     9781 gagtatcgtt cacagtttgc gttttgtcca aatattactg tttatttata cagtaagctt
     9841 ctataatatc actgtacgca atgtgttatg cgggggccgc atcgttaccc ggcgcactaa
     9901 gtcctggctg aaacgggtgg tgccgtcagc gccttaaccc cgcgtgagca cactgtgtta
     9961 tgtcaacaag cacaacgttt ctccttgaga taccgcgtgc acaacagctg gcaacaggca
    10021 gcggaaaggt acgtcagctg gcagtgctcc tgaaccacag gagacgcgta tgagcctggt
    10081 ggatatcgcc attcttatcc tcaaactcat tgttgcagca ctgcaactgc ttgatgctgt
    10141 tctgaaatac ctgaagtaat tcagattcaa gtcgcaccaa aggggagcgg gtaaccgctc
    10201 cccttttata tttagcgtgc gggttggagt cggatgcgat gctgacgcat cttatccgcc
    10261 ctaccatccc tcccggcaac atttattgcc gcttttgttt acatattctg ccgctaaaca
    10321 attccccatt cctggcgtat atctggctaa cattcatcaa tgtgatagat tcctctcccg
    10381 catttatggg aatgcgtagt gacttattct aattattttt ataaaagcat ccgtgataat
    10441 gaaaaggcaa agaaacgtca atttgttatt gatgttggta ttactcgtgg ccgtcggtca
    10501 gatggcgcaa accatttata ttccagctat tgccgatatg gcgcgcgatc tcaacgtccg
    10561 tgaaggggcg gtgcagagcg taatgggcgc ttatctgctg acttacggtg tctcacagct
    10621 gttttatggc ccgatatccg accgtgtcgg tcgccgacca gtgatcctcg tcggaatgtc
    10681 catttttatg ctggcaacgc tggtcgcggt cacgacctcc agtttgacgg tattgattgc
    10741 cgccagcgcg atgcagggga tgggcaccgg cgttggcggc gtaatggcgc gtactttgcc
    10801 gcgtgattta tatgaacgga cacagttgcg ccatgctaac agcctgttaa acatggggat
    10861 tctcgtcagt ccgttgctcg caccgctaat cggcggtctg ctggatacga tgtggaactg
    10921 gcgcgcctgt tatctctttt tgttggttct ttgtgctggt gtgaccttca gtatggcccg
    10981 ctggatgccg gaaacgcgtc cggtcgatgc accgcgcacg cgcctgctta ccagttataa
    11041 aacgcttttc ggtaacagcg gttttaactg ttatttgctg atgctgattg gcggtctggc
    11101 cgggattgct gcctttgaag cctgctccgg cgtgctgatg ggcgcggtgt tagggctgag
    11161 cagtatgacg gtcagtattt tgtttattct gccgattccg gcggcatttt ttggggcatg
    11221 gtttgccggg cgtcctaata aacgcttctc aacgttgatg tggcagtcgg ttatctgctg
    11281 cctgctggct ggcttgctga tgtggatccc cgactggttt ggcgtgatga atgtctggac
    11341 gctgctcgtt cccgccgcgc tgttcttttt cggtgccggg atgctgtttc cgctggcgac
    11401 cagcggcgcg atggagccgt tccccttcct ggcgggcacg gctggtgcgc tggtcggcgg
    11461 tctgcaaaac attggttccg gcgtgctggc gtcgctctct gcgatgttgc cgcaaaccgg
    11521 tcagggcagc ctggggttgt tgatgacctt aatgggattg ttgatcgtgc tgtgctggct
    11581 gccgctggcg acgcggatgt cgcatcaggg gcagcccgtt taagcgcacg tcaccgcagc
    11641 atcgtcatca gctccatggg agaacgatgc tgctttatca gatcacgcat cacccgcata
    11701 tgcggtgcgg agtaagaata aaacgcctga tagcccgcac aaagcacgct gtctggcgtc
    11761 tcctgccgag cgtgcgggca caggcgatgc cagtcgcacg cctggcagtc acggcgcggt
    11821 tgcgggtttt cgctgccaca ccatttttgt aaggtggcgt caaaaagctc aatcgagata
    11881 cgactgatat ctgcccgcac ccaacactca aacagggcaa ttaaaaattt cccccacagc
    11941 tctgctgtca ccgaagcggc cgtcagatcg ccccggtcat caaattcaag atagggaatg
    12001 agctgtacgg agcggatgcc ccgctgtttg aaattgtgat aaaaatcggc aggttgcaga
    12061 gcatccttgt actctacaac cagcttatgc ctgtcctctt cagcatcaat gacctgactt
    12121 cctgtcatgc gactctctca ataaatacga taagttgatg tatatgcgtt acagcaaaca
    12181 gtattgcgag agataacacc gcgagggaga tcaaaaattt gtcacggact acgtgaaatt
    12241 gggaaaaatc gctattcgtg acatccatta aattgcgatt acgggtgtag tgccagagga
    12301 tcagcgccac gatggcgagg atgccaatcg aaatccagaa taacatgccc gcctggtgcc
    12361 agttgtgttt gatagccaac gccatcaacg cgccgtagcc cagcatggta cgaaaccacg
    12421 ccagtgatgt gcgctccggc tgcagccctg ggtcggcaat gcgtctggct ttacggctat
    12481 ccggcataca acaccagtcc cataacgatc accgccacaa ccatcaatat taagctgatg
    12541 attaataagc tgttggtgta gggcaggtcc tctttcagac gcatcgcttt ttcattgcgt
    12601 aaccagcgca ggtagccata cattgctaac ccgccggaaa acagacacaa caacaacgcc
    12661 agcagttcac gaatgacggg cgtggcgaaa tctggcgcaa gttgatcaag cccaaccccc
    12721 gccgccagaa atcctagtgc ggtacggatc cacgccagaa aagtacgctc attagccagc
    12781 gagaagcggt aatcgggcgc ttctccaagg cgggaaattt tcattgttgc tcctttgcaa
    12841 tttcactaac gctctatctt tagcggtttt ttttaagaat ttcgtccgcc cgtttcctga
    12901 tatgcttttg ccatccgcta caaggaggtc agatgggtat catagcgcaa aataaaatca
    12961 gttcattggg catgctgttc ggtgccatcg ctttaatgat ggggattatc cacttttcat
    13021 tcggcccttt ttccgcacca ccgccaacgc ttgaaagcat cgtggcggac aaaacggctg
    13081 aaattaaaag aggtcttctc gccggaataa aaggcgaaaa aattaccact gtggagaaaa
    13141 aagaggacat ggatatcgat aaaatcctcg atcaaagcgg tatcgcactg gccattgctg
    13201 cgctactttg cgcatttatt ggcggtatgc gtaaggaaaa tcgctggggc attcgcggcg
    13261 cgctggtttt tggtatcggc atcgtgtgca gcattttgct gatattcctg attttttcct
    13321 tcctgaccgg cggttcgctg gtttaagcag cctgaataat gcccgccggg tgatcatcag
    13381 cgggcattat cgtcagaatt tctgtttttt ctcctccacc ttcactcctt gcgtcggtag
    13441 gccggtgtca tagtcgcgta ccactggcga atagccatct tgtgggcgtg ggcgaaacgc
    13501 ccccatccag cgcggtcgtg catctttacg ccacggacgc agactccatt ggtaactgcg
    13561 gaacggatcg cgaattttgt ccatgtaatc caataaggcg tcatgcattt tgctgcgaac
    13621 gtctgcaaaa cggatatcat cgatcaggtt atgcatttca tttgggtcat tacggcgatc
    13681 gtaaagttca tcactggtga agaggttgag caccagttta aagtcatccg tcacccagca
    13741 acgcaccgga ataaaaccgc caaagctgtc atgctcaatc tcgtagcggt taaattccac
    13801 catcacgccg cgtggctctt tcacggcaag gatattttcc cccggcagaa tctctggttt
    13861 ttcaatatct gccagcgcca tcattgtcgg cagtaaatca atatgactga ctggcgtatc
    13921 gacctgtcgc cgctcccctt gcggcgaacg gatgatcagc ggaatacggg tgatgtcgtc
    13981 atacatcgcc gctcctttac tgatcagctt atgtgcgccc atcatttcac cgtgatcgga
    14041 ggtataaatg acccacgtat tttcacgttg ctctggcgtt aaggcattga tgacacgtcc
    14101 gatttggtca tcaacaaagt cattgcaggc aaaatagagc ggatggtgat aaagcccgtc
    14161 atcaccgact ggcgatggca tcgcctgcgc ccataagcgg tgatgttccg gtttgttcgc
    14221 caggtcatcc tgtgatttct cgccaagttc gtagtaaaaa tcagcgtatt tctctaaata
    14281 ctccaccgga caggtgaacg ggtgatgcgg ctcatcatac gaaaccacca tcaggaaggg
    14341 ttcctcggcg cgcacgggct attgcagaaa atctaccgct cgattgctga tgcggtgcgc
    14401 ccaggtgaag gtttcgtcaa tatggttcgc ctgtaaatct tcgacgctgt ttaggccatt
    14461 gcgccacagg ctgatctctt tttccgtcag ttcgctaaga tagttcgccc catcaaacca
    14521 gtaatcagca tcccactccg gcggacactc gccagtgccg aaatagtcat gaccgtcgag
    14581 atgccatttg ccgatgtaac aggtgtgata gccggcatcc ttaaagtagc gccccatagt
    14641 ggagatgttt ttgcctggcg cgacgttgtt ggtccacggg ccggactggt tagcgtagat
    14701 accggtgaat agtccggccc gtgcaggcgt acaaaccggt gaacaagtgt aggcggaatt
    14761 aaagcgaata ccttccgccg ccagactatc aatattttgc gtattcagcg gtttaccgct
    14821 atagcaaccg accatattgg tggcctgggt atcggtcaat tggggcgttt cataggctat
    14881 ccttcgcgtc gggagtaaag gttacggctg ggtcgtgacg atgccaccag ctgtcaaaaa
    14941 tcaggtagac aatcactacg gcagcgatga aataactgat catcgctaac cagcgcgtac
    15001 cgtagccgcc gaattcagcc agcccgacat agacgccaat catcgcgaac aggataccaa
    15061 ttgacgcgat cttgacgttt ttccacggtt tcatgtccac cgcaaacgca tctttgaagg
    15121 tgaacggcgt ggcgcgcggt ttgataaaac cgatcaccag catcacgacc acgttgatgc
    15181 agaacgtaca ggccagcaca tagaggaaat ggaagtcgaa cttcaccaga tagttgatgg
    15241 tgatgtagct gattatgcca atccccatcg ccacttttgc cgccagcgcc gggatgcgcg
    15301 gaaagaaaaa gcccataatg atgatggtaa ccagcggcac gttgtaaatg ccgttgagct
    15361 gtttcatcca gctatacagc ccctgcggcg cgttcgcgat ccacggcgct accaaaaccg
    15421 aaacgatagc gataaagaaa ccaaatttgc gcccgacggt gaccagctgc tgcggctcgg
    15481 cattctggtt aatgatgcga cggtaaatac ccatactgaa taatgtactg gcgctattca
    15541 gaaagccgtt gaaggtactg atcaccgcac caaataacac cgcgccgaag aaaccctcca
    15601 gtggcactgg cagaacgtta ttgaccagcg tcgggtaggc catgtcggct ttcggtagat
    15661 cctgatacag atgaaacgca atcaaccctg gcagcaccag taccagcggg tcgagcattt
    15721 tcagcaccgc cgttaacagc gccccctttt gcccttccgc caggcttttt gacgccagcg
    15781 tgcgttgcac gatgccctga tttgtacacc agtaaaaggt gttcaccaga attaaaccgg
    15841 taaatgccgc gccaatcggc aagggatcgg tcgggccacc gactgagttt aatttctcgg
    15901 cgtgaacggt ggtaatttgc tcaatgccct gcataaagct gcccttgcct atcgcgatca
    15961 ggccaaatac cggcaccatc aacccgccga taaccagccc aataccgttg atggagtccg
    16021 ccactgccat tgcgcgcagt ccgccgatca ccgcatacaa aattcccgcc agaccaagca
    16081 aaattaccag tagccagata gccgcaccgt gagaaatctg tagcgattcc ccgacgtgaa
    16141 acaggctgtt gagcgccaac gcgccggagt agagaacaat cggcagaaag cagacgccgg
    16201 tggcaatgag gaagcagaag tcgatgataa tacgcgtcgt tttatcataa cgttcctcca
    16261 gaaaatcggg gatggtggca atgccgcgct tcagatagcg cggtaaaaag attagcgcga
    16321 ggaagatcag cgtcaccgct gaagtcactt cccagcccat caccgacatg ccgcttttgt
    16381 aggcctgccc ggaaagacca accagttgtt ccgtggaaag gttggttaac attaacgaag
    16441 cggcaataac cggcgctttt agtgaacgtc cggcaagaaa atagccttgt tgcgatccgg
    16501 tatctgtttt gcgaaccttc caccaggtga tcaccgccac cagcagcgta aaaccgacaa
    16561 aactcaagat ttgtaacgaa ttcatctcat agcccttatt tatgtttatg tcccggacgc
    16621 atgaatatag ggagaggtaa aatgcgtcgg gtcaggcagt cagattctta aaatacaaac
    16681 gtcgtatccc tgaacggatt gttttctgtt aagttcaggt tgtgagcata attcgccgga
    16741 gggatattaa aatgaatgga aaattgcaaa gttcagatgt aaaaaacgaa actccgtaca
    16801 atattccctt attgattaac gaaaatgtga tctccagcgg aatttctctg atctcgctct
    16861 ggcataccta cgccgacgag cattaccggg tgatctggcc gcgggacaag aagaaaccgc
    16921 ttatcgccaa ctcatgggtt gcggtgtata ccgtacaagg atgtgggaaa attcttttaa
    16981 agaatggcga acaaataact ctgcatagca actgtattat atttttaaag ccaatggata
    17041 ttcactctta tcactgtgaa ggtttagtct gggaacagta ctggatggaa tttaccccca
    17101 ccagtatgat ggatatttcc gttggtcagc aaagcgttat ttataatggc gaaatttata
    17161 atcaggaact caccgaagtt gctgagttaa taacttcacc agaagcaata aaaaataatc
    17221 tggcagtcgc ttttctgacg aaaattattt atcagtggat ttgtcttatg tccgcagatg
    17281 gtaaaaaaga tccacaacgg cggcaaattg aaaaattaat tgccacttta catgccagtc
    17341 tgcaacaacg ctggagcgta gctgatatgg ctgccacgat cccctgtagc gaagcctggt
    17401 tgcgtcgtct gtttttacgc tataccggca agacgccgaa agaatattac ctcgatgcgc
    17461 gtctggatct ggcgctatcg ctattaaagc aacaaggaaa ctcggttggc gaagtcgctg
    17521 atacgctcaa cttcttcgac tcctttcatt tcagcaaagc ctttaaacat aaatttggtt
    17581 atgcgccgtc agccgtgcta aagaatacgg accagcaccc aacggatgcc agtccacaca
    17641 attaacgtaa ctctggccag tacgctttgt tggcctccag caactcatcc agaatagctt
    17701 tggcgaccga ggcgctcggt accgttttcg acaacgtaat cgcctgccac aggtgctgat
    17761 atgaacgctg ttcccaggcg tccacgacca gtttttccac cgccacttgc tgactcatta
    17821 accctttctg aaactgcggg atatcgccga ccaccagtgg ttcaggccgt agtagcgcac
    17881 gcgcatctgt ttgcgatctt gcagcccaac aatttgcgcc atccggcttt caataccgat
    17941 tggcatgtca cagatgttga ggattttcgc attcgggcgc agacgacgcg tggcttctgc
    18001 gacaattgct gccgggttgg agtagttgag catctaggcg tttggtgaat atttttccat
    18061 ataatccacc agttccagga cgccgccaat ggaacgcatg ccgtacgcta ttccgccggg
    18121 tccgcaagtt tcctgaccaa caacaccgtg gcgcagtggg attttttcat ccagttcgcg
    18181 catcgggtac ttgccgacgc ggatgtgcgc catgacaaaa tcaacgtcgc tgaacgccac
    18241 ttcaggatcg gtggtgtaac taaacgcaat gtccggcgct ttttctttaa ggatgacttt
    18301 acaggcttcg gcaatcactt cctgccgcgc gccatcgtta tcataaaatt tcagtgcacg
    18361 aagcgggaaa cggtcctgat tcgccaggag catcaacacg atgcctggcg taaaggtgct
    18421 tccaccgcct gcgacaacca ctgagaattt ggtcattata ctgcctccgt aatggcaaca
    18481 ttttctgctg attgatgaga attaattaag ctatcgagct gttcacgcaa ttgggataca
    18541 tgcagaccaa tgatcacctg aatggcatcg ccactacgga agacgccgtg cgctcccagc
    18601 tttttaaaga cttcgtcatc cagcgtttgt gacatgtcat gcagtgcaat acgtagacgc
    18661 gtcgcgcaat tgttaatgct ggagatattg ccgaccccgc ccagggcttg caggataccg
    18721 gcagcctgat ccagctcttt ttttggctct gcagcggtgg tttggcctcg cgaggctttg
    18781 tattcggctt ttgagtagag tttcacttcc gcatcttcac gtcccggcgt gcacatgttg
    18841 aactgcagaa tcagggtgcg gaaaaccacg aagtacagca gggtaaagca caacccaatg
    18901 gcgatttggg tcagcatcat atccgcgtgg ttgctgaaca tcgggatcca gttttgcggt
    18961 aaaacctggt caatcagacc tccgcccatg ttgcccacca caccaaagag atacattacg
    19021 gtcgacattg aggccgccag cacggcgtgt accgcaaaca gcaacggtga aatgaacagg
    19081 aaggtaaatt ccagcggttc ggtaattccc accagcatgg cggttaaggt tgcgggaatc
    19141 agcaagcccg ccacttttac ccgattttcc ggtgcggcag tgaagtacat cgctaaagaa
    19201 atgcccacgg caccaaagat ttttgagtta ccgtgcaggg caaaaccgcc ttccgggaac
    19261 aacgatttca gtggctcggc gctcagactg aactcttgca gatgctgcgc ccagtacatc
    19321 tgaatgccgc cttcaacagc tgccggacca aagataaacg gtccgtagat gaagtggtgt
    19381 aaaccggttg ggatcagaat acgttcgagg aaggtgtaaa cccacacccc aagcgcaccc
    19441 gccgaacgca ggaacgcttg cagagattca atccccattt gtacttttgg ccagccgagc
    19501 aatgtcagcc aggcacaggg gatcatcacc aggaaggcga taatcaccac ataagacgtc
    19561 ccctggaaaa tgccgagaaa aaccggcagt tttttatcga acagacggtt atgcagcgcc
    19621 gtcacaatgc cggaaatgat aattgcgccg ataatgctgg tatcgagggt tttaatcccg
    19681 gccatcattg tcagaccgct acctgccacc gcgtcctgag tgaaatcgac gccgaagtag
    19741 cttccccagg tcattcccat cgcattgatg aaatagttcc aggtcaggaa actcaccatc
    19801 accgccagac aagcacgtcc ctgcgcttgc ttagcaaggc caatgggtaa accgacagca
    19861 aaaatcagcg gcatattacg gaataccgtc caaccgccct cttcaataat gtgtacgatt
    19921 tgcgcgaata aactgttcgg atcggtcagt gattccccga caaacatcgg gttttgcagc
    19981 aagatggcaa gacccaccac aatcccggcg aagggaaaca gcagcactgg cgtgaacatc
    20041 gcgccgccaa agcgttgaat ttgactgagc atttccacat cctcttttct caattctgta
    20101 agtaggaagc ctgattggtt atgcggcaga cctggagcga ggataggaag atgcgatcgg
    20161 cagaacatga cgacccaatg ggattcgtga tcgctttcat gcttttcgtt gggcggtaga
    20221 gatctacgtt ttcgcgaaaa agtagacatc tgtgagcaca ggtacacagc atcgaacaca
    20281 tctttaaaaa aaagattttt ttcaatcgat taagcagaac ttgtgggcgc attacccgtg
    20341 cttgcaggca aaaaagagag ctagagatga tctacaaaag cattgcggag cggttaagaa
    20401 ttcgacttaa ttccgcggat ttcacgctaa acagccttct tcccggtgaa aaaaagctgg
    20461 cggaagagtt tgcggtatcg cggatgacta tccgtaaagc cattgacctg ctggtagcgt
    20521 gggggctggt ggtccgccgc cacggcagcg gcacttacct ggtgcgcaaa gatgtgctgc
    20581 atcaaaccgc cagcctgacc ggactggtgg aggtgttaaa acggcaggga aaaacggtca
    20641 ccagccaggt gctgattttt gaaatcatgc ctgcgcctcc ggccattgcc agccagttac
    20701 ggattcaaat caacgagcag atctacttct cccgtcgcgt tcgttttgtg gaagggaaac
    20761 cgctaatgct ggaagacagc tatatgccgg taaaactgtt ccgtaacctt tcgctgcaac
    20821 atctggaagg gtcgaagttt gaatatattg aacaagagtg cgggattttg attggcggta
    20881 attatgaaag cctgatgccg gtgctcgccg atagactgct ggcgcggcaa atgaaggtag
    20941 cggaacacac gccactgctg cggatcacct cgttgtcata tagcgagagc ggggagtttt
    21001 tgaattattc agtgatgttc agaaatgcca gcgaatacta ggtggagtac catttacggc
    21061 gactccaccc ggaaaaaagt taaccgatac tccagaagag caccgccagt aattgggggg
    21121 tgataatgcg caggaacatc actaacggat agacagtggc gtaagagagc gccgccgcac
    21181 cactggttgg atgaagatta ttagcaaacg ccagcgccgg aggatcggtc atggaacctg
    21241 ccagcatccc gcacatggtc aggtaattca ttttggctaa catccgcgcc agaatgccaa
    21301 cagtaatcag cggaacggcg gtgatcaggg caccataacc aatccagctt agcccttcgc
    21361 cattgaccag ggtattcaca aaatccccac cagatttcag accaacgacc gagaggaaca
    21421 gcacgatccc cagctcccgc agcgcgaggt tggcacttgg cggcataaac cagtacagct
    21481 tgccgatact gccgatacgc ccgaggatca acgccataat cagcggaccg cccgccagcc
    21541 ccagtttcaa cgcggccggg aatcctggca caaagacggg aatagaacct aacaatacgc
    21601 caagcccgat gccaataaac actggcagca tctgaacctg ttgcagtttt tgctgcgcat
    21661 tccccagcac attggcaacg gcatcaattg cggacggacg ccccaccagg ttgaggatat
    21721 cgccgaactg caggctgata tcgccactgg cgaccagttc gaccccggca cggttcaggc
    21781 gcgagataac aacgtcatag cgttctttaa agtgcaggtc gcgaatacgt tttccgagca
    21841 cgttttcatt ggtgaccacc acacgctcga cgcgcaaatc agtgcctttc gtagacagcg
    21901 aggtatcgac ctcctgacca atcaccagtt gcgcattatg taaatccgct ggctgcccca
    21961 ccagatgcag caaatcgccc agttggataa tggtatctgg cgaaggtact tttagggttt
    22021 cttctcgttt cagacgcgag cagataattt tgtcgccgtt gagaatcggt acatctttaa
    22081 tggctaaatc atgcaggtta gggttctcaa cgcgaatatt gatagtcttg atcagcgcgc
    22141 cgccattggt gcgtgaagac tcgtgctgct gagcttctgt ctcgacattg acgcggaaaa
    22201 taacccgcaa catccacatg gtgaacaaaa tcccgcaaat gccgaatgga tacgccatcg
    22261 cgtaactcat ccccatctga tcgaccattt ccattggtgt acccaggtcg cgcaagatct
    22321 gctgccctgc ccccagcgct ggcgtattgg ttaccgcacc ggagaaaatc cccagcacta
    22381 ccggcagtgg aatatcaaac agtttatgca ggatggcggt aaccagacca ccgatgatga
    22441 cgatcagaac agcaaacagg ttgaggcgta atccggagac gcgcaatgag gcaaagaagc
    22501 ccggccctac ctgaatcccg atggtataaa cgaacaggat caggccaaat tcctgaataa
    22561 catgcagcat atcgctactt aatgtcatcc ccgcctgaga aacaaaatgg ccgacgatga
    22621 tcccaccaaa cagcacgccg ccaataccta atcctatgcc gcgaaatttg acgttgccga
    22681 taaacaaacc gacgactgcc accaaagcca gaatactgac cgttaatgct atatcactca
    22741 tcgtccattt ctcttgagat aacattttaa ttgctacgga ttatggctga atcggtgaag
    22801 cagatttgcc ttatagcgca cattatcacg ggaaagaacg tgccgaaata tcttaaacag
    22861 tcgaaattta acgcattgtt gaagaagaaa gacaaaaaaa gccccaccag aatggcgggg
    22921 caaagagaat agctagttag ctatttaacg cgggacgttc gctgatagcg atacgctgcg
    22981 ctgcgatggg ttcaggctca ttacgaatta aatcaatatg cagtaaaccg tttacgaagg
    23041 ttgcgccaga gacttccata ttttcagcca gcgtaaaact caggctaaat ggctgattca
    23101 taagcccttg atgcagccat tttttctctt cttttggctg ctccggcgtg ccttttacgc
    23161 tcaggcgcgt gccttccagt tgaatctcta aatcttcctg acggaaacct gccagcgcaa
    23221 gggtaatgcg gtagtggtta tcgtcgcttt tctcaatgtt gtacggcggg aagctctggc
    23281 tttcaccggc gttttgcagt gcgttggcca gtttgtcaaa accgatccat tgacgcatca
    23341 gtggggataa atcgaagtta cgcatagtca tttctccttc taagaagcga gtaagtacct
    23401 gcaaatccga agattcgcat atgctccctg acggcgagca tggagatgtc aggccgcgcc
    23461 aggcggcctt agggaattag ttgatttcga tacggcgcgg ttttttcgct tccggaatca
    23521 cgcgttcgag atcgatatac agcaaaccat ttaccaggtt agccccacga acatgaatgt
    23581 tctcagctaa ctggaatttg cgttcaaagt tgcgttcagc gatgccctgg tacagatagg
    23641 tgcgctcttt ttgttcgtcg gcgtgagcac ctttcaccac cagcagatta tcctgggcgg
    23701 taatttccag ttcgctctca gcaaaaccag ccacagcgat agcaatgcgg taatggtttt
    23761 cgtctaccag ttcaacgtta tacggagggt agccgccatt actctggctc tggttgtttt
    23821 ctaagtggtt aaacaaacgg tcaaatccaa tagcagaacg gtaaagcggg gataaatcaa
    23881 agttacgcat aatcaatagc tcctgaaatc agcgagaatg taagaccttc cacaatggac
    23941 aggtcaggta gccagaacac ccatcaggcg cgtcctcatc gactacgatg taaaatgggt
    24001 ctggaaatga acttttcaag ccttatcagc ggactttttt ttgcagttta tggtctattg
    24061 catagactga gggggcagca cgaaacgtta aagtgcgata gccgccacag tgcgacgtaa
    24121 tgatgatgtt attttcagca agggatcgct ataactcatc aggccaacac ccacgatcaa
    24181 cagatgaatt tattatgata agaaatgtgt tgttagcgtt catgatatgc agcggaatga
    24241 cattactcgg aggatgctcc agcgtgatgt cccataccgg ggggaaagaa ggaacgtatc
    24301 ccggcacccg cgccagcgcc accatgattg gcgacgatga aaccaactgg ggcactaagt
    24361 cgctggcgat tctcgatatg ccgtttaccg ctgtaatgga cacgctttta ctgccgtggg
    24421 atgtgttccg caaagacagc tcggtgcgct cgcgcgttga aaaaagcgaa gccaacgcgc
    24481 aagccaccaa tgcggttatt ccaccggcca gaatgcctga taactgattt agcgatcagt
    24541 ttcggtgatc cacagctggc caccttccat ccacgccagc cattgaccat ccggcgagaa
    24601 gaccacggcg tccgcagaag gtggattggc gtggttttcg gttaaatact caacctcgcc
    24661 gctttgcgca tgggcacaaa caattcgatt atccagcaca aagcccaacc attctcctga
    24721 cggatgccag ttaaatgcag actgaatatc cgttttgtta tgggttaact tgcgcggttc
    24781 gccgccctgt ggcgagataa gccacagttg cacaatgccg ttatcatcac gcattaaaaa
    24841 cgcgatttgc gtaccctgcg gattacagcg cacccagtgg cgcgggacgt tgactaaccc
    24901 cggataagcc cgatgatggg taaaggttaa acgtcgctgc acgacgccac gcggtggcgc
    24961 gggtagggtt gtttccgttc cacttaacgg cgcatcacct gccgatttcc agctagcttc
    25021 atcttgcggt aactcaacga taaatagctc cggcactttc tcgccctttg gcgaaagtgt
    25081 gtcgccaata aacgccagcg cgtgatttcc tacccatcct tcttcataag cacgattgat
    25141 ttcatcactg ccaggctgcg gcgtgggcgt ggttttactc accagcacac accagtggct
    25201 accgctgtat tcacgcggat gttgcttttg tacgttgacc gggccaaacg gtgcagcaac
    25261 cccgacgttt cgcaaatcca gcgccggatc gagttgatgc attacatggt cgttataggt
    25321 aaagctcccc ctttcaccgt tcgggctaaa gacatgcaca tggctgccac cgcgcagcgc
    25381 tcctggcgta tacggcgcgg taatatccat tgcatcgaga ttgctcacct tgccgccttc
    25441 agcaatgact ccgcgtcgat gatggaagtc gtaatgccat gtttcatcag gattttcagg
    25501 gccgtgaata aagacatatt tctctgactt cggatgaacg gtcaccacgc cgacgtgtgc
    25561 gccctgtgac gcgcgataga taacctcgac ctcgcctgta tggatattca cacgctcaat
    25621 ggtctcgccg gtaaacgacg ccccagaagg acgcacgtca aataccagcc actggctgtc
    25681 gggcgtccag gtattggtat ttgtattggt attggtattg gtgagcaggt gattacgggg
    25741 agcaaaggtt atctgtttca tggcgcggtc ctgatacaaa acggttcccg ccatcatact
    25801 tcacaaggac ttcactttca gacaatagtc ttttgccatc gtgatgaatg tgagtgaaaa
    25861 gatggaacat ttcgacgtgg cgattattgg cctcggcccg gcagggtcgg cgttggcacg
    25921 aaagttagcc ggcaaaatgc aggtgatcgc gctggataaa aagcaccagt gcggtactga
    25981 aggtttcagc aaaccttgcg gcggtctgct ggcaccggac gcgcagcgat cttttattcg
    26041 cgatggactg acgcttcctg tcgatgtgat cgccaatcca cagattttca gcgtcaaaac
    26101 tgtcgacgtc gccgcatcgc tcacgcgtaa ctaccagcga agctatatca atattaatcg
    26161 ccatgctttc gacttgtgga tgaaatcgct gatccccgcc agcgttgagg tttatcacga
    26221 tagcctgtgc cgaaaaatct ggcgtgagga tgataaatgg catgtcattt ttcgtgcaga
    26281 cggctgggag caacatatta ccgcccgcta tctggtcggt gccgatggcg caaactcgat
    26341 ggtgcggcga catctctacc cggatcatca aatccgtaaa tatgtcgcta tccagcagtg
    26401 gttcgcagag aaacatccgg tgccgttcta ctcctgcatc tttgataatg cgataactga
    26461 ctgttactca tggagtatca gcaaagatgg ttattttatc tttggcggtg cctatccaat
    26521 gaaagacggt cagacgcgtt tcacgacgct gaaagagaaa atgagcgcct ttcagttcca
    26581 gtttggcaaa gcggtgaaaa gtgagaaatg cacggtgttg tttccctcac gctggcagga
    26641 ttttgtctgt ggtaaggaca acgcctttct gattggtgaa gcggcgggat ttatcagcgc
    26701 cagctcgctg gaggggatta gctatgcgct ggatagcgca gagattctgc gtgcggtgtt
    26761 cctgaaacag ccggagaaga gcaacgccgc ttactggcgc gccacacaca aactgcgttt
    26821 aaaactcttc ggcaagatag taaaaagccg atgcctgacc gcaccggctt taagaaagtg
    26881 gattatgcgc agtggtgtgg cgcatattcc acagttgaaa gattatccaa cgcgcttcac
    26941 atcacccacc agcaggatgt aagagagcgc gccaatcaac gcgacggcgg agatataaac
    27001 cagtgcaggc gcgaaaccgt aaccctgcgc caggtacccc accaccagcg gaacggtgat
    27061 gccgcccaga ccaccggcga agttaaacac gccgccggtt aaaccaatca ggcgcatcgg
    27121 tgccagagaa gagaccagcg accaggtaat cgaagcaaaa ccgttgccga agaatgccag
    27181 cgccatcagg cacataatca tcatcggatc gttggtgtag ttagcgccca taatgcaggt
    27241 ggagatcagc aagccgcaga taatcggcgt tttacgcgca aagcccagtg aaaagccctt
    27301 acgtaccagc agatccgcga cccagccaga gagcaggacg ccgacaaacg ccgcgaggaa
    27361 tggcaccgtg gtcataaagc ctgctttcag cgccgtgatt cctttttcct gggttaaata
    27421 gttcgggaac caggttaaga aaaaccacag tgcagaagcc accgcaaatt gcccaagata
    27481 aacgccgatc agtttacgat ggaacaccag tttccagtct ttggctgtta acggctgacg
    27541 cgcctctttc ttcaccggcg catcaccatc caccagaccg ccgccatcac gaatgtaatc
    27601 cagttcagct ttgctgatac ctttggtcag gcgcggcggc tgataaacct taaaccaaat
    27661 cagcgaccag ataatgccga taccaccagt gacaatgaac acccagtgcc agctcaacat
    27721 ctcctgaatc cagatcagca gcggagtcaa aaacgccaga ccgacaaact gaccagacgt
    27781 ataaaaacca acggcggaag cgcgttcatg ttccgggaac cagctggtca ccatccggtt
    27841 attggtcggg aaagcgggcg cttcgaaaat accggttatc gcacgcagac caattaatga
    27901 cattaatccc gtggcaaagc cttggaataa agtcgccact gaccaaccaa atatcgcaat
    27961 aaaataagtc acgcgagaac ctacgcgatc taaaaaccaa ccgccgggga tctggcatag
    28021 cgtataaagc caggcgaagg ccgaaaatac atagcccatt tccgctttcg taatgccgaa
    28081 ctcttcctga atatgggcgg aagccacggc caggttagcg cggtcaacat aacaaatgac
    28141 caccgtaata aagatcatca ccagcgtcag ataacgccga cgccccggct ttgctgcatt
    28201 aacgggaata tccatagcga gctttctcca gattttgggc atagcgaagc cgctcaccat
    28261 gccctgtaat ttacagaggg ttatattttt gtattgctgt tttagtgccc gatgaggggc
    28321 ttacgtggca ggaattacca ctctgctacg ctgttatctt catgacgcca gagcggatta
    28381 cgccagtccg gggcattttt actgaactca ataactttag cttcgtcgat ttccacacct
    28441 aagcccggtt tcgttaacgg tttaaagaag ccgccaacca tgctgaagtc ttctttgttt
    28501 ttcacaaagt cgagtaactc cgcgcctttg ttgtaatgaa tgcccatact ttgttactga
    28561 agtaccgcgt tataggaaac gaagtcgata tgcaggcaag ccgccagtgc aatcggtccg
    28621 aacggacagt gcggcgcaag ggtcacatca taggcttctg ccattccagc aattttgtag
    28681 cattcggtaa taccgcctgc atgggagaga tccggttgca gaatcgaaat accgcctgcc
    28741 tccagcacgc gtttaaaatc gaaacgtgag aacatgcgct cacccgccgc cagtggaata
    28801 tgcgtttgtg ccgccaattt cgggtagtat tcggcttgtt ccgccagcac cggctcttca
    28861 ataaacagcg ggcgatacgg ctccagttct ttaatcagca ctttcgccat tggcgcgctg
    28921 acgcgaccgt ggaaatcaag accaaactca atctgattgc caaaagcttc acgaatttgt
    28981 gccacggtgt tgactgccgc atctaccgcg cgggagttat caattagccc cagttcttca
    29041 caaccgttca gtttgaaggt atcgaagccg atttcgcgca gggttttaat gccgtcgata
    29101 acatccgccg gacgatcgcc gccgacccaa ctgtaggctt taattttgtc gcgaaccagg
    29161 ccgcccatca gttgccagac tggcgcattc agcactttgc ctttgatatc ccataacgcc
    29221 tggtcgatcc cggcgatggc gctcatcagg attggaccgc cacgataaaa tccggcgcga
    29281 tacatcactt gccataagtc gttgatgcgc gaaggatcct gaccaatcaa atagtcaccc
    29341 agctcgtgaa ctgccgcttc caccgtacgg gcgcggcctt caatcacggg ctcgccccaa
    29401 ccgaccacgc cttcatcggt ttcaattttc aggaacatcc agcggggagg taaacgatac
    29461 gtggtaattt tggtgatttt cattgcactg cctctcgata cgccttaaca aatgctgctg
    29521 cctgctgcgc ggtgcgttct acggactgcc cggcgcgata aagatcgctg cctaagcccg
    29581 ccccaacaca acctgcgtct atccactgcg ccaggttttc tggcgtcacg ccgccaacgg
    29641 caaagactgc gatgtccggt ggtaataccg cttttaacgc tttgatgtat tgcggaccaa
    29701 aagccgatga cggaaatatt ttcagcgcct gcgcgcccgc ttcgagcgcg gtaaaggctt
    29761 cggtcgccgt cgcgcagccg gggcaaacgg tcatgccgta gcccaccgcg cggcggatca
    29821 cttcactatg gatattgggc gtaacgatga gctgacagcc catcctggcg agcgcatcga
    29881 cctgttcagg tttcagtacc gtacctgcgc caatcaacgc cttgtcgcca tacgcatcaa
    29941 cgatggcggg aatgctctgc tcccattgtg gggaattcag cgggatttca accgcgtcga
    30001 acccggcgtc aatcaccgcg ccaacatgcg ccagcgcctc gtcgggcgta atgccgcgca
    30061 aaatggcgat cagcgggagt ttagtttgcc actgcatgag cgatgctcct tataccagcc
    30121 tgaaatgccg tgtcgcccgc caccgccgcc acgtcgcaac ccatcgcctg aaaggcttgc
    30181 tggtagcgcg cggtcagcga tgttccggcg acaagggtga tggcgtgttg atgggtcaca
    30241 tagtcgcgca tactggcaac ctctgcgcca atcaacaaac cagagagaaa ttcgctgacc
    30301 tgttcgcggg gaagtgttcc cagcacatgc gaggcgcgaa cttcaaaaag ctgcggcaat
    30361 atggcgggcg cattaaggcc gcgctcaagg ccagccgcga aggcatcggc agagttttcc
    30421 tgcggcggca aacctgcgcc aatcaatgag tgatttaaca gtaaatgatg taattcaccg
    30481 gtcatcacgg tgcgaaaatc gttgatttgc tggctatcgg cctgcaccca tttgcaatgc
    30541 gtaccgggca taacataaag agaggaagga gccagagctc gcgcgccgat caattgtgtt
    30601 tcttcgccgc gcatcacatt gtggttatcg tcatgagaga cgcataatcc gggaataatc
    30661 cagatattgt cgccaacaga cgttaattgt tcgccaatag acgaaaaacg ggcaggaaca
    30721 gataaatacg gagcaacttt ccagccgaca ttgctgccga ccattcctgc catcaccact
    30781 ggcgtatttt cttcacgcca gtcggtcgtg acttctgcta acactgcagc cggagatttt
    30841 ccgttcaggc gcgtgacgcc tgcttctgat tgcctgctct ccaggcagtg gtcgccctga
    30901 taaagccagg cgcgcagatt ggtcgatccc cagtcaattg cgatgtagcg agctgtcatg
    30961 tgatttcctt taaccttcgt gtcgagctgg caatcatggt aagcgccgcc tgctctgccg
    31021 catcgccgtc ctgatgccgt atcgcatcga acagcgcctt atgttcctgg agcgtttgcg
    31081 gcatgttggc ctcatcgccc atccaggttc gttcaaaaac cgcccgctgc agcgaactga
    31141 tcgcaatgct aagttgctgt aacaccgggt tatgcaccga ctgcagcacc gcctcgtggt
    31201 agcgaatatc cgcttcgtta aacgcttcgc ggtcctgatt gttggcaatc atctcgttca
    31261 gcgccgattc aatctgcgcc agatcgctgg aggtcgcgcg ctctgctgcc caacgggcaa
    31321 tcgccggttc caccagattt cgcacttcgc tcatggcact gataagccgt gggtcgtagt
    31381 cgttttccag cacccattgc agtacgtcag tgtcgaggta attccactgg ttacgcggtg
    31441 ccacaaacgc cccgcgataa cgtttcattt caatcagccg cttcgccatc agcgaacgga
    31501 acacctcacg gatgatgttg cgcgaggttg caaactcttc acagagctcc gcctcagccg
    31561 caagtggcga gccaggtacg tatttgccgt gaacgatctg tttacccagc gtaatgacaa
    31621 tgcgatcggt tttattgaga gtcatggaga gtccttgtgc tctgtatgtt cttctctact
    31681 ttaccccgat cgatgcataa cgcggcaact ttgtagtacc agcgtgatga cgttcgcgtt
    31741 tgccgcgcgc gtaatgtagt acaaacttat attgttgcgc tacaatttag atcaaaaaaa
    31801 gaacaacaca taaaaaaatg acatgcgtcg ggcagaaatc tgaaaaggga tatcaggcgc
    31861 taaacaggag ggaaagaaga gtatgctttc aacggcttag ctactcgttt aaaggattaa
    31921 tcatgaagtt gaattttaag ggatttttta aggctgccgg tttattccca ctggcgctga
    31981 tgctttcagg ctgtatctcg tatgctctgg tttcccatac cgcaaagggt agttcaggaa
    32041 agtatcaatc gcagtcagac accatcactg ggctatcgca ggcaaaagat agtaatggaa
    32101 caaaaggcta tgtttttgta ggggaatcgc tggattacct tatcactgat ggtgccgatg
    32161 acatcgttaa gatgctcaat gatccagcac ttaaccggca caatattcag gttgccgatg
    32221 acgcaagatt tgttttaaat gcggggaaaa agaaatttac cggcacaata tcgctttact
    32281 actactggaa taacgaagaa gaaaaggcac tggcaacgca ttatggtttt gcctgtggtg
    32341 ttcaacactg taccaggtca ctggaaaacc taaaaggcac aatccatgag aaaaataaaa
    32401 acatggatta ctcaaaggtg atggcgttct accatccatt taaagtgcga ttttatgaat
    32461 actattcacc cagaggcatt ccggatggtg tttccgcagc attactgcca gtgactgtta
    32521 cactggacat cattactgca ccgctgcaat ttctggttgt atatgcagta aaccaataat
    32581 cagtaagcgg gcaaacgcgt ttatgctgtt tgcccgccca cagattaatt cagcacatac
    32641 ttctcaatag caaacgccac gccatcttca aggttggatt ttgtgacaaa gttcgccact
    32701 tctttcaccg aaggaatagc gttatccatc gccacaccga cgcctgcata ttcaatcatt
    32761 gcgatgtcgt tttcctgatc gccaatcgcc atgatttctt ccggtttaat acctaacacg
    32821 tcggccagtg atttcacacc cgtacctttg ttaacgcgtt tatcgaggat ttcgaggaag
    32881 tacggcgcac ttttcagcac ggtatatttc tctttcactt cctgcggaat acgcgcgata
    32941 gcctggtcga ggatggcggg ttcatcaatc atcatcactt tcaggaactg ggtattgggg
    33001 tccattttct ccgcttcgca gaacaccagc ggaattgtgg caacgaagga ttcatgcacc
    33061 gtgtagtagc tgatatcgcg gttagcggtg tacagcgtgg tgcggtccag ggcgtggaaa
    33121 tgagaaccga cttcgcgaga gagtttttcc aggaaacgat agtcgtcata gctgagagcg
    33181 gtttgcgcca cggtgctacc atcagcggcc ttctgtacca gcgcgccgtt ataagtaatg
    33241 cagtagtcgc ccggctgttc catatgcagc tctttcaggt agttgtgcac acctgcatac
    33301 ggacgacccg tcgttagcac gacattcacg ccacgggcgc gagctgcggc aatcgcattt
    33361 ttaacggcgg gtgaaatggt gtgatcgggc agcagaaggg tgccatccat atcgatagca
    33421 atgagtttga tagccatgag ttcccaaggt agattagttc ctgacccatg ctaacgcgat
    33481 tccgctcaaa aatcagtaca acacccgagg gaaaagggga tgcaacgcgg gttgcatgct
    33541 ccccatttgc ttagcggaag agtttccctt tcagcagttc catgcctgcg gaaagcagat
    33601 cgttattggc ttgtgctgac acttcacctt gcggtgagag cgcatcaata atcttcggca
    33661 attgctctgc cagtaaactg gaagccgtac tggtatccac gccaagtttt tgcccgagat
    33721 cggacaccgc atttgtgccg agcgccgact ccagttgctc gccgctaact gattgatttc
    33781 tctgttgatt actcaaccag gttgagagaa tggcccctaa gccgccactt tgcagttttt
    33841 ccagcagcac ctgaatgcca ccctgctcct caacccaact taaaatcgcc tgatatttcc
    33901 ccgcatcgcc tttcagaaag gcaccgacaa cttcatcaaa aagccccatg ataatcacct
    33961 gtaaagcgtt acgtgttgac ccaaaaagta tagatctgcg ggatgataat tgcggattgc
    34021 agaaataaaa agggcggaga tgatctccgc ccttttctta tagcttcttg ccggatgcgg
    34081 cgtgaacgcc ttacccggcc tacaaaatca tgcaaaatca atatattgca ggagtctcgt
    34141 aggactaata agcgtagcgc atcaggcacg ctcgcatggt taacgccatt aaatatcaat
    34201 attcgccgct ttcagggcgt tctcttcaat aaacgcacga cgcggttcta cggcgtcgcc
    34261 catcagcgtg gtgaacaact ggtcagcggc aatcgcatcc ttaacggtaa cgcgcagcat
    34321 acgacggctt tccgggtcca tggtggtttc ccacagctgt tccgggttca tctcgcccag
    34381 acctttataa cgctggatgg agaggccacg acgggactct ttcaccagcc agtccagcgc
    34441 ctgctcgaag ctggctaccg gctgacgacg ctcgccacgt tcgataaacg catcttcttc
    34501 cagcaagcca cgcagtttct cacccagcgt gcagatacga cgatattcgc caccggtgat
    34561 aaactcgtga tccagcggat agtcagtatc cacaccgtgg gtacgcacgc gaacaatcgg
    34621 ctcgaacagg ttttgttcgg cattggtgtg gacatcaaac ttccactggc tgccgtgctg
    34681 ttctttgtcg ttcagttcgc tgaccagcgc gttcacccag cgggtaacgg tctgctcatc
    34741 agaaaggtca gcttccgtca acgtcggctg atagataagc tctttcagca ttgctttcgg
    34801 ataacgacgc tccatgcggt tgatcatttt ctgcgtcgcg ttgtactcag ataccagttt
    34861 ctctaacgct tcgccagcca atgccggtgc actggcgttg gtgtgcagcg ttgcgccgtc
    34921 cagcgcgata gagatctggt actgatccat cgcttcgtcg tctttaatgt actgttcctg
    34981 cttgcctttc ttcactttgt acagcggcgg ctgagcgatg tagacgtgac cgcgttcaac
    35041 gatttccggc atctgacgat agaagaaggt caacagcagc gtacgaatgt gcgagccgtc
    35101 gacgtccgca tcggtcatga tgatgatgct gtgataacgc agtttgtccg ggttgtactc
    35161 gtcacgaccg ataccacagc caagcgcggt gataagcgtc gccacttcct gagaagagag
    35221 catcttatcg aagcgcgctt tctcgacgtt aaggatttta cccttcagcg gcagaatcgc
    35281 ctggttcttg cggttacgcc cctgcttcgc agagccgccc gcggagtccc cttccaccag
    35341 gtacagttcg gaaagcgccg gatcgcgttc ctggcagtct gccagtttgc ccggcaggcc
    35401 agctaagtcg agcgcacctt tacggcgggt catttcacgc gcgcgacgcg ccgcttcacg
    35461 ggcacgggca gcatcgataa ttttgccaac cacgattttc gcatcagttg ggttttccag
    35521 caggtattcc gccagcagtt cgttcatctg ctgttcaacc gccgatttca cctcagaaga
    35581 aaccagtttg tctttggtct gggaggagaa tttcgggtcc ggcactttta cggaaacgac
    35641 cgcaatcagg ccttcacgcg catcgtcacc ggtggcgctg actttggctt ttttgctgta
    35701 gccttctttg tccatgtagg cattcagggt acgggtcatc gccgcacgga agcctgccag
    35761 gtgagtaccg ccgtcacgct gcggaatgtt gttggtaaag cagtagatgt tttcctggaa
    35821 gccatcgttc cactgcagcg ccacttcaac gccaataccg tctttttcgg tggagaagta
    35881 gaagatattt gggtggatcg gcgttttgtt cttgttcaga tattcaacga acgctttgat
    35941 gccgccttca tagtggaagt ggtcttcttt gccgtcacgc ttgtcgcgca gacgaatgga
    36001 aacgccggag ttgaggaacg acaactcacg cagacgtttc gccagaattt catattcgaa
    36061 ctcggtcaca ttggtgaagg tttcgaggct gggccagaaa cgcaccatgg tgccggtttt
    36121 ttcagtctcg ccggtaaccg ccagcggagc ctgcggtaca ccgtgttcgt agatctgacg
    36181 gtgaatttta ccctcgcgct gaataaccag ctccagtttt tgcgacaggg cgtttactac
    36241 cgaaacacca acgccgtgca gaccgccgga cactttatag gagttatcgt caaatttacc
    36301 gcctgcgtgc agaacggtca tgatcacttc cgccgccgat acgccctctt ccgggtgaat
    36361 accggtcgga atgccgcgcc cgtcatcctg tacagagaca gagttatcgg cgtgaatggt
    36421 gacgataatt tctttacagt gacccgcgag cgcttcgtcg atagcgttat ctaccacctc
    36481 gaataccatg tggtgcagac cggtgccgtc atccgtgtcg ccgatataca tacccgggcg
    36541 cttacgcacc gcatccagcc ctttcaggac tttgatactg gaggagtcat aagaattcga
    36601 catcaacgtt tctcgctcat ttatacttgg gttaatccgt tattttaccc ttttccacgg
    36661 taaacatctt cgaattttcg tccgacatgt ctataacgtg ttcagcactg atcgcgctga
    36721 caaagacctg tgattgcgtc gcttttaagc ggctggcaag caacccgcga cgctcatcat
    36781 caagctcaga ggcaaaatca tctatcaggt agagacaccg ccgcccgctt tcacgagtga
    36841 ggaactctcc ttgcgccaga cgtaaggcgc acatcaacag cttaagctgc ccacgcgata
    36901 aggtatcttc caccggcgca ccgtcggcgc gaatgcgtaa gtccgcttta tgcgggccat
    36961 gcgcggtgta ggttagctgg cgatcgcgtt caaaattacg ttccagcacc tcagcgtact
    37021 ctgtctcttt ctcccagccg cgctggaaag agaaagtcag agaaaactca gggagaaatt
    37081 gcttacaggt atcatccata tcggccgcga taccggcgct atactccgcg cgccaggtgc
    37141 tgatttgctc cgccagcggg atcagttctt tatcccacgg gcgtagctgt tcgtaacgag
    37201 tcacctggcg cagcgccgca ttgcgctgct tgagcaatcg cttgagattg ctccaggcgg
    37261 tgaaaaatcc aggttcgttg tgaaagcatc cccagtcgag gaatgctctt ctgtatttgg
    37321 ggccgccgtt gagtaaagta aacccttctg gcgttatcaa ctgcatcggc atcaggtgcg
    37381 ccagttccgc gactttatgc ccgtccgtac cgtcaatacg gactttgctg tcgccctgtt
    37441 tgtctttggt taagccaatc gccgtctcgc gctcttcgcc ctgtaatcgc ccgtggagaa
    37501 caaatgcctc ctgctcatgg cgaatgacgc gaccaatctg caaactgcga aacgcccgac
    37561 catggccgag cgtatagatg gcttccagca cgctggtttt gccactgccg ttggcaccta
    37621 ccagaaagtt aaagccggga gataaagcga gatccgcggt ttcaatattg cggaaatcgc
    37681 ggatcaacaa gcgggtgagg gacattacag tctcattggc atgacaacat aggcagccga
    37741 ctgtgatgcg gcatcttcaa tctgcacgct ggaaaccgaa tcggtcagca tcatgcggac
    37801 gttttcgcat ttcagcgcgt tcagaacatc cagcacatag ctgacgttga agccgatttc
    37861 catctccgca ccgctatagg taacgtcgag gatctcttcc gcttcttcct gttccgggtt
    37921 gttggcagta attttcagct ggttttcgct gacatacagg cgcacaccgc ggaatttctc
    37981 gttagagaga atcgccgcgc gagcaaacgc ctgcttgagc agatcgcagc ctgcttccag
    38041 atgtttgtcc gggttcttcg gcagaacgcg acggtaatcc gggaagcgac catccaccag
    38101 tttcgaggtg aaaataaagt cgccaacgtg ggcacgaatg ttattgctgc caatctgcac
    38161 acgcagcaga ttgtcgccgc cgtcgagcat acgcatcagt tcaatcacgc ctttacgcgg
    38221 tacgatcacc gaatggcttg gcaaagattg accaattggc attgaacaga ccgccagacg
    38281 gtggccgtcg gttgccacgg tgcgcagttc ttcaccttcg gtttcaaaca gcataccgtt
    38341 taagtaatag cgaacatcct gatgcgccat cgaaaactgg gttgcttcaa tcagacgctt
    38401 catcgtcgcc tgcggcaggg taaattcgac ttcgctctgc cagtcatcga ggttcgggaa
    38461 atccgccgct ggcagggtag acagcgaaaa acggctacgc ccggagcgca ccagcatccg
    38521 ttcaccttcc agctgcacgg caatttccgc gccttcaggc agaccacggc agatatcaaa
    38581 gaatttgcgc gccggaacgg tcgtcgctcc tggctcgtgt ggctgaacca gcgcaacgcg
    38641 tgccaccatt tccatctcga gatcagtacc ggtcagcgac aacgtaccgt cagcaacctg
    38701 taacagcaga ttaccgagaa tcggtagcgt aggacgacca cctaacggac cgctcacctg
    38761 ttgtagcggt tttaataaat gctcacgttc tacggtaaat ttcataggtt tacgatgaca
    38821 atgttctgat taaatttgaa aaatcttctt tgatatcgtg gctctcttca cgcaactgct
    38881 cgatcttacg gcaggcatga agcaccgtcg tgtggtcacg cccaccaaac gcatcgccaa
    38941 tctctggcag actgtggtta gtcagctctt tcgctagcgc catcgccatc tggcgcggac
    39001 gcgccaccga gcgggatcga cgcttggaaa ggagatccgc gactttgatc ttgtagtact
    39061 ccgccaccgt cttctgaata ttgtcgatgg tgaccagttt ttcctgcaat gccagcaagt
    39121 cgcgcagcgc ctcacgcacg aagtcgatgg tgatcgcccg tccggtaaag ttggcgttag
    39181 caataacgcg gttcagcgcc ccttccagct cacgtacgtt agatcgtaga cgcttggcga
    39241 taaagaacgc cacttcaccc ggcaaacgaa tgtcattttc gtcggccttt ttcatcagga
    39301 tcgccacacg ggtttccagc tctggcggtt cgatcgccac agtcagtccc caaccgaagc
    39361 gggatttcaa acgatcctca acgccgttga tctctttcgg atagcgatcc gaggtgagaa
    39421 tgatctgttg attaccttcc agcagggcgt tgaaggtgtg gaaaaactct tcctgagatc
    39481 gttctttatt agcaaaaaac tgaatatcgt cgatcagcag tgcatctacg gaacggtagt
    39541 agcgtttaaa ctcttcgatc gcgttgtttt gcagggcttt aaccatgtcc tgaacaaagc
    39601 gctcggagtg catataaacc actttggcat tcggcttgcg cgccataatg ccgttaccca
    39661 ccgcatgcag caggtgagtt ttacccagac ccgtgccgcc ataaaggaac aacgggttat
    39721 aggcaccgcc agggttatcc gccacctggc aagccgccgc gcgcgccagt tggttagatt
    39781 taccttcaac gaagttatca aacgtgtgtt tgacgtttac gttagaacga taggtcggtt
    39841 ctgccggggc cgggacgtta tcccaacctg agcgcgtaga aggcgcagca cgttgcggct
    39901 gcgtttgcgc cacctgtgca ggggccgcga cgttgctcgt cactgccgct tgtggcgttt
    39961 gcgtcaccgg tttggtgccg acttcaaaac gcagctgtgg ggcatccgct ccgcagaaac
    40021 tggttagcag tccattgata ttattaaggt acttgtcccg tacccaatcg aggacaaaac
    40081 ggtttggcgc gtacagggcc agcgtgttat cgctcagttc cgcctgcaat gggcgtatcc
    40141 acatactgaa ttctgtggct ggtaactcat cctgcaatcg ggcaagacac tgctgccaaa
    40201 gcgaaagtga cacggcggac tccactcgaa caaaagtcga taatgactaa ggctgaaaca
    40261 ttcatgattg ttgacgtacg tcgaaaagac cctgcttgag ggtgacgcac gaaccgctgt
    40321 ctgcggttat atgcccgatc aagatcctgc aaaacgatcg ggaccgcgga tcatagccta
    40381 aactgcgcaa gagatcttct gtttctcaca gattttccct atttatccac aggactttcc
    40441 agaactcgct aagtgtaaac gatcctgccg cgaggcgggc acgatttacg ccgcatattg
    40501 gaaaatttaa tgaccataga caaaaattgg cttaatcgat ctaataaaga tccaggacga
    40561 tccttgcgct ttacccatca gcccgtataa tcctccaccc ggcgcgccat gctggtttcc
    40621 actggtgtga ggtcgtacat tttccctgcg aaaaggtgcg gaaaagcgcg gtaaataagg
    40681 aaagagaatt gactccggtg tgtacaatta ttacaatccg gcctctttaa tcacccatgg
    40741 cttcggtgtc catcgtttca tttttcggcg gatatccaat aaagccattg aatttattca
    40801 agtttaggta gaaatcgcca tgaaacgcac ttttcaaccg tctgtactga agcgcaaccg
    40861 ttctcacggc ttccgtgctc gtatggctac taaaaatggt cgtcaggttc tggcacgtcg
    40921 tcgtgctaaa ggccgcgctc gtctgaccgt ttctaagtaa taaagctaac ccctgagtgg
    40981 ttaagctcgc atttcccagg gagttacgct tgttaactcc cagtcaattc acattcgtct
    41041 tccagcagcc acaacgggct ggcacgccgc aaattaccat tctcggccgc ctgaattcgc
    41101 tggggcatcc ccgtatcggt cttacagtcg ccaagaaaaa cgttcgacgc gcccatgaac
    41161 gcaatcggat taaacgcctg acgcgtgaaa gcttccgtct gcgccaacat gaactcccgg
    41221 ctatggattt cgtggtggtg gcgaaaaaag gggttgccga cctcgataac cgtgctctct
    41281 cggaagcgtt ggaaaaatta tggcgccgcc actgtcgcct ggctcgcggg tcctgatagc
    41341 cctcattcgg gtctatcaac gcctgattag tccgctactc gggccgcatt gtcgtttcac
    41401 tccaacctgt tcaagctacg gaattgaggc attgcgcagg tttggagtga taaaaggcag
    41461 ttggttgacg gtgaaacgcg tattaaaatg ccacccttta caccctggtg gtgacgatcc
    41521 cgtcccgccc ggaccatttg ataccagaga acactaacga tggattcgca acgcaatctt
    41581 ttagtcatcg ctttgctgtt cgtgtctttc atgatctggc aagcctggga gcaggataaa
    41641 aacccgcaac ctcaggccca acagaccacg cagacaacga ccaccgcagc gggtagcgcc
    41701 gccgaccagg gcgtaccggc cagtggccag gggaaactga tctcggttaa gaccgacgtg
    41761 cttgatctga ccatcaacac ccgtggtggt gatgttgagc aagctctgct gcctgcttac
    41821 ccgaaagagc tgaactctac ccagccgttc cagctgttgg aaacttcacc gcagtttatt
    41881 tatcaggcac agagcggtct gaccggtcgt gatggcccgg ataacccggc taacggcccg
    41941 cgtccgctgt ataacgttga aaaagacgct tatgtgctgg ctgaaggtca aaacgaactg
    42001 caggtgccga tgacgtatac cgacgcggca ggcaacacgt ttaccaaaac gtttgtcctg
    42061 aaacgtggtg attacgctgt caacgtcaac tacaacgtgc agaacgctgg cgagaaaccg
    42121 ctggaaatct cctcgtttgg tcagttgaag caatccatca ctctgccacc gcatctcgat
    42181 accggaagca gcaacttcgc actgcacacc ttccgtggcg cggcgtactc cacgcctgac
    42241 gagaagtatg agaaatacaa gttcgatacc attgccgata acgaaaacct gaacatctct
    42301 tcgaaaggtg gttgggtggc gatgctgcaa cagtatttcg cgacggcgtg gatcccgcat
    42361 aacgacggta ccaacaactt ctataccgct aatctgggta acggcatcgt cgctatcggc
    42421 tataaatctc agccggtact ggttcagcct ggtcagactg gcgcgatgaa cagcaccctg
    42481 tgggttggcc cggaaatcca ggacaaaatg gcagctgttg ctccgcacct ggatctgacc
    42541 gttgattacg gttggttgtg gttcatctct cagccgctgt tcaaactgct gaaatggatc
    42601 catagctttg tgggtaactg gggcttctcc attatcatca tcacctttat cgttcgtggc
    42661 atcatgtacc cgctgaccaa agcgcagtac acctccatgg cgaagatgcg tatgctgcag
    42721 ccgaagattc aggcaatgcg tgagcgtctg ggcgatgaca aacagcgtat cagtcaggaa
    42781 atgatggcgc tgtacaaagc tgagaaggtt aacccgctgg gcggctgctt cccgctgctg
    42841 atccagatgc caatcttcct ggcgttgtac tacatgctga tgggttccgt tgaactgcgt
    42901 caggcaccgt ttgcactgtg gatccacgac ctgtcggcac aggacccgta ctacatcctg
    42961 ccgatcctga tgggcgtaac gatgttcttc attcagaaga tgtcgccgac cactgtgacc
    43021 gacccgatgc agcagaagat catgaccttt atgccggtca tcttcaccgt gttcttcctg
    43081 tggttcccgt caggtctggt actgtactat atcgtcagca acctggtaac cattattcag
    43141 cagcagctga tttaccgtgg tctggaaaaa cgtggcctgc atagccgcga gaagaaaaaa
    43201 tcctgattcg gtgagttttc gctaaaataa gggcggtcag ttgaccgcct tttttctttt
    43261 cgtagggcgg ataagcaccg cgcatccgcc acacaaagca acaggaacat catgagcgat
    43321 aatgacacta tcgtagccca ggccacgcct ccgggacgtg gcggcgttgg catcctgcgc
    43381 atctccggct tcaaagcccg tgaagttgcc gaaaccgtgc tgggtaaact gcctaagccg
    43441 cgctacgccg attatcttcc gtttaaagac gccgacggca gcgtgctcga tcaggggatt
    43501 gcgctatggt tccctggccc gaactcgttc accggcgaag atgtgctgga actgcaaggt
    43561 catggcggtc cggtgatcct cgacctgctg ttaaaacgca ttctgaccat tcccggcctg
    43621 cggattgctc gccctggtga gttttccgaa cgcgcgtttc ttaacgataa acttgactta
    43681 gcccaggccg aggcgattgc cgatcttatc gacgccagtt cggaacaggc ggcccgttcg
    43741 gcacttaact cgctgcaagg cgcattctcc gcacgggtta atcatctggt agaagccctc
    43801 acccacttgc gcatttacgt cgaagcggca attgatttcc ctgatgaaga gatcgatttc
    43861 ctctccgacg gcaaaattga agcccagctt aatgacgtga tggccgatct cgatgcagtg
    43921 cgtgctgaag cacgtcaggg tagtttgttg cgcgaaggga tgaaagtagt gattgccgga
    43981 cgtcctaatg ccggtaaatc gagcctgtta aacgcgctgg cgggccgtga agcggcaatc
    44041 gtaaccgata tcgccggaac cacgcgtgac gtgctgcgtg agcatatcca cattgacgga
    44101 atgccgctgc atatcatcga cactgccggg ctacgtgagg ccagtgacga agtggaacgt
    44161 attggtatcg agcgcgcgtg gcaggaaatt gaacaggccg accgcgtgct gtttatggtc
    44221 gatggcacca cgacagacgc cgttgatccg gcagagatct ggccggaatt tatcgcccgt
    44281 ctgcccgcga aactgccgat caccgtggta cgcaataaag ccgatatcac cggcgaaacg
    44341 ctgggaatga gtgaagtgaa cggtcacgcg ttaattcgtc tctcggcaag gactggtgaa
    44401 ggcgtggacg tgctgcgtaa ccatctcaaa cagagcatgg gctttgacac caatatggaa
    44461 ggcggcttcc tggcgcgccg tcgccactta caggcgctgg aacaggcagc ggagcatttg
    44521 cagcaaggta aagcgcaact gttgggagcc tgggcaggtg aactgctggc ggaagagttg
    44581 cgcctggcac agcagaactt aagcgaaatc accggggaat ttacttcaga cgacctgctg
    44641 gggcggattt tctccagctt ctgtattggt aagtaaccgc gcttacgaag ccgtattctg
    44701 actgtcagat gcggcttcgc ttcattgtta gcactcctgt tatgcctcaa ccctttttta
    44761 aacattaaaa ttcttacgta atttataatc tttaaaaaaa gcatttaata ctgctccccg
    44821 aacgattgtg attcgattca catttaaaca atttcagaat agacaaa
//
LOCUS       DAZSNM010000012        40488 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZSNM010000012 DAZSNM010000000
VERSION     DAZSNM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40488
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.012"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(88..1338)
                     /gene="icd"
                     /locus_tag="ACHW42_000566"
     CDS             complement(88..1338)
                     /gene="icd"
                     /locus_tag="ACHW42_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR8942524.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1510..2163
                     /gene="rluE"
                     /locus_tag="ACHW42_000567"
     CDS             1510..2163
                     /gene="rluE"
                     /locus_tag="ACHW42_000567"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR8942525.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2173..2634
                     /gene="nudJ"
                     /locus_tag="ACHW42_000568"
     CDS             2173..2634
                     /gene="nudJ"
                     /locus_tag="ACHW42_000568"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR8942526.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2688..3794
                     /gene="mnmA"
                     /locus_tag="ACHW42_000569"
                     /gene_synonym="trmU"
     CDS             2688..3794
                     /gene="mnmA"
                     /locus_tag="ACHW42_000569"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR8942527.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3830..4471
                     /gene="hflD"
                     /locus_tag="ACHW42_000570"
     CDS             3830..4471
                     /gene="hflD"
                     /locus_tag="ACHW42_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR8942528.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4475..5845
                     /gene="purB"
                     /locus_tag="ACHW42_000571"
     CDS             4475..5845
                     /gene="purB"
                     /locus_tag="ACHW42_000571"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR8942529.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6014..6685
                     /gene="phoP"
                     /locus_tag="ACHW42_000572"
     CDS             6014..6685
                     /gene="phoP"
                     /locus_tag="ACHW42_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR8942530.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6685..8145
                     /gene="phoQ"
                     /locus_tag="ACHW42_000573"
     CDS             6685..8145
                     /gene="phoQ"
                     /locus_tag="ACHW42_000573"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR8942531.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     SWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8942..9223)
                     /locus_tag="ACHW42_000574"
     CDS             complement(8942..9223)
                     /locus_tag="ACHW42_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942532.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9237..10898)
                     /locus_tag="ACHW42_000575"
     CDS             complement(9237..10898)
                     /locus_tag="ACHW42_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR8942533.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10882..11208)
                     /locus_tag="ACHW42_000576"
     CDS             complement(10882..11208)
                     /locus_tag="ACHW42_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR8942534.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11264..11446)
                     /locus_tag="ACHW42_000577"
     CDS             complement(11264..11446)
                     /locus_tag="ACHW42_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942535.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11430..11870)
                     /locus_tag="ACHW42_000578"
     CDS             complement(11430..11870)
                     /locus_tag="ACHW42_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR8942536.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11870..12166)
                     /locus_tag="ACHW42_000579"
     CDS             complement(11870..12166)
                     /locus_tag="ACHW42_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFR8942537.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12163..12501)
                     /locus_tag="ACHW42_000580"
     CDS             complement(12163..12501)
                     /locus_tag="ACHW42_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFR8942538.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12498..13709)
                     /locus_tag="ACHW42_000581"
     CDS             complement(12498..13709)
                     /locus_tag="ACHW42_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR8942539.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13711..14283)
                     /locus_tag="ACHW42_000582"
     CDS             complement(13711..14283)
                     /locus_tag="ACHW42_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFR8942540.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14323..15480)
                     /locus_tag="ACHW42_000583"
                     /pseudo
     CDS             complement(14323..15480)
                     /locus_tag="ACHW42_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15871..15945)
                     /locus_tag="ACHW42_000584"
                     /pseudo
     CDS             complement(15871..15945)
                     /locus_tag="ACHW42_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15955..16260)
                     /locus_tag="ACHW42_000585"
     CDS             complement(15955..16260)
                     /locus_tag="ACHW42_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942541.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16257..16535)
                     /locus_tag="ACHW42_000586"
     CDS             complement(16257..16535)
                     /locus_tag="ACHW42_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942542.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(16824..18572)
                     /locus_tag="ACHW42_000587"
     CDS             complement(16824..18572)
                     /locus_tag="ACHW42_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFR8942543.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18569..18868)
                     /locus_tag="ACHW42_000588"
     CDS             complement(18569..18868)
                     /locus_tag="ACHW42_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR8942544.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(18874..19107)
                     /locus_tag="ACHW42_000589"
     CDS             complement(18874..19107)
                     /locus_tag="ACHW42_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942545.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19109..19329)
                     /locus_tag="ACHW42_000590"
                     /pseudo
     CDS             complement(19109..19329)
                     /locus_tag="ACHW42_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19322..19543)
                     /locus_tag="ACHW42_000591"
     CDS             complement(19322..19543)
                     /locus_tag="ACHW42_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942546.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19533..19733)
                     /locus_tag="ACHW42_000592"
     CDS             complement(19533..19733)
                     /locus_tag="ACHW42_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFR8942547.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            19863..20042
                     /locus_tag="ACHW42_000593"
     CDS             19863..20042
                     /locus_tag="ACHW42_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942548.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20167..20496
                     /locus_tag="ACHW42_000594"
     CDS             20167..20496
                     /locus_tag="ACHW42_000594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942549.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20546..20734)
                     /locus_tag="ACHW42_000595"
     CDS             complement(20546..20734)
                     /locus_tag="ACHW42_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8942550.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21109..22338)
                     /locus_tag="ACHW42_000596"
     CDS             complement(21109..22338)
                     /locus_tag="ACHW42_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8942551.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22587..23708
                     /gene="roxA"
                     /locus_tag="ACHW42_000597"
                     /gene_synonym="ycfD"
     CDS             22587..23708
                     /gene="roxA"
                     /locus_tag="ACHW42_000597"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR8942552.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(23757..24983)
                     /gene="pepT"
                     /locus_tag="ACHW42_000598"
     CDS             complement(23757..24983)
                     /gene="pepT"
                     /locus_tag="ACHW42_000598"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR8942553.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25233..26369
                     /gene="potA"
                     /locus_tag="ACHW42_000599"
     CDS             25233..26369
                     /gene="potA"
                     /locus_tag="ACHW42_000599"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR8942554.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26353..27210
                     /gene="potB"
                     /locus_tag="ACHW42_000600"
     CDS             26353..27210
                     /gene="potB"
                     /locus_tag="ACHW42_000600"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR8942555.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27207..28001
                     /gene="potC"
                     /locus_tag="ACHW42_000601"
     CDS             27207..28001
                     /gene="potC"
                     /locus_tag="ACHW42_000601"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR8942556.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            27998..29044
                     /gene="potD"
                     /locus_tag="ACHW42_000602"
     CDS             27998..29044
                     /gene="potD"
                     /locus_tag="ACHW42_000602"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR8942557.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29200..30021)
                     /gene="cobB"
                     /locus_tag="ACHW42_000603"
     CDS             complement(29200..30021)
                     /gene="cobB"
                     /locus_tag="ACHW42_000603"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR8942558.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30037..30948)
                     /gene="nagK"
                     /locus_tag="ACHW42_000604"
     CDS             complement(30037..30948)
                     /gene="nagK"
                     /locus_tag="ACHW42_000604"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR8942559.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(30977..32221)
                     /gene="lolE"
                     /locus_tag="ACHW42_000605"
     CDS             complement(30977..32221)
                     /gene="lolE"
                     /locus_tag="ACHW42_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR8942560.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32221..32922)
                     /gene="lolD"
                     /locus_tag="ACHW42_000606"
     CDS             complement(32221..32922)
                     /gene="lolD"
                     /locus_tag="ACHW42_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR8942561.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(32915..34114)
                     /gene="lolC"
                     /locus_tag="ACHW42_000607"
     CDS             complement(32915..34114)
                     /gene="lolC"
                     /locus_tag="ACHW42_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR8942562.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34376..35449
                     /locus_tag="ACHW42_000608"
     CDS             34376..35449
                     /locus_tag="ACHW42_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR8942563.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35577..39023
                     /gene="mfd"
                     /locus_tag="ACHW42_000609"
     CDS             35577..39023
                     /gene="mfd"
                     /locus_tag="ACHW42_000609"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR8942564.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39166..40128
                     /gene="ldtC"
                     /locus_tag="ACHW42_000610"
     CDS             39166..40128
                     /gene="ldtC"
                     /locus_tag="ACHW42_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFR8942565.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40211..40423)
                     /locus_tag="ACHW42_000611"
     CDS             complement(40211..40423)
                     /locus_tag="ACHW42_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFR8942566.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         9517 a        10699 c        10022 g        10250 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      421 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      481 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat tcgggttttt
      541 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac gcgccagctg
      601 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      661 ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      721 ggtgccttct tcagaacacg gcttaatacc gataccgcaa tgttccggga agcgaatttt
      781 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtcagc
      841 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      901 cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg gacgcaggca
      961 gatgtagaga tccagttcct gacgcagggc aacgttgaga gaacgaatac cgccaccaac
     1021 cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1081 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1141 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1201 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1261 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1321 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1381 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1441 atttgtaatt gacttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1501 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1561 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaaca tgaaaaccag
     1621 cccacgcgtg tgatcctctt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1681 accggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgc
     1741 cttgaccgcg atagcgaagg gttgctggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1801 ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga aggtattccc
     1861 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1921 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1981 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2041 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2101 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2161 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2221 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2281 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2341 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2401 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2461 catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt acaggcgtca
     2521 aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg gcaacgttat
     2581 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2641 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2701 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2761 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2821 gtgaggaata ttgcacagca gcagcggatc tggctgatgc ccaggcggtc tgcgacaagc
     2881 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2941 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaagg
     3001 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3061 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3121 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3181 aaagcctgtt cccggtaggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3241 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3301 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3361 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3421 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3481 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3541 tcgggttgat tgcccagcag ctgcactggg tcgatcgcga accattcacc ggcactatgc
     3601 gttgcacggt aaaaacccgc tatcgccaga ccgacattcc gtgcaccgtc aaggcgctgg
     3661 acgacgatcg cattaaagtg attttcgatg agccggttgc cgccgtgacg ccgggccagt
     3721 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3781 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3841 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3901 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3961 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4021 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt tgccagggct taaacgccga
     4081 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4141 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4201 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4261 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4321 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4381 cggcggcgga cgtctacaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4441 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4501 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4561 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4621 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4681 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4741 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4801 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4861 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     4921 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4981 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5041 ccgtatggag cgccagcacc gccagcttaa ccaggtggag atcctcggca aaatcaacgg
     5101 cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact ggcatcagtt
     5161 cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta cccagattga
     5221 accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5281 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5341 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg acttcgaaaa
     5401 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5461 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5521 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5581 gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg cagaaccaat
     5641 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagcttac
     5701 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5761 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5821 cacgatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga tgctgtccgg
     5881 cctgcttatt aagattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     5941 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaataca
     6001 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6061 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6121 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6181 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaac gatgtttcac tgccgattct
     6241 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6301 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6361 gcggcgtaat agcggtctgg cttcacaggt catttcgctc cccccgtttc aggttgatct
     6421 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6481 cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt cgttaatgct
     6541 acaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6601 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtc attaccaccg ttcgcggcca
     6661 gggctacctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6721 ctgcgggtac gttttctgtt ggcaacggca gcggtagtac tggtgctttc gcttgcctac
     6781 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6841 cgtggcgaga gcaatctgtt ctataccctt gcgcagtggg aaaacaataa gttgcatgtc
     6901 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6961 gggcagcttt tatgggcgca acgtgacgtg tcctggctga tgaaaatgat ccagcctgac
     7021 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7081 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7141 gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg gatgccaaaa
     7201 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7261 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7321 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7381 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7441 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7501 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7561 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7621 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7681 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7741 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7801 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7861 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7921 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7981 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8041 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8101 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccttttacc
     8161 actacatcaa gtcattgatt cgatcaaaca attttcattg aaaaagtgcc aaaaatcgca
     8221 aattgactac accattaact acaccgttcg gtgcactgta tgaaacaacg tggaacaaat
     8281 agacacaaga aatatacagg cgggtaatct ttccaggggg aagcgcacca attcatgagg
     8341 ggcgttaatg tcgatatggg gatccccata acggggctac cggatttttt ttccggttaa
     8401 cctttaatca ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt
     8461 gcaccttttc caccaatggc ggaaaagctc caaccgtaac gatgatcgct atggttgcct
     8521 cgatggtctg cgtggtcatt atgaccgttc agccagtgac cttaatcaga atttttcgct
     8581 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatgaac
     8641 ggttaaaccg tctttttgtc acggtgtgtc actggttgtc actctccgtc acgattgagg
     8701 tggtggtcat tctgcccgac cgcgcatctt tgcgctatcg ggaatatcaa acagttacct
     8761 cgccgacttc ctcaaattga ggtttccgaa aatatcagag cgggttagcg attaagctga
     8821 cgagtaatta aactgcgaaa atttcgtagt ttctgaatat attccggcct gctggttaac
     8881 agtcctcaga tgtgagggat gtagccagcg caattttgcg ctttgcttta atctcagtga
     8941 gttaccgccg taaccgctcc ggcttcttcc agtggtacgt gattttctcc ttctcccgat
     9001 acagttcaac gcggcgattg taggccagca tttccagaac gcggatccgt atgtcgcgca
     9061 tatccgcatc attaagctgg ataccatcac ggcgcatcac ctcagcaaca acacgcgcat
     9121 aattttcggc tgtcacgctg tccggctgcg tggtctgttc gtcagcctgc tggctgattc
     9181 cagagacgcg gcggattaat cgcagtattt cggcttctgt catgctgctg acctcattac
     9241 accccgctaa attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc
     9301 tcatgctctg cctggaatgt accaaacgcc atcagcgccg ctacgctcgg gtctatcttg
     9361 ttcggtgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg
     9421 ttactcatcg accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca
     9481 aaaacttcga acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc
     9541 acctcaaaac cagccccctg taattgcgtc ctgagatgcg tggcgttcca cgtatcaaag
     9601 cccaccagcc tgatattaaa tttctccgca tcctgcatga tgtcatctct gatccggtcg
     9661 taatcaatgc agtcacctgg cgttgtgcgt atccagcccg ctttagccca ctggcgatag
     9721 acagcgcggt ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa
     9781 atcagcataa tggttttacc gaccggaaag gcatagcata cgctggaaat atcgctggtt
     9841 gatgataagt ccagccccgc gtaacactcc tgaccgtgta aatcttcctc cgtgaacgtt
     9901 ccggcacact cagcccatgc accattaccc atccacgggg tagtcccctg acaccagata
     9961 ttaaatcgct ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg
    10021 gttgaggcca gtttttcacg atccacggaa acatcaatat tagggttcgc ctttatccac
    10081 atcgccggat catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa
    10141 tcgttaacct cttcgccgtc caggatctgg cagcaataat catagtgctg tttacaggct
    10201 gaaacgacgt tactgcccga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc
    10261 atccccagct caagcgcgga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg
    10321 atggaaagac tggggtttgt cccctcgatg gttgccgctt ttgctgccag cggctttaac
    10381 aggctgttgc ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac
    10441 ggtcgggata aaaggcacat ctgacgcgca ttatcaaaca cgatccgcgc ctggtcacgg
    10501 ctcaccgctg cggtgtagat atcctgctga ccgttctcca taatcagaaa ccagttagcc
    10561 agaatcgcgg cggtcgtgga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg
    10621 tatttgcgcc gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc
    10681 tgccacggct ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc
    10741 ctggaaaagg caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta
    10801 aggtccgaaa agtaccgttt aacagcctgt tttaaccgtt tacaggccgg aattttgccc
    10861 gtttttacgt cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc
    10921 cggtggattt ttacggcggc ttatcgggtc aaaaccgagc aaggaggcca tttttatcat
    10981 cactctttca gcgtcggatt ttgcgcttaa tgcggggttt ctgctctcgc cgccctgact
    11041 gttaacaatg ctgaacccgc gcgccgcaag gtctgcaacg gctttccggt aaatggagta
    11101 gttgacgcaa tacagttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc
    11161 aagctgccgc gatttttccc gccactgctt cacggcgata tcatccaggt aggcggggtc
    11221 gggtttgtcc tgaaaacgcc ccccaccccg tcatacagcc tcatcagcga ttgcggaaac
    11281 attccataac ctcgcggtca cggtcggtta atcgcttcgc tgtgatgcgt tctgcgcgtc
    11341 ctgacgcttt atctttatgc cctgtttcct gtgtcttcca tgcgtcacgc tgccttataa
    11401 gtccacggat aaggcggttt tgttcccgct cattcatcag cgtcatacat ccagttattg
    11461 cggttagcag cccgttcttc ctcttcacga aatccacctg cggcacgctt gctttttgtt
    11521 gccggatcaa gccatttcgt tttctggtta tgacacgcct ggcacaatgg ttgatggttc
    11581 cattcgggcc agaagagaac atcatcaccg ccattaatcg ggataatgtg atccaccacc
    11641 acggcgggcg tatatatccc cttctcaagg catcgcacgc ataacgggtt tttactcaga
    11701 tacatggcgc ggtatttgtc ccactgtctg gaatatccac gcgcgcggcg gtgtcctcgt
    11761 ctggcatcct ctgcacgcca tgccgcccgc ctgtgctcct cacacttgcc ggatttaacg
    11821 cgcctgttac agcctggttc tgtgcatcgt cttaatggtt gccacggcat cagtaccccc
    11881 ccacatcacg gtaagccgtc cagagtgcgc ctatcgtcat gggtacgcgt gtttttgcgt
    11941 tatccgcgac aatctggcga ttttcataca ggtgagcgat aaacatcatg cagccaatct
    12001 ttatggctgg cgtgaacgcc agcccgtcat caaagcgcct gcctatgtgt atctggcacg
    12061 cctcaagcga tgcggcaatg tatccgctga ttaactggtc ttcatcgtcg ccatcgatgc
    12121 ggcaatggag tttcacttct tccagggtga taagttcttc tgtcattttt ccgcgccctc
    12181 acgacaaaga atttcaaggc gtgtcctggc ggcatccggc agcggctgcc cgatgatatt
    12241 cagtacacgc cccgccagcg gccccgtact gacctttatc cggctggtgg cgttgatgtc
    12301 cttccggtac cgtatccaga tccttacggt accaatcgcc agctctgcgc ccgatgaaat
    12361 ggcctccttg ctgctgacca tgttcacgct tgcccagagt gtgtgaccgt cctcccacgt
    12421 ttcgagtatt tcgccggtta tatccctggt ctgtttcagg gtctgaatcg ttatcctgtc
    12481 acgcagtcgc cctatgttca ttccggtttt tctccctcgc tgatttttac ttcctgtttc
    12541 catgcctggc tgaactcatc accaccatca cgcggtgaca gcccttcacg ttcgcgggct
    12601 tcgttcggac acataacgtc ggattttatg ccgcgctcat aggtggcaaa gcgttcgcca
    12661 ggtgtggccc gtaacaggtc cgcgctgtca aactccacct gataccggat accaggtaca
    12721 ggcgaggcca ccagcaaggc gttttttatc tgctgctcaa agttcgccag ccacgggcgc
    12781 atcgtcatgg tgagaaatgc gcggcttgcc tcgctgaagt tgctgtaggt gctgttgctg
    12841 tattcctgca aaaaaatcgg cgagacgttg aacattcggg cgatgtcttc aatggtgaag
    12901 cgacgggagg ccagccattc ggcatcctga ttactcatgc ccagctgctg atagctcatc
    12961 cccccttcaa ggatgggcgt ttttccggcg tttctggccc ctttgtagcg ttccagtgcg
    13021 gctaatgcct gtttgccctt cacgccgtcc agccattcgc ctgacgtgat aacccctgcc
    13081 gccatcatgc catcacgcat cacgctcgcg ccgtggcgtt gttgggccag cccaagcccc
    13141 agcgattcgc ggcagatggt tacaggtgag cgccccataa aaccgtcatc cgtggagtaa
    13201 cgaaggtgga gaacctccca cggtaaatag ttgcggatgt ttccggtgta ggcgtcagtg
    13261 atgcagtagc gccagttatg atcacctgtc tgctccacgt tcaccgactg cggcgggtaa
    13321 ggatgtaaag ccgccggaaa accatcacgc ccccactgaa taaccgcata agcattacca
    13381 cttaacaggc agtagcggat catcattcgc ttaaactggt agggtgtctg ccacgcgttc
    13441 gggcgctcgt tgaggatgtg atcgaccgga tgagaatcaa gccattcgcg ggcctccttc
    13501 cccttctcat tgcgtaccag gtacaggtaa cacggcatgg tagccaccgc gtcagagatg
    13561 accgtgacgg cgttcatgac ggcggggagt gattccgctg tcccagatga tacgtactca
    13621 cccgcccccg tgttcgatgt gccggccagc gccataaatt catcaagcgt catgctgcgc
    13681 tgctcttttt ttcgtctgaa aggccacata tcacaccccc gctaaatccg cccaccagcg
    13741 gcgattatcc gcacgcggca ttttttcggg gtgctgctca tacagggaac ggcgggccag
    13801 ctccacgccg gaatcggggt aagccggtac cgatgtaacg gtaatttcgt acagttccgc
    13861 caccagcacg gtgcgcacgc atggatctgt tgtggtatcc catacatcct tacgggagcg
    13921 aaagccaaag ctcatccccg agatgtcccc acgcttaacc agttcgataa cgtcgcgccc
    13981 tgtactggta tccggtgggg tcagttcaaa gcgtaaccct gtttcgtcct cttccagttt
    14041 cagcgtgccg gaacgggtgc gccccagtaa catgctgtgg tcatgctcat acaggccgcg
    14101 aacgtcatta cccgccgcaa gccattcagt aaacgccccc cgctggaatt tttcgtaaaa
    14161 ctcaccccat aacagttctg aaagcttatc ccagcgaaca acgtagcccg tcagcgtacc
    14221 ggctccgctg gtggtgattt ccgatgaccg gatttccata ctcttcataa ttttttcacc
    14281 cataaaacac taaaggggct ttttagcccc ttctgtatgc tgttaatcgt cgtcttccgg
    14341 cagtgccaga atcttgatcg cgttcgaatc caccacgcca ccgcctaaat atttttgcgt
    14401 gaaaattttg atgaatcctg gttctgtcag gttgtccggt ctggtgcgaa cacctgtttc
    14461 gtgatcaaca atgtagtaac cgcgtttgaa gtcacccagg gcaataacgt tatcaggcat
    14521 aaactccaga tattcgaccg gaaggcccag caacgtatca ggatcacccg cctgtaaacg
    14581 gtcgcgccag atataatcac cgttcgcgtt cttcaccttc tgaagttttg ccgccgtcgt
    14641 ggagttaacc actcagaccg cgtttttgcg gtatttttta cgtaatgcaa atttcaggtc
    14701 gatcagcggg tccgcagatg tccacgccag agattcggaa ggtttaatta cctgcaacgt
    14761 accaaaatca cgctctttgt cgttcttctc tgcacggggt acggataaaa aaccttttgc
    14821 ttttttgtca ccgtcgccca caaccagatc gctttcttcg gtttccgtga aggtgtcgcc
    14881 aatctcaccc gtcagccatg aaaggatgtc cacatcggaa aaatccacga tttcctgtgt
    14941 ggtgcgcggg tacgcataga ccggatacag cttaatgctc acctcgttaa tctgcggggt
    15001 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
    15061 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc gacgcatggt
    15121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
    15181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca ccggtcagaa tgtagttacg
    15241 tagttctgcg gggtcagtgg tctggctgct tttacctggc ttgctgcgct cttcgtctgc
    15301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
    15361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttcagcgt tctccagcat
    15421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctctgcat
    15481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
    15541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
    15601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
    15661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
    15721 ataatttaca aaaaataaca aaaagccgga ttgctccggc tgcgtggtgt ggttctgata
    15781 ttcctactct gctaatttgc tcagccccat gtctgcgtaa gtgcgattta ctgcatttct
    15841 caggtctgcg tagttctccg ttggcggctc cggtgtgccc ttgctgctct gttttaccag
    15901 cgggtcgccc accagctgcc cgtaagctgc aatttgtgct gtcatattgc cacctcagtg
    15961 aagcgatccg gcattgttct ttttcatctc ttccatcatt ttttcagtga gcattacgca
    16021 ctgaaacccc atgtttttga tgtctacggg tttcatgcct ttgtcagtgg atatcacagg
    16081 cagaatatcc tgatacacat cgcggtcagg gtaactaagc acacaaagct cccatgatgc
    16141 cacgggtgag gatccttcgt ctgtttcaac ccaccagcct ggtgtagctg cgcttatttg
    16201 cagaatcgtg atccgtccgt tgtgcttgta aaatttttca ccaaattttg gttcgctcat
    16261 gattccacct ctccggtttt aacgttgatt gttgttacct gttccgcttc ggcaatctcc
    16321 cgctctgtca gcgtggcaaa atttgccgcc gtcgtggtca tgaatgcgct tatcagttcg
    16381 ggatgtgctt tcgcgtatcc ttcccccgcg tggcggtcta tcgttctgat tgccaccttt
    16441 aaagcgtgct ctgtcatgtc taacgcgcga tatttcggtt ctgttctgtc tctgcgtttt
    16501 ttgaatgatt tattaaattt ccctgaagtg tgcatattta tttttacccc ctcgtttaaa
    16561 aagttttgag ttgtgcctcc ccttgtctac cttatctacc ttagtggccc tcatgccagt
    16621 aatggcgctg ctttcagcgg ggtagagtgc ttttatccac tatctacccc gtgtctacct
    16681 ccctgtctga ttcaggtaaa atcaggtaga gagggtagat agtgggtaga cagtaaaaaa
    16741 aggctatcca cctaacttaa tgcactgaat taaatgtatt tttctttact caggtagaca
    16801 gggtagacag caattacaaa aaattataaa aacgcgtcgc actcgtctgt tgttattgcg
    16861 ttagtctgcg ttactccctt aactttccgc gtaatatatt catgtccgta aactttcgcg
    16921 gctggcttca tagccttgcc aaagtcattt acgtttagcg gtttgctcct gcctgcgtac
    16981 gccataaacg ccagatagac gcggtaaagg ctgtttctgg tcgtgtactt cactgaatcg
    17041 ccaccgccgc ccatcatcag gccgcgtgct tcctccagaa aattcaggaa ctggcaaaac
    17101 tcaataaccg gatccgtctg ttgctttatt gccagagctt catcaccgtc acgctgttcc
    17161 agtagtaaag cccgtgcttt ctcagagtcg gtaaagttcg ccagcaatcg gcggataatg
    17221 acagggattt cagccgcaat cttttccggt agctccctgt ctttttcggc ctcactaacg
    17281 atattgtcga aacggaaaat cacgcgacga cgtgccacac ctccggcccg ttcggtgaat
    17341 atcatcgggt tattgttggt cgccagcacc accgccctga ttacagccgt gaaacgcttt
    17401 tcgtatttcg ggttaatttc cacggggtca ccgcccgtga ttttcttgat gcccgttcct
    17461 tcgcctgtat atttcggctg gtcagccagg acgataagac gactcccgac aacctgcgca
    17521 cgtccaccag catcatcaag cgatgtcatt tcagcgctta ccgtgttctg tttccctgcc
    17581 agaaggctgg ctatgtgtgt gaatgtactt ttaccgctcc cgccgtctcc ggtggcctca
    17641 ataaacatct gccagtcgta ccggttcgcc ataatcatgt acagcgcggc acatatacgc
    17701 atcatcttgc gctggtcttt tccggctgcg tgctcaagcc atttatgaaa gtttagcgcg
    17761 ttatcgcgga tgttctcccc tggtgctggt ggcgtgtact caatgccgtt gtgcgtggtg
    17821 atccagttct ccggcgtgtg cggggaaaat tcccccgttt tcaggtcaag cgcaccattg
    17881 gcgaacggca gcaaatcgcc ggacggctcg cccattggtt cggcaataac ttttaacgct
    17941 tccacggcgt tattgattac gcgcttgctg aaagtggccc tgtgctctga atagatcacc
    18001 accatttcgc ggctaagttc cattgtgctg accggacacc ataccccgcc gcgccatacg
    18061 tgaacgattt cactttcctg atgcacacaa acgccatcaa agcgctcggc aagcagctgc
    18121 gcgcgctcac tgtctgccat ttgtgccagt tgcgtatttt tctgctccgg catggtaagc
    18181 cctgcggcaa tattttcacg ctcagcattc agatagcgcc gccagttttc acacttctgg
    18241 acgtgcattc cttcggggta aaaatttgcg tcctgaatat ctgccgccgc cagcttctga
    18301 ccaatctttt tggtctccac caaatccagt tctccggcct ggtacagcct tacgcgtttt
    18361 tttccatccg gaacaatttt cagtgcatca agttcggcaa gctgatttgg cccaagccac
    18421 acaggcggca cattatcgcc ggatgcgggg ccatcctgct cctgccattg tttcgcgtgc
    18481 gaccaggcat cactacccgc aaagataatt acctctgtgt ctttgtgttt tattccgcgt
    18541 ggctgttttt ttacgttcgg tgccagtttc attttttacc cctgaatacg ttaagcatct
    18601 tttttatttc ctgaatattg gcgcgtgctt tctccctgct ggttggttta ctgcggggcg
    18661 ctgcctgtac cagcgtgaaa tcacgccgga attgataaac aggcatcacg caatcatatt
    18721 cgtaaccttc acggcggtag gtgatgcgcc gttctgccac gcctttaatc gttaccatgc
    18781 cgccgtactg gtcgcggtaa atatcaccgc gcgtaaattt agggtgagtg ttgccactgg
    18841 cagttaagcc agaatattta agtttcatta tttttattct ccggtgtgtt gttctttata
    18901 tctgtcgtgc aatagatcta tttcttgcag ttccattatt acaggctcaa gaagcgttat
    18961 taatgccgtg gcaattcttg atttttgttt gtcgcgttca ttgtcgccaa gtgtttcaag
    19021 ccatatgcgc aatatttcca gcatgttttc actgtgagaa agtgcaagaa atgcgcggtc
    19081 tattgtttcg tggtaaatat cacgcatatt aatccccgtc cgtcgttttt cttaaaataa
    19141 cctctgtcac gaaatcagca taatcggcgg cgatattcag tacatcaagc ccgttgattt
    19201 atattctctc gtggaaagta agaaaaaagc cgctctaata agttctggca ttgacgaaag
    19261 cgcatcagcc gcatcatcag gaacgccgga aaattcctgt ttcagggaat taaaacgatc
    19321 atcacgcata acccccccca ttttcacaat cggcaacaag aatattttta gcgtcattca
    19381 gtgaccgcgt tgcgatgtat cggatacatt ccagggcgaa ctgtgtccgg tctttctgtt
    19441 cttctccctg cgcaagctct gccgtgcatt caatatcaat aagcgcgtgc atcagcgtag
    19501 tgagtgcggc ggctgctgcg ttcggtgtgg ttttattgtg cataggtttc cccctgacga
    19561 atacgggcgg cgaatacagc aacacaaccg gacgggcaac ggctacgcgc ttcgcgttcc
    19621 gtccaagcgg ttacgtggat gatttgggat tctccggcac tcagtgccag aaaacgccac
    19681 acaaaggccg tttgtgtgtg tacaaggtgt ggtatatgat ttacggcaac cataacggct
    19741 cctagtttac gttgttggtt agaagccccg ttactgctcc tacagtgcgg ggctttgctt
    19801 tttgtgttga ttgtgtgtaa tgtgtcacta cacataaata cattacctga ggtgtaatta
    19861 acgtgtcaac acacaaaaac gaaaggcgtg gtaatcctcc gttccagttc cgtttagatc
    19921 ctgacttacg ggaaaagatg gaaaaggcac aacaacagga cggcgatgat tctcttgctg
    19981 cctggataaa gcgaataatc cgcaaagaat tacagcaacg cggcatagaa cccaaaggat
    20041 aaaaactatc agcgccgtgg tgtgaggtac tacggcgcat tgctttacag gcgcacacaa
    20101 tgaccaacga agaatcaacc gaaaaaacat cacctgcacg aaaaagacga cgcagaaaga
    20161 aagagcatga atcagaacga ttcgcgccgt gttcttttgc ccttgagaag taccttaaag
    20221 aatactcatc aaaggagaga acttcgcagt tatggcaacg cactgaacca gactgattgc
    20281 attgcccacc agcctgatag cggctatcat ccccgtgctt atgtttggga tcacacacac
    20341 aaacggcgca gcgggttatc tgttcagaaa ggcggctcca tttcggggcc gcttttttta
    20401 tgcctgaaaa accccaattt tgtggttttc cagtttcacc agggcgaacg aatccccgcc
    20461 cacgttctgg cgtatattca atcttcatgg ttatagctct gtgttcagat gattgatgtg
    20521 tggcggctgc gtgccgccag cgtgattaat gaactgcctt gcagctatcc ttccaggcca
    20581 gaacctcgga taaagaccag ccaacggaac gaccgccaag tttacgacgt gatgggaatt
    20641 gtccggcctt ttccaggcgg tagcggcatg agcggctaag gcctgttagc ttttcgcatt
    20701 ctttttcacg tataaaccga tcagtgctta acactattgc cccctttcgt ttcttaaaga
    20761 gttatttcgt gttctattgc gttgggatgt gtctgattgt gtcaggatga ttcagagttg
    20821 gcaaatgttg aggccgcatg gtttacagaa agaggaataa tcaggataaa atcctttaaa
    20881 ttcatgttaa tacaaaggca taaaatattg tttcatgcct tttttctcgc tatttaaaga
    20941 gtgattcgct agtgtataaa aaatcagtaa cacattaaaa atcagctact tataaatctg
    21001 tacacttttt cacctcttgt tcgtagttgt tccgcattgt tgctcattgt tgcacgttgt
    21061 atctgttcgc atatccagta tgcgcatact gaaaaaacac gaaaaaaatt attttcttct
    21121 ggctactggt agcgtggtta cgttttcatg tgttcccgcc agtatcccta accgctccgt
    21181 ccacatatcc agcgcattgc gtttagcatc cagataacgg gaatgattat aaactcgctg
    21241 cattcctggc atctggtggc ctgtaagctg ctccacgacg tgaggatcca cgcctaaatc
    21301 gttcagcatc gttgtaaagg tgcgccggat gtcatgtagt gaccagtgag ggtgatttag
    21361 cctcctgtgc gctaatctgc cgtactgcga cacgcttgtt tcctgtttca cttcccccag
    21421 caataagccc gtgtgcctgt tctgctccac cagctgcgtg acgaacggca gtattgcttc
    21481 cggtatgggc cggaatattg ccaccttcgt tttgctgtgt tctttcggca cggtccagag
    21541 catttcggta aaatcccact cgctgatctc cgataacctc agttctaccg tccggcatcc
    21601 gaacacaatc aggaggcgga ttaacgcgat gtagtagggg gagaatattt ttttgtccag
    21661 tgcctgcaat aattcgccca gttctttggt gcttaagaca cgctcgctta tatccggttt
    21721 tttcccaacg tccgccacac tcatatcatc aagaacgttg ctgattgcat agcgccgcct
    21781 ccggcagaac ttaagcgcct gtttgcacgt ctgtagcaag aatccggcag taacaggcgt
    21841 tcgctttgcc acctggtcaa aacaggccag ccagtgccgt agctcgcatt tatccagcgg
    21901 catagcacca atgtgctgta ttacgtgatt attaaggcgc tttttcaggg cggcataatc
    21961 cacgcggttt tcctttgcgt acgactcaag ccagtaggtg agcgcatcgc caaccgttac
    22021 cggctttaac gcttcctgta cggtgtaatt aagctcatga cgtggatttt tcccctctgc
    22081 cagccatgcg cgacactggg cggctttttc cctggctgat ttcaggctca gatcaggata
    22141 atttcccagc ttaatgcgtt ccggtggtgc cccccttccc gttccggccc tgtaagtgaa
    22201 ataccaggtt aaaaggccac tggttgaatg cctgacgctc aggtttccac cgtcattaag
    22261 aaaggctgtt ttttgggcgg gtgtgccgtt gattttcctc agctgtgtat cgctcagttt
    22321 gttaagtgct ctgctcataa ttttgattcc ggtcatcaca attaactaca ccattaacta
    22381 caccgatcgt tgcacaaagg gctacaacgt gaaacaagct ggaacaagga aaatcacaaa
    22441 tctgtttata atcaaaaaca taatgaacaa tctgaaacat tatgaaacgg caaaaaacac
    22501 taattgataa aatatgtcca tacttcacgc attacgttaa gcatccgtta taatcggttg
    22561 cagataccag cctgtggatg cttaacatgg aataccaact cactcttaac tggcccgatt
    22621 ttcttgaacg tcactggcag aaacgcccgg tggtgttaaa acgcggcttt aataatttta
    22681 ttgacccgat ctctccagac gagttggcgg gtctggcgat ggaaagcgaa gttgacagtc
    22741 gactggtcag tcaccaggat ggcaaatggc aggtcagcca cggcccgttc gaaagctacg
    22801 atcatctcgg tgaaaccaac tggtcattac tggtacaggc agtaaaccac tggcatgagc
    22861 cgaccgccgc gctgatgcga ccgttccgtg aactaccgga ctggcgtatt gatgatctga
    22921 tgatttcttt ttctgtacct ggcggcggcg tcggcccgca tctcgatcag tacgacgtgt
    22981 ttatcattca gggtaccgga cgtcgtcgct ggcgagtggg cgaaaagctg caaatgaaac
    23041 agcactgccc acatccggat ctgttacagg tcgatccgtt cgaagccatc atcgatgaag
    23101 agctggagcc tggtgatatt ctttatattc cgccaggatt cccgcatgaa ggctacgcgc
    23161 tggaaaatgc gatgaactat tccgtgggtt ttcgcgcgcc aaatacgcgg gaattaatta
    23221 gcggatttgc cgattatgtg ctgcaacgtg aactgggcgg caactactac agcgatccgg
    23281 atgttccacc tcgtgctcat cctgcggatg ttctgccgca agagatggat aaactgcgtg
    23341 agatgatgct cgaattgatc aaccagccgg aacactttaa gcaatggttt ggcgagttta
    23401 tatcccagtc acgtcatgaa ctggatatcg cgccgccgga accgccttat cagccagatg
    23461 aaatctacga tgcgctgaaa caaggtgatg tgctggtgcg cctgggtggt ctgcgcgtat
    23521 tgcgcattgg cgacgacgtg tatgccaatg gtgagaagat cgattccccg caccgtccgg
    23581 cactggatgc actcgccagc aacattgcgc tgactgcgga gaattttggc gatgcgctgg
    23641 aagatccgtc attcctcgcg atgctcgcgg cgctggtcaa tagcgggtat tggttcttcg
    23701 aagggtaagt ttggatttaa gccggatgca gcatcacatg ccgcatccct tttcgcttac
    23761 ttccgttgcg ccgttaactc ggcaatacgg acgatcacct gcaccgcttt ttccatacct
    23821 tccagaatca caaactcatg cttaccatga tagttgtaac cgccagtgaa caggttcggg
    23881 caaggtaatc ccataaacga caactgcgcg ccgtcggtac cgccgcggat cggtttcagt
    23941 tccggttcaa tatcgcagtc acgcatcgcc tgctgggcga tatcgagaat atgcggatgc
    24001 tcaaccactt tctcgcgcat attgtagtaa ctgtcttcaa tcaccaattc aatgtagcaa
    24061 tcaggatgta accctttgcc cacttttttg gcgatctcca tcattttacg tttacgcgct
    24121 tcaaactgtt tacggtcgaa atcacggatg atgtagtgca tatcggcccg ttcaacggtg
    24181 cctttcatgc tcgccaggtg atagaaacct tcatagcctt ctgtcatttc cgggctttca
    24241 tccgccggaa cttccgcatg aatacgtgcc gccagcgaca gcgcatttac catcactcct
    24301 ttcgccgtgc ccggatgaac attgttaccg acaattttta tattgaccga tgcggcgttg
    24361 aagttttcaa actccagttc gcctacgcca ccaccgtcaa cagtgtaagc ccagcgggca
    24421 tcgaaggcat caacatcaaa atgtttcgcc cctttgccca cttcttcatc cggggtaaag
    24481 gcgacgcgaa tatcaccatg cggaatgttt ttctgttgca ataccgccag cgcggtcatg
    24541 atttctgcaa tacctgcttt gtcatcagca cctaacaagg ttttaccatc ggtggtaatc
    24601 aacgtctgac ccagtagctg atgcagcacc gggaacataa ccggtgataa aacttcatcg
    24661 ccgataccca gcgcaatatc gccaccgcga tagttttcaa caatttgcgg attaacattt
    24721 ttgccgctgc aatccggtga ggtatccaca tgagaaataa agccaatcgc cgggatatcg
    24781 ccagggacgt tagccggtaa cgtcgccatc aaagtgccct tctcacttaa ggtcacattg
    24841 ataagcccca tttcttcgag ctgctctttc agcagatgca ataacttcca ttggccttcc
    24901 gtgctgggaa cctgtctcac ccctgctttt gattgggtat ccagagacac gtagttcaaa
    24961 aatcgctcaa gtagtttatc catgtagtca ccctcacttt ttgtgacaac attattaaga
    25021 agcaagaaaa gacaaatatt gcgtcaggtc acttttaccc ctgcaagcgg gaatatttat
    25081 cagcattacc ttaatgaata taaagctaag ccagtaattc acaacaaaga ttggcgattc
    25141 aagcggtttg ccgtagaatt accgccctca ttaggagtca ccaaggtggt taaccacaaa
    25201 ccccgcatcg gtaagccatc cgttgcgttt acatgggaca gagtaaaaaa ttgaataaac
    25261 aaccgagttc gctttcaccg ctggtgcaat tggcgggaat tcgcaaatgc tttgatggta
    25321 aagaggtcat tccccagctg gatctgacta tcaacaatgg cgagttcctc acgctgcttg
    25381 gcccttctgg ctgcggtaaa acaaccgttc ttcgcctgat tgcaggtctg gaaactgttg
    25441 attccggacg catcatgctg gataacgagg acatcaccca cgttccggcg gaaaaccgct
    25501 atgtgaacac tgttttccaa agctatgcac ttttccccca catgaccgtg ttcgaaaatg
    25561 tggcctttgg gttgcgcatg caaaaaaccc ccgctgctga aattacgccc cgcgtgatgg
    25621 aagccctgcg gatggtgcag ctggaaacct tcgctcaacg taaaccgcat cagctctctg
    25681 gtggtcaaca gcaacgcgtc gccattgctc gagcggtggt taacaagcct cgtctgttgt
    25741 tgctggatga gtcgctctca gcgctggatt acaaactgcg taagcaaatg cagaacgagc
    25801 tgaaagcgtt acagcgtaag cttggcatta cattcgtttt cgtgactcac gaccaggaag
    25861 aagcactcac catgtcggac aggattgtgg tgatgcgcga tggtcgcatt gagcaagacg
    25921 gcacgccgcg tgaaatctac gaagagccga aaaacctgtt tgttgccggg ttcattggcg
    25981 aaattaatat gtttaacgcc acggtgatcg agcgactcga cgagcagcgc gtacgcgcca
    26041 acgttgaagg ccgcgaatgt aatatctacg ttaacttcgc cgttgaaccg gggcaaaaac
    26101 tgcatgttct gctgcgcccg gaagacttac gtgttgaaga gattaacgac gacaaccacg
    26161 ctgaagggct gattggttac gttcgtgagc gtaactacaa aggcatgacg ctggagtcgg
    26221 ttgttgaact ggaaaatggc aagatggtga tggtcagcga attcttcaat gaagacgatc
    26281 ctgactttga ccactctctc gaccaaaaaa tggccattaa ttgggtagaa agctgggagg
    26341 tcgtactggc tgatgaagaa cacaagtaag ttccagaatg tagtgattgt cactattgtc
    26401 ggttggcttg tgttgtttgt ctttctgccc aacctgatga tcattggcac cagctttttg
    26461 acccgcgacg acgccagttt cgtcaaaatg gtctttacac tggataacta cacgcgtctg
    26521 ctcgatccgc tctattttga agtgctattg cactcgctga atatggcgct gatcgccacc
    26581 ctcgcctgcc tggtgctggg ctatccattt gcctggtttc tggcgaagct gccacacaag
    26641 gtgcgtccgc tgctgctgtt tctgctgatt gttccgttct ggaccaactc attgattcgt
    26701 atctacgggc tgaaaatttt cctcagcacc aaaggctatc tcaacgagtt tttgctctgg
    26761 ctgggcgtta tcgacacacc aatccgtatc atgttcacgc ccagtgcggt gattatcggt
    26821 ctggtttaca ttctgctgcc gtttatggtg atgccgctgt actccagtat cgaaaaactg
    26881 gataagccgt tgcttgaggc ggcgcgcgat ctcggtgcca gcaagttaca gacttttatc
    26941 cgcatcatta ttccactgac gatgccgggg attattgccg gatgtctgct ggtgatgctg
    27001 ccagcgatgg gcctgttcta tgtgtccgac ctgatgggcg gtgcgaaaaa cctgctgatc
    27061 ggtaacgtca tcaaggtaca gttcctcaat attcgtgact ggccgtttgg tgcagccacc
    27121 agcattacgc tgactatcgt aatgggcctg atgttgctgg tttactggcg cgcttctcgt
    27181 ttgctgaata agaaggtgga actcgaatga tcggtcgact gcttcgcggc ggttttatga
    27241 ccgctatcta cgcgtacctg tatatcccaa tcattatttt gattgtgaac tcctttaaca
    27301 gctcgcgctt tggcatcaac tggcagggtt ttaccaccaa atggtatagc ctgctgatga
    27361 acaacgacag cctgttacag gcagcgcagc attcactaac aatggcggtg ttttcggcga
    27421 cgtttgctac gcttatcggt tcactgacgg cagttgcgct gtaccgttat cgctttcgcg
    27481 gtaagccgtt cgttagcgga atgctgtttg tggtaatgat gtcaccagat atcgtgatgg
    27541 caatttcgct gctggtgctg tttatgctgc tgggtattca gcttggcttc tggtcgctgc
    27601 tgttctcgca tatcaccttc tgcctgccat ttgtggtggt gacggtgtat tcgcgcctga
    27661 aaggttttga cgtgcggatg ctggaagcgg cgaaagatct cggtgccagc gaatttacca
    27721 ttctgcgaaa aatcattctg ccactggcaa tgccagcggt ggcggcgggc tgggtgttaa
    27781 gctttaccct gtcgatggac gatgtggtag tttcttcatt cgtcaccgga ccaagttatg
    27841 aaattctgcc gttaaaaatt tattcgatgg tcaaagtcgg cgtatcgccg gaagttaacg
    27901 cgctggcaac catattactg gtgctgtcgc tggtgatggt tattgccagc cagcttattg
    27961 ctcgtgataa aacgaaaggt aacacagggg acgttaaatg aaaaaatggt cacgccacct
    28021 gctcgcggcg ggtgctctgg cactgggcat gagcgccgct cacgccgatg acaacaacac
    28081 gctgtatttc tacaactgga ccgagtacgt gccgccagga ctgcttgaac agttcaccaa
    28141 agaaaccggt attaaggtta tctattcgac ttacgagtcg aacgaaacca tgtacgccaa
    28201 gctgaaaacg tacaaagacg gtgcctatga cctggtggtt ccttcaacct attacgtcga
    28261 taaaatgcgt aaagagggga tgatccagaa gatcgacaag tcgaagttaa cgaacttcag
    28321 caatctcgat ccagacatgc tcaacaagcc ttttgacccg aataacgact actccattcc
    28381 gtatatctgg ggtgcgacgg cgattggtgt taacggtgat gcggtggatc cgaaatctgt
    28441 caccagctgg gccgatctgt ggaagccaga gtacaaaggc agcctgctgt tgaccgacga
    28501 tgcccgtgaa gtgttccaga tggcgctgcg taagctgggc tactccggta acaccaccga
    28561 tccgaaagag attgaagctg catataacga gctgaaaaaa ctgatgccaa acgtcgcagc
    28621 gtttaactcc gataacccgg ctaacccgta catggaaggc gaagttaacc tcggcatgat
    28681 ctggaacggt tctgctttcg ttgcacgcca ggcgggtacg cctattgacg tggtgtggcc
    28741 gaaagaaggc ggcatattct ggatggacag cctggcgatc ccggcaaatg ccaaaaacaa
    28801 agaaggcgcg ctgaaattga tcaacttcct gctgcgcccg gatgtggcaa aacaggttgc
    28861 tgaaactatc ggttatccaa cgccaaacct tgcggcgcgt aagctgttaa gtccagaagt
    28921 ggcaaacgat aagacgctct acccggatgc tgaaaccatt aaaaatggcg aatggcagaa
    28981 tgacgttggc gcagccagca gcatttatga agagtattat cagaagctga aagcaggacg
    29041 ttaattcagc aagcgggcgg tggtattacc gcccgttttt tagttagcaa ggatgtggct
    29101 aatgagtcag gacagtaaag ttttatcccg ctctttcgtc ggtattgtgg tccgcttgta
    29161 ggcctgataa gcgtagcgca tcaggcaatg cttcctgctt tataatccct ccagcaactt
    29221 ttcaacaaat tctggcacca cctggcttgc cgggccgtaa tatttctcgg caaattcatt
    29281 accaacctga ctcggttcaa gattcagttc aacggtgtga gcaccatgta gtttggcttc
    29341 gtgtacaaac ccagccgccg gatagacatg cccggaagta ccaatggcaa taaagacatc
    29401 cgccatcgac aacgccatat aaatttcatc catgccgagt ggcatttcgc caaaccaaac
    29461 gacgtgcggg cgcaagggtg ccgggaactg gcaacaatgg catttatctt ctggcgtaac
    29521 gtctccggtc cagtcgagaa cctgaccact ttgcgaacaa cgaactttca gcaattcccc
    29581 atgcatatga atgacattgg tattacctgc gcgttcatgc aggttgtcta tattctgcgt
    29641 taccagcaaa aagcgatcgc cgagagcgtc ttgcagttta gccagcgcaa gatgcgcggc
    29701 gttaggctga atttctggct gctgcagctg tcgacgacgg gcgttataaa acgcttgcac
    29761 cagttcagga tcgcgatcga aaccttccgg cgttgccaca tcttcaaccc gatgttcttc
    29821 ccacaggcca tctgcggcgc gaaaggtacg aatacctgat tccgcagaaa ttcctgcccc
    29881 tgtcagtacg agtactcttg gtttttccat tgcttccggc accactttat ctctgaaaaa
    29941 aatacgctga cgcaaacgct cgcgcaggcg acgtttattt ttacgaaaac gacttaaccg
    30001 atgaccccga cgcgacagca tagcaacctc tgttgtttaa tcggttagat gtaggaaggc
    30061 cgcaccacgc attcctcccg catcaccgtg gcgcgcgcgt tcaatgcgtg gaacacgagc
    30121 tacaggtaag agatgacaag gcaacctgtc cgccagttgc gttgtgattg ccgggaaatt
    30181 cgataagcca ccaccaatga cgaccaggtc agggtcaaca atggtcagga tatttcccag
    30241 agaaaccgct aataaatcca gataacgctc aacgtgcgcc cttgcctgct catcgccttg
    30301 atcataaagc gcaataattt cgggagcctg caacggttga tgataatagt gttgatacag
    30361 ccacgcaaaa ccgcgaccag acagataatt ttcaatgcag ccatgctgac cacagccgca
    30421 gcggcgtaac gggaaatcca accccatcat ggttaacgca tcaaccggca gacgcatatg
    30481 gccaaactcg ccggtaatgt agcttttacc agtaatcggt ttgccgttga aaaccagccc
    30541 gccgccaacg ccggtgccga gaattaaccc catcaccagt ggatattgcg taaattcatc
    30601 atcccaggct tctgaaaggg caaaacagtt ggcatcgtta tcaaggcgta catcgcgatc
    30661 aagacgtgcg ctcaggtcgg cacgcagcgg tttgccgctg gcagcgggga cattagcggc
    30721 atacagctta ccatcttctg tttccggcat tcccggaata ccgatgccga cagaaccttt
    30781 acagccaaaa cgttggtcag cttcagctac cagctcacac actgcatcta aaaatgcgtc
    30841 atagctgtca cgcggtgtcg gcacccgctt ttcccactgc aactgccgac cgctatcaaa
    30901 cacgccaagc gcaatttttg ttccaccaat atcaaacccg taatacattg ccgctccttg
    30961 ttttaatgta ctgcctttac tggccgctaa ggactcgcgc agggtcaata ttactggcgc
    31021 gccgcgccgg ataccaactt gccaaaagac tcagcaacaa tgctgtgacc agtacgtaga
    31081 agacgtccag ccagtgcaat tccgatggca ggaagtcaat aaaataaata tcgctggaga
    31141 ggaactgatg accgatcagc ttttcaatcc actcaataat cggggtaagt tgcagtgaaa
    31201 caactacgcc gataaccacc ccacacaggc tgccgaacag ccctgccagc aatccatacc
    31261 agacaaagat ggcgcgaatt aaaccatctt tcgcccccag cgttcttaat actgcgatat
    31321 cgccactctt gtctttcacc gccatcacta aggtggagac gatgttgaaa caggccacgc
    31381 caatcaccag taccatcgcc agatacataa tggcgcggat catctggata tcgcgataca
    31441 tatagccgta agtaccaatc caacttttaa tataaacata gctgttggtc acttcacccg
    31501 catcgcgtac cagcttattg gcgttgaaaa catccgtcat tttaagggca atacctgaca
    31561 cgctggaacc catatcaaga tattgttggg catccgccag cgggatcatg gcaaaactgt
    31621 gatcgagttg accactcaac tgcaaaatac cggcaatgtg caaacgcaca cgttttggct
    31681 gcatcagttt atgctcggga ttcgagttgg ggatcatgat cgacacccaa tcgccctgct
    31741 tcactttcag cgcatccgcc acgcctttgc cgatgataat ttgctgttcg cccgctttaa
    31801 aattgcgcca cgcatccccc tgaacaaacg agggtaatgc gctcagacgc tgttcctgtt
    31861 gtgggttaac gcccttcacc tggattgcgc gcagattcgc tccactttcc accagaccgg
    31921 tgaaattgat atacggcgca gcggcggcaa tacccggcac tttctgcacg ttatccagtg
    31981 cttcctgcca gttagtccat ggctgattta ccgcttcgat ttcgccatgc ggcaccaccg
    32041 ccagaatgcg gttattcagt tcgcgttcaa agccgttcat cgcgcttaag ccgacgatca
    32101 ataccgccac gccgagggca atgccaatgg tagaaatgac agagattagc gacaccatgc
    32161 cgccgcgtcg ccgtccgcga ctaaaacgca ggccaatcaa taacgataaa ggcatcgcca
    32221 ttactccgcc cccatcaggc tcagttccgc cgtcagacgt ccatcacgca tttccagttg
    32281 gcggctcata cgtttcgcca gttgcaggtc gtgagtgacc accaggaaag cggtgccctg
    32341 caagcgattc aactccccaa gcaactggaa gatgctgtcg gcgttacgcg catcgaggtt
    32401 accggtaggt tcatccgcca gtaccagacg cggattattg accagcgcac gggcaatcgc
    32461 tacacgctgg cgttcgccgc cagaaagttc agatgggcgg tgattcgcac gatgctccag
    32521 ccccaccgct tttaacatct ccagtgcaca gctgttgatt tcagcgggct ttttcttgcc
    32581 aatcagcagt ggcatagcca cgttttccag ggcagtaaaa tccggcagca ggtggtgaaa
    32641 ctgataaata aagcccagct tctggttgcg cagttctgct ttcgccgctg aagacagttt
    32701 gctcatcggc tgaccgttaa agatcacatc gccggaggtt ggcgtatcca gcccgcctaa
    32761 caggtgcagc aaggtacttt taccggaacc agagctaccg acaattgcca tcatttcacc
    32821 ttcgcccacg ctgaaactga cattgagcag cacatcggtt tgcacactgc cttcctgata
    32881 gcgtttgcac aggttgtcgc attgcaacag gatcttattc ataacgtaaa gcctcagcgg
    32941 gttgtgtggc ggcagcgcgc catgaagggt aaagcgtaga cagcagcgcg atagccatcg
    33001 ccaccagcgc aataacaatg acctgtaaag gttcgatagc caccggcagc gccgcgccat
    33061 caagcaggac gccgattatc ggcatcagat tatttaactg gctggcaagc agtgcgccaa
    33121 gcgccgctcc gaggattgca ccgataatcc cggcgctagc cccttgcacc ataaagacca
    33181 tcatgatttg tcgcggagtt aagccttgcg tttgcaggat cgcgacttcg ccctgcttct
    33241 ccatcaccat cagccccagc gaggtaataa tgttaaacgc cgcaacagcg acaatcaggc
    33301 tcagcagtaa gcccatcata tttttttcca tgcgtacggc ctggaacagc tcgcctttac
    33361 ggtcacgcca gtcctgccat ttgctgcctt caggcagttt ttgctgactt aaagagtcaa
    33421 ctctcagcgg ctcatccagc cacaaacgcc agccggtaat attggctgcc ggataacgca
    33481 tcagacgcga ggcatcctca atattcacca gcatttcata gccatcgact tcactgttgg
    33541 cggcgaaagt gccaatcaca ttgaacaggc gctgacttgg aatacgcccc atcggcgtga
    33601 actggctggc agatggcacc atcacgcgga tttgatcacc gcgattgacg cccaactgtg
    33661 aggcaagttg ttcgccgagg atgacattat atttccccgg ctcgaggtca gtttgtttca
    33721 cattgaccag atacggcgtt agtggatctt tttgcgccgg gtcgataccg agcatcaccc
    33781 cgaccgccac gctgcgcgcg ctttgcagta ccacatcacc ggtagtaata ggtgcgacgc
    33841 gattaacgcc gtccagtttg actgccgttt ccgggagttg ctgcgggtta agagagccat
    33901 gctcagaaga gagaattgcc tgtggcatca ggccaaggat gttgttttgc agctcgcgct
    33961 caaagccgtt catcactgac aatactgtga ccagcgccat caccccgagg gtaatgccga
    34021 tggtagaaag ccaggagacg aaacgaccga agcgatccac tgcacgccca cgcatgtaac
    34081 gcaggccaat aaatagagcg acaggttggt acatgaaatc cgtctggttg ctgtagcaaa
    34141 gacacgagta tataagcgaa agcaagatgg gtaaacgtct gaactgtaag tgatcgtcag
    34201 ggctaatctg aaaagccccc gtaaatcaat cggctaaatt gccatatttt atcatcaatc
    34261 ttgccgcctg atcttaatct gaaaaatcat ccaatacaga cagataccca ttacacctcg
    34321 ggtcataacc agcaggatca accgcatgta aattaaaaaa gagcgtgttg ttctgatgaa
    34381 acaaaaagag ctatggatta accagatcaa agggttatgt atttgtctgg tggtgattta
    34441 tcactcggtc attacctttt atccgcatat gaccactttt cagcatccgt tatcggaagt
    34501 cctgagcaaa tgctggatct atttcaatct ttaccttgcc ccctttcgta tgccggtttt
    34561 tttctttata tccggctatt tgattcgccg ctatatcgac agcgtaccgt ggggaaattg
    34621 tctcgataaa cggatctgga acatcttctg ggtgctggcg ctttggggcg tggtgcagtg
    34681 gctggcgtta agtgcgctga accagtggct ggcacctgag cgcgatttaa gcaatgcctc
    34741 caatgccgct tatgccgatt ccaccggtga gttcctgcac gggatgatca ccgccagcac
    34801 cagcttgtgg tatctgtatg cgttaattgt ctatttcgtg gtatgtaaaa tttttagccg
    34861 tctggcgctg ccactattcg ccttgtttgt actgctgagt gtggcggtta atttcgttcc
    34921 cacgccgtgg tggggaatga acagtgtgat acgcaatttg ccttattaca gccttggcgc
    34981 atggtttggc gcaacaataa tgacctgtgt taaagaggtg ccgttgcgcc gccatctgct
    35041 gatggcttct ttgcttaccg ttctggcggt cggtgcctgg ttgtttacta tctcgctgct
    35101 gttgtcgctg gtatcgattg tggtgatcat gaagctgttt tatcagtacg agcaacgatt
    35161 cggtatgcgc tccaccagcc tgctgaatgt gattggctcc aacaccattg ctatctacac
    35221 cacccatcgc attctggttg aaatattcag cttaactctg cttgcgcaaa tgaacgcagc
    35281 acgctggtcg ccgcaagtcg aactgacact cctgctggtt tatccctttg tcagtttgtt
    35341 catctgtact gttgcgggct tgctggtaag aaaactttca cagcgtgcat tcagcgatct
    35401 gttgttctcc ccgccttcac tgcccgctgc cgtcagttac tctcgttaaa ccatcacccc
    35461 agccggggcg attcaatttg ctaaaccatg tcgattacgg ataatgaaca gcattgctta
    35521 tcagagatat gcccccatat gttgaggcat atcctaacga gaatctgaca accgttatgc
    35581 ctgaacaata tcgttatacg ctgcccgtca aagcgggtga gcagcgtctg ctgggcgagt
    35641 taaccggcgc agcctgtgca acgctggtag cggaaattgc cgaacgtcac gccggtccgg
    35701 tggtactcat tgcaccagat atgcaaaatg ctctgcgttt gcatgatgaa atcagccagt
    35761 tcaccgatca gatggtgatg aatctggcgg actgggaaac tcttccctac gacagttttt
    35821 cgcctcatca ggacattatc tcctcgcgcc tttccaccct ttaccagcta ccgacgatgc
    35881 agcgtggcat actgattgtt ccggtgaata cgcttatgca gcgcgtttgc ccacacagtt
    35941 ttctccacgg tcatgcgctg gtgatgaaaa aaggtcagcg cctgtcacga gatgcattac
    36001 gaacccaact ggacagcgcc ggttatcgcc atgttgacca ggtgatggag cacggcgaat
    36061 acgccacgcg cggcgcgttg ctggatctct tcccgatggg gagtgagctg ccttatcgtc
    36121 ttgatttctt tgatgatgaa atcgacagcc tgcgggtgtt tgacgtcgac agccagcgca
    36181 cgctggagga agtagaagcg atcaatctgc tgcccgcgca cgaatttccg accgataaag
    36241 cggcaattga actgttccgc agccagtggc gcgatacctt cgaagtgaag cgcgatccgg
    36301 aacatattta ccagcaagtg agtaaaggca cattacctgc tgggatcgag tactggcagc
    36361 cattgttctt cagcgaacca ctgccaccgc tgttcagtta tttccctgcc aataccttgc
    36421 tcgtgaatac tggcgatctg gaaaccagtg ccgaacgttt ccaggctgac acgctggcgc
    36481 gttttgagaa tcgcggcgtc gatccgatgc gcccgctgtt gccaccacaa tcgctctggc
    36541 tgcgggtgga cgagctcttc tcagagctga aaaactggcc ccgagtgcag ctaaaaactg
    36601 aacatttacc gacaaaagcc gcgaatgcca atttaggttt ccagaaactg ccagacctgg
    36661 ccgttcaggc gcaacaaaaa gcgccgctgg atgcgctgcg taagttcctc gagactttcg
    36721 acggtccggt ggtgttctcg gtagaaagtg aaggtcgccg tgaagcgctg ggtgaactgc
    36781 tcgcacgaat taaaattgct ccgcaacgca ttatgcgtct tgatgaagcc agcgaccgtg
    36841 ggcgttatct gatgattggc gctgccgaac atggttttgt cgataaggcg cgtaatctgg
    36901 cgctgatctg cgaaagcgat ctgctcggtg aacgtgttgc ccgccgtcgt caggattctc
    36961 gccgcaccat caaccccgat acactgatcc gtaaccttgc ggagctgcat attggtcagc
    37021 cggtggtcca tctggagcac ggcgtcggtc gctatgccgg aatgaccacg ctggaagctg
    37081 gcggcattac tggcgagtac ctgatgctca cctatgccaa cgacgccaaa ctgtatgttc
    37141 cggtgtcgtc actgcatctg attagccgtt acgcaggtgg cgcggaagaa aacgccccgc
    37201 tgcataaact tggcggcgat gcgtggtcac gcgcgcggca gaaagcggcg gaaaaagtgc
    37261 gtgatgtggc ggcggaattg ctggatatct acgcgcaacg cgccgccaaa gagggcttcg
    37321 cgtttaaaca cgatcgtgag cagtatcagt tattctgcga cagcttcccg tttgaaacca
    37381 cgccggatca ggcgcaggcc attaatgcgg tacttagcga catgtgtcag ccgctggcaa
    37441 tggatcgtct ggtgtgcggc gatgttggct ttggtaaaac agaagtggcg atgcgcgccg
    37501 ctttcctggc agtagataac cacaagcagg tagcggtgtt ggtgcctacc acccttcttg
    37561 cgcagcagca ttacgacaac ttccgcgacc gtttcgccaa ctggccggta cgtatcgaaa
    37621 tgatctcccg tttccgcagc gccaaagagc agacgcaaat ccttgcggaa gtggcggaag
    37681 ggaaaatcga tattctgatc ggtacgcaca aactgctgca aagtgacgtc aagtttaaag
    37741 atttaggcct gctgattgtc gatgaagaac accgcttcgg ggtgcgtcat aaagagcgca
    37801 ttaaagcgat gcgcgcgaac gtggatattc tgacgcttac tgcaacgccg atcccacgta
    37861 cgctgaatat ggcaatgagc ggaatgcgtg acctgtcgat tatcgccacg ccgcccgccc
    37921 gtcgtctggc agttaaaacc tttgtccgtg agtatgacag cctggtggtc cgggaggcga
    37981 tcctgcgtga aattttgcgc ggaggacagg tttattatct ctacaatgat gtggaaaaca
    38041 ttcagaaagc cgccgaacgg ctggcagaac tggtgcctga agcacggatt gccatcggtc
    38101 acgggcagat gcgcgagcgc gaactggaac gggtgatgaa tgatttccat catcaacgtt
    38161 tcaacgtgct ggtttgtacc accattatcg aaaccgggat cgacatcccg acagccaaca
    38221 ccattatcat tgaacgtgcg gatcacttcg gtctggcgca gctgcaccag ttacgcggtc
    38281 gcgtcggacg ttcacatcat caggcatatg catggctgct gacgccgcat ccaaaagcga
    38341 tgactaccga tgcacaaaaa cgtcttgaag cgattgcctc gctggaagat ctcggggcag
    38401 gttttgcgct ggcaacgcac gatctggaga ttcgcggcgc gggtgaactg cttggcgaag
    38461 aacaaagcgg ctcaatggaa accatcggtt tctcgctgta tatggagttg ctggaaaacg
    38521 ccgtcgatgc actgaaagcc ggacgcgagc cgtcgctgga agatctcacc agccagcaaa
    38581 cagaagtcga gctgcggatg ccgtcgctat tgccagatga tttcatccct gacgtgaaca
    38641 cgcgtctgtc gttctacaaa cgtattgcca gcgccaaaac ggaaaacgaa ctggaagaga
    38701 tcaaagtcga gcttatcgat cgcttcggcc tgctgccaga tccggcgcgt accctgctgg
    38761 atattgcccg tctgcgccag caagcgcaga aactggggat caggaagctg gaaggtaatg
    38821 agaaaggcgg ggtgatcgaa tttgccgaga agaatcacgt taatccggcc tggttgattg
    38881 gtttgctgca aaaacagccg cagcattacc gccttgatgg tccgacgcgc ctgaaattta
    38941 ttcaggattt gagtgagcgg aaaacgcgta tcgaatgggt acgccagttt atgcgtgaac
    39001 tggaagagaa cgcgatcgct taatgtaaat cgggggcgtt tctgcgcccc catctgcaac
    39061 atttacaaat tctttgcact ttcctgcact atccgacact gtcaccatcc ataattcagg
    39121 cttatctgtt tattacaata accttatatt tattatgtat ttttggtgat gatcaaaacg
    39181 cgtttttctc gctggctaac gttttttacg ttcgccgctg ccgtggcgct ggcgctaccg
    39241 gcaaaagcca acacctggcc gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa
    39301 tttcatgtgg tggaaaatga cggtggttct ctggaagcca tcgccaaaaa atataacgtc
    39361 ggctttctcg ctctgttaca agctaacccc ggcgttgatc cttacgtacc gcgcgcgggc
    39421 agcgtgttaa cgatcccgtt gcaaacccta cttccagacg cgccgcgcga aggcattgtg
    39481 atcaacattg cggagctgcg tctctattac tacccgccgg gtaaaaattc ggtaaccgtg
    39541 tatccaatag gtattggtca gttaggtggt gacacgctga caccgacaat ggtgaccacc
    39601 gtttcagaca aacgtgcaaa cccaacctgg acgccaacgg caaacatccg cgcccgttat
    39661 aaagcacagg gaattgagtt gcctgcggta gtgccggctg gaccggataa cccaatgggc
    39721 catcatgcga ttcgtctggc ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc
    39781 gatttcggca ttggcatgcg ggtaagttct ggctgtattc gtctgcggga tgacgatatc
    39841 aaaacactct ttagccaggt caccccaggc accaaagtga atatcatcaa cactccgata
    39901 aaagtctctg ccgaaccaaa cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag
    39961 aagattgatg acgatccgca gctgctgcca attacgctga atagcgcaat gcaatcattt
    40021 aaagatgcag cacaaactga cgctgaagtg atgcaacatg tgatggatgt ccgttccggg
    40081 atgccggtgg atgtccgccg tcatcaagtg agcccacaaa ctctgtaaat cctggataaa
    40141 aaaaatcccc gcgacacggc atgtcgcggg gaaaaaatca gcagtagcag gcattaatga
    40201 gggttaatgc ttatttataa attactgctg ttccatggag ggtattcgga ccggttacag
    40261 aagtaatacg gaaagatttt gcgcccatct catccgcttt ttgcgccagc tgctcttcca
    40321 gcgatcccag atttgtcccc gcgttagcac tgatagtacc gactttttgt tggccttctg
    40381 gcgttgactg aacttcgaca gccgcaaagt tggcaaatga catggagctt aaaatcgccg
    40441 cagcgatgag gtttttacgt ttttcataat agtggcctta tgcagatg
//
LOCUS       DAZSNM010000013        37318 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZSNM010000013 DAZSNM010000000
VERSION     DAZSNM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37318
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.013"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..2166)
                     /gene="glnD"
                     /locus_tag="ACHW42_000612"
     CDS             complement(<1..2166)
                     /gene="glnD"
                     /locus_tag="ACHW42_000612"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR8942567.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFA"
     gene            complement(2228..3022)
                     /gene="map"
                     /locus_tag="ACHW42_000613"
     CDS             complement(2228..3022)
                     /gene="map"
                     /locus_tag="ACHW42_000613"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR8942568.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            3390..4115
                     /gene="rpsB"
                     /locus_tag="ACHW42_000614"
     CDS             3390..4115
                     /gene="rpsB"
                     /locus_tag="ACHW42_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR8942569.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            4373..5224
                     /gene="tsf"
                     /locus_tag="ACHW42_000615"
     CDS             4373..5224
                     /gene="tsf"
                     /locus_tag="ACHW42_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR8942570.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            5371..6096
                     /gene="pyrH"
                     /locus_tag="ACHW42_000616"
     CDS             5371..6096
                     /gene="pyrH"
                     /locus_tag="ACHW42_000616"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR8942571.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            6388..6945
                     /gene="frr"
                     /locus_tag="ACHW42_000617"
     CDS             6388..6945
                     /gene="frr"
                     /locus_tag="ACHW42_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR8942572.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            7037..8233
                     /gene="ispC"
                     /locus_tag="ACHW42_000618"
     CDS             7037..8233
                     /gene="ispC"
                     /locus_tag="ACHW42_000618"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR8942573.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            8422..9180
                     /gene="ispU"
                     /locus_tag="ACHW42_000619"
                     /gene_synonym="uppS"
     CDS             8422..9180
                     /gene="ispU"
                     /locus_tag="ACHW42_000619"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR8942574.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            9193..10050
                     /gene="cdsA"
                     /locus_tag="ACHW42_000620"
     CDS             9193..10050
                     /gene="cdsA"
                     /locus_tag="ACHW42_000620"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR8942575.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            10062..11414
                     /gene="rseP"
                     /locus_tag="ACHW42_000621"
     CDS             10062..11414
                     /gene="rseP"
                     /locus_tag="ACHW42_000621"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR8942576.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            11444..13876
                     /gene="bamA"
                     /locus_tag="ACHW42_000622"
     CDS             11444..13876
                     /gene="bamA"
                     /locus_tag="ACHW42_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR8942577.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            13998..14483
                     /gene="skp"
                     /locus_tag="ACHW42_000623"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             13998..14483
                     /gene="skp"
                     /locus_tag="ACHW42_000623"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR8942578.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            14487..15512
                     /gene="lpxD"
                     /locus_tag="ACHW42_000624"
     CDS             14487..15512
                     /gene="lpxD"
                     /locus_tag="ACHW42_000624"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR8942579.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            15617..16072
                     /gene="fabZ"
                     /locus_tag="ACHW42_000625"
     CDS             15617..16072
                     /gene="fabZ"
                     /locus_tag="ACHW42_000625"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR8942580.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            16076..16864
                     /gene="lpxA"
                     /locus_tag="ACHW42_000626"
     CDS             16076..16864
                     /gene="lpxA"
                     /locus_tag="ACHW42_000626"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR8942581.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            16864..18012
                     /gene="lpxB"
                     /locus_tag="ACHW42_000627"
     CDS             16864..18012
                     /gene="lpxB"
                     /locus_tag="ACHW42_000627"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR8942582.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            18009..18605
                     /gene="rnhB"
                     /locus_tag="ACHW42_000628"
     CDS             18009..18605
                     /gene="rnhB"
                     /locus_tag="ACHW42_000628"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR8942583.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            18642..22124
                     /gene="dnaE"
                     /locus_tag="ACHW42_000629"
     CDS             18642..22124
                     /gene="dnaE"
                     /locus_tag="ACHW42_000629"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR8942584.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            22137..23096
                     /gene="accA"
                     /locus_tag="ACHW42_000630"
     CDS             22137..23096
                     /gene="accA"
                     /locus_tag="ACHW42_000630"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR8942585.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            23195..25336
                     /gene="ldcC"
                     /locus_tag="ACHW42_000631"
     CDS             23195..25336
                     /gene="ldcC"
                     /locus_tag="ACHW42_000631"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR8942586.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            25393..25782
                     /locus_tag="ACHW42_000632"
     CDS             25393..25782
                     /locus_tag="ACHW42_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR8942587.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            25847..27145
                     /gene="tilS"
                     /locus_tag="ACHW42_000633"
     CDS             25847..27145
                     /gene="tilS"
                     /locus_tag="ACHW42_000633"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR8942588.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(27194..27454)
                     /gene="rof"
                     /locus_tag="ACHW42_000634"
     CDS             complement(27194..27454)
                     /gene="rof"
                     /locus_tag="ACHW42_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR8942589.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(27441..27641)
                     /locus_tag="ACHW42_000635"
     CDS             complement(27441..27641)
                     /locus_tag="ACHW42_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR8942590.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            27807..28352
                     /locus_tag="ACHW42_000636"
     CDS             27807..28352
                     /locus_tag="ACHW42_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR8942591.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            28349..28771
                     /gene="arfB"
                     /locus_tag="ACHW42_000637"
     CDS             28349..28771
                     /gene="arfB"
                     /locus_tag="ACHW42_000637"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFR8942592.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSRELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            28785..29495
                     /gene="nlpE"
                     /locus_tag="ACHW42_000638"
                     /gene_synonym="cutF"
     CDS             28785..29495
                     /gene="nlpE"
                     /locus_tag="ACHW42_000638"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR8942593.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(29650..30474)
                     /locus_tag="ACHW42_000639"
     CDS             complement(29650..30474)
                     /locus_tag="ACHW42_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR8942594.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(30527..32245)
                     /gene="proS"
                     /locus_tag="ACHW42_000640"
     CDS             complement(30527..32245)
                     /gene="proS"
                     /locus_tag="ACHW42_000640"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR8942595.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(32356..33063)
                     /gene="tsaA"
                     /locus_tag="ACHW42_000641"
     CDS             complement(32356..33063)
                     /gene="tsaA"
                     /locus_tag="ACHW42_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR8942596.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(33060..33464)
                     /gene="rcsF"
                     /locus_tag="ACHW42_000642"
     CDS             complement(33060..33464)
                     /gene="rcsF"
                     /locus_tag="ACHW42_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR8942597.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(33582..34397)
                     /gene="metQ"
                     /locus_tag="ACHW42_000643"
     CDS             complement(33582..34397)
                     /gene="metQ"
                     /locus_tag="ACHW42_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR8942598.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(34437..35090)
                     /locus_tag="ACHW42_000644"
     CDS             complement(34437..35090)
                     /locus_tag="ACHW42_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR8942599.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(35083..36114)
                     /gene="metN"
                     /locus_tag="ACHW42_000645"
     CDS             complement(35083..36114)
                     /gene="metN"
                     /locus_tag="ACHW42_000645"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR8942600.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            36302..36877
                     /gene="gmhB"
                     /locus_tag="ACHW42_000646"
     CDS             36302..36877
                     /gene="gmhB"
                     /locus_tag="ACHW42_000646"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR8942601.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            37241..>37318
                     /locus_tag="ACHW42_000647"
     rRNA            37241..>37318
                     /locus_tag="ACHW42_000647"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         9066 a         9102 c        10195 g         8955 t
ORIGIN      
        1 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
       61 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
      121 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
      181 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
      241 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
      301 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagtgtttc
      361 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagcgcagac gattttccgt
      421 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
      481 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
      541 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
      601 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
      661 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
      721 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
      781 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
      841 gctatggcga tgcattggca atagtccgcg ccgcaccgct ccggggtgac gcagaatgct
      901 caaaaacagt tttcgtgctt gcggaatatt acacagcggt tgttgcagat ggcgacgggc
      961 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     1021 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     1081 gtcgattagc gtaccgcgta gctgaaactc atcgtcgagt ggacgtggtt tttcgtcggc
     1141 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     1201 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     1261 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacagac gattatcgta
     1321 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     1381 taattccgcc cgctccgctg aggttaagaa gccaaacccg accatttcat ccagcgatgt
     1441 tgcgccaaaa tgacggcggg ccacccattg cagagtgtga atatcgcgca agccgccagg
     1501 gctacttttg atgtctggtt caaggttgta gctggtgcca tggtaacgct gatggcgctg
     1561 gttctgttct tcaactttcg ctgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     1621 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     1681 taaattggtg gcgacggtta aatccgataa cccttccaac atgcactctt caagcgtgcg
     1741 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     1801 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     1861 tggatgcagc tcgccacgac cgtagccacc gacggcaacc aatgccaggt cggcaatctg
     1921 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tctatgaact cggtacgtgc
     1981 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     2041 agtatcgata tgggctttta tcccacagac ggttaattca tcacggggcc aggcgcatgg
     2101 attttgcggt tgaccgggca gggtggagag agcggtgttt gcgtactgtt ctggaagggt
     2161 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     2221 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     2281 aaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     2341 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     2401 tgaccattgg ctcaatggtg aacgtcatcc ccggtttcag cacgacgttg gtttcacggg
     2461 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     2521 attcacgaac gacggagaag ccttctgctt cgacaaattt ctgaatcgcc gcaccgattt
     2581 cgcgcagatt aatgcctggt tttaccatgc gtagcgccag gtacaggctt tcttgcgtga
     2641 tgcggcacag gcgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     2701 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     2761 gcagcttagc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     2821 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     2881 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     2941 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     3001 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     3061 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     3121 caaacgtgcc actgaaggtt ttctataata gaaaattcga cgtctgatgc tgtacacagc
     3181 gccaacaatt attggtgtcc acgacgtatt tgtggtataa agcgcgccgg acttccgatc
     3241 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     3301 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     3361 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     3421 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     3481 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     3541 acgaagctct ggctgaactg aacaagattg tttctcgcaa aggtaaaatc cttttcgttg
     3601 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
     3661 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
     3721 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgac aagctgacca
     3781 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
     3841 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
     3901 ttgctatcaa agaagcaaac aacttgggta ttccggtatt tgctatcgtt gataccaact
     3961 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
     4021 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
     4081 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
     4141 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
     4201 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
     4261 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
     4321 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
     4381 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
     4441 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
     4501 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
     4561 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
     4621 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
     4681 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
     4741 agcgaaaatt ggtgaaaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
     4801 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
     4861 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
     4921 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
     4981 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
     5041 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
     5101 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgtttcg aagtgggtga
     5161 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
     5221 ttaattatca aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
     5281 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
     5341 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
     5401 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
     5461 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
     5521 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
     5581 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcttggca
     5641 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
     5701 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
     5761 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
     5821 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
     5881 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
     5941 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
     6001 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
     6061 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
     6121 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
     6181 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
     6241 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
     6301 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
     6361 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
     6421 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
     6481 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
     6541 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
     6601 cgttcaatgt ctccggccgt tgaaaaagcg attatggcct ccgatctcgg cctgaacccg
     6661 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
     6721 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaatgtg
     6781 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
     6841 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
     6901 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
     6961 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
     7021 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
     7081 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
     7141 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
     7201 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac aggggagccg
     7261 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggaggttga
     7321 tcaggtgatg gcagccattg ttggtgctgc tgggctgtta cctacgcttg ctgcgatccg
     7381 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
     7441 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
     7501 tatttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
     7561 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
     7621 gcgcgatttg gcaacaatga cgcccgatca agcttgccgt catccgaact ggtcgatggg
     7681 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
     7741 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
     7801 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
     7861 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
     7921 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgagcgtta
     7981 tccatgcctg aaactggcga tggaggcgtt cgaacaaggc caggcagcga cgacagcatt
     8041 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
     8101 tatcgctgcg ttgaatttat ccgtactgga aaaaatggat atgcgcgaac cacaatgtgt
     8161 ggacgatgtg ttatctgttg atgcgaacgc gagtgaagtc gccagaaaag aggtgatgcg
     8221 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
     8281 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
     8341 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
     8401 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
     8461 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
     8521 caagggaaga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
     8581 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
     8641 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
     8701 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
     8761 cgttttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agccggaaat
     8821 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
     8881 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
     8941 gagatgttaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
     9001 actgggggag agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
     9061 tactttacag atgttctctg gcccgatttc gatgaacaag actttgaagg ggcgttaaat
     9121 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
     9181 tgggggtcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
     9241 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca ttgtaacgct ggtggtctgc
     9301 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
     9361 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
     9421 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
     9481 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
     9541 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
     9601 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
     9661 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
     9721 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
     9781 ctcgctactg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
     9841 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
     9901 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
     9961 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    10021 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    10081 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    10141 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    10201 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatcgccctg atcccgttgg
    10261 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    10321 atgccttcaa taataaatct gtcggccaac gagcggcgat tattgccgca ggtccggttg
    10381 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    10441 tacgtccggt ggttggcgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    10501 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    10561 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttgaca
    10621 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    10681 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    10741 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    10801 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    10861 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    10921 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    10981 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    11041 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    11101 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    11161 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    11221 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    11281 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    11341 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    11401 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    11461 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    11521 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggccc tcctcagtat
    11581 gccggtgcgt acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    11641 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    11701 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    11761 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    11821 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    11881 atatagcgcc agcgttaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    11941 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    12001 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    12061 gaatgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgatc ttgaaaccct
    12121 gcgcagctac tatctggacc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    12181 tctgacgcca gataaaaaag gtatttacgt cacggtgaac atcaccgaag gcgatcagta
    12241 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    12301 actgactaag atcgagccgg gcgagctgta taacggcact aaagtgacca aaatggaaga
    12361 tgacattaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    12421 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    12481 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    12541 tcgcgaaatg cgtcagatgg aaggtgcatg gttggggagc gatctggtcg atcagggtaa
    12601 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    12661 gggtagcccg gaccaggttg atgtagtcta caaggtgaaa gaacgtaaca ctggtagctt
    12721 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    12781 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    12841 gacctatgct gaactgtcgg taaccaaccc gtacttcacc gtagatggcg taagcctcgg
    12901 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    12961 caagagttat ggtacagacg tgacgttggg cttcccgatt aacgaatata actcgctgcg
    13021 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    13081 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    13141 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    13201 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    13261 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    13321 atgggttgtt ctgggtcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    13381 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    13441 taccattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    13501 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    13561 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    13621 cgataagtat gctaactcgg ttcgtacttc attcttctgg gatatgggta ccgtttggga
    13681 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    13741 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    13801 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    13861 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    13921 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    13981 atggtaagga gtttattgtg aaaaagtggt tattagctgc aggtcttggt ttagcactgg
    14041 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    14101 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    14161 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaaactg cagtccatga
    14221 aagcgggcag cgatcgcacc aaactggaaa aagacgtgat ggctcagcgc cagacttttg
    14281 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    14341 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    14401 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    14461 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    14521 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    14581 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    14641 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    14701 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    14761 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    14821 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    14881 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    14941 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    15001 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    15061 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    15121 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    15181 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    15241 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    15301 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    15361 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    15421 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    15481 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    15541 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    15601 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    15661 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    15721 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    15781 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    15841 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    15901 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    15961 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    16021 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    16081 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    16141 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    16201 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    16261 ttatcagttt gcctccatcg gcgaagttaa ccaggatttg aaatatgctg gcgaaccgac
    16321 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    16381 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    16441 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    16501 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    16561 ctgcatcatt ggtgcgcatg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    16621 tccttatgtt attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    16681 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctaat
    16741 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    16801 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    16861 ttaatgactg accagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    16921 atcctggggg ccggtttaat ccgcgctctg aaagaacgtg tgcccaacgc ccgctttgtt
    16981 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cctggtacga aatggaagaa
    17041 ctggcggtga tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    17101 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    17161 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    17221 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    17281 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    17341 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    17401 ggtgcccgtg atgtgctggg gatcccttac gatgcccact gtctggcatt gttgccgggc
    17461 agccgtggtg cagaagtcga aatgcttagt gccgatttct tgaaaactgc ccagcttttg
    17521 cgccagacat atccggatct cgaaatcgtg gtgccgctgg tgaatgccaa acgccgcgag
    17581 cagtttgaac gcatcaaagc tgaagtcgcg ccagacctgt cggttcattt gctggatgga
    17641 atgggccgtg aggcgatggt cgccagcgat gcggcactac tggcgtcggg gacggcagcc
    17701 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    17761 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    17821 ggcagagagt tagtcaaaga gttattgcag gaagagtgtg agccgcaaaa actggctgcg
    17881 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    17941 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    18001 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    18061 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    18121 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    18181 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    18241 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    18301 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    18361 ctgcgatggc tgtggtgaaa ggggatagcc gcgtaccaga aatcagtgcc gcgtctatcc
    18421 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    18481 gttttgccca acataaaggg tatccaaccg cttttcatct ggaaaaactg gctgaatatg
    18541 gcgcgactga acatcatcgt cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    18601 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    18661 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cttggccaaa accgcaccgc
    18721 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    18781 tttgcggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    18841 gggcagattt taatgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    18901 tggcggcgaa caataccggc tatcagaatc tggcgttgct gatctcaaaa gcgtatcagc
    18961 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    19021 ggttgatcct tctttccggc gggcgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    19081 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    19141 ttctcgagct gatccgcacc ggcaggccgg atgaagaaag ctatctgcac gcggcggtgg
    19201 aactggcgga agcgcgcggt ttgcccgtcg tggcgactaa cgatgtgcgc tttatcgaca
    19261 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    19321 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    19381 gtgagctgtt tgccgacatc ccagaagccc ttgccaacac cgttgagatc gccaaacgct
    19441 gtaacgtaac tgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    19501 gcaccgaaga ttatctggtc aagcgtgcaa aagaaggcct ggaagagcgt ctggcatttt
    19561 tattccctga cgaggaagaa cgtcttaaac gccgcccgga atatgacgaa cgtctggaga
    19621 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    19681 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    19741 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    19801 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    19861 gtatggagaa acgcgatcag gttatcgaac acgtggcgga catgtacggt cgtgatgcgg
    19921 tatcgcagat tatcaccttc ggtacgatgg cggcgaaagc ggtgatccgc gacgtaggcc
    19981 gcgtgctggg gcatccgtac ggctttgtcg accgtatctc gaaactgatc ccgcccgatc
    20041 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    20101 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    20161 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    20221 cgctttattg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    20281 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    20341 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccaccg ctggatatcg
    20401 ccgcgatccc gctggatgac aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    20461 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    20521 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc tggtccgttg cagtcaggga
    20581 tggtggataa ctttatcgac cgtaaacatg gtcgtgaaga gatctcctat ccggacgtgc
    20641 agtggcagca tgaaagcctg aaatcggtac tggagccaac ctacggcatt atcttgtatc
    20701 aggaacaggt catgcagatt gcccaggtgc tttctggtta taccctcggt ggcgcggata
    20761 tgctgcgccg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    20821 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    20881 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    20941 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    21001 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    21061 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    21121 acgatggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    21181 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    21241 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    21301 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    21361 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    21421 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    21481 aggtggtatt agatggggaa cgtgaaacgt taggcctgta cctgaccgga caccctatca
    21541 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    21601 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    21661 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    21721 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    21781 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    21841 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    21901 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaac
    21961 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    22021 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    22081 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    22141 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    22201 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    22261 gtctgcgtga aaaaagcgta gaactgacgc gtaaaatctt cgccgatctc ggtgcatggc
    22321 agattgcgca actggcacgc catccgcagc gtccttatac cctggattac gttcgcctgg
    22381 catttgatga atttgacgaa ctggctggcg accgtgcgta tgcagacgat aaagctatcg
    22441 tcggtggtat cgcccgtctc gatggtcgtc cggtgatgat cattggtcat caaaaaggtc
    22501 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    22561 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    22621 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    22681 cacgcaacct gcgtgaaatg tctcgccttg gcgtaccggt agtttgtacg gttatcggtg
    22741 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    22801 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    22861 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    22921 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    22981 tggcggcatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    23041 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    23101 caaaagttct gaaaaagggt cacttcggtg gccctttttt atcgccacgg tttgagcagg
    23161 ctatgattaa ggaaggattt tccaggagga acacatgaac atcattgcca ttatgggacc
    23221 gcatggcgtc ttttataaag atgagcccat caaagaactg gagtcggcgc tggtggcgca
    23281 aggctttcag attatctggc cacaaaacag cgttgatttg ctgaaattta tcgaacataa
    23341 ccctcgaatt tgcggcgtga tttttgactg ggatgagtac agtctcgatt tatgcagcga
    23401 tatcaatcaa cttaatgaat atctcccgct ttatgccttc atcaacaccc actcgacgat
    23461 ggatgtcagc gtgcaggata tgcggatggc gctctggttt tttgaatatg cgctggggca
    23521 ggcggaagat atcgccattc gtatgcgtca gtacaccaac gaatatcttg ataacattac
    23581 gccgccgttc acgaaagcct tgtttaccta cgtcaaagag cggaagtaca ccttttgtac
    23641 gccggggcat atgggcggca ccgcatatca aaaaagcccg gttggctgtc tgttttatga
    23701 ttttttcggc gggaataccc ttaaggctga tgtctctatt tcggtcaccg agcttggttc
    23761 gttgcttgac cacaccgggc cacacctgga agcggaagag tacatcgcgc ggacttttgg
    23821 cgcggaacag agttatatcg ttaccaacgg aatatcgacg tcgaacaaaa ttgtgggtat
    23881 gtacgccgcg ccatccggca gtacgctgtt gatcgaccgc aactgccata aatcactggc
    23941 gcatctgttg atgatgaacg atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt
    24001 ggggattctg ggggggatcc cgcgccgtga atttactcgc gacagcatcg aagagaaagt
    24061 cgctgccact acgcaagcac aatggccggt tcatgcggtg atcaccaact ccacctatga
    24121 tggtttgctc tacaacaccg actggatcaa acagacgctg gatgtcccgt cgatccactt
    24181 cgattccgcc tgggtgccgt atactcattt tcatccaatc taccagggta aaagtggtat
    24241 gagcggcgag cgtgttgcgg gaaaagtttt cttcgaaacg caatcgaccc acaaaatgct
    24301 ggcggcgtta tcgcaggctt cactgatcca cattaaaggc gagtatgacg aagaggcgtt
    24361 taacgaagcc tttatgatgc ataccaccac ctcgcccagt tatcccattg ttgcttcggt
    24421 taagacggcg gcggcgatgc tgcgtggtaa tccgggcaaa cggctgatta accgttcggt
    24481 ggagcgggcg ctgcattttc gcaaagaggt ccagcggctg cgggaagagt ctgacggttg
    24541 gtttttcgat atctggcaac cgccgcaggt ggatgaagcc gaatgcaggc ccgttgcgcc
    24601 tggcgaacaa tggcacggct ttaacgatgc ggatgccgat catatgtttc tcgatccggt
    24661 taaagtcact attttgacac cggggatgga cgaacagggc aatatgagtg aggaggggat
    24721 cccggcggcg ctggtagcaa aattcctcga cgaacgtggg atcgtagtag agaaaaccgg
    24781 cccttataac ctgctgtttc tctttagtat tggcatcgat aaaaccaaag caatgggatt
    24841 attgcgtggg ttgacggaat ttaagcgctc ttacgatctc aacctgcgga tcaaaaatat
    24901 gctgcccgat ctctatgctg aagatcccga tttctaccgc aatatgcgta ttcaggatct
    24961 ggcgcaaggg atccataagc tgattcgtaa acacgatctt tccggtttga tgttgcgggc
    25021 attcgatact ttgccggaaa tgatcatgac gccacatcag gcatggcaga gacagattaa
    25081 aggtgaagta gaaaccattg cgctggaaca actggtcggt agagtatcgg caaatatgat
    25141 cctgccttat ccaccgggcg taccgctgtt gatgccagga gaaatgctga ccaaagagag
    25201 ccgcacggta ctcgattttc tactgatgct ttgttccgta gggcaacatt accccggttt
    25261 tgaaacggat attcacggcg cgaaacagga cgaagacggc gtttaccgcg tacgagtcct
    25321 aaaaatggcg ggataacttg ccagagcggc ttccgggcga gtaacgtgct gttaacaaat
    25381 aaaggagacg ttatgctggg tttaaaacag gttcaccata ttgcgattat tgcgacggat
    25441 tatgcggtga gcaaagcttt ctactgcgat attcttggtt tcacgctgca aagcgaagtc
    25501 tatcgcgaag cgcgcgactc gtggaaaggg gatttggcgc ttaatgggca atatgtgatt
    25561 gagcttttct cttttccgtt cccgccggag cgccccagcc gaccggaagc ttgcggcctg
    25621 cgtcatctgg cttttagcgt tgatgacatc gatgcggcag tggcgcacct tgaaagccat
    25681 aacgtgaagt gtgaagccat ccgtgtcgat ccatacacgc aaaaacgctt caccttcttt
    25741 aacgatccgg acgggctgcc gttggaactg tatgagcagt aaggcttgtc atcgccgcat
    25801 ttgcccggta acgtgccggg cattgctact gtaaaatcgc accatcatga cactcacgct
    25861 caatagacaa cttctcacct cacgccagat tctggtggcc tttagcggcg ggcttgactc
    25921 caccgttctg ctgcatcagt tggtgcagtg gcggacggaa aatccgggcg tcactctgcg
    25981 cgctattcat gtgcatcacg gtttaagtgc caatgccgat gcctgggtta cgcattgcga
    26041 aaacgtctgc caacagtggc aggtgccgct ggtggtcgaa cgcgtacaac ttgcgcaaga
    26101 ggggctgggg attgaggccc aggcgcggca ggcacgttat caggcatttg cccgtaccct
    26161 attgcccggc gaagtgctgg tcaccgcgca acatctcgac gatcaatgtg aaacctttct
    26221 gctggcgcta aaacgcggta gcggccctgc cgggctttcg gctatggcgg aagtttcgga
    26281 gtttgctgga acacggctta tccgcccgtt gcttgcccgc acgcgggggg aactggagca
    26341 gtgggcattg gcgcatggtt tacgctggat tgaagacgaa agtaatcagg acgactcata
    26401 cgatcgtaac tttctgcgcc tgcgcgtagt gccgttattg cagcagcgtt ggccgcattt
    26461 tgcggaagca acggcccgca gcgccacact ttgtgctgaa caagagagtc tgttggatga
    26521 actgctggcg gatgatttag cacactgcca aacgtcgcag gggacgctgc agattgcgcc
    26581 aatgctggcg atgagtgatg cccgtcgcgc ggcgattatt cgccgctggc tggcagggca
    26641 gaatgcaccg atgccttccc gcgacgcgct ggtgcgcatc tggcaggaag tggcgctggc
    26701 gcgggaagat gcttccccct gtttacgttt gggcgccttt gaaatccgcc gctatcagtc
    26761 gcaactgtgg tggattaaat ccgtcaccgg gcaaagcgaa accattgtgc tgtggcagac
    26821 gtggctacaa ccgctggaat taccggcggg gctgggaacc gtacagctta ctgcgggagg
    26881 cgatattcgc cctccgcgtg cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt
    26941 gctgcatatt gtcgggcgca acggcggacg taagctgaag aaaatctggc aagagttagg
    27001 cgtgccgccg tggctacgtg acaccacgcc actgctgttt tatggagaaa cgctgattgc
    27061 ggcggcaggg gtatttgtga cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt
    27121 tgtctggcag aaaacgctta gttaagtgaa agccggataa gacgcatcaa acgtcgcatc
    27181 cggcgaaagt caatcaggat tcgcttacca ccaccgtacc gatttcaggg tggctaaagc
    27241 tggtaatttt atccagacgc agctcgcggg tttcgccagc ggcctcgacg accagatact
    27301 ccacattttt gcgggagact aaatcactgg cttttgcctg caatttttcg ccatctttca
    27361 gctcaagtgt cagcattaaa tgatgctggc aggcgagctc aagattatcg taatcatcac
    27421 aattaattgg ttgatacgta tcattcattg acataatcgc tcaccagtaa gtttgccgca
    27481 gcgtatgctg ctttttccct gacagcctca gaaagggcgt cgtcggcagc catttcattc
    27541 agcactttca aaacgcagcc cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt
    27601 tccgcgtacc gcttgcgtat taactcacaa tatttttcca catgccctcc tgtcagcact
    27661 ctgacttaac cgtggatgca agtctaagcc tacgaagata aactctgttt cgcaaggtga
    27721 ctataccaca ctcatttctg caatatcagc gccgcaactg cacgtattcc gttacaatgg
    27781 cctcctgatt cgaaaggagt tttcttatgg cgcttaaagc gacaatttat aaagcgacgg
    27841 ttaatgtggc cgatctcgac cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc
    27901 acccttcaga aacccaggag cgtatgatgc tgcgcttgct ggcgtggctg aaatatgctg
    27961 atgaacgtct gcaatttacc cgtggtttgt gtgccgatga tgagccggaa gcgtggctgc
    28021 gtaacgatca cctgggcatt gatttgtgga ttgagctggg gctgccggat gagcgacgaa
    28081 ttaagaaagc ctgcacccag gccgcagaag tggcgctgtt tacctataat agtcgggcgg
    28141 cgcaaatctg gtggcagcaa aatcagagca aatgtgtgca gtttgccaat ctttccgtct
    28201 ggtatctgga cgatgaacaa ctggcgaaag taagcgcctt tgccgatcgt accatgacgc
    28261 tgcaggcaac gattcaggat ggtgttatct ggttatcgga tgataagaat aatctggaag
    28321 ttaacttaac cgcctggcaa caaccttcat gattgtgatt tcccgacatg ttgctattcc
    28381 cgatggtgag cttgagatca ccgccattcg tgcgcagggc gctggcgggc agcatgttaa
    28441 taagacctca acggctattc atctgcgttt tgacattcgg gcgtccagcc tgccagagta
    28501 ttacaaagag cgtctgctcg ccgccagcca tcatttgatc agcagtgatg gcgtgattgt
    28561 cattaaggca caggaatacc gcagtcggga actgaaccgc gaagccgctc tggcccggct
    28621 ggtggcagtg attaaagatt taacaaaaga acaaaaagcc cgacgaccca cgcggcccac
    28681 ccgtgcatcg aaagagcgca ggctggcatc gaaagcacaa aaatcaagcg tgaaggcgat
    28741 gcgcggcaaa gtacgcagcg gtcgggaata aaaagaagga atggatggtg aaaaaagcga
    28801 tagtgacagc gatggctgta atcagcctct ttactctgat gggatgtaat aatcgggccg
    28861 aagtcgatac gctttctccg gcgcaggctg ccgaactgaa accgatgccg caaagttggc
    28921 gcggcgtgct accgtgtgcc gattgcgaag gaatcgaaat ctctctgttc ctcgaaaaag
    28981 acggaacatg ggtgatgaat gagcgttatc tcggtgctcg tgaagaacct tcctccttcg
    29041 cttcctacgg tacatgggcg cgaaccgctg acaagctggt attaaccgac agcaaaggtg
    29101 aaaagtcata ttatcgggcg aaaggcgatg cgctggagat gctcgatcgt gaaggcaatc
    29161 caattgaatc gcagttcaac tatacgctgg aaccggcaca atccagttta cctatgacgc
    29221 cgatgaccct gcgaggcatg tatttttata tggctgatgc ggcgaccttc actgattgcg
    29281 cgaccggaaa acgtttcatg gtagcgaata acgcagagct ggagcgtagc tacctggctg
    29341 cgcgcggtca cagtgaaaaa ccggtgttac tgtcagtaga aggtcacttt acgcttgagg
    29401 ctaatccgga tactggtgcg ccaaccaaag tattagcgcc cgatacggcg ggtaaatttt
    29461 acccgaataa ggattgcagt agtttggggc agtaaacctg cttaaggcag aaacaaacgc
    29521 aaaattgcct gatgcgctgc gcttatcagg cctacagcat ataatgcaac attttgaatt
    29581 agcacgattt tgtaggccgg ataaggcgtt tacgccgcat ccggcatttt gtgcacgagg
    29641 ctggctttat cactgagtca gcccaacaaa gcgacccgcc ttgatataaa tcccaggctt
    29701 caggtgccca acatactgta actgcgtttc tggcttaaac gcagagacat caccaatacg
    29761 catatgcatc agatcggcag ggctaatcca gcccgattgc gccagtgtaa gtggatgccc
    29821 ggatttggca aatgcatcag tgacaaattc cgaacaaaac cacgactttt tgtctccttc
    29881 gcacacactg cttaactgcg ctttcgccag gtcgctgacg cactgttggc gaaaatcctc
    29941 ggagaacgga ttcagtgagc acatctggcg agtcaccata aagggaataa attcgacaat
    30001 gccgcgatag ttataaccgc tatctttgat tttattggca aacgcggtga tatctgtggc
    30061 ttgttgcggg gtaagatccg ggactcgtaa gacgaaaagc ttatcactat gcttcatcgc
    30121 ttttttaagg gaaacaatct ggacgccagc gcctgtcgct tctgcaacgt tattatcacc
    30181 cagaaagatt gcaacgtgac tcacagagga agtgctgaag acgcggattc caaatgaggt
    30241 taccccaagg cttgaggaga acagcaaatc gccgggtttg agatcaggtg ccgttatttc
    30301 ttttattgat tgttcggtga aagagctttg atgctggaat ttgacagccc atgtttttgc
    30361 ctcggcatct actgctgtgg ctgatgggtc tggctggctg atatcaacgg tacaagccga
    30421 aagtaaaaga aagctgggga gaagcaggcg gcagtacgcc tttggtttat ccattttata
    30481 caatccatgt aaaaaagggc cctgaaattc aggacccttt ctggcatcag cctttaatct
    30541 gtttcaccag atattcgacg atgtcaccag tcttaattaa ctgtttctcg ccgttgcgac
    30601 gatatttata ttcgatatcg tcgttgtcga ggttacggtc gcccagcaca atagtgtgcg
    30661 gaataccgat cagttccata tcagcaaaca tcacgcccgg acgctctttg cggtcatcca
    30721 gcagcacttc gataccttgt gcacgcagtt cgctgtacag tttctcagca agctcttgta
    30781 cgcggaagga tttgtgcatg ttcatcggca gaatcgccac ctggaacggc gcgatagcgt
    30841 caggccatac gatgccgcgt tcgtcgtagt tctgctcaat cgccgcagct accacacgcg
    30901 ttaccccgat accgtagcaa cccatcgtca ggatttggtt acggccatct tcaccctgta
    30961 cggaggcttt cagtgcttcg gagtacttgg tacccagctg gaagatgtga ccaacttcga
    31021 taccacgttt gatcagcagc gtaccctggc catccgggct tggatcgcca gccaccacgt
    31081 tacggatatc tgcaacttcc ggggtagcga catcgcgatc ccagttgata ccgaagtagt
    31141 gtttaccatc gatgttagca ccagcagcga aatcactcat cgccgcaacg gtacggtcaa
    31201 tcaccaccgg aatcggcatg tttaccggac ccagtgaacc cggaccggct ttaaccacag
    31261 cacgaatttc ttcttcggtc gcgaaagtca gcgggcttgc aacctgcggc agtttttctg
    31321 ctttaacttc gttcagctcg tgatcaccgc gcaccagcag cgcaaccagc gggaagctac
    31381 tgccttcaac cgctttaacc agcagagtct taaccgtttt ctcaatcggc agattgaact
    31441 gttcaaccag ttccgcgatg gttttcgcgt tcggcgtatc aaccagcgtc atttcctggg
    31501 tagcagcagc gcgcggttct ttcggcgcga tagcttctgc cagttcaatg ttcgctgcat
    31561 agtcagaggt gtcggagaag accacatcgt cttcaccgct ctgcgccagc acctggaatt
    31621 cgtgagaggc gctgccgccg atagaaccgg tgtcggcctg tacggcgcgg aaatccagcc
    31681 ccatgcggct gaagattttg ctgtaggccg catacattgc atcgtaggtt tcctgcaggg
    31741 attcctgaga agtatggaaa gagtaagcat ctttcatcag gaattcgcgg gaacgcatga
    31801 cgccgaaacg cggacgcact tcgtcgcgga acttggtctg gatctgatag aagttcagcg
    31861 gcagctgttt gtaagagcta agctcgttac gaatcaggtc agtgataact tcttcatgag
    31921 ttgggccgag tacgaacgga cgctcgccac ggtcaacaaa acgcagcagt tccggaccgt
    31981 actgttccca acgaccactc tcttgccaca aatcggctgg ctgaaccacc ggcatcgaca
    32041 cctcgatcgc accggcgttg ttcatctctt cacgcacgat gttttcgact tttttcagaa
    32101 cgcgcacgcc ggtcggcagc caggtatata acccggaggc cagcttgcgg atcatcccgg
    32161 cgcgcagcat cagctgatgg ctgatcacct cggcgtcggc aggtgtctcc ttgagagtgg
    32221 agagcaggta ttggctagta cgcatgttgt tacggttcca gttggaaggt agaacaggct
    32281 caaggcgagc ctgggacaaa aaaagtgatt tagtttacca gtgcaaaaga aatgtcaaaa
    32341 gagaagggcg tgaatttaac gcggttccag cgcaaagact tcaaaacctg cgtcggtgac
    32401 gcgccagcga acgttaaaat catgtagcca gacggcatag gttttgcccg tttcctcacc
    32461 tttacgatag gccgggcgcg ggtcctgcgc cagtacttcg cggataaaca gcgttaactg
    32521 cggataacgc ttctccagcg tcaaaagctg cttttcgacc tctgcggtaa aactcaccgc
    32581 catctctgca gctggcgcac tttgcgcata gctggcactg gcatcgggaa gcgattcggc
    32641 aaaggggaga tacggtttga tatccactac cggcgtacca tcgaccagat ccagactgcc
    32701 gagcttcaga atcacgctgc ctttatggca aacaacctct ttcagctcta ccagcgacat
    32761 gccaattggg ttagggcgga aagtagagcg tgttgcgaaa acccccattc tggcgttacc
    32821 gccgaggcgc ggtggacgca cagtcggacg ccagccgcct tccatcgttt gatgaaagac
    32881 gaaaaggatc cataaatggc tgaatgcttc caggccgcga acggcgtcgg cctggttgta
    32941 ggaagctatg agatgcagtt cgccgttggc gctttttacc agacctggct ggcgcggaac
    33001 ggcgaatttt tctttatagg gcgagcgaat aacgcctatt tgctcgaact ggaaactgct
    33061 catttcgccg taatgttaag cgcagagccg atacatacag cctgacgata gcagcccggc
    33121 gtaccgctgg tgacttcgca gctatgcagt aatacagcat tggctttcat tttagaggca
    33181 ttgatttgca tctgcttacg tgcggttgga atgctcggcg gagagtcctg attagaggcc
    33241 tggcaagagt cgccactgac ttcaccgaga tcgcggaacg gtttgccgac taattcttct
    33301 gcattggtat aaattcggac cggcgtggcg cgcggcgctt tcggttttgc aggctccgct
    33361 ttcggctggg gtgcagtgct ttgaacgggt tcgacagggg atctgcttaa catggaacag
    33421 ccgcttagca tgagtgctac taaacagatc ggtaaagcac gcatagtatt tcctcaatgt
    33481 atgatcaaaa cgtcaatatt gaatcaggag cttgtaaaaa tgacaagacg ggcaagcgcc
    33541 cgtcctgaat gatattacaa attgtggaaa cagcctaaaa attaccagcc tttaacagcg
    33601 ccgccgttaa acactttgtt tgctgcttcg taaacttcat cagactgata agcctggacg
    33661 aatttcttca cgttctcggc gtctttgtta tcttcacgcg ttacgatcag gtttacgtat
    33721 ggggagtctt tatcttcgac aaagataccg tctttcgccg gagtcaggcc aatctggctg
    33781 gcataggtgg tattgataac tgccagagcg atttgcgcgt cgtccagaga acgcggcagt
    33841 tgcggtgctt ccagttcaac aattttcaga ttttttgggt tctcaacaac atcaagaacg
    33901 gtcggcagca ggccaacgcc atctttcagt ttgatcaagc ccactttttg cagcagcagc
    33961 agtgaacgac caagattagt tggatcgttt ggcacggcaa cctgcgaacc atcctgcagt
    34021 tcatccagtg atttgatttt cttggagtaa ccagcaatcg gataaacaaa agtgttgcct
    34081 actgcgacca gtttgtagcc acgatctttc agttgctgat caaggtacgg tttatgctgg
    34141 aaggcgttgg cgtcgatatc gcctttgctc aatgcttcgt ttggcagaac atagtcgttg
    34201 aaggttacca gctcaacgtc caggccatat ttgtctttcg caactttctg cgcgacttct
    34261 gctacctgct gttcggcacc aacaatcacg ccgactttaa tgtggtttgg atctttttca
    34321 tcctgaccgc agcctaccag tgccagtgat ccaatcaggg ctcccactgc cgcaaaggtt
    34381 ttgaatttga acgccatacc ttattccttc ttcaattatt tatgttgtgt tgaacgttac
    34441 ttgcgagtga cagcccggac gatgcggtcg cctgcaaact gaattaaata aaccagaatg
    34501 accagcaata ccagtaccgt attcatcacc gtcgcgttgt agccgatgta gccatactga
    34561 tagccaatct gacctaaacc accggcaccg actgcaccac ccatcgcgga ataaccgacc
    34621 agggtaatca gggtgatagt tgccgcattc accagacccg gcaacgcttc cggtaacagc
    34681 actttacaga cgatctgcat tggcgtggca cccattgcgc gggaagcttc aattaacccg
    34741 gttgggatct ccagcagagc gttctcgacc atacgggcaa taaacggtgc tgcaccaacg
    34801 gttaacggaa caattgctgc ctgcaaacca atcgatgtac cgacaataac gcgggtaaac
    34861 ggaatcatcc ataccagcaa gataatgaac gggatggaac ggaaaatgtt cacaatcgca
    34921 gaaacggtac gatacagctt cgcgttagca ataatttgcc ccggacgcgt gacataaagc
    34981 agaacgccaa ccggcagacc aatcacaaac ccaaaaaatc cggatacgaa ggtcattgcc
    35041 agcgtttccc atacgccacg aaccagcagc cacatcatcg gctcagacat aacccagtac
    35101 ctctactttt acatggtgtt cctgcagcca ggcaatggcg gcttgcgtat cttgttgtgt
    35161 gccgtgcatt tcagtcagca tgatgccgaa tttaacgcca ccggcgtagt ccatctgcgc
    35221 gctaataatg ttgttgttga cgttgaaacg acgcgcggtt tcagaaagca gtggggcatc
    35281 gaccgattga ccggtaaact ccagacgcag catcggcacg cagtcagtaa atggctccgt
    35341 ttgcagacgt tcctggtaat cttccgggat atccagatgc agggtcgact gaataaactt
    35401 ctgcgccagc ggcgttttcg gatgcgagaa cacttcactt accgtgtcct gctcgatcag
    35461 ttcgccattg ctgatgaccg ccacgcaatc acaaatacgc ttcacaacgt ccatttcgtg
    35521 ggtgatcaac agaatcgtca aacccagacg gcggttaatg tctttcagca gttcgagaat
    35581 agaacgtgtc gttgccgggt ccagcgcgct ggtggcttca tcacacagca atactttggg
    35641 attgctggct aatgcacggg caatcgccac acgttgtttc tgcccaccgg aaagattcga
    35701 cgggtagcta tcatgcttat cgccaagacc aaccaatgac agcaattccg tcacgcgacg
    35761 tttgatctcg tctttcggtg tgttgtccag ctccagcggc agagccacgt tgccaaaaac
    35821 agtacgcgaa gagagcaggt taaaatgctg gaaaatcata ccaatctggc ggcgagcttt
    35881 ggtcaactcg gattctgaca gcgtggtcaa ttcctggcca tcgaccagca cgctaccctc
    35941 ggttgggcgc tccagcaagt ttacacaacg tataagcgta ctcttacccg cgcctgaggc
    36001 accgataacg ccataaattt gtccagctgg cacatgcagg ctgacgttgt tcaacgcctg
    36061 gatggtgcgg gtgccctggt ggaacacttt ggtgatattc gaaagtttta tcattgatta
    36121 tttattatcg tcattaagtt agtcgtagca tctcgaatgc ctgaaacggg caacgccgtc
    36181 aatgaaatgg atgttaaggc atccagacgt ctaaatcaat caggtttatg cgaagagcac
    36241 tttcttgcag gtcgaaacat gcgatactag cgtcccatgc cttattaagg agctataaaa
    36301 ggtggcgaag agcgtacccg caatttttct tgaccgtgat ggcaccatta atgtcgatca
    36361 cggctatgtc catgagatcg acaactttga atttatcgac ggtgttattg acgccatgcg
    36421 cgagctaaaa aaaatgggct ttgcgctggt ggtagtaacc aaccagtctg gcattgctcg
    36481 cggtaaattt accgaagcac agtttgaaac gctgaccgag tggatggact ggtcgctggc
    36541 ggaccgagat gtcgatctgg atggtatcta ttattgcccg catcatccgc aggggagtgt
    36601 tgaagagttt cgccaggtct gcgattgccg caaaccacat ccggggatgc ttttgtcagc
    36661 acgcgattat ttgcatattg atatggccgc ttcttatatg gtgggcgata aattagaaga
    36721 tatgcaggca gcggttgcgg cgaacgtggg aacaaaagtg ctggtgcgta cgggtaaacc
    36781 tattacacct gaagcagaaa acgcggcaga ttgggtgtta aatagcctgg cagacctgcc
    36841 gcaagcgata aaaaagcagc aaaaaccggc gcaatgatta aaagatgaac ggttgaaata
    36901 aaaatgcatt tttccgcttg tcttcctgag ccgactccct ataatgcgcc tccatcgaca
    36961 cggcggatgt gaatcacttc acacaaacag ccggttcggt tgaagagaaa aaatcctgaa
    37021 attcagggtt gactctgaaa taggaaagtg tgatatacgc cacctcgcga cagtgagctg
    37081 aaagctgcgt cgcaactgct ctttaacaat ttatcagaca atctgtgtgg gcactcgaag
    37141 atacggattc ttaacgtcgc aagacgataa atgaatacca agtctcaaga gtgaacacgt
    37201 aattcattac gaagtttaat tctttgagca tcaaactttt aaattgaaga gtttgatcat
    37261 ggctcagatt gaacgctggc ggcaggccta acacatgcaa gtcgaacggt aacaggaa
//
LOCUS       DAZSNM010000014        35222 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZSNM010000014 DAZSNM010000000
VERSION     DAZSNM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35222
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.014"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1224
                     /locus_tag="ACHW42_000648"
     CDS             <1..1224
                     /locus_tag="ACHW42_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR8942602.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="glpX"
                     /locus_tag="ACHW42_000649"
     CDS             1221..2186
                     /gene="glpX"
                     /locus_tag="ACHW42_000649"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR8942603.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="ACHW42_000650"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="ACHW42_000650"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFR8942604.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="ACHW42_000651"
     CDS             2714..3427
                     /locus_tag="ACHW42_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR8942605.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="ACHW42_000652"
     CDS             complement(3399..4076)
                     /locus_tag="ACHW42_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8942606.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="ACHW42_000653"
     CDS             complement(4070..4747)
                     /locus_tag="ACHW42_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8942607.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5442)
                     /locus_tag="ACHW42_000654"
                     /pseudo
     CDS             complement(4735..5442)
                     /locus_tag="ACHW42_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            complement(5443..6021)
                     /locus_tag="ACHW42_000655"
     CDS             complement(5443..6021)
                     /locus_tag="ACHW42_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR8942608.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="ACHW42_000656"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="ACHW42_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="ACHW42_000657"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="ACHW42_000657"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR8942609.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="ACHW42_000658"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="ACHW42_000658"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR8942610.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHW42_000659"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHW42_000659"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR8942611.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="ACHW42_000660"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="ACHW42_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR8942612.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="ACHW42_000661"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="ACHW42_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR8942613.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="ACHW42_000662"
     CDS             12291..13031
                     /locus_tag="ACHW42_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR8942614.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="ACHW42_000663"
     CDS             13198..14148
                     /locus_tag="ACHW42_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8942615.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHW42_000664"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHW42_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHW42_000665"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHW42_000665"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR8942616.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHW42_000666"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHW42_000666"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR8942617.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHW42_000667"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHW42_000667"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR8942618.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHW42_000668"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHW42_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR8942619.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHW42_000669"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHW42_000669"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR8942620.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="ACHW42_000670"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="ACHW42_000670"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR8942621.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHW42_000671"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHW42_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR8942622.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="ACHW42_000672"
     CDS             23768..23827
                     /locus_tag="ACHW42_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR8942623.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="ACHW42_000673"
     CDS             complement(23928..24530)
                     /locus_tag="ACHW42_000673"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR8942624.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="ACHW42_000674"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHW42_000674"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHW42_000675"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHW42_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR8942625.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHW42_000676"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHW42_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR8942626.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="ACHW42_000677"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="ACHW42_000677"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR8942627.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHW42_000678"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHW42_000678"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR8942628.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHW42_000679"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHW42_000679"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR8942629.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..29759)
                     /locus_tag="ACHW42_000680"
     CDS             complement(29676..29759)
                     /locus_tag="ACHW42_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942630.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30050..31144
                     /gene="gcvT"
                     /locus_tag="ACHW42_000681"
     CDS             30050..31144
                     /gene="gcvT"
                     /locus_tag="ACHW42_000681"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR8942631.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPSAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31168..31557
                     /gene="gcvH"
                     /locus_tag="ACHW42_000682"
     CDS             31168..31557
                     /gene="gcvH"
                     /locus_tag="ACHW42_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR8942632.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31676..34549
                     /gene="gcvP"
                     /locus_tag="ACHW42_000683"
     CDS             31676..34549
                     /gene="gcvP"
                     /locus_tag="ACHW42_000683"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR8942633.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34816..>35222
                     /locus_tag="ACHW42_000684"
     CDS             34816..>35222
                     /locus_tag="ACHW42_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFR8942634.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGA"
BASE COUNT         8397 a         8835 c         9565 g         8425 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caagcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttagtgtt ttttgacatt
    29761 tttttagctc ttaatattgt cttattcaaa ttgactttct catcacatca tctttgtata
    29821 gaaattggtg tattttttgg ttttttattc tgtcgcgatt tttgcatttt ttaaccataa
    29881 gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg
    29941 caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt ttgtggtgat
    30001 aatttcacca tgaaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    30061 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    30121 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    30181 cggaatgttt gatgtgtcgc atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    30241 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    30301 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gatctcatcg tctactactt
    30361 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    30421 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    30481 gattgccgtg caagggccga gtgctcaggc aaaagctgcc acactgttta atgatgctca
    30541 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggtgggcg atctgtttat
    30601 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    30661 ggccgatttc tggcgtgcgc tggtggaagc gggcgttaag ccctgcggtc tgggggcgcg
    30721 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    30781 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccgacag atcgtgactt
    30841 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    30901 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    30961 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    31021 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    31081 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    31141 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    31201 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    31261 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    31321 gttagcgcgg gcgatgactg cgcggttgcc gaatcggtaa aagcggcgtc agacatttat
    31381 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc cccggaactg
    31441 gtgaacagcg aaccgtatgc aggcggctgg atctttaaaa tcaaagccag cgatgaaagc
    31501 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga tgagtaacgg
    31561 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    31621 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    31681 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    31741 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggtgca caatcgttaa acgcgctgac
    31801 cggccagatt gtgccgaaag atattcagct tgcgacacca ccgcaggttg gcgcaccggc
    31861 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    31921 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    31981 gctggaaaat ccgggctggt ataccgcgta cactccgtat cagcctgaag tctcccaggg
    32041 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    32101 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    32161 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccaatg tgcatccgca
    32221 aacgctggat gtggtccgta ctcgtgctga aacctttggt tttgaagtga ttgtcgatga
    32281 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    32341 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    32401 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    32461 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    32521 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    32581 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    32641 gcaacatatt cgtcgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    32701 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    32761 ccgcattcac cgtctgaccg atattctggc ggcgggcctg caacaaaaag gtctgaaact
    32821 gcgccatgcg cactatttcg acaccttgtg tgtggaagtg gccgacaaag cgggcgtact
    32881 gacgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg ccgtggggat
    32941 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagtg tgctgctggg
    33001 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    33061 tatccagcct gcgatgctgc gcgacgacga aatcctcacc catccggtgt ttaatcgcta
    33121 tcacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    33181 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    33241 gatcccaatc acttggacgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    33301 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    33361 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    33421 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    33481 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    33541 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    33601 gggcgataac ctctcctgta tcatggtgac ttatccttct acccacggcg tgtatgaaga
    33661 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    33721 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    33781 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    33841 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    33901 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    33961 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    34021 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    34081 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    34141 gaaagaagaa accggcatca gcgagctgga cattgccaag cgcctgatcg actacggttt
    34201 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    34261 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    34321 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    34381 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    34441 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    34501 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa taccagtaat tcactgattc
    34561 gagtattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    34621 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    34681 gcgcgaatat ttaccctcac cccgaccctc tccctgaaag ggcgaggggg aaaagcgtgc
    34741 caacattgaa gattgagcca gtttgttagc aatctcaaag atacgtcaac gaattgattt
    34801 ttctcggaaa aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg
    34861 gcaactgcat tactgttggc gcaagaaggg tatacggtgg cggttaatta tcagcaaaac
    34921 ctccacgcgg cgcaggaagt gatgaactta ataatgcaag ccggtggcaa agcattcgtg
    34981 ctccaggcgg atatcagcga cgaaaaccag gtcgttgcga tgtttacagc aatcgatcag
    35041 cacgatgaac cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc
    35101 gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat
    35161 tttctctgct gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc
    35221 gc
//
LOCUS       DAZSNM010000015        31904 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZSNM010000015 DAZSNM010000000
VERSION     DAZSNM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31904
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.015"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..299
                     /locus_tag="ACHW42_000685"
     CDS             <1..299
                     /locus_tag="ACHW42_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR8942635.1"
                     /translation="RQGGTTTDEGERIDYRVKMYNPETGGQIDVRNNENMVWNSINLK
                     RVRPVVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(387..1685)
                     /gene="exuT"
                     /locus_tag="ACHW42_000686"
     CDS             complement(387..1685)
                     /gene="exuT"
                     /locus_tag="ACHW42_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFR8942636.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            2170..3582
                     /gene="uxaC"
                     /locus_tag="ACHW42_000687"
     CDS             2170..3582
                     /gene="uxaC"
                     /locus_tag="ACHW42_000687"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR8942637.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            3597..5084
                     /gene="uxaA"
                     /locus_tag="ACHW42_000688"
     CDS             3597..5084
                     /gene="uxaA"
                     /locus_tag="ACHW42_000688"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR8942638.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            5167..5718
                     /locus_tag="ACHW42_000689"
     CDS             5167..5718
                     /locus_tag="ACHW42_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR8942639.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(5723..6967)
                     /gene="sstT"
                     /locus_tag="ACHW42_000690"
     CDS             complement(5723..6967)
                     /gene="sstT"
                     /locus_tag="ACHW42_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR8942640.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(7563..8528)
                     /gene="alx"
                     /locus_tag="ACHW42_000691"
     CDS             complement(7563..8528)
                     /gene="alx"
                     /locus_tag="ACHW42_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR8942641.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(8799..9797)
                     /locus_tag="ACHW42_000692"
     CDS             complement(8799..9797)
                     /locus_tag="ACHW42_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR8942642.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(9876..10568)
                     /locus_tag="ACHW42_000693"
     CDS             complement(9876..10568)
                     /locus_tag="ACHW42_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR8942643.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(10645..11148)
                     /locus_tag="ACHW42_000694"
     CDS             complement(10645..11148)
                     /locus_tag="ACHW42_000694"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR8942644.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            11233..12369
                     /gene="rlmG"
                     /locus_tag="ACHW42_000695"
     CDS             11233..12369
                     /gene="rlmG"
                     /locus_tag="ACHW42_000695"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR8942645.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            12654..12968
                     /gene="higB"
                     /locus_tag="ACHW42_000696"
     CDS             12654..12968
                     /gene="higB"
                     /locus_tag="ACHW42_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR8942646.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            12965..13381
                     /gene="higA"
                     /locus_tag="ACHW42_000697"
     CDS             12965..13381
                     /gene="higA"
                     /locus_tag="ACHW42_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR8942647.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(13426..15444)
                     /gene="fadH"
                     /locus_tag="ACHW42_000698"
     CDS             complement(13426..15444)
                     /gene="fadH"
                     /locus_tag="ACHW42_000698"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR8942648.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(15670..18021)
                     /gene="ygjK"
                     /locus_tag="ACHW42_000699"
     CDS             complement(15670..18021)
                     /gene="ygjK"
                     /locus_tag="ACHW42_000699"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR8942649.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(18038..19109)
                     /gene="ygjJ"
                     /locus_tag="ACHW42_000700"
                     /pseudo
     CDS             complement(18038..19109)
                     /gene="ygjJ"
                     /locus_tag="ACHW42_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(19243..20676)
                     /locus_tag="ACHW42_000701"
     CDS             complement(19243..20676)
                     /locus_tag="ACHW42_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8942650.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(20739..21188)
                     /locus_tag="ACHW42_000702"
     CDS             complement(20739..21188)
                     /locus_tag="ACHW42_000702"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR8942651.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(21185..24271)
                     /gene="ebgA"
                     /locus_tag="ACHW42_000703"
     CDS             complement(21185..24271)
                     /gene="ebgA"
                     /locus_tag="ACHW42_000703"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFR8942652.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(24454..25437)
                     /gene="ebgR"
                     /locus_tag="ACHW42_000704"
     CDS             complement(24454..25437)
                     /gene="ebgR"
                     /locus_tag="ACHW42_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR8942653.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            25655..25986
                     /locus_tag="ACHW42_000705"
                     /pseudo
     CDS             25655..25986
                     /locus_tag="ACHW42_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(26028..27407)
                     /gene="ygjG"
                     /locus_tag="ACHW42_000706"
     CDS             complement(26028..27407)
                     /gene="ygjG"
                     /locus_tag="ACHW42_000706"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR8942654.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            27825..29345
                     /gene="aer"
                     /locus_tag="ACHW42_000707"
                     /pseudo
     CDS             27825..29345
                     /gene="aer"
                     /locus_tag="ACHW42_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(29499..30122)
                     /gene="nfeR"
                     /locus_tag="ACHW42_000708"
                     /gene_synonym="yqjI"
     CDS             complement(29499..30122)
                     /gene="nfeR"
                     /locus_tag="ACHW42_000708"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR8942655.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            30399..31144
                     /gene="nfeF"
                     /locus_tag="ACHW42_000709"
                     /gene_synonym="yqjH"
                     /pseudo
     CDS             30399..31144
                     /gene="nfeF"
                     /locus_tag="ACHW42_000709"
                     /gene_synonym="yqjH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
     gene            complement(31198..31273)
                     /locus_tag="ACHW42_000710"
     tRNA            complement(31198..31273)
                     /locus_tag="ACHW42_000710"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31238..31240),aa:Ile,seq:cat)
     gene            31398..31904
                     /gene="mug"
                     /locus_tag="ACHW42_000711"
                     /pseudo
     CDS             31398..31904
                     /gene="mug"
                     /locus_tag="ACHW42_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         7703 a         8325 c         8401 g         7475 t
ORIGIN      
        1 atcgtcaggg agggaccacc accgacgagg gagaacgcat tgattaccgg gtcaaaatgt
       61 acaacccgga aaccggtggg caaattgatg tgcgcaataa tgaaaatatg gtctggaaca
      121 gcattaacct gaaacgtgtg cgtccggtgg tactgcccgg tattcgctat gccgtgatgt
      181 gtgtgccgac acccttgact ctggcagtcg ataaattcag cgtgatggat aaacaggccg
      241 ggtactacat gggcaaattg tcagtaatct ttacgccttc cttgccaacc atcaattaac
      301 cctgcaaacc ggacaaacgc ttttttctat ataaacgacg aagccgcctg ataaggcggc
      361 ttcgtcgtta taacttacgt taaacgttaa tgttgcggtg cgggatcgtt atgtgtttcc
      421 tgcgccacct caatcgccgg tttgttctgc aatacggtcc agataaccag cgcacccagc
      481 aggtcaaata ctgccagaac tgcgaacagc gggctgaagc cgatggtgtc agccagtgca
      541 ccgacaacca gcgcaaacag cgtacttgcc agccatgcgg acatcccggt taaaccgttc
      601 gccgtcgcca cttcgttacg accgaacacg tcagaagaga gcgtaatcag cgcgccagac
      661 agtgcctggt gggcaaaacc accgatacac agcagcataa ttgcgacata cgggttggtg
      721 aacagaccga tcatacccgg gccaatcatc agcacagcac ccagcgttac gaccatctta
      781 cgggaaacga tcaggttcac accaaaccaa cgctggaaca gcggcggcag gtaaccaccg
      841 aggatacaac cgaggtcagc aaacagcatc ggcatccagg cgaacatcgc gatctctttc
      901 aggttaaagc cgtaaacttt aaacatgaac agcgggatcc acgcgttaaa agtaccccag
      961 gccggttctg caaggaaacg cggcagtgcg ataccccaga actgacggtt acgcaggatc
     1021 tgaccaacgg acattttctt cgccgtgctc acctggtgct gggcttcctg accattaata
     1081 atatagtcgc gttcttcatc ggtcagatgc ttctggtcgc gcggatgttt atagaaaatc
     1141 agccatgcca tcgcccagat aaagctcaat gcaccggaga tgataaatgc catctgccag
     1201 ctgtgcatca cgattgccca taccaccagc ggcggcgcaa tcatcgcacc aatcgaagaa
     1261 cctacgttaa agtagcctac tgcgatggaa cgctctttcg ccgggaacca ttcggagctg
     1321 gctttcagac ccgccgggat catcgctgct tccgcggcac cgaccgcacc acgagcaaca
     1381 gctaggccac cccagctacc tgccagcgcg gttgcaccac agaacacggc ccacagcaca
     1441 gcaaacattg cataaccgat tttcgtaccc agcacatcca gtacataacc tgctaccggt
     1501 tgcatgaccg tataagcagc agaataggct gcgatgatat aggaatactg ttgggtggag
     1561 atgtttaact cttccatcag agttggcgca gctgccgcca cagtgttacg cgtcaggtaa
     1621 ccaagcacgg tgccgagcgt caccagtgcg atcatatacc aacgtaaccc tttaatttta
     1681 cgcatgtaaa acctcatcgt tttgttatat ccgcaggaag cgcggccatc atcccgccag
     1741 aggctgaaga tgttagttac gaccgggtaa ccgaaaaaaa aaaccggcac gccccgaacg
     1801 ttgccatgaa agtttcgtgc taattcggct tccgtaccgg ttaacccgat tcatctaaca
     1861 aatgaatgaa tcggcaattt gcattccgtc gacttatgat ttgcgacggc agaaagataa
     1921 cttgtcatac aaccttaaaa ggtgagagcc attacaaatg tgcgaatatt tgtagggaca
     1981 ttacctgacg acagcgaggc cagtactggc gcggcctgca gcgagattta ccactttgag
     2041 agtaattttt ttaactacgt ttattgatct aactcacgaa aatatcttcg gactctggaa
     2101 attggtgtga taactttgtc agcatcgcac cataagcaag ctagctcact cgttgagagg
     2161 aagacgaaaa tgactccgtt tatgactgaa gatttcctgt tagataccga atttgcccgc
     2221 cgtctgtatc acgactacgc aaaagaccag ccgattttcg attaccattg ccatttgccg
     2281 ccgcagcaga ttgcggaaga ctatcgtttt aaaaacctgt atgacatctg gttgaagggc
     2341 gatcactaca aatggcgcgc tatgcgtacc aacggtgtgg ctgagcgtct gtgtactggt
     2401 gatgcgtctg accgtgaaaa atttgacgcc tgggcggcga ctgttccgca cactatcggc
     2461 aacccgttat accactggac gcacctcgaa ctgcgtcgtc cgttcggtat cactggcaaa
     2521 ttgctttctc cgtcaactgc cgatgaaatc tggaacgaat gtaacgaact gctggcgcag
     2581 gataacttct ccgcgcgcgg catcatgcag cagatgaacg tgaaaatggt cggcaccacc
     2641 gatgacccga tcgattctct ggagcatcac gcagagatcg ccaaagacgg ctctttcacc
     2701 atcaaagtgc tgccgagctg gcgtccggac aaagccttca atatcgaaca ggcgaccttt
     2761 aacgactaca tggcgaagct gggcgaagtt tccgataccg acattcgccg ctttgctgac
     2821 ctgcaaactg ccttgaccaa acgtctggat cacttcgccg ctcacggctg taaagtgtct
     2881 gaccatgcgc tggacgtggt gatgtttgct gaagcgaacg aagcggaact ggacagcatt
     2941 ctggcgcgcc gtctggctgg cgaaaccctg agcgagcacg aagtggcaca gttcaaaact
     3001 gcggtactgg tgttccttgg tgccgaatat gcacgtcgcg gctgggtaca gcagtaccac
     3061 attggcgcgc tgcgtaataa caacctgcgt cagttcaaac tgctggggcc ggatgtaggc
     3121 tttgactcca tcaacgaccg tccgatggcg gaagagttgt ctaagctgct gagcaagcag
     3181 aacgaagaaa acctgctgcc gaaaaccatt ctctactgcc tgaacccgcg cgataacgaa
     3241 gtgctgggca ccatgatcgg taacttccag ggcgaaggta tgccgggcaa aatgcagttc
     3301 ggttccggct ggtggtttaa cgatcagaaa gacggtatgg aacgtcagat gacccaactg
     3361 gcgcagctcg gtctgctgag ccgctttgtc ggtatgctga ctgacagccg tagcttcctg
     3421 tcatacaccc gtcacgaata cttccgccgc attctgtgcc agatgatcgg tcgctgggtg
     3481 gaagcgggcg aagcaccggc ggacatcaac ctgctgggcg agatggtgaa aaatatttgc
     3541 tttaacaatg cgcgtgacta cttcgccatt gaactgaact aaggtctggg gttgatatgc
     3601 aatacatcaa gatccatgcg ctggataacg tcgcggtcgc tttagcagat ttggctgaag
     3661 gcacagaagt cagtgtcgat aaccagactg ttacgctgcg ccaggatgtt gctcgtggac
     3721 ataaatttgc gttaacggat atcgcaaaag gggccaacgt catcaaatat ggcctgccga
     3781 ttggttatgc attggcggat attgcggcgg gggaacacgt ccacgcccac aatacgcgca
     3841 cgaatctgag cgatctggat cagtatcgct atcaacttga ttttcaggat ctgcctgcgc
     3901 aagcggcaga tcctgaagtg cagatctatc gtcgcgctaa cggcgatgtc ggggtgcgta
     3961 acgagctgtg gatcctgcca accgtgggct gtgtcaacgg catcgcgcgg caaatccaga
     4021 accgtttcct gaaagagacc aacaacgccg aaggtaccga cggcgtgttc ctcttcagcc
     4081 acacctatgg ctgctcacaa ctgggcgacg atcacatcaa cacccgcacc atgctgcaaa
     4141 acatggtgcg ccacccgaac gcgggcgcag tgctggtgat tggtctgggc tgtgaaaaca
     4201 accaggttgc cgcattccgt gaaacgttgg gcgatatcga tcctgaacgc gttcatttca
     4261 tgatctgcca acagcaggat gatgagatcg aagccgggat cgagcatttg catcagctgt
     4321 ataacgtgat gcgcaacgat aaacgcgagc caggcaaact cagcgaactg aagtttggtc
     4381 tggagtgcgg tggttctgac ggtctttctg gtattactgc taacccgatg ctggggcgtt
     4441 tctctgacta cgtgattgct aacggcggta ctaccgtact gaccgaagtg ccggagatgt
     4501 ttggcgcaga gcagttgctg atggaccatt gccgcgacga agcaacgttt gaaaaactgg
     4561 tcaccatggt caacgacttc aaacagtact ttattgccca tgatcagccg atctacgaaa
     4621 acccatcacc ggggaacaaa gcgggcggta tcaccacgct ggaagacaaa tcacttggct
     4681 gtacccagaa agcgggttcc agtgtcgtgg ttgacgtgct gcgttacggc gagcgtctga
     4741 aaacgccagg gctgaacttg ttaagtgcgc cgggtaacga tgccgtagcg accagcgccc
     4801 tggcgggtgc gggctgccat atggtgctgt tcagtactgg tcgcggcacg ccatacggtg
     4861 gatttgtgcc gacggtgaaa atcgccacca acagtgaact ggcggcgaag aaaaaacact
     4921 ggatcgactt tgacgcgggt cagctgatcc acggtaaagc gatgccgcag ttgctggaag
     4981 aatttatcga caccatcgtt gagtttgcca acggtaagca aacctgcaac gagcgtaacg
     5041 acttccgcga actggcgatc ttcaaaagcg gcgtaacgct ataaagtcga aatgagttgt
     5101 taataaaaaa cggcgtttca taatgtgaag cgccgtttgc ttttccatac ctgctaagga
     5161 tttatcatga ccgcgtattg gctggcccag ggcgtgggtg tcatcgcctt tctgattggt
     5221 atcacaacat ttttcaatcg tgacgaacgt cgcttcaaaa agcagctttc ggtctatagc
     5281 gccgttattg gcgtacattt ttttcttctg ggcacctatc ccgctggtgc cagtgccatc
     5341 cttaatgcca ttcgtacatt gattacctta cgcacgcgca gcttatgggt aatggcgatt
     5401 tttattgtgc tgactggcgg aattggcctc gcgaagttcc atcatcctgt cgaactattg
     5461 ccggttatcg gtacgattgt cagtacctgg gcgctgttcc gctgtaaagg gctcaccatg
     5521 cgctgcgtga tgtggttttc aacgtgttgc tgggtgattc acaacttctg ggcggggtcg
     5581 ataggcggca cgatgattga gggtagtttt ctgcttatga atggcctgaa tatcattcgt
     5641 ttctggcgga tgcagaaaag gggaattgat ccgtttaaag tagagaaaac ccctcccgcc
     5701 atagacgaaa ggggttaaac aattaattac gcagggcgct atttgccaga cgatcgtctt
     5761 ccgcctggca agctgccgca gtgaacagca cgtcagttga agagttcagc gcggtttcgc
     5821 aagagtcctg caatacgccg ataataaagc cgacggcaac cacctgcatg gcgatatcgt
     5881 tcgaaatacc gaacatatta caggccagtg ggatcagcag caaagaaccc cccgccacgc
     5941 cggatgcgcc acaggcacac agagaagcca ctacgctcaa cagcagcgct gtgggcagat
     6001 cgaccggaat acccagcgta ttaaccgcag ccagcgtcaa cacggtaatg gtgattgctg
     6061 cgcccgccat attgatggtg gctcccagcg gaatagaaac tgaataggta tcgcgatcca
     6121 gattcagctt ttcacacagc gccatattca ccggaatgtt cgctgcagag ctgcgggtga
     6181 agaaggcata cacaccgctt tcgcgcaggc acagcagcac cagcgggaac gggttacgac
     6241 gaattttcca ccacaccagc aatgggttaa ccaccagcgc caccagtaac atacagccaa
     6301 ccagcacgac cagcagttgc gcgtagcccc acagtgtgga gaaaccggtg gttgccaggg
     6361 tagaagaaac cagcccaaaa ataccgaacg gtgcgaagcg aatgaccagt ttcaccataa
     6421 aggtaacggc attcgacata tcattgacca ggtttttggt ggtctcgtta ccgtgacgca
     6481 gtgcgaagcc gaggccgatc gcccacacca gaatcccgat gtagttacct ttcagcaggg
     6541 cgtcgatggg gttggaaacc atgctcatta ccagtccgcg catcacttcg acaatgcctg
     6601 acggcggcga aatatcaccc gcgctactgg acaagtgcag ggtagaaggg aaggcaaagc
     6661 tgaagactac tgcggccaga gctgcagaga aggtgcccag cagatagagg aacaaaatag
     6721 gacggatatt ggttttctgc ccgtgctggt ggttagcaat agatgccatt accagcatca
     6781 acaccaggat gggggcaaca gctttcagtg cgccgacgaa caaagtacct aacagaccaa
     6841 cagcttccgc cgcgggtttt gagatccatg ccagaagaat ccccagaaca aggccgacca
     6901 ggatttgttt taccaggctg ccatgagcca gacgccggaa tagccccggt gaacgttgcg
     6961 tagtcatttt tcgatccttt cattgtgttg tcgcacatcc ctggtgcgtt ctgtcgacgc
     7021 atctttcagg atgtaacaaa gtgtttgcaa ggtcgagtat aaggaaagtt acagcttcgg
     7081 gaagcgaaaa atgctggatt ttaaagagag gtcatgtttt gcaacttaat ggttgcattt
     7141 atttaacata aacagaggga aggactggct actaaggctg attagataac atatgggcaa
     7201 aactatttgg aatatgagaa cggttgcgct cctttaacgt gttatgccat ataaagtcac
     7261 tggcgcaggg agcgcgcagg gggcggccaa tcgccgccgc cccctgcacc cccaggctct
     7321 ggcgaacaaa atcgccgctg cgcggttccc tcagcctatc ccttgcggct accgggtcgg
     7381 gcgcaaggta acgtccctgt aagatgcgcc ctcgccccgg cctccaggga acgcttcggc
     7441 gattttgacg ccaccaacac ccgtgcggtc tttttgtaaa gagctataag ccgattacat
     7501 aattaatagc tcacctttgt ctggaatgag ttatctctag gaatggctta atatacagcg
     7561 aatcattctc cccgctgctt atcatgccga taattcaccc aggcgttgat aataaacgtc
     7621 atcaccagaa tgccaaacac cacgcccagc gagacggcga ttggaatatg gtagaagtcg
     7681 acaatcagca tcttgatacc gataaacacc agaatcaccg ccaggccata tttgagcatc
     7741 gagaaacgct ctgctacgcc cgccagcagg aaatacatcg cacgcaggcc gaggattgca
     7801 aacaggtttg aggtcagcac aatgaacggg tcagtggtca cggcgaagat agccggaatg
     7861 ctatccacgg caaaaatcac gtcgctcaat tccaccagaa tcagtaccag catcagcggt
     7921 gtggcgtaca gcaatccatt cttacgcaca aagaaatgct cgttgtcgat ggtgtcggtc
     7981 atgcgcaaat gaccgcgtag ccagcgcacc agcggcttgt cgccaatgcc tgattcatct
     8041 tcatgggcga gcgccatctt cacgccggta aacagcagga aggcaccgaa gatatacagg
     8101 atccagtcga actgcgaaat cagccagctg cctgtgaaga tcatgatggt acgcagaaca
     8161 atcgccccga gcacgccata caccagcacg cgacgttgta atgccgccgg aacggagaaa
     8221 tagctgaaca acatcagcca gacaaagacg ttatcgaccg ccagcgattt ctcaatcaga
     8281 taacccgtga gaaaggccag ggcctgtgga tcggcaacgg cgcgaccttc ggtttgcacc
     8341 agataccacc agaaagcggc gttaaacagt aacgacagcg tcacccagac cagcgaccag
     8401 gccgcagcct gtttcatggt catggcatgt gccccacgac gcccctgcaa caacaggtcg
     8461 atagccagca taatggcgac aacaacagcg aatccgcccc atagcaacgg cgtgccgaca
     8521 gtattcatag aagttcctta cacataaaaa aacggccaac gtcggaagac gtcagccgct
     8581 gctttttatg catagacctc gccttccggc aaggtctcac ttacaacaaa aaaggaatac
     8641 gtctgcgtat tcctttcggg ttgcccggtg accggatgtg gtttttcact catcgtaatg
     8701 acgatcgacc ggcaatgaag ttactcccct ttgcaggtaa caaagtatga cagaccattc
     8761 agtcagtcaa tggtctgtta catacctttt acactgtttt actttggcaa gtatagtttt
     8821 acgctatctg ccggaaaaat caccccagtt tggcggcgga tctcggtcag cagtttggct
     8881 gtgatgcgac tgaccgccag ccctggatga tccaccagat gctcatccac cagggtagcg
     8941 aacagctctg cttcgtagag catggtatta atatgctgcg gctgggtgag atcctgcatc
     9001 tggctgccac gcggcacaaa gcagactttc tggcattcag acagtttttc aatcaccagc
     9061 gatcccgctt cgccctgaat ctcgcttccc aggacagaat cactgacctt tgagtgctgc
     9121 aaggtgacgc tgaaatcacc gtaatccatc accaccacgc cgtgggcgtc aacgccgctt
     9181 gccagcaaac tcgccgttgc ctgcacgctt ttcggctcac caaataacgc caccgccgac
     9241 gccaggcagt aaaagccgat atccataatt gaaccgttag agaatgccgg attaaaggtg
     9301 ttgggattct caccgtccag gtaacgttga taccgcgagg aatactggca atagttgaaa
     9361 aagactttac gcagtttgcc tgctttcggc agcgcctggc gcaacaaatg aaagttcggc
     9421 aggcaggcgg ttttaaatgc ctcaaacagc accacctgat tttcgcgcgc acaggcaatg
     9481 gcggcatcca cttccgccag attcgacgcc agcggttttt cgcaaatcac atgaatttta
     9541 tggctaagga aaagttgtgt ctgggaaaag tgcagggaat tcgggctggc aatatacacc
     9601 gcgtcaatgg catcgctttc cgccatcgct tccagcgacg taaacagatg ctcgacagaa
     9661 aaatcattgg cgaagtgctg agcctgctca aggctgcggg aatatacggc ggttaacttg
     9721 tatttaccgc tctcatgggc ggcctcgacg aactggcgag tgatccagtt cgtaccaatc
     9781 acagcgaaac gtatcataac gcgtggatac tccataaagg gattctgtcg tcaatgtagc
     9841 acgccttttt catcttcgac gtgcgcagat acgcattatt ctttcgccgg acagccatgc
     9901 tcactaaacg gcccaatcat gaccgatggc ccgagatatt tcggctggcg atggaggatt
     9961 tttgcgtcaa tcaccgcgct gacacgagcg agtttctccc gcagttgagt ataaaaaccg
    10021 tgcttcatat tgaggtctgg cgcgttaaac ccgatagcgt cgatgccgta ctgttttgcc
    10081 agccagatgg tgcgttggtt atgaaattct tgcgagataa tggtgatatg gttttcccca
    10141 aacacctttt tggcacgcac taccgaatcc agcgttgaga atccggcata gtcgcaaaag
    10201 atcactttcg ccggtacgcc tttagcgatc aaagcctgct gcatacctga tgcttcatcg
    10261 taatttttac gtccgttatc gccactcacc agtagccatt tcacttttcc ggcgtggtac
    10321 aacgctgctg cggtatcaat acgtcgggta aagtagcgat tacccggcct cgcccccagc
    10381 aataaaccca cgttacgcgc cgggacggcg ttgacatcgc cccaggtcag ctgcttgctg
    10441 gcgttgacca taacgcgatc ggcgataaaa atggtcgctc ctgccacaag taacagtaag
    10501 caggcaatct ttagagtccg gcgagaaaaa caaatacgga gaagaaggcg ggcaaatgcg
    10561 cgcagcatgg tcgttatcct taacgaaagg ggggaggtcg attttaggcg ctaaagaaag
    10621 aaaagataac gcctgacatc attttcagat tttgtcctgc ccaagcgaca actgcgttag
    10681 ccacagccgg gtgtcgaact caagctggtg gtactgcggc tccatatggc agcacaattg
    10741 atagaacgct ttgttatgct ctttctcctt caggtgcgcc agctcgtgca ccacgatcat
    10801 gcgcaaaaac ggttccggcg cgttgcgaaa cacggtagcg acgcggatct ctgcttttgc
    10861 tttcagcttg ctgccctgca cacgagaaac agcggtatgt aacccgagcg cgttttttag
    10921 cacatggatc ttattgtcat acatcacttt attgatcggc ggggcattac gcagaaactg
    10981 atttttcaga tcctgagtat attgccagag agctttatcg gtggcgtagt cgtgtgttcc
    11041 cggatagcgt tttgccagca catcgcccag acgctgttcg ttaatcagtg ttcgcacctg
    11101 cgaaagcagc tgctcgggat aaccctgaag ataagtaaga ttgctcataa ccgcccacga
    11161 aaaagagaaa aagggtatac tcacgcaccc ttttcagggg caacgtcgaa attttatcat
    11221 tcaggagggc cgatgagcca cttagacaac ggtttccgtt cactgacact acaacgtttt
    11281 ccggcgacgg atgacgttaa cccgctacag gcgtgggaag cggcagatga atatttgctg
    11341 caacagttgg acgacacaga aatcctcggc ccggtgttga tcctgaatga tgcctttggt
    11401 gcgttaagct gcgcgctggc ggaacataag ccgtacagca ttggcgactc atacatcagt
    11461 gaactggcga cgcgcgagaa tttacgccta aacgggattg atgagtcgag cgtgaagttt
    11521 ctcgacagca ccgccgacta cccgcaacag ccgggtgtgg tgctgatcaa agtgccgaaa
    11581 acactggcat tgctggaaca gcaactgcgt gcgctgcgca aagtggtcac gtcggataca
    11641 cgtattattg ccggtgccaa agcccgtgac attcacactt ccacgctgga actgttcgaa
    11701 aaagtgctcg gcccgaccac caccacgctg gcatggaaaa aagcgcgcct gattaattgc
    11761 acgttcaatg aaccgccgct ggccgatgcg ccgcagaccg ttagctggaa gctggaaggt
    11821 actgactgga ctatccataa ccatgcgaat gtcttctccc gcaccgggct tgatatcggc
    11881 gcgcgcttct ttatgcaaca tctgccagag aatctcgaag gggagattgt cgatctcggt
    11941 tgcggtaatg gcgttattgg tctgacgctg cttgataaaa acccgcaggc gaaagtggtg
    12001 tttgtcgatg aatcgccgat ggcggttgct tccagccgtt tgaacgttga aaccaacatg
    12061 ccagaggcgt tggatcgcag cgagtttatg atcaacaacg cgctctccgg cgtggagcct
    12121 ttccgcttta atgctgtgct ctgcaaccct ccgtttcacc aacaacatgc gctgaccgat
    12181 aacgtcgcct gggagatgtt ccactacgcc cgccgctgcc tgaaaatcaa cggcgagctg
    12241 tatatcgttg ccaaccgcca tctggattac ttccataaac tgaagaaaat tttcggcaac
    12301 tgcaccacta ttgcgacgaa taataaattt gtggtgctga aagcggtgaa gctgggacgt
    12361 cgtcggtaag attgggagtt acctgccgga tgcggcgtaa acgccttatc cggcctacgt
    12421 tcagcacgtt acccccaggc cggataagac gcggcaagcg tcgcatccgg caatgtctgc
    12481 tcagttctgg aatcgcgctt ccataaaaca aaaaatccct gtcaccatcc tctacattct
    12541 cttgtttagc gtttttctac gtttattctt ccgtcacaca gataaattca tccgttgcca
    12601 atctgtcaac ggatgttatc atattgccaa tttgtcaacg gacgtgatga agaatgcacc
    12661 tgataactca aaaagcattg aaagatgctg cggaaaaata cccgcaacat aaaacggagt
    12721 tggtggctct ggggaacacg attgctaagg gatatttcaa aaaacctgag tcattaaaag
    12781 cagtattccc atctctggat aacttcaaat atctggataa gcattatgtt ttcaatgttg
    12841 ggggcaatga attacgtgtt gtagcaatgg tcttttttga atcgcaaaag tgctacatac
    12901 gtgaagttat gacgcataaa gaatacgatt tctttaccgc tgttcatcgt actaagggga
    12961 aaaaatgatt gcgattgccg acatcttgca agcaggagaa aagctaactg ctgtggcacc
    13021 ttttctggcg ggtattcaga acgaggaaca atacacccag gcgctggaac tggtagatca
    13081 tctgctgctc aacgatcctg aaaacccctt gctggatctg gtgtgtgcca aaataaccgc
    13141 gtgggaagaa tcagcgcccg aatttgcgga atttaatgcc atggctcaag ccatgcctgg
    13201 cggtatagcc gtgattcgta cccttatgga tcaatatggt ttaacccttt ccgatctgcc
    13261 ggaaattggc agtaaatcta tggtgtcacg cgttttgagc gggaagagga aattaacgct
    13321 ggaacacgct aaaaaattgg caacgcgatt cggcatttct cccgccttgt ttattgatta
    13381 acgcgacgtg ccgaatgcgt tgcatccggc gcttcaaata acgatttaaa tctccagcgc
    13441 cagccgtgtt ccctgggcaa ttgcccgtcg tgcgtccagc tccatagcca catcgcagcc
    13501 gccaattaaa tgcacagttt tcccgctatc aatcagcggt tgcgccagcg cgcggtttgg
    13561 ctcttgccct gcgcagatca ccacattgtc cactgctaat acctgcgttt cgccgttgat
    13621 caccacatgc agcccgtcat cgtcaatctt ctgataactt acgcctggga tcattttcac
    13681 accccgcgag agcagggtgg tgcgatggat ccagccggtg gttttgccta acccctgtcc
    13741 cggtttgctg gctttgcgct ggagcatcac aatttgccgt gggctacgcg ggatctgcat
    13801 tccctgcggg cttaagccac cagcctgttg taggctgctg tcgatccccc actcattaca
    13861 gaacctggcg atattctggc tggtggattc gcccggctga cttaaataca tcgccgtatc
    13921 aaaaccaatc ccgccacaac cgatgatggc aaccttgttg cctaccggcg ctttgtcgcg
    13981 cagtacatcg agataactca ataccttcgg atgatcgatc ccgtcgatag gcggaacacg
    14041 cggcacgatc ccactggcga ggatcgtttc atcgaacgcc tgtaactgat ccgccgtcac
    14101 ggtgtgattg agtttcagcg tcacgcccgt cacttcgatc atccggcggt agtagcgcag
    14161 cgtttcgtaa aactcctctt tgccggggat ctgtttggcg atattaaact gcccgccgat
    14221 ctcgctgtgc gcatcaaaca atgttacctg atgcccacgc gccgccgcgt taatggcaaa
    14281 cgccagccca gcaggtccag cgccgaccac cgccagattt tttttctgca cggcgggaag
    14341 gattggcatt ttggtttcgt ggcaggcgcg aggattcacc aggcacgagg tgactttgcc
    14401 aacgaagatc tgatcgaggc agccctggtt gcagccaata caagtgttga tctcatcggc
    14461 tcgccctgat tgcgcttttg acagcagttc cgcatccgcg agaaacggtc gcgccatcga
    14521 tactatatcg gcatcgccgc gcgagagaat atcgtcggca acctgcggat cgttaatccg
    14581 gttggtggtt accagcggca gcgagacgtg gcctttcagt ttgcgcgtga cccagctaaa
    14641 tgcgccgcgc ggcacgggcg tggcgatggt cggaatccgc gcttcgtgcc agcctattcc
    14701 ggtgttgata atggtcgcgc ccgccgcttc aatggcctgc gccagttcta ccgtttcggc
    14761 aaaagtgccg ccgccttcga ctaggtcgag catcgacaac cggtaaataa taatgaagtc
    14821 gttgccgacg cgttcgcgca ccgtacgcac tacttctacg gcaaatcgca tccggttgcg
    14881 gtaatcgccg ccccactggt cactacgctg attggtgcgc agcgtcagaa attcgttgat
    14941 caaataccct tcagagccca tcacttcgac gccatcgtat ccggcctccc gcgccagttg
    15001 cgcgcagcgg gcgaaatcgt cgatcagttg caggatctct tcatgggtga gttcgtgagg
    15061 aacgaaacgg ttgatggggg cctgcaatgc ggacggtgcg accagatgcg gttggtagct
    15121 gtagcgcccg gtatgcaaaa tttgcagggc gattttgccg ccttcctgat gtacggcttc
    15181 ggtaatggtg cgatggtgtg ggatctggct ggcgtcgttg agcattgcgc caccttccat
    15241 gccaacgcct gttaaatctg gtgcaatgcc gccgctgaca atcagcgcca cgccgtgacg
    15301 ggcgcgttcg gcataaaacg cagccagccg ttcagcaccg tccgggtatt cctccagccc
    15361 ggtgtgcatt gaacccatca acacgcggtt ttttaacgtg gtaaaaccta aatccagcgg
    15421 ggcgaacagc gacgggtagc tcataaaatt gtccagtatg taaaataatt gttatgtagt
    15481 cggatgagtt ctaatttagc cgtcgatgga gaaaagggaa aaggggagtg cggggtttgt
    15541 gatgggattc aaaaagaata cgcccggtgg taacgccgga tgcgacgctt gccgtgtttt
    15601 atccggcttg ggggattgca catgtagggc ggataaggcg ttcacgccgc atccgccagc
    15661 cgttgcaaat tactgcttac ggaaaaaatc gttatacaac atatacaaat gagcggcact
    15721 ccaggagaaa tttggtgcgc cttgctgcgc gcctgtcagc gggttgtaat tctcctgaat
    15781 tgggccatcg gcggttaatc ctttggcgtg cctgaagaac gtatccgcca gcttcagggc
    15841 atcatcacga taaccgtaac gctccatccc tttcagacca aaccagaact gatccaccca
    15901 tacgcgcccg cgccagtaga tatcagcgcc aaaagccgga ttggttaacg ccgccgttcc
    15961 cagcggaaca aatgtgttga actctttagg atcgagcatc accttcacca ccgcgtcggc
    16021 attggcctgc gttgccgcac cgttaaacag cggcgaccag ccttccggcc ctttaccgcg
    16081 ctcaacaatc ggtttgcccg cgcagccgtt cgccagtggt ttatcttcaa tacgtacgtc
    16141 atagtagaac tgggtagtcg ggtcgaacat acaggtgttg atgtagtccg cgagctgctg
    16201 tgccaactgg cgatagcgtt tggcctcttc tggcttaccg aggatggttg ccatctccgc
    16261 cagataatgg ttatcgctgt acatatagct ggcctgatcc accgactcct gcaatagcga
    16321 gtagcccagc aacgttccgt cctgactgcg gttttcggcg aatttcaccg tccagtcgct
    16381 acgtttgccg ccattagcga catatttatc caactgttct ttgtcgataa aaccaaagac
    16441 ggcggcatca tcacgccccg attcccacga cgcagcgacc tgtgccggaa tttccagact
    16501 gtcgtactgg cctttctcca ccacgcgggc gtagttgttc agcccagact gcgtctcttc
    16561 tttatcgcct tttttcaccg taaacagcat ctcgccgctc tcggtgttgt gggctttgtc
    16621 gcgggtcgcg ccatattccg gcaccccgtt gccgttatga tcgcggttac gtaaccacca
    16681 gtcgtgatag gccaccagtt tcgggtacat ctctgccaac caggctttat cctgggtgac
    16741 gttgtatact tccatcaccg accaggcggc aaggctgggt ttggtattac gttcgttcca
    16801 gttgccgcca tcgccaccac gctcggggct aaggttccac gctatcaggt cggggacaaa
    16861 gcccacatcc tgcggacgca cgctgtcgcc aggctggatc tgccaggaga agaccgcgcg
    16921 gatattctct ttggcgatat ccggattgaa atgcgccatc gcaaacgcct gcttccaggt
    16981 atcccacggc caggtctgat tgccggagaa ccagcgtccg gtcaccgacg gcgtgacggt
    17041 gttataattc accgcaccgc ccggtgagcg ccagttaccg ttgagcgttt cgatcgcttt
    17101 caccgcgacg cgcgtctgtt ccggcgtcgc atccggattg gttaaccctt tcttcaggta
    17161 ttcttcccag cgttgctgcg aggcggtgag ataaaacgcc ggacgcgcca gaatatcgcg
    17221 gatctgcatt tgctctttgc taacttcctg agcggtcagc agatgggaat aggtggtgta
    17281 gagcgtggtc gaaccgttga tatgcgcctt actggtaaag cgattaccgt tgatttcagt
    17341 ctgcaccggc agggatttat gcacctgata ttccgattcg ccggaggtca gcagatccca
    17401 ggtggcgcgc actttgccaa aggtgacttt caggccatca cgggtggcgc tgattttgcg
    17461 ctgatagtca gggtattcgc cagcaatggt tttatcggaa agcggtttcc cttctttcgc
    17521 ttccagtttt tccagcagtt cgccatccca caccagatcc agcggtttat tgctgatgat
    17581 tttagtttcc agtagtgacg tgcgcggcgt ggcgaagcgc agagtcattt cgacctgcac
    17641 atcttttgct gtcagttttt gcaccagcgc gccgggaata ctgtatgcct ccagcgtgaa
    17701 gttgactttc ttgccgtctt gccagacagt caggcggtcg aaattgctgg ccataaagtt
    17761 gatgtattct tccgtcagca gcgcaacgcc cggaaagccg cccatggtgt tcgggccgtc
    17821 tggcaacaga tgaccatgcc aggctccgag atcgaaaaac ggattaaaac gctggtgatc
    17881 gtcgtaatcg taatccttca tgtactgcgg cgcaccagta cggttaatca cgtttttata
    17941 attgtcggcg ttagcggcat gagcggaaaa acttatcagc agagcgcagg ttactggcgt
    18001 taaaatagtt ttgattttca tgagattaca gtccttatta ccagtagaaa tattgcatca
    18061 ggaagatttg gtcgctggga tcgttagagg catttttcat gtagaagcgg ctatcaaaag
    18121 ccgtggcgaa acgtccgccg ccatattcgt accagatacc aaactgcatg aaagaggtgg
    18181 tttcatcgtc agcgtggtca gggcgatgtt tggcgtaggt gtaagcggtg gagagataaa
    18241 cgccttttaa cgcttcatac atggcgctca ccatcatccc gctttcactt cccggtacgt
    18301 ccagcccgtc ggcacgtccg gtatggtgcc aggcgcgagc ggcaaagtta ggggtaaaca
    18361 ggcccgtgag atagagttgt cctgtgccgt cggtaatttc cagaccattc atccaggtga
    18421 tgccttcggc aaggtcatac tggatgtaac cgttcatcca ttgccggata ggtgtatttg
    18481 tcgtcgtagc gatcgtactt gccaaaatgt agccaggctt tgctttcgtc atgatgtccg
    18541 gcaggtgtgg cggtgacgct ataacgaaag ttgccgctta agttctgtac cttcagcgcg
    18601 gtcataatgt cgcgggtatt gggaatgacg tagcccaaat cgggtgtgaa gtcgccccac
    18661 cattgcaggt catccagcga ggaatcctga cgcagactga gcatgatttc ggtgccatca
    18721 ttggtacgat aaccgccata gaaacggtta atcccacctt cgaaaccacc ccagctatgg
    18781 tcaggcacct aggcattacc gtcgtggtcg gcctgcacgg tccagccttc gccgtaaatc
    18841 aggccgaacc aggagccgta cttcatctcc aggccacctt caatataggt gccgtcgctg
    18901 tatttatgtt tattctcacc ctctaaatcc atatatccac caagccctaa ttcgccgtaa
    18961 aaacgaaatg aaggagaaga tgtcgaggta attgcgggtg tttttgtgtc gtcatgttct
    19021 gccggacgtg cttcttctcc tgccgcagaa aaggcgtagc aaaacggcag agcaagtaat
    19081 gctctgcatg gtgcagtgat taatttcatt gtgttaatcc atgagctaaa gaaaaagtac
    19141 agccttttat cgttgtaatt ataaaaatat aaaggcagaa actattttcc ctctcctcat
    19201 ccaggaagag ggaaaatgcc tgctcatttt ttattagtct gcttaatcaa cattgctggc
    19261 tggcgtcgct tcaatatggc gctcttcagc cgttaatccc tttatatatt tgctgtattt
    19321 ccaccacgca aagccgagga agataacaat accgccgacg ttataaaaaa tgatggtcag
    19381 aatattcgcg ccagtcggga atgtcgaagc gacaaaccct acggcaaata tcgcaatcag
    19441 cattgaaaca acgataatac cggtgcggcg ggagcccata cggaaatcgc gtggcaggtg
    19501 atctaatttg gcgcgtaaat tcaggtaagc cagcatgatg aataacggcg gaagcatgga
    19561 cgctgcggcg gtcatattaa taatggtatt catcagatcc tgcacggtat tggaaccgag
    19621 catcgggata atcatcagcg ggatgacgat cagaaactgg atccatgctg cgcgcgccgg
    19681 aacgccgttt tcgttaagtt cgacggtttt cttaccaaag atgccttccg ggatttcgga
    19741 gaagaaaatt ttcaccggcg ttgcggtcca catcagcagg gaaccgaaca ttgcggtaaa
    19801 ggacaccaga ccgacaaagc gattcatcaa cgcttccggc agaccaaaat acgccgccat
    19861 gccgtggaat acctgcaccg atccgccggt aaatttcaac tctttgctgc tgacgaagac
    19921 gttaatcagc accgaggaga cggaatacag tacgccgata aaaatcccgg cgaggatgat
    19981 cactttaacg aacgatttcg aaccgccttt gacgtcgtta acgtacaccg cgacggactc
    20041 cgcgccacct gcggccataa agatccaggt ggtaacgccg aggaacgccc agttgaagtt
    20101 cgggatcatc gcgtcaacgg tgatcggatc ggcaggctgt acgccgccaa ccagtgccgt
    20161 acctgccagt aaaatgtagg agagcgttaa cagcagcatc agcgttgaag tgacggaggt
    20221 aattggcccc agcattttcg ccccgttggt ggaaacccag gtggagaagg cgaacagcac
    20281 catactgata acggtggtgg caaccggcgt cataatatat tcgtagccga ggaaggcata
    20341 cgaagcataa gcaataacgc gcggcaacag cgaggtgaaa aagaacagat ttacgaacca
    20401 gtaagtgtag gcagtaataa atgcccaacg tccgcccagc gaacttttta cccacgcgta
    20461 gacaccggct tctgagtttt tatttaacga aacaaattct gcgatgatca gacagaaggg
    20521 aataaaataa aaaatcgtcg cgaggaaaaa catcggtgcc gaggccagtc caagctcaat
    20581 attattattg ataacgttgt taaagctgta aacggcggca aaagtcagcg agagcaagcc
    20641 gaatttgccg attgtattac gtttggtatc agacataaca catctccgaa aatgtgtagg
    20701 gtagcggtgg cggcaaactc cttttgccgc cgtagttgtt atttgttatg gaaataacca
    20761 tcttcgatgg tgactttgag aaccactttt ttgaccgcgt tattgcagat aaaccgatac
    20821 gcttcatgga tatcgcaaat aacgatttgc ccttcgtgga cctctacggt ttctccgcag
    20881 ccttttaaat attcacggtc agtttcatca cgataatatt ccactacctg taatgtctct
    20941 ttcggcgcat attcaatttt ttgctgccct tgcaggtagt aatgcacttc aaaatagcgt
    21001 cgatgcccgg taaatagcgc atcggtcgcg gagtcatttt ccacacggta ggtcaatgaa
    21061 tcaccgatgg agtgggcgac gccaggctga atgttgtcga tattttcaat cgcttcaacg
    21121 cagcgttgcc acttcttgcc agaggcgtaa atctggcgga actgttctaa gttatcgatg
    21181 atcctcattg cttattctcg ctgtgcaaat tcgtggaaaa gaaccctgcg ccgaactcat
    21241 acgacgccag gctttgcgcg gtagcttctc cgccagaaac cggcagcaac gtaaagccgt
    21301 agctgaagtc acggaaccag acgcgccagg agtccagcac ctcgctgccc caggagttgg
    21361 agccgaggcc aagtagctgg tggtcgaggt tcagggtgat gtcatcactg cgctgcagct
    21421 cgttacagtg ctgggcagcg tggatgtttt cctgggtata gtgccaggcg ctgaagttaa
    21481 ttgggcgctg cggaaccacc agcagaccgt tgccgtggcg gttagtcagt gccgtccagc
    21541 ggacatgctg acggttgccg ttgttctgcg ggaagggata gttctcgaac atggcatcga
    21601 cggtgctgcg ccagatatcg atgatgttag cctgctggct gtcggcgtag ttttcgcccg
    21661 gtccacgacc gtaatacgcc acctgatcgt attcgccgtt aatccccatg gtgaaaccga
    21721 tgcacggaat gatgtgcgga tagtcgccgt aacgctcgcc ggaaagcgcc acgttaacct
    21781 ggccatcggc agcgatgcgc cagatgtagg tacagcgcat cccgaagtca aacaccggcg
    21841 gtgcaataac tgtgcggctg atgatcaaca cttcaccatc gctctgttct acggcaaagt
    21901 cgcgcagatg ttcctgcatg atctgcaaat gattcggctg ccacaaccct tcgtactcct
    21961 gcttgtggtt gtcgatcatc ggcttgaaga agttgatctt tggctcgcgg gtcagcagcg
    22021 actcgccatt tacctgccag gatgtcggtt tgccactcat ttttgagaag gtgatcgcga
    22081 agttgtagcc gcgaacggtg cagctcaaac gatcgtcttc cagcgtcagc ggacgcgcat
    22141 tatttggtgc gaaaggcact ggctgcgcgg tgttttcctt cagcgggaac tgataagtgg
    22201 cgatagaatg cccggcttcg ctgtagcggg tgcgggaatc tttggtcacc gtaatgttga
    22261 ggaacgcttc gcgggcgtcc agctgcggca gcgtgatctg caagggggct tcgctgttcg
    22321 gcgcaacgtc gcgcagttta atctgctgcg tcgcgagcgt ttcaccttcg gcgcgcacct
    22381 ctgcgtgcag ggtgtagtca tcaagcgtgg taaaccacag tttattttcg actttcaact
    22441 cgccgcgagt cagcgcgtgg atttttaccg gcgcgataac ctgtttgtac tctttcaggc
    22501 ccgatcccgg cgtctgatcg gaatagatca aaccatcaag acagaagtta tagttgttgg
    22561 gatagtcgcc gtagtcgccg ccgaatttat accagacatt gccgtggtcg tcctgtgcct
    22621 ggatcccgtg gtcgcaccac tcccagacat aatgaccctg aatgcaatcg tgcttataga
    22681 agacgttctg gtactccgtc agcccgcccg gtccgttccc catcgcatga gcatattcac
    22741 agatgatgcg cggcttcgga tgcgggtatt caccaaactc attcatcagc ggcacgcggg
    22801 tgtacatggt ggaaataata tcgaccactt cagcatcgcg atcttcttcg taatgaacca
    22861 gtcgcgtgtc atccagcgct ttcgccgcat ggtacatcgc gcggatgtta cagccatagc
    22921 cggattcatt gcccagcgac cagatgatga tcgacggatg gtttttctgt gcgtggatat
    22981 ggcgaacaat gcgctcgacg tagacttttt cccactgcgg atcgtcggta atacggctga
    23041 tatcgccgac attagcaaag ccgtgcgatt cgacgtcggt ttccgccatc acaaacaggc
    23101 cgtagatatc acacagttcg taaaaacgcg gatcgttcgg gtagtgagcg gtacgcacgg
    23161 agttgatatt gtgctgcttc atcaactgga gatctttctc gacgcgatcc attccaacgg
    23221 cgcggccttt gcgatgatcg ttgtcgtgac ggttgacgcc gtgcagcatc acataacggt
    23281 tattgatcca gaacagaccg tcgcgcactt tgatatcacg gaagccaacg cgttgtggca
    23341 ccacttccag aacgttgccg ttggcgtctt tcagcgtcat gaccagatgg taaagataag
    23401 gggattctgc tgaccattgc tgcggctgtt cgacagtaaa agcaaagctg gcgctggtca
    23461 gtttttcaat tgccaaatga tcaatggcgc tgctgtgcac cacgcgttcg ccatcaaaca
    23521 gggtatattc cagcgtcgtg acgacagggg aggcggcgag attttccagc accacttcgc
    23581 aggaaagcgt ggcatcgcaa taggcttcgt caaagtcggt acgcacagtg aaatcgttaa
    23641 tatgcgttag gtgttttccg accagataaa catcgcggaa gatccccgcc gaccaccaca
    23701 tatcctggtc ttccacgtag gtagagtccg cccactgcat cacgcgcaca cacaacaggt
    23761 tgtcgccggt tttaaccatc gcgctgatgt caaactctgc ggtcaggcga ctgcccttgc
    23821 tgaaacccac atactgaccg ttaacgtaga cttcaaaata ggtttcgacg ccgtcaaatt
    23881 taatcagcgt ctgtttaccc tgccagccgt cgctgagggt gaaaatacgt tgataggcac
    23941 cggttgggtt atcgctgggg acaaacggca catcgatggg gaacggaaaa ccttcgtcgg
    24001 tatattgcag tttgccgtga ccttccattt gccacatggc ggggacggta atatgccccc
    24061 agtcagccat taactcagag gtgaaggctt ctggtacttg cagcggatgg tcaaaaaagt
    24121 ggaaattcca ctgactgctt aagggcagaa acaggctgct ggtttcgcgg gcaaaggtac
    24181 gcgcttgcgc aacagaatca tatgaaaaaa agtacgcacg cggcgcaagt cggttttcgt
    24241 gggtgagctg aatgttttcc cagcgattca tagggcctcc cggtagagag aaatcggaac
    24301 cattcatggt gatggtagtt tgtgtaattg ctgggggttt agtaaaattt ttactaaaaa
    24361 gcagcaggta aatgcattta ctttaactgg atcacgaaaa taaacggcag cactgtccgg
    24421 caaaaatgat tgccgaacag tgtgtaaggg gatttaacgg gtcgtgccgc gcagttttaa
    24481 tttgctggga acgaagacta gcagcggcag cgcgcgacca tcgcgggctt tttcatacac
    24541 caggttaacg ccctgacttc ccatcatctc ggaatggatg cgcacggtgg agagcggcgg
    24601 aaaggtaaat cgcgcggtgg ggatatcgtt aacgctgata agcgaaatat cttgtgggat
    24661 gttcaggcca cgttcatgaa ttgcccgcag tacgccgata gcaatggaat cggaagcaac
    24721 aaacagtgcc ttcggatagt cttcccgcgc cagcatttgt tttgccagtt cataacccga
    24781 cgaactggaa aaaccgccgc gccagatatc ctcttcgcgt accacttgtt tcagtcggcc
    24841 atattccgca aaggcgacct cacgaatatc cgccttgcca ggctcatctt caccgccaat
    24901 aaaaccaata cgattaacgc cctggttgat atagaagtcg atgatttctt tactgatgcg
    24961 tgccagatcg atatccaccg catcgtaacc gctgccgggt tcgtgaaagt cgataaaaca
    25021 gatattgtcg gtcaacgcgc tggcagcggc gcgcagggcg ggcgtgggtt tgccgacaat
    25081 taaaataccg gtgacgtttt taatgtctgg taatccgttg tgttcataac agttggtcag
    25141 ttcgatcgcc agcttttcgc actgagtttc aatgccgtgg cggatcgcca gatagtaagg
    25201 atcgttgatc tccagctcct gctggtagct gtagatagcc agaatatggt gttggttgac
    25261 tgcgcctgtc tgtagtttac gggcgctact ggtcttgtac tccagctttt cggcgatctc
    25321 gagaatgcga tgtttcgtct cttctttcac attcaatgtc ggatcgtcat ttaagaccct
    25381 ggatactgtc gccagggata cgccagcttc gattgcgatg tcttttaatg ttgccatgtt
    25441 tacctttttt actttccctg atgtgcgccc ccattgtaga ggaagtgctt atcgggtcac
    25501 ctgttttcag taaataaagc gcgtttcttt gacgctgtaa cggtaacaca gcggcttagg
    25561 aatgtgatca cgcctgcatt ctccattttt agcctacagg attgagaaaa acttttctat
    25621 agtttagtaa aattttactc gaagggagga agcaatggaa accgtggctt acgctgattt
    25681 tgcacgtctg gaaatgcgcg tcggaaagat tgtggaagtg aaacgccatg aaaacgccga
    25741 caagctgtac atcgtacagg ttgatgtggg gaaaaaacac tgcaaaccgt gaccagcctg
    25801 gtgccgtact acagcgaaga ggagctgatg gggaaaacgg tggtggtctt atgcaatctg
    25861 caaaaagcga aaatgcgtgg tgaaacgtcg gaatgcatgc tgttgtgtgc ggaaaccgat
    25921 gacggcagtg aaagcgtgtt gttaacgccg gagcggatga tgcctgcggg cgtacgcgtc
    25981 gtataaaaag atcggatggc gacgtcgtat cgccatccga tttgatatta cgcttcttcg
    26041 acacttactc gcatggccgc cagcgcctta cgcgccgctt tgatcaccag ttcacactgt
    26101 tcaatggtca gtgtcagtgg cggttcaatg cggatcgttt tggcgttatt gagcgttccg
    26161 gccaccagta cgcgctggcg gaacatctcg ctggcaaagt tatagccgat ttcgttatca
    26221 acaaactcaa tcgccatcaa catcccttta ccacgcgctt cctgtaccag atcgggatat
    26281 tcccgcgcca gttgacggaa accgtccagc aacatatcgc ctttttgctc agcctgagcc
    26341 ggtaagttct gctccagcaa cacattgatg gtcgccagcg ccgccgcaca ggccagcggg
    26401 ttgccgccaa aggtggtggt atgcaggaat gggttgtcga acagaactga aaacacctct
    26461 tcagtggcga tggtcgcgcc aatcggcatc acgccgccgc cgagggcttt tgccagacac
    26521 agaatgtccg gctgaacgtt ctcatgttca caggcaaaca tcttgcccgt gcgccccatg
    26581 cccgtttgta cttcatcgag gatcatcagt gcgccgaact catcgcacag cttacgcacg
    26641 gcggtgagat agcccggcgg cggcagaatt acgccacctt cgccctgaat cggttcgagg
    26701 atcaccgcag ccacatcatc accggttttt ttgcactcat taagagccgt gcgcatggct
    26761 tcgatattgc caaacggcac atgacggaac cctggcagca gtggcataaa cggtttgcgg
    26821 aaggtcgatt tcgccgtggc cgacagcgcg ccaagtgatt taccgtggaa cacgccgctg
    26881 gtggcaataa aagtaaattt gccgcgcggc gactgataag ccttcgccag cttcagcgcc
    26941 gcttcaacgg actcggtgcc gctattacag aagaagctgt atttcagttt accgggcgtt
    27001 agcgcagcaa gggttttcgc cagcatagcc cgtaacggat cgagtagctc ctggctgtgc
    27061 agtggttgtt tcgcaagttg attctgtacg gcggaaacca caactggatt acggtgcccc
    27121 acgttgaaaa ttccaaaacc tcccaggcag tcgataaact cctgtccctg ggtgtcgaca
    27181 agcgtattta aacctcccgc ttgccactct acggctccgt aatccccgcc ggcggtaaca
    27241 gatttgcgat actctaaaaa ccccggattg acatgctctt tgaagtattc aatcacctct
    27301 cggttaagtg ctttcatctc ctcatgatcc agcgttcgct tctcaatgag attcagggcg
    27361 tgggcgctgc acgctaaagc cgatgcgctc gaaggtaacc tgttcaaaat atgctccgga
    27421 ggctcgcgta tcacatgata ccgatttaag tattgcaggg attgcgccac tccggctgcg
    27481 atcgcgaaaa tcgggataaa cacaaatgcg gttatcattt gtgcaatatt taatgctatc
    27541 cgttaacatt tgttgttatt ttgcacggtt tttgtcgggt atggatttga tgattgctgc
    27601 gcaaatgcac gttttcaggg caataattgc ctgatttttg ggcaagataa ttaacgtttt
    27661 cgtatagtaa cagagtgaaa gtattttaaa tcaaagaatt agatttgtgc tttaatcgca
    27721 aattgcgatc taaatcaaat taatcggtta aagataaccg cagcggggcc gacataaact
    27781 ctgacaagaa gttaacaacc atataacctg cacaggacgc gaacatgtct tctcatccgt
    27841 atgtcaccca gcaaaatacc ccgctggcgg acgataccac tctgatgtcc actaccgatc
    27901 tgcaaagcta tatcactcat gctaatgaca cttttgtgca ggtgagcggc tttaccttgc
    27961 aagagttaca agggcagccg cacaacatgg tgcgtcaccc ggatatgcca aaagcggcgt
    28021 ttgcggatat gtggttcacc ctgaaaaaag gggagccctg gagcggcatc gtgaaaaatc
    28081 gccgcaaaaa tggcgaccat tattgggtgc gggccaatgc ggtaccgatg gtgcgcgagg
    28141 gaaaaatcag tggctatatg tcgattcgta cccgggcgac ggatgaagag attgcggcgg
    28201 tggagccgct gtacaaagcg ttgaactccg gacgtaccag taagcgtatt cataaaggcc
    28261 tggtggtgcg taaaggctgg ctgggtaaac tgccttcatt accgcttcgc tggcggacgc
    28321 gtggagtgat gaccctgatg tttatcttgc tggcggccat gctttggttt gttgctgccc
    28381 cggtggtgac gtatttcctc tgtgtgttag tggtattgtt ggcaagcgct tgttttgaat
    28441 ggcagattgt gcgcccgata gaaaatgtcg cccgtcaggc actgaaggtg gcgaccggag
    28501 agcgtaatag tgttgagcac ctgaatcgca gcgatgagct ggggctgaca ttacgcgcgg
    28561 tagggcagct tggcctgatg tgccgttggc taattaacga tgtctcaagc caggtgtcca
    28621 gtgtcagaaa tggcagtgag acgctggcga aaggcaccga tgaactgaac gaacataccc
    28681 agcagacagt tgataacgtt cagcaaacgg tggcgaccat gaaccaaatg gcggcgtcgg
    28741 tgaaacagaa ctctgccacg gcgtcggctg ccgataaact ttctatcacc gccagtaatg
    28801 cggcagtgca gggtggggag gcgatgacca cggtgatcaa gacaatggac gatatcgccg
    28861 acagtaccta gcgcattggc accattactt cgctgattaa cgatattgcg tttcagacca
    28921 atattctggc cctgaatgcg gcggtggaag cggcgcgtgc cggcgaacag ggcaaaggtt
    28981 ttgcagtggt ggcaggggaa gtgcgtcatt tagccagccg cagcgctaat gctgccaacg
    29041 atattcgcaa gctgattgat gccagtgctg ataaggtgca atccggttcg cagcaggtac
    29101 acgccgccgg acggacgatg gaagatattg tggcacaggt gaaaaacgtc acccagttga
    29161 tcgcccagat tagccattca acgctggaac aggccgatgg gctttccagc ctgacccgtg
    29221 cagtggatga gcttaacctg atcacccaga aaaatgccga gctggtggaa gagagtgcgc
    29281 aggtgtcggc gatggtgaaa caccgcgcca gccgactgga agacgcggtg acggtgctgc
    29341 attaatcggt atgccggatc cggcggttgg agcacaatgc ctgatgcgat gctggcgcat
    29401 cttatcaggc ctgcggggtg tgtagcgggt gtaggccgga taaggcgcgt caagcgtcgc
    29461 atccggcaat tgcaccgcgc cactggcgga tgcggcgatt aatccagttg cgtaatatca
    29521 aaagcggcgc ggtcgatcac cgcaatgatc ttttttattt gtgcatcact gatatcgctc
    29581 tggttgacgc gtaaatccag caccgcttta aaattatcca gcgcccgctt catttgcggg
    29641 ttctggcgca gcgccgcgcc aacgcaacgc gctttgatgc gttcttcaat catctccact
    29701 tgttcgcggt tttcttccag ccactgcgcg ccttgttcgg tcagcgcaat ctgcttctta
    29761 ccaccttcct cttcgcggat ggtaatcagc gactgctcct gcagaaaatc cagcgtcggg
    29821 tagatgacgc ccgggcttgg ggtgtaattc ccctgggtta gattctcaat cgctttaatc
    29881 aattcgtaac cgtggctgtc atcgcgcgag agaatatcca gaatcaccag acgcaattca
    29941 ccgtgaccaa agaaacgttg ccgacgaccg ccgccgcgac cgtggcgacc accgcaacat
    30001 tgaccgtgtt catgctggtg accgtgtccg cagtgctcgt gttctgactt ctcacctttg
    30061 cagcagccct catggcgtgg ctggccttca tgtttacaac acccttcgtg atgatggctc
    30121 atatcagcct ccttttgctt gattagatat atctaattta tatctgatgt gagcattact
    30181 gcaagtacaa aaatgatttt taaacgtgga agcacctcac atctaggccg gataagacgt
    30241 tcacgccgca tccggcagca ttgctatggc actgaatgcc ttcacatact tcagacatat
    30301 cagttctaca aatcgcttgc atttatcatg attaacaatc attatcattt gcgaatttta
    30361 tttagatata tctgattacc atcacgaagg cgataacaat gaataacacc ccccgctacc
    30421 cgcagcgcgt tcgcaatgat ctgcgcttcc gtgaactgac tgtgctacgc gttgagcgca
    30481 tcagcgccgg ttttcagcgc attgtccttg gcggcgaggc gttggacggt tttacatcgc
    30541 gcggctttga cgatcacagc aaactcttct ttcctcaacc tgacgctcac tttgtgccgc
    30601 caacggtaac ggaagagagc atcgtctggc cggaaggacc acgcccaccg tcgcgtgact
    30661 atacgccact gtatgacgaa ctacgccatg aactggcgat cgatttcttt attcacgacg
    30721 gtggggtcgc cagcggctgg gcgataaact tacggtggca ggtccgcgcg gttcgctggt
    30781 ggtgccggaa gattacgcgt atcagctgta tgtctgcgat gaatccggaa tgcccgcact
    30841 gcgccgccgc ctggaaacgt tgagcaaact tgccgttaaa ccgcaagtta gtgcgctggt
    30901 tagcgtgcgg gataacgcct gtcaggatta tctcgcgcac cttgatggtt ttaatatcga
    30961 atggctggca catgatgagc aggcggtaga tgcgcgtctg gcgcagatgc aaatccctgc
    31021 cgatgattac ttcatctgga taaccggcga aggtaaagtc gttaagaatt taagccgccg
    31081 ctttgaagcg gaacagtatg acccacagcg ggtacgtgca gcggcttact ggcacgcaaa
    31141 gtaactgata tataaaaaga aaaaggctga cgatttctcg tcagcctttg ctatatctgg
    31201 tggcccctgc tggacttgaa ccagcgacca agcgattatg agtcgcctgc tctaaccact
    31261 gagctaaggg gccatggtag cggattataa agtaactccg tgccgcaatc cagccattac
    31321 cgcgcgcctg ctgtttttat aaacaatgta ttttcaatcc gttatacttt tcctggtgat
    31381 gttagaaagg agtaaatatg gttgaggata ttttggctcc agggttacgg gtcgtgtttt
    31441 gcggtatcaa ccctgggctt tcatccgccg ggactggttt tccctttgct catccggcaa
    31501 atcgcttctg gaaggtgata tatcaggccg ggtttaccga ccgtcagttg aagccgcagg
    31561 aggcacagca tctgctggat tatcgttgtg gcatcaccaa actggtagac cgtccaacgg
    31621 tgcaagccaa tgaagtttca aagcaggagc tgcacgcagg tgggcgtaag ctgattgaaa
    31681 aaattgaaga ttatcagccg caggcgttgg ctattctggg caaacaagca tatgaacagg
    31741 gattcagcta gcgcggtgca cagtggggga aacaaacgct caccattggt tcgacgcaga
    31801 tttgggtgct gccaaatccc agcggtttaa gtcgcgtttc actggagaaa ctggttgaag
    31861 cgtatcgcga gctggaccag gcgctggtag tgcgtgggcg ataa
//
LOCUS       DAZSNM010000016        31494 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZSNM010000016 DAZSNM010000000
VERSION     DAZSNM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31494
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.016"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..870)
                     /gene="rpnA"
                     /locus_tag="ACHW42_000712"
     CDS             complement(<1..870)
                     /gene="rpnA"
                     /locus_tag="ACHW42_000712"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFR8942656.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAA"
     gene            complement(1073..1309)
                     /gene="feoC"
                     /locus_tag="ACHW42_000713"
     CDS             complement(1073..1309)
                     /gene="feoC"
                     /locus_tag="ACHW42_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR8942657.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(1309..3630)
                     /gene="feoB"
                     /locus_tag="ACHW42_000714"
     CDS             complement(1309..3630)
                     /gene="feoB"
                     /locus_tag="ACHW42_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR8942658.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3647..3874)
                     /gene="feoA"
                     /locus_tag="ACHW42_000715"
     CDS             complement(3647..3874)
                     /gene="feoA"
                     /locus_tag="ACHW42_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR8942659.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4312..6633)
                     /locus_tag="ACHW42_000716"
     CDS             complement(4312..6633)
                     /locus_tag="ACHW42_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR8942660.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6730..7206)
                     /gene="greB"
                     /locus_tag="ACHW42_000717"
     CDS             complement(6730..7206)
                     /gene="greB"
                     /locus_tag="ACHW42_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR8942661.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            7434..8153
                     /gene="ompR"
                     /locus_tag="ACHW42_000718"
     CDS             7434..8153
                     /gene="ompR"
                     /locus_tag="ACHW42_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR8942662.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            8150..9502
                     /gene="envZ"
                     /locus_tag="ACHW42_000719"
     CDS             8150..9502
                     /gene="envZ"
                     /locus_tag="ACHW42_000719"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR8942663.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9578..11200)
                     /gene="pckA"
                     /locus_tag="ACHW42_000720"
     CDS             complement(9578..11200)
                     /gene="pckA"
                     /locus_tag="ACHW42_000720"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR8942664.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11579..13330
                     /locus_tag="ACHW42_000721"
     CDS             11579..13330
                     /locus_tag="ACHW42_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFR8942665.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            complement(13474..14343)
                     /gene="hslO"
                     /locus_tag="ACHW42_000722"
     CDS             complement(13474..14343)
                     /gene="hslO"
                     /locus_tag="ACHW42_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR8942666.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(14368..14769)
                     /gene="hslR"
                     /locus_tag="ACHW42_000723"
     CDS             complement(14368..14769)
                     /gene="hslR"
                     /locus_tag="ACHW42_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR8942667.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14780..15448)
                     /gene="yrfG"
                     /locus_tag="ACHW42_000724"
     CDS             complement(14780..15448)
                     /gene="yrfG"
                     /locus_tag="ACHW42_000724"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR8942668.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15513..17648)
                     /gene="igaA"
                     /locus_tag="ACHW42_000725"
     CDS             complement(15513..17648)
                     /gene="igaA"
                     /locus_tag="ACHW42_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR8942669.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            17968..18528
                     /gene="nudE"
                     /locus_tag="ACHW42_000726"
     CDS             17968..18528
                     /gene="nudE"
                     /locus_tag="ACHW42_000726"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR8942670.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18693..21245)
                     /gene="mrcA"
                     /locus_tag="ACHW42_000727"
     CDS             complement(18693..21245)
                     /gene="mrcA"
                     /locus_tag="ACHW42_000727"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR8942671.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21365..22144
                     /gene="hofM"
                     /locus_tag="ACHW42_000728"
     CDS             21365..22144
                     /gene="hofM"
                     /locus_tag="ACHW42_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR8942672.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            22144..22683
                     /gene="hofN"
                     /locus_tag="ACHW42_000729"
     CDS             22144..22683
                     /gene="hofN"
                     /locus_tag="ACHW42_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR8942673.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22667..23107
                     /gene="hofO"
                     /locus_tag="ACHW42_000730"
     CDS             22667..23107
                     /gene="hofO"
                     /locus_tag="ACHW42_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR8942674.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            23097..23503
                     /gene="hofP"
                     /locus_tag="ACHW42_000731"
                     /pseudo
     CDS             23097..23503
                     /gene="hofP"
                     /locus_tag="ACHW42_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            23415..24653
                     /gene="hofQ"
                     /locus_tag="ACHW42_000732"
     CDS             23415..24653
                     /gene="hofQ"
                     /locus_tag="ACHW42_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR8942675.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            25057..25578
                     /gene="aroK"
                     /locus_tag="ACHW42_000733"
     CDS             25057..25578
                     /gene="aroK"
                     /locus_tag="ACHW42_000733"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR8942676.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25635..26723
                     /gene="aroB"
                     /locus_tag="ACHW42_000734"
     CDS             25635..26723
                     /gene="aroB"
                     /locus_tag="ACHW42_000734"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR8942677.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26815..28107
                     /gene="damX"
                     /locus_tag="ACHW42_000735"
     CDS             26815..28107
                     /gene="damX"
                     /locus_tag="ACHW42_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR8942678.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            28214..29050
                     /gene="dam"
                     /locus_tag="ACHW42_000736"
     CDS             28214..29050
                     /gene="dam"
                     /locus_tag="ACHW42_000736"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR8942679.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            29068..29745
                     /gene="rpe"
                     /locus_tag="ACHW42_000737"
     CDS             29068..29745
                     /gene="rpe"
                     /locus_tag="ACHW42_000737"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR8942680.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29738..30496
                     /gene="gph"
                     /locus_tag="ACHW42_000738"
     CDS             29738..30496
                     /gene="gph"
                     /locus_tag="ACHW42_000738"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR8942681.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30489..31493
                     /gene="trpS"
                     /locus_tag="ACHW42_000739"
     CDS             30489..31493
                     /gene="trpS"
                     /locus_tag="ACHW42_000739"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR8942682.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
BASE COUNT         7356 a         8039 c         8528 g         7571 t
ORIGIN      
        1 cgccgcagcc aactcttctt ctgacagccc ggtaaatcgc atgatgtctg caagagggac
       61 tccggattca agcattattt tggctatatg cagggctttg gattgttccc cctcctgccg
      121 caatctttcc gcaatagtca ttaaactctc cttgtgtttc ggtgaacgtt cggcaacgcc
      181 gtcgataaaa tcgttaaaac gtacagcgtc gccagtttgc agtatgtaat taaacagccc
      241 tttgatttgt ctgtcattag cgtatccact acttaataag caggccattt gctctaccag
      301 ccccatcaga tcgcgttgac gaatatgttt ttgaattaac tccagcagcg ccatacgtcg
      361 gtgctgcatg atttcatcat caggcatgac ggtgacatca atcagcggaa atgcggaggc
      421 ataaagctgc cttgccagtt tgggatcggc gaaacaatcc agccaacaca gcgaataggg
      481 ataggggctt tcaataccgt ggtaaaacaa caatggcacc accatcggca acgttttgta
      541 tccagcatca aggtgatttt gcattgcggc aatagcgtaa cgcatcatgc gaaatgcgat
      601 cagtttgttt gaggtgcttt gatgttcaat cagacaatag atgtatcctg gtccttgttc
      661 cgttttcacc gaccacagca catcggaata gctttcacgc agatcgtcat caataaagct
      721 cctcgactcc agcttgaggg ttttcatatc acagagcgcg tgaatgggtg ccggtaaatg
      781 aaacgcaaga aaatcgcgtg ccgtctccgg ttggcgtaaa aagagtttga acagcgcatc
      841 atgtggggtg gaactctgct ttttactcat ggcgttcctt cacctgttgt ttgaatgccg
      901 cgactctacg cgccactcca catctgtgca ttagcgagtt ttcatccctg cgagacgctt
      961 tcagaggaac ttacgttgcg ctgcaagaat tgcattttta tggaaagccg cacgcaaaaa
     1021 agcccggatg caaacgcata ccgggctttt caaaacaggc gatggagtat ggttaacgca
     1081 gcgcccacca ctcgcgcaga caggctttgc cttccgggca gcttttacaa ctgccagaga
     1141 ggcagccgtc aggttcttcc tgaatccgca cggctttgcc catgctttcc agctgttgca
     1201 gcatggcgtt aatcattggc tgcggagtgt tcaatgtctg gcttatctgg gtcgcttcca
     1261 tacggccccg taacgccagc aaatcacgta cctgaataag tgaagccatt aatggcaatc
     1321 accggtggtg ctggctgcgc agcaactgct taccgacttg cgggttgcca gcagttcgat
     1381 atccacccgg ctacgcgcgc ggcgcagcag accgatagct acgatgttaa acaggattac
     1441 cgccagaatg cacaccaggc tgtaagttgg gtgctgactg tagctggcga cctgatagaa
     1501 caatgttgcc agtgagtaag cgatattcag cccccacagg atggagaagc ccatccagcc
     1561 acggcttgat tcacgggcga tggctcccat caccgagata catggtacat acagcaggac
     1621 gaaaatcagg tagctgtatg cggcagctgc gctaccgaat ttctgatcca tcacgcccat
     1681 cgccccggta cccatttcac cgttgccttt gctagcttca atggggttca tcagtacgct
     1741 aaggctgaag gtgtctttca ggctctgcca ggtttcatct accgcactga acagctcttc
     1801 accgaggttg aattccgccg gattgaactc ttcgtcctga atattttctg cggtgtagag
     1861 ggtgttgagc gtacccacca ccacttcttt tgccattgcg cccgtaaaca ggccaaccgt
     1921 tgcctgccag ttatcttcat gtacgccaat cggcttgaag acaggggtta tcacccggct
     1981 gacggaagcc agcgccgagt cgttgatgtt atcgacgatt ttcccgctca acgagaagct
     2041 gttgaaagcg ctcaggaaaa tgctgacgat gataatcact ttaccagcac gcagaacgaa
     2101 gcccttcaga cgctgccagg tctggataat caggctttta acgtgtggta catggtagac
     2161 cggcagctcc atgacaaacg gcgtcgcttc accgcgcatg atggtgtact tgagcatcag
     2221 gccagtcagc accgccatca cgataccgag catatacagc gagaaaaccg ccagggcacc
     2281 gttctgcccg aagaaggcag ccgcgaatac tgcgaagata gccagacgcg cgccgcagga
     2341 cataaacggt gccatcatga tggtcatcag acgttcacgc ggtgcatcaa gcgtacgtgc
     2401 gcccattacc gacggtacgt tacaaccgaa accgacgatc agcggcacaa aggatttccc
     2461 cggcaagccc agcgcctgca tcaggcggtc catcacaaac gctgcacgcg ccatatagcc
     2521 ggagtcctca aggaaggaga ggaacaggta catcatgccg atctgcggca ccagtggcag
     2581 cacggtgtta ataccgccac ccaggccctg ggcgaggaag atagtcagcc agtccgggaa
     2641 gtggagcgtg tagccaatcc attgaatacc atgcacaaat agcgccacgg agccgacgtt
     2701 aaacagcggc tgtaacgccc cgccgatgtt gatagccagc aggaacatca ggtacatcac
     2761 aaagaggaaa atcggcagac cgaggaaacg gttgagcacg attttatcta ccgcagtggt
     2821 gaaacggctg ggttctgccg tcagggtgtt gcttaccaca tcacagatgg cagcaatgca
     2881 ctggtaacgc gcatcggcaa tgtgcagcgc cggatcatcc atttcattac gcagacgggc
     2941 gagggtggca tccagatgct gcgacgcttc accggcgtag gcgcggctgt agatatcgcc
     3001 ttccagcatt tgcaggccca gccagcgacg ttgtttcagc gggatgtcgg aaggcatcac
     3061 ttttgccagt gaatctgctt cgttgagtag cggctgtgcg taatgcacca gttccacatt
     3121 ctcgttagct ttatagcgat caatcgccag cttgagcgct tcaataccgc gaccacgggt
     3181 tgaaaccagc gggatcaccg ggcagccaag acgtgccgat agagcatcaa tttcaatacg
     3241 aatattttgc ttctcggcaa tgtcgagcat gttcagcgcc acgatgcagg gaatgccgag
     3301 ttccagcagt tgcagcgtca ggtacaggtt acgctcaagg ttagacgcat ccaccacgtt
     3361 aatcagcagg tcggcgtcgc cactcaaaat gtagtgacag gcgatttgct catcgagcga
     3421 ggtctgcgat gagatggtgg tcagagaata ggtgccgggc aggtccacca gcgtgacctg
     3481 atgatcggtg gtggagaatt gcccttcttt acgttcgacg gtaacgccag cccagttacc
     3541 tacacgctga cgtgcgccag tgagctggtt aaataaggtt gtcttgccag aatttggatt
     3601 accaattaag ccaatggtta attttttcat tgttgttatc accgtattaa caggaaaccg
     3661 cttccacttc taataaggcc agatcttttt tgcgtaatac caggctcaca cgacgggttt
     3721 cgatatgaat ggggtcgccg agtggagcga cgcgcaccac atcaaaagag gagccaggta
     3781 acatgcctag agaaagcagt ttttggcgat atgccgggct gatttcacgg gaaaagccag
     3841 tgattttcca cgcagtatct ggagtgtatt gcataggtgc ctacttgttt ctcattaact
     3901 ggataactac ctcatggggc gtttgaccgc gacatgagac cagatgattt gccaacaaag
     3961 gagagattaa ataatcaaca acacaatgat aatgagaatg gtttctatca gcaatactta
     4021 aaatgtgtgc gaatgttggt ttgaataata atctgactca atgttgatgt ggctcaaggt
     4081 tatatcgcag cgaaaatata attcgaatag tgagttaccc ggactggcta atgtttaatt
     4141 aaggtttcag ttgttaatga agtgataaat attaacggag tatattgtga gtttggctgt
     4201 ggaataaaag tgccggagat attctccggc acttttatgt caggcaaagc gttcgttgcc
     4261 agatgcggcg tgaacgcctt atcgcgcagg tttaggggcc agtccgggcg tttaacgttt
     4321 tttacccatc gctgccgcca gcgcatccat catcgcgcta ttaccggcag gctgcgcttc
     4381 acgaccgcgt ggtttggctg ccgggcggtt gttttgcggg cgttcattac cgccgccgcg
     4441 acgagagttg gtttcgccag gctgctcgtc cagacgcata gtcagggcga tacgtttacg
     4501 ctgaagatcc acttccagca ctttcacctt cacaatgtcg cccgctttca ccacggtatg
     4561 cggatcttcc acaaacttgt tcgacaatga agagatgtga accaggccgt cctgatgcac
     4621 gccaatatcg acaaacgcgc caaagttggt gacgttggtc actgcgcctt cgaggatcat
     4681 acccggttgc aggtcgttca ttgtctcgac gccatcggca aactgagcgg ttttaaattc
     4741 cggacgcgga tcgcgacccg gtttttccag ctctttgatg atgtcagtta ctgtcggcac
     4801 accgaatttt tcatcagtaa agtcagacgc tttcaggtta cgcagttcgc tgctgttacc
     4861 catcagatct ttcagtgcct gctgtgttgc tgccagaatg cgttccacca ccggataggc
     4921 ttccggatga acggtagagg cgtccagcgg gttatcaccg tggttaatgc gcaggaagcc
     4981 cgcgcactgc tcgaaggctt tcggccccag acggctgact ttcagcagtt gctgacggtt
     5041 ctggaactgg ccgttctcat cgcgccaggc cacgatgttt tgcgccatca tgcgcgtcag
     5101 gcccgccacg cgggttaata gcggaacaga agcggtgttg agatcgacgc caacggcgtt
     5161 tacgcagtct tctactactg catccagttt gcgggccagt tgcgtctggc tgacgtcatg
     5221 ctgatactga cctacgccga tagatttcgg atcgattttc accagctccg ccagcggatc
     5281 ctgcaaacgg cgggcgatag ataccgcacc acgcagcgaa acgtcgagat ccgggaactc
     5341 ctgcgctgcc agctcggaag ctgagtaaac cgacgcgcca gcttcgctga cgatcacttt
     5401 ctgtgcggtc actttcggga actgcttctg cacgtcgaga tagaaacgtt cagtttcgcg
     5461 ggaagctgta ccgttgccga tcgccaccag ttcaacgtta tgtttttcac acaaggcagc
     5521 aacggtcatc gctgcttttg cggcttgtcc ggtgtgcggg taaatggtat cggtcgccac
     5581 cagtttgcca gtggcatcga ccaccgccac ttttaccccg gtacgcagac ccggatcgag
     5641 gcccatcgtt gcacgcagtc cggcaggggc cgccatcagc agatcgtgca ggttacgggc
     5701 aaagacgttg attgcttcat cttccgcgcg ttcgcgcacg gtacccatca gttcggtttc
     5761 cagatgcatc agcaccttga tgcgccaggt ccagctcact acgcttttgc gccagctatc
     5821 cgccggggca ttgttcaggc gcaggccaag gtgatccatg atgatttgct cgcaatagct
     5881 ctctttgggc ggctcatcga actgcggatc ggcattcagc gaaagctgga gtacgccttc
     5941 gttacgccca cggaacatcg ccagcgcgcg gtgagaaggc accgtggaca acggttcgtg
     6001 atgatcgaaa tagtcgcgga atttcgcccc ttcctcttct ttaccgctca ccaccgtaga
     6061 aaccaaatgc gcgttcttcc acagataatc acgcactttc gccagcagcg tggcatcttc
     6121 ggcaaaccgt tccatcagga tatagcgcgc gccatccagc gcggctttgg tatctgccac
     6181 gcctttatcg gcatcaacat attgtgcagc ggcgacttct ggcgtgtgtg acggatcgct
     6241 ccacagcagg tcagccaacg gctcaagccc tgcttcaatg gcgatttgcc cgcgggtgcg
     6301 gcgtttaggt ttgtagggca ggtagaggtc ttcgagttcg gttttgctta gggtggcgtt
     6361 gatggccttc gccagatcat cggtgagttt gccttgctcg gaaatggatt tgaggatcgc
     6421 ctgacgtctc tcttccagct cgcgcagata gctcagacgc gtttccagat tacgcagctg
     6481 cgtgtcatcc agaccgccgg tgatttcctt acgataacgt gcgataaacg gcacggtatt
     6541 cccttcgtca agcaggcgaa cggcagcgtc aacctgttcc gggcgcgcct gaatttcacc
     6601 cgcaataatg cggcagaacg aatcattcat catggtttga gttcatcttt tcggatcaaa
     6661 aatcagggga tagttatacg gactggctgg caaaaatgcc agccatcggc aggaggttaa
     6721 gactcttcct tacggtttca cgtactcgat agcattaaca taccagttcg cttccccggc
     6781 aggggtattc accaccgcca gatcgccgac ttcttttttc agcaatgcgc gggccatcgg
     6841 ggaatcgata gagatgtaat ctttacggcc aaaaatttca tcgtagccga caatacggaa
     6901 acggtgagtc acgccatcgt cgttttcaat ctccacccac gcgccaaaaa agactttgcc
     6961 ttcctgctga ggggagtaat cgacgatttt gagattttcc aggcatttag tgagatagcg
     7021 cacgcgacgg tcgatttcac gcagacgctt tttattatac tgatagtcag cattttcgct
     7081 gcggtcgccc agacttgcgg cccaggtcac cttttttgtg acctccgggc gttcttcacg
     7141 ccagagataa ttaagctctt gtttgagttt ttcataccct tcccgggtaa ccaggggcgt
     7201 tttcatctcg ttgattccct ttgtctgttt gataatgcgc acattgggta taacgtgatc
     7261 atatcaacag aatcaataat gtttcgccga ataaattgta tacttaagct gctgtttaat
     7321 atgctttgta acaatttagg ctgaaattca taccagattt agctggtgac gaacgtgagc
     7381 ttttttaaga atacacgctt acaaattgtt gcgaaccttt gggagtacaa acaatgcaag
     7441 agaactacaa gattctggtg gtcgatgacg acatgcgcct gcgtgcgctg ctggaacgtt
     7501 atctcaccga acaaggcttc caggttcgaa gcgtcgctaa tgcagaacag atggatcgcc
     7561 tgctgactcg tgaatctttc catcttatgg tactggattt aatgttacct ggtgaagatg
     7621 gcttgtcgat ttgccgacgt cttcgtagtc agagcaaccc gatgccgatc attatggtga
     7681 cggcgaaagg ggaagaagtg gaccgtatcg taggcctgga gattggcgct gacgactaca
     7741 ttccaaaacc gtttaacccg cgtgaactgc tggcccgtat tcgtgcggtg ctgcgtcgtc
     7801 aggcgaacga actgccaggc gcaccgtcac aggaagaggc ggtaattgct ttcggtaagt
     7861 tcaaacttaa cctcggtacg cgcgaaatgt tccgcgaaga tgagccgatg ccgctcacca
     7921 gcggtgagtt tgcggtactg aaggcactgg tcagccatcc gcgtgagccg ctctcccgcg
     7981 ataagctgat gaaccttgcc cgtggtcgtg aatattccgc aatggaacgc tccatcgacg
     8041 tgcagatttc gcgtctgcgc cgcatggtag aagaagatcc agcgcatccg cgttacattc
     8101 agaccgtttg gggcctgggc tacgtctttg taccggacgg ctctaaagca tgaggcgatt
     8161 gcgcttctcg ccacgaagtt catttgcccg tacgttattg ctcatcgtca ccttgctgtt
     8221 cgccagcctg gtgacgactt atctggtggt gctgaacttc gcgattttgc cgagcctcca
     8281 gcagtttaat aaagtcctcg cgtacgaagt gcgtatgttg atgaccgaca aactgcaact
     8341 ggaggacggc acgcagttgg ttgtgcctcc cgctttccgt cgggagatct accgtgagct
     8401 ggggatctct ctctactcca acgaggctgc cgaagaggca ggtctgcgtt gggcgcaaca
     8461 ctatgaattc ttaagccatc agatggcaca gcaactgggc ggcccgacgg aagtgcgcgt
     8521 tgaggtcaac aaaagttcgc ctgtcgtctg gctgaaaacc tggctgtcgc ccaatatctg
     8581 ggtacgcgtg ccgctgaccg aaattcatca gggcgatttc tctccgctgt tccgctatac
     8641 gctggcgatt atgctattgg cgataggcgg ggcgtggctg tttattcgta tccagaaccg
     8701 accgttggtc gatctcgaac acgcagcctt gcaggttggt aaagggatta ttccgccgcc
     8761 gctgcgtgaa tatggcgctt cggaggtgcg ttccgttacc cgtgccttta accatatggc
     8821 ggctggtgtt aagcaactgg cggatgaccg cacgctgctg atggcggggg taagtcacga
     8881 cttgcgcacg ccgctgacgc gtattcgcct ggcgactgag atgatgagcg agcaggatgg
     8941 ctatctggca gaatcgatca ataaagatat cgaagagtgc aacgccatca ttgagcagtt
     9001 tatcgactac ctgcgcaccg ggcaggagat gccgatggaa atggcggatc ttaatgcagt
     9061 gctcggtgag gtgattgctg ccgaaagtgg ctatgagcgg gaaattgaaa ccgcgcttta
     9121 ccccggcagc attgaagtga aaatgcaccc gctgtcgatc aaacgcgcgg tggcgaatat
     9181 ggtggtcaac gccgcccgtt atggcaatgg ctggatcaaa gtcagcagcg gaacggagcc
     9241 gaatcgcgcc tggttccagg tggaagatga cggtccggga attgcgccgg aacaacgtaa
     9301 gcacctgttc cagccgtttg tccgcggcga cagtgcgcgc accattagcg gcacgggatt
     9361 agggctggca attgtgcagc gtatcgtgga taaccataac gggatgctgg agcttggcac
     9421 cagcgagcgg ggcgggcttt ccattcgcgc ctggctgcca gtgccggtaa cgcgggcgca
     9481 gggcacgaca aaagaagggt aaataaacgg gaggcgaagg tgcctcccgt tttgctttct
     9541 ataagatact ggatagatat tctccagctt caaatcatta cagtttcgga ccagccgcta
     9601 ccagcgcggc acccgcaggg gtgtcggtgt atttatcgaa gttgtcgata aacagtttcg
     9661 ccagggtttc ggctttttcc tgccactgtt ccggagaagc gtaggtgtta cgcggatcga
     9721 gaatcttcgt gtctacgccc ggcagttcgg ttgggatcgc caggttaaac atcggcagag
     9781 tgaaggtttc tgcattatcc agcgaaccgt tgaggatggc gtcgataatg gcgcgggtat
     9841 ctttaatcga gatacgtttg ccagtgccgt tccagccagt gttaaccaga taagcctgcg
     9901 cgcccgccgc ctgcatacgt ttcaccagca cttctgcgta ctgagtcggg tgcagcgaca
     9961 ggaacgccgc gccgaagcaa gcggagaagg ttggcgtcgg ttcggtgatg ccacgctcag
    10021 taccggccag tttggcggtg aagccagaga ggaagtgata ctgggtttga tcggcagtca
    10081 ggcgagaaac cggcggcaac acgccgaaag catcagcagt caggaagata accttagtcg
    10141 cgtggcccgc tttggaaacc ggcttaacga tgttatcgat gtgatagatt ggataagaaa
    10201 cgcgggtgtt ctcggttttt gaaccatcat caaagtcgat agtgccatct tcacgcacgg
    10261 tgacgttttc cagcagcgca tcacgacgga tagcgttgta gatttcaggt tccgcttctt
    10321 tcgacagctt gatagttttt gcgtagcagc cgccttcgaa attaaacacg ccgtcatcgt
    10381 cccagccgtg ttcgtcatcg ccaatcaggc ggcgtttcgg gtcggtggaa agggtggttt
    10441 taccggtgcc ggaaaggccg aagaacaccg caacatcgcc tttctcgcca acgttggcgg
    10501 agcagtgcat agaagcgata cctttcagcg gcagcaggta gttcatcatc gagaacatcc
    10561 ctttcttcat ttcgccgccg taccaggtgc cgccaatcag ctgcatgcgc tcggtcaggt
    10621 taaacgccac gaagttttcg gagttcagac cctgttcttt ccactgcggg ttagtgcact
    10681 tcgcgccgtt cataacgata aagtctggtt tgaaacctgc cagctcttca tcgctcgggc
    10741 gaataaacat gtttttgaca aaatgcgcct gccaggccac ttcggtgatg aaacggacgg
    10801 aaagacgagt atccgggttc gcaccacaga aagcgtcgac aacgaacaga cgtttgccgg
    10861 aaagctgcct ggtcaccagg cctttcagat gctgccaggt ttccggagag agaggtttgt
    10921 tgtcgttctt acctttgcct ttgtctgccc accagaaagt atcgcgagtg gtatcgtcac
    10981 ggacgatata cttatctttt ggtgaacgac cggtgaagat cccggtatcg acggcaacgg
    11041 cacccagatt agttaacacc ccgcgctcat aacctgtcag gctcggatcg agctcttcct
    11101 gatacagcag gtcgtagctt gggttgtaaa cgatatcatg tacgtcactg ataccataag
    11161 cctcgagttc ttgcgggggc aaaccattgt taacgcgcat ttcactgctc cttagccaat
    11221 atgtattgcc tgaatagtaa agtctttttg ggggtgttaa ccgcgacaag gctcatagat
    11281 ttacgtatct ggagaaattc atatttgaca aaaaactccg tgattcctgt cacgaaacgg
    11341 ttgctattat cgcagaaata gcattttagg tgtggaaaat gttctcaaaa ggttaaatct
    11401 tggtttatat ggcgggaata ttgtgagtgg aatcgcattc ctgaatgaaa ttgatgcaaa
    11461 ttcaaccggt aaagtatcga ttcaccacgc taactttttt tgattatttt ctgaaaaaag
    11521 caaaatacta ctgcccggat gcgataatta acccaccttt tgattctgga taaccaacat
    11581 ggataacgtt gaactttcac cggcaacacg ctgggggatg attgctaccg gattacttca
    11641 ggggatgatt gctaccggat tacttcaggg gctggtttgc tacctgctta tcgcctggct
    11701 tgccggaaaa aatcacagtt ggattgttta tggcgtgcct gcgaccgtcg cgttttcatc
    11761 cgttttactc ttctccgtga tctcttttaa acagaagcgc ctttggggat ggctggcgct
    11821 ggtgtttatt gccacgacag ggatgagcgg ttggctgaag tggcaaactg atggcatgaa
    11881 cccctggaga gctgaaaagg cgatttggga ttttggctgc tatctgctgt tgatggcaat
    11941 gttgttgcta ccgtggatac aacaaagcct tcgtatccgc aatggcagta gccgctatag
    12001 ctacttttac cagtcagtat ggcataacgt acttatatta ctggtgattt ttctctccaa
    12061 tggcctgacg tggctggtgc ttttactgtg gggtgagttg tttaaacttg ttggcatcac
    12121 gtttttcaat acgctctttt ttgcaaccga ctggtttatg tatcttacat ttggtctggt
    12181 taccgcactg gcggtgatcc tcgcgcgtac acagtcacgt ttaatcgact ctattcaaaa
    12241 gttgttcacg ctaatcgcca cggggttgct gccgttagta tcattgctaa ccctgatgtt
    12301 tatcatcacc ctgccgttta cgggcctgag cgcgatttct cgccacatct ccgccgccgg
    12361 gttgctgtta acgctggcct ttttgcaatt gatcttaatg gctattgtcc gcgatccgca
    12421 aaaagcgtca cttccctgga cggggccgtt gcgttgcctg attaaaaccg ctttgctggt
    12481 tgctccgctg tatgtgttcg tcgccacctg ggggttatgg ctgcgggtcg ctcagtacgg
    12541 ctggactgtc gaccgcttgc agggcgcgct ggcggagctg gtgttactgg tgtggtcgct
    12601 ggggtatttt gtcagtatcg tctggcgtaa tgggcaaaat ccccttgttc ttcagggcaa
    12661 agtgaacctt gcggtttcgt tattggtgtt ggtgatactg gtgcttctta attcgccggt
    12721 gctggacagt atgcgtatta gcgtgaacag tcatatggcg cgttatcaga gcggcaaaaa
    12781 cacgccagac caggtaacta tctacatgct cgaacagagc ggtcgctatg gacgtgcggc
    12841 gcttgagtcg ctgaaaagcg atgccgggtt tatgaaagac ccgaaacgcg cgcgggatct
    12901 gctgatggcg ttagatggag agcaacatct tcaggaacag gtatcggaaa aagtattcgc
    12961 cgaaaatgtg ttaattgccc ctggttctgt taaaccggat gcgacattct ggtcggcctt
    13021 aatccaggat cgctataacg tgatgacctg tattgaaaaa gacgcctgcg tactggtcga
    13081 gcaagatctg aatagtgatg gtcaggcgga gcggatcctg tttgctttta atgatgacag
    13141 agtcattgtc tatggctttg actcagacag aaaagaatgg gacgcgcttg atatgagttt
    13201 acttccgaac gaaataacga aagaaaaatt actcacagct gcgaaggatg gaaaactggg
    13261 gacgaggccg aaagcgtggc gcgatctgac ggtagatggt gaaacgctgg aaataaactt
    13321 aagtaagtga caacgctttt atcggatacg acgctgtatc tgaccaaaat aatttgcgat
    13381 ttatttcata ggcgctggta agcgcagcac atcaggccat taagtcgtaa aatccggcag
    13441 agccctctct gccggacata ctcattaatg aacttacgga tctgccggag acgcgttgtt
    13501 gcggatttca gcaatatcca tcgcattgaa cagatagtgg ttaccgcagt aatcacaatg
    13561 catgtcaatt tcgccatctt ccgccaggat gctatcaact tcttcatcag gcagcgtttt
    13621 cagcgcatcg gcgcaacgtt cacgcgagca ggtgcatttg aactccacat cctgcggatc
    13681 gtaaaccgtc acctcttctt cgtgatacaa acgccacaac acttcgtttg ccggtaaggt
    13741 cagcagttct tcggttttga tggtttcggt tagcgtcgcc aggtggtcaa agtcgtcctg
    13801 ttgggtattt tgcgcaggca ttacctgcaa caacataccg cctgcagccg gtttgccgtc
    13861 tacgtcgccg gtgcgaataa acaggcgcgt cggcagctgt tcagaacgca taaagtaatc
    13921 ttctaggcag gccgccaggg tatcaccttc cagaccaact acgccctgat agcgttcgcc
    13981 ttcgctcggg gtaatggtga tcaccacgta accattgccg accagcgttt tcaggtcggc
    14041 attttctgga atttcgccct gcacgcgcgc cacaccgcgc atttgctggt tattgttacc
    14101 gttaataacc gccagattca tcggaccgtc gccctgcagc tgtacggtga tatcaccatc
    14161 aaacttcagc gtagcggtta acaggctggt cgcaaccagc agttctgcca gcacgttttt
    14221 aacgggctgc ggataatcgt ggttctcaag gatctgttgc agggtttccg aaacggttac
    14281 cagttcgccg cgaacggcaa aattttcaaa cagatagcga tgtaattggt catgttgcgg
    14341 cataatcatc tctcttgcag gtgacagtta ttcactgtcg ccgtgtttaa atcgtaacag
    14401 gtcgcggcgc tcttttttgt ccgggcgtcg gtccgggtgc ggcatggtta aggcattaag
    14461 tttacgtgcc atcgccattt tttcgcgttt ctctacactt tccgcagtct cttcatacag
    14521 caaggctgcc tcgctggcgg ggcgacgctg ttcagtaatc gcctttacaa tcaccgtgcg
    14581 ttcgtcattt ccctggcgca gagtgagcgt ggcattcagc tcgacgattt tgctcggctt
    14641 gctgcgctgc ccgttgtaat gcaccttacc gccttcaatc atttcacggg ccagcgcgcg
    14701 ggttttataa aaacgggcag cccatagcca tttatccagt cgaacctcaa cagcaggttt
    14761 ctctttcatg gcgtctcctt cacattagcg aggggatcag gcggcggtag tcattcagtg
    14821 acggatggcg ttgatactgt ttctcggcaa tcccggaatc aggattagtc acgccgaggc
    14881 agtaacgaat accaaattgc gcggcagcat cgagaatcgc ttcgctgtca tcaataaaca
    14941 gcgttctttc agctttcaga cccgtagctt cggccaccgc atgccataac cgctgatcct
    15001 ctttcggata accaaatgtg tgggtggaaa gtaataaatc aaggtgtgcg tccagacccg
    15061 tatgctcaag ttttaccgcc aggttgtgcg gatgagcatt ggtgagcaaa attcgctgct
    15121 taccgctggc tttcagtgcc tcaagaaacg gaatggtatc ttcacgcagt acggcgcgcg
    15181 gtcccatctc ggtggtcatc gcacagatat ccagacccag ttgctcactc cagtaatcaa
    15241 gacagtacca gtttagcgta tgctgtacgt cgtgatattg ctggcgcata tattccatcg
    15301 cttcctgtgg cgtaaccccg tttttcgcgc cccatgtttc aggcaccagc ttttgccaga
    15361 aatagttatc gaaggcgagg tcgagcaacg tgccgtccat atccagcaga acggtatcta
    15421 cgtcctgcca ggcaatgttg atatgcatga gggaaatctc cagagtgaag caatttgcgc
    15481 gacagggtag cataacctgc cgcgcaaacg tgttattcga taaggctttc tgaaggggtg
    15541 atcagttgcg ggttcaggca gctttcataa taggcctgaa tctccatcat gcgagtgcga
    15601 tgacgctggt aacgacgcca ggcctgtacg ccattgtaaa tggcgctacc cagcatcgtc
    15661 agtagcagca atgtggtgct gagatagcgc cacaggccgg aacgatccgg gatcggatga
    15721 aggccaatat gctgcgtacc attggcgtca gtgaagattt ttgtgacgat accttcggcg
    15781 ttaaacggcg tatgcatcag catttgtgcc agtttctgga aagcgttcca ctgttcttgc
    15841 ggcgggtagt cgtaaagtga tgccgaaggc cagggctgat caacaaaatc gctgccttcg
    15901 tcgctgacaa tcaggaatcc gccgggggcc ggactgttta gtgattgtgc cgctcgcgcc
    15961 gtttcatgcg tgataaacgg cgcggtggag gttgccacca ggttatccag cgattcagca
    16021 ctcaccgggc gtaataacac attcacgcca tcgagcttcc cggcgttggc gcgttttacc
    16081 agcgcgtccc agtctttact gttgccgaga ttgaccagcg catttttcag tcgcacacag
    16141 tcatctttgg cagaacataa atccgctgtc ttcagtacaa tgtcgccaaa atcatcaagc
    16201 aataccatgc cggatttttg aatcgctgaa cgtaatgagg cactgacgcg agattcatct
    16261 tccggtttag ggtgcagctg gcgattaact gcttcagtca atgccgtcgc tttgttgacc
    16321 agttcagatt ctggtaatgg taatgagcgg gcgtcattcc agatgatctg cgagcagtca
    16381 aacggtaaaa aaggtgaatt ggttttcgcg ctccaggttc cggaagtgcg aatattacac
    16441 attcccgtac cgctaatacg caatgtatcg cctacccgca cgccagcgtc agccagttgt
    16501 tttacgctgg tggcttcaat ggtctgcgcg cctttcatcc atgagagagt gaatttcagc
    16561 ggcatatcca gcgggatcca gaataacagc ataaacagca ctagcagcga gcccgcagcg
    16621 ataatcgtac tgcgcagcca gtgctgtaac ggaaagtttt ttacttcatc atgcagcgaa
    16681 agatatcgcc cctgacgcac tacgtggcgg tcgagataga tatcaatatc ggtttgttga
    16741 ccgagatcct gagcaatgta tggctgccag tgtgcgggat agaccaggtc gataataccg
    16801 agtgaaatat tgttgatctg ttcctgatcg ttttcgccaa acaatcccca acgacggggt
    16861 gtaccgcgca gacaatgaat ttctcgcagg gaggattttg ccgggggcgc gaatagcccc
    16921 cacagacctg cgcccagcag cagtaatgcg ccgcctgcca gccacggaac aaatacatcc
    16981 ggggtaatca ggcagaaaaa gaacatcagg aaggaggcga cgatcagcaa cgcttcacgc
    17041 agcccgcgcg gacgactcag cgcatattct tcatgcgttt ctttgcgaat attgagtagc
    17101 tcgatttgct cactctcttc cccgcgaatc gacgcctgcg tggacggaac aggttgcaag
    17161 gcatagccgc gcgtttcctg catgtactcc tgcagcgtat gaccgttgag tgaaataacc
    17221 aacggcagcg aatcggtatg aatcagttca acggtattct catcgttgat gtactgttcc
    17281 cagaaggggg gcaggtggac ttctaccgaa tcgaggtagt aacgccattt attcggatcg
    17341 tcggtagaga tgccgtaacg tgtgatagcg tgtgtcagca tcatgacgtt gttgctttcg
    17401 gcattcagcg ccagagagat cggtgccgcg ctagcttccg ttgggccagg cacctgtaat
    17461 acctgcgtca ggctctcaag ataattttca acggcgctac gttcttcagg tgtgagttta
    17521 cgcgtttgcg catccgcgaa ggcgttggtc cagggcagct gacgccgtct ggatcgcact
    17581 tttatcagcc atcccgcaag tagtgagcag gccagcaaag cagctaaaaa aatcacaatg
    17641 gtgctcatgc tttccccatc ttacttagtc tgtcaggcgt ggtcagtcgc accggacaca
    17701 atacatgatt ctgcggttgg ctatcggcaa gcgtggagct taacttgagc atcattaagc
    17761 acatcacagt aaccgctgat catagcaaac cctctgaagg cggaatatca gcaaattcct
    17821 ggagttattt caacctattg acttttaatt tgatttgagg acgattattt attaagttac
    17881 ataaatgtaa ataacatgca attcacattg ccgaaagcgt gtccgatatc gcacaataac
    17941 cgctgatttc acagcataga tcccacgatg aacaaatcat tacaaaaacc caccattctg
    18001 aatgttgaaa ctgtagcccg ttcccgactg tttaccgtcg agagcgtgga tctggagttc
    18061 agcaatggcg tgcggcgtgt ttatgaacga atgcgtccaa ccaaccggga agcagtgatg
    18121 attgtgccga ttgtggacga tcacctgatc ctgatccgcg aatacgcagt gggaactgaa
    18181 tcctacgaat taggtttttc gaaaggatta attgatccgg gtgaaagcgt ctacgaagcc
    18241 gctaaccgcg agctaaaaga agaggttgga tttggtgcga acgatctgac ttttttgaag
    18301 aagctcagca tggcaccgtc ttacttttcc agcaaaatga atatcgtggt cgcgcaagat
    18361 ctctacccgg aatcgctgga aggcgatgag ccagagccgc taccacaggt gcgctggccg
    18421 ctggcgcata tgatggattt gctggaagac cctgacttca acgaagcgcg taatgtcagc
    18481 gcgctgttcc tcgtgcgcga atggttgaaa gggcaggggc gagtgtaaat gcgtgaagtt
    18541 tgcctgatgc acaacgctta tcaggcttat ggggttcctg caatatattg atttttcacg
    18601 attttgttag ccggataacg cgttcacccg catccggcat aaacaaatgc actttgtcag
    18661 ccaactgaaa aggcgccgaa gcgccttttt aatcagaaca attcctgtgc ctcaccatta
    18721 tcgataatgg tcgttcccac ctcgtgcacc gcctgttgtg tcggctgcgt accttcgatg
    18781 aaatactctt cgcggctgtt gccaccatta gctaactgcc cggtgctgcg atcgatattc
    18841 accgtcacaa tacccggagg cggcgtcagc ggctgctccg gcacaccttc aagaacggct
    18901 ttcatataag cgtcccatgc aggctgggca ctcttggcac cgccttcgta acctgagatc
    18961 tgatctttaa tcgctccgga agccgttgta tgaccgagat tacgacggtg atcatcaaag
    19021 ccaatccaga ccgaggtcac aacgcccgga ccgtaacccg agaaccacgc atctttcgaa
    19081 ctgttagtgg taccggtttt cccgccgata tcgcgacgct gcaaatcacg acctgcacgc
    19141 cagccggtac cctgccagcc tggttcacca aagatattgg tgttcaaagc actcttaatc
    19201 aggaatgcca gcggagtgtt gatgacgtgc ggtgcgtact cctgcgcgcc agtcttcgcc
    19261 actaacgcct gatttgcctg ctccagctgc ggcattggta cagaaacatt ctgctgctcg
    19321 cgggagatag cgacatcttc aacatcgtta ttttccagca cgttcgattt ctgcgtatca
    19381 ccgtaaatca ctggaatatc gcattccggg caggctactt tcggtttcgc ttcgaaaatc
    19441 acgccgccct gatcgttttc aattttgctg ataaaccacg ggtccactag gaagccgccg
    19501 ttcgccatga ccgcgtagcc gcgcgccacc tgcattgggg tgaaggacgc tgaacccagc
    19561 gccagcgatt cggtgtggac aatgttttgc gccgggaagc cgaagcgttg cagatattct
    19621 gcagcgtagt cgacgcccat cgcccgcatt gcacgtacca tcaccacgtt tttcgactga
    19681 cccagcccct gacgtaagcg aattggacca gcatactgcg gtggtgagtt cttcggctgc
    19741 cagtcagaac cggcacctgc atcccagcga gaaattggca catcgttcaa catacttgcc
    19801 agcgtcagac ctttatccat cgccgcggtg tagaggaacg gtttgatgtt ggaacccacc
    19861 tgacgcagtg cctgggtggc gcggttaaac ttgctctgat tgaaatcaaa gccaccgacc
    19921 agcgccataa cggcaccatt ttgcggattg atcgacacca gcgccgagtt cacttccggc
    19981 acttgtgcca gccaccatgc atcgccaacc tgacgaaccc agatttgctg acccgtttgc
    20041 agaacatcgg tcactttacg cggcgtcggt ccctgctgag tatccgaacg gtaaggacgc
    20101 gcccagcgaa cgccttccat actcaatgcg acggtcgacc cgtccgccag catcgccgtc
    20161 gctccctgag gattggcgct ggtgactgcg gcaggcagca gcggaccata ggtcggcagc
    20221 gccttcagcg tatcggtaat cttgttgtta tcccacgccg actcgcccac tttccacagc
    20281 acatttgccg ggccgcgata gccgtggcgc atgtcgtagt ccagcacgtt attacgtacc
    20341 gcctgctgcg cggcctgctg cactttgcgg gtgatggtgg tgtaaatgcg ataaccgtct
    20401 tcataggcac tttcgccata acggttatac atctcctggc gcaccatttc gctcaggtac
    20461 ggcgcagaga aagcaatctc cggcgcgtga taattagcgt taatcgcctc agtgcgtgtc
    20521 tgatcgaact gttgctgggt gatataccct tcatccagca tccgcgacag tacgacgtta
    20581 cgccgcgcga cggcacgatc cattgagtag agcggattaa aggtggaagg cgctttcggc
    20641 aacccggcta tcaccgccat ttcgttcagc gtcagttggt cgaccgtttt accgaaatag
    20701 acttgtgccg cagcaccgac accataggcg cggtaaccaa ggtaaatctt gttcagataa
    20761 agctcgagga tttcgtcttt cgtcagcaac tgttcaatgc gaatcgcgag gaagacttcc
    20821 ttaatcttac gcatcatcgt gcgttctgga ctgaggaaga aatttctcgc cagctgctgg
    20881 gtaatggtac tcgccccttg tgacgcgtgg ccggagaaca gcgccacact tgccgcacgg
    20941 aagatcccca ccgggtcaac gccgtgatgc tcgtagaagc ggctgtcttc cgtcgcgata
    21001 aaggctttca ccatctccgg tgggatttga tccaacgtaa ccggaatacg acgtttctca
    21061 ccgtattgag caatcagctc gccatcggcg ctgtaaatct gcatcggaat ttgcaggcga
    21121 acatctttta atgtcaccac atccggcagt tgtggctcga tgtagcggta taggccataa
    21181 atcgagcctg ctcccagcag aatgcaacag actgcaagga tcaaaaaata ctttacgaac
    21241 ttcactggaa atttcccatt tagtttcatt tgggcagttt ataaacaaac gcgcggtagt
    21301 ataaaggcaa gccagacgca ttgatatacc cgtcagagtg acgggtgata aggagatcat
    21361 cacaatgaca tttaagatct ggcaaattgg tttgcattta caacagcaag aagcggcagc
    21421 ggttgcgatc gtgcggggcg caaaagaatg ctttttgcaa cgctggtggc ggttgccgct
    21481 ggcgcacgac attatcaaag atgggcggat tgttgatgcg cagcagctgg ctaaaacgtt
    21541 gttaccgtgg agtcgcgaac tgccgcagcg tcatcacatt atgttggcgt ttcccgccag
    21601 tcgcacatta cagcggtcat ttccgcgccc gtcgatgtcc cttggtgagc gggagcaaac
    21661 ggcctggctg tcagggacga tggcccgcga gctggatatg gatccggact ccctgcgctt
    21721 cgattacagc gaagactcac tcagccccgc ttataacgtg actgccgcgc aaagcaaaga
    21781 gctggcaacg ctgcttacgc tggcagaaag gttgcgtgtt catgttagtg cgatcacccc
    21841 ggatgccagt gcattacagc gattcctgcc ttttttacct tctcatcagc aatgtctggc
    21901 ctggcgtgat aacgaacagt ggctgtgggc gacgcgctat agctgggggc gcaaactggc
    21961 ggtggggatg actagcgcga aggagctggc ggcagcgtta tccgttgatc ccgacagcgt
    22021 cgcgatatgt ggcgaaggcg gatttgaccc ctgggaggcc gtttctgttc gtcagccgcc
    22081 gctaccgccg cctggtggag actttgccat cgcgctgggg ctggcgcttg ggaaggcgta
    22141 ctgatgaatc cgccaattaa ttttttgccc tggcgacagc aacgccggac cgcttttctg
    22201 cgtttctggt tgctgatgtt cgttgcgcct ctgctgctgg ccgtcgggat aacgctaata
    22261 ctgcgtctga caggcagcgc cgaagcgcgc atagacgcag ttttgcttca ggcggaacaa
    22321 caactcgccc gcagcttaca gataacgaag ccacgtttgc tggagcaaca gcagttacgc
    22381 gaacagcgtt cgcaacggca gcgccagcga caatttaccc gcgactggca atctgcgctg
    22441 gaagcactgg cggcgctttt acccgagcac gcctggctga caacgataag ctggcagcag
    22501 ggaacgctgg agatcaaggg gctgacaaca agcattaccg cgttaaacgc actagaaacg
    22561 tcactccgcc aggatgcgtc ttttcatctc aatcagcggg gagccacgca gcaggatgcg
    22621 cagggacgct ggcaatttga gtatcagtta acaaggaagg ttagcgatga acatgttctt
    22681 tgactggtgg ttcgccacat caccccgcct ccgccagttt tgctgggcat tctggttgct
    22741 gatgttagtt acgctcattt ttctatcatc gacacaccat gaagagcgcg acgcattaat
    22801 tcgactacgg gcaagtcatc accagcagtg ggccgcactg tatcgcctgg tagacaccac
    22861 tcccttcagc gaggaaaaaa cgctgccctt ttcgccactg gattttcagt tatccggcgc
    22921 gcaactggtt tcctggcatc catccgcgca gggaggtgag ttggcgttga aaacgctgtg
    22981 ggaagcagtg ccgtcggcat ttacacggct ggcagagcgc aacgtcagcg tgagccgttt
    23041 ttcgttaagc gtggaaggtg atgatctttt gttcacgcta caactggaga tgccgcatga
    23101 gggttaaacg ctggttgttg gcaggtattg cattgtgcct tttaaccggt atgcgtgacc
    23161 cttttaaatc gccggaagat ctatatgccg gattagcgaa cttagccagt ggcgctatca
    23221 ggggatggta gggcgaggcg agcgcatcat cggtgtaata aaagacgggc aaaagaaatg
    23281 gcgacgggtg cagcaaaacg atgtgctgga aaacggctgg acaattttac agctgacgcc
    23341 agacgtacta acgctgggta ccgggacaaa ctgcgaaccg ccacaatggt tgtggcaacg
    23401 gcaaggagat acaaatgaag caatggatag ccgcactact gttgatgctg atacccggcg
    23461 tacaggcggc aaagccgcaa aaagtgacgc tgatggtgga tgacgttccg gtagctcagg
    23521 tgttgcaggc gctggctgaa caggagaagt tgaacctggt cgtgtcgcca gacgtcagcg
    23581 gtacggtgtc gttacatcta acagatgttc cctggaagca ggcactacaa actgtagtga
    23641 aaagcgccgg actgataacg cggcaggaag gcaacattct ctcagtgcat tccattgcct
    23701 ggcagaatga caatatcgcc cgccaggagg cggagcaggc gcgggcgcag gcaaatctgc
    23761 cgctggaaaa tcgcagtata accctgcaat acgccgacgc gggagaactg gcgaaagcgg
    23821 gggagaagct actgagtgcc aaagggagta tgaccgtcga taaacgcacc aatcgccttt
    23881 tgctacgaga taacaaaacg gcgttaagcg cgcttgaaca gtgggtagcg caaatggatc
    23941 tgccggtcgg gcaggttgag ctgtcggcgc atattgtcac cattaatgaa aaaagtttgc
    24001 gtgagttagg cgtgaaatgg acgctggccg atgcgcaaca cgctggtggc gttgggcaag
    24061 tcaccacgct tggtagcgac ctctccgtag cgacggcgac aacgcatgtc ggttttaaca
    24121 ttgggcgcat caacggacgc ttgctggatc ttgagctttc cgcgctcgaa caaaaacagc
    24181 agctggatat tatcgccagt ccgcgtctgc tggcctcaca tcttcagcct gccagcatta
    24241 aacaggagag cgaaattcca tatcaggttt ccagcgggga aagtggcgcg acgtcggtgg
    24301 aatttaaaga ggccgtcctg gggatggagg tcacgcccac ggtgttacaa aaaggtcgca
    24361 tccggctgaa attacacatc agccagaacg ttccggggca ggtgctacag caggccgatg
    24421 gcgaagtgct ggcgattgat aagcaggaga tcgaaacgca ggtcgaggta aaaagcggag
    24481 aaacgttggc gctgggcggc atttttaccc gtaaaaataa atcgggtcag gatagcgtac
    24541 cgttgcttgg cgacattccc tggttcgggc aattatttcg tcatgacgga aaagaagatg
    24601 aacgacgcga gttagtggtg tttatcacgc cacgactggt ttccagtgag taaacagccg
    24661 taaaagcggt aatgttttta tgctgaacgt gtttcatcta tttgacgcgc gcaggtattt
    24721 agcatacaag gagtaccgat ttgagagttg gtgctcttcg ctgcctgcgt tccatgatga
    24781 tgatttatca ttcaggcggc attttgctgt cttttttacg ctaatcttac ccggcggtga
    24841 tttatcgcca gagcggtggt agcaaggcag cgcgcttgca gcgaccagat atgcagaggg
    24901 atgggtgatt tattcagttg ccaaacccgc tggagtattg agataatttt cagtctgact
    24961 ctcgcaatat cttatgaggt ttcagttcat gtcctgcggc gctctctgag cgaagcgggt
    25021 ttatcattaa cgaatagtct tagtagtacc gaaaaaatgg cagagaaacg caatatcttt
    25081 ctggttgggc ctatgggtgc cggaaaaagc actattgggc gccagttagc tcaacaactc
    25141 aatatggaat tttacgattc cgatcaagag attgagaaac gaaccggagc tgatgtgggc
    25201 tgggttttcg atttagaagg cgaagaaggc ttccgcgatc gcgaagaaaa ggtcatcaat
    25261 gagttgaccg agaaacaggg tattgtgctg gctactggcg gcggctctgt gaaatcccgt
    25321 gaaacgcgta accgtctttc cgctcgtggc gttgtcgttt atcttgaaac gaccatcgaa
    25381 aagcaacttg cacgcacgca gcgtgataaa aaacgcccgt tgctgcacgt tgaaacaccg
    25441 ccgcgtgaag ttctggaagc gttggccaat gaacgcaatc cgctgtatga agagattgcc
    25501 gacgtgacca ttcgtactga tgatcaaagc gctaaagtgg ttgcaaacca gattattcac
    25561 atgctggaaa gcaactaatt ctggctttat atacactcgt ctgcgggtac agtaattaag
    25621 gtggatgtcg cgttatggag aggattgtcg ttactctcgg ggaacgtagt tacccaatta
    25681 ccatcgcatc tggtttgttt aatgaaccag cttcattctt accgctgaaa tcgggcgagc
    25741 aggtcatgtt ggtcaccagc gaaaccctgg ctcctctgta tctcgataag gtccgcggcg
    25801 tacttgaaca ggcgggtgtt aacgtcgata gcgttatcct ccctgacggc gagcagtata
    25861 aaagcctagc tgtactcgat accgtcttta cggcgttgtt acaaaagccg catggtcgcg
    25921 atactacgct ggtggcgctt ggcggcggcg tagtgggcga tctgaccggc ttcgcggcgg
    25981 cgagttatca gcgcggtgtt cgtttcattc aagtcccgac gacgttactg tcgcaggtcg
    26041 attcctccgt tggcggcaaa actgcggtca accatcccct cggtaaaaac atgattggcg
    26101 cgttctacca gcctgcttca gtggtggtgg atctcgactg tctgaaaacg cttcccccgc
    26161 gtgagttagc gtcggggctg gcagaagtca tcaaatacgg cattattctt gacggtgcgt
    26221 tttttaactg gctggaagag aatctggatg cgttgttgcg tctggacggt ccggcaatgg
    26281 cgtactgtat tcgccgttgt tgtgaactga aggcagaagt tgtcgccgcc gacgagcgcg
    26341 aaaccgggtt acgtgcttta ctgaatctgg gacacacctt tggtcatgcc attgaagctg
    26401 aaatggggta tggcaattgg ttacatggtg aagcggtcgc tgcgggtatg gtgatggcgg
    26461 cgcggacgtc ggaacgtctc gggcagttta gttctgccga aacgcagcgt attataaccc
    26521 tgctcacgcg ggctgggtta ccggtcaatg ggccgcgcga aatgtccgcg caggcgtatt
    26581 taccgcatat gctgcgtgac aagaaagtcc ttgcgggaga gatgcgctta attcttccgt
    26641 tggcaattgg taagagtgaa gttcgcagcg gcgtttcgca cgagcttgtt cttaacgcca
    26701 ttgccgattg tcaatcagcg taacaacaag aaaggtcagg ccgcttatca agcggtctat
    26761 tagcttcagg ttaattgcaa cgtggtaagc attaaccttt tagtggggtg ttaaatggat
    26821 gaattcaaac cagaagacga gctgaaaccc gatcccagcg atcgtcgtac tggtcgttct
    26881 cgtcaatctt ctgaacgttc tgagcgtact gaacgtggcg aaccgcagat caattttgat
    26941 gatattgaac ttgatgacac tgacgatcgc cgtccgactc gtgcgcaaaa agagcgcaat
    27001 gaggaaccgg aaatcgaaga agaaattgac gaatccgaag atgaaaccgt ggatgaagag
    27061 cgcgtagagc gtcgtccgcg taagcgcaaa aaagcagcca gtaaacccgc ttctcgtcag
    27121 tatatgatga tgggcgtcgg cattctggtt ctactgctgt tgatcatcgg tatcggttct
    27181 gcgctaaaag ccccctcgac cacttccagc gatcaaaccg cgtctggcga gaagagtatt
    27241 gatcttgctg gcaatgcgac cgatcaggcg aatggcgtgc agccagcgcc gggaaccacg
    27301 tctgcggaaa atactcagca ggatgtttct ctgccaccga tctcttctac gccgactcaa
    27361 gggcaaaccc cggtggcaac ggatggtcaa caacgtgttg aagtgcaggg tgacctgaac
    27421 aatgcgctga cccagccaca aaatcagcaa cagctgaaca atgtggcggt caattccacg
    27481 ttgccgactg aacccgcaac ggttgcgcct gttcgcaatg gcaatgcatc gcgtgacacg
    27541 gcgaaaacgc aaaccgctga acgtccgtcc actacgcgcc cagttcgtca gcaggcggtg
    27601 attgaaccga aaaaaccgca agcaaccgtg aaaacggagc cgaagccggt agcacagacg
    27661 ccgaagcgta ctgaacctga accagctgcc cctgtggcga gcacgaaggc accggctgcg
    27721 acttctacgc cagcaccaaa agagacggcg actacggctc cagtacagac ggcatccccg
    27781 gcgcaaacca cggcaacacc agccgctgga gggaagaccg caggtaatgt tggttcgttg
    27841 aaatcggcac cgtccagcca ttacactctg cagctgagca gttcctctaa ctacgacaac
    27901 ctgaacggtt gggcgaagaa agagaatctg aaaaactacg ttgtctatga aacgacgcgt
    27961 aatggtcagc cgtggtatgt cctggtttct ggcgtgtacg cttcgaaaga agaggcgaaa
    28021 aaagcggtat ctacattgcc agcagatgtt caggccaaaa acccgtgggc gaaaccgctg
    28081 cgtcaggtac aggccgatct gaagtaatca aggttatctc ccgcaatggt ttatcgttgc
    28141 gggagctgcc tgaagcgctg gatgctgtcg gagctttctc cacagccgga gaaggtgtaa
    28201 ttagttaatc ggcatgaaga aaaatcgcgc ttttttgaag tgggcagggg gcaagtatcc
    28261 cctgcttgat gatattaaac ggcatttgcc caagggcgaa tgtctggttg agccttttgt
    28321 aggtgccggg tcggtgtttc tcaacaccga cttttctcgt tatatccttg ccgatatcaa
    28381 tagcgacctg atcagtctct ataacattgt gaagatgcgt actgatgagt acgtacaggc
    28441 cgcacgcgag ctgtttgttc ccgaaacaaa ttgcgccgag gtttactatc agttccgcga
    28501 agagttcaac aaaagccagg atccgttccg tcgggcggta ctgtttttat atttgaaccg
    28561 ctacggttac aacggcctgt gtcgttacaa tctgcgcggt gagtttaacg tgccgtttgg
    28621 ccgctacaaa aaaccctatt tcccggaagc agagttgtat cacttcgctg aaaaagcgca
    28681 gaatgccttt ttctattgtg agtcttacgc cgatagcatg gagcgcgcag atgatgcatc
    28741 cgtcgtctat tgcgatccgc cttatgcacc gctgtctgcg accgccaact ttacggcgta
    28801 tcacacaaac agttttacgc ttgaacaaca agcgcatctg gcggagatcg ccgaaggtct
    28861 ggttgagcgc catattccag tgctgatctc caatcacgat acgatgttaa cgcgtgagtg
    28921 gtatcagcgc gcaaaattgc atgtcgtcaa agttcgacgc agtataagca gcaacggcgg
    28981 cacacgtaaa aaggtggacg aactgctggc tttgtacaaa ccaggagtcg tttcacccgc
    29041 gaaaaaataa ttctcaagga gaagcggatg aaacagtatt tgattgcccc ctcaattctg
    29101 tcggctgatt ttgcccgcct gggtgaagat accgcaaaag ccctggcagc tggcgctgat
    29161 gtcgtgcatt ttgacgtcat ggataaccac tatgttccca atctgacgat tgggccaatg
    29221 gtgctgaaat ccttgcgtaa ctacggcatt accgccccta tcgacgtaca tctgatggtg
    29281 aaacccgtcg atcgcattgt gcctgatttc gctgccgctg gtgccagcat cattaccttt
    29341 catccagaag cctccgagca tgttgaccgc acgctgcaac tgattaaaga aaatggctgt
    29401 aaagcgggtc tggtatttaa cccggcgaca cctctgagct atctggatta cgtgatggat
    29461 aagctggatg tgatcctgct gatgtccgtc aaccctggtt tcggcggtca gtctttcatt
    29521 cctcaaacac tggataaact gcgcgaagta cgtcgccgta tcgacgagtc tggctttgac
    29581 attcgtctgg aagtggacgg tggcgtgaag gtgaacaaca ttggcgaaat cgctgcggcg
    29641 ggcgcggata tgttcgtcgc cggttcggca atcttcgacc agccagacta taaaaaagtc
    29701 attgatgaaa tgcgcagtga actggcaaag gtaagtcatg aataagtttg aagatattcg
    29761 cggcgtcgct tttgatcttg atggtacgct ggtcgacagt gctcctggtc ttgctgctgc
    29821 ggtagatatg gcgctgtatg cgctggagtt gcccgtcgca ggtgaagaac gcgttattac
    29881 ctggattggt aacggcgcag atgttctgat ggagcgcgca ttgacctggg cgcgtcagga
    29941 acgtgcgact ctgcgtaaaa caatggggaa accgcccgtt gatgacgaca ttccggcaga
    30001 agaacaggta cgtattctgc gtaaactgtt cgatcgctac tatggcgagg ttgccgaaga
    30061 ggggacgttt ttgttcccgc acgttgccga tacgttgggc gcgttgcagg ctaaaggcct
    30121 gccgctaggc ttggtcacca acaaaccgac gccgttcgtc gcgccgctgc tcgaagcctt
    30181 agatattgcc aaatacttta gcgtggttat cggcggcgat gatgtgcaaa acaaaaaacc
    30241 gcatccggac ccgctgttac tggtggctga gcggatggga attgccccac aacagatgct
    30301 gtttgtcggc gactcacgga atgatattca ggcggcaaaa gcggcaggtt gcccatcagt
    30361 tggcttaacc tacggataca actacggcga ggctatcgat ctcagccagc ctgatgtaat
    30421 ttatcagtct ataaatgacc ttctgcccgc attagggctt ccgcatagcg aaaatcagga
    30481 atcgaaaaat gactaagccc atcgttttta gtggcgcaca gccctcaggt gaattgacca
    30541 ttggtaacta catgggtgcg ctgcgtcagt gggtaaacat gcaggatgac taccattgca
    30601 tttactgtat cgttgaccaa cacgcgatca ccgtgcgcca ggatgcacag aagctgcgta
    30661 aagcgacgtt ggatacgctg gccttgtatc tggcttgtgg tatcgatcct gagaaaagca
    30721 ccatttttgt tcagtcccac gtaccagaac atgcgcagtt aggctgggca ctgaactgct
    30781 atacctactt cggcgaactg agtcgcatga cgcagtttaa agataaatct gcgcgttatg
    30841 ccgagaacat caacgctggt ctgtttggct atccggtgct gatggctgcg gacatcctgc
    30901 tgtatcaaac taatctggta ccggtgggtg aagaccagaa acagcacctg gaactgagtc
    30961 gcgatatcgc ccagcgtttc aacgcgctgt atggcgagat ctttaaggtg ccggagccgt
    31021 ttattccgaa atctggcgcg cgcgtaatgt cgctgctgga gccgaccaag aagatgtcca
    31081 agtctgacga taaccgcaat aacgttatcg gcctgctgga agatccgaaa tcggtagtga
    31141 agaaaatcaa acgcgcggtc actgactccg acgagccgcc ggtagttcgc tacgatgtgc
    31201 agaacaaagc gggcgtttcc aacctgctgg atatcctttc tgcggtaacg ggccagagca
    31261 tcccagaact ggaaaaacag ttcgaaggca agatgtatgg tcatctgaaa ggtgaagtgg
    31321 ctgatgccgt ttccggtatg ctgactgaat tgcaggaacg ctatcaccgt ttccgcaacg
    31381 atgaagcctt cctgcaacag gtgatgaaag acggcgcaga aaaagccagt gtacacgctt
    31441 cccgtacgct gaaagcggtg tacgaagcga ttggttttgt ggcgaagccg taat
//
LOCUS       DAZSNM010000017        31446 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZSNM010000017 DAZSNM010000000
VERSION     DAZSNM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31446
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.017"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="ACHW42_000740"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="ACHW42_000740"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR8942683.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="ACHW42_000741"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="ACHW42_000741"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR8942684.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHW42_000742"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHW42_000742"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR8942685.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHW42_000743"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHW42_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR8942686.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHW42_000744"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHW42_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR8942687.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHW42_000745"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHW42_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR8942688.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHW42_000746"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHW42_000746"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR8942689.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="ACHW42_000747"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="ACHW42_000747"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR8942690.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="ACHW42_000748"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="ACHW42_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR8942691.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHW42_000749"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHW42_000749"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR8942692.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHW42_000750"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHW42_000750"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR8942693.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="ACHW42_000751"
     CDS             8212..9075
                     /locus_tag="ACHW42_000751"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR8942694.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="ACHW42_000752"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="ACHW42_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR8942695.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="ACHW42_000753"
     CDS             10411..11028
                     /locus_tag="ACHW42_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR8942696.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHW42_000754"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHW42_000754"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR8942697.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="ACHW42_000755"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="ACHW42_000755"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR8942698.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHW42_000756"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHW42_000756"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR8942699.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="ACHW42_000757"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="ACHW42_000757"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR8942700.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="ACHW42_000758"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="ACHW42_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR8942701.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="ACHW42_000759"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="ACHW42_000759"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR8942702.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHW42_000760"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHW42_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR8942703.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="ACHW42_000761"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="ACHW42_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR8942704.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="ACHW42_000762"
     CDS             21859..23568
                     /locus_tag="ACHW42_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR8942705.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHW42_000763"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHW42_000763"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR8942706.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="ACHW42_000764"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="ACHW42_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="ACHW42_000765"
     tRNA            27625..27719
                     /locus_tag="ACHW42_000765"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="ACHW42_000766"
     CDS             27991..29202
                     /locus_tag="ACHW42_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8942707.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="ACHW42_000767"
     CDS             29614..30657
                     /locus_tag="ACHW42_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR8942708.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31172..>31446
                     /locus_tag="ACHW42_000768"
     CDS             31172..>31446
                     /locus_tag="ACHW42_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942709.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIVT"
BASE COUNT         7881 a         8288 c         7801 g         7476 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
    31141 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
    31201 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
    31261 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
    31321 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
    31381 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
    31441 tgttac
//
LOCUS       DAZSNM010000018        30905 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZSNM010000018 DAZSNM010000000
VERSION     DAZSNM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30905
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.018"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            109..1767
                     /locus_tag="ACHW42_000769"
     CDS             109..1767
                     /locus_tag="ACHW42_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR8942710.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1764..2720)
                     /locus_tag="ACHW42_000770"
     CDS             complement(1764..2720)
                     /locus_tag="ACHW42_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR8942711.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2871..3986
                     /gene="macA"
                     /locus_tag="ACHW42_000771"
     CDS             2871..3986
                     /gene="macA"
                     /locus_tag="ACHW42_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR8942712.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3983..5929
                     /gene="macB"
                     /locus_tag="ACHW42_000772"
     CDS             3983..5929
                     /gene="macB"
                     /locus_tag="ACHW42_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR8942713.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6002..6226)
                     /gene="cspD"
                     /locus_tag="ACHW42_000773"
     CDS             complement(6002..6226)
                     /gene="cspD"
                     /locus_tag="ACHW42_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR8942714.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6376..6450
                     /gene="yljB"
                     /locus_tag="ACHW42_000774"
     CDS             6376..6450
                     /gene="yljB"
                     /locus_tag="ACHW42_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR8942715.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6549..6869
                     /gene="clpS"
                     /locus_tag="ACHW42_000775"
     CDS             6549..6869
                     /gene="clpS"
                     /locus_tag="ACHW42_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR8942716.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6900..9176
                     /gene="clpA"
                     /locus_tag="ACHW42_000776"
     CDS             6900..9176
                     /gene="clpA"
                     /locus_tag="ACHW42_000776"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR8942717.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9579..9666)
                     /locus_tag="ACHW42_000777"
     tRNA            complement(9579..9666)
                     /locus_tag="ACHW42_000777"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9630..9632),aa:Ser,seq:gga)
     gene            complement(9920..10138)
                     /gene="infA"
                     /locus_tag="ACHW42_000778"
     CDS             complement(9920..10138)
                     /gene="infA"
                     /locus_tag="ACHW42_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR8942718.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10423..11127)
                     /gene="aat"
                     /locus_tag="ACHW42_000779"
     CDS             complement(10423..11127)
                     /gene="aat"
                     /locus_tag="ACHW42_000779"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR8942719.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11169..12890)
                     /gene="cydC"
                     /locus_tag="ACHW42_000780"
     CDS             complement(11169..12890)
                     /gene="cydC"
                     /locus_tag="ACHW42_000780"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR8942720.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12891..14657)
                     /gene="cydD"
                     /locus_tag="ACHW42_000781"
     CDS             complement(12891..14657)
                     /gene="cydD"
                     /locus_tag="ACHW42_000781"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR8942721.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14780..15745)
                     /gene="trxB"
                     /locus_tag="ACHW42_000782"
     CDS             complement(14780..15745)
                     /gene="trxB"
                     /locus_tag="ACHW42_000782"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR8942722.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16289..16783
                     /gene="lrp"
                     /locus_tag="ACHW42_000783"
     CDS             16289..16783
                     /gene="lrp"
                     /locus_tag="ACHW42_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR8942723.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16918..20946
                     /gene="ftsK"
                     /locus_tag="ACHW42_000784"
     CDS             16918..20946
                     /gene="ftsK"
                     /locus_tag="ACHW42_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR8942724.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21101..21712
                     /gene="lolA"
                     /locus_tag="ACHW42_000785"
     CDS             21101..21712
                     /gene="lolA"
                     /locus_tag="ACHW42_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR8942725.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21723..23066
                     /gene="rarA"
                     /locus_tag="ACHW42_000786"
                     /gene_synonym="mgsA"
     CDS             21723..23066
                     /gene="rarA"
                     /locus_tag="ACHW42_000786"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR8942726.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23157..24449
                     /gene="serS"
                     /locus_tag="ACHW42_000787"
     CDS             23157..24449
                     /gene="serS"
                     /locus_tag="ACHW42_000787"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR8942727.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24689..27132
                     /gene="dmsA"
                     /locus_tag="ACHW42_000788"
                     /pseudo
     CDS             24689..27132
                     /gene="dmsA"
                     /locus_tag="ACHW42_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27143..27760
                     /gene="dmsB"
                     /locus_tag="ACHW42_000789"
     CDS             27143..27760
                     /gene="dmsB"
                     /locus_tag="ACHW42_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR8942728.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27762..28553
                     /locus_tag="ACHW42_000790"
     CDS             27762..28553
                     /locus_tag="ACHW42_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009390 - dimethyl sulfoxide reductase
                     complex [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFR8942729.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28589..29215)
                     /gene="ycaC"
                     /locus_tag="ACHW42_000791"
     CDS             complement(28589..29215)
                     /gene="ycaC"
                     /locus_tag="ACHW42_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR8942730.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29530..30678
                     /locus_tag="ACHW42_000792"
     CDS             29530..30678
                     /locus_tag="ACHW42_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8942731.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            30696..>30905
                     /locus_tag="ACHW42_000793"
     CDS             30696..>30905
                     /locus_tag="ACHW42_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8942732.1"
                     /translation="MNLLFHTLLFCFFYLTCFFPNFIRLKIKCAAGYNFAFRYFRTSY
                     LPRIIPRFTDAPLILAKDLSMAGNVQ"
BASE COUNT         7601 a         7603 c         8311 g         7390 t
ORIGIN      
        1 atttttcaga caattgccat gcttaaatgt gatgtcatca cgtattagca aggcctttcc
       61 cgttatactg cgagcgtaaa ggataagtca catatttctg gaggggatat gattcttgag
      121 cgcgttgaaa ttgtgggttt tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac
      181 aacgtcctga ttggggagaa cgcgtggggt aaatccagct tgctggacgc cttaaccctg
      241 ctgctatcgc cagaatcaga tctctaccat tttgagcgcg acgatttctg gttcccgccg
      301 ggagatatca acgggcgaga acatcatctg catattattt tgaccttccg cgaatcgctg
      361 ccaggccgac atcgggttcg ccgttatcgg ccgctggaag cgtgctggac gccatgcacc
      421 gatggctatc accgtatttt ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg
      481 atgacactgc gcagttttct cgataaagac ggacatccga ttgatgttga ggatattaac
      541 gatcaggcac gccatctggt gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt
      601 atgcgccgta ttcgtaacgg cacggtgcca aatgtcccca atgtggaagt caccgcgcgc
      661 cagctcgatt tcctcgcccg tgagttgtcc tcacatccgc aaaatctctc tgatggacag
      721 attcgtcagg gactttccgc aatggtgcag ttgcttgagc attatttctc tgagcagggg
      781 gctggacagg cgcgatatcg tttaatgcgg cggcgggcca gcaatgagca acgaagctgg
      841 cgctatctgg acatcatcaa ccggatgatt gaccgacctg gagggcgctc gtatcgggtt
      901 attttgctcg gcctgtttgc tactttattg caggcaaaag gcacattgcg actggataaa
      961 gacgcgcgtc cgttattgct aattgaagat ccagaaaccc gcctgcatcc gattatgctt
     1021 tccgttgcct ggcatctgtt gaatcttttg cctttacagc gtattgctac caccaactct
     1081 ggcgagctgc tttcgttaac tccagtggaa catgtttgcc gcctggtgcg tgaatcttct
     1141 cgtgttgccg cctggcgtct ggggccaagt ggcttgagca ccgaagacag ccgccgcatc
     1201 tcttttcaca ttcgttttaa tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg
     1261 gaaggggaaa cggaaacctg ggttatcaat gaactggcgc gtcagtgcgg acatcatttt
     1321 gatgccgaag ggatcaaggt cattgagttt gcccagtccg ggctaaagcc actggttaaa
     1381 tttgcccgcc gaatggggat tgaatggcat gtactggtcg atggcgatga agcagggaag
     1441 aaatatgccg ctacggtacg cagcctgttg aataacgatc gggaagccga acgagaacat
     1501 ttaacggcgt taccggcgct ggatatggaa cattttatgt atcgccaggg attttccgat
     1561 gtgttccacc gcgtggcgca aatcccggaa aatgtaccga tgaatctacg caaaattatc
     1621 tcgaaagcga tccatcgctc ttccaaaccc gatcttgcca ttgaagtggc aatggaggca
     1681 ggacgtcgtg gtgtggactc cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg
     1741 tggctggcgc gcggtcgcgc ggattaaccg cgaaacatcg tggccatttg tggctgaata
     1801 gcgtcgagca tctcatagcg ccgacggtat tcagcccgtt ttttactggc gatttcggca
     1861 atctcttttc gtgctatctg tgctggaagg cggtaatggc gttcagcatc acatacgccg
     1921 ccaaccgact cccagaacgc gttgtaatca gcatggatct tgccttcttt atcgcgataa
     1981 cgcaggctgc ggtaaatatg cgtttcattg ctgacggcaa taatctgctc tacctgcaaa
     2041 cgttgggcaa acagacaggc cgcttccatc acgaggcgtt tgggaaatag cccgtggcag
     2101 gctttcgtcg cattctggat ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg
     2161 ccaataaaca tcgttctttt cccctgatat tcacacaggg taaacgtgat ctctgccaga
     2221 ggaatacctt cgctgttgcg gaacaggatt gtgctgtcac cttctttatc cattgagatc
     2281 atcatggtca gctcaagcgt gaactgctca ccgtttttgc cttccagttt agccagttgc
     2341 agcccggggg tattcaaata taagctgaat tcttccgccg acatacaccc gcggagtaac
     2401 gcataatggt aacgtaacgc ctccagcaat tgcttacggc taagattcgc ggcaaggtaa
     2461 gggcgatgca gacgcacagg cagtcgcggc tggcgcgtta acaacacatt gagattaggc
     2521 cagtgggaaa gttcgttcat ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc
     2581 aaaaatttct ggcgaaaact acagcgatgc cagaatttac ccggccgaca ctgtccacgt
     2641 gccagactaa gaaaaagtga caggctgctg agagattcag atggcgtaaa ggtccgttca
     2701 gttagctgcg acatattcat gaaatcaatg gttatacatg acgtcgattt caccattgcg
     2761 tatcttaacc aaacatcaat agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat
     2821 ttcgttgaag ttaatgaccc ggattggcat atggagtatt cagaatattt atgaaaaagc
     2881 ggaaaaccgt gaagaagcgt tacgttattg cgctggtgat agtcatcgcc ggactgatta
     2941 cgttatggag aattcttaac gcacccgtgc cgacttatca gacactgatt gtgcgccccg
     3001 gtgatttaca gcaaagcgtg ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg
     3061 tgggcgcgca ggtcagcggt cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa
     3121 aaaaagacca gcttttaggg gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg
     3181 tcgaagcaac gctgatggag ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac
     3241 tggcgcgggt gacgtattcc cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc
     3301 aggatctcga taccgccgcg acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg
     3361 acgcgcaaat caagcgcaat caggcctctc tcgatacggc taaaaccaat ctcgattaca
     3421 cccgtatcgt tgccccgatg gctggggaag tgacgcaaat caccactctg caaggccaga
     3481 cggtgattgc cgcacagcaa gcaccgaaca ttctgacgct ggcagatatg agcactatgc
     3541 tggtaaaagc gcaggtttct gaagcggatg taatccacct gaagccgggg caaaaagcct
     3601 ggtttacggt gcttggcgat ccactgacgc gctacgaggg gcaaatcaag gatgtgctac
     3661 cgacgccgga aaaggtaaac gacgctattt tctattacgc ccgttttgaa gtacccaacc
     3721 ccaatggttt gctgcggctg gatatgaccg cgcaagtgca tattcagctc accgatgtga
     3781 aaaatgtgtt gacgatccct ctgtcggcgt taggcgatcc ggttggcgat aatcgttata
     3841 aagtcaaatt gttgcgtaat ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta
     3901 acgataccga tgttgagatt gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg
     3961 aggccaaacc aggagctgca caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag
     4021 ctatcctgcc ggtgatgagc aggttgaggt gctgaagggc atcagcctca atatttatgc
     4081 gggtgagatg gtcgcgattg ttggcgcttc gggttccggt aaatcgaccc tgatgaatat
     4141 tctcggctgc ctggataagg ccaccagcgg cacctatcgc gtagccggtc aggatattgc
     4201 cacgctggac gccgatgcgc tggcgcaact gcgccgcgag catttcggct ttattttcca
     4261 gcgttaccat ttgcttccgc atttaaccgt cgagcagaac gttgaagtac ccgccgtcta
     4321 tgccggtctt gagcggaaac agcgactgct tcgtgcccag gagttgctgc aacggctggg
     4381 gctggaagac cgtacagagt attatccggc acagctttcg ggtggtcagc aacagcgcgt
     4441 cagcatcgcg cgggcattga tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg
     4501 cgcactggac agccattctg gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg
     4561 cgggcatacg gtgattatcg tcacccacga tccgcaggtc gctgctcagg ccgagcgggt
     4621 gatcgaaatt cgcgacggcg aaattgtgcg caatcctccc gccattgaaa aagtgaatgt
     4681 tgctggcggg acggagcctg ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg
     4741 ttttaacgag gcgctgacga tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt
     4801 actgaccatg ctggggatta ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg
     4861 tgacgccgcc aaacaaatgg tgctggcgga tattcgttct attggtacga atactattga
     4921 tgtctatccc gggaaagatt ttggcgatga cgatccgcaa tatcagcagg cgctgaagta
     4981 cgacgactta atcgccatcc aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc
     5041 gcaaaacctg cgcctgcgtt ataacaatgt tgatgttgct gccagtgcca atggcgtgag
     5101 cggcgattat tttaatgtct atggcatgac cttcagtgaa ggaaacacct ttaatcagga
     5161 gcagctgaac ggtcgtgcgc aggtcgtggt tctcgacagt aatactcgcc gccagctttt
     5221 cccccataaa gcagatgtgg ttggcgaggt gattctggtc ggcaatatgc ccgccagagt
     5281 cattggtgtg gcggaagaaa aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg
     5341 gctaccttac agcacgatgt ccgggcgagt tatgggccag tcgtggctta actccattac
     5401 tgtcagggtg aaagaaggat ttgacagcgc cgaggcggaa cagcaactca cgcgtttact
     5461 ttcactgcgc cacggtaaga aggatttctt tacctggaac atggacggcg tcttgaaaac
     5521 tgttgaaaag accacacgta ctttacaact gtttctgacg ctggtggcgg tgatttcgct
     5581 ggtggtgggc ggtattggtg taatgaatat tatgctggtg tcagtgaccg agcggacgcg
     5641 ggaaattggc attcgcatgg ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct
     5701 gatcgaagcc gtactggttt gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt
     5761 aattgctttc accttgcagc ttttcttacc cggctgggag attggttttt caccgttggc
     5821 gctgctgctg gcgtttctct gctcgacggc caccgggatt ttatttggct ggttacccgc
     5881 acgaaatgcg gcacgactgg atccagtaga tgctctggca cgggagtaat ttttgagata
     5941 aaaatgccag ccgatcgggc tggcattttg cctttaggat gtacacaatg agacagaaga
     6001 gctatgcgac tgccgcttct acttcgacgg gcacaataac actggcgtga ttgccttttg
     6061 gcccctggtg gacatcaaac tgaacggatt gtccagcttt tagcgttctg taaccatcca
     6121 tctgaatggt ggaataatga gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac
     6181 caaacccttt ggcattgttg aaccacttaa cagtaccctt ttccatgctt cgacatcctt
     6241 cgcaaatctt atacaagtaa gatggaataa accggggtca gagagggggc tgttcaaaac
     6301 ctcgccaact ctagaaatac aatttagaga attagggcga gccgtcaagc atttgacagg
     6361 ggccaggggg caggtatgaa tcaaaaattt gaagcagtta acgctattga caggaatgtg
     6421 acagatgtcg ctgatgccaa cgatagatga tagttatcta tcatgtggag tagattggtc
     6481 aggcaaataa gctcttgtca gcggcagggc gttctgccga taaccgtaac cgaagatgat
     6541 aactgacaat gggtaaaacg aacgactggc tggactttga tcaactggcg gaagaaaaag
     6601 ttcgcgacgc gctaaaaccg ccatctatgt ataaagtgat attagtcaat gatgattaca
     6661 ctccgatgga gtttgttatt gacgtgttac aaaaattctt ttcttatgat gtagaacgtg
     6721 caacgcaatt gatgctcgct gttcactacc aggggaaggc catttgcgga gtctttaccg
     6781 ccgaggttgc agaaaccaaa gtggcgatgg tgaacaagta cgcgagggag aatgagcatc
     6841 cattgctgtg tacgctagaa aaagcctgaa tgcaggcata aaaattgggg gaggtgccta
     6901 tgctcaatca agaactggaa ctcagtttaa atatggcttt cgccagagcg cgcgagcacc
     6961 gtcatgagtt tatgaccgtc gagcacttgt tactggcgct gctcagtaac ccatctgccc
     7021 gggaggcgct ggaagcgtgt tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct
     7081 ttattgaaca aaccacaccc gttctgcctg ccagtgaaga ggagcgcgac acacagccga
     7141 cgctgagttt tcagcgtgta ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca
     7201 atgaggttac cggtgcaaac gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg
     7261 catatctgtt gcgtaaacat gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg
     7321 gcacgcgtaa agacgagccg acacagtctt ctgatcctgg cagccagcca aacagcgaag
     7381 aacaagctgg tggggaggaa cgtatggaga atttcacgac gaacctgaac cagcttgcgc
     7441 gtgtgggcgg aatcgaccca ctaattggtc gtgagaaaga gctggagcgt gctattcagg
     7501 ttctctgccg tcgccgtaaa aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa
     7561 ccgcgattgc agaaggtctt gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg
     7621 ctgactgtac gatttactct ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg
     7681 gcgactttga aaaacgtttt aaagcgttgc tcaagcagct ggagcaggac actaacagca
     7741 tcctgtttat tgatgagatc cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg
     7801 tcgatgcggc taacctaatc aaaccgttgc tctccagcgg taaaattcgc gtaattggtt
     7861 cgacaaccta tcaggagttc agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct
     7921 tccagaaaat tgatattact gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc
     7981 tgaaaccgaa gtatgaagcg caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg
     8041 cggtagagct ggcggtgaaa tacattaacg atcgtcatct gccggataaa gccattgatg
     8101 ttatcgacga agcaggcgct cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg
     8161 ttaatgtggc ggatattgag tccgtggtgg cccgtattgc acgcattcca gagaagagtg
     8221 tttctcagag tgaccgcgat accctgaaaa aactcagcga tcgcctgaaa atgctggtct
     8281 tcggccagga taaagccatt gaggcgctga ctgaagccat taagatggcg cgtgcaggtt
     8341 taggtcacga acataaaccg gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga
     8401 aaacagaggt gacggtacag ctttcgaaag cgttgggcat tgagcttctg cgctttgata
     8461 tgtccgagta tatggaacgc cataccgtca gccgtctgat tggtgcgcct ccgggatacg
     8521 ttggttttga tcagggcggt ttgctgaccg atgcggtaat caagcatcca catgcggtgc
     8581 tgctgctgga cgaaatcgag aaagcgcatc cggacgtgtt caatattctg ttgcaggtga
     8641 tggacaacgg tacgctgacc gataacaacg gacgcaaagc ggacttccgt aacgtggtgc
     8701 tggtgatgac cactaacgcc ggggtacggg aaactgagcg caaatccatt ggtcttatcc
     8761 accaggataa cagcaccgat gcgatggaag agatcaagaa gatctttaca ccggaattcc
     8821 gtaaccgtct cgacaacatt atctggttcg atcatctgtc aactgacgtg atccatcagg
     8881 tggtggataa attcatcgtc gagttgcagg ttcagctgga tcagaaaggt gtttctctgg
     8941 aagtgagcca ggaagcgcgt aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg
     9001 cacgtccgat ggcgcgtgtc atccaggaca acctgaaaaa accgctcgcc aacgaactgc
     9061 tgtttggttc gctggtggac ggcggtcagg tgacggttgc gctggataaa gagaaaaatg
     9121 agctgactta cggattccag agtgcacaaa agcacaaggc ggaagcagcg cattaatctg
     9181 attgtcaggt aggttggtga agtccgtaat ctcgtcaggg gttacggact ttttatttat
     9241 ggggtggaac ggtgaaaacc ctatttttgg aggtgaaggt aagttgttga taattagtgc
     9301 tgctggaagg taaggataaa aaagggtgct gcaggagaat gggatggttt tgctttatta
     9361 acaacgggct aaacgtgtag tatttgagtt cactgccgta caggcagctt agaaatcgac
     9421 ggggtgcggt aaaacctttg cgaacgcgtt cactgccgta caggcagctt agaaattcac
     9481 aggtaacata ctccacccgc ccaccatgtt cactgccgta cagacagata aaatgcgaaa
     9541 aaaaagctcg cactttcgta cgagctcttc tttaaatatg gcggtgaggg ggggattcga
     9601 acccccgata cgttgccgta tacacacttt ccaggcgtgc tccttcagcc actcggacac
     9661 ctcaccaaat tgttttgcta ccaaacctca tgggtggcaa cggggcgcta ctatagggag
     9721 ttggagtaaa acggtcaaga agaattttaa tgataattat tgtttgctca tactgtaaac
     9781 aacttgtgca gtatatctac atcgagacaa gttatggact tatacttcca aagtacttca
     9841 tacatatcac aaaataaaaa ggccggttaa accgaccttt tactcgttct ttctcttcgc
     9901 ccatcaggcg gtaaaacaat cagcgactac ggaagacaat gcggcctttg ctcaggtcgt
     9961 acggggtcag ttcaacagtc actttgtcgc ccgtcaggat gcggatgtag tttttgcgca
    10021 ttttaccgga gatgtgtgca gtaaccacgt gaccgttttc taactctacg cggaacatgg
    10081 tattaggcaa cgtttcaaga acggtacctt gcatttcaat attgtcttct ttggccatct
    10141 aatcctctgg ggtatcacta ccgtaatttg aaccggcaag ataatgccga agttctgtaa
    10201 ataagtaaag atttgcgcgc taaatcgcaa caaacaggtt cggcacatta ctccgaaaac
    10261 acacggctaa gccgcaccaa aagcgcaacg tataagggag cggtgagata aacgatgggc
    10321 gttacctgac gcgaaaaatt ccttatcggc agcggggtaa tgagcgtaac caactctgcg
    10381 accgcaatta taacactctg gggagaaatg tgccgaaaac attcattctt gtggtgaaaa
    10441 caagcaccgt ggtacccaga aattattcgg caatcgtccg aggcgcattt gattgagata
    10501 attaaggtaa tcccggcggg gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag
    10561 gacctggcag tcgataagct taccgccatg accgataaat tcctcacaga ataccagaag
    10621 cgccgtttta gacgcatttt ccatccggct gaacatggac tcgccacaaa atagcgttcc
    10681 ctgggccacg ccgtacatac cgccgacaag ctcatcttca cgccagactt caatggagtg
    10741 ggcatgaccg agttcgtgaa ggcgatggta ggcttcgacc acgccacgcg tgatccaggt
    10801 tccttcttcg cgatcgctgg cacagccttc aatgacctga ccaaaagcgt aattcatcgt
    10861 gacacgatag ggcgagcgtt tatgaaatcg cttcatacta cggctgatat gcagtgattc
    10921 tggccatagc accgcgcggg gatcgggcga ccaccagagg atggggtcgc ctggagaaaa
    10981 ccacggaaaa ataccacgct ggtaagccat taacaggcgc gcagggctaa gatcgccccc
    11041 aagtgccagc aggccgttag gctcacgtaa tgcgccttcc ggggaaggga aggctattga
    11101 atggcgagaa agctgaacca agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca
    11161 ataatagctt acaaaccctg cttgaactgg taataacgcc cctgtctggc aagcagttct
    11221 gcgtgagtac cttgctcaat aatttgcccg ttgtccatca ctattatttg ttggaaacga
    11281 gagagtccgc gaagtcgatg ggtgaccatt aacaccgttt tctcacgcat catttttgca
    11341 agcaattcaa ggatctggct ttcggttgtg gcatctaagc cttcggtagg ttcatccagc
    11401 aacaccagtg gcgcatcatg taacagcgta cgggcgatag ccagacggcg cagttcacca
    11461 ccggagagct ggcgtccgcc ttcacctaac caactgttga gacctgcatc ctcgagcagc
    11521 ttctccagac caacgcgacg caagatctcc gacagagcct catcactact gccaggcgag
    11581 gcgagtaaca gattatcacg cagtgtggcg ctaaacagat gcactcgctg aggaacaacg
    11641 ctgatggtct gtcgtagagc cgcttcattc aggctggcta tggggctatc gttaagcaaa
    11701 atctcgccct gttgcgggtc ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg
    11761 catccggttc gcccgagaat cgctatatgt tccccggcgt ttacctgaag agaaatccct
    11821 ttaagtgcct gttgggattg ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa
    11881 acgcgatcgg caacacgagt ttgggtatca gggaaggtga cctccggttt ttgatccgtt
    11941 aagtcagaga tacgtacggc agaggcaatg acttgcccca gatgctgaaa tgcacccgtt
    12001 actggtgcca gtgcttcaaa cgcggctaac gcacaaaaga caaacagggc aattaatgcg
    12061 ccgggttgag cattgccgcc aacgccgcca gacgccatcc acagcatcag gatcaccgct
    12121 aacgcgccaa tgagcagcat tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt
    12181 tgcgcttcca gccattgaat ttctgtattc tctagttgcg tgcgataacg atcgctggca
    12241 ccaaaaatgg tcagctcagc ttgcccttgc agccaggccg tcagttgttg gcgatactgt
    12301 ccgcgaagat gagtcagatt ttgcccggtg ctttttcccg cacgataaaa cagcggtggc
    12361 atcaggaaaa gcgtcagtaa cataacgctg cccagcgtaa aggcgagggt gaaatcaagg
    12421 aaacttaacc cgattgtcac caccataatc accacaaaag cgcccaccag cggcgagata
    12481 acgcgcaggt aaagatgatc gagcgtatca acatccgcca ccacgcggtt gagcaattcg
    12541 ccctgacgat agcgcgccag tccggcaggg gagaggggca acaatttgct gaaggtgtaa
    12601 atgcgcagat gctgcaacac gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa
    12661 cgcccggcag tacgggtgat tgcggcacca cgcacgcccg cagcgggtag catatagttg
    12721 aagctgtaca gtccggcaac ccccgcaacc gctgaggccg agaggaacca gccggaaagt
    12781 gtcaacagac cgatactggc gagcagcgtc acaattgcca gcacaatacc aagacttaac
    12841 atccatttat gacgtttata cagtgccaga tagggtagca aagcgcgcat ttaaatctcc
    12901 tcctgacgat gggccagtaa tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa
    12961 cgtccttgct caataatccg gccatcctgc ataacccaaa tgacatccca gtcagcaaga
    13021 tcttctaact ggtgggtgac cattaacgtt gtctggcgca gagaggcggc attcagcgcc
    13081 tccattacgc gctgttcact gtgagcatca aggctggcag cgggttcatc caacagtaat
    13141 agcgaacagg gatttagtaa cgcacgggcc accgccacgc gctgcgcctg ccccacggaa
    13201 aggcgggcag cctggtcgcc aacaggtgta tcaacgcctt gtggcaggag cggtagaaac
    13261 tcgctgaccc aggcgttatc cagcgctgct tgtaattctt gttcgctggc atcaggtcgc
    13321 gccagtagta cgttatcccg caattttgct gccggtaatt gtgggttttg cccaacccag
    13381 gagagatgtt tacgccatga ttctggtgat aaatcacgta attctatccc gttgattcgt
    13441 aatgatccct gatatgagag aaaaccagaa agcgcgttca gcagtgagct tttacctgaa
    13501 ccgctgcggc caaccaacac tgcacgttgg cctgctggca aagtaaagtt cagcggtccg
    13561 gccagcgttt taccttccgg cgacgtgata aacagatcct cggcctcaat ggtcagcgga
    13621 tcggtcaatg ctaattccgc ctcaccgcgc tgcggatggg cgagcggggt ttccataaac
    13681 gttttcagac tgtcagctgc accaacagcc tgggctttag catgataaaa cgtaccgaga
    13741 tcgcgtaatg gctggaaaaa ctctggcgca aggatcaggg ccagaaaacc tgcagccagc
    13801 gtcaccccag tatcgtagtg accaaaatcc agctcgccga gataggaaaa accaaagtag
    13861 accgccacca gagcaattga cagcgaggta aaaaattcga gaatgccgga ggataaaaac
    13921 gccagtcgta gcacttccat tgtccgttgg cggaaatctt ccgaagcaga acgaatactt
    13981 tcaatttcag cttcaccacg accaaaaata cgcaatgttt ccatgccgcg caggcgatcg
    14041 aggaaatgcc cacttaagcg agcaagagcg agaaagttac gccggttagc atcggcagcc
    14101 cccattccaa ccagcgccat aaacaacgga attagcggtg ctgtgcccag cagaatgagc
    14161 gccgcagccc agttagaggg gaagatcgcc accacaatca gcaacggcac cgacactgcc
    14221 agcgccattt gcggcagata gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt
    14281 accagcgtcg cccagctccc cgcaggttta ccctgaatcc acgctggccc tgcttgttgc
    14341 agacggtcga gaacctgacg gcggatggca aagcggatat gctgcccggc gtgataaccc
    14401 acccgttcgc gtaaccagac cacccatgcg cgcaatacaa aggtcagaac cagtaacgta
    14461 aagggaagca gcagggcttc acggggaata ttctccataa tcatatgttg cagaattcgc
    14521 gccatgaacc aggcctgggc aatgatcaat atgccgctta caaagcccag cagacgagaa
    14581 atattcagcc aacgttggga gatgacgctt tgctgtttta accagcgggt taactctttt
    14641 tgacgagatt tattcattgc acgcttagca ggtgagttat cagaattatt tgcagagcaa
    14701 tgttacaacg gggaaaaaat aaaggcgacc catagtcgca aggtgtcgcc ttctttactt
    14761 ttgttactga tttgtaaaat tattttgcgt cagctaaacc atcgaggtag cgttccgcat
    14821 caagtgctgc catgcagcca gtaccggccg aagtaatggc ctggcgataa atgtgatcca
    14881 tcacgtcgcc tgcggcaaag acgccaggaa tgctggtctg ggtggcatta ccatgaatac
    14941 ccgactgtac tttgatgtag ccgttttcca gttccagctg cccttcgaaa atcgcagtat
    15001 tcgggctgtg accgatagca acaaacagac cggcaacgtc gagtgactcg atgttatcgc
    15061 tgttttgcgt atcgcgcaga cgaacgccag tgacacccat ttgatcgccg gtcacttctt
    15121 ccagcgtacg gttggtgtgc agaatgatgt tgccgttctc cactttatcc atcagacgct
    15181 taataaggat tttttccgcg cggaaaccgt cacggcggtg aatcagatgc acttccgaag
    15241 cgatgttaga cagatacagc gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa
    15301 ctttctggtt gcgatagaag aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt
    15361 taaaggcttc ttcagagggc aggccgagat agcgtgcaga agctccggtg gcaataatca
    15421 gcgcgtcgca agtgtattcg ccgttatcgc cattcagacg gaacggacgg ttttgcagat
    15481 ccaccttgtt gatatgatca aaaatgatct cagtttcaaa cttggtggca tgttcgtgca
    15541 tgcgcgccat taataacgga ccggtcagat cgtttggatc accaggccag ttttccactt
    15601 ccgtggtggt ggtcagttgg ccgccttttt ccatgccggt aatcagcaca ggttgcaggt
    15661 tggcgcgcgc cgcgtagaca gcagcggtgt atcccgccgg gcctgaaccc aggataagca
    15721 gtttactgtg tttggtcgtg cccatgagat ccccatagtt gttggcagac aatgggcagg
    15781 attgtaggga atttacagac gtaaaaaaag agtatgacga ttttgttaac aatttgtgca
    15841 atcggcagca tcgataagca ggtcaaattc tcccgtcatt atcacctctg ctacttaaat
    15901 ttcccgcttt ataagccgat taaatgatga ataaacgccc ctgttaatga atatctggca
    15961 tgttgtacta aaaatcgatg ttttgctttg acaatcccct ggtgttttgc gaaaacattc
    16021 gaggaagaaa aaaacagtat tcttatatgc gcataaccat gtatgtaaat accatgttta
    16081 ccgtgctagt gaaatctacg tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc
    16141 cacaaggcaa cggtcttctc accgtagacc caggcattgc gcgccgtgaa tcttcatgat
    16201 ttcggtctat cgtgacgggt agcgactctg aacagtgatg tttcagggtc agacaggagt
    16261 agggaaggaa tacagagaga caataataat ggtagatagc aagaagcgcc ctggcaaaga
    16321 tctcgaccgt atcgatcgta acattcttaa tgagttgcaa aaggatgggc gtatttctaa
    16381 cgtcgagctt tctaaacgtg tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg
    16441 gctggaaaga caagggttta ttcagggcta tacggcgctg cttaaccccc attatctgga
    16501 tgcatcactt ctggtattcg ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga
    16561 acaattcaat accgctgtac aaaaacttga agaaattcag gagtgtcatt tagtatctgg
    16621 tgatttcgac tacctgttga aaacacgcgt gccggatatg tcagcttacc gtaagttgct
    16681 gggggaaacc ctgctgcgtc tgcctggcgt caatgacaca cggacatacg ttgttatgga
    16741 agaagtcaag cagagtaatc gtctggttat taagacgcgc taacacggaa caggtgcaaa
    16801 atcggcgtat tttgattaca ctcctgttaa tccatacagc aacagtactg gggtaacctg
    16861 gtactgttgt ccgttttagc atcgggcagg aaaagcctgt aacctggaga gcctttcttg
    16921 agccaggaat acactgaaga caaagaagtc acattgacaa agttaagtag cggccgccgc
    16981 cttctggagg cattgctgat ccttattgtc ctgtttgccg tctggttgat ggctgcctta
    17041 ctaagcttta acccttcgga ccccagctgg tcgcaaacgg cctggcatga acctatccat
    17101 aatttaggtg ggatgcccgg tgcgtggttg gcagatacgc tgttctttat ttttggcgtg
    17161 atggcttaca ccattcccgt cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag
    17221 tccagcgacg aatacattga ttattttgcc gtttcgctac gcatcattgg cgttttggcg
    17281 ctcatcctta cctcctgtgg tctggcggca atcaacgctg acgatatctg gtattttgcc
    17341 tccggtggcg tcattggcag cttactaagc actacgctac aaccactgct acacagtagc
    17401 gggggaacta ttgcgctgct ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg
    17461 tcatgggtga ccattgctga aaaactcggc ggctggattt taaacattct caccttcgcc
    17521 agtaatcgta cccgtcgcga tgatacctgg gtcgatgaag atgagtatga agacgacgaa
    17581 gagtatgaag atgaaaatca cggcaaacag catgaatcac gccgtgcccg tattcttcgc
    17641 ggcgcgctag cgcgtcgtaa acggttggcg gaaaaattca ttaatccgat ggggcggcaa
    17701 acagacgctg cgttgttctc cggtaagcgg atggatgatg acgaagagat tacctacact
    17761 gcacgcggtg tggctgccga cccggacgac gtcctatttt cgggcaatcg tgcaacgcag
    17821 ccagaatatg acgaatacga tccattatta aacagtgcgc caattaccga acctgtcgct
    17881 gtagcagctg ctgctaccac ggcgacacaa agctgggctg cgccggttga acctgtgact
    17941 cagacgccgc ctgttgcctc tgttgatgtt ccaccttcgc aacctacagt agcctggcag
    18001 cctgtaccgg gtccacaaac gggagagccg gttattgctc ctgcaccgga aggttaccca
    18061 cagcagtcac aatatgcgca gcctgcagtg caatataatg agccgctgca acaaccagta
    18121 cagccgcagc agccgtatta tgcacctgca gctgaacaac ctgcgcaaca gccgtattat
    18181 gcccctgcgc cagaacaacc ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc
    18241 acttttgctc cacagtctac ataccagact gagcaaactt atcagcagcc agccgctcag
    18301 gagccgttgt accaacagcc gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc
    18361 gttgtagaag agacaaaacc cgcgcgtccg ccgctttact attttgaaga agtggaagag
    18421 aagcgagccc gtgaacgtga acaacttgcg gcctggtatc aaccgattcc agaaccggtt
    18481 aaagaaccag aaccgatcaa atcttcgctg aaagcacctt ctgttgcagc agtacctcca
    18541 gtagaagccg ctgccgctgt ttctccgctg gcatctggcg tgaaaaaagc gacactggcg
    18601 acgggggctg ccgcaaccgt tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg
    18661 cgtccgcagg tcaaagaggg gattggcccg cagttgccac gaccgaaacg tatccgcgtg
    18721 ccaactcgtc gtgaattggc gtcttacggt attaagctgc cctcacagcg tgcggcggaa
    18781 gaaaaagccc gtgaagccca gcgcaatcag tatgattctg gcgatcagta taacgatgat
    18841 gaaatcgatg cgatgcagca ggatgaactg gcacgtcagt tcgcccagac acagcagcaa
    18901 cgctatggcg aacagtatca acatgatgtg cccgtaaacg cagaagatgc agatgctgcg
    18961 gcagaggctg aactggctcg tcagtttgcg caaactcaac aacaacgtta ttccggcgaa
    19021 caaccggctg gggcgaatcc gttctcgctg gatgattttg aattttcgcc aatgaaagcg
    19081 ttgctggatg atggtccaca cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag
    19141 ccgcaacaac cggttgcacc acagcagcag tatcagcagc cacaacagcc ggttgcgcca
    19201 cagcagcagt atcagcagcc acaacagcag gttgcgccgc agccacaata tcagcagccg
    19261 caacaaccgg ttgcgccaca gcagcaatat cagcagccgc aacaaccagt tgcgccgcag
    19321 ccgcagtatc aacagccgca acaaccggtt gcaccgcagc cgcagtatca gcagccacaa
    19381 cagccagttg cgccacagcc gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc
    19441 gacagccgtc cgttgcacaa accgacgacg ccactgcctt ctctggattt gctgacaccg
    19501 ccgccgagcg aagtggagcc ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg
    19561 gaagcgcgtc tggctgattt ccgtattaaa gccgatgtcg ttaattactc tccggggcca
    19621 gttatcactc gctttgaact taacctggct cctggcgtaa aagcggcgcg catttctaac
    19681 ttgtcacggg accttgcccg ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt
    19741 cctggcaaac cctatgtagg tctggagtta ccaaataaaa aacgacaaac cgtttatctg
    19801 cgcgaagttt tggataacgc caaattccgc gataatccgt cgccattaac cgtggtgctg
    19861 ggtaaagata tcgccggtga gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg
    19921 gttgcgggga ctaccggttc cggtaaatct gtcggtgtga acgcgatgat cctgagcatg
    19981 ctttataaag cacagccaga agatgtgcgt ttcatcatga tcgacccgaa aatgctggag
    20041 ctttcggttt atgaaggcat tccgcacctg ttaacggaag tcgttactga tatgaaagat
    20101 gccgccaacg cgctgcgctg gtgtgttaac gagatggagc gtcggtataa actgatgtct
    20161 gcgctgggtg tgcgtaatct ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg
    20221 atgcgtccga ttccagaccc gtactggaag ccgggtgaca gtatggatgc ccagcatccg
    20281 gtgctgaaaa aagaaccata cattgtcgtg ttggttgacg aatttgccga cctgatgatg
    20341 acggtaggta aaaaagtgga agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg
    20401 ggtatccacc tcgtactggc aactcagcgt ccatcggttg atgttattac tggtctgatt
    20461 aaagcgaata ttccgacccg tatcgccttt accgtatcca gtaagattga ctcacgtacc
    20521 attcttgatc aggctggcgc ggaatcactg ctgggtatgg gggatatgct ctactctggg
    20581 ccgaactcca cgttgccggt acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat
    20641 gccgtggtgc aggactggaa agcgcgtggt cgcccacagt atgttgatgg catcacctcc
    20701 gacagcgaaa gcgaaggtgg tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg
    20761 ttcgatcagg cggtgcagtt tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag
    20821 cgtcagttcc gcattggtta taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag
    20881 gggattgtca gcgaacaggg gcacaacggt aatcgtgaag tgctggcccc accgccgttt
    20941 gactaattaa tgcattgccg gataaggcgc ggtagcgtcg catccggcac tctatcaact
    21001 gaaaattcag tattttcttc tttcctcaag ctgattatta gcctggaata gagagtagag
    21061 ggaactcccg atcgggagtg acgtaatttg aggaataatg atgaaaaaaa ttgctatcac
    21121 ctgtgcatta ctctcaagct tagtagcaag cagcgtttgg gctgatgccg caagcgatct
    21181 gaaaagccgc ctggataaag tcagcagctt ccacgccagc ttcacacaaa aagtgactga
    21241 cggtagcggc gcggcggtgc aggaaggtca gggcgatctg tgggtgaaac gtccaaactt
    21301 attcaactgg catatgacac aacctgatga aagcattctg gtttctgacg gtaaaacact
    21361 gtggttctat aacccgttcg ttgagcaagc tacggcaacc tggctgaaag atgccaccgg
    21421 taatacgccg tttatgctga ttgcccgcaa ccagtccagc gactggcagc agtacaatat
    21481 caaacagaat ggcgatgact ttgtcctgac gccgaaagcc agcaatggca atctgaagca
    21541 gttcaccatt aacgtgggac gtgatggcac aatccatcag tttagcgcgg tggagcagga
    21601 cgatcagcgc agcagttatc agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa
    21661 atttaccttc accccgccgc aaggcgtcac ggtagatgat caacgtaagt agaggcacct
    21721 gagtgagcaa tctgtcgctc gatttttcgg ataatacttt tcaacctctg gccgcgcgta
    21781 tgcggccaga aaatttagca cagtatatcg gtcagcaaca tttgctggct gcggggaagc
    21841 cgttgccgcg cgctatcgaa gccgggcatt tgcattctat gatcctctgg gggccaccgg
    21901 gtaccggcaa aacaaccctc gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac
    21961 gtatttctgc cgtcacctct ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc
    22021 aaaaccgcaa tgcaggtcgc cgcactattc tttttgttga cgaagttcac cgtttcaaca
    22081 aaagccagca ggatgcattt ctgccacata ttgaagacgg caccatcact tttattggcg
    22141 caaccactga aaacccgtcg tttgagctta attcggcact gctttcccgt gcccgtgttt
    22201 atctgttgaa atccctgagt acagaggata ttgagcaagt actaactcag gcgatggaag
    22261 acaaaacccg tggctatggt ggtcaggata ttgttctgcc agatgataca cgacgtgcca
    22321 ttgcagaact ggtgaatggc gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg
    22381 atatggccga agtctatgat agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa
    22441 tcgccggtga acgtagcgcc cgctttgata acaaaggcga tcgcttttac gatctaattt
    22501 ccgcactgca taagtcggta cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc
    22561 gaattattac cgctggtggc gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt
    22621 ctgaagacgt cggtaatgcc gatccacggg cgatgcaggt ggcaattgcg gcctgggatt
    22681 gctttactcg cgttggcccg gcggaaggtg aacgcgccat tgctcaggcg attgtttacc
    22741 tggcctgcgc gccaaaaagc aacgctgtct acactgcgtt taaagccgcg ctggccgatg
    22801 ctcgcgaacg cccggattat gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa
    22861 tgaaggaaat gggctacggg caggaatatc gttatgctca tgatgaagca aacgcttatg
    22921 ctgccggtga ggtttacttc ccgccggaaa tagcacaaac acgctattat ttcccgacaa
    22981 acaggggcct tgaaggcaag attggtgaaa agctcgcctg gctggctgaa caggatcaaa
    23041 atagccccat aaaacgctac cgttaatgtt atcgttgcgg taatgttgtt actgtatccc
    23101 tgtggtcgca ggctgtggcc acatctccca tttaattcga taagcacagg ataagcatgc
    23161 tcgatcccaa tctgctgcgt aatgagccag acgcagtcgc tgaaaaactg gcacgccggg
    23221 gctttaagct ggatgtagat aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg
    23281 tcaaaacgga aaacctgcaa gcggagcgta actcccgatc gaaatccatt ggccaggcga
    23341 aagcgcgcgg ggaagatatc gagcctttac gtctggaagt gaacaaactg ggcgaagagc
    23401 tggatgcagc aaaagccgag ctggatgctt tacaggctga aattcgcgat atcgcgctga
    23461 ccatccctaa cctgcctgca gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg
    23521 aagtcagccg ctggggtacc ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc
    23581 tgggtgaaat gcactctggc ctcgactttg cagctgcagt taagctgact ggttcccgct
    23641 ttgtggtaat gaaagggcag attgctcgca tgcaccgcgc actgtcgcag tttatgctgg
    23701 atctgcatac cgaacagcat ggctacagtg agaactatgt tccgtacctg gttaaccagg
    23761 acacgctgta cggtacgggt cagctgccga aatttgctgg cgatctgttc catactcgtc
    23821 cgctggaaga agaagcagac accagtaact atgcgctgat cccaacggca gaagttccgc
    23881 tgactaacct ggtgcgcggt gaaatcatcg atgaagatga tctgccaatt aagatgaccg
    23941 cccacacccc atgcttccgt tctgaagccg gttcatatgg tcgtgacacc cgtggtctga
    24001 tccgtatgca ccagttcgac aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa
    24061 tggcggcgct ggaagagatg actggtcatg cagaaaaagt cctgcagttg ctgggcctgc
    24121 cgtaccgtaa aatcatcctt tgcactggcg acatgggctt tggcgcttgc aaaacttacg
    24181 acctggaagt atggatcccg gcacagaaca cctaccgtga gatctcttcc tgctccaacg
    24241 tttgggattt ccaggcacgt cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa
    24301 cccgtctggt tcataccctg aacggttctg gtctggctgt tggtcgtacg ctggttgcag
    24361 taatggaaaa ctatcagcag gctgatggtc gtattgaagt accagaagtt ctgcgtccgt
    24421 atatgaacgg actggaatat attggctaat acccaatttt tctgaatcta aaaagcgcct
    24481 ttagggcgct ttttttgtct ccctttgata ccgaacaata attactcctc acttacacgt
    24541 aatactactt tcgagtgaaa atctacctat ctctttgatt ttcaaattat tctatgtata
    24601 caagcctata tagcgagctg ctatagaaat aattacacaa tacggtttgt tactggaatc
    24661 aatcgtgatc aagcttgagt gagccattat gaaaacgaaa atccctgatg cggtattggc
    24721 tgctgaggag agtcgccgtg gtttggtaaa aacgacagcg atcggcggcc tggcaatggc
    24781 cagcagcgca ttaacattac cttttagtcg gattgcgcac gctgtcgata gcgccattcc
    24841 aacaaaatca gacgaaaagg ttatctggag cgcctgtaca gttaactgtg gtagtcgctg
    24901 cccgctacgt atgcacgtcg tggacggtga aatcaaatat gtcgaaacga caataccggc
    24961 gatgacaatt acgacggcct acactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt
    25021 caccgtgtct acaatccgga ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt
    25081 gaaggcaaat tcgagcgcat tagctgggaa gaagcctacg acatcatcgc gaccaatatg
    25141 cagcgcctga tcaaagagta cggcaacgag tctatctatc tgaactatgg caccggtacg
    25201 ctgggcggca ccatgacccg ctcctggccg ccgggaaata ccctggtcgc gcgcctgatg
    25261 aactgctgcg gcggttatct taaccattac ggtgactact cttccgcgca aattgcggaa
    25321 ggtttgaacc atacctacgg cggctgggca gatggtaaca gcccgtcgga tatcgaaaac
    25381 agtaaattgg tagtgctgtt tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg
    25441 gtgacttact atcttgaaca ggcacgccag aaatctaatg ctcgcatgat catcatcgat
    25501 ccgcgctata ccgacaccgg tgccgggcgc gaagatgagt ggatccctat tcgtccagga
    25561 acagatgccg cactggttaa cggtctggcg tacgtcatga tcactaaaaa cctggtggat
    25621 caggcattcc tcgataaata ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg
    25681 ccgaaaaatg gtcattataa agcttatatt ctgggtgaag ggccagatgg cgtggctaaa
    25741 acaccggaat gggcctcgca aatcaccggt gttccggcag acaaaatcat caaactggct
    25801 cgtgaaatcg gcagtaccaa accggcgttt atcagccagg gatggggccc gcagcgtcac
    25861 gctaacggtg aaatcgcaac ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt
    25921 ggtattaatg gagggaacag cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt
    25981 atgccgacct tggaaaaccc gatccagacc agcatttcga tgtttatgtg gaccgatgcc
    26041 attgaacgtg gcccggaaat gacggcgctg cgtgatggtg tgcgcgggaa agataagctg
    26101 gatgtgccga tcaaaatgat ctggaactat gccggtaact gcctgattaa ccagcattct
    26161 gaaatcaacc gtacccatga aatcctccag gatgataaga agtgcgagct gattgtggtt
    26221 atcgactgcc acatgacctc atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc
    26281 gcttccgaac agatggactt tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt
    26341 ttcaacgatc aggtgattaa accgcgcttt gaatgtaaga ccatctatga aatgaccagc
    26401 gaactggcaa aacgtcttgg cgttgagcaa cagtttactg aaggccgtac ccaggaagag
    26461 tggatgcggc atctgtatgc ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa
    26521 gagttccgca agcaggggat ctttaaaaag cgcgacccac aagggcatca cgttgcttat
    26581 aaagccttcc gtgaagatcc gcaggcaaac ccactgacta cgccatcggg caaaattgag
    26641 atttattcgc aggcgctggc tgacattgcc gctacctggg aattgccaga aggcgatgtg
    26701 atcgatccac tgccgatcta cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa
    26761 cagtatccgc tgcagcttac aggtttccac tataaatctc gcgttcactc aacttacggc
    26821 aacgttgatg tgctgaaagc ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc
    26881 caaaaacgcg gtatccacaa cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt
    26941 catattgagg cgaaagtgac gccacgaatg atgccgggcg tggtcgcact gggtgaaggc
    27001 gcctggtatg acccggatgc aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc
    27061 actcaacgtc cgtctcctct cgctaagggg aatccgtcac atacaaacct tgttcaggtt
    27121 gaaaaggtgt aaggagtaac cgatgacaac ccagtatgga ttttttattg attccagccg
    27181 ttgcaccggt tgcaaaacct gcgagctggc ttgtaaagac tacaaagatt tgacgccaga
    27241 agtcagtttc cgccgcattt atgaatatgc tggcggcgac tggcaggaag ataacggtgt
    27301 ctggcaccag aacgtgtttg cctactatct gtcgatttcg tgcaaccact gcgaagatcc
    27361 ggcttgtact aaagtctgcc cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt
    27421 ggtcgatgaa gatgtatgca ttggctgccg ttactgtcat atggcttgcc cgtatggcgc
    27481 accgcaatat aacgaaacga aaggccatat gaccaaatgc gatggttgtt atgaccgtgt
    27541 tgcggagggt aaaaagccga tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg
    27601 ccctatcgac gagctgcgta aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg
    27661 agctcacttt accaaaccga atattgtgat caaacccaat gccaatagcc gcccgaccgg
    27721 ggataccact ggctatctgg caaacccgaa ggaggtgtga gatgggaagt ggatggcatg
    27781 aatggccgct gatgatcttc acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc
    27841 tggctttggc gctgctcaaa ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct
    27901 gcatgtttgg cttatgggta ctgatgggca ttggctttat cgcctctatg ctccatcttg
    27961 gttcaccgat gcgcgccttt aactcgctca accgggtagg ggcttcagca ctcagtaacg
    28021 aaatcgccag cggttcgatc ttttttgctg taggcggcat cggctggttg ctggcaatgc
    28081 tgaaaaagct gtcaccggca ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg
    28141 tcgtctttgt ctggatgatg gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt
    28201 acagcatctg gacgccgatg ggctgggcga tgcgtctgct gccagccatt tctgtactgg
    28261 cactggtggt gagtggcgtg atgtcagtga tgcagggcac agagctggca actattcata
    28321 gttctgtgca gcaggccgca gcgctggtgc cggactatgg tgcgctgatg tcctggcgga
    28381 tcgtgctttt ggccgttgcc ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc
    28441 ctgcggttcc gctactatcc gtctcattca ttctgcttct ggcaggggaa ttaatcggtc
    28501 gcggcgtatt ctatggtttg catatgaccg tggggatggc cgtcgcaagc taatcagtaa
    28561 caaccggggt ttcggccccg gttctctttt atttctgctt cgttaacgtg tcataactgg
    28621 tcatcaaatt acgataatca ggaatatggt tagagaacaa cgtcgccagt ccttcaatat
    28681 cattacgcca gtcacggtgc agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac
    28741 cagcttgcga catgcgatcc cacgccgaat gccgggtaat ttcattaaag gtccctgatg
    28801 catcagtcac gacaaagaca tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca
    28861 cgcaaacttc ggttaccaca ccggcaataa ttaactgttt tttacctgtc gctttgactg
    28921 cttttacaaa atcttcgtta tcccaggcgt taatattgcc cgggcgagca atgtaaggtg
    28981 catcggggaa ttgtgctttc agttctagaa ctaatgggcc gttaggaccg gtttcaaaac
    29041 tggtggtaag aatagttggt aagttgaaat acttagccag gtcacccagc gccagcacgt
    29101 tatttttaaa cttatcgggt tcgatatccc gtacaaggga aagtaaacca gcctggtgat
    29161 caacaagcaa aacggcagca tcatttttat caagacgaac atacggtttg gtcatcctct
    29221 tctcctttcg agagtcagtg gtacgtctga aatcggtcag acgcgatgca ttgctctgaa
    29281 agcatagacg ggaaatatga gtttgctgtg accatgaaat ttttcgactg aacgaagcgt
    29341 atgaaatttg tgttagatca ataaaaacaa tcagatgaga ttaatcatct cttcagcatt
    29401 agcgttatga atgagtaata taaatcgata ataccgccgc tgcgatatgc gcgtattttc
    29461 acccattgac aatgtttttg gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg
    29521 ttttaattca tgtccacgta tacccggcct gtcatgcttt tgctgtctgg cctgcttttg
    29581 ttgactctgg cgatcgcggt gttaaataca ctcgtgccgc tttggctcgc ccaggaacac
    29641 atgtccacat ggcaggtagg cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt
    29701 acattgctga cagggtatgt cattaaacgc attggcttta accgcagcta ttatctgtcc
    29761 tccttcattt ttgctgctgg ctgtgccggc cttggcctga tgattggttt ctggagctgg
    29821 ttggcgtggc gttttgtcgc gggcgtcggc tgtgccatga tttgggtggt tgttgagagc
    29881 gcgctgatgt gcagtgggac gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg
    29941 gtttattacg tgggaacgtt tttaggccag ttactggtca gcaaagtctc aaccgagctg
    30001 atgagcgtat tgccgtgggt tacaggtttg acattggcag ggatcttacc gctgttgttt
    30061 acgcgtgtcc tgaatcagca ggctgaaaac catgattcga cgtcaattac ggcaatgcta
    30121 aaactccgtc aggcgcggct tggcgtgaat ggctgcatta tctcaggaat cgttctggga
    30181 tctctatatg gcctgatgcc tctgtacctc aatcacaaag gggtgagcaa tgccagtatt
    30241 ggtttctgga tggcggtact ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt
    30301 ctggcggata agtttggtcg actgctggtt ttgcgtgttc aggtctttgt cgtcattctc
    30361 ggcagtattg cgatgcttag ccaggcggcg atggccccag cgttattcat cctcggtgcc
    30421 gctggcttta cgctatatcc ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat
    30481 caactggtgg cgatgaacca agccttactg ttgagctata ccgtgggaag tctgcttggc
    30541 ccgtcattta gcgctatgct aatgcagaat ttctccgata atttattgtt tatcatgatc
    30601 gccagcgtat cgtttatcta tttgctgatg ctgcttcgca acgccggtca tacgccgaaa
    30661 cccgttgctc acgtgtaaat gaattcaagc aaagtgtgaa cttactgttt cacactctgc
    30721 ttttttgttt cttctatctg acttgctttt ttccaaattt tattcgttta aaaataaaat
    30781 gtgcagcagg ttataatttt gcatttcgct atttccgcac ttcttatttg ccgcgcataa
    30841 tccctcgttt tacagatgcc cctttaattt tggcgaagga tttgtctatg gctgggaatg
    30901 ttcag
//
LOCUS       DAZSNM010000019        30872 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZSNM010000019 DAZSNM010000000
VERSION     DAZSNM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30872
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.019"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(2..298)
                     /locus_tag="ACHW42_000794"
     CDS             complement(2..298)
                     /locus_tag="ACHW42_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR8942733.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            513..1241
                     /gene="tonB"
                     /locus_tag="ACHW42_000795"
     CDS             513..1241
                     /gene="tonB"
                     /locus_tag="ACHW42_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR8942734.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="ACHW42_000796"
     CDS             complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="ACHW42_000796"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR8942735.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1784..2323)
                     /locus_tag="ACHW42_000797"
     CDS             complement(1784..2323)
                     /locus_tag="ACHW42_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR8942736.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2353..3096)
                     /locus_tag="ACHW42_000798"
     CDS             complement(2353..3096)
                     /locus_tag="ACHW42_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR8942737.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3124..3213)
                     /locus_tag="ACHW42_000799"
                     /pseudo
     CDS             complement(3124..3213)
                     /locus_tag="ACHW42_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3453..4091
                     /gene="ompW"
                     /locus_tag="ACHW42_000800"
     CDS             3453..4091
                     /gene="ompW"
                     /locus_tag="ACHW42_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR8942738.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4151..4657)
                     /locus_tag="ACHW42_000801"
     CDS             complement(4151..4657)
                     /locus_tag="ACHW42_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR8942739.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4703..5203)
                     /locus_tag="ACHW42_000802"
     CDS             complement(4703..5203)
                     /locus_tag="ACHW42_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR8942740.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5289..5468)
                     /locus_tag="ACHW42_000803"
     CDS             complement(5289..5468)
                     /locus_tag="ACHW42_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR8942741.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="ACHW42_000804"
     CDS             complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="ACHW42_000804"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR8942742.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="ACHW42_000805"
     CDS             complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="ACHW42_000805"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR8942743.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7859..9220)
                     /gene="trpCF"
                     /locus_tag="ACHW42_000806"
     CDS             complement(7859..9220)
                     /gene="trpCF"
                     /locus_tag="ACHW42_000806"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR8942744.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(9221..10816)
                     /gene="trpD"
                     /locus_tag="ACHW42_000807"
     CDS             complement(9221..10816)
                     /gene="trpD"
                     /locus_tag="ACHW42_000807"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR8942745.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(10816..12378)
                     /gene="trpE"
                     /locus_tag="ACHW42_000808"
     CDS             complement(10816..12378)
                     /gene="trpE"
                     /locus_tag="ACHW42_000808"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR8942746.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(12400..12493)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(12470..12514)
                     /gene="trpL"
                     /locus_tag="ACHW42_000809"
     CDS             complement(12470..12514)
                     /gene="trpL"
                     /locus_tag="ACHW42_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR8942747.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            12652..13533
                     /gene="rnm"
                     /locus_tag="ACHW42_000810"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             12652..13533
                     /gene="rnm"
                     /locus_tag="ACHW42_000810"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR8942748.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            13530..14150
                     /locus_tag="ACHW42_000811"
     CDS             13530..14150
                     /locus_tag="ACHW42_000811"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR8942749.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            14251..15123
                     /gene="rluB"
                     /locus_tag="ACHW42_000812"
     CDS             14251..15123
                     /gene="rluB"
                     /locus_tag="ACHW42_000812"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR8942750.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(15163..15753)
                     /gene="cobO"
                     /locus_tag="ACHW42_000813"
     CDS             complement(15163..15753)
                     /gene="cobO"
                     /locus_tag="ACHW42_000813"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR8942751.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(15750..16508)
                     /locus_tag="ACHW42_000814"
     CDS             complement(15750..16508)
                     /locus_tag="ACHW42_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR8942752.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            16728..17777
                     /gene="sohB"
                     /locus_tag="ACHW42_000815"
     CDS             16728..17777
                     /gene="sohB"
                     /locus_tag="ACHW42_000815"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR8942753.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(17813..18064)
                     /locus_tag="ACHW42_000816"
     CDS             complement(17813..18064)
                     /locus_tag="ACHW42_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR8942754.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            18444..21047
                     /gene="topA"
                     /locus_tag="ACHW42_000817"
     CDS             18444..21047
                     /gene="topA"
                     /locus_tag="ACHW42_000817"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR8942755.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKNNL"
     gene            21250..22224
                     /gene="cysB"
                     /locus_tag="ACHW42_000818"
     CDS             21250..22224
                     /gene="cysB"
                     /locus_tag="ACHW42_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR8942756.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            22543..22683
                     /locus_tag="ACHW42_000819"
     CDS             22543..22683
                     /locus_tag="ACHW42_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR8942757.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            22686..22853
                     /gene="yciX"
                     /locus_tag="ACHW42_000820"
     CDS             22686..22853
                     /gene="yciX"
                     /locus_tag="ACHW42_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR8942758.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            22967..23062
                     /gene="ymiC"
                     /locus_tag="ACHW42_000821"
     CDS             22967..23062
                     /gene="ymiC"
                     /locus_tag="ACHW42_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR8942759.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            23226..25901
                     /gene="acnA"
                     /locus_tag="ACHW42_000822"
     CDS             23226..25901
                     /gene="acnA"
                     /locus_tag="ACHW42_000822"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR8942760.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(25965..26555)
                     /gene="ribA"
                     /locus_tag="ACHW42_000823"
     CDS             complement(25965..26555)
                     /gene="ribA"
                     /locus_tag="ACHW42_000823"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR8942761.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            26725..27489
                     /gene="pgpB"
                     /locus_tag="ACHW42_000824"
     CDS             26725..27489
                     /gene="pgpB"
                     /locus_tag="ACHW42_000824"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR8942762.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            27638..27946
                     /gene="lapA"
                     /locus_tag="ACHW42_000825"
     CDS             27638..27946
                     /gene="lapA"
                     /locus_tag="ACHW42_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR8942763.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            27953..29122
                     /gene="lapB"
                     /locus_tag="ACHW42_000826"
     CDS             27953..29122
                     /gene="lapB"
                     /locus_tag="ACHW42_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR8942764.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            29315..30052
                     /gene="pyrF"
                     /locus_tag="ACHW42_000827"
     CDS             29315..30052
                     /gene="pyrF"
                     /locus_tag="ACHW42_000827"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR8942765.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            30052..30378
                     /gene="yciH"
                     /locus_tag="ACHW42_000828"
     CDS             30052..30378
                     /gene="yciH"
                     /locus_tag="ACHW42_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR8942766.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(30504..30722)
                     /gene="osmB"
                     /locus_tag="ACHW42_000829"
     CDS             complement(30504..30722)
                     /gene="osmB"
                     /locus_tag="ACHW42_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR8942767.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         7736 a         7527 c         7967 g         7642 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tagtttttct actgaaatga ttatgacttc aataaccctt gatttacctc
      541 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcggggc
      601 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg
      661 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
      721 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
      781 tggtcattga aaagccgaag ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac
      841 aggagcagcc aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa
      901 atacggcacc cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta
      961 ccagtgtggc ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag
     1021 cacaggcatt gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc
     1081 gcgtggataa cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga
     1141 aaaatgcgat gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga
     1201 atatcctgtt taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc
     1261 cgaccggagg cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca
     1321 accgctacat acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac
     1381 gctacttttt tcacccacac ttcaatatta atgctgaccg atgtcgtccc tttctggaca
     1441 cagcgtgcat agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct
     1501 tcaacccgca cagtcactac gcgaccgtgg gcaatttctt ttgccagaat agcgccgcca
     1561 atatccattt gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg
     1621 ggcatggcta aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg
     1681 gtaaaaccga cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga
     1741 acagaggaga tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct
     1801 gcggcatgtg gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg
     1861 tcaggccaaa gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga
     1921 tgtttgccag accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata
     1981 ccggttgcgg cagcgtgagt tctttactca gcatccgctg aattagcggc tttttcatca
     2041 cccattggct gactaacagg gctcccgcaa acagagcata aatgactgta accttccatt
     2101 taataaactc atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa
     2161 aagtgatcag ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa
     2221 tcgccgtggc gacgatcagc gctgcagtag ccgcatagat gtcataaatc ttgtaaaacg
     2281 cgaaaaagac aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc
     2341 aaatgcttaa ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat
     2401 ggcagaaatc aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc
     2461 ggcaaaagaa gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc
     2521 gggtgccacc agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa
     2581 aatgcccatt ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat
     2641 aattcccgga acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat
     2701 cagaataaat aacttgggca atatcggcgc actggcacca atagctcgca gcgcactgac
     2761 tctctgaccc gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt
     2821 accgattaat tctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc
     2881 cggtgacata ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc
     2941 attgagctgc gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat
     3001 aaacgcacat agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc
     3061 ggtgtcacgg tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag
     3121 gtgttaatta gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt
     3181 atggaaaagc atatctttgt aaagcggagg taattgctgg ccttcaggcg tgggcaaagc
     3241 gcggcaagtg gcgatcgagt taatatttgc gtagagaaaa tatttaaaaa ttgatttaaa
     3301 tcacattaac caggattctc aatgcaactt ctaaattaat ccagatcaat aaatggtgaa
     3361 ttatcatatg taatgtgatc tatgtaggat catttgttac tccaatgtag gtatattcgt
     3421 cacgttttta taaccataac gacggagcgg atatgaaaaa gttaacagtg gcggctttgg
     3481 cagtaacaac tcttctttct ggcagtgcct ttgcgcatga agcaggcgaa ttttttatgc
     3541 gtgcaggttc tgcaaccgta cgtccaacag aaggtgctgg tggtacgtta ggaagtctgg
     3601 gtggattcag cgtgaccaat aacactcaac tgggcttgac gtttacttat atggcgaccg
     3661 acaacattgg tgtggaatta ctggcagcga cgccgttccg ccataaaatc ggcacccggg
     3721 cgaccggcga tattgcaacc gttcaccatc tgccgccaac actgatggcg cagtggtatt
     3781 ttggtgatgc cagcagcaaa ttccgtcctt acgttggggc cggtattaac tacaccacct
     3841 tctttgataa tggatttaac gatcatggca aagaggcggg gctttccgat ctcagtctga
     3901 aagattcctg gggagctgcc gggcaggtgg gggttgatta tctgattaac cgtgactggt
     3961 tggttaacat gtcagtgtgg tacatggata tcgataccac cgccaattat aagctaggcg
     4021 gtgcacagca acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat
     4081 atcgttttta attccgcaca aaaacgaccc cgtaatatac ggggtcaata aggacatggt
     4141 ataaagggta ttatttcttc gcttctacgc catcagtttc agaacgaatt aaaaatttct
     4201 cagttgtttg cggaatatgc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa
     4261 tcgcttcgat gatcggaatt gagactgtat caccggcatt ttttgctgct gctaatagtg
     4321 aggtgtaaca ggtgatttca aattgctcga agacatatcc gccaatagag cctttaacta
     4381 tttcatcaga agggaatata ccaccgattg actgcccaag cgcagccatt ttactcatgg
     4441 aatctttaat gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa
     4501 tctggttttt ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat
     4561 ctatacggct ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt
     4621 cacgtaacca gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg
     4681 actaaaagtt aaaattcata tttcaggctt tattttcggc tttcttattt acgttattaa
     4741 gggccagatc agtcagtttg atgtcggtgg ccttttcttc ttccagggtt tctttcagaa
     4801 gcttcgctgc tttacggtaa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg
     4861 caatctcata atgctcgact ttctgtgctg cggcaatcag tgcggcatca cgcacttcgt
     4921 ttttctcggt actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac
     4981 atttcatgcg cttaattttc agatttgatt ctgattccac aacttgatca attcgttcaa
     5041 tctgtccatg agtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcatttgatg
     5101 ttgctcttgc gagttttgcc agtgcccggg ttaattgttt ttctgcgctg taggtatctg
     5161 aaagcagttg aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa
     5221 tcactgcgag ccatacggcc cgcagatgca attatcttta tcagctgata ttaataatta
     5281 acggtgaatc aggatttgcc tgatttatta ccaccgcttt gttgaccgcc ttttttaccc
     5341 gcttcagatg cgcgttgcgg atcattttta aaattaccgc cgctatgctg accgccttta
     5401 cggcctgcgt cggatgcctt ctcacggtct tcggcgaaat ttcctgaact accacgatgt
     5461 tcggccatgt tatttctccc gttgcgttgc attgtttcat taatatgagt gttgtgtgtc
     5521 gacactcatt aaaattagtc actaatgaga attagtcaaa ttaagcgcaa cgagaagata
     5581 gagggaaata tattttgagg aacattctgg atatattaac aattacctga ggaatatgtg
     5641 acttagagaa aattattgat tttactggtg ttatgttgcg ggattaattt gtttgattat
     5701 attactgttg ggcggaaaaa tgacgtaagt tgacgttcga ccggggtaag cgaaacggta
     5761 aaaagataaa tattaaatga atttaggatt tttccggctt cattaaagaa agttaaaatg
     5821 ccgccagcgg aactggcggc tgtgggatta actgcgcgtc gccgctttca tcggttgtac
     5881 aaaaactttc agtgccgcca gcattttctc tggctcatta atatgttgct cgatgatttt
     5941 aacaatggcc gaaccagaaa tcgcgcccgc agctcctgca tcaatcgctg cttttacctg
     6001 atccggggcg gaaataccaa acccctgcaa tggaggtgca gcgttgtact ctttcagctt
     6061 cgcaaccaga tgattgaggg gtaacgcggc gcggttttct gcgccggtca cgcccgctcg
     6121 tgacagcaaa tatgtgtaac cacgaccgta agaggctatc tggcgcagca ggtcgtcatc
     6181 ggcatttggc gggcagatga agataggtgc gacattatgg cgcaacgcgg cctggcggaa
     6241 gggcgcggac tcttcaactg gcacatcggc aaccagcacc gaatcgacgc cgactttctc
     6301 gcactgggca taaaactcat caatgccttt gttaaacacc agattggcat acatcaacag
     6361 gccaatggga atggtcgggt gtttctggcg aatcagtgcc agcatttcaa aacattgtgc
     6421 cggagtcaca cctgccgcaa aggcgcgcag agtggcgttt tgaatggtcg ggccatccgc
     6481 cagtgggtcg gagaagggga tacctaactc cagcgcgtca gcaccggctt caattagcgt
     6541 atcgataatt ttcaatgact gctcaatgcc cggatcaccg agcgtgacga aaggaacgaa
     6601 tgcgccttct ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt ccatcagatt
     6661 tcccctcgtg ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc gcgaccggaa
     6721 aggttaacca ccagtagctg ctctttatcc gggttttcgc gcatcatttt cagcgcatgg
     6781 gccagggcgt gggaggattc cagcgccggg atgatccctt cgtgcaggca cagcgttttg
     6841 aaggcttcaa gggcttcatc atcggtaata gacacgtaat cagcgcgtcc agtgctgtta
     6901 agatacgcgt gttgtggacc gacagacggg aaatccagtc cggcggagat ggagtaagat
     6961 tcttcaatct gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa ataaatgccc
     7021 acgcgaccat gttttagcgg tgcgccgtgc tcgccagttt cgataccgtg accaccaggc
     7081 tccacaccaa tcaggccgac gttggtttca ttgatgaaat cagcaaacat gccgatggca
     7141 ttcgaaccgc cgccaacaca ggcgataacg gcatccggca ggcgaccttc tctttccaga
     7201 atctgcgctt tggtttcttc gccaatcatc cgctgaaact cacggacaat ggtcggataa
     7261 ggatgcgggc cagccgcggt gcccagcata tagtgcgcgg tttcataact gccggaccag
     7321 tcgcgtagcg cctcattaca ggcatctttc agcgtggcgg aaccgctatg taccgggatc
     7381 acttccgcac ccattaagcg catacgaaaa acgttaggcg actggcgttc aacgtctttg
     7441 gcacccatat aaatacggca tttcaggccg agcagggcgc tggcaagggc cgacgccacg
     7501 ccatgctgac cggccccggt ttcggcgatg atttcggttt tacccatccg cttcgccagc
     7561 aacgcctgcc ccagcacctg gttggtttta tgtgcgccgc cgtgcagcaa atcttcacgc
     7621 ttgagataca gcgtggtgtt cgtcccggct gtaatgttct ggcatttggt cagtgcggtt
     7681 ggacgcccgg catagttttt cagcaggtcg ttgaactggg cttgaaattc aggatctttt
     7741 tgcgcgctga caaaagcttc ttccagctgg cgcagagcag gcatcaggat ttgtggcacg
     7801 tacatgccgc caaactcacc aaaatagggg ttaagtaatg ttgtcattgt tcctttcctt
     7861 aatatgcgcg cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg atgcccggtt
     7921 gcgactctac agcagaatta aaatcaagtc cggcgcagcc ggtttgtgcc gcttccacgc
     7981 agttatctgc gcctaagccc cccgccagca gaacgttgcc aagcgattga ccatttaata
     8041 gtgaccagtc aaaacgttgc ccgcttccac cctggccgtt gtctaaaaca tatttatcaa
     8101 cgtgctgaaa ctcgcgggcg ggcagggttt caccgacgct taatgctttc cagatggcaa
     8161 catgtgctgg cagagcttca cgcagcgtat cgatatacag ctgatcttca ttgccatgca
     8221 gttgcactgc cgccagcgat aacaccttag ctttgtccac cacatcggca atatcgtgat
     8281 tgcggaacac gccaacatac tgcaacggtg ccgcagccat cacttcctgc gcctgttcaa
     8341 cgttgacgca acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc gcgcccgcgt
     8401 cataagctgc tttagcatct tgcccacgcg tcaggccaca tactttattc tcacccagca
     8461 acacccggcg cacggcggcg tgcaaatcgt catgggccat caacgccgaa ccaatcagaa
     8521 aaccgttagc gaagtggctt aactcgcgca cctgagcgta agtattgatg ccggattcgc
     8581 tgattaccgt tacgttgtgc cccagtttcg gcgcaagctc gcgggtacgg ttgagatcaa
     8641 tcgacaaatc acgcagatcg cggttgttga tgccaacgac ctttgcccct aatgcaatgg
     8701 cgcgctccag ttcctcttca ttactgactt cggtcagcac ccccatctcc agactgtgag
     8761 cgacggctgc aagctggcga tattggtcgt catccagtac tgaaagcatt aataagcagg
     8821 catcggcctg gtaatagcgc gccagataga tctggtaagg gtcgataatg aagtctttac
     8881 ataaaatcgg ctgcggggcg atttggctga cgatgaggag gaaatcaaag ctcccctgaa
     8941 aatatttctc atcagtcagc accgaaattg ccgaagcgta atgtttataa atggcggcaa
     9001 tgcgtgccgg atcgaaatca tcacggatca cgccttttga cggcgacgct tttttacact
     9061 ccagaataaa cgccgtgcgt gcaccctgta gcgcatcata aaaatgtcgt gtgctcggct
     9121 gaacctcatt ctgaaaactg gccagcggtt gctgctgttt gcgggtttct acccaaatcg
     9181 ccttgtctgc gacgattttc gctaaaacgg tttgcatcat ttaccctcgt gccgccagcg
     9241 cggtaactct gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt tgcgcattgg
     9301 cttgcagatc ttcatggcca tgcaggcgca ttaacatggc gacgttcgca gcgacggctg
     9361 cttcatgggc ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca cggttttctt
     9421 caggtgttcc gcctgccagt tgctcctggt ggtagggagt caggccaaaa tcttcagcgg
     9481 tcaattgata gctcttaatt ttgccgtcat gcagctcggc aacgattgtc ggcgcgtgta
     9541 atgaaacttc atccatcccg ccgctgtgca ccaccgccgc gcgttgatac cccagcacgc
     9601 gcaaggtttc ggcaatcggc agcaccagtt ccggactata aacaccaatt aacgccagcg
     9661 gcggatgcgc cgggttaatc aatggcccca gcacattgaa cagggtgcgg gttttcagtt
     9721 gctggcgaac cggcatcgcg tggcggaatc cggtgtgata tttcggcgca aagaggaaac
     9781 atacgcctaa ctcgtccagc gcctggcgcg atttattggc gttcatatca agattaatac
     9841 cgaatgccgc cagcagatcc gacgagccgg atttactgga gacgctgcgg ttaccgtgtt
     9901 tcgccacttt cagcccacag gccgcggcga caaacgcact ggcggtagaa atattgatac
     9961 tgttgctgcc gtcaccgcca gtaccgacga tatcagcaaa cagataatcc gggcgcggga
    10021 acggcgctgc gttttccagt agcgcggttg ctgccccggc gatctcgttc gggtgctcac
    10081 cgcgaatttt catgctcacc agcgccgccg ccagttgttc cggcttcagc tcgccacgca
    10141 ccaccgctga aaacagctgg tggctttctt gttggctaag cgtctgcgcc tgatacagtt
    10201 tttccagaat cggttgcagc gtgttggctg gctctagttt ctgctgcgcc caggccagcg
    10261 tttgttccag caggcgagcg ccctgggtgg tgagaatgga ttccggatgg aactggaatc
    10321 cacaaacgcg atccgcatcg tgacgtactg ccatcaccat gccattaaaa tgggcgttga
    10381 tggttaaacc ggccggaatg ttactgccaa ccagcgagtg ataacgcgcc accggcagcg
    10441 ggtttgttaa tccggcaaac atcgcctgac cgtcatgttc aatgctcgac gctttaccgt
    10501 gaagaatttc gcccgcctga ccgacatagc ccccgtaagc ttcgacaatt gcctgatgtc
    10561 cgaggcaaat gccaataatg ggcagcttgc cacgcaagcg ggtgaggagt tccggcatac
    10621 aaccggcttc gctcggcaca ccggggccag gagaaagcat cagtaccgga ttgctcatcg
    10681 tcgccaggcg ttcaattaag gtctgcgccg gaatatggtt gcggtaaatc accacgttat
    10741 gaccattgct gcgcaactga tctgccaggt tgtacgtaaa agagtcgata ttatcgagca
    10801 gcagaatgtc agccatcaga aagtctcctg tgcatgatgc gcggtggcaa tagcgcgcag
    10861 tacagcgcgg gctttgttac gggtttcgtc ggcttccgac tgcggaacag aatcaaggac
    10921 tacaccagca cccgcttgca cggtggcgat accgttttcc accagcgccg agcggatcac
    10981 aatgcaggtg tcgagatcgc catgcgcggt gaaataacct accgcgccgc cgtagctgcc
    11041 gcggcgacga ccttccgcct cggcaattaa ctgcatagcg cgtactttcg gcgcaccgct
    11101 taacgtcccc atattcatac aggcgcgata agcgtgcagg gcgtcaagat cgtgacgcag
    11161 ttcgccgact acgcgagaga cgaggtgcat cacataggaa taacggtcaa ctttggtgag
    11221 atcggcgatg tagcggctgc caggggtgca aatgcgtgcc agatcattac gggcgagatc
    11281 aaccagcatc agatgttcag acagctcttt atggtcagta cgcatttcca gttcaatacg
    11341 gctgtcgaga tctctgtcca gtgaaccatc ggcgcgacga ccgcgtgggc gtgttccggc
    11401 aatcgggtag atctcaatct ggcggctggt ggcatcatac ttgagcgagc tttccggcga
    11461 cgcgccaaat agggtgaaat cattatcctg cataaaaaac atgtacgggc tgggattact
    11521 ctttttcagc acgtaatagg ccgccagcgg tgacgggcag ggcagagaga aacggcgaga
    11581 tggcaccacc tggaaaattt ctccagcgcg aatcgctttt tgcaacaaac gcactacgcc
    11641 accgaactct tcatcgctct gattacattc acaacgcata tgcggcacgg aaaccactgg
    11701 cagcggcggc gcggcttcgg tcagttgctg acgtagttcg ttcaggcgag cagtgagacg
    11761 ttgtttttct tcttcattcg gagcaaacag gctggcctga atacgggtgc tttttttctg
    11821 atggtcaatc accatcagcg tttcagcgag ataaaaacag aaatcagggc agttattttc
    11881 cgctgacagt tgcggtaaat cttcaaatcc cgccacaagg tcataagaga acaggccgcc
    11941 gaagaacatt gcttctcgtt cttccttcgg tacattcaac agattctgca ataaacggaa
    12001 agcgtcaaaa accgaaaggg agcataagcg ggcgtcttca tccagcagtg gactgacagg
    12061 ggggaagcgc agcacacggc agtttggtga ttgttcactt tccacacccg caggcagggc
    12121 gttatccagt agtgccagca gggcttcgcc attgccggaa agtgcctgaa ttgtgacggt
    12181 gtcacctaat gctgtaatgc gcagcgcact gtctatcagc agcaggcttt ttaaatcatc
    12241 tttgctgtcg atatctgcgg attccagcag cagcgttgcc ggacgattcc cacacaactg
    12301 gtgaaaaagc gcagtcgggt tgtcgcgata agcgccttcg caggttagca gttcgagagt
    12361 cggtttttgt gtttgcattg ttattctcta attttgttca aaaaaaagcc cgctcattag
    12421 gcgggctggg tatctgattg ctttacgcat ggtgaataca ctgcccgttt caggaagtgc
    12481 gccaccaacc tttcagtacg aaaattgctt tcattgtcga tacccttttt acgtgaactt
    12541 gcgtactagt taactggttc gatgattaat tgtcaacagc tcatttcaga atatttgcca
    12601 gacccgttat gatgtcggcg caaaaaacat tatccagaac gggagtgcgc cttgagcgac
    12661 acgaattatg cagtgattta cgacctgcac agccatacca cagcttccga tggctgcctg
    12721 acgccagaag cattggtgca ccgtgcagtc gagatgcgcg tcggcaccct ggcgatcacc
    12781 gaccatgaca ccacagctgc aatcgcgcca gcaagagagg aaatttcacg ttcgggactg
    12841 gcgctgaatc ttattcccgg cgtggaaatt tccacggtct gggaaaatca tgaaattcat
    12901 attgtcgggc tgaatattga tattactcat ccgctaatgt gtgagttcct cgcgcagcag
    12961 acagaacggc gcaatcagcg ggcgcagcaa attgccgaaa gacttgaaaa agcgcaaata
    13021 cctggggcgc tggaaggcgc acaacgactg gcgcagggcg gcgcagtgac gcgcggtcat
    13081 tttgcgcgtt tccttgtgga gtgcggcaaa gccagttcaa tggcagatgt ctttaaaaag
    13141 tatctggcgc gcgggaaaac cggatacgtt ccgccacagt ggtgtacaat agaacaagct
    13201 attgatgtca ttcatcattc tggcggtaag gcggtgctag ctcatcctgg gcggtacaat
    13261 cttagtgcta aatggctgaa aagattggtt gcgcattttg ccgaacacca cggcgacgcg
    13321 atggaagtcg cgcagtgcca gcaatcgccc aatgaacgca cccagctggc cacacttgcg
    13381 cgtcagcatc atttatgggc atcacaagga tctgattttc atcagccatg cccgtggatc
    13441 gaactgggtc gtaaactctg gctgcccgca ggcgttgaag gcgtctggca gctatgggaa
    13501 cagccgcaga acaccacaga gagggaatta tgagccagtt tttttatatt catcctgata
    13561 acccgcagca acgtctgatc aaccaggcgg tggagatcgt gcgtaaaggc ggggtgattg
    13621 tctatccaac tgattccggc tatgcgctcg gctgtaaaat tgaagataaa aacgccatgg
    13681 agcgtatttg tcgtattcgc cagctgccgg acggtcacaa ctttaccctg atgtgtcgcg
    13741 atctttctga actctcgacc tattcattcg tggataacgt tgcgtttcgt ttgatgaaaa
    13801 acaacacgcc gggcaactat accttcatcc tgaaggggac gaaggaagtg ccacgccgtc
    13861 tgttgcagga gaaacgcaaa accatcggta tgcgtgtgcc ttctaacccg attgcgcagg
    13921 cgttgcttga agcactgggc gaaccgatgc tttccacttc gctaatgctg ccaggcagcg
    13981 aatttaccga atccgatccc gaagaaatta aagaccgtct ggaaaaacag gtagatttga
    14041 ttattcacgg cggctatctc gggcagaaac caacaacggt tattgatctt accgatgata
    14101 cgccggtcgt ggtgcgtgaa ggcgtaggtg atgtgaagcc tttcttataa gtgcgtaact
    14161 ctgtatacta cgcggcctta aaatggcgct atacggcgcc attgattctc ctgttcgacg
    14221 cctgtgaagg cgacaccaaa aggaagctct atgagcgaaa aattacagaa agtgctggcc
    14281 cgcgctggcc acggctcccg ccgtgaaatc gaatctatta ttgaagccgg tcgtgtgagt
    14341 gttgatggca aaattgctaa actcggcgat cgcgttgaag ttacccctgg gctgaaaatt
    14401 cgtatcgatg gtcatctgat ttcggtacgt gagtccgctg aacaaatttg tcgcgttctg
    14461 gcctattaca aaccggaagg tgagttgtgt acccgtaacg atccggaagg gcgtccgacg
    14521 gtgtttgacc gtctgccaaa gctgcgtggc gcacgctgga ttgccgtagg tcgtctggac
    14581 gttaacacct gtggtctgct gctgttcacc accgatggtg aactggcaaa ccgtttaatg
    14641 catccaagcc gtgaagttga acgtgaatac gccgtgcgtg tatttggtca ggttgacgac
    14701 gcgaaactgc gtgatttgag tcgtggcgtg caactggaag atggtccggc agcttttaaa
    14761 accatcaagt tcagcggcgg cgaagggatc aaccagtggt acaacgtgac tctgaccgaa
    14821 ggccgtaacc gtgaagttcg tcgtctgtgg gaagcggttg gtgtgcaggt gagccgcctg
    14881 atccgtgttc gttacggtga tatcccactg ccaaaaggtc tgccacgcgg tggctggacg
    14941 gagctggacc tcgcccagac taactatctg cgcgaactgg tggagcttcc gccagaaacc
    15001 agctctaaag tcgctgtaga aaaagaccgt cgtcgcatga aggctaatca gattcgtcgt
    15061 gcggtgaaac gtcacagtca ggtgagcggc agtcgtcgtt ctggcgggcg taataacggt
    15121 taatcaaagt atgccggacg tcatatccgg catttttaca gattaataat caatccctat
    15181 ttgcgcctta acaccggcat caaacgcatg tctgatgggg cgcagctcac ttaccgtatc
    15241 cgccagttca agaatatccc gatgacagcc tcgacccgtg ataatcaccg tctgttgatg
    15301 tggacgttca tttaacgcct gcaccacttc ttccagtggc aaatagtcat acgccaccat
    15361 atacgtcagt tcatcaagca aaaccatatc cagtgaggaa tcagcaagca tccgctttgc
    15421 atgttgccag acttcgcggc aggcggcggt atcagactcg cggttttgtg tatcccaggt
    15481 aaagcccgtt gccatcacct gaaactcaac gccatgtggc tccagcagat tgcgttcgcc
    15541 attaggccag gtgcctttaa taaactgcac gacgcccact ttttttccgt gaccaactgc
    15601 gcgtgttgcc gtaccaaatg ccgcggtggt tttgcctttt ccattgccgg taaagacgat
    15661 gataataccg cgttcatcct gggcctgggc cacacgagca tctacttttt ctttcactcg
    15721 ctgctgacgc tgttggtagc gttcatcact cattgggaaa ttcctggttt acggcccggt
    15781 tgggcgtcaa aggtcatgcc ggttttacgg cggctgtcat cgcccatcag ccagaggtag
    15841 agcggcatga tatcagcggg tgttttaagt ttctgtggat cttcggtcgg gaaggcgctt
    15901 gcgcgcattg cggtgcgcgt accgcctggg ttaatgcagt tgacgcgcag gcgctgctga
    15961 tattcatcgg ccagtacctg catcatccct tcggtggcaa atttcgacgc tgcatatgca
    16021 ccccagttgg ctcgtccctg acgtccaacg cttgatgaag taaagaccag tgaaccggcg
    16081 tccgatttga gtaataaagg aagcagtgcc tgggtgagca taaaggtggc attaacgttg
    16141 atctgcatga cgtcctgcca gacctgcgga ttttgttcgc tcattgggca aacatcgccg
    16201 agcaatccgg cattatgcaa aacaccatcc agacgcggat aattaacgac aatgcgctgt
    16261 gccagttgtt ggcaattttc ggacgtgcag gtcagcaaat cgagaataaa ccactgtggc
    16321 tgacgcccag tttcttcgtt tatgtggctg gctacctgac gtaatttttc ttcattacgg
    16381 cccaacagaa tcactgtcgc accatagcgt gcatacgtca tcgcggcttc acgaccaatc
    16441 ccatcgctgg ctcccgtcac caggataatg cgatcattga gtaaatcttg ttttggctgg
    16501 taatgcatgg ctactcctca acgacgttgt ctgtcgcgct gccagtgtac tttatgactt
    16561 tggggcttta tgcctgaaac agaagggcat ttcaatcagc ctggggcgat aacaccgcaa
    16621 aatggatact ttgtcatact ttcgctgcaa taacatctct gcgagacggc ttaacatgcc
    16681 tgttgtaaac tgtgagccaa agcgttgttt aaccaaggtg gggactcgtg gaattgttgt
    16741 ctgaatatgg tttgtttttg gctaaaatcg ttaccgttgt gctagcgatt gcggcgattg
    16801 ccgccattat tgtcaatgtt gctcaacgta ataaacgcca gcgtggcgag ttacgggtca
    16861 acaatctcag cgaacagtat aaggagatga aagaagaact ggccgcggcg ctgatggact
    16921 cacatcagca aaaacagtgg cacaaagcgc agaagaaaaa gcacaagcaa gaagcgaaag
    16981 cagcaaaagc gaaagccaaa ctgggcgagg tggcaactga cagtaaacct cgcgtctggg
    17041 tgctggattt taaaggcagc atggacgccc atgaagtgaa ctcgctacgt gaagagataa
    17101 cggcggtact cgcagcattc aaaccgcagg atcaggttgt gctccgtctg gaaagccctg
    17161 gtggcatggt gcatggttac gggttggcgg cttcgcagct gcagcgtctg cgtgataaaa
    17221 acattccttt aactgttacg gtagacaaag tcgctgccag cggcggttac atgatggcct
    17281 gtgtggcgga caaaattgtt tccgcaccgt ttgctattgt gggttccatt ggggtggtgg
    17341 cgcaaatgcc caactttaac cgcttcctga aaagcaaaga tattgatatc gaactgcaca
    17401 ccgccgggca gtataagcgc acgctgacct tgctgggtga aaataccgaa gaagggcggg
    17461 agaaattccg cgaagagctg aacgaaacgc atcagttatt taaagatttt gtgaagcgta
    17521 tgcgtccgtc tctggatatt gaacaggtgg caacgggtga acactggtac ggacaacagg
    17581 cggtagagaa aggcctggtt gatgaaatca acaccagtga tgaagttatt cttagcctga
    17641 tggaaggccg tgaagtggtt aatgtacgct atatgcagcg taaacgactc attgaccgat
    17701 tcaccggcag cgcggcagag agcgccgatc gattgttgct acgctggtgg cagcggggtc
    17761 aaaagccatt gatgtaaaag acaaacgcga ggctaagacc tcgcgttttg ctttaatcaa
    17821 ccagatgata tttttctgaa agcacatggg ccaggtgttt gaacatatta aacaccgcgg
    17881 tgcttttggc tgttggcaat ccttgttcat ctaaaaagta gtcgccggta aataccagca
    17941 cgccattacg ctgcgtgacg ccggtggctt caatccctgc gagcgtatcc tcatgctcac
    18001 gaatgatttt gttggcctct ttcaacagcg tttcgcggtc gatgggttgt gtctctttgt
    18061 tcattattta ctccttaaac aaggacatta gtctacgcca ggcatggctt gcagacaaat
    18121 ataccacgct ggtggcaaga gcgccttact ggcaactttg gattttgcat gctaataaag
    18181 ttgcgtatcg gattttatca ggtacagtgt gacgctttcg tcaatctggc aatagatttg
    18241 cttgacattc gaccaaaaat ccgtcgtgct atagcgcctg taggccaaga cctgttaact
    18301 cagtcacctg aattttcgtg aacagagtca cgacaagggg ttgatatccg cagagagcga
    18361 gtccatatcg gtaactcgtt gccagtggaa ggtttatcaa cgtgcgacgc attcctggaa
    18421 gaatcaaatt aggtaaggtg aatatgggta aagctcttgt catcgttgag tccccggcaa
    18481 aagccaaaac gatcaacaag tatctgggtt gtgactacgt ggtgaaatcc agcgtcggtc
    18541 acatccgcga tttgccgacc agtggctcag ctgccaaaaa gagtgccgac tctacctcca
    18601 ccaagacggc taaaaagcct aaaaaggatg aacgtggcgc tctcgtcaac cgtatggggg
    18661 ttgacccgtg gcacaattgg gaggcgcact atgaagtgtt gcctggtaaa gagaaggtcg
    18721 tctctgaact gaaacaactg gctgaaaaag ccgaccacat ctatctcgca accgaccttg
    18781 accgcgaagg ggaagccatt gcatggcacc tgcgggaagt gattggtggt gatgatgcgc
    18841 gctatagccg agtggtgttt aacgaaatta ctaaaaacgc gatccgccag gcatttaaca
    18901 aaccgggtga gctgaatatt gatcgtgtta atgcccagca ggcgcgtcgc tttatggacc
    18961 gcgtggtggg gtatatggtt tcgccgctgc tgtggaaaaa gatcgctcgt ggtctgtctg
    19021 ccggtcgtgt gcagtcggtg gcggtccgcc tggtggtaga gcgtgagcgt gaaattaaag
    19081 cgttcgtacc ggaagagttc tgggaagtcg atgccagcac gactacgcca tctggtgaag
    19141 cgttggagtt gcaggtgact catcagaatg acaaaccgtt ccgtccggtc aacaaagaac
    19201 aaactcaggc tgcggtaagt ctgctggaaa aagcgcgcta cagcgtgctg gaacgtgaag
    19261 acaaaccgac aaccagtaaa cctggcgctc cttttattac ctctacgctg caacaagctg
    19321 ccagcacccg tcttggtttt ggcgtgaaaa aaaccatgat gatggcgcag cgtttgtatg
    19381 aagcaggcta tatcacttac atgcgtaccg actccactaa cctgagtcag gacgcggtaa
    19441 atatggttcg cggttatatc agcgataatt ttggtaagaa atatctgccg gaaagtccga
    19501 atcagtacgc cagcaaagaa aactcacagg aagcgcacga agcgattcgt ccttctgacg
    19561 tcaatgtgat ggcggaatcg ctgaaggata tggaagcaga tgcgcagaaa ctgtaccagt
    19621 taatctggcg tcagttcgtt gcctgccaga tgaccccagc gaaatatgac tccacgacgc
    19681 tgaccgttgg tgcgggcgat ttccgcctga aagcacgcgg tcgtattttg cgctttgatg
    19741 gctggacaaa agtgatgcct gcactgcgta aaggcgatga agatcgtatc ttaccggcag
    19801 tcgataaagg cgatgctctg acgctcgttg aactaacacc agcccagcac tttaccaagc
    19861 cgccagcccg tttcagtgaa gcatcgctgg ttaaagaact ggaaaaacgt ggtatcggtc
    19921 gtccgtctac ctatgcgtcg atcatttcga ccattcagga tcgtggctat gtgcgagtag
    19981 aaaatcgtcg tttctatgcg gaaaaaatgg gcgaaatcgt caccgatcgc cttgaagaaa
    20041 atttccgcga gttaatgaac tacgacttta ccgcgcagat ggaaaacagc ctcgaccagg
    20101 tggcaaatca cgaagcagag tggaaagctg tactggatca cttcttctcg gatttcaccc
    20161 agcagttaga taaagctgaa aaagatccgg aagagggtgg tatgcgcccg aaccagatgg
    20221 ttctgaccag cattgactgc ccgacttgtg gtcgcaaaat ggggattcgc acagcgagta
    20281 ccggggtatt ccttggctgt tctggctatg cgctgccgcc gaaagagcgt tgcaaaacca
    20341 ccattaacct ggtgccggaa aacgaagtgc tgaacgtgct ggaaggcgaa gatgctgaaa
    20401 ccaacgcgct gcgcgcaaaa cgtcgttgcc cgaaatgcgg cacggcgatg gacagctatc
    20461 tcatcgatcc gaaacgtaag ttgcatgtct gtggtaataa cccaacctgc gacggttacg
    20521 agatcgaaga gggcgaattc cgcattaaag gttatgacgg cccgatcgtt gagtgtgaaa
    20581 aatgtggctc tgaaatgcac ctgaaaatgg ggcgtttcgg taaatacatg gcctgcacca
    20641 acgaagagtg taaaaacaca cgtaagattt tacgtaacgg tgaagtggca ccaccgaaag
    20701 aagatccggt gccattacct gagctgccgt gcgaaaaatc agatgcttat ttcgtgctgc
    20761 gtgacggtgc tgccggtgtg ttcctggctg ccaacacttt cccgaaatcg cgtgaaacgc
    20821 gtgcgccact ggtggaagag ctttatcgct tccgcgaccg tctgccggaa aaactgcgtt
    20881 atctggccga tgcgccacag caggatccgg aaggtaataa gaccatggtt cgctttagcc
    20941 gtaaaaccaa acagcaatat gtctcttcgg aaaaagacgg aaaggcgact ggctggtcag
    21001 cattttatgt tgatggcaaa tgggttgaag gaaaaaataa cctttaattc tgtcaggttt
    21061 ttataaacaa agggtcgcga aagcggccct tttttattgc atattatttt ttcttcacac
    21121 ctatacacta aggctataaa tgatatagtg gttatagtta gcaccttttt tattattaaa
    21181 tcgtattagt cacccgccag gtgtgacgaa aaaacgatgt tctgatggcg tctaagtgga
    21241 tggtttaaca tgaaattaca acaacttcgc tatattgttg aggtggtcaa tcataacctg
    21301 aatgtctcat caacagcgga aggactttac acatcacaac ccgggatcag taaacaagtc
    21361 agaatgctgg aagacgagct aggcattcaa attttttccc gaagcggcaa gcacctgacg
    21421 caggtaacgc cagcaggaca agaaataatt cgtatcgctc gcgaagtcct gtcgaaagtc
    21481 gatgccataa aatcggttgc cggtgagcac acctggccgg ataaaggctc gctgtatatc
    21541 gccaccacgc atacccaggc acgctacgcg ttaccaaacg ttatcaaagg ctttattgag
    21601 cgttatcccc gcgtttcttt gcatatgcac cagggcccgc cgacacaaat tgctgatgcc
    21661 gtctctaaag gcaatgccga tttcgctatc gccacagaag cgctgcatct gtatgaagat
    21721 ttagtgatgt taccgtgcta ccactggaat cgggctattg tagtcactcc ggatcacccg
    21781 ctggcaggca aaaaagccat taccattgaa gaactggcgc aatatccgtt ggtgacatat
    21841 accttcggct ttaccggacg ttcagaactg gatactgcct ttaaccgcgc agggttaacg
    21901 ccgcgtatcg ttttcacggc aacggatgct gacgtcatta aaacttacgt ccggttaggg
    21961 ctgggggtag gggtcattgc cagcatggcg gtggatccgg tcgccgatcc cgaccttgtg
    22021 cgcgttgatg ctcacgatat cttcagccac agtacaacca aaattggttt tcgccgtagt
    22081 acttttttgc gcagttatat gtatgatttc attcagcgtt ttgcaccgca tttaacgcgt
    22141 gatgtcgttg atgcggctgt cgcattgcgc tccaatgaag aaattgaggt catgtttaaa
    22201 gatataaaac tgccggaaaa ataatttctt gcgttatttt cggcaccttt tatgtagcga
    22261 aggtgccgga atatattctc ttttgttact tatttctata ccgtgcacaa tatttttatc
    22321 cctcatattt atagggtaaa ttacctgtaa agtagtgtct aattacccgc ctcgcaaatt
    22381 ccagttttca aatatctatt tcaggtcaaa agattgaatt attcactgga gacgattcgt
    22441 aaattcgctg gatttttgac taaagtttct ttaggatttg tctcatcgta tgattaatta
    22501 caccaatcat tcaacgtctt gctgataagt gatggcgatt atatgaggtt agcaatgcct
    22561 tctggaaatc aggaaccccg ccgtgatcct gaattaaaac gtaaagcctg gctggcggtt
    22621 tttcttggtt ctgcactttt ctgggtggtt gtcgcactgc tgatttggaa agtgtgggga
    22681 taactatggt cggtcaggag caactggagt cgtcaccatt atgccagcat agtgacaatg
    22741 aaccagaaac gaaacgggaa tgttccgtcg ttattccaga cgactggcaa ctaacatcgc
    22801 agcagcaagc ctttatagaa ctgtttgctg aagatgatca gccgaaacaa taattatcat
    22861 cattcttatt acccattttt aatgaattaa agggctttta atacaccgca gcaataacag
    22921 cttgagttat ctcaacacaa aataataacc gttaagggtg tagcctatga tcaacacaaa
    22981 tatgaaatat tggtcctgga tgggcgcgtt ttctctgtcg atgctcttct gggccgaact
    23041 cctctggatc attactcact gatccttgac cccgctgcgg cggggttgtc atttgctttg
    23101 ccacaaggtt tctcctcttt tatcaatttg ggttgttatc aaatcgttac gcgatgtttg
    23161 tgttatcttt aatattcacc ctgaagagaa tcagggcttc gcaaccctgt cattaaggag
    23221 gagctatgtc gtcaacccta cgagaagcca gtaaggacac gttgcaggcc aaagataaaa
    23281 cttaccacta ctacagcctg ccgcttgctg ctaaatcact gggcgatatc acccgtctac
    23341 ccaagtcact caaagttttg ctcgaaaacc tgctgcgctg gcaggatggt aactcagtta
    23401 ccgaagagga tatccacgca ctggcgggat ggctgaaaaa tgcccatgct gaccgtgaaa
    23461 ttgcctaccg cccggcaagg gtgctgatgc aggactttac cggcgtacct gccgttgttg
    23521 atctggcggc aatgcgcgaa gcggttaaac gcctcggtgg cgatactgca aaggttaacc
    23581 cgctctcacc ggtcgacctg gtcattgacc actcggtgac cgtcgatcgt tttggtgatg
    23641 atgaggcatt tgaagaaaac gtacgcctgg aaatggagcg caaccacgaa cgttatgtgt
    23701 tcctgaaatg gggaaagcaa gcgttcagtc ggtttagcgt cgtgccgcca ggcacaggca
    23761 tttgccatca ggttaacctc gaatatctcg gcaaagcagt gtggagcgaa ttgcaggacg
    23821 gtgaatggat tgcttatccg gatacactcg ttggtactga ctcgcacacc actatgatca
    23881 acggccttgg cgtgctgggg tggggcgtgg gtgggatcga agcagaagcc gccatgttag
    23941 gccagccggt ttccatgctt atcccggacg tagtgggctt taaacttacc ggaaaattac
    24001 gtgaaggtat taccgccaca gacctggttc tcactgttac ccaaatgctg cgcaaacatg
    24061 gcgtggtggg gaaattcgtc gaattttatg gtgatggtct ggattcacta ccgttggcgg
    24121 atcgcgccac cattgccaat atgtcgccag aatatggtgc cacctgtggc ttcttcccaa
    24181 tcgatgcagt aaccctcgat tacatgcgtt taagcgggcg cagcgaagat caggtcgagt
    24241 tggtcgaaaa atatgccaaa gcgcagggca tgtggcgtaa cccgggcgat gaaccgattt
    24301 ttaccagtat gttagaactg gatatgaatg acgttgaagc gagcctggca gggccaaaac
    24361 gcccacagga tcgcgttgca ctgcccgatg taccaaaagc atttgccgcc agtaacgaac
    24421 tgaaagtgaa tgccacgcat aaagatcgcc tgccagtcga ttatgttatg aacggacatc
    24481 agtatcagtt acctgatggc gctgtggtca ttgctgcgat aacctcgtgc accaacacct
    24541 ctaacccaag tgtgctgatg gccgcaggct tgctggcgaa aaaagccgta actcttggcc
    24601 tcaagcggca accgtgggtc aaagcgtcgt tggcaccggg ttcgaaagtc gtttctgatt
    24661 atctggcaaa agcgaaactg acaccgtatc tcgacgaact ggggtttaac cttgtgggat
    24721 acggttgtac cacctgtatt ggtaactctg ggccgctgcc cgatcctatc gaaacggcaa
    24781 tcaaaaaagg cgatttaacc gtcggtgcgg tgctgtccgg caaccgtaac tttgaaggcc
    24841 gtatccatcc gctggttaaa actaactggc tggcctcgcc accgctggtg gttgcctatg
    24901 cgctggcggg aaatatgaat atcaacctgg cttctgagcc tatcggccat gatcgcaaag
    24961 gcgagccggt ttatctgaaa gatatctggc catcggcaca agaaattgcc cgtgcggtag
    25021 aacaagtctc cacagaaatg ttccgcaaag agtacgcaga agtttttgaa ggcacagcag
    25081 agtggaagga aattaacgtc acacgatccg atacctacgg ttggcaggag gactcaacct
    25141 atattcgctt atcgcctttc tttgatgaaa tgcaggcaac accagcacca gtggaagata
    25201 ttcacggtgc gcggatcctc gcaatgctgg gggattcagt caccactgac catatctctc
    25261 cggcgggcag tattaagccc gacagcccag cgggtcgata tctacaaggt cggggtgttg
    25321 agcgaaaaga ctttaactcc tacggttcgc ggcgtggtaa ccatgaagtg atgatgcgcg
    25381 gcaccttcgc caatattcgc atccgtaatg aaatggtgcc tggcgttgaa ggggggatga
    25441 cgcggcattt acctgacagc gacgtagtct ctatttatga tgctgcgatg cgctataagc
    25501 aggagcaaac gccgctggcg gtgattgccg ggaaagagta tggatcaggc tcaagccgtg
    25561 actgggcggc aaaaggacct cgtctgcttg gaattcgtgt ggtgattgcc gaatcgtttg
    25621 aacgaattca ccgttcgaat ttaattggca tgggcatcct gccgctggaa tttccgcaag
    25681 gcgtgacgcg taaaacgtta gggctaaccg gggaagagaa gattgatatt ggcgatctgc
    25741 aaaatctaca acctggcgcg acggttccgg tgacgcttac gcgcgcggat ggtagccagg
    25801 aagtcgtacc ctgccgttgt cgtatcgaca ccgcgacgga gttgacctac taccagaacg
    25861 acggcatttt gcattatgtc attcgtaata tgttgaagta acaagtattt gcttgccggt
    25921 tattttgctt ccggcaagca aatgaattac acgatgcaag agggttattt gttcagcaaa
    25981 tggcccattt tctcggcttt ggtatcgaga taatgttcgt tattggggtt acgacctaca
    26041 atcaatggta cgcgttcaac aatattaatc cctgcttcgg tcagaatttc gacttttttc
    26101 gggttattgg ttaacaagcg gacttcattg acgccaagga gtttgaacat atcagcgcaa
    26161 agagtgaagt cgcgctcatc agcggcgaag cctaactggt ggttagcctc tacggtatcg
    26221 taaccttgat cctgcagtgc gtaagcgcgg attttattca gcagaccaat gttacgacct
    26281 tcctgacggt gatacagcaa aataccacgg ccttcctcgg caatttgcgt caatgccgct
    26341 tcgagctgga agccacaatc gcagcgcaag ctgaacaggg cgtcaccggt cagacattcg
    26401 gaatggacgc gcgcaagtac cggggtatgc ccggaaatat cgccatagac tagcgcaaca
    26461 tgatcgtgtc cggttgccag ttcttcaaat cccaccatca ggaaatcgcc ccatggggtt
    26521 ggcagtttgg cttctgccac acgtttaagc tgcatgaaat tctccagata atgctggttc
    26581 tgtattggct tattttgcca taacgagaag gggttcacct aatcacgacg cgtcgatcgt
    26641 tcacggaatg gcacaaatct gtcaattttt cttggaactg gcattttcag ttatgattgt
    26701 gggacttatc aaaaaggaga ggccatgcgt tcgattgccc gacgtaccgc agtgggagct
    26761 gcactattgc ttgtcatgcc agtagccgta tggatttctg gctggcgttg gcaacctgga
    26821 gaacaaagtt ggctactaaa agcggctttt tgggttactg aaactgtcac ccagccctgg
    26881 ggcgtcatta cacatttgat tttgttcggc tggtttctct ggtgtctgcg ttttcgcatt
    26941 aaggctgcct ttgtattatt tgccattctg gcggcggcaa tccttgttgg acaaggcgtt
    27001 aaatcctgga tcaaagacaa agtacaggaa ccacgacctt ttgttatctg gttggaaaaa
    27061 acacatcata ttccggttga tgagttctac actttaaagc gagcagaacg cggaaatcta
    27121 gtaaaagaac agttggctga agagaaaaat attccacaat atttgcgttc acactggcag
    27181 aaagagacgg ggtttgcctt tccttccggt cacacgatgt ttgctgccag ttgggcactg
    27241 ctggccgttg gtttgctgtg gccgcgtcgg cgaacgttaa ccattgctat cttgttggtc
    27301 tgggcaacgg gagtcatggg aagccgcctg ctgctcggga tgcattggcc acgcgatctg
    27361 gtggtagcta cgttgatttc gtgggcgctg gtggcggtgg caacgtggct tgcgcaacga
    27421 atttgtgggc cattaacacc acctgcggaa gaaaatcgcg aaattgcaca acgagaacaa
    27481 gaaagttaac gctggttgat tttccgaatt taggccttaa atcatcaaca atgcgcgtgg
    27541 atgccatttc gcagacggcg cgaaaatggt actttaaagg gctattgcgg taagttgacc
    27601 ataatttatt cgctctaacc acataacggg aagtaatgtg aaatatttac tcattttctt
    27661 actggtgtta gcgatcttcg tgatttcggt cacgttgggt gcgcagaacg atcaacaggt
    27721 gacgtttaat tatctgttag cgcaagggga gtaccgtatt tccacattgc tggcggtatt
    27781 gtttgctgcg gggtttgcta tcggttggtt gatttgtggc ctgttctggc tgcgagttcg
    27841 tgtttccctg gcgcgcgctg aacgtaaaat aaagcgactg gaaaaccagc tttcacccgc
    27901 gactgacgtg gctgtagtgc cgcactcgtc agcggcgaag gaataacttt ctatgctgga
    27961 gttgttgttt ctgcttttgc ctgtagccgc tgcctatggc tggtatatgg gccgcagaag
    28021 tgcgcaacaa aacaagcaag atgaagccaa ccgcttgtcg cgtgattacg tagcgggggt
    28081 taacttcctg cttagtaatc aacaggataa agcggtagac ctgtttctcg atatgcttaa
    28141 agaggataca ggcaccgttg aagcccacct tacgctcgga aacctgttcc gttcgcgtgg
    28201 cgaagttgat cgcgctattc gcatccatca gaccctaatg gaaagcgcct cgctgaccta
    28261 tgaacagcgt ctgttggcga ttcaacaact ggggcgtgat tacatggccg ccgggttata
    28321 cgaccgcgcg gaagacatgt tcaatcagct gaccgatgaa actgacttcc gcattggcgc
    28381 gctgcaacag ttgctacaaa tctaccaggc taccagtgag tggcagaaag caattgatgt
    28441 tgccgaacgc ctggtgaagc tgggtaaaga taaacagcgc gtcgaaattg cccatttcta
    28501 ctgtgagtta gccctgcagc atatggccag cgacgatctc gatcgtgcca tgaccttgct
    28561 aaaaaaaggg gcggcggcag ataaaaacag cgcccgcgta tccatcatga tgggacgcgt
    28621 gtttatggcg aaaggagaat acgccaaagc cgtcgaaagt ttgcaacgcg tcatatccca
    28681 ggacagagaa ctggtcagcg aaacgctgga aatgttgcaa acctgctatc agcagttggg
    28741 taaaactgcc gaatgggcag aattcctgca acgcgcggtg gaagagaaca ccggtgccga
    28801 tgctgaattg atgcttgctg atatcatcga agcgcgcgac ggtagtgagg ctgcacaggt
    28861 ctatattaca cgccagcttc agcgtcatcc gaccatgcgt gtattccata agctaatgga
    28921 ttaccactta aatgaagcgg aagaagggcg cgccaaagag agtctgatgg tgctgcgtga
    28981 catggttggc gagaaggtgc gtagtaagcc tcgttatagc tgccagaaat gtggttttac
    29041 cgcatacacc ctctactggc attgtccgtc ttgtcgtgcc tggtcaacta ttaaaccgat
    29101 tcgcggtctt gatggcctgt aatttttaaa aaaatccgac tttagttaca acatactagt
    29161 tattgatgtc ccattgtgct ccggcaacga cggagcagaa aaagcctgct aggggaaaaa
    29221 tcgcaactgt taatttttta ttttcacggg tagaatgctc gccgtttacc tgtttcgcgc
    29281 cactcccggt gcccatcatc aagaaggtct ggtcatgacg ttaactgctt catcttcttc
    29341 ccgcgccgtt acgaattctc ctgtggttgt tgcccttgat tatcataatt gtgatgacgc
    29401 gctgtccttt gtcgacaaga tcgacccacg cgattgtcgt ctgaaggtcg gtaaagagat
    29461 gtttacattg tttgggccac agtttgtgcg cgaacttcaa cagcgtggtt ttgatatctt
    29521 tcttgacctg aaattccacg atatccccaa cactgcagcg cacgctgtcg ctgctgcagc
    29581 tgacttaggc gtgtggatgg tgaatgttca tgcctccggt ggggcgcgta tgatgaccgc
    29641 agcgcgtgag gcactggttc cgtttggcaa agatgcaccg cttttgattg ctgtgacagt
    29701 gttgaccagc atggaagcca gcgacctggc cgatcttggc gtgacactgt cacctgcaga
    29761 ttatgcagaa cgtctggcag cactgacgca aaaatgtgac cttgatggtg tggtgtgttc
    29821 tgctcaggaa gctgtgcgct ttaaacaggt attcggtcag gagttcaaac tggttacacc
    29881 gggcattcgt ccgcagggga gtgaagctgg tgaccagcgc cgcattatga cgccagaaca
    29941 ggcgttggcg gctggtgttg attatatggt gattggtcgc ccggtaacgc aatcggtaga
    30001 tccagcgcag acactgaaag cgatcaacgc ctctttacag cggagtgcat gatgagtgat
    30061 tccaacagcc gtctggtcta ctcaacggaa accgggcgta ttgatgagcc caaagcggcc
    30121 ccagtacgcc ctaaaggcga tggagcggtg cgtattcagc gtcagaccag tggacgtaaa
    30181 ggtaagggcg tttgcctgat taccggtatc gatctcgatg atgccgaact gacaaaactt
    30241 gcagcggaac tgaagaaaaa atgcggctgc ggcggagcag ttaaagatgg agttattgaa
    30301 atccagggcg ataagcgtga tttattaaaa tcactacttg aagcgaaagg gatgaaggta
    30361 aaactcgcag gcggttaaca taaaaagcca cggatatagc cgtggctttc gaatatttta
    30421 ctgtgtgtat tattcagtta tttcgtactg aacagaatca agcgtattat aattgcgagt
    30481 cgataatcga catttattta cgattattta ccgacctggt gaccaataac accaccgaca
    30541 gctgcaccac ctaatgtacc caacgtactg ccatcggtca gtactgcacc gcctaatgcc
    30601 cctgcacccg cgccgattgc ggtgttgcgg tcccgtttag accagttaga acaggcactc
    30661 agagacattg ccaaagtaat tgccagaaca gccgcggtca tttttttgct cgttacaaac
    30721 ataatactct ctcctgaatt tatgattcac ggaagtaagc tctctataac tataatagct
    30781 aagaataagc ctggtgaaat tattccgtga aatctgctcg cgcgcagggt tatatcacgc
    30841 aggtgataat tacttcctgt tatatcgctg at
//
LOCUS       DAZSNM010000020        30774 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZSNM010000020 DAZSNM010000000
VERSION     DAZSNM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30774
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.020"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(98..952)
                     /gene="ampE"
                     /locus_tag="ACHW42_000830"
     CDS             complement(98..952)
                     /gene="ampE"
                     /locus_tag="ACHW42_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR8942768.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(949..1500)
                     /gene="ampD"
                     /locus_tag="ACHW42_000831"
     CDS             complement(949..1500)
                     /gene="ampD"
                     /locus_tag="ACHW42_000831"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR8942769.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            1588..2481
                     /gene="nadC"
                     /locus_tag="ACHW42_000832"
     CDS             1588..2481
                     /gene="nadC"
                     /locus_tag="ACHW42_000832"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR8942770.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            2684..3124
                     /gene="ppdD"
                     /locus_tag="ACHW42_000833"
     CDS             2684..3124
                     /gene="ppdD"
                     /locus_tag="ACHW42_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR8942771.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            3134..4281
                     /locus_tag="ACHW42_000834"
                     /pseudo
     CDS             3134..4281
                     /locus_tag="ACHW42_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            4271..5473
                     /gene="hofC"
                     /locus_tag="ACHW42_000835"
     CDS             4271..5473
                     /gene="hofC"
                     /locus_tag="ACHW42_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR8942772.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(5508..6551)
                     /gene="guaC"
                     /locus_tag="ACHW42_000836"
     CDS             complement(5508..6551)
                     /gene="guaC"
                     /locus_tag="ACHW42_000836"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR8942773.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(6713..6751)
                     /locus_tag="ACHW42_000837"
                     /pseudo
     CDS             complement(6713..6751)
                     /locus_tag="ACHW42_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6776..7396
                     /gene="coaE"
                     /locus_tag="ACHW42_000838"
     CDS             6776..7396
                     /gene="coaE"
                     /locus_tag="ACHW42_000838"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR8942774.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            7396..8139
                     /gene="zapD"
                     /locus_tag="ACHW42_000839"
     CDS             7396..8139
                     /gene="zapD"
                     /locus_tag="ACHW42_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR8942775.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            8149..8346
                     /gene="yacG"
                     /locus_tag="ACHW42_000840"
     CDS             8149..8346
                     /gene="yacG"
                     /locus_tag="ACHW42_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR8942776.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(8446..8835)
                     /gene="mutT"
                     /locus_tag="ACHW42_000841"
     CDS             complement(8446..8835)
                     /gene="mutT"
                     /locus_tag="ACHW42_000841"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR8942777.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(8895..11600)
                     /gene="secA"
                     /locus_tag="ACHW42_000842"
     CDS             complement(8895..11600)
                     /gene="secA"
                     /locus_tag="ACHW42_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR8942778.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(11662..12174)
                     /gene="secM"
                     /locus_tag="ACHW42_000843"
     CDS             complement(11662..12174)
                     /gene="secM"
                     /locus_tag="ACHW42_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR8942779.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(12405..13322)
                     /gene="lpxC"
                     /locus_tag="ACHW42_000844"
     CDS             complement(12405..13322)
                     /gene="lpxC"
                     /locus_tag="ACHW42_000844"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR8942780.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(13423..14574)
                     /gene="ftsZ"
                     /locus_tag="ACHW42_000845"
     CDS             complement(13423..14574)
                     /gene="ftsZ"
                     /locus_tag="ACHW42_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR8942781.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(14635..15897)
                     /gene="ftsA"
                     /locus_tag="ACHW42_000846"
     CDS             complement(14635..15897)
                     /gene="ftsA"
                     /locus_tag="ACHW42_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR8942782.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(15894..16724)
                     /gene="ftsQ"
                     /locus_tag="ACHW42_000847"
     CDS             complement(15894..16724)
                     /gene="ftsQ"
                     /locus_tag="ACHW42_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR8942783.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(16726..17646)
                     /gene="ddlB"
                     /locus_tag="ACHW42_000848"
     CDS             complement(16726..17646)
                     /gene="ddlB"
                     /locus_tag="ACHW42_000848"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR8942784.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(17639..19114)
                     /gene="murC"
                     /locus_tag="ACHW42_000849"
     CDS             complement(17639..19114)
                     /gene="murC"
                     /locus_tag="ACHW42_000849"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR8942785.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(19168..20235)
                     /gene="murG"
                     /locus_tag="ACHW42_000850"
     CDS             complement(19168..20235)
                     /gene="murG"
                     /locus_tag="ACHW42_000850"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR8942786.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(20232..21476)
                     /gene="ftsW"
                     /locus_tag="ACHW42_000851"
     CDS             complement(20232..21476)
                     /gene="ftsW"
                     /locus_tag="ACHW42_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR8942787.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(21476..22792)
                     /gene="murD"
                     /locus_tag="ACHW42_000852"
     CDS             complement(21476..22792)
                     /gene="murD"
                     /locus_tag="ACHW42_000852"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR8942788.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(22795..23877)
                     /gene="mraY"
                     /locus_tag="ACHW42_000853"
     CDS             complement(22795..23877)
                     /gene="mraY"
                     /locus_tag="ACHW42_000853"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR8942789.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(23871..25229)
                     /gene="murF"
                     /locus_tag="ACHW42_000854"
     CDS             complement(23871..25229)
                     /gene="murF"
                     /locus_tag="ACHW42_000854"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR8942790.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(25226..26713)
                     /gene="murE"
                     /locus_tag="ACHW42_000855"
     CDS             complement(25226..26713)
                     /gene="murE"
                     /locus_tag="ACHW42_000855"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR8942791.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(26700..28466)
                     /gene="ftsI"
                     /locus_tag="ACHW42_000856"
     CDS             complement(26700..28466)
                     /gene="ftsI"
                     /locus_tag="ACHW42_000856"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR8942792.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSVLVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(28482..28847)
                     /gene="ftsL"
                     /locus_tag="ACHW42_000857"
     CDS             complement(28482..28847)
                     /gene="ftsL"
                     /locus_tag="ACHW42_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR8942793.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(28844..29785)
                     /gene="rsmH"
                     /locus_tag="ACHW42_000858"
     CDS             complement(28844..29785)
                     /gene="rsmH"
                     /locus_tag="ACHW42_000858"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR8942794.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(29787..30245)
                     /gene="mraZ"
                     /locus_tag="ACHW42_000859"
     CDS             complement(29787..30245)
                     /gene="mraZ"
                     /locus_tag="ACHW42_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR8942795.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         7368 a         8701 c         7534 g         7171 t
ORIGIN      
        1 aggtatcggc tatctgttta aagagaaaac cgccaaagcc gtaaaagcgc attaatctct
       61 ctacgccctc acccgtacag ggtgagggca ataatctcta caccaacgcc ccgtaaatcg
      121 tcagtagcgc aatcaccacc acgaccacga acgaggtttt cttcgccatt gaaaccgctg
      181 ccttcggcgt ctccacctta tcgacatgcg gttcacgcgc cagagagaac tgcgccagac
      241 gcgttaatac ctgatattgc gaagtatgga aatcacccag cgaagcaaac caggccggta
      301 acgctttctc accatgaccg atcaaggcat ataccacacc cgcaagacga accggcaccc
      361 aatccagtac atgaagcact gcatcaatgc cggactgtaa acgatgatgc ggcgtctggt
      421 atcgcgccag ccagtattgc catgcacgca agaacgcata ccccatcagc gtaacgggtc
      481 cccaggttcc ccccacaatc agccagaaca gcggtgcaag ataaaaacga aagttaatcc
      541 acagcaatgc attttgcagc tcacgcaaat actcacgttc gtcgcagcct gccgggacgc
      601 cgtgaatcat ggtgagttcg ccagccatcg tggcacgggc atggctatca ttacgtgaag
      661 cagctgtcag ataagcatga taatgaagac gaactttacc tgcgccaata cacagcaaac
      721 caatcagcag ccacaccagc agcgtgggaa cgttgaacaa tactccctgc aatgcgcgta
      781 acagtaaaaa agtcacaccc atcgcaataa tggtcatgcc taacgtgcgc ccgagagaaa
      841 aatgtttcac ccgccgaaag aacgcttcaa gacgatgatc aagctgccag tgctcgccca
      901 acttaaacag gcgctcgaaa attaacacca gtaaggttgt aaatagcgtc atgttgtctc
      961 cttgctgacc agcgcacgaa accgtgccca atcaaatgca ggaccgggat cggttttccg
     1021 atccggcgca atatcacaat ggcccgtcat gttattagcg atatcgggat agcgatctat
     1081 cagtgcccgc gtaaccgccg caagctgttg atactgcgca tcggtatacg ccagagtatc
     1141 ggtgccttca agctcaatcc caatagaaaa atcattgcag cgttcacgcc cctgatactg
     1201 agagactccc gcatgccagg cacgtttatc gaaaggaaca tactggacta tttcaccatc
     1261 acggcgaatc aaacagtgag cggagacgcg caaatgggcg atctcagcaa agaaaggatg
     1321 tgcctgcgga tcaatagttc cagtgaataa tgcgtcgatc cacggaccgc caaactcgcc
     1381 tggcggcagg ctaatattgt gcaccaccag cagggtgggt gtttcgtcat ccaggcggca
     1441 atcgtaatgt ggtgagggaa cgcggcgcgc gccaaccagc cacccctgtt ctaacaacat
     1501 gcaggatctc cttatatgtg gtgctaatac ccggttcaga gtagcatgtt tctaccttat
     1561 gattcgttag ctatctggag ttttaacatg ccgcctcgcc gctacaaccc tgacacccga
     1621 cgtgacgagc tgctggaacg cattaatctc gatatccccg gcgcggtggc ccaggcgctg
     1681 cgggaagatt taggcggaac agtcgatgcc aacaatgaca ttacggcaaa acttttaccg
     1741 gaaaattctc gctctcatgc cacggtgatc acccgcgaga atggcgtatt ttgtggcaaa
     1801 cgctgggttg aagaggtgtt tattcaactg gcaggcgacg atgtcaccat aatctggcat
     1861 gtggatgacg gcgatgtcat caacgccaat caacccttgt tcgaacttga aggcccatcc
     1921 cgcgtgctgt taacgggcga acgcactgcg cttaattttg tgcaaaccct ttcaggagtt
     1981 gccagtaagg tacgccacta tgtcgaatta ctggaaggca ccaacacgca gttgttggat
     2041 acgcgcaaaa ccttacctgg cctgcgttca gctctgaaat acgcggtact ttgcggcggc
     2101 ggagcgaatc accgtctggg gctttctgat gccttcctga tcaaagaaaa ccatattatt
     2161 gtctccggct cagtgcgcca ggcggtcgaa aaagcgtcct ggctgcatcc ggatgcgcca
     2221 gtagaagtcg aagtagagaa tctggaagaa cttgatgagg ccctgaaagc cggagccgat
     2281 atcatcatgc tggataactt cgaaacagaa cagatgcgcg aagccgtcaa acgcaccaac
     2341 ggcaaggcgc tactggaagt gtctggcaac gtcactgaca aaacactgcg cgaatttgcc
     2401 gaaacgggcg tggactttat ctccgtcggt gcgctaacta aacacgtaca agcactcgac
     2461 ctttcaatgc gttttcgcta attgctgaca gatttatccg ctccatcgcg ggcggataat
     2521 ctccccttcc ccaactttct tcgtaacgcc tcgcaaattt cttgtctttc gctgcaacga
     2581 aataaaaaac ctcggaatct gctttcccgt ttcgtttttt gcccctcgcc tgtgctgatg
     2641 gcgtttgcca aagtagcacc aaccaaatca aggagcgaaa cagatggaca agcaacgcgg
     2701 ttttacactt atcgaactga tggtggttat tggcatcatt gccattttaa gctccattgg
     2761 tattcccgct tatcaaaact acctgcgcaa agccgcactc accgacatgc tacaaacctt
     2821 tgtgccttac cgtaccgccg tagagttgtg cgcgctggaa catggtggat tagatacctg
     2881 cgacggtggc agcaatggca ttccctcgcc taccaccacc cgctatgttt cagccatgag
     2941 tgtggcaaag ggcgtggtgt cgctgaccgg gcaagaaagt ctcaatgggc taagcgtcgt
     3001 catgacgcca ggttgggata acgcaaacgg cgtcaccggc tgggcgcgca actgcaatat
     3061 tcaaagtgac agcgcattgc agcaagcctg cgaagatgtc ttccgctttg atgacgccaa
     3121 ctaaggagcg gcaatgaata ttccacagct cacggccctg tgcctgcgtt atcagggagt
     3181 cttgctggat gccagcgaag aagtggttca tgttgcggtg gtcgatgccc cctcacatga
     3241 gttgctggac gcattgcatt tcgctaccac caaacgtatt gagatcacct gctggacgcg
     3301 ccaacaaatg gaaggtcacg ccagtcgcac acaacagaca ttacccgtag ctgttcagga
     3361 gaagcatcag cccaaagcag agttgctgac tcgaacgtta caatctgcgc tggaacaacg
     3421 cgcgtctgat attcatatcg aaccagcgga caatgcctac cgcatccgct tgcgtatcga
     3481 cggcgtattg catcctttac cggatgtttc accggatgcc ggagtcgcat taacagccag
     3541 attaaaagtg ctgggaaacc tggatattgc ggaacatcgc ctgccgcagg acgggcaatt
     3601 cactgtcgaa ctggcaggaa acaccgtctc atttcgtatt gcgaccttac catgtcgggg
     3661 tggtgaaaag gtggtattaa ggttgttaca gcaggtgagc caggcactgg atgttaacac
     3721 gctgggaatg cagccgttac aactggcgga ctttgctcat gccttgcaac aaccacaggg
     3781 actggtgctg gtaactggcc ctacaggcag tggcaaaacg gtcacctggt gttgtccacg
     3841 ctacacacca attccaccac tgaaacgctg gtacgtttac agcaaatggg ggtcgcccgc
     3901 tggatgctat catcggcgct tacgctggta atagcccagc gtctggtacg taaactttgc
     3961 ccacattgtc gccgacagca aggggagccc atccatattc cagacactgt atggccgtcg
     4021 ccgctgcccc actggcaggc acccggttgt gtacattgct accacggttt ttatggtcgt
     4081 accgccttgt ttgaagtcct gcccattaca ccagccatac gccagcttat ttccgctaat
     4141 actgaagttg aatcgctgga aacgcacgcc cgacaggcgg gtatgcgtac gctttttgaa
     4201 aacggctgcc tggccgtgga gcaaggctta accacctttg aagagttaat ccgcgtactg
     4261 gggatgccgc atggcaagta agcaactctg gcgctggcat ggcataaccg gcgacggcaa
     4321 tgcgcaagat gggatgctat gggcagagag ccgtacttta ctgcttatgg cactacagca
     4381 acagatggtt accccactaa gcctgaagcg aatcgccatc aattctgcgc agtggcgagg
     4441 agataaaagc gcggaagtca ttcatcaact ggcgacgcta cttaaagccg ggttaacgct
     4501 ttctgaaggg ctggctctgc tggcggaaca gcatcccagt aagcaatggc aagcgttgct
     4561 gcaatcgctg gcgcacgatc tcgaacaggg cattgctttt tccaatgcct tattaccctg
     4621 gtcagaggca tttccgccgc tctatcaggc gatgatccgc acgggtgaac tgaccggtaa
     4681 gctggatgaa tgctgctttg aactggcgcg tcagcaaaaa tcccagcgtc agttgaccga
     4741 caaagtgaaa tcagcgttgc gttatcccat catcatttta gcgatggcaa tcatggtggt
     4801 tgtggcaatg ctgcattttg ttctgccgga gtttgccgct atctataaga ccttcaacac
     4861 cccactaccg gcactaacgc aggggatcat gacgctggca gactttagtg gcgaatgggg
     4921 ctggctgctg gtgttgttcg gctttctgct ggcgatagcc aataagttgc tgatgcgccg
     4981 cccgacctgg cttatcgcgc ggcagaaatt gctgttacgc atcccgatta tgggttcact
     5041 gatgagggga caaaaactca cgcagatctt tacgattctg gcgctgacac aaagtgcagg
     5101 cattactttt ttacagggcg tagagagcgt cagagaaaca atgcgctgcc cgtactgggt
     5161 gcaacttctg acacaaatcc agcacgatat cagtaacggt catcccatct ggctggcgct
     5221 aaaaaatgcc ggtgagttta gcccgctctg tttgcaatta gtgagaacag gagaggcatc
     5281 cggctcgctg gacctcatgt tagacaacct cgcccatcat catcgggata acacaatggc
     5341 gctggcggat aacctcgcag ccttactgga accggcgttg ctgatcataa cgggaggaat
     5401 tatcggtacg ctggtggtgg caatgtatct gccaattttc catttaggcg atgcgatgag
     5461 tgggatggga taacgctggc gtgttgctcc acgccagcgt tgggagatta caggttgttg
     5521 aagatgcggt tttcttgttc cagcacacga ataaacgtgg tgcgcttggt cagctctttc
     5581 aggcgtgaag ccccaacgta tgtacaagct gaacgcaggc cgcccaaaat atcacgcgcg
     5641 gtattttcaa cctggcctcg cagcggcagc ttaacggttt taccttctgc tgcgcggtat
     5701 tccgcaacgc cgccaacgtg acgtttcatc gcagactcgg agctcatgcc gtagaacagc
     5761 ataaatttct cgccgttctc ctcaacgatg cgaccgccgc tctcttcgtg gcccgccagc
     5821 atgccgccaa gcatgacgaa atcggcaccg ccgccaaagg ctttcgccac atcgcccggc
     5881 gtggtacagc caccgtcgct gacgatcatt ccgcccagac cgtgcgccgc atcggcacat
     5941 tcgattaccg cagaaagctg cggataaccg acgcctgttt tgacgcgagt tgtacaaaca
     6001 gaacctgggc caatgccaac tttaacgata tcagcaccgg agaggataag ctcctcacac
     6061 atttcaccag tcactacgtt gccagcacaa atggttttgg tcggccacgc ttcacgcgct
     6121 ttcgcaacga actgcacgaa gtgttcggaa taaccattcg ccacatcaat acaaacgaag
     6181 tttaatgccg ggttcaggtc gagaatctgt ttagtttttt cgaaatccgc atcagacgtg
     6241 ccggtagaaa ccatcacatg tttcagcaca tcagcggaag aattgttgat aaacgcttgc
     6301 cactcttcga cagaaaagtg tttatgcaca gcagtcagaa tatcaaaaga agccagcgca
     6361 gaggccatag aaaatgtgcc tacggtgtcc atatttgcgg cgataatcgg cacgccggac
     6421 cagctctgac ctgaatgttt gaaggtgaat tgacgttcca gttcaacatc ggaacggctt
     6481 ttaagagtgg agcgtttagg gcggatgaga acgtctttaa aacctaactt cagatcttct
     6541 tcaatacgca tgtgcggatt cctggggtta atggctataa gggtaagagc gcaactccag
     6601 tgacgctatc atacgcacta atcaatgcgg cgcaagactg cgaaattgtc tttttttacg
     6661 ctacaatccc ataaatttac ctggtaaata gtgagcgaac gtttcatcaa tttcactttc
     6721 gcgcgctcat ttttaacgtt ttgataatca tgatgaattc ccgggaatat aaattatggg
     6781 gtatatagtt gccttaacgg gaggcattgg cagtggtaag agtaccgttg ccaatgcgtt
     6841 tgctgatctc ggaattaacg tcattgatgc cgatattatt gcgcgtcagg tggttgaacc
     6901 aggtgcacct gcgctacatg ccattgctga tcactttggc gaaaacatga ttgctgctga
     6961 tggaacattg cagcgccggg ccttgcgcga gcggatcttc gccaacccgg aagagaaaaa
     7021 ctggcttaac gacctgctgc atccgctgat tcaacaagag acgcaacacc agatccagca
     7081 agcaacttcc ccctatgtac tgtgggttgt gccattgctg gtagaaaact cactgtataa
     7141 aaaagcgaat cgagtgctgg tggtggatgt cagcccagaa acgcaactta agcgcaccat
     7201 gcagcgcgat gatgtaactc gcgaacatgt cgaacaaatc cttgctgctc aggcaacgcg
     7261 cgaagcccgc cttgccgtgg cagatgacgt cattgataat aacggcgcac cggatgctat
     7321 cgcatcggat gttgcccgcc tgcacgcaca ctatttgcag cttgcgtcgc agtttgtctc
     7381 acaggaaaaa ccgtaatgca gacccaggtc ctttttgaac atccactaaa tgaaaaaatg
     7441 cgtacatggc tgcgcattga gtttttgatt cagcaactca ccgttaattt acccatcgtt
     7501 gaccacgctg gcgcgctgca tttcttccgt aatgtcagtg aattactgga tgttttcgag
     7561 cgcggcgaag tccgcactga gctgttgaaa gaacttaacc ggcagcaacg taaactccag
     7621 acctggattg gcgtgcctgg cgtggaccag agccgtattg aagcattaat tcagcagtta
     7681 aaagcggcgg tgagcgtatt aatttccgcg ccgcgtatcg ggcaatttct gcgtgaagat
     7741 cgtttgattg ctctggtgcg tcagcgactg agcatcccag gcggctgttg cagctttgat
     7801 ttacctacat tgcacatttg gctgcatcta ccccaggcgc agcgcgacag ccaggtagaa
     7861 acctggattg ccagcctgaa cccgctcacc caggcactta ccatggtgct ggatttaatt
     7921 cgccagtcgg cccccttccg taaacaaacc agcctgaatg gtttttatca ggataacggt
     7981 ggcgatgccg acttgctgcg cctgaatctg tcgctcgatt cacagcttta tccgcaaatt
     8041 tccggacata agagccgttt tgccattcgt tttatgccgc tggacactga aaacggacag
     8101 gtaccggaac gtctggattt cgaactggcc tgttgctaag gagtaaaaat gtcagaaact
     8161 attacggtga attgcccaac ctgcgggaaa acggtggtgt ggggtgaaat cagcccgttt
     8221 cggccatttt gctccaaacg ttgtcagctg atcgacctcg gagaatgggc tgctgaagaa
     8281 aaacgaatcc ccagcagtgg cgatctttcc gaaagcgatg actggagcga agagccaaag
     8341 cagtgacatt tgtctgatgc ctcacgtagg cctgataaga cgcggacagc gtcgcatcag
     8401 gcatcttgtg caaatgtcgg atgcggcgtg aacgccttat ccgacctaca gacgcttaag
     8461 cttcgcaatt accggttcat tggctggcgg aaaatcatcg gcattaagac cgaccagcga
     8521 catccactca ccgggttgcc cttctttacc ccacggcccc ccttcccaac tttcgaccag
     8581 ccagaaccac agagttatat gcctgtccgg gaattcatat tccagttttt caaatagcga
     8641 aaaatgttgt ggggtaatcc cgacctcttc ctgaagttca cgcaccaccg cctgttccgg
     8701 cgtttcgccc atttcaattt taccgccggg aaactccagt ttattcgcca tgtgcgcatc
     8761 tgctgcgcga cgtgttataa agatttcatt gttctcgttg cgaataatac ctaccgcaat
     8821 ttgcagcttt ttcattgtct taaacctata aaaaaggcgc agaatcctgc gccttttact
     8881 tcaactgtta gcttttattg caggcgacca tggcactgct tgtatttttt accagaaccg
     8941 cacgggcaag gatcgttacg tcctactttg cgttcaccgg tttgcgccgc cagtgcagct
     9001 gcggctgcag agtcgtcatc ctgatggcta agctgctgca tttgtgctaa acgctcggct
     9061 tccatacgac gctgttgttc cagctcctca acctcttcag gcatacgtac ctgaactttg
     9121 ctcagcgtac tgataacttc atatttcaac gactccagca tcgctgcaaa catggagaac
     9181 gattcacgtt tgtattcctg cttcggatct ttctgtgcat agccacgcag gtggataccc
     9241 tgacgcagat agtccatcgc tgccaggtgc tctttccaca gggagtcaag cgtttgcagc
     9301 atgacgcctt tctcgaagtg acgcatcatc tcagcaccaa ccacttcttc tttacgctga
     9361 tacacttcga tggactgcgc cagaatgcgc tcacgcagcg tctcttcatg cagttctggt
     9421 tctttatcca gccactcggt aattggtaaa tcgaggtcga aatcgttctt cagacgttcc
     9481 tgcagccccg gaatatccca catttcttcc agcgactgtg gcggaatgta ggcatcaatg
     9541 gtggctttga acacatcttc acgaatgctg ttgatggttt cgctcacatc gctgacatcc
     9601 agcagttcgt tacgctggga gtaaatggcg cgacgctgat cgttagccac gtcatcatat
     9661 tccagcagtt gcttacgaat gtcgaagtta cgactttcaa ctttacgctg ggcgttggca
     9721 atcgctttag tcacccacgg gtgttcaatg gcttcgcctg gcttcatacc cagtttacgc
     9781 atcatgccgg atactcggtc ggaagcaaaa atacgcatca gcgcatcttc catcgacagg
     9841 tagaaacggg aagaaccagc atccccctga cgaccagaac gaccgcgcaa ctggttatcg
     9901 atacgacggg attcgtgacg ctcggtaccg atgatatgca ggccacctgc ttccagtacc
     9961 gcatcgtgac gtacctgcca gtcggcttta attttttcaa tttgctctgc ggtcggattt
    10021 tccagcgcgg caacttctgc ctgccagcta cctccgagca caatatctgt accacgaccc
    10081 gccatgttgg tcgcgatagt caccgcagcc ggataacctg cctgagcaac aatcgccgct
    10141 tcgttggcgt ggaatttggc gttcaggaca ttgtgcttaa taccggcttt ggtcagttcg
    10201 tttgacacca gctccgattt ttcgatggag atagtaccca ccagcaccgg ctggcctttc
    10261 gcagtacgtt ctttgatatc ttcaatgatc gcctgaattt tttccgcttc agtcatgtag
    10321 accaggtccg gcagatcttt acgaatcatt ggacggttgg tcggaacaac gacggtatcc
    10381 agtttgtaga tggagctaaa ttcgaaagct tcggtatcag cagtaccggt catccccgcc
    10441 agtttttcat acagacggaa gtagttctgg aaggtgatcg aagccagcgt ttggttttcg
    10501 ttctggatct gcacaccttc tttcgcttcc acagcctggt gcagaccatc ggaccagcgt
    10561 cggccctgca tggtacgacc ggtgtgttcg tcaacgatga taacttcacc atctttaacg
    10621 atgtagtcga cgtcacgggt aaacagcgca tgagcgcgca gcgccgccgt tacgtggtgc
    10681 atcagcatga tgttggccgg agagtacaga gactcccctt catccatgat gccctctttc
    10741 accagcagtt cttcaatcag caccagacca cgttcggtca ggttcacctg gcgagatttt
    10801 tcgtccaccg agaagtggcc ttcgccctgg aaggtttcgg agtcttcttt ttcctgacgg
    10861 atcaggtgcg gaataatttt attcacgcgt ttatacattt ccgagctgtc ttctgccggg
    10921 ccggaaatga tcagcggtgt acgcgcttca tcgatcagga tggagtccac ttcgtccacc
    10981 agcgcatagt gcagtttacg ctgtacacgt tcttcagggc tgaacgccat gttgtcgcgc
    11041 aggtagtcaa agccgtattc gttgttcgta ccgtaagtga tgtcagctgc gtaagcttcg
    11101 cgctttgccg gtgctggcat gcccggcagg ttgataccaa cagtcaggcc aaggaattca
    11161 aacagcggac ggttgttttc ggcgtcacgt tgcgccaggt agtcgttgac ggtaactacg
    11221 tgcacgcctt taccggttag tgcgttcagg taagcaggca gcgttgcggt cagggtttta
    11281 ccttcaccgg tacgcatttc ggcgatgcag cgttcgttaa gaaccatacc gccgagtaac
    11341 tgaacgtcga agtgacgcat accaaagacg cgcttacttg cctcacgcac cacggcgaaa
    11401 gcttccggga tcagattttc cagcacttcg cctttttcca gacgcgcacg aaactctgcg
    11461 gttttccctt tcagttcttc atcggagagt ttttccatct ccggttccat ggcattgatg
    11521 atgttgacca ctttgcgcat ccggcgcagg gtgcgatcgt tacgactacc gaaaacttta
    11581 gttaacaatt tgattagcat aataaaatct caaacgcccc gcgttacgga gttaataaaa
    11641 tgaagtaaag gtttattgtt gttaggtgag gcgttgaggg ccagcacgga tgccttgcgc
    11701 ctggcttatc cagacgggcg tgctgaattt tgcttgtggg gtaaaatgcg cataatcaat
    11761 gcgataaccc ttttcagacg gcgtgccttc ctgggtcagc agcgcgctga gcgtatccag
    11821 taatgcaaga tgttgcgcct gaagaggcaa agattcttca gcaacgggca gtgtttgcgg
    11881 tgccattgcg aaagaaagat gacggattac cgtacgaata gcatgttgat gccagtaatc
    11941 aacggaatag ttcgaattcg ggcggcgcgt gttcgcttcc agcaaggcca attgaccaaa
    12001 gttaactttg gctgaaggct cgtggttgcg ggttgtcgct tttgcgggcg cgtttggttc
    12061 ggcggcgttg ctgagcgcag gcaaacctaa actcgccgcg accatcccta ataagagatg
    12121 cggccagaag tagcgtttac caaactgtcg ccagcgcgtc agtattccac tcacgttatt
    12181 gccatcccga tcgaattcaa acgtatttag cgcacaaatc ttatcattaa agcctgaagt
    12241 ccacagcacg aatgaagata agttgtgcgt aaaagtgctt aagaaaacag cgttcgcacc
    12301 gttttttata gctcttataa aattcaaaaa aaaacgactg gttcacctgg ccggagagag
    12361 tgccagattt gccagtcgaa ttttatacga cagtataaat gtcgttatgc cagtacagct
    12421 gaaggcgctt tgaaggccaa cggcagttct gcgtcgtcct ggaaggtcac atattcccag
    12481 gcttcctgtt tcgccaggac agcctgcagc agtttgttat tcagtgcatg accggattta
    12541 taagcggtaa atgcaccaat aatattgtga ccacacatga acaagtcacc gatcgcatcg
    12601 agcattttgt gacgcacaaa ttcgtcttca aaacgcaggc cgtcttcgtt cagtacgcga
    12661 taatcgtcaa caacgatggc acaatcgaag ctgccgccca ggcacaaacc acgggactgc
    12721 agatattcga tatcacgcat gaaaccgaac gtacgcgcac ggctgatctg gcgcataaac
    12781 gcatcagcgg agaagttcat cgcatagcgc tggttgctgg aatcaatagc cggatggtta
    12841 aaatcgatgg tgaaatccag cgaaaaacca ttgtacggct taaattcagc ccacttatcg
    12901 ccatcttcga cacgaacagt ctctttgatg cgaacaaatt ttttggcgca gttcaactcg
    12961 tcgataccgg cgtcaagcag caggtataca aacggagcgg cgctgccgtc catgatcggg
    13021 atttccggcg cgttaacttc gataacaatg ttatcgatgc ccaggcccgc gagagcagca
    13081 ttgaggtgct ctacggttga aatccgtaca tcatgctcgt tgaccagaca cgtacagagc
    13141 atggtatcac gcacagattt ggcatcggcc gggaaatcta ccggtggatt caagtcggtg
    13201 cgacgataga tgaccccggt gttggccggc gcagggcgta acgtcagggt gactttcttg
    13261 ccggtatgta aaccgacacc cgtcgcctga acgatacgtt taagtgtcct ttgtttgatc
    13321 atcgtattat ctcgccaaat tacctatcca accgaagtgt actatacatt cggcgggcca
    13381 gtttagcaca aagagcctcg aaacccaaat tccagtcaat tcttaatcag cttgcttacg
    13441 taggaatgct gggatatcca gataatccgg ctctttcgca gtttgcggcg cattgtcatt
    13501 cactacttta gcaaccggct tctgctcctg ggtcagcgga gccatcccat gctgctggta
    13561 gcgatccatc actggctgct gaacctgctt attggtcacc agagtgattt caggacgttt
    13621 gtccatgccg atacctgtcg caacaacggt tacgcgcagc tcgtcattca tatccgggtc
    13681 aagagaagta ccgataacca cagtcgcgtt gtcggaagca aatgcacgga tggtgttacc
    13741 taccgtttcg aactcatcca gacgcaggtc gaagcccgcc gtgatgttaa ccagcacgcc
    13801 gcgcgcgcca gacaggtcga tatcttccag cagcggagaa gagatagcca tttcagcagc
    13861 ttcttccgca cggtcttcac cgctcgccac gccagaaccc atcattgcgt agcccatttc
    13921 agacattacg gtgcgtacgt ctgcaaagtc cacgttcatc aaacccggac gagtaatcag
    13981 ttcggcgata ccttgcacag cgcctttcag tacatcgttc gctgcgccaa acgcatccag
    14041 cagggagata ccgcggccca gaactttcag cagtttgtcg ttcgggatag tgatcagaga
    14101 gtccacatgc ttggacagtt cagtgatccc ctgctcggcg aatgccatac gcttcttgcc
    14161 ttcaaagttg aaaggcttag tgacgacagc aacggtcagg atacccaaat cttttgccac
    14221 ttcagcgacg actggtgctg cacctgtacc ggtaccacca cccatacccg cagcaataaa
    14281 gaccatgtct gcaccttcca gcgccgcacg caatgcatcg cgatcctcat cagccgcatt
    14341 gcggccaact tctggattag cgccagcgcc cagtcctttg gtgataccgc taccgatttg
    14401 aatcgtctgt ccaaccgctg ttttacgcag cgcttgtgca tcggtattta ccgcgaagaa
    14461 ttcaacacct tcaatacgct cgcgcaccat gtgttcaaca gcattaccgc cgccgccgcc
    14521 gacgccgatg actttaatca ccgcgtcatt ggtaagttcc attggttcaa acatagtttc
    14581 tctccgattt gtgcctgtcg cctgagtccg taatcatcgt cggcctcata aaaattaaaa
    14641 ctcttttcgc agccaactat tgagtcgctt gatccacgag ccaactgatg ctgtaacacg
    14701 tttttctact tcagcttcac cgttaagatg tgactctttc ccatagtgaa gcaatcccac
    14761 cgccgtcgaa taatacggct cctgagcata atccgttaaa ccggtaatgt tcagcggcgc
    14821 gccgatacgc acttgcgtat gaaacacgcg ctgagcacag gctgcaagac cttcgatctg
    14881 cgctgcgcca ccggttaata caatgcctgc cgccaggtga tgtttaaccc cttgttggcg
    14941 aagcttttcc tgcaactgca atatctcttc gttgaccagg ttgagcaact cggtatagcg
    15001 cggctcgatc acctctgcca gggtctgacg ttgcagactc cgcggcggac gaccgcctac
    15061 gctcggcact tccacgctct catcttttcc aacgatggaa cccagcgcac aaccgtggcg
    15121 aactttaatc gcttcggcgt cgcttggcgg cgtgccaaag gcgtaagcga tatcactggt
    15181 cacgacattg ccagcataag gaattacctt tgtgtggcgc aatgccccac cggtataaac
    15241 ggcgatatcc attgtaccac caccgatatc gacgacgcag acacccagtt cacgttcatc
    15301 ttccgtcaat accgaataac ttgatgccag tccggcaaat atcagttggt caactttcag
    15361 cccacaacgt tcaaccgctt tgacgatgtt tttcgccata tcgttgtgac atgtgatcag
    15421 gtgcactttt gcctgcatcc gcacacccga aagtcctacc ggattcttga tcccttcctg
    15481 atagtcaatc gcatactctt gcgggatcac atgcagcaca cgatgctcat cgcgcacacg
    15541 caccgatttc gcggtatgga cgacgttttc cacatcttct tgcgtcactt cttcttcaga
    15601 aataggcacc ataccaattt cattctggca gctgatgtgc ttaccagaaa gcgccagata
    15661 taccgaagag atctgacaat ctgccatcaa ttctgcctgg tcaatggcgc gttgtacgca
    15721 cttgaccacg gattcgaggt cgttcacccc gcctttatcc ataccacgtg acgggcagct
    15781 gcccacgcca atgatattga ccataccgtc gggcagaact tcccctacta aagcggcaac
    15841 cttcgcggta ccaatttcca gtcctactac cagttttctg tccgtcgcct tgatcattgt
    15901 tgttctgcct gtgcctgatt ttgttgctga gtagattcct ctggcggcaa gggcgcccag
    15961 cctactgccg ctccagagtc ataacgcaaa tcaacgtagc taatccgttt gccatcggtt
    16021 tgcgcctgct gctgtaaaac cggataaagt tctacaaagc gaaccaaacg tttcatcgta
    16081 tcgccccggc caagattgag cttaatatcg ttattcagcg tcaactgcca ggaacgccgc
    16141 gcggtcatcg ccgcttcctt cagagtaaat ctgtcctttg ccagcatctg ccccatttcg
    16201 cgatagccct gcaacacttc attagcgctg ccttccgggc catacagcat tggaagcacc
    16261 tgcttgctgg tgcgatctgg cggcacgctg aaggtgtttc cttccgcgtc taccatgtgt
    16321 tgatcattcc accgcgcaat cggcacatat tcaaccagat gaatcttcaa ttcatcaggc
    16381 cactgctttc tgacgctcac ctgcttaatc cacggcaggc gttgttctat ttgcgtctgg
    16441 atgatgttga catcctgggt cataaaggta cccggctcac ccaatgccag gatcgactgc
    16501 cggatatcgt cattacgcgt gtaatggcgt tcaccggtca acaccagctt tgagagcggc
    16561 aggcgttgcg catcttccat ccagctcaac acgacccagc cgctcaccaa cactgtcgtt
    16621 aaaacggtca gcaggaaaag gatccccgcc agacgcgttc cattattgcg gcgagaagaa
    16681 acctcttctt cgctgtttcg cgtgttcaga gcagcctgcg acatattagt ccgccagttc
    16741 cagaattcgt actaccaact gcgagaagct catacctgcc tgacgtgccg ccatcggcac
    16801 caggctgtgg ctggtcatac ccggtgaggt attggcttcc agcagataaa actgtccatc
    16861 actgtccagc ataacgtcaa tacgtcccca tcctttgcaa cctaacgtcg tccatgcttt
    16921 cagcactaat gcctgcaaat tggcctcttg tgacgcttcc agacctgcgg ggcagaaata
    16981 ctgtgtctca tcagagagat acttcgcctc ataatcatag aaggttccgg acggttgaat
    17041 acgtattgac ggtaaaattt cttcaccgag tatcgcaacc gtgaactccg gcccacttag
    17101 ccatttttca atcaatactt cttcatcgtg ctgaaatgcc aatcttaatg catcttgtag
    17161 agcattttct gctactactt ttgacattcc cacactggaa ccttcgcggc tcggcttaac
    17221 gataaccggc aaacccagag cagaaatttc tgctaactgc ttatcgctca ggcctttttc
    17281 aaactctgcg cgggttaacg ctacccacgg cgcgaccggt aaaccggcac cttgccacag
    17341 aagtttgctg cgtagtttat ctattgaaag cgcagatgcc atcactccgc tgccggtata
    17401 aggcaagccc atcagctcga gcatgccctg cagcgtacca tcttcaccgc cgcgaccgtg
    17461 tagcgcgata aacactttct gaaaacccat cgacttcagt tgcgtcacgt cgacttcttt
    17521 cgggtcgaca ggatacgcat caataccgcc ttcacgcagc ccggctaaca ccgctgcgcc
    17581 agaattcaga gaaacttccc gctcagcgga agtcccgccc aacaggaccg cgattttatc
    17641 agtcatgttg ttcttcctcc ggagtttgcg gcttcagttt gatttcagct aaagaacggg
    17701 caatttttcc gatattacca gccccctgaa cgagaatcag gtcgttaccg gttaataccg
    17761 gtgccagcat ctcggctacc tgcgcagggt ccggcaccag aatgggatca attttcccac
    17821 gtccacgaat tgtgcgacac agcgaacggc tgtccgctcc cggaattggc gcttcgccag
    17881 ccggatacac ttccagcatc aacagggtat caacctgcgt cagcacattg gcgaaatcat
    17941 catacaggtc gcgcgtacgg gtaaaacggt gcggctgaaa cagcattacc aggtttttat
    18001 ccggccagcc tgcgcgcgcc gctttaatgg tggcgtcaac ttccgtcggg tggtggccgt
    18061 agtcatcgac cagcattgcc gtaccgcttt taccattcac tggctccagc gggaattcac
    18121 cgaggaaatc aaaacggcga ccagtcccct ggaagctctc cagcgcacgc aaaatagcct
    18181 cgtcgtcaat tccctcttcc gtagcaaccg caaccgcagc tgcggcgttc agcgcgttat
    18241 gacggcctgg cgcattcagg gtgacgcaca tcggctcttt gtcctgacgc agcagcgtaa
    18301 agtgcccctg cgggccaatc tgctgataat cttctacacg cacgtcggca tcttcgctga
    18361 agccgtaagt tgtggtctga cgtcccacac gcggtaacaa ttcgcggatc accggatcat
    18421 caacacacat caccgcacga ccgtaaaaag gcaggttgtg cagaaaatta ataaaggtct
    18481 gttttaaatt ctcaaagtcg ccctggtagg tatccatgtg gtcggcttcg atattggtga
    18541 caatcgccac catcggttgc agatgcagga acgatgcatc actctcatct gcttcggcaa
    18601 tcaggtaccg accatgcccc aaacgcgcat gaacccccgc cgcttttacc agcccgccgt
    18661 taacgaaggt tgggtcgagc cccgcttctg cgtagatgct ggaaaccatc gcggtagttg
    18721 tcgttttgcc gtgcgttcct gcaatggcga tgccatgacg aaaacgcatt aactcagcca
    18781 gcatttcggc acgacggatc accggaatac gcgcttcatg agctgcgaca atttccgggt
    18841 tgtcggcaga aatggcgctg gaaacaacga ccacgctggc atcacgtacg ttttccgggc
    18901 gatggttgaa ataaatcgtc gcacccagat tcagtaactg ctgcgtgacc ggatttggcg
    18961 ctaaatcgga accactgatc tgataacctt cattggccag aacttcggca ataccgccca
    19021 taccggcacc accaatgccg acaaaatgta tgtgccgaac gcgacgcatt tcgggcacga
    19081 tggaacgcag ttttgccaat tgttgtgtat tcattcttta cgccattaac ttcttaaatt
    19141 catacgatgc aaaaggcatc gctacaatta cgcccgggca gcccggctca cttcatttgc
    19201 cactcgctcg gtggcatccg gaatggatgc agcgcgggcg cgttctgcca tggttaataa
    19261 ggtttctcgc gaccacccgg ccagggtgtt ggcgacagca tccacgctaa gctgtggctg
    19321 ctcgataatt ttggctgcgc ccgctttttc cagcggtagc gcattccagt attgctggcg
    19381 gtctttatgt tgaaacggca caaacaacgc cggtagtcct gccgcggcga tttcactcac
    19441 agttaaagca ccggagcggc aaacgacgac atccgcccac gcatacgccg ccgccatatc
    19501 atcaataaat tcagtcactt tatgctgcgg ttgccccgct tcggcatacg cctgttcaac
    19561 ggattgttgc gaacctttgc cgctctgatg ccagatagtg actgaatcac ccagtttcgc
    19621 agcaacctgc ggcattgtct ggttaagaat gcgtgcgccc tgggaaccac ccactaccaa
    19681 cacacgaacc ggaccttcac gtccagccaa acgttgctgc ggcaacggca gcgccagcac
    19741 atcggtacgc accgggttac ccaccacttc cgcattaggg aaagcgcctg gaaacgcctg
    19801 catcactttg gtagcaatct tcgccagcca tttattggtt aagcccgcaa taccgttttg
    19861 ttcatgaagt acaaccggaa tgcctaacga ccacgcggcc agaccacctg gacctgatac
    19921 atagccgccc ataccgagta ccacgtcagg tttgtacgct ttcataatcg cccgcgcctg
    19981 acgccaggcg ttgaagatac gcagcggcgc agctatcagt gcttttatac cttttccacg
    20041 caaaccagag atacgaatga aatcaatttc gatgccatgt tttggcacta agtccgcttc
    20101 catacggtcg gcagtcccca gccagcgaac ttgccaaccc tgagccatta gatagtgcgc
    20161 aaccgccagt cccgggaata catgtccacc ggttccgcct gccatcacca ttaatcgctt
    20221 tccttgacca ctcatcgtga acctcgtaca aacgcctgcg ctttctccag acgcgtttca
    20281 taatcaatac gcaacagcat catgatggct gtcgacataa tcagtaagct cgaaccaccg
    20341 taactgatta gcggcaatgt cagacctttg gtcggtaaca tccccgccgc cgcgcctacg
    20401 ttaaccagcg cctggaagct aaaccagatg ccaatagaac aggcgagaaa accggaaaaa
    20461 cggtggtcaa tttctaatgc tttgcggcca atcgacatcg cacgaaaagc gacgaagaat
    20521 accattaaaa gtgctagtac cacaccgaca taacctaatt cttcgccgat aatggcgaaa
    20581 ataaagtcag tgtgcgcttc cggcagatac tccagttttt gtaccgaatt acctaaacct
    20641 tgcccccaaa gttcgccgcg accaaacgcc atcagcgatt gcgttaactg atagccgctg
    20701 ccaaagggat cttcccacgg gttccagaat gcggtaacac ggcggatacg gtacggttcg
    20761 gcgaggatca gcaacacaac cgctgaaatg cccataccga taatggcaat gaactgccac
    20821 aatttcgctc ccgccaggaa caacatcgcc agcgtagtca caaacaacac caccaccgta
    20881 ccaaggtctg gctgtgccag cagtaacact gccaacacca gaatcacgcc catcggtttc
    20941 aggaagccgc gcaggttatt acgtacttcg tcgcctttac gcaccagata gttggcgata
    21001 tagcaaaaca gcgacagttt tgtcagctcc gcaggctgga tacgcagcaa accgagatcg
    21061 atccaacgcg atgccccttt aacagcgcta cccactacca gcacgatcat cagcaggatg
    21121 atagatccga gcagcatcgt ggcactgtag cgttgccaga actccatcgg cagacgcagc
    21181 gtaatgatcg ccagaataaa cgccaaaatc agatagacac catcacgctt cgcgaagaag
    21241 aacggatcgt tggttaagcg ttgccctatg ggcattgacg ccgaggtcac catgataaag
    21301 ccaatcgccg cgaggccgaa ggtcagccac agtaaggtgc gatcgtacat gatcaggctg
    21361 tcggtatctt tttcccgcga gcccatcacc cagcccttta gcgccgtgga gatccagacc
    21421 aggatactga atcctggcag gcgcggcatt ttcaggcgag ggagagataa acgcatcaac
    21481 ctaactcctt cgccagacgg gcaaactcat tgcctcgttg ttcaaagttc ttgaactgat
    21541 caaggctggc acaggccggg gagagcagaa ccatatcgcc cggctgaaca cgcggagcca
    21601 gcaagcgcat agcctgttcc atagtttcgg tttgttctgc cacttccggg cgtagcgccg
    21661 ccagctgcgc gccgtcacga ccgaaacaat acagacgtac gttatcgcca ttcaggtaac
    21721 gcaccagtgg gctaaagtcc gccgatttac catcgccgcc cagcaacaaa tgcagcgtgc
    21781 cgtctacgtg caggccattc agcgccgctt ccgtactgcc gacgttggtc gcttttgaat
    21841 cgttaatcca gcgcacaccg ttatgctcca gcaccacttc aaagcgatgc ggcagaccgg
    21901 tgaaggtggt taacgctttc aggctgctgg cgcgcggtaa ccctgcggca tctgccagcg
    21961 ccagcgccac cagcgcattg gtgtagttat gctgcccgga aagtttcatc tctttcacat
    22021 tcagcacttt ctcgccttta acccgcagcc aggtttcgcc ctgctgatga ttcaggtgat
    22081 agtcacccat gttgacgcca aagctgacgc agcgttcatc cgcaccgcga atcggcattg
    22141 ttaaggcatc atcagcatta accacgcaaa ctttcgcgtt ttcgtaaatg cgcagttttg
    22201 ctgcacgata ctgttgtaaa ccaaacgggt agcgatccat atgatcttca gtcacgttca
    22261 gaatggtcgc tgctaccgcc tgtaagctgg aggtggtttc cagctggaag ctcgacagtt
    22321 ccagcacgta cagttcacac tcatcatcca gtagcatcaa cgcaggcagg ccaatattgc
    22381 cacccacacc aacgttaacc cccgccgctt tcgccatttc acccactagc gtggtgaccg
    22441 tgcttttgcc gttagaaccg gtaatcgcca caatcggtgc ttgtgcttcg cgacaaaaca
    22501 gctcgatatc gccaacgatt tcgattccgg catcagcggc agcgcttaag gatggatgcg
    22561 ccagtgcaat accgggactg gcgacaatca gatctgccgc catcagccat tcatcattca
    22621 gactgcccgt gtggcgttct acggcttcgg gtaatttatc caggccaggc ggtgtcatac
    22681 gcgtatccat aacgcgcggc gtcacaccgc gagcgaggaa aaagtccacg caggaaagcc
    22741 cggtgaggcc caggccgata atgacaacat ttttaccctg gtaatcagcc atgattaacg
    22801 taccttcagc gttgccagac caatcaggac cagcatcagc gaaataatcc agaaacgcac
    22861 aatgacgcgc ggttccggcc agcctttcag ttcatagtgg tgatgaatcg gtgccatgcg
    22921 gaaaatacgt tgtccgcgca gtttaaagga gccgacctgc aggatgacag aaagcgtttc
    22981 taccacgaac acgcccccca taatcaccag caggaattcc tgacgtagca gtacggcgat
    23041 aatgcctaac gcaccaccta acgccagcga accgacatcg cccataaaga cctgcgccgg
    23101 ataggtgtta aaccacagga agcccagtcc tgccccgact atcgcggtac agacaataac
    23161 cagttccccg gcgtgtcgca gatacggtat gtgtaaatag ctggcaaagt tcatgttgcc
    23221 ggtcgcccac gccaccagcg caaaaccacc ggcgacaaat acggtcggca taattgccag
    23281 accgtcaaga ccatcggtca ggtttaccgc gttgccagta cccacaatga cgaagtaagc
    23341 cagcagaatg tagaacagcc ccagttgcgg catcacatct ttaaagaatg ggaccaccag
    23401 ctgcgttgcg ggcgtgtctt tgccggcaag gtacaaagcg aaagcaacac ccagcgcaat
    23461 gaccgacatc cagaaatatt tccaacgagc gatcaaccct ttggtgtctt tacgcaccac
    23521 tttgcgataa tcatcaacaa agccaataac accgtaacct accagcacca ccaacacgca
    23581 ccagacgtac ggattggacg ggtaagccca cagcagcacg gagatcacaa tcgctgtcag
    23641 gatcataatc ccgcccatgg tcggcgtacc gcgcttgctg aagtgtgatt caggaccgtc
    23701 gttacgcact acctgaccaa aggaaagttt ttgcaaatga gcaatcatgc gcgggcccat
    23761 ccacaatgag atgaacagcg cggtcagcag gctgacgatg gcgcgaaacg tcagatagga
    23821 aaagacgtta aagccggaat aatatttgac caaatgttcg gccagccaaa ctaacatgtc
    23881 ccattctcct gtaaagcgcg tactacctct tccatggcag cactacgtga acccttaact
    23941 aaaatcgtaa ttacctgttg ctcagcaatc agtgatttaa gacgcgtaat taacgcagtt
    24001 ttatcagcaa aatgttcgcc aacgccgctg gcggtgctga tagcatggct ttgtttaccc
    24061 atgcttaaca cgcggtcaat accagcagct tttgccgcct cacccacctg tacatggcag
    24121 gcttcgcttt cagcgcccag ttccgccata tcgcccacca ccagcacgcg gtagcccggc
    24181 atttcagcca gtacctgaac agctgcggtc attgaaccga cattggcgtt gtaggagtcg
    24241 tcgagcagca actggttttc tgccagttgg atggggaaca gacggccagg aacagctttc
    24301 agatttgcca gccccgcttt gatagcatca agcgttgcgc ccacggacat ggagagcgca
    24361 gcggctgcca gcgcattcgc aatattgtga cgccccggca acggcagcag aacatcgacg
    24421 ctaccggttg gggtttgtag ggtaaattcc ataccgtgcg aggtcacatg gatattggtg
    24481 gcggtgaaat cgctgttggc ggcattgggt gagaaacgcc acactttgcg tgagccaatt
    24541 acgctctgcc agttcagcca gtcgttgttg tcggcgttca taatggcgat accgttttcc
    24601 ggcaggccgc taaagatttc acctttcgct ttcgcgacac ccgcaagcga gccaaaacct
    24661 tccagatgcg ccgctgccag gttgttgacc agcgccgctt ccgggcgagt cagactcaca
    24721 gtccaggcaa tctcgccctg atggttcgcg ccaagttcaa taactgcgta atcgtattcc
    24781 ggcgttaagc gcaacagcgt catcggtaca ccgatgtcgt tgttgagatt gcctgccgta
    24841 taaagcgtgt tgccgcactg gcttaaaatc gccgccgtca tctctttaac ggaggttttg
    24901 ccggaggacc ccgtcagagc aaccacgagc gccggaactt gctggcgaac ccatgcagcc
    24961 agttcaccaa acgccagacg cgtatccttg acgattaact gcggcaggtc gatgtccagc
    25021 ggacggctaa ccagtagtgc gcctgcgccg ccagctttcg cctggtcggc aaaatcgtgg
    25081 gcatcaaaac gttcaccttt cagggcaaca aacaggcagc ccggcgtcag ttttcgggta
    25141 tcagtggtta cagcatcaag ggtgatatct gcaccttgca gttcaccgtt gagaatatcg
    25201 gtaagttggc taagggttac gctaatcatg caatcccccc cagcagacgc gccaccgtga
    25261 cgcgatcgga gtagtccaga cgctgattgc caacaatctg gtaatcctca tggcctttgc
    25321 ccgcgaccag taccacatca ttctctttag cctgcataac ggcgcaagtc accgcttcag
    25381 cacggccttc catcactttg gcatgtccgg catctaacat tcccgccaga atatcgttga
    25441 tgatggcacg cggttcttcg gtacgcgggt tatcgtccgt caccaccgcc acgtcagcaa
    25501 actcttcggc aattgcgccc atcagtggac gcttaccttt atcgcgatcg ccaccacagc
    25561 caaagacgca ccacagcttg cccgcacagt gcaggcgcgc cgcctgtaag gctttttcca
    25621 gtgcatccgg cgtatgcgcg taatccacca ccaccgtcgg tttgcctggc gcagtgaaca
    25681 cttccatacg tccgcaaacc ggttgcagac gcgcggcggt tttcagcaga tcagccagtg
    25741 gatagcccag cgccagcagt gttgccagcg cgagcaacag gttgctgacg ttaaaagcgc
    25801 ccatcagatg gctttcaatt tcgccatcgc cccagcttga actaaagcga atggtcgcgc
    25861 cgctgtcgtg atagttcact tcggtcgctt tcaaccagcg tccgtgacag ttcggattga
    25921 tatgatcttc cattgatacc gcaaccgcgt ccggcagttt tgccagccag cggcggccca
    25981 cttcatcgtc ggcgttaata atcgcctgac cgcaatgatg ttcagaataa agcagccatt
    26041 tcgcagcttc gtagtgttcc atatcaccat gataatcaag gtgatcgcgg cttaagttgg
    26101 taaagaccga cgccggaaat ttcaatgccg ccacacggtg ctgtaccagc ccgtgggagg
    26161 aaacttccat tgcgcaaaaa gtcgcgccct gatccaccag ccccgccagc tcatgctgaa
    26221 catcaactgc cgaaccggta gtattttctg tcgggatcac tttccccagc agaccgttac
    26281 caacggtgcc cattaccgcg ctggtttcgc caagcagttg gctccactgc gccagaagct
    26341 gggtcgtcgt ggttttgccg ttggtgcccg ttacgcctac gagacgtaaa ttgtcagagg
    26401 gttcatggta aaagcggccc gccagtgcag ataaacgctc gttgagctgg ctgagataga
    26461 tgaccggtac gccgtgcatt tcacggattt caccgtcggt cgcctcatct ttcgcctctg
    26521 caataatggc agccacacct tgcgctatcg cctgcgggat atatcgacgc ccgtccgcct
    26581 gatgacctac tacagctaca aagagatcgc ccgccgcagc cacacggctg tcgagtgtca
    26641 tctctcgcag tgctcgcgaa ggtgcgtctg gcacccacgg agcaagaagg tcgcgcaaat
    26701 tacgatctgc cacctgtccc ctcgccttga ttaatcacaa attcattttt atcgcccgtt
    26761 gtcagcgcat ccggctcgat gttcatggta cgcaatacgc cgcccatgat ggcaccaaag
    26821 accggcgcgg aaacggcgcc gccgtagtat ttacccgcct gcggatcgtt gataacaaca
    26881 accagcgcga agcgcggctg actcgcaggc gcaacgcctg cggtataagc aatatattta
    26941 ttgatgtagc gaccgtccgg cccgaccttt ttcgcggtac cggttttaat ggcgatacga
    27001 tagcctttaa tcgccgcctt cacgccgccg ccgcctggta gcgccacgct ttccatcata
    27061 tgcaccacgg tgcgaacaat ggattccggg aagacacgtt caccgggaac cggggggtca
    27121 actttggtaa tcgacagtgg gcgataaatg ccgtagctgc cgatagttgc gtagactcgc
    27181 gctaactgta acggtgttac cattagcccg tagccgaaag agaaggtggc cctctctatg
    27241 tcagaccacc gttgtttttg aggatataag ccactgcgtt ctccgaccaa ccccaaattg
    27301 gtcgcttttc ccagtccaaa acgtgagtaa gtatctacta acactgagga cggcatcgtt
    27361 aacgccagct tggaaacacc gacgttactc gacttctgta ataccccggt cagggttaat
    27421 tcgctgtagc gtgccacgtc tttgatttcg tggccgttaa ttcgataagg aatggtattg
    27481 agtaccgagt tttcccgcac cacgccacgt tgcaacgcgg tcatcaccac catcggttta
    27541 accgttgagc ccggttcaaa cacgtcggtg atggtacggt tacgcatcgc ctctttcggc
    27601 gtgccgctca gattgttagg gttgtatgac gggctgttag ccatcgccag cacttcacct
    27661 gtgttgacat ccaccagcac ggcgctacca gattcagcct tgttaaaggc caccgcgttg
    27721 ttcagttcgc gataaaccag cgcctgcagg cgttcatcaa tactcagcgc caggttgtgc
    27781 gctgcctggc tgtcagtaga agaaatatct tcaattacgc gaccatagcg gtctttacgc
    27841 acaatgcgct cacccggctg cccggtaagc catttatcga aactcttctc aacgccctca
    27901 atcccttgac tatcgacgtt agtaaagccg atgaggtgag cagtcacttc gccggacgga
    27961 tagtaacggc gagactcttc acgcagatga atccccggca gtttcagttt tttgatgtag
    28021 tccgccatgt cagagtttac ctgacgcgcc agataaataa agcgcccttt cgggttggcg
    28081 ttaatgcggg cagaaagctg atccagcgga atattgagcg cgttagccag cgccttccag
    28141 cggtcaccga cgctgatacc gccagcgtca tgcacttctt tcgggtcagc ccaaatcgct
    28201 tttaccggca cgctcaccgc taacgggcga ccagaacggt cagtaatcat gccgcgggag
    28261 gtggaaactt gctgaacgcg aagagaacgc atgtcgccct ctttcaccag catatccggg
    28321 gagataactt gtaaccacgc tacgcgtccg agcagaaaag ccagcgccag gagaatacag
    28381 ccgcataaca acgcaaaacg ccaactgata aagttggcat gttcttcctg acgttttggt
    28441 ttctgcgttt tcgccgctgc tttcatgcgt cgcgtttatc cttatttttg cactacgata
    28501 ttttcttgtg acggatcaac atgctgcatt tgcagctttt ccgtggcgat cctttccacc
    28561 cggctatggt cgccgagcgc attctcttca aggatcaggt tgcgccattc aatgtctaaa
    28621 gcatctcgct ccagcaccag ttgttcgcgc tgagcggtca gtaaacgggt atggtgcgcc
    28681 gtggttacca cggtcaccgc cgtcaaaata atgcaaatga acaggcagag tggcagcttc
    28741 ccaaatcgca aaagatcgtc accgataaca ccaggcaatg catggcgctc gtggcttccc
    28801 atcgatcctt taactttgct tagagcttct gtcactctgc tgatcatgcg ttcgtcctct
    28861 ctgcaatacg cagaactgaa ctacgggcac gagggttctc agccacctct tcttcgcccg
    28921 gcattaactt gcctagtgct cgcagctgac ggccacccag ttttttgagc tgctcttcag
    28981 tcatcggtaa ccctgccgga acttgcggac cgcggctgtt ttcacgcata aaacgtttca
    29041 caatacggtc ttccagcgag tggaagctga tgatcgaaag ccgcccaccc ggggccagca
    29101 cgttgagcga gctttttagc gcctgctcta tctcctccag ttcactgttt acccaaatgc
    29161 gcaccgcctg gaaggtacgg gtcgcgggat gtttaaactt atctttcacc ggcgttgcag
    29221 cagccacgac ttccgccagt tctttggtgc gggtcatcgg ctgttcgcgg ttacgctcga
    29281 caatggcgcg ggcaatgcgt ttggcaaaat gctcttcacc ataggttttc aatacccagg
    29341 cgatatcggc ttcttctgcg gtttgtagcc attcagcggc tgactgccca cgggttgggt
    29401 ccatacgcat gtccagcgga ccatcgcgca taaaggaaaa gccgcgttca gcatcatcaa
    29461 gttgcggtga agagacgcca agatcgagga gaatgccgtc gatcttgcca ataagatcgc
    29521 gctcggcaac gtattcgccc agcgcggaga aaggtccgtg gatgatggag aagcgcggat
    29581 catcaatagt cttcgcaacg gcgatagcct gcgggtcgcg atcgatcgcc agcaaacgcc
    29641 cctcttcgcc aagctgcgag aggatcagac gtgagtgacc accgcgacca aaagtcccat
    29701 caatgtagat gccatcagga cggatattga ggccattaac ggcttcatcc agcagcaccg
    29761 tagtatgttt atagttttcc atcattttat agagacaagt cctgcagtcg ctccgataag
    29821 tctccggtag ccaactgctc tgcgtcgata tcttccttga cctgttgatg ccaggttgtt
    29881 tcatcccaca gctcaaactt gttgaactgt ccaaccagca tcacttcttt tgtcagcccg
    29941 gcatgttgcc gcagtactgg cgcgattaac aatcgacctg cgccatccat ctgacattcg
    30001 ctggcatgac ctaacagtag gcgctgcaca cggcgctcaa ccgggttcat gctcaacaga
    30061 cgcgataatt tttgctcgat aatttcccat tcaggcaggg ggtaaagcag caggcacggg
    30121 tgatgaatgt caatggtgca aaccatttga ccggcagcgt tctcaagcag ctgttcccga
    30181 taacgggtag gcactgataa gcgccctttg ctgtcgagat tgactaacgt tgctccccgg
    30241 aacatgccag cctcacccct tattcccact ttgccccaca atttcccact gaaaggagtt
    30301 tacggagcgg aggaaaagct tgtcaagcca gtcacaaggc ataccgaggt aaaaaacccg
    30361 atgtttacgg ctaatatcag cgttgtttat tttacgtcca acgtgcgaag caaattaacg
    30421 ttatgaatag ctgtaagaaa aaatccctca tcgtcatcac tgttttaaaa tgactcacta
    30481 ttttccacag caaatataaa gtgtcagttt gcgacgcgag cggcatttta ggacatatct
    30541 tccccggtta acagtccttg ttacgtctgt gtggcgcttg atgccagcgg gtttgcgcaa
    30601 aagcaaagaa aagtgtttgt taaatcggca attcatcgcc gcatgtaaca aaataatgca
    30661 aaaacgcgct gtcgatatca cttaattatt gtgaatttca gctggtgagt taattaacgg
    30721 gcgggtttaa aaccatttat caataatagt caggaatagt cttatttact ttaa
//
LOCUS       DAZSNM010000021        30212 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZSNM010000021 DAZSNM010000000
VERSION     DAZSNM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30212
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.021"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            183..1088
                     /gene="yhfZ"
                     /locus_tag="ACHW42_000860"
     CDS             183..1088
                     /gene="yhfZ"
                     /locus_tag="ACHW42_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR8942796.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            1105..1467
                     /locus_tag="ACHW42_000861"
     CDS             1105..1467
                     /locus_tag="ACHW42_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR8942797.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            1551..2714
                     /locus_tag="ACHW42_000862"
     CDS             1551..2714
                     /locus_tag="ACHW42_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFR8942798.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            2715..3941
                     /locus_tag="ACHW42_000863"
     CDS             2715..3941
                     /locus_tag="ACHW42_000863"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR8942799.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            3938..4816
                     /locus_tag="ACHW42_000864"
     CDS             3938..4816
                     /locus_tag="ACHW42_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR8942800.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            4827..5180
                     /locus_tag="ACHW42_000865"
     CDS             4827..5180
                     /locus_tag="ACHW42_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR8942801.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            5192..6496
                     /locus_tag="ACHW42_000866"
     CDS             5192..6496
                     /locus_tag="ACHW42_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR8942802.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            6508..7593
                     /locus_tag="ACHW42_000867"
     CDS             6508..7593
                     /locus_tag="ACHW42_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR8942803.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(7753..8484)
                     /gene="frlR"
                     /locus_tag="ACHW42_000868"
                     /gene_synonym="yhfR"
     CDS             complement(7753..8484)
                     /gene="frlR"
                     /locus_tag="ACHW42_000868"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR8942804.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(8584..9369)
                     /gene="frlD"
                     /locus_tag="ACHW42_000869"
     CDS             complement(8584..9369)
                     /gene="frlD"
                     /locus_tag="ACHW42_000869"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR8942805.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(9366..10196)
                     /gene="frlC"
                     /locus_tag="ACHW42_000870"
     CDS             complement(9366..10196)
                     /gene="frlC"
                     /locus_tag="ACHW42_000870"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFR8942806.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(10246..11268)
                     /gene="frlB"
                     /locus_tag="ACHW42_000871"
     CDS             complement(10246..11268)
                     /gene="frlB"
                     /locus_tag="ACHW42_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR8942807.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(11289..12626)
                     /gene="frlA"
                     /locus_tag="ACHW42_000872"
                     /pseudo
     CDS             complement(11289..12626)
                     /gene="frlA"
                     /locus_tag="ACHW42_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            complement(12921..13088)
                     /locus_tag="ACHW42_000873"
     CDS             complement(12921..13088)
                     /locus_tag="ACHW42_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR8942808.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(13335..14708)
                     /gene="cysG"
                     /locus_tag="ACHW42_000874"
     CDS             complement(13335..14708)
                     /gene="cysG"
                     /locus_tag="ACHW42_000874"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR8942809.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(14727..15533)
                     /gene="nirC"
                     /locus_tag="ACHW42_000875"
     CDS             complement(14727..15533)
                     /gene="nirC"
                     /locus_tag="ACHW42_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR8942810.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            complement(15659..15985)
                     /gene="nirD"
                     /locus_tag="ACHW42_000876"
     CDS             complement(15659..15985)
                     /gene="nirD"
                     /locus_tag="ACHW42_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR8942811.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(15982..18525)
                     /gene="nirB"
                     /locus_tag="ACHW42_000877"
     CDS             complement(15982..18525)
                     /gene="nirB"
                     /locus_tag="ACHW42_000877"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR8942812.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(18787..19968)
                     /gene="tsgA"
                     /locus_tag="ACHW42_000878"
     CDS             complement(18787..19968)
                     /gene="tsgA"
                     /locus_tag="ACHW42_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR8942813.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            20239..20820
                     /gene="ppiA"
                     /locus_tag="ACHW42_000879"
                     /gene_synonym="rotA"
     CDS             20239..20820
                     /gene="ppiA"
                     /locus_tag="ACHW42_000879"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR8942814.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            20925..21092
                     /locus_tag="ACHW42_000880"
     CDS             20925..21092
                     /locus_tag="ACHW42_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR8942815.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            21082..21684
                     /locus_tag="ACHW42_000881"
     CDS             21082..21684
                     /locus_tag="ACHW42_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR8942816.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            21716..22279
                     /gene="pabA"
                     /locus_tag="ACHW42_000882"
     CDS             21716..22279
                     /gene="pabA"
                     /locus_tag="ACHW42_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR8942817.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            22365..23585
                     /gene="argD"
                     /locus_tag="ACHW42_000883"
                     /gene_synonym="dapC"
     CDS             22365..23585
                     /gene="argD"
                     /locus_tag="ACHW42_000883"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR8942818.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(23652..25742)
                     /locus_tag="ACHW42_000884"
     CDS             complement(23652..25742)
                     /locus_tag="ACHW42_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR8942819.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(25793..26425)
                     /gene="crp"
                     /locus_tag="ACHW42_000885"
     CDS             complement(25793..26425)
                     /gene="crp"
                     /locus_tag="ACHW42_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR8942820.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            26727..27131
                     /locus_tag="ACHW42_000886"
     CDS             26727..27131
                     /locus_tag="ACHW42_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR8942821.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            complement(27186..28055)
                     /locus_tag="ACHW42_000887"
     CDS             complement(27186..28055)
                     /locus_tag="ACHW42_000887"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR8942822.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(28109..28327)
                     /locus_tag="ACHW42_000888"
     CDS             complement(28109..28327)
                     /locus_tag="ACHW42_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR8942823.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            complement(28321..29343)
                     /locus_tag="ACHW42_000889"
     CDS             complement(28321..29343)
                     /locus_tag="ACHW42_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR8942824.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            complement(29343..>30212)
                     /locus_tag="ACHW42_000890"
     CDS             complement(29343..>30212)
                     /locus_tag="ACHW42_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR8942825.1"
                     /translation="AGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
BASE COUNT         7092 a         7712 c         8155 g         7253 t
ORIGIN      
        1 tgctcgcatt gtcgaataaa tcacttcgtc atcactctga aaccggggaa acccggtttt
       61 tttatcctct tttcgcaaca acccgaaaaa atgtgaagcg cctcgccgtt tccacatctc
      121 gtcgtcgcca cttatcttca ttttaatgaa gatataaata ttcattttat tgaaaatata
      181 ttatgcgtcg aacgtttatc aaaaaagaag gcgtcgtcat cacgacactg gcccgttatt
      241 tgttgggtga aaaatgcggt aatcgactga aaaccataga tgagctggca actgaatgcc
      301 gttcatcggt tggcctgacg caggccgcgt tgaaaacgct ggaatcaagc ggagcgatac
      361 ggattgaacg ccgtgggcgc aatggcagtt atcttgtcga gatggataac aaagcattgc
      421 tgagtcatgt ggatatcaac aacgtggtat gtgcgatgcc tttgccctat acccgcttgt
      481 acgaaggttt agcgagcgga ttgaaagccc agtttgatgg cattcctttt tactatgcgc
      541 atatgcgcgg cgcggatatt cgcgtggagt gtttacttaa cggtgtctat gacatggcag
      601 tagtttcccg cctggcggcg gaaagttatc tcagccaaaa caatttgtgc atcgccctcg
      661 agcttggccc gcatacgtat gttggtgaac atcaactgat atgccgcaaa ggtgagtctg
      721 gaaatgttaa gcgagtaggt ctcgatagtc gttcggcgga tcagaaaatc atgaccgatg
      781 tcttttttgg cgatagtgat gtggaacgag tcgatctctc ttatcacgag agtttacaac
      841 gcattgttaa aggcgatgtc gatgcggtga tctggaacgt ggtggcggaa aacgaactga
      901 ccatgctggg attagaggcg acgccgctaa cagacgatcc gcgattttta caggccaccg
      961 aagccgtggt cctgacgcga gtcgatgatt acccaatgca acaattgctg cgtgctgttg
     1021 tagataaaca cgccctgctt gcccatcaac aacgggtggt gagtggggaa caggaaccca
     1081 gttattaaac gaaaggcatc tgatatggaa accagactca acctgctttg cgaggcaggc
     1141 gttattgata aggacgtctg caaaggcatg atgcaggtcg tcaacgtact ggaaaaagag
     1201 tgccatctgc cggtgcgcag tgagcaagga acgatggcga tgacacatat ggcgagtgca
     1261 ctgatgcgca gtcgccgtgg tgaagaaata gagccgctgg ataacgagtt gctggcggaa
     1321 ctggcgcaat ccagccactg gcaagccgtt gtgcaattgc atcaggagtt gttgaaggaa
     1381 ttcgctctgg aagttaaccc gtgtgaagaa ggctattttc tggccaatct ttatggatta
     1441 tggatggctg ctaacgaaga ggtttgaagc tcccgacaaa tccacgtcgc acaacctaaa
     1501 tattcaaaaa aatgaatatt taattcaagg aacagaaacg aggcaacaag atgtttgtag
     1561 aagcattgaa acgccagaac ccggcgctga tttccgccgc actaagtttg tggcagcagg
     1621 gcaaaatcgc cccggacagc tgggtgatcg acgtggatca gatactggaa aacggtaagc
     1681 ggctcattga gacggcgcgg ctttacggca ttgaactgta tctgatgacc aagcaatttg
     1741 gtcgtaatcc gtggctggcg gaaaaattgc tggcattagg ctacagcggc attgtggcgg
     1801 tggattacaa agaggcgcga gtcatgcgcc gcgctggttt gccagtggcg catcaggggc
     1861 atctggtaca aatcccttgt catcaggttg ctgacgccgt tgaacagggc accgacatca
     1921 tcaccgtgtt tactctcgac aaagcgcggg aagtttctgc ggcagcggtt aaggccggac
     1981 gggtccagcc tgtgctgctc aaagtttata gcgacgatga ttttctttat ccggggcagg
     2041 agagcggttt tgtacagcat tcgctgcacg aggtgattgc tgaaattaag aaactgccgg
     2101 ggctgcattt agccggacta acccatttcc cttgcctgct ttgggatgag gctgctggga
     2161 aagttttgcc aacaccgaat cttcacacgc tggtacaagc acgggatcaa ctggcgaaat
     2221 ctggtattgc gattgagcaa ctgaacgtac cttcagcgac cagctgcact tcgctgccat
     2281 tgctggcgga atacggtgcg actcatgccg aacccggtca tgcgctgacc ggtactattc
     2341 cggcaaacca gcagggcgat cagcctgaac gtatcgcgat gctctggtta agtgaaatct
     2401 cccatcattt ccgtggcgac agctactgct acggcggcgg ttactaccgt cgtggtcatg
     2461 cgcaacatgc gctggtgttt acgccagaaa atcaaaagat tactgaaacc aatctcaaaa
     2521 ctgtggatga cagcagtatc gactacaccc tgccgctggc aggcgagtat ccggtgagca
     2581 gtgcagtggt gctctgtttt cgcacgcaga tttttgtcac ccgtagcgat gtggtgctgg
     2641 tgtccggtat tcagcgcggc gaaccagaaa tagttggtcg ttatgacagc ctcggcaacc
     2701 ctctggaggc ataaatggcg cgatttgtgg tgttagtgat tgatagcttt ggcgtagggg
     2761 caatgaaaga tgtcacgctg gtgcgcccgc aagatgccgg ggcgaatacc tgtggtcaca
     2821 tcctgagcca gttgccgcat ttgcagctac caacgctgga gaagctgggg ctaatcaaca
     2881 cattgggtta tgcgccaggc gatatgcagc cgtcagattc cgcaacctgg ggcgtggcag
     2941 agctgcaaca tgaaggtggc gataccttta tggggcatca ggaaatttta ggcacgcgcc
     3001 cgttaccgcc gctgcggatg ccttttcgcg atgtgattga ccgtgttgag caggcattag
     3061 tttccgctgg ctggcaggtg gagcgccgtg gcgatgatct gcaatttctg tcggtcaatc
     3121 aggcggttgc gattggcgat aatctcgagg cggatttagg ccaagtctat aacattaccg
     3181 ccaatctctc tgtgatctct tttgacgacg caatcaaaat tggtcgtatc gtgcgtgacc
     3241 aggtacaggt cggtcgggtc attacatttg gcggcctgtt aaccaacagt cagcgtattc
     3301 tcgatgccgc agaaagcaaa gaagggcgct ttattggtat taatgcgccg cgttctggcg
     3361 cttatgacaa cggcttccag gtcgtgcata tgggctacgg tgttgatgaa aaagtgcagg
     3421 tgccgcaaaa actgtatgaa gcgggcgtgc caaccgtgct ggtgggtaag gtggcagata
     3481 tcgtcagcaa tccttatggt gtgagctggc aaaacctggt ggatagccag cggattatgg
     3541 atatcaccct cgacgaattt aacacccatc cgacggcgtt tatttgcatc aatatccagg
     3601 aaaccgacct cgctggtcat gcagaagacg tcgcacgtta tgccgaacgt ttgcaggtcg
     3661 ttgaccgtaa ccttgcccgg cttgttgagg cgatgcagcc agatgattgc ctggtcgtga
     3721 tggcggatca cggtaacgat ccgaccattg gtcacagcca ccatacccgt gaagtggtgc
     3781 cagtgctggt ttatcagcaa gggctggtcg ccacgcagct cggtgtgcgc accacgcttt
     3841 ctgatgtggg agctaccgtg tgtgaatttt tccgtgcgcc accgccacaa aatggtcgct
     3901 cttttctttc ctccctccgg tttgcaggag acaccctatg agttttgatc cgacgggtta
     3961 caccctggcc catgagcatc tgcatattga tctctccggc tttaaaaaca acgtggactg
     4021 ccgccttgat cagtatgcgt tcatttgcca ggagatgaac gacctgatga cccggggtgt
     4081 acgtaatgtg attgagatga ccaaccgtta catggggcgc aatgcgcaat ttatgctcga
     4141 tgtaatgcgc gaaaccggga tcaacgtggt ggcctgtact ggttattacc aggatgcgtt
     4201 tttcccggaa catgtggtga cccgcagcgt gcaggaactg gcgcaggaga tggtcgatga
     4261 aattgaacag ggtatcgatg gcacggagct gaaagccggg atcatcgcgg agatcggctc
     4321 cagcgaagga aagattacgc cgctggaaga gaaggtattt attgccgctg cgctggcgca
     4381 taaccagacc ggacgcccga tctccacgca tacgtcgttc agcaccatgg ggctggaaca
     4441 actggctctg ttgcaagccc acggagttga tctttcgcgc gtcaccgttg gtcactgcga
     4501 tctgaaagac aacctcgaca acattttgaa gatgatcgat ctcggcgcgt acgtgcagtt
     4561 cgacaccatt ggtaagaaca gttactaccc ggacgaaaag cgcattgcga tgctccacgc
     4621 gctacgtgac cgtgggttgc tgaaccgcgt catgctgtcg atggatatta cgcgccgctc
     4681 ccatttaaaa gccaatggtg gttatggcta tgactattta ttgaccacct ttattccgca
     4741 attgcgccag tcaggattca gtcaggccga tgtggatgtg atgttacgtg aaaatccctc
     4801 tcaatttttc caataaggac agactcatga aaaagattgg cgttgcaggc ttacagcgtg
     4861 agcagattaa aaaaaccatt gaagcgacgg ctcctggctg ttttgaagtt ttcattcaca
     4921 acgacatgga agcggcaatg aaggtgaaat ccgggcaact agattattac attggcgcgt
     4981 gtaataccgg tgcgggcgcg gcattgtcga ttgccatcgc ggtgattggc tataacaaaa
     5041 gttgcaccat tgccaaacca ggcattaaag cgaaagacga gcatatcgcc aaaatgatcg
     5101 ctgaagggaa agtggcgttt ggcctttccg ttgagcacgt cgaacacgcg attccgatgt
     5161 tgattaacca tctgaaataa aaggcacgac tatggatctg tatattcaga ttatcgtggt
     5221 ggcgtgcctg acgggtatga catcgcttct ggagcatcgc tcggcggctg tttttcatga
     5281 cggcatccac ccgatcctgc cgcaactgat tgaaggctat atgaaccgtc gcgaggcggg
     5341 gagtatcgct tttggtctga gcattggttt tgtggcctcg gtggggatct cttttaccct
     5401 gaaaaccggg ctgctcaacg catggttact ctttcttcct accgatatcc tcggcgtact
     5461 ggcgataaac agcctgatgg cgtttggtct tggcgctatc tggggcgtgt tgatccttac
     5521 ttgcctgttg ccagttaacc agctgctgac cgcgctgccg gtggatgtat taggtagcct
     5581 cggggaatta agctcgccgg tggtttctgc ttttgcactg ttcccgctgg tggcgatttt
     5641 ctaccagttt ggctggaagc aaagtctggt cgccgccgtg gttgttctga tgacccgtgt
     5701 ggtagtcgtg cgctatttcc cacatcttaa ccctgaatcc atcgaaatct ttattggcat
     5761 ggtgatgctg ctggggatcg cgataactca cgacctgcgt catcgtgatg aaaatgacat
     5821 cgatgccagc gggctttcgg tgtttgaaga gcgcacgtcg cggattatca aaaacttacc
     5881 ttatatcgcc atcgtgggag cattgattgc cgccgttgcc agtatgaaga tttttgctgg
     5941 tagcgaagtt tcgatcttca cactggagaa agcgtattcc gcaggcgtga cgccggaaca
     6001 atcgcaaacg ctgattaatc aggcggctct ggcagaattt atgcgcggac tggggtttgt
     6061 gccgttgatt gccaccaccg ccttagcaac gggtgtgtat gcagttgcgg gctttacctt
     6121 tgtttatgcg gtgggctatc tctcgccgaa tccgatggtt gcagcggtat taggcgcagt
     6181 ggttatttcg gcggaagtct tgctgcttcg ttcgatcggc aaatggctgg gacgctaccc
     6241 gtcggtgcgt aatgcgtcgg ataacatccg taacgccatg aatatgctga tggaagtggc
     6301 gctgctggtc ggttcgattt tcgcagcaat taagatggcg ggttataccg gattctctat
     6361 cgcggttgcc atttacttcc tcaacgaatc cctgggccgt ccggtacaga aaatggcggc
     6421 accggtcgtg gcagtaatga tcaccggtat tctgctgaat gttctttact ggcttggcct
     6481 gttcgttccg gcttaaggaa ggcacctatg aagacgtttc ctttgcaaag cctgactctt
     6541 gctgaagcgc agcaaaagca gtttgcgctg gtggacacta tttgccgcca ttttccaggc
     6601 agcgagtttc ttgctggcgg tgatttaggg ttaactccaa gtctgaatca accgcgtatt
     6661 acccaacggg tggagcaggt gctggctgat gcatttcacg cacaggccgc ggcgctggtg
     6721 cagggcgcgg ggactggcgc gattcgcgcc gggctggcgg ctttgctcaa accggggcag
     6781 cgtcttctgg tgcatgacgc gcctgtttac ccgacgacac gggttattat tgagcagatg
     6841 gggctgacgc ttattactgc tgatttcaat gacctgtcgg cactgaagca ggtcgtcgac
     6901 gagcaacaac ctgatgccgc gctggtgcag catacgcgcc agcagccgca ggacagctat
     6961 gtgctggcag atgtgctggc aacgttgcgc gcggcaggtg ttccagcgtt aaccgatgat
     7021 aactatgcgg tgatgaaggt agcgcgaatc ggctgtgaat gcggcgcgaa tgtctcgaca
     7081 ttctcctgct tcaagctgtt tgggccggag ggcgttggtg cagtggttgg cgatgccgat
     7141 gttattagtc ggattcgcgc cacgctttac tccggcggta gccagatcca gggctcacag
     7201 gcgctggaag tcttgcgtgg tctggtgttc gcgccagtga tgcatgcggt gcaggcggga
     7261 gtatccgaac ggttgctggc tttgcttaac ggcggcgcgg tggcggaagt gaaaagcgcc
     7321 gtcattgcca atgcgcagtc gaaggtgttg attgtcgagt ttcatcagct gattgccgcc
     7381 agggtgctgg aagaagcgca aaagcgtggc gcgttgcctt acccggtcgg tgcagagtcg
     7441 aaatatgaaa tcccgccgct cttctatcga ctttcaggaa cgtttcgcca ggcgaatcca
     7501 caactcgaac attgtgcgat tcgcattaac ccgaatcgca gcggtgaaga gacggtgctg
     7561 cggattttgc gtgagagtat tgcgagtatt taatgctgga tagcaccgtt cggcaatcaa
     7621 gctaaattcc gcactgaacg gtcccctcgc ccctttgggg agagggttag ggtgagggga
     7681 acgtgcggga tgtaaaattc agtgcgccag catcgccgga tgcgatgctg gcgcatctta
     7741 tccggcctac ggttaatgtc ttttattatc aatcgttaag gtgatgcgat tggcgcggca
     7801 gaagagttcg gagacgtgca ccggtttgtc attctgatca aaggcgattt tgctgatgcg
     7861 aaacaacggt tcgcccagtt cgcatttcag ccattttgcc tgcgggcggg tggcggcaaa
     7921 gatatcgatg gtctttttgt cgctgaccac tcgcgtgtca aaacgctcct gaaataactg
     7981 ataggtggag cttccttcga cgtaaatctc atcaaagtca ggataacgcg acagcgggat
     8041 ccatgaacta tcaataaaca gcggctcttt atcgagatac atcacccggc agagatggaa
     8101 cacttcgctg tttccgggga tgttcagctt ttcgcaaaac ggcgcggcgc tgacccgttc
     8161 ctgttcgatc actttctctt tcgtcgactt gccttgtgag acgccaaaat cggtaaaacc
     8221 actgacagta agcagggcgt tttcaacttt ctggctttgt acaaaggtgc cttttccctg
     8281 ccagcggatc agtacgccgt ctgccactaa gtcgctgatg gctttgcgaa tggtaatgcg
     8341 gctgacgtta tattgtgtac aaagctcgtt ttcggtaggg atctgttgcc cggcctggta
     8401 aaccccctgc gcgatatcat ccagcagccg ctggcggacg gtagcgtaga ggagttgatg
     8461 agagtagcgg tccgtagctg acataatcga ccctgaaata aagaatgaat tactcggatt
     8521 atagggtcgt tttccggcaa gtaacagcac gttggcgtga agatgctcac gccaacgtta
     8581 tacctaccag gcaccgtggt actgaatggt tttcgccgcg cacgccgttc cctgcgccat
     8641 cgcctgcggt aatgtcatcc ccgcagacca gccgcaaagg aatccggcaa tgaacgaatc
     8701 tccggcaccc atggtgtcga taaccgtcac cggttcagga gcctgacgcc agaactgcgc
     8761 gccatcccag gcaatgctgc cgttttcacc cagcgtgaca atcactgttc ctgcgccacg
     8821 ggcaacaatc gctttcatct tcagacgcag cgcttcgtct tcttgcggtg cggaggcaaa
     8881 ggcaaaatcg agatgcggca ccagtgtctg ccagagcggg ctgtcccact tgtcggagaa
     8941 gtcgaaagcg gtaagtttgc ccgcagcgtg cagctgtggg aatgcgtctt ccgcatgtcc
     9001 ccagattgcc gcgtgcacaa tgtcatactg cgccagccag gcgtaatcct cttcactcag
     9061 ggcaaagtcg gccatcacgc cttcggtgta gtcgccaaaa acgcgatcat tgtcgtgcag
     9121 ttccacctga gtttgtgcgg taacgccgtg tttcgtatgg acatggctga tatcgacgcc
     9181 catgcgggcg agatcctgct tcagctttgt gccgtagtca tcgtcaccca cccaggtaat
     9241 gcatcccggc tgtatgccgt agcgagtgca gtacaccgcc acattgaccg cattaccgcc
     9301 agaaaacgct ttattcagtt gcgggtagat atcgacgcag ttatcgccga ttgtcgccag
     9361 ggttttcatc tctcatcctc cggcagcagc gcgcgaaagc gttccagcgc ctggcgggca
     9421 tagagtctgg gctcgttcat atacatcgtc accagctcca ccgtagagta accctcatag
     9481 ccccgatcaa taatatcgcg catcagttcc cgcagcggca ttttgccttc tccaggaatg
     9541 taatgcgtgt cgctggcccc gtcgctgtcg acaatatgca gatgacgtaa tttatcgccc
     9601 agtttgtcga aataactcat caccggttcc gcctggacat acggcgcgca aatgtcgacc
     9661 atgctgaaca ggcgcggcga aggcaccagc gccagcgcat gaagcacatc attagcatta
     9721 cacacgacgt tcgattcata cggcgttaac ggttcgagga tcagatccat gccgatgttt
     9781 tccgcgtact cacacagttc gcttaagttc tctgccagcc gcccccagat aacgttaggc
     9841 ggcgtgagat agcccgcgtg ggccgcagaa atcagcgtat aacccgcgtt catctctttt
     9901 gccatatcta tcgccagctt gatcatgtcc aggctttcac gacgcatatg ttcatcgccc
     9961 agcatcatgt tatacggata gccgttggtt tctggcgtgt aaccgataat cggcatctga
    10021 tacgtctgcg ccagcgcctt gatttgtttg atgccgcccg cttttaagtc cggcgcgaac
    10081 gcgtgcgggc gaccgcccca aatttcgatg ccgtcgtaac ccagctcgct tgcatcacga
    10141 aatgcgtgtt caataggcag ccgctggtgg ccgcaggtaa acatacctgt tttcatcggg
    10201 taactccttg cgtaaatccg gcccgttgcc gggccgggag agggattaat attccaccag
    10261 accaccgtaa tagcggcgtt catccgggtt gtgatctttg taaatagaca ggtagtagca
    10321 gagccactcc attggcacga acatcaggaa cggtgccagc cacgggtgca gcccttgcga
    10381 aatttcggcg taatcgatga cgatcacgtt gtcagtacgc tgtttaacaa agttaatggc
    10441 gcgttcggtg gtgtggcgac tttcatcatt gccgagcagg aacaggaacg gaacgcccgg
    10501 ttcgacaatc tccagcgggc catggcggaa ctctccgctc tcaatcacgc agccgtgcgt
    10561 ccaggtaaat tccatcagcg ttacaatgcc ttctttgtaa cccagcggac gcagcggacc
    10621 cgcagcaacg gtataaatca tcggccactg gctggccagt tcaccaagct ggcggccttt
    10681 ttcttcccag gtgcgtacca gatgaccgag cgcattcggc aactgcttga gatcgttttt
    10741 gatcttgccg atttccgcgt tcggcgcgag gcgggtgatc atctccagca ccacgctgta
    10801 gcagagcagc aggtgaattt cccagataca gtcggcctga taatcaatgc taaattccgc
    10861 cgccgaggta atcgggctgt ccgcgcgttt ggtgaacgcc gcagtgagtg ctccgcaggc
    10921 ccggcccagc tccagcgctt tgattacctc ttccgtttta ccgtagtcag aaacgccaat
    10981 caccgcgcaa cgatcgtcga ggcgatacgg ggtgttatcg cagaactccc agccggaaat
    11041 ggcgtagacc tgaagatcgg aaaagcgatc cgccagatgt ttcgccgttt gcgccgcgtt
    11101 gagtggcgag ccgcaggcaa cgaaataaat acgatcaatg tcgcgcttca ccatctcttc
    11161 cacgatggcg tgcaccagcg gaacgtcatg gctgagaact ttttccactt cctgaaccat
    11221 attttcggtg accagaaagt ccacggtgct tttatcaata tccaacattt tattttcttc
    11281 agataacatt acgacaaagc gttgagctgg cggctacgct tcgcccagaa ggcgtaagca
    11341 ggcagaccag tagcaataac gatggcggcg cagataaggc cgggaatcgg tgcccagaca
    11401 aaggttgagg cgaccagaat gaggcttgac gcaatggcga gggtggtcat cagcccgaaa
    11461 gccggagtac gccacagcgg tttgtaatcg tcgcgtttac gacaccagat gatggagccg
    11521 aaggtgaggg tatttttgaa acacatcacc agggtgaaat aacccagcag gctggtgaga
    11581 tcggaaacga agatgaagaa gatccccagc gccccttgca ggatgatgga gacatccggc
    11641 gtgttgtatt tcggatgcgc atggccgaag catttaaaga acaggttgtc tttcgccatc
    11701 gcgtattcca gccgcggctg gtacatcacg cagctggaaa gcgaaccaag aatgacgatc
    11761 atcgccgtga tggcaacaaa gagcccagcg gtgctgccga gtgcggggat ctaggtcagg
    11821 gcgtcggaaa tcggcgtttc agaattggcg agtttgtcga agggcatcag gccggaaatc
    11881 accagcgcca gcagggtgta gagcaccaga accagcagac aggaaccaat cagcgctcgt
    11941 ggcatggttt ttccggggtt tttaatttcg ccggtcatat aacagataga ggccatgccg
    12001 gtatacgacc aactggtggc agagatcccc gccagcagcg ccataaagct gcccgttgcg
    12061 ccaatcgcag tggtggtaag ggcggcaaaa ttctccgctt taaaccagaa gatcccaagg
    12121 ccaatgacga tagtgaacgg gataattttg gcgatggtaa ttagcgtctg aaacgctgcg
    12181 ccgccttcaa cggagcgcag gtgtagcaac ataaaggcga taattaatcc ggcggcgata
    12241 aatttaccga gcaacggatc gataggcgtt aaaaagccaa gattgctgac aatcgccagc
    12301 gccataatcg acaatgacgg cgcatcgttg gcccagaagc tggcccagcc ggagaggaaa
    12361 gccagcggtc ggcttccggc atttttcaga taaacataat ctgcgccatt ttccggataa
    12421 gcggtggata gttccgcata gacgcacatt tgcgggatca caattaaccc gccaatgaca
    12481 aacgcgagca ccgtaagcca cggcgtgccc gctgcttttg ccacttcacc cacagataca
    12541 aaaataccgg agccgacggt tgtcccgacg gcgattgcaa gaacggccca aaatccgagc
    12601 ttgcgttgga gttcctggct tcccatagcg ataccttttt cttgtccaat acgttgtagg
    12661 ggagagtttg cagccattta aaaggtaaca tgacaatgca tgatgaatat aacaacatat
    12721 tatgttatgt gatgtggagg aagatcacaa aatttcgcac aggatcgcgc tgtggctaat
    12781 ggatgtagtt ataaaattga atttaaagtg aaaatatttt tacgggcggg ggcaagaagg
    12841 acatagaaac aaatacgccc tcggaaaatc cagagggcgt cgggcaatta aaccggtgtt
    12901 agccgatttc tgtcagagac ttactgtgca gtaggaccgc aaccgccaat gattttagaa
    12961 atagaaattg ccgggtgcag caggtagtcg taagagcagt tcttatcacg gttttcaata
    13021 tgaccagtac atgcagttaa cgtagccagt actgcaccta ccagtgcaac tttaataaat
    13081 ttgttcattt acattcccta tgtattatat attaattaat attcattgat tatatgaata
    13141 taagcagact gctcgttaag aacgatacag gaatatttaa ttggcgtcag tgagcgaatg
    13201 acttaatttt gtaaatcgct gtaacggtga atataaccca ttagcagata atatttatta
    13261 aggggttatg ttttatttgc tgaatatata aaaagagtca ttaagaaggc cagaattctc
    13321 tggcccgggt taaattaatg attggagaac cagttcagtt tatcgcgcag gccaacaacc
    13381 cgaccaataa taattagcga tggactgttc atttgctgag caagttcacc gagttgcgtg
    13441 agcgtaccgt caatcacgcg ctgcgtgact gccgttccgt tttcgacaat tgccaccggc
    13501 atttcgccag gcataccgtg ttcaatcagc ttttgctgaa tagtcgcggc ctgattcaac
    13561 cccatataga acaccagcgt ctgtttttct gccgccaggt tttcccagtc cagctcgcca
    13621 ccggttttta agtgtccggt aattaagcgt acgctctggg cataatcgcg atgcgtgagt
    13681 ggaatacccg aataggcaga gcaaccagaa gctgcggtaa tacccggaac caccgagaac
    13741 ggaatacccg cgttgcacag tgtttccagc tcttcgccac cacggccaaa aataaacgga
    13801 tcgccacctt tcagccgcac cacgcgtttg cctttttgcg cttcccgcag caggatctgg
    13861 ttaatctctt cctgcggtac acagtggtat cccgcgcgtt tgccgacgaa aacgcgatca
    13921 gcatcgcggc gtaccagatt cataatatcg tcagaaacca gacggtcgta gaccaccaca
    13981 tctgcctgct gaatttgttg cagtcctttc agcgtcagca gcccggcatc gcccggacct
    14041 gcaccaacca gcaccacttc accgcgatgg tcgagcggtt cgttgattaa ctgttcggtc
    14101 gtttcagtaa tggctttctg atcgttgttt gccagcgact gcgccaggcg gtcgttaacg
    14161 aacaatttct cccagaaacg gcgacgctca ctcatcgtgg cgaactgttg tttcactcgc
    14221 ccgcgtaatt gcccggcata tttcgccacc tggcccagat gcaacggcag cagtgattcg
    14281 agtttttcgc gcaacaggcg cgccagaacc ggagaggtgc cgccagagga gaccgctacc
    14341 attagcggtg agcggtcaat aatcgacggc ataataaagc tggcggcttt cggcgcatca
    14401 actacgttac agaagatgcg acgagcttca gcggcttcgc tgacgcgctg gttaagcgtg
    14461 tcatcatccg tcgctgcaat cgccagccag caggtgtcga gaaggctttc atcaaatggc
    14521 ccttcgacga gggttaacat gcctgcatct gcccatgcgg tgaactgtgg aataaacgct
    14581 aatgcattca ccgttaagcg agcgcctgcg tctaacagca accttgcttt gcgttccgcg
    14641 acatcaccac cgccgacaat cagacagtcg cgatcgcgta attggcaaaa tataggcaaa
    14701 tgatccacgt aaaaacccct tagtaattaa ccggcagctg tttcagtttg attaaatttg
    14761 tccgcaaccg gacgattcgc tttcggcgta gcataccaat aacccaatcc catgaatacg
    14821 gcacctgata aagtattacc cagcgtcacc cacagcaggt tatgaccaat acccgccagc
    14881 gtgtaggctt cgctgtggtt gccgaaccag gagagcgcga acagcgtcat gttagcgata
    14941 gagtgctcgt agccggacgc gataaatgcc agcagacacc accagatagc gataaatttc
    15001 gccgcccctt cagtgcgcag cgccatccag attgccaggc aaaccagcca gttacacaat
    15061 gcacctttga agaacagtac cattgccggt gcagtggttt tagccagcgc gacggagtga
    15121 acgatgctgg tatctaccgg cagcaggcta ccgccgcccc agctatagag catggcaacg
    15181 aagacggaac cgaccaggtt acccagccag gtttgcggca ggattgccca catttgcccg
    15241 tggctgatgc tgcccgcttt taccccaaag gtgaggaaca tggtgtgtcc ggtgaacagt
    15301 tcagaaccgg cgataatcac cagcgttaag gcgataccaa aggtcgcgcc catcaccagc
    15361 ggacgtacgg acgggtcgag caaattaccg agcgtgaaaa tcaggatgat cccaagaccc
    15421 acatatgcgc ccgccatggc ggagctgacc caaaagccga gcgggttatt tgccgacagg
    15481 cgtgcaatgc gcgcagcatt agccgcacac ttattaatag tgtctgtaaa catttgatta
    15541 tcctttaaaa aataaaaaca aaaaataaaa caaaaagttt aaaaaatgtc ctgcaatata
    15601 ccgaaggtca ttacaggacc atgcaaaaag gggaggcatt gcgcctcccg ttaaaacatt
    15661 aaccgcgcag ctgcaccacg ccgtctttca ctcgtgcttc gtaatgtttg acggaaaact
    15721 gttcgtcttc catgcacaag ccatcactta agcgaaaacg ctgttttttc agcgggctgg
    15781 cgacccacag ctcgccctgg tgttccgcaa tcagcccgcg tgatagcacg ctggactcga
    15841 agaacggatc aatgttgctg atcgcaaaca cctggtcgct gtgatacggg cggaaaatcg
    15901 ctacctgctt gtcacctaac agtgcgcaga cgccggtttc aggcaggatg tcatcgattt
    15961 tgcagatgtc tttccactgg ctcatgcgtt gtcctccacc agagttaccg ggatacgttc
    16021 atacggcgtt gccggacggt gctgttcgcg ctctggcacc atctgcacgt tcgggtcacg
    16081 cttgtcgctg ttgatgaagt gtttgaagcg agtctgcgcg gacggtgtat tgaccgtttc
    16141 agtccactca cacactaccg cttcacgcag gcgcgccatc tcttcttcca gatgagcgtt
    16201 cagccccagt ttgtcgtcaa tgatcaccgc tttcaggtaa tcgatgccgc cttcgaggtt
    16261 ttctaaccac ggcgcggtac gcgtcagttt gtcggcggta cggatgtaga acatcatgaa
    16321 gcggtcgaga tatttgatca gcgtttcgcg atcgatatcc gctgccagca gatccgcatg
    16381 acgcggtttc atgccgccgt taccgcaaac atacaggttc cagccttttt cagtggcgat
    16441 aatacccacg tctttaccct gagcttctga acattcacgg gtacagccgg agacaccgaa
    16501 cttcattttg tgcggcgtac ggatgccttt gtagcggttt tccagttcca cgccgaggcc
    16561 gacgctatcg ccaacaccgt agcggcacca ggtgctaccc acgcaggttt tcgccatacg
    16621 cagtgctttc gcataggcat gaccggtttc gaagccagct tcaatcagct gacgccagat
    16681 ttccggcaga tcgtctttct gtgcgccaaa catcgccagg cgctgggagc cggtgatttt
    16741 ggtgtagaga ttaaattcac gcgcgatacg acccactgcc atcagccctt ccggggtgat
    16801 ttcaccgccc ggagaacgcg ggatcaccga gtaggtgccg tctttctgga tgttagcgag
    16861 gaagttgtcg ttagaatcct gcagcggagt atgttccggc ttcagaatgt attcgttcca
    16921 gcaggaggcc agcagcgaac cgacggttgg tttacaaact tcacaaccgt agcctttgcc
    16981 gtgtttcgcc agcagttctt cgaaggtttt aatgccttca acgcggatca aatggaacag
    17041 ttcctgacgc gaataagcaa agtgttcgca caggttgttg ttaacttcga tgccctgttt
    17101 cgccagttcc gcgttcagta cctgagtgac cagcgggata cagccaccgc agccagtacc
    17161 cgctttggtt tcagctttca gcgccgcaac tgtgtggcag cctttgttga tggcagcaat
    17221 cagatcacct ttagtgacgt cgaagcagga gcagatttgc gcgctgtccg gcagtttatc
    17281 aacaccgata gacggcttac cgctacccga gtgcgccggc agaatcagag aatccgggtt
    17341 ttccggcagc tcgatagcgt tcagcaccag ttgcagcagg ttgccgtagt cgctggtatc
    17401 gcccaccagt accgcaccga gcagggtttt gttgtcttcg ctgacaatca ggcgtttgta
    17461 gatctcttta ctttcgtcga ggtaaacata gctacgtgcg ccaggcgtgc gaccgtgcgc
    17521 atcaccaata ccacctacgt ctacgcccag cagtttcagc ttggcgctaa ggtcagcacc
    17581 ttcaaaggcg ttttcgctac ccagaatatg gtcaacggcg acctgcgcca ttttgtagcc
    17641 tggtgctacc agaccaaata cacggttgtt ccagcttgcg cattcaccga tggcgtagat
    17701 atccggatcg gaagtctggc aggaatcatt aatgacaatc cccccacgcg gagcaacgtc
    17761 cagaccacac tgggttgcca gcttatcgcg cggacggata ccggtagaga agacgataaa
    17821 gtcgacttcc agttcgctgc cgtcggcaaa acgcatggtt ttacgcgctt cgacaccttc
    17881 ctgcacaatc tcaagggtgt ttttgctggt gtgaacgcgc acgcccatac tttcgatttt
    17941 gcgacgcagc tgctcgccac ccatctgatc aagctgttcc gccatcagca taggggcaaa
    18001 ttcgataacg tgggtttcaa tacctaagtt tttcagcgcg ccagcggctt ccagacctaa
    18061 caggccgcca ccaacaacgg caccgcgttt gctgcgacgg gcgcaggatt caatggcgtt
    18121 gaggtcttca atggtgcgat agacaaagca gtcctgagta tcagaacctt tgattggtgg
    18181 gatccacggg taggaaccgg ttgccatgat cagcttgtca taaaaaacgg tacgtccggc
    18241 gctggagtga atcaccttct cctgacggtt gatggtgata gcgcgttcgc cgaccagaac
    18301 tttgatgccg tgtttctcgt agaagccttc gcgcaccagc gacagctctt cggcggtgtg
    18361 gtgagagaag taagatgaga ggtgtacgcg gtcataagcg atgcgcggtt cttcacagaa
    18421 aacggtaata tcaaagttgg ccgcatcaga tttatcaaga agatcttcga taaagcgatg
    18481 gccgaccata ccgttaccga taattgcgag tctgactttg ctcatttttg cctcgatttc
    18541 ttttctatta ccgcctacct taacgattca gcaaccccgc ttattgatgt aaatcaaatt
    18601 cacctttata tactccttaa ggggtatatt gctgatttgt ataaattttc ttaagtcacg
    18661 gaattgtctg acatttcatg tttgtgcaaa aaatggacaa aatgaacggg tttatagaaa
    18721 tagatgcacg tagcggggga agagaggaaa aggccggacg acgatagtgc cgtccggcat
    18781 tagcaattaa tgagaggtca gggtgttatg ctgacggtga cggctgacga aacctaacag
    18841 gaagcacatc acaaagacga cagcgtacag accgtttgct gtaagcagtg ccgcctgcgg
    18901 accgctatgt tcaacaatcg tgccggtaac cacaaaggtc aacatagtac cgatggttcc
    18961 gcaggtcagg acaaagttaa ccagttttgg cgacggtact ttggtctgct gtgaacccag
    19021 ggtgatgatg gtggtataga tcgcgctgga gaagaagccc agagcgagaa ttgaccacgc
    19081 catatgtact ggtgttccgg tgttaaagac gtacatcaga atcgcagcca gaccagccag
    19141 tacggtcaga atgcgttgca aatcaaagaa gcgaagaata aagctgaacg cccacatgcc
    19201 gaccatgtat gacatccaga agttactcac cagcgtgccc gcgtcgttca ggctcatgcc
    19261 caggcctttg gcatactcag gcacccagga gataaaacct aactgaccga ggatgtagca
    19321 cagcgccgca acagagagaa acagtacgcc gatcccccac ttttctttct ctaccggagc
    19381 atccgttttt ggtgcacgtt tgcccagtgc cgggaactca cagccgaagg tcagaataaa
    19441 aatagcgaca tacaccagcc cgatgcaggc ataaacccag taccactcaa tgctgcgcgc
    19501 cagtagaaac gcggcgatca ttgggaaaat catcccagcc atactgaaga aggagtcagt
    19561 aaataacagg cgggaaccgc gctgacgccc ttcatacatt tgtgttatca ggaatgtacc
    19621 aatcgacata gtaatgccgc tgaccacccc gagaatgaac atcgccgtcg agaacagcgc
    19681 caggctgtgg ctgaacatca aaccggcaac cgccagcacc atcaggagaa agccaaaacg
    19741 taactgcgtt ttcaacggga cgatttccat cagccaggcg ttgaggaaga tagagattaa
    19801 aatgccggcg ttgaggaagg tgaaggtgtt actcatactg gaaacaggca gattgaaata
    19861 atcggcgata tttcccatca ccatcccggt gacaataacc aacgcaccag tcagtgcgta
    19921 ggagagaaag ctaatccatg tgagcttgat gcgattgctg ttagtcatga ttggcctgcg
    19981 ttcaaaaata aaatggcata gcgggatatg ccgcgagcgg gcgattttag gtgattttat
    20041 gatctgttta aatgttttat tgcaatcggt tgctaaattg cattttaaga ggtgattttg
    20101 atcacggaat aaaaagtgat cgtcaggtta catatatttc agatacgtaa aattaggtaa
    20161 agggatggcc ttgttcttga aggctattta gaatctcttc acttgctttt tttctgctct
    20221 gtttgttaag gaaatctcat gttcaaatcg accctggcgg cgatggctgc tgttttcgct
    20281 ctttctgctc tttctgctct ttctcccgca gcaatggcag cgaaagggga cccgcacgta
    20341 ttgttgacaa cctcagctgg taacatcgaa ctggagctgg ataaacaaaa agcgccagtg
    20401 tctgtgcaaa actttgtcga ttatgtgaac agcggttttt ataacaacac tacctttcac
    20461 cgcgtcattc ctggctttat gattcagggc ggcggtttca ccgagcagat gcagcagaaa
    20521 aaaccaaacc cgccaatcaa aaatgaagcc gataacggcc tgcgcaacac gcgtggcacc
    20581 atcgcgatgg cgcgtaccgc tgacaaagac agcgccacca gccagttctt tatcaacgtt
    20641 gccgataacg ccttccttga ccatggtcag cgtgatttcg gttacgcggt atttggtaaa
    20701 gtggtgaaag gcatggacgt tgccgataag atttcccagg tgccgactca tgatgttggt
    20761 ccgtaccaga atgtgccgtc aaaaccggta gttatccttt ccgctaaagt cctgccgtaa
    20821 tgatttctcg cgcgggcaat cttgcccgcg cttctgctct cccggcgtaa cccggatttg
    20881 ccgcttatac ttgtggcaaa tggacacgtt cagggaggca tcaagtgaag aaactcaccg
    20941 ataagcaaaa gtcccgtctc tgggagcttc agcgtaatcg taatttccag gccagtcgcc
    21001 gtcttgaagg cgtcgagatg cctttagtca ctcttactgc cgcagaggct ttagcgcgcc
    21061 ttgaagagct gaggaggcac tatgagcgat aaattcggcg aagggcgcga tctgtatctt
    21121 tatcctggcc ttgatatcat gcgtaaccgg ctgaacatcc gccagcagca gcggctggaa
    21181 caggccgctt acgaaatgac ggcgctgcgt gctgcgacca ttgagcttgg tccgctggtg
    21241 cgcggtttac cgcatttgcg cactatccat cgccagctgt accaggatat tttcgactgg
    21301 gcagggcaac tgcgtgaagt tgatatttat cagggcgata cgccgttctg ccactttgct
    21361 tatatcgaaa aagagggcaa tgccctgatg caggatctgg aggaagaagg ttatctggtt
    21421 ggcctggaga aagcgaagtt cgtcgagcgg ctggcgcatt actattgtga aatcaacgtg
    21481 ctgcatccct tccgggtggg aagtggtctg gcacagcgga tcttcttcga gcaactggcg
    21541 attcatgccg gatatcaact gagctggcag ggtatcgaaa aagaggcctg gaatcaggca
    21601 aatcagagtg gggcaatggg ggatctcacc gcactgcaga tgatatttag caaagtggta
    21661 agcgaagccg gggaatctga gtaaaatagc gcggttcttt cgtaccggag ccgccatgat
    21721 cctgcttata gataactacg attcttttac ctggaacctc taccagtact tttgtgaact
    21781 gggggcggat gtgctggtta agcgcaacga tgcgttgacg ctggcggata tcgacgccct
    21841 taaaccacaa aaaattgtca tctcacctgg cccctgtacg ccagatgaag ccgggatctc
    21901 ccttgacgtt attcgccact atgccgggcg cttgccgatt cttggcgtct gcctcggtca
    21961 tcaggcaatg gcgcaggcat ttggcggcaa agttgtgcgc gccgcaaagg tcatgcacgg
    22021 caaaacctcg ccgattacac atagcggtga gggcgtattt cgggggctgg caaatccact
    22081 taccgtgaca cgctaccatt cgctggtggt ggaacctgac tcattaccag cgtgctttga
    22141 cgtgacggcc tggagcgaaa cccgcgagat tatggggatt cgccatcgcc agtgggatct
    22201 ggaaggtgtg cagttccatc cagaaagtat tcttagcgaa caaggacatc aactgctggc
    22261 taatttcctg catctctgat ttccgattgc cattcagtga ttttttatgc atattttgtg
    22321 attataattt cacatttatt tatgcgtaac agggtgatca tgagatggca attgaacaaa
    22381 cagcaattac acgcgcgact ttcgatgaag tgatcctgcc gatttatgct ccggcagagt
    22441 ttattccggt aaaaggtcag ggcagccgaa tctgggatca gcaaggcaag gagtatgtcg
    22501 atttcgcggg tggcattgca gttacggcgt tgggccattg ccatcctgcg ctggtgaacg
    22561 cgttaaaaac ccagggcgaa actctgtggc atatcagtaa cgttttcacc aatgaaccgg
    22621 cgctgcgtct tgggcgcaaa ctgattgagg cgacgtttgc cgaacgcgtg gtgtttatga
    22681 actccggcac ggaagctaac gaaaccgcct ttaaactggc acgccattac gcctgtgtgc
    22741 gtcatagccc gttcaaaacc aaaattattg ccttccataa cgcttttcat ggtcgctcgc
    22801 tgtttaccgt ttcggtgggt gggcagccaa aatattccga cggctttggg ccgaaaccgg
    22861 cagacatcat ccacgttccc tttaacgatc tccatgcagt gaaagcggtg atggatgatc
    22921 acacctgtgc ggtggtggtt gagccgatcc agggcgaggg cggtgtgacg gcagcgacgc
    22981 cagagttttt gcagggcttg cgcgagctgt gcgatcaaca tcaggcatta ttggtgtttg
    23041 atgaagtgca gtgcgggatg gggcggactg gcgatttgtt tgcttacagg tactacggtg
    23101 tgacgccgga tattctgact tctgcgaaag cgttaggcgg cggcttcccg attagcgcca
    23161 tgctgaccac ggcggaaatt gcttctgcgt ttcatcctgg ttctcacggt tccacctacg
    23221 gcggtaatcc tctggcctgt gcagtagcgg gcgcggcgtt tgatatcatc aatacccctg
    23281 aagtgctgga aggcattcag gcgaaacgcc agcgttttgt tgaccatttg cagaagatcg
    23341 atcagcagta cgatgtgttt agcgatattc gcggtatggg gctgttaatt ggcgcggagc
    23401 tgaaaccaca ctacaaaggt cgggcgcgtg atttcctgta tgcgggtgca gaggttggcg
    23461 taatggtgct gaatgccgga ccggatgtga tgcgtttagc accgtcgctg gtggtggaag
    23521 atgcggatat cgatgaaggg atgcaacgtt tcgcccacgc ggtggcgaag gtggttgggg
    23581 cgtaaataag acgtatcagg caaaatatgt cggatgcggc gtgaatgcct tatccgatgg
    23641 tttagcctgt attacgcctt cgaatcccgc aacttgcgac tcagccaaat cccgtgatgc
    23701 ggtcgctgac gccatgccat cgacgaaatg gtgtgcatgg tgttcagatg gccaataacc
    23761 cgctgtaaat gctgctccag cgtacctgcc gcgccttcgt gcggctgcat ctccggcgca
    23821 tccatgatat tggcatcgcc agaactaccc ggttcgtcat actccagtcg ctgctgacaa
    23881 cgctgaatgg cgatttcaca agactgtaaa tactcttgtg ccagttcagg tggcaatgcc
    23941 cggtgttccc gcgccagcgt ggtcatggca ttaatatgct caacaataaa ctggctgtgc
    24001 gttacccaca gtttcatatc tgccagataa tggctgttga atgccggttc ctgcatcgcc
    24061 tgattcaatg agttatacag agtgttatgc gcctgattta cccgcattct ctgccaggcc
    24121 agtggcgttg gttgcgggtc ctcgctcaga atcaagcgaa tcgcttcctg ataggcttct
    24181 aaagcatcat gggcgttttt acgcaataac ccgctctgcc actgcggcca cagccagacg
    24241 gtaccaccga aagcaatcaa acaaccaata atggtatcga taagacgtgg aaggatgtac
    24301 tgctcaccgt tcagccacaa cagttgcagg gtatacactg cggtaatagt aaaaccgacc
    24361 gttgcccagc cgtagttttt gcgcaatatc aggtagctgg cgagggtggt aatcagcatc
    24421 aacgtcaggg tgtaactttc gggaatttta aagtgcagcg ctacgcccgc aatgattaac
    24481 ccgaccacgg ttcccacgga gcgattcaca atcctcagac gggttgcgcc atagccattt
    24541 tgtgtcacca gcaataccgt catcaggatc cagtacgact tcggcagatg cagcacggtg
    24601 cccatcaggc tggcaacgct taacatcaca ctgagtcgtc cggcattgcg tagcgccgga
    24661 gactttagtg acagataact tttcagtgcc ggaagcaacg gcattcgccg ctgtttatcg
    24721 gccagtaagt cacgggcata gagcggtttt tgggtgcgca gcacgcgggc gatgcggctg
    24781 aaatgccagt agcagaattg cccaaccgga ttatccggat gctggcgggc gattttttcc
    24841 agtgcgccaa tttgcttttc catcgtaaaa cgcgttggca ggcgatggta aagaatgtca
    24901 tcagccagca cgcgcaggcg agcggcgacg gtttgcgcat tccagcggat aacttcttcc
    24961 gcatggctac gctcgaccag tttttgcacc tcttccggct gatgcaaact gaccgaaata
    25021 tgttcctgca aatccagcgc ctcctggaaa atacgcagca tccgcttgta atcggtattg
    25081 ttttgcgcgg aaagcatatg catttgctga tagcactgag taattaaatc gaccgctttt
    25141 tgctggcgca ccagcagcgg tggcagcgct ttttcaggat cggtgtgctg ggtaagcagg
    25201 ctgtatttgg cttcacaata atctgccagt tcacggtaca gcagacttag tgactcgcgc
    25261 agcggttgtt cgcgccagat ccagaaccaa aaccagttaa acaatccgta ccagagagtg
    25321 cccaacgcat agatgagcaa tggttcccag accggcatgt atcccgccag actgagggta
    25381 aaaatggcgg cgagcagcga tgcaggaagc aattttgcgt gtaatggccc cagctcagca
    25441 gtgacgccaa gtaccagcgt taatccggtc agcaaaaagg gcaggggaac atcttttacc
    25501 agtagcagct gtgtcagtaa gctacaggtg gcaaacagcg acgcaccaat gattaagtgt
    25561 ttgaaaaaac gtttatgagg cgtatcaagg cccgcaatat tgcaacaggc aggaacgagg
    25621 gagaagagca gaccgaatcg taattcgcca agcattaacc caacggccac gggcaaacat
    25681 agcaccagcg tttgtcgaag tgcatagttg atatcggggt gataaatcag tctgcgccac
    25741 atcgggggaa acaaaatggc gcgctaccag gtaacgcgcc actccgacgg gattaacgag
    25801 tgccgtaaac gacgatggtt ttaccgtgtg cggagatcag gttctgatct tccagcatct
    25861 tcagaatgcg tcccacggtt tcacgagaac agccgacaat ctgaccgatt tcctgacggg
    25921 taattttgat ttgcataccg tccgggtgag tcatagcatc tggttgtttt gccaggttca
    25981 gcagagtctg tgcaatgcgg cccgtcacgt cgaggaacgc caggttgccc actttctctg
    26041 aagtgacttg cagacgacgc gccatctgtg cagacagacg catcagaatg tccgggttta
    26101 cctgaatcaa ttggcgaaat tttttgtacg aaatttcagc cacttcacag gcggttttcg
    26161 cacgtaccca tgcgctacgt tcctggccct cttcaaacag gcccagttcg ccaataaaat
    26221 caccctgatt cagataggag aggatcattt ctttaccctc ttcgtctttg atcagcactg
    26281 ccacagagcc tttaacgatg tagtacagcg tttccgcttt ttcaccctgg tgaataagcg
    26341 tgctcttgga tgggtactta tgaatgtggc aatgagacaa gaaccattcg agagtcgggt
    26401 ctgtttgcgg tttgccaagc accatgcgcg gttatcctct gttataagct ttctccagag
    26461 ccagataacg ccgctgtctc tggattgccg aaatatgctt cccgctacct gggaaggggc
    26521 tatcaactgt actgcacggt aatgtgacgt cctttgcata catgcagtac atcaatgtat
    26581 tactgtagca tcctgactgt tttagcatag ctttcgcttt gtgtctcctg gtgtctcgct
    26641 tcagcatgac ccaggtcgcc ttccgttgcg cgatttggtt agtacgcgta ctctgtcagg
    26701 aaaattgacg cagtggagta gcaaaaatgc aagcgcgagt gaagtgggtc gaagggttaa
    26761 cttttctggg cgaatccgcc tctggtcacc agattttaat ggacggcaac tcaggcgata
    26821 aagcaccaag tccgatggaa atggtgttga tggcggcggg tggttgcagt gccatcgatg
    26881 tggtttcgat cctgcaaaaa gggcgtcagg atgtggtcga ttgtgaagta aaattgacct
    26941 ctgaacgccg cgaagaggca ccacgcctgt ttacgcacat taatctgcat tttatcgtca
    27001 ccggtcgcga cctgaaagac gcagcggttg cgcgtgcggt tgatctctct gccgagaaat
    27061 attgttcagt ggcgttgatg ctggaaaaag cggtgaatat tactcactcg tatgaagtgg
    27121 ttgccgcgtg acttaactat cccgttgtga agccggatag tattttatcc ggcataatag
    27181 caaagttact cgattttctt tccttccatc aatcgttgca ccagcggcag cataattaat
    27241 tccattgcca gccccatttt gccgccaggt accactaacg tattgatgtg ggaaatgaat
    27301 gagccttgca acatcgccag cagccaaggg aaatcgatcc cttccagatt acgaaaatgg
    27361 atcaccacaa agctttcatc cagcgacggg ataccttttg ccgcgaacgg gtttgaagtg
    27421 tcgacggtgg gaacgcgctg gaagttaaga tgggtgcggg aaaactgcgg tgtgatgtag
    27481 ttgatatagt cttccattga acgcactacc gagtccatca ctgcttctcg tgagtgcccg
    27541 cgctcgctgg tgtcgcggat cagtttttga atccactcaa ggttaacgat aggcactacg
    27601 ccgaccagta agtccacatg ctgcgcaacg ttatgctgtg gcgtgactac gccgccgtgt
    27661 aaaccttcat aaaacagtac atcagtgggt tctggtaaag gttgccaggg ggtgaatgtt
    27721 cccggtacct gattccaggg tacggcttcg tcgtaggtat gcagatattt gcgagatttc
    27781 cctttgccgc tctgaccgta ttcaatgaag gtttgttcca gcaggccgaa gtcgttcgct
    27841 tcggggccga agtagctgat atgccgcccg gcgtcgcgcg ctttgcggat cgccatgtcc
    27901 atttccgggc gggtgtaacg gtgaaaactg tcgccttcca cctcagctgc atgcagattt
    27961 aactgcgcga atattttacg aaacgcgagg ctggtggtgg tggtccccgc gccgctggat
    28021 cctgttaccg caatgaccgg atgtttggca gacatagtaa ctccatgact tagctatcac
    28081 tattatctcg tccgccgacg gttgcaggtt attcgcgaaa ctgactgcgc ggcataatat
    28141 tgaccgtttc gtgcagctcc gaccatacca gcaccgcttc tccgcactgt agctggcgtt
    28201 tgacgtcggc gactttctgt tcaagtgtac gctcatgttc accataatcg gtgccttcac
    28261 gtaacacaaa gctttcaatt aaattttcca gcgtttcggg ggagaggtct tgccacggaa
    28321 tcaacatgat ttcgcctcca gatacgttgt taaccagtca ggaatgcgtg actccagcca
    28381 catttgcgga tgaagtaatg taccgccaat aaagccaaca tgaccgccat gttcagtcag
    28441 ttgatactcc acctgcgagg ggagactttc cggttttggg atcacctgat gatccataaa
    28501 cggatcgtct ttggcgtgaa taatcagcgt cggtttggcg atccggttca gcattggcat
    28561 ggcgctacac tgacgataat agtcgatagc gtcggcgtag ccgtgaattc tggcggtgat
    28621 tagatcgtca aattcacgga tgcgacgtac cgattttaac tgcgcgagat taatcggcag
    28681 cgttccgggg taggctgcca gcttgcgcgc ggcattggct tttaacaggt tcagcaagta
    28741 acgctgataa acgcgggaaa agcccttttc catatgatag ctacaggctt ccagcataaa
    28801 cggcgcagag acaatcaccg ccgcatcaac cgggagatca ttgccttcct ttgccagcaa
    28861 acaggccagc atattaccgc cgagcgaata gccgacggca gccgttggcg catgaccaaa
    28921 ttcgcgttgc agccagcgta aaaaccaact ggcgtcttcg gtttcgcccg aatggtaaat
    28981 gcggtgcata cggtttggtt caccgctgca tccgcgaaaa tgcatcacca cgcccagcca
    29041 gccgcgcttt tgcgccgcct caaccagacc gtgggcgtaa gggctattga gactgccttc
    29101 cagcccgtga aacaccacta aacgcggttt atgtttcgcc tgtgcggggt cttcactcca
    29161 cgcgagatcg acaaaatcgc cgtcgggcaa ctccagccgc tgccagtacg gggtgaattt
    29221 cacctgacga cgaaacaaac gcggcagcat agtttgtaga tgacaattgc taaagccgcg
    29281 cataggggtg aattcagcac tgctgctaaa ttcattggca tcggtcgtcg ttatctgcgc
    29341 catcagttgc tttggccttc cagcagcatc tgctcaagct gctcctgggc ttccagccac
    29401 gccatttcgc actcttccag gccggatttg gcgctggctt gctgttgcag gcaggcggtc
    29461 aactccgctt tacggctctg gtcatacagt tcgctgtcgc cgagtttctc ttccgcctgc
    29521 gccagttgcg cgttcagctt ctccatctct ttttccagac gggcaatctc tttacgcagc
    29581 ggctgggttt gcgcacgcag ctccgcttcc cgacgcttct gatctttacg tgcctgggcg
    29641 ctgttcgcgt tctcttttgg cgcttcgtcg gtctggtttt cctgcttttg tacgtcgctc
    29701 aaccactgtt gataatcttc cagatcgccg tcgaacggtt cgactttacg atcgtgaacc
    29761 aggtagagat cgtcagtggt ggaacgcagc aaatgacggt cgtgcgaaac gacaaccagc
    29821 gcgccttcaa actcgattaa tgcttcggtg agtgcctgac gcatgtcgag gtcaaggtgg
    29881 ttagtcggtt cgtcgagcag cagcagattc ggacgctgcc agacaattaa tgccagcacc
    29941 aggcgggctt tttccccacc ggagaagcgg cgcgtttctt cggttacttt atcgcccagg
    30001 aaaccaaagc cgccgaggta gtcacgcagt ttttgttcca gctcctgcgg cgctaaacgt
    30061 gccagatgtt gaataggtga ttcgtcggcg cgcaggtatt caagttgatg ctgggcgaag
    30121 tagccgagct tgatcccttt cgccagacca atttcaccgc tgactggcgc aagttcaccg
    30181 gctaacagtt tgattaatgt cgatttacct gc
//
LOCUS       DAZSNM010000022        29798 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZSNM010000022 DAZSNM010000000
VERSION     DAZSNM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29798
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.022"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..851
                     /locus_tag="ACHW42_000891"
     CDS             <1..851
                     /locus_tag="ACHW42_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR8942826.1"
                     /translation="RHIIIGNEGALCYITEVTVKIFKYTPENNLFYGYILEDMKTGFN
                     ILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEI
                     VARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEI
                     YESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNP
                     LNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGT
                     IYPIEK"
     gene            873..2282
                     /locus_tag="ACHW42_000892"
     CDS             873..2282
                     /locus_tag="ACHW42_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8942827.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2260..3039
                     /locus_tag="ACHW42_000893"
     CDS             2260..3039
                     /locus_tag="ACHW42_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR8942828.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3036..3896
                     /locus_tag="ACHW42_000894"
     CDS             3036..3896
                     /locus_tag="ACHW42_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR8942829.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4044..4619)
                     /locus_tag="ACHW42_000895"
                     /pseudo
     CDS             complement(4044..4619)
                     /locus_tag="ACHW42_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4636..4896)
                     /locus_tag="ACHW42_000896"
     CDS             complement(4636..4896)
                     /locus_tag="ACHW42_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR8942830.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4887..6158)
                     /locus_tag="ACHW42_000897"
     CDS             complement(4887..6158)
                     /locus_tag="ACHW42_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR8942831.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACHW42_000898"
     CDS             complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACHW42_000898"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR8942832.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6917..8716
                     /gene="cysJ"
                     /locus_tag="ACHW42_000899"
     CDS             6917..8716
                     /gene="cysJ"
                     /locus_tag="ACHW42_000899"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR8942833.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8716..10428
                     /gene="cysI"
                     /locus_tag="ACHW42_000900"
     CDS             8716..10428
                     /gene="cysI"
                     /locus_tag="ACHW42_000900"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR8942834.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10502..11236
                     /gene="cysH"
                     /locus_tag="ACHW42_000901"
     CDS             10502..11236
                     /gene="cysH"
                     /locus_tag="ACHW42_000901"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR8942835.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11500..11652
                     /locus_tag="ACHW42_000902"
     CDS             11500..11652
                     /locus_tag="ACHW42_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR8942836.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11846..12937
                     /locus_tag="ACHW42_000903"
                     /pseudo
     CDS             11846..12937
                     /locus_tag="ACHW42_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13083..14120)
                     /gene="iap"
                     /locus_tag="ACHW42_000904"
     CDS             complement(13083..14120)
                     /gene="iap"
                     /locus_tag="ACHW42_000904"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR8942837.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14372..15280
                     /gene="cysD"
                     /locus_tag="ACHW42_000905"
     CDS             14372..15280
                     /gene="cysD"
                     /locus_tag="ACHW42_000905"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR8942838.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15282..16709
                     /gene="cysN"
                     /locus_tag="ACHW42_000906"
     CDS             15282..16709
                     /gene="cysN"
                     /locus_tag="ACHW42_000906"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR8942839.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16709..17314
                     /gene="cysC"
                     /locus_tag="ACHW42_000907"
     CDS             16709..17314
                     /gene="cysC"
                     /locus_tag="ACHW42_000907"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR8942840.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17364..17687
                     /locus_tag="ACHW42_000908"
     CDS             17364..17687
                     /locus_tag="ACHW42_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR8942841.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17881..18192
                     /gene="ftsB"
                     /locus_tag="ACHW42_000909"
     CDS             17881..18192
                     /gene="ftsB"
                     /locus_tag="ACHW42_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR8942842.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18211..18921
                     /gene="ispD"
                     /locus_tag="ACHW42_000910"
     CDS             18211..18921
                     /gene="ispD"
                     /locus_tag="ACHW42_000910"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR8942843.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18921..19400
                     /gene="ispF"
                     /locus_tag="ACHW42_000911"
     CDS             18921..19400
                     /gene="ispF"
                     /locus_tag="ACHW42_000911"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR8942844.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19397..20446
                     /gene="truD"
                     /locus_tag="ACHW42_000912"
     CDS             19397..20446
                     /gene="truD"
                     /locus_tag="ACHW42_000912"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR8942845.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20427..21188
                     /gene="surE"
                     /locus_tag="ACHW42_000913"
     CDS             20427..21188
                     /gene="surE"
                     /locus_tag="ACHW42_000913"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR8942846.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21182..21808
                     /gene="pcm"
                     /locus_tag="ACHW42_000914"
     CDS             21182..21808
                     /gene="pcm"
                     /locus_tag="ACHW42_000914"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR8942847.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21993..23087
                     /gene="nlpD"
                     /locus_tag="ACHW42_000915"
     CDS             21993..23087
                     /gene="nlpD"
                     /locus_tag="ACHW42_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR8942848.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23150..24142
                     /gene="rpoS"
                     /locus_tag="ACHW42_000916"
     CDS             23150..24142
                     /gene="rpoS"
                     /locus_tag="ACHW42_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR8942849.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24262..24669)
                     /locus_tag="ACHW42_000917"
     CDS             complement(24262..24669)
                     /locus_tag="ACHW42_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFR8942850.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24816..25409
                     /locus_tag="ACHW42_000918"
     CDS             24816..25409
                     /locus_tag="ACHW42_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFR8942851.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25409..26356
                     /locus_tag="ACHW42_000919"
                     /pseudo
     CDS             25409..26356
                     /locus_tag="ACHW42_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26629..27192)
                     /locus_tag="ACHW42_000920"
     CDS             complement(26629..27192)
                     /locus_tag="ACHW42_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942852.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27357..27608
                     /locus_tag="ACHW42_000921"
     CDS             27357..27608
                     /locus_tag="ACHW42_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8942853.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27610..29706
                     /locus_tag="ACHW42_000922"
     CDS             27610..29706
                     /locus_tag="ACHW42_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFR8942854.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7295 a         7383 c         8062 g         7058 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca
      541 tgctgggcgg tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg
      601 actacaacgt tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca
      661 acaaaatcat ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca
      721 ttggtaaaca tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg
      781 aagggctgaa aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga
      841 ttgaaaaata atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct
      901 caggcgagaa gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg
      961 tgcgaatgga tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg
     1021 gcgcacacct gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta
     1081 cgcccgccat gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg
     1141 gtttgtttct tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa
     1201 tcttcacctt cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca
     1261 cgccagagca tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact
     1321 cagtaggtca caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg
     1381 gcgcattcag cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact
     1441 ttatttccga aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt
     1501 tgttgattac gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg
     1561 ggcgttttgc agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg
     1621 gcgatgaagt ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact
     1681 ggcggcgcac ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga
     1741 tttatacctg gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca
     1801 gcctgatgct taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc
     1861 tgctggcaca tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag
     1921 taatggcctg tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca
     1981 ccaccatttc ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg
     2041 acattcgctc gctgggagtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa
     2101 gtactggcct gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc
     2161 tagcgacagt gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag
     2221 ccctcccgct ggtggcggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt
     2281 aaagccgaac cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt
     2341 aatagcggac cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct
     2401 gccgcgctgc tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc
     2461 atgggggatg aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa
     2521 gctgtattgc tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat
     2581 attgccgaat ggcagcgtca gaatccgctg gatctgataa ttaccggcag ccaaagcagc
     2641 gaagggcaga atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc
     2701 acccaggtgg agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa
     2761 catgggttgc gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt
     2821 gaagtggcct tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt
     2881 atccgtaaaa ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca
     2941 gagcaacgac gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag
     3001 ctatggcagg actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa
     3061 atcaggagaa tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc
     3121 tggcgcactg gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg
     3181 tggaacagtg gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg
     3241 atgaactgtc gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt
     3301 cgttagatat tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga
     3361 cgctgcaaac agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga
     3421 aaaacgccac gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg
     3481 actggctggt tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca
     3541 gacgggtgct ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg
     3601 cagaaaagct gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg
     3661 atgctgaaaa agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg
     3721 ttggcgcttc cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg
     3781 cgattaatca tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg
     3841 actggaaagt ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg
     3901 ctgccggatg atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt
     3961 taagcgtcgc atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa
     4021 ttcaattggt tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta
     4081 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
     4141 cataccagcc cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact
     4201 ttgggcatgc agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta
     4261 tcaatgttgt gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact
     4321 tccgctcttg ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc
     4381 accagtttca aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca
     4441 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
     4501 atattgccgt acaacacgga aataaactgg cattcagaat cgatggccag ttgcaggctg
     4561 gcattatctt taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata
     4621 aattatcctt attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg
     4681 ccactgttgt agtgtggatt cgtcgcacag caaacggcag gtgccacact ccaggcagcc
     4741 gcgatagtca atacgtaagt caccttcctg tgtgagagaa aaaagaccta ccggacaggc
     4801 gttaatcaac cgttgtacca tctgacgctc aacggagtca gccggaacaa tgtgcggcgc
     4861 atcagcaacg cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc
     4921 gccagatgcc acaggccatg acgacgtaaa tgatgccaga ataactggcg cagcggtgga
     4981 acaggtttat caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac
     5041 gcccgatacc tgccagggcg ttgcaaaagc gccggaacat gctgataacg ttgtagaaca
     5101 tcccatagca ggctgcgctc tacgttgtga tgataaagcg caaacagatt ttgcggctcg
     5161 cggtgctggc aggcgcttat tagcgtttgt gccgccgcct gtgcgccagt cagcgccata
     5221 tccatgccgc gcacggaaat tccggtattg acgcaactgc gcaacgcatc gcccaccagc
     5281 agccagccgt taccggcgta ttgcaccggc atactgtgca agccaccttc tggcaccaga
     5341 tgcgcaccat actccagtga ttccgtgttt ttgataagcg tgcgcactgc cggatgcgct
     5401 ttaaagcgag tcagcagctc gcttgccgga acacgacttt gcgtaaggga agagagcggg
     5461 caaacaatcc ctaacgagag cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag
     5521 tcatcacaga ttccgccgct gaacagcaac gctgcgcctt cgttattctc cagatgaaaa
     5581 cgttcttcaa tagcggatgt ttccagcgac agcacttctt tgatccccaa cgccatcgct
     5641 tcgccagcag gacgagtcac taacccgtga cgttcagcca ggacgctgtt ggcaccttct
     5701 gccagcacca cataacgggc gcggagaata tcgtcaccac aaataacgcc acagactctg
     5761 ccgttttctt catacagtgc atccaccgtc gctccgggga tgcattcgac accttctttt
     5821 tcggcttcgg caaccagcca cggatcgaac cgtgcacgta atacattcca ggattcaccg
     5881 ccgggctgta agctggaaaa cgtcgttgcg ccatccggcg ttaacaggga aaggctttcg
     5941 tgagtgatgc gtcgttcaag aggcgcggtc agatgaaatt gcgggaggag ttccgcgagt
     6001 gcatgggtat ataaccgcct gccggaaaga tttttgctgc cggggatttc agcgcgttcg
     6061 agtaacaaaa cggataaatc cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata
     6121 ccggcaccaa taataataat gtcgcagtcg tcttccatgt catgccccag atatccagaa
     6181 cacaacaccc taacatagcg ttactaaagg gaaattgacc gccgacacac tcttatcatt
     6241 cgccgcgata gatacaccct gcggtgcagg tttcttttac catcaccgca cttaacagcg
     6301 gcacaacggg tttaacctga tcccaaatcc attttgctaa aacttcgctg gttggatttt
     6361 ccagacctgg aatatcattg agataatggt gatcgaggcg ctcataagtt ggcttaaacg
     6421 ccgctttcag ttcagcgaaa tcgataatcc agcccgtatg cggatcgact tccccggtaa
     6481 tttccagtcg caccataaag gaatgcccgt gcaggcgacc acatttatgc ccttccggga
     6541 catgtggtaa gcggtgagcg gcttcgaagg tgaaatcttt aaataacgtg gtggacatca
     6601 taatttctct acaggacgat aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat
     6661 tatctaaaac gactttgttg cgcaaaatcg ctgatttatc ttaatgattg gctaaattca
     6721 tttgtttttc attaggttgg ttaatctatt ttgttgttaa agactattgc taaaacaggt
     6781 tagtcgattt ggttattagt tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt
     6841 aaccttacct tctcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac
     6901 tggaacataa cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc
     6961 ggagcaactg gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg
     7021 ggtttctggc tatttctggg gcgtgctcaa ccagcagcct gttgcgcttg cagtgacgcc
     7081 agcgcccgcc gcagaaatgc cgggtataac gattatctct gcttcgcaaa ccggcaatgc
     7141 gcgccgggtt gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct
     7201 ggtgaacgcg ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt
     7261 gacgtcaacg caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct
     7321 gttctccaaa aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga
     7381 tagctcttat gaatttttct gccagtccgg gaaagatttt gacagcaagc tggcggaact
     7441 ggggggtgaa cgcctgctcg accgtgtcga ttctgatgtt gaataccagg ctgctgccag
     7501 cgagtggcgc gcccgcgtgg ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc
     7561 gcaatccgtc gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc
     7621 gccgctggtg gctagcctct ctgttaacca gaaaattact gggcgtaact ctgaaaaaga
     7681 cgttcgccat atcgaaattg acttaggcga ctcgggcctg cgttaccagc cgggtgacgc
     7741 gctgggcgtc tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg
     7801 gctgacaggc gatgaacctg tcactgtcga gggcaaaacg ttgcctctga acgaagcgct
     7861 acagtggcac ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct
     7921 tacccgcagt gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc
     7981 cgcgacgacg ctgattgttg acatggtgcg tttctccccg gcacagcttg atgccgaagc
     8041 gctaattaat ctgctgcgcc cgctgacccc gcgtctctat tccattgcct cctcgcaggc
     8101 ggaagtcgag aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg
     8161 cgcccgtgcc ggtggtgcct ccagcttcct cgctgaccgc gtggaagaag agggcgaagt
     8221 ccgcgtattt atcgaacata acgataactt ccgcctgccc gctaacctgg aaaccccggt
     8281 gattatgatt ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc
     8341 cgctgacgaa gcgccaggta aaaactggct gttctttggc aatccgcatt ttacggaaga
     8401 cttcctgtat caggtggagt ggcagcgcta cgtcaaagag ggtgtgctga cgcggatcga
     8461 tctcgcctgg tcgcgcgacc aaaaagaaaa agtttacgta caagataaac tgcgcgaaca
     8521 gggcgcggag ctgtggcgct ggatcaatga cggtgcccac atttatgtct gcggcgacgc
     8581 taatcgcatg gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg
     8641 catggacacc gaagcggcgg atgaattttt aagtgagctg cgcgtagagc gccgttatca
     8701 gcgagatgtc tactaatgag cgaaaaacat ccagggcctt tagtggtcga aggaaaactg
     8761 acagacgccg agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat
     8821 ttaaacgacg gtctgaccgg cggctttaag ggcgataact tcctgctgat ccgcttccac
     8881 ggcatgtatc agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag
     8941 ccgcgccacg cgatgctgtt gcgctgccgt ctgccgggtg gagttatcac caccaaacag
     9001 tggcaggcga tcgataaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc
     9061 aaccgccaga cgtttcagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag
     9121 atgctgcact cggtcggtct tgatgcgctg gcgactgcta acgacatgaa ccgtaacgta
     9181 ctctgcacct cgaaccctta cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag
     9241 aagatttctg aacatctgct gccgcgtacc cgcgcgtatg cggagatctg gctcgatcag
     9301 gaaaaagtcg ccaccaccga tgaagaaccg atccttggtc agacttacct gccgcgtaag
     9361 ttcaaaacca cggtagtgat cccgccgcag aacgatattg atctgcacgc caacgacatg
     9421 aacttcgtgg cgatcgcgga aaacggcaag ctggtgggct tcaacctgct ggtgggcggt
     9481 gggctttcta tcgaacacgg taacaagaaa acctacgccc gcacggcgag tgagtttggc
     9541 tatctgccgc tagagcatac gctagcggtg gcggaagccg tcgtgacaac tcagcgtgac
     9601 tggggtaacc gaaccgatcg taaaaatgcc aaaaccaaat acacactgga gcgcgtgggg
     9661 gttgagacgt ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgc
     9721 ccgtatgaat ttacaggacg cggcgatcgt attggctggg ttaagggcat tgatgataac
     9781 tggcacctga cgctgtttat cgaaaatggt cgcatccttg attatccggg gcgtccgctg
     9841 aaaactggcc tgctggagat cgcgaagatc cacaaaggtg atttccgcat tacggcgaac
     9901 cagaatctga tcatcgccgg tgtgccggaa agcgagaaag cgaagatcga gaaaattgcc
     9961 aaagagagcg ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg
    10021 tcattcccga cttgcccgct ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc
    10081 gacaacatcg ataatttaat ggtgaaacat ggtgtcagcg atgagcatat cgtgatgcgt
    10141 gtaacaggct gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cctggtgggt
    10201 aaagcaccgg gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca
    10261 cggatgtata aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata
    10321 gggcgctggg cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg
    10381 ggcatcattc gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg
    10441 tcttgtaggc ctgataagac gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc
    10501 tatgtccaaa ctcgatctaa acgccctgaa cgaactgccg aaggtagatc gcattctggc
    10561 gctggcagaa actaacgccg aactggaaaa actggacgct gaaggccgcg ttgcctgggc
    10621 gctggataat ctgcccggtg aatatgtact ttcttccagc tttggcattc aggcggcggt
    10681 gagcctgcat ctggtgaatc agattcaccc ggatattccg gtgatcctca ccgacaccgg
    10741 ctatctgttc ccggaaacct accgctttat tgacgagtta acggacaaac tcaagctcaa
    10801 cctgaaagtg taccgcgcta ccgaaagcgc cgcctggcag gaagcacgct acggcaaact
    10861 gtgggaacag ggcgttgaag gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat
    10921 gaaccgggcg ctgaaagaac tgaatgcgca aacgtggttt gctggcctgc ggcgtgaaca
    10981 atccggcagc cgtgccaatt taccggtgct ggcaattcag cgtggcgtat ttaaagtgct
    11041 gccgattatc gactgggata accgaactat ttatcagtac ctgcaaaaac atggcctgaa
    11101 atatcaccca ttatgggacg aaggatattt atcggtgggc gatacccata caacccgtaa
    11161 atgggaaccc ggcatggcgg aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg
    11221 attacacgaa gggtaaatat tgccggataa attaaacgcc tctgtcagaa atgatggggg
    11281 cgtttttatt ttgcgagcag cattccggca tttaaaggtg aacatcgcag ggaaaattaa
    11341 caggtataga atggcgttgg gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc
    11401 agatggacaa aagccccagg cgatatttct atcaacctga ggctcgcgtt cgaacgtagc
    11461 aattcggatg ttagtctctt ctttgcaggg aggcaagaca tgctgacaaa atatgccctt
    11521 gtggcaatca tcgtactgtg ttgtacagta ctgggattca cgttgatggt aggtgactcg
    11581 ttatgtgagt tgagtatcag agaacgtggt atggagttta aggcagttct cgcttacgaa
    11641 tcgaagaagt agccaccacg cgggggtaaa accccgcgta ctgaattgtg atggtttggt
    11701 ctcctgcacc ctttttttaa atttatattt tttatgtgaa tatatagtct ctatttatta
    11761 ttatgaattt caaatgaaaa tcattaattt attcataatg taaatgtttt attggctgat
    11821 atatatcata gatagttttt aaaatatgcg taaatatcct ttaagtttac tgaaggataa
    11881 aaatattgtg actttctttg atttctgggg aaaaaaccga cgtggcgaga aagagggtat
    11941 cgacgactat caccttcttt gctggcattt gctggatgtg gccgcaatgg gctatttaat
    12001 ggttaaaaga aattgcttcg ggctggctga ttattttcgt caattaggga tttctgacaa
    12061 ggaacaggca gctcgatttt tcgcctggtt gctgtgctgg cacgatattg gaaaatttgc
    12121 ccgctctttt caacaacttt acctgccccc tgaattcaag attcaggaag gtgccagaaa
    12181 aaattacgaa aaaatctctc attcaacgct gggctactgg ttgtggaatc attatttaag
    12241 tgaatgtcag gagttacttc cttcatcttc gctctctccg cgcaaactta ggcgagtgat
    12301 agaaatgtgg atgcccgtga cgacagggca tcatggtcgg ccccctgatc gtatggatga
    12361 attggataat tttctgcctg aagacaaagc tgcagcacga gattttctcc ttgaaatcaa
    12421 gccactgttt ccgcgaatag agatccccac attctgggat gatgacgagg gcgttgaact
    12481 tataaaacaa ctttcctggt atatctctgc aaccgtcgta cttgcagact ggacagggtc
    12541 gtcaacgcga ttttttccac gcgtcgcaca ggcaatggat attgaagatt actggcaaaa
    12601 ggctttagtt caggctcaaa acgcattaac cgtctttccg ccacaagcag aaaccgcgcc
    12661 tttcaccgga attaatacgc tgtttccttt tattgagaat acgacaccgt tacagcaaaa
    12721 ggtgctggat ctggatatca atcagccagg accacagttg tttattctgg aagatgtgac
    12781 cggcgcaggt aaaacagaag cggcgcttat cctggcgcac aggttgatgg ctgcgggtaa
    12841 cgctcagggg ctgttttttg gcctgccaac aatggcaacg gccaatgcca tgtacgatcg
    12901 actggtcaaa acctggcttg ctttctattc gccagaggtg ttccccgctc cagcggagat
    12961 aaaccggcgc aatacggtgc ggttttcccg gaagtaaacc ggacgcactg gatgcgatga
    13021 tgggtatcac ttagaattcc ccgcccctgc ggtagaactc ccagctccca ttttcaaacc
    13081 catcaagacg ccttcgccag ctccttcacc aacggtagca ttatccgcat aacgtcacgg
    13141 cagcgacgtt ctattcttcc aggaagtgcc ttatcaatat gttgttgatt atccagtctt
    13201 acgtcatgcc agctatttcc cgccgggaag gcaggtgttt ttgcgcgttg ctgataacca
    13261 tccttatttc caagattcca gttagtcgct tccaccgaaa gtacagcaat gcccgctttg
    13321 tcgaatattt ctgcgtcatt acaaccccca gtgcctttcg gataattttt attcaaaccc
    13381 ggattggtcg ttgcggctat tccgtgactg cgtgcaattg ccagcgccct gtcgcgcgtt
    13441 aatttcctta ctgcttcagg ggtttttaca ccgctgttga aatacaattt atcgccaaca
    13501 attaagttat cgagattaat caccagcagc gtatttttct tttcggtgtc actcatccgc
    13561 ttgagtaaat tctcagcgcc taattttcct tcctcttcgc cgctggtcgc cacaaatcga
    13621 ataccatact cggtaggcgt atttttcagg cgttctgcca gttccagcat gacacctaaa
    13681 cctgcggcgt tatcatctat tccttgtaac gtcagtccgc cgagattggc atctgcgtca
    13741 gcatcgctca gcggggcgta agtatccaga tgcgccataa tgatgatctg ctgcggcgct
    13801 ttgccttcat gagcggcaat caccgtactt cctgtcacgt tatgccagtt cttgcgatta
    13861 tcgcgggcgg tataaatata ccgactatta aatgtccgaa tatcactgcg ataacccatt
    13921 tgctgaaact gttggcgaat ataatcggca gataacattt ctgctggagt tccggtcatg
    13981 cgtcccggaa agaaagtagc aatatgtcgt gcctgggtat tagcaaaatc gccaggttta
    14041 ggtgacgagg cgtgtacggg gagaataaag catacgccga gcgccagggc agcggtacgg
    14101 tggcgcaatg cggaaaacat agtgagtcct taaataccat gcaaattttt ttaccgccat
    14161 agtatgaaac tgccgctgcg ctaaaacaat ttcaaatctt cctaaacgcc cgaaatccgg
    14221 tgccttaagc actttttgat attagctttg ccaaatcgtt attccgttaa ggaactactc
    14281 attctaattg gtaatttcat tcgttctctt acgctcccta tagtcgaaac atctgatggc
    14341 aagaaaatag cggtattgca aaggaacggt tatggctcaa aaacgactta ctcacctgcg
    14401 gcaactggag gcggaaagca tccacattat tcgcgaggtg gcggcagaat tctcaaatcc
    14461 ggtgatgctc tactctatcg gtaaagattc cagcgtcatg ctgcatctgg cgcgcaaggc
    14521 gttttatcca ggtacgctgc ctttcccgtt gctgcatgtc gataccggct ggaaattccg
    14581 cgagatgtat gagttccgcg atcgtacggc gaaagcctac ggctgcgagc tgctggtgca
    14641 taaaaacccg gaaggcgtgg cgatggggat taatccattc gtgcacggca gcgcgaaaca
    14701 taccgatatt atgaaaactg aaggcctgaa acaggcgctg aacaaatacg gttttgatgc
    14761 cgccttcggt ggtgcgcgcc gtgacgaaga gaaatcccgc gctaaagagc gaatctactc
    14821 tttccgtgac cgcttccatc gctgggatcc gaaaaatcag cgcccggagc tgtggcacaa
    14881 ctacaacggg caaattaaca aaggcgaaag catccgcgtc ttcccgctct ctaactggac
    14941 cgagcaggat atctggcaat acatctggct ggaaaatatc gacattgtgc cgctgtatct
    15001 cgctgcggaa cgtccggttc tggaacgcga cggtatgttg atgatgattg atgacaaccg
    15061 tatcgacctg caaccgggcg aagtgatcaa aaaacggatg gtgcgtttcc gtacgctggg
    15121 ctgctggccg ctgaccggtg cggtggagtc aaatgcacaa acactgccgg aaatcatcga
    15181 agagatgctg gtttccacca ccagtgaacg tcagggtcgc gtgattgacc gcgaccaggc
    15241 gggatccatg gagctgaaaa aacgtcaggg gtatttttaa gatgaacacc gtacttgcac
    15301 aacaaatcgc caatgaaggc ggcgtcgaag cctggatgat tgcgcaacaa cataaaagcc
    15361 tgctgcgttt tctgacctgt ggtagcgtcg atgacggcaa aagtaccctg attggtcgtc
    15421 tgctgcacga tacccgccaa atctacgaag atcagctctc atcgctgcat aacgacagta
    15481 agcgtcacgg aacccagggc gaaaagctgg atctggctct gctggtggac ggcctgcaag
    15541 ctgagcgcga acagggcatc actattgatg tggcctaccg ctatttctct accgagaagc
    15601 gtaaatttat tatcgccgac accccagggc acgagcagta cactcgcaat atggcgactg
    15661 gcgcatcgac atgtgaactg gcgatcttac tgattgatgc ccgtaaaggc gtgctcgatc
    15721 aaacccgtcg tcacagtttt atctccacac tgttggggat caaacatctg gtcgtggcga
    15781 tcaacaaaat ggatctggtg gattacagtg aagagacgtt cacccgtatt cgtgaagatt
    15841 atctgacctt tgccgggcag ctgccgggta atctggatat ccgctttgtg ccgctctccg
    15901 cactggaagg tgacaacgtg gcttcgcaaa gtgaaagtat gccgtggtac agcggtctga
    15961 cactgctcga agtgctggaa accgtggaga tccagcgagt ggtggatgcc cagccaatgc
    16021 gcttcccggt gcagtacgtt aaccgcccaa atctcgattt tcgtggctac gccggaatgc
    16081 tggcatccgg tcgcgtggaa gtcgggcaac gtgtaaaagt gctgccctct ggtgtggaat
    16141 caaacgtcgc gcggatcgtg acttttgatg gtgatcgcga agaagtcttt gccggagaag
    16201 cgatcaccct ggtgctgacg gatgagatcg atatcagccg tggtgatctg ctgctggcgg
    16261 cagacgaagc gttaccggcg gtgcagagtg cgtcggtgga tgtggtatgg atggcggaac
    16321 agccgctttc cccgggccag agttatgaca tcaaaattgc cggtaagaag acgcgtgctc
    16381 gtgttgatgg cattcgttat caggttgata ttaataacct tacccaacgc gaagttgaaa
    16441 acctgccgct gaacggcatc ggcctggtgg atctcacttt tgacgagcca ctggtgttag
    16501 atcgctatca gcaaaatccg gttaccggtg ggctgatttt tatcgatcgc ctgagcaatg
    16561 tgaccgtggg tgccggtatg gtgcacgagc cagttagcca ggcaacagct gcgtcatctg
    16621 aattcagtgc attcgaactg gaattgaatg ccctggttcg tcgccacttt ccgcactggg
    16681 gcgcgcgcga tttgctgggg gataaataat ggcgctgcat gacgaaaacg tcgtctggca
    16741 tagtcatccg gtcactgtgc aacaacgcga actacaccac ggtcatcgtg gtgtagtgct
    16801 gtggtttacc ggcctctccg ggtccggtaa atcaacggtc gccggggcgc tggaggaggc
    16861 gttacataaa ctcagcgtca gtacgtatct gctggatggc gacaatgttc gccacggatt
    16921 atgcagcgat ctcggtttta gcgatgccga tcgtaaagag aatatccgtc gcgtcggtga
    16981 agtggcgaat ttgatggttg aagccggact ggtggtgctg accgcattta tctcgccaca
    17041 ccgcgccgaa cgccagatgg ttcgcgaacg cgtaggagaa gggcgcttta ttgaagtgtt
    17101 tgtcgatacg ccgctggcga tttgcgaagc ccgcgatcca aaaggcttat ataagaaagc
    17161 gcgtgccggt gaactgcgca actttacggg aatagattcc gtttacgaag cgcctgaatc
    17221 ggcagaaatt catctcaatg gtgaacaatt agtaacaaat ttggtacagc aattattaga
    17281 tctgttgaga cagaacgata ttatcagatc ctgagacacc accgggtttc catgcccggt
    17341 tcgtcaaagt aacaggatca aatatgcgta atagccataa cattacacta acaaataacg
    17401 acagccttac tgaggatgaa gaaactacat ggtcactgcc tggtgccgtg gtcggtttta
    17461 tctcctggtt atttgcgctg gcgatgccga tgttgattta tggctctaac acgctgttct
    17521 tctttctcta cacctggcct ttctttctgg cactgatgcc tgtcgcggta gtggtgggga
    17581 ttgcgctgca ttcattgatg gacggaaagc tacgctacag tattgttttc accctggtga
    17641 cagtaggcat tatgtttggc gcactgttta tgtggctact gggctaattt gtactttctt
    17701 gtctcctctg ttcataattc aaaccgtaac taataatgag attatgttct gcacgccctg
    17761 ggtatacgta acaatgggca aatgtggtac atttgcccgc gttgtcgcgg tatccccaac
    17821 agaggatgta gagtcgtctt cggatgcatg ggatgatgat gcagtttttc agggggcagg
    17881 atgggtaaac taacgctgct gttgctggct attctggtct ggctacagta ttcgctgtgg
    17941 ttcggtaaga acggtataca tgactatacc cgcgtcaatg atgatgtggc ggcactgcaa
    18001 gctacaaacg cgaaacttaa agcgcgaaac gatcaacttt ttgccgaaat tgacgatctc
    18061 aatggcggcc aggaggcgct cgaagagcgt gcgcgtaatg aactcagcat gaccaggccg
    18121 ggcgaaactt tttatcgtct ggtgcctgac gcgtcgaagc gcgcacagtc tgcggggcaa
    18181 aacaatcgat aaatcagccc gggaattaac atggcaacca ctcatttgga tgtttgcgcc
    18241 gtggttccgg cggccggatt tggccgtcga atgcaaacgg aatgtcctaa gcaatatctc
    18301 tcaatcggta atcaaaccat tcttgaacac tcggtgcatg cgctgctggc gcatccccgg
    18361 gtgacacgtg tcgtcattgc cataagtcct ggcgatagcc gttttgcaca acttcctctg
    18421 gcgaatcatc cgcaaatcac cgttgtagat ggcggtgatg agcgtgccga ttccgtgctg
    18481 gcaggtctga aagccgctgg cgatgtgcag tgggtattgg tgcatgacgc cgctcgtcct
    18541 tgtttgcatc aggatgacct tgcgcgattg ttggcgttga gcgaaaccag ccgcacggga
    18601 gggatcctcg ccgcaccagt gcgcgatact atgaaacgtg ccgaaccggg caaaaatgcc
    18661 attgctcata ccgttgatcg caacggctta tggcacgcgc tgacgccgca atttttccct
    18721 cgtgagctgt tacatgactg tctgacgcgc gctctaaatg aaggcgctac cattaccgac
    18781 gaagcctcgg cgctggaata ttgcggattt catccgcagt tggtcgaagg ccgcgcggat
    18841 aacattaaag tcacgcgccc ggaagatttg gcactggccg agttttacct cacccgaacc
    18901 atccatcagg agaatacata atgcgaattg gacacggttt tgacgtacac gcctttggcg
    18961 gtgaaggccc aattatcatt ggtggcgtac gcattcctta cgaaaaagga ttgctggcgc
    19021 attctgatgg cgacgtggcg cttcatgcgt tgaccgatgc attgcttggc gcggcggcgc
    19081 tgggggatat cggcaaactg ttcccggata ccgatccggc atttaaaggt gccgacagcc
    19141 gcgagctgct acgcgaagcc tggcgtcgta ttcaggcgaa gggttatacc cttggcaacg
    19201 tcgatgtcac tatcatcgct caggcaccga agatgttgcc gcacattcca caaatgcgcg
    19261 tgtttattgc cgaagatctc ggctgccata tggatgatgt taacgtgaaa gccactacta
    19321 cggaaaaact tggatttacc ggacgtgggg aagggattgc ctgtgaagcg gtggcgctac
    19381 tcattaaggc aacaaaatga ttgagtttga taatctcact tacctccacg gtaaaccgca
    19441 aggcaccggg ctgctgaaag ccaatccgga agactttgtg gtggtggaag atttgggctt
    19501 tgagcctgat ggtgaaggtg agcatattct ggttagaatc ctcaaaaacg gctgcaatac
    19561 ccgttttgtg gcggatgcac tggcgaaatt cctgaaaatt cattcccgtg aagtcagctt
    19621 cgctgggcaa aaagacaaac atgctgtaac ggaacagtgg ttatgtgctc gcgtgccggg
    19681 caaggaaatg cccgatctga gcgcctttca actggaaggc tgccaggtgc tggagtatgc
    19741 gcggcacaag cgcaagctgc gtttaggggc gctgaaaggt aacgccttta ccctggttct
    19801 gcgcgaagtg agcaatcgcg atgacgttga acaacgtctg atcgatattt gcgtaaaagg
    19861 tgtaccgaac tacttcggtg cccaacgttt tgggattggc ggtagcaact tgcagggcgc
    19921 gctgcgctgg gcgcaaacca atactccggt gcgcgatcgc aataaacgga gtttttggtt
    19981 gtcggcagcc cgcagtgcgt tgtttaatca gattgttgct gagcgcctca aaaaagcaga
    20041 cgttaatcaa gttgttgacg gcgatgcgct acaattagcc ggacgtggta gctggtttgt
    20101 agcaaccacc gaagaactgg cggaattaca gcgtcgcgtc aacgataaag agctgatgat
    20161 aaccgccgca ttgccgggca gtggcgaatg gggaactcag cgtgaagcgc tggcattcga
    20221 acaagcagct gtcgccgcag aaactgaatt acaagcttta ctggtgcgcg aaaaagttga
    20281 agccgcgcgc agagcgatgc tgctgtatcc gcaacaatta agctggaact ggtgggatga
    20341 cgtcaccgta gagatccgtt tctggcttcc ggcgggtagt tttgcaacca gcgttgtcag
    20401 ggaacttatc aacacaacag gtgattatgc gcatattgct gagtaattat gacggggtac
    20461 atgcacccgg tatacaaacg ctggcgaaag ccttgcgtga gtttgctgac gttcaggtgg
    20521 tcgcccccga tcgtaaccgc agcggcgctt caaattctct gacactggaa tcctccctgc
    20581 gcacgtttac ctttgaaaat ggtgatattg ctgtgcaaat gggaaccccg accgattgtg
    20641 tctatcttgg cgtgaatgct ctgatgcgtc cgcgcccgga cattgttgtg tccggaatta
    20701 acgccgggcc gaatctgggg gatgatgtta tttattccgg tacggtagcc gccgcgatgg
    20761 aaggccgtca tttaggtttt ccggcgctgg ccgtctcgct tgacgggcat aaacattacg
    20821 acactgccgc ggcggtaacc tgttcaattt tgcgcgcact gtgtaaagag ccgctgcgca
    20881 ccggacgtat tcttaatatt aacgttcagg atttaccctt ggatcaaatc aaaggtattc
    20941 gcgtgacgcg ctgcggtaca cgacatccgg cagatcaggt gatcccgcag caagatccgc
    21001 gcggcaatac gctgtactgg attggcccgc cgggcggtaa atgtgatgcc ggtccgggga
    21061 ccgattttgc tgcggtagat gagggctatg tctccatcac gccgctgcat gtggatttaa
    21121 ctgcgcatag cgcgcaagat gtggtttcag actggttaaa cagcgtggga gttggcacgc
    21181 aatggtaagc agacgcgtac aagcacttct ggatcaatta cgtgcgcaag gtattcagga
    21241 tgagcaggtg ctgaatgcac ttgccgccgt gccgcgtgaa aaattcgttg atgaagcgtt
    21301 tgaacaaaaa gcctgggaca atatcgcctt gccgataggt caggggcaga caatttcgca
    21361 gccgtatatg gtggcgcgaa tgaccgaatt actcgagctg acgccgcagt cgcgggtgct
    21421 ggaaattggc actggttcgg gatatcaaac ggcaatcctg gcgcatcttg tccagcatgt
    21481 ttgctcggtt gaacggatta aaggcttgca gtggcaggca cgtcgccgcc tgaaaaatct
    21541 tgatttacat aatgtttcaa cccgtcatgg tgatggatgg caaggttggc aggcacgtgc
    21601 gccgtttgac gctatcattg ttacggcggc accgccggaa attccaactg cgctaatgac
    21661 gcagctggac gaaggcggga ttctcgtctt acccgtaggg gaggagcacc agtatttgaa
    21721 acgggtgcgt cgtcggggag gcgaatttat tatcgatacc gtggaggccg tgcgctttgt
    21781 ccctttagtg aagggtgagc tggcttaaaa cgtgaggaaa tacctggatt tttcctggtt
    21841 attttgccgt aggtcagcgt atcgtgaaca tcttttccag tgttcagtag ggtgccttgc
    21901 acggtaatta tgtcactggt tattaaccaa tttttcctgg gggataaatg agcgcgggaa
    21961 gcccaaaatt caccgttcgc cgcattgcgg ctttgtcact ggtttcgcta tggctggcag
    22021 gctgttctga cacttcaaat ccaccggcac cggtcagctc cgttaatggc aatgcgcctg
    22081 caaatactaa ttctggtatg ttgattacgc cgccgccgaa aatggggacg acgtctacag
    22141 cgcagcaacc gcaaattcag ccggtacagc agccacaaat tcaggctact caacaaccgc
    22201 aaatccagcc agtgcagcca gtagctcagc agccggtaca gatggaaaac ggacgcatcg
    22261 tctataaccg tcagtatggg aacattccga aaggcagtta tagcggcagt acctataccg
    22321 tgaaaaaagg cgacacactt ttctatatcg cctggattac tggcaacgat ttccgtgacc
    22381 ttgctcagcg caacaatatt caggcaccat acgcgctgaa cgttggtcag accttgcagg
    22441 tgggtaatgc ttccggtacg ccaatcactg gcggaaatgc cattacccag gccgacgcag
    22501 cagagcaagg agttgtgatc aagcctgcac aaaattccac cgttgctgtt gcgtcgcaac
    22561 cgacaattac gtattctgag tcttcgggtg aacagagtgc taacaaaatg ttgccgaaca
    22621 acaagccaac tgcgaccacg gtcacagcgc ctgtaacggt accaacagca agcacaaccg
    22681 agccgactgt cagcagtaca tcaaccagta cgcctatctc cacctggcgc tggccgactg
    22741 agggcaaagt gatcgaaacc tttggcgctt ctgagggggg caacaagggg attgatatcg
    22801 caggcagcaa aggacaggca attatcgcga ccgcagatgg ccgcgttgtt tatgctggta
    22861 acgcgctgcg cggctacggt aatctgatta tcatcaaaca taatgatgat tacctgagtg
    22921 cctacgccca taacgacaca atgctggtcc gggaacaaca agaagtgaag gcggggcaaa
    22981 aaatagcaac catgggtagc accggaacca gttcaacacg cttgcatttt gaaattcgtt
    23041 acaaggggaa atccgtaaac ccgctgcgtt atttgccgca gcgataaatc ggcggaacca
    23101 ggcttttgct tgaatgttcc gtcaagggat cacgggtagg agccacctta tgagtcagaa
    23161 tacgctgaaa gttcatgatt taaatgaaga tgcggaattt gatgagaacg gagttgaggt
    23221 ttttgacgaa aaggccttag tagaagagga acccagtgat aacgatttgg ccgaagagga
    23281 actgttatcg cagggagcca cacagcgtgt gttggacgcg actcagcttt accttggtga
    23341 gattggttat tcaccactgt taacggccga agaagaagtt tattttgcgc gtcgcgcact
    23401 gcgtggagat gtcgcctctc gccgccggat gatcgagagt aacttgcgtc tggtggtaaa
    23461 aattgcccgc cgttatggca atcgtggtct ggcgttgctg gaccttatcg aagagggcaa
    23521 cttggggctg atccgcgcgg tagagaagtt tgacccggaa cgtggtttcc gcttctcaac
    23581 atacgcaacc tggtggattc gccagacgat tgaacgggcg attatgaacc aaacccgtac
    23641 tattcgtttg ccgattcaca tcgtaaagga gctgaacgtt tacctgcgaa ccgcacgtga
    23701 gttgtcccat aagctggacc atgaaccaag tgcggaagag atcgcagagc aactggataa
    23761 gccagttgat gacgtcagcc gtatgcttcg tcttaacgag cgcattacct cggtagacac
    23821 cccgctgggt ggtgattccg aaaaagcgtt gctggacatc ctggccgatg aaaaagagaa
    23881 cggtccggaa gataccacgc aagatgacga tatgaagcag agcatcgtca aatggctgtt
    23941 cgagctgaac gccaaacagc gtgaagtact ggcacgtcga ttcggtttgc tggggtatga
    24001 agcggcaaca ctggaagatg taggtcgtga aattggcctc acccgtgaac gtgttcgcca
    24061 gattcaggtt gaaggcctgc gccgtttgcg cgaaatcctg caaacgcagg ggctgaatat
    24121 cgaagcgctg ttccgcaagt aagtaagcat ctgtcagaaa ggccagtctc aagcgaggct
    24181 ggcctttttc ttttgtttgg gaagcttgtg tttttgtcgc ctggataaga cacgtcagcg
    24241 tcctacccgg caatcagcat tctatgtgct catcatcttg cgcaccaact gcataaacag
    24301 ctcctgctct tctccactca aacgccccaa aaattcctca tccacgctgt caccaatagg
    24361 tatcgccgca gcaagtagct tttctccttc ggcagtcagc cagacaaagc gtcgccgctt
    24421 gtcagcgacg tcatgttcac gtctgactaa gccacgattt tccattcttg ccaacatttc
    24481 tgctagcgtt gctttggtgc tgactgctgc ttctatcagt gccacctgtt caataccagg
    24541 tttatcagca atggcgcgca tcaccgcgta ctgtggtttg gtcaggtcag gtaactcgtg
    24601 ctgccagcga gccgtgtgct gctgaaaaag ctgtcgtaac tgatggaacg ctttatttcg
    24661 taacgccatg taaactccgg gctaattctc tgtcgctatc atagcggttt tttagtgacg
    24721 aagaagatgt tgttactttt caatccttgc cgctgtggaa atgaaggagt atgttaataa
    24781 taacgttcgt atacgaataa ttaagagaat aagcaatgaa actgatcgtc gggatgacag
    24841 gggctaccgg tgcgcctctt ggtgtggcat tactgcaagc gctgcgtgag atgccgaatg
    24901 tcgagactca tctggtgatg tcgaagtggg cgaaaaccac cattgaactg gaaacgcctt
    24961 acagcgctcg cgatgttgct gccctcgcag acttcagcca taacccggcg gatcaggcgg
    25021 cgatcatctc atccggttct tttcgtaccg acggcatgat cgttattccg tgcagtatga
    25081 aaacgctcgc cggtatccgc gctggttacg ccgatggcct ggtagggcgc gcggcggacg
    25141 tcgtgctcaa agaaggccgc aaactggtgc tggtgccgcg tgaaatgccg cttagcacca
    25201 tccatctcga aaatatgctc gcactttcac gcatgggcgt ggcgatggtg ccgccgatgc
    25261 ctgcctttta taaccatccc gaaacggtag atgacattgt ccaccatgtg gtagcccgcg
    25321 tgctggatca atttggcctc gaacatcccc acgccaggcg ctggcaagga ttgccgcagg
    25381 cccggaattt ttctcaggag aatgaataat ggcatttgat gatttacgca gctttttaca
    25441 ggcgcttgat gaccacggcc agttactgaa aatcagcgaa gaagtgaacg ctgagccgga
    25501 tctggcagca gcagctaacg ccaccgggcg tatcggcgac ggcgcgcccg cgctgtggtt
    25561 tgataatatt cgcggcttta ccgatgcccg cgtggcgatg aataccatcg gttcctggca
    25621 gaaccacgcg atttcccttg gcctgccacc aaacaccccg gtcaaaaagc agattgatga
    25681 gtttatccgc cgctgggata acttcccgat tgccccggag cgccgcgcca atccagcctg
    25741 ggcgcagaac accgtcgatg gcgaagagat caacctgttc gatattctgc cgctgtttcg
    25801 cctgaatgat ggcgatggcg gtttctatct cgacaaagcg tgcgtggttt cccgcgatcc
    25861 gctcgacccg gataacttcg gcaagcagaa cgtcggcatc taccgcatgg aagtgaaggg
    25921 caagcgtaag ctcggcctgc aaccggtgcc gatgcacgat atcgccctgc atctgcataa
    25981 agcagaagag cgcggtgaag atctgccgat tgcgatcacg ctcggtaacg atccgatcat
    26041 cacgctgatg ggggccacgc cgctgaaata cgatcagtct gagtatgaaa tggcaggcgc
    26101 gctgcgtgaa agcccgtacc cgatcgccac cgctccgctg accggttttg atgtgccgtg
    26161 gggttcagaa gtgatcctcg aaggggttat cgaaagccgt aaacgcgaaa tcgaagggcc
    26221 gttcggtgag tttaccgggc actactccgg tgggcgcaac atgaccgtgg tgcgcatcga
    26281 taaagtctct tatcgcagca aaccgatttt cgaatcactc tatctcggta tgccgtggac
    26341 cgaaatcgac tacctatgac taggtaattt attcaagata accgggaaga gattaaaaaa
    26401 gacgggaata gattggaatc aacaagttag gaactcaagg ttttcaagat aaagcgagtc
    26461 caaaaatgtt aaacggttcg atatcaataa aacattcaag ataactcaca ggttcttaca
    26521 atagccttta aagaccgttc aaaaacgatt caggtgttta ttatagcgct gatgggtgca
    26581 agcgccaaaa ccaatgtaat tttagcacag ctaggcactt gcactgggtt atgcgctggc
    26641 tgctttctga ggtagattta agtgatcgcc cttagcttcg gacaacgcgt aaatctctct
    26701 aaccttttcg tctggcaaat taccaaggct taacgctatg gctctacgaa ctccaactag
    26761 agcgtgcaac tcttggatgt gctcatccag aagaatgagt aattgctccg ctccttttct
    26821 cccaagaaca tcagctactt gttgtttttc ttgtggttta agaaatgaca agaacattag
    26881 aatttgagca tcaacattat ccgcattctc ttttttgggt tctttggaat cttttagttc
    26941 aacatcaccc tctcccgtca tgagccattc caaggtaaca ttttcgcttt ctgctatctt
    27001 ttttaactga tacgggcgtg gtatcggtgc gtaatcccgc ctgttgtaat aattttttaa
    27061 agtgccttca ttgatccccc atgcccgagc agccgcattt cttgaagggt atcgttctac
    27121 taacctaaag attctttcga taatcggttc ttttccatcg gcaggaaaag aaccttgggg
    27181 tgcttttctc attcttggtt cctttctatc taattcattg ataaataata aaattaaaaa
    27241 ataaaccata aaaacaccaa agaacccttt gcagggaatc tttggtatct taatctttat
    27301 ccatacggat atccgaccgg atatccgcgc gggtaatttt gtaggttaaa ggaaagatga
    27361 gacaaagaga agatagtgac cgcgattggc cttcgctgaa gattcagctg gcgctcaaag
    27421 ataacgggtg gtcttccttc cgggagcttt cactccatgc tggttatgct caggattcac
    27481 tgaaagcggt agttcgcaca ccaaacaaaa aatacgaacg cgtaatttct gacgctatag
    27541 gtgtaccgcc agaaatcatc tggcccagcc ggtatcgtaa cgatggattt atcaggaagg
    27601 cgtcgtgata tgcagtcgta cgttaccgta aatgacctgc tcggcgtacc aggaatgcca
    27661 gccaccacaa aaggcatccg acaggcgtta cagcgttttt ctcgtgatct tggtgacgtt
    27721 agtcgccgtc gggaaggcac aaaagccatt gagtaccaca ttgattgtct gcctgaaata
    27781 acacgaaagg cactgcgtga acgctatgtg gaacagctgg tggctacaga gaacaatgtt
    27841 tctgaagtga aagctgttac cagaaaaaca cgcaatcctg acgctgtcca ggcaatcgaa
    27901 gcatatcgcg gttctccaca actgatggaa gaacgcctga atgcgctgac cgaaaaccag
    27961 cgctgggtat ctgaagcaag agctgcgctg gtggtggaag tgctgaagct ggaaagcgcc
    28021 ggtaaccccg ggcgactgaa agccattaac tttcttgttg aaaaagcccg taaaggtgag
    28081 ctgccggagc gcctgcaaca ggccgcagtt aacgccaatg caaaacgtgg cgctaatcgt
    28141 actatcagcc gcgatccact ttaccagtgg gttctgaaat acaaccagtc gcagaacgcc
    28201 gccgaacggc tgctgttact ggcaccggga aaacgcgacg aaataaaacc ggaagaaatc
    28261 agctggctgc ctgaatttct ggcgcagtat cgccaggtga acggcagacc catgtcagaa
    28321 gcctacgagg attttgtcgc agaatggcag cgtcgccatg cggacgaacc gtacatgctg
    28381 gaggtgatgc cttcctatga tgttgtccgg tatgccatga aaaaactgcc ggaagtggtg
    28441 aaacaaaaag ggcgcgttac cggcagtgaa taccgtcagc ttgaaggatt cacccgccgc
    28501 gactggacag ctatgccggt gaattatgtc tggattggtg acggtcacgg tatgaagctg
    28561 aaatgtgcgc atccgatcca cgggcgtcca ttctcaccgg aagtcacgtt tgtgatcgat
    28621 ggtggaacac ggtttgttgt tggctggagt cttgaccttg ccgaaaacgt gttcgccgtt
    28681 gccggtgcga tacagcacgg cattcgcaac cacggaaaac cgttcctgta ttactcggat
    28741 aacggttccg gtgaaacggc tgacatgctg gacaaagagg tcgtgggtat tctgccacgc
    28801 ctggggatta aacaccctac cggtattgcc ggtaatccac agggacgcgg cattattgaa
    28861 cgcctgaacc ggacactgcc gatgcgtata gccaggaggt atcgcaccta ttttggcaag
    28921 ggtgctgacc gggaatcgtt gcgtgtactt aaccgtgatc tgcgttcagc gtttaacgcc
    28981 ctgcaacagg acaagcctct gaacgaccgc cagaaagcgg caatgcgcga gctgccatcg
    29041 tgggcggagc ttattgaggc tattcgtgaa ggcgtggaat ggtacaacaa ccgcccgcat
    29101 tctgagctgc cgatgaaacc gaacggacgg cattacagcc cgacagagtt cagaaaaaaa
    29161 cgccaggcag aagaagacac cgaaattgag tggctgagtg atcttgagct gcgcgacatg
    29221 ttccgcccaa tggtggaacg accggtaaga cgctgcgaaa ttcagtggct gaacaatatt
    29281 tactacgcgc cggagttgcg tgacgaacac ggtcgcaaag tgcttatcag ttatgacatt
    29341 catgacgccg agcgaattac ggtacgtcgc aaggacggca gctttatctg tgaggcgata
    29401 tggaacggca ataaacgcgc agcctttgct gtcagtgctg aatatcacaa acaacagcag
    29461 cgtattaaag gaatgcgcaa acgcgctgag gaaaaaattc gtgatgctga agatgaaggt
    29521 attcagattc tggaacacaa acaggctgaa ccctggctga gtaatgttta tcgcccggtt
    29581 ggtaatgtgg tggcagttca gcaaccggaa tatgaagaag agcatgacga agaatttgaa
    29641 cgcgatttcc ggctggggat gcaaaaactt tttgccatgc aggaagaaga tgatccgctg
    29701 gcctgaataa aaaaacagtc cgcgggaacg gactgttaaa taaaggaaac aaagctgatt
    29761 tgaaataacc gaggaaatac tatgactgat attgatga
//
LOCUS       DAZSNM010000023        29466 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZSNM010000023 DAZSNM010000000
VERSION     DAZSNM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29466
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.023"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..213)
                     /locus_tag="ACHW42_000923"
     CDS             complement(<1..213)
                     /locus_tag="ACHW42_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HFR8942855.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="ACHW42_000924"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="ACHW42_000924"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR8942856.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="ACHW42_000925"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="ACHW42_000925"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR8942857.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="ACHW42_000926"
     CDS             2894..3634
                     /locus_tag="ACHW42_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR8942858.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="ACHW42_000927"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="ACHW42_000927"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR8942859.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="ACHW42_000928"
     CDS             complement(4192..4794)
                     /locus_tag="ACHW42_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR8942860.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4864..4929
                     /gene="yobI"
                     /locus_tag="ACHW42_000929"
     CDS             4864..4929
                     /gene="yobI"
                     /locus_tag="ACHW42_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFR8942861.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5068..5679
                     /gene="ruvA"
                     /locus_tag="ACHW42_000930"
     CDS             5068..5679
                     /gene="ruvA"
                     /locus_tag="ACHW42_000930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR8942862.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5688..6698
                     /gene="ruvB"
                     /locus_tag="ACHW42_000931"
     CDS             5688..6698
                     /gene="ruvB"
                     /locus_tag="ACHW42_000931"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR8942863.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="ACHW42_000932"
     CDS             complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="ACHW42_000932"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR8942864.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="ACHW42_000933"
     CDS             complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="ACHW42_000933"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR8942865.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8461..9393
                     /gene="znuA"
                     /locus_tag="ACHW42_000934"
     CDS             8461..9393
                     /gene="znuA"
                     /locus_tag="ACHW42_000934"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR8942866.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9409..10731
                     /gene="mepM"
                     /locus_tag="ACHW42_000935"
     CDS             9409..10731
                     /gene="mepM"
                     /locus_tag="ACHW42_000935"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR8942867.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10851..11822
                     /gene="lpxM"
                     /locus_tag="ACHW42_000936"
                     /gene_synonym="msbB"
     CDS             10851..11822
                     /gene="lpxM"
                     /locus_tag="ACHW42_000936"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR8942868.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="ACHW42_000937"
     CDS             complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="ACHW42_000937"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR8942869.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="ACHW42_000938"
                     /gene_synonym="yebK"
     CDS             complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="ACHW42_000938"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR8942870.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14729..16204
                     /gene="zwf"
                     /locus_tag="ACHW42_000939"
     CDS             14729..16204
                     /gene="zwf"
                     /locus_tag="ACHW42_000939"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR8942871.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16439..18250
                     /gene="edd"
                     /locus_tag="ACHW42_000940"
     CDS             16439..18250
                     /gene="edd"
                     /locus_tag="ACHW42_000940"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR8942872.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18287..18928
                     /gene="kdgA"
                     /locus_tag="ACHW42_000941"
                     /gene_synonym="eda"
     CDS             18287..18928
                     /gene="kdgA"
                     /locus_tag="ACHW42_000941"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR8942873.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18984..20162)
                     /gene="purT"
                     /locus_tag="ACHW42_000942"
     CDS             complement(18984..20162)
                     /gene="purT"
                     /locus_tag="ACHW42_000942"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR8942874.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20296..20586
                     /gene="yebG"
                     /locus_tag="ACHW42_000943"
                     /pseudo
     CDS             20296..20586
                     /gene="yebG"
                     /locus_tag="ACHW42_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20653..21009
                     /gene="yebF"
                     /locus_tag="ACHW42_000944"
     CDS             20653..21009
                     /gene="yebF"
                     /locus_tag="ACHW42_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR8942875.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21336..21995
                     /locus_tag="ACHW42_000945"
     CDS             21336..21995
                     /locus_tag="ACHW42_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR8942876.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22205..24265
                     /gene="ptrB"
                     /locus_tag="ACHW42_000946"
     CDS             22205..24265
                     /gene="ptrB"
                     /locus_tag="ACHW42_000946"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR8942877.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="ACHW42_000947"
     CDS             complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="ACHW42_000947"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR8942878.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24948..25604)
                     /gene="yobB"
                     /locus_tag="ACHW42_000948"
                     /pseudo
     CDS             complement(24948..25604)
                     /gene="yobB"
                     /locus_tag="ACHW42_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
     gene            complement(25706..25936)
                     /gene="holE"
                     /locus_tag="ACHW42_000949"
     CDS             complement(25706..25936)
                     /gene="holE"
                     /locus_tag="ACHW42_000949"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR8942879.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26075..26449
                     /gene="yobA"
                     /locus_tag="ACHW42_000950"
     CDS             26075..26449
                     /gene="yobA"
                     /locus_tag="ACHW42_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR8942880.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26453..27325
                     /gene="copD"
                     /locus_tag="ACHW42_000951"
                     /pseudo
     CDS             26453..27325
                     /gene="copD"
                     /locus_tag="ACHW42_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            27338..27679
                     /locus_tag="ACHW42_000952"
     CDS             27338..27679
                     /locus_tag="ACHW42_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR8942881.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28072..29149)
                     /locus_tag="ACHW42_000953"
                     /pseudo
     CDS             complement(28072..29149)
                     /locus_tag="ACHW42_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(29115..29396)
                     /locus_tag="ACHW42_000954"
     CDS             complement(29115..29396)
                     /locus_tag="ACHW42_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR8942882.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         7437 a         7235 c         7568 g         7226 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagataca aatatacgta atagcataaa aaaattattt ttcaggagtc
     4861 gcaatgtata tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt
     4921 ccaatataac ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg
     4981 caagttttct tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat
     5041 ctttgattca ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa
     5101 aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg
     5161 atgacctgtt tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt
     5221 gtggtgcgtg aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg
     5281 ttcaaagagt tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc
     5341 ggaatgtcag cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg
     5401 aaactgccgg gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga
     5461 tttaaaggtt tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct
     5521 gctagcccgg cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc
     5581 tataaaccac aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt
     5641 gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag
     5701 accgtctgat ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc
     5761 ctaaattact ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca
     5821 tcaaagcagc gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg
     5881 ggttgggtaa aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca
     5941 cgacttctgg tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg
     6001 aaccgcatga cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag
     6061 tgctgtaccc ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg
     6121 cacgctccat taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag
     6181 gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc
     6241 aggtgccgga tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga
     6301 gtgatgacgg tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc
     6361 gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag
     6421 atatcgctgc tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg
     6481 accgcaaatt gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata
     6541 acctggcggc agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt
     6601 tgattcagca aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt
     6661 ggaatcactt tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg
     6721 catccgacac tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga
     6781 gatagcgaac cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga
     6841 tagattagct ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca
     6901 ccaccgacgg acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg
     6961 ccaccatccc caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac
     7021 gcgcagtagc tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca
     7081 tcgctacacc aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt
     7141 tcacaccatc aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt
     7201 gccagaacaa aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg
     7261 gcgtcactgc cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca
     7321 tcagactaac gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg
     7381 tgtcgatcgc cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg
     7441 taacggcaat caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa
     7501 gtaatgaggc atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg
     7561 aacccagcgg acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata
     7621 attcaatcat gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg
     7681 atgattatga tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga
     7741 aataaactcc ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt
     7801 caggcacagc acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa
     7861 aacgccacaa tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc
     7921 atttacatcc acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa
     7981 tgctcgcgct aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt
     8041 aatcaggtgc ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc
     8101 agggcgtaag cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt
     8161 ctgcggtaca tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt
     8221 taccagcccg agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa
     8281 agtcaaaatt tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc
     8341 aaaagaaacc gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca
     8401 gtagtcatga aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt
     8461 atgttacata aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca
     8521 acacaggccg cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct
     8581 gccattgctg atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat
     8641 gattattcac tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg
     8701 gttggcccgg agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag
     8761 caggtaacga ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc
     8821 gatgatgatg atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc
     8881 aacatgcatc tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga
     8941 aaattagtgg aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt
     9001 gaggcacaat tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg
     9061 aaaggttatt tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca
     9121 ccgcttggtc attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa
     9181 ataagaacac agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg
     9241 ccagcggtcg ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc
     9301 ctggggacga atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc
     9361 aaccagtatg cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata
     9421 gcccgctctg tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg
     9481 tcgctcaccg ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat
     9541 gccacgccaa ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct
     9601 gaagccagtg agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa
     9661 ctggatgaca aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat
     9721 acgctaagca gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct
     9781 gctgccgaca aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc
     9841 gcggacggcg aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat
     9901 gaccgtactg ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt
     9961 aacaatctgc tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt
    10021 ttaaccagcg ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc
    10081 aaactgaaaa aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa
    10141 cgtgagcaaa gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca
    10201 atccgcgctg aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc
    10261 ttgcgattcc cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact
    10321 aatccggtga caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt
    10381 acgccagtgc tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca
    10441 ggttattatg tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc
    10501 aagattctgg tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt
    10561 aataccggac gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc
    10621 gtaaacccgc tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc
    10681 gaattcctgg cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc
    10741 attcgcagcc ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc
    10801 cgctacacta tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga
    10861 aaaaaaataa tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact
    10921 ggggagcatg gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc
    10981 gtgatcccat tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc
    11041 gtcgtgcgtt aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag
    11101 cgattgttga tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg
    11161 caatacgcgg gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg
    11221 aagagatgcg gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg
    11281 atattcctgc catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc
    11341 agggcaaccc ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc
    11401 tgcatgcgag aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg
    11461 gatattattt acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg
    11521 ccacctataa agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg
    11581 ttgtaccgct gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc
    11641 caccgatgga tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag
    11701 aagtcgagat ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga
    11761 aaactcgcaa accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat
    11821 aaaaaagcct ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa
    11881 tattcgaaat ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat
    11941 ccggcaacgt acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact
    12001 catcacgtcg ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg
    12061 cagcagatta accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagggcac
    12121 cggcgtaacg ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc
    12181 gaaaattggt agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt
    12241 catggtaatg atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc
    12301 aatagcttct tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct
    12361 cgggattttt tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata
    12421 ctgcccagcg gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt
    12481 tgctacgtcc atgacttctg cacgcgtcgg catcgggtta gtaatcattg actccatcat
    12541 ctgggtcgcc gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg
    12601 aatgccgacc agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac
    12661 cacgtcagag gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg
    12721 ttcaaccttg gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata
    12781 gttcagatct tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc
    12841 cgcagtctta atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc
    12901 aagtttgttg atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac
    12961 tttcatgccc tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat
    13021 gtcaccaggc acgacgtcag taggcaggcc tttgtagtcg ataccgactt tttctttgtc
    13081 gccttcacct ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac
    13141 tttgccttct ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc
    13201 cacatgacgc cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc
    13261 ttcaggcgag ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt
    13321 ttcaagatta ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg
    13381 aagccttttg gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga
    13441 aatccgaatg aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag
    13501 attaggacag tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa
    13561 aacgcgattc cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc
    13621 gtcgcaaagt aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa
    13681 ccatgggcat ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt
    13741 cccgggcgag cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg
    13801 ccagctgcgc cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca
    13861 cgtctccgtc gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca
    13921 ccaccggaac attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag
    13981 agcctaatcc gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga
    14041 tggcagattt atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata
    14101 ttttccccgt gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag
    14161 tgccattcgc cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca
    14221 tgctgcgaca gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag
    14281 caatactcga atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct
    14341 ctgatttgct caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa
    14401 gagactcacg ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga
    14461 tattacgcct gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca
    14521 taacatgatc agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg
    14581 ccgcgctttg cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca
    14641 agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta
    14701 ccgggttagt taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga
    14761 cctggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta
    14821 tcaactggaa aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc
    14881 tgactgggat aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa
    14941 agaaaccatt gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct
    15001 cgatgtcaat gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg
    15061 tatcactatt aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct
    15121 tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac
    15181 gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca
    15241 ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg
    15301 ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat
    15361 taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca
    15421 gatgcgtgac atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc
    15481 gccatctgac ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct
    15541 gcgccgcatc gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg
    15601 cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag
    15661 caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt
    15721 gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta
    15781 tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa
    15841 actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc
    15901 tggccttgac cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac
    15961 ctttaatcag acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg
    16021 tattcaggca ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc
    16081 cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg
    16141 gggacccgtt gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga
    16201 gtaatatcag cgcttatcct ttatggttat tttaccggta acatgatctt gcacagattg
    16261 tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta
    16321 cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc
    16381 tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat
    16441 gaatccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc
    16501 tgcttatctc gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc
    16561 atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag cctctttgaa
    16621 aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca
    16681 ccagccttat gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt
    16741 tggtcaggtt gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg
    16801 aatggaattg tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc
    16861 ccataacatg tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct
    16921 gacgatggca gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat
    16981 ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa
    17041 agtggaccgc atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg
    17101 tactttctac ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt
    17161 gccaggctct tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc
    17221 tgcgcgtcag gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat
    17281 gatcgatgag aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac
    17341 taaccacacc atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga
    17401 tgacttctct gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc
    17461 ggccgatatt aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct
    17521 caaagcaggc ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta
    17581 tacccttgaa ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc
    17641 actcgacagc aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa
    17701 agtgttaagc ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa
    17761 ccaggtgatt gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt
    17821 tgaagcgggt ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc
    17881 gaacggaatg ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg
    17941 tttcaaaatt gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc
    18001 agctatccac gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg
    18061 ggacatcatt cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga
    18121 actggctgct cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg
    18181 tgaattattc agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat
    18241 cactttttaa gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa
    18301 aacaagtgca gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa
    18361 actggaacac gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga
    18421 agtgactctg cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc
    18481 tgaagcgatt gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga
    18541 agcgggtgca cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac
    18601 cgaagggact attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat
    18661 ggactacggt ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc
    18721 cctgcaggcg atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc
    18781 tccggctaac taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg
    18841 gctggttccg gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg
    18901 tgaagctgta gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt
    18961 tttgtagcgt ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc
    19021 gcgcgttcaa tggcgtcaac aacactctct gaagtagcca gtaccacccc cagatgacgg
    19081 ctgccatcaa tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc
    19141 tgcacattat caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct
    19201 gcaggaccat actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg
    19261 gcaaactctg agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg
    19321 gagacctcac tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca
    19381 taaccgccca gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc
    19441 gggctcattt gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct
    19501 actggtgcac agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag
    19561 tcaaacttaa cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga
    19621 gcgtattccc atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg
    19681 gaagagctca tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg
    19741 aaaaggcttt tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc
    19801 gccaggcgac ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca
    19861 acattcagtc cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc
    19921 ggcacgatat aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc
    19981 atattaatga catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg
    20041 acggcaatca cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca
    20101 ccggagccta ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc
    20161 ataacgaata tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc
    20221 tttattgctg atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat
    20281 tatgagaggc tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag
    20341 aaaatgtcgt ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg
    20401 gatctgcttg acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa
    20461 gccctttcgc tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc
    20521 aaattaccgt ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg
    20581 gcctgaagca acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct
    20641 atggagaaaa acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc
    20701 gcatcagttt tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa
    20761 ggtctggatg ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg
    20821 gcgcgctggg cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt
    20881 ttgcaggaca ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa
    20941 agtgccgata ttcattacca ggtctgcgtg gactgcaaag cgggaatggc ggaatatcag
    21001 cggcgttaat ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg
    21061 tccgtcccag aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc
    21121 aatcagcatc aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat
    21181 cacctgatat acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc
    21241 cccgcttatt tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg
    21301 ctacaggcac taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc
    21361 aatccctgct tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca
    21421 aactgttagt gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag
    21481 cacgtaaact tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag
    21541 cgggtacggt gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac
    21601 cgcagtttgg cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc
    21661 tggtctttgc cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc
    21721 agcaattgcg tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg
    21781 aacaaccgct ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg
    21841 aaatctattt cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc
    21901 tgaatgcact gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg
    21961 atctcgaaca gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt
    22021 tgactatttc gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct
    22081 gcataacatt cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt
    22141 cgcttgcatc cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa
    22201 taacatgcta ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg
    22261 catcgataat tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta
    22321 cctgcaacaa gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga
    22381 tcgcatctta aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat
    22441 aaaaaatggc taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca
    22501 gcgtcaatcg gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa
    22561 gcgcgcagct catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac
    22621 cattatggcg ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa
    22681 tctggaaaca ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg
    22741 ggcaaatgac tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta
    22801 tcaggtctgg cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga
    22861 aaaagacgat acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat
    22921 tcatctggcc agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc
    22981 cgagccgttt gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca
    23041 gcatcgtttt tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg
    23101 tatgcgtgat gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga
    23161 agggtttacg ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag
    23221 tttgcgccaa attaaccgca agacccggga agtcattggc attgcctttg atgatccggc
    23281 ctatgtgacc tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta
    23341 ttcttccatg accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg
    23401 tgtattaaaa caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct
    23461 gtggatagtc gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca
    23521 ttttcgcaaa ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat
    23581 tgatgccgat ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat
    23641 tgtccatgtt cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct
    23701 gaagaagaaa aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg
    23761 ctatggctct ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg
    23821 cgttgcaatt aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt
    23881 tgatgttgta acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga
    23941 gtggggaaat ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga
    24001 caacgtcacc gcacaggctt atccgcattt actggtaacg accggtttac acgattctca
    24061 ggtgcaatat tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga
    24121 ccatctttta ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt
    24181 taaatcgtac gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac
    24241 attacccgct acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag
    24301 ctccgggctc atgctgtcca ggttattaaa taaccagcgc agatagcccg gatagcgttc
    24361 ggcaacgtcg gaaaccgctt tgccacggta tttgccaaag gtgaaggttg tcatcaacga
    24421 cggacgtccg gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc
    24481 gataagcaac gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccag
    24541 cggtgtctgt acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc
    24601 tggccacaaa cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag
    24661 tacgcggcgg tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg
    24721 gatcacatct tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc
    24781 catcgcttgt ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat
    24841 ttttccgtca atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt
    24901 ttctgtatcg ataatgcgca acatggcatg atccaggccg aaaaagccta gcgtaatgga
    24961 atgatatcgc cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga
    25021 tcggcgcgaa cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt
    25081 gccatcagca cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc
    25141 agcaggtcgg gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata
    25201 tccatacctt gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat
    25261 gcaccatgac tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt
    25321 ccacgaataa agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag
    25381 gtcgttgcgg cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca
    25441 cgtcgcgaat aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt
    25501 tgcctggcgg cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt
    25561 ttacgaggct catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca
    25621 aaatgactgc aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt
    25681 aagaaagtgt aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca
    25741 gattgaccga agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt
    25801 ctggctgttc acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag
    25861 ccaccccggc cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag
    25921 ccagattctt cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga
    25981 gcctggatgg caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc
    26041 tgtaaaaaag ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg
    26101 ctacgcgctg gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca
    26161 tttaacgcat cagtatcctg cggcaaacgc gcaagtgaca gctgccccgc aggcaatcac
    26221 tttaaacttc tcggaaggcg ttgaaacagg attcagtggt gcaaaaatca cggggccaaa
    26281 aaacgaaaat attaaaacat taccagcgaa gcggaatgag caggaccaaa agcaactgat
    26341 tgttccactg gccgattcgc tgaaacccgg aacctacacc gttgactggc atgttgtttc
    26401 ggtggacggg cataaaacga aaggacacta cacctttagc gtgaaataaa tcatgctggc
    26461 gtttacctgg atcgcgctgc gatttatcca tttcacctcg ttgatgctgg tctttggctt
    26521 tgcaatgtat ggtgcctggc tggccccctt aatgattcgt cgcttgctga cgaagcgttc
    26581 tctgcgttta caacagcacg ctgccgtctg gagtttgatc agtgccactg caatgctcgc
    26641 cgttcagggc gggttgatgg gaacgggatg gacagatgta ttttcgccaa acatttggca
    26701 ggctgttttg caaacgcagt ttggtggcgt ctggttatgg caaattgttc tcgccctcgt
    26761 aacgttgatc gtcgccctta tgcaaccgcg gaatatgcca cgtttgctgt ttatgctcac
    26821 taccgcgcaa tttattctgc tggcgggagt ggggtatgcg acgctgaatg aaggggtaac
    26881 agcgaaaatc catcagacta atcacgcgat tcacctgatt tgtgcggcgg cgtggtttgg
    26941 gggattactg ccggtgctct ggtgtatgca actcatcaaa ggccgttggc gacactaggc
    27001 tattcaggcg ctgatgcgtt tttcctggtg cgggcatttt gcggtgatcg gcgtactggc
    27061 aagcggcgtg cttaatgcat tgttgattac cggatttccc cccacgctta cgacttactg
    27121 gggccaactc ttattgctca aagccatcct ggtgatgatt atggtggtca ttgcgctggc
    27181 taatcgttat gttcttgtac cgcgcatgag gcaggatgaa gatcgtgcgg caccctggtt
    27241 tgtgtggatg actaaactcg aatgggcgat aggtgcggta gtgctggtga tcatcagcct
    27301 gcttgcgact ctcgaacctt tttgatggat tacgaaaatg atgaaaaaaa gtattctggc
    27361 gtttctgtta ctcaccagtt ctgcagcggc gctggcggct ccacaggtga taaccgtgag
    27421 tcgttttgaa gtgggtaaag acaaatgggc gtttaatcgc gaagaggtaa tgctgacttg
    27481 ccgaccgggt aatgctttgt atgtcatcaa cccaagtacc ctcgtgcagt atcctttaaa
    27541 cgatatcgca caaaaggaag ttgccagtgg gaagaccaat gcccaaccca tttcggtgat
    27601 tcagattgat gatcctaaca atcccggcga aaaaatgagt ctggcaccgt ttatagaacg
    27661 agctgaaaaa ctctgttaat tacctaaaat agccttttga tttccaataa aaaaaccgcc
    27721 tcagttcttt caccagaacg ggcggttttt aacatttaag ctgatgagca ccgcgctttt
    27781 tattgaccat tttgcacgca aactggaaaa cctggcgtcg tcatctattc ttaaagggca
    27841 aggcaactaa gcctgcatta atgccaactt ttagcgcacg gctctctccc aagagccatt
    27901 tccctggacc gaatacagga atcgtattcg gtcttttttt aattgtattt aaaatcaatt
    27961 agttgcaaac gtctccccga aattccccga aatttactcg aatttctgta ttccggtctt
    28021 ttttggttat atcacaacca aaatacattt aacaatccat ttacgttaaa atcagagcag
    28081 taagtacgtt ttttctctct catcaagata catttttgtt gtcttctccg atgtgtggcc
    28141 aagtagacgc tgagcaaatt cttctccaca tatttctttg tacaatcgtc cagccaggct
    28201 tctgatttcg tgaaaagttg gtgggttttc actgaacagt gatacctgtt aattttctgg
    28261 ctgcgacaaa tttctttgtc aggccgtccg ggtgaatgct gccgtcaggg ctgttttttc
    28321 taatcccggc actgattaga taatcccccc tacttaccat gcggcactgt tcaactactg
    28381 caccaagtcg taaaccagcg acaggaaggc tgagtgacag ggggatagca atcatcattc
    28441 ctgtcttaat ttgcctgatg tggagacgat catcataaat atcactgaac cgcatattcg
    28501 ttatgtcttc gcgacgttgt cctgttacaa gggctaagtc catagctaat gggaaccatg
    28561 ccggaagttg atctgctgcc tccctgatgc agttgtatgt ctttagtttc agccgttctc
    28621 ttgtaactac tattttcggt gctcttgttg gcgttactgg attttgagat atacgtcctt
    28681 caacaatggc ttcgcggaac atatcagata acacagaacg cattgatcct gccatcgtgt
    28741 ttttccctcc ttcaatctac aaatcaagaa actcggcgat atggcgagtg gttatttctg
    28801 tcagtaaaac ccctcctaat ttttctttta ttgtctccag ttgatttacc cgagttttat
    28861 aagtatttct ggacgctttt ctccttataa gaatcgtttt gtaacgttca atccagcctg
    28921 ccatagtaaa tgagtcgacc cctttaagct tttcaattag ggcagcagga gagtagtttt
    28981 tgtatatata atgatttgct tcaattgcct gcgctactgc atctcttctt gaaattttac
    29041 ctagtgtaaa ttcttctttc gtcagagggt tgcgccagta atatgctttg tctctccttc
    29101 gatatgttaa gtttctaggc aaattgggat cgtatttttt ccgctgcatg ttttaacttc
    29161 tccattaacg gactgtctct cccttgtcgc ccattaggct gatggtgtgt tatatcggta
    29221 tcaaccttat ttgggttgat atagaaagcc tccggaacca ccctgtaact cctcccgtgt
    29281 agttcaggtg caggataaat gtttccattc cttgcccatc gtctcagcgt tgatattgat
    29341 ggtgggttat ccggatatct gagttttccc cacgttttga gtgtcacaag attcattgcc
    29401 atacctctca cgatatgacc gccagtaaat atacagaata ctggcgggtg tggttgattt
    29461 ttaata
//
LOCUS       DAZSNM010000024        29094 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZSNM010000024 DAZSNM010000000
VERSION     DAZSNM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29094
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.024"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..155
                     /locus_tag="ACHW42_000955"
     CDS             <1..155
                     /locus_tag="ACHW42_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8942883.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="ACHW42_000956"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="ACHW42_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR8942884.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="ACHW42_000957"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="ACHW42_000957"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR8942885.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="ACHW42_000958"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="ACHW42_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="ACHW42_000959"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="ACHW42_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR8942886.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="ACHW42_000960"
     CDS             complement(5626..7134)
                     /locus_tag="ACHW42_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR8942887.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACHW42_000961"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACHW42_000961"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR8942888.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="ACHW42_000962"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="ACHW42_000962"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR8942889.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="ACHW42_000963"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="ACHW42_000963"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR8942890.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACHW42_000964"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACHW42_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR8942891.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACHW42_000965"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACHW42_000965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR8942892.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="ACHW42_000966"
     CDS             complement(14283..15602)
                     /locus_tag="ACHW42_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFR8942893.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACHW42_000967"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACHW42_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR8942894.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="ACHW42_000968"
     CDS             16699..17034
                     /locus_tag="ACHW42_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR8942895.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACHW42_000969"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACHW42_000969"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR8942896.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="ACHW42_000970"
     CDS             complement(17790..19172)
                     /locus_tag="ACHW42_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8942897.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACHW42_000971"
     CDS             complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACHW42_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR8942898.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="ACHW42_000972"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="ACHW42_000972"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR8942899.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="ACHW42_000973"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="ACHW42_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR8942900.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACHW42_000974"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACHW42_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR8942901.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACHW42_000975"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACHW42_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR8942902.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="ACHW42_000976"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="ACHW42_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR8942903.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="ACHW42_000977"
     CDS             complement(25947..26768)
                     /locus_tag="ACHW42_000977"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR8942904.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="ACHW42_000978"
     CDS             complement(26868..26951)
                     /locus_tag="ACHW42_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8942905.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACHW42_000979"
     CDS             complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACHW42_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR8942906.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQPAKPAAQPAA
                     "
     gene            27623..27727
                     /locus_tag="ACHW42_000980"
     CDS             27623..27727
                     /locus_tag="ACHW42_000980"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR8942907.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(27776..29029)
                     /locus_tag="ACHW42_000981"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="ACHW42_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         7468 a         6927 c         7424 g         7275 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgtt
//
LOCUS       DAZSNM010000025        28126 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZSNM010000025 DAZSNM010000000
VERSION     DAZSNM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28126
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.025"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..985)
                     /gene="metL"
                     /locus_tag="ACHW42_000982"
     CDS             complement(<1..985)
                     /gene="metL"
                     /locus_tag="ACHW42_000982"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR8942908.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQ"
     gene            complement(988..2148)
                     /gene="metB"
                     /locus_tag="ACHW42_000983"
     CDS             complement(988..2148)
                     /gene="metB"
                     /locus_tag="ACHW42_000983"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR8942909.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            2425..2742
                     /gene="metJ"
                     /locus_tag="ACHW42_000984"
     CDS             2425..2742
                     /gene="metJ"
                     /locus_tag="ACHW42_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR8942910.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2926..3534
                     /locus_tag="ACHW42_000985"
     CDS             2926..3534
                     /locus_tag="ACHW42_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR8942911.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(3595..3807)
                     /gene="rpmE"
                     /locus_tag="ACHW42_000986"
     CDS             complement(3595..3807)
                     /gene="rpmE"
                     /locus_tag="ACHW42_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR8942912.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            4010..6208
                     /gene="priA"
                     /locus_tag="ACHW42_000987"
     CDS             4010..6208
                     /gene="priA"
                     /locus_tag="ACHW42_000987"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR8942913.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            6364..7389
                     /gene="cytR"
                     /locus_tag="ACHW42_000988"
     CDS             6364..7389
                     /gene="cytR"
                     /locus_tag="ACHW42_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR8942914.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            7481..8440
                     /gene="ftsN"
                     /locus_tag="ACHW42_000989"
     CDS             7481..8440
                     /gene="ftsN"
                     /locus_tag="ACHW42_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR8942915.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            8533..9063
                     /gene="hslV"
                     /locus_tag="ACHW42_000990"
     CDS             8533..9063
                     /gene="hslV"
                     /locus_tag="ACHW42_000990"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR8942916.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            9073..10404
                     /gene="hslU"
                     /locus_tag="ACHW42_000991"
     CDS             9073..10404
                     /gene="hslU"
                     /locus_tag="ACHW42_000991"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR8942917.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            10471..11397
                     /gene="menA"
                     /locus_tag="ACHW42_000992"
     CDS             10471..11397
                     /gene="menA"
                     /locus_tag="ACHW42_000992"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR8942918.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            11490..11975
                     /gene="rraA"
                     /locus_tag="ACHW42_000993"
     CDS             11490..11975
                     /gene="rraA"
                     /locus_tag="ACHW42_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR8942919.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(12060..12305)
                     /gene="zapB"
                     /locus_tag="ACHW42_000994"
     CDS             complement(12060..12305)
                     /gene="zapB"
                     /locus_tag="ACHW42_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR8942920.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            12731..13576
                     /gene="glpF"
                     /locus_tag="ACHW42_000995"
                     /pseudo
     CDS             12731..13576
                     /gene="glpF"
                     /locus_tag="ACHW42_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            13616..15106
                     /gene="glpK"
                     /locus_tag="ACHW42_000996"
     CDS             13616..15106
                     /gene="glpK"
                     /locus_tag="ACHW42_000996"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR8942921.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            15336..16346
                     /gene="glpX"
                     /locus_tag="ACHW42_000997"
     CDS             15336..16346
                     /gene="glpX"
                     /locus_tag="ACHW42_000997"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR8942922.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            16443..17189
                     /gene="fpr"
                     /locus_tag="ACHW42_000998"
     CDS             16443..17189
                     /gene="fpr"
                     /locus_tag="ACHW42_000998"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR8942923.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(17194..17622)
                     /gene="uspD"
                     /locus_tag="ACHW42_000999"
     CDS             complement(17194..17622)
                     /gene="uspD"
                     /locus_tag="ACHW42_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR8942924.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(17649..17948)
                     /locus_tag="ACHW42_001000"
     CDS             complement(17649..17948)
                     /locus_tag="ACHW42_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR8942925.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(18160..18600)
                     /locus_tag="ACHW42_001001"
                     /pseudo
     CDS             complement(18160..18600)
                     /locus_tag="ACHW42_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            18701..19300
                     /locus_tag="ACHW42_001002"
     CDS             18701..19300
                     /locus_tag="ACHW42_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR8942926.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            19408..20175
                     /gene="tpiA"
                     /locus_tag="ACHW42_001003"
     CDS             19408..20175
                     /gene="tpiA"
                     /locus_tag="ACHW42_001003"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR8942927.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(20230..20985)
                     /gene="cdh"
                     /locus_tag="ACHW42_001004"
     CDS             complement(20230..20985)
                     /gene="cdh"
                     /locus_tag="ACHW42_001004"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR8942928.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(21092..22081)
                     /gene="sbp"
                     /locus_tag="ACHW42_001005"
     CDS             complement(21092..22081)
                     /gene="sbp"
                     /locus_tag="ACHW42_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR8942929.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(22401..23363)
                     /gene="pfkA"
                     /locus_tag="ACHW42_001006"
     CDS             complement(22401..23363)
                     /gene="pfkA"
                     /locus_tag="ACHW42_001006"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR8942930.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(23544..24446)
                     /gene="fieF"
                     /locus_tag="ACHW42_001007"
     CDS             complement(23544..24446)
                     /gene="fieF"
                     /locus_tag="ACHW42_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR8942931.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(24594..25094)
                     /gene="cpxP"
                     /locus_tag="ACHW42_001008"
     CDS             complement(24594..25094)
                     /gene="cpxP"
                     /locus_tag="ACHW42_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR8942932.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            25244..25942
                     /gene="cpxR"
                     /locus_tag="ACHW42_001009"
     CDS             25244..25942
                     /gene="cpxR"
                     /locus_tag="ACHW42_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR8942933.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            25939..27312
                     /gene="cpxA"
                     /locus_tag="ACHW42_001010"
     CDS             25939..27312
                     /gene="cpxA"
                     /locus_tag="ACHW42_001010"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR8942934.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(27418..28092)
                     /gene="yiiM"
                     /locus_tag="ACHW42_001011"
     CDS             complement(27418..28092)
                     /gene="yiiM"
                     /locus_tag="ACHW42_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR8942935.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT         6919 a         7385 c         7256 g         6566 t
ORIGIN      
        1 cctgactggc gggcacctga aactcaatca aacagacatc atcgtggctg gtgacaatac
       61 gcgcaccagt accggaggcc agcacgcgtt caatgcgcgt ggaaccttga tccggcgtgt
      121 agctacagcg cagctgcagg tcgatttcgc tgccagaaac tggctgtaaa gtacgggcgt
      181 gaagaacggg agccgccaga cgcgccagtt cgctggcctc atccagacgc agcaacggca
      241 gcaggcaggc atctttcact ttacgcgggt cggcactgta taccccggcg acgtcgctcc
      301 agatggttac acgagaaaca cccgccagcg caccgatttg tgtcgcggaa tagtcggaac
      361 cgttacgccc cagcaacacc gtttcaccgg cgttgttacg gctgataaac ccggtcacca
      421 ccagacgttt gctcggatgt tgtaccagta gctgttgcag caacgggtaa gaaagccctt
      481 catcaacctg cggttgcgca gcgcgttcag cgcgtaaaaa ctcacgggca tcaagccagg
      541 ccgctggcag cccttgttga ttaagtaccg cagacatcag gcgcgccgac cacacttcac
      601 cgtggcccac cacttccgca tacaccgcat cgttaatacc actgtcgagc aacgccgcca
      661 ggcgctcaag atcgctgaca aaagcgctaa tgaggctatc ggcttcttca gcgggtagca
      721 gaccgctaat cagatcgcac tgatagcgac gcagcgtttg ttgaacctga tgcgcagaga
      781 gacgatcggt ctggcttagt ttcaaccagt taatcaactg gttagtggtg ctaccggcgg
      841 cggaaaccac catcatatcg tcaggctgag agaactccgc cataatgccc gcgacacgca
      901 aataacactt cacatcagcc agactactgc caccaaattt atgcagctga cgacctttcg
      961 cccctgcctg cgcaatcaca ctcattttta ccccttgttt gcagcccgga agccattttc
     1021 caggtcggca attaaatctt cgccatcttc aataccggtg gagatacgca gcagcgtctc
     1081 ggagatcccg gcggcagcac gcgcttctgg tgccatgcct gcatgtgtca tggttgcggc
     1141 gtgagagatt aaactttcca ctccccctaa tgattccgcc agcgtaaaca acgacagccc
     1201 gcccaggaaa cgacacagcg tctgctcatc gccatccagt tcaaaactca acattgcgcc
     1261 aaagcctttt tgctgacgcg cggcaatttc atgcccctga ttttccggca gcgacgggtg
     1321 atacagtttt ttcaccaacg gctgggtttg cagatatttc acaatcgcct gcgcgttgcg
     1381 ctgcgccagc tccatacgcg gcaccagcgt tcgcaacccg cgtagcagca gatagctgtc
     1441 aaacgcgccg cccgtcacgc caatattgtt tgcccaccag gcaagttcag tgacaacgtc
     1501 cgggtcttta gcaatcacca cgccagccac cacgtcagag tgaccgttca gatatttcgt
     1561 acatgaatgc aacaccagat cggcacccaa tgccagcgga ttttgtaatg ccgggcttaa
     1621 gaaggtgtta tccaccacgc tcaccgcccc gacttccctt gccagatggc agattttcgc
     1681 aatatccacg acgcgtaaca atggattact tgggctttct accagtacca gtttgggttt
     1741 ttctgccagc gctgcccgta atgcctgttc atcgccttga tcaacaaaca acacgcgata
     1801 gcaaccgcgt ttcgccagac tgtcgaacag gcgatagcta ccgccgtagc agtcgtgcgg
     1861 cgcaaccagc agatcgccag gtttcaaaaa gacggtcgtt accaggtgaa tcgcggacat
     1921 gccggtatta gtaagtactg cgccagcacc gccttccagt tctgccagcg cacgctgaac
     1981 cacatcgcgc gttgggttgc cgcgacgcga gtaatcatgc gcgcgcggtt cattaaatcc
     2041 ggtaaagtta taggtgctgg aaagatggat gggagggaca acgcaaccat actgttcgtc
     2101 gtcatttaac ccgctacgca ctgcgatggt ggcctgttta cgcgtcatgt gatgaagttt
     2161 cctgggcttt gtcggtgaaa tgtcaggcac cagagtaaac attgtgttaa tggacgtcaa
     2221 tacatctgga catctaaact tctttgcgta tagattgagc aaatcccaaa tagccgttaa
     2281 aattatatgc attatcacgc cgacaggtgc attacacgat gtcacggtaa cgcctgtacg
     2341 gtaaactatg cgggtttacg gtcagtaccc acataaattg tggggtctgg tctcaattta
     2401 ttgacgaaga ggattaagta tctcatggct gaatggagcg gcgaatatat cagcccatac
     2461 gctgagcacg gcaagaagag tgaacaagtc aaaaagatta cggtttccat tcctcttaag
     2521 gtgttaaaaa tcctcaccga tgaacgcacg cgtcgtcagg tgaacaacct gcgtcacgct
     2581 accaacagcg agctgctgtg cgaagcgttt ctgcatgcct ttaccgggca acctttgccg
     2641 gatgatgccg atctgcgtaa agagcgcagc gacgaaatcc cggaagcggc aaaagagatc
     2701 atgcgtgaga tggggattaa cccggagacg tgggaatact aaagcggaaa agagcggcgc
     2761 ggagtggaat cgcctgatgc actacgctta tcaggcctac gtcatattgc aatttattga
     2821 atttgcacga acttgtaggc cggataaggc gttcacgccg catccggcat aaacaacgag
     2881 cacgttgtct gcgacccacc gctttttata catggacgtt taactatgaa aaacaggctg
     2941 ctgatcctca gcctgctggt ttctgtacct gcctttgcct ggcagccgca aactggcgac
     3001 atcatctttc aaatctctcg ctcatcgcaa agtaaagcga tccaactggc aacccattcc
     3061 gattacagcc acaccggtat gctggtgatg cgtaacaaaa agccctacgt ttttgaagcg
     3121 gtcggcccgg tgaaatacac cccgctcaag cagtggattg cccatggtga aaagggcaac
     3181 tacgttgttc gccgcgttga aggcggactg agtgttgaac aacagcaaaa actgacgcaa
     3241 acggcaaaac gttatctcgg taaaccgtat gatttcagct tctcatggag cgacgatcgc
     3301 cagtactgtt cggaagtggt gtggaaggtt taccagaacg cgctgggaat gcgcgtgggc
     3361 gaacagcaga agttgaaaga gtttgatctc agcaacccgc tggtccaggc gaagctcaaa
     3421 gaacgctacg gtaaaaatat tccgcttgag gaaacggtag tttcaccaca agccgttttc
     3481 gacgcgccac aactcaccac ggttgccaaa gagtggccgc tgttttcgtg gtgatacgac
     3541 caaaattcag gcacaaaaaa agcgccgtgc ggcgcttttt tcggaaatcc ggtcttattt
     3601 gctgcccggg atgttgaaac gcttgttgaa gcggtcaaca cggccaccgg tagcaacatc
     3661 acgctgtttg ccagtgaaga acgggtggca cttgctgcac acgtcgaggt tcaggtcatg
     3721 accaacggtg gagcggattt ttattacgtt accgcaagag cagctagcag taatttcttc
     3781 gtatttcggg tgaatatctt ttttcatggg aaaacctcgg tttaaggccg cgtcgctctt
     3841 ccagccctaa cgccagacac cacgcgatgt taaaagtata gcttcaatac gatcatttcg
     3901 tacgaaggcg cgaaatcata cagaaattaa ccagcgtatg caaactgatc cgcactcttc
     3961 tacggcaatg tgtatactaa cccaccgaat ttcaagtcag gatgatgcta tgcccgttgc
     4021 ccacgttgcc ttgcccgttc cgcttcctcg tacctttgac tatctgctgc cagaaggcat
     4081 gacggttaaa gctgggtgtc gcgtgcgcgt gccgtttggc aaacagcagg agcgcatcgg
     4141 gattgtggta tcagttagcg atgccagcga actgccgctc actgagctaa aagcggtagt
     4201 cgaagtgctg gatggtgagc cggtgtttac tcactccgtc tggcgattgc tgctatgggc
     4261 ggcagattac tatcatcatc cgattggcga tgtgctgttt catgccttgc cgattttact
     4321 acgccagggg cggcctgcgg cgaacgcgcc gatgtggtac tggtttgcca ctgaacaagg
     4381 tcaggcggtg gatctgaaca gcctgaaacg ctcacccaag caacaacagg cgctggcagc
     4441 gttacggcaa ggcaaaatct ggcgcgacca ggttgccacg ctcgaattta atgatgccgc
     4501 gttgcaggcg ctgcgcaaaa aaggtctgtg tgatttagca agtgaaacac cagagtttag
     4561 cgactggcga acgaactatg ccgtttctgg tgagcggttg cgattgaata ccgaacaggc
     4621 caccgccgtt ggcgcaattc atagcgcggc agatactttt tctgcctggc tgctggcggg
     4681 cgttaccggt tccggtaaaa cggaggttta tctcagtgta ctggaaaacg tgctcgctca
     4741 gggcaaacag gcgctggtga tggtgccgga aatcggcctg acaccgcaaa ctatcgcccg
     4801 ttttcgtgaa cggtttaatg cgcccgtgga agttctgcat tccggtctga acgacagcga
     4861 gcgtctttcg gcgtggctga aagcgaaaaa tggcgaggcg gcgattgtga tcggcacccg
     4921 ctccgcgctg tttacgccgt ttaaaaatct cggcgtgatt gtcattgacg aagagcacga
     4981 cagttcctac aagcagcagg aaggctggcg ctatcatgcc cgcgacctgg cggtgtatcg
     5041 tgcgcatagc gagcaaatcc cgattattct tggctccgca acgcccgcgc tggaaacgtt
     5101 gtgcaacgtc cagcagaaaa aataccgcct gctgcgcctg acgcgccggg cggggaatgc
     5161 gcgtccggca attcaacacg tgctggattt aaaaggtcag aaggtgcagg caggtctggc
     5221 tccggcgtta atcacccgta tgcgccagca tttacaggcc aacaatcagg ttatcctctt
     5281 tcttaaccgc cgtggctttg cgcctgcgct actgtgccac gactgcggct ggattgccga
     5341 atgcccacgt tgcgatcact actacacgct gcatcaggcg caacagcatt tgcgctgtca
     5401 ccactgtgac agtcagcgtc cggtgccgcg ccagtgccct tcctgcggtt ccacgcacct
     5461 ggtacccgtg gggctgggca ccgaacagct tgaacagacg cttgcgccgc tgttccctga
     5521 tgtgcccatt tctcgtatcg accgcgatac caccagccgc aaaggggcgc tggaacagca
     5581 actggcggaa gtacatcgcg gcggtgcgag gattttgatc ggcacacaaa tgctggcgaa
     5641 aggtcaccac tttccggatg tgacgctggt ggcgttactg gatgtggacg gcgcgctgtt
     5701 ttctgccgat tttcgctcgg cagagcgttt cgctcagctt tacacccagg tcgctggtcg
     5761 tgccgggcgt gcgggtaaac aaggcgaagt ggtgctgcaa acgcaccatc cggaacatcc
     5821 tctgttgcaa acgttgctct ataaaggcta cgatgccttt gccgaacagg cgctggctga
     5881 gcgacggatg atgcaactgc cgccatggac cagccatgtg attgtgcgtg cggaagatca
     5941 taacaatcag catgcgccgc tgttcctgca acaactgcgt aatctgatcc tctccagccc
     6001 gctggcagac gacaaactgt gggttctcgg tccggttccg gcactggcac ctaaacgtgg
     6061 cggtcgctgg cgctggcaga tattgttgca gcacccttcc cgcgtgcgct tgcaacacat
     6121 cattagtggt acgctggcgc tcatcaatac aataccggat tcccgcaagg tgaaatgggt
     6181 actggatgtt gatccgattg agggttaaac cgcttacgat gcgaggcgga tcgaaaaatt
     6241 caatattcat cacacttttc atgaaaattc tgtaaccgtt ttcacgcgct atctgctaaa
     6301 aatgttgccg atgtgaagta aacatggatg tagtacgcct gacgtgccag gcgaggagtg
     6361 agtgtgaaag cgaagaagca ggaaactgcc gcgaccatga aagacgttgc cctcaaggca
     6421 aaagtctcta cagcgactgt ctcccgagca ttaatgaatc ccgataaagt ctcccaggcc
     6481 acccgtaatc gggttgaaaa agcggcccgg gaagtgggtt atttaccgca gcctatgggg
     6541 cgcaacgtca agcgtaatga atcccgcacc attctggtga ttgtcccgga tatctgcgat
     6601 cccttcttta gcgaaattat tcgcggtatc gaagttacgg cggcaaatca cggatatctg
     6661 gtgctgattg gcgactgtgc gcatcaaaat cagcaggaaa aaacctttat cgatttgatc
     6721 atcaccaagc aaattgatgg catgttgctg ctgggttcaa ggctgccgtt tgatgccagc
     6781 attgaggaac agcgtaatct gccgccgatg gtgatggcga acgaatttgc accggaactg
     6841 gagctgccta ccgttcatat cgacaatctg accgccgcat ttgatgcagt aaattattta
     6901 tatgagcaag ggcataaacg gattggctgt atagccggtc ccgaagagat gccactgtgt
     6961 cactaccgcc tgcaaggcta tgttcaggcg ctgcgtcgct gcggaattat ggttgatcca
     7021 caatacatcg cccgtggcga cttcaccttc gaagccggaa gcaaagcaat gcaacagctg
     7081 cttgatcttc cacaaccgcc tactgcagtc ttctgccata gcgatgtgat ggcgctcggc
     7141 gcactttctc aggcaaaacg ccaggggctg aaagtcccgg aagacctttc cataatcggt
     7201 tttgataaca tcgacctgac gcaattttgt gatccgccgc tgacaaccat cgcgcagccg
     7261 cgttacgaaa tcggtcggga agctatgctg ttattgcttg atcaaatgca ggggcaacac
     7321 gttggcagtg gctctcgttt aatggactgc gaacttatca tccggggatc aacacgcgcg
     7381 ttaccttaaa gtaaaccttt aagactgccg tgactggtca aaggcccgcc gcttaagtaa
     7441 catggcgggc tgacgaacga ataaatacag cgaaacgata gtggcacaac gagattatgt
     7501 acgccgcagc caaccggcac cttcgcggcg aaaaaagagc acctcacgga aaaagcaacg
     7561 aaatctgcct gcggtttctc ccgctatggt cgctattgct gccgccgttc ttgtgacctt
     7621 tatcggtggt ctgtacttca ttacgcatca caagaaagaa gagtccgaga cgctgcaaag
     7681 ccagaaagtg accggaaacg gactaccacc aaaaccagaa gaacgctggc gctacattaa
     7741 agagctggaa agtcgccagc cgggtgtgcg tgcgcccaca gaaccttctg ccggtggtga
     7801 agtgaaaacg ccggagcaac tgacaccaga acaacgtcag cttcttgaac aaatgcaggc
     7861 tgatatgcgc cagcagccaa cgcagctggt tgaggttccg tggaatgaac agacgccgga
     7921 acagcgtcag caaacgctac aacgccagcg tcaggcgcag caattagcgg agcagcaacg
     7981 cctggctcag cagtcgcgta cgactgaaca aagctggcag cagcagacgc gtacgtcgca
     8041 agccgcgccg gtacaggcgc aaccgcgcca gtctaaacca gcttctactc agcagccgta
     8101 ccaggatctg ctgcaaaccc ctgcgcacac gaccgcgcag tcgaagccgc agcaagctgc
     8161 gccggttgct cgtgccgctg acgcgccaaa accgacggcg gagaaaaaag acgaacgccg
     8221 ctggatggtg cagtgcggtt cgttcagagg cgcggaacag gcagagacgg tacgtgctca
     8281 gctggcgttc gaaggatttg actcgaaaat caccaccaac aatggctgga atcgtgtggt
     8341 cattggcccg gtgaaaggca aagagaacgc agacagcacc ctcaatcggt tgaagatggc
     8401 gggtcataca aactgcattc ggctcgccgc cgggggttga aaccctcaaa atccccccca
     8461 tctataattg cattatgccc cgtacttttg tacggggttt gtactctgta ttcgtaacca
     8521 aggggtcagc tcgtgacaac tatagtaagc gtacgccgta acggccatgt ggtcatcgct
     8581 ggtgatggtc aggccacgtt gggcaatacc gtaatgaaag gcaacgtgaa aaaggtccgc
     8641 cgtctgtaca acgacaaagt catcgcgggc tttgcgggcg gtactgcgga tgcttttacg
     8701 ctgttcgaac tgtttgaacg taaactggaa atgcatcagg gccatctggt taaagccgcc
     8761 gttgagctgg caaaagactg gcgtaccgat cgcatgctgc gcaaacttga agcactgctg
     8821 gcagtcgcgg atgaaactgc atcgcttatc atcaccggta acggtgacgt ggtgcagcca
     8881 gaaaacgatc ttattgctat cggctccggc ggcccttacg cccaggctgc ggcgcgcgcg
     8941 ctgttagaaa acactgaact tagcgcccgt gaaattgctg aaaaggcgtt ggatattgca
     9001 ggcgacattt gcatctatac caaccatttc cacaccatcg aagaattaag ctacaaagcg
     9061 taaggatctc ccatgtctga aatgacccca cgcgaaatcg tcagcgaact ggataagcac
     9121 atcatcggcc aggacaacgc caagcgttct gtggcgattg ctctgcgtaa ccgctggcgt
     9181 cgcatgcagc tcaacgaaga gctgcgccat gaagtgaccc cgaaaaatat cctgatgatc
     9241 ggcccgaccg gtgtcggtaa aactgaaatc gcccgtcgtc tggctaagct ggcgaatgcg
     9301 ccgttcatca aagttgaagc gaccaaattt accgaagtgg gctacgtcgg taaggaagtt
     9361 gattctatta ttcgcgatct gaccgatgcc gccgtgaaaa tggtacgcgt ccaggctatc
     9421 gagaaaaacc gttatcgcgc tgaagaactg gcagaagaac gtattctcga cgtgctgatc
     9481 ccacctgcta aaaacaactg gggacagact gaacagcagc aggaaccttc cgctgctcgt
     9541 caggcattcc gcaaaaaact gcgtgaaggc cagcttgatg acaaagaaat cgagatcgat
     9601 cttgccgcag caccgatggg cgttgaaatt atggctcctc cgggcatgga agagatgacc
     9661 agccagctgc agtccatgtt ccagaacctg ggcggccaga agcaaaaagc gcgtaagctg
     9721 aaaatcaaag acgccatgaa gctgctgatt gaagaagaag cggcgaaact ggtgaacccg
     9781 gaagagctga agcaagacgc tatcgacgct gttgagcagc acgggatcgt gtttatcgac
     9841 gaaatcgaca aaatctgtaa gcgcggcgag tcttccggtc cggatgtttc tcgtgaaggc
     9901 gttcagcgtg acctgctgcc gctggtagaa ggttgcaccg tttccaccaa acacgggatg
     9961 gtcaaaactg accacattct gtttatcgct tctggcgcgt tccagattgc gaaaccgtct
    10021 gacctgatcc cggaactgca aggtcggctg ccaatccgcg ttgaactgca ggcactgacc
    10081 accagcgact tcgagcgtat tctgaccgag ccgaatgcct ctatcaccgt gcagtacaaa
    10141 gcactgatgg cgactgaagg cgtaaatatc gagtttaccg actccggtat taaacgcatc
    10201 gcggaagcgg catggcaggt gaacgaatct accgaaaaca tcggtgctcg tcgtttacac
    10261 actgttctgg agcgtttaat ggaagagatt tcctacgacg ccagcgattt aagcggtcaa
    10321 aatatcacta ttgacgcaga ttatgtgagc aaacatctgg atgcgttggt ggcagatgaa
    10381 gatctgagcc gttttatcct ataatcgcgt tcaatcattt tcatcattgt ttgatggggc
    10441 tgaaaggccc catttttatt ggcgcgtatt atgactgaac aacaaattag ccgaactcag
    10501 gcgtggctgg aaagtttacg acctaaaacc ctccccctcg cctttgctgc aattatcgtc
    10561 gggacggcgc tggcatggtg gcaaggtcac ttcgatccgc tggtcgccct gctggcacta
    10621 attaccgccg ggctattaca gatcctttct aacctcgcca atgattacgg cgatgcggta
    10681 aaaggcagcg ataaacctga ccgcattggg ccgctacgcg gcatgcaaaa aggggtcatt
    10741 acccagcaaa agatgaaacg ggcgctcatt attaccgtcg tgctcatctg tctctccggg
    10801 ctggcactgg ttgcagtggc atgccatacg ctggccgatt ttgtcggttt cctgattctt
    10861 ggcgggttgt cgatcattgc cgctatcacc tacaccgtgg gcaatcgtcc ttatggttat
    10921 atcggtctgg gtgatatttc cgtactggtt ttctttggct ggttgagtgt catggggagc
    10981 tggtatttac aggctcatac attgattccg gcactgatcc ttccggcgac cgcatgcggc
    11041 ctgctggcaa cggcagtact gaatattaat aacctgcgtg atatcaatag cgaccgcgaa
    11101 aatggcaaaa acacgctggt ggtgcgctta ggtgaagtga acgcgcgtcg ttatcatgcc
    11161 tgcctgctga tgggctcgct ggtgtgtctg gcgctgttta atctcttttc gctgcatagc
    11221 ctgtggggct ggctgttcct gctggcggca ccattactgg tgaagcaagc ccgttatgtg
    11281 atgcgggaaa tggacccggt ggcgatgcga ccaatgctgg aacgtactgt caagggagcg
    11341 ttactgacta acctgctgtt tgttttaggg atattcctaa gccagtgggc agcataactg
    11401 acaaatatca attaacaatt gatgattttg ccaacagccc acatagcgcg atatactgaa
    11461 aattctcgca gcaactgaat gttaagccta tgaaatacga tacttccgag ctttgtgaca
    11521 tctatcaaga agatgttaac gtcgtggaac cgctgttctc caactttggc ggacgggcgt
    11581 cgtttggcgg acaaataatc acggtaaaat gtttcgagga caacgggttg ctgtacgatc
    11641 tgctcgaaca gaatggccgt ggtcgtgttc ttgtcgtcga tggcggtggt tctgttcgtc
    11701 gcgcactggt cgatgctgaa ctggcgcgtc tggcagtaca aaatgaatgg gaaggtctgg
    11761 tcatttacgg cgcggtgcgt caggtagatg acctggaaga gttggatatc ggcatccagg
    11821 cgatggcggc aattccggtt ggtgccgctg gcgaaggcat tggcgaaagc gatgtccgcg
    11881 tcaattttgg cggtgtcacc ttcttctccg gcgaccatct ttatgccgac aataccggga
    11941 ttattctttc agaagatccg ctggatattg aatgataaga aaggcaccgc aaggtgcctt
    12001 ttttctgcgt tacctgttgg cctacacagt aaagaaatta cgcgaaagat gaagcgtaat
    12061 cagacctctt ccatgcgacc cagcagggcc tgcagacgtt cctgccagcc gttctgctgt
    12121 tctttcagat ggttgttctc acgctccagc tcttcgcgct gatgctgggc attttgaact
    12181 tcctgcgaca gtgagttgtt tttttctttc agctcttcga tttccatctg caacagagtg
    12241 atggtatcaa tcgcctgctg tacttttgct tccagtttct caaacacttc taatgacatt
    12301 gtcatacctc tcctgaattg caaggcgttg atggataaaa aatcctcgtc ccgattaccg
    12361 gtgacgcctt aataaatacg agcgcacttt agttagctcc gattgtatga agccgcgcca
    12421 tcgctgtcca gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctccta
    12481 gtgacataat ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa
    12541 acatgcatca tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgaa
    12601 gcacacacat tttaagttcg atatttctcg tttttgctcg ttaacgataa gttaacagca
    12661 tgcctacaag catcgtggag gtccgtgact ttcacgcata caacaaacat taactcttca
    12721 ggatccgatt atgagtcaaa catcaacctt gaaaggccag tgcattgctg aattcctcgg
    12781 taccgggttg ttgatttttt tcggtgtggg ttgcgttgca gcactaaaag tcgctggtgc
    12841 gtcttttggt cagtgggaaa tcagtgtcat ttggggactg ggggtggcaa tggccatcta
    12901 cctgaccgca ggggtttccg gcgcgcatct taatcccgct gttaccattg cattgtggct
    12961 gtttgcctgt ttcgacaagc gcaaagttat tccttttatc gtttcacaag ttgccggcgc
    13021 tttctgcgct gcggctttag tttacgggct ttactacaat ttatttttcg acttcgagca
    13081 gactcatcac attgttcgcg gcagcgttga aagtgttgat ctggctggca ctttctctac
    13141 ttaccctaat cctcatatca attttgtgca ggctttcgca gttgagatgg tgattaccgc
    13201 tattctgatg gggctgatcc tggcgttaac ggacgatggc aacggtgtac cacgcggccc
    13261 tttggctccc ttgctgattg gtctactgat tgcggtcatt ggcgcatcta tgggcccatt
    13321 gacaggtttt gccatgaacc cagcgcgtga cttcggtccg aaagtctttg cctagctggc
    13381 gggctggggc aatgtcgcct ttaccggcgg cagagacatt ccttacttcc tggtgccgct
    13441 tttcagccct atcgttggcg cgattgtagg tgcatttgcc taccgcaaac tgattggtcg
    13501 ccatttgcct tgcgatatct gtgttgtgga agaaaaggaa accacaactc cttcagaaca
    13561 aaaagcttcg ctgtaatatg actacgggac aattaaacat gactgaaaaa aatatatcgt
    13621 tgcgctcgac cagggcacca ccagctcccg cgcggtcgta atggatcacg atgccaatat
    13681 cattagcgtg tcgcagcgcg aatttgagca aatctaccca aaaccaggtt gggtagaaca
    13741 cgacccaatg gaaatctggg ccacccaaag ctccacgctg gtagaagtgc tggcgaaagc
    13801 cgatatcagt tccgatcaaa ttgcagctat cggtattacg aaccagcgtg aaaccactat
    13861 tgtctgggaa aaagaaaccg gcaagcctat ctataacgcc attgtctggc agtgccgtcg
    13921 taccgcagaa atctgcgagc atttaaaacg tgacggttta gaagattata tccgcagcaa
    13981 taccggtctg gtgattgacc cgtacttttc tggcaccaaa gtgaagtgga tcctcgacca
    14041 tgtggaaggc tctcacgagc gtgcacgtcg tggtgaattg ctgtttggta cggttgatac
    14101 gtggcttatc tggaaaatga ctcagggccg tgtccatgtg accgattaca ccaacgcctc
    14161 tcgtaccatg ttgttcaaca tccataccct ggactgggac gacaaaatgc tggaagtgct
    14221 ggatattccg cgcgagatgc tgccagaagt gcgtcgttct tccgaagtat acggtcagac
    14281 taacattggc ggcaaaggcg gcacgcgtat tccaatctcc gggatcgccg gtgaccagca
    14341 ggccgcgctg tttggtcagt tgtgcgtgaa agaagggatg gcgaagaaca cctatggcac
    14401 tggctgcttt atgctgatga acactggcga gaaagcggtg aaatcagaaa acggcctgct
    14461 gaccaccatc gcctgcggcc cgactggcga agtgaactat gcgttggaag gtgcggtgtt
    14521 tatggcaggc gcatccattc agtggctgcg cgatgagatg aagttgatta acgacgccta
    14581 cgattccgaa tatttcgcca ccaaagtgca aaacaccaat ggtgtgtatg tggttccggc
    14641 atttaccggg ctgggtgcgc cgtactggga cccgtatgcg cgcggggcga ttttcggtct
    14701 gactcgtggg gtgaacgcta accacattat acgcgcgacg ctggagtcta ttgcttatca
    14761 gacgcgtgac gtgctggaag cgatgcaggc cgactctggt atccgtctgc acgccctgcg
    14821 cgtggatggt ggcgcagtag caaacaattt cctgatgcag ttccagtccg atattctcgg
    14881 cacccgcgtt gagcgcccgg aagtgcgcga agtcaccgca ttgggtgcgg cctatctcgc
    14941 aggcctggcg gttggcttct ggcagaacct cgacgagctg caagagaaag cggtgattga
    15001 gcgcgagttc cgtccaggca tcgaaaccac tgagcgtaat taccgttacg caggctggaa
    15061 aaaagcggtc aaacgcgcga tggcgtggga agaacacgac gagtaatgtc aggcacctga
    15121 atcgattgcc tgatgctgat cgcctgatgc gacgcttacg cgtcttatca ggcctacgag
    15181 ccaatgccat tttgtgtagg cgggataagg cgttcacgcc gcatccggca tcacgcagac
    15241 tctgaaaccg tgcgggggca accccgcaca catcaataat cccacccttc ccctgtgcta
    15301 cacttcgcgc cattccttac tgcttagagt ttgctatgag acgagaactt gccatcgaat
    15361 tttcccgcgt caccgaatct gcggcgctgg ctggctacaa atggttagga cgcggcgata
    15421 aaaacaccgc ggacggcgcg gcggtaaacg ccatgcgtat tatgctcaac caggtcaaca
    15481 ttgacggcac catcgtcatt ggtgaaggtg aaatcgacga agcaccgatg ctctacattg
    15541 gtgaaaaagt cggtactggt cgcggcgacg cggtagatat tgctgttgat ccgattgaag
    15601 gcacgcgcat gacggcgatg ggccaggcta acgcgctggc ggtgctggca gtaggcgata
    15661 aaggctgctt cctcaatgcg ccggatatgt atatggagaa gctgattgtc gggccgggag
    15721 ccaaaggcac cattgatctg aacctgccgc tggcggataa cctgcgcaat gtagcggcgg
    15781 cgctcggcaa accgttgagc gaactgacgg taacgattct ggctaaacca cgccacgatg
    15841 ccgttatcgc tgaaatgcag caactcggcg tacgcgtatt tgctattccg gacggcgacg
    15901 ttgcggcctc aattctcacc tgtatgccag acagcgaagt tgacgtgctg tacggtattg
    15961 gtggcgcgcc ggaaggcgtg gtttctgcgg cggtgatccg cgcattagat ggcgacatga
    16021 acggtcgtct gctggcgcgt catgacgtca aaggcgacaa cgaagagaat cgtcgcattg
    16081 gcgagcagga gctggcacgc tgcaaagcga tgggcatcga agccggtaaa gtattgcgtc
    16141 tgggcgatat ggcgcgcagc gataacgtca tcttctctgc caccggtatt accaaaggcg
    16201 atctgctgga aggtattagc cgcaaaggca atatcgcgac taccgaaacg ctgctgatcc
    16261 gcggcaagtc acgcaccatt cgccgcattc agtccatcca ctatctggat cgcaaagacc
    16321 cggaaatgca ggtgcacatt ctctgagtga tttgatcgat tgagccttcc agtccttcgg
    16381 gactggaatt tttttgttcg gagaacgaag ataaggcaag tcaatcaaaa caggagaaaa
    16441 acatggctga ttgggtaaca ggcaaagtca ctaaagtgca gaactggacc gacgccctgt
    16501 ttagtctcac cgttcacgcc cccgtgcttc cgtttaccgc cgggcaattt accaagcttg
    16561 gccttgaaat cgacggcgaa cgcgtccagc gcgcctactc ctatgttaac tcgcccgata
    16621 atcccgatct ggagttttac ctggtcaccg tccccgatgg caaattaagc ccacgattgg
    16681 cggcactgaa accgggcgat gaagtgcagg tggttagcga agcagctggc ttctttgttc
    16741 tggatgaagt accggactgc gaaacgctat ggatgctggc aaccggtaca gcgattggcc
    16801 cttatttatc gattctgcaa ctaggcaaag atttagatcg cttcaaaaat ctggtcctgg
    16861 ttcacgccgc acgttatgcc gccgacttaa gctatttgcc actgatgcaa gaactggaaa
    16921 aacgctacga aggcaaactg cgcattcaga cggtggtcag tcgggaaacg gcagcggggt
    16981 cgctcaccgg acggataccg gcattaattg aaagtgggga actggaaagc gcgattggcc
    17041 tgccgatgaa taaagaaacc agccatgtga tgctgtgcgg caatccacag atggtgcgcg
    17101 atacacaaca gttgctgaaa gagacccggc agatgacgaa acatttacgt cgccgaccgg
    17161 gccatatgac agcggagcat tactggtaag cggttactta tcgataaacg gcacgatgag
    17221 caaatccgca ctcatcttat tgatcatccc gcgatatgcc ggcatcaaac ggttgataaa
    17281 tgagtgatga tgaccacaga caaggaggtc gcactgctct ttttgcataa tttccagcag
    17341 tgtttccggc atttctccgc gttcaatacg cagttttgtc ttcggccatt gaatattttt
    17401 cgtcagttta tacagcttgt tatccgactt attcttcaac aattgaagaa tatcttctgt
    17461 tgcagggaag tagatacccg gatacaactc gcttaagcca tcatcaatat gaattaacgt
    17521 caggtgagcg tcattatgtc tggcgagctc cagggcttta ttcaccagta aggcatcttc
    17581 ttcattcccg gaaattgcca cgccaatgtg tttataagcc atgtttaact ccttctaaag
    17641 caactccatc aagctaacga acgcagtgat aactcaaaaa taatcatctc ggcctggcat
    17701 gagaaagtga aggccgcatc aagctcaact tgcccctcca gatccttaat cttataggcc
    17761 acgctggccc cttcatctgc agaggatgca gcggcggcaa gctctttcag tttggtcata
    17821 aaagactctg cttcttcgcg ggtggcaaaa aagcgcgaaa acttactggt acagttgtcg
    17881 ttatcaatca ccgtaccgat atcaatcgca catcctttag tactgcattt atctacgaca
    17941 tctttcatag ggcacctctg tatattcgcc tctctgtcga ttacgactta agaataatgc
    18001 gccgttaagg gaagaacaaa caagtaatga tcttatggat taccattttt tttgatattg
    18061 cttaaggtga agagttcaga aggattttcc gggtattgtc atttgcatcg tggaaataac
    18121 aatcccggaa aaaaatagca gtaagagata tattaaaggt taattacttg atttattgtc
    18181 ggctttatac ttcacatcct gagtatcttt accatattta ttttcgcctt gtgtgccaac
    18241 aaacgcacca agatcgataa gcatcatcac cagaatcaac gtcgggacaa aacgccccac
    18301 cgcccattgc cagacacccg gtaaaatcgc tcagttaccc gccagcagca tccacgccac
    18361 aatcatcaga aatgcccatg cgccggaacg cccgcgatca tgcaagcgct taacagttac
    18421 tgccgctgtt ggccagagca agcacacaag gcaaaacgcc gcggtctgaa tatcgagtag
    18481 attcttaccc gccagtgaga aaagcaccag catgcctgcg aaccacaggc ctatccaaat
    18541 ccagaaatca cggcgtccaa tacgcccttt aaatgagaat aaccattgct gtatggtcat
    18601 gtaagttcct tgatggttgt cttttccagg attctaccct tttgacaagg gcgacagata
    18661 tcgttttaat cggagccagt cataacaaaa ggtactgtca atgaagccag ggtgtacgct
    18721 gttttttctc ttatgttctg cattaaccgt tacaacaacg gcgcatgcac aaacaccaga
    18781 tacggcaacg accgcgcctt atctgctggc tggagcccct actttcgatc tctccatcag
    18841 ccagtttcga gaagacttta acagccagaa tcccagcctg ccactgaacg aatttcgtgc
    18901 catcgacagc agtcccgaca aagccaatct cactcgtgct gcaagtaaaa ttaacgagaa
    18961 cttgtatgct tctacagcgc tggagcgcgg taccttaaaa atcaaaagca ttcaaatgac
    19021 ctggctaccc atccaggggc cagagcaaaa agcagcgaaa gcgaaagctc aggaatacat
    19081 ggcagcagtg atccgcacac tcaccccatt aatgaccaaa acacaaagcc agaaaaaact
    19141 gcagtcgcta ctaacggcgg ggaaaaacaa acgttattac accgagacag aaggtgcact
    19201 gcgttatgtt gtcgcggaca acggcgaaaa ggggctgacc ttcgctgttg aaccgattaa
    19261 gctggcgcta tctgaatcgc ttgaaggttt gaataaatga caaaaagcaa agcctttgtg
    19321 ccgatgaatc tctatactgt ttcacagacc tgctgccctg cggggcggcc atcttccttt
    19381 attcgcttat aagcgtggag aattaaaatg cgacatcctt tagtgatggg taactggaaa
    19441 ctgaacggca gccgccacat ggttcacgag ctggtttcta acctgcgtaa agagctggca
    19501 ggtgttgctg gctgtgcggt tgcaatcgca ccaccggaaa tgtacatcga tatggcgaag
    19561 cgcgaagctg aaggcagcca catcatgctg ggtgcgcaaa acgtggacct gaacctgtcc
    19621 ggcgcattca ccggtgaaac ttctgccgct atgctgaaag acatcggcgc tcagtacatc
    19681 atcatcggcc actctgaacg tcgtacttac cacaaagagt ctgacgaact gattgcgaaa
    19741 aaattcgcgg tgctgaaaga gcagggtctg actccggttc tgtgcatcgg tgaaaccgaa
    19801 gctgaaaacg aagcgggaaa aactgaagaa gtttgcgcac gtcagatcga cgcggtactg
    19861 aaaactcagg gtgctgcggc attcgaaggt gcggttatcg cttacgaacc tgtatgggca
    19921 atcggtactg gcaaatctgc aactccggct caggcacagg ctgttcacaa attcatccgt
    19981 gaccacatcg ctaaagttga cgctaacatc gctgaacaag tgatcattca gtacggcggc
    20041 tctgtaaacg cgtctaacgc tgcagaactg tttgctcagc cagacatcga cggcgcgctg
    20101 gttggcggtg cttctctgaa agctgacgct ttcgcagtga tcgttaaagc tgcagaagcg
    20161 gctaaacagg cttaagtctg acaggtgccg gatttcatat ccggcactta ctttccttaa
    20221 ctcctcgcct taacgcaaaa tctcacactc atgatcctgg atttcctcgg ctgaagcacg
    20281 gttcagcgtc agtagatttc gttgtgtcgc cagcaacaca aacgaattat cactctgccg
    20341 taccatcgcc agcccgtagc ttcccatatg ttcccgcgcc tcaggcactt cttctgcaag
    20401 catcataaat gggctgcgtt gtaccagttc gctttccgtt acccgacgcg ccaggtattc
    20461 atgcccgcgc aaaccacctg gcagtggcaa ccagcggctg ctgatgttcg ccagattttt
    20521 atccagttgt tcgcgcacat caggacgaat acaagagata tgaatatgaa aatggttttg
    20581 cgtatgcccg gtgcgggagt tgatcgccaa agaaaccgcg cgatcgggaa ccggctggcc
    20641 gtattttttg ctcataaaat cacgcgcctg ccaggccaac caaaagaagt tcggcgttga
    20701 aggatcggtc aacaaaggac tttcagtacc gttaatacga tacgttggca tcaacagata
    20761 ttgcagtgga ccattaagat cttttaaaac cacgtatccg gcattgggtt tgacttccgc
    20821 acatggcgaa ggattttgat tttgctgctg attgggcaaa cattcctcaa ggacaatctt
    20881 acgtaatgta tccgactctt caccggttaa tttccagtaa ccaataccgg cagccacaac
    20941 ggcgataact atcatcacca aaaaaagaag acccgctttt ttcatctttt tttccctgta
    21001 cctcaaagag atgcaagggt aacgcaaaat cgtgacaaat aaaaaacccg gtcgcgagaa
    21061 accaccgggt ttgataatta tcctagggaa atcagcgttt gctgatctga tcgaacgtac
    21121 cgccgttagc aaaatgctct ttttgcgctt tcgtccagcc gccgaactct tcatcaatgg
    21181 tgaataactt cagctttgga aacgcatttt cgtacttttt cgctacttca gcgtcgcgcg
    21241 gacggtagta gtttttcgcg gcaatttcct gaccttctgg cgagtagaga tatttcaggt
    21301 aggcttccgc cacctctttg gtgccttttt tctcgaccac tttatcgacc accgacaccg
    21361 ttggctctgc gaggatagac tcactcggcg tgacgatttc gaatttatct ttccccagtt
    21421 cattcgctgc cagcagagct tcgttttccc aggcgatcag tacatcgcca atcccgcgct
    21481 cgacaaaggt gttagtggag ccgcgcgcgc cagaatccag aacttcgacg tttttataca
    21541 gtgcccgaac aaaatcctgt gcttttgcct gatcgttgtt gttgtgatgc agcgcgtagc
    21601 cccaggcagc caggtagttc cagcgtgcgc caccagagct tttcggatta ggcgtgatca
    21661 ccgaaacacc cggtttaatc agatcgttcc agtcatggat ctgcttcgga ttgcccttac
    21721 gcaccaggaa aacaatggtg gaagtgtacg gtgcggagtt atccggcaga cgtttgatcc
    21781 actctttatc aatccgcccg cgttccgcaa tcgcgtccac gtcataggcc agagccagcg
    21841 tgacaacatc agcttcaata ccgttgatta ccgacgtcgc ttgtttacct gaaccaccgt
    21901 gtgactgacg aatcaccacg ttatcaccag tttgctgttt ccagtgggcg ctgaatgcct
    21961 tgttgtactg ttcgtacaat tcgcgcgttg gatcatatga aacgttaaga agctgaatat
    22021 cctttgccat aacgctggtt gccgccagca aaaatgttaa ccctacgccc cacttgttca
    22081 tcgcccgact ctcttatgtt gtgttgtgat gagcaaagcg tgccagaagg ttaaccaaac
    22141 attaaagaat aaaaaaagat tggctataac ttgcgggtat atgttgaggg attaaaaagg
    22201 cggaagaaat ccgcctcata ttgctgacaa agtgcgcttt gtccatgccg gatgcggcgc
    22261 gaacgcctta tccggcctac aaaagtttgc aaattcaata aattgcagaa ttcatgtagg
    22321 cctgataagc gaagcgcatc aggcattttt gcttctgtca tcggtttcag gctaaaggaa
    22381 tctgcctttt tccgaaatca ttaatacagt tttttcgcgc agtccagcca gtcacctttg
    22441 aacggacgct tcatgttttc gatagcgtcg atgatgtcgt ggtgaaccag ctgttcgttc
    22501 tggataccta cacaacgacc gccgtaacct gccagcagca gatcgatagc gtaagcgccc
    22561 atacgggaag ccagaatacg gtcgtaaggc accggagaac caccgcgctg gatgtggccc
    22621 agcacagttg cgcgggtttc acgaccggtt tctttctcga tgaaatgcgc cagttcgtca
    22681 acatcacaca tatgttcggt aatcgccacg atcgcgtgtt ttttaccttt cgcgataccc
    22741 gctttgattt cgtttaccag gtcttcacgg ctgaattcaa cttccggaac tacaacgaat
    22801 tcacagccac cggcaatggc cgcagccagg gtcagatcgc cacaataacg gcccatcact
    22861 tccaccacgg aaatacgctg gtgagaagaa gaggtgtcac gcagacggtc gatcgcttct
    22921 acaacggtgc tcagcgcagt gaagaaaccg atagtgtagt cagtgccttt gatgtcgttg
    22981 tcgatagtgc ccggcagacc gatgcacggg aagcccattt cggtcagacg cattgcaccc
    23041 atgtaggaac cgtcaccgcc gataaccacc agcgcgtcga taccacgttt tttcaggttt
    23101 tcgatagcca cggcgcggat gttctcgtcg cggaattccg ggaaacgcgc agaaccgagg
    23161 aacgtaccgc cacggttgat catgtcagac acgctgtaac ggtctagctg taccatacgg
    23221 tcttcataca gacccagata gccgtcataa atacccatta cttccagacc ttctgtcagc
    23281 gcagaacgaa caaccccgcg aattgcggcg ttcatgcctg gcgcatcacc gccgcttgtc
    23341 aacacaccta ttttcttaat catgactacc tctgaacttt ggaatgcaaa atgaaatctg
    23401 ttgccggaag tcttcttgca catcgaagtg atccaacgaa tgtgcaaata gtataacaat
    23461 cacttcctgc tgaattgatt caggtcaggc caaatggcgg tattttatac acaaaatgcg
    23521 ggtctggctc tcttttatac tgattatgaa agcatagacc gtttaccctc cctgggtacg
    23581 acggaacagg ggtcctgatg gataatcaca tccgatcccg gaaaacgccg taaaatagcc
    23641 tgctctacct gatccgccac catatgtgcc tgaaccaaag gcagagagtc ttccatttcc
    23701 aaatgaatct gaataaagcg ggtcggccct gactgccgcg tgcgaagatc gtgagcgccg
    23761 ctaacacccg gccaggaagt cacgatatca ataatttctt gccgttcttc atcaggcaat
    23821 gcgcgatcca gtaatgactg taccgcctca tatcccatgc gtaacgcgct atataaaata
    23881 tagatgccga ttcccaatgc aaacagagca tcggcgcgat gccagccgta ccaagacaac
    23941 cccagcgcca gcagaattgc gccgttcatc ataacatcag actggtaatg tagcatatca
    24001 gcccgcaccg cctggctttg cgtccggcgc accacccacc gctgaaacga gacaaggata
    24061 atcgtacaaa ttagcgccac aattgtcacg ataaccccga cgcctggatc tgtcatcggt
    24121 gttggagata ccagatgttg aatacccgtc aaaaacagga atagtgccga accggagata
    24181 aacatacttt gcgccagcgc cgcgagggac tcagctttac cgtgaccaaa cgagtgatta
    24241 tcgtcggcag gttgcaggga atatcgcacc accagtaaat tcgtcaacga cgcgccgata
    24301 tccaccagcg aatccaccag cgcggcgaga atactcaccg acccggtata ccaccatgca
    24361 aaaattttaa tcagcaatag caacgaagcc atcgccgtcg cagcaatcgc cgcccgactg
    24421 accagccgtc cataagattg attcataaat actcccgcta tcaactgacg ctagtataac
    24481 ggaagcaaat catctgcaat gcattaagca gcaggcaaat tgaggataaa aaaaacccca
    24541 catcatgtgg gggaagacag ggatggtgtc tatggcaagg aaaacagggt ttactactgg
    24601 gaacgtgagt tgctactact caatagcttc aacgatgaac ttttttgcca ttgcgtcacg
    24661 tcacgcaact gctccattcg ttgttgatgt ttctcgttta aaaccgcttg ctgctccggc
    24721 gttaacaggc gatacatttg gttgcggact tttgccatct caacctgacg agcaatttgc
    24781 tcattcgcca ttttttctgc ctgtgcgcgc acagcgtttt catcaaaatt ttctgcggtg
    24841 acaaggcgat gcattgtctc cagttcgcta acattaacag gaggctgttc gtgccgggcc
    24901 tgttgcataa gatctcgcat ctgctgacgc tgatgttcgg ttaaacttat gccgtcgaac
    24961 atatggctct gcgtactgcg ctgcgtaagt tcttcacccg gatgccagtt atcgcctgaa
    25021 ccgacttcag cagcgtggct taatgaactg actgccagcg ttgaggccat gacggcagcg
    25081 gtaactatgc gcatcatttg ctcccaaaat ctttctgtcg cgattcaacg atagagagtt
    25141 tacgattcag gctgcaaaca tgcgtcaggg ggtgtaaaac aacgtaaagt catggattag
    25201 cgacgtctga tgacgtaatt tctgcctcgg aggtatttaa acaatgaata aaatcctgtt
    25261 agttgatgat gaccgagagc tgacttccct attaaaggag ctgctcgaga tggaaggctt
    25321 caacgtgatt gttgcccacg atggggaaca ggcgcttgat cttctggacg acagcattga
    25381 tttacttttg cttgacgtaa tgatgccgaa gaaaaatggt atcgacacat taaaagcact
    25441 tcgccagaca caccagacgc ctgtcattat gttgacggcg cgcggcagtg aacttgatcg
    25501 cgttctcggc cttgagctgg gcgcagatga ctatctcccg aaaccgttta atgatcgtga
    25561 gctggtggca cgtattcgcg caatcctgcg ccgttcgcac tggagcgagc aacagcaaaa
    25621 caacgacaac ggttcaccga cactggaagt tgatgcctta gtgctgaatc caggccgtca
    25681 ggaagccagc ttcgacgggc aaacgctgga gttaaccggt actgagttta ccctgctcta
    25741 tttgctggca cagcatctgg gtcaggtggt ttcccgtgaa catttaagcc aggaagtgtt
    25801 gggcaaacgc ctgacgcctt tcgaccgcgc tattgatatg cacatttcca acctgcgtcg
    25861 taaactgccg gatcgtaaag atggtcaccc gtggtttaaa accttgcgtg gtcgcggcta
    25921 tctgatggtt tctgcttcat gataggcagc ttaaccgcgc gcatcttcgc catcttctgg
    25981 ctgacgctgg tgctggtgtt gatgttggtt ttgatgttac ccaagctcga ttcacgccag
    26041 atgaccgagc ttctggatag cgaacagcgt caggggctga tgattgagca gcatgtcgaa
    26101 gcggagctgg cgaacgatcc gcccaacgat ttaatgtggt ggcggcgtct gttccgggcg
    26161 attgataagt gggcaccgcc aggacagcgt ttgttattgg tgaccaccga aggccgcgtg
    26221 atcggcgctg aacgcagcga aatgcagatc attcgtaact ttattggtca ggccgataac
    26281 gccgatcatc cgcagaagaa aaagtatggc cgcgtggaac tggtcggtcc gttctccgtg
    26341 cgtgatggcg aagataatta ccaactttat ctgattcgtc cggccagtag ttctcaatcc
    26401 gatttcatta acttattgtt tgaccgcccg ctattactgc tgattgtcac catgttggtc
    26461 agtacgccgc tgctgttgtg gttggcctgg agtctggcaa aaccggcgcg taagctgaaa
    26521 aacgctgccg atgaagttgc ccagggaaac ttacgccagc acccggaact ggaagcgggg
    26581 ccacaggaat tccttgccgc aggtgccagt tttaaccaga tggtcaccgc gctggagcgc
    26641 atgatgacct ctcagcagcg tctgctttct gatatctctc acgagctgcg caccccgctg
    26701 acgcgtctgc aactgggtac ggcgttactg cgccgtcgta gcggtgaaag caaggaactc
    26761 gagcgtattg aaaccgaagc gcaacgtctg gacagcatga tcaacgatct gttggtgatg
    26821 tcacgtaatc agcaaaaaaa cgcgctggtt agcgaaacca tcaaagccaa ccagttgtgg
    26881 agtgaagtgc tggataacgc ggcgttcgaa gccgagcaaa tgggcaagtc gttgacagtt
    26941 aacttcccgc ctgggccgtg gccgctgtac ggcaatccga acgccctgga aagtgcgctg
    27001 gaaaacattg ttcgtaatgc tctgcgttat tcccatacga agattgaagt gggctttgcg
    27061 gtagataaag acggtatcac cattacggtg gacgacgatg gtcctggcgt tagcccggaa
    27121 gatcgcgaac agattttccg tccgttctat cggaccgatg aagcacgcga tcgtgaatct
    27181 ggcggtacag gattggggct ggcgattgtt gaaaccgcca ttcagcagca tcgtggctgg
    27241 gtgaaggcag aagacagccc gctgggcggt ttacggctgg tgatttggtt gccgctgtat
    27301 aagcggagtt aaactccgca tttgtaggca ggataaggcg tttacgccgc atccggcatt
    27361 tgagcaggat gcctgatgcg acgctgatag cgtcttatca ggcctacact ccgggggtta
    27421 ttttccccac aaccggcggg aaaaatcttc gatcttgccg ctcagtcggc gcttttgcat
    27481 cgtccgcgtc cagcttttcg ataacccggc ggcggagagt aaacggtgat actgatcgtc
    27541 atcaaacggc atatgccatg cgatggcggc agcctcctgc acggtcacat cgctgacacg
    27601 ggaaaccaac tccagcggcg cgtccgcaga cactttccct ggcgctatca cgctatacaa
    27661 ccaccccacc ttacctgtgt tttgcatcaa ctgcgctata tcgctgatat caaaatggta
    27721 attgagttta tagcaaggcg agcgcggctg gctgacctga attaatgcct ctccccagcg
    27781 gaaaatatcg cccatatgaa cattactttc cgtgagaccg tcggttgaga gattttcgcc
    27841 aaacgcaggc gcaacaaaca actccgcctg ttccggaaat tcccgcaccc agtagagata
    27901 gtgctcacga ggataatgac acagcgctct gtctggcccg ccgtgaacct ttttctccgc
    27961 ctgctcgtca ccttccagcc ccagctccgt cagcatcaac tcaccatcaa cctggatttt
    28021 ggcaattgcg ctgggtttgc cttcgggata agcctgaatc ttgcctgtgt atacatcaac
    28081 cggatatcgc atctgttccc tctccacgag aaatttcccc attaga
//
LOCUS       DAZSNM010000026        28089 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZSNM010000026 DAZSNM010000000
VERSION     DAZSNM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28089
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.026"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            233..1003
                     /locus_tag="ACHW42_001012"
     CDS             233..1003
                     /locus_tag="ACHW42_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR8942936.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            994..1467
                     /locus_tag="ACHW42_001013"
     CDS             994..1467
                     /locus_tag="ACHW42_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR8942937.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWPTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1574..2113
                     /gene="dnaT"
                     /locus_tag="ACHW42_001014"
     CDS             1574..2113
                     /gene="dnaT"
                     /locus_tag="ACHW42_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR8942938.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2116..2853
                     /gene="dnaC"
                     /locus_tag="ACHW42_001015"
     CDS             2116..2853
                     /gene="dnaC"
                     /locus_tag="ACHW42_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR8942939.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2902..3396
                     /gene="yjjA"
                     /locus_tag="ACHW42_001016"
     CDS             2902..3396
                     /gene="yjjA"
                     /locus_tag="ACHW42_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR8942940.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3636..5927
                     /gene="opgB"
                     /locus_tag="ACHW42_001017"
     CDS             3636..5927
                     /gene="opgB"
                     /locus_tag="ACHW42_001017"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR8942941.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6065..7087)
                     /gene="lgoD"
                     /locus_tag="ACHW42_001018"
                     /pseudo
     CDS             complement(6065..7087)
                     /gene="lgoD"
                     /locus_tag="ACHW42_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7226..8140
                     /locus_tag="ACHW42_001019"
     CDS             7226..8140
                     /locus_tag="ACHW42_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR8942942.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8355..9716
                     /locus_tag="ACHW42_001020"
     CDS             8355..9716
                     /locus_tag="ACHW42_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8942943.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9765..11429)
                     /gene="tsr"
                     /locus_tag="ACHW42_001021"
     CDS             complement(9765..11429)
                     /gene="tsr"
                     /locus_tag="ACHW42_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFR8942944.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEISSASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11548..11994)
                     /gene="hpaR"
                     /locus_tag="ACHW42_001022"
     CDS             complement(11548..11994)
                     /gene="hpaR"
                     /locus_tag="ACHW42_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR8942945.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12266..13555
                     /gene="hpaG"
                     /locus_tag="ACHW42_001023"
     CDS             12266..13555
                     /gene="hpaG"
                     /locus_tag="ACHW42_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR8942946.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13552..15018
                     /gene="hpaE"
                     /locus_tag="ACHW42_001024"
     CDS             13552..15018
                     /gene="hpaE"
                     /locus_tag="ACHW42_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR8942947.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15020..15871
                     /gene="hpaD"
                     /locus_tag="ACHW42_001025"
     CDS             15020..15871
                     /gene="hpaD"
                     /locus_tag="ACHW42_001025"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR8942948.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15881..16261
                     /locus_tag="ACHW42_001026"
     CDS             15881..16261
                     /locus_tag="ACHW42_001026"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR8942949.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16329..17132
                     /gene="hpaH"
                     /locus_tag="ACHW42_001027"
     CDS             16329..17132
                     /gene="hpaH"
                     /locus_tag="ACHW42_001027"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR8942950.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17143..17946
                     /gene="hpaI"
                     /locus_tag="ACHW42_001028"
     CDS             17143..17946
                     /gene="hpaI"
                     /locus_tag="ACHW42_001028"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR8942951.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            17968..19323
                     /gene="hpaX"
                     /locus_tag="ACHW42_001029"
     CDS             17968..19323
                     /gene="hpaX"
                     /locus_tag="ACHW42_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR8942952.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19333..20223
                     /gene="hpaA"
                     /locus_tag="ACHW42_001030"
     CDS             19333..20223
                     /gene="hpaA"
                     /locus_tag="ACHW42_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR8942953.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20250..21187
                     /locus_tag="ACHW42_001031"
                     /pseudo
     CDS             20250..21187
                     /locus_tag="ACHW42_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21414..22972
                     /gene="hpaB"
                     /locus_tag="ACHW42_001032"
                     /pseudo
     CDS             21414..22972
                     /gene="hpaB"
                     /locus_tag="ACHW42_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            22990..23502
                     /locus_tag="ACHW42_001033"
     CDS             22990..23502
                     /locus_tag="ACHW42_001033"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR8942954.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            23895..26045
                     /gene="btsT"
                     /locus_tag="ACHW42_001034"
     CDS             23895..26045
                     /gene="btsT"
                     /locus_tag="ACHW42_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR8942955.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26095..26298
                     /locus_tag="ACHW42_001035"
     CDS             26095..26298
                     /locus_tag="ACHW42_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR8942956.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26309..27265
                     /gene="yjiA"
                     /locus_tag="ACHW42_001036"
     CDS             26309..27265
                     /gene="yjiA"
                     /locus_tag="ACHW42_001036"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR8942957.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27334..>28089)
                     /locus_tag="ACHW42_001037"
     CDS             complement(27334..>28089)
                     /locus_tag="ACHW42_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFR8942958.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6831 a         7364 c         7085 g         6809 t
ORIGIN      
        1 ttatcctgac ccgtaaaggc taaacaataa attcattata gtgataatat tcatgtgata
       61 gtttgtttgc ggcgagagat aattcgcttt ttatcaccga gcttgtccca ataaccagga
      121 ttaatcagcg caattttgat cttaacctac cgcaaaacaa ccgtgcttca tgatacaaat
      181 ggattctcat tatctgaaca atacgcagca cgtctatgac aaagggcgag ttatgcaaac
      241 tgagcaacag cgagccgtaa cgcggctttg tatccagtgt ggattatttc ttttgcaaca
      301 tggtgcggaa agcgcgttgg ttgatgagct ttcctcacga ctgggtcggg cgctgggaat
      361 ggacagcgtc gaaagttcta tctcttcgaa cgccatagtg ctgacaacta ttaaagatgg
      421 gcaatgcctg acatcgacac gtaaaaatca cgatcgcggc attaatatgc atgtggtgac
      481 tgaagtccag cacattgtga ttcttgcgga gcatcatctg ctggattaca aaggcgtaga
      541 gaaacgattt agccaaattc aaccattacg ttacccaaga tggctggtag ccttaatggt
      601 tggcctttct tgcgcctgtt tctgtaaact caataacggt ggctgggatg gtgccgtcat
      661 caccttcttt gccagtacga ccgcgatgta tatccgccag ctgctggcac aacgtcatct
      721 tcatccacag atcaactttt gccttaccgc tttcgccgcc accaccattt ccggattgct
      781 tttgcaactc cccactttca gcaatacccc caccattgcg atggccgcca gcgttctgct
      841 gctagtgccg ggctttccgt tgattaatgc cgtcgccgat atgtttaaag gccacatcaa
      901 taccggactg gcacgctggg cgatcgccag tctgctgaca ctggctacct gcgtcggcgt
      961 agtgatggca ctgacgattt gggggctacg cggatgggtg tgatcgaatt tctgttagcg
     1021 ttggcgcagg atatgatcct cgccgccatt cctgcggtcg gctttgcgat ggtgttcaac
     1081 gttcccgtgc gggcgttacg ctggtgtgcg ctgcttggct cgataggtca tggttcccga
     1141 atgatcttga tgaccagcgg gttgaatatt gagtggccaa cctttatggc ttctatgctg
     1201 gtcggtacca ttggtattca atggtcgcgc tggtatctgg cgcatccgaa agtgtttacc
     1261 gtggcggccg ttatccctat gttcccgggc atatcggctt ataccgcaat gatttcgtcg
     1321 gtaaaaatca gccagttagg ttacagcgaa ccgttgatga ttaccctgtt aaccaacttt
     1381 cttacagctt catcgattgt tggtgcgtta tccatcggtc tttccattcc tggattatgg
     1441 ttgtaccgca agcgccctcg cgtataaaat tgcctcgctt aagcacacgg atgagagaca
     1501 gcctcctctc ctccgtgtgt tactataaaa gttatctccc ttctcgttca tcgttccata
     1561 tttgagaaac agtatgtctt ccagagtttt gaccccggac gtcgttggta ttgacgccct
     1621 ggtacacgat caccaaaccg ttctggcaaa agctgaaggc ggtgtggttg ccgtatttgc
     1681 taacaatgcc ccggcgtttt atgccgtcac gcctgcacgc ctggctgaac tgctggcgct
     1741 ggaagaaaag ctagcgcgtc cgggaagcga tgtcgctctg gacgatcaac tctatcagga
     1801 accgcaagcc gctcccgttg ctgtacccat ggggaaattc gccatgtatc cggactggca
     1861 acccgatgcc gattttatcc gcctggcggc gctatggggc gtggcgctaa gagagccggt
     1921 gaccaccgaa gaactggcct cattcattgc ctactggcag gcggaaggta aagtctttca
     1981 ccatgtgcag tggcaacaaa aactggcgcg cagcctgcaa atcggtcgtg ccagcaacgg
     2041 cggactgccg aaacgagatg tgaatacggt cagtgaacct gacagccaaa ttccaccagg
     2101 attcagaggg taacgatgaa aaacgttggc gacctgatgc aacgcctgca aaaaatgatg
     2161 cctgcccata tcaaacctgc attcaaaacg ggtgaagagc tactggcctg gcagaaagaa
     2221 caaggggcga tccgctccgc cgctctcgaa cgtgaaaatc gggcgatgaa aatgcagcgc
     2281 acctttaacc gctccggtat tcgtccactg catcagaact gctcctttga gaactatcgc
     2341 gttgagtgtg aagggcagat gaatgcgtta agcaaagcgc gccagtatgt cgaagagttt
     2401 gacggcaaca tcgccagctt catcttttct ggtaagccag gaaccggcaa aaaccatctg
     2461 gcggcggcaa tctgcaacga gctgctgcta cgcggtaaat ccgtattgat catcaccgtg
     2521 gccgatatta tgtcggcgat gaaagatacc ttcaggaata gcggtaccag cgaagaacaa
     2581 ctgcttaacg atctgagcaa cgttgatctg ctggtgatcg atgagatcgg cgtgcagacc
     2641 gaatcgaaat acgaaaaagt gatcatcaac cagatcgtcg atcgccgctc ttcttccaaa
     2701 cgcccaaccg ggatgctgac caacagtaat atggaagaga tgaccaagtt gctgggcgaa
     2761 cgcgtgatgg accgtatgcg cctgggtaac agtttatggg tgatcttcaa ctgggatagc
     2821 taccgtagcc gggtaacggg taaagagtat taatccccgt ttctggcggt atactgcgac
     2881 acaattccac cttgagtaat gatgatgaaa actattaaac atcttctgtg ctgtgccatt
     2941 gccgccagcg cattaatttc caccggggtg catgctgcgt cctggaaaga tgcgctctcc
     3001 agcgccgcca gcgaacttgg caaccaaaac agcacgacac aggaaggcgg ttggtcgctc
     3061 gcgtcattaa ctaacttgct tagcagcgga aaccaagcct taagcgcaga taacatgaac
     3121 aatgccgcag gcattctgca atactgcgcg aagcaaaagc tggcttcggt aaccgatgcc
     3181 gaaaacatca agaaccaggt gctggaaaag ctgggcctga acagtgaaga gcaaaaagaa
     3241 gacaccaact atctggacgg tattcagggt ttgctgaaaa caaaagatgg tcagcaactc
     3301 aatctggata acatcggaac gactccgctg gcagaaaagg tgaaaaccaa agcctgcgat
     3361 ctggtgttaa agcaggggct gaacttcatt tcctgaaccg cgaaatgtta cccggctgac
     3421 gcggctctct cctgcccttt tccctgctcc tggacggttt tccccctgtt acacgggaag
     3481 cgaccgcgat caaaaaagtc tgtttataaa ctaattccta acaacatcgc atttttttga
     3541 cactagaatt gcagcaatat ggcgacgcca ttacattgtg cttctcaaaa agcttaaacg
     3601 ttggctaaac ggcccggttt agtgaggatc gcccattgtc agaactactc tctttcgccc
     3661 tttttctcgc ctctgtgctg atttacgcat ggaaagcggg acgtaacacc tggtggtttg
     3721 cagccacgtt aacggtgctg gggctatttg tcgttttaaa tatcaccctg tttgccagcg
     3781 actattttac tggcgatggt attaacgacg cggttctcta taccttaacc aacagcctga
     3841 ccggtgctgg cgtcagcaaa tacatcctgc cgggtatcgg cattgtgctg gggctgacgg
     3901 cggtgttcgg tgcgctgggc tggatcctgc gccgtcgtcg tcatcatccg caccattttg
     3961 gttacagcct gctggcgctc ttactggcgc tgggttcggt ggacgccagc ccggcatttc
     4021 gtcagataac ggaactggtg aaatcccagt cacgcgatgg cgacccggac tttgcggctt
     4081 attataaaga gccgtcgaaa actatccctg acccgaaact caacctggtt tatatctacg
     4141 gcgaaagtct cgagcggacc tattttgata acgaagcttt cccggatctc acgcctgaac
     4201 tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac acagcagctg ccaggaacgg
     4261 attacacgat tgcgggcatg gtggcttctc agtgcggcat accgctgttc gccccctttg
     4321 aaggcaacgc ctccgcctct gtctccagct tcttcccgca gaacatctgt ctgggcgata
     4381 tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg cgcaaatctg cgttttgccg
     4441 gtaaagatgt gttcctgaaa tcgcacggct tcgaccactt atacggctca gaagagctga
     4501 aaagcgtggt agccgacccg cactatcgca acgactgggg attctacgac gataccgttc
     4561 tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc aggtcagcga ttctcactgt
     4621 ttaccctgac agtcgatacc catcacccgg atggttttat ctcgcgcacc tgtaaccgca
     4681 aaaaatatga ttttgacggt aagccgaatc agtcattcag cgcggtaagt tgcagccagg
     4741 agaatatcgc gacgtttatc aacaaaatca aagcgtcacc gtggtttaaa gatactgtca
     4801 tcgtcgtctc ttctgaccat ttagcgatga acaacacggc gtggaaatac ctcaataagc
     4861 aggatcgcaa taacctgttt tttgtcattc gtggcgacaa gccgcagcaa aagacgctgg
     4921 cagtgaagcg taacacgatg gataacggcg cgacggtgct ggacattctc ggtggcgata
     4981 actatctcgg acttggtcgt agcagtttat ccgggcagtc gatgtcggaa atcttcctca
     5041 atatcaaaga gaaaacattg gcgtggaagc cagatatcat ccgcctgtgg aaattcccta
     5101 aagagatgaa agagttcacc atcgaccagc agaaaaacat gattgccttc tcgggtagcc
     5161 atttccgttt gccgctgctg ttgcgggttt cagacaaacg cgtggaaccg ctgccggaaa
     5221 gcgaatactc agcaccgctg cgtttccagc tggccgattt cgctccacgc gacaatttcg
     5281 tctgggttga cagttgctac aagatggcac aactctgggc tccggaactg gcactttcca
     5341 ccgactggtg tgtctcgcag gggcagcttg gcggtcagca aattgttcag catgttgaca
     5401 aaacaacatg gaagagcaag acggcattta aagatacggt catcgacatg gcgcgttaca
     5461 aaggcaatgt cgatacgctg aagattgttg ataacgatat tcgctacaaa gccgacagtt
     5521 tcatctttaa cgtcgccggt gcgccagaag aggtgaaaca gtttagcggg atttcccgtc
     5581 cggagtcgtg gggccgctgg tccaacgcgc agctgggcga tgaagtaaaa atcgagtaca
     5641 agcatccgct gccgaagaaa tttgacctgg tgattaccgc caaagcatac ggcaataacg
     5701 ccagccgtcc tattccggta cgcgtaggca atgaagaaca aactcttgtg ctgggcaatg
     5761 aagtgaccac caccacactg catttcgata acccaaccga tgccgacaca ctggtaattg
     5821 tgccgccgga acctgtctca accaacgaag ggaatatcct cggacactcg ccgcgtaagc
     5881 tcgggatcgg catggtggaa attaaagtgg tagaacgtga agggtaattc ctgactgatg
     5941 tgtaggtcgc atagggtgcg acctacacgc cttctccggc ttacaacaca ttattaagaa
     6001 acaaaaaaaa cagcgcccaa cacttccccg gacgctgcat ggcaacttta ataatctcgc
     6061 cttatcagaa agtaattaca cccttaatca actcacgatt gttaatcaca tcgcgctcgt
     6121 aggtttctgc cagcgtggcg aacggatagc gatgggttaa catcatgtcg gcagtgattt
     6181 tcccttccgc catcagtcga ccgactttag cgaaatcttc cggcgtggcg ttgcggctgc
     6241 ccatcatcgt cgtttctttt ttatggaatt ccggatcgga gaactgcaac tcacctttaa
     6301 acagaccgac aaataccacc gtgccgccgt gacgaatcag attcacggta ttattcatcg
     6361 catgttgatt acctgtcgcg tcgatcactt tctgcgccag cgaaccacca aactgcgccc
     6421 gcagctgcgc gtcaaaatct tcggctgacg ggtccagcac gggcaactcc agacgcgttg
     6481 ccacatgttc acggcgcgcc ggactggtat ccgccaccac cacctgtgcg ccatcggctt
     6541 tagcgattgc cgccgcgccc agaccgattg gccccgcccc gaccaccagc acctgctcgc
     6601 cgggagcaat ggctgcgcga cgcaccgcat gagcgctaat agcgaaaggt tcaatcaatg
     6661 cggccgcctg cgggtcaata ccgtctgcgg gcaaaatgtt cgccaccggc acgctcaaat
     6721 actcactaaa accgccatcc tgatgcacgc caatgactga aattttttca cagcagttgg
     6781 tacgcccgct tttacacgcc gggcattgct gacaggcaac ataagggatc acagcaactt
     6841 gctgaccatt tttaagatca gcaatatttt tacccagccc aacaatctcc ccacatattt
     6901 catggcctaa aacccgtgga taactaaaaa atggttgatt tccaccctag gcatgaatat
     6961 cggtaccgca aatcccgaca gacttaattt ttattaatgc ttcattgtca cccggaatag
     7021 gtatctcgcg ttgtttccag actaattctt tcggctgctg gcaaattaaa acattcatcg
     7081 tagacatgat tcggctatcc attttttgtt gccttagata tcaatgaaaa tgaaaaatgg
     7141 cagcggaacg gaaaatctgt tttgtgaaaa cacacgcata aaaatgtttt aaatcgggtt
     7201 ttaatggact cacaaggagg aaaatatgag tcgttcacaa aatttacgcc acaatgtgat
     7261 taaccaggtc attgatgata tggcgcgggg tcatatccct tccccacttc catcgcaaag
     7321 tgcgctggcg gaaatgtaca acatcagccg cactacagtg cgtcatattc tcagccaatt
     7381 acgcgaatgc ggcgtcttaa cgcaggttgg aaacgactat gttattgcgc gtaaacctga
     7441 ccatgatgac ggtttcgcct gtactaccgc ttcaatgagt gaacaaaaca aagtgtttga
     7501 gcaggctttt ttcacgatga taaaccagcg tcagttacgc cctagggaaa ctttttctga
     7561 actgcaactg gctcgggccg cgggtgtcag cccggtggtt gtaagagaat atcttttaaa
     7621 attcggccgt tataatctta ttcaaagcga aaagcgcggc cagtggagca tgaagcaatt
     7681 cgatcaatcc tacgccgagc agctgtttga gctacgcgaa atgctcgaaa cacattcact
     7741 tcagcacttt ctcaacctgc ctgatcatga tccccgctgg ctgcaggcga aaaccatgct
     7801 agaacgtcat cggctactgc gtgacaacat cggcaatagc tttcgcatgt tctcgcaact
     7861 tgacagagat tttcactcct tactgctttc agctgccgac aatatctttt ttgatcaatc
     7921 gcttgagatc atctcagtca tctttcattt tcactatcaa tgggacgaaa gcgatcttaa
     7981 acagcgcaat atcattgccg tcgatgaaca tatgaccatt ctcagcgctc tgatttgccg
     8041 cagtgacctt gatgccaccc ttgcgctccg taaccatttg aattcagcta aacaatcaat
     8101 gattcgatcc attaatgaga acacgcgtta tgctcattaa gtacggatta aaaacagcaa
     8161 actattagcg ggtgcacggt tttatcaaaa gataaatacc gggaaattcc cgcttaccta
     8221 tgctcacaat cagtcgacta aaaatgtttt tgtgagcggt agtaaagtcc taaaacttta
     8281 acctgccgtt aatgtcaacc ttgcgtaatg cacaattacg taaacaatta aaattataaa
     8341 tacaggagtc cggcgtggaa aaagaaaata tcaccctcga tccgcgttct tcatttactc
     8401 catcttcgtc ggcagatatt cccgtgccac cagatggatt agttcaacgc agtacccgaa
     8461 ttaaacgcat tcaaaccacc gccatgttgt tattattttt tgcggcggta atcaattatc
     8521 tcgaccgcag ttcgctgtcg gtagcaaatt taacgattcg tgaagaattg ggattaagtg
     8581 ccaccgaaat cggcgctttg ctctccgtgt tttcactcgc ttacggcatt gcgcaacttc
     8641 cttgcggccc actattggat cgtaaaggcc cacgcctgat gctgggactg gggatgttct
     8701 tctggtcact gttccaggcc atgtctggca tggtgcacag ctttacgcag ttcgtgttgg
     8761 tgcgtatcgg tatggggatt ggtgaagcgc cgatgaaccc atgcggtgta aaagtcatta
     8821 acgactggtt caacatcaaa gagcgcggac gcccgatggg cttcttcaac gcagcttcta
     8881 ccattggcgt tgctgtaagc ccaccgattc tggcggcaat gatgctggtg atgggctggc
     8941 gcgggatgtt tattaccatt ggtgtactgg ggatttttct cgccatcggc tggtatatgc
     9001 tctatcgcaa ccgcgagcac gtagaactga ctgccgttga acaagcttat ctcaatgcag
     9061 gtagcgtcaa tgcccgccga gatccgctca attttgccga atggcgcagc ctgttccgta
     9121 accgcacaat gtggggaatg atgctcggat tcagtggcat caactacact gcgtggctgt
     9181 atctggcctg gcttcctggt tacctgcaaa cagcctataa cctggattta aaaagcacag
     9241 ggttgatggc ggctatccct ttcctgtttg gggctgccgg gatgctggtc aacggttacg
     9301 ttactgactg gctggtcaaa gggggaatgg ctccgattaa aagccgtaag atctgcatta
     9361 ttaccgggat gttctgttct gccgccttta cgctgatagt accacaagcg acaacatcca
     9421 tgatggcggt tctgctgatt ggcatggcac tgttctgtat tcactttgcc ggaacatcct
     9481 gctggggctt gatccacgtc gcagttgctt ctcgcatgac tgcgtcggtg ggcagtatcc
     9541 agaactttgc cagcttcatc tgcgcctctt tcgcgccgat cattactggt tttattgttg
     9601 atacaaccca ctcattccgt ctggcactaa tcatttgcgg ttgcgtcacc gcagcgggag
     9661 cactggcgta catcttcctg gttcgccagc cgatcaacga cccgcgtaaa gattaaactc
     9721 cctatgcccg ataacatttt gcttatcggg catttttatg gcgattaaaa cgtttcccag
     9781 ttctccccgc tatctgccac tgccatttta cgcggcgtag ctggcgtcac ggtttttacc
     9841 acggccgatg tttcacgctg ctgttgctgc tgttgctgaa tccggaacac tgccacagct
     9901 tcggtcaggc gactggcctg ctcttccagc gcggcggcgg cagcggcaga ttcttccacc
     9961 agcgcggcgt tctgttgagt tacccggtcc atctcagcaa ccgctaagcc aacctgatcg
    10021 ataccacggc tctgctcatc agaagcagaa gatatttcgc ccataatgtc cgtcacgcgg
    10081 gtcacagcac tgacaatctc cgccattgtt tccccggcgc tttcgaccag cgtagagcca
    10141 acatccactt tccccaccga gtcttcaatc aggcttttaa tttcacgaac cgcctgggcg
    10201 ctacgctggg ccagattacg cacttctccc gcaaccaccg caaaaccacg tccttgctca
    10261 cccgcacgcg ctgcttcaac cgccgcgttc aaagccagaa tattggtctg gaaggtaatg
    10321 ccgtcaatta cgctgataat atcggcgatt ttctgcgaac tggtggagat atcgcgcata
    10381 gtctgcacca cgttatctac cactttgccg ccgcgttgcg ccgtttcaga agcacttaat
    10441 gccagatggc tggcctggcg cgcattctcg gcgttctgtt tcaccgttgc ggtcagttgc
    10501 tccatgctgg ctgccgtctc ttccagcgaa gcggcctgtt gctcggtgcg cgaagagaga
    10561 tcattattgc cggtagcgat ttcgctggca ccgctataga tggcattggc cccgttgcgc
    10621 acatcaccga cggtacgcat cagctctccc tgcatatggc gcaaactctc tgccagttgc
    10681 cccatctcat tagagccatc cacctcaatc ggtttcacca gatcgccgcc tgcaatatga
    10741 cgaatgctgt caatcaggcg attcattggc gctaccagcg aggctttaat accgaaccag
    10801 acggcgaaga tgaccgccag tacgacgatc atcacgccca ccagaatcca catcgcctgg
    10861 ctgtaggagg cattgttatc gctgacggcg atatcataga gccgatcgtt ttgctccatg
    10921 taagccacat actgcttctc gaaaccgtcc tgatatccct gggtcggctg atcaaagaac
    10981 tcgttgattt tgcctgcacc taacagttgg atcagctccg ccagcgcatt gtgataaata
    11041 tcgtaattac gtttgatctc tgccgctgcg gcggtgctct gacgcgggtc acgcggcaac
    11101 gcttcgtaat ccgcccagtt tttttccgcc tgtttcagcg aaatactggc actctgcatc
    11161 agctcagcaa cggttgaacc gctaccaata ttattctgat ccatcatgta gcggataccc
    11221 gcgcggttga gggtgttacg cgtctgcaac aacgcgaccc agctgccatt cagcgtgggt
    11281 tgctgctggc gaatggtttg taaaacagtg aaattttctt tgtcattctt taaggcatta
    11341 aaaaacagac cgcctgatgt cagttgtaaa aggccaaaaa cggccaaaac cagcagtaag
    11401 ctggtcacga tcttgatacg ttttaacatg gtttctcttt cctgtggacc gatgcaagat
    11461 tttcggcctg gaaagcaaaa actttatgaa aaggtgtaac ccgccatcca tggaaaaacc
    11521 aggcgctggt tatcagatac taaaaagtta ttcattatcg cccgggatgt cttcttgtcg
    11581 ggaattgccc agagcaataa attcctctaa cagatgggtt aactgttgca ttttctcggc
    11641 agtaaactga gcttcaatct gccgataggc ttcttcaatc tgtgtctgag cgcggttata
    11701 cagcgcctgc ccctctttgg tcagcgagat atagagcttc cgctgatcat tgatcggctt
    11761 taatcgcaac actaaaccgt cgcgttccat ccgcgtgagg atcccggtca ggcttgggcg
    11821 caaaatgcag gcgcgatacg ccagatcgtg aaaatccatt gatgggcttt ccgccagaat
    11881 acgcacgatg cgccactgtt gctcagtcaa attatgccgt ttaacgattg ggcgaaaata
    11941 actcatcgcc gcttctcgcg cctggagcaa cgcaatggtt agtgagtcgt gcattatctt
    12001 tcccctgaaa aattcaaaat cattaatata gaaacaatta atcatcacgt taccgggact
    12061 gagctgcccc cgcaattctt cattttatca aaattgatga tattttatta acttactgat
    12121 atttaatatt ttattatctt taaatgtaaa tagtttgtta attagatcac atttatatca
    12181 cttacttttg ccaaactacg caatcgggaa taaaagtaat cattaacata ttaatgatta
    12241 agatgatcga atccgaggag tggtaatgaa aggcactatc ttcgccgtag cgttgaacca
    12301 tcgcagccaa cttgatgcct ggcaggaagc gttccagcaa tctccctaca aagccccgcc
    12361 taaaactgcg gtctggttta ttaaaccgcg caatacggtg attggttgcg gtgaaccgat
    12421 tccctttcca cagggtgaaa aagtactgag cggtgcgact gttgcgctga ttgtggggaa
    12481 aacggcgacg aaagtacgtg aagaagatgc ggcagagtac atcgccggat atgcgctgga
    12541 taacgacgtc agcctgccgg aagagagctt ttaccgtccg gcaatcaaag caaaatgtcg
    12601 tgatggattc tgccccattg gcgaaaccgt ggctctcagc aatgtcgata atctgactat
    12661 ctataccgag atcaacgggc gtcctgccga tcactggaat accgccgatt tacaacgtaa
    12721 cgccgcacag ctgctgagcg ccctgagcga atttgccacg ctgaatccag gcgatgctat
    12781 tctgctcggc acgccacagg cgcgcgtgga aatacagcca agcgatcgcg tgcgtgttct
    12841 cgcagaaggt ttcccgccgc tggaaaatcc ggtagtggac gaacgtgaag tgaccacgcg
    12901 caagagcttc ccaacgctgc cacacccgca cggtacgctg tttgccctcg gcctgaacta
    12961 cgccgaccac gccagcgaac tggaatttaa gccaccggaa gagccgctgg tgttcctgaa
    13021 agcgccgaat accctcactg gcgacaacca gacctccgtg cgcccgaaca atattgaata
    13081 catgcactac gaagcggagc tggtggtagt tattggcaag caggcgcgta acgtcagcga
    13141 agccgatgcc atggattatg tcgcgggcta caccgtgtgt aacgactacg ccattcgcga
    13201 ctatctggaa aactactacc gccctaacct gcgggtcaaa agccgcgacg gactgacgcc
    13261 gatgctttca accatcgtgc cgaaagaggc gatcccggac ccgcataatc tgacccttcg
    13321 caccttcgtc aacggcgagt tacgccagca aggcaccacc gccgatctga tcttcagcgt
    13381 gcccttcctg atcgcctact taagcgaatt tatgaccctg aatccgggcg acatgatcgc
    13441 caccggcaca ccaaaaggct tatctgacgt ggtgcctggc gatgaagtag tggtggaagt
    13501 agaaggcgtg ggccgcctgg tgaaccgaat tgtgagtgag gaaacagcga aatgaaaaaa
    13561 gtaaatcatt ggatcaacgg caaaaatgtt gcaggtaacg actacttcca gactaccaat
    13621 ccggcaacgg gtgaagtgct ggcggatgtg gcctctggcg gtgaagcgga gatcaatcag
    13681 gcggtagcgg cagcgaaaga ggcgttcccg aaatgggctg atctgccgat gaaagagcgt
    13741 gcgcgcctga tgcgccgtct gggcgatctg atcgaccaga acgtgcctga gatcgccgcg
    13801 atggaaaccg cggacacggg cctgccgatc catcagacca aaaatgtgtt gatcccacgc
    13861 gcttctcaca actttgaatt tttcgcggaa gtctgccagc agatgaacgg caagacctat
    13921 ccggttgacg acaagatgct caactacacg ctggtgcaac cggtaggcgt ttgtgcgctg
    13981 gcgtcgccgt ggaacgtgcc gtttatgacc gccacctgga aggtcgcgcc gtgtctggcg
    14041 ctgggcaata ccgcggtgct gaagatgtcg gaactctccc cgctgaccgc cgaccgcctg
    14101 ggtgagctgg cgctggaagc tggtattccg gcgggcgtgc taaacgtggt acagggctac
    14161 ggcgcaaccg caggcgatgc gctggtccgt catcatgacg tgcgtgccgt gtcgttcacc
    14221 ggcggtaccg ccaccgggcg caacatcatg aaaaacgccg ggctgaaaaa atactccatg
    14281 gagctgggcg gtaaatcacc ggtgctgatt tttgaagatg ccgatattga gcgtgcgctg
    14341 gacgccgccc tgttcaccat cttctcaatc aacggcgaac gctgcaccgc cggttcgcgc
    14401 atctttattc agcagagcat ctatccagaa ttcgttaaac gctttgccga acgcgccaac
    14461 cgtctgcgcg tgggcgatcc gaacgatccg aatacccagg ttagggcgct tatcagtcag
    14521 caacactggg aaaaagtctc cggctatatc cgtctcggca ttgaagaagg cgcaaccctg
    14581 ttggcgggcg gcccggataa accgtccgac ctgcctgcgc acctgaaagg cggcaacttc
    14641 ctgcgcccaa cggtgctggc ggacgtagat aaccgtatgc gcgttgccca ggaagagatt
    14701 ttcgggccgg tcgcctgcct gctgccgttt aaagacgaag ccgaaggctt acgcctggca
    14761 aacgacgtgg agtacggcct cgcgtcgtac atctggacac aggatgtcag caaagtgtta
    14821 cgcctggcgc gtggcattga agcaggcatg gtgttcgtca acacccagaa cgtgcgcgac
    14881 ctgcgccagc catttggcgg cgtaaaagcc tccggcaccg ggcgtgaagg cggtgagtac
    14941 agcttcgaag tgttcgcgga aatgaagaac gtctgcattt ccatgggcga ccatccaatt
    15001 ccgaaatggg gagtctgata tgggtaagtt agcgttagcc gccaagatca cgcacgtacc
    15061 gtcgatgtat ctctctgaac taccaggaaa aaaccacggt tgtcgccagg gtgcgatcga
    15121 cgggcataaa gagatcagca agcgttgccg ggaaatgggc gtcgatacca ttatcgtttt
    15181 cgatactcac tggctggtca acagtgctta tcacatcaac tgtgcagacc attttgaagg
    15241 cgtctacacc agcaacgagc tgccgcattt tattcgcgac atgacctaca actacgaggg
    15301 caacccggag ttggggcagc taattgccga ggaagcctta aagctcggcg tgcgggcaaa
    15361 agcgcacaat attcccagcc tgaaactgga atacggcacg ctggtaccga tgcgctacat
    15421 gaatgaagac aagcacttca aagtggtctc catttcggct ttctgcacgg ttcacgattt
    15481 tgccgacagc cgcaagctgg gcgaagcgat tctgaaagcg atcgaacagt acgacggcac
    15541 cgtggcggtc cttgccagcg gttcgttatc gcaccgcttt attgacgatc agcgtgcgga
    15601 agaagggatg aacagctaca cccgcgagtt cgaccgccag atggacgagc gtgtggtgaa
    15661 gctgtggcgc gaaggccagt tcaaagagtt ttgcaatatg ctgccggagt acgccgacta
    15721 ctgctacggc gaaggcaata tgcacgacac ggtgatgctg ctggggatgc tcggctggga
    15781 taaatacgac ggcaaggtgg agtttattac cgagctgttc ccaagctctg gcaccggtca
    15841 ggttaacgct gttttcccgc tgcccgcgta aggacgtttt atgccgcact ttatcgtgga
    15901 atgtactgaa aatattcgtg aagaagcccg cttacctgag ctgtttgcca gtgttaacac
    15961 tgcactggcg gcaacgggga tttttccgct tgggggaata cgtagccggg cgcactggat
    16021 cgatacctgg caaatggctg atggcaagca cgattacgcc tttgtgcata tgacgctaaa
    16081 aatcggcagc ggacgcagtc tggaaagccg tcaggaagtg ggcgaaatgc tgtttgatct
    16141 gattaaaacg cacttcgcct cactgatgga gagccgttac ctagcgcttt catttgagat
    16201 tgcagagctg cacccgacgt tgaatttcaa acagaacaac gtacacgcgt tgtttaaata
    16261 aatttgcacc agcttccagg ccggatgata aggcgctgcc gcatccggca atgccacagg
    16321 atatcgctat gttcgataaa cacacccaca ccctgatcgc ccagcgtctg gatcaggcag
    16381 aaaaacagcg cgaacagatc cgcgcgatct cgctggatta cccggagatc accatcgaag
    16441 acgcctacgc cgtgcagcgc gaatgggttc gcctgaaaat cgccgaaagt cgcacgctga
    16501 aaggccacaa aatcggtctg acctcgaaag cgatgcaggc aagctcgcag atcagcgaac
    16561 cggattacgg tgcactgctg gacgacatgt tcttccacga tggcagcgat atcccgaccg
    16621 atcgctttat cgtgccgcgc attgaagtgg agctggcttt tgtgctggca aaaccgctgc
    16681 gtgggccaaa ctgcacgctg ttcgacgttt acaacgccac ggactatgtg atcccggcgc
    16741 tggagctgat cgacgctcgc tgccacaaca tcgatccgga aacccagcgc ccgcgcaaag
    16801 tattcgacac catttccgat aacgccgcca acgccggagt gatcctcgga ggtcgtccga
    16861 tcaaaccaga cgaactggat ctgcgttgga tctccgccct gatgtatcgc aatggcgtga
    16921 ttgaagaaac cggcgtcgcc gctggtgtgc tgaatcatcc ggcaaacggc gtggcctggc
    16981 tggcgaacaa actcgcaccg tatgacgtac aactggaaac cgggcaaatc attctcggtg
    17041 gttcgttcac cctcccggtt ccggcgcgta agggcgacac cttccacgtc gattacggca
    17101 acatgggctc cattagctgc cgctttgttt aaggagagaa cgatggaaaa cagttttaaa
    17161 gcggcgctga aagcaggccg tccgcagatt ggattatggc tggggctgag cagcagctac
    17221 agcgcggagt tactggccgg accaggattc gactggttat tgatcgacgg tgagcacgcg
    17281 ccgaataacg tgcaaaccgt gctcacccaa ctacaggcga ttgcgcccta ccccagccag
    17341 ccagtggtac gcccatcgtg gaacgatccg gtgcaaatca aacaactgct ggacgtaggc
    17401 acacaaacct tactggtgcc gatggtacaa aacgccgacg aagcccgtaa agcggtacgc
    17461 gccacccgtt atccccccgc cggtattcgc ggtgtgggca gtgcgctggc tcgcgcctcg
    17521 cgctggaatc gcattcctga ttacctgcaa aaagccaacg atcaaatgtg cgtgctggtg
    17581 cagatcgaaa cgcgtgaggc aatgaagaac ttaccgcaga ttctggacgt ggaaggggtc
    17641 gacggcgtgt ttatcggccc ggcggatctg agcgccgata tgggttatgc cggtaatccg
    17701 cagcacccgg aagtacaggc cgccattgag caggcgatcg tgcagatccg cgaagcgggc
    17761 aaagcgccgg ggatcctgat cgccaatgag caactggcaa aacgctatct ggaactgggc
    17821 gcgctgtttg tcgccgtcgg cgttgacacc accctgctcg cccgcgccgc cgaagcgctg
    17881 gcagcacggt ttggtaatag ttcttctatt tcgatggcga agcaaaacaa caactctgtc
    17941 tactaaacgt acgatgtgga gcgcatcatg acgacctcta ccctgcaata taaagataat
    18001 aaaactgttg aaattgaaca tcgtgtcatt aataaattat ttcgtcgcct aatcattttt
    18061 ctttttattc tgtttgtttt ttcgtttctt gatcgcatca atataggatt tgccggactg
    18121 acaatgggca aggatcttgg cctgacgtcc acaatgtttg gcctcgccgc aacactcttt
    18181 tatgtcacgt acgtcatgtg cgggatcccc agtaatatga tgttagcgaa aattggcgct
    18241 cgtcgatgga tcgctgggat catggtggtc tggggaattg cttccacctg cacaatgttt
    18301 gcaacaagcc ccgaaacact ctatctactg cgtatgctgg ttggtattgc tgaggcgggt
    18361 tttttacctg gtattttggt ttatctgacc tggtggttcc cggcatatca tcgggccaga
    18421 gccaatgcct tatttatgat agcgatgcca gtcactatga tgctgggatt gatcctttca
    18481 ggctacattc tggccttgga tggactgtgg aatctaaaag gctggcaatg gctctttttg
    18541 ctggaaggtc tgccatcggt cattcttggt gtcgtcacct ggttttatct caacgacaca
    18601 ccagaccagg cgacctggct tgatgaaaac gaaaaacagg ctttaaaaac gatgattctc
    18661 cgtgaacaag agagtgcatt tgcccatgca tctgttccac gctcaacctt acgtgaagtc
    18721 ctgaccccag ccatcatgct atatacactt gcttattttt gcccgactaa tactctgagt
    18781 gcgattaata tctggacgcc gcaaatccta cagagtttca atactggcag tagcaacatc
    18841 atgattggtt tgctggcggc catcccccaa ttctgcacaa tttttggcat gatctggtgg
    18901 agtcgtcgtt ccgaccgtct taaagaacgc aaaaagcata cgattcttcc ctacctgttt
    18961 gccgctgccg gatggttact cgcttctgcg acagatcaca gcctcattca actgagcagc
    19021 atcattatgg cctcaatggg atcatttacc gcgatgacta tcttttggac aacaccggat
    19081 caagttataa gcttgcaatc gcgtgccgtt gcgatggctg tcatcaatgc tgtcggcaat
    19141 gtaggttcag cagtcagtcc attacttatc ggtattttga aagatgcaac cggcagtttt
    19201 agctcaggtt tgtggtttgt agcaggattg ctggtactgg gcgcgttagt catgacccga
    19261 atccctatgg ggagtcggga acactctctt tcatctgaac ttatcgcacc aaaaccgcaa
    19321 taaggagtca tgatgtccag tgaagctatc tttgaacatg gttatgaaaa tattgatttg
    19381 gtccgggaat atgatgcccg ctacgctggt gaagatgtgc attatgaaac atttgcccgg
    19441 ttagcgaatt tctttggacg cgatatgcgc cctcactggc acgatagata ttttcaactc
    19501 cattatctgg tcactggacg cattacttta caactggatg agcacttcta ttctgtgcgc
    19561 gcaccgcttt ttgtactaac accaccatcg gtgccacata ctttctttac tgatcctgat
    19621 agcgatgggc atgttctgac cgtacgtcag gagttaattt ggcccctggt agaaaagctc
    19681 tggcctggta gtggagatgc cgcaatgcta caaggtatct gtctgtcact ggagggaatg
    19741 gaacatacac tggcggcatt aaatcattat tggccgataa tatccctgga gtttcaacaa
    19801 aataagcaag gacgggaaat tctattgcaa tcactggcgc aaagtatttt tacaatccta
    19861 ctgcgtgaag caccaccaaa tgatatttca acatgcagcg tgcgcggcga gatgtgttta
    19921 ttccagaagt ttaatcggct gattgacgaa aattatcgcc aacatttaat ggtccctgaa
    19981 tatgcaacaa tgctcggtat gagtgaatca cggcttaccg agctgtgccg acgttttgcc
    20041 aatcaatcgc cgaagagatt gattttcgaa cgagtgagcc gggaagccag acggcttctg
    20101 ctctatagcg agcaaagcat caaccagata gcccttgatc ttggctacaa agatcccgcc
    20161 tatttcgccc gttttttcaa ccgaatgatg ggttgctcac caacacaatt ccgtggtcgc
    20221 tgaccattta tcaacctgag agactcgaca tgaaaatatc tttacgctca ctatttgtgg
    20281 cgtgcctgat tgcgccagtg gcattcacct acgcctccga accgcaataa atgaaaattg
    20341 tactacttgg aacgaaaggt ggcccttctt tattaaaagc tgaccgttta ccgatggcaa
    20401 cggcacttat tattggcaat aaaacctggt taattgatgc tggttacggg gccagcttac
    20461 aactggtaaa aaataatatt ccactgcgcc agattgataa tattttactc acccatttac
    20521 acagtgatca tattctggac tatccatcag tcttaatgaa tgcatgggct agcggtctgc
    20581 aggacaaaac tattcatgta ttcggtccgc caggcacccg agccatgacc gctgccagtt
    20641 ggcaggtctt cgatcgggac atcacactgc gtatggaaga agaaggaaaa cccgatcctc
    20701 gcaaactcgt tacagctcaa gatgttggtg aaggcgtagt ttataaagat gataccgtga
    20761 ccgtgactgc attaaaggtc cctcattctc ctttccctga cggtgaggct tttgcttatc
    20821 gcttcgacac acaaggcaag cgaattgtct tctcaggaga tacctcgtac tttcccccac
    20881 tggcaacttt ctcagaaggc gctgatatac ttgtgcatga ggccgtacat gagccctccg
    20941 tcgctaagct ggcagacagt attggcaatg gtaaaacact ggcaaaagcc attgcttctc
    21001 accatacgac tattgaggat gtaggcaaaa ttgccaatga agcaaaagtg aaaaaactca
    21061 tccttagcca cctggtacca atcaccgttc cggatgaaac atggaagcaa gaagcacaga
    21121 aaactattca ggtcctatcg tcgttggtca tgaaaatatg acgattacca ttccattcgc
    21181 cccataaaaa attccgtccc cgttagccct tgttagcggg gaatattatg atttgcctta
    21241 ctgtaagttg atcacgatcg cagtttacta tttaactcca ccttgctgtc gaaaagtacc
    21301 aacgttcgcc tgaaacgtct cttcccgaca accgcgttca acgcttcaat taaataacaa
    21361 aaacaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
    21421 cagaagattt ccgcgccagt acccaacgtc ctttcaccgg ggaagagtat ctgaaaagcc
    21481 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc accactcatc
    21541 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
    21601 agatgcagga ctctctgtgt tggaacaccg acaccggcag cggcggctat acccataaat
    21661 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgcgacgcc atcgctgaat
    21721 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagcc gctttcggtt
    21781 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
    21841 atacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
    21901 tcatttgccg accgataaag tgaaagacgt ttacatcaag ctggaaaaag agactgacgc
    21961 cgggattatc gtcagcggtg cgaaagtggt tgccaccaac tcggcgctga ctcactacaa
    22021 catgattggc ttcggctcgg cacaagtgat gggcgaaaac ccggacttcg cactgatgtt
    22081 cgttgcgcca atggatgccg atggcgtgaa attaatctcc cgcgcctctt atgagatggt
    22141 cgcgggtgct accggctcgc catacgacta cccgctctcc agccgcttcg atgagaacga
    22201 tgcgattctg gtgatggata acgtactgat cccatgggaa aacgtgctga tctaccgcga
    22261 ttttgatcgc tgccgtcgct ggacgatgga aggcggtttc gcccgtatgt atccgctgca
    22321 agcctgtgtg cgcctggcag tgaaactcga cttcattacg gcactgctga aaaaatcact
    22381 cgaatgtacc ggcaccctgg agttccgtgg tgtgcaggcc gatctcggtg aagtggtggc
    22441 gtggcgcaac accttctggg cattgagtga ctcgatgtgt tcagaagcga cgccgtgggt
    22501 caacggggct tatttaccgg atcatgccgc actgcaaacc tatcgcgtac tggcaccaat
    22561 ggcctacgcg aagatcaaaa acattataga acgcaacgtt accagtggcc tgatctacct
    22621 cccttccagt gcccgtgacc tgaacaatcc gcagatcgac cagtatctgg cgaagtatgt
    22681 gcgcggttcg aacggtatgg atcacgtcca gcgcatcaag atcctcaaac tgatgtggga
    22741 cgccattggc agcgaatttg gtggtcgtca cgagctgtat gaaatcaact actccggtag
    22801 ccaggatgag attcgcctgc aatgtctgcg ccaggcacaa agctccggca atatggacaa
    22861 gatgatggcg atggttgatc gctgcctgtc ggaatacgac cagaacggct ggactgtgcc
    22921 gcacctgcac aacaacgacg atatcaacat gctggataag ctgctgaaat aacgcagcag
    22981 gaggttaaga tgcaattaga tgaacaacgc ctgcgctttc atgacgcgat ggccagcctg
    23041 tcggcagcgg taaatattat caccaccgag ggcgacgccg gacaatgcgg gattacggca
    23101 acggctgtct gctcggtcac ggatacacca ccgtcgctga tggtgtgcat taacgccaac
    23161 agtgcgatga acccggtttt tcagggcaac ggcaagttgt gcgtcaacgt cctcaaccat
    23221 gagcaggaac tgatggcgcg ccacttcgcg ggcatgacag gcatggcgat ggaagagcgt
    23281 tttagcctct catgctggca aaaaggtccg ctggcgcagc cggtgctaaa aggttcgctg
    23341 gccagtcttg aaggagagat ccgcgatgtg caggcgattg gcacacatct ggtgtatctg
    23401 gtggagatta aaaacatcat cctcagtgca gaaggtcacg gacttattta ctttaaacgc
    23461 cgtttccatc cggtgatgct ggaaatggaa gctgcgattt aagctccagc cccttcacgc
    23521 attcgtcgaa ggggcatttt tattgtgatc tcaatcacat atctacccga tgaataattc
    23581 ttgcccaacc gctcaccgcc attcctgacc gcttacatcc ctaaaataac cactcagtta
    23641 tttaccttac tttacgcgca cgtatctctg gcaacatcac tgcaggatag cggtcaattt
    23701 acctcctcaa acgcaacgca aacctagaac ggcttcggcc tatgattaac aaattactct
    23761 atggattttg gtttgcagga aggcagcaag tgagtgaatc cccgggagct tacaaaagta
    23821 agtgtcagag gtgaacgaac gcagctgccg cacctgtaag ccaaaagacg acgagtaaaa
    23881 tgccaggttt tactatggat acgaaaaaac tattcaagca cataccctgg gtgattctcg
    23941 gaatcatcgg tgcattctgc ctcgcggtag ttgcattacg tcggggggag cacgtcagcg
    24001 ccctgtggat cgtggtcgcc tctgtgtcgg tgtatctggt ggcgtatcgc tactacagtc
    24061 tgtacatcgc ccagaaggtg atgaaactcg accccacgcg cgcgacgcct gcggttatta
    24121 acaacgacgg tctgaactac gttccgacca accgttacgt gttgtttggt caccacttcg
    24181 ccgctatcgc cggtgctggt ccgctggtgg gaccggttct cgccgcgcag atgggctacc
    24241 tgcctggcac gctgtggctg ctggcggggg tagtgctggc cggtgcggta caggacttta
    24301 tggtgctgtt tatctcctct cgccgtaacg gcgcatctct cggtgagatg atcaaagaag
    24361 agatggggcc agtaccgggg actatcgcgc tgtttggctg tttcttaatc atgatcatca
    24421 tcctcgccgt tctggcgctg attgtggtta aagccctggc cgaaagtccg tggggtgtct
    24481 tcaccgtttg ctcaaccgta ccgattgcgc tgtttatggg tatctacatg cgctttatcc
    24541 gtccggggcg tgtgggtgaa gtctctgtta ttggtatcgt gctgctggtt gcctctatct
    24601 acttcggtgg cgtgattgct cacgatccgt actggggtcc ggcactgacc tttaaagaca
    24661 ccaccattac cttcgcgctg attggctatg cgtttgtttc cgcactgctg ccggtgtggc
    24721 tgatcctcgc accgcgcgac tatctggcaa ccttcctgaa aatcggcgtt atcgtcggcc
    24781 tggcgctggg tatcgtggtg ctgaacccgg aactgaaaat gcctgcgatg acccagtaca
    24841 ttgacggtac tggcccgctg tggaaaggcg ctctgttccc gttcctgttc atcaccatcg
    24901 cctgtggtgc ggtatctggc ttccacgcgc tgatctcttc cggtacgacg ccgaaactgc
    24961 tggctaacga aaccgacgcg cgtttcattg gctacggcgc aatgctgatg gagtccttcg
    25021 tggcgattat ggcgctggtt gcagcgtcca tcatcgaacc gggtctttac ttcgcgatga
    25081 acaccccgcc tgctggcctt ggcatcacca tgcctaacct gcatgaaatg ggtggcgaga
    25141 acgcgccgat catcatggcg cagctgaaag acgttaccgc acacgcggca gcgaccgtca
    25201 gctcctgggg cttcgtgatt tcgccagagc agatcctgca aaccgcgaaa gacattggtg
    25261 agccttctgt cctgaaccgt gcaggtggtg cgccgacgct ggcggtaggt atcgctcacg
    25321 tgttccacaa agtgctgccg atggctgaca tgggcttctg gtatcacttc ggtattctgt
    25381 tcgaagccct gttcatcctg accgcgctgg atgcgggtac ccgttctggc cgctttatgc
    25441 tgcaagacct gctgggtaac ttcatcccgt tcctgaaaaa aaccgactct ctggttgccg
    25501 gtatcatcgg tactgcgggc tgtgtgggtc tgtggggcta cctgctgtat cagggcgtgg
    25561 tcgatccgct gggcggcgtt aagagcctgt ggccgctgtt cggtatctcc aaccagatgc
    25621 tggcagccgt agcgctggta ctgggcaccg ttgtgctgat taagatgaag cgcacccaat
    25681 acatctgggt aactgttgtt ccggctgtat ggctgcttat ctgcaccacc tgggcgctgg
    25741 gtctgaaact gttcagcacc aacccgcaga tggaaggctt cttctacatg gcaagccagt
    25801 acaaagagaa gattgctaac ggtactgacc tgacggcgca gcagattgcc aacatgaacc
    25861 acatcgttgt gaacaactac accaacgcag gcctgagtat tctgttcctg attgtggtgt
    25921 acagcatcat cttctacggt ttcaaaacct ggcttgcggt gcgtaacagc gacaaacgta
    25981 ctgacaaaga aacaccgtac gttccaatcc cggaaggcgg cgtgaagatc tcttcgcacc
    26041 actaaccgtg tttagccccg cttcggcggg gctttgttct tatcagagtg aactatgttt
    26101 ggtaacttag gacaggcaaa aaaatatctc ggccaggcgg cgaagatgtt gattggtatt
    26161 ccagactacg acaactacgt cgagcatatg aagaccaacc atcccgacaa gccgtacatg
    26221 agctatgaag aattcttccg cgaacgccag aacgcgcgct acggcggcga tggtaaaggc
    26281 ggtatgcgct gttgttaatg gagagagcat gaacccgatt gcagttaccc tactcaccgg
    26341 ttttttaggc gcgggcaaaa ctaccctgct gcgccatatt cttaacgaac aacacggcta
    26401 caagattgcc gtgattgaaa acgaattcgg cgaagtctcc gttgatgacc aactgattgg
    26461 cgaccgcgcc acgcagatca aaacgctgac caacggctgc atctgctgtt cgcgctccaa
    26521 cgagctggag gacgcactac tcgacctgct ggataatctc gacaagggca atattcagtt
    26581 cgatcgtctg gtgattgaat gcaccggcat ggccgatccc ggcccgatta ttcagacctt
    26641 tttctcccat gagattttat gccagctcta cctgctggac ggcgtgattg cgctggtgga
    26701 tgcggtacat gccgatgagc agatgaacca gttcaccatc gcccagtccc aggtgggcta
    26761 cgccgaccgt attctgctga ctaaaaccga cgtcgcaggc gaagcagaga aactgcgtga
    26821 acgcctggcg cgcatcaacg cccgcgcacc ggtctacacc gtcacccacg gcgacatcga
    26881 cctgggcctg ctgttcaaca ccaacggttt tatgctggaa gagaacgtcg tcagcaccaa
    26941 accgcgtttc cactttatcg cggataaaca aaacgatatt tcgtcaattg tggtggaact
    27001 ggattacccg gtagatatca gcgaagtttc ccgcgtgatg gaaaacctgc tgctggaatc
    27061 ggcggataaa ctgctgcgtt acaaagggat gctgtggatt gacggcgaac ctaaccgcct
    27121 gctgttccag ggcgtccagc gcctctacag cgccgactgg gacaggccgt ggggcgatga
    27181 aaaaccgcat agcacaatgg tgtttatcgg gattcagttg ccggaggaag agattcgggc
    27241 ggcgtttgcg ggattgagga agtaagttcg ggaaagatca actttttgag agttccaccg
    27301 caacattatt acgagtggaa ctctttagat atgtcagacc aaccaaagct tcttcaattg
    27361 cgttttcatt ttttcatgtc tcattttcag ttcagccaat ccccattctg aagaattaca
    27421 aacatctttt gttaaataaa aagagctttt actattaagt ttctcataaa tagatttctt
    27481 tttgataaaa tcatagtgtt gtgtttcaga atttttggaa ccacttatga gtgttaaatt
    27541 acctatttta tgcagccagt agacatgctc ttgaggttgg aatctattaa tccagtattc
    27601 attaactaat gcttgaggaa gaatatgctc aattgtgatt ctccccgtat aggttttaat
    27661 cacgctctca tcttgttgct ccatatctaa gcgaagcaaa attgctttta ttaagttcac
    27721 ttgattcgga cgaggctcat ataaatcttc atccagagca gctataaaac ctgaattatc
    27781 tgcatgttgg tttatctgat ttatcacaga atcaaaaggc atatcattat ttattgctac
    27841 cagagcagaa taacaaacca tttctctttg gcttttaatt tgttttttta accacccatg
    27901 catatatacc ttctcaaagg cggtaataaa ttgagaaaaa tcatctaaat taaaatcttc
    27961 tgtacgagcc attcgattca tgaatgccat aaccggagga atccactcat ctacccctaa
    28021 attacttagc gatgctattt ttcgtcgtat tgaagggtgt tcaaaatcat tctctaaaat
    28081 cttagtata
//
LOCUS       DAZSNM010000027        27660 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZSNM010000027 DAZSNM010000000
VERSION     DAZSNM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27660
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.027"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..354
                     /locus_tag="ACHW42_001038"
     CDS             <1..354
                     /locus_tag="ACHW42_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR8942959.1"
                     /translation="SARRFTPPLRHSATAAPDPLVKFSGVTVDLAARVIHRGEEEVHL
                     TPIEFRLLAVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARP
                     RHFITETGIGYRFML"
     gene            516..650
                     /locus_tag="ACHW42_001039"
     CDS             516..650
                     /locus_tag="ACHW42_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR8942960.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTASGKGKWKIITA
                     "
     gene            629..733
                     /gene="speFL"
                     /locus_tag="ACHW42_001040"
     CDS             629..733
                     /gene="speFL"
                     /locus_tag="ACHW42_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR8942961.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            1044..3241
                     /gene="speF"
                     /locus_tag="ACHW42_001041"
                     /pseudo
     CDS             1044..3241
                     /gene="speF"
                     /locus_tag="ACHW42_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            3238..4557
                     /gene="potE"
                     /locus_tag="ACHW42_001042"
     CDS             3238..4557
                     /gene="potE"
                     /locus_tag="ACHW42_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR8942962.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4613..6253)
                     /gene="pgm"
                     /locus_tag="ACHW42_001043"
     CDS             complement(4613..6253)
                     /gene="pgm"
                     /locus_tag="ACHW42_001043"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR8942963.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(6279..6824)
                     /gene="seqA"
                     /locus_tag="ACHW42_001044"
     CDS             complement(6279..6824)
                     /gene="seqA"
                     /locus_tag="ACHW42_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR8942964.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            7009..7773
                     /gene="ybfF"
                     /locus_tag="ACHW42_001045"
     CDS             7009..7773
                     /gene="ybfF"
                     /locus_tag="ACHW42_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR8942965.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7913..8206
                     /gene="ybfE"
                     /locus_tag="ACHW42_001046"
     CDS             7913..8206
                     /gene="ybfE"
                     /locus_tag="ACHW42_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR8942966.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            8346..8876
                     /gene="fldA"
                     /locus_tag="ACHW42_001047"
     CDS             8346..8876
                     /gene="fldA"
                     /locus_tag="ACHW42_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR8942967.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            9086..9172
                     /gene="uof"
                     /locus_tag="ACHW42_001048"
     CDS             9086..9172
                     /gene="uof"
                     /locus_tag="ACHW42_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR8942968.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            9165..9611
                     /gene="fur"
                     /locus_tag="ACHW42_001049"
     CDS             9165..9611
                     /gene="fur"
                     /locus_tag="ACHW42_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR8942969.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9695..10021)
                     /gene="chiQ"
                     /locus_tag="ACHW42_001050"
     CDS             complement(9695..10021)
                     /gene="chiQ"
                     /locus_tag="ACHW42_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR8942970.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(10058..11464)
                     /gene="chiP"
                     /locus_tag="ACHW42_001051"
     CDS             complement(10058..11464)
                     /gene="chiP"
                     /locus_tag="ACHW42_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFR8942971.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(12043..13707)
                     /gene="glnS"
                     /locus_tag="ACHW42_001052"
     CDS             complement(12043..13707)
                     /gene="glnS"
                     /locus_tag="ACHW42_001052"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR8942972.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13910..15856)
                     /gene="nagE"
                     /locus_tag="ACHW42_001053"
     CDS             complement(13910..15856)
                     /gene="nagE"
                     /locus_tag="ACHW42_001053"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR8942973.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            16189..16989
                     /gene="nagB"
                     /locus_tag="ACHW42_001054"
     CDS             16189..16989
                     /gene="nagB"
                     /locus_tag="ACHW42_001054"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR8942974.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            17049..18197
                     /gene="nagA"
                     /locus_tag="ACHW42_001055"
     CDS             17049..18197
                     /gene="nagA"
                     /locus_tag="ACHW42_001055"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR8942975.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            18206..19426
                     /gene="nagC"
                     /locus_tag="ACHW42_001056"
                     /gene_synonym="nagR"
     CDS             18206..19426
                     /gene="nagC"
                     /locus_tag="ACHW42_001056"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR8942976.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            19474..20226
                     /gene="nagD"
                     /locus_tag="ACHW42_001057"
                     /pseudo
     CDS             19474..20226
                     /gene="nagD"
                     /locus_tag="ACHW42_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            complement(20277..20375)
                     /locus_tag="ACHW42_001058"
                     /pseudo
     CDS             complement(20277..20375)
                     /locus_tag="ACHW42_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20623..22287
                     /gene="asnB"
                     /locus_tag="ACHW42_001059"
     CDS             20623..22287
                     /gene="asnB"
                     /locus_tag="ACHW42_001059"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR8942977.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            22666..22742
                     /locus_tag="ACHW42_001060"
     tRNA            22666..22742
                     /locus_tag="ACHW42_001060"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22700..22702,aa:Met,seq:cat)
     gene            22751..22835
                     /locus_tag="ACHW42_001061"
     tRNA            22751..22835
                     /locus_tag="ACHW42_001061"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22785..22787,aa:Leu,seq:tag)
     gene            22859..22933
                     /locus_tag="ACHW42_001062"
     tRNA            22859..22933
                     /locus_tag="ACHW42_001062"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22891..22893,aa:Gln,seq:ttg)
     gene            22968..23042
                     /locus_tag="ACHW42_001063"
     tRNA            22968..23042
                     /locus_tag="ACHW42_001063"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23000..23002,aa:Gln,seq:ttg)
     gene            23058..23134
                     /locus_tag="ACHW42_001064"
     tRNA            23058..23134
                     /locus_tag="ACHW42_001064"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23092..23094,aa:Met,seq:cat)
     gene            23183..23257
                     /locus_tag="ACHW42_001065"
     tRNA            23183..23257
                     /locus_tag="ACHW42_001065"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23215..23217,aa:Gln,seq:ctg)
     gene            23295..23369
                     /locus_tag="ACHW42_001066"
     tRNA            23295..23369
                     /locus_tag="ACHW42_001066"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23327..23329,aa:Gln,seq:ctg)
     gene            complement(23523..24698)
                     /gene="ubiF"
                     /locus_tag="ACHW42_001067"
     CDS             complement(23523..24698)
                     /gene="ubiF"
                     /locus_tag="ACHW42_001067"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR8942978.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            24844..26268
                     /gene="miaB"
                     /locus_tag="ACHW42_001068"
     CDS             24844..26268
                     /gene="miaB"
                     /locus_tag="ACHW42_001068"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR8942979.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            26421..27461
                     /locus_tag="ACHW42_001069"
     CDS             26421..27461
                     /locus_tag="ACHW42_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR8942980.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            27458..>27660
                     /locus_tag="ACHW42_001070"
     CDS             27458..>27660
                     /locus_tag="ACHW42_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNAse YbeY"
                     /protein_id="HFR8942981.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVL"
BASE COUNT         6801 a         7078 c         6971 g         6810 t
ORIGIN      
        1 tctgcgcgtc gctttacgcc gccactccgc cactccgcca ccgccgcgcc cgatccgctg
       61 gtgaaatttt ccggtgttac cgtcgattta gccgcccgcg tgattcaccg gggtgaggaa
      121 gaggtgcatc tcacaccaat tgagttccgc ctgctggcgg tgctgctcaa caatgccgga
      181 aaagtactca cccagcgcca gctccttaac caggtgtggg ggccaaacgc ggtcgaacac
      241 agtcactatt tgcgtattta tatgggacat ctgcgacaaa aactggaaca ggatccggcc
      301 cggcctcgcc acttcattac tgaaaccggt attgggtatc ggtttatgct ttgaattgtt
      361 attcaataaa ttcgcttaaa tagtgattaa tttattatta aacctgcgcc gaatattatt
      421 atttggcagg tttaatttct taacgcaaat ataaaacacg aaattacaac atttaagtaa
      481 caaaacagca tctaatatgg cgatattcat aattaatgat ttattatctg gggtatgatc
      541 gttttttgtt gatcttcttc acagattata gccatttcat ggatagaata actctacctt
      601 caactgacac agcaagcggt aaaggtaaat ggaaaataat aaccgcttaa tgcctcatat
      661 aaggcggaca acccatatca tgaagtttgc ccatcgtaat agttttgact ttcatttctt
      721 taatgcccgc tagtcttctg actaaagggc accccaacgt acaggtctcc ctgactttaa
      781 gcattacagg ttaatacctg tattcctcgg tgctcatata ctgctaaccc ttttaaactc
      841 taaataattc gagtcgcagc acttgcaact tgaggtatga cgagtatagc cagttaccgg
      901 gctggtctgg gttattgcat ctgcaaaaag caaactactg atttatttat cagcggtgga
      961 gctttgcttt ttttccgacg tgatcgattt ctcctttgag aaattgagga cctgctatta
     1021 cctgaaataa agagatgaat aaaatgtcag aattaaaaat tgccgttagc cgttcttgcc
     1081 cggattgttt ttccactcat cgtgcatgcg tgaatattga cgaaagtaat tatattgacg
     1141 ttgccgccat tattttatca gtcaatgatg ttgaacgtgg aaaactcgat aaaatagacg
     1201 ctactggcta tggcattcct gtttttattg caacggaaaa tgaagaacgt gtaccagcag
     1261 aatatctgcc acgtatttct ggtgtcttcg agcattgcga atcacggaaa gagttttatg
     1321 gacgtcagtt agaaaccgct gctagccatt atgaaacgca actgcgcccg cccttcttcc
     1381 gcgcactggt cgattatgtc aatcagggca atagcgcttt tgactgcccg ggtcatcagg
     1441 gaggtgagtt tttccgtcgt catccggcag ggaatcagtt cgtggagtac ttcggagaaa
     1501 cgctgttccg ttccgactta tgcaacgcag acgtagcaat gggcgatctg ctgattcacg
     1561 aaggcgcgcc atgcattgca cagcaacatg cggcaaaagt gtttaatgcc gataaaacat
     1621 acttcgttct aaatggcact tcatcttcta acaaagtggt tttaaacgcc ctgctaacac
     1681 cgggtgatct ggtgctgttt gatcgcaata accacaaatc taaccaccac ggagcgttgc
     1741 tacaggctgg tgcaacaccg gtttatctgg aaacggcacg taacccgtat ggctttatcg
     1801 gtggcattga tgcgcactgt tttgaagaaa gttacctgcg tgagctgatc gcggaagtcg
     1861 caccgcagcg ggcaaaagag gctcgtcctt tccgcctcgc tgtgattcag ttaggcacct
     1921 acgacggtac gatttataac gcccgccagg tggtggataa aattggtcat ctgtgtgact
     1981 acattctgtt tgactccgca tgggttggct atgagcaatt tatcccgatg atggctgact
     2041 gttcgccgct gttgctggat cttaatgaga acgatccggg tattctagtt acgcaatccg
     2101 tacataaaca gcaggctggt ttttctcaaa cttcacaaat ccataaaaaa gacagccaca
     2161 tcaaagggca acagcgttat gtaccgcaca aacgcatgaa caacgccttt atgatgcacg
     2221 cctccaccag cccgttctat ccgctgtttg ccgcactgga tatcaacgcc aaaatgcatg
     2281 aaggtgtcag cggtcgtaat atgtggatgg attgtgtggt aaatggcatt aatgcccgca
     2341 aactgatcct cgataactgt cagcatattc gtccgttcgt acctgaactg gtggatggta
     2401 aaccctggca gtcgtatgaa acagcgcaaa ttgcggttga tctgcgcttc ttccagtttg
     2461 taccagggga acactggcat tcttttgaag gctatgcaga gaatcaatac tttgtcgatc
     2521 cttgcaaact gttgctgaca accccaggta ttgatgcacg taacggcgaa tatgaagcgt
     2581 tcggtgtacc cgcgacgatt cttgctaact tcctgcgcga aaatggcgta gtgccggaaa
     2641 aatgcgatct taacgccatc ctcttcctgc tgactccggc agaagatatg gccaaacttc
     2701 agcaacttgt gccctgctgg tacgcttcga aaaactgctt gagtccgacg cgccattagc
     2761 agaagtgctg ccttccatct acaaacagca tgaagagcgc tacgccggtt ataccctgcg
     2821 tcagttgtgc caggaaatgc atgatttgta tgcccgccac aacgtgaaac aactgcaaaa
     2881 agagatgttc cgcaaagcgc acttcccaca cgtcagcatg aatccgcaag aagccaacta
     2941 cgcctattta cgcggtgaag tggagctggt tcgtctaccg gatgccgaag gtcgtatcgc
     3001 tgccgaaggg gcgcttcctt atcctccggg tgtgctgtgt gttgttccgg gtgaaatctg
     3061 gggtggtgct gttttgcgtt acttcagcgc tctggaagaa gggatcaacc tgctgccagg
     3121 ttttgcaccg gagctgcagg gtgtctatat cgaagaacat gatggtcgta agcaagtttg
     3181 gtgctatgtc atcaagcctc gtgatgcgca aagcaccctg ttgaaagggg aaaaattatg
     3241 agtcaggcta aatcgaacaa aatgggcgtc gttcagttaa ccatactgac gatggtcaac
     3301 atgatgggct ccggtatcat catgctgccg acaaagcttg ccgaagttgg gacaatttca
     3361 attatctcct ggctggtgac agccgtaggc tcaatggcac tggcatgggc attcgcaaaa
     3421 tgcggtatgt tcagccgtaa atcaggtggt atgggcggtt atgccgagta tgctttcggt
     3481 aaatccggta actttattgc gaactatacc tacggcgtct cactgctgat tgctaacgtc
     3541 gcgattgcta tttcggcggt tggttacggc accgaactgc tcggcgcgag tttgtcgcca
     3601 gtgcagattg gtcttgcgac catcggggtg ctgtggattt gtaccgtggc taactttggt
     3661 ggtgcgcgca ttaccgggca aatcagtagc attaccgtgt gggggggcat tattccggtg
     3721 gtaggcctgt gcattatcgg ctggttctgg tttagcccga cgctttacgt tgattcctgg
     3781 aatccgcatc atgcaccgtt cttcagtgca gtaggttctt ccatcgctat gacgctgtgg
     3841 gcttttcttg gtctggagtc tgcgtgtgcg aatactgatg tagtggaaaa cccggaacgt
     3901 aatgtgccaa tcgcggtact cggcggtacg ttaggtgcgg cggtgattta tatcgtctcc
     3961 accaacgtga ttgcagggat tgtgcctaat atggagctgg caaattcaac ggcaccattt
     4021 ggcctggcct tcgcacagat gtttacacca gaagtgggta aagtcattat ggcgctgatg
     4081 gtgatgtcct gctgcggttc gctgcttggc tggcagttca ccattgccca ggtgtttaaa
     4141 tcttcatctg atgaaggcta cttccctaaa attttctccc gtgtaaccaa agtggatgca
     4201 ccggtgcagg gaatgttgac cattgtgatt atccagagcg ggttggcgct gatgacgatt
     4261 agcccgtcgc taaacagtca gttcaacgtg ctggttaacc tggccgtggt gaccaatatc
     4321 attccgtata ttctgtcaat ggcggcatta gtcattattc agaaggtcgc taatgtgccc
     4381 ccctcaaaag cgaaagttgc aaactttgtt gcttttgttg gcgcgatgta tagcttttat
     4441 gcgctgtact catccgggga agaagccatg ctgtacggtt ccatcgtgac cttccttggt
     4501 tggacactgt atggtctggt atcaccacgc tttgaactga aaaataaaca cggttaaaaa
     4561 agcaaagtaa aaaagggcga tcttgcgacc gctctttttt tattaaatgt gtttacgcgt
     4621 ttttcagaac ttcgctaaca atctcaaccg cttctttctc aatctgcttg cgatgttctt
     4681 caccgaggaa gctttcgcag tagatcttat atgcgtcttc cgtgcctgac ggacgcgcgg
     4741 cgaaccagcc gttgtcagtc atcactttca gaccgccaat agaagcaccg ttgcccggag
     4801 cagcagtcag gcgcgcggtg atcgggtcac ctgccagggt gctggcgctc accatttccg
     4861 gagacagctt agacagcgcc gctttttgtg cggaagtcgc agctgcctgc aaacggttgt
     4921 agctcggcgc accaaagcgt tttgccagtt cgttgtagtg ttcctgcggg ttcttaccgg
     4981 tgacagcggt gatttccgcc gccagcagac acataatgat gccgtctttg tcggtggacc
     5041 acggcgtgcc gtcgaaacgc aggaaggaag cccctgcgct ctcttcgccg ccaaagccga
     5101 agctgccgtc gaacagacca tcaacaaacc atttgaaacc taccggtact tctaccagct
     5161 tacggcccaa gtcattgacc acacggtcga tcatcgcaga agaaaccagc gttttaccga
     5221 cagcaacatc tttgccccac tgcggacgat gctggaacag gtagttgatc gccaccgcca
     5281 ggtagtggtt cggattcatc aaacctgccg gagtgacgat accgtgacgg tcataatccg
     5341 ggtcgttagc aaacgccaga tcgaacttat cacgcagtgc cagcagaccc gccatcgcac
     5401 actcggagga gcagtccata cggatcgcac cgtctttatc gaggtgcata aagcggaagg
     5461 tttgatcgac ctgatcgtta acgatggtca ggttgaggtt gtaatactcg ccaatgcgct
     5521 tccagtattc gataccggaa ccgcccaacg gatcaacgcc cagcgtcagg cccgctttct
     5581 gaatcgccgc catatcaacg atatcggcca gcccttccac gaacggctgc accagatcct
     5641 gctctttcac atagccggat gccatcgctt cgtcgaggga gatacgcttc acgcctttca
     5701 ggccatcggc catcagtgcg ttggccttgt cttccaccac tttggtgacg ttagtatcag
     5761 ccgggccacc atttggcgga ttgtacttga taccaccatc ttccggcggg ttatgggacg
     5821 gtgtaatcac gataccgtct gccagcgggc cacctttttt attgtgaacc aggatggcat
     5881 tggaaacggc aggcgtcgga gtgaagccat tgttttcctg cacaatgaca tcaacgccgt
     5941 tcgctgccag cacttccagt acggaaatga acgcaggctc ggacagggcg tgagtatctt
     6001 tacccacata gcaagggcca gtgatgccgt ttttcgcacg ttcttcagca attgcctgag
     6061 cgatcgccag aatgtgcggc tcgttaaagc tgtggcgcgc tgcactgcca cggtgaccgg
     6121 aagtaccgaa tttcaccgcg tgctccgcat tccctgcttc tggtttcagt acataatatt
     6181 gcgccgtcag ttgggcgacg ttaatcaaat cactctgttg tgcaggttgg cctgcacgat
     6241 tgtggattgc cattgctttg tcctttgtct gcaacgtttt agatagttcc gcaaaccttc
     6301 tcaatcaatt ccgccgggaa ttgcatcgac tgcatgatgt gttcgatcat gctgcattta
     6361 cggccggtgt tggtgttggt gatcacccaa tacggcgtgc ctggcacatg tttcggcttg
     6421 gtctgattac catttttcag cagcgtttgt tcatctgccg caaagtacac gcgtgtacga
     6481 ccgtgcaacg attccgttgc ttcggcaaac gcctgggcgt caagagaata tagtgtagac
     6541 aacagcagca taaagcgatt gaccgctcgc ttttgctctg cgtattcgtc cgaaagcaga
     6601 agttcacgca ttgcgcgaac cttgtcttta atcgttttga ccggcttcgc ttcgacgata
     6661 gcaggtgacg caacgcgaac ctctttcgtc accggagcag caggctgtga tgcggcggaa
     6721 aatttcaaca tacgccgtaa aatgtcggat gcgctctcgc cgatatgctt agtgtggctg
     6781 gcaatatagc tgtagagttc atcatcaact tcaatcgttt tcatcttaat ccagtgcagt
     6841 gtcttagctg aatacaagtc gccaagagta tatgaataaa tcccggcagc ggatagcgtc
     6901 aagcctggct tgcagaaaaa ctcggaataa accttatttg ttgcagccct gtggcacaat
     6961 ggtcaacata ataccctaac ccgacaacgc gaaaaaaaga actttgccat gaaattaaat
     7021 atccgcgcgc aaactgcaca aaaccagcac aataattctc ccatcgttct tgtccatggt
     7081 ctgtttggta gcctcgacaa ccttggcgta ctggctcgcg atctggtaaa cgatcacaat
     7141 atcatccagg ttgatatgcg taaccacggt ctttcaccga gagagccggt aatgaattac
     7201 ccggcgatgg cgcaggatct tgttgatacc ctggatgcac tgcagatcga caaagcaaca
     7261 tttatcggtc actccatggg cggtaaagcg gtaatggcac ttactgcact ggcccccgat
     7321 cgcatcgata aactggtggc gatcgatatc gcaccggtcg actatcacgt acgccgtcat
     7381 gatgagattt tcgcagctat caacgcggtc agtgaatcgg acgcacaaac tcgccagcaa
     7441 gcagcagcaa taatgcgcca gcatcttaat gaagaagggg tgattcagtt tctgttgaaa
     7501 tcttttgttg acggggagtg gcgctttaac gtgccggtat tgtgggatca gtatccgcat
     7561 attgtgggtt gggagaaaat cccggcatgg gatcaccctg ccctgtttat ccctggcggc
     7621 aattctccgt atgttagcga gcagtaccgt gatgatttac tggcacaatt tccacaggca
     7681 cgggcgcatg tgattgcagg cgcgggtcac tgggtccatg ctgaaaaacc ggatgcggta
     7741 ttacgcgcta tccgtcgcta tctcaatgat taacgcaact gattaaaaac ccagcgtccg
     7801 cgtcgctttc cgcgcgtggg cgttggcgtg actcatcgcg ctgatgtatt atggcgcgct
     7861 atccatccgg gcagaggcct ggctgattgt ttcccccgaa gtcaccaaga tcatggccaa
     7921 agaacaaacg gaccgtacga cattagatct gttcgcgcac gagcgtcgac cgggacgacc
     7981 gaaaactaat ccgctttcgc gcgatgaaca gctgcgtatt aataaacgca accagctaaa
     8041 acgcgacaaa gtacgtggcc ttaagcgtgt cgaactgaag ctgaacgcgg aagctgtcga
     8101 ggcgctgaac gagctggcgg aatcgcgtaa tatgagccgt agcgaactga tcgaagagat
     8161 gttaatgcag caactggcgg cgctgcgtag tcagggcatc gtttaaattt ccactttcat
     8221 gtagcacagt gtgcagtcct gctcgtttgc tgattccgca agactgcctg ttctgctatg
     8281 attgccttta tccgtgggca attttccacc cccatttcaa taagtttcaa gaggttattt
     8341 cactcatggc tatcactggc atctttttcg gcagcgacac cggtaatacc gaaaatatcg
     8401 caaaaatgat tcaaaaacag cttggtaaag acgttgccga tgtccatgac attgcaaaaa
     8461 gcagcaaaga agatctggaa gcttacgaca ttctgctgct gggcatccca acctggtatt
     8521 acggcgaagc gcagtgtgac tgggatgact tcttcccgac tctcgaagag attgatttca
     8581 atggtaaact ggttgcgctg tttggttgtg gtgaccagga agattacgcc gaatatttct
     8641 gcgacgcatt gggcaccatc cgcgacatca ttgaaccgcg cggtgcaacc atcgttggtc
     8701 actggccaac tgcgggctat catttcgaag catcaaaagg tctggcagat gacgaccact
     8761 ttgtcggtct ggctatcgac gaagaccgtc agccggaact gaccgctgaa cgtgtagaaa
     8821 aatgggttaa acagatttct gaagagttgc atctcgacga aattcttaat gcctgatgtg
     8881 atgcggcgta gactcatgtc tacgccgtat taatagataa tgccaatcaa aataattgct
     8941 acaaatttgt aacttttgct gttgtaccta tacaatgtcc cagtgttcaa gtggccttgc
     9001 cgttgtaaat gtaagctgtg ccacgttttt attaacaata tttgccaggg acttgtggtt
     9061 ttcatttagg cgtggcaatt ctataatgat acgcattatc tcaagagcaa attctgtcac
     9121 ttcttctaat gaagtgaacc gcttagtaac aggacagatt ccgcatgact gataacaata
     9181 ccgccctaaa gaaagctggc ctgaaagtaa cgcttcctcg tttaaaaatc ctggaagttc
     9241 ttcaggagcc ggacaaccat cacgtcagtg cggaagattt atacaaacgt ctgatcgata
     9301 tgggtgaaga aattggtctg gctacggtat atcgcgtact gaaccagttt gacgacgctg
     9361 gtatcgtcac ccgccacaat tttgaaggcg gtaaatccgt atttgaactg acacagcaac
     9421 atcaccacga tcacctgatt tgcctcgact gcggcaaggt tatcgaattt agtgatgatt
     9481 ccatcgaagc gcgtcagcgt gaaattgccg caaaacatgg cattcgcctg actaaccaca
     9541 gtctctatct ttacggtcac tgtgccgaag gcgattgccg cgaagatgag cacgcgcacg
     9601 aaggcaaata agccagcctg aacgagaaaa gccaacctgc gggttggctt ttttatacaa
     9661 gggaaataag aaaagcgaga gttacagctc tcacttattt gttattactg cgtatttcct
     9721 gccagacctt atcacaatcg gctttcaccg cctgatcatt tccggtttgc gttgcacgca
     9781 gacactgctg ataatccagt acgcgtacac tttcctggtc ggcaaactgc tgatgttgtt
     9841 tctctttctt cagcacgttt aacacgctct ggcaggcttc aattttttct ggcgaacctt
     9901 gcgcggtgtt gatacaggcg ctgtatgcct ctttcagacg gctgtcttcc tgtggtgcgg
     9961 ttgattgcgc acaagctacc agccccgatg ccatcatggc gatgagaatc agttttttca
    10021 ttgaatgcgg caggcacaaa acctgccgcg tcgggcatca gaagatggtg aatggtgcga
    10081 ttaccataaa tttcacgtca cgctcatcct ggaagatgtt accgtaaccg ccgccccagc
    10141 ttgggatgtc ggagtggttg tcgtattcgg tgaagtgcag tttgaacatc gtgcctttgg
    10201 cgcgaccgtc ctgaatggtg tagaccgcat ccaggctgta ggcagactct tcaatagtcc
    10261 ggtttttgtc gtagtacgca tccggattgc tctgccaggt cgcaggttta gcatcccatg
    10321 cgtaaacgta ggaagcgccg atggcgaagc ctggaagatt ccagtttttc aggtcataca
    10381 tcgcactgaa gaagaccgct ttttcgccgt tagcgttgaa gtcggaacgg ttatcccacc
    10441 agatatccag gcggccgttg gaggaagcgt aggttggggt catacgttgc aggaagtatc
    10501 cctgctgact gtcagcctta atccaggtgc cttcgaggcg caaatccact acgtcagccg
    10561 cccggtaacc aaaggtcaac gcttgcagcc aggcggtgcc gtcataaaga tcgttgacgc
    10621 tgcgatcgtc aactttatcg cgggtaccat agaactggta gctggtggtt aacgggctac
    10681 cggcgatatc aaatttgtag ctggctttgg caaaatattg atcgatatac ccttccgcct
    10741 gaccaaatgc cgcttccagt acgaagttat ttttgaagtc gtatttcgca ccgatggagt
    10801 gcagataatc aactttggtg gttttatcgt tctgataaaa ctcatccatt tccagatgcc
    10861 acggcgcttt gtattcgttg gtccacatgt aggagaaact caaggcacca gcatcgccgt
    10921 aatcaaaatt cgccccggct tccgccccct gataagtacc cggcataaag ctccagtgcg
    10981 gcgctaacag cgtttgaccg gttggctgaa tgtaacctgc cctcgcccaa accggaccgt
    11041 atttaaattt ggccgcagct ttatacaggc ttataccgct tttgtcgccg gaccagtctt
    11101 cgtcataggc tttattactt tttgaaaacg cgatttcgtt cgggtgggag ctgtcgccgt
    11161 tttccgccat ttcaatcgcc gtaaacgcag caatatcaag gccgaacata tcagcagcat
    11221 aaccggactg aaaatcgagg ttggcattcc aggtggagtg agaaaggttg gttttgtatt
    11281 tgtcgccgtc ggtaacatct ttacggtcgc gttcacgctg ccagtaatag ataccgccgg
    11341 ttaaggttga atcgtcgatg aatccttcgg cgcgagcctc cggcattgcc ataaagcccg
    11401 acattgctgt aacaccggca atagccagcg ccagcgtact acgtttgcca ctaaacgtac
    11461 gcatgggtta atcctctttg acgtataaat tgctgcacca aaggtgaaca gcgaaaaaaa
    11521 ataaaaatta aaaaagtagc gttgagaaaa gtctcgctga ctacaggaac agactatttt
    11581 tcgcgacgcg aattatcaat ctctttctgt aaccagaatg taagaacatg agcaaccgca
    11641 catattttga tgatttttgt tacaaggctt aagtttgcgg ttgaaaggct tgcagaagaa
    11701 taaaaacgtg aaattccagc aagatgaaag cggttacatt ttatttaatt atgttttgtg
    11761 attttaaatt tattttatgg ataaattgcg aagcggaatt aattcgcgaa gaaaagcaga
    11821 aaaaacgccg cagagcgcgg cgtttgggat tgtttgctat ttcttgccgg atgcggcgtg
    11881 aacgccttat ccggcctaca aaagcacgca aattcaatat attgcagaga tcatgtaggc
    11941 ctgataagcg aagcgcatca ggcaatttag cgtttgaaat ttattgtcta tttattgccg
    12001 gatgcgacgt aaatgcctca cccggcttgg tagaacttaa aattactcgc ctactttcgc
    12061 ccaggtatca cgcagcccaa cggtgcggtt aaataccggt ttttcggccg tggaatggcg
    12121 gctgtcgagg cagaagtaac cttcacgctc aaactggaat gctttacccg caaccgcatc
    12181 tttcagcgac ggttcagcaa agccctgttt gatcaccagc gattccgggt taatcaccga
    12241 caggaaatca tccgcagcac ctgggttagg cacgctgaac agacgatcat acaaacggat
    12301 ttcaaccggc agcgcatgtg ccgcgctcac ccagtgaata acacctttga ctttacgacc
    12361 atctgccgga tctttgctta aggtgtcggc gtcataagta cagaagatgg tggtgatatt
    12421 accttcggca tctttctcca cgcgttcagc cttaatgacg taagcattac gcagacgcac
    12481 ttctttaccc agcaccagac gtttgtactg cttgttagct tcttcgcgga aatcggcgcg
    12541 atcgatccaa atttcaccgc taaacggcac ctgacggctc cccatttccg gtttgttcgg
    12601 atggttcggc atggtgacca tttcgccttc gccctgatag ttttcgataa ccagtttcac
    12661 cggatcgata accgccattg cgcgcggcgc attttcgttg agatcttcac ggatgcagga
    12721 ttccagcgac gccatctcaa tggtgttgtc ctgcttggtc acgccgatgc gtttgcagaa
    12781 ctcacgaata gaagccgcag tgtaaccacg acgacgcaga ccggaaatgg tcggcatacg
    12841 cgggtcatcc cagccttcaa cgtgcttgtc ggtcaccagc aggttcagct tacgcttgga
    12901 catcacagtg tattccagat tcaggcgcga gaattcgtac tggcgcggat gaacaggaat
    12961 ggtgatgtta tccagtaccc agtcgtacag acgacggttg tcctggaact caagcgtaca
    13021 cagagagtgc gtaatacctt ccagcgcatc gctgatgcaa tgggtgaagt cgtacatcgg
    13081 gtagatgcac cacttgttgc cagtctggtg gtgttcagca aatttaatgc ggtacagcac
    13141 cggatcgcgc attacgataa atggcgacgc catgtcgatt ttcgcacgca ggcaggcttt
    13201 accttcttca aaaccaccgg cacgcatttt ttcgaacagc gccaagttct cttcaacgct
    13261 gcggtcgcgg tacgggctgt ttttacccgg ctgcgtcagg gtaccgcggt attcgcggat
    13321 ctgttccggt gtcagttcat caacgtacgc caggccttta ttgatcagtt cgatcgcata
    13381 ggcgtggagc tgatcaaaat aatcggagga gtaacggacg ttaccagacc agtgaaaacc
    13441 taaccactct acgtcgtttt tgatcgaatc gacatactcg atatcttctt ttaccgggtt
    13501 agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc tgggcgatcc cgaagttcag
    13561 gcagatagat ttcgcatggc caatatgcag atagccattc ggctccggcg ggaaacgggt
    13621 gtgtactgtg atgtgcttac cactggccag atcttcatcg atgatctgac ggataaagtt
    13681 agtcgggcgg gcttctgcct cactcatcgt ggattcctca aagcgtaaac aacgtataac
    13741 ggcgtatgat cttataagcg ggacaggctg acaactgtta gttttttaaa attgataatc
    13801 taacaaggat tatggggatg attgatgaaa aaaaaagcgg cagagaagat ctccaccgct
    13861 tgtcattgtt ggatgcgacg ctgccgtgtc gcatcaggca taaagcagat tactttttga
    13921 tttcatacag cggtgtttga cccgccacaa catggccctg agctttaatg agcaagccac
    13981 tgaaatcgtc gatattgctg caaaccaccg ggctaatcat cgagcgggcg ttagcgttca
    14041 ggtaatccag atccatttcc agaatcggtt gccctgcgct tacctgcgca ccctcttcca
    14101 ccagacgttt aaagccttta ccttccagcg ctacggtgtc gatacccata tggacgacga
    14161 tctccgcgcc tttttcggtt tccaggcaga acgcgtggtt ggtgttgaag attttcacga
    14221 ttgtccctgc ggctggtgat acgacgattt tatctgtcgg tttcaccgcc acaccgtcac
    14281 ccaccgcttt gctggcgaat gcttcgtcag gaacctgatc cagtgccacg acatcaccgg
    14341 taatcggcga taccagctcc gcgatagata ccgcgtttgg tacagcctgc ggttttgcta
    14401 caggcgcggc agttgccgga gttgcttcag ctgacgcagc ggctaccgga ccacgggcaa
    14461 cgactttctt catcgcatcg ccgatggatt ctgctttcgc gccaacaatc acctgaatag
    14521 tctgtttgtt cagtttcact acaccagaag cacccagacg tttacacatc gtatcgttga
    14581 cgcgggcaga gtcagccacg gtcaggcgca gacgggtaat acaggcatca atcgctttca
    14641 ggttgtcagt accgccaacc gcagcaatat agttggttgc cagttgagtc agaccttctt
    14701 cagtattgct gttggcttct tcagtaacga tctcgtcttc tttatcttca cgacccggcg
    14761 ttttcaggtt gaacatgcgg ataaccaaac tgaacaccac gaagtagata gcgaagaaga
    14821 taacgcccat caccagcagc atccagacgt tctggctggc ggcaggcagg ttatacatca
    14881 acgcgtagtc gatagcaccc gcagagaaag agaagccggc atggataccc agcagcgttg
    14941 ccacaaacag gctgataccg gtcagcagtg cgtgcaggag gtacagcagc ggcgcaagga
    15001 acatgaacag gaattccagc ggctcagtca caccggtcag gaacgcagta acagcaacag
    15061 aaagcagcat cccgccaacc atcggacgac gctctttcgg tgctgcgaag tacatcgcca
    15121 gcgccgcacc cggcagaccg aacatcatga tcgggaagaa gccggacatg aacatccccg
    15181 cggtgccgtc accagcgtag aaacggttga tgtcgccgtg gaaaaccgta cccgccgcgt
    15241 tggtgaattc accaatctgg aaccaggcga tggtgttcag cacctgatgc agaccggttg
    15301 ggatcagcag acggttgatg aaaccaaaga taccggaacc cagcgcgccc gcagaaacga
    15361 tccactcgcc gcctgcatgg atagcgtgct gtaccggcgg ccagacgtaa ccaaaaatgg
    15421 ccgccagcac cagacagaag aagccggtgg cgatcggcac aaagcgtttg ccgccgaaga
    15481 agctcaggaa gtccggcagt ttaatatcgg accaacggtt ataggctgcg ccaccaacca
    15541 gaccggtaat gatacccgcc agtacaccca tgttaatttc tgggttgatg gtcaccatcg
    15601 ctttggttaa cacaaagtaa cctaccgcac ccgccagcgc cgccgcacct gcgctgtctt
    15661 tcgaccagct ggatgccaca ccgatggcga agattaatgc gaggttatca aaaattgcac
    15721 cgcccgcctg ggcaataaac gcaacgttaa gtaaatctgg ctgaccgaat cgcagcaaca
    15781 gcgccgccac cggcagcacc gcgataggga gctgtaacgc cctaccgagt cgctggaaaa
    15841 aacctaaaat attcatctta ttccccctac gagaacccta tttggctcgt ttaaagccgt
    15901 atttttattt tgctgcaaat tttactgccg atgttctgta atcagattgt tagatcatct
    15961 gctacagagt gtgtgaaaat ttaattcgca tcgcaaatta aacgcgtgtc ttttgtgagt
    16021 tttgtcacca aatatcgtta tcatcactcc cttttactgg ctaaaccaga aaacttattt
    16081 tatcattcaa aaaatcaagt cggattgacg cctgtctgcg caaatccagg ttacgcttaa
    16141 agatgtctaa tccgccaacg gcttacattt tacttattga ggtgaataat gagactgatc
    16201 cccctgacta ccgctgaaca ggtcggcaaa tgggctgctc gccatatcgt caatcgtatc
    16261 aatgcgttca aaccaactgc cgatcgtccg tttgtactgg gcctgccgac tggcggcacg
    16321 ccgatgacca cctataaagc gttagtcgaa atgcataaag caggccaggt cagctttaag
    16381 cacgttgtca ccttcaacat ggacgaatat gtcggtctgc cgaaagagca tccggaaagc
    16441 tactacagct ttatgcaccg taatttcttc gatcacgttg atattccagc agaaaacatc
    16501 aaccttctca acggcaatgc cccggatatc gatgccgagt gccgccagta tgaagaaaaa
    16561 atccgttctt acggaaaaat tcatctgttt atgggcggtg taggtaacga cggtcatatt
    16621 gcatttaacg aaccggcgtc ttctctggct tctcgtactc gtatcaaaac cctgactcat
    16681 gacactcgcg tcgcgaactc tcgtttcttt gataacgatg tcaatcaggt gccaaaatat
    16741 gccctgactg tcggtgttgg tacactgctg gatgccgaag aagtgatgat tatggtgctg
    16801 ggtagccaga aagcactggc gctgcaggcc gccgttgaag gttgcgtgaa ccatatgtgg
    16861 accatcagct gtctgcaact gcatccgaaa gcgatcatgg tgtgcgatga accttccacc
    16921 atggagctga aagttaagac tttaagatat ttcaatgaat tagaagcaga aaatatcaaa
    16981 ggtctgtaat tgttatccct gccctggttc cttgctcagg gcaatatttt ttaaaatcgg
    17041 ggggcagaat gtatgcatta acccagggcc ggatctttac cggccacgaa tttcttgatg
    17101 accacgcggt tgttatcgct gatggcctga ttaaaagcgt ctgtccggta gcggaactgc
    17161 cgccagagat cgaacaacgt tcactgaacg gggccattct ctcccccggt tttatcgatg
    17221 tgcagttaaa cggctgcggc ggcgtacagt ttaacgacac cgctgaagcg gtcagcgtgg
    17281 aaacgctgga aatcatgcag aaagccaatg agaaatcagg ctgtactaac tatctgccga
    17341 cgcttatcac caccagcgat gagctgatga aacagggcgt gcgcgttatg cgtgagtacc
    17401 tggcaaaaca tccgaatcag gcgttaggtc tgcatctgga aggtccgtgg ctgaatctgg
    17461 taaaaaaagg cacccataat ccgaattttg tgcgtaagcc tgatgccgcg ctggtcgatt
    17521 tcctgtgtga aaacgccgac gtcattacca aagtgaccct ggcaccggaa atggttcctg
    17581 cggaagtcat cagcaaactg gcaaatgccg ggattgtggt ttctgccggt cactccaacg
    17641 cgacgttgaa agaagcaaaa gccggtttcc gcgcggggat tacctttgcc acccatctgt
    17701 acaacgcgat gccgtatatt accggtcgtg aaccgggcct ggcgggcgcg atcctcgacg
    17761 aagctgacat ttattgcggt attatcgctg atggcctgca tgttgattac gccaacattc
    17821 gtaacgctaa acgccttaaa ggcgacaaac tgtgtctggt taccgacgcc accgcgccag
    17881 caggtgccaa cattgaacag ttcatttttg cgggtaaaac aatatactac cgtaacggac
    17941 tttgtgtgga tgagaacggt acgttaagcg gttcatcctt aaccatgatt gaaggcgtgc
    18001 gtaatctggt cgaacattgc ggtatcgcac tggatgaagt gctgcgtatg gcgacgctct
    18061 atccggcgcg tgcgattgac gttgaaaaac gtctcggcac gctcgccgca ggtaaagtag
    18121 ccaacctgac cgcattcaca cctgatttta aaatcaccaa gaccatcgtt aacggtaacg
    18181 aggtcgtaac tcaataagag aaagtatgac accaggcgga caagctcaga taggtaatgt
    18241 tgatctcgta aaacagctta acagcgcggc ggtttatcgc ctgattgacc agtacgggcc
    18301 aatctcgcgg attcagattg ccgagcaaag ccagcttgcc cccgccagcg taaccaaaat
    18361 tacgcgtcag cttatcgaac gcgggctgat caaagaagtt gatcagcagg cctccaccgg
    18421 gggccgccgc gctatctcca tcgtcaccga aacccgcaat ttccacgcaa tcggcgtacg
    18481 gcttggtcgt catgacgcca ccatcactct gtttgatctc agcagcaaag tgctggcaga
    18541 agaacattac ccgctgccgg aacgtaccca gcagacgctg gaacatgccc tgctgaatgc
    18601 cattgctcag tttattgata gctaccagcg caagctgcgc gagctaatcg ccatttcggt
    18661 gatcctgcca gggcttgttg acccggacag cggcaaaatt cattacatgc cgcatattca
    18721 ggtagaaaac tgggggctgg tagaagcact ggaagaacgt tttaaagtga cctgtttcgt
    18781 tggtcacgat atccgtagtc tggcgctggc ggagcactac ttcggtgcaa gtcaggattg
    18841 cgaagactcc attctggtgc gtgtccatcg cggaaccggg gccgggatta tctctaacgg
    18901 gcgcattttt attggccgca acggtaacgt cggtgaaatt ggccatattc aggtcgaacc
    18961 gctgggtgaa cgctgccact gcggcaactt tggctgcctg gaaactatcg ccgccaacgc
    19021 tgccattgaa caacgggtgt tgaatctgtt aaagcagggc taccagagcc gtgtgccact
    19081 ggacgactgc accatcaaaa ctatctgcaa agccgcgaac aaaggcgata gcctggcctc
    19141 ggaagtgatt gagtatgtcg gtcgtcatct gggtaaaacc atcgccattg ctatcaactt
    19201 atttaatccg caaaaaattg ttattgctgg tgaaatcacc gaagccgata aagtgctgct
    19261 ccctgctatt gaaagctgca ttaataccca agcgctgaag gcgtttcgta ctaatctgcc
    19321 ggtggtacgt tctgagctgg atcaccgctc ggcaatcggc gcttttgcgc tggtaaaacg
    19381 cgccatgctc aacggtattt tgctccagca tttgctggaa aattaatgtg cttttatagt
    19441 ggcgcttatt gttgtcaata ttctgggtag tccatgacca ttaaaaatgt aatttgcgat
    19501 atcgacggcg tgctgatgca cgataacgtc gccgtaccgg gtgcagcgga atttttgcac
    19561 gggattatgg ataaaggcct gccgctggtg ttgctgacca actatccttc gcagactggg
    19621 caagatctgg cgaaccgctt tgccactgcc ggtgtcgatg tgccggatag cgtgttttat
    19681 acctctgcga tggcgaccgc cgatttcctg cgtcgctagg aaggcaagaa agcgtatgtg
    19741 gtgggcgaag gcgcactgat tcatgaactg tacaaagccg gtttcactat taccgatgtg
    19801 aaccctgatt tcgtgattgt tggcgaaaca cgttcctaca actgggacat gattcataaa
    19861 gcagcctatt tcgtcgctaa cggtgcacgc tttatcgcca ccaacccgga cacccacggg
    19921 cgcggttttt atcccgcttg tggcgcgttg tgtgcaggga ttgagaaaat ctccgggcgc
    19981 aaaccgttct atgttggtaa gcccagcccg tggatcatcc gcgcggcatt aaacaaaatg
    20041 caggcgcatt cggaagaaac agtgattgtc ggcgataacc tgcgtaccga tattctggcg
    20101 ggcttccagg ccggtctgga gacgattctg gtgctttctg gtgtttcatc gctcgacgat
    20161 atcgacagta tgccgttccg cccaagctgg atttacccgt cagtcgctga aatcgacgtt
    20221 atctgaatac cacccacgtc tgcggacgtg gttggctctg atgacaaacg ccaaactgcc
    20281 tgatgcgcta cgcttatcag gcctacgcag ctcctgcaat atattgaatt ttcatgcttt
    20341 tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa caaatcgcac tttatcagca
    20401 atttggcccc cgtaaaatat aaccattcaa taaaacccgc taaaaattat cgattcatca
    20461 ataaatacat cctcacacca gacccttttt caccaatcga caatcaccat tacgtttttt
    20521 atttttgcac tgccaaatag cttgcgcaca gtgctgcttt gcggcatttt tttaaacaag
    20581 caaacacaac aagcaacaaa taccaggtta acggagaagg ttatgtgttc aatttttggc
    20641 gtattcgata tcaaaacaga cgcagttgag ctgcgtaaga aagcactcga gctgtcacgc
    20701 ctgatgcgtc atcgtggccc ggactggtcc ggtatttatg ccagcgataa cgccattctc
    20761 gctcacgaac gtctgtcaat tgttgacgtt aacgcggggg cgcaacctct ctacaaccaa
    20821 caaaaaaacc acgtactggc ggtaaacggt gaaatctaca accaccaggc attgcgcgct
    20881 gaatatggcg atcgttatca gttccagaca gggtctgact gtgaagtgat cctcgcgctg
    20941 tatcaggaaa aagggccgga atttctcgac gacttgcagg gcatgtttgc ctttgccctg
    21001 tacgacagcg aaaaagatgc ctacctgatt ggtcgcgacc atctggggat catcccactg
    21061 tatatggggt atgacgaaca cggtcagctg tatgtggcct cagaaatgaa atcgctggtg
    21121 ccagtttgcc gcacgattaa agagttcccg gcggggagct atttgtggag tcaggacggc
    21181 gaaatccgtt cttattatca tcgcgactgg ttcgactttg atgcagtgaa agataacgtg
    21241 accgacaaaa acgagctgcg tcaggcactg gaagattccg ttaaaagcca tctgatgtct
    21301 gatgtgcctt acggtgtgct actttctggt ggtctggatt cctcaattat ttccgctatc
    21361 accaagaaat acgcagcccg tcgcgtggaa gatcaggaac gctctgaagc ctggtggccg
    21421 cagttacact cctttgctgt aggtctgccg ggttcaccgg atcttaaagc agcccaggaa
    21481 gtggcaaacc atctgggcac ggtgcatcac gaaattcact tcactgtaca ggaaggtctg
    21541 gatgccatcc gcgacgtgat ttaccacatc gaaacttatg atgtgaccac tattcgcgct
    21601 tcaacaccga tgtatttaat gtcgcgtaag atcaaggcga tgggcattaa aatggtgctg
    21661 tccggtgaag gttctgatga agtgtttggc ggttatcttt acttccataa agcgcccaac
    21721 gttaaagaac tgcatgaaga gacggtgcgt aaactgctgg ccttgcatat gtatgactgc
    21781 gcgcgcgcca acaaagcgat gtcagcctgg ggcgtggaag cacgcgttcc gttcctcgac
    21841 aaaaaattcc ttgatgtggc gatgcgcatt aacccgcagg ataaaatgtg cggtaacggc
    21901 aaaatggaaa aacacatcct gcgtgaatgt tttgagtcat acctgcccgc aagcgtggcc
    21961 tggcggcaga aagagcagtt ctccgatggc gtcggttaca gttggatcga caccctgaaa
    22021 gaagtggcgg cgcagcaggt ttctgatcag caactggaaa ctgcccgctt ccgcttcccg
    22081 tacaacacgc cgacctcaaa agaagcgtat ctgtaccgcg agatctttga agaactgttc
    22141 ccacttccga gtgccgctga gtgcgtgccg ggcggtcctt ccgtcgcgtg ttcttcagct
    22201 aaagcgatcg aatgggatga agcgttcaaa aaaatggacg atccgtctgg tcgtgcggtt
    22261 ggcgttcacc agtcggcgta taagtaagaa atgcggtact gccccggaga aatccggggc
    22321 tgttttagtt attgcgctgc ttgttgattc tgatatgcag taaggaaatg ttccgggttt
    22381 gccacatcac tgtttgacaa ctcatcactg taggccagta tttcatagat taatttccca
    22441 ccactattgt tgaattccga atttcatcct gtacgacaaa agaatggctg tagggtgggg
    22501 aaaaacacaa agtaaacaat aattgacgaa tatagcgcca cgctgttcgc aacctaacca
    22561 aacagtcact ttcgagcaat tttccttgaa aaagaggttg acgctgcaag gctctatacg
    22621 cataatgcgc cccgcaacgc cgataaggta tcgcgaaaaa aagatggcta cgtagctcag
    22681 ttggttagag cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac
    22741 catctttttt gcgggagtgg cgaaattggt agacgcacca gatttaggtt ctggcgccgc
    22801 aaggtgtgcg agttcaagtc tcgcctcccg caccattcac cagaaagcgt tgtacggatg
    22861 gggtatcgcc aagcggtaag gcaccggttt ttgataccgg cattccctgg ttcgaatcca
    22921 ggtaccccag ccatcttctt cgagtaagcg gttcaccgcc cggttattgg ggtatcgcca
    22981 agcggtaagg caccggtttt tgataccggc attccctggt tcgaatccag gtaccccagc
    23041 catcgaagaa ataatctggc tacgtagctc agttggttag agcacatcac tcataatgat
    23101 ggggtcacag gttcgaatcc cgtcgtagcc accaaattct gaatgtgtcg aatacgttcg
    23161 gcaaattcaa aaaccaattt gttggggtat cgccaagcgg taaggcaccg gattctgatt
    23221 ccggcattcc gaggttcgaa tcctcgtacc ccagccaatt tattcaagac gcttacatcg
    23281 taagtgcacc cagttggggt atcgccaagc ggtaaggcac cggattctga ttccggcatt
    23341 ccgaggttcg aatcctcgta ccccagccac attaaaaaag ctcgcttcgg cgagcttttt
    23401 gcttttctgc gttcattcaa tgtcgaatgc gatgttgaca cgtcttatcc ttcaatgtcg
    23461 gatgcgacgc tgccgcgtct tatccgacct acggctggca cgcatccggc aatgttgtaa
    23521 ggctacaacc ctaacgcata tttcagcgcc tgacgtttca acacgccagc acgctctgcc
    23581 gccattaacc cgagattacg cacaaaacgc agcggcggca gattattact gaatccggca
    23641 taaaacagat ccataccact ttgcataatg aagttatccg ccatgcgccg catctggtaa
    23701 cgcttgagga caggataact ggcccacgct tcaccgtagc tgcgggcatt caccagaaca
    23761 tcaatcaggg catcgacatc acgataacca agattcaccc cctgccccgc cagcggatgg
    23821 atggtatgcg cggcatcgcc caccagcgca agccccggct gcacatactg caacgcatgt
    23881 cggcgcgtca gcggaaacgc accagcggca agcggtgtaa cgtaacccag acgcgacggg
    23941 aaatgcttcg cgatttccgc ctggagttgc gccatattca tattctgcaa ctggcgaata
    24001 cgcgtcggag agtcatacca caccagcgat gcccagttat caaacaacgg cagaaacgca
    24061 cgcggtccgt ccggggtaaa ttgctgccag gtgctgtcgc cgggatcgtt ctcacactgt
    24121 acgctaatca acatgcacga ctgcgcatac tgccaggcat gaacgccaat tcccgccatc
    24181 tgccgcacct gagaatttgc gccgtcggca ccaatcacca gtttcgcgcg gatcgtttca
    24241 ccgcccttca gttccagctc ctgaagatca ttatctcgat gtaatgcaat cagcgaggtt
    24301 ggcacacgta acgttacttt cggatgcgct tccagcgcct gccacaacgc ctgttgcagg
    24361 actgtgtttt ccaccatgta gccaagcaac ggcaacttta attcggctgc gtcaaatacc
    24421 acatgtgccg tttcccactc ccaggtttcc agtctgcggt aaggatggca acgcatagcc
    24481 tgtactgcat cccagacccc taacccttta agcaatgata ccgaagccgc gctgatcgcc
    24541 gagatccgca cgtccggttg gctgtcagcg acaaacggtg caggttctgc gtgctcgatc
    24601 accgttaccg aaaatccgtg ctgtgccagc cccagcgcca gtgcgccgcc gaccattcct
    24661 ccgccgacaa tggcaatttc cgttggttga tttgtcatgg tcaatcatcc tgttagcaat
    24721 atgctttaag tttacaggat ttttaccccc aagggctgat aacgctcata ctggtcacaa
    24781 cggcagcaaa gcattacact atgcgccctg cattcctggc tactatttcg caagagcaag
    24841 tcgatgacca aaaaactcca tattaaaacc tggggctgtc agatgaacga gtacgattca
    24901 tcgaagatgg ccgatctgct ggatgccacc cacggctatc aactgaccga cgtggcggaa
    24961 gaagcggatg tgctactgct gaacacctgc tcaatccgcg agaaggctca ggaaaaagtc
    25021 ttccatcagt tgggtcgctg gaaactgtta aaagagaaga atccagacct gattatcggc
    25081 gtcggtggct gcgtggcatc gcaagaaggc gagcacattc gccagcgcgc ccactatgtc
    25141 gatattattt ttgggccgca aacgctgcac cgtctgccgg agatgatcaa ctccgtgcgc
    25201 ggcgaccgca gcccggttgt agatatcagc ttcccggaaa tcgagaagtt tgaccgtctg
    25261 ccggaaccgc gcgccgaagg gccgaccgcg tttgtctcca tcatggaagg ctgcaataaa
    25321 tattgcacct actgcgtggt gccttacacc cgtggtgaag aggtaagccg tccgtctgac
    25381 gatattctgt ttgagattgc ccagcttgcg gctcagggcg tgcgtgaagt caacctgctc
    25441 ggtcagaatg tgaatgcctg gcgtggtgag aactacgacg gcaccaccgg atcgtttgcc
    25501 gatctgttgc gtctggttgc tgcgatcgac gggatcgatc gtattcgctt taccaccagc
    25561 catccgatcg aattcaccga cgatatcatc gaagtgtacc gcgacacgcc tgagctggtg
    25621 agcttcctgc atctgccggt acagagcggt tccgatcgca ttctgaacct gatgggacgt
    25681 acccatacgg cgctggagta caaagcgatc atccgtaaac tgcgtgcggc gcgtccggat
    25741 attcagatca gttctgactt cattgttggc ttccctggcg aaaccaccga agacttcgag
    25801 aaaacgatga agctgattgc cgacgtcaat ttcgacatga gctacagctt tatcttctct
    25861 gcacgtccag gaacgcctgc cgccgatatg gttgatgatg ttccggaaga agagaagaag
    25921 cagcgtctgt atattctgca agaacgtatc aatcagcaag cgatggcatg gagccgtcgg
    25981 atgctcggca ccacccagcg tattctggta gaagggactt cgcgtaagag catcatggag
    26041 ctttccgggc gtaccgaaaa taaccgcgtg gtcaacttcg agggcacacc ggatatgatc
    26101 ggtaaattcg tcgatgtaga aattaccgac gtctacccga actctctgcg cggtaaagtg
    26161 gtgcgaactg aagatgaaat gggtctgcgc atggcagaaa caccggagtc ggtgattgcc
    26221 cgcacccgca aagaaaacga ccttggcgtg ggttattatc agccgtaatt ctcaggcctg
    26281 ccgttccggt gggccttgtt ttttctctct ttatccccat ctttatcgca atgcttgcgc
    26341 ctttttcctg tggcgtgaat aatttcctta taggccggtg aatgttcagc ttccggcggc
    26401 atacagataa gaggaacggt ttgaacatag acactcgcga aatcaccctg gagccagcag
    26461 acaatgcgcg tctgttgagc ctgtgcggcc cgtttgatga caacatcaag cagctcgaac
    26521 gccgtctcgg catcgagatc aatcgccgcg ataaccactt taaactgacc ggccgtccga
    26581 tttgcgtcac cgctgcggca gacattctgc gtagcctgta tgtcgatact gccccgatgc
    26641 gcggtcagat tcaggatatc gaaccggaac agatccacct tgcgattaaa gaagcgcggg
    26701 tactggagca aagcgcggag agcgtgccgg agtacggcaa agcggtcaat atcaaaacta
    26761 aacgcggcgt aattaagccg cgcacgccaa accaggcgca gtacatcgcc aatattctcg
    26821 accatgacat taccttcggc gttggcccgg cgggtacggg taaaacctac ctggcagtgg
    26881 ctgcggcagt tgatgccctg gagcgtcagg aaattcgccg tattctgctg actcgtccgg
    26941 cggtcgaagc cggtgagaaa ctgggcttcc tgcctggcga tttaagccag aaagtagacc
    27001 cgtatctgcg cccactgtac gacgcgctgt ttgaaatgct gggctttgag aaagtcgaga
    27061 aactgattga gcgcaacgtt attgaagtcg cgccgctggc ctatatgcgt ggtcgtacgc
    27121 tgaacgacgc gtttatcatt ctcgatgaga gccagaacac taccatcgaa cagatgaaga
    27181 tgttcctgac ccgtatcggt tttaactcaa aagcggttat caccggcgac gtcacacaga
    27241 tcgacctgcc gcgtaatact aaatcaggct tacgtcatgc cattgaagtg ctggccgatg
    27301 tcgaagagat cagctttaac ttcttccaca gcgaagacgt ggttcgtcac ccggtggtgg
    27361 cgcgtatcgt taacgcttat gaagcctggg aagaagcaga acaaaaacgt aaagcggcgc
    27421 tggcagcaga acgcaagcgc gaagaacagg aacaaaaatg agtcaggtga tcctcgattt
    27481 acaactggca tgtgaagata attccgggtt accggaagag agccagtttc aaacatggct
    27541 gaatgcggtg atcccgcagt ttcaggaaga gtcggaagtg actattcgcg tggtcgatac
    27601 cgccgaaagc cacagtctga atctgaccta tcgcggtaag gataagccga ccaacgtgct
//
LOCUS       DAZSNM010000028        27601 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZSNM010000028 DAZSNM010000000
VERSION     DAZSNM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.028"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="ACHW42_001071"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="ACHW42_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR8942982.1"
                     /translation="MAESTVTADSKLTSSDTRHRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="ACHW42_001072"
     CDS             complement(1614..1937)
                     /locus_tag="ACHW42_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR8942983.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHW42_001073"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHW42_001073"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR8942984.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHW42_001074"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHW42_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR8942985.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHW42_001075"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHW42_001075"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR8942986.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHW42_001076"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHW42_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR8942987.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="ACHW42_001077"
     CDS             7537..8574
                     /locus_tag="ACHW42_001077"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR8942988.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHW42_001078"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHW42_001078"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR8942989.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="ACHW42_001079"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="ACHW42_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR8942990.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHW42_001080"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHW42_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR8942991.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="ACHW42_001081"
     CDS             10745..11626
                     /locus_tag="ACHW42_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8942992.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHW42_001082"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHW42_001082"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR8942993.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="ACHW42_001083"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="ACHW42_001083"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR8942994.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHW42_001084"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHW42_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="ACHW42_001085"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="ACHW42_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR8942995.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHW42_001086"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHW42_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR8942996.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="ACHW42_001087"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="ACHW42_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR8942997.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="ACHW42_001088"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="ACHW42_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR8942998.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="ACHW42_001089"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="ACHW42_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR8942999.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="ACHW42_001090"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="ACHW42_001090"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR8943000.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="ACHW42_001091"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="ACHW42_001091"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR8943001.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="ACHW42_001092"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="ACHW42_001092"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR8943002.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="ACHW42_001093"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="ACHW42_001093"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR8943003.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="ACHW42_001094"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="ACHW42_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR8943004.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHW42_001095"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHW42_001095"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR8943005.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /gene="acpS"
                     /locus_tag="ACHW42_001096"
     CDS             24893..25273
                     /gene="acpS"
                     /locus_tag="ACHW42_001096"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR8943006.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="ACHW42_001097"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="ACHW42_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR8943007.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACHW42_001098"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACHW42_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR8943008.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="ACHW42_001099"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="ACHW42_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6808 a         6865 c         7236 g         6692 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcaccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAZSNM010000029        27505 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZSNM010000029 DAZSNM010000000
VERSION     DAZSNM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27505
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.029"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..192
                     /gene="ybeY"
                     /locus_tag="ACHW42_001100"
     CDS             <1..192
                     /gene="ybeY"
                     /locus_tag="ACHW42_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR8943009.1"
                     /translation="VEKEAQEQGKPLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAL
                     ETEIMLALGYEDPYIAEKE"
     gene            282..1160
                     /gene="corC"
                     /locus_tag="ACHW42_001101"
     CDS             282..1160
                     /gene="corC"
                     /locus_tag="ACHW42_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR8943010.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            1185..2723
                     /gene="lnt"
                     /locus_tag="ACHW42_001102"
     CDS             1185..2723
                     /gene="lnt"
                     /locus_tag="ACHW42_001102"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR8943011.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            3120..4028
                     /gene="gltI"
                     /locus_tag="ACHW42_001103"
     CDS             3120..4028
                     /gene="gltI"
                     /locus_tag="ACHW42_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR8943012.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            4198..4938
                     /gene="gltJ"
                     /locus_tag="ACHW42_001104"
     CDS             4198..4938
                     /gene="gltJ"
                     /locus_tag="ACHW42_001104"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR8943013.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            4938..5612
                     /gene="gltK"
                     /locus_tag="ACHW42_001105"
     CDS             4938..5612
                     /gene="gltK"
                     /locus_tag="ACHW42_001105"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR8943014.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            5612..6337
                     /gene="gltL"
                     /locus_tag="ACHW42_001106"
     CDS             5612..6337
                     /gene="gltL"
                     /locus_tag="ACHW42_001106"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR8943015.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            6455..7390
                     /gene="rihA"
                     /locus_tag="ACHW42_001107"
     CDS             6455..7390
                     /gene="rihA"
                     /locus_tag="ACHW42_001107"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR8943016.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            7474..9144
                     /gene="hscC"
                     /locus_tag="ACHW42_001108"
                     /pseudo
     CDS             7474..9144
                     /gene="hscC"
                     /locus_tag="ACHW42_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(9204..10655)
                     /gene="djlC"
                     /locus_tag="ACHW42_001109"
     CDS             complement(9204..10655)
                     /gene="djlC"
                     /locus_tag="ACHW42_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR8943017.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(10652..11359)
                     /locus_tag="ACHW42_001110"
     CDS             complement(10652..11359)
                     /locus_tag="ACHW42_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR8943018.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            11461..12015
                     /locus_tag="ACHW42_001111"
     CDS             11461..12015
                     /locus_tag="ACHW42_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR8943019.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(12025..13452)
                     /locus_tag="ACHW42_001112"
     CDS             complement(12025..13452)
                     /locus_tag="ACHW42_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR8943020.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(13449..14156)
                     /locus_tag="ACHW42_001113"
     CDS             complement(13449..14156)
                     /locus_tag="ACHW42_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR8943021.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            14320..15297
                     /gene="ybeQ"
                     /locus_tag="ACHW42_001114"
     CDS             14320..15297
                     /gene="ybeQ"
                     /locus_tag="ACHW42_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR8943022.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(15367..15849)
                     /locus_tag="ACHW42_001115"
     CDS             complement(15367..15849)
                     /locus_tag="ACHW42_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR8943023.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            16084..18666
                     /gene="leuS"
                     /locus_tag="ACHW42_001116"
     CDS             16084..18666
                     /gene="leuS"
                     /locus_tag="ACHW42_001116"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR8943024.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            18681..19262
                     /gene="lptE"
                     /locus_tag="ACHW42_001117"
     CDS             18681..19262
                     /gene="lptE"
                     /locus_tag="ACHW42_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR8943025.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            19262..20293
                     /gene="holA"
                     /locus_tag="ACHW42_001118"
     CDS             19262..20293
                     /gene="holA"
                     /locus_tag="ACHW42_001118"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR8943026.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            20295..20936
                     /gene="nadD"
                     /locus_tag="ACHW42_001119"
     CDS             20295..20936
                     /gene="nadD"
                     /locus_tag="ACHW42_001119"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR8943027.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            20960..21571
                     /locus_tag="ACHW42_001120"
     CDS             20960..21571
                     /locus_tag="ACHW42_001120"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFR8943028.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            21831..22148
                     /gene="rsfS"
                     /locus_tag="ACHW42_001121"
     CDS             21831..22148
                     /gene="rsfS"
                     /locus_tag="ACHW42_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR8943029.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            22152..22619
                     /gene="rlmH"
                     /locus_tag="ACHW42_001122"
     CDS             22152..22619
                     /gene="rlmH"
                     /locus_tag="ACHW42_001122"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR8943030.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            22650..24551
                     /gene="mrdA"
                     /locus_tag="ACHW42_001123"
     CDS             22650..24551
                     /gene="mrdA"
                     /locus_tag="ACHW42_001123"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR8943031.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            24554..25666
                     /gene="mrdB"
                     /locus_tag="ACHW42_001124"
                     /gene_synonym="rodA"
     CDS             24554..25666
                     /gene="mrdB"
                     /locus_tag="ACHW42_001124"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR8943032.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            25677..26765
                     /gene="rlpA"
                     /locus_tag="ACHW42_001125"
     CDS             25677..26765
                     /gene="rlpA"
                     /locus_tag="ACHW42_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR8943033.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            26904..>27505
                     /locus_tag="ACHW42_001126"
     CDS             26904..>27505
                     /locus_tag="ACHW42_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFR8943034.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQA"
BASE COUNT         6851 a         6942 c         7013 g         6699 t
ORIGIN      
        1 gttgagaagg aagctcagga gcaaggcaaa ccactggagg cgcactgggc gcatatggtg
       61 gtgcacggca gtctgcattt gttaggttac gatcacattg aagatgacga agcagaagaa
      121 atggaagccc tcgaaacaga gattatgctt gctctgggct atgaggatcc gtacattgcc
      181 gagaaagaat aatttgccag gcatttgact ggcagacgtc cccgccgttg gtgattaacg
      241 acggcgtttt gattaactaa catgagaacc catacgacgc catgagcgac gacaattcac
      301 acagtagtga cacgataagc aacaagaagg gatttttctc cctgttactc agccaacttt
      361 tccacggtga accgaaaaac cgtgacgaac tgctggcgct gatccgtgat tccgggcaga
      421 acgaccttat cgacgaagat acgcgcgata tgctcgaagg ggtgatggac atcgcagacc
      481 aacgcgttcg cgacatcatg atcccccgct cccagatgat taccctgaaa cgcaaccaga
      541 cgctggacga atgccttgat gtcatcatcg agtccgccca ctcacgtttc ccggtgatta
      601 gcgaagacaa agatcacatt gaagggattc tgatggcgaa agacttgctg ccgtttatgc
      661 gcagcgatgc tgaagccttc agcatggaca aagtgttacg tcaggcggtt gtcgttcctg
      721 aaagtaagcg cgtagaccgg atgctgaaag agtttcgctc tcagcgttac cacatggcga
      781 tcgttattga cgaattcggt ggggtttccg gtctggtgac cattgaagac atcctggaac
      841 tgattgttgg tgagattgaa gacgagtatg acgaagaaga tgatatcgac ttccgtcagc
      901 tgagtcgtca tacctggacc gtgcgcgcac tggcttccat tgaagacttc aacgaagcgt
      961 tcggcaccca ctttagcgat gaagaagtcg acactatcgg tggtctggtg atgcaggcgt
     1021 ttgggcatct tccggcgcgt ggcgaaacta tcgacatcga cggttaccag ttcaaagtgg
     1081 cgatggccga tagtcggcgt attattcagg ttcatgtcaa aatcccggat gactcacccc
     1141 agccaaagct ggatgaataa aaccgaaact ggatagataa ctacatggat tttgcctcat
     1201 taattgaacg ccagcgcatt cgcctgctgc tggcgttatt attcggtgcc tgcggaacgc
     1261 tggccttctc tccttacgac gtctggcctg cggcgattat ttcgctgatg gggcttcagg
     1321 cgttgacctt taaccgtcgc ccactccagt ctgccgctat tggcttttgc tggggatttg
     1381 gcctctttgg cagcggtatt aactgggtct atgtcagcat cgcgaccttt ggcgggatgc
     1441 ctggcccggt taacatcttc ctggtggtgc tgctggcggc gtatttgtcg ctgtataccg
     1501 gactgtttgc tggcgtgctg tcgcgtctgt ggccgaaaac cacctggctg cgcgtggcga
     1561 ttgccacccc tgctctctgg caagtgaccg agtttctgcg cggttgggtg ctgactggct
     1621 tcccgtggtt acagttcggc tatagccaga ttgatggccc gttaaaaggg ctggcaccgc
     1681 taatgggcgt ggaagccatt aacttcctgc tgatgatggt tagtggcctg ctggcactgg
     1741 cgttggtcaa acgcaactgg cgtccgctgg tggtggccgt cgtgctgttt gccctaccct
     1801 tcccgctgcg ttacatccaa tggtttaccc cgcaaccgga gaaaaccatt caggtttcga
     1861 tggttcaggg cgatattccg caatcgctga aatgggacga aggccagctt cttaatacgc
     1921 taaagattta ctacaacgca acggcaccgc tgatgggcaa atcatcgttg attatctggc
     1981 cggagtcggc gataaccgat ctggaaatta atcagcaacc gttcctcaaa gcactggacg
     2041 gtgagttgcg tgataaaggt agctcgctgg taaccgggat tgtcgacgcg cgtctcaata
     2101 agcagaaccg ctacgatacc tacaacacca tcatcacgct gggtaaaggt gcaccgtaca
     2161 gctacgaatc agccgatcgc tataacaaaa accatctggt gccgtttggc gagtttgtcc
     2221 cgctggagtc gattctgcgt ccgttagcac cgttctttga tctgccgatg tcgtcgttca
     2281 gccgtgggcc atatatccag ccgccgctgt cggcaaatgg tattgagctt actgcggcta
     2341 tttgctacga gattattctc gccgagcaag tgcgcgataa cttccgcccg gataccgact
     2401 acctgctgac tatctccaac gatgcgtggt ttggtaaatc tattggtcca tggcaacact
     2461 tccagatggc gcgaatgcgt gcgctggagc tggcgcgccc actgttgcgc agcaccaaca
     2521 acggcattac ggcggtgatt ggcccgcagg gtgagattca ggcgatgatc ccgcagttcg
     2581 cccgcgaggt gttaaccact aacgtgacgc cgaccaccgg actcacacca tacgcacgta
     2641 ccggcaactg gccgctgtgg gtgctgacgg cactgtttgg ttttgctgct gtgttgatga
     2701 gtctgcgtca gcgacgtaaa taatcccttc ttatcgtgcc ggaatacatc ccatatttcg
     2761 gcacgctcac cggatgcggc atcagtgcat tatccggata acaatattct cctatccaac
     2821 tttggcacat ctattgcttt gttatacaag gcaaagcttg aaccagcatc agtacaaccc
     2881 ggttgcacca cagcaatcca ccggtagcac caaaagagtg cattttaaga attttgcttc
     2941 agaatggtgc ggtgcacctc gcaaaaacaa acaataaaac atcaatatct ttacatttag
     3001 ccaaactaaa tgttacctaa caggcacaac actgcacaat aaagttgcag acgataacaa
     3061 cacaaacact cacaacgggt atccatgcgt tcttaacgca gaagataaag gtgttggata
     3121 tgcaattacg taaacctgcc acagcaatcc tcgccctggc gctttccgca ggactggcac
     3181 aggcagatga cgccgccccg gcagcaggca gtacgctgga caaaatcgcc aaaaacggtg
     3241 tgattgtcgt cggtcaccgt gaatcttcag tacctttctc ttattacgac aatcagcaaa
     3301 aagtggtggg ttactcgcag gattactcca acgccattgt tgaagcagtg aaaaagaaac
     3361 tcaacaaacc ggacttgcag gtaaaactga ttccgattac ctcacaaaat cgtattccac
     3421 tgctgcaaaa cggcactttc gattttgaat gtggttctac caccaacaac gtcgaacgcc
     3481 aaaaacaggc ggctttctct gacactattt tcgtggtcgg tacgcgcctg ttgaccaaaa
     3541 agggcggcga tatcaaagat tttgccgacc tgaaaggcaa agccgtagtc gtcacttccg
     3601 gcactacctc tgaagttttg ctcaacaaac tgaatgaaga gcaaaaaatg aatatgcgca
     3661 tcatcagcgc caaagatcac ggtgactctt tccgcaccct ggaaagcggt cgtgccgttg
     3721 cctttatgat ggatgacgct ctgctggccg gtgaacgtgc gaaagcgaag aaaccagaca
     3781 actgggatat cgtcggcaag ccgcagtctc aggaagccta cggttgcatg ttgcgtaaag
     3841 acgatccgca gttcaaaaag ctgatggatg acactatcgc tcaggtgcag acctccggtg
     3901 aagcggaaaa atggtttgat aaatggttca aaaatccaat tccgccgaaa aacctgaaca
     3961 tgaatttcga actgtcagac gaaatgaaag cactgttcaa agaaccgaat gacaaggcac
     4021 tgaactaatt acaagaacca ggggcggaaa ttccagccct ctcgattgtt acgtagcacg
     4081 gacagactat acgcctgatg gtcgttcccc atcgggcctg aaaaccgcaa tacgctgggt
     4141 aacaatcttc gagggtagcg gttaacgctg ctaccctctt ttttctggag tagatttatg
     4201 tctatagact ggaactgggg tattttttta caacaagccc cgttcggcaa taccacctat
     4261 cttggttggg tctggagtgg ttttcaggtg acgatcgctt tgtcgatctg cgcctggatt
     4321 atcgctttcc tcgtcggttc atttttcggc attttacgta ccgttccaaa ccgttttctc
     4381 tccggtctgg gtacgttgta tgtcgaactg ttccgcaacg tgccgttgat tgtgcaattc
     4441 tttacctggt atctggtgat cccggagctg ctgccggaga aaatcggcat gtggtttaag
     4501 gccgagctgg atcccaatat tcagtttttc ctttcatcca tgctctgcct ggggctgttt
     4561 accgccgccc gtgtttgcga acaggtacgc gccgctattc agtcgctacc gcgtgggcag
     4621 aagaatgccg cgctggcgat gggcctgacg ctaccgcaga cttatcgata cgtactgctg
     4681 cctaatgctt atcgcgttat cgtcccgccg atgacttccg agatgatgaa cctggtgaaa
     4741 aactccgcca tcgcctcgac catcggtctg gtggatatgg cggcgcaggc gggtaaattg
     4801 ctcgactact cggctcacgc ctgggagtcg tttaccgcca tcacgcttgc gtatgtgttg
     4861 attaacgctt ttatcatgct ggtgatgacg ctggttgaac gtaaagttcg cctgcccggc
     4921 aacatggggg gcaaataatg tacgagtttg actggagttc cattgttcct tccctgccat
     4981 atctgctcga cgggctggtg atcaccctga aaatcaccgt cacggcagtc gtgattggta
     5041 ttttgtgggg taccatgctg gcggtgatgc gcttgtccag cttcgcgcct gtttcgtggt
     5101 ttgccaaagc ctacgttaac gtattccgct cgattccttt agtcatggtt ttgctgtggt
     5161 tttacttgat tgtgccggga tttctgcaaa atgtgctggg attatcgcca aaaaatgata
     5221 ttcgtttaat ttcggcgatg gtggcgtttt cgatgtttga agcggcctac tattcagaga
     5281 ttatccgtgc cggtattcaa agtatttccc gtggtcagtc gagcgccgcg ctggcgttgg
     5341 ggatgactca ctggcagtcg atgaaactga ttattctgcc gcaggctttc cgcgcgatgg
     5401 tgccgctgct gctcactcag ggcatcgtac tgttccagga tacctcgctg gtgtatgtgt
     5461 tatctctggc cgatttcttc cgtaccgcct caaccattgg tgagcgtgat ggtacgcagg
     5521 tcgagatgat cctgtttgcc ggatttgttt atttcgttat tagccttagc gcgtcgttgt
     5581 tggtcagcta cttgaaaaga aggacagcat aatgattacc ctgaaaaatg tttcaaaatg
     5641 gtatggtcac tttcaggtgc tgaccgactg ctcaaccgaa gtgaaaaaag gcgaagtggt
     5701 ggtggtttgc ggcccgtctg gttccggcaa atcaacgctg attaaaaccg tcaacggcct
     5761 cgaaccagtg cagcaaggtg aaatcaccgt cgatggtatc gtggttaacg acaagaaaac
     5821 cgatctggca aagctgcgct cccgcgtcgg gatggtattc cagcatttcg agctgttccc
     5881 tcatctgtcg attatcgaaa acctgaccct ggcgcaggtg aaagtgctta aacgcgataa
     5941 agcgcccgct cgtgaaaaag cattgaaatt gctggagcgc gtgggacttt ccgcacatgc
     6001 caataaattt ccggcgcagc tttccggcgg tcagcagcag cgcgtggcaa tcgctcgcgc
     6061 attgtgtatg gctcctattg cgatgctgtt cgacgaaccg acatcggcgc tggatccgga
     6121 gatgatcaac gaagtgctgg acgtgatggt ggaactggcg aacgaaggaa tgaccatgat
     6181 ggtggtgacc cacgaaatgg gctttgcccg caaagtggcg aatcgggtga tctttatgga
     6241 cgagggtaaa attgtcgaag actcgccgaa agacgctttc ttcgatgatc cgaaatcgga
     6301 ccgcgcaaaa gacttcctcg cgaaaatcct gcattaatca ctctggcgca catacccaat
     6361 tgcgcgccat ctcacgcttt atcgtttcgc ccctcgtatc tgttacatcc tggcgttacc
     6421 cttgtcgcaa agaagcacac aacaaggagc aacaatggca ctgccaattc tgttagattg
     6481 cgacccaggt catgatgacg ctatcgcaat agttctcgca ctcgcctcac cagagcttga
     6541 tgtcaaagca attacgtctt ccgccggaaa ccagacacca gaaaaaacct tacgcaatgt
     6601 tctgcgtatg ctgaccttgc ttaatcgcac cgatattccg gtggcaggcg gcgcggtaaa
     6661 accgttaatg cgtgagttga ttatcgcgga caatgtgcac ggcgaaagcg gtctcgacgg
     6721 cccggcatta ccggaaccga cgttcgcacc gcaaaactgt acggcggtgg agctgatggc
     6781 gaaaacgctg cgcgaaagtg cggaacctgt cacgattgtt tctaccggac cgcaaactaa
     6841 cgttgccttg ctgctcaata gccatccgga actgcatagc aaaattgccc gtatcgtgat
     6901 tatgggcggc gcaatggggc ttggtaactg gacgcctgcg gctgaattta atatttacgt
     6961 tgacccggaa gcggcagaaa ttgtcttcca gtcagggatc ccggtggtga tggccggtct
     7021 ggatgttact cataaagcgc aaatccacgt tgaagacacc gagcgtttcc gcgcgattgg
     7081 caaccctgtt tcaaccattg ttgccgaact gctggatttc ttcctcgaat atcataaaga
     7141 cgaaaaatgg ggctttgtcg gcgcaccgct gcatgaccca tgcaccatcg cctggctgtt
     7201 gaaacccgag ttgtttacca ctgttgagcg ctgggttggc gtggaaacac aggggaaata
     7261 tacccagggt atgacggttg ttgattatta ttatctgact ggcaataaac cgaatgccac
     7321 tgtaattgtc gatgttgatc gtcagggctt tgttgattta ctggctgatc gtctgaaatt
     7381 ttacgcttaa cactcgtcat tgattaagat caccacgcgt tacctgggtg gtcttaatct
     7441 atttatctct ttttctttca agatataaat gcaatggata atgcagaact cgccataggt
     7501 atcgatctcg gcactaccaa tagtttaatt gccgtctgga aagacggtgc cgcgcaatta
     7561 attccaaata agttcggtga atatttaaca ccatccataa ttagcatgga tgaaaataat
     7621 catattttag tcggaaaacc ggctgtatca cggcgtactt cgcatccgga taaaacggca
     7681 gcgttattta aacgtgcaat gggcagtaat accaactggc ggttaggcag cgacacattt
     7741 aacgcgccag aactgtcctc tttggtatta cgctcattaa aagaagatgc cgaagaattt
     7801 ctgcaacgtc cgattaaaga tgtggtgatc tccgttctgg cttatttcag cgatgaacaa
     7861 cgcaagcata cccgtttagc agcggactta gccgggttaa atgcggtacg cttaattaat
     7921 gaacccacag cagctgcgat ggcgtatggc ctgcataccc aacaaaatac ccgttcgctg
     7981 gtgtttgatc tcggtggcgg cacgtttgac gttacggtgc ttgagtacgc cacgccggtg
     8041 attgaagttc acgcctccgc tggcgacaac ttcctcggtg gcgaagattt tacccatatg
     8101 ttggtcgatg acgttttaaa gcgcgcggat gtcacaagga ccacgcttaa cgagagtgaa
     8161 ctggcagcct tgtacgcctg tgtggaagcg gcaaaatgta gcaatcaatc gccaatgcac
     8221 attcgctggt agtaccagga tgaaacgcgg gaatgcgaat tttacgagaa cgaactggaa
     8281 gatttgtggt tgccgctgct caatcgcttg cgagtgccga ttgaacaggc gttgcgcgat
     8341 gcgcgtctga agccgagtca aatcgacagt ctggtgctgg ttggcggcgc gtcacaaatg
     8401 ccgctggtgc agcgaatcgc cgtgcgtctg tttggcaaat taccgtatca aagttacgat
     8461 ccgagcacca ttgtcgcgct gggcgcagca atccaggccg cctgccgctt acgcagtgaa
     8521 gatattgaag aagtgatcct cactgatatt tgcccttact cgttgggcgt tgaggttaac
     8581 cgccagggcg tttccggcat tttctcgccg attattgaac gaaacaccac tgtgcccgtg
     8641 tcgcgtgtag aaacttattc aaccatgcac ccggaacagg attcaattac ggttaacgtc
     8701 tatcagggag aaaaccacaa agttaaaaac aacattctgg tggaatcctt cgatgtgccg
     8761 ttgaagaaaa ccggggctta tcagtcgatt gatattcgct ttagttatga tatcaacggg
     8821 ttgcttgaag ttgacgtgct tctggaagac ggcagcgtta agtccagagt gattaaccac
     8881 agcccggtaa cattgagcgc gcagcagatt gaagagagtc ggacgcggtt atccgcattg
     8941 aaaatttatc cgcgcgatat gctcatcaat cgcaccttta aagccaaact ggaagagttg
     9001 tgggcgcggg cgctgggtga cgagcgagaa gagattggcc gggtgatcac cgattttgat
     9061 gcggcgctgc agtcaaacga tatggcccgc gtcgatgagg tcaggcggcg ggcgagcgtt
     9121 tatttagcca ttgagacctc ataatcacgg ctgccggatg cggcgtaaat gctgcatccg
     9181 gcatcggcgc aaaaaacaca tcgtcaacta aacaacatcg cgattttgcc gagagcataa
     9241 cctataacca gcaccgttaa actgattttg ataatgctgg taatgctctg tggtttttca
     9301 ccctcatcat ttttttcttc atggttttca ggtgttggct catcgacatc ttcatcctcc
     9361 ggctcgagtt ttactttcgt caacagcatc cgggtgataa tggcctctaa tggatgagca
     9421 tctccgcctt ccagccttgt attaagacgg gccagcatct cttgtttcgg aaacgcttca
     9481 agtactacct gttccgctac ccgatacaaa tccagtaacg gtgaatcttc ttctgtctcg
     9541 ctaccgactc tcgatttcca ccacgcccat gctttgcgtt tgaagaataa cgactcaata
     9601 aacgattgac ctaacggcga taattttccg ctatgtaaaa tttccgccca taacagccgc
     9661 gtccgggcgc tctcaccagg aacatatttc agcggttgac cttgcgcatc taccatgctc
     9721 cgcgcttcga taaccatcac taataacgcg aaataatccg ggcaatgacg cttgctccac
     9781 tgcaacaaca aattatcagc ttgtgtagat gggtaacttt cccagtacgc gccgagatca
     9841 gcaagcaggc tttccccctc gccgcagtac aaacgttgtg cacactggta ataccgggca
     9901 tcttcgcttt ctggttcttc cgcttgccac tgtttggcta caggaatgag ttccaccata
     9961 cctgtttgca ccgagctgta ccagcgcaac agtttgcgat gtaactttgc atcatcagga
    10021 atgaaccacg gtccttccat cgccaaaaac gcagtgacat agtcaggatg gtaacggcag
    10081 caacgttcca gcgcgaagta atattcaaca gtctggttct gtaacgcgac gggatattcc
    10141 agaaggctaa ggaaatcgaa acaatcacca gcctgaatgg cctcaagaaa agcctccctt
    10201 tcttcctcat ccattccttc gctgtcatta aacgaatgcc agttcaaacg ctctgccaac
    10261 aaagaagcac aatttaatga cagatatcgc gcttctatag cgattgcaca cagcggccag
    10321 cgtaattgat cgacatcctc catgttccag gtatttaatt gctgaataaa tttctgccag
    10381 gcggagggct gaaaacgatc actttctgaa tccagtaatg tcctgaatgc acgtagtatt
    10441 tcatgttcag cggtggcatc tttttcttca tcatctgctt cctcgacagg atttaccgcc
    10501 agtcgcagcg cctcttcata ggcctggcgt aatgctttaa acccctgcgg atcggtttcg
    10561 ggatgacaca acggtaagcg agcaagataa gcctggcgga taatgtctat ttgcgtcgtg
    10621 ctttcaattt cgaggatttg ccaacatgtt ttcataggtc gctcacatcc ttaagcgtta
    10681 ccgggcattc aggctggaca gttaatggct gccagggaag cgtggcatag aaacggtcgc
    10741 ttcccgctgc cagctcttcg aacatccgac tcccggcgct atcgtatttg tacagggtta
    10801 gcgcctcgcg tagcgaagcg ttgtcttcgc actgagaaga ttgccagtag agacgcccta
    10861 gtgaataggc agaaaaatag tgcatccagc tgtgatagta atggtgagct cgtgcataga
    10921 cgcgcgattg cagccataaa ctctcttctt ccgtcagcca gttgttgagc acaccggccc
    10981 gtgacagaaa tcccattcga acataatccc aggctttgat tcccccttct ccacagcaca
    11041 tagcggtttg ggcaacgaat tgggtataaa tcaaccctct ctccgagcca ccagcaatta
    11101 atgctttcca ctcttcaggg gaagagcgtt gccatgtcag atataagcca gctaaatcat
    11161 tggcatgacc atcatcagtc atctgataaa tagtctgaat taaatcatca cgattattta
    11221 ttccccagga tgatgagaga tcgaattctt ttatcccggt atacattttt ggcgagtcat
    11281 gcgcgggttt gcaactctga ttaagtattt ccatgggcgc agaaagcgca aaaagaagat
    11341 attgttcttc cttattcatt ttttaataat cccttaagtg cattaactga ctactttttt
    11401 attattgatg agtataaact tgacgctttc aaaataaaaa gaaaatcgaa gcattcacac
    11461 atgaataaaa aattaatgta tatattcgca atttttatag ttgcagcaat tacctgtatt
    11521 agccaaccca agaaaacgat gttgcgtgat aaagccatgg tgaattatgc ctttgattat
    11581 ttaagatcac caggcagtct accgttaacc acggcagcca cggagctttc cgcgattcat
    11641 ggtcactcaa catcgcaata tcgccttggc gaattttatc ttcatggtag cgacggtaaa
    11701 ccactggatt atacacaggc gagatactgg tatgagcaat cagcagaaca ggaaaatccg
    11761 cgcgcacaaa gcaaactggg gtggatctac ctgaaaggcc tgggagtcaa acccgatacc
    11821 cgtaaagcaa ttctctggta taaggaagca gctgaacaag ggtatgctca tgctcaatat
    11881 actttgggtt tgatctacag aaatggcact ggtattaatg ttaaccatta tgaatctcaa
    11941 aaatggttaa aactggccgc caaacaacat tacaaaaatg cggaaagatt acttgccgga
    12001 cttcccgcac attaacgtcg actgtcaccc gaagagatgg agtattttcc ctatcaaacc
    12061 ggcaaagata aaaatgtaga aaataatttt gatgacgccc agacagccca gcccgccatc
    12121 atcaacgggc attggctgcg gttgcggctc cggctcgacg gtttcctgac cggtaaaatt
    12181 cgccgcctga attaataatc gacgaataat taatgcttcc agtggacaac gatcttctgc
    12241 atcaaggcgg ctcacaatcg gcattaatga ttcttctgtc ggttggctgg cctgcactaa
    12301 ttgtttggca actaaatata atggccataa gggatgttct gtatcacgcg tgagtgagat
    12361 ccgcggctta cgtcctttgt gcaaaaccat ctcgaccagt gaacgcccca gcgcagagag
    12421 tttttcatcg ctgagtattt cgtataaatg aaaacgcgta cgggcgctgt cgccggggac
    12481 ataaagtagc ggttttccct gatcgttaac cagatcacgc gcttcaatca tcatcaccac
    12541 taatgggtaa taatagaccc gatgctgacg gcaccattgc aacattaaag catccgcctg
    12601 ggtggaggga tactcgcgcc agtagtaaca aagttctgcc agcaggcttt cgccttctcc
    12661 acaatagacg cgttgtgcgt attcataata acgcgcatct tcattatcag gttcagcttc
    12721 ccgccacgac cgggcgtaat ccaggagttc ggggattccc aggcggcttg cgctatacca
    12781 gcggagtaaa tttctgtgca gttttgcatc atcggggata atcactgcgc gatgttgcgc
    12841 taaatacaat gtcagccatt ctggatgcga tgaccaaatc cgagcgaaca tttggtaaaa
    12901 atcgatggtg tcattttgca cggcaactgg cagatgcaga atggtctgga agttaaaaac
    12961 attccccttc gcaatggcat ataaaaagga ttccagttct tcttcgtcta tttccccgac
    13021 gttttcctcc tgcaaccacc gcaatctttc tgctaataac accacgcact cgaaggataa
    13081 atgggcagtg ttcatggcga ttgtgcacag cgaccagcgt aattcatcaa tctcttccat
    13141 cgagcaataa tttaattgct gaatgaatcg ctgccaggcg gagggcagaa aacgttcgct
    13201 atcagaggca agtaacgcgc gaaaggcgag cagaatttca tgttctgcta cctcatattc
    13261 ttctggttgc caaacagatt tagcaggcga ctgcgcaatc cgtagcgcct cctcatacgc
    13321 ctgacgaagt tgtttaaaac cctgcggatc ggtttctgga tgaaaggacg gtaacagcgc
    13381 cagataagcg cggcggataa tatcgacgtc agtcgtttcc tctatatcga ggatcttcca
    13441 gcaatttttc ataagtcgct catatcctta agcgtttccg ggcatgtggg gtagtcagcc
    13501 agtggttgcc aggacaaggt agggtaatac gcatcatcac tggcaaataa ttgataaaac
    13561 atatcattgc cggaatcgtc gtattcttta cgcgctaaga attcgcgtaa taaaggcaga
    13621 tgatcatctt cgggagactg ccaatattgc cgaccaaagg tataaccggc aaagtattgc
    13681 cgccagttac tataataacg tagagcccgt agatgaatac gcgactggat ccatagactt
    13741 tcttcttcac tcaaccagtt attaagtacg cccattcgac ttaaaaaacc catccggaca
    13801 taatcccatg ctttaattcc tccctcacca cagcattcgg ctgtgctggc gacaaattgc
    13861 gcataagcct gaccttgctc atttaattca gcaagatagt cacgccattc acatggagaa
    13921 taacgaaacc agcggtgata aaaccctgcc aaccgagcgg catgaccgtt ctcgatcatt
    13981 cggtgaatta tgcgcagcag gtcatcacgg tcatcaattc cccatgagga agaaaggtca
    14041 aaaaagcatg tacctaaata cattttgggg gatgaatgcg aaggtaaaca gcattcattg
    14101 cgtatttcca tcggagtgga aagtgcaaac aatatttttt gcgaccccat gtccatataa
    14161 tttatcctta aacattcatt taatgaatgt aaactacatt tcgaggctca gttatttata
    14221 cttttctgac gcaacataat acgcataaaa taaggcttac gcaaaaatgg aacacataaa
    14281 aatataatat attttatcta caaccataag gaaatgataa tgatttttac gtcaagttgc
    14341 tgcgataatt tatcaataga tgagattatc gaacgtgctg aaaaaggcga ttgcgaggct
    14401 caatatattg ttgggtttta ttataatcgc gatagcgcaa ttgactctcc ggacgacgaa
    14461 aaggcctttt actggctgaa gctggccgct gagcaaggtc attgtgaagc acagtattcc
    14521 ttagggcaaa agtataccga ggataaaagc cgtcataaag ataatgagca agccatcttc
    14581 tggctgaaaa aagctgccct acaaggccat actttcgctt ccaacgccct tggctggata
    14641 ctggatcgtg gagaagaccc caactataaa gaagcggtta cctggtatca gatagccgcg
    14701 gagagcggaa tgtcttatgc gcaaaataat cttggatgga tgtacagaaa tggcaacgga
    14761 gtcgcacaag actatacgct ggcatttttc tggtacaaac aagctgcatt acaaggccat
    14821 agttacgcac aagacaatct ggccgatctt tataaagacg gagaaggcgt tgctcaaaac
    14881 aagacactcg ccgcattctg gtatttgaaa agcgcacagc agggtaatcg gcacgcccag
    14941 tttcaaattg catgggatta taacgctggc gaaggggtgg accaggacta taagcaagcg
    15001 atgtactggt atctgaaggc tgccgctcag gaaagcgtcg gcgcttacgt caacatcggt
    15061 tatatgtata aacacggaca aggcgttgag aaagattatc aggctgcctt tgaatggttt
    15121 acgaaagccg ctgaatgcaa tgacgccact gcctggtata acctggccat tatgtatcac
    15181 tacggagaag gaagacctgt cgatctccga caggctctcg acctgtatcg taaagttcag
    15241 tcatccggaa ccagagatgt cagtcaggaa attcgtgaga ctgaagcttt actgtagaaa
    15301 taaaaacaag ggccggggaa tttcattctg cccatattct ctttattgct gtgcgaaatc
    15361 agactattac ggctcaaacg ggcgtctctg gaactggtca taaccacatt tcgggcatag
    15421 cgtcagcact tccggtgtgt agatcgggag atggaagtga catttctcgc agaccagatt
    15481 tcccagcccg accacttctc cgctgtgata aaccccatga tgattgaggt cctggaaaat
    15541 ttcgcgccat tcaagctgcg ttttatcggt gatgtctgcc agctcctgcc acaagctttc
    15601 tttaatcacc cgcataaaga cgctgtcaga ttcttctttc aggctctctt catagctcat
    15661 ggcgaactct tccaggtcac gtctgacagc tcgcgtcagc tcatcgatct cggttcgcgt
    15721 taactcccct gtttttatta cgcgctcgcg cgcctgttcc accagtgcgt cgatatcacg
    15781 ttcgccattg cgcaggcgtt cgttcagtga cgcgaccagt tcacggtaat attgagcaac
    15841 cttgttcatc gtttcatctc ccgggtcgtt aactgactat aataatagac gttctttgtt
    15901 ccttgctttg ctaatacgcc cacaaactct gtaaattaat gtacgaataa tccgggcatc
    15961 atttcaacgt cgagtgcgcg aaaggctgtt ttgacgcgga cgtttgggct atgctatgcg
    16021 gatctgaaaa accacctcaa cgctacattt gtagccgtat tgaaaacagg accactggct
    16081 gccatgcaag agcaataccg cccggaagag atagaatcca aagtacagct tcattgggat
    16141 gagaagcgca catttgaagt aaccgaagac gagagcaaag agaagtatta ctgcctgtct
    16201 atgcttccct atccttctgg tcgactacac atgggccacg tacgtaacta caccatcggt
    16261 gacgtgatcg cccgttacca gcgcatgctg ggcaaaaacg ttctgcagcc gatcggctgg
    16321 gacgcgtttg gtctgcctgc ggaaggcgcg gcggtgaaaa acaacaccgc tccggcaccg
    16381 tggacgtacg acaacatcgc gtatatgaaa aaccagctca aaatgctggg ctttggttat
    16441 gactggagcc gcgagctggc aacctgtacg ccggaatact accgttggga acagaaattc
    16501 ttcaccgagc tgtataaaaa aggcctggta tataaaaaga cttctgcggt caactggtgc
    16561 ccgaacgacc agaccgtact ggcgaacgaa caagttatcg acggctgctg ctggcgctgc
    16621 gataccaaag ttgaacgtaa agagatcccg cagtggttta tcaaaatcac tgcttacgct
    16681 gacgagctgc tcaacgatct ggataaactg gatcactggc cagacaccgt taaaaccatg
    16741 cagcgtaact ggatcggtcg ttccgaaggc gtggagatca ccttcaacgt taacgactat
    16801 gacaacacgc tgaccgttta cactacccgc ccggacacct ttatgggttg tacctacctg
    16861 gcggtagctg cgggtcatcc gctggcgcag aaagcggcgg aaaataatcc tgaactggcg
    16921 gcctttattg acgaatgccg taacaccaaa gttgccgaag ctgaaatggc gacgatggag
    16981 aaaaaaggcg tcgatactgg ctttaaagct gttcacccat taacgggcga agaaattccc
    17041 gtttgggcag caaacttcgt attgatggag tacggcacgg gcgcagttat ggcggttccg
    17101 ggtcacgacc agcgcgacta cgagtttgcc tctaaatacg gcctgaacat caaaccggtt
    17161 atcctggcgg ctgacggctc tgagccagat ctttctcagc aagccctgac tgaaaaaggc
    17221 gtgctgttca actctggcga gttcaacggt cttgaccatg aagcggcctt caacgccatc
    17281 gccgataaac tgactgcgat gggcgttggc gagcgtaaag tgaactaccg cctgcgcgac
    17341 tggggtgttt ctcgccagcg ttactggggc gcgccgattc cgatggtgac gctggaagac
    17401 ggtaccgtaa tgccaacccc ggacgaccag ctgccggtga tcctgccgga agatgtggtg
    17461 atggacggca ttaccagccc gattaaagca gatccggagt gggcaaaaac taccgttaac
    17521 ggtatgccag cgctgcgtga aaccgacact ttcgacacct ttatggagtc ctcctggtac
    17581 tatgcgcgct acacttgccc agagtacaaa gaaggtatgc tggattccga agcggctaac
    17641 tactggctgc cggtggatat ctacattggt ggtatcgaac acgccattat gcacctgctc
    17701 tacttccgct tcttccacaa actgatgcgt gatgcaggca tggtgaactc tgacgaacca
    17761 gcgaaacagt tgctgtgtca gggtatggtg ctggcagatg ccttctacta tgttggcgaa
    17821 aacggcgaac gtaactgggt ttccccggtt gatgctatcg ttgaacgtga cgagaaaggc
    17881 cgtatcgtga aagcgaaaga tgcggcaggc catgaactgg tttataccgg catgagcaaa
    17941 atgtccaagt cgaagaacaa cggtatcgac ccgcaggtga tggttgaacg ttacggcgcg
    18001 gacaccgttc gtctgtttat gatgtttgct tctccggctg atatgactct cgaatggcag
    18061 gaatccggtg tggaaggggc taaccgcttc ctgaaacgtg tctggaaact ggtgtacgag
    18121 cacacagcaa aaggtgatgt tgcggcactg aacgttgatg cgctgactga agatcagaaa
    18181 gcgctgcgtc gcgatgtgca taaaactatc gctaaagtga ccgatgatat cggccgtcgt
    18241 cagaccttca acaccgcaat tgcggcgatt atggagctga tgaacaaact ggcgaaagca
    18301 ccgaccgatg gcgagcagga tcgcgctctg atgcaggaag cactgcaggc cgttgtccgt
    18361 atgctcaacc cgttcacccc gcacatctgc ttcacgctgt ggcaggaact caaaggcgaa
    18421 ggcgatatcg acaacgcgcc gtggccggtt gctgacgaaa aagcgatggt ggaagactcc
    18481 acgctggtcg tggtgcaggt taacggtaaa gtccgtgcca aaatcaccgt cccggtggac
    18541 gcaacggaag aacaggttcg cgaacgtgct ggccaggaac atctggtagc aaaatatctt
    18601 gatggcgtta ctgtacgtaa agtgatttac gtaccaggta aactcctcaa tctggtcgtt
    18661 ggctaagcgc gggaggaagc gtgcgatatc tggcaacact gttgttatct ctggcggtgt
    18721 taatcaccgc cgggtgtggc tggcatctgc gtgacaccac gcaggttcct tccactatga
    18781 aggtcatgat cctggactca ggcgatccga acgggccatt aagccgtgcg gtgcgtaacc
    18841 agttacgtct gaatggtgtc gagttgcttg ataaagaaac cacgcgtaag gacgttccat
    18901 ccctgcgttt gggtaaagtg agcatcgcga aagataccgc atcggtattc cgtaacggtc
    18961 aaacagcaga gtatcagatg atcatgacgg tgaatgcgac cgtgttgatc cccggccgtg
    19021 atatctaccc gattagcgcc aaagtcttcc gttcattctt cgataacccg caaatggcgt
    19081 tagcgaaaga taacgaacaa gacatgatcg taaaagagat gtacgaccgt gctgccgaac
    19141 agctgattcg taagctgcca agcatccgtg ctgcggatat tcgttccgac gaagaacaga
    19201 cgtcgaccac aacggatact ccggcaacgc ctgcacgcgt ctccaccatg ctgggtaact
    19261 gatgattcgg ttgtacccgg aacaactccg cgcgcagctc aatgaagggc tgcgcgcggc
    19321 gtatctttta cttggtaacg atcctctgtt attgcaggaa agccaggacg ctgttcgcca
    19381 ggtagctgcg gcacaaggat tcgaagaaca ccacactttt tccattgatc ccaacactga
    19441 ctggaatgcg atcttttcgt tatgccaggc tatgagtctg tttgccagtc gacaaacgct
    19501 attgctgttg ttaccagaaa acggaccgaa tgcggcgatc aatgaacaac ttctcacact
    19561 caccggactt ctgcatgacg acctgctatt gatcgtccgc ggtaataaat taagcaaagc
    19621 gcaagaaaat gccgcctggt ttactgctct tgcgaatcgc agcgtgcagg tgacctgtca
    19681 gacaccggag caggctcagc ttccccgctg ggttgctgtg cgcgcaaaac agctcaactt
    19741 agaactggat gacgcggcga atcaggtgct ctgctactgt tatgaaggta acctgctggc
    19801 gctggctcag gcactggagc gtttatcgct gctctggcca gacggcaaac tgacattacc
    19861 gcgcgttgaa caggcggtga atgatgccgc gcatttcacc ccttttcatt gggttgatgc
    19921 tttgttgatg ggaaaaagta agcgcgcgtt gcatattctt cagcaactgc gtctggaagg
    19981 cagcgagccg gttattttgt tgcgcacatt acaacgtgaa ctgttgttac tggttaacct
    20041 gaaacgccag tctgcccata cgccactgcg tgcgttgttt gataagcatc gggtatggca
    20101 gaaccgccgg ggcatgatgg gcgaggcgtt aaatcgctta agtcagccgc agttacgtca
    20161 ggccgtgcaa ctcctgacac gaacggaact caccctcaaa caagattacg gtcagtcagt
    20221 gtgggcagag ctggaagggt tatctcttct gttgtgccat aaacccctgg cggacgtatt
    20281 tatcgacggt tgatatgaaa tctttacagg ctctgtttgg cggcaccttt gatccggtgc
    20341 actatggtca tctaaaaccc gtggaaacgc tggcgaattt gattggtctg acgcgggtca
    20401 caatcatccc taataatgtt cctccgcatc gtccccagcc ggaagcgaac agcgtgcagc
    20461 gtaaacacat gcttgaactg gcgattgccg acaagccatt atttactctt gatgaacgcg
    20521 agctaaagcg caatgccccc tcttacactg cgcaaacact gaaagagtgg cggcaggaac
    20581 aaggaccgga cgtgccgctg gcgtttatta ttggtcagga ttcattgctg acctttccga
    20641 cctggtacga atacgaaacg atactcgaca atgcacattt gatcgtctgt cggcgtccag
    20701 gttacccact tgaaatggcg caaccgcaat accagcaatg gctggaagat catttgacac
    20761 ataacccgga agatcttcac cttcagcctg ccggtaaaat ttatctggct gaaacgccgt
    20821 ggtttaacat ctcggcgacc atcatccgcg aacgtttgca aaacggtgaa tcgtgtgagg
    20881 atttattgcc ggaaccggta ttgacttaca ttaaccaaca aggcttgtat cgctgatacc
    20941 tgtcgttacg gagggcatga tgcgactgtg gttaattcgt catggcgaaa cacaagcgaa
    21001 tgtcgatggt ctgtacagcg gtcatgcgcc cacacccctg accgcacgcg gtattgagca
    21061 agcgcaaaat ctgcatacac tgctagatga tgtttccttt gacttggttt tatgcagtga
    21121 actggaacgg gcacagcata ccgcgcgact ggttctcagt gaccgccagc tccccgttca
    21181 tatcatccct gaactcaacg aaatgttttt tggcgactgg gagatgcgac atcatcgcga
    21241 cctcatgcaa gaagatgccg aaaactatag cgcgtggtgc aatgactggc agcatgctat
    21301 ccccacgaac ggtgaaggat ttcaggcatt ttcgcaacgt gtggaacgct ttatcgcaag
    21361 gcttagtgaa tatcagcact atcagaatat tttaatcgtc agccatcagg gcgtactgag
    21421 tctgttaatt gctcgtttaa ttggcatgcc tgccgagtct atgtggcatt ttcgcgttga
    21481 ccaggggtgt tggagcgcca ttgatatcaa ccaaaaattc gcgacgctac gcgtcctcaa
    21541 tagccgtgcc atcggggtcg agaacgcatg acttttctgt ttttttacgg gtaagccgca
    21601 acgaccattg acagccccgg gcaggctgat attctccgca gccagacttt ttccgccaga
    21661 cacgactttg tagaaattgt tttacaaaaa tggcgatgca atctgcggcg cggggtggga
    21721 tgatagccca ctttcgaaag ccgattcggc gacaattgtc ccgaaatcgc ctctggttca
    21781 ggtatactga cagaccattt ttatctattt gattcaccca gggggaaaac ttgcagggta
    21841 aagcactcca ggattttgtt atcgacaaaa ttgatgacct caaaggtcag gacatcatcg
    21901 ccttagacgt tcaaggcaaa tccagcatca ccgactgcat gatcatctgt acgggtacgt
    21961 ccagccgtca tgttatgtcc attgctgacc acgttgtgca ggagtctcgc gcagcgggcc
    22021 tgttaccgct cggcgtagaa ggtgaaaaca gcgccgactg gattgtcgtg gatttgggcg
    22081 atgtgattgt ccatgtcatg caggaagaga gccgtcgcct gtatgaactg gaaaaactct
    22141 ggagttaatg cgtgaagctg caacttgtcg ccgtgggaac gaaaatgccg gactgggtac
    22201 aaaccggttt taccgagtac ctgcgtcgtt ttccgaaaga tatgcccttc gagctgattg
    22261 aaattccggc cggcaaacgc ggcaagaatg cggacatcaa gcgcatactc gacaaagagg
    22321 gtgagcagat gttggcggcc gcaggcaaaa accgcattgt caccctcgat attccaggca
    22381 agccctggga tacgccgcag ttagccgctg agctggaacg ctggaagctg gatggtcgcg
    22441 acgtaagtct actgattggc gggcctgaag ggttgtcgcc tgcctgtaaa gcggcggctg
    22501 agcagagctg gtcgctgtcg gcgcttaccc tcccccatcc gctggttcgc gtgctggtcg
    22561 cagagagtct gtaccgggcg tggagcatca ccaccaacca tccttatcac cgtgagtgat
    22621 aagggagctt tgagtagaaa acgcagcgga tgaaactaca gaactctttt cgcgactata
    22681 cggcagagtc cgcgctgttt gtgcgccggg cgctggtcgc ctttttgggg attttgctgc
    22741 tgaccggcgt gcttatcgcc aacctgtata atctgcaaat tgttcgcttt accgactacc
    22801 agacccgctc taatgaaaac cgcattaagc tggtgcctat cgcgcccagc cgcggcatta
    22861 tctacgaccg taacggtatc cctctggccc tcaaccgcac tatctaccag atagaaatga
    22921 tgccggagaa agtcgataac gtgcagcaaa cgctggacgc tttgcgcagc gtggtagatc
    22981 tgaccgatga cgatattgct gcattccgaa aagagcgcgc acgttcacac cgtttcacct
    23041 ctattccggt gaaaactaac ctaaccgaag tacaagtagc tcgctttgcc gtcaatcagt
    23101 accgttttcc gggtgtcgaa gttaaaggct ataaacgtcg ttactatcct tacggttcgg
    23161 cgttgaccca cgtcatcggc tatgtgtcga aaatcaacga taaagacgtc gaacgcctga
    23221 ataatgacgg caaactggcc aactatgcgg caacgcatga tattggtaag ctgggcattg
    23281 agcgttacta tgaagacgtg ctgcacggtc agaccggtta tgaagaggtt gaagttaaca
    23341 accgtgggcg tgttatccgc cagttaaaag aagtaccacc gcaagccgga cacgatattt
    23401 acctgacgct ggatctcaaa ctccagcaat atattgaaac gctgctggcg ggtagccgcg
    23461 cagctgtggt tgttaccgaa ccgcgtacag gtggggtgct ggcgctggtt tccacgccta
    23521 gttatgaccc aaacctgttt gttgacggta tctccagcaa agattattcc gccttgctga
    23581 acgacccgaa tacaccgctg gtgaaccgcg ccacacaggg ggtttatcct cccgcgtcta
    23641 cagttaaacc ctatgtggcg gtttcggcat taagcgccgg ggtgatcacg cgcaatacga
    23701 cgctgtttga cccaggctgg tggcaactac cagggtcgga aaaacgttat cgtgactgga
    23761 aaaaatgggg ccacgggcgt ctgaatgtca caagatcgct ggaagaatct gcggatacct
    23821 tcttctatca ggtggcctac gatatgggga tcgatcgcct ctccgaatgg atgggtaaat
    23881 tcggttatgg tcattacacc ggtatcgacc tggcggaaga acgttccggc aacatgccta
    23941 cccgcgaatg gaaacagaaa cgctttaaaa aaccgtggta tcagggtgac accattccgg
    24001 ttggtatcgg ccagggttac tggacagcga ccccaatcca gatgagtaag gcactgatga
    24061 tcctgattaa tgacggtatc gtgaaggttc cgcatttgct gatgagcacc gccgaagacg
    24121 gcaaacaggt gccatgggta caaccgcatg aaccgcccgt cggcgatatt cattccggtt
    24181 actgggagct ggcgaaagac ggtatgtacg gtgttgctaa ccgccctaat ggtacggcga
    24241 ataaatactt tgccagcgca ccgtacaaaa ttgcggcgaa atctggtacc gctcaggtct
    24301 tcggtctgaa agcgaacgaa acctataatg cgcacaaaat tgccgagcgt ttacgtgacc
    24361 acaaactgat gaccgccttt gcgccataca acaatccgca agtggctgtc gccatgattc
    24421 tggagaacgg cggcgcgggt ccggcggttg gtacgctgat gcgccagatc ctcgaccaca
    24481 ttatgctggg tgataacaac accgatctgc ctgcggaaaa tccagcggtt gccgcagcgg
    24541 aggaccatta atcatgacgg ataatccgaa taaaaaaaca ttctgggata aagtccatct
    24601 cgatcccaca atgctgctga ttttactggc attgctggtt tacagcgccc tggttatctg
    24661 gagcgccagc ggtcaggata ttggcatgat ggagcgtaaa atcggccaaa tcgcgatggg
    24721 tctggtcatc atggtggtga tggcgcaaat tcctccacgc gtttatgaag gctgggcccc
    24781 ctatctctat atcatctgta ttattttgct ggtggcggta gatgctttcg gagccatctc
    24841 taaaggcgct caacgctggc tggacctcgg tattgttcgt tttcagccgt cggaaattgc
    24901 caaaatagcc gtaccactga tggttgcgcg ctttatcaac cgcgacgttt gcccgccatc
    24961 gttgaagaat actggcatcg cgctggtgct gatatttatg cccacgctgc tggtggctgc
    25021 acagcctgac ctgggaacat caatcctcgt tgcgctttcc ggtctgtttg tactgttcct
    25081 ctctggcctt agctggcgtc tgattggcgt cgcagtagtg ctggtagcgg cgttcattcc
    25141 gattctgtgg ttcttcctga tgcatgatta ccagcgccag cgcgtaatga tgctcctgga
    25201 cccggaatca gacccactcg gcgcgggcta tcacattatt cagtctaaaa ttgctattgg
    25261 ctccggcgga ttacgcggca aaggctggct gcacggcact cagtcacagc ttgaatttct
    25321 ccccgaacgc catactgact ttatcttcgc ggtactggcg gaagagctgg gattagtggg
    25381 cattctgatt ctgctcgctc tctacattct gctgatcatg cgcgggctgt ggatagccgc
    25441 cagagcgcaa accacctttg gtcgcgtcat ggctggtggc ttaatgctga tattattcgt
    25501 ttatgtcttc gtaaatattg gtatggtaag cggtattctg ccggttgtag gggttccgct
    25561 cccactggtc agttatggag gatcggcgct aattgtgctg atggctgggt tcgggattgt
    25621 aatgtcaatc cacacccaca ggaaaatgtt gtcgaaaagc gtgtaagagg tgcgcaatgc
    25681 gtaagcagtg gctcgggatc tgcatcgcgg caggaatgct cgcggcatgt acaagcgatg
    25741 atggtcagca acagacggta agtgtaccgc agcctgcggt atgtaacggc cccatagttg
    25801 aaattagcgg ggcggacccg cgtttcgaac cactgaacgc gacggcaaat caggattacc
    25861 agcgcgacgg taaaagctac aaaatcgtgc aggatccgtc tcgatttagc caggcgggac
    25921 tggcggcaat ctatgatgcc gaaccaggca gtaacctgac ggcctctggc gaagctttcg
    25981 atccgacaaa gctgacggcg gcccatccaa cgcttccgat ccccagctac gccagaatca
    26041 ctaacctggc taacgggcga atgatcgtgg tgcgcattaa tgatcgcggc ccttacggca
    26101 acgaccgcgt tatttcgctt tctcgcgcgg cagctgaccg tcttaacacg tcaaacaaca
    26161 ccaaagttcg tatcgatccg attattgtcg cccaggatgg ttcgctttct ggtcctggta
    26221 tggcttgtac cacagtcgcc aaacagactt acgccctgcc tgcacctccc gatttaagcg
    26281 gtggcgcggg aacaagttca gtgtctggcc cgcagggtga cattcttccg gtcagtaatt
    26341 cgacgctaaa aagcgaagat ccgaccggcg cgccggtaac cagtagcggt ttcctcggcg
    26401 ctccaacgac cttagcgcct ggtgtactgg aaggcagcga accgacgcct gcgccacagc
    26461 ccgttgttac agcttcgtcg acaacgcctg caacctcgcc tgcaatggtg acaccgcaag
    26521 ccgcctcgca aagcgccagc ggcaacttta tggtgcaagt cggggccgta agcgatcagg
    26581 ctcgtgcgca acagtaccaa cagcaactgg gacagaagtt cggcgtcccc ggtcgcgtaa
    26641 ctcaaaatgg cgcggtctgg cggatccagc ttggcccatt cgccagcaaa gccgaagcca
    26701 gtaccttgca gcaacgtttg caaaccgaag cccaattaca gtcatttatt actaccgcgc
    26761 agtagcgtaa agcaggcatc tgatgtgtca atttctgtaa atgacgttaa caaagtcacg
    26821 cggaaagtca gatgcctgcc ggtagtgcat ttgctatagt agggcacttt tttaattcca
    26881 tcacggatgt cgtagttcag accatgaata ccattttttc cgctcgtatc atgaagcgcc
    26941 tggcgctcac cacggctctt tgcacagcct ttatctctgc tgcacatgcc gatgacctga
    27001 atatcaaaac tatgatcccg ggtgtaccgc agatcgatgc ggagtcctac atcctgattg
    27061 actataactc cggcaaagtg ctcgccgaac agaacgcaga tgtccgccgc gatcctgcca
    27121 gcctgaccaa aatgatgacc agttacgtta tcggccaggc aatgaaagcc ggtaaattta
    27181 aagaaactga tttagtcact atcggcaacg acgcatgggc caccggtaac ccggtgttta
    27241 aaggttcttc gctgatgttc ctcaaaccgg gcatgcaggt tccggtttct cagctgatcc
    27301 gcggtattaa cctgcaatcg ggtaacgatg cttgtgtcgc catggccgat tttgccgctg
    27361 gtagccagga cgcttttgtt ggcttgatga acagctacgt taacgcactg ggcctgaaaa
    27421 atacccactt ccagacggta catggtctgg atgctgatgg tcagtacagc tccgcgcgag
    27481 atatggcgct gatcggccag gcatt
//
LOCUS       DAZSNM010000030        27068 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZSNM010000030 DAZSNM010000000
VERSION     DAZSNM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27068
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.030"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1322
                     /gene="metL"
                     /locus_tag="ACHW42_001127"
     CDS             <1..1322
                     /gene="metL"
                     /locus_tag="ACHW42_001127"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR8943035.1"
                     /translation="ALKILDEAGLPGELRLRQGLALVAMVGAGVTRNPLHCHRFWQQL
                     KGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNIGSRW
                     LELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEESL
                     FLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGFHVISANKLAGASDSNKYRQIHDA
                     FEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQFDGSVPF
                     TELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPAHCEG
                     GSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDYPLAS
                     LLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAIQSDINRLAQLL"
     gene            complement(1461..1595)
                     /locus_tag="ACHW42_001128"
     CDS             complement(1461..1595)
                     /locus_tag="ACHW42_001128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8943036.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            1671..2561
                     /gene="metF"
                     /locus_tag="ACHW42_001129"
     CDS             1671..2561
                     /gene="metF"
                     /locus_tag="ACHW42_001129"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR8943037.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            2890..5070
                     /gene="katG"
                     /locus_tag="ACHW42_001130"
     CDS             2890..5070
                     /gene="katG"
                     /locus_tag="ACHW42_001130"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR8943038.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            5164..6069
                     /gene="yijE"
                     /locus_tag="ACHW42_001131"
     CDS             5164..6069
                     /gene="yijE"
                     /locus_tag="ACHW42_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR8943039.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(6096..6703)
                     /locus_tag="ACHW42_001132"
                     /pseudo
     CDS             complement(6096..6703)
                     /locus_tag="ACHW42_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(6978..8079)
                     /gene="gldA"
                     /locus_tag="ACHW42_001133"
                     /pseudo
     CDS             complement(6978..8079)
                     /gene="gldA"
                     /locus_tag="ACHW42_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            complement(8090..8752)
                     /gene="fsa"
                     /locus_tag="ACHW42_001134"
                     /pseudo
     CDS             complement(8090..8752)
                     /gene="fsa"
                     /locus_tag="ACHW42_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(8764..11265)
                     /gene="ptsP"
                     /locus_tag="ACHW42_001135"
                     /pseudo
     CDS             complement(8764..11265)
                     /gene="ptsP"
                     /locus_tag="ACHW42_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            11574..12653
                     /locus_tag="ACHW42_001136"
     CDS             11574..12653
                     /locus_tag="ACHW42_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR8943040.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            12668..12988
                     /locus_tag="ACHW42_001137"
     CDS             12668..12988
                     /locus_tag="ACHW42_001137"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR8943041.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            13039..15336
                     /locus_tag="ACHW42_001138"
     CDS             13039..15336
                     /locus_tag="ACHW42_001138"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR8943042.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            15302..16180
                     /locus_tag="ACHW42_001139"
     CDS             15302..16180
                     /locus_tag="ACHW42_001139"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR8943043.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLTPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            16182..16523
                     /locus_tag="ACHW42_001140"
     CDS             16182..16523
                     /locus_tag="ACHW42_001140"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR8943044.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(16510..17361)
                     /locus_tag="ACHW42_001141"
     CDS             complement(16510..17361)
                     /locus_tag="ACHW42_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8943045.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(17510..19243)
                     /locus_tag="ACHW42_001142"
     CDS             complement(17510..19243)
                     /locus_tag="ACHW42_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR8943046.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(19426..22077)
                     /gene="ppc"
                     /locus_tag="ACHW42_001143"
     CDS             complement(19426..22077)
                     /gene="ppc"
                     /locus_tag="ACHW42_001143"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR8943047.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(22429..23580)
                     /gene="argE"
                     /locus_tag="ACHW42_001144"
     CDS             complement(22429..23580)
                     /gene="argE"
                     /locus_tag="ACHW42_001144"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR8943048.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            23734..24738
                     /gene="argC"
                     /locus_tag="ACHW42_001145"
     CDS             23734..24738
                     /gene="argC"
                     /locus_tag="ACHW42_001145"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR8943049.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            24746..25522
                     /gene="argB"
                     /locus_tag="ACHW42_001146"
     CDS             24746..25522
                     /gene="argB"
                     /locus_tag="ACHW42_001146"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR8943050.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            25583..26956
                     /gene="argH"
                     /locus_tag="ACHW42_001147"
     CDS             25583..26956
                     /gene="argH"
                     /locus_tag="ACHW42_001147"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR8943051.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         6118 a         6851 c         7577 g         6522 t
ORIGIN      
        1 gtgcgctgaa aatcctcgac gaagcgggat tacctggaga actgcgcctg cgtcaggggc
       61 tggcgctggt ggcgatggtc ggggcgggtg tcacccgtaa cccgctgcat tgccaccgct
      121 tctggcagca actgaaaggc cagccggtcg agtttacgtg gcagtccgat gacggcatca
      181 gtctggtggc agtactgcgc accggcccga ccgaaagcct gattcagggg ctgcatcagt
      241 ccgtcttccg cgcagaaaaa cgcatcggcc tggtattgtt cggcaaaggc aatatcggct
      301 ctcgctggct ggaactgttc gcccgcgagc agagcacgct ttcggcgcgt accggctttg
      361 aatttgtgct ggcaggcgtg gtggacagcc gccgcagtct gttgagttat gacgggctgg
      421 acgccagccg cgcgttagcc ttcttcaacg atgaagcggt cgagcaggat gaagagtcgc
      481 ttttcctgtg gatgcgcgcc catccgtatg atgatttagt ggtgttggac gttaccgcca
      541 gccagcagct tgctgatcag tatctcgatt tcgccagcca cggtttccac gtcatcagtg
      601 ccaacaaact ggcgggagca agcgacagca ataaatatcg ccagatccac gacgccttcg
      661 aaaaaaccgg gcgtcactgg ctatacaacg ccaccgttgg tgcgggcttg ccgatcaacc
      721 ataccgtgcg cgatctgatc gacagcggcg ataccatttt gtcgatcagt gggatcttct
      781 ccggcacgct ctcctggctg ttcctgcaat tcgacggtag cgtgccgttt accgagctgg
      841 tggatcaggc gtggcagcag ggcttaaccg agcctgatcc gcgtgacgac ctctccggca
      901 aagacgtaat gcgcaagctg gtgattctgg cgcgtgaagc gggttacaac attgaaccgg
      961 accaggtacg tgtggaatcg ctggtgcctg ctcattgcga aggcggcagc atcgaccatt
     1021 tctttgaaaa tggcgatgaa cttaacgagc agatggtgca acggctggaa gcggcccgcg
     1081 aaatggggct ggtgctgcgc tacgtggcgc gtttcgatgc caacggcaaa gcgcgtgtgg
     1141 gcgtggaggc ggtgcgtgaa gactatccgc tggcatcact gctgccatgc gataacgtct
     1201 ttgccatcga aagccgctgg taccgcgata atccgctggt gatccgtggg cctggcgctg
     1261 ggcgcgacgt caccgccggg gcgattcagt cggatatcaa ccggctggca cagttgttgt
     1321 aatttcagaa atttaataat gcccggtact catgttttcg ggtctatggt ttctaatgaa
     1381 atatattgaa ttatcataga attaggccgg atgaagcgtt tacgacgaat ccggcaagaa
     1441 gcaataagta catggttagt ttatatttgc agtccggttt gctttgcata tcggattttc
     1501 tttttcttac catcctgaag ttttttcatc ttccctgatt tttcctcacc atcattggtc
     1561 atttttcggt tgacgccctt cggcttttcc ttcatcttta catctggacg tctaaatgga
     1621 tagatgtgca caacacaaca tataactaca agcgattgat gaggtaaggt atgagctttt
     1681 ttcacgccag ccagcgggat gccctgaatc agagcctggc agaagtccag gggcagatta
     1741 acgtttcgtt cgagtttttc ccgccgcgta ccagtgaaat ggagcagacc ctgtggaact
     1801 ccatcgatcg ccttagcagc ctgaaaccga agtttgtatc ggtgacctat ggcgcgaact
     1861 ccggcgagcg cgaccgtacg cacagcatta ttaaaggcat taaagatcgc actggtctgg
     1921 aagcggcacc gcatcttact tgcattgatg cgacgcccga cgagctgcgc accattgcac
     1981 gcgactactg gaataacggt attcgtcata tcgtggcact gcgtggcgat ctgccgccgg
     2041 gaagtggtaa gccagaaatg tatgcttctg acctggtgac gctgttaaaa gaagtggcag
     2101 atttcgatat atccgtggcg gcgtatccgg aagttcaccc ggaagcaaaa agcgctcagg
     2161 cggatttgct taatctgaaa cgcaaagtgg atgccggagc caaccgcgcg attactcagt
     2221 tcttcttcga tgtcgaaagc tacctgcgtt ttcgtgaccg ctgtgtatcg gcgggcattg
     2281 atgtggaaat catcccgggc attttgccgg tatctaactt taaacaggcg aagaaatttg
     2341 ccgacatgac caacgtgcgt attccggcgt ggatggcgca aatgtttgac gggctggatg
     2401 acgatgccga aacccgcaaa ctggtgggcg cgaatattgc catggatatg gtgaagattt
     2461 taagccgtga aggagtgaaa gatttccact tctatacgct taaccgcgct gaaatgagtt
     2521 acgcgatttg ccatacgctg ggtgttcgac ctggtttata aatagtgtgg cttttgtgaa
     2581 aatcacacag tgatcacaaa ttttaaacag agcacaaaat gctgcctcga aatgagggcg
     2641 ggaaaataag gttgtcagcc ttgttttctc cctcattact tgaaggatat gaagctaaaa
     2701 ccctttttta taaagcattt gtccgaattc ggacataatc aaaaaagctt aattacgatc
     2761 aatttgatct acatctcttt aaccaacaat atgtaagatc tcaactatcg catccgtgga
     2821 ttaattcaat tataacttct ctctaacgct gtgtatcgta acggtaacac tgtagagggg
     2881 agcacattga tgagcacgtc agacgatatc cataacacca cagccactgg caaatgcccg
     2941 ttccatcagg gcggtcacga ccagagcgcg ggggcgggca caaccactcg cgactggtgg
     3001 ccaaatcaac tccgtgttga cctgttaaac caacattcta atcgttctaa cccgctgggt
     3061 gaggactttg actaccgcaa agaattcagc aaattagatt actacggcct gaaaaaagat
     3121 ctgaaagccc tgctgacaga atctcagccg tggtggccag ccgactgggg cagctacgcc
     3181 ggtctgttta ttcgtatggc ttggcacggc gcaggtactt accgttcaat cgatggacgc
     3241 ggcggcgcgg gtcgtggtca gcaacgtttt gcaccgctga actcctggcc ggataacgta
     3301 agcctcgata aagcgcgtcg cctgttgtgg ccaatcaaac agaaatatgg tcagaaaatc
     3361 tcctgggccg acctgtttat cctcgcaggt aacgtggcgc tggaaaactc cggcttccgt
     3421 accttcggtt ttggtgccgg tcgtgaagac gtctgggaac cggatctgga tgttaactgg
     3481 ggtgatgaaa aagcctggct gactcaccgt catccggaag cgctggcgaa agcaccgctg
     3541 ggtgcaaccg agatgggcct gatttacgtt aacccggaag gcccggatca cagcggcgaa
     3601 ccgctttctg cggcagcagc tatccgcgcg accttcggca acatgggcat gaacgacgaa
     3661 gaaaccgtgg cgctgattgc gggtggtcat acgctgggta aaacccacgg tgccggtccg
     3721 acatcaaacg taggtcctga tccagaagct gcaccgattg aagaacaagg tttaggctgg
     3781 gcgagcactt acggcagcgg cgttggcgca gatgccatta cctctggtct ggaagtagtc
     3841 tggacccaga cgccgatcca gtggagcaac tatttcttcg agaatctgtt caagtatgag
     3901 tgggtacaga cccgcagtcc ggctggagca atccagttcg aagcggtaga cgcaccggaa
     3961 attatcccgg atccgttcga tccgtcgaag aaacgtaaac cgacaatgct ggtgaccgac
     4021 ctgacgctgc gttttgatcc tgagttcgag aagatctctc gtcgtttcct caacgatccg
     4081 caggcgttca acgaagcctt tgcccgtgcc tggttcaaac tgacgcacag ggatatgggg
     4141 ccgaaatctc gctacatcgg gccggaagtg ccgaaagaag atctgatctg gcaagatccg
     4201 ctgccgcagc cgatctacaa cccgaccgag caggacatta tcgatctgaa attcgcgatt
     4261 gcggattctg gtctgtctgt tagtgagctg gtatcggtgg cctgggcatc tgcttctacc
     4321 ttccgtggtg gcgacaaacg cggtggtgcc aacggtgcgc gtctggcatt aatgccgcag
     4381 cgcgactggg atgtgaacgc cgcagccgtt cgtgctctgc ctgttctgga gaaaatccag
     4441 aaagagtctg gtaaagcctc gctggcggat atcatcgtgc tggctggtgt ggttggtgtt
     4501 gagaaagccg caagcgccgc aggtttgagc attcatgtac cgtttgcgcc gggtcgcgtt
     4561 gatgcgcgtc aggatcagac tgacattgag atgtttgagc tgctggagcc aattgctgac
     4621 ggtttccgta actatcgcgc tcgtctggac gtttccacca ccgagtcact gctgatcgac
     4681 aaagcacagc aactgacgct gaccgcgccg gaaatgactg cgctggtggg cgggatgcgt
     4741 gtactgggtg ccaacttcga tggcagcaaa aacggcgtct tcactgaccg cgttggcgta
     4801 ttgagcaatg acttcttcgt gaacttgctg gatatgcgtt acgagtggaa agcgactgac
     4861 gaatcgaaag agctgttcga aggacgtgac cgcgaaaccg gcgaagtgaa atacacagcc
     4921 agccgtgcgg atctggtatt tggttctaac tctgtcctgc gtgcagtagc ggaagtttac
     4981 gccagcagcg atgcccacga gaagtttgtt aaagacttcg tagcggcatg ggtgaaagtg
     5041 atgaacctcg accgtttcga cctgctgtaa tctgaccttc ttcagcgact gtctttcagg
     5101 cagtcgctga agtttcttta ccggcgtata gtgtccacag gaaaactaca cactggatct
     5161 ctcatgtctg ccgcaggaaa gagcaaccca ctggcaatca gtggcttggt tgtgctcaca
     5221 cttatctgga gttatagctg gattttcatg aagcaagtca ccagttacat cggtgccttc
     5281 gactttaccg ccttacgctg cattttcggc gctctcgttt tattcatcgt ccttttatta
     5341 cgtggtcgcg gaatgcgccc gacgccgttt aaatacacct tagccattgc cctgttacaa
     5401 acctgcggga tggttggtct ggcgcagtgg gcgttggtca gcggaggtgc ggggaaggtg
     5461 gcgatcctga gctataccat gccgttctgg gtggtgattt tcgccgcgtt gtttctcggt
     5521 gaacgcctgc gacgtgggca atatttcgcg attctgattg ccgctttcgg cttatttttg
     5581 gtgttgcagc cgtggcaact cgatttctct tcgatgaaaa gtgccatgct ggcaatcctc
     5641 tccggtgtta gttggggggc gagcgcgatt gttgctaaac gcctatacgc ccgtcatccg
     5701 cgcgtggatt tattatcgtt aacatcctgg cagatgctgt atgcggcgct ggtgatgagt
     5761 gtggtcgctt tactggtgcc gcaacgtgaa attgactggc agcccaccgt gttctgggcg
     5821 ctggcctaca gtgcgattct ggcgacggcg ctagcgtgga gcttatggct gtttgtgctg
     5881 aaaaacttgc ctgccagtat tgccagctta agcacactgg ccgttcccgt ttgtggcgta
     5941 ctcttttcct ggtggctgct cggtgagaat ccgggggccg ttgaaggtag cggtattgtg
     6001 ctgattgtgc tggcactggc gctggtgagc cgtaagaaaa aagaagctgt cagtgtaaaa
     6061 agcatctgaa ttttttcttc atgtggggcg atctcttatt taacaaaata acgataatgc
     6121 cccaccatcc gccagctaaa cagcacatct tcttcctgcg cgcctgcgcc aatgttatgt
     6181 atcaccagcg gcgtaccgtc gcgggcgaag ccatctgacg ccaccccaat atgcgccagc
     6241 ccgttatcta atcgccagga gacaatatcg cccgcttgat agtcaccggc atttttactg
     6301 atggggcgtg ttttgttgtg gcggctaaac caggtttcca gattaggcac ccgacggtga
     6361 tcgatgttgc tgtccgggcg ctttaacttc catttttgcg ggtactcagc aaaattcttc
     6421 gccatatctt cgtgaaccag tttctgcaaa tcgaccttct ggctgcgcaa tgcgcggatc
     6481 accatatcgg aacatacacc gcgttcttgc ggaacatcac cgccaggata ggtaagctgc
     6541 gatcgtaaaa tagcgtgctg ccaatttgct gtctggcacc gtctgcgatg gcaaggttgg
     6601 tattggcctg gatttgtacc acggttggtg gaacggcggg agattttaag gagtggctgg
     6661 taaatgccgt tagcaggctg agcagcgcca gtgaagcttt catctggttc gtccgtgaaa
     6721 ataagagtca cagattcgac cttccgggct aacgattgtt ctgtggagaa aatgtccaga
     6781 agcggataaa aaactcctga acaacgtggg agtttcagat tactggcaca gagtgccgga
     6841 tgcggagtga acgccttatc cggcctacaa aagcacgcaa attcaatata ttgcagagat
     6901 tgcgtaggcc tgataagcgt agcgcatcag gcaattttgc gttcaaactc ccggacaagc
     6961 cgggagtttg gagtaggtta ttcccactct tgcaggaaac gctgaccgta ctggtcagct
     7021 accagcagtg cggcgtaaac ctgatctggc gtcgcgccgc caggcatgtt gtggatggtt
     7081 tcaccttctg cacaagatgc ttttgcaaca atgcgcattt tcgccgggac atcttcttta
     7141 atatccagtt gagcgagagt tattggcaaa cctaccgcat ggctaagtgc agctacggtt
     7201 tcgatttcct ccaccggcgc gttttccagt accagctgcg tcagcgtacc aaatgccact
     7261 tttcaccgtg ataatagtga tgcgcgtccg ggatagcggt caggccgtta tgcaccgcgt
     7321 gagccgccgc cagccgccgc tttcaaaacc aacgccgctc aaataggtgt tcgcttcaat
     7381 cacgcgctcc agcgccgggg ttactacatg ctgttcggcg gcaagcatcg ctttttcgcc
     7441 ttcttccagc agggtgttgt agcacagttc agccagtgcc agcgccgcct gggtgcactt
     7501 gccgcccgcc atggtggtcg cgccgctacg agagcaagca cgcgcttcaa accaggttgc
     7561 cagcgcatcg ccgatacccg ctgctaacag acgtgcaggt gcgcccgcga cgattttggt
     7621 gtcgacaatg accatattcg ggttatttgg caacagcaga tagcggtcaa actcaccctc
     7681 atcggtgtag ataacagaca atgcgctgca cggtgcatcg gtcgaggcga tagtcggtgc
     7741 gatcgctacc ggaacaccca tgaaatgtgc cagtgctttg gcagtatcga gggtttttcc
     7801 gccaccgata ccgagaattg cgccacactg cgcagtctcc gcgatgccac gcagacggtc
     7861 gatctcattt tgcgaacatt caccgccaaa cggcgtaatt tctactacca gtccagcatc
     7921 tttaaagctt ttctcgacag tggattgagc aaaaactaaa acaaatttgt cacccaccac
     7981 taaccagcgt tctgccagcg gcttcaggta ttcgcccaga cgattaatca catcagcgcc
     8041 ctggatgtat ttacccggtg attgaataat gcggtccata attgctcctt tagagatgag
     8101 tagtgccaaa tgcggcattc cagtcgtgtt cgaacttctc tatagctgac tctaccgcag
     8161 gggtgttgag catttgttgc gctacatcta agggcagggt gatggattca catcctgcca
     8221 gtaaacagtc cagcgcctga cgcggcgttt taaagctggc tgccagcacc atgctttctg
     8281 gcgcgtgcat ttctaacagc gtttgcagct cctgaaccgt acgaatgccg tccccgccct
     8341 gggcatctac gcggttaaca tacggagcaa cgtattttgc ccctgccagt gcggctaata
     8401 acccttctgc ggcgctatat acagcggtgc caagtgtagt aataccctat tttttcagta
     8461 ttttaattgc tgccagacct tcggaagtca ccgggatttt caccacaata cccggaatag
     8521 cgtcgcgcag gcgcttcgct tcttccacca tcccctgcgc gtcgcggctc atggtctgag
     8581 caaacagaat gccctcatca ccaatcgctt tttgcagacg cggcagcact tcccagatgg
     8641 actccttgct ggcagcgata atgctcgggt tagttgtcac cccggcaatc gggaatatgc
     8701 gtgccagacg ttcgacttct gcgacgttag cggtgtccag atacagttcc atgatgtttc
     8761 cttttacagt tccagttcat gttgcagcag gctggcgata gcgtcggcag aggcggcgtt
     8821 aaccagcgcg ttacggaatt cttcgtgcat gatgcggcga gcgaggcgcg agaaaatgcg
     8881 catgtgctga tcgcccgcag cgtgtttgtt cagggttaac ataatgatga attgcgcttc
     8941 atcatcgccc cagcgcaccg gcgcttgcag acgcgccacg ctgatggtgg attgctcaat
     9001 gtgttctgat ttgctgtgtg gaatggcaaa actaaagccc agaccggtag agaaaacggc
     9061 ctcgcgcgcc cataagtcgg cttccagttt acgcggatag cggcagcggc ccgccagcag
     9121 caggttatcg gtcatgcctt tgagcacttc ttctttgctg cgccagtcac tttccagcgt
     9181 gatgcactcg gcggtgacca gcggtgcgtc ctgttgggtc atgcggaatt gcgccagcag
     9241 gtgttccact tccagcgaag tacggcaggc cattgcctgg ttgagcaact tgcggcactc
     9301 acggctatca agttgcgcca tccgcgcttt cgccgccgga attgatggtg cgctcatgct
     9361 gagttcatcc agccctaagc cgaccagcaa cggcagcacg gaacctttcg ctcccagctc
     9421 accgcacaga ccaatccatt taccctggcg atgcaccgcc tgcacgtcgt aatcgagcgc
     9481 ccgcaagaat gccggattca ggctgttgta gtgacgagta accttagcgt tatcgcgatc
     9541 cactgccagc agatattgcg tcaggtcatt actaccaata ctaaagaaat caatctcttc
     9601 gcagcattga tcgatgatga acatcaccga cggcacttcc agcataatgc cgagctggat
     9661 cttctcatca aacggaatgt gttcgttacg tagttgctgt ttggcttctg ccagtttttc
     9721 tttcacccat aagatctctt ccattgagga gatcatcggg atcatgattt tcaggctgcc
     9781 gtgagcggag gcgcgcagga tcgagcgtag ttgtgtggta aataacgacg cgtattcttc
     9841 ataaatacgc acggcgcgat aaccgaggaa cgggtttgcc tctgcgggaa tgtttagata
     9901 atcaacgggt ttgtcgccgc caatgtccat agtgcgcaca ataatgctgc gtccgttggc
     9961 agactccagc gcctgacaaa aaatgttgta cagctcgctt tcgcccggtg cactggtgcg
    10021 atccatatag agcatttcag tgcggaacaa accaacgcct tccgcaccat tgccgaatgc
    10081 ggcctgcgct tccacggagt gagcgatgtt agcggcaatt tcaatgcgga taccgtcagc
    10141 ggtacgggct tgttgggtca gctagacacg ctgttgctca cgcagggcgt cctgtacgcg
    10201 ggcttcttgc tgataataac gagctacggc ttctcctggc tcaaccacaa tcgccccggc
    10261 gttgccgtcg atataaatcg tttgatgctg ccacggagtg agggcatcaa tatccacacc
    10321 aaccagcgtc ggaatgttga acgaacgggc aaggatcacc gtatgtgagg tggtgccgcc
    10381 gcttttcagt aacaatcctt tgaggtgatt tttatccagt tcgaggaact ggctgggggt
    10441 cagttcatca gccatacaaa tggcggactg cgtcagtttg cccggtgccg ggaagcgttg
    10501 ctcaccgtag atttgctgga gtaactggaa gcagacgtcg cgtacgtcca gggcacgttc
    10561 ttgcaggtat ctgctgctgg aacgggcaaa ctcttcgcaa aagtgattcg cactggcgac
    10621 aatcgcttcg gcgcagctta atccggcgct gacgcctgcc agtaaatgtt cgtgcaggga
    10681 agtatcgcca gccagggatc ggtgcgcttc cagaatcgcg ctggtagcac cgtcgctatc
    10741 cagcagacga aactcaatgt ttttcagtac cagcgtcagg ccgttttcca gtgcggattg
    10801 ctcggcgtca acgtctttgg ctgcgggaag attactcagc gcattgagat ctaaagaaga
    10861 gatcggcgtc agaatgccgc ctgcactacc gctgcacacg gtgcgggcgc ggataatttg
    10921 cggattcaga ttggtcagtg aaaccggcag tggttccagt tcgtcagatt taacttccgc
    10981 cagcggcgca tcgcagtggg ggaattcatc gcgcagccat tggcttaaac gctggtgccc
    11041 ttcctgttcg tcggccccgg aaatcagtag ctggcagtta tcacccacca gcgtatcggt
    11101 gccaatcagc gccagagcgc ttttggcgtt gcctttacgg tcagtacgca ggttatgcca
    11161 ctcaatttga gatgaaaaag tattacacag cgtttcaacg tggcttgccg gacgcgcatg
    11221 tacgccgtta ggtagctcac aaataaattc cacaatcagg gccattgcct ttctcccggt
    11281 acggttttct gtttacagga taagagcgca cggtaacggc ctgccatgtg acaaatctgc
    11341 caaaagctgg acaaatgtaa tgtaaccgtc aatttgcgac gcgtctcaca agacgccgtt
    11401 ttgcggcatg cttccggatt atcgcaagtt atgaggcgga tcgcattttt gtactgatat
    11461 tacaaaagtc cagtaaatgg cctttttatc cactgtttgc tccgcctgcg attgcctatt
    11521 gtttcctcca tcaaaaatat ggacatgggc ttttgcaagc ccgggagcag cttatgaatg
    11581 agttggtgca gatcctgaaa aatacccgtc agcatttaat gacgggcgtt tcacacatga
    11641 ttcccttcgt ggtatcgggc ggtattttgc tggcggtttc cgtcatgttg tatggcaaag
    11701 gcgcagtgcc ggatgccgta gccgatccga atttgaaaaa actgtttgat atcggcgttg
    11761 caggtttgac gctgatggtg cctttcctcg ccgcttacat tggttactcc attgcagagc
    11821 gttctgcgct ggctccgtgc gctatcggtg cctgggttgg taacagcttt ggtgcgggct
    11881 tctttggtgc gctgatcgcc gggattatcg gcggcatcgt ggtgcattac ctgaagaaaa
    11941 ttccggtgca taaagttctg cgctcggtga tgcccatctt cattattcct atcatcggca
    12001 cactgattac cgcaggcatc atgatgtggg gcttgggcga gcctgtaggg gcgttgacca
    12061 acagcctgac tcagtggctt caggggatgc agcagggcag cattgttatg ctggcggtga
    12121 tcatggggct gatgctggcg ttcgatatgg gcggtccggt taacaaagtg gcctatgcct
    12181 tcatgctgat ttgcgttgct cagggtgttt ataccgtggt ggccattgct gccgtgggta
    12241 tttgtatccc gccgctgggg atggggctgg cgacgctgat tggtcgtaaa aatttctccg
    12301 cagaagagcg cgaaaccggc aaagcggcac tggtgatggg ctgtgttggc gttactgaag
    12361 gggcgattcc tttcgccgct gccgatccgc tgcgtgtcat tccttccatt atggtcggtt
    12421 cagtttgtgg tgcagtaact gcggcgctgg tcggtgcgca gtgctatgca ggctggggtg
    12481 gtctgattgt gctgccggtg gttgaaggta agttgggtta tatcgcagca gtggctgtcg
    12541 gagcagtggt gacggctgtt tgtgtgaacg tgctgaaaag tctggcgcgt aaaaatgggt
    12601 cttcgactga tgaaaaagaa gacgacctgg atttggattt tgaaattaat taattgagga
    12661 acgagccatg acgaaaatta ttgcagtaac cgcatgtcct tcaggtgttg cccataccta
    12721 tatggcagca gaggcgctgg aaagtgccgc gaaagcgaaa ggctgggaag tgaaagtaga
    12781 aacccagggg tcaattggtc tggaaaatga actgactgcg gaagacgtgg cgagcgctga
    12841 tatggtgatt ctgaccaaag atatcggcat caagtttgaa gagcgttttg ctggcaagac
    12901 catcgtgcgc gtcaacatca gcgatgcagt aaaacgtgct gacgccatta tgagcaaaat
    12961 tgaagctcac ctggcgcaaa ccgcttaagt tcttctcccg ctcgcaaggg cgggttcgct
    13021 ttcccacagg agttcctcat gacgaatcgt atctctcgcc tcaaaactgc actgtttgcc
    13081 aatacccgtg aaatctcgct ggagcgggcg ctgctttata ccgccagcca tcggcaaacc
    13141 gaaggcgaac cggtgatatt gcgccgggcg aaagcaacag cgtatatcct tgaacatgtt
    13201 gaaatttcga ttcgtgatga agaactgatt gccggtaacc gcaccgtaaa accgcgcgcc
    13261 gggattatgt cgccggaaat ggacccttac tggctgctga aagagctgga tcaattcccg
    13321 acgcgtccgc aggaccgctt tgctatcagc gaagaagata aacgtatcta ccgtgaagag
    13381 ttgttcccgt actgggaaaa acgttcgatg aaagatttca tcaacgggca gatgacagat
    13441 gaagtaaaag ccgcgaccaa cacgcagatt ttcagcatca accagacgga taaaggccag
    13501 gggcacatta ttattgatta cccacgcctg ttgaatcacg gtctgggtga gctggtggca
    13561 cagatgcagc aacattgtca gcaacagccg gagaatcact tttatcaggc cgcgttgtta
    13621 ctgctggaag cctcgcagaa acacattttg cgttacgccg aactggcgga aacgatggcg
    13681 gcaaactgca cagatgccca gcgtcgcgaa gagctgctga ctattgcgga gatctcccgc
    13741 cataacgcgc aacataagcc gcagacgttc tggcaggcgt gccagttatt ctggtacatg
    13801 aacatcattc tgcaatacga atccaacgcc agttcgctgt ctttagggcg cttcgaccag
    13861 tatatgttgc cgttctatca ggcatcttta acccagggcg aagatccggc gttcctgaaa
    13921 gaactgctcg aatctttatg ggtgaaatgc aacgacatcg tgctgttgcg ctccaccagt
    13981 agcgcgcgtt atttcgccgg tttcccgacc ggctataccg cactgctcgg cgggttaacc
    14041 gagaacggac gtagcgcggt gaacgtgctt tcgttccttt gccttgacgc ctatcaaagc
    14101 gtgcaattac cgcaaccgaa cctcggcgtg cgcactaacg ccttgatcga cacgccgttc
    14161 ctgatgaaaa ccgccgaaac cattcgcctc ggcaccggta ttccgcaaat ctttaacgat
    14221 gaagtggtgg tgccagcgtt cctcaaccgt ggcgtttcgc tggaagatgc gcgcgactat
    14281 tccgtagtgg gctgtgtgga attatctatt cccggcagaa cctacggctt gcatgacatc
    14341 gcgatgttta atctgctgaa agtgatggaa atctgcctgc atgaaaatga aggcaatgct
    14401 gcgctgactt atgaaggttt actggagcag atccgcgcca agatcagcca ctacatcacc
    14461 ctgatggttg agggcagcaa tatttgtgat atcggccatc gcgactgggc acctgtaccg
    14521 ctgctctcgt cttttatcag cgattgtctg gaaaaaggtc gcgatattac cgatggcggc
    14581 gcgcgttata acttctccgg cgtacaggga attggtatcg ccaacctgag cgattctctc
    14641 catgcgttga aagggatggt ttttgagcaa cagcgtttaa gttttgacga attgctgtcg
    14701 gtattaaaag ccaactttgc aacgccagaa ggcgaaaaag tccgcgctcg cttaattaac
    14761 cgctttgaga aatacggtaa cgatatcgac gaggtggata acattagcgc cgaactgttg
    14821 cgccactact gcaaagaagt ggaaaaatac cagaacccgc gcggcggtta cttcacgccg
    14881 ggatcgtata ccgtttctgc tcacgttccg ttgggatcgg tggttggcgc gacgccagac
    14941 ggtcgttttg ccggggaaca actggcagac ggcggcttgt cacctatgct gggtcaggac
    15001 gcacaagggc caacggcggt actgaagtca gtcagtaagc tcgataatac gctgctgtct
    15061 aacggtacgt tgctgaacgt gaaattcact ccggcgaccc tggaaggtga agcgggatta
    15121 cgcaaactgg ccgacttctt acgggtgttt acccagctta agttgcagca tattcagttt
    15181 aacgtggtga acgccgacac gttgcgggaa gcgcaacagc gcccacaaga ttatgccggg
    15241 ctggtggtgc gcgttgccgg atacagcgcc ttctttgtcg aactgtcgaa ggagatccag
    15301 gatgacatca tccgccggac agcgcatcag ctgtaacgtt gtggaaacgc gccgcgatga
    15361 tgtggcgcgc attttcaaca ttcagcgtta ttcactgaat gacggcgagg gcattcgtac
    15421 ggtggtcttt tttaaaggct gtccgcatct ttgcccgtgg tgtgctaatc cggagtcgat
    15481 ctccggcaaa atccagacgg tacgcagaga ggcgaaatgt ctgcactgtg cgaaatgttt
    15541 gcgtgatgcg gatgaatgcc cctccggggc gtttgaacgg attggtcgcg atatcagcct
    15601 tgacgctctg gaacgggaag tgatgaaaga tgacattttc tttcgcacgt ccggcggcgg
    15661 cgtcacgctt tctggcggcg aagtgttaat gcaggcggag tttgctaccc gttttttaca
    15721 gcgactgcgg ctgtggggtg tctcatgcgc cattgaaact gccggagacg cgccagcaag
    15781 caagctgtta ccgctggcga aattgtgcga tgaagtgctg ttcgatttaa aaattatgga
    15841 cgcgactcag gcgcgggatg tggtgaagat gaacctgcca cgcgtgctgg agaatctgcg
    15901 tttgctggtg agtgagggtg tcaacgtgat cccgcgttta ccgttgatcc ctggtttcac
    15961 gctcagccgg gagaatatgc agcaggcgct ggatgtactg accccgctga aaatcaggca
    16021 gatccatctg ttaccgtttc atcagtacgg cgaaccgaaa taccgcctgc tggggaaaac
    16081 atggtcgatg aaagaggtgc ctgcgccgtc gtcagccgat gtgacaacga tgcgcgaaat
    16141 ggcagaacgg gccggatttc aggttaccgt gggaggttaa aatggcatac ctggtggcag
    16201 taaccgcctg cgtaagcggc gtggcgcata cttatatggc ggcggaacgg ctggaaaagt
    16261 tgtgccagtt agagaagtgg ggcgtcagca ttgaaactca gggcgcgctg ggaacggaga
    16321 atcgtttagc ggacgaggat atccgtcggg cggatgttgc tctgttgatt acggatatcg
    16381 agcttgccgg tgccgagcga tttgaacatt gccgctatgt gcaatgcagc atctacgcat
    16441 tcctgcgtga gccgcagcgg gtaatgagcg cggtgcgcaa agtgctttct gcgccgcagc
    16501 aaacccatct tattctggag tagtcggttt ttctgtcagt tggctgtggt actgccggcg
    16561 atattccgac ggcgagtgtt cggtgttttt acgaaacaga cggcagaagt agttgctgtc
    16621 gacaaagccg caggcgtgcg ccacttcttt taccttcagg tcatagcctt tcaataacgt
    16681 cttagcgtgc tccagtcgcg tgtgattcag gtattcgtta aaaccaatgg cccccgtttt
    16741 ttgaaacagg tgcgagagat agtttggtga aatatagaat gcctgtgcga cagattcgcg
    16801 ggtaagcgcg gaggcatagc gttcgtcgat ataatcgcga atagcttcaa atagtgcctg
    16861 gctgcgtgag gcggtctgga tttggctgcc gagcaaatcg cggcagtggc taagcaggct
    16921 ggctacgata agccttgccg tttgctgctc ctgcggctgc atttgcattt cattgagcgt
    16981 ttgtagcaga aaagaaccta tgcgtgggcc gcgtcgggcg acgtgttgct tcgccagatt
    17041 ttgatattgt ttgccatccc attgcacgac gctgaagccg agttgctgtt tgccaaacag
    17101 cacgctaaag gtggtagcgg gtgttttcca ttgtgggaaa ttccagccac cagcggcaac
    17161 gtacagcaca tcgccgggaa ctaacgttgc tccagcgccg gtatcaacaa actcaccttc
    17221 cagcacaatt tccagccgtg gaaaatcgac ctgatacgca agatcgggca ccgggccgtt
    17281 actgctggca aagtaaatct ggcgcaggga cagcgggccg ttgatcaggc gggagagcag
    17341 atagctgacg tcgtgataca tgttattccc ttaggtatgt agacgggaaa acagtaactg
    17401 gcattattaa aaaaggccac ctgaaaagtg tggcctgagg ggagttcgat gtaaacattg
    17461 cggcagtatc gcatccggca aatagcgcca gacgcgatac aaaggtcatc tactgattac
    17521 ccacctgatc gccatacggc agagtgtcgt aatcgatcag cgcgtttttc ttgtacgggt
    17581 taccaatcca gcgggtagtt tctttgaact gcggattcac cactgaacgg gttggatcgt
    17641 aaccgtcgta agataagccc gccaaatctg accaggtgtg gatcagttcc gccaggctgt
    17701 atttacgatc gacatcctgt gagaaatcac ggggatgagt cgcttgccat ttttccgacg
    17761 tccacagcag gaacggaatg gtgtacatgt ggcgcgtcgg gttgtcttcg ttacgcccct
    17821 gagttttgtg cggcggcgtg tcgtaaacct cttcaccgtg gtcagagaag taaaccagga
    17881 atccgttcgg gtctgccgct ttaaagtctt taatcaggct ggcaaccaca tgatcgttat
    17941 acaggttagc gttgtcataa tcgttatatg attccagctc ttctgcgctt aatcctggcg
    18001 gaacatgatc ggtattgcca tcaaacttgc cctgattttc cgggtagcgg tatttgtatt
    18061 tgatatgcgt acccagcaga tgaacgatga tcagtttctt cggcgcaggg tcattcagca
    18121 catcctggaa cggcttcagc acgttggtgt cgtattcacg cgcactctgc gtacgttgct
    18181 ggttcatgta gtactgcttg tcggtctggc gcgaaaatac cgtcagcatg gtattgcggg
    18241 cggtcatcgt ctgctggttg gtgatccaga aggttttata acccgcctgt ttcatcatgt
    18301 tcatcagcga cggctgcgtc agatacagat ccgggttctt ttcattggca aaggtcagcg
    18361 cctgttgcag gatttcaatg gtgtacggac gagacgtaac tacgttatta aacacggtca
    18421 gattcggatc ggttttatgc agcgcatcca gctccggcgt ggtttcacgc ggataaccgt
    18481 acagactcat gcgtccgcgc tgggtcgact cgccaatcac cagcactaaa gtgcgcggtt
    18541 cgttacccga ttcatctttg aaattagcca gtggcggcaa ggcattattt tcattcagta
    18601 actttgttag cgagtttagt tgctgacgat actgataata gccggtcagg aattgccacg
    18661 gtgcggcagg ctccatacgc gaggctaggt tatccaacgt tttctcaaac ggcttgtttt
    18721 tgataaacgt attcatggcg atcggatgca gaatcaagcc ataaagcagg gcaaaagaga
    18781 caacataacg ccacggcttt ggaatataga ccgggcgcag gcgtgtccac agcagaactg
    18841 ccaccgccgt ataggccagc gcgataagca caattttcag gctgaaatac tggcttaaat
    18901 actcgctggc ttcgttggtg ttggtttcga acatcacaaa cagaacgctc tgcgagaact
    18961 cctgcccgta gatgacgtag tagcacagcg ccgccagaga ggccgtccat agcaccacgc
    19021 cgatcactgc ggcaataatt ttaatccgct tcggaaagag gaataccggg atcaaccaca
    19081 gcgagctgaa taacagcgag tcgcgaatgc cgttggtgcc actataacca ctgatgtaaa
    19141 taatggcctg tagcagagta gagaaaaacc aaaagtagag cagtgcccaa cccagggctt
    19201 tccagctaga aaggggttta gcctggactt ctgtggaatg catagtaaga acctgtcttg
    19261 aaaaaaatat cgccgaatat aacgacaatt ccttaaggat atctgaaggt atattcagaa
    19321 tttgaataaa atgcagacag aaatatattg aaaacgaggg tgttagaaca gaagtatttc
    19381 agaaaaccct cgcgcaaaag cacgagggtt tgcagaagag gaagattagc cggtattacg
    19441 catacctgcc gcaatcccgg caatagtgac cattaacgct tgttcgacgc gaggatccgg
    19501 ttcctggcct tctttttctg cctggcggga gcggtgcagc aactcggcct gcaatacgtt
    19561 cagcgggtcg gtgtaaatat tccgtagctg aatagactct gcaatccacg gcagatcggc
    19621 catcagatgg gaatcgttgg caatcgccag caccactttg atgtcttctt cttgcaggtt
    19681 gcgtaactct ttacctaacg gccacagtgc tttgtctacc agacgttgat catagtattc
    19741 cgccaaccac aggtctgctt tggcgaagac catctccagc atgccgagac gcgtcgagaa
    19801 gaatggccaa tcgcggcaca tggcttccag ctcgctctgt ttgccatctt cgaccacttt
    19861 ttgcagcgcc gtacctgcac ccagccaggc ggggagcatc aggcggtttt gcgtccaggc
    19921 aaaaatccac ggtatggcgc gcagcgactc gacgccgccg gttgggcgac gtttcgccgg
    19981 acgtgaaccc aacggcagtt tgcccaattc ttgttccggc gtagcggagc ggaagtaagg
    20041 cacaaaatct ttgttttcac gtacgtagcc acggtagaga tcgcaggaga tgactgacag
    20101 ttcatccata atgcgacgcc agctctcttt cggctcaggt ggtggcagca ggttggcttc
    20161 cagaatcgcc ccggtgtaaa gcgacaggct gctgacggtg atttctggca gaccatattt
    20221 aaagcggatc atctcgccct gttcagtcac gcgcaggcca cctttcaggc ttcctggcgg
    20281 ttgtgacagc agcgccgcat gggcaggtgc gccgccgcga ccaatggaac cgccgcgacc
    20341 gtggaacaac gtcagctcaa tacccgcttt ttcgcaggtt ttgattaatg catcctgtgc
    20401 ctgatattgc gcccaggaag ctgccatcac gcccgcatct tttgctgagt cggaatagcc
    20461 aatcatcacc atctgtttgc cctgaatcag gccgcgatac cagtcgatat tgagcagctg
    20521 ggtcatgaca tcgttggcgt tgttcaggtc atcgagggtt tcaaacagcg gagcaaccgg
    20581 catcgcaaac ccgatacccg cttctttcag cagcaggtgg acagccagta cgtcggacgg
    20641 cgttttcgcc atcgagatca cgtaggcggc aatggagcct tgcggcgctt cggcaatcac
    20701 ctggcaggta tcgagcactt cgcgcgtttc ggcgcttggt tgccagttgc gcggcagaag
    20761 cggacgtttg gagttcagtt cgcggatcag gaacgcctgt ttgtcggcct ctgaccagct
    20821 ttcgtagtcg ccgataccga ggtagcgggt cagctcgccc agcgcttcgg tatggcgggt
    20881 gctctcctga cggatatcaa tacggaccag cggtacgccg aaacatttca cgcggcgcag
    20941 ggtgtcgagc agatcgccgt tggcgataat acccatgcca cacgcctgaa gtgactggta
    21001 gcaagcgtag agcggttccc acagttcttc gttttgtgtc agcagccctt ctggttttgg
    21061 cagttcttcg cctttcaggc gcgcttccag ccatgcctgt gtcgccatca ggcgagaacg
    21121 caggtttttc atcagatagc gatacggttc tgcggcacct tcttcgccaa ccagcgccag
    21181 cagttcaggg gtcgcttcaa ccatcgacag ttcagaaacc agaacctgaa tatctttcag
    21241 gaacagatcg gtggctttcc agcggctgag cagcaggacg tggcgggtga tatcggcagt
    21301 gacgttcggg ttgccgtcgc ggtcaccgcc catccacgag gtaaaacgta ccggaacaaa
    21361 ttcgacgggc agtttgtagc cgagattctc ttccagttgt tcgttcagtt cgcgcaggta
    21421 attcggtacg ccttgccaca ggctgttttc tactacggca aagccccatt tggcttcatc
    21481 taccgggctt ggacgcagct tacggatttc atcggtatgc catgactggg cgatcaactg
    21541 gcgcaggcga cgcatcagct ggttgcgttc gtagtcagcg atatctttgt tatcgagctg
    21601 ttttaaacag gcgttcactt ccaccatttt gtggatcagt gtacgacggg taatttcggt
    21661 tgggtgagcc gtgaggacca gttccagcga cagcgattcc actgcttttt tgatggtgtc
    21721 ttcgctcagt tccggctggt ttttcagttt acgcagggtg cgggcgatca cttccgggtt
    21781 gctggcagct tcgcctttcg gcgaaatgct gtggtattgc tcggcggtgt tggccaggtt
    21841 caggaactga ctaaacgcac gcgcaacggg cagcagctcg tcgttcgaca aattttgtaa
    21901 ggtggtgagc aactcctggc ggttagcatc attgccagcg cgtgaagatt tcgacaactt
    21961 acggatagtt tctacgcgtt caagaatgtg ttctcccaac gcatccttga tggtttctcc
    22021 cagcactttg ccgagcatac tgacattact acgcaatgcg gaatattgtt cgttcatatt
    22081 accccagaca ccccatctta tcgtttgata gccctgtatc cttcacgtcg cattggcgcg
    22141 aatatgctcg ggctttgctt ttcgtcgtct tttataaagc cacgtaaaag cggtgacgtc
    22201 aaatgctgcg aaatcgcttc agcaaacgaa taaatagcag gaatttacgc cattaaattc
    22261 acgacgcttt aaataagcgt aacttatgga aatgttaaaa aatcgccata cataacacca
    22321 aaggtgtagg tcggataaga tgcgcaagca tcgcatccga cattattgcg gcaccggagt
    22381 ttggcaacgg tgccggatgc ggcgcgagcg ccttatccgg cctacgtttt aatgccagca
    22441 aaaatggtga attacctgga taatcaactc acgggtgggt ttaataaacc gcgtctccag
    22501 atattcatca ggctgatgag cttgattgat ggaacccgga ccgagcacca gcgtcgggca
    22561 taacgtttga ataaacggcg cttcggtaca gtaattcacc acttcggttt ttgctccgag
    22621 caatttctca accacttcaa ccagttgatg attcggtggg cattcatagc cagggatcgg
    22681 cggatgcagc tcgtcgaccg tcagacgacc cggccagcgt tcgctcaccg gagccaatgc
    22741 atcgttgagc aaaccattaa gttcattgag tgtcatgcca ggctgcggac gaatatccat
    22801 atgcaactca cagcaagcgc aaatacggtt agaagcgtcg ccaccgtgaa tatgcccgag
    22861 gttgagcgta gggtatggca cggtaaatgc ttcgtagtga taacgttctt tcaggttatc
    22921 gcgcaattgc aaaatatgcc cgatggcgtc gtgcattagt tcgatagcgt taattccgcg
    22981 tgctggatcg ctggagtgcc ccgactggcc ctgaatacgg atggcgttag agatatgacc
    23041 tttatgtgcg cgtaccggtt gtagtgacgt cggttcgcca atgatggcgc aatccgggcg
    23101 cagggcggta gtttcggcaa aataacgcgc tccggccata ctggtttctt catcagcagt
    23161 cgccagaatg tagagcggtt ttttcagttt cgtgacgtcg acatcgcgta gcgcatcaag
    23221 gataaacgca aaaaagcctt tcatgtcggc ggtgcctaag ccgtaaagct tgccgtcatg
    23281 ctccgtcagt gtaaacggat cgcgcgtcca gcgaccgtca tcaaatggca ccgtatcggt
    23341 atgccccgcc agcaacaagc cgccagcccc ctgtccgtaa ctggccagca tattaaattt
    23401 gttgcgagtt cctggaacag gctgcacttc cacattgaag cccaaatctt taaaccagtc
    23461 cgccagcaga gtgattaaat ctgcattgct ttgatcgagt gcctcttccg tagcgcttat
    23521 tgaaggtgtg gcaatcagag cgcggtaaat ctcgataaat ggcggtaatt tgtttttcat
    23581 tgttgacaca cctctggtca tgatagtatc aatattcatg cagtatttat gaataaaaat
    23641 acactaacgt tgagcgtaat aaaacccacc agccgtaagg tgaatgtttt acgtttaacc
    23701 tggcaaccag acataagaag gtgaatagcc ccgatgttga atacgctgat tgtgggtgcc
    23761 agcggctacg ctggcgcaga gctagtgacc tatgtaaatc gccatccgca tatgaacata
    23821 accgctttga ctgtttcagc gcaaagcaat gatgcgggaa agttaatctc cgatttgcat
    23881 ccgcagctaa aaggcatcgt tgagctgccg ttgcagccga tgtcggatat cagcgagttt
    23941 agcccagggg tggacgtagt gtttctcgcc accgcccatg aagttagcca cgatttagcg
    24001 ccgcagtttc ttgaagcggg ctgcgtggtg ttcgaccttt ccggcgcgtt tcgtgttaac
    24061 gacgccacct tctatgaaaa atattacggc tttacccatc aatacccgga actgctggaa
    24121 caggcagcct acggtctggc ggagtggtgc ggtaataaat taaaagaagc gaatttgatt
    24181 gcggtgccag gctgttatcc gacggcggca cagctggcgc tgaaaccgtt gattgatgcc
    24241 gatcttcttg acctcaatca gtggccggtg atcaacgcca ccagcggcgt gagcggtgca
    24301 ggacgtaagg cagcgatttc aaatagtttt tgtgaagtta gcctgcaacc gtatggcgtc
    24361 tttactcatc gccatcaacc agagatcgcc acacacctcg gtgctgacgt tatcttcacc
    24421 ccacatctgg gcaatttccc gcgcggcatt ctcgaaacca ttacctgccg cctgaaatcg
    24481 ggtgtgaccc aggcgcaagt cgcgcaagcg ttacagcagg cgtatgccca taaaccgctg
    24541 gtgcggctgt atgacaaagg cgttccggcg ctgaaaaatg tcgttgggct gccattttgc
    24601 gatatcgggt ttgccgttca gggcgagcat ctgattattg tggcgaccga agacaactta
    24661 ctgaaaggcg cggcggcaca agcggtacag tgcgccaata ttcgtttcgg ctatgcggaa
    24721 acgcagtctc ttatttaagg gtgcaatgat gaatccatta attatcaaac tgggcggcgt
    24781 actgctggat agtgaagagg cgctggaacg tctgtttagc gcattggtga attatcgtga
    24841 gtcacatcag cgtccgctgg tgattgtgca cggcggcggt tgcgtggtgg atgagctgat
    24901 gaaagggctg aatctgccgg tgaaaaagaa aaacggcctg cgggtgacgc ctgctgatca
    24961 gatagacatt atcaccggag cactggcggg aacggcaaat aaaaccctgt tggcatgggc
    25021 gaagaaacat cagattgcgg ccgtaggttt gtttctcggt gacggcgaca gcgtcaaagt
    25081 gacccagctt gatgaagagt taggtcatgt tggactggcg cagccaggtt cgcctaagct
    25141 tatcaactcc ttgctggaga acggttatct gccggtggtc agctccattg gcgtaacaga
    25201 cgaagggcaa ctgatgaacg tcaatgccga ccaggcggca acggcgctgg cggcaacgct
    25261 gggcgcggat ctgattttgc tctccgacgt cagcggcatt ctcgacggca aagggcaacg
    25321 cattgccgaa atgaccgccg cgaaagcaga acaactgatt gagcagggca ttattactga
    25381 cggcatgata gtgaaagtga acgcggcgct ggatgcggcc cgcacgctgg gccgtccggt
    25441 agatatcgct tcttggcgtt atgcggagca gcttccggca ctgtttaacg gtatgccgat
    25501 gggtacgcgg attttagctt aagttttgtt agccggaggc gcagctttcc ggcattgaat
    25561 ttcaaaataa ggaaacagag ttatggcact ttggggcggg cgttttaccc aggcagcaga
    25621 tcaacggttc aaacaattca acgactcact gcgctttgat taccgtctgg cggagcagga
    25681 tattgttggc tctgtggcct ggtccaaagc cttggtaacg gtcggcgtgt taaccgcaga
    25741 agagcaggcg caactggaag aggcgctgaa cgtgttgctg gaagatgttc gcgccaggcc
    25801 acaacaaatc cttgaaagcg acgccgaaga tatccatagc tgggtggaag gcaaactgat
    25861 cgacaaagtg ggccagttag gcaaaaagct gcataccggg cgtagccgta atgatcaggt
    25921 agcgactgac ctgaaactgt ggtgcaaaga taccgttagc gagttactga cggctaaccg
    25981 gcagctgcaa tcggcgctgg tggaaaccgc acaaaacaat caggacgcgg taatgccagg
    26041 ttacactcac ctgcaacgcg cccagccggt gacgttcgcg cactggtgcc tggcctatgt
    26101 tgagatgctg gcgcgtgatg aaagccgttt gcaggatgcg cttaagcgtc tggatgtcag
    26161 cccattaggc tgtggcgcgc tggcgggaac ggcctatgaa atcgaccgtg aacagttagc
    26221 aggctggctg ggctttgctt cggcgacccg taacagtctg gacagcgttt ctgaccgtga
    26281 ccatgtgttg gaactgcttt ctgctgccgc tatcggcatg gtgcatctgt cgcgttttgc
    26341 tgaagatctg attttcttta acaccggcga agcggggttt gtggagcttt cggaccgcgt
    26401 gacttccggt tcatcattaa tgccgcagaa gaaaaacccg gatgcgctgg agctgattcg
    26461 cggtaaatgc ggccgggtgc agggtgcgtt aaccggcatg atgatgacgc tgaaaggttt
    26521 gccgctggct tacaacaaag atatgcagga agacaaagaa ggtctgttcg acgcgctcga
    26581 tacctggctg gactgcctgc atatggcggc gctggtactg gacggcattc aggtgaaacg
    26641 tccgcgttgt caggaagcgg cgcagcaggg gtatgcgaac gccactgaac tggcggatta
    26701 cctggtggcg aaaggcgtac ctttccgcga ggcgcaccat attgtgggtg aagcggtggt
    26761 ggaagccatt cgtcagggca aaccgctgga agatctgcca ctcagtgagt tgcagaaatt
    26821 cagtcaggtg attggcgaag atgtctatcc gattctgtcg ctacaatcgt gcctcgaaaa
    26881 gcgtgcggca aaaggcggcg tctcaccgca gcaggtggcg caggcgattg cttttgcgca
    26941 ggctcggtta gagtaatttg tttatattgc tcccctgaaa atggggagtt taccacgcct
    27001 acaaagtgcg acacttcgta ggcggataag gcgtttacgc cgcatcaggc agaaatttgt
    27061 cagcattc
//
LOCUS       DAZSNM010000031        26173 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZSNM010000031 DAZSNM010000000
VERSION     DAZSNM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26173
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.031"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            246..353
                     /locus_tag="ACHW42_001148"
     CDS             246..353
                     /locus_tag="ACHW42_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR8943052.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(440..2119)
                     /gene="bcsG"
                     /locus_tag="ACHW42_001149"
     CDS             complement(440..2119)
                     /gene="bcsG"
                     /locus_tag="ACHW42_001149"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR8943053.1"
                     /translation="MTQFTQNTAMPSSLWQYWSGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2116..2307)
                     /gene="bcsF"
                     /locus_tag="ACHW42_001150"
     CDS             complement(2116..2307)
                     /gene="bcsF"
                     /locus_tag="ACHW42_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR8943054.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2304..3875)
                     /gene="bcsE"
                     /locus_tag="ACHW42_001151"
     CDS             complement(2304..3875)
                     /gene="bcsE"
                     /locus_tag="ACHW42_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR8943055.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            4148..4336
                     /gene="bcsR"
                     /locus_tag="ACHW42_001152"
     CDS             4148..4336
                     /gene="bcsR"
                     /locus_tag="ACHW42_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR8943056.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4348..5100
                     /gene="bcsQ"
                     /locus_tag="ACHW42_001153"
     CDS             4348..5100
                     /gene="bcsQ"
                     /locus_tag="ACHW42_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR8943057.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            5097..7714
                     /gene="bcsA"
                     /locus_tag="ACHW42_001154"
                     /pseudo
     CDS             5097..7714
                     /gene="bcsA"
                     /locus_tag="ACHW42_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7725..10064
                     /gene="bcsB"
                     /locus_tag="ACHW42_001155"
     CDS             7725..10064
                     /gene="bcsB"
                     /locus_tag="ACHW42_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR8943058.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            10071..11177
                     /gene="bcsZ"
                     /locus_tag="ACHW42_001156"
     CDS             10071..11177
                     /gene="bcsZ"
                     /locus_tag="ACHW42_001156"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR8943059.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11372..11761
                     /locus_tag="ACHW42_001157"
     CDS             11372..11761
                     /locus_tag="ACHW42_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HFR8943060.1"
                     /translation="MISNAACKTTVWHSRQRYWKTRANGRRRQHFSGNDWRWAPAAYG
                     LLTDWQEEAARQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQVKDYAPSHFL
                     LYVRYSAAGWQGDMDLPPQPLIPYADW"
     gene            11843..13831
                     /gene="hmsP"
                     /locus_tag="ACHW42_001158"
     CDS             11843..13831
                     /gene="hmsP"
                     /locus_tag="ACHW42_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR8943061.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            14020..15306
                     /gene="dctA"
                     /locus_tag="ACHW42_001159"
     CDS             14020..15306
                     /gene="dctA"
                     /locus_tag="ACHW42_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR8943062.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15527..17023
                     /locus_tag="ACHW42_001160"
     CDS             15527..17023
                     /locus_tag="ACHW42_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR8943063.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(17119..18048)
                     /gene="kdgK"
                     /locus_tag="ACHW42_001161"
     CDS             complement(17119..18048)
                     /gene="kdgK"
                     /locus_tag="ACHW42_001161"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR8943064.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            18280..19047
                     /gene="pdeH"
                     /locus_tag="ACHW42_001162"
     CDS             18280..19047
                     /gene="pdeH"
                     /locus_tag="ACHW42_001162"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR8943065.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            19117..21177
                     /locus_tag="ACHW42_001163"
     CDS             19117..21177
                     /locus_tag="ACHW42_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR8943066.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21359..22681)
                     /locus_tag="ACHW42_001164"
     CDS             complement(21359..22681)
                     /locus_tag="ACHW42_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8943067.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(23091..24104)
                     /gene="yhjD"
                     /locus_tag="ACHW42_001165"
     CDS             complement(23091..24104)
                     /gene="yhjD"
                     /locus_tag="ACHW42_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR8943068.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(24153..25052)
                     /locus_tag="ACHW42_001166"
     CDS             complement(24153..25052)
                     /locus_tag="ACHW42_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8943069.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            25572..>26173
                     /locus_tag="ACHW42_001167"
     CDS             25572..>26173
                     /locus_tag="ACHW42_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR8943070.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6246 a         6572 c         7129 g         6226 t
ORIGIN      
        1 cagcacagag tgaagacgtg acggattccg gaacagaaaa acagtcgtag aatcagcggc
       61 aagccggaaa ggttccggtg aggcgcaatg ttgcgggggc tttatccctg gtggcattgg
      121 ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca tcacgggggc
      181 taatcttgaa cccaaacaac tcaagaatag ccgcgaaacg ttggcattac aacacaggcg
      241 gctatatgac gctcgcagaa ctgggcatgg ccttctggca tgatttagcg gctccggtca
      301 ttgctggcat tcttgccagt atgatcgtga actggctgaa taagcggaag taacgtgtca
      361 tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga tcaggccgca gaggttaaac
      421 tctgcggcct ttttcgtgct tactgcgggt aaggcaccca gtcgccgccg ttcaggcgaa
      481 cgtacggttt atcttggtat tgaataacta ctgcatttga gttctcggag accggtgctg
      541 tttgtggcaa cccactggtg agttttttcc agtcaacatt gtcttcggtg aaaatcttgc
      601 catcaagaac gcgaaccacc agatcggaga tagccaggaa gctgctcggt tggtcaatga
      661 caatcggtgc cccctgatgt ggtgccttca tgccgaagaa tttcacccca acggggacgt
      721 ctgtgataga cgggctaggg atatcacgta ggccagatac ctgcattctg tcgcccttca
      781 gcgcgccgcc gtgttccggc accacgacca ccatcacttt acgacccgat ttctccagtt
      841 cagtaaagaa ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg taatccgctg
      901 ttttgctgac ccccggataa tggttgccgt catgcagtgg aagcgtgttg tagaacgtgg
      961 cactacggct gtttttatct ttttcggtaa cgtccagcca gcggttaagc acggcggtat
     1021 cgtcataaac cggcgaacca tcaaagccca gcaaaataac cggcagattt gtttgatcca
     1081 tcaattcagt ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg ccgaactggc
     1141 cgttatgtcc catcatcagg tgctgggtaa agcccagttt cgaaaggtta tcaaacagat
     1201 agcagtcgtt atttgccggt tgatacagat tagtgtgcga agtctgcccg cagctggcgc
     1261 gcagtaaacg gatcgccgcc gggccactgt aggaggtggc ggagttaaag ttcttgaaat
     1321 caatatcgaa atgcgaccac agtggatgcg acatcaaccc ggcggcttct atatccgacc
     1381 aggaaagcga acagatgtta atcaccagta gttcaaatgg ctgagcatca gcgggcagcg
     1441 aagacgggaa ggtcgattta cgtttcgcct ccgcgttata gaaattattc agccaggcgt
     1501 taaggttcgc cgttgttggc ggtgcagttt gtgcgggcat atcacccact accggtgcgc
     1561 cacccgtcgc cgcaacggtt gccgctgcgt taccacccgt cgttgttaca gtggtcgtcg
     1621 gttgtccggc tggccacaag gagaaacttg gtcccgccag ggtaagtacg ttcagccata
     1681 gcagtatggc aaccacaaaa acggtaatgc gaatccattg tgacaggaat aaccaggcca
     1741 ctaataaaac aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg acaaggtcga
     1801 ttaaataatc ggtactgaac cccgccacct gcgaaccctg gctcattatg ctttccgggc
     1861 caggcaacca gatgtcatgc cagaacaaag caaagccgat cggcagggca atccagtggc
     1921 gcaagcgatg caggctgtaa cgcggaatgg gcatcagcag aaacgcggca aacaccaaat
     1981 tgaggagcgg gtggaagtta agatatcccg cccacaacag gccaaactta accagaaaat
     2041 agaagttcca gccggaaagg ccgctccagt attgccagag ggaagaaggc atggcggtat
     2101 tttgcgtaaa ttgagtcatt ttttggttgc cctggctttt tccgtgcggc gtaacgtccc
     2161 ggccggttta acataacgag gtttagcaaa gaacaaacgt aaggtgtcgc gaatgcgtcg
     2221 caaagagtgc cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa cgataataat
     2281 ttcaatgata tcgctgatgg tcatcatgat gagcgctcca cagcatcatc taacagtcgc
     2341 atgggttctg gtattcgtcg ccagtggcga ccatcgtgct cggcattgat gaccggttta
     2401 gaactttgcg ttaaaggcag cggcattccc cattgttctg gggcaagcag gcgcatctgc
     2461 accagctcgg cactgatttg atcatcttca aaccagacca tacggtttga gaaaatgtcg
     2521 ccagtaggca atgggaaaat atgattcaac gcggtatcca gatcgttaat ccgacagaat
     2581 gagagaaaca gcaccagccg attaccgcca atggtcatga tatcgccggt acggttaggg
     2641 cgacatagcg tcagggcttg ctcaacgcgg atacccggta ccggacgtag ggcaaccaga
     2701 acgcctttac cgtgggcagg caagagaggg ttattcatca tgttgttgac ggcattacag
     2761 aacacatccc acttctggaa accacgcagt ttgagcggct gggtcattga cagcaaggta
     2821 gtgatatctt ccggtacata gcgactaaac ttctgccctt gcacgctttc gatcatcgtc
     2881 agacaacggg agagtggcgc attccacgga ataaccatat ttgcaccgca ggccaataac
     2941 aaacgttcat cggtggcgcg caggctagcg gtattttccc gcacgaggat tttcatcgca
     3001 ctaccgcgct ggcgacgcag ggtatgaatg ctgcgggcca gtggctcgat ttgcgcattt
     3061 tgctgtaaag aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt gaacaggact
     3121 tcgttattgt tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc cagtacagca
     3181 acattactca gaatgcgttt ttcgtcgctg cgtggttgga tctccgcctc ttcgctttga
     3241 actaatgtcc agataccatt ttgttgctga acgctaagct gctgacgggc gctgacccct
     3301 ttttcgttgc accagaaggc aatatccagc aaatgttggt caccctgaaa acgcaaactg
     3361 gcaagaccaa aaagtgaacg gtattcctca agcaacaatg aaaattgttt atcgttatta
     3421 tttccgggat taattaccaa aagcgaacaa tggtttaacc tgctccattt attcatttta
     3481 tccaaccatg agcgaagccg ctcggcagga atattttgcc atgcgttatt tgcgcaaaca
     3541 agaataaaaa ggtaattatg gggatcaata gaacactgca aatcacgggc caaatagtat
     3601 agaccttttt catgatttag cattgaaaat aattttattt tttccggccc ttgagaatca
     3661 tctaattgaa agattttcgc cggatcgctg tccatgctaa tgaccgcgac gtgtgcggtt
     3721 tcagcctggg atgcaattgt ttgattcgcc agactgatag catcttcatg gcgatcgacg
     3781 ttaaaccacc agacgccgcc tgctggcata tgtcgcagct catcccataa tgatgagata
     3841 ccgatagaga atacagggtc cacaatgtcc ctcatgtcac cattttgtct gattatcagt
     3901 ttactagcga aagctaagaa ataaacctac cattgaaatt aaaacttatc agatttagca
     3961 tgtgaatcaa tttagaaggt cacaagacgt gtgattttag tcttttcgtt tttaactttt
     4021 tctgttgcgt aacggaaagt caaaaagtga gcaaattccc gtctcgccgt aaacaataaa
     4081 ccggcgaaat gctcacggtt agaattgtct catgaatggt acggttattt catagggatc
     4141 aagcaaaatg aataacaatg aaccagatac tctgcctgat cccgcgatag gctatatctt
     4201 ccagaatgat attgtggcgt taaagcaggc attttcactg cctgatattg attatgccga
     4261 tatttcccaa cgcgaacagt tggccgcggc attaaaacgc tggccgttgc tggcagagtt
     4321 tgcgcaacaa aagtagggga ttggtgaatg gccgtactgg gattgcaggg ggtgcgggga
     4381 ggcgtgggga caacaaccat caccgccgca ttagcctggt cattacaaat gttgggagaa
     4441 aatgtcctgg tggtcgatgc ctgcccggac aacttgttgc gcctgtcatt taatgttgat
     4501 tttacccacc gtcagggctg ggccagagcg atgctggatg gccaggactg gcgtgacgct
     4561 gggttgcgct acacctcgca gctcgatttg ctgccttttg gtcagttatc cattgaagaa
     4621 caagaaaatc cacagcactg gcaaacccgg ctgagcgata tttgctccgg cttacagcaa
     4681 ctaaaagcca gcgggcgtta ccagtggatt ttaatcgact taccgcgtga tgcctcgcag
     4741 ataacccacc agctgctgag tttgtgcgag cactcgctgg caatcgtcaa tgtggatgcc
     4801 aactgccata tccgactgca tcagcaagcg ctgccggatg gcgcacatat tttgattaat
     4861 gacttccgta ttggcagtca ggttcaggac gatatttacc agctttggtt gcaaagccag
     4921 cgccgattac tgccgatgct cattcatcgt gatgaagcga tggctgaatg cctggcggct
     4981 aagcaaccag taggtgaata tcgcagtgat gcgctggcgg ctgaagagat actgacgctg
     5041 gcgaactggt gcctgttgaa ctactccggg ctgaaaacgc cagtcgggag tgcatcatga
     5101 gtatcctgac ccggtggttg cttatcccgc cggtcaacgc gcggcttatc gggcgttatc
     5161 gcgattatcg tcgtcacggt gcgtcggctt tcagcgcgac gctcggctgt ttctggatga
     5221 tcctggcctg gatttttatt ccgctggagc acccgcgctg gcagcgtatt cgcgcagaac
     5281 ataaaaacct gtatacgcat atcaacgcct cgcgtccgcg tccgctggac ccggtccgtt
     5341 atctcattca aacatgctgg ttattgatcg gtgcatcgcg caaagaaacg ccgaaaccgc
     5401 gcagcgggca ttttcaggtc tgcaaaatat tcgtggacgt taccatcaat ggatgaacga
     5461 gctgcctgag cgcgttagcc ataaaacaca gcatctggat gaaaaaaaag agctcggtca
     5521 tttgagtgcc ggggcgcggc ggttgatcct cggtatcatc gtcaccttct cgctgattct
     5581 ggcgttaatc tgcgttactc agccgtttaa cccgctggcg cagtttatct tcctgatgct
     5641 gctgtggggg gtagcgctga tcgtacggcg gatgccgggg cgcttctcgg cgctaatgtt
     5701 gattgtgctg tcgctgaccg tttcttgccg ttatatctgg tggcgttaca cctctacgct
     5761 gaactgggac gatccggtca gcctggtgtg cgggcttatt ctgctcttcg ctgaaacgta
     5821 cgcgtggatt gtgctggtgc tcggctactt ccaggtagta tggccgctga atcgtcagcc
     5881 ggtgccattg ccgaaggata tgtcgctgtg gccgtcagtg gatatctttg tcccgactta
     5941 caacgaagat ctcaatgtgg tgaaaaatac catttacgcc tcgctgggta tcgactggcc
     6001 gaaagacaaa ctgaacatct ggatcctcga tgacggcggc agggaagagt ttcgccagtt
     6061 tgcgcaaaac gtgggggtga aatatatcgc ccgcaccact catgaacatg cgaaagcggg
     6121 caacatcaac aatgcgctga aatatgccaa aggcgagttc gtgtcgattt tcgactgcga
     6181 ccacgtacca acgcgatcgt tcctgcaaat gaccatgggc tggttcctga aagagaaaca
     6241 gctggcgatg atgcagactc cacaccactt cttctcgccg gacccgtttg aacgcaacct
     6301 gggacgtttt cgtaaaacgc cgaacgaagg cacgctgttc tatggtctgg tgcaggatgg
     6361 caacgatatg tgggacgcca ctttcttctg cggttcctgt gcggtgattc gtcgtaagcc
     6421 gctggatgaa attggcggca ttgctgtcga aaccgtgact gaagatgcgc atacttctct
     6481 gcgtttgcac cgtcgtggct atacctccgc gtatatgcgt attccgcagg cggcggggct
     6541 ggcgaccgaa agtctgtcgg cgcatatcgg tcagcgtatt cgctgggcgc gcgggatggt
     6601 ataaatcttc cgtctcgata acccgctcac cggtaaagga ctgaagtttg ctcagcggct
     6661 gtgctacgtc aacgccatgt tccacttctt gtcgggcatt ccacggctga tcttcctgac
     6721 tgcgccgctg gcgttcctgc tgcttcatgc ctacatcatc tatgcgccag cgttgatgat
     6781 cgccctattc gtgctgccgc atatgatcca tgccagcctg accaactcga agatccaggg
     6841 caaatatcgc cactctttct ggagtgaaat ctacgaaacg gtgctggcgt ggtatatcgc
     6901 accaccgacg ctggtggcgc tgattaaccc gcacaaaggc aaatttaacg tcaccgccaa
     6961 aggtggactg gtggaagaag agtacgtcga ctgggtgatc tcgcggccct acatcttcct
     7021 tgttctactc aacctggtgg gcgttgcggt aggcatctgg cgctacttct atggcccgcc
     7081 aaccgagatg ctcaccgtgg tcgtcagtat ggtgtgggtg ttctacaacc tgattgttct
     7141 tggcggcgca gttgcggtat cggtagaaag caaacaggta cgccgatcga accgcgtgga
     7201 gatgacgatg cccgcggcaa ttgcccgcga agatggtcac ctcttctcgt gtaccgttca
     7261 ggatttctcc gacggtggtt tggggatcaa gatcaacggt caggcgcaga ttctggaagg
     7321 gcagaaagtg aatctgttgc ttaaacgcgg tcagcaggaa tacgtcttcc cgacccaggt
     7381 ggcgcgcgtg atgggtaatg aagttgggct gaaattaatg ccgctcacca cccagcaaca
     7441 tatcgatttt gtgcagtgta cgtttgcccg tgcggataca tgggcgctct ggcaggacag
     7501 ctacccggaa gataagccgc tggaaagtct gctggatatt ctgaagctcg gcttccgtgg
     7561 ctaccgccat ctggcggagt ttgcgccttc ttcggtgaag ggcatattcc gtgtgctgac
     7621 ttctctggtt tcctgggttg tatcgtttat tccgcgccgc ccggagcgga gcgaaacggc
     7681 acaaccatcg gatcaggctt tggctcaaca atgatgataa cgcgatgaaa agaaaactat
     7741 tctggatttg tgcagtggct atggggatga gtgcgttccc ctctttcatg acgcaggcga
     7801 cgccagcaac gcaaccactg atcaatgctg agccagctgt agccgcccag acggaacaaa
     7861 atccgcaggt ggggcaagtg atgtcaggcg agcagggagc tgatgccccc atcgtggcgc
     7921 agaacggtcc ttcgcgtgat gtgaagctga cctttgcgca aatcgcgccg ccgccgggca
     7981 gcatggtgct acgtggtatt aacccgaacg gcagcattga gtttggtatg cgcagcgatg
     8041 aagtggtgac gaaggcgatg ctcaacttcg aatacacgcc atcgccatcg ttactgcctg
     8101 tccagtcgca gttaaaggtt tatctcaatg atgagctgat gggcgtgctg ccagtgacca
     8161 aagaacagtt gggtaaaaaa acgctggcgc aaatgcccat taatccactg tttattaccg
     8221 acttcaaccg tgtgcggctg gagtttgtcg gccattatca ggacgtgtgc gaaaacccgg
     8281 ccagcaccac gctttggctg gatgttgggc ggagcagtgg actggatctg acctatcaga
     8341 ccctgaatgt gaagaatgac ctgtcacact tcccggtgcc attctttgac ccgcgcgata
     8401 accgtactaa caccttgccg atggtctttg cgggtgcgcc ggatgttggg ctgcaacaag
     8461 catctgccat tgtcgcctcg tggtttggtt cgcgttctgg ctggcgtggg cagaacttcc
     8521 cggtgctcta taaccaattg ccggatcgca atgccattgt ctttgcaacc aacgacaaac
     8581 ggccggactt cctgcgcgat catccggcgg taaaagcccc ggtgattgag atgattaacc
     8641 atccgcagaa tccttacgtc aaactgctgg tggtgtttgg tcgtgacgac aaagacctgt
     8701 tgcaggcagc gaaaggtatc gctcagggta acattctgtt ccgtggtgaa agcgtggtag
     8761 tgaatgaagt gaaaccgctg ctaccgcgta agccgtacga tgcgccgaac tgggtacgta
     8821 ccgatcgtcc ggtcacattt ggcgaactga aaacctatga agaacagtta caatccagcg
     8881 gccttgagcc agcagcgatt aacgtttcgc taaacctgcc gccggatctc tacctgatgc
     8941 gcagtaccgg cattgatatg gatattaatt accgctacac catgccgccg gtgaaagaca
     9001 gttcgcggat ggatatcagc ctgaataacc agttcctgca atccttcaac ctgagcagca
     9061 aacaggaggc gaaccgtctg ctgctgcgta ttccggtatt acaaggtttg ctggatggca
     9121 aaacagatgt ctctattccg gcgctgaaac tgggcgcgac caaccagctg cgcttcgact
     9181 ttgagtatat gaacccgatg ccaggcggtt cggtagataa ctgtattacc ttccagccgg
     9241 tgcagaatca tgtggtgatt ggtgacgatt ccaccatcga cttctcgaag tattaccact
     9301 tcatcccgat gccggatcta cgcgcctttg ctaacgcggg cttcccattc agccggatgg
     9361 cggatctgtc gcaaaccatc accgtgatgc cgaaagcgcc taacgaagca cagatggaaa
     9421 cgttgctgaa tactgttggt tttatcggcg cacagacggg cttcccggcg attaatctga
     9481 cggtgaccga tgatggcagc accattcagg gcaaagatgc cgacatcatg atcatcggtg
     9541 gtatcccgga caaactgaaa gacgataagc agatcgacct attggtgcag gcgaccgaaa
     9601 gctgggtgaa aacaccgatg cgccagaccc cgttccccgg cattgtgccg gacgagagcg
     9661 atcgcgcggc agaaacccag tcaacgctga cctcttccgg tgcgatggcg gcggtgattg
     9721 gcttccagtc gccgtataac gaccagcgca gcgtgattgc gctgttggca gatagcccac
     9781 gcggttatga aatgcttaac gatgcggtga acgatagcgg caaacgcgcc accatgttcg
     9841 gttcggtcgc ggtgatccgc gagtccggta tcaacagcct acgtgttggc gacgtttatt
     9901 acgtaggtca tctgccgtgg ttcgagcgct tgtggtatgc gctggcaaac catccgattc
     9961 tgctggcggt gctggcgact atcagtgtga tattgctggc atgggtactg tggcgtctgc
    10021 tgcgaattat tagtcgtcgt cgtcttaacc cggataacga gtaattgaag atgaatgtgt
    10081 tgcgtagtgg aatcgtgacg atgctgttgt tggctgcctt tagtgttcag gcagcctgta
    10141 cctggcctgc ctgggagcag tttaaaaagg attacatcag tcaggaaggg cgcgtcatcg
    10201 accccagcga cgcgcgcaaa atcaccacct ccgaagggca aagttacggt atgttctttg
    10261 ccctggcggc taacgaccgt gcagctttcg ataatattct cgactggacg cagaacaatc
    10321 tcgctcaggg ttctttaaaa gaacgtttgc ccgcctggct gtggggcaag aaagagaaca
    10381 gtaagtggga agtgctggac agcaattcgg cctccgatgg tgatgtctgg atggcctggt
    10441 cgttgctgga ggcggggcgt ttgtggaaag agcagcgtta taccgacatc ggcagcgcgt
    10501 tgctaaaacg tatcgcgcgg gaggaagtgg tgacggtgcc tgggctgggt tccatgttgt
    10561 taccgggcaa agtgggtttt gctgaggata acagctggcg ttttaacccc agctacatgc
    10621 cgccgacgct ggcgcagtat ttcacccgct ttggcgcgcc gtggaccacg ctgcgcgaaa
    10681 ccaatcaaca tttattgctg gaaaccgccc cgaaaggctt ttcgccagac tgggtgcgct
    10741 atgagaaaga caaaggctgg cagctaaaag ccgaaaaaac attgatcagc agctacgacg
    10801 ctatccgcgt ttacatgtgg gtaggcatga tgcctgacag cgatccgcaa aaagcgcgga
    10861 tgctcaaccg gtttaaaccg atggcgacat tcactgagaa aaacggttat ccgccggaaa
    10921 aagtggatgt ggctacgggg aaagcgcagg gtaaaggacc ggtcggtttt tctgccgcca
    10981 tgctgccttt tttacaaaac cgcgatgcgc aggcagttca gcgccagcgc gtggccgata
    11041 actttcccgg cagcgatacc tattacaact atgtgctgac cctgtttgga caaggctggg
    11101 atcaacaccg tttccgcttc tcgacaaaag gtgagttatt acctgactgg ggccaggaat
    11161 gcgcaaattc acactaaaca tattcacgct ttccctcggt ctggccgtca tgccgatggt
    11221 cgaggcagca ccaaccatca gcagaccctg cggatggaca gcggcaacac taatgccgtg
    11281 cgcgggctgg cgaatattta ccgccagcag tcgccagaaa aagctgaagc gtttatcgcc
    11341 tcgctctctg ccagccagcg gcgcagcatt gatgatatcg aacgcagcct gcaaaacgac
    11401 cgtctggcac agcaggcaga ggtactggaa aaccagggca aatgggcgca ggcggcagca
    11461 cttcagcggc aacgactggc gctgggcccc ggcagcgtat ggattactta ccgactggca
    11521 ggaagaagct gcgcggcaat ccaacgatgg cggcagcagt cagggcttcg gctacacggc
    11581 gcgggcatta cttgaacgac gtgttacttc caactggttt gttggcacgg caattgatat
    11641 ccagcaggtg aaagattacg cacccagcca tttcctgctc tacgtacgtt attccgccgc
    11701 cggatggcag ggtgacatgg atttaccgcc gcagccgctg ataccttacg ccgactggta
    11761 agttttcaga tagcgcctct cttaatgccg ctgcgatcgg gtatactcgg gcggcaatct
    11821 gggatttccg gggggagaca atttgcgcgt aagtcgctcg ttaacaatca agcagatggc
    11881 aatggtggca gccgttgtcc tggtgttcgt ttttattttt tgcaccgttt tgctgttcca
    11941 tctggtccag cagaatcgct ataacacggc tacgcaactg gaaagcattg ctcgctctgt
    12001 ccgcgaaccc ttatcttcag ctattttgaa aggcgatatt cccgaagcgg aagctattct
    12061 tgccagcatt aaaccggcag gcgtggtcag ccgtgccgat gtagtgctgc ctaaccagtt
    12121 ccaggcgctg cgtaaaagtt ttattccaga gcgtccggtg ccggtaatgg ttactcgcct
    12181 gtttgagcta ccggttcaaa tctcgctggg cgtctactcg ctcgaacgtc cggcaaatcc
    12241 gcagccaata gcctatctgg tgctacaggc ggattccttc cgtatgtata agttcgtgat
    12301 gagcactctc tcaacgttag tgaccattta cttacttttg tcgcttatcc tgaccgtcgc
    12361 catcagctgg tgcattaacc gcctgatttt gcatccgtta cgcaatattg ctcgcgaact
    12421 taacgccatc ccagcccagg agcttgttgg tcaccaactg gcattaccgc gtctgcatca
    12481 ggacgatgaa atcggtatgt tggtgcgcag ttacaacctc aaccagcaat tgctgcagcg
    12541 ccattatgaa gaacagaacg aaaatgcgat gcgcttcccg gtgtcggatt tgccgaacaa
    12601 agccttgctg atggagatgc tggagcaggt tgtcgcgcgt aaacaaacca ccgcgctgat
    12661 gatcatcacc tgtgaaaccc tgcgtgatac tgcgggcgtg ctgaaagagg cgcaacgaga
    12721 aattctgctg ctgacgctgg tggaaaaact caaatcggta ctgtcgccac gtatgatcct
    12781 cgcgcagatt agcggttatg actttgctgt cattgccaac ggtgtacagg aaccgtggca
    12841 cgcaatcact ttgggtcagc aagtactcac tatcatgagc gagcgcctgc cgattgaacg
    12901 tattcaactc cgtccgcact gtagcattgg cgtggcgatg ttctacggcg atctcaccgc
    12961 cgaacagctt tacagtcgcg ctatttctgc ggcatttacc gctcgccata aaggcaagaa
    13021 tcagattcag ttctttgatc cgcagcagat ggaagcagcc cagaagcggt tgacggaaga
    13081 gagcgatatc cttaatgcac tggaaaatca tcagtttgca atttggttac agccacaggt
    13141 cgagatgacc agcggtaaac tggtcagtgc ggaagtgtta ctgcgtatcc agcaaccgga
    13201 tggcagttgg gacctgccgg atggcttaat cgatcgcatt gagtgctgtg ggctgatggt
    13261 taccgtcggt cactgggtgc tggaagagtc ctgtcgattg cttgcagcct ggcaagagcg
    13321 cggcattatg ctgcccttgt cggtaaacct ctctgcgctg caactgatgc acccgaatat
    13381 ggtggcggat atgctggaac tgttaacccg ctatcgcatt cagccgggaa cactgattct
    13441 ggaagtgaca gaaagccgac gtattgacga ccctcatgct gcggtggcaa tcctccgtcc
    13501 gctgcgcaat gccggagttc gggtggcgct ggatgatttc ggcatgggct acgcagggct
    13561 gcgtcagctg cagcatatga aatcgttgcc aatcgacgta ctgaaaatcg acaaaatgtt
    13621 tgttgaaggc ttgccggaag atagcagcat gattgctgca attatcatgc tggcgcagag
    13681 cctgaactta caaatgattg ccgaaggcgt ggagactgaa gcacaacgag actggctggc
    13741 aaaagcgggc gttggtattg cccagggctt cctttttgct cgcccactcc ctattgaaat
    13801 cttcgaagag agttacctgg aagaaaagta gctaccccaa actgattaca aaactttaaa
    13861 aagtgctggt ttgtgcgagc cagctcaaac tttttaacct ttttgtttca attatgatcc
    13921 aggtacattt ctgtgatgtt gtctgggtgt tattttaagg ccgcaggtac ccccccccca
    13981 taaccttaca agacctgtgg ttttactaaa ggacacccta tgaaaacctc tctgtttaaa
    14041 agcctttact ttcaggtcct gacagcgata gccattggta ttctccttgg ccatttctat
    14101 cctgaaatag gcgagcaaat gaaaccgctt ggcgacggct tcgttaagct cattaagatg
    14161 atcatcgctc ctgtcatctt ttgtaccgtc gtaacgggca ttgcgggcat ggaaagcatg
    14221 aaggcggtcg gtcgtaccgg cgcagtcgca ctgctttact ttgaaattgt cagtaccatc
    14281 gcgctgatta ttggtcttat catcgttaac gtcgtgcagc ctggtgccgg aatgaacgtc
    14341 gatccggcaa cgcttgatgc gaaagcggta gcggtttacg ccgatcaggc gaaagaccag
    14401 ggcattgtcg ccttcattat ggatgtcatc ccggcgagcg tcattggcgc atttgccagc
    14461 ggtaacattc tgcaggtgct gctgtttgcc gtactgtttg gttttgcgct ccaccgtctg
    14521 ggcagcaaag gccaactgat ttttaacgtg atcgaaagtt tctcgcaggt catcttcggc
    14581 atcatcaata tgatcatgcg tctggcacct attggtgcgc tcggggcaat ggcgtttacc
    14641 atcggtaaat acggcgtcgg cacactggtg caactggggc agctgattat ctgtttctac
    14701 attacctgta tcctgtttgt ggtgctggta ttgggttcaa tcgctaaagc gactggtttc
    14761 agtatcttca aatttatccg ctacatccgt gaagaactgc tgattgtact ggggacttca
    14821 tcttccgagt cggcgctgcc gcgtatgctc gacaagatgg agaaactcgg ctgccgtaaa
    14881 tcggtggtgg ggctggtcat cccgacaggc tactcgttta accttgatgg cacatcgata
    14941 tacctgacaa tggcggcggt gtttatcgcc caggccacta acagtcagat ggatatcgtc
    15001 caccaaatca cgctgttaat cgtgttgctg ctttcttcta aaggggcggc aggggtaacg
    15061 ggtagtggct ttatcgtgct ggcggcgacg ctctctgcgg tgggccattt gccggtagcg
    15121 ggtctggcgc tgatcctcgg tatcgaccgc tttatgtcag aagctcgtgc gctaactaac
    15181 ctggtcggta acggcgtagc gaccattgtc gttgctaagt gggtgaaaga actggaccac
    15241 aaaaaactgg acgatgtgct gaataatcgt gcgccggatg gcaaaacgca cgaattatcc
    15301 tcttaatctc accacttatg cccgcactcc cttccccgag tgcgggtgtt tgcgcataat
    15361 tgacccctgt gcctgaactt accacctggg tgagttcagg tttatttcac ttcttccgtg
    15421 acattttcat gttcttgcgg tctaacacga agtgttttta acgtcattta gactgaccga
    15481 caacgggcag tttttttatt accaggatag ttgatcaggg gttcacatgc agggcataaa
    15541 aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg tgcaggcaga
    15601 tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt tacagtggca
    15661 agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc tggttaatac
    15721 cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc ctcgtattgc
    15781 gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc agcaggggat
    15841 cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc tgtttaatct
    15901 gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc tggcaaatgc
    15961 cactggcaaa ttgaccatca caccagaaac catcaaccac gcgctgcaaa gtcaggacat
    16021 ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc tgaaagggtc
    16081 aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag cggaaaaaat
    16141 taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg tggtgggaaa
    16201 cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg tttggcgaac tgaaaggcaa
    16261 acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag cggtgagtat
    16321 tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc cgtggcagcc
    16381 gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc gcgaggcgct
    16441 gttctggcat gttcagcaag cgttaagcgc cagtgacagc aaagacatcg gtcttggatt
    16501 tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat caccaaacga
    16561 caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc gcgataaagg
    16621 tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc tgcagaaact
    16681 gtttgccgcc tatgcacggg ctgataccga tattctgatg ggccagcgga tgcgttcgtt
    16741 gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc aggatttcct
    16801 taacagcctg actgtggaga tgttaaatca ggatctgcgt cagcagttgt cgaatgatat
    16861 ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag cgttgcaggc
    16921 ggcctgggat caaatcatgg ccccatctac cgccgctgcg accacctctg tcgccacgga
    16981 tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga tggtgatgag
    17041 ttagcacaac catcaggtat ttatggatga gctggataga ggggtaacac tttatccagc
    17101 cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg cgccgcgata
    17161 ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg cactgccgcc
    17221 cgtcaaacgt accgccagat aaccggcgct gaaagagtcg ccagctgcgg tggtatcgat
    17281 cactttttct ttcggcagtt tcaccgccgg aacatcaact aacgcttcac cagcaatgga
    17341 caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg cgttatgggt
    17401 gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcgt cgtccagcgt
    17461 caggaaggcg atatccgtgc attccagcat ttgctggtac acctgctgtg tctcttcttt
    17521 gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc ctttggcgcg
    17581 gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga tcgccaggct
    17641 aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg ccgcagactg
    17701 ctcgctcgcc agccagaatt tggcggcggc ttcgttacgc cagtagtaga acgtacgttc
    17761 gccggtgctg tcggtttcaa tgtagtaaag gcccggaaga cggttttcca tccgttgggt
    17821 cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atctgctggc taaaactgtc
    17881 cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct gacgggcgat
    17941 atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg cgcctttctc
    18001 ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag tcgtgaacct
    18061 gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa ccatattaaa
    18121 acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg ttgtggagtg
    18181 aggaaattta ccccggccag gcggtaaagt tctgccctta cgcgccgata atctttgtcg
    18241 agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca ggttatccag
    18301 cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt ttggttgcag
    18361 tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcgggcggtt aatggccgtg
    18421 gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc gccggatcgc
    18481 tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca gattgatttg
    18541 ctggcgcaaa aagccgactt ctttgtagag cacggcctgc tggcatcggt caatattgat
    18601 ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccagat tgagcgtctt
    18661 ccctggctgc gtttcgaact ggtggagcat atccgtctgc cgaaagattc aacctttgcc
    18721 tcgatgtgtg aatttggccc gctgtggctg gatgattttg gtaccgggat ggcaaatttc
    18781 tctgcactaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagaact gtttgtgatg
    18841 ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc ttttacatct aatgaatcgc
    18901 tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg aagagtggcg tgatgttcag
    18961 aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc gatggaaacg
    19021 ctgaatacgg cggttctggc gctataagct gcctcatttt ccgccgggct ggactatctt
    19081 taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg caaaataacc
    19141 gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt gattgcaact
    19201 tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga gttgaatcgt
    19261 ccgttcgcta tccgtggcaa tctgggcgtg gtgtgggagc ggcaaaaaca agaaactggc
    19321 tggcgcagct gggtgccgtg gcctcatgta catgcagaag acatcattct tggtaatcca
    19381 ccggatattc ccgaagtcac gatggtgcat ttgccgcgag tagaggcaac gctggcaccg
    19441 ctggcgctgc tgaccaaaac ggtctggctg ccgtggatca agctcgaaaa gcccgacgcg
    19501 cgcctgattc gcctctctga aaagaacaat aactggacgt ttaatcttgc caacgatgac
    19561 aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa tattcttttc
    19621 gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga aatttttgtt
    19681 gatccgttag gcaagccgct gccattcagc gaagttactg gatcgaaagg taaagcggat
    19741 aaagaaaagg tgggcgatta catttttggc ctgaaggcgc agggacgtta taacggtgaa
    19801 ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga agggacgccg
    19861 tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga tggcgtcgtg
    19921 aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg cgattcactg
    19981 ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt tgaaacggat
    20041 ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta tcgcggtttt
    20101 aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac cggaaagcca
    20161 cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga cctgggaccg
    20221 ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga acagaagaag
    20281 ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt cgaaaccgat
    20341 aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga gcatggcagt
    20401 agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga cctgcgcctg
    20461 caaccgctga aatttggcat ggcgggcggc agcattgcgg cgaatattca tctggaaggc
    20521 gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa actgaaagaa
    20581 ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg tgacgcggaa
    20641 ctacgcggta gcggtaactc ggtggcggcg cttttaggca acagtaacgg caacctgaaa
    20701 ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg gctgaatgtc
    20761 ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa ctgcgcggcg
    20821 gcgaatctga atattgccaa cggcgtggcg cgcccgcaga tttttgcttt cgatactgag
    20881 aacgcgttga ttaatgttac cggcacggca agttttgctt cggaacagct ggatttgact
    20941 attgatccgg agagtaaagg gattcggatt atcacactgc gttcgccgct gtatgtgcgt
    21001 gggacgttta aaaatccgca ggctggggtg aaagccggac cgctgattgc ccgtggtgct
    21061 gttgctgcgg cactggcaac gctggtaaca ccggcggcgg cgttactggc actgatctca
    21121 ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa gaagtgatga
    21181 taattgccgg atgtctgaca tccggcaatt aaacagattt agatttttgg caaagtgtgc
    21241 tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct tgtgaattca
    21301 atatattgta tggaaattca gtcctgataa gcgtagcgca tcaggctttt cacacttatt
    21361 acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc aagccagcca
    21421 tcgccgccag atataacccc accgcaccta acccgtagtt agcctgcaac caggctgcga
    21481 tatacggcgc aacggaagcc ccgagaatcg acgatacgtt gtaagagaac gatgctccgg
    21541 tgtaacgcac ttctgtcgga aacagctctg gtaacagcgc acccatcggc ccgaaggtca
    21601 gccccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg ttgccagaac
    21661 cgagcagcgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg atgatcacca
    21721 tgcttttacg gcgaccaaag gcatcagcca gtaatccggc gactggcacc atcacgccaa
    21781 aaccaataac tgccatcatc agcatccaca acacttcgtt acgcggcagg ccaagcccaa
    21841 ctggcgcggc ggcggtactg aaggtcatag agtagaccgt catgatgtaa aacagcgtat
    21901 aggttgccag cataatgaac gtacccagta cggttacgcg aacatgtttg gtcagcagcg
    21961 tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac accggcgact
    22021 catgcagcga cacgcgaaca tacaggccga taatgaccag caccgccgag aagataaatg
    22081 gcacacgcca gccccagctc ataaactgct catcggtcag tagccaggaa agcagcaaga
    22141 aagtgccgtt ggcaaagaag aagccaatcg gtgcgcctag ctgtgggaag gagccataca
    22201 gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg ccccattcac
    22261 cgcctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagtggg gcgaaaatac
    22321 caatcgtggc atagcccggc agcagaccaa tcaccacggt cgaaatcccc atcgttagca
    22381 acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca aaaacggcag
    22441 agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt agcgttgctg
    22501 ccgttggatc gccctgcggg aagaagatat gcggaaacac aataacggcc gcagtggcgt
    22561 aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca aggactttat
    22621 tacgcgagtt gatcggcgtg tattcttgtt cgtggtcgag tgttgtggct gttgcttgca
    22681 taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa aagtgatatt
    22741 tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga taatgtttag
    22801 gccagtgatt tttcttctat gaaatgcttc acataatttt ataaatagtg gataaaactg
    22861 gtttcaggtt aaataaaagt accaactgga tttatatgct ggggttatga gttggagtga
    22921 aattgtttgc tcccaaatag atggctgaag aggctctggg gaagagtttc aagaggttat
    22981 gtgcgcatta tagccggatg cgatgctggc gcatcttatc cggcctacgg ggagccaggc
    23041 cgaataaaat cgtatgccgg ataaggcgtt tacgccgcat ccggcctatt ttaaggctgc
    23101 gttttccctg gcattcgcgg gtcgtcttta tattcggcgg tggcaatcca cgccgcgcaa
    23161 aacagcgtca aacgggcgaa gaagtagaaa aatgccatca gccccagcac ggaaccaaat
    23221 gctgcgccag agggggattt catcagcgat ggcagggtgt aggtcatcac gattttaatc
    23281 acttcaaaac caatagcagc cagaaatgtt ccgcgaatca gggctttttt gcgcggacgg
    23341 tggcgcggca accgccagaa gatccagaag aaaagcagat agttggcgaa gatggaaatc
    23401 gccaggccaa tcaatcgcca cgtcggtttc agccactcaa tgctgttcag gtgcagggcg
    23461 ctaataatca tttgctgcgc cgaaccggca accgaggtga tcgaaagcgt cacaatcagc
    23521 gcaatcaaca aaccaatcag tgaaataaaa tcacgcagat attttaccca gaacttctcc
    23581 tgatcttgcg gcgagcgttc ccagacatcg cgcgactggg cacgaatcgc ttcacgcagg
    23641 ttacccatcc agttgatgcc ggaataaagt gccaccgcca ggccgacaag ccctacagtc
    23701 gtacgctgct gaacggcggt gttgatggtg tttttcaacg tggcggctag tgtcggatcg
    23761 ctgatgtttt gcagaatttt gtcgaagata tcctgtagca acatcggatg ggaggccagc
    23821 acaaagcccc ctgcggcaaa cgacaccatc aaaatcggga tcatcgacaa aaatgagaaa
    23881 taggtgatag ccgcaccaaa ctggttaccc agccgatcgt taaagcgttc tgtcgcgcga
    23941 ataaggtgcg caataaccgg ttggcgttgg actttttcgg cagtggtggt gacggtttcc
    24001 agcgcctggc taactttgcc gcctttctcg ctattatcca actgcttaat cggctcgtgc
    24061 tccagatcct gggtgggacg tttgatctcg ttttcctgcg tcatccgctc ttttgtcctt
    24121 ctgtcgtggt cattcttaaa aagtatagtc ggtcagtcca ggtgctcttt cattacgcca
    24181 gccagccact gcataaacag gtttacacgc cgggaaagct cccgacgctg cgggtaaacc
    24241 agcgaaaggg agagcggctc ggcacggtag cctggcaaca cttcaataag ccgcccggca
    24301 cgcagggctt cgcgcacggc aatgcgcggg atctgaataa tccccagacc ggcaagaccg
    24361 gcggcgagat agttttcgct actgtttacc gtcaacatgc cgccagactt aaaccactgg
    24421 acgccattaa cgctggcaac ctcaaaacct aacggatgta cacccaggtg cggtgtataa
    24481 cgcactattg cgtgtgaagt cagatcatcg gggctttgcg gataaccaaa gcgcgtcaga
    24541 tagtgcggac tggcacagtt gaccatggtt agtttgccga ggggacgggc gataacgcca
    24601 tcttccggta acgcgccagt gcgtatcacg caatcaaaac catcatgaag aatatctacc
    24661 ggacggttat ggctactcag ttccagctca attcccggat gctgatagag aaattccgac
    24721 agacgcggca gtaacaggtt tttcgcgatt cctgacggga tgtcgatgcg taatttaccg
    24781 ctgatactgg tggcatcctg ttgaaacaga ccgtccagtt cgctgagatt actcaacaca
    24841 tcttttgccc gttgataata ggtcatgcct tctggcgtga gtttgacccg tcgcgtagtg
    24901 cgctggagaa gctgggtgcc aagttgatgt tccagtgcct gtatctggcg cgaaacactt
    24961 ccctttggca aagcaaagaa atccgccgcg cgggaaaaac tttccagctc cgcgactttg
    25021 atgaacaact gcattgcgtg aattttatcc atttctttga ccgattgttg ttcacagcga
    25081 aacagtaacg cgcatatcgc attgtttatt gttttttcta acatctaatg ccgggtttca
    25141 caatgttgtc atgacgaatg tggcgatttc tcagcaggaa aagggcggaa tagctttatg
    25201 agaaaggtga atccccttaa acagagcgta tttttcagtg attcccttta aagtgaatag
    25261 aggacgtatt ggctatttgt gcttattttt gtctcattgc catttgttat tttggcttgt
    25321 agtcgcggaa tttcattttt aattgcatgc gtactaacca ccaatataag gaaatcctca
    25381 aaattgtttt aaatagtgcc ctcgacatgt aatttcaggc gtttagctgt aactatttat
    25441 atcggtaact attttctacg cgtcatctct gtagaatggc caccgccaaa tggcgaattc
    25501 ttattttttc tcgcgggtaa tacccttcca ctacttaagt ctttttaacg tattcaggtg
    25561 gagattcagt tatgcaaata gtcatgtttg acaggcagtc aatatttatt catggaataa
    25621 aaatcagttt acagcagcgt attccaggag tgagtattca gggggtcagt caggcagacg
    25681 agttatggca aaagctggaa agttaccctg aagccttagt tatgctcgat ggcgatcagg
    25741 atggtgagtt ttgctactgg ttgctgcaaa aaaccgtggt gcagtttcct gaggttaagg
    25801 tgttaatcac ggcgacggat tgcaataaac ggtggttaca ggaagttatc cattttaatg
    25861 tgctggccat tgtgcctcgc gattcaaccg tcgagacatt tgcgttggcg gtaaatagtg
    25921 cggcgatggg gatgatgttt ctgcctggag actggcggac gacgccggaa aaggacatta
    25981 aagatctcaa atcgttaagc gcccgccagc gggagatttt aaccatgtta gcggcgggcg
    26041 aatcaaataa ggagatcggc agagcgctga atatcagtac cggaacggta aaagcgcatc
    26101 tggaatccct gtatcgccgt ctggaagtga aaaatcgcac ccaggcggca atgatgttaa
    26161 atatctcctc ctg
//
LOCUS       DAZSNM010000032        25704 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZSNM010000032 DAZSNM010000000
VERSION     DAZSNM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25704
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.032"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..954)
                     /gene="rffG"
                     /locus_tag="ACHW42_001168"
     CDS             complement(<1..954)
                     /gene="rffG"
                     /locus_tag="ACHW42_001168"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR8943071.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQE"
     gene            complement(954..2216)
                     /gene="wecC"
                     /locus_tag="ACHW42_001169"
     CDS             complement(954..2216)
                     /gene="wecC"
                     /locus_tag="ACHW42_001169"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR8943072.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(2213..3343)
                     /gene="wecB"
                     /locus_tag="ACHW42_001170"
     CDS             complement(2213..3343)
                     /gene="wecB"
                     /locus_tag="ACHW42_001170"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR8943073.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(3399..4445)
                     /gene="wzzE"
                     /locus_tag="ACHW42_001171"
     CDS             complement(3399..4445)
                     /gene="wzzE"
                     /locus_tag="ACHW42_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR8943074.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(4457..5560)
                     /gene="wecA"
                     /locus_tag="ACHW42_001172"
     CDS             complement(4457..5560)
                     /gene="wecA"
                     /locus_tag="ACHW42_001172"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR8943075.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(5800..7059)
                     /gene="rho"
                     /locus_tag="ACHW42_001173"
     CDS             complement(5800..7059)
                     /gene="rho"
                     /locus_tag="ACHW42_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR8943076.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(7144..7245)
                     /gene="rhoL"
                     /locus_tag="ACHW42_001174"
     CDS             complement(7144..7245)
                     /gene="rhoL"
                     /locus_tag="ACHW42_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR8943077.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(7386..7715)
                     /gene="trxA"
                     /locus_tag="ACHW42_001175"
     CDS             complement(7386..7715)
                     /gene="trxA"
                     /locus_tag="ACHW42_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR8943078.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            7846..9111
                     /gene="rhlB"
                     /locus_tag="ACHW42_001176"
     CDS             7846..9111
                     /gene="rhlB"
                     /locus_tag="ACHW42_001176"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR8943079.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            9245..10729
                     /gene="gppA"
                     /locus_tag="ACHW42_001177"
     CDS             9245..10729
                     /gene="gppA"
                     /locus_tag="ACHW42_001177"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR8943080.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(10776..12797)
                     /gene="rep"
                     /locus_tag="ACHW42_001178"
     CDS             complement(10776..12797)
                     /gene="rep"
                     /locus_tag="ACHW42_001178"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR8943081.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            12884..13165
                     /gene="ppiC"
                     /locus_tag="ACHW42_001179"
     CDS             12884..13165
                     /gene="ppiC"
                     /locus_tag="ACHW42_001179"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR8943082.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(13211..14686)
                     /gene="ilvC"
                     /locus_tag="ACHW42_001180"
     CDS             complement(13211..14686)
                     /gene="ilvC"
                     /locus_tag="ACHW42_001180"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR8943083.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            14836..15726
                     /gene="ilvY"
                     /locus_tag="ACHW42_001181"
     CDS             14836..15726
                     /gene="ilvY"
                     /locus_tag="ACHW42_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR8943084.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(15723..17267)
                     /gene="ilvA"
                     /locus_tag="ACHW42_001182"
     CDS             complement(15723..17267)
                     /gene="ilvA"
                     /locus_tag="ACHW42_001182"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR8943085.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(17270..19120)
                     /gene="ilvD"
                     /locus_tag="ACHW42_001183"
     CDS             complement(17270..19120)
                     /gene="ilvD"
                     /locus_tag="ACHW42_001183"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR8943086.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(19185..20114)
                     /gene="ilvE"
                     /locus_tag="ACHW42_001184"
     CDS             complement(19185..20114)
                     /gene="ilvE"
                     /locus_tag="ACHW42_001184"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR8943087.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(20134..20397)
                     /gene="ilvM"
                     /locus_tag="ACHW42_001185"
     CDS             complement(20134..20397)
                     /gene="ilvM"
                     /locus_tag="ACHW42_001185"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR8943088.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(20394..22040)
                     /gene="ilvG"
                     /locus_tag="ACHW42_001186"
     CDS             complement(20394..22040)
                     /gene="ilvG"
                     /locus_tag="ACHW42_001186"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR8943089.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(22043..22093)
                     /gene="ilvX"
                     /locus_tag="ACHW42_001187"
     CDS             complement(22043..22093)
                     /gene="ilvX"
                     /locus_tag="ACHW42_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR8943090.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(22179..22277)
                     /gene="ilvL"
                     /locus_tag="ACHW42_001188"
     CDS             complement(22179..22277)
                     /gene="ilvL"
                     /locus_tag="ACHW42_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR8943091.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            22629..24108
                     /locus_tag="ACHW42_001189"
                     /pseudo
     CDS             22629..24108
                     /locus_tag="ACHW42_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(24133..24471)
                     /gene="maoP"
                     /locus_tag="ACHW42_001190"
     CDS             complement(24133..24471)
                     /gene="maoP"
                     /locus_tag="ACHW42_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR8943092.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            24590..25429
                     /gene="hdfR"
                     /locus_tag="ACHW42_001191"
     CDS             24590..25429
                     /gene="hdfR"
                     /locus_tag="ACHW42_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR8943093.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(25525..25600)
                     /locus_tag="ACHW42_001192"
     tRNA            complement(25525..25600)
                     /locus_tag="ACHW42_001192"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25565..25567),aa:Trp,seq:cca)
     gene            complement(25609..25685)
                     /locus_tag="ACHW42_001193"
     tRNA            complement(25609..25685)
                     /locus_tag="ACHW42_001193"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25649..25651),aa:Asp,seq:gtc)
BASE COUNT         6114 a         6912 c         6570 g         6108 t
ORIGIN      
        1 ttcctgcggc agccagccaa gttcacgggc aattttcgaa gcatcaatgg cataacgcag
       61 atcatgcccc ggacggtcag cgacaaaggt gatcaggtca cgatagtacg ccacgccgtg
      121 cggcttgttc gaagccagtt cttccagcag ctcgcaaatg gtttccacaa catcgagatt
      181 cttacgctcg ttgtgaccac caatattata ggtttcaccg actttcccgg tggtcgccac
      241 gcaatacagc gcgcgggcgt gatcttccac atacagccag tcacggattt gctgcccgtt
      301 gccatatacc ggcagcggtt tacccgccag cgcgttgagg atcatcagcg ggatcagttt
      361 ttccggaaag tggtaaggac cgtagttatt cgagcagttg gtgatcagcg tcggcagacc
      421 gtaggtccgc agccaggcgc gcaccagatg gtcgctgctg gcttttgacg cggaataagg
      481 gctgctcggc gcatacggcg tggtttcggt gaagaaatca tccgtcgagt gcaggtcacc
      541 atatacttcg tcggtggaga tatgatgaaa acggaacgct gattttttat cttccgtcag
      601 cgtattccag taagcccgcg ccgcttcaag caatgtataa gtcccgacaa tgttggtttc
      661 aataaacgct gccgggccgt caatagaacg gtcaacatgg ctttcggctg ccagatgcat
      721 gacacagtct ggctgatgct cagtgaatac gcgtgccagt tctgcccgat cgcagatatc
      781 aactttctca aaggcaaagc gctcgctttg cgcgaccggt gccagcgaca tcaggtttcc
      841 ggcgtaggtc agcttatcga ccactaccac cgcatcgctc gtttcgttga tgatataacg
      901 caccagcgcc gagccaataa acccggcacc acctgttatc agaatttttc tcatcagcgc
      961 cagactcctt tggcatcgac gacatactgc tgatggacat tgtcgccatt gataactttg
     1021 aactgactat gatcgaccag catcaccagc acgtctgccg ttgccagcgc ctcgtcaagc
     1081 tgcgccagag tacaaagccc ggtcagtttt ttcggcaact ggtggatgtt aggctcaaca
     1141 accagcgttt cgccgctgtg ccactgggcg atcagttcag cgatttccat cgccgggctt
     1201 tcgcgcaggt catcaatatt cggtttaaac gccagaccaa agcaggcgat tttcagttca
     1261 ctggctcgtt tatcggtagc agcaaggcag tcggcaaccg ccgctttcac ctgatcgata
     1321 acccagaacg gtttgtgatc gttaacttcg cgcgcggtac ggataagccg cgcctgctgg
     1381 gggttctgtg ccacgataaa ccacggatca acagcaatgc agtgaccgcc cacgccaggg
     1441 ccaggctgaa gaatattaac gcgagggtga cgattcgcca ggcgaatcag ttcccagacg
     1501 ttaatcccct gatcggcaca aatcagcgac aattcattag caaaagcgat attcacatcg
     1561 cggaagctgt tttcggtgag cttacacatt tccgccgtcc gcgagttagt gacgacacac
     1621 tcaccttcga ggaaaatttt gtacaattcg ctggcgcggg ccgaacaaac cggcgtcata
     1681 ccaccaatca cgcgatcgtt tttaatcagc tcgaccataa cctggcccgg taatacacgt
     1741 tccgggcagt aagcaatgtt gacgtccgcc tgctcgccca cctgctgtgg gaaagtcaga
     1801 tccgggcgca tctctgctaa ccattctgcc atcttctcgg ttgaccccac cggcgaggtg
     1861 gattcaagga tcaccagcgc gccttttttc agcactggcg caatggagcg agcagccgat
     1921 tcaacgtagg tcatatctgg ctcatgatcg cctttaaacg gcgtaggtac agcaatcagc
     1981 caggcatccg cttcaactgg cgtcgtgctc gctcgtaaaa aaccgccttc tacggcagtt
     2041 tttactacgc tcgccaaatc aggctcgacg atatggattt cgccacgatt gatggtatca
     2101 accgcgtgtt ggttgatatc gacaccaatc acctgttttt gccgtgaggc aaacgctgct
     2161 gctgtaggca gcccgatgta acccagtccg ataacagaaa tggtcgcaaa actcatagtg
     2221 atatccgatt attttttaac gcttccagaa tgcgagagca tgcctgacca tcaccatacg
     2281 ggttatgggc gcggctcata gcttgatatt cgttttcgtc ttttaaaaga cgcgtcactt
     2341 cctcgacaat tcgctgctta tccgtgccta ccagacgcac cgtacccgcc gtcaccgctt
     2401 ccggacgctc agtggtatcg cgcatcacca gcaccggttt ccccagcgaa ggcgcttctt
     2461 cctgaatgcc gcctgagtca gtcaaaatca gccaggcgtg gttcatcagc cagacaaacg
     2521 gtaaatactc ctggggatcg atcagaatga catttttcac atgccccaga atgcgattga
     2581 ccggctctct gacgttcggg ttgagatgca ccggatagac aatctggatg tcctggtgcg
     2641 tggtggcgat gtctgccagc gcgtggcaga tttcttcaaa gccacgaccg aaactctcac
     2701 gcctatgacc ggtcaccaga atcatctttt tatcgggggc gataaacggg taatttgccg
     2761 ccagttctga acgcagcgtg tcgctgctca tcacctggtc acgcacccat aacagtgcat
     2821 caatgactgt attaccggta atgaagattc ggctatccgc aacgttttca cgcagcaagt
     2881 tttgccggga agtttcggtt ggagagaagt gatacatcgc cagatgcccg gtcaatgtac
     2941 ggttagcctc ttccggccac ggcgaataga gagcgcccgt gcgcagacca gcctcaacgt
     3001 gaccaacagg aatacgctga taaaacgccg ccaggctggt tgccagcgtc gtcgtcgtat
     3061 cgccgtgaac cagcacgacg tctggtttga actcggcaag aataggtttt agcccttcca
     3121 gaatccgaca ggttatctct gtcaggccct gtcctggctg cattatgttg agatcgtagt
     3181 caggtacaat ggaaaagagt ttcagtacct gatcaagcat ctcccgatgc tgcgcagtga
     3241 cgcaaacttt agcctcaaaa aaaggatctt ttgccaacgc atgcaccaac ggagccatct
     3301 tgatggcttc cgggcgcgta ccaaatacag tcagtacttt cacatcgatt ctcttcgaat
     3361 aagcggcgag cgcctttgcg ctcaccgcag cagtgttgct atttcgagca acggcgggtt
     3421 aatgcgacac cagccccgat cagccccccg acaatgcccc acataatcat caggaaggca
     3481 cgacgtgggc tatcgcgttt taccggttct tccggcgtac gcaaatagcg ataggtctga
     3541 aaacgcggat ccagggttgg accaacattc agggtgttta acatggctcg attctgatca
     3601 tagtcgagat caaaggccgg accgacggcc tgtaaatttt ccagtcgagc ctggagcatt
     3661 ggacgcccaa gcaggaacat ttctgaatca ggtaattcct cggcaggcac atctgtcgca
     3721 ctgcgcgaaa tattatgctg ctcagcaatt ttcagcgcct gctcaatact gttcatccgg
     3781 cggtcgtaga tggctttcgc cacctcttcc tgacgcttca cctgagcttt catctggatg
     3841 gtgcgtgccg cccatgcgcc tttcagctca tcattcagat ggctggctgc acgctggctg
     3901 gcaaaagcaa catactgacg taacaggtta ttagcgtcag gcgcggtttc ggcaataagc
     3961 ttcacgctgt cattgaccgc gcgagtaaag tctccgggga taaactggat gttgttaatc
     4021 atttcatcca gcaacgccgc atcggctttg ctgttgccca ccatccgctg tttgtaatag
     4081 tcggtttgca gccagaactc tctgcgggta tcccacgagg ccagttgcat aacaaactct
     4141 ttataggctt cgtccatgac cgatggttga tcggcagaag ccatgtttga acggacatcc
     4201 aggttacgca aaaattgctg ctgcgagtaa tatcccccca gcatattcac cgttggacga
     4261 tcggtaatcg ccgtcgagct ccactcctga cgagcaaaaa aagtatacgc cagcgcgatt
     4321 aacgcaaacg ccagccccat gccaataatc catagcttcc cagcccacaa ggtacgaaac
     4381 aacccacgaa tatccagttc attttcagcg tcttcggccg gtttcccagg cattggttgt
     4441 gtcatcacat cctcatttat ttggttaaat tggggctgcc accacgattt ctacgcagtc
     4501 tgcgttttac gcgcttaata aagcgagcaa ctttccaggc acgcttaatg caatatccat
     4561 agaggaagaa tgctagcaaa aagagcacca gcatgaccca ctccgggaca aaatgagaat
     4621 attctgccag cacgccaatg gaagcgagca gtgctgcggc aagggtaatc agcacaaacg
     4681 cctgacggga agtaaacccg gcacgcatga tcaaatggtg aatatgctga cggtcaggag
     4741 agaatgggct catgccttta cgcaggcgac ggtacataat cgccaccata tccattagcg
     4801 gaatggcgat tatccacaaa gcggtaaccg ggctgatggg atgggttttg ccctgggtcg
     4861 tttcgagcag gatccagata acggtaaaac caatcagcgt actgcccgca tcacccataa
     4921 agactttgta gcggcgaccc aggataccaa ggttaagcat gatgtatggc aggatggcgg
     4981 cgatcatcgc aaagcaccag attgcgaggc tggtttgccc gtcgaaccac aaaatcatac
     5041 cgattgctgc aaacgagacg caggacaacc cgcccagcaa gccatcaatg ccatcaacca
     5101 tgttgaacgc attaatggcc gcccagacgg caaatagcgt caggaagtaa ccaaacggtc
     5161 cgagcaccat ctcccaggag ccaaagatat aacccaggct actgagataa agtttgccga
     5221 acaccatcat aacaatgcca acagcggcct gtatggtggc acggattttt acgctgatat
     5281 caaaacgatc atccagcgcg ccaatgaaaa caagcacacc ggcacaagcg agatagagag
     5341 atgcatgcgg aatatagtaa tcgacaattc cgaacgtgaa gcaaatccct gcgtaaaccg
     5401 aaatcccccc aacgagaggt atcaatccct ggtgacgttt gcggaagttt ggtttatcca
     5461 ctaaaccgac tttttttgcc accttacggg caaaaaacag aaacagtgtc gtgaataaaa
     5521 aaatactgat gagatcagta ctcactgtca gtaaattcac aatgcatgct ctcagagaaa
     5581 attattagca gaagtataac cacgaagacc tttattcaga agggaaagtc cgttccgaac
     5641 ccaccagccc tgtgcaaaaa tcaaccattt gatatgcaaa ttgcataaca tgccagcaaa
     5701 ttccaggaat tttcctaaca tcgccagcgc gacattaaga ttacagatat aaaagcaaaa
     5761 cgccacgtaa acacgtggcg tttttggcat aagacaaatt tatgagcgtt tcatcatttc
     5821 gaagaaatcg tcattggtct tggtcatggc cagtttatta atgaggaatt ccattgcatc
     5881 gatttcgccc atcgggtgaa tgattttgcg caggatccac attttctgca gttcttcctg
     5941 agtcgtgagc agctcttctt tacgggtacc ggaacggttg tagtcgatag ccgggaagac
     6001 gcgtttttca gcgatcttac gagagaggtg cagttccatg ttgcctgtac ctttaaactc
     6061 ttcgtaaata acttcgtcca ttttagaacc ggtatcgata agcgccgtcg cgataatggt
     6121 caggctgccg ccctcttcca cgttacgcgc cgcaccaaag aagcgtttcg gacgatgaag
     6181 ggcattggcg tccacaccac cggtcaacac tttacctgac gccggaacaa cagtgttgta
     6241 agcgcgcgcc agacgagtga tggagtcgag cagaatgata acgtctttct tgtgctcaac
     6301 caggcgtttg gccttctcga tcaccatttc cgcaacctga acgtggcgag atgcgggttc
     6361 gtcaaaggta gaagcaacaa cttcaccttt taccagacgc tgcatctcgg ttacttcttc
     6421 cggacgttcg tcgatcagca gaaccatcag cacacaatcc gggtggttgt aagcaatgct
     6481 ctgagcaatg ttctgcagca gcatagtttt accggctttc ggcggtgcca caatcagacc
     6541 acgctgacca cgaccgatag gtgatgccag atccagtacg cgagcagtta aatcttcagt
     6601 agaaccgtta ccacgttcca tacgcagacg agagtttgcg tgcagcgggg ttaagttctc
     6661 aaagaggatt ttgttgcggg cgttttcagg tttgtcgaag ttaacttcgt taactttcag
     6721 cagcgcaaaa tagcgttcac cttctttcgg cgggcgaatc ttaccagaga tggtatcacc
     6781 agtgcggagg ttgaaacggc ggatttggct aggggaaacg tagatgtcat caggaccggc
     6841 gaggtaggag ctgtctgcgg aacggaggaa accaaatcca tcctgcaata tctccagtac
     6901 gccatcacca aagatatctt cgccactctt tgcgtgctgc ttcaggatgg caaaaataat
     6961 gtcctgctta cgcatgcgag ccaggttttc cagccccata ttttcgccga gagtgatcag
     7021 ctcagaaacc ggcgtattct ttaattcggt aagattcata gtggtgtgag ttcttaaact
     7081 tggggtaaat ctcgaactta atgttgtgaa tggtatggca ggatcatcca tgcctgttta
     7141 cggtcatcga ctcatgtctt ttcgctgcct ggtcacagga gagtacgcag aactgaaacg
     7201 acaagacgga ttgagtgacg agccagaaat ctgttcactt cgcatttaag ataaaacggg
     7261 aagcgttgga aattacaaga ttcaaactta ataaggtatg tttaatacga agtcaacact
     7321 aagttagcat gactcacgcc gggcgtccag tttttagcga cggggcaccc gaacatgaaa
     7381 ttcccttacg ccaggttagc gtcgaggaac tctttcaact gacctttaga cagtgcgccc
     7441 actttggttg ccgccacttc accgtttttg aacagcagca gagtcgggat accacggatg
     7501 ccatatttcg gcgcagtgcc agggttttga tcgatgttca gttttgcaac ggtcagtttg
     7561 ccctgatatt cgtcagcgat ttcatccaga atcggggcga tcattttgca cggaccgcac
     7621 cactctgccc agaaatcgac gaggatcgcc ccgtccgctt tgagtacatc cgtgtcaaaa
     7681 ctgtcgtcag tcaggtgaat aattttatcg ctcatatata actccacagg aataagcctg
     7741 gcgtgttggt ttcgttgttg gtgtaacatt aaccaactaa aggttgactt tatttcaccg
     7801 gatacgcttt cgtaaagcaa tagtaagctg atattctacc acactatgag caaaacacat
     7861 ttaacagaac agaagttttc cgacttcgcc ctgcatccga aggttgtaga agcccttgaa
     7921 aaaaaagggt ttcataactg tacgcccatt caggcactgg cccttccgct gacgctggcg
     7981 ggtcgtgacg tagccgggca ggcgcaaacc ggtaccggga aaacgatggc gtttcttacg
     8041 tcaacgtttc attatcttct ctctcatcct gcgattgccg atcgcaaggt gaatcaaccg
     8101 cgtgccttaa ttatggcacc gacgcgtgaa cttgccgtgc agatccacgc cgacgcggaa
     8161 ccgctggcgg aagctaccgg cctgaagctg ggcctggctt acggtggtga tggctatgac
     8221 aaacagctga aagtgctgga aagcggcgtt gacattctga ttggcaccac gggtcgttta
     8281 attgactacg ccaagcagaa ccacattaac ctcggtgcca ttcaggtagt ggtactggac
     8341 gaagccgatc gcatgtacga tctaggcttt attaaagata tccgctggct gttccgccgt
     8401 atgccgcctg caaatcagcg cctcaacatg ctgttctccg ccacgctttc gtaccgggta
     8461 cgtgaactgg cgttcgagca gatgaacaat gccgaatata ttgaagtgga accggaacag
     8521 aaaacgggcc accgtattaa agaagagctt ttctaccctt ctaacgaaga aaaaatgcgt
     8581 ttgctgcaaa cgctgatcga agaagagtgg ccagacagag cgattatttt cgccaatacc
     8641 aaacaccgtt gtgaagagat ctggggccac ctggcggcag atggtcatcg tgtcggtttg
     8701 ttgacaggcg atgtcgcgca gaaaaaacgt ctgcgtattc ttgatgaatt tacccgtggc
     8761 gatctggata ttctggttgc caccgacgtt gccgcgcgtg gtttgcatat tccggcagtg
     8821 acgcacgtct ttaactacga tttacccgat gactgtgaag attacgttca ccgtattggt
     8881 cgtacaggtc gcgcaggcgc aagcggtcac tctatcagcc tggcgtgtga agagtatgca
     8941 ttgaatttgc ctgctattga gacctatatt ggtcactcaa ttccggtaag caaatacaat
     9001 ccggacgcat tgatgaccga tctgccaaaa ccgctgcgcc tcacgcgccc gcgcacaggc
     9061 aatggtccgc gtcgtactgg cgctccgcgt aatcgtcgtc gttcaggtta aataaaatgt
     9121 catactcaaa atcattcaaa ctgtatcaag atggcaagag aatgaatccc gatgagctta
     9181 ctgtagtaag tgattcgggt gaaagaatgc agccaacaca gagacagatt gaaggatgaa
     9241 gagtatgggt tccacctcgt cgctgtatgc agccattgat ctcggttcga atagttttca
     9301 tatgctggtt gtgcgcgagg tggctggaag catccagacg ctgacgcgaa ttaaacgcaa
     9361 agtgcgtctg gctgctggcc tgaatagcga aaatgacctc tctaatgaag caatggagcg
     9421 cggttggcaa tgtctgcgcc tgtttgctga acgtctgcaa gatatccctc cctcgcaaat
     9481 tcgcgttgtc gctacggcga cattacgact tgccgtcaat gcgggtgatt ttattgccaa
     9541 agcgcaggaa atcctcggtt gtccggtaca ggtgatcagc ggtgaagagg aagcgcgtct
     9601 gatttatcag ggcgttgctc acaccactgg tggtgccgat cagcgcctgg tggtggatat
     9661 aggcggtgcc agtactgaac tggtaaccgg cacgggtgca caaaccacct cgttgttcag
     9721 cctgtcgatg ggctgcgtca cctggctgga acgctatttt gccgatcgta atctggggca
     9781 agaaaatttt gatgctgcag aaaaagcggc acgcgaagtg ttacgtccgg ttgccgatga
     9841 attacggtat cacggctgga aagtgtgcgt tggtgcttcc ggcaccgtgc aggcgttaca
     9901 ggaaatcatg atggcacagg ggatggatga acgcattacc ctggaaaagt tgcagcaatt
     9961 gaaacagcga gccattcatt gcggtcggct ggaggaactg gagattgacg ggctgacgct
    10021 ggaacgtgca ttagtgttcc cgagtggtct ggcgatcctg atcgccattt ttaccgaact
    10081 gaatattcag tgtatgaccc tggcgggcgg tgcgctgcgt gaaggcctgg tctacggcat
    10141 gttgcatctg gccgtcgagc aggatattcg cagccgtaca ctgcgtaata ttcaacgccg
    10201 ctttatgatc gatattgatc aggcacagcg cgtagccaaa gttgcggcta acttcttcga
    10261 tcaggtggaa aacgaatggc atcttgaagc aataagccgc gatttgctca tcagcgcctg
    10321 tcagcttcat gaaatcggcc tgagcgttga cttcaaacaa gctccgcaac acgctgctta
    10381 tctggtgcgt aacctggatc ttcccggttt tacccccgca cagaagaaac tgctggcgac
    10441 gctactgctc aaccagacta acccggtcga tctctcatcg ctgcatcagc aaaatgccgt
    10501 accaccgcgc gtcgcagaac aactctgccg tttactccgc ctggctatca tttttgccag
    10561 ccgtcgccgt gacgatctcg tgccagagat gacattacag gctaaccatg aactgttgac
    10621 cttgacgctt ccgcaaggtt ggctaaccca acatccgctg ggtaaagaga ttattgctca
    10681 ggaaaaccag tggcagagct atgtccactg gccgctggaa gtgcattaat gagtaagtgc
    10741 cggatgcgat gctgacgcat cttctccggc cttgattatt tccctcgttt tgccgccatc
    10801 atcgctttca gattcgccag atggctttgc cctttctgca tccgttcttc ggcactgacc
    10861 actttgcgct cctgttccca aatcagatca tcctgcggca gctccagcaa aaagcggctc
    10921 ggctccgggc gcaccagttc gccgtactga cggcgttctt tacacagcgt aaaggtcaat
    10981 tccttctggg cgcgggtaat gccgacatag gccagccgcc gctcctcatc gatattatct
    11041 tcatcgatac tgctctggtg cggcaaaaac ccttcttcca taccgaccat gtagacataa
    11101 ggaaactcca gcccttttga cgcgtggaga gtcatcagtt gcacctgatc cagctcttct
    11161 tcactctcac cacgctccat catgtcgcgt aaagtaaagc gcgtcaccac ctgggtgagc
    11221 gtcatcggct catccagttc gctgccttcc agcatctccg tcatccagct aaacagttgg
    11281 ttgacgttct tcatgcgcat ttcggcagct ttcgtgctgg gcgatgtttc gtacagccag
    11341 gattcataat ccatgccgtg gatcagatcg cgcaccgcgg caatcggctc ccgctccgcc
    11401 agacgctgga tttctgccaa ccagtgagtg aagcgggtca atgcttcata accacgtccg
    11461 ctaagcgtct gactcaggcc catatcaaag ctggcggtaa acatgctttt attgcgcgtc
    11521 atcgcccact cacccagctt tttcagcgtc gccgggccaa tctctcgctt cggcgtgtta
    11581 acgatacgca gaaatgcgct gtcatcgtcc gggttagtca gcacgcgcag ataagccagc
    11641 aagtccttga tttcaggacg agagaaaaac gacgtaccac cagaaatttt gtacgggatg
    11701 cggttttgca tcaggaattt ttcaaacacc cgcgactgat agttaccgcg ataaagaata
    11761 gcgtaatctt tgtactgcgt tttattgacg aagtgatgcg caatcagttc accagtgacg
    11821 cgctcggctt catgttcttc gttattcgcg cttaaaactt ttagctctgt gccgtaaccc
    11881 agttcggaga acagacgctt ttcaaagacg tgcgggttat tggcgatcag gatgttcgcc
    11941 gctttcaaaa tacgtccgga agagcggtag ttctgctcaa gcttaatcac cttcagcgcc
    12001 ggaaaatcct ggctcagcag caccaggttt tgcggacgtg cgccgcgcca ggagtagatc
    12061 gactggtcat cgtcacccac cacggtaaag cgcgcgcggc tgcccaccag cagtttcacc
    12121 agttcatact ggctggtgtt ggtgtcctga tattcgtcca ccagcaggta gcgaattttg
    12181 ttctgccagc gctcgcggac ttcttcattg cgttgcagaa gtagcgtcgg caataaaatc
    12241 agatcatcga agtcgagcac attacaggct ttcaggtgtg catcatacag cccataacaa
    12301 tgggcaaaaa tacggtcccg ctcgccaatc gcactggctg ccgcctggga cggtgttttg
    12361 agatcgttct tccagttaga gatggtcgaa atcaactgtt gcaggagaac tttgtcatct
    12421 tcaatcagcc cttcggtcaa ctctttgagc aacgccagct gatcggtatc gtcaaacagc
    12481 gaaaagttcg ccttcatccc cagcgccgca tactcacgct tgatgatatc cagccccagc
    12541 gtgtggaagg ttgagatcat cagcccatgc gcctctttgc gacccagcgt ctgtccgaca
    12601 cgctctttca tctcgcgcgc cgccttattg gtaaaggtca ccgccgcgat atgtcgcgcc
    12661 tgataaccgc acccgcggat cagatgggcg attttattgg tgataacacg agttttaccg
    12721 gaacccgcac ccgccagcac caggcagggg ccggtaacga attcgacagc ttgttgttgg
    12781 ccggggttta gacgcatagg aaaatcgctc aatgaaagtc gggaggggaa aataacagcg
    12841 tggtagtata gccagcctca tccttaccac tcaaggcacg atcatggcaa aaacagcagc
    12901 agcactgcat atccttgtaa aagaagagaa actggctctg gatcttctcg tgcagattaa
    12961 gaacggtgcc gatttcggca agctggcgaa gaaacactcc atttgcccat caggcaaacg
    13021 cggcggtgat ttaggtgaat tccgccaggg tcagatggtt ccggcgttcg ataaagttgt
    13081 gttctcttgt ccggtactgg agccaaccgg cccgctgcac acccagttcg gttaccacat
    13141 cattaaggtg ctgtaccgta actaatagca aggccttctc cgggagaagg ccttgaactc
    13201 tgaatcgcta ttaacccgca acagcaatac gtttcatatc cgtcatatag ccgcgcagtt
    13261 tcttacctac ttgctcaatc gcatggctgc gaatcgcttc atttacatca cgcagctgcg
    13321 cgttatctac cgcaccttcc ggaatagctt tacccaaatc gcccggttgc agttctgcca
    13381 taaacggttt cagcagcggc acacaagcgt aagagaacag atagttaccg tactcggcag
    13441 tatcagagat aaccacgttc atttcataca gacgcttacg gacgatggtg ttggcaatca
    13501 gcggcagttc gtgcagtgat tcatagtaag cagattcttc gatgatgccg gaatcaacca
    13561 tggtttcgaa cgccagttca acgcccgctt tcaccatcgc gatcatcagt acgcctttat
    13621 cgaagtactc ctgctcgccg attttgcctt catactgcgg cgcggtttcg aaggcggttt
    13681 tgccggtctc ttcacgccag gtcagcagtt tcttatcatc gttggcccag tccgccatca
    13741 taccggagga gaattcgccg gagatgatgt cgtccatatg tttctggaac agcggtgcca
    13801 tgatctcttt cagctgttca gacaacgcgt aagcacgcag tttcgccggg ttagagagac
    13861 ggtccatcat cagtgtgata ccgccctgct tcagcgcttc ggtgatggtt tcccaaccga
    13921 actgaattag tttttctgcg tatgccggat cggtgccctc ttccaccagc ttgtcgaagc
    13981 acagcagcga gccagcctgc aacataccgc acaggatggt ttgctcaccc atcaggtcag
    14041 atttcacttc cgcaacgaag gacgattcca gcacgcccgc acggtgacca ccggttgctg
    14101 ccgcccatgc tttagcgatc gccatgcctt cgcctttcgg atcgttttcc gggtgaacgg
    14161 caatcagcgt cggtacgccg aatccacgtt tgtactcttc acgcacttcg gtgccagggc
    14221 atttcggcgc aaccatgact acggtgatgt ctttacggat ctgctcgccc acttcgacga
    14281 tgttgaaacc gtgcgagtaa cccagcgccg cgccgtcttt catcagtggc tgtacggtgc
    14341 gcactacatc agagtgctgc ttgtccggcg tcaggttaac caccagatcc gcctgtggga
    14401 tcagttcttc gtaagtaccc actttaaaac cattttcggt cgctttacgc caggacgcgc
    14461 gcttctcggc aatcgcttct ttacgcagag cgtaggagat atcgagacca gaatcacgca
    14521 tgttcagacc ctggttcaga ccttgtgcgc cacagccgac gatgactact tttttaccct
    14581 gaaggtagct cgcgccatcg gcgaattcat cgcgccccat aaagcgacat ttgcccagct
    14641 gtgccagctg ctgacgcaga ttcagtgtat tgaagtagtt agccatggtg attcctcgtg
    14701 atgttgtgct tcttattgtt cggttcgcgt ttgcgagatt gaattcacta tatgacagga
    14761 aatttattgc ggaaattgat atattcacaa cgtcacattg caatttctgc aacgtcaaca
    14821 ttgagggctg cgccattgga tttacgcgat ctgaaaacct tcctgcatct ggcggaaagc
    14881 cgccattttg gccgcagcgc gcgggcgatg cacgttagcc cgtccacgct ctcacggcag
    14941 attcagcgcc tggaagaaga tctcggccag ccgctgtttg tgcgcgataa ccgcacggtg
    15001 acgctgactg aagcgggcga agaactgcgc gttttcgccc agcaaacgct gttgcagtat
    15061 cagcagctgc gccacaccat cgaccagcaa gggccgttgc tctatggcga attacatatc
    15121 ttctgctcgg tgaccgccgc ttacagccat ctgccgccga ttctggatcg cttccgcgcg
    15181 gaacacccgt cggtggagat caaactcact accggtgatg cggcagatgc aatggaaaaa
    15241 gtcgtcacag gtgaagctga tctggcgatt gccgggaagc cagagactct gcccggcgcg
    15301 gtggcgtttt cgatgttgga gaatctggca gtggtgttga ttgcacccgc attgccctgc
    15361 ccggtgcgta atcaggtttc tgtcgaaaag ccggactggt caacggtacc gtttatcatg
    15421 gccaatcagg ggccggtgcg ccgccgcatt gagttgtggt tccgacgcaa taaaatcagt
    15481 aacccgatga tttacgccac ggttggcggg catgaagcga tggtatcgat ggtggcgctc
    15541 ggctgtggcg tggcattgtt gccggaagtg gtgctggaaa acagccccga accggtgcgt
    15601 aaccgcgtga tgattttaga gcgcagcgat gagaaaacgc cgtttgagct gggcgtgtgt
    15661 gcgcagaaaa agcggctaca tgagccgcta attgaggcat tctggaagat tttgccgaac
    15721 cattaacccg ccaaaaagaa cctgaacgcc gggttattgg tttcgtcgtg gcaatcgtag
    15781 cccagctcat tcagccgggt ttcgaaatcc ggttcatggt cgccaagttc gaacgccgcc
    15841 agtacgcgcc cgtagtcggt gccatggctg cgatagtgga acaaagaaat gttccagtgc
    15901 gtacccagcg tgttgaggaa gcgcagcagc gcgcccggtg attccgggaa ttcgaagctg
    15961 tagaggcgtt cctgcaacgg atgtgacgga cgcccgccga ccatatagcg cacatgcaac
    16021 ttcgccattt cgtcgtcgga gagatccacc acgctgtagc cgccgtcgtt gagcatctgc
    16081 aaaatttctt tgcgctcttc gaggccgcgg ctcaggcgca cgccgacaaa gatgcaggcg
    16141 tttttggcat cggcaaaacg gtagttgaac tcggtgaccg atcgcccgcc aagcagttgg
    16201 cagaatttga ggaagctgcc tttttcttcc ggaatggtca ccgccaacaa cgcttcacgc
    16261 tgttcgccca gttcgcagcg ttccgagacg tagcgcaggc cgtggaagtt cacgttggca
    16321 ccggaaagaa tatgcgccag ccgttcgccg cgaatgttgt gctgggcgat atattttttc
    16381 attcccgcca gcgccagcgc gccagagggt tccgccaccg cacgcacatc ttcgaataaa
    16441 tccttcatcg ccgcacagat cgcatcgcta tcgacggtga tgatgtcgtc gagatactcc
    16501 tggcataaac ggaaggtttc gtcaccgatg cgttttaccg cgacaccttc ggcgaacagt
    16561 cccacgcgtg gcagatcaac cggatgaccc gcatccagcg ctgctttcag gcaggcggag
    16621 tcttccgctt ctacggcgat cactttgatt tgcggcatca gttgtttgat cagcaccgct
    16681 acgcccgccg ccagaccgcc gccgccgact ggcacaaata cgcggtcgag atgggcgtcc
    16741 tgctggagca gttccagcgc cagcgtgcct tgcccggcaa tcaccatcgg atggtcgaac
    16801 ggcggcaccc aggtgaaccc ctgctgctgt gacagttcga tcgctttagc tttcgcttcg
    16861 tcaaagttcg cgccatgaag cagcacttcg ccgccgaatc cacgtaccgc atcgactttg
    16921 atgtcggcgg tggcggttgg cataacgatc agggccttca cgcctaaccg cgcagaagaa
    16981 aacgcgacgc cctgcgcgtg gttacccgca gaagcagtga tcacgccgtg cgctttttgt
    17041 tcttccgtca ggcccgccat catggcgtat gcgccgcgca gcttaaagct gtgcactggc
    17101 tggcgatctt cgcgcttcac cagaatgacg ttatcaagac gcgacgacag tttttccatt
    17161 ttttgtagcg gcgtaacctg cgccgcctcg taaaccggcg cgcgcagcac tgctcttaaa
    17221 tattcggcac cttccggagc accggacagg ggttgcgagt cagccattat taacccccca
    17281 gtttcgattt atcgcgcacc gcacctttgt cggcgctggt tgccaggctg gcgtaggcgc
    17341 acagggcaaa ggaaacctga cgttcacggt ttttcggcgt ccaggctttg tcaccccgag
    17401 cttcctgcgc ttcacgacgc gccgccagtt cggcatcgct tacctgtaac tgaatgccac
    17461 ggttcgggat gtcgatagcg atcaggtcac catcttcaat cagaccaatg ctgccgccgc
    17521 ttgccgcttc cggtgagacg tggccgatgg aaagaccaga ggtgccgcca gagaaacgac
    17581 cgtcggtgat cagcgcacag gctttaccga gacccattga tttcaggaag ctggttgggt
    17641 agagcatttc ctgcatgcca gggccgcctt tcgggccttc atagcggatc accaccacat
    17701 cgcccgcgac aactttgcca ccgaggatcg cttctaccgc gtcgtcctgg ctttcgtaca
    17761 ctttcgccgg gccggtgaat ttgaggatgc tgtcatcgac gcccgcggtt ttcacgatgc
    17821 agccgttttc agcaaaatta ccgtagagca ccgccaggcc gccgtctttg ctgtaggcgt
    17881 gttccagcga gcggatacag ccgtttgcgc gatcgtcatc gagagaatcc caacggcaat
    17941 cctgcgagaa tgcctgtgta gtacgaatgc ccgcagggcc tgcgcggaac atatttttta
    18001 ccgcgtcatc ctgggtcagc ataacgtcgt attgttccag agtttgcggc aacgtcaggc
    18061 caagtacgtt tttcacatca cggttcagta accccgcgcg atccagttca ccgagaatac
    18121 cgataacacc accagcacgg tgaacatctt ccatatggta tttttgggtg ctcggcgcaa
    18181 ctttgcataa ctgcgggact ttacgcgaaa gtttgtcgat atcactcatg gtgaagtcga
    18241 tttccgcttc ctgcgccgcc gccagcaggt gaagtaccgt gttggtagag ccgcccatcg
    18301 cgatatccag cgtcatggca ttttcaaacg ccgctttgct agcaatattg cgcggcagtg
    18361 cactttcgtc gttttgctcg tagtaacgtt tggtcaattc aacaatgcgt ttaccggcat
    18421 tgaggaacaa ctgcttacgg tccgcgtggg ttgccagcag cgagccgttg cccggctgcg
    18481 acaggcccag cgcttcggtc aggcagttca ttgagttagc ggtaaacatc ccggagcagg
    18541 agccgcaggt cggacacgcg gaacgttcaa cctgatcgct ctgggagtca gaaactttcg
    18601 ggtctgcgcc ctggatcatc gcatcaacca gatcgagttt gattatctga tcggacagtt
    18661 tggttttccc ggcctccatc gggccgccgg aaacaaagat caccggaata ttcaggcgca
    18721 gggaagccat cagcatcccc ggggtgattt tgtcgcagtt agagatgcag accatggcgt
    18781 cggcgcagtg ggcgttgacc atatactcaa cggaatcagc gatcagttcg cgagatggaa
    18841 gtgaataaag catccccccg tggcccatgg caatcccatc atccaccgca atggtgttga
    18901 actctttggc aacgccgcca gccgcttcaa tttgttcggc gaccagttta ccgagatcgc
    18961 gcagatggac gtgacccggt acaaattggg tgaacgagtt cacaaccgcg ataatcggct
    19021 tgccaaaatc ggcgtcggtc attccggtgg cgcgccacag cgcacgagca cccgccatat
    19081 tacgaccatg agtggtggtg gcggaacggt acttaggcat actttattta ctcccagtgt
    19141 ctgtctcgta aatgggacgg tgcgtgccgt cccatttttt gtatttattg attaacttga
    19201 tctaaccagc cccatttatc ttcggtttcg ccagtgaaga ggccgaagaa ggcttgctga
    19261 atgcgtttgg taaccgggcc acaacggcct tcgccaatct gaataccgtc tacgctgcgc
    19321 actggcgtga tttctgccgc cgtaccggac ataaagactt catccgccaa gtacagggat
    19381 tcgcgcgaca gcacctgctc acgcacttca attcccagct ctttcgccag tttgatgatg
    19441 gcatcacggg taatacccgg cagcgcggag gaggtgaacg gtggggtgaa cagcacacca
    19501 tctttcactt caaacaggtt ttcgcctgcg ccttcagaga tataaccgtt cacatccagc
    19561 gcgatacctt cctgataacc gtggcggcgc gcttcgctac ccaccagcag ggaagagaga
    19621 tagttaccac cggcttttgc cgcagttggg atggtgtttg gtgctgcgcg gttccaggag
    19681 gaaaccatcg catcgatccc ctgctccagc gcttctgcgc ccagatacgc tccccacggg
    19741 aaagcggcga taatcacatc ggttgagtat cccgctggcg ggtttactcc catgccaaca
    19801 tcaccgacga agatcagcgg acggatatag gcgctggtga gattgttttt gcggatcacg
    19861 tcacgacaag cttccatcag ctcatcaatg ctctgtgaaa ccgggaagcg atagattttg
    19921 gcggagtcat gcagacgctg catatgctca cgatggcgga atacaaccgg tcctttgtgt
    19981 gagtcgtagc aacggatgcc ttcaaaaacc gaggtgccat agtgcagcgc gtgcgacatc
    20041 acatgcacct tcgcgtcttc ccagcgaacc atctccccat tgaaccaaat gtaatcagct
    20101 ttcttcgtgg tcatttttat attccttttg cgctcaggcg cggatttgtt gtgatgtggt
    20161 tgtgctctgg cagatggcaa cgtgtgcgac gtccaccagt ttatttaact gactaaacag
    20221 taagtcgacc gaccgtgggc tggcaacggt caattcgata tttatatttt gtgcatcgct
    20281 ggcggcagcc atattcattg agcagacgtg gaaaccacga tgacgcacca cgcgtaaaac
    20341 acgttctaag gtttccggat tgaagcgagc cgatacattg acctgatgtt gcatcatgat
    20401 aatttctcca acatttctga attactggca cctggcggca ccagcggcca gacgttctca
    20461 agttcgtcga ttgagacatg aagcaggtat ggcccatcac tgttcagcat ggtgtcgagt
    20521 gccgcttcaa cctggtcttt acgggtgatg tgttggccag ggatgccgaa ggcgctggct
    20581 aacatgagga aatcggggtt atcggtaagg gtggtttcgc tgtatcgttc ctgaaaaaac
    20641 agttgctgcc attgtcgaac catccctaac cgttggttat cgagtaagac gattttcaac
    20701 ggtaactgct tgcgttttac ggtgcccagc tcttgcacat tcatcatgaa agagccgtca
    20761 ccggagatac agacaacggt atcgttcggt cgcgcgactt gtgcgccaac tgccgccggt
    20821 aaaccaaaac ccatggtacc taagccgctg gaggtgatga aattttccgg gcgagtgtgg
    20881 gcgatgtgct gcgcagccca catctggtgc tgccccacat ctgtggtcac gacgcaatcc
    20941 gcaggtttac gatccgacag ttgttttaac aacaacggag cgtagatagc gtcaccggga
    21001 tggtcgtaac gccaggaatg ttcatcacgc agctgcgcgc agtgttgctg ccagtcattg
    21061 atatttaacg gctgctgtaa tgctggtaac agagcattta aatcaccttg taatgccaca
    21121 tgtgcctgac gcagcttgtt catttctgcc gggtcgatat ccatatggat aacactggcg
    21181 tgtggtgcga aggtgttcag tttgccggtc acccggtcat caaaacgtgc acccacggcg
    21241 atcagcaggt cacactcctg caccgcgaag tttgccgctt tggttccgtg catccccagc
    21301 atgcccagat agtacggata atctgcttct actgcgccca gccctttcag cgtacaggtg
    21361 gcaggcattt ttgtggcagc gagaaattca cgcaaagccg gaactgcctg cgccatcccc
    21421 acgccaccgc caacgtacag catcggtttt tgcgcttttg ccagcatctg gcgcgcttgc
    21481 tcgacttcgg catgtgggaa agtcacttcg ttttcaacgg tggtgaacca cggttccaga
    21541 tcgccgctgg ctaactggat atcttttggg atatcgacca gaaccggacc aggacgacct
    21601 gagctggcaa cgtcgaatgc ttcagccatg atgcgcggca actcttccag agactgcacc
    21661 agaaagctgt gcttggtaca ggctaacgac aatcccagga catccacttc ctgaaatgcg
    21721 tcggtgccga taaacggtgc ggacacctga ccagtgatgg caacgacagg gatggaatct
    21781 aacagtgcgt ccgcaagccc ggttatcagg ttggttgcgc ccggaccaga cgtggcgata
    21841 catacgccag ttttaccggt agcacgggca taaccgatag ccgccattgc cgcaccctgc
    21901 tcatgtcggc acagcaagtg ctccacgccg ccgtcataca atgcatcgta aaccggcata
    21961 attgcgccac ccggataacc gaaaacggtg ttcacaccct gtgcccgcaa cgcatgtacc
    22021 acccactgtg cgccattcat agttagttcc ccgtcctgaa tcttgagaaa cagaattttg
    22081 tgctgttatt cattgtctgc tcctcggtta tgtttttaag gtcaaaaaaa cccccggacc
    22141 tttcggtgcg ggggtcttag ttcgttaagg cttgatctct aagcctttcc tcgtccaagt
    22201 gcagccccgc acggtgggat aataatcacc accacgctaa tcacgaccag gctaatcact
    22261 cgtagaaggg ctgtcatttt gtcttttctt gcatcttgtt cgaaggaatg cctaaagagt
    22321 taccatagat tttgcgaata gcacaagata tttttgggcc tatttttatg atggctttaa
    22381 taatttcaat agaaattgtt gttttttaaa aagaaaatag tgagtgaaaa aattcaattt
    22441 cagtataaat gttgttccct tcctcgtagt tcatcctttg tccgctgcat ttttgcgagc
    22501 atcattccac cgccagaaca actcaacttc acttgcacaa ataaacagga atcagccact
    22561 aaaattcacg aattagcggg ctacagcagc gatatcttat gcacataatg gtgactcaag
    22621 gagggtttat gtcactgtca attgttcata cccgcgcagc cctggaagta aatgcgcccc
    22681 caatcactgt tgaggtacat atcagtaaag gtctacccgg cttaacgatg gtgggcttaa
    22741 cagaaacaac ggtgaaagaa gctcgcgatc gcgtgcgcag cgccattatc aatagcggat
    22801 atgaatatcc ggcgaaaaaa atcaccatca accttgctcc agccgatctg ccaaaagaag
    22861 ggggacgata tgatttacct atcgccattg cgttgctggc ggcctcagaa cagcttacag
    22921 ccaataagtt agatgaatat gaattagtcg gagaactagc gcttacaggc gctctgcgtg
    22981 gcgttcccgg tgcaatctcc agtgcaactg aagctattaa gtcgggcaga aaaattatcg
    23041 tcgcgaaaga taacgaagat gaagtggggc taattaacgg tgaaggatgc ctgatagccg
    23101 atcatctgca ggctgtctgt gcgtttctgg aaggtaagaa cactctcgaa cgcccgaaac
    23161 caactgatgc agtatcccgg gcgctacaac atgatctcag tgatgttgtc ggtcaggagc
    23221 aaggaaaacg aggactggaa attaccgccg ctggtgggca caacctttta ctgattgggc
    23281 cgccgggaac aggtaaaaca atgctcgcca gccgtattaa tggcctgttg ccagatttaa
    23341 gcaatgaaga ggcactggag agcgctgcga tattaagtct ggtaaatgct gaatcagtac
    23401 aaaaacaatg gcggcagcgc ccgttccgct cacctcatca cagtgcgtcg ttaactgcga
    23461 tggtgggcgg tggtgcaatt ccagggccag gtgaaatttc gttggcgcat aacggcgtgc
    23521 tttttcttga tgagctacct gaatttgaac ggcgtacact ggatgccttg cgagagccga
    23581 ttgaatccgg gcagatccat ctttcacgca cacgagcaaa aataacctat ccagcccgtt
    23641 tccagcttgt tgcggcgatg aatcccagcc ctaccggaca ttatcaggga aaccataacc
    23701 gctgcacgcc agaacagaca ttacgttatc tcactggaga tcccattacc accccccggc
    23761 attttgagta aaacggtagt gccgggagaa agcagcgcca ccgttaaaca acgcgtaatg
    23821 gccgccagag agcgccaatt taagcggcag aataaactga atgcctggct ggatagtccg
    23881 gaaatacgcc aattctgcaa acttgagagc gaagatgcga tgtggttgga agaaacgctg
    23941 atccatctgg ggttatcgat tcgtgcctgg cagcggttac tgaaagttgc acgaaccatt
    24001 gctgatattg atcagtctga cattatcaca cgtcagcatt tgcaggaggc agttagctat
    24061 cgtgcgattg accgtttgct catccatctg caaaaactac tgacataaaa aaagggcatt
    24121 tcgccctttt tattaatcgt cagaatcggt gtagtcttca gcaccttcaa cctgcggttt
    24181 accgccggaa agggtgtgaa aacgttttgg acgcttaata cgcgtcatat acttggacca
    24241 cacgcgttct gcttctgtca ctggctcacg ttcgccacgg catactgcta cgaagagttt
    24301 ctcttcctcg gtaaccggct cgcgtttgcc aagatccaac tcattgaagg cataaccatg
    24361 acgctcaagc agttgtgcct ctttgatggt gaaatcacca tgacgagaga atccacgtgg
    24421 ataatgttta ttgtcgaaat atcgattagt cgtcgtaaag ctttccgcca tcctgcacgc
    24481 tcctaattct ttgaccgagc tagttatggc gcggagtatt agttacgctt gacagagtgt
    24541 aaaacaaaac atttaaatca taacgacaaa taattttgcg gagagcactg tggatacgga
    24601 attgttaaaa actttcctgg aagttagccg aacgcgtcac tttggtcgag cggctgaatc
    24661 gctctatctg acccagtcag cagtgagctt tcgaatcaga caactggaaa atcaactggg
    24721 tgtgaacctt ttcacccgcc acagaaacaa tatccgttta accgctgccg gtgaaaaact
    24781 actgccttat gcagaaacgc tcatgagcac ctggcaggcc gcccgcaagg aggtggcgca
    24841 tacctcacga cataacgagt tttctatcgg tgccagcgcc tcgttgtggg aatgtatgct
    24901 taatcagtgg ttgggacgct tgtatcaaaa tcaggatgcc catacaggct tacagttcga
    24961 agcgcgaatt gcccaacggc agtctctggt aaaacagctg catgaacgcc agcttgatct
    25021 tcttattacc actgaagcgc ccaaaatgga cgaatttagt agtcagttgc tggggtattt
    25081 cactttagcg ctttatacca gtgccccttc taaactaaag ggagatctta actatctgcg
    25141 actggagtgg gggccagatt ttcaacagca tgaggcaggt ttgatcggtg ctgacgaagt
    25201 tcccattctg acaaccagtt ctgctgaact ggcacagcaa cagattgcga tgcttaatgg
    25261 ttgcacctgg ctacccgtca gctgggcgcg taaaaaaggc ggcctgcata ccgttgtcga
    25321 tagcacaaca ctttcacggc cgctttatgc catatggctg caaaatagcg ataaaaatgc
    25381 gttgattcgc gatcttttga aaattaacgt gctggatgaa gtgtattaat ctgaatggct
    25441 ggcaaggatg ccggtagaag gatttacttc ggagaggatt atttcagata aaaaaaatcc
    25501 ttagctttcg ctaaggatga tttctggcag gggcggagag actcgaactc ccaacacccg
    25561 gttttggaga ccggtgctct accaattgaa ctacgcccct aattagggtg gcggaacgga
    25621 cgggactcga acccgcgacc ccctgcgtga caggcaggta ttctaaccga ctgaactacc
    25681 gctccaccga attcttttac aacc
//
LOCUS       DAZSNM010000033        25659 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZSNM010000033 DAZSNM010000000
VERSION     DAZSNM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25659
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.033"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHW42_001194"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHW42_001194"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR8943094.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="ACHW42_001195"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="ACHW42_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR8943095.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="ACHW42_001196"
     CDS             2630..3391
                     /locus_tag="ACHW42_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR8943096.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="ACHW42_001197"
     CDS             complement(3442..4326)
                     /locus_tag="ACHW42_001197"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR8943097.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHW42_001198"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHW42_001198"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR8943098.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="ACHW42_001199"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="ACHW42_001199"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR8943099.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="ACHW42_001200"
     CDS             6202..6474
                     /locus_tag="ACHW42_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR8943100.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="ACHW42_001201"
     CDS             6844..7920
                     /locus_tag="ACHW42_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR8943101.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="ACHW42_001202"
     CDS             7947..9326
                     /locus_tag="ACHW42_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8943102.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="ACHW42_001203"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="ACHW42_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="ACHW42_001204"
     CDS             10409..11245
                     /locus_tag="ACHW42_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFR8943103.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="ACHW42_001205"
     CDS             11250..12197
                     /locus_tag="ACHW42_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR8943104.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHW42_001206"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHW42_001206"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFR8943105.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="ACHW42_001207"
     CDS             13324..14082
                     /locus_tag="ACHW42_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR8943106.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="ACHW42_001208"
     CDS             14200..15558
                     /locus_tag="ACHW42_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8943107.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACHW42_001209"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACHW42_001209"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR8943108.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACHW42_001210"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACHW42_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR8943109.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACHW42_001211"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACHW42_001211"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR8943110.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="ACHW42_001212"
     CDS             19503..20054
                     /locus_tag="ACHW42_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR8943111.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="ACHW42_001213"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="ACHW42_001213"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR8943112.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="ACHW42_001214"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="ACHW42_001214"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR8943113.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="ACHW42_001215"
     CDS             23307..24347
                     /locus_tag="ACHW42_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR8943114.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..25657)
                     /gene="gdhA"
                     /locus_tag="ACHW42_001216"
     CDS             complement(24464..25657)
                     /gene="gdhA"
                     /locus_tag="ACHW42_001216"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR8943115.1"
                     /translation="MERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEA
                     TGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTV
                     VDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNE
                     LDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEM
                     AQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAML
                     AQGVI"
BASE COUNT         6517 a         5971 c         6625 g         6546 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaag ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatatgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgccg ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagta
//
LOCUS       DAZSNM010000034        25538 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZSNM010000034 DAZSNM010000000
VERSION     DAZSNM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25538
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.034"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            95..2248
                     /locus_tag="ACHW42_001217"
     CDS             95..2248
                     /locus_tag="ACHW42_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR8943116.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..4031
                     /locus_tag="ACHW42_001218"
     CDS             2301..4031
                     /locus_tag="ACHW42_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR8943117.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="ACHW42_001219"
     CDS             complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="ACHW42_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR8943118.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4750..5661
                     /gene="panE"
                     /locus_tag="ACHW42_001220"
     CDS             4750..5661
                     /gene="panE"
                     /locus_tag="ACHW42_001220"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR8943119.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5624..6214
                     /gene="yajL"
                     /locus_tag="ACHW42_001221"
     CDS             5624..6214
                     /gene="yajL"
                     /locus_tag="ACHW42_001221"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR8943120.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGHMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="ACHW42_001222"
     CDS             complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="ACHW42_001222"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR8943121.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7922..8164
                     /gene="xseB"
                     /locus_tag="ACHW42_001223"
     CDS             7922..8164
                     /gene="xseB"
                     /locus_tag="ACHW42_001223"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR8943122.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8164..9063
                     /gene="ispA"
                     /locus_tag="ACHW42_001224"
     CDS             8164..9063
                     /gene="ispA"
                     /locus_tag="ACHW42_001224"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR8943123.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9088..10950
                     /gene="dxs"
                     /locus_tag="ACHW42_001225"
     CDS             9088..10950
                     /gene="dxs"
                     /locus_tag="ACHW42_001225"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR8943124.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11005..11922
                     /gene="yajO"
                     /locus_tag="ACHW42_001226"
     CDS             11005..11922
                     /gene="yajO"
                     /locus_tag="ACHW42_001226"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR8943125.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="ACHW42_001227"
     CDS             complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="ACHW42_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR8943126.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="ACHW42_001228"
     CDS             complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="ACHW42_001228"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR8943127.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="ACHW42_001229"
     CDS             complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="ACHW42_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR8943128.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="ACHW42_001230"
     CDS             complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="ACHW42_001230"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR8943129.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="ACHW42_001231"
     CDS             complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="ACHW42_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR8943130.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="ACHW42_001232"
     CDS             complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="ACHW42_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR8943131.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16289..16828
                     /locus_tag="ACHW42_001233"
     CDS             16289..16828
                     /locus_tag="ACHW42_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR8943132.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17126..18010
                     /gene="tsx"
                     /locus_tag="ACHW42_001234"
     CDS             17126..18010
                     /gene="tsx"
                     /locus_tag="ACHW42_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR8943133.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18187..18534)
                     /gene="yajD"
                     /locus_tag="ACHW42_001235"
     CDS             complement(18187..18534)
                     /gene="yajD"
                     /locus_tag="ACHW42_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR8943134.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18663..19634)
                     /gene="secF"
                     /locus_tag="ACHW42_001236"
     CDS             complement(18663..19634)
                     /gene="secF"
                     /locus_tag="ACHW42_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR8943135.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19645..21492)
                     /gene="secD"
                     /locus_tag="ACHW42_001237"
     CDS             complement(19645..21492)
                     /gene="secD"
                     /locus_tag="ACHW42_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR8943136.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="ACHW42_001238"
     CDS             complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="ACHW42_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR8943137.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="ACHW42_001239"
     CDS             complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="ACHW42_001239"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR8943138.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23057..24127)
                     /gene="queA"
                     /locus_tag="ACHW42_001240"
     CDS             complement(23057..24127)
                     /gene="queA"
                     /locus_tag="ACHW42_001240"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR8943139.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24220..24793
                     /gene="acpH"
                     /locus_tag="ACHW42_001241"
                     /pseudo
     CDS             24220..24793
                     /gene="acpH"
                     /locus_tag="ACHW42_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24798..>25538)
                     /locus_tag="ACHW42_001242"
     CDS             complement(24798..>25538)
                     /locus_tag="ACHW42_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR8943140.1"
                     /translation="EAAKETQPEAYIVGEHFGDARQWLQADVEDAAMNYRGFTFPLWG
                     FLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKTLLGRDI
                     ARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQDTALFALYRRMI
                     VLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEVVLPASPFLNAV
                     QWQCKEGHGQLTDGILALPAISATVWMN"
BASE COUNT         6743 a         6498 c         6152 g         6145 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct gatgacagca ccagaatacg cttgttataa tagtcagtat tgttatgaag
     3361 acaaagtcag cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat
     3421 atttcacgac tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa
     3481 taagtaatac tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg
     3541 catctttcgc tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga
     3601 gaggactatt ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa
     3661 accgagtgtt ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta
     3721 taaatgccaa agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta
     3781 tgcataatct gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac
     3841 acattacctt tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg
     3901 tgggtaatga gcaactttat cagattggca aaatctacca taagttggaa attaaacctg
     3961 agccagagca acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg
     4021 caactcaata aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat
     4081 atccagaaag ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc
     4141 gcaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat
     4201 cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt
     4261 tctgagtggc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc
     4321 tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct
     4381 tggcacgcag aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga
     4441 tggttttgct ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg
     4501 actccacttc gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag
     4561 agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata
     4621 cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt
     4681 cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga
     4741 ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca
     4801 gcactttgta aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt
     4861 agtgtgaatc tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat
     4921 cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc
     4981 gatgccgtca aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac
     5041 aacggcatgg gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc
     5101 accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg
     5161 catatcggtc cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa
     5221 accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg
     5281 gccgtcaact gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta
     5341 cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc
     5401 gaagggcatc atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc
     5461 acagcagaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc
     5521 gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa
     5581 aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact
     5641 ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt
     5701 tcgcggcggt atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac
     5761 ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga
     5821 atatgacgtg atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac
     5881 tctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg
     5941 cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt ccgattggcc atatgaccgg
     6001 cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg
     6061 ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct
     6121 gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt
     6181 gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct
     6241 gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc
     6301 ttgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag
     6361 ccaggttttg ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga
     6421 aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat
     6481 atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg
     6541 ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc
     6601 gagaatactg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac
     6661 cgtcgggctt ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt
     6721 gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag
     6781 gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac
     6841 ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga
     6901 tgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc
     6961 gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct
     7021 gccaaagcgg ttccacagat aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc
     7081 gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga
     7141 aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg
     7201 gaaaccacca ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc
     7261 cagatgaaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt
     7321 taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg
     7381 cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt
     7441 ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat
     7501 acccggaata cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg
     7561 aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac
     7621 gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat
     7681 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
     7741 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
     7801 ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg
     7861 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
     7921 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
     7981 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
     8041 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
     8101 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
     8161 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
     8221 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
     8281 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt
     8341 ggcgttagca caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac
     8401 tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg
     8461 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
     8521 ctggcgttct cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt
     8581 tcgatgattt ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca
     8641 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
     8701 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
     8761 aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc
     8821 caggttcagg atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag
     8881 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
     8941 cggaagaaag cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa
     9001 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
     9061 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
     9121 gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc
     9181 tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc
     9241 tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt
     9301 gaccaattga tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc
     9361 cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc
     9421 gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt
     9481 ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc
     9541 gacggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgt
     9601 cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg
     9661 cttaacaacc atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc
     9721 gggaaaaaag ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa
     9781 catattaaag gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc
     9841 ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac
     9901 ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca
     9961 gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg
    10021 ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa
    10081 acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc
    10141 atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa
    10201 cacgcagtga cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc
    10261 tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag
    10321 ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac
    10381 cagggcgctt ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg
    10441 agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg
    10501 tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa
    10561 ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt
    10621 ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat
    10681 atgcgttttg tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa
    10741 gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa
    10801 gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt
    10861 attccgcaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg
    10921 gaagccaaaa tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa
    10981 attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc
    11041 gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca
    11101 tggacactgc cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata
    11161 aatttctttg ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc
    11221 gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc
    11281 gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc
    11341 ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac
    11401 acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt
    11461 tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa
    11521 cagcactgct gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa
    11581 gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc
    11641 ccgctggcgc gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct
    11701 gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag
    11761 cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg
    11821 ttgttaagta aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt
    11881 gatgagctgc tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa
    11941 acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg
    12001 tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga
    12061 tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg
    12121 atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata
    12181 tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc
    12241 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
    12301 acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc
    12361 accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc
    12421 gccagcgatc ccatcgagcc aggaacgatt gggcttaatc cacttccgaa tccgacagca
    12481 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac
    12541 aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag
    12601 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag
    12661 agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
    12721 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
    12781 agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
    12841 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
    12901 tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc
    12961 atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
    13021 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc
    13081 tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt
    13141 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
    13201 ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg
    13261 atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc
    13321 agcaggatcg atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc
    13381 cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac
    13441 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
    13501 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt
    13561 ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta
    13621 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
    13681 atcgcttcgt taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc
    13741 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag
    13801 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
    13861 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
    13921 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
    13981 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
    14041 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
    14101 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
    14161 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
    14221 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
    14281 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
    14341 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
    14401 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
    14461 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
    14521 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
    14581 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
    14641 taaattgggg ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt
    14701 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta
    14761 gcaatacgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac
    14821 ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
    14881 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
    14941 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
    15001 gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag
    15061 accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg
    15121 catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
    15181 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa
    15241 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
    15301 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc
    15361 cagcgacctg cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga
    15421 tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat
    15481 acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg
    15541 gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc
    15601 acccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc
    15661 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
    15721 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
    15781 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct
    15841 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac
    15901 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
    15961 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg
    16021 tggtgaaacg ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc
    16081 ccacgagacg agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac
    16141 ggcgtcctga ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa
    16201 ggcgcaacgc ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag
    16261 gctaattgcg acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct
    16321 gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca
    16381 cagcgtcagc acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac
    16441 tcagcaaaac aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa
    16501 aacaccggca agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac
    16561 gcctgataca gatggcacca cccttacact acgtattcag ggtgagtcga atgatccctt
    16621 gcccgcattc agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca
    16681 ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga
    16741 tgttgatatt ccgttacaat taaaggggat ttctgtggat cagttaggct ttgtacgtat
    16801 ccacgacatt caacctgtta tgcagtaaac gtatttcggg acgattttgt gcgtcccgca
    16861 acatcttccc cgtcattttg ttactctgct tacatcacct ggattgatag taaaagtttg
    16921 caacaagggc gaaagtcagt acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg
    16981 attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa
    17041 taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa
    17101 gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact
    17161 ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc
    17221 cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca
    17281 gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt
    17341 ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg
    17401 gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac
    17461 caatactgac cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta
    17521 cgacatgggt cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga
    17581 tatcgacact ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca
    17641 gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt
    17701 tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga
    17761 ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac
    17821 taataactct atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc
    17881 tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt
    17941 cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta
    18001 caacttctga ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt
    18061 gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa
    18121 ttcaatatat tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg
    18181 ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc
    18241 ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc
    18301 ttcggtatat ttcgaatgct catgatcgtg gcaatagaga cacaacaatt cccagttact
    18361 gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag
    18421 gttagaataa acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc
    18481 tttttcgcga tagccgcttt ctaaccgtgc atagtttttt gggatgatag ccatgaaaag
    18541 ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc
    18601 tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt
    18661 gattaaggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg
    18721 tgttcgcgct tcatacccag tttcagagcc aacgcagatg ctacgtagat ggaagatgca
    18781 gtgccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg
    18841 aagagataca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc
    18901 agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg
    18961 aagttttcac gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc
    19021 gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc
    19081 agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc ctgccgccag tcgccactca
    19141 aagcggaaac ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg
    19201 gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact caatacgctt cactgctgca
    19261 ttctgattgg tggattcgtt aatcaccttc agaacttggc tgcccagcac ctgaccgccg
    19321 gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc
    19381 agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc
    19441 ggtttttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag
    19501 ccgcgcacgc ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc
    19561 cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta
    19621 tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata
    19681 tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc
    19741 gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat
    19801 gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc
    19861 ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt
    19921 gatcagtacg ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgatacctgg
    19981 catactcagt gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc
    20041 aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat
    20101 agaaaccagc agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccag
    20161 ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg
    20221 cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct
    20281 gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac
    20341 cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat
    20401 cggtttgccg atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag
    20461 cgagatgtta acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg
    20521 gtcaccagtc agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt
    20581 cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt
    20641 taccagacgg aattccagcg ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc
    20701 ctgaataccg ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc
    20761 cgccacgcca agttggttta cacggttacg caggatatta atgttctgct gcaccgcata
    20821 ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg
    20881 gctgctaatc accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc
    20941 tttagcatcg cggaaagtga tgctcaggcc gtagttgttt tctttacgaa cagtggtata
    21001 cgggatgccc ttttcgcgca ggtcactgcg taggctatcg atattttgtt cctggagttt
    21061 gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag
    21121 gccgagcttc atcggctcag catgaatagc tgccagccag cgcggcgttg ccggggcaag
    21181 gttaagcgcc acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa
    21241 ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt
    21301 agcagttatt ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc
    21361 ggcgactccg cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag
    21421 cgcatacagc agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata
    21481 acggtttaac acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc
    21541 tttcggcagg acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag
    21601 cgcgatagca atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt
    21661 cgtcagaact tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg
    21721 cttctgctgt ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa
    21781 aatcaaagac atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat
    21841 aaaaaagctc attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt
    21901 ctcgcccctg acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatag
    21961 ccttgcgtaa acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc
    22021 gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa gtaagcgcgt gaataattgc
    22081 gacaggtgta gcaatcacac tcaggatcga gtgggccagt atcgctctta tatttcgcat
    22141 tgcggatttt caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg
    22201 gcattacgca gtcaaacata tcgataccgc gacgtacgcc ttcaaccagg tcttccggtt
    22261 taccaacgcc catcaggtaa cgcggtttgt ctgccggaat ttgcggacac acatgctcca
    22321 gaatacggtg catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat
    22381 caaagccgat atctaccaga cctttaacag atatatcacg taaatcttcg taaatactgc
    22441 cctgaattat accaaataac gcatttttgt taccaagact gtcaaaacgc tcacggctac
    22501 gcttcgccca acgcagagac atctccatag agcgttttgc gtaatcccag tcagcaggat
    22561 acggcgtaca ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca
    22621 tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc
    22681 cctgttcggt gattttacga atatcaccaa ggctgaagac ctggaagccg ccggagtcgg
    22741 taagaatcgg tcctttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt
    22801 cctggcccgg gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg
    22861 cttcaacttc ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac
    22921 aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg
    22981 tggtgtccag ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgttta
    23041 aaccagcgcc gcggaattac tccccgacgc gctcattaat tgcctgcgga ttgtacgtga
    23101 taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc ttctaccgcc gctttatagg
    23161 cattcatggt gtgttgataa ccggcaaagg ccgaaaccag cataatcagc gtcgactctg
    23221 gcaagtggaa gttcgtcacc agcgcatcga ccactttgta ctggaagccc ggatagataa
    23281 agatttgggt atcgtcaaag aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc
    23341 tttccagcga acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg
    23401 ccgccagtac cgcgtctacc acatcctgcg gtacttcagc gtattccgag tgcatgatgt
    23461 gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca
    23521 caaacgccat ctccacgcct ttggcgcgta atttttccag caaaggctcg tcaaaatgca
    23581 gaccagcggt cggggctgca accgcgcccg gtttttcgct ataaacggtt tgataaagtt
    23641 cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg cagcggcata tggccgatgc
    23701 tgttgaggat atccagtacc gagcgttcat cattaaattc gacttcaaac agtgcgccgt
    23761 ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag
    23821 gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct
    23881 caaccagcac ttcaatcttg ccgccgctgg ctttacgccc aaacagacgc gccgggatca
    23941 cgcgggtatt attaaaaacc agaagatcgc cggggttgag cttatcgagt aaatcggtga
    24001 aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga cagctactgc
    24061 gttcaggcat gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa
    24121 cgcgcatgac actgactcag actaaaataa gagtcgggta gtctagtgcc ggggcgctct
    24181 ccctgcaacc tttacccccc tttggataga atataatcga tgaatttttt agctcacctg
    24241 catttagccc atctcgcgga aagctcgctt tctggcaatt tactggctga tttcgtacgc
    24301 ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg gcattcatat gcatcgacgt
    24361 attgactgac aatctgccgg aagtccgcga agcacaggag tggtttcgta gtgaaacgcg
    24421 ccgcgttgcg cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc
    24481 gcagctgtcg ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat
    24541 gacgattttg ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga
    24601 gcagtggctg gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc
    24661 aagccgccgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta
    24721 tgacgccctc gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca
    24781 caaggcgtta taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga
    24841 atcccgtcag tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagaaac
    24901 ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt tgattgccac cagtacacgt
    24961 tgctgattca gcacgcggac aaataccact acgttatctt ccgcatacag cacctgacag
    25021 ccgccacgac gcagcgcctg acttttctta cgcagcacaa tcattcgccg gtacagcgcg
    25081 aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg
    25141 tttttaccat ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg
    25201 aacagccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat
    25261 cgcgcagtat cgtggctatc gagctgatta aacatacgta attgttgttg atgagaaagc
    25321 cctgcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatca
    25381 taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc
    25441 atggcagcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg
    25501 acaatatacg cttccggctg ggtttctttc gccgcttc
//
LOCUS       DAZSNM010000035        25423 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZSNM010000035 DAZSNM010000000
VERSION     DAZSNM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25423
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.035"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..188
                     /locus_tag="ACHW42_001243"
     CDS             <1..188
                     /locus_tag="ACHW42_001243"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8943141.1"
                     /translation="KYQNFYELLIQFLNIERWIWHVKAGANATPEVAYSLWRFSDIIG
                     KFDFASAGVFSWNLPPD"
     gene            167..1402
                     /gene="moeA"
                     /locus_tag="ACHW42_001244"
     CDS             167..1402
                     /gene="moeA"
                     /locus_tag="ACHW42_001244"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR8943142.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            1402..2151
                     /gene="moeB"
                     /locus_tag="ACHW42_001245"
     CDS             1402..2151
                     /gene="moeB"
                     /locus_tag="ACHW42_001245"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR8943143.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(2330..2992)
                     /gene="fsa"
                     /locus_tag="ACHW42_001246"
     CDS             complement(2330..2992)
                     /gene="fsa"
                     /locus_tag="ACHW42_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR8943144.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            3123..4022
                     /locus_tag="ACHW42_001247"
     CDS             3123..4022
                     /locus_tag="ACHW42_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR8943145.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            4028..6460
                     /locus_tag="ACHW42_001248"
     CDS             4028..6460
                     /locus_tag="ACHW42_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR8943146.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            6606..7421
                     /gene="ybiV"
                     /locus_tag="ACHW42_001249"
     CDS             6606..7421
                     /gene="ybiV"
                     /locus_tag="ACHW42_001249"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR8943147.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            7573..8838
                     /locus_tag="ACHW42_001250"
     CDS             7573..8838
                     /locus_tag="ACHW42_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR8943148.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(9079..10671)
                     /locus_tag="ACHW42_001251"
     CDS             complement(9079..10671)
                     /locus_tag="ACHW42_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR8943149.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            10890..11810
                     /gene="ldtB"
                     /locus_tag="ACHW42_001252"
     CDS             10890..11810
                     /gene="ldtB"
                     /locus_tag="ACHW42_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR8943150.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(11868..12986)
                     /locus_tag="ACHW42_001253"
     CDS             complement(11868..12986)
                     /locus_tag="ACHW42_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR8943151.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(12983..13450)
                     /gene="mntR"
                     /locus_tag="ACHW42_001254"
     CDS             complement(12983..13450)
                     /gene="mntR"
                     /locus_tag="ACHW42_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR8943152.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            13636..13764
                     /gene="mntS"
                     /locus_tag="ACHW42_001255"
     CDS             13636..13764
                     /gene="mntS"
                     /locus_tag="ACHW42_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR8943153.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            14036..15619
                     /gene="opgE"
                     /locus_tag="ACHW42_001256"
     CDS             14036..15619
                     /gene="opgE"
                     /locus_tag="ACHW42_001256"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR8943154.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(15667..16182)
                     /gene="ompX"
                     /locus_tag="ACHW42_001257"
     CDS             complement(15667..16182)
                     /gene="ompX"
                     /locus_tag="ACHW42_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR8943155.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(16235..16300)
                     /locus_tag="ACHW42_001258"
     CDS             complement(16235..16300)
                     /locus_tag="ACHW42_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8943156.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            16535..17422
                     /gene="rhtA"
                     /locus_tag="ACHW42_001259"
     CDS             16535..17422
                     /gene="rhtA"
                     /locus_tag="ACHW42_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR8943157.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            17721..18247
                     /gene="dps"
                     /locus_tag="ACHW42_001260"
                     /gene_synonym="pexB"
                     /pseudo
     CDS             17721..18247
                     /gene="dps"
                     /locus_tag="ACHW42_001260"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
     gene            18651..19397
                     /gene="glnH"
                     /locus_tag="ACHW42_001261"
     CDS             18651..19397
                     /gene="glnH"
                     /locus_tag="ACHW42_001261"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR8943158.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            19536..20195
                     /gene="glnP"
                     /locus_tag="ACHW42_001262"
     CDS             19536..20195
                     /gene="glnP"
                     /locus_tag="ACHW42_001262"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR8943159.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            20192..20914
                     /gene="glnQ"
                     /locus_tag="ACHW42_001263"
     CDS             20192..20914
                     /gene="glnQ"
                     /locus_tag="ACHW42_001263"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR8943160.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            21175..23399
                     /gene="ybiO"
                     /locus_tag="ACHW42_001264"
                     /pseudo
     CDS             21175..23399
                     /gene="ybiO"
                     /locus_tag="ACHW42_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(23396..24322)
                     /gene="rlmF"
                     /locus_tag="ACHW42_001265"
     CDS             complement(23396..24322)
                     /gene="rlmF"
                     /locus_tag="ACHW42_001265"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR8943161.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            24598..24858
                     /gene="mcbA"
                     /locus_tag="ACHW42_001266"
     CDS             24598..24858
                     /gene="mcbA"
                     /locus_tag="ACHW42_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR8943162.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            25123..>25423
                     /locus_tag="ACHW42_001267"
     CDS             25123..>25423
                     /locus_tag="ACHW42_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR8943163.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         6170 a         6671 c         6412 g         6170 t
ORIGIN      
        1 ataaatatca gaatttttat gaattactca ttcaattttt gaatatagaa agatggatat
       61 ggcatgtaaa ggcaggagcc aacgctacgc ccgaggtggc atacagtcta tggcgttttt
      121 ctgacataat aggcaaattc gattttgcct ccgcaggagt gttttcatgg aatttaccac
      181 cggattgatg tcgctcgaca ccgcgcttaa tgagatgctt tctcgcgtta ccccactgac
      241 cacccaggaa acgctgccac tggttcagtg ttttggtcgt attctggcga gcgatgtcgt
      301 ttcgccactg gatgtcccgg ggtttgataa ctccgctatg gacggctacg cggtgcgttt
      361 agccgatatt gcctccgggc aaccgctgcc cgttgccggt aaatcctttg ccggtcagcc
      421 ataccatggt gaatggcctg cgggtacctg cattcgtatt atgaccggtg cgccggtgcc
      481 ggaaggctgc gaagcggtgg tgatgcagga gcagactgaa caaacggaca atggcgtgcg
      541 ttttactgct gaagtgcgta gcgggcaaaa tattcgccgt cgcggtgaag atatctctgc
      601 aggtgcggtt gttttcccgg cgggaactcg cctgactacc gcagagctgc cagtaattgc
      661 ttcactgggg attgccgaag ttccggtgat tcgtaaagtg cgtgtagcgc ttttttctac
      721 cggtgatgaa ctccagttgc ccggtcagcc gctgggcgac ggccaaatct acgacaccaa
      781 ccgtctcgcc gtacacctga tgttagaaca gctgggttgc gaggtaatca acttagggat
      841 tatccgcgac gatccacatg ccctgcgcgc cgcatttatt gaagccgaca gccaggcgga
      901 tgtagtgatc agttccggcg gtgtttcagt gggtgaagcg gattacacca aaatgattct
      961 tgaagagctg ggggagatcg ccttctggaa gctggcgatt aaaccaggta aaccgttcgc
     1021 gttcggtaaa ctcagcaata gctggttctg cggcctgccg ggcaacccgg tttcagcgac
     1081 gctgaccttc tatcaactgg tacagccttt gctggcaaaa ctaagcggca acaccgccag
     1141 cggcctgccc gcgcgccagc gcgtacgcac agcgtcccgc ctgaaaaaaa cgccaggacg
     1201 tcttgatttc cagcgcggcg tgctgcaacg caacgccgac ggtgaacttg aagtgacgac
     1261 caccggacat cagggttcac atatatttag ctcctttagt ctcggcaact gctttatcgt
     1321 gctggaacgc gatcgcggca atgtggacgt gcgcgaatgg gtggaagtag aaccgtttaa
     1381 cgcgttgttc ggaggcctgt aatggcggaa ctcagcgatc aggagatgct gcgctacaac
     1441 cggcaaatca ttctgcgcgg ttttgatttt gacggtcagg aggcgctaaa agattctcgt
     1501 gtgctggtag tcggcctcgg tggactcggc tgcgcagctt cgcagtatct ggcaagcgcc
     1561 ggtgtcggca acctgacgct gctcgacttc gacacggttt cgctctcgaa tctgcaacgc
     1621 cagacactgc acagtgatgc cacggtcggg caaccgaagg tggaatccgc ccgtgacgcc
     1681 ctgacgcgga tcaacccaca tatcgcgatt acgccagtca atgcactgct agatgacgca
     1741 gaacttgcag cattgattgc tgaacacgat ctggtgctcg actgtacgga taacgttgcg
     1801 gtacgtaatc aactgaacgc aggctgtttt gccgcgaagg tgccgctggt ttccggcgcg
     1861 gcaattcgta tggaagggca aatcacggtc ttcacttatc aggacggcga accgtgctat
     1921 cgctgcctta gccgtttgtt tggcgagaac gcgttaacct gcgtagaagc aggcgtgatg
     1981 gcaccgttga taggcgtaat tggttcgttg caggcaatgg aagcgatcaa actgctggca
     2041 ggttatggca aaccggccag cgggaaaatc gtcatgtacg atgcgatgac ctgtcagttt
     2101 cgcgaaatga aactgatgcg caatccgggg tgtgaggtgt gcgggcagta attgccacct
     2161 ccaagcctga taagacgcga caagcgtcgc atcaggcatt tgctccagac gccagatgcg
     2221 gcgtgaacgc cttatctggc ctacggttcg ggcacagatt tccaagcgcg gcaggcatcg
     2281 gtgcactccg ccacgtcatc agcaatttat gaggatgcag aacgtccggt taaatcgacg
     2341 ttctgccaaa cgctccctgc cagtcctgct caaacttcgt cacagcggct tccaccgccg
     2401 gatagctaat catctgttgt gccacatcca gtggcagagt aattgattca catccggcca
     2461 gtaagcagtc cagcgcctga cgcggggttt tgaaactcgc tgccagcact ttcgcctgcg
     2521 gcgcatgcat tttcaataac tggtgtaagt cggtcacagt ctgaatgccg ctaccgccct
     2581 gagcatcaat acgattaacg taaggcgcaa catattccgc acctgccagc gccgacagca
     2641 gcccttgtgc cgcgccatat accgcggttc ccagcgtcgg aatcccttcc gcttttaaca
     2701 tcttaatcgc tgccagcccc tcggctgtca ccggaacttt caccacgata tccgcaataa
     2761 tagaacgcag cttaagcgcg tcattcacca tcccttcagc agtggtagcc attacctggg
     2821 caaacagacg cccctgaccg cccatcgctt catgaagttg cggaagcaca acatccagcg
     2881 gttttttacc cgcggcgata atgcttgggt tagtggtcac acccgccagc ggaaaaatac
     2941 gtgacagcgc cttcaccgca acaacgtctg aagtatccag atacagttcc ataaccatcc
     3001 tcaaaatgta ctttttgtct gaatgtagac tatcacggga aaactctctg aacagttgac
     3061 gcacatcaat ctaactttca ttcgaaagta ttttaatgtt tgaacgaaac agagaggcaa
     3121 ctatgatttt caatattcag cgctactcga cccatgatgg ccccggtatc cgcacggtcg
     3181 tatttcttaa aggctgttcg ctgggctgcc gctggtgtca gaacccggaa agccgcgccc
     3241 gtacgcagga tctgctgtat gacgcacgac tgtgtctgga aggctgcgag ctgtgcgcta
     3301 aggccgcgcc ggaagtgatt gagcgcgcgc tgaatggttt gcttattcat cgggaaaagt
     3361 taaccccgga gcatctgacg gcgttaaccg actgctgtcc gacacaggcg ttaaccgtgt
     3421 gtggtgaagt gaaaagcgtt gaggagatca tggcgaccgt tctgcgcgat aaaccgtttt
     3481 acgatcgcag cggcggcggc ttaacgcttt cgggtggtga acccttcatg cagccggaaa
     3541 tggcgacggc gctgctgcaa gcctgccacg aggcaggcat tcatactgcg gtagaaacct
     3601 gtctgcatgt gccgtggaaa tatatcgccc cttctctgcc ctatatcgat ctgtttcttg
     3661 ccgatttaaa acacgttgcc gacgcgccgt ttaaacagtg gaccgacggt aacgccgcca
     3721 gggtgctgga taacctgaaa aaactcgccg cagcgggcaa aaaaatcatt atccgcgtgc
     3781 cgctgattca gggctttaat gccgacgaaa cctctgtaaa agccattacc gattttgccg
     3841 ccgacgagct gcacgttggc gaaattcatt ttctgcccta ccacacgctg ggcatcaaca
     3901 aatatcactt acttaatctg ccctatgacg ccccggaaaa accgcttgat gcgccagagc
     3961 tgctcgattt tgcccagcag tatgcctgcc agaaagggtt aaccgcgacc ttacgaggat
     4021 aacaaccatg accacactga aactggacac gctcagcgac cgcattaaag cgcacaaaaa
     4081 tgcgctggtg catattgtga agccgccagt ctgtaccgag cgcgcgcagc actataccga
     4141 gatgtatcaa caacatctcg ataagccgat cccggtacgt cgcgcgctgg cactggcgca
     4201 tcacctggcg aatcgcacca tctggatcaa acacgatgag ttgatcattg gcaaccaggc
     4261 aagcgaagtt cgcgccgcgc cgatcttccc ggaatatact gtctcgtgga tcgaaaaaga
     4321 gattgatgat ctggcagatc gtcccggtgc tggctttgcg gtgagcgaag agaacaaacg
     4381 tgttctgcat gaagtgtgcc cgtggtggcg cggtcagacg gtacaggatc gctgctatgg
     4441 catgtttacc gatgagcaaa aaggtctgct ggcgaccggc atcattaaag cggaaggcaa
     4501 tatgacctcc ggcgatgcgc acctggcggt caatttcccg ctgctgctgg aaaaagggct
     4561 tgatggtctg cgtgagaaag tagcggaacg tcgctcgcgc atcaatctga cggtgctgga
     4621 agatttgcac ggcgaacagt tcctgaaagc gattgatatc atgctggtgg cagtcagtga
     4681 acacattgaa cgtttcgctg ccctggcgcg tgcaatggcc gcgaccgaaa cccgcgaaag
     4741 ccgtcgcgat gaactgctgg cgatggcaga aaactgcgat cttatcgccc accagccgcc
     4801 gcagactttc tggcaggcgc tgcaactgtg ttacttcatc cagttgattt tgcagattga
     4861 atctaacggt cactcggtgt cgtttggtcg tatggatcag tatctctacc cgtactatcg
     4921 ccgcgacgtt gaactgaacc agacgctgga tcgcgaacac gccatcgaat tgctgcatag
     4981 ctgctggttg aaattgctgg aagtgaacaa aatccgctcc ggctcccact caaaagcctc
     5041 tgcgggaagt ccgctgtatc agaacgtcac cattggcgga caaaatctgg ttgatggtca
     5101 accaatggac gcggtgaatc cactctctta cgcgatcctc gaatcctgcg gtcgcctgcg
     5161 ttccactcag cctaacctca gcgtgcgtta ccatgcagga atgagtaacg atttcctcga
     5221 cgcctgcgta catgtgatcc gctgcggctt cgggatgccg gcattcaaca acgacgaaat
     5281 cgtgatcccg gaatttatta aactcggtat tgaaccgcag gacgcttatg actacgcagc
     5341 gattggttgt atagaaaccg ccgtcggtgg caaatggggc tatcgctgta ccggcatgag
     5401 ctttatcaac ttcgcccgcg tgatgctggc ggcgctggaa ggcgggcgtg atgccaccag
     5461 cggcaaagtg ttcctgccac aagaaaaagc gttgtcggca ggtaacttca acaacttcga
     5521 tgaagtgatg gacgcgtggg atacgcaaat ccgttactac acgcgtaaat caatcgaaat
     5581 cgaatatgtc gtcgacacca tgctggaaga gaacgtgcac gatattctct gctcggcgct
     5641 ggtggatgac tgtattgagc gagcgaaaag tatcaagcaa ggcggcgcga aatatgactg
     5701 ggtttctggc ctgcaggtcg gtattgccaa cctcggcaac agcctggcgg cagtgaagaa
     5761 actggtgttt gaacagggcg cgattggtca gcaacagctg gctgccgcac tggcggatga
     5821 cttcgatggc ctgactcatg agcaactgcg tcagcggctg attaacggtg cgccgaagta
     5881 cggcaacgac gatgataccg tcgatacgct gctggctcgc gcttatcaga cctatatcga
     5941 cgaactgaaa cagtaccata acccacgcta cggtcgtggt ccggttggcg gcaactatta
     6001 cgcgggtacg tcgtctatct ccgctaacgt accgtttggc gcgcaaacta tggcaacgcc
     6061 ggacgggcgt aaagcccaca ccccgctggc agaaggcgct agtccggcct ctggtactga
     6121 ccatctcggc cctactgcgg tcattggctc agtgggtaaa ctgcctacgg cagcgattct
     6181 cggcggcgtg ttgctcaacc agaaactgaa tccggcaacg ctggagaacg aatctgacaa
     6241 gcagaaactg atgatcctgc tgcgtacctt ctttgaagtg cataaaggct ggcatattca
     6301 gtacaacatc gtttcccgcg aaacgctgct ggaggcgaaa aaacatccgg atcagtaccg
     6361 cgatctggta gtgcgtgtcg cgggttattc cgccttcttc accgcgctct ctccagacgc
     6421 tcaggacgat atcatcgccc gtactgaaca tatgctgtaa tctcttcggc ccggctgcgg
     6481 cgtcgccggg ctaaatcatt cacatcatca atttcatcct tactttcatt cgaaatataa
     6541 tttgtgctct gcgtcacatt gttattacac tgttacggtt tcattttcga gccaggagca
     6601 cacctatgag cgtaaaagtt atcgtcacag atatggacgg tacttttctt aacgacgcca
     6661 aaacgtacaa ccaaccacgt tttatggcgc aatatcagga actgaaaaag cgcggcatta
     6721 agttcgttgt cgccagcggt aatcagtatt accagcttat ttcgttcttt cctgagctaa
     6781 aggatgagat ctcttttgtc gcggaaaacg gcgcactggt ttacgaacat ggcaagcaac
     6841 tgttccacgg cgaactgacc cgacatgaat cgcggattgt tattggcgag ttgctaaaag
     6901 ataagcaact caattttgtc gcctgcggtc tgcaaagtgc atatgtcagc gaaaatgccc
     6961 ccgaagcatt tgtcgcactg atggcaaaac actaccatcg cctgaaacct gtaaaagatt
     7021 atcaggagat tgacgacgta ctgttcaagt tttcgctcaa cctgccggat gaacaaatcc
     7081 cgttagtgat cgacaaacta cacatagcgc tggatggcat tatgaaaccc gtcaccagtg
     7141 gttttggctt tatcgacctg attattcccg gtctacataa agcaaacggt atttcgcggt
     7201 tactgaaacg ctgggatctg tcaccgcaaa atgtggtagc gattggcgac agcggtaacg
     7261 atgcggagat gctgaaaatg gcgcgttatt cctttgcgat gggcaatgct gcggaaaaca
     7321 ttaaacaaat cgcccgttac gctaccgatg ataataatca tgaaggcgcg ctgaatgtga
     7381 ttcaggctgt gctggataac acatcccctt ttaacagctg acctctctac cggagccatg
     7441 cgctccggtt ttcccttctt tttacatcct gtacatcaca tcaaatcatt aaacttgcat
     7501 taacttattt ataacttgta ttgtcacatg caaattagca tagtttcgaa tgaaagttaa
     7561 tgcaaggtgg ttatggcttc tacttttacc agcgacacat tgcctgccga tcacaaagca
     7621 gctatccgtc agatgaagca cgcgctgcgg gcgcagcttg gcgatgtcca gcagatcttt
     7681 aatcagctaa gcgatgacat tgccacgcga gtggctgaaa tcaacgcact caaagcacag
     7741 ggcgatgccg tctggccggt gctgtcttat gccgatatca aagcaggtca tgttactgca
     7801 gagcagcgcg aacagattaa acgtcgcggt tgtgcggtga taaaaggcca tttcccccgc
     7861 gaacaagcgc taggctggga tcagtcgatg ctggactatc tgaaccgcaa ccgctttgac
     7921 gaggtctaca aaggccccgg cgataatttc ttcgggacgc tcagcgcttc acgtcccgag
     7981 atttacccca tctactggtc gcaggcgcaa atgcaggccc gccagagtga agaaatggcg
     8041 aatgcgcagt cgtttctcaa tcgtctgtgg acatttgaaa gtgatggaaa gcaatggttt
     8101 aacccggatg tgagcgtcat ctaccctgac cgtatccgcc gccttccgcc cggaacgacc
     8161 tccaaaggtc ttggagcgca taccgactcc ggggcgctgg aacgctggct gcttccagcg
     8221 tatcagcacg ttttcgccaa cgtctttaat ggcaatctgg cgaaatacga tccctggcat
     8281 gcggcacatc gtacggaagt tgaagagtac acggtggaca acaccaccaa atgttccgtg
     8341 tttcggacat tccagggctg gactgcgctc tcggatatgc tgcctggtca ggggttgctg
     8401 cacgtcgtgc ccattcctga agccatggcg tacgtactgt tacgtccgct gcttgatgat
     8461 gtgccggagg atgaactgtg cggcgtagcg cccggaagag tgttgccggt atcagagcaa
     8521 tggcatccac tgttaattga ggcgttaacc agcattccaa aactcgaggc cggagactcc
     8581 gtctggtggc actgcgacgt catccattcc gttgcccccg ttgaaaatca acagggttgg
     8641 ggcaacgtga tgtacattcc tgcggcaccg atgtgcgaga aaaatcttgc ctacgcgcac
     8701 aaggtgaagg ccgcactgga aaaaggcgca tcgccgggcg acttcccgcg cgaggactat
     8761 gaaacaaact gggaaggacg ctttacgctt gccgacctca acattcacgg taagcgagcg
     8821 ttgggcatgg atgtttgaga gttaatggaa gtgaattgat gtgggattaa gaaaatcgat
     8881 tcgacgttac tcagattgct gacaagtgcg ctttcttaat gcctgatgcg gcgttaacgc
     8941 cttatccggc ctacaaagtc ctgcagattc aatatattac aggtactgcg taggcctgat
     9001 aagcgtagcg catcaggcta ttttgcattt gtcatcagcc gtcgtcaccc cgacagtatc
     9061 ggggtgacgg ataatcactt actcgatccc tttactacgc aggtaatctt cgtaattacc
     9121 gctaaagtcg atcacgcgtt ccggggtgat ttccagaatg cgggtcgcca gggagcttac
     9181 gaactcacgg tcgtgtgaaa caaagatcag cgtgccctga tacagttcca gcgccatgtt
     9241 cagtgactcg atggattcca tatccaggtg gttggtcggt tcgtccatga tcagaatgtt
     9301 cggcttctgc atcattaact taccaaacag catccgccct ttttccccac cggaaagcac
     9361 tttagctggc tttttgatgt cgtcctggct gaacagcaaa cgaccgagaa tgctgcgtac
     9421 cgcctgctcg tcatcgcctt cctgcttcca ctgactcatc cattcgaaca cggtcagatc
     9481 attttcaaac tcatattcgt ggtcctgggc gtagtaacca atgcgcgcgt tttcagacca
     9541 ttttacggtg ccgctgtccg gttgcagatc gcccaccagc gttttcagca gcgttgattt
     9601 accgacgccg ttggtaccca gtaccgcaag tttttcaccg acctccagca gcaggttgag
     9661 atttttaaac agtggaccgt tatcaaaccc tttggtcaga ccttccactt ccagcgcgtt
     9721 acggaacagt ttcttatcct gttcaaaacg gataaacggg ttctgacggc tggaggcttt
     9781 cacctcttcc agtttgattt tatcaatctg gcgcgcgcgc gaagttgcct ggcgagattt
     9841 cgaggcgttg gcgctaaagc ggctaacgaa agattgcaac tcagcaatct gcgctttctt
     9901 cttggcgtta tcggccagca gacgttcacg cgcttgggtc gccgccgtca tgtactcatc
     9961 gtagttaccc ggataaacgc gcagctcgcc gtaatccaga tccgccatgt gggtacagac
    10021 catgttaagg aagtgacggt cgtgcgagat gatgatcatg gtgctgtcac gctcgttcag
    10081 cacctgttcc agccagcgaa tggtgtcgat gtcgaggttg ttggtcggtt cgtcgagcag
    10141 gagaatatcc ggctcagcaa acagcgcctg cgccagaagc acacgcagct tccagccagg
    10201 agcaacttca ctcatcgggc cgtagtgctg ttccactgga attcccacgc caagcaacag
    10261 ttcaccggcg cgagcttccg cagagtaacc gtccatttcg ccgtatttca cttccagatc
    10321 ggccacttta tagccgtctt cttcactcat ttccggcaaa gcatagatgc ggtcgcgctc
    10381 ctgcttcact tcccacaact ctttatgccc catgatcacc gtatccagca cagtgaactc
    10441 ttcaaaggca aactgatcct gacgcagctt accaatgcgc tcgttgggat cgagggaaac
    10501 gttacccagc gtcggctcaa ggtcgccgcc gagaatcttc ataaaggtgg atttaccact
    10561 accgttcgcg ccaatcaggc cgtaacggtt gccgccgccg aatttgacgg aaatgttttc
    10621 aaacaacggc ttactgccga actgcatggt gacgttactg gaaactaaca caggcgtatc
    10681 ctgaaaagag atatgacaaa ccgcgcatta tgccataagt ctgaaagaag atcctgctcc
    10741 tcgcttaagg tctacactca agccaaaggc aaaaaatgtg atttcgtaca catctgattt
    10801 cactgtgagc tggaatgaac ttataatgcg cttccaatac tctaatattc tcaacccaat
    10861 ggcctgccag gcacaaaatc tcgcttaaca tgaatatgaa attgaaaaca ttattcgcag
    10921 cggccttcgc tgttgtcggc ttttgcagta ccgcctctgc ggtaacttat cctctgccaa
    10981 ccgacgggag tcgcctggtt ggtcagaatc aggtgatcac cattcctgaa ggtaacactc
    11041 agccgctgga gtattttgcc gcggagtacc agatggggct ttccaatatg atggaagcga
    11101 acccgggtgt ggataccttc ctgccgaaag gcggtactgt actgaacatt ccgcagcagc
    11161 tgatcctgcc ggataccgtt catgaaggca tcgtcattaa cagtgcagag atgcgtctgt
    11221 attactatcc gaaagggacc aacaccgtta tcgtgctgcc gatcggcatt ggtcagttag
    11281 gcaaagatac gcctatcaac tggaccacca aagttgagca taagaaagca ggcccgacct
    11341 ggacgccgac cgccaaaatg cacgcagagt accgcgctgc gggcgaaccg ctaccggctg
    11401 tcgttccggc aggtccggat aacccgatgg gtctgtatgc actctacatc ggtcgcctgt
    11461 atgctatcca tggcaccaac gccaacttcg gtatcggcct gcgtgtaagt catggttgtg
    11521 tgcgtctgcg taacgaagac atcaaattcc tgttcgagaa agtaccggtc ggtacccgcg
    11581 tacagtttat tgatgagccg gtaaaagcga ccaccgagcc agacggcagc cgttatattg
    11641 aagtccataa cccgctgtct accaccgaag cccagtttga aggtcaggaa atcgtgccaa
    11701 ttaccctgac gaagagcgtg cagacagtga ccggtcagcc agatgttgac caggttgttc
    11761 ttgatgaagc gatcaaaaat cgttccggga tgccggttcg cctgaattaa tcgtcaaaac
    11821 gtaaaacaaa ggcggactca taatccgcct tttttatttg ccagacctta ccaggccggg
    11881 atcataacta acaaaacgta tcccaccagc gccgcccaca acagcatcgg tattgaatag
    11941 agatggaaac gccaccagat gcggcgatcg ttcgccatac gtagcgcaat caaatttgcc
    12001 agcgatcctg gtaataaacc aaagcccccc acgtttaccg cccatgccag taataaagac
    12061 ggaggtacat aattgagcag caaaatggta ctcggcacgt tactgatcac ctgcgataaa
    12121 ccgactgccg ttaaccataa cccgggttca gataaatgac tcacgttacc caacacgcct
    12181 tgcaacgctg gaagctgggt cagtaaatgg acgtcgataa acatcgccat aaacaccagc
    12241 agcaacgtcc agtccacact caacaccacg cgacgcgcca gcagcacaaa gcccgccgcc
    12301 acaatcacca gtccccacag ctcttgtttg aactccagcg ccgtcagaaa gacgatatac
    12361 agccccagac aattccacac cagccgcggt ttccactccg gtgtttgcac ccccgtatgg
    12421 tattgcagcg cctttccagg gaaacaacac cagcacaaca gcaggagtgt cagcatcatt
    12481 gcgccagcca tcggtgccat ttgggcaata aatccggcaa acgaaagacc agaacgtccc
    12541 cagataagaa tattttgcgg gttgccaatt ggcgtcagta gcgaaccagc gttgactgcc
    12601 agcgcctcaa aaataatcag ccgattaacc gggatctcac acagtctttt tagcgtgata
    12661 gtcagcggaa caacaataaa cagcgcgaca tcgttggtca gaaaggtaga aagcagcgcc
    12721 gccgccagca ccataaacat cgccagccga cgctccgtag caaagcggcg caccattttg
    12781 cgccccagca catcaaaata accgcttaac tccacacctt tggtcagcag catcaggccg
    12841 cttaaggtga tgatggtgtg ccagtcgata gcagcaggcc aggatttcgg tgcaaagggc
    12901 acgaaaaagc ttaatccgat accaacaaga attaataact gaaaaaaacg atcgccttgc
    12961 agcgtgcgta aaaaaggcag gctcatttgg caccgtgttt ctgggtaaac aaacgaaaag
    13021 cgtcgagcgt ctcttcacta acatggtgct ccatgccttc cgcgtcgcga cgggcgattt
    13081 ccggactgac gcccaacacc aacaagaaat tttcgactat ctgatgtcgc tcgcggcttt
    13141 cctgcgccag cttctctcct tctgccgtta aaaacacgcc acgccagggg atcatttcaa
    13201 tcagccccat ggttgccagc cgcttaagca ttttagccac cgtcggttgc gaaactccca
    13261 gacgagcagc catgtccacc tgacgagctt cccccacttc cctgatcaag tcagaaatca
    13321 gctcaacgta atcatcaata agctcgcgcc gatgcgcctc tctgacctgg cggaaccctt
    13381 caacgtgctc ttccacgttc actaactgcg tcactttttt tgctattggc gtacctgcgc
    13441 gacgactcat tgtgcttcct cattatggtg acgcatacat agcgttctca tttcgtgcgc
    13501 acattgtaaa ccagagttgc gaaggtacaa aaaattaacg ttttagcaat agctatataa
    13561 tatagcctgt gctatatctg tatgtaatgc aatcatccct caaggatcga cgggattagc
    13621 aagtcaggag gtcttatgaa tgagttcaag aggtgtatgc gcgtgtttag tcattctccc
    13681 tttaaagtac ggttaatgct gctctctatg ttgtgcgata tggtcaacaa caaaccgcag
    13741 caagataaac cttcagataa atagcggcgt cgcggtacgc cgcttcactc ctgcttttat
    13801 gcaggcataa cgcgttttgg tctgaaaaaa accacttttt gtcggatttg caatcccctt
    13861 cgcaaaagat ttgttcgtca gtagttgacc tgaacggcgg ctcgctctat cttcttgcag
    13921 ccctgcgtat attgcggctc gcggatgcgg acccctttcc cctcttcacg cactcttgca
    13981 ggtattgacc cttgacgcca gggtaagcac atggcgtttg ttacgatagt ggcatatgaa
    14041 tttaaccctc aaagaatcgc ttgttacccg tagccgggta tttagcccgt ggactgcgtt
    14101 ctacttttta cagtcgctat taattaacct cggcttaggt taccccttca gtttgctcta
    14161 caccgctgcg tttacggcta ttttgctttt gctatggcga acattgcctc gcgtacaaaa
    14221 agttctggtc ggtgtcagtt cgctggtggc ggcttgttat ttcccttttg ctcaggccta
    14281 cggcgcgcct aatttcaata cattgctggc attgcactcc accaatatgg aagagtcgac
    14341 cgaaatcctg acgatttttc cgtggtacag ctacctggtc ggcttattta tttttgcgct
    14401 cggcgtaata gcaatcaggc gaaaaaaaga gaatgaaaaa gcgcgctgga ataccttcga
    14461 cagcctgtgt ctggtattca gtgtggcgac attttttgtt gctcccgtgc aaaacctggc
    14521 ctggggtggc gtatttaaac tgaaagatac tggctatccg gtatttcgtt ttgctaagga
    14581 tgtcatcgtc aataataacg aggtgattga agagcaagaa cggatggcaa aactttccgg
    14641 aatgaaagat acctggacgg tcactgccgt taagccgaag tatcagacct atgtggtagt
    14701 gatcggtgaa agcgcgcgtc gcgatgccct cggtgccttt ggcggtcact gggacaatac
    14761 cccgtttgcc agcagcgtta acggtttgat atttgctgac tacattgctg ccagtggctc
    14821 cacgcagaaa tcgcttggct taacgctcaa tcgcgtagtc gatggcaaac cacagtttca
    14881 ggataacttt gtcaccctgg caaatcgcgc gggcttccag acctggtggt tttccaacca
    14941 gggccaaatc ggcgaatacg atactgctat cgccagcatc gccaaacgtg cagatgaagt
    15001 gtacttcctg aaagaaggta attttgaagc agataaaaac acgaaagacg aagcgttact
    15061 ggatatgacc gctcaagtgc tggcgcaaga gcactcgcaa ccgcagctga ttgttctgca
    15121 tctgatgggc tcgcatccgc aggcctgcga caggacacaa ggcaaatacg aaacctttgt
    15181 gcaatcgaaa gaaacgtcgt gctatctcta taccatgacg caaacggacg atttactgcg
    15241 caagctgtac gatcagttac gcaacagcgg cagcagcttc tctctggttt acttttctga
    15301 ccacggtctg gcctttaaag agcgcggcaa agacgtgcaa taccttgccc atgatgataa
    15361 atatcagcaa aatttccagg tgccttttat ggtcatttcc agcggcgata aagcgcatcg
    15421 ggtgattaaa gcccgccgct cagccaatga cttcttaggc tttttctcgc agtggacggg
    15481 aattaaagcg aaggaaataa atatcaaata cccgtttata tctgagaaga aagccgggcc
    15541 gatatacatc accaacttcc agttacagaa ggtggattac aaccatctcg gaaccgatat
    15601 tttcgacccg aaaccttaaa aacaaaaatc cgccccgaga ggcggatttt tatatcacca
    15661 aagtgattag aagcggtaac caacaccggc aatccaggtg cctacgtcaa cgctacgaat
    15721 acggctctgc tcgtaagaga agtccagagc aacgttttcc atcgggttga actgcagacc
    15781 agcaccgtag gagaaaccgt agtcgctggt gtcgtgtttg taggtcgggt attcagtggt
    15841 ctggaattta ccataaccca cacccactac accgtagatg cttgcccagt cgttaatgcg
    15901 gtaagccgga ccagcagtga tgccgtagta ctggtttttg ttgtagtcac cagagcttgc
    15961 agtacggctt ttctcggtgt aagtgaaaga accgatcaca cccagcgggc tgttgtcttc
    16021 ttcatagcgg tatttcaggt tgaaaccgcc cattttgttc atttggccct gagcgtcgct
    16081 ctgtgcgtaa ccgccagtta cggtagaagt cgcagctacg gaagtacctg cggtgaaagc
    16141 cagaactgcg gccagtgctg aaagacatgc aatttttttc ataaccacct caaatgtgat
    16201 tcaaataagt cctaagtttt aaatatatca aaaattaatg ggaaactctt cgcgatttgt
    16261 gatgtctaac gggccatttc atgtaacaga acgtttccat acaccgctat ccatctaaat
    16321 ttaaatcact ttttcagaga actgcgtaag tattacgcat gttttccctg tcattcatcc
    16381 agattattcc taatcaccag actaatgatt ccatcaatcc tggcgcattt tagtcaaaac
    16441 gggggaaaat tttttcaaca aatgctcaac cagcattggg tatatccagt acactccacg
    16501 ctttacttaa gtctagatat ttgtgggaga aaggatgcct ggttcattac gtaaaatgcc
    16561 ggtctggtta ccaatagtca tattgctcgt tgccatggcg tctattcagg gtggagcctc
    16621 gttagctaag tcactttttc ctctggtgag cgcaccgggt gtcactgcgc tgcgtctggc
    16681 attaggaacg ctgatcctca tcgcgttctt taagccatgg cgactgcgct ttgccaaaga
    16741 gcaacggtta ccgctgttgt tttacggcgt ttcgctgggt gggatgaatt atctttttta
    16801 tctttctatt cagacagtac cgctgggtat tgcggtggcg ctggagttca ccggaccact
    16861 ggcggtggcg ctgttctctt ctcgtcgccc ggtagatttc gtctgggttg tgctggcggt
    16921 tcttggtctg tggttcctgc taccgctggg gcaagacgtt tcccatgtcg atttaaccgg
    16981 ctgtgcgctg gcactggggg ccggggcttg ttgggctatt tacattttaa gtgggcaacg
    17041 cgcaggagcg gaacatggcc ctgcgacggt agcaattggt tcgttgattg cagcgttaat
    17101 tttcgtgcca attggagcgc ttcaggctgg tgaagcactc tggcactggt cggttattcc
    17161 attgggtctg gctgtcgtta ttctctcgac cgctctgcct tattcgctgg aaatgattgc
    17221 cctcacccgt ttgccaacac ggacatttgg tacgctgatg agcatggaac cggcgctggc
    17281 tgccgtttcc gggatgattt tcctcggaga aacactgaca cccatacagc tgctggcgct
    17341 cggcgctatc atcgccgctt caatggggtc tacgctgaca gtacgcaaag agagcaaaat
    17401 aaaagaatta gacattaatt aaatttacat ttctgtatgg ttatgcataa ccatgcagaa
    17461 tttctcgcta cttttcctct acaccgtatt tatatatcga attatgcaaa agcatattta
    17521 ttccgaaaat tcctggcgag cagataaata agaattgttc ttatcaatat atctaactca
    17581 ttgaatcttt attagttttg tttttcatgc ttgttaccac tattagtgtg ataggaacag
    17641 ccagaatagc ggaacacata gccggtgcta tacttagtct cgttaattac tgggacataa
    17701 catcaagagg atatgaaatt atgagtaccg ctaaattagt taaatcaaaa gcgaccaatc
    17761 tgctttatac ccgcaacgat gtctccgaca tacccgcaac gatgtctccg acagcgagaa
    17821 aaaagcaaca gtagagttgc tgaatcgcca ggttatccag tttattgatc tttctttgat
    17881 taccaaacaa gcgcactgga acatgcgcgg cgctaacttc attgccgtac atgaaatgct
    17941 ggatggcttc cgcaccgcac tgatcgatca tctggatacc atggcagaac gtgccgtgca
    18001 gctgggcggt gtagctctgg ggaccactca agttatcaac agcaaaaccc cgctgaaaag
    18061 ttacccgctg gacatccaca acgttcagga tcacctgaaa gaactggctg accgttacgc
    18121 aatcgtcgct aatgacgtac gcaaagcgat tggcgaagcg aaagatgacg acaccgcaga
    18181 tatcctgacc gccgcgtctc gcgacctgga taaattcctg tggtttatcg agtctaacat
    18241 cgaataaatc catcgctgat ggtgcagaac tttagtaccc gataaaagcg gcttcctgac
    18301 aggaggccgt tttgttttgc agcccacctc aacgcactta tttagtgcat ccatctgcta
    18361 tccccagctg attaagtaaa ttttttgtat ccacatcatc acacaatcgt tacataaaga
    18421 ttgttttttc atcaggtttt acgctaaata atcactgtgt tgagtgcaca attttagcgc
    18481 accagattgg tgccccagaa tggtgcatct tcagggtatt gccctataaa tcgtgcatca
    18541 cgtttttgcc gcatctcgaa aaatcaatga gttgcaaaat tggcacgatt ttttcatata
    18601 tgtgaatgtc acgcagggga tcgtcccgtg gatagaaaaa aggaaatgct atgaagtctg
    18661 tattaaaagt ttcactggct gcactgaccc tggcttttgc ggtttcttct catgccgcgg
    18721 ataaaaaatt agttgtcgcg acggataccg ccttcgttcc gtttgaattt aaacagggcg
    18781 ataaatatgt gggctttgac gttgatctgt gggctgccat cgctaaagag ctgaagctgg
    18841 attacgaact gaagccgatg gatttcagtg ggatcattcc ggcactgcaa accaaaaacg
    18901 tcgatctggc gctggcgggc attaccatca ccgacgagcg taaaaaagcg atcgatttct
    18961 ctgacggcta ctacaaaagc ggcctgttag tgatggtgaa agctaacaat aacgatgtga
    19021 aaagcgtgaa agatctcgac gggaaagtgg ttgctgtgaa gagcggtact ggctccgttg
    19081 attacgcgaa agcaaacatc aaaactaaag atctgcgtca gttcccgaac atcgataacg
    19141 cctatatgga actgggcacc aaccgcgcag acgccgttct gcacgatacg ccaaacattc
    19201 tgtacttcat caaaaccgcc ggtaacggtc agttcaaagc ggtaggtgac tctctggaag
    19261 cgcagcaata cggtattgcg ttcccgaaag gtagcgacga gctgcgtgac aaagtcaacg
    19321 gcgcgttgaa aaccctgcgc gagaacggaa cttacaacga aatctacaaa aaatggttca
    19381 gtactgaacc gaaataataa cgctacacct gtaaagcgca ctggcagttc cctctccccc
    19441 ctggggagag gattagggtg aggggcgcaa acccgctccg gggccattaa ttaccctgaa
    19501 tttgattatt tacaccacgg taacaggaac aacatatgca gtttgactgg agtgccatct
    19561 ggcctgccat tccgcttctg attgaaggtg ccaaaatgac cctgtggatt tcggtcctcg
    19621 gtctggctgg cggtctggta atcggattgc tggcaggttt tgcacgcacc ttcggtggtt
    19681 ggatagccaa ccacgtcgcg ctggtcttta ttgaagtgat ccgcggcaca cctatcgtcg
    19741 tccaggtgat gtttatctat ttcgccctgc cgatggcgtt taacgactta cgcatcgacc
    19801 catttactgc ggcggtggtc accatcatga tcaactccgg cgcgtacatt gcggaaatca
    19861 cgcgtggtgc ggtgctgtct atccacaaag gttttcgtga agcaggactg gcgctcggtc
    19921 tttcacgttg ggaaaccatt cgctacgtca ttttaccgct ggcactgcgt cgtatgctgc
    19981 cgccgctggg taaccagtgg atcatcagca ttaaagacac ctcgctgttt attgttatcg
    20041 gcgtggcgga actgacccgt caggggcaag aaattattgc cggtaacttc cgcgcccttg
    20101 agatctggag cgccgtggcg gtgttctatc tgattattac cctggtgctg agctttattc
    20161 tgcgtcgtct ggaaagaagg atgaaaatcc tgtgattgaa tttaaaaacg tctccaagca
    20221 ctttggccca acccaggtgc tgcacaatat cgatttgaac attgcccagg gcgaagtcgt
    20281 ggtgattatc gggccgtccg gttccggtaa atcgaccctg ctgcgctgca tcaacaaact
    20341 ggaagaaatc acctccggcg atctgattgt cgatggcctg aaggtcaacg atccgaaagt
    20401 tgacgagcgc ctgattcgcc aggaagcagg tatggtgttc cagcagtttt acctcttccc
    20461 gcatctgacg gcgctgggaa acgtcatgtt tggtccgcta cgcgtgcgtg gcgcgaacaa
    20521 agaagaggcg gaaaaactgg cgcgtgagct gctggcgaaa gtcggtctgg cagaacgtgc
    20581 acatcactac ccttccgaac tttctggtgg tcaacagcag cgtgtggcga ttgcccgcgc
    20641 gctggcggtg aagccgaaaa tgatgctgtt tgatgaaccg acttccgctc ttgacccgga
    20701 actgcgccat gaagtgctga aggttatgca ggatctggct gaagaaggga tgacgatggt
    20761 gatcgtgacc cacgaaatcg gttttgccga gaaagtagct tcgcggctga tctttatcga
    20821 caaaggccgg attgcggaag atggcaatcc gcaggtgttg atcaagaacc cgccgagcca
    20881 gcgcttgcag gaatttttgc agcacgtctc ttaataagac acattgcctg atgcgctacg
    20941 cttatcaggc ctacaggata tctggcaact tattaaaatt gcatgaactt gtaggacgga
    21001 taaggcgttc acgccgcatc cggcaaaaaa gcccgcacgt tgtcagcaac ctgcttaata
    21061 tcccttcctc cctttcaccc gaaagggagg cacaccagat tcctctcatt taaaatcgcc
    21121 cctcccccag catctatact tatctttttg ctctgttttc tcactggagg agtcatgcgg
    21181 tggatcctgt tcatcctctt ctgcctgctg ggcgcacctg cccacgcggt atccataccc
    21241 ggcgttacaa ccacaacgac aacggactca acgactgaac cggccccgga accggatatc
    21301 gaacaaaaaa agcggcctat ggcgcactgg cggatgtgct ggataacgac acctcgcgta
    21361 aagagttgat cgaccagttg cgtactgtcg ccgcgacgcc ccctgcggaa ccggtcccga
    21421 agatcgtgcc gccgacgctg gttgaagagc aaaccgtgct gcaaaaggtc accgaagtca
    21481 gccgccatta tggtgaagcc ctttccgccc gcttcgggca actttatcgc aatatcaccg
    21541 gctccccgca taagccattt aatccacaaa ccttcagcaa tgcgctgagc catttttcaa
    21601 tgttagcggt attagtgttt ggtttttact ggctgattcg cctgtgcgca ctgccgctat
    21661 atcgcaaaat gggtcagtgg gcgaggcaaa aaaatcgtga gcgcagcaac tggttgcagc
    21721 ttccggcgat gattattggg gcgtttatta tcgacctgct gttactggcc ctgacattat
    21781 ttgtcggcca ggtattaagc gacaacctga atgcaggcag tcgcaccatc gctttccaac
    21841 aaagtttgtt tctcaacgcc tttgccctga ttgaattttt caaagccgta ctacgcctga
    21901 ttttttgccc aaacgtggcg gagctgcgcc cgttcacgat tcaggacgag agcgcccgtt
    21961 actggggtcg tcgcctgagc tggttaagca gcctgattgg ctatggcctg attgtggccg
    22021 tgccgattat ctctaatcag gtgaatgtac agataggtgc gctggcgaac gtcatcatta
    22081 tgctgtgcat gaccgtctgg gcgttgtacc tgatcttccg aaataaaaaa gagattaccc
    22141 agcatatgct caactttgcg gagcattcgc tggctttttt cagcctgttt atccgcgcct
    22201 ttgcgctggt gtggcactgg ctggcaagcg cctattttat catgctgttt ttcttttcgt
    22261 tgttcgatcc gggcaacagt ctgaaattta tgatgggtgc aacggtgcgc agcctggcga
    22321 ttattggtat cgcagcgttt gtttccggta tgttttcccg ctggctggcg aaaaccatca
    22381 ccctctcgcc acatactcag cgtaactatc cggagctaca aaaacggctg aatggctggc
    22441 tttcggcggc gctgaaaacg gcgcgtattc tgaccgtctg cgtggcagta atgttgctgt
    22501 tgagcgcatg gggattgttc gatttctgga actggctgca aaacggcgcg gggcagaaaa
    22561 ccgtagatat cctgatccgc atcgcactca ttcttttctt ctcggcggtt ggctggacgg
    22621 tgctcgccag tttgatcgaa aaccggctgg cttcggatat tcatggccgc ccgctaccca
    22681 gcgcccgtac gcgtaccctg ctgacgctgt tccgtaacgc gctggcggtg attatcagta
    22741 ccatcaccat catgattgtg ttgtcggaaa tcggcgtcaa tatcgcgcca ttgctggcag
    22801 gtgccggggc attaggtctg gctatctcgt ttggttcgca aacgctggta aaagatatta
    22861 tcaccggggt atttattcag tttgaaaacg gcatgaacac tggagatttg gtgactatcg
    22921 ggccgttgac cggcacagtg gaacggatgt cgattcgctc cgtgggcgtg cgccaggata
    22981 ccggggcgta tcacatcatt ccgtggtctt cgataaccac ctttgctaac ttcgtccgcg
    23041 gtattggttc ggtagtggca aactacgatg ttgatcgcca tgaagatgct gataaagcca
    23101 atcaggcact gaaagatgcg gtaacggaat tgatggaaaa cgaagaaatt cgcgggctga
    23161 ttattggtga accgaacttt gccgggattg tcggcttaag caataccgcg tttacactgc
    23221 gtgtttcgtt caccacgctg ccactcaaac agtggacggt acgctttgcc ctcgacagcc
    23281 aggtgaaaaa acatttcgac ctggcgggcg ttcgcgcgcc agtgcagact tatcaggtgc
    23341 tgcctgctcc gggcgcgacc ccggctgaac cgctgccgcc gggggagcca acgctttaac
    23401 gttggcgatt aacaaaacgg cggcgctgct cgtcgttcat aaaggtccag gcaataaagc
    23461 gactctgctt ttgcccctgg gccatctctt tttttaccac cttcaccgcg cccacgtcgg
    23521 tcagagcacg atacaacggc ggtaagtttt caccacgaga taccagcgag gtaaaccaca
    23581 tcacctgctt cgcgaagcct ttgctctctt caatcatctt tttgataaag ttgacttcac
    23641 cgccttcaca ccacaactcc tgttgctgac caccaaagtt cagtgcatcg tctttgttca
    23701 gccccaggtt acggcgttta cgctcactac ctgcccgcgc cgcagcggcg gaatcgtgga
    23761 atggcgggtt acacaaggtc gcgtcgtatt gctcgttttt gtggatgatg ccgttaaaaa
    23821 tcgccccact ctctttttgc cgacgcagac gaatggcgcg gttaagcccc ggattagcac
    23881 tgataatcgc ctgcgcactg cttaacgcct ggctgctggt ttcactaccg gtaaaacgcc
    23941 agccatattc atgtacgcca atcagcggat aaatacagtt cgcaccaacg ccgatatcca
    24001 gaatgctggc attcgctgga attgttccgc ttgcttcagc cagtaaatcg gcaaggtgat
    24061 gaatataatc cgcccggccc ggtactggtg ggcagagaaa accgtcgggg atatcccagt
    24121 tcgctacggc gtaaaaatgg gccagcaacg ccttattgag cgccttcacc gccagcggat
    24181 tggcaaagtc tacgctttgc tcccctgcgg gtgtaagcgt gaggaattgc ctgagttcag
    24241 gattgacctg acaaagcgtg gcgagatcgt agcggctgtg atgacggttg cgcggatgca
    24301 accccggttt ctgggcggac atagcattct cctttaagca gcggcgtaag atacccgcat
    24361 aacgcgacag ggtaaatatc tggctgcgtt tatcattccc caaaacctta acatggctat
    24421 ggtttatggc aattcatcat aaaaatcagc attatgcatg tcattgattt gtcttatttt
    24481 gtaaaaatat atcgttcaaa aaataagcat ttctctgtca attttttgcg tcatttgcat
    24541 cgcgcaggcg acctacactt atcaggcact acccgttgta tttatgagga tgtcattatg
    24601 aaaaagtgcc tcacactact gattgccacc gtcctgagcg gaatctctct cacggcttat
    24661 gccgcgcaac cgatgagtaa cctggacagc ggtcagctac ggcccgccgg taccgtttcg
    24721 gcaactggcg catcaaacct aagcgatctg gaggataaac tggcagaaaa agcgcgcgaa
    24781 caaggcgcga aaggttatgt cattaattcc gcaggcggaa atgaccagat gttaggtact
    24841 gcaaccatct acaaataacc ccaccaacgt aactgcattc gcctctggat gcagttttcg
    24901 cctgttttgt aatcaaaaaa catcaaatat gaacgcaatg taaataaatg tatttctttt
    24961 tcgcgcaatg ggtgatagaa aatcgcatca agtgataatg cttatcaaaa ttattatcac
    25021 ttgcacgaac actatcacgg gattaacagt ggcatcgcat ccgcagagat gctttctcgt
    25081 ggcagtgaaa atttcaacat ataagaaaaa gtcacctgca aaatggaaaa caatcgcaat
    25141 ttccctgcca gacaatttca ttcgctcacg ttctttgccg gtctttgtat tggtattacg
    25201 cctgtggcac aggcactcgc cgccgaaggg caagctaacg cggatgacac gctggttgtc
    25261 gaagcatcaa cgccttcgct ttatgcgcca caacaatctg ccgatccgaa attctcgcgt
    25321 ccggtagcgg atactacccg cacgatgacg gtgatttctg aacaagtgat taaagatcag
    25381 ggcgcaacca accttaccga cgcgctcaaa aacgtccccg gcg
//
LOCUS       DAZSNM010000036        25348 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZSNM010000036 DAZSNM010000000
VERSION     DAZSNM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25348
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.036"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1195
                     /gene="helD"
                     /locus_tag="ACHW42_001268"
     CDS             <1..1195
                     /gene="helD"
                     /locus_tag="ACHW42_001268"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR8943164.1"
                     /translation="KKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTE
                     EMQWSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRI
                     KLMAPLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRA
                     ALLAALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFN
                     SRIGEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERI
                     LILARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAA
                     RESIMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVP
                     VARKP"
     gene            complement(1227..1685)
                     /gene="mgsA"
                     /locus_tag="ACHW42_001269"
     CDS             complement(1227..1685)
                     /gene="mgsA"
                     /locus_tag="ACHW42_001269"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR8943165.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(1781..2443)
                     /locus_tag="ACHW42_001270"
     CDS             complement(1781..2443)
                     /locus_tag="ACHW42_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR8943166.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            2616..3029
                     /locus_tag="ACHW42_001271"
     CDS             2616..3029
                     /locus_tag="ACHW42_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR8943167.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(3074..3391)
                     /gene="hspQ"
                     /locus_tag="ACHW42_001272"
     CDS             complement(3074..3391)
                     /gene="hspQ"
                     /locus_tag="ACHW42_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR8943168.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(3449..4639)
                     /gene="rlmI"
                     /locus_tag="ACHW42_001273"
     CDS             complement(3449..4639)
                     /gene="rlmI"
                     /locus_tag="ACHW42_001273"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFR8943169.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            4734..5012
                     /gene="yccX"
                     /locus_tag="ACHW42_001274"
     CDS             4734..5012
                     /gene="yccX"
                     /locus_tag="ACHW42_001274"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR8943170.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(5009..5338)
                     /gene="tusE"
                     /locus_tag="ACHW42_001275"
     CDS             complement(5009..5338)
                     /gene="tusE"
                     /locus_tag="ACHW42_001275"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR8943171.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(5429..6088)
                     /gene="yccA"
                     /locus_tag="ACHW42_001276"
     CDS             complement(5429..6088)
                     /gene="yccA"
                     /locus_tag="ACHW42_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR8943172.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(6295..6382)
                     /locus_tag="ACHW42_001277"
     tRNA            complement(6295..6382)
                     /locus_tag="ACHW42_001277"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6346..6348),aa:Ser,seq:tga)
     gene            6808..7926
                     /gene="hyaA"
                     /locus_tag="ACHW42_001278"
     CDS             6808..7926
                     /gene="hyaA"
                     /locus_tag="ACHW42_001278"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR8943173.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            7923..9716
                     /gene="hyaB"
                     /locus_tag="ACHW42_001279"
     CDS             7923..9716
                     /gene="hyaB"
                     /locus_tag="ACHW42_001279"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR8943174.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            9735..10442
                     /gene="hyaC"
                     /locus_tag="ACHW42_001280"
     CDS             9735..10442
                     /gene="hyaC"
                     /locus_tag="ACHW42_001280"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFR8943175.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            10439..11026
                     /gene="hyaD"
                     /locus_tag="ACHW42_001281"
     CDS             10439..11026
                     /gene="hyaD"
                     /locus_tag="ACHW42_001281"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR8943176.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            11023..11421
                     /gene="hyaE"
                     /locus_tag="ACHW42_001282"
     CDS             11023..11421
                     /gene="hyaE"
                     /locus_tag="ACHW42_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR8943177.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            11418..12275
                     /locus_tag="ACHW42_001283"
     CDS             11418..12275
                     /locus_tag="ACHW42_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR8943178.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            12409..13953
                     /gene="appC"
                     /locus_tag="ACHW42_001284"
     CDS             12409..13953
                     /gene="appC"
                     /locus_tag="ACHW42_001284"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR8943179.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            13965..15101
                     /gene="appB"
                     /locus_tag="ACHW42_001285"
     CDS             13965..15101
                     /gene="appB"
                     /locus_tag="ACHW42_001285"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR8943180.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            15113..15205
                     /gene="cbdX"
                     /locus_tag="ACHW42_001286"
     CDS             15113..15205
                     /gene="cbdX"
                     /locus_tag="ACHW42_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR8943181.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            15285..16583
                     /gene="appA"
                     /locus_tag="ACHW42_001287"
     CDS             15285..16583
                     /gene="appA"
                     /locus_tag="ACHW42_001287"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR8943182.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(16698..18864)
                     /gene="etk"
                     /locus_tag="ACHW42_001288"
                     /pseudo
     CDS             complement(16698..18864)
                     /gene="etk"
                     /locus_tag="ACHW42_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(18884..19330)
                     /gene="etp"
                     /locus_tag="ACHW42_001289"
                     /gene_synonym="yccY"
     CDS             complement(18884..19330)
                     /gene="etp"
                     /locus_tag="ACHW42_001289"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR8943183.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(19318..20457)
                     /locus_tag="ACHW42_001290"
     CDS             complement(19318..20457)
                     /locus_tag="ACHW42_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR8943184.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(20503..22599)
                     /locus_tag="ACHW42_001291"
     CDS             complement(20503..22599)
                     /locus_tag="ACHW42_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR8943185.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(22599..23345)
                     /locus_tag="ACHW42_001292"
     CDS             complement(22599..23345)
                     /locus_tag="ACHW42_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR8943186.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(23342..23986)
                     /gene="gfcB"
                     /locus_tag="ACHW42_001293"
     CDS             complement(23342..23986)
                     /gene="gfcB"
                     /locus_tag="ACHW42_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR8943187.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(24093..24398)
                     /locus_tag="ACHW42_001294"
     CDS             complement(24093..24398)
                     /locus_tag="ACHW42_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8943188.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(24840..25052)
                     /gene="cspH"
                     /locus_tag="ACHW42_001295"
     CDS             complement(24840..25052)
                     /gene="cspH"
                     /locus_tag="ACHW42_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR8943189.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         6066 a         6427 c         6650 g         6205 t
ORIGIN      
        1 caaaaaagtt ccgatagtca gcaaactgga aaatgatacc gctgcccgtc atgaactctt
       61 tattgctgag tggcgcaagc aatgcagcga aaagaaagcg caggcgaagg gctggcggca
      121 atggctgacg gaagaaatgc agtggtcagt gccagaaggt aacttctggg atgatgaaaa
      181 attacagcgt cgccttgcct ctcgcctcga tcgctgggta agtctgatgc ggatgcacgg
      241 tggtgcacag gcagaaatga ttgccagtgc acccgaagag attcgcgatc tgttcagtaa
      301 acgtatcaag ttgatggccc cgttattaaa agcctggaaa ggtgcgctga aggcagaaaa
      361 cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg attctggaga aaggccgctt
      421 tatcagcccg tggaagcata ttctggttga tgaatttcag gatatctcgc cgcagcgcgc
      481 agcgttgtta gcggcattac gcaagcaaaa cagtcagacg acgttgttcg ccgttggtga
      541 tgactggcag gcgatttacc gattcagcgg tgcgcaaatg tcgctcacca ccgctttcca
      601 tgaaaacttt ggtgaaggcg atcgctgtga tttagacacg acttaccgtt ttaacagtcg
      661 tatcggtgag gtggcaaacc ggtttattca gcagaaccca ggccagctga aaaagccgct
      721 aaacagctta accaatggag acaaaaaagc cgtcacgtta ttggatgaga gtcaacttga
      781 cgctttgctg gataagctct ctggttatgc caaaccggaa gagcgcattc tgatcctggc
      841 gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg gcaacacgct ggccgaagtt
      901 gcaaatcgac tttatgacca ttcatgccag caaagggcaa caggcggatt acgtcatcat
      961 cgttggcttg caggagggga gtggtggttt tccggctgcg gcgcgggagt cgattatgga
     1021 agaggcgcta ctgccaccgg ttgaggattt cccggacgct gaagaacggc gtttaatgta
     1081 cgtggcgctg acccgggcac gccatcgggt atgggcactg tttaacaaag agaatccctc
     1141 tccctttgtg gaaatactga aaaatctgga tgtgccggtg gcgagaaaac cgtaagaaac
     1201 aggtggcgtt tgccacctgt gcaatattac ttcagacggt cagcgagata acgctgataa
     1261 tcggggataa gaatatcgac cgcgtcgttg aaatgcggcg actggataat gaagtctgcc
     1321 gttgccacgt tggtggcgac cggaatgttc cataccgtcg ccagacgcag caaggctttc
     1381 acgtcaggat cgtgcggcac ggcatttagt ggatcccaga agaatatcaa tacatcaatt
     1441 ttcccttctg agatcaatgc gccaacctgc tggtcacccc ccattgggcc actcaacatc
     1501 gcgttgacgt tcatgccggt cgcgcgggaa atcaagttac cggtagtgcc tgttgcatac
     1561 agtacgtgtt gttccagtaa cggttgatgc cgttccaccc agctcatcag catttgtttg
     1621 cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag gtaaagtgcg agtcgtcagt
     1681 tccataatgt acatccgtag ttaactttcc tacagattac tgtaagcact tatcgctgca
     1741 agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc tcaggaagat ggttgttttt
     1801 ccgcccattg caggaaagta tttcgcgttt gtgggtcagc cagtttaaac caatacttca
     1861 gccgttgttc tgtgagcacc tgagactgcg gtggaatagc atccagctcg gaaacacccg
     1921 aaagcagcgt actgtcatct gccatcatcg tggcaaattg cggtagtgaa gcgcgtttcg
     1981 cggatttgtt atagcgttca acctcgacct cataatcaat cgtttttgcc gttgaggtaa
     2041 tggcgagaat atccagcttt accggaatcg gcgtcgcatc gccatccaac aattcaaggc
     2101 gcggcgcggc atcaaaatgg ttagcttccc gctcattctc caggcgaggc aggcgaaaat
     2161 tgacctggtt gatgagctgg gtattaaaac tgaccaccag cggtggggag atatacagcc
     2221 gttcttcact attggaaaga tgaatcgtct tctcaacgcg aaacactaac tgatgcggtc
     2281 cattgtccag ttcaatgctg tccgcaccgc gcagcagaga gctggaaacc ttcttgccgt
     2341 cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt ggtggcaaaa acggataccg
     2401 gcaaacataa tgcaatcaag gtggtcacga tgccggtttt cataggtagc tcctgtcaaa
     2461 agaccgcccg aataataatg tgtcaaaatt tttctttaat catacttact aacatataga
     2521 catatttccc gcttttgctc tcattcattc gtattagctg catggttggc atgtcggctt
     2581 tgtcgtacac tttgttaaac agtctggagg aaataatgaa agaaaccgat attgcaggca
     2641 ttttaacgtc cactcacacc attgcgctgg tgggggcaag cgacaaaccc gatcgcccca
     2701 gctatcgcgt gatgaaatat ctactcgacc aggggtatca cgtcattccg gtttcgccaa
     2761 aagttgccgg caaaacgctg ctgggccagc aggggtatgg cacgctggca gacgttccgg
     2821 aaaaggtcga tatggtcgat gttttccgta attcagaagc ggcgtggggc gtggcacagg
     2881 aagctattgc cattggcgcg aaaaccctct ggatgcaact gggggtgatt aatgagcaag
     2941 cggcagtact ggcacgggat gccgggttaa atgtggtgat ggatcgctgc ccggctattg
     3001 agatccctcg cctgggtctg gccaaataaa aaatccccgg aaggcaaaaa ccttccgggg
     3061 atttgttcag ggattagtta cgcagacgcg gggcctggag ttgtttgcgg atggtctgcg
     3121 ccagctcatc catggatggc tgttcaggat gttcatcctg caattcgcta cttagctggg
     3181 cttcagccag gtaggtatga accggtaggc cgttatcgtc ctccatcacc acgtgatacc
     3241 acggagcggc gcgaagctcg tcattcaccg ccagctcatc gggcgacggt tcagagagcg
     3301 aataaaccgg gtcgatatcc acgaccactc cgagataacc taacagggaa tggcggacct
     3361 gctggccgat accgaatttg ctggcaatca tagtcacctc ccgggaaatc tttacctttg
     3421 atataagggt aaaattccac atttcaagtt acatgacgcg acaggcaaac cctttcagat
     3481 atagcccttc cgggtaggta gcgatcaccg gatgatcggc tgcctgacgg aactgctcta
     3541 taaattgtac atcacgaccg gcatcaattg cggcatccgc gatgattttc tgaaataaat
     3601 cgctggtcat cagaccggaa caggagaaag tcaggagaat accgccttca ttcagcaact
     3661 gaatcgccag catgttgatg tctttataac cacgacacgc gcccatcaac tggcttttat
     3721 tctcaacaaa cttcggcggg tccatcacga taacgtcaaa tttttcaccg cgatcgcgat
     3781 aagtacgcag caatttaaag acatcatcac ggacaaactc agccttgctc agatccagtt
     3841 tgttcagctc aacgttctgc cgtgcaatat ccagcgcttc ctgggaggta tcaacgctga
     3901 caacctggct gcaaccgccc atcagtgccg atacggcgaa accaccggta taggagaaac
     3961 agttcagcac acgtttattt tcaacgtagc ggcgggtagc caggcggctg tctcgctggt
     4021 ccagatagta gcccgttttg tgtccgtgct gaatatccac cagcagcttc atgccgtgtt
     4081 cttcaatcgg cagcagggca ggcggcaact cgccggtgac gaggccctgg gtcagctcca
     4141 tcccttcttt tttacgtacc gcaacatcgc tgcgatcgta aatcgcacat tccgggtaca
     4201 gcgtttgcag ggcactaatt aatgccgcgc gctgatattc tgcgccagca ctcagcagtt
     4261 gcagcaccag aaaattaccg aaacgatcga tagtaatacc cggcaggcca tcagattctc
     4321 cggcgattaa acgatagctg tcgaggccat ctttttgcgc cagccagtca cgccattttt
     4381 gtgcttgttg caaacggcgg gaaaaaaaag caatgtcgat agactcagac gggtcaaacg
     4441 tccagacgcg cgcccggatt tgcgaagctg gcgaataagc gccgcgtgct aaccattttc
     4501 cctgatgatc aacaatatcg atggtttcac cgaggctggc tttaccttcc atgcgggcaa
     4561 cggccccgga aaagacccac ggatggcgac gaagtaatga tttttcgcgc cctttggcta
     4621 acactaaacg tacactcata atttactttt ctgtcgatgc aaagaaatgg gcgtcattgt
     4681 ccggagtttt ccatggattt gcaacgcgtc cattaaggat aaggagagca gcaatgtcga
     4741 aaatctgcat aattgcctgg atttacgggc gagttcaggg cgtaggattt cgctacacca
     4801 cacagtacga agcgaaaaga ctgggcttaa ccgggtacgc caaaaatctt gacgatggca
     4861 gcgtggaagt ggttgcctgc ggtgaagaag ggcaggtaga aaaattaatg cagtggttaa
     4921 agagtggcgg cccgcgttct gcgcgtgtag aacgggtgct cagcgagccg catcatccct
     4981 cgggggaatt aaccgatttt cgtatccgtt aaatacattt taccggtttt ggcaggccag
     5041 caattttggt ggcttgctta gccgggcctt tcgggaacag tcggtataaa tagcggctgt
     5101 tacctttttc ttcaccaaat ttattcgcca tcgcttttac cagcatacga atcgccggag
     5161 acgtattaaa ttccaggtag aagtcacgta caaaacgcac cacttcccag tgttctggcg
     5221 acagcgaaat cccttcgttc tctgcaatca ccactgccag cggttcactc cactggctgc
     5281 tttctttgag atagccttcg gtatccgttt ctatctcttt accttcgaag atcagcataa
     5341 ttattactac cttaatcaga ctggcggcag tgtaacaaaa aacaaagccc cgcataaagc
     5401 gaggctatga aagtgttagc gggtgagatt aatcgcggct ggcgaagccc agaatgctca
     5461 gtaggctgac gaagatgttg tacagcgaaa catacaggct aaccgtggca cgaatatagt
     5521 tcgtttcacc gccatgaatg atgttgctgg tttcaaacaa gatagcgcca gaggagatca
     5581 gaatgaagac cgcgctgatc gccagatgca gagcaggcag ttgcaggaag atattagcaa
     5641 tcataccaat cagtaccacc acaatacccg ccatcagcat accgccgagg aacgacatat
     5701 ctttgcgggt ggtcagcaca tatgcagagc agcagaagaa cactaacgcc gttccgccca
     5761 gtgccatagc gattacgtca cccattccgg cagacagata ggtgttcaga attggtccga
     5821 ggatataacc cagaaaaccg gtaaaggcga atgcggagat aatcccggtc ggcttattcg
     5881 ccgttttata ggtcaggaac atcaaaccat acatacccac cagagtcaga atcagacccg
     5941 gagatggcag catcagcacc gtactggcag ttgcggtaat cgccgaaaag gccagcgtca
     6001 ggctcagcag aaaataggta ttacgcagca ccttatgggt gctaagcagt gatgtacggt
     6061 catgtgaaga actaacaata cgatccattg agtcactctc tatgacagat gtaattaatt
     6121 aagcagcata atgataatgc gtaagggcac ccagaagttt tacccatctt tacgcatttg
     6181 atctggaaca ggtttaacag cggattatca ggtcattaag caaatataac gccctgagaa
     6241 ttccgacagg caaaagaaaa agggggtagc gtttagctaa ccccttatct tatttggcgg
     6301 aagcgcagag attcgaactc tggaaccctt tcgggtcgcc ggttttcaag accggtgcct
     6361 tcaaccgctc ggccacactt ccggaatgac gcgcactata aacatcccga tgcggcgtgt
     6421 aaacccctaa tttgtttgtt tgcctgaaaa acagccaaaa gtgcattgat agcgtgaaat
     6481 aacagcagat tgatcatttc atcaccatga attccttctc ttttactcgt ttagcaaccg
     6541 gctaaacatc cccatcgccc ggctaaagaa aaataggtcc atttttatcg ctaaaagata
     6601 aatccacaca gtttgtattg ttttgtgcaa aagtttcact acgctttatt aacaatactt
     6661 tctggcgacg tgtgccagtg cagaaggatg agctttcgtt ttcagcatct cacgtgaagc
     6721 gatggtttgc cttgctacag ggacgtcgct tgccgaccat aagcgcccgg tgtcctgccg
     6781 gtgttgctag gagaagagac gtgcgatatg aataacgagg aaacatttta ccaggccatg
     6841 cggcgtcagg gcgttacccg gcgcagcttt ctcaaatatt gtagtctggc tgccacgtcg
     6901 ctgggattag gcgcgggaat ggcaccaaag attgcctggg cgctggagaa caaaccgcgc
     6961 attccggtgg tatggatcca cggtctggaa tgtacctgct gtaccgaatc ttttatccgc
     7021 tccgctcacc cactggcgaa ggacgtcatc ctttccctga tttccctcga ttacgacgat
     7081 accctgatgg ctgccgccgg aacccaggcg gaagaagtct ttgaagacat catcacgcaa
     7141 tataatggca aatatatcct cgcagtagaa ggtaatccgc cgctgggcga gcaggggatg
     7201 ttctgtatca gcagcggtcg accgtttatt gagaaactca aacgtgccgc tgccggagcc
     7261 agcgcgatta tcgcctgggg aacctgcgcg tcctggggct gcgtgcaggc cgcgcgacct
     7321 aatccgacac aggcaacgcc tatcgacaaa gtcatcaccg acaaacccat tatcaaagta
     7381 cctggctgcc cgccgatccc ggatgtgatg agcgccatca ttacttacat ggtgaccttt
     7441 gatcgcttgc cagatgtcga cagaatgggt cgtccactga tgttttatgg tcagcgtatc
     7501 cacgataaat gctatcgccg cgctcacttc gacgccggag agttcgtcca gagttgggat
     7561 gatgacgctg cccgcaaagg ttattgcctg tacaaaatgg gctgcaaagg gcctaccacc
     7621 tataacgcct gttcctccac acgctggaat gatggcgttt ctttcccaat ccagtctggt
     7681 cacggctgcc tgggctgtgc ggaaaatggt ttctgggatc gcggttcgtt ctacagccgc
     7741 gtggtcgata ttccgcaaat gggtactcat tccaccgccg ataccgtcgg tttaaccgcg
     7801 cttggcgtgg tggcagcagc tgttggtgtg cacgcagttg ccagcgccgt tgaccaacgc
     7861 agacgtcata accagcaacc tacagaaacc gaacatcagc caggcaatga ggataaacag
     7921 gcatgagcac tcagtacgaa actcagggat acaccatcaa taatgccgga cgccgcctgg
     7981 tggtcgaccc gattacgcgc atcgaaggcc acatgcgctg tgaagtgaat attaacgatc
     8041 agaatgtgat caccaatgcc gtctcctgcg gcaccatgtt tcgcgggctg gagatcatcc
     8101 tgcaagggcg cgacccgcgc gatgcgtggg cgttcgttga acgtatctgc ggcgtctgta
     8161 ctggcgtaca cgccctggct tcggtttacg ccatcgaaga tgccatcggt attaaagtgc
     8221 cggacaacgc caatatcatc cgcaacatta tgctggcaac gctctggtgc cacgatcatc
     8281 tggtgcactt ctatcagctt gccgggatgg actggatcga cgtgttagat gcgctgaaag
     8341 ccgacccgcg aaaaacctcc gaactggcgc aaagtctctc ctcttggccg aaatcatccc
     8401 ctggctattt cttcgacgta caaaatcgcc tgaagaaatt cgttgaaggc gggcagttgg
     8461 ggatcttccg caatggctac tgggggcacc cgcagtacaa actgccgcca gaagccaacc
     8521 tgatgggctt tgcccactat ctcgaagctc tcgatttcca acgtgaaatt gtcaaaattc
     8581 acgcggtctt tggcggtaaa aacccgcatc caaactggat tgtcggtggg atgccttgcg
     8641 ccattaacat tgacgaaagc ggcgcggtcg gggcggtcaa tatggaacgc ctgaacctgg
     8701 tgcagtcgat tatcacccgc acggcagact tcattaacaa cgtgatgatc cccgacgcct
     8761 tagccatcgg tcagttcaac aaaccgtgga gcgaaatcgg cacgggtctt tctgataaat
     8821 gtgtcctcag ctacggcgca ttcccggata ttgccaacga ctttggtgag aaaagtctgc
     8881 tgatgcctgg cggcgcggtg attaacggcg acttcaacaa tgtgctgcca gtggatttgg
     8941 ttgatccgca gcaggtgcag gagtttgtcg atcacgcctg gtatcgttat cccaacgatc
     9001 aggtcgggcg tcatccattc gatggcatta ccgacccgtg gtacaacccc ggcgatgtca
     9061 aaggcagcga taccaacatt cagcagctga atgaacagga acgctactcg tggatcaaag
     9121 cgccacgctg gcgcggtaac gcgatggaag tggggccgct ggcacgcacg ttaatcgctt
     9181 atcacaaagg cgatgctgcg accgttgagt cggtcgatcg catgatgtcg gcgttgaacc
     9241 tgccgctttc cggtatccag tcaacgttgg gccgcatttt gtgccgcgcg cacgaagcgc
     9301 agtgggccgc aggtaagttg cagtatttct tcgacaagct gatgaccaac ctgaaaaacg
     9361 gcaatctcgc cactgcttcc actgaaaaat gggaacctgc aacctggccg acagagtgcc
     9421 gtggcgtcgg ctttaccgaa gcgccgcgcg gagcgttagg ccactgggcc gccattcgcg
     9481 atggcaagat tgatctctac cagtgcgtgg tgccgaccac ctggaacgcc agtccgcgcg
     9541 atcccaaagg gcagattggc gcttatgaag cggcgctgat gaacaccaaa atggcgatcc
     9601 ccgagcaacc gctggagata ctgcgtactc tgcacagctt tgatccgtgc ctcgcctgtt
     9661 caactcatgt gctgggcgac gacggtagcg agctgatctc cgtgcaggtg cgttaacagc
     9721 gaaggagaat catcatgcaa cagaaaagcg acaacgttgt cagccactat gtctttgaag
     9781 caccagttcg tatctggcac tggctgacgg tgttatgcat ggcggtgttg atggtcaccg
     9841 gttactttat cggcaagccg ctaccttccg tcagtggcga ggcgacgtat ctgttctata
     9901 tgggttacat caggttaatt cacttcagcg ccgggatgat ttttaccgtg gtcttgctga
     9961 tgcggatcta ctgggctttt gttggcaatc gttactcccg cgagctgttt atcgtgccgg
    10021 tatggcgtaa aagctggtgg cagggcgtgt ggtatgaaat ccgctggtat ctgtttctgg
    10081 caaaacgtcc gagtgccgat ataggccata accccatcgc ccaggcggcg atgttcggct
    10141 atttcctgat gtcggtcttt atgatcatca ccggttttgc gctgtacagc gaacacagcc
    10201 agtacgctat ttttgcgccg ttccgttatg tggtggaatt tttctactgg acgggtggca
    10261 actcaatgga cattcacagc tggcatcggc tggggatgtg gctgattggg gcgtttgtga
    10321 tcggtcatgt ctacatggcg ctgcgtgaag acatcatgtc cgacgacacg gtgatctcca
    10381 ccatggtcaa cggctaccgt agccacaaat ttggcaaaat aagtaacaag gagcgttcat
    10441 gagcgagcaa cacgtggtgg tcatggggct gggcaacctg ctgtgggccg atgaaggctt
    10501 cggcgtgcgg gtggcggaac ggctgtatgc ccattaccac tggcccgagt atgtggagat
    10561 tgtcgatggc ggtactcagg gactgaactt gctggggtat gtcgaaagcg ccagccatct
    10621 gttgattctc gatgccattg actacgggct ggaacctgga acgctgcgaa cctatgccgg
    10681 agaacgcatt ccggcctatc tcagcgcgaa gaaaatgagc ctgcatcaga acagtttctc
    10741 cgaagtgctg gcgctggcag atatccgcgg acatctgcca gcacatattg ccctcgtcgg
    10801 gctgcaaccc gcgatgctcg acgactacgg cggcagcctg agcgaactgg cacgggagca
    10861 actgcccgct gcggaacaat cggcgctggc gcagttggct gcgtggggaa gtgtgccgca
    10921 accggctaat gaatcgcgtt gtctcaatta tgactgtctg tcgatggaaa attacgaagg
    10981 cgttcgcttg cgccagtacc ggatgacaca ggaggagcag ggatgagcaa cgacacgcca
    11041 tttgatgcgt tgtggcaacg aatgctggcg cgcggctgga cgccggtcag tgaatcccgt
    11101 cttgacgact ggcttacgca agcgccagac ggcgtggtgt tattaagcag tgacccgaaa
    11161 cgcacgccag aggtcagcga taatccggta atgattggcg aattactgca cgagtttccc
    11221 gactatacat ggcaggtggc gattgctgac cttgagcaga gcgaagccat cggcgatcgc
    11281 tttggcgcct ttcgctttcc tgccacttta gtgtttaccg gcggaaacta tcgcggcgtg
    11341 ctgaatggta ttcacccgtg ggcggaactg ataaacctga tgcgcgggct tgtcgaaccg
    11401 cagcaggagc gtgcctcatg agcgaaactt ttttccatct gctggggcca ggaacgcaac
    11461 cgaacgatga cagtttcagc atgaatccac tgccgatcac ctgtcaggtg aatgatgaac
    11521 cgagtatggc ggccttggag caatgtgctc acagcccgca ggtgattgcg ctgttaaacg
    11581 agttacaaca tcaactaagc gaacgccagc cgccgttggg cgaggtgctg gcagtcgatc
    11641 tgttaaatct caacgccgac gatcgtcact ttatcaatac gcttctcggg gaaggggaag
    11701 tgtcagtgcg cattcagcag gctgacgaca gtaaaagtga aatacaggag gcgatcttct
    11761 gcggattatg gcgggtgcgc agacgtcgcg gcgaaaagtt gctggaggac aaactggagg
    11821 ctggctgcgc gccgctggcg ttgtggcagg cggcaacgca aaatctcttg ccgacagatt
    11881 cgctgttacc gccgcccatt gatggcctga tgaatggcct accgttggcg catgagttac
    11941 tggcacatgt acgtaacccc gacgcgcagc cgcacagcat taatctgacg caattaccca
    12001 tcagcgaggc tgatcggctt tttctctcgc gtctctgtgg gccgggaaat attcagattc
    12061 gtaccattgg ctatggcgag agctatatca acgccacggg gttacgccat gtctggcatt
    12121 tacgctgtac ggacacctta aaaggcccgt tactggaaag ttatgaaatc tgcccaatac
    12181 cggaagtggt gctggcagcg ccagaagatt tggtcgactc tgcgcagcgg cttagcgagg
    12241 tatgtcagtg gctggcggaa gctgcaccga cgtaaaaaga cggtaagtat cgctttcagt
    12301 cttatgaata tcgcaatcgg cgaatacctc tggtcgtaga gtttcaggat aaagagggag
    12361 atctaacatt atcgggttat ttttctctct tcgcctacag gagtgcgcat gtgggatgtc
    12421 attgatttat cgcgctggca gtttgctctg accgcgctgt atcacttttt atttgtaccc
    12481 cttaccctgg ggctgatttt tttgctggct atcatggaaa ccatttacgt ggttaccggc
    12541 aaaacaatct accgcgatat gacgcgcttc tggggtaagc tcttcggtat caattttgct
    12601 cttggcgtgg ctaccggcct gaccatggag tttcagtttg gtactaactg gtcattctat
    12661 tccaactatg tgggcgatat ttttggcgca ccactggcga tggaagcatt aatggccttc
    12721 ttcctcgaat ccacctttgt cgggctgttc ttcttcggct ggcaacggct gaataaatac
    12781 cagcacctgc tggtgacgtg gctggtggcg ttcggttcaa atctctctgc attgtggata
    12841 ttgaatgcca acggttggat gcaatacccg accggtgcgc attttgatat cgacaccctg
    12901 cgtatggaga tgaccagctt cagcgagctg gtctttaacc cggtcagcca ggtgaaattt
    12961 gtgcacaccg tcatggcggg ctacgtgacc ggggccatgt ttattatggc gatcagcgcc
    13021 tggtatttac tgcgcggacg ggagcgcgat gtcgcattac gctcgtttgc catcggttcc
    13081 gtcttcggta ctctggcgat tatcggtacc ctgcaactcg gagacagttc tgcgtatgaa
    13141 gtcgcgcaag tacaaccggt aaaactggcg gcgatggaag gggagtggca aacggaacct
    13201 gctcctgcac cgttccatgt ggttgcctgg ccggaacagg atcaagagcg taacgccttt
    13261 gccatcaaaa ttcccgcgct gctagggatc ctcgccactc actcattaga taaacccgtg
    13321 ccgggtctga agaatttgat ggctgaaacc tacccacgct tgcaacgcgg acgtatggcc
    13381 tggctgttaa tgcaggaaat atctcaaggc aatcgtgagc cgcatgtgtt gcaggcattc
    13441 cgggagctgg aaggcgacct tggttacggc atgttgctct cccgctatgc gccggatatg
    13501 aatcatgtca cagccgcaca gtaccaggcg gcgatgcgtg gcgcgatacc tcaggttgcg
    13561 ccggtattct ggagtttccg catcatggtg ggctgtggtt ccctgctgct actggtgatg
    13621 ctgattgcgc ttgtccagac gctgcgtggc aaaatcgacc agcatcgctg ggtgctgaaa
    13681 atggcgctct ggagtttgcc gttgccgtgg attgcgattg aagccgggtg gtttatgacc
    13741 gagtttggtc gtcagccgtg ggcgatacag gacatcttac cgacatactc cgcgcactcc
    13801 gctttaacca caggacaact ggctttctca ctgatcatga tcgtagggct ttacaccctg
    13861 ttcttaatcg ccgaagtcta cctgatgcag aaatatgccc gtctggggcc gagcgcgatg
    13921 cagagtgaac aaccgacgca gcaacagggg taaaggagaa aatcatgttt gattatgaaa
    13981 cattgcgctt catctggtgg ctgctgattg gcgtgatcct ggtggtcttt atgatctccg
    14041 acggatttga catggggatc ggctgtttgc tgccgctggt ggcgcgtaat gatgatgaac
    14101 gccggatagt gattaatagc gttggtgcgc actgggaagg caatcaggtc tggttgatcc
    14161 tcgcaggtgg ggcattattt gcagcctggc ccagagtgta tgcagcggcg ttttccggct
    14221 tttatgtggc gatgatcctg gtgctgtgct cgctgttctt ccgtccgctg gcctttgatt
    14281 atcgcggaaa aattgccgat gcacgctggc gtaaaatgtg ggatgccggt ctggtcatcg
    14341 gcagtctggt gccgccggta gtcttcggta tcgccttcgg caacttgttg ctcggcgtgc
    14401 cgtttgcctt cacaccgcaa ttacgcgtgg agtatctcgg cagcttctgg caactgctga
    14461 cgccattccc tttattgtgt ggtttgctca gccttgggat ggtgattttg caaggtggcg
    14521 tctggttaca actgaaaact gttggtgtga ttcatctgcg ttcacagctg gcgactaaac
    14581 gcgctgcact gttggtgatg ctgtgctttt tgctggcagg ttactggctg tgggtcggta
    14641 ttgatggctt tgtactgctc gcccaggatg ctaacggtcc ttcccatccg ttaatgaaac
    14701 tggtggcagt gctacctggt gcctggatga ataatttcgt cgaatcgccc gttttgtgga
    14761 tcttcccgct gctggggttc ttctgcccat tgctgacggt gatggcgatt tatcgtggtc
    14821 gcccgggttg gggatttttg atggcatcat tgatgcaatt tggcgtgatt ttcacggcag
    14881 gcatcacgct gttccccttt gtcatgccgt caagtgtgag tccaatctcc agcctgacgt
    14941 tgtgggacag cacttccagt cagctgacgc tgagcattat gttggtaatc gtgctgatat
    15001 ttttgcccat tgtgttgctc tacactctct ggagctacta caaaatgtgg ggacgcatga
    15061 caacagaaac tctccgccgt aacaaaaacg agttgtacta aggagcagga acatgtggta
    15121 tttactttgg ttcgtcggca ttttgttgat gtgttcgctc tccacccttg tgttggtatg
    15181 gctggacccg cgtctgaaaa gttaacgaac gtaggcctga tgcggcgcat tagcatcgca
    15241 tcaggcaatc aataatgtta gatatgaaaa gcggaaacat atcgatgaaa gcgatcttaa
    15301 tcccattttt atctcttctg attccgttaa ccccgcaatc tgcattcgct cagagtgagc
    15361 cggagctgaa gctggaaagt atggtgattg tcagtcgtca tggtgtgcgt gctccaacca
    15421 aggccacgca actgatgcag gatgtcaccc cagacgcatg gccaacctgg ccggtaaaac
    15481 tgggttggct gacaccgcgc ggtggtgagc taatcgccta tctcggacat taccaacgcc
    15541 agcgtctggt agccgacgga ttgctggcga aaaagggctg cccgcagtct gctcaggtcg
    15601 cgattattgc tgatgtcgac gagcgtaccc gtaaaacagg cgaagccttc gccgccgggc
    15661 tggcacctga ctgtgcaata accgtacata cccaggcaga tacgtccagt cccgatccgt
    15721 tatttaatcc tctaaaaact ggtgtttgcc aactggataa cgcgaacgtg actgacgcga
    15781 tcctctgcag ggcaggaggg tcaattgctg actttaccgg gcatcggcaa acggtgtttc
    15841 gcgaactgga acgggtgctt aatttcccgc aatcaaactt gtgccttaac cgtgagaaac
    15901 aggacgaaag ctgttcatta acgcaggcat taccatcgga actcaaggtg agcgccgaca
    15961 atgtctcatt aaccggtgcg gtaagcctcg catcaatgct gacggagata tttctcctgc
    16021 aacaggcaca aggaatgccg gagccggggt ggggaaggat caccgattca caccagtgga
    16081 acaccttgct aagtttgcat aacgcgcaat tttatttact acaacgcacg ccggaggttg
    16141 cccgcagtcg cgccaccccg ttattggatt tgatcatggc agcgttgacg ccccatccac
    16201 cgcaaaaaca ggcgtatggt gtgacattac ccacttcagt gctgtttatt gccggacacg
    16261 atactaatct ggcaaatctc ggcggcgcac tggagctcaa ctggacgctt ccaggtcagc
    16321 cggataacac gccgccaggt ggtgaactgg tgtttgaacg ctggcgtcgg ctaagcgata
    16381 acagccagtg gattcaggtt tcgctggtct tccagacttt acagcagatg cgtgataaaa
    16441 cgccgctgtc attaaatacg ccgcccggag aggtgaaact gaccctggca ggatgtgaag
    16501 agcgaaatgc gcagggcatg tgttcgttgg caggttttac gcaaatcgtg aatgaagcac
    16561 gcataccggc gtgcagtttg taatgcataa aaaagagcat tcagttacct gaatgctctg
    16621 aggctgatga caaacgaaga actgtctaat gcgtagaccg gaaaaggcgt tcacgccgca
    16681 tccggtcact ttcagtttta ctctttctcg gagtaactat aaccgtaata gttatagccg
    16741 taactgtaag cggtgctggc gcgtttaatc acaccattga ggattgcgcc tttaatatta
    16801 acgccagcct gttccagacg ctgcattgac aagctcacct ctttggcggt gttcaagcca
    16861 aaacgcgcaa ccagcaggct ggtgccgaca gagcgtccca cgaccgcagc attgctcacc
    16921 gccagcatcg gcggcgtatc gacaatcacc agatcgtaat ggtcgttcgc ccattccagt
    16981 aattgacgca tccgatcgcg catcagcagt tcagacgggt taggtggcac ctgaccgcga
    17041 gtaatcacat caaagtctcc tttgccaaaa tgctggatca ctttgttgag ctcatcttta
    17101 cctgccagat attccgacaa gccatgttca ttactcacgg taaacaggtt atgcgaataa
    17161 ccacggcgta agtcggcatc aataaataac actttttgat cggactgggc gatcaccgct
    17221 gccagagttg aactgacaaa cgttttacca ctgtctggcg tcgcaccggt gatcatcaga
    17281 atgttattct cagtctccat catagcgaaa tgcagactgg ttcgtagcgc acgtacggct
    17341 tccacagcag aatccgccgg gttatccacc gccaggaagg ggatattttt agtacgatgg
    17401 cgctgctgat tagaaaataa atttttctta cgcagacggg tgcgtttatc cagccactcg
    17461 gacattggga tagtggaata aacgctgatg ccgtgctctt ccagttgttc cggggcttct
    17521 acaccacgac gcaacatcgc acgcgccagc acggcactca ccgaaataaa caggccaaga
    17581 ataaaaccaa gcaccacatt caacgctttt ttcggtttca ctggctgcgg ctgagtgact
    17641 gccgggtcga taatccgcac gttaccaatg gcactggatt tcgaaatact caactcctgc
    17701 tggcggttaa gtaattgcag atataccgca cggcccgctt ctacgtcacg acttaaacgc
    17761 aacacttcct gttgggtgga aggcattgcc gatacccgct tattcaggcg tttgcgttct
    17821 tgctccagcg tctggcgttt ttccagcagc gcacgataag ttgggtgatc tttcttatac
    17881 agctgggaga tctctgcctc gcggaaggtc agctcattga gttgattatc aacgttcaca
    17941 atctgctcaa gaacggcttt ggcttccagg ttaaggtcaa ccgaatcgcg ctgctggcga
    18001 taaacgttga gtttttcttc cgcctggtcc agctcgctgc gcacttcagg taactggcgc
    18061 tgtaagaatt caaggctttg tgaatcctgc gccgcctggc gagcgatatt ctgttgcaaa
    18121 tagttgttag cgatgctgtt cagaatacga gtaatcaact ggggatcatc accagtcatg
    18181 gtaagttcca gcatcccgct ttctttactg cgttcgctaa cggtaaaggt ttcctgcaat
    18241 gcgttaatcg cttccagttc ggtacgctgg ctcaggacaa actgtgttcc tggtttggcc
    18301 ttaatgtccg cgatagtcag cgcaacgcca tctttttcca gacgctggcc gaccatacca
    18361 ttgacggtga actcttcacc ttccagtgta tagtggccgt tttccccaac cgtgagtgtc
    18421 agttgctgat cctgaccatt cagttgtgga atatgcatcc agctgatcgc cagctcacct
    18481 agtttttctt tggttaatct cgcccagccg cgacccacaa tcggaaaata cttctgctca
    18541 actatgtcgc gcagattcag ttcagcaatg gttttaccga gaatcatgcg cgattgcagc
    18601 agttggatct ccggtgcaga ctcgggcgat gagttaggga tcatatcgct caggccgctg
    18661 agaatggcgt tgccctgttt ttgctcaacc tggaccagag tatctgcctg ataaattggt
    18721 gtgcttaaca gcgatcagcg tgaataacgc ggtcacgctg ataataaact tacggtgatc
    18781 ccataactcg ccgaccagac gaagcagatc gatctcattt tcctgagtgc tgcctggtgg
    18841 cgtattcata tttttagttg tcatcgtatt ttataaactc aaattaccgg ctgaggcgct
    18901 tcgcccattc ctgactggcg cgctccaaca tactgtagac atgttcaaat gcgtcctgac
    18961 ttttacgata gggatccggg atctctttct gttccagcca ttgcccaaac agcattgtct
    19021 tcccgcggac ctcgggagca attgcggtaa cctgagcaat atgttccgac tccattgcca
    19081 gaatcagatc gtaatttcgt gccatctcag cagtgagctt acgtccggca tgtccttcaa
    19141 gagatacgcc atgattagcg gcaacatctg ccgctgtcgc atctgcaggg tgttttacca
    19201 gaccatgaac acctgccgat ttgactttta cgcccggcag acgtttacgc agcaagcgct
    19261 cgccaatggg cgaacggcaa atgttgccgg tacaaaccac taggattgag ttaaatttta
    19321 gttgggccat gtttgaatcc accgtgaagt ctctgtcagg tcatgcaccc cggaaatcgt
    19381 cggtaccagc agagaaatca cacggttcca acgagccagc ggcgcggtag tgacataaac
    19441 aatgtcgtaa ggttcaagct ggaattccgt tccgagtatc attgccgatg cgtccttcgc
    19501 attcagctga taaatattgg cgattttgcc attctgcttg ttttgcgttg cacgaataac
    19561 aaaaatcccc gtcgcatcag cgacatcctg attcataccc tcagcgttac ccagcgcttc
    19621 tgccagggtc ataccgctgc ggtccatttt cagcgtgctc tgtttaccca cttcacccat
    19681 cacgaagact ttcagatcat cgttgcgcgg aataaataaa atatcgcccg ggtgcagcag
    19741 tttgttctgg cgcaagtcgc cacgctgcat cagcgcatag aggttcactt tggtctttac
    19801 gccattctgg gtgagcacca cgttgcgcca gtctgcatct gcggtcagac cgcccgcggc
    19861 gttaatcgca tccatgatgg tcagaggaat attggtaatc ggctgctgac ccgatttgct
    19921 gacttcgccg gtgacgtacg ctttttgcga tcggaacgcc gcgacgctga catcgacttg
    19981 cgggctttca atgactgaat cgagacgcgc tgtaatctcg ttgcgtacct gggtaagtgt
    20041 tttcccggcc actttcaggc gaccaatata ggggtagaaa atcgcaccat cggcgttaac
    20101 ccagttgccg gtatcactgg cgctacggta ttgccctgcg ggggtggtta gttccgggtg
    20161 atcccagacc gtaaccatca acacatcacc aatcccgata cgatattcat agtttgcgat
    20221 ttgctgctcc agctccgggt tagcctgcga cataatcggt ttggcgcgca gctgatcaat
    20281 caatcctggc gtgacgggat agatattgac cattttgtcc aggtcatact gattgtcagg
    20341 tagttcaatg acatccttgt tactggtcga taaattctga ccaggaacaa gggtacatgc
    20401 tgtcaaagac agaacagcca atgtcagtac cgaaaactta aaaatgttct ttttcattgg
    20461 tttatattta tcagtcaatg taatgttatg aattacgtca ggttattgtc caaccggtac
    20521 ttctcgctct gccgtcattg gatcgagctg gtatttgcgt gacagcattt gtccaccatc
    20581 acgcgtcaat ggtgtccacg aaaccaccgc gcggttgcgg ttaggtccaa tggtcatcaa
    20641 gtcgaacgga atcgagatat aaaaaccttt actaaagccg ccttcgccgt agtcatcttt
    20701 agatacgtta ctgattgccg cccatacccc taccgccacg ccgctgtcaa agagtttggc
    20761 gacgtcgatc gttgcgcctt tatcttttgc cagatattgc ccaacgctaa gtttcatcag
    20821 tacgccattg agcgtcggcg ggttccagta agccgtcacg aaacctgtgg gcgtggaata
    20881 atcggtgaag cgcatcatgt tgtcccagtc acgctgcttc acgtagttaa cgtccacacc
    20941 cagcgcccag ctggcatcta gcgggcgata aagaagctcg gaaccgacac cggcatacat
    21001 cgtttccaga taaccgccat acacctggcc atagaaacca ttgcctaagt cggcaaagta
    21061 attcgcttgc aagttgttga gataaacgtc attgcgaacg taatcacgga tatgcgtgcg
    21121 cacgcggggc agggtagagt ccgcgggtaa cagtgaagac ttaaacttgt cgtagttgtt
    21181 gtaaatattg gtgaaaatac cgtcatcaag cagcaggtgg tcggtaaacc agtagcgggc
    21241 gctggacatc agccccagct ggaacatata gaaatcttcc gggccgccca gcgactgtga
    21301 aagtgttggg ttgaaagagt agctaaagcg atcttcacga atacggtagc accgaccaaa
    21361 ggcagaaaga tcttctgctt caacacgttg ttgttgcagt tgctctgatt taccaggcgc
    21421 tgtgccggcc agctgtttgc gcaggctggc tacgtcggtt tcggtagtca ccatcgccat
    21481 atgctcgcgc ttttgcgtca cgctaatctt ctcaacgcct tgcggcaggt tattcaccag
    21541 aatccggttg gcacgatcga ccgcttcgcg agagtcacgg tatttgtatt gctgaccaga
    21601 catatacagc gtcttatcgc gtagctgaat ttctggcgcg tcaaagcccg cgttatactt
    21661 cagggcggta agttggtttg ctaccgtggt gtactgcaaa ccttcagact ctggcgcagg
    21721 ctgccaggcc ggttttggcg tatcgcgtag ggcagggcgc agatcgttga aattggtccg
    21781 taaagtgaaa ccaaacatca acgtgtttcc gcgttcataa ctgaggttga ggtctgccca
    21841 gctggcagcg cgataaactg caccgacgtt gaaatggctt gcctgaggca gtttgccagc
    21901 gaaatcattc tggtaattgt tgccgtcgta ttcgagtttc agacgcaggg gattccacgg
    21961 cgtttgatac tcaatgccgc caaagatgga agccgggcca cgaaagatat cgctaaagct
    22021 gatatcgccc gcatcgtgag actctgcgcg atgacaatat ttatcgctca cccggcaaaa
    22081 cgggttggta atattgcccg cattaccggc gtagccccat gccatcccga gggtgaaatc
    22141 aaatggcccc gcttgcttgc tggccaccag atattcaccg tcaaacaaac ccgtaccagc
    22201 aatatcacgt ttaccaaacg ccacttgcgg tagccagtaa ccttcttccc acaggcgaag
    22261 tttaaaatcg aatgatttgt ctttgtatga ctgatcgccg ctgaaatctt cccactggct
    22321 atatttgcgt gtgcgcacat ccgtataacg aatggtgcct tccagccatg ggaacagcgc
    22381 cacggaggtg gagtagaacc ggtattgatc gttatcccgg tagttgacgc tgaattcacc
    22441 ttccggtgcg atgcgcgcat ttggcatctg caacaatcct gtgccgccaa agtctgattg
    22501 cgacgaaccc agcggatccg ggtaggttaa tacttcagca tgacaggcac tggagacggc
    22561 aatggccagg cagcttaaaa gataagaatt cttcttcatc agtcaggaac acgttgcgtt
    22621 aaaacagaca caatctggtc atttaaatca gcatattttt cgggcagtac atgcgcagaa
    22681 aagcccaacc aaagctggct gccaggcggt ggttccacat gtcttttatt ccacagggca
    22741 acaggggcga cgacagtctc accctcaggc gtaatcacca taacgttatt cttgtctgcg
    22801 cctgcaaggc gggggtgatc ttgcagataa tcggtaacgc tacggcctgc ctgccagggc
    22861 aattgcccag ccccagaaac cgcacccagc agagtaatag tcaccggacg ctgtacggta
    22921 tagagcgtat agtccccgac caacggggga ttgctgttct catccactcg tacaaaatca
    22981 gggtccagtt tgaccggtaa gcgaccagtg atattgagat tgagtaactg ctggcgcacg
    23041 gatttaatcg ttgccgccac atcgtcgtca gcttctgctt cccaggaagc aagctgtgcc
    23101 atgacatgtt ggtagtcttt aagcgcttta gctttcgccg cagagtcggt cagcaaggca
    23161 ccaggccacc agaggcgatc gcgcagttgc ggttgtgtca ccaactggac aacattttcc
    23221 acggggccaa cggaaagtgt ctgttgttcg ctaggcagat aaatagtcac cgttccttgc
    23281 gcaaaggcat ggggtgtcat tacgtaaagt acgctggcaa tgaaatacga ctgtaattta
    23341 ttcatggtgc gggtttaaga aacgtcattt ctacgggaat aacccctgcg cctaacattt
    23401 gccgactttg acgaacctgg ccagtagccg aatcgaccca gaatgtgttt tgccactgtt
    23461 tttctggcag cgtggaagtc acctcttcct gccagacatt acacagcaca ttgtgacccg
    23521 caatattcag cgtctctaat ccagcaaagg aaaaagtaga acttagggtc gcagagcgaa
    23581 aatggttgtc ttcactccag agaatatcgc gcgtccagcg gctgccatct tttattgcca
    23641 gcgcgttacg cagaggggcc cgcccgggat ttgtcacttc cagcagatta ttattaagtt
    23701 tgacggtctt taaaagacgt ccattgtggg taaccagcat ggcgttatcc tgggataacc
    23761 atttgctttg ttcttgttct atatatccca gtaccacaaa gattcgttgc ccttcattaa
    23821 ggcgtaaata catcgtggaa taaggcaacg cctggatctg ctgagccgct acagtgatat
    23881 cctgattatc gaaaaggctg gcgcgaaatg tatcgaccat actttgctgg ctgtgcgtac
    23941 atcccgcaag aaatagtgcg aaaatcgata aaataagagg gcgcaccttt aatccttgaa
    24001 aggttaatta aaaaaatgca aatctctaaa gcccggtacg aatatcgcaa ccgggtatta
    24061 tttaacttga atttcgacgc gtttaagcag aattagcgcg tagtggacgt ggtggttgag
    24121 gtgttatgag aaccgccatc gccactgtca tttgctgcaa ctactgccaa cgtcgccaca
    24181 ccaacagccg ttgccacacc gacaactgtc gccgtgccga cagcgccagc agccacgcca
    24241 ctagtggtag ccgcagtcgt ggttgcacct gtagtggtcg ttgtggtgcc agtagtaccc
    24301 gtcgttgcct cggtcccgtt tgctgcttca ggggcagcaa acgcagctgg cgtcgtcaac
    24361 atgaaggcca taaggattgc agaaagcttg tgtttcataa cttttccttt attcatcgca
    24421 tggacaatac gtacgcttga gatcaggatt gtctactttg agtataacat ccttgtgaaa
    24481 tctccctttc ctcataccgc tagcactata tactgcgggt gggaaaggcg cgcagaggga
    24541 aatataagat tgttaacgat ttccctctgg aaatacgact tgtgagcata agataaatct
    24601 tagaaaaata cgaattaaag ataagaaaat tgttacgaaa gctattaatt attgttggta
    24661 atatagtttc aagtggtact ataactggct gttaaatggc atttataact attaggtgca
    24721 gagatattcg cttaaatgga ttagtttctt agtggaaaag taactgatgt tataatgtat
    24781 ggtgttgact attgaactat tccaataaaa ccagtcagct ttaaacaagc agcgtcatat
    24841 taagagagat aaacatttgc cgctgttggt cctcgcaggc catttacgcg gcaaaattcc
    24901 acgcgtaatc ctggtataag cacttctgcg tcgcggggag tgaatgcgga aatatggacc
    24961 tggacttctt tacgaccgtc ggaggggata atgaatcctt tgccgctttt gcgatcaaag
    25021 gttttgacaa ttcctgtcat tttacgggac aaacaaattc cttactgaaa atactgcgct
    25081 gcactatacg gggttaataa aataaagcca gcgatattta agaccgccgg acggctaaaa
    25141 taaaatttgc ttaatctcaa ttatcatgcc ttaatagctg cgtcggtttg aaagacagac
    25201 agcatacaaa gtagtttact aaagcagttc tcattatcag gcattatccc cttcttttga
    25261 gtctctctcc tgaacactaa gtggtttctg tattaaagcc ctgtttgccg aaaggcccaa
    25321 aatgaaggaa gtaaaatatg tctaataa
//
LOCUS       DAZSNM010000037        24905 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZSNM010000037 DAZSNM010000000
VERSION     DAZSNM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24905
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.037"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..79
                     /locus_tag="ACHW42_001296"
     CDS             <1..79
                     /locus_tag="ACHW42_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8943190.1"
                     /translation="RAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHW42_001297"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHW42_001297"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR8943191.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="ACHW42_001298"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="ACHW42_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR8943192.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHW42_001299"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHW42_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR8943193.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHW42_001300"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHW42_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR8943194.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHW42_001301"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHW42_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR8943195.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="ACHW42_001302"
     CDS             5377..5835
                     /locus_tag="ACHW42_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR8943196.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="ACHW42_001303"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="ACHW42_001303"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR8943197.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="ACHW42_001304"
     tRNA            6887..6971
                     /locus_tag="ACHW42_001304"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="ACHW42_001305"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="ACHW42_001305"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHW42_001306"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHW42_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR8943198.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHW42_001307"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHW42_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR8943199.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHW42_001308"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHW42_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR8943200.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="ACHW42_001309"
     CDS             complement(10252..13995)
                     /locus_tag="ACHW42_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR8943201.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="ACHW42_001310"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="ACHW42_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR8943202.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="ACHW42_001311"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="ACHW42_001311"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR8943203.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="ACHW42_001312"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="ACHW42_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR8943204.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="ACHW42_001313"
                     /gene_synonym="iatA"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="ACHW42_001313"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR8943205.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="ACHW42_001314"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="ACHW42_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR8943206.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="ACHW42_001315"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="ACHW42_001315"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR8943207.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="ACHW42_001316"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="ACHW42_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR8943208.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="ACHW42_001317"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="ACHW42_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR8943209.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="ACHW42_001318"
     CDS             23392..23499
                     /locus_tag="ACHW42_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR8943210.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="ACHW42_001319"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="ACHW42_001319"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR8943211.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..>24905)
                     /locus_tag="ACHW42_001320"
     CDS             complement(24538..>24905)
                     /locus_tag="ACHW42_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR8943212.1"
                     /translation="EHMLDGWLKGNISPSAELFYEDLDEADNIEVIRKLLDTLKASLM
                     EENQMELALRTSEALLQFNPEDPYEIRDRGLIYAQLDCEHVALNDLSYFVERCPEDPI
                     SEMIRAQINNIAHKHIVLH"
BASE COUNT         6249 a         6501 c         5983 g         6172 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgc
//
LOCUS       DAZSNM010000038        24652 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZSNM010000038 DAZSNM010000000
VERSION     DAZSNM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24652
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.038"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..386)
                     /locus_tag="ACHW42_001321"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="ACHW42_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="ACHW42_001322"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="ACHW42_001322"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR8943213.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="ACHW42_001323"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="ACHW42_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR8943214.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVNLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHW42_001324"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHW42_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR8943215.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="ACHW42_001325"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="ACHW42_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR8943216.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="ACHW42_001326"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="ACHW42_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR8943217.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="ACHW42_001327"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="ACHW42_001327"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR8943218.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="ACHW42_001328"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="ACHW42_001328"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR8943219.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHW42_001329"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHW42_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR8943220.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="ACHW42_001330"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="ACHW42_001330"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR8943221.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="ACHW42_001331"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="ACHW42_001331"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR8943222.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="ACHW42_001332"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="ACHW42_001332"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR8943223.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="ACHW42_001333"
     CDS             10748..11212
                     /locus_tag="ACHW42_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR8943224.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHW42_001334"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHW42_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR8943225.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /gene="selO"
                     /locus_tag="ACHW42_001335"
     CDS             12236..13672
                     /gene="selO"
                     /locus_tag="ACHW42_001335"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR8943226.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHW42_001336"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHW42_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR8943227.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACHW42_001337"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACHW42_001337"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR8943228.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACHW42_001338"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACHW42_001338"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR8943229.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="ACHW42_001339"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="ACHW42_001339"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR8943230.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACHW42_001340"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACHW42_001340"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFR8943231.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="ACHW42_001341"
     CDS             complement(20505..20798)
                     /locus_tag="ACHW42_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR8943232.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="ACHW42_001342"
     CDS             complement(20795..22084)
                     /locus_tag="ACHW42_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR8943233.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="ACHW42_001343"
     CDS             complement(22140..23078)
                     /locus_tag="ACHW42_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR8943234.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="ACHW42_001344"
     CDS             complement(23098..23862)
                     /locus_tag="ACHW42_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR8943235.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24180..>24652
                     /locus_tag="ACHW42_001345"
     CDS             24180..>24652
                     /locus_tag="ACHW42_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFR8943236.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT         6104 a         5950 c         6482 g         6116 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgaatctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    23941 gcgggggggg ggcgacggag caagctgttt tccttgccaa caatcttgca aaaaaaatgc
    24001 gattgctaaa acaaaagtgc aatttgcggc aatatagtta ttccatgacc gtacaatctt
    24061 gcattcagtg taaataatct tgtttagctc tcgctaaacg cggttgtata accgcaatac
    24121 acgccgtatt ttatccggca tatattgttt ttgcttgctc atggaatagg gaggttgtca
    24181 tgtatcaacg ctgttttgat aatgccagtg aaacgctgtt tgtcgccggt aaaacgccac
    24241 ggctttcacg ttttgcattt agcgatgatc caaaatggga gtctggacat cacgttcatg
    24301 acaatgaaac cgagctgatt tacgtcaaga aaggggttgc aaggtttacc atcgattctt
    24361 cgttatatgt cgcacatgcg gatgacattg tggtgataga acgcggcagg ctgcatgcgg
    24421 tggcctctga cgttaacgat ccggcaacga cgtgtacctg tgcgctgtac ggctttcagt
    24481 ttcagggggc tgaggaaaat cagttactgc aaccgcattc ttgtccggta attgccgcgg
    24541 ggcagggaaa agaagtcata aaaaccttat ttaatgagct aagtgtgatt ttgccgcaaa
    24601 gtaaaaatag ccaaacatct tcgttatggg atgcattagc ctatacatta gc
//
LOCUS       DAZSNM010000039        24562 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZSNM010000039 DAZSNM010000000
VERSION     DAZSNM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24562
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.039"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(2..94)
                     /locus_tag="ACHW42_001346"
     tRNA            complement(2..94)
                     /locus_tag="ACHW42_001346"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACHW42_001347"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACHW42_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR8943237.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACHW42_001348"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACHW42_001348"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR8943238.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACHW42_001349"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACHW42_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR8943239.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="ACHW42_001350"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="ACHW42_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR8943240.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="ACHW42_001351"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="ACHW42_001351"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR8943241.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="ACHW42_001352"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="ACHW42_001352"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR8943242.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="ACHW42_001353"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="ACHW42_001353"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="ACHW42_001354"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="ACHW42_001354"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR8943243.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="ACHW42_001355"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="ACHW42_001355"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR8943244.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="ACHW42_001356"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="ACHW42_001356"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFR8943245.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="ACHW42_001357"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="ACHW42_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR8943246.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="ACHW42_001358"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="ACHW42_001358"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR8943247.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="ACHW42_001359"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="ACHW42_001359"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR8943248.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="ACHW42_001360"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="ACHW42_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR8943249.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="ACHW42_001361"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="ACHW42_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR8943250.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="ACHW42_001362"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="ACHW42_001362"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR8943251.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="ACHW42_001363"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="ACHW42_001363"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR8943252.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="ACHW42_001364"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="ACHW42_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR8943253.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="ACHW42_001365"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="ACHW42_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR8943254.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20948..21019)
                     /locus_tag="ACHW42_001366"
                     /pseudo
     CDS             complement(20948..21019)
                     /locus_tag="ACHW42_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="ACHW42_001367"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="ACHW42_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR8943255.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="ACHW42_001368"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="ACHW42_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR8943256.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..>24562)
                     /locus_tag="ACHW42_001369"
     CDS             complement(23682..>24562)
                     /locus_tag="ACHW42_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HFR8943257.1"
                     /translation="LIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGHRDIEKLGG
                     IGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAV
                     GLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAICCVIGGVAA
                     PWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRLPSRSRGAA
                     WVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMA
                     LVELAVLVVIIVSRGA"
BASE COUNT         5795 a         6910 c         6299 g         5558 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cg
//
LOCUS       DAZSNM010000040        24456 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZSNM010000040 DAZSNM010000000
VERSION     DAZSNM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24456
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.040"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..136
                     /locus_tag="ACHW42_001370"
     CDS             <1..136
                     /locus_tag="ACHW42_001370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462771.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I associates with
                     the membrane and may be involved with cation
                     translocation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit I"
                     /protein_id="HFR8943258.1"
                     /translation="VLAMLVLLVVALAVLKAVFLPLIVTWVLVLVVQILAPAVINNKG
                     "
     gene            145..960
                     /gene="atpB"
                     /locus_tag="ACHW42_001371"
     CDS             145..960
                     /gene="atpB"
                     /locus_tag="ACHW42_001371"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR8943259.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            1007..1246
                     /gene="atpE"
                     /locus_tag="ACHW42_001372"
     CDS             1007..1246
                     /gene="atpE"
                     /locus_tag="ACHW42_001372"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR8943260.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            1308..1778
                     /gene="atpF"
                     /locus_tag="ACHW42_001373"
     CDS             1308..1778
                     /gene="atpF"
                     /locus_tag="ACHW42_001373"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR8943261.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            1793..2326
                     /gene="atpH"
                     /locus_tag="ACHW42_001374"
     CDS             1793..2326
                     /gene="atpH"
                     /locus_tag="ACHW42_001374"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR8943262.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            2339..3880
                     /gene="atpA"
                     /locus_tag="ACHW42_001375"
     CDS             2339..3880
                     /gene="atpA"
                     /locus_tag="ACHW42_001375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR8943263.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            3931..4794
                     /gene="atpG"
                     /locus_tag="ACHW42_001376"
     CDS             3931..4794
                     /gene="atpG"
                     /locus_tag="ACHW42_001376"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR8943264.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            4821..6203
                     /gene="atpD"
                     /locus_tag="ACHW42_001377"
     CDS             4821..6203
                     /gene="atpD"
                     /locus_tag="ACHW42_001377"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR8943265.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            6224..6643
                     /locus_tag="ACHW42_001378"
     CDS             6224..6643
                     /locus_tag="ACHW42_001378"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR8943266.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            6994..8364
                     /gene="glmU"
                     /locus_tag="ACHW42_001379"
     CDS             6994..8364
                     /gene="glmU"
                     /locus_tag="ACHW42_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR8943267.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            8526..10355
                     /gene="glmS"
                     /locus_tag="ACHW42_001380"
     CDS             8526..10355
                     /gene="glmS"
                     /locus_tag="ACHW42_001380"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR8943268.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            10517..11338
                     /locus_tag="ACHW42_001381"
     CDS             10517..11338
                     /locus_tag="ACHW42_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR8943269.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            11325..13433
                     /locus_tag="ACHW42_001382"
     CDS             11325..13433
                     /locus_tag="ACHW42_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR8943270.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            13430..15097
                     /locus_tag="ACHW42_001383"
     CDS             13430..15097
                     /locus_tag="ACHW42_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR8943271.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            15100..16626
                     /locus_tag="ACHW42_001384"
     CDS             15100..16626
                     /locus_tag="ACHW42_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR8943272.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            16627..18243
                     /locus_tag="ACHW42_001385"
     CDS             16627..18243
                     /locus_tag="ACHW42_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR8943273.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(18299..18451)
                     /locus_tag="ACHW42_001386"
                     /pseudo
     CDS             complement(18299..18451)
                     /locus_tag="ACHW42_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(18474..18851)
                     /locus_tag="ACHW42_001387"
     CDS             complement(18474..18851)
                     /locus_tag="ACHW42_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8943274.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(19261..19632)
                     /locus_tag="ACHW42_001388"
     CDS             complement(19261..19632)
                     /locus_tag="ACHW42_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8943275.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(19693..20190)
                     /locus_tag="ACHW42_001389"
     CDS             complement(19693..20190)
                     /locus_tag="ACHW42_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8943276.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(20219..21054)
                     /gene="aadA1"
                     /locus_tag="ACHW42_001390"
                     /pseudo
     CDS             complement(20219..21054)
                     /gene="aadA1"
                     /locus_tag="ACHW42_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            complement(21112..21636)
                     /gene="sat2"
                     /locus_tag="ACHW42_001391"
     CDS             complement(21112..21636)
                     /gene="sat2"
                     /locus_tag="ACHW42_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR8943277.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(21731..22204)
                     /gene="dfrA1"
                     /locus_tag="ACHW42_001392"
     CDS             complement(21731..22204)
                     /gene="dfrA1"
                     /locus_tag="ACHW42_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR8943278.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            22536..23513
                     /gene="intI2"
                     /locus_tag="ACHW42_001393"
                     /pseudo
     CDS             22536..23513
                     /gene="intI2"
                     /locus_tag="ACHW42_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            complement(23701..23940)
                     /locus_tag="ACHW42_001394"
     CDS             complement(23701..23940)
                     /locus_tag="ACHW42_001394"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR8943279.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         6392 a         5557 c         6184 g         6323 t
ORIGIN      
        1 agttctggcg atgttggtgt tactggtggt ggcgttggcg gttttaaagg cggtattctt
       61 gccgctgatc gttacgtggg ttttggtgct ggtggttcag atactggcac cggctgtaat
      121 taacaacaaa gggtaaaagg catcatggct tcagaaaata tgacgccgca ggattacata
      181 ggacaccacc tgaataacct tcagctggac ctgcgtacat tctcgctggt ggatccacaa
      241 aaccccccag ccaccttctg gacaatcaat attgactcca tgttcttctc ggtggtgctg
      301 ggtctgttgt tcctggtttt attccgtagc gtagccaaaa aggcgaccag cggtgtgcca
      361 ggtaagtttc agaccgcgat tgagctggtg atcggctttg ttaatggtag cgtgaaagac
      421 atgtaccatg gcaaaagcaa gctgattgct ccgctggccc tgacgatctt cgtctgggta
      481 ttcctgatga atctgatgga tttactgcct atcgacttgc tgccgtacat tgctgaacat
      541 gtactgggtc tgcctgcact gcgtgtggtt ccgtctgcgg acgtgaacgt aacgctgtct
      601 atggcactgg gcgtatttat cctgattctg ttctacaaca tcaaaatgaa aggcatcggc
      661 ggcttcacga aagagttgac gctgcagccg ttcaatcact gggcgttcat tcctgtcaac
      721 ttaatccttg aaggggtaag cctgctgtcc aaaccagttt cactcggttt gcgactgttc
      781 ggtaacatgt atgccggtga gctgattttc attctgattg ctggtctgtt gccgtggtgg
      841 tcacagtgga tcctgaatgt gccgtgggcc attttccaca tcctgatcat tacgctgcaa
      901 gccttcatct tcatggttct gacgatcgtc tatctgtcga tggcgtctga agaacattaa
      961 tttaccaaca ctactacgtt ttaactgaaa caaactggag actgtcatgg aaaacctgaa
     1021 tatggatctg ctgtacatgg ctgccgctgt gatgatgggt ctggcggcaa tcggtgctgc
     1081 gatcggtatc ggcatcctcg ggggtaaatt cctggaaggc gcagcgcgtc aacctgatct
     1141 gattcctctg ctgcgtactc agttctttat cgttatgggt ctggtggatg ctatcccgat
     1201 gatcgctgta ggtctgggtc tgtacgtgat gttcgctgtc gcgtagtaag cgttgctttt
     1261 atttaaagag caatatcaga acgttaacta aatagaggca ttgtgctgtg aatcttaacg
     1321 caacaatcct cggccaggcc atcgcgtttg tcctgttcgt tctgttctgc atgaagtacg
     1381 tatggccgcc attaatggca gccatcgaaa aacgtcaaaa agaaattgct gacggccttg
     1441 cttccgcaga acgagcacat aaggaccttg accttgcaaa ggccagcgcg accgaccagc
     1501 tgaaaaaagc gaaagcggaa gcccaggtaa tcatcgagca ggcgaacaaa cgccgctcgc
     1561 agattctgga cgaagcgaaa gctgaggcag aacaggaacg tactaaaatc gtggcccagg
     1621 cgcaggcaga aattgaagcc gagcgtaaac gtgcccgtga agagctgcgt aagcaagttg
     1681 ctatcctggc tgttgctggc gccgagaaga tcatcgaacg ttccgtggat gaagctgcta
     1741 acagcgacat cgtggataaa cttgtcgctg aactgtaagg agggaggggc tgatgtctga
     1801 atttattacg gtagctcgcc cctacgccaa agcagctttt gactttgccg tcgaacacca
     1861 aagtgtagaa cgctggcagg acatgctgac gtttgccgcc gaggtaacca aaaacgaaca
     1921 aatggcagag cttctctctg gcgcgcttgc gccagaaacg ctcgccgagt cgtttatcgc
     1981 agtttgtggt gagcaactgg acgaaaacgg tcagaacctg attcgggtaa tggctgaaaa
     2041 tggtcgtctt aacgcgctcc cggatgttct ggagcagttt attcaccttc gtgccgtgag
     2101 tgaggctacc gctgaggtag acgtcatttc cgctgccgca ctgagtgaac aacagctcgc
     2161 gaaaatttct gctgcgatgg aaaaacgtct gtcacgcaaa gttaagctga attgcaaaat
     2221 cgataagtct gtaatggctg gcgttatcat ccgatcgggt gatatggtca ttgatggcag
     2281 cgtacgcggt cgtcttgagc gccttgcaga cgtcttgcag tcttaagggg actggagcat
     2341 gcaactgaat tccaccgaaa tcagcgaact gatcaagcag cgcattgctc agttcaatgt
     2401 tgtgagtgaa gctcacaacg aaggtactat tgtttctgta agtgacggtg ttatccgcat
     2461 tcacggcctg gccgattgta tgcagggtga aatgatctcc ctgccgggta accgttacgc
     2521 tatcgcactg aacctcgagc gcgactctgt aggtgcggtt gttatgggtc cgtacgctga
     2581 ccttgccgaa ggcatgaaag ttaagtgtac tggccgtatc ctggaagttc cggttggccg
     2641 tggcctgctg ggccgtgtgg ttaacactct gggtgcacca atcgacggta aaggtccgct
     2701 ggatcacgac ggcttctctg ctgtagaagc aatcgctccg ggcgttatcg aacgtcagtc
     2761 cgtagatcag ccggtacaga ccggttataa agccgttgac tccatgatcc caatcggtcg
     2821 tggtcagcgt gaattgatca tcggtgaccg tcagacaggt aaaaccgcac tggctatcga
     2881 tgccatcatc aaccagcgcg attccggtat caaatgtatc tatgtcgcta tcggccagaa
     2941 agcgtccacc atttctaacg tggtacgtaa actggaagag cacggcgcac tggctaacac
     3001 catcgttgtg gtagcaaccg cgtctgaatc cgctgcactg caatacctgg caccgtatgc
     3061 cggttgcgca atgggcgaat acttccgtga ccgcggtgaa gatgcgctga tcatttacga
     3121 tgacctgtct aaacaggctg ttgcttaccg tcagatctcc ctgctgctcc gtcgtccgcc
     3181 aggacgtgaa gcattcccgg gcgacgtttt ctacctccac tctcgtctgc tggagcgtgc
     3241 tgcacgtgtt aacgccgaat acgttgaagc cttcaccaaa ggtgaagtga aagggaaaac
     3301 cggttctctg accgcactgc cgattatcga aactcaggcg ggtgacgttt ctgcgttcgt
     3361 tccgaccaac gtaatctcca ttaccgatgg tcagatcttc ctggaaacca acctgttcaa
     3421 cgccggtatt cgtcctgcgg ttaacccggg tatttccgta tcccgtgttg gtggtgcagc
     3481 acagaccaag atcatgaaaa aactgtccgg tggtatccgt accgctctgg cacagtatcg
     3541 tgaactggca gcgttctctc agtttgcatc cgaccttgac gatgcaacac gtaagcagct
     3601 tgaccacggt cagaaagtga ccgaactgct gaaacagaaa cagtatgcgc cgatgtccgt
     3661 tgcgcagcag tctctggttc tgttcgcagc agaacgtggt tacctggcgg atgttgaact
     3721 gtcgaaaatt ggcagcttcg aagccgctct gctggcttac gtcgaccgtg atcacgctcc
     3781 gttgatgcaa gagatcaacc agaccggtgg ctacaacgac gaaatcgaag gcaagctgaa
     3841 aggcatcctc gattccttca aagcaaccca atcctggtaa cgtctggagg cttgccttag
     3901 ggcaggccgc aaggcattga ggagaagctc atggccggcg caaaagagat acgtagtaag
     3961 atcgcaagcg tccagaacac gcaaaagatc actaaagcga tggagatggt cgccgcttcc
     4021 aaaatgcgta aatcgcagga tcgcatggcg gccagccgtc cttatgcaga aaccatgcgc
     4081 aaagtgattg gtcaccttgc acacggtaat ctggaatata agcaccctta cctggaagac
     4141 cgcgacgtta aacgcgtggg ctacctggtg gtgtcgaccg accgtggttt gtgcggtggt
     4201 ttgaacatta acctgttcaa aaaactgctg gcggaaatga agacctggac cgacaaaggc
     4261 gttcaatgcg acctcgcaat gatcggctcg aaaggcgtgt cgttcttcaa ctccgtgggc
     4321 ggcaatgttg ttgcccaggt caccggcatg ggggataacc cttccctgtc cgaactgatc
     4381 ggtccggtaa aagtgatgtt gcaggcctac gacgaaggcc gtctggacaa gctttacatt
     4441 gtcagcaaca aatttattaa caccatgtct caggttccga ccatcagcca gctgctgccg
     4501 ttaccggcat cagatgatga tgatctgaaa cataaatcct gggattacct gtacgaaccc
     4561 gatccgaagg cgttgctgga taccctgctg cgtcgttatg tcgaatctca ggtttatcag
     4621 ggcgtggttg aaaacctggc cagcgagcag gccgcccgta tggtggcgat gaaagccgcg
     4681 accgacaatg gcggcagcct gattaaagag ctgcagttgg tatacaacaa agctcgtcag
     4741 gccagcatta ctcaggaact caccgagatc gtctcggggg ccgccgcggt ttaaacaggt
     4801 tatttcgtag aggatttaag atggctactg gaaagattgt ccaggtaatc ggcgccgtag
     4861 ttgacgtcga attccctcag gatgccgtac cgcgcgtgta cgatgctctt gaggtgcaaa
     4921 atggtaatga gcgtctggtg ctggaagttc agcagcagct cggcggcggt atcgtgcgta
     4981 ccatcgcaat gggttcctcc gacggtctgc gtcgcggtct ggatgtaaaa gacctcgaac
     5041 acccgatcga agtcccggta ggtaaagcga ctctgggccg tatcatgaac gtactgggtg
     5101 aaccggtcga catgaaaggc gagatcggtg aagaagagcg ttgggcgatt caccgcgcag
     5161 caccttccta cgaagagctg tcaaactctc aggaactgct ggaaaccggt atcaaagtta
     5221 tcgacctgat gtgtccgttc gctaagggcg gtaaagttgg tctgttcggt ggtgcgggtg
     5281 taggtaaaac cgtaaacatg atggagctca ttcgtaacat cgcgatcgag cactccggtt
     5341 actctgtgtt tgcgggcgta ggtgaacgta ctcgtgaggg taacgacttc taccacgaaa
     5401 tgaccgactc caacgttatc gacaaagtat ccctggtgta tggccagatg aacgagccgc
     5461 cgggaaaccg tctgcgcgtt gctctgaccg gtctgaccat ggctgagaaa ttccgtgacg
     5521 aaggtcgtga cgttctgctg ttcgttgaca acatctatcg ttacaccctg gccggtacgg
     5581 aagtatccgc actgctgggc cgtatgcctt cagcggtagg ttatcagccg accctggcgg
     5641 aagagatggg cgttctgcag gaacgtatca cctccaccaa aactggttct atcacctccg
     5701 tacaggcggt atacgtacct gcggatgact tgactgaccc gtctccggca accacctttg
     5761 cgcaccttga cgcaaccgtg gtactgagcc gtcagatcgc gtctctgggt atctacccgg
     5821 ccgttgaccc gctggactcc accagccgtc agctggaccc gctggtggtt ggtcaggaac
     5881 actacgacac cgcgcgtggc gttcagtcca tcctgcaacg ttatcaggaa ctgaaagaca
     5941 tcatcgccat cctgggtatg gatgaactgt ctgaagaaga caaactggtg gtagcgcgtg
     6001 ctcgtaagat ccagcgcttc ctgtcccagc cgttcttcgt ggcagaagta ttcaccggtt
     6061 ctccgggtaa atacgtctcc ctgaaagaca ccatccgtgg ctttaaaggc atcatggaag
     6121 gcgaatacga tcacctgccg gagcaggcgt tctacatggt cggttccatc gaagaagctg
     6181 tggaaaaagc caaaaaactt taacgcctta atcggagggt gatatggcaa tgacttacca
     6241 cctggacgtc gtcagcgcag agcaacaaat gttctctggt ctggtcgaga aaatccaggt
     6301 aacgggtagc gaaggtgaac tggggatcta ccctggccac gcaccgctgc tcaccgccat
     6361 taagcctggt atgattcgca tcgtgaaaca gcacggtcac gaagagttta tctatctgtc
     6421 tggcggcatt cttgaagtgc agcctggcaa cgtgaccgtt ctggccgaca ccgcaattcg
     6481 cggccaggat ctcgacgaag cgcgagccat ggaagcgaaa cgtaaggctg aagagcacat
     6541 tagcagctct cacggcgacg tagattacgc tcaggcgtct gcggaactgg ccaaagcgat
     6601 cgcgcagctg cgcgttatcg agttgaccaa aaaagcgatg taacaccggc ttgaaaagca
     6661 caaaagccag tctggaaaca ggctggcttt ttttacgcgt tgacccgtcc tgaagagcgt
     6721 tcacatagat cctgctgata taaaacaccc ctgttttccc gtttattcat tgatcgaaat
     6781 aagagcaaaa acatccacct gacgcttaaa tgaaggtact gccttaattt tctgcagata
     6841 aaaggcgtga cgatggtcga aaatggcact ttcgtcagcg gggataatcc gttattgaac
     6901 aatttatcct ctgtccattt cacgatgaaa aaaatgtagt tttttcaagg tgaagcggtt
     6961 taaattcgtt ctcaaattac agtcaggacg cgtatgttga ataatgctat gagcgtagtg
     7021 atccttgccg caggcaaagg cacgcgcatg tattccgatc ttccgaaagt gctgcatacc
     7081 cttgccggga aagcgatggt tcagcatgtc attgatgctg cgaatgaatt aggcgcagcg
     7141 cacgttcacc tggtgtacgg tcacggcggc gatctgctta aacaggcgct gaaagacgac
     7201 aaccttaact gggtgcttca ggcagagcag ctgggtacgg gtcatgcaat gcagcaggcc
     7261 gcacctttct ttgccgatga tgaagacatt ttaatgctct acggcgacgt gccgctgatc
     7321 tctgtcgaaa cactccagcg cctgcgtgat gctaaacccc agggtggcat tggtctgctg
     7381 acggtgaaac tggatgatcc gaccggttat ggacgtatca cccgtgaaaa tggcaaagtt
     7441 accggcattg ttgagcacaa agatgccacc gacgagcagc gtcagattca ggagatcaac
     7501 accggcattc tgattgccaa cggcgcagat atgaaacgct ggctggcgaa gctgaccaac
     7561 aataatgctc agggcgaata ctacatcacc gacattattg cgctggcgta tcaggaaggg
     7621 cgtgaaatcg tcgccgttca tccgcaacgt ttaagcgaag tagaaggtgt gaataaccgc
     7681 ctgcaactct cccgtctgga gcgcgtttac cagtccgaac aggctgaaaa actgctgtta
     7741 gcaggcgtta tgctgcgcga tccggcgcgt tttgatctgc gcggtacgct tactcacggg
     7801 cgcgatgttg aaattgatac taacgttatc atcgagggca acgtgactct cggtcatcgc
     7861 gtgaaaatcg gcaccggttg cgtgattaaa aacagcgtga ttggcgatga ttgcgaaatc
     7921 agtccgtata ccgttgtgga agacgcgaat ctggcagcgg cctgtactat tggcccgttt
     7981 gcccgtctgc gtcctggtgc tgagttgctg gaaggtgcac acgttggtaa cttcgttgag
     8041 atgaaaaaag cgcgtctggg taaaggcacg aaagccgggc atctgactta cctgggcgat
     8101 gcggaaattg gcgataacgt taacatcggc gcgggaacca ttacctgcaa ctacgatggt
     8161 gcgaataaat ttaagaccat tatcggcgac gatgtgtttg tcggttccga cactcagctg
     8221 gtggccccgg taacagtagg caaaggcgcg accattgctg cgggtacaac tgtgacgcgt
     8281 aatgtcggcg aaaatgcatt agctatcagc cgtgtgccgc agactcagaa agaaggctgg
     8341 cgtcgtccgg taaagaaaaa gtaattctgg ccgggtaatc cggtcatctg ggatgaggag
     8401 ataacataat ctccctccca caagcagtaa ctataaaaat aaccccactc tctacaaggc
     8461 tcggggcgcc cgaaaaaacg ggcatacagg ttgaccgaca acgatataaa tcggaatcaa
     8521 aaactatgtg tggaattgtt ggcgcgatcg cgcaacgtga tgtagcagaa atccttcttg
     8581 aaggtttacg tcgtctggaa taccgcggat atgactctgc cggtctggcc gttgttgatg
     8641 cggaaggtca tatgactcgc ctgcgtcgcc tcggtaaagt ccagatgctg gctcaggcag
     8701 cggaagaaca tcctctgcat ggcggcaccg gtattgctca cactcgctgg gcgacccacg
     8761 gtgaaccttc agaagctaac gcgcatccgc atgtttctga acacattgtg gtggtgcata
     8821 atggcatcat cgaaaaccat gaaccgctgc gtgaagagtt aaaagcgcgt ggctatacct
     8881 tcgtttctga aaccgacacc gaagtgattg cccatctggt gaactgggag ctgaaacaag
     8941 gcgggactct gcgtgaggct gttctgcgtg ctatcccgca gctgcgcggt gcgtacggta
     9001 cagtgatcat ggactcccgt cacccggata ccctgctagc ggcacgttct ggtagtccgc
     9061 tggtgattgg cctgggtatg ggcgaaaact ttatcgcttc tgaccagctg gcgctgttgc
     9121 cggtgacccg tcgctttatc ttccttgaag agggcgatat tgcggaaatc actcgccgtt
     9181 cggtaaacat cttcgataaa actggcgcgg aagtaaaacg tcaggatatc gaatccaatc
     9241 tgcaatatga cgcgggcgat aaaggtattt accgtcacta catgcagaaa gagatctacg
     9301 aacagccgaa cgcgataaaa aacaccctta ccgggcgcat cagccacggt caggttgatt
     9361 taagcgagct gggaccgaac gccgacgaac tgctgtcgaa ggttgagcat attcagatcc
     9421 tcgcctgtgg tacttcttat aactccggta tggtttcccg ctactggttt gaatcgctgg
     9481 caggtattcc gtgcgacgtc gaaatcgcct ctgaattccg ctatcgtaaa tctgccgtgc
     9541 gtcgtaacag cctgatgatc accttgtcac agtctggcga aaccgcggat accctggcgg
     9601 gcctgcgtct gtcgaaagag ctgggttacc ttggttcgct ggcaatctgt aacgttccgg
     9661 gttcttctct ggtgcgcgaa tccgatctgg cgctgatgac caacgcgggt acggaaatcg
     9721 gcgtggcgtc cactaaagca ttcaccactc agttaactgt gctgttgatg ctggtggcga
     9781 agctgtctcg cctgaaaggt ctggatgcct ccattgaaca tgacattgtg catggtctgc
     9841 aggcgctgcc gagccgtatt gagcagatgc tgtctcagga taaacgcatt gaagctctgg
     9901 cagaagattt ctctgacaaa catcacgcgc tgttcctggg ccgtggcgat cagtacccaa
     9961 tcgcgctgga aggcgcattg aagctgaaag agatctctta cattcacgct gaagcctacg
    10021 ctgcaggcga gctgaaacac ggcccgctgg cgctgattga cgcggatatg ccggttatcg
    10081 tcgttgcacc gaacaacgaa ctgttggaaa aactgaaatc caacattgaa gaagttcgcg
    10141 cgcgtggcgg tcaattgtat gtcttcgccg atcaggatgc gggttttgta agtagcgata
    10201 acatgcacat catcgagatg ccgcatgtgg aagaggtgat tgcaccgatc ttctacaccg
    10261 ttccgctgca gctgctggct taccatgtcg cgctgatcaa aggcaccgac gttgaccagc
    10321 cgcgtaacct ggcaaaatcg gttacggttg agtaatcact atcatagata aaaaagggct
    10381 gctgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
    10441 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
    10501 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
    10561 tcgtattaaa gaggggcgtg gccaagggca tggtaaagac tatattccat ggctaacagt
    10621 acaagaagtt ccttcttcag gtcgttccca ccgtatttat tctcataaga cgggacgagt
    10681 ccatcatttg ctatctgact tagagcttgc tgtttttctc agtcttgagt gggagagcag
    10741 cgtgctagat atacgcgagc agttcccctt attacctagt gataccaggc agattgcaat
    10801 agatagtggt attaagcatc ctgttattcg tggtgtagat caggttatgt ctactgattt
    10861 tttagtggac tgcaaagatg gtccttttga gcagtttgct attcaagtca aacctgcagc
    10921 agccttacaa gacgagcgta ccttagaaaa actagaacta gagcgtcgct attggcagca
    10981 aaagcaaatt ccttggttca tttttactga taaagaaata aatcccgtag taaaagaaaa
    11041 tattgaatgg ctttattcag tgaaaacaga agaagtttct gcggagcttt tagcacaact
    11101 atccccattg gcccatatcc tgcaagaaaa aggagatgaa aacattatca atgtctgtaa
    11161 gcaggttgat attgcttatg atttggagtt aggcaaaaca ttgagtgaga tacgagcctt
    11221 aaccgcaaat ggttttatta agttcaatat ttataagtct ttcagggcaa ataagtgtgc
    11281 agatctctgt attagccaag tagtgaatat ggaggagttg cgctatgtgg caaattaatg
    11341 aggttgtgct atttgataat gatccgtatc gcattttggc tatagaggat ggccaagttg
    11401 tctggatgca aataagcgct gataaaggag ttccacaagc tagggctgag ttgttgctaa
    11461 tgcagtattt agatgaaggc cgcttagtta gaactgatga cccttatgta catcttgatt
    11521 tagaagagcc gtctgtagat tctgtcagct tccagaagcg cgaggaggat tatcgaaaaa
    11581 ttcttcctat tattaatagt aaggatcgtt tcgaccctaa agtcagaagc gaactcgttg
    11641 agcatgtggt ccaagaacat aaggttacta aggctacagt ttataagttg ttacgccgtt
    11701 actggcagcg tggtcaaacg cctaatgcat taattcctga ctacaaaaac agcggtgcac
    11761 caggggaaag acgttcagcg acaggaacag caaagattgg ccgagccaga gaatatggta
    11821 agggtgaagg aaccaaggta acgcccgaga ttgaacgcct ttttaggttg accatagaaa
    11881 agcacctgtt aaatcaaaaa ggtacaaaga ccaccgttgc ctatagacga tttgtggact
    11941 tgtttgctca gtattttcct cgcattcccc aagaggatta cccaacacta cgtcagtttc
    12001 gttattttta tgatcgagaa taccctaaag ctcagcgctt aaagtctaga gttaaagcag
    12061 gggtatataa aaaagacgta cgacccttaa gtagtacagc cacttctcag gcgttaggcc
    12121 ctgggagtcg ttatgagatt gatgccacga ttgctgatat ttatttagtg gatcatcatg
    12181 atcgccaaaa aatcatagga agaccaacgc tttacattgt gattgatgtg tttagtcgga
    12241 tgatcacggg cttttatatc ggctttgaaa atccgtctta tgtggtggcg atgcaggctt
    12301 ttgtaaatgc ttgctctgac aaaacggcca tttgtgccca gcatgatatt gagattagta
    12361 gctcagactg gccgtgtgta ggtttgccag atgtgttgct agcggaccgt ggcgaattaa
    12421 tgagtcatca ggtcgaagcc ttagtttcta gttttaatgt gcgagtggaa agtgctccac
    12481 ctagacgtgg cgatgctaaa ggcatagtgg aaagcacttt tagaacacta caagccgagt
    12541 ttaagtcctt tgcacctggc attgtagagg gcagtcggat caaaagccat ggtgaaacag
    12601 actataggtt agatgcatct ctgtcggtat ttgagttcac acaaattatt ttgcgtacga
    12661 tcttattcag aaataaccat ctggtgatgg ataaatacga tcgagatgct gattttccta
    12721 cagatttacc gtctattcct gtccagctat ggcaatgggg tatgcagcat cgtacaggta
    12781 gtttaagggc tgtggagcaa gagcagttgc gagtagcgtt actgcctcgc cgaaaggtct
    12841 ctatttcttc atttggcgtt aatttgtggg gtttgtatta ctcggggtca gagattctgc
    12901 gtgagggttg gttgcagcgg agcactgata tagctagacc tcaacattta gaagcggctt
    12961 atgacccagt gctggttgat acgatttatt tgtttccgca agttggcagc cgtgtatttt
    13021 ggcgctgtaa tctgacggaa cgtagtcggc agtttaaagg tctctcattt tgggaggttt
    13081 gggatataca agcacaagaa aaacacaata aagccaatgc gaagcaggat gagttaacta
    13141 aacgcaggga gcttgaggcg tttattcagc aaaccattca gaaagcgaat aagttaacgc
    13201 ccagtactac tgagcccaaa tcaacacgca ttaagcagat taaaactaat aaaaaagaag
    13261 ccgtgacctc ggagcgtaaa aaacgtgcgg agcatttgaa gccaagctct tcaggtgatg
    13321 aggctaaagt tattcctttc aacgcagtgg aagcggatga tcaagaagat tacagcctac
    13381 ccacatacgt gcctgaatta tttcaggatc caccagaaaa ggatgagtca tgagtgctac
    13441 ccggattcaa gcagtttatc gtgatacggg ggtagaggct tatcgtgata atccttttat
    13501 cgaggcctta ccaccattac aagagtcagt gaatagtgct gcatcactga aatcctcttt
    13561 acagcttact tcctctgact tgcaaaagtc ccgtgttatc agagctcata ccatttgtcg
    13621 tattccagat gactattttc agccattagg tacgcatttg ctactaagtg agcgtatttc
    13681 ggtcatgatt cgaggtggct acgtaggcag aaatcctaaa acaggagatt tacaaaagca
    13741 tttacaaaat ggttatgagc gtgttcaaac gggagagttg gagacatttc gctttgagga
    13801 ggcacgatct acggcacaaa gcttattgtt aattggttgt tctggtagtg ggaagacgac
    13861 ctctcttcat cgtattctag ccacgtatcc tcaggtgatt taccatcgtg aactcaatgt
    13921 agagcaggtg gtgtatttga aaatagactg ctcgcataat ggttcgctaa aagaaatctg
    13981 cttgaatttt ttcagagcgt tggatcgagc cttgggctcg aactatgagc gtcgttatgg
    14041 cttaaaacgt catggtatag aaaccatgtt ggctttgatg tcgcaaatag ccaatgcaca
    14101 tgctttaggg ttgttggtta ttgatgaaat tcagcattta agccgctctc gttcgggtgg
    14161 atctcaagag atgctgaact tttttgtgac gatggtgaat attattggcg taccagtgat
    14221 gttgattggt acccctaaag cacgagagat ttttgaggct gatttgcggt ctgcacgtag
    14281 aggggcaggg tttggagcta tattctggga tcctatacaa caaacgcaac gtggaaagcc
    14341 caatcaagag tggatcgctt ttacggataa tctttggcaa ttacagcttt tacaacgcaa
    14401 agatgcgctg ttatcggatg aggtccgtga tgtgtggtat gagctaagcc aaggagtgat
    14461 ggacattgta gtaaaacttt ttgtactcgc tcagctccgt gcgctagctt taggcaatga
    14521 gcgtattacc gctggtttat tgcggcaagt gtatcaagat gagttaaagc ctgtgcaccc
    14581 catgctagag gcattacgct cgggtatccc agaacgcatt gctcgttatt ctgatctagt
    14641 cgttcccgag attgataaac ggttaatcca acttcagcta gatatcgcag cgatacaaga
    14701 acaaacacca gaagaaaaag cccttcaaga gttagatacc gaagatcagc gtcatttata
    14761 tctgatgctg aaagaggatt acgattcaag cctgttaatt cccactatta aaaaagcgtt
    14821 tagccagaat ccaacgatga caagacaaaa gttactgcct cttgttttgc agtggttgat
    14881 ggaaggcgaa acggtagtgt cagaactaga aaagccctcc aagagtaaaa aggtttcggc
    14941 tataaaggta gtcaagccca gcgactggga tagcttgcct gatacggatt tacgttatat
    15001 ctattcacaa cgccaacctg aaaaaaccat gcatgaacgg ttaaaaggga aaggggtaat
    15061 agtggatatg gcgagcttat ttaaacaagc aggttagcca tgagaaactt tcctgttccg
    15121 tactcgaatg agctgattta tagcactatt gcacgggcag gcgtttatca agggattgtt
    15181 agtcctaagc agctgttgga tgaggtgtat ggcaaccgca aggtggtcgc taccttaggt
    15241 ctgccctcgc atttaggtgt gatagcaaga catctacatc aaacaggacg ttacgctgtt
    15301 cagcagctta tttatgagca taccttattc cctttatatg ctccgtttgt aggcaaggag
    15361 cgccgagacg aagctattcg gttaatggag taccaagcgc aaggtgcggt gcatttaatg
    15421 ctaggagtcg ctgcttctag agttaagagc gataaccgct ttagatactg ccctgattgc
    15481 gttgctcttc agctaaatag gtatggggaa gccttttggc aacgagattg gtatttgccc
    15541 gctttgccat attgtccaaa acacggtgct ttagtcttct ttgatagagc tgtagatgat
    15601 caccgacatc aattttgggc tttgggtcat actgagctgc tttcagacta ccccaaagac
    15661 tccctatctc aattaacagc actagctgct tatatagccc ctctgttaga tgctccacga
    15721 gcgcaagagc tttccccaag ccttgagcag tggacgctgt tttatcagcg cttagcgcag
    15781 gatctagggc taaccaaaag caagcacatt cgtcatgact tggtggcgga gagagtgagg
    15841 cagactttta gtgatgaggc actagagaaa ctggatttaa agttggcaga gaacaaggac
    15901 acgtgttggc tgaaaagtat attccgtaag catagaaaag cctttagtta tttacagcat
    15961 agtattgtgt ggcaagcctt attgccaaaa ctaacggtta tagaagcgct acagcaggca
    16021 agtgctctta ctgagcactc tataacgaca agacctgtta gccagtctgt gcaacctaac
    16081 tctgaagatt tatctgttaa gcataaagac tggcagcaac tagtgcataa ataccaagga
    16141 attaaggcgg caagacagtc tttagagggt ggggtgctat acgcttggct ttaccgacat
    16201 gacagggatt ggctagttca ctggaatcaa cagcatcaac aagagcgtct ggcacccgcc
    16261 cctagagttg attggaacca aagagatcga attgctgtac gacaactatt aagaatcata
    16321 aagcgtctag atagtagcct tgatcaccca agagcgacat cgagctggct gttaaagcaa
    16381 actcctaacg gaacctctct tgcaaaaaat ctacagaaac tgcctttggt agcgctttgc
    16441 ttaaagcgtt actcagagag tgtggaagat tatcaaatta gacggattag ccaagctttt
    16501 attaagctta aacaggaaga tgttgagctt aggcgctggc gattattaag aagtgcaacg
    16561 ttatctaaag agcggataac tgaggaagca caaagattct tggaaatggt ttatggggaa
    16621 gagtgagtgg ttaggctagc tacatttaat gacaatgtgc aggttgtaca tattggtcat
    16681 ttattccgta actcgggtca taaggagtgg cgtatttttg tttggtttaa tccaatgcaa
    16741 gaacggaaat ggactcgatt tactcatttg cctttattaa gtcgagctaa ggtggttaac
    16801 agtacaacaa agcaaataaa taaggcggat cgtgtgattg agtttgaagc atcggatctt
    16861 caacgagcca aaataatcga ttttcctaat ctctcgtcct ttgcttccgt acgcaacaag
    16921 gatggagcgc agagttcatt tatttacgaa gctgaaacac catatagcaa gactcgttat
    16981 cacatcccac agttagagct agctcggtca ttatttttaa ttaactccta tttctgtcga
    17041 agctgtttga gcagtaccgc tttacagcaa gagttcgacg ttcagtatga ggttgagcga
    17101 gatcatttag agataaggat cttacccagt tcatcgtttc ctaaaggggc gttagagctg
    17161 tcggccgtag tgcagctttt ggtttggttg ttttcggatc aagatgttat ggattcgtat
    17221 gaaagtattt ttaggcacta tcaacaaaat agagaaatta agaacggcgt tgaaagctgg
    17281 tgctttagct ttgaccctcc gcccatgcag ggttggaaat tacatgtaaa aggacgttct
    17341 tctaacgagg ataaggatta tttagttgag gaaatagtag gtttagaaat caacgctatg
    17401 cttcctagca caacagctat tagccatgcc tcttttcagg aaaaggaggc aggtgatggt
    17461 agtacgcagc acatagcggt ttcaacagag tcagttgttg atgatgagca tctacagttg
    17521 gacgatgagg aaacagccaa tatagacaca gacacacgag tcatagaggc tgagccgaca
    17581 tggataagtt ttagtagacc tagtcgaatt gaaaaatctc gcagggcaag aaaaagtagc
    17641 caaactattt tagaaaaaga agaagcaaca acaagtgaaa atagtaattt ggttagtact
    17701 gatgagccac acttaggtgg tgtcctagca gcggcagatg tgggtgggaa gcaggatgca
    17761 accaattaca actctatttt tgctaatcga tttgctgctt ttgatgagct actttcaatt
    17821 ctaaaaacta aatttgcatg tcgggtgctt tttgaagaaa ccttggtttt gccaaaagtt
    17881 gggcgtagcc gattacatct gtgtaaagat ggctcaccaa gagtgattaa agccgttggg
    17941 gtgcaacgta atggcagtga atttgtattg ctagaggtgg atgcatcgga tggggtgaaa
    18001 atgctttcta ccaaagtgtt gagtggcgtt gatagcgaaa catggcggaa tgattttgaa
    18061 aagatacggc gtggagtggt gaagagctca ttgaattggc caaatagttt gtttgatcaa
    18121 ttatatggac aagacgggca tagaggggtg aatcatccaa aggggttggg ggagctgcaa
    18181 gtatcgagag aggatatgga agggtgggct gagagagtgg ttagagagca atttacgcat
    18241 taaaggaatg actgaaagag cctgtaaacc cttttgtgta agtgcttttg ccggtcagtt
    18301 aaaggtggcc atttaaacgg tcaccaaatt cgatcataaa acggttcatg gccggcttcc
    18361 agttgcggat cggcatcgtc catttcttgg tcgccgcctg gatagccagg tacaccacct
    18421 tcatcgctga ttcgtccgta gggaacacct tgagttcata ttaatggaat tttctacaaa
    18481 tagcctccgt ggttttgagg ggggattaca gacgatccat agtagtaatc caatgagttc
    18541 ttgagcgcgg cgacatgttt ggacgccttg gcaaaaatta gagcctgctt gaagtgcagg
    18601 cgagcacgtg cttggacgat tgatccattc gcggtcaaaa actcaatctt ggatgacagc
    18661 gtgtcaggaa atccaatatc gaagtcccgc cccgtgtcaa agtagggcat ttcatgatca
    18721 aaggacgaag cttcccaggc cacttcccta atattccagc cgctagaaac aagatttcgt
    18781 acgacttcat caactttttt gctttccaac ttgtcacaaa gtgcataggc aagagctttg
    18841 gcggcagaca cgtgcgtttc ctcttaaaca taggtcgaac ggatacccga ggcaaagcgg
    18901 agcattagtc ccaaagcatc tccggtagat ccggaggctc catactgaag gcatggcatc
    18961 ttgggcattg atatttgaaa cttctctcta gatagcaggg atttcttggc cttgcctcat
    19021 gctgatagct tgtaaatatc acacgatcct tagccccaca aagtgagcat actccgtagc
    19081 tttcatttat agaaatcgtc gttagattgt cgcagcatgt gcacgttccg actatttgct
    19141 caggaagcat cgccatacct tggcctaggt aaagaagatc agactcgtag ccacatcggt
    19201 ggcaactgag cttgtacgca atgcccatct ggttgtctca cctgaatcgt aacgtcagag
    19261 ctattcatcc atccattcgg catcaatgta tgccactaag ccgaccagct gaccgtttgg
    19321 actgaagtat gttgcctgca ccccacttga catctcatca ataccaccgc actgcgctag
    19381 gcgccggtcc cacttgccca cagaagcgtc aggatcggct gagttcccca agacccttgt
    19441 gagccatgcc tcctttaact caacctctcg aatgattgcg tcggccagct cctccgcgtt
    19501 atctagttcg acatagtctg gaattcggac gtacgttctg aacacagctg gatacttact
    19561 tgggcgattg tcatacatga catcaacaat gtacccgttt gtgtaaccgt ctcttggagg
    19621 ttcgtatgac actagtggtt cccctcagct tgcgactaga tgttgaggcc taacatttta
    19681 ttagagagca ggctagttgc ttagatacat gatcttcagg ccgttatctg tcagggcaag
    19741 cgaaaattgg ccatttatga cgaccaatgc cccgcagaag ctcccatctt tgccgccata
    19801 gacgccgcgc cccccttttg gggtgtagaa catccttttg ccagatgtgg aaaagaagtt
    19861 cgttgtccca ttgttggcaa tgacgtagta gccggcgaaa gtgcgagacc catttgcgct
    19921 atatataagc ctacgatttc cgttgcgact attgtcgtaa ttggatgaac tattatcgta
    19981 gttgctctca gagttgtcgt aatttgatgg actattgtcg taattgctta tggagttgtc
    20041 gtagttgctt ggagaaatgt cgtagttgga tggggagtag tcatagggaa gacgagcttc
    20101 atccactaaa acaattggca ggtcagcaag tgcctgcccc gatgccatcg caagtacgag
    20161 gcttagaacc accttcaaca gatcgcgcat agtcttcccc agctctctaa cgcttgagtt
    20221 aagccgcgcc gcgaagcggc gtcggcttga acgaattgtt agacattatt tgccgactac
    20281 cttggtgatc tcgcctttca cgtagtgaac aaattcttcc aactgatctg cgcgcgaggc
    20341 caagcgatct tcttgtccaa gataagcctg cctagcttca agtatgacgg gctgatactg
    20401 ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt
    20461 tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca
    20521 gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc
    20581 aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct
    20641 tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc
    20701 aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca
    20761 cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc
    20821 tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc
    20881 aagccttacg gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc
    20941 cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac
    21001 gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatgatgtt
    21061 taactcctga attaagccgc gccgcgaagc ggtgtcggct tgaatgaatt gttaggcgtc
    21121 atcctgtgct cccgagaacc agtaccagta catcgctgtt tcgttcgaga cttgaggtct
    21181 agttttatac gtgaacaggt caatgccgcc gagagtaaag ccacattttg cgtacaaatt
    21241 gcaggcaggt acattgttcg tttgtgtctc taatcgtatg ccaaggagct gtctgcttag
    21301 tgcccacttt ttcgcaaatt cgatgagact gtgcgcgact cctttgcctc ggtgcgtgtg
    21361 cgacacaaca atgtgttcga tagaggctag atcgttccat gttgagttga gttcaatctt
    21421 cccgacaagc tcttggtcga tgaatgcgcc atagcaagca gagtcttcat cagagtcatc
    21481 atccgagatg taatccttcc ggtaggggct cacacttctg gtagatagtt caaagccttg
    21541 gtcggatagg tgcacatcga acacttcacg aacaatgaaa tggttctcag catccaatgt
    21601 ttccgccacc tgctcaggga tcaccgaaat cttcatatga cgcctaacgc ctggcacagc
    21661 ggatcgcaaa cctggcgcgg cttttggtac aaaaggcgtg acaggtttgc gaatccgttg
    21721 ctgccacttg ttaacccttt tgccagattt ggtaactata atttatgtta gaggcgaagt
    21781 cttgggtaaa aactggccta aaattgctgg ggatttcagg aaagtaaaca tcaccttccg
    21841 gctcgatgtc tattgtagat atatgtagtg tatctacttg atcgatcagg cttttgtata
    21901 tctccccacc acctgaaaca atgacatgat ccgttatttt ctttaggttg gttaaagcat
    21961 ctttaattga tggaaagatc aatacgttct cattgtcaga tgtaaaactt gaacgtgtta
    22021 cgaccgcata ctttcggttg ggtaatgctc ccattgattc aaaagtcttg cgtccaacca
    22081 acagccattg gttataggta atagctttaa acaggagctg ttcacctttg gcactccatg
    22141 gaatatcagg gccattcccg ataactccat tcttcgatat agctaccatt agtgatagtt
    22201 tcacaattct tcctcagagg ttaacatttt attagtaagc atgctcgttt tgtcacccgc
    22261 tgatgcttac cgttaattaa tataatcact tgtattaaat agactttctt taaaaataca
    22321 agacactctg ttattacaaa tcgtgcatgc cgtctatcct gcttgcacga tgcacattat
    22381 ctcacccaaa gaactcataa cattttaatt tatttagcat taaattaaaa ctctaccggt
    22441 aaacaagcat ctctaggcgt aaaaaacagg cactttttta aatcccagct agaataggct
    22501 gtataggcag acagttgcaa gacaaggagc gttttatgtc taacagtcca tttttaaatt
    22561 ctatacgcac ggatatgcga caaaaaggtt atgcgctgaa aactgaaaaa acttacctgc
    22621 actggattaa gcgttttatt ctgtttcaca aaaaacgtca tcctcagacc atgggcagtg
    22681 aagaggtcag gctgttttta tccagcttag caaacagcag acatgtagcc ataaacacgc
    22741 agaaaatcgc tttaaatgcc ctagcttttt tgtacaacag gtttttacaa cagccgttgg
    22801 gcgatattga ttatatccct gcaagcaagc ctagacggct accctctgtt atctctgcaa
    22861 atgaagtgca acgcattttg caggttatgg atactcgcaa ccaagttatt tttacgctgc
    22921 tgtatggtgc aggtttgcgc attaatgaat gcttgcgttt gcgggttaaa gattttgatt
    22981 ttgataatgg ctgcatcact gtgcatgacg gtaagggtgg gaaaagcaga aacagcctac
    23041 tgcccacgcg cctaatccca gcaataaaat aactcattga gcaagcgcgg cttattcagc
    23101 aagacgacaa cttacaaggc gtagggccat cgctgccttt tgctttagat cacaaatacc
    23161 cttctgctta tcgacaagcg gcgtggatgt ttgtctttcc ctccagcacg ctctgcaacc
    23221 acccgtataa cggcaaatta tgccgccatc atctgcatga ctccgttgcg cgaaaggcat
    23281 tgaaggcagc cgtacaaaaa gcaggcatcg ttagcaagcg tgtcacttgt catacatttc
    23341 gtcactcgtt tgctacgcat ctattacaag cggggcgtga tattcgcact gtgcaagaac
    23401 tcttagggca taacgatgtt aagaccacgc aaatctatac gcatgtgttg ggtcagcatt
    23461 ttgccggcac caccagtcct gcggatggac tgatgctact tatcaatcag taaaagtaga
    23521 taaatatctt tatgtagccc tgagtagccc ctgatacttt ttatggtgaa aaagataaac
    23581 aatcatagag gggataaata tgagccagtt ctaccattgt tcagataagt ttaagcaaga
    23641 tactgtcaat cacagtgacc attcatggtc attacacttc ctccagttgc cagacaatgc
    23701 ctaacataaa aacgcaaaag cccaaaagtc agagcttagg tgttctaggc gttttctaag
    23761 cgctgaagaa atacgtctta ccgtagattg cgtgcgtttc tgaagcacat tttccttata
    23821 catggcttta tcccaggcag cttcatcaag ctcttggagt aacaagccag taataatgcg
    23881 acactctcgg gtttgtagcg agccgctggt gagatcggag ttatagcgga aaggggtcat
    23941 ttgtaattat ttgtgggtgg aggatagtta acttattatt gtagttggaa gaggctgctt
    24001 tccaaacatt agcacctcgt aatgcttgtt actgtatatt aatttatgct cttagcgcat
    24061 gttaatgggc tattacaatg cataatgtcc acatcaagat aactgtggct taacacctgt
    24121 acaagcgaaa aaaatcaatc acctgtccgg aaatacggga tcactgtaaa gtgttggtga
    24181 ggcagtctag gagggggatt taatttggga tactgatgaa aggattatga tcagttggat
    24241 ataggatcgt actaagctga gcggcaggtt agctagatta gttaaccaga taagtgaaat
    24301 ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag
    24361 ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc
    24421 ccagttccca actattttgt ccgcccacag ctgccg
//
LOCUS       DAZSNM010000041        24444 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZSNM010000041 DAZSNM010000000
VERSION     DAZSNM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24444
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.041"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..202)
                     /locus_tag="ACHW42_001395"
     CDS             complement(<1..202)
                     /locus_tag="ACHW42_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR8943280.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLAPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGM"
     gene            complement(295..867)
                     /locus_tag="ACHW42_001396"
     CDS             complement(295..867)
                     /locus_tag="ACHW42_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR8943281.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            1085..1945
                     /gene="tesB"
                     /locus_tag="ACHW42_001397"
     CDS             1085..1945
                     /gene="tesB"
                     /locus_tag="ACHW42_001397"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR8943282.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1994..3280)
                     /gene="amtB"
                     /locus_tag="ACHW42_001398"
     CDS             complement(1994..3280)
                     /gene="amtB"
                     /locus_tag="ACHW42_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR8943283.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3310..3648)
                     /gene="glnK"
                     /locus_tag="ACHW42_001399"
     CDS             complement(3310..3648)
                     /gene="glnK"
                     /locus_tag="ACHW42_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR8943284.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3829..5610)
                     /locus_tag="ACHW42_001400"
     CDS             complement(3829..5610)
                     /locus_tag="ACHW42_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR8943285.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5603..7375)
                     /locus_tag="ACHW42_001401"
     CDS             complement(5603..7375)
                     /locus_tag="ACHW42_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR8943286.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7405..7863)
                     /gene="decR"
                     /locus_tag="ACHW42_001402"
     CDS             complement(7405..7863)
                     /gene="decR"
                     /locus_tag="ACHW42_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFR8943287.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(8017..8835)
                     /gene="cof"
                     /locus_tag="ACHW42_001403"
     CDS             complement(8017..8835)
                     /gene="cof"
                     /locus_tag="ACHW42_001403"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR8943288.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8935..10627
                     /locus_tag="ACHW42_001404"
                     /pseudo
     CDS             8935..10627
                     /locus_tag="ACHW42_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10692..11387
                     /gene="queC"
                     /locus_tag="ACHW42_001405"
     CDS             10692..11387
                     /gene="queC"
                     /locus_tag="ACHW42_001405"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR8943289.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11439..11837)
                     /gene="fadM"
                     /locus_tag="ACHW42_001406"
     CDS             complement(11439..11837)
                     /gene="fadM"
                     /locus_tag="ACHW42_001406"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR8943290.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11931..12302)
                     /locus_tag="ACHW42_001407"
     CDS             complement(11931..12302)
                     /locus_tag="ACHW42_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR8943291.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12453..14324)
                     /gene="ppiD"
                     /locus_tag="ACHW42_001408"
     CDS             complement(12453..14324)
                     /gene="ppiD"
                     /locus_tag="ACHW42_001408"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR8943292.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14516..14806)
                     /gene="hupB"
                     /locus_tag="ACHW42_001409"
     CDS             complement(14516..14806)
                     /gene="hupB"
                     /locus_tag="ACHW42_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR8943293.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14997..17351)
                     /gene="lon"
                     /locus_tag="ACHW42_001410"
     CDS             complement(14997..17351)
                     /gene="lon"
                     /locus_tag="ACHW42_001410"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR8943294.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17539..18813)
                     /gene="clpX"
                     /locus_tag="ACHW42_001411"
     CDS             complement(17539..18813)
                     /gene="clpX"
                     /locus_tag="ACHW42_001411"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR8943295.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18939..19562)
                     /gene="clpP"
                     /locus_tag="ACHW42_001412"
     CDS             complement(18939..19562)
                     /gene="clpP"
                     /locus_tag="ACHW42_001412"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR8943296.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19809..21107)
                     /gene="tig"
                     /locus_tag="ACHW42_001413"
     CDS             complement(19809..21107)
                     /gene="tig"
                     /locus_tag="ACHW42_001413"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR8943297.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21451..21768)
                     /gene="bolA"
                     /locus_tag="ACHW42_001414"
     CDS             complement(21451..21768)
                     /gene="bolA"
                     /locus_tag="ACHW42_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR8943298.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            22073..22651
                     /locus_tag="ACHW42_001415"
     CDS             22073..22651
                     /locus_tag="ACHW42_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR8943299.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22695..24170
                     /gene="ampG"
                     /locus_tag="ACHW42_001416"
     CDS             22695..24170
                     /gene="ampG"
                     /locus_tag="ACHW42_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR8943300.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5745 a         6729 c         5898 g         6072 t
ORIGIN      
        1 ccatgccaac aatacaccag accatcattt cgaggggaag acggcgcttg cgtagcgtta
       61 cagtacctga ttcggcaagg caacgagaga tgagttcggg ggcgaggtaa tcccccagag
      121 aagtcagtgg gttacgcaga gaatcgtaac gggataccag atcaagagcc tgtccaatgt
      181 gcataaaaaa atccggaaac aagtgagcgt ttccggattc ttacacagcc actggatcgg
      241 tcaactgatc cttaactgat cggcattact ccgggcggag agggttaacg tcgcttaata
      301 ttgcgtagga gcgggaactg ccgaagacgg attcacctga gttggtgaag tcgaaggtac
      361 ggtagtcgtt gcgccaccgc tggcctgaac cggcacggcg gtttgctgta ccggcaccaa
      421 tgtcaggtcg gctttagttc cgccctggtt gatcaccggc tgaacggtat cggtgataaa
      481 taccagtttg tcattcacgg taatcgccgc actcaacaga atacgcgcgt tcggctgaac
      541 atctgccggg ttaaatggca gaacaaagct gaatggtgac tgtttacctt cagtacgcac
      601 cgctttctgc gccagcactt ttgacggtgc atcggctaac gacgcgtcag aaagtgtcac
      661 ggtcagcaca gcatcaggcg gcagtgcgac tttctgacgg atccagacag taccggagac
      721 attcggttgc tggatagcag gttgttgtgt tgctgaaata gacgtatttg cagccggggc
      781 tggcgtctga atatccgcgc ttttatctgc gcaagccgcc aacgcaatcg caaccgctaa
      841 accactggcc atgtgcacga gtttcattca tttctcctta ttatcaatgc accagcgggc
      901 taactttcct cgccggaaga gtggttaaca aaatagtaac gtcaacaagt gtggcacaca
      961 tcacgcattt ctgcctgtaa ttagcccgta attcagacta tttcacccat cggaccagtg
     1021 gcaaatttgc gttatactca actcactttg gcttgctgcg gcagctttgt tactggagag
     1081 ttatatgagt caggcgctaa aaaatttact gacattgtta aatctggaaa aaattgagga
     1141 aggactcttt cgcggccaga gtgaagattt aggtttacgc caggtgtttg gcggccaggt
     1201 cgtgggtcag gccttgtatg ctgcaaaaga gaccgtcccg gaagagcggc tggtacattc
     1261 gtttcacagc tactttcttc gcccaggcga tagtaagaaa ccgattattt atgatgtcga
     1321 aacgctgcgt gacggtaaca gcttcagcgc ccgccgggtt gctgctattc aaaacggcaa
     1381 accgattttt tatatgacgg cctctttcca ggcaccagaa gcgggtttcg aacatcaaaa
     1441 aacaatgccg tccgcgccag cgcctgatgg cctgccttcg gaaacgcaaa tcgcccaatc
     1501 gctggcgcac ctgctgccgc cagtgcttaa agataaattc atctgcgatc gtccgctgga
     1561 agtccgtccg gtggagtttc ataacccact gaaaggtcac gtcgcagaac cacatcgtca
     1621 ggtgtggatc cgcgcaaatg gtagcgtgcc ggatgacctg cgcgttcatc agtatctgct
     1681 cggttacgct tctgatctta acttcctgcc ggtagctcta cagccgcact gcatcggttt
     1741 tctcgaaccg gggattcaga ttgccaccat tgaccattcc atgtggttcc atcgcccgtt
     1801 taatttgaat gaatggctgc tgtatagcgt ggagagcacc tcggcgtcca gcgcacgcgg
     1861 ctttgtgcgc ggtgagtttt atacccaaga cggcgtgctg gttgcctcga ccgttcagga
     1921 aggggtaatg cgtaatcaca attaaaaaaa tagccggagg tgaaaaccgt ccggctattt
     1981 tttgcagtgc ttgttacgcg ttataggcat tctcgccgtg gctgttgacg tccagccctt
     2041 ctcgctcttg ttcttccggc acacgtaggc caaccgtcag atccgccagt ttgtagccaa
     2101 taaatgccac cacaccggac cagacgatcg taatggcgat gctttccaac tgtaccagca
     2161 actgatggcc catcgtcaca ccttcagcga agcccacgcc gcccagagaa ctggcggcaa
     2221 aaatcccggt catgatacag ccgacaatgc cacaaacgcc gtgcacaccg aagacatcgc
     2281 agggatcatc cacccgcagc aagcgtttga gcatggtaac gccccacaaa cccgccagac
     2341 cagctaccac gccgataatc aacgcgccgc caaccccaat gtagccgcac gctggcgtca
     2401 cgccgaccag accggcaatc gcgccagaac acgctcccag cagagaaggc ttaccacgca
     2461 gcgcccattc accgaagatc cagccaagaa ttgccgccgc cgttgcgacc acagtattca
     2521 caaatgccag tgccgcgatt tcattcgccg tgcccactga cccggcgtta aagccaaacc
     2581 aaccgatata gagaatggca gtcccggtga agaccatcgg caggttgtgc ggtttaaacg
     2641 cctctttacc gaagcctacg cgtttgccta tcagatacgc gcccaccaga ccggcgattg
     2701 ctgcgttaat gtgcaccacc gtgccacccg cgaaatccag cgcaccgtga gaagccagca
     2761 aaccaccgcc ccacaccata tgcgcaatcg gaatgtaaga gagcgtcagc cataccacca
     2821 cgaaaatcaa cacagctgag aagcggattc gttccgccag cgccccaact atcaagccta
     2881 cggtaatgca ggcaaacgat ccctgaaacg ctacgtggat atactgataa atgctgccca
     2941 tcaccgccgt cagttcgatg ttttttagca tcaaccagtt aatgttgccg aagaagttgt
     3001 tgccctcacc aaacgccagc gaataaccgt aaaccaccca gagaatacag accagtgcaa
     3061 acgtcaccgt cacctgcgtc agcatcgaca gcacgttttt gccgcgaatc aacccaccgt
     3121 aaaacagggc aatccccgga atagtcataa acagcaccag cgcagtacaa atcatcataa
     3181 acgcattgtc ggctttatcg gccaccgcag gtgcagccat taccagtccc ggcagcatcg
     3241 ccagtgaagc aagcccagtt tttatcgtcg ctatcttcat ttttcgttcc tgttgctgtg
     3301 tgccagagat tacagcgccg cttcgtcggc ttcgccggta cgaatacgaa tgacgcgttg
     3361 caattcagcg acgaagattt tgccgtcgcc aatttttccg gtgtaagccg ccttactgac
     3421 gatatcgatc acttcatcga gttggtcatc agcaatcgcc acatcaattt ttacttttgg
     3481 caggaagttg acgctgtatt ccgccccccg gtacagctcg gcatgccctt tctgacgccc
     3541 gaaacctttc acttcggtga cggtcaggcc ctgaatacca atggaagata acgcttcacg
     3601 aacgtcttcc agcttgaatg gtttgattat cacggtcacc agcttcatag atcccctccg
     3661 gtcagaattc ggtaatggtt ctgctacacg aagaaggtat tgcaagcggt gtgccagaaa
     3721 tgaaaaacga gaaagagagc aggaagttaa gaagattgaa aatgaaaacg cactatgaca
     3781 gtgcacagtg cgttacattt ttgcaccaac gatgaaatat tgcgctattc aggcgctcaa
     3841 tgattcctct tcacgcacgc tggctgccag ctcttcgccc gcaagttgca gttgatacat
     3901 ctgccagtag cgtccctggg ccgccagcag ttgctggtga gtgccctgct ccacggcttg
     3961 cccacgatga agcaccagaa tggtgtcggc atcaataata gttgataagc ggtgagcaat
     4021 caccaccagc gtggtatgtt cacgcaccgc cgccagagca tgttgaatcg cctgttcagt
     4081 accggagtca atgctggcgg ttgcctcatc aaggatcagg atttgcggcg tctccaccag
     4141 cacgcgcgcc agtgccagca gttgcttttg cccgactgag agattatttc cctgctcgcc
     4201 cagcggcgta taaatacaat cactcatgct gcgcgccagc tccgccagtt gcacggtttc
     4261 cagcgcctgc cagacgcgtt cttcggagat atcccgcccc agcgtcacat tagcgaggaa
     4321 ggtatccgcc agcaccaccg gatcttgctg caccattgcc acgccctggc gcagcgcgct
     4381 atgacttagc gaacttaatg gacgaccatc aaggtgaatc tcaccttccg ttagcgggta
     4441 atagcccatc aataaactgg cgagggtact tttaccactg ccggtatgcc cgaccagcgc
     4501 cacaaagtta cgcgaaggca cagagagatt aatgttcttt agcaccagat tgtcatcgcg
     4561 ataagcaaat gacacgttat cgacttcgat ggtgccactc tgtaacgggt aatcatcatt
     4621 gccatattgc tggcgcggtc cgtccatcag ttcaaacacg cgctcaccag caacaacagc
     4681 ctgttgcagc atcgcctgtt gcgtggtcag ttcgattaag ggttcgttaa gtcgcccaag
     4741 atagctgata aacgcataca acacgcccac ttcaatggtg ccgctggcgg agaagccaaa
     4801 cagcatcaat aagccacaaa gaatgagcga tgaaaacaga ctcagcagcg gacgcagcag
     4861 aaaaccgtcg aggcgcaggg tttgcatcct agccatatag tgtgaacgac tggcctcccc
     4921 catacgttcg ccaaatcgcg cctgctgacg aaactgctgg ataacgctca tgccattgat
     4981 gatttcgtta aagccgtcgt tgatatccgc cagataggcg cgcacacggc ggacaatcgg
     5041 cgtgctgtaa cgctggtata tcaccattac caccagcacc accgggaaaa tcattatcgc
     5101 caccagcgcc attcgccagt cgaggctgaa catcgccacc agcatcgcgc ccaccagtgc
     5161 ggcactgcgc aggacagttg ccactacggt aacgtagaga tcgcggatca cttcagtgtc
     5221 attagtgacg cgggaaatca cctgcccgac gggttgggta tcaaactcgc ttaatggctg
     5281 gcgtaacgca gcatccatca cgtcggtacg caactgttgc actgcgccta ctgccgcccg
     5341 attaaacagc agcgactgcg cgtaatgtag cccggcggca aacagttgta gcccaacata
     5401 cgccgcagcc agccctgcaa ccactttcaa cggcaggtta tttttcgcta ccatattgtc
     5461 gataaaatag ctgataagca gcggcccact gacttctgcc gccgccgcaa cccacatcat
     5521 caggaccgca atccccagcg gtttacgcca cggcgaacca tacgctaaca ggcgcttgag
     5581 agtcggccac agttggctaa aactacgcat cgacggcctc ctcgcgaatt tccggaacgt
     5641 cgtcgagcgc cgcctccagt tgttgatagc gatacatatc gcgataccaa ccgctttgtt
     5701 gcgccagctc atcatgattg ccacgctggg cgatatgtcc gtgctgcatc acaataattt
     5761 cactggcttc cgtcagtgcc gaaaggcgat gggcactgat gattaccgtt cttccctgtc
     5821 cccactgacg caggttatgc aggatctggt gctctgtgcg tccgtccacc gccgaaagcg
     5881 catcatcaag gatgaggatt tccgcgttga ctaataacgc tcgagcaatg gagatacgct
     5941 gtttttgccc accggaaagc atcacgccgc gctcgcccac ttctgtatcg taaccttgcg
     6001 gtagacgcaa aatatcgtca tgtacgctgg ctaaccgcgc gacatgctca atctcttgct
     6061 gggtggcatt cgggcaaccc agcgcgatgt tattcgccac ggtgtcagaa aaaaggaatg
     6121 gcgtctggct gaccaccgcc aggcggctac gccagctatc gagttgtaac ttcgtcagag
     6181 gaatatcatg aaagcgaata tccccctcgc tgacgtcgaa atgacgctga atgagcgaca
     6241 acagggtact tttgccggaa ccagtcggcc cgcaaatacc cagcatctga ccgggtttca
     6301 gggcgaaatt gacgttttcc agcgcaggat ggtcagtctg cggatacgtg aactggtgaa
     6361 tatttacatc cagttcgcca cgcccttccg gcacgggttc gctaccatcg ttcaccaccg
     6421 gcgcttccgc cagcatggcg cgaatacggc tgtatgcagc actaccacgt tccacaatgt
     6481 taaacatcca tgccagcgcc agcattggcc aaatcatcag acctaaatac atcataaaac
     6541 tggtgagctg gcccagcgtt aaactgccct gcaccaccat ccaactaccg ccgccaatcg
     6601 ccagcaagtt cgccatacca atcgcgatat agatggtcgg gtcgaagcga gcatcaatac
     6661 gcgccacccg catgtttttt ttgccggtat cttcggcatc cgcggcaaat aacgccgact
     6721 ggcgatcttc cagaccaaag gctttgatca tgcggatact ggtgaggctt tcctgggtgc
     6781 ggtcattaag actggaaaac gccgcctgtg ccagcttaaa gcgttcatgc aaggcatcgc
     6841 cgttgcgctt aatcatgatc gccatcactg gcatcggtaa cagggcaaat aaggtcaact
     6901 gccagctaat ttgcgtagac atcataatca acacagcgca gcccattacc agtgaatcca
     6961 ccagcgtcag caccccttct ccggcggcaa acacgacgcg atcgacgtca ttggtcgcac
     7021 gagccatgag atcacccgtg cgatgacgca ggtaaaactc cgggtgctgc cggcttagct
     7081 gacggtaata atcttcacgc agttcaacag ccagttggta agacgcgcca aacagcaata
     7141 cgcgccagac ataacgcagg agataaacca caacggcaat cagcaccatg gtggcgatcc
     7201 acatcaggct cagcccggta gtaaagtgtt gttctgtcac gccatcgaca acaataccaa
     7261 ccacttttgg cggaaccagt tgcagcatcg cgataatgac aagcaaggcg acagccccaa
     7321 gatagcgacg ccattcccga cggaaatacc agcttaattg agcaaataat cgcacgcgtt
     7381 gtgtcctgac ctgattctgg atatttattc gatgggtaaa gaagtggtgt atttaatctg
     7441 ttccatcgcg aagctggaag tgacgtccga cagccccggc acgctgttta ccagacgctt
     7501 ataaaactcg tcgtagcgtt tcatgtcggc aacctggacg cgcatcagat aatcgtattc
     7561 accagccatg cgccagaccc ccagcacttc tggcatttcg gtaaccaccg tgacaaagcg
     7621 gcaataccat tcgctgctgt gatgttgcgt ttttatcagc acaaaggcgg tcaggccgag
     7681 gcctattttt tccggatcca gcagggcgac tttgccgata aggataccgt cgtcctccag
     7741 ccgtttcagg cgcttccagc agggggtggt tgtcagatta acggcttcag ccagtgcctg
     7801 caaagagagg gtgcaatcct gctgcagtaa ggccagcagc ttacggtcaa ttttatctaa
     7861 catagccctt ccacggagaa tttttttctc ttttaattct attttaaagg tgaaatcgcg
     7921 acaatttatt ctgtgcggtt taaacaccac ggcacaaaat gacaaattca acgcaaaaaa
     7981 aaaccgggca attgcccggt gctggaaggg atctcgttat tcgggggaat aaggtagatg
     8041 tggatagtcc agccagtgcg tcaaatagtg agagacagcc tgatttcggc aatgtccaat
     8101 caccggtaaa tgcgggagct ccgcgcgcag ttgcggcatc gcattgccca taataaaccc
     8161 gctaccgacg ctgcctaaca tttcgcgatc gttcatcgca tcaccaaagg ccatgcaatc
     8221 gcgcaacgat aaacctaaat gttgggtccg caccgtcaat gcagcgcctt tattgcagcc
     8281 caccggcagc acttcgaggc aatccgtggc ggaaaaacac aaatgtgcac gctcgcctaa
     8341 tgcttcgtat agctggatct gcaagcgtgt aagatcgtcg tgatcgccac agaagcagat
     8401 cttggtgacg ctgccgagtg gcattttttt gacatcgatt atctgataac gaaaaccgct
     8461 atagacaaat acctgcaaca acgcagggct ctctttcccg gtaaaccaac cgtcgtcatt
     8521 gaagatatgc atgctggctc gggtatccca ttgctgatac agcaccagct ccgcgacatc
     8581 cgcaggtaaa tcatcacgat gtaaaagttc accttccaga gaatgcacgc gcgttccgtt
     8641 gccggtaatc aaatatgcat ccagcgatag cgccccgaga atatgctgca tctccagcgc
     8701 atgacgcccc gtggcaaaag tgagggtaat gtcgcgttca cgcagtcgcg ccaaagtaga
     8761 gagggttttc tcacctaaat gatggtcagg catcaataaa gtgccatcca tatcaaatgc
     8821 taccagacga gccatttctt tctccactct gtgacacggt taatggtgat tgagtatcac
     8881 ctgatatata cggaagtaat agtgaatagt taaataatat tgttccgggt ttatatgcga
     8941 ttgctcaacc gtcttaacca gtatcaacgt ctgtggcaac cttcagccgc aaaccgtcac
     9001 cgtcagcgaa ctggccgaac gctgtttttg cagcgaacgc catgttcgta cgctgttgcg
     9061 tcaggcacag gaggcgggat ggctggagtg gcaggcgcag tcaggacgcg gaaagcgcgg
     9121 acaattacgc tttctggtca cgccggaatc gctacgcaat gcgatgatgg aacaggcact
     9181 ggaaaccgga aagcagcaag atgtgctgga gctggcgcaa ctggccccag gtgagctgcg
     9241 cactctgtta cagccattta tgggcggaca atggcaaaac gatacaccca cgttgcgtat
     9301 tccctactat cgcccgctcg aaccgctaca accaggcttt ttgcccggcc gtgccgagca
     9361 gcatctcgcc gggcagatat tttccggcct gacccgcttc gataataata ctcagcgccc
     9421 gattggcgat ttagcgcatc actgggaaac ctctactgac aggttacgct gggactttta
     9481 tcttcgttca accctacact ggcataacgg cgatgcagta aaagcctcac acttacacca
     9541 gcgattattg atgctgttac aactgccagc actggatcaa ttatttatta gcgtgaagcg
     9601 tattgaagtc acccatccgc agtgtctgac cttcttttta catcgccccg attactggct
     9661 tgcgcaccgg ctggcgagct attgcagcca tctggcgcat ccgcaattcc cactgatcgg
     9721 cactggtcct tttcgcttaa cacaattcac agcggagctg gtgcgcctgg agagccatga
     9781 ttattaccat ttgcgtcatc cgctgcttaa agcggttgag tactggataa ctccgccgct
     9841 tttcgaaaaa gatttgggaa ccagttgtcg gcatcccgtg caaatcacca tcggcaaacc
     9901 ggaggagctg caacgggtca gccaagtcag tagtggcatc agtttaggtt tttgctattt
     9961 aacgttgcgc aaaagtcccc gactctccct ctggcaggcg cgaaaagtga tctccattat
    10021 tcatcaatcc ggtttattac aaacgttaga agtcggagaa aacctgatca ccgccagtca
    10081 tgcattactg ccaggctgga ctattccgca ctggcaagta ccggatgaag tcaaactacc
    10141 gaaaaccttg acgctggttt atcacctacc gatagaactt cataccatgg cagaacgcct
    10201 acaggcgaca ctggcagcgg aaggctgtga actcacaatt atttttcata acgcaaaaaa
    10261 ctgggacgac acgaccctac tggcacacgc agacctcatg atgggcgaca gattaattgg
    10321 cgaagcaccg gaatatactc tggagcaatg gctacgttgc gatccactat ggccacatgt
    10381 tttcgacgct ccagcatatg cacatctgca atcgacgctg gacgcggtgc aagtaatgcc
    10441 tgatgaggaa aaccgattta atgccctgaa agcggttttt agccagttaa tggcagacgc
    10501 gacgctgacg ccgctgttca actatcacta tcgcattagt gcccctcccg gcgtgaacgg
    10561 tgtgcgactg acaccgcgcg gctggtttga atttaccgaa gcctggcttc ccgcgccgtc
    10621 gcaatgaata gctggtccgg gttaatcgca ggcgttacca taacgctttt attgttcaag
    10681 caggattatc tatgaaacgt gctgtcgttg tgttcagtgg aggccaggat tccaccacct
    10741 gtctggtgca ggcattacaa caatatgatg aagtccattg cgtgacgttc gattacggtc
    10801 agcgacatcg cgcagaaatc gacgtggcac gcgaactggc gctgaaactg ggagcacgcg
    10861 cgcataaggt tcttgatgta acgctgctca acgagctggc ggtcagcagc ctgacacgtg
    10921 acagcattcc ggtgcctgat tatgaacctg aagccgatgg catcccgaat acgtttgtcc
    10981 cagggcgtaa tattttgttc ctgacgctgg cggcaatata tgcgtatcag gtaaaagcag
    11041 aagccgtaat taccggcgtc tgtgaaacgg atttctccgg ctacccggat tgccgcgatg
    11101 agtttgtgaa agcactaaac catgccgtca gtttgggcat ggcgaaagat attcgttttg
    11161 aaacgccgct gatgtggatt gataaagcgg aaacctgggc gctggcagat tattacggca
    11221 aactggattt agtccgtaac gaaacgttga cctgctataa cggcattaaa ggcgacggtt
    11281 gcggtcattg tgcggcatgt aatttacgcg ccaacggttt gaatcattat ctggccgata
    11341 aaccgacggt gatggcagcg atgaagcaga aaaccgggtt gaggtaatta ttccgggtgt
    11401 cgccggatgc ggcttgagca tccggcacca caaaacgttt acttaaccat ctgctccagc
    11461 ttttcgcgca attccccttc cagagctaat gctttctgcg ttttaagatc aatacaaaca
    11521 aacgtaataa gcgcatccgc taccacctgc ccttccggct ccagtgtaat gacctggttt
    11581 aagatgccgc ttttaccgtt taattgctgc aactgactgg taatagttaa caggtcgctt
    11641 aataccgctg gacgacgata gttaatattg atattgacca cgacgaaggc gatgttatgg
    11701 gccgtcatcc actgaaaact gtcgctattt tccaccccat cccagcgggc ttcttcgaga
    11761 aattcaaggt agcgggcgtt gttgacgtgc tggtaaacgt cgagatgata tccacgaact
    11821 ttgatttgtg tttgcatagc gcaataacct tacgttgttg ttataagtac agaggtcata
    11881 actggtatga cctcttcagt ctggcaaaaa attgccacta tgcaaattaa ttacagggtt
    11941 aataccgcca gattacgttc caccagcgaa ttgcccatcc ccggcacctg ctttagatcc
    12001 tccaccgttt taaacggacc gtactcttcg cgataactga caatcgcctg cgctttcttc
    12061 aggccaacgc cattcatcgc gcgggctagc tcttccgcgc tggcattatt aatgctgacc
    12121 cgggtgcctt cttcgtcact ggctttcgcc ggtactgctg ctttactttg tgctgcagga
    12181 gcttccgctt tggtttctac cgccgtcggt ttcgccacag aagctgccgc cagcgcgcta
    12241 tgagacattc cggcacaggc cagggacagt gtaatgagca gtgctttaat tccgtgtttc
    12301 atactgtttt ctccttgttt gttaacagtg gaatcacctt aacgggagtg aaaacagtaa
    12361 acaaatggca atgttcagaa atggaaaagg ccgcgaaagc gaccttttct tgttacagct
    12421 gcaacgcatt tttttgcgag gcggcttcag gattattgct gttccagcgc atcgccaatt
    12481 ttgattttcg cctctttacg caggttactc atcagagctt caaagacgat ttgtgcgttg
    12541 ttctgggtga taccctgcac catcgctttt ttctgatctt ccggcattga accttgtttc
    12601 acttcatcca gcgccagcag aaccacatta ccttgcatat cggtcgccat accgtagctc
    12661 ggtttgtctt tcgctggcag tggcagtgca aacgccgcct ggctaatcgg gtcacgaccg
    12721 gaacggctta aggttttcgg ctcgccaaat ttcagaccgg cagcctgcat agcttccgca
    12781 cctttgccgg ctttcaaatc aaccagcagt ttctcagcat ccactttcgc ctgttgttca
    12841 gctttgttgt gctgaaccag tgccttaact tgttcctgaa catctgccaa cggtttcacc
    12901 gcttccggtt tgtgctcgct gacgcgcagc acgaatgcgc ggtcgccgtc tacggtgatg
    12961 atgtcagagt tgatgcccga cgcgccgttt tcacctacca gaccgccgtt aaagatagcg
    13021 tcggcaaccg gcttgaagtt caactcttcc ggcaggttat ctttgctgaa ccaacccgtc
    13081 tgagtggctt taacgccggc agcttgctct gcaccggcca gagactcggt gtcgttgctt
    13141 gccgcatcgc tcactttctg ctgcagcgca tagtacgcat cgagggcttt ttcgtgtttc
    13201 actttcgccg caatgtcgtc acgtacttcg tctaacgatt tcactttcgc tggctgaatg
    13261 tcgtccagac gtacaatcag gaaaccgacc gaagatttga tgacaccaga cagttggcct
    13321 ttttctttca gaccagcatt tttcagttcg tcggggatag tggcatcttc taaccaaccc
    13381 atatcgccgc cgttacgggc agagataata tcggcagatt tttctttggc taatgcagca
    13441 aaatcaccgc ctttattcag ctcatcaagt accgctttcg cttcatcttc agttttggtc
    13501 tggatgatgc tgtagcgggt acgctgcggc tgggtgaatt gatcctgatg ctgatcgtag
    13561 tagctctgga tatccgcatc gctaaccggt tgctgcatcg ttgcggcaac cagcttgatg
    13621 taactcacgc ggaattgttc cggcgtcatg aaattgtttt tgttttgttc gtagtagctg
    13681 gcaatctcct gttcggtcac aggctgcttc gccgccagcg cgttaacatc gatagtcgcc
    13741 tcacgcacca cgcgttgttg cgcgaccagt gccgccagct cgtcggtttc acctttcagc
    13801 ataaaatcgg taccggcaac gccgttaatc agctgttggg tagtgagctg gttacgcagc
    13861 gcctgggcgt actgatcggc ggtcatcccc atctggttga ggataccgtt atagcggctg
    13921 ttatcaaatt tgccgtcaac ctggaaggct ggggtcgcga aaatcgcctg tttaacctgc
    13981 tcatcactga tacccagttt cagctcacga gcgtactgat ccaacagcgc ctcgtcgatc
    14041 agacgattca gcacctgttg acgcagggtt ttcatatagc cttcgttcgc tgccagctcg
    14101 gagtattgat cgcccagctg ttgctgcata cgattacgct cgctgttaaa ggcgttctcg
    14161 aactgcccac ggctgatttc ctggtcattc acttttgcgg cgtagttatt gcctccgcca
    14221 atcaggtaac cactcacgcc ggtcaatatg aacgacacga taatgatacc gaaaataatc
    14281 ttgagcacga gactgtttgc agccgtgcgt aagctgtcca tcatggtgta acaacactcc
    14341 gctgtagatg actgtgtacc tcacgcaacg tatcgatgcg cgcaaaggcc tattgtgaca
    14401 agaaacgggg gcaattgtca gcccacaaga cacagaaagt caccgaatac aaataaaaaa
    14461 ggcacatcag tagatgcgcc cttgaacttc gtcacatccc cactggggac aacgcttagt
    14521 ttaccgcgtc tttcagtgct ttacctgcac ggaagctcgg tactttagcg gcagcgatgg
    14581 tgatctcttt accggtctgc gggttgcggc cagtacgggc agcacgctct ttaacggcaa
    14641 aagtaccaaa acctaccagt gctacatcat ccccttcttt cagagattca gttacggaag
    14701 caataatagc atctaacgca cggccagccg cagctttaga gatatcagcc cctgcagcaa
    14761 tcttgtcgat caattgagat ttattcactc ttctcttcct ctttataatt tatatcgcac
    14821 ttgaatcctt caaggtacga acgcgcagca gttatatcag gccagccatc cccttacaac
    14881 accccttaat aagggcaagc ccgatccgct atctaactta gcgagacaaa aaaaggctgg
    14941 caagcccgaa ttagcctgcc agccctgttt ttattagtgc attttgcgcg aggtcactat
    15001 tttgcagtca caacctgcat gccagacggt tcattttgca gcgccagagt cagaacttcc
    15061 tcaatgcgct tcacaggatg aatgtccaga tcggcaatta cgttgtcagg aatctcttcc
    15121 agatcgcgtt tattttcgaa cggaattagc actgttttaa tcccgccgcg atgcgctgcc
    15181 aggagttttt ctttcaaacc accgatcggc agtacctgac cacgcagagt gatctcaccg
    15241 gtcattgcca catcggcacg aaccgggtta ccggtcaggc aagaaaccag cgcggtgcac
    15301 atagcaatac cggcactcgg accatctttc ggcgtcgcac cttccggtac gtggacgtgg
    15361 atgtcacgtt tttcgtaaaa atcagggttg atccccagtt tttccgcacg cgcacgaacc
    15421 accgttaacg ccgcctgaat agactcctgc atcacttcgc cgagcgaacc ggtataggtc
    15481 agtttgcctt tacccggaac acatgcggtt tcaatggtca gcaagtcacc gcccacttcc
    15541 gtccacgcca gaccgattac ctgaccgaca cggttttcgt tatcagcgcg accatagtcg
    15601 aaacgctgaa caccgaggta gtcatgcagg ttatcgccgt taatttcgat atgttttaat
    15661 gacttatcga gcagtaactg cttaaccgct ttgcggcaca gtttggagat ttcacgctcc
    15721 agaccacgca cgcccgcctc acgggtgtag taacgaataa tgccgataat ggcgctatcg
    15781 tcgacggtca gctcaccttt tttcagtgca ttacgttcaa tctgcttcgg cagcaggtga
    15841 cgtttggcga tgttcagttt ttcatcttcg gtataaccgg agaggcgaat cacttccata
    15901 cgatccagca gcggtgccgg aatgttcatg gagttcgacg tcgcgacaaa catcacgtcg
    15961 ctgagatcgt aatccacttc caggtagtgg tcgctgaacg ctacgttctg ctctggatcc
    16021 agcacttcaa gcagtgcaga ggccggatcg cctcgcatgt cagaagacat tttgtcgatc
    16081 tcatcgagca ggaacagcgg atttttcacg cccactttcg ccattttctg gatcagttta
    16141 cccggcatag aaccgatgta agtacggcgg tgaccacgga tttccgcttc atcacgcacg
    16201 ccgcccagcg ccatacggac atatttacgc ccggtggctt tggcaatgga ctgaccaaga
    16261 gaggttttac ctacccccgg cggccctacc aggcagagga tcggtccctt gattttgttg
    16321 acacggcttt gaaccgcaag gtactcaagg atgcgatctt tcacgcgctc cagaccataa
    16381 tggtcggtat caaggatttc ctgcgcctga cgcaggtctt ttttgacctt gctgcgcgca
    16441 ttccacggta cctgtaccat ccagtcgata taaccacgca ctacggtcgc ttctgccgac
    16501 atcggagaca tcattttcag cttctgcaac tctgcttccg ctttctcttt tgcctctttc
    16561 ggcatcttcg ccgcgtcgat tttgcgcttc agggcttcgt tttcgtccgg cacgtcgtcc
    16621 atttcaccga gttctttctg aatagctttc atttgctcgt tcagatagta ctcacgctgg
    16681 gatttctcca tctgcttttt aacgcggttg cgaatgcgtt tctcaacctg cagcagatcg
    16741 atttccgatt ccatcattgc catcagatat tccagacgtt cgttaacgtc ggacatctcc
    16801 agaacggact gtttgtcagc cagtttcagc ggcatatgtg cagcaatggt atccgccaga
    16861 cgcgccggat cgtcgatgct attcagcgac gtcagcactt ctggtgggat ttttttgttc
    16921 agcttgatgt agccttcgaa ctggctgatt gcagtacgca ccagcacttc ctgttcccgc
    16981 tcatcaatgg tcggcgactc cagatactcc gccttcgcag aaaagtgttc gccattgtca
    17041 gagagcgcag aaatacgcgc gcgctgtaac ccctcgacca gcactttgac ggtgccgtca
    17101 ggcagtttca gcatctgcaa tatagaggcc acggtcccga cggtgaaaag atcgtttaca
    17161 cccggctcat ccgttgaagc ttctttctgc gcgaccagca taattttttt atcatggtcc
    17221 atcgccgctt ccagacaacg gatagatttt tcccgcccga caaataaggg gatgaccatg
    17281 tgcggataaa ccaccacatc gcgcagcggc aatacgggga tttcaatgcg ttcagaacgc
    17341 tcaggattca tagagctctc tcttagttta atttccgcca ggtaatcaga tgacacgact
    17401 gtgcttcacg ccatctatta acatgtacgt cagtatatgg ggatgtttcc cccacattca
    17461 acgccgagaa tagaggaaaa attaaagggg agataaaatc cccccttttt ggttaactaa
    17521 ttgtatggga atggttaatt attcaccaga tgcctgttgc gcttccggct taccataaat
    17581 cagcaacggt ttgctttgac catcaattac cgactcgtcg ataaccactt tttcgacgtc
    17641 ttccatggac ggcagatcgt acatggtatc gagcagtgcg gcttctacga tggaacgcag
    17701 gccacgggca ccggttttac gcgccatcgc tttcttagcg atagcatcca gcgcctcgtc
    17761 acggaattcc agatccacgc cttccagatt aaacagcgcc tgatactgct tggtcagggc
    17821 gtttttcggc tctttgagga tctgaatcag agcttcttcg ctcagttcat tcaacgttgc
    17881 tacaaccggc agacgaccaa tgaactcagg gataagacca aacttgatca gatcttccgg
    17941 ttcaacctgc gccagcagct cgccttcgct tgctttgtcg gacttcgctt ttaccgtcgc
    18001 gccaaaacca atgccggagc cggtttctac acggtgggaa atcactttat ccagaccggc
    18061 aaacgcaccg ccacaaataa acaggatctt agaggtatca acctgcaaga attcctgctg
    18121 cggatgttta cgcccacctt gcggtggaac agcagctacc gtaccttcga tcagtttcaa
    18181 cagtgcctgc tgtactcctt caccggaaac gtctcgggta atggacgggt tgtctgactt
    18241 acgagaaatc ttgtcgattt catcgatgta gacaatcccg cgctgcgctt tctgtacgtc
    18301 gtaatcgcac ttttgcaaca gcttctgaat gatgttttca acgtcttcgc ccacataacc
    18361 ggcttcggtc agcgtggttg cgtcggccat ggtgaacggg acgtccagca ggcgcgccag
    18421 cgtttcagcc agcagcgttt taccggaacc ggtcggaccg atcagcagaa tgttactttt
    18481 gcccaactcg acaccattgc tggtatcgcc gttgcgcaga cgtttgtaat ggttgtatac
    18541 cgcgaccgcc agcacttttt tcgcctgttc ctggccgata acgtaatcgt ccaggtggtt
    18601 gcgaatttca tgcggcgtcg gtagcgcact gcgttcacga tgcggtgcaa cttctttaat
    18661 ctcttcgcga atgatgtcgt tacataaatc aacacattcg tcgcagatat acacggatgg
    18721 accggcaatc agcttgcgca cttcatgctg gcttttgccg caaaaagagc aatacagcaa
    18781 tttgcctgag ccatctttgc gtttatctgt catgggtcaa aacctcttct ttgttctttg
    18841 tgccgcacac gacgacgcaa atgccattct caggcgcaag ccgcttacag cgttgtgccg
    18901 ccctagataa gtatagcggc acagttgcgc ctctggcatc aattacgatg ggtcagaatc
    18961 gaatcgacca gaccgtattc caccgcttca ggggcggaaa ggaagcgatc gcgctcggta
    19021 tcacgttcaa tctgttctaa tgattgaccc gtatgaagcg ccataagttc attcatgcgc
    19081 cctttaactt tcagaatttc acgggcatga atttcgatat cggtcgcctg gccctggtag
    19141 ccgcccaacg gttggtgaat catcacgcgc gaattcggca ggcaaaaacg tttacctttt
    19201 gcccctgcgg tcaacaagaa agcgcccatc gaggccgcct ggcccataca gatggtgctg
    19261 acatcaggct tgataaactg catggtgtca tagatagaca tcccggcagt gatcaccccg
    19321 cctggggagt taatgtacag atagatatct ttttctgggt tttccgcttc caggaacagc
    19381 atctgcgcca caatcaggtt agccatgtgg tcttcaacct ggccagtcag aaaaatgacg
    19441 cgttccttaa gtagacgaga atagatatca aaagagcgct caccgcgtga ggtctgttca
    19501 atgaccatcg gcaccagcgc catatggggt gcaaagttat ctcgttcgcc gctgtatgac
    19561 atttccgtct cctggataaa attgaaaaaa acctgctgta ccgattgtaa ccttatggac
    19621 ggattatcag ctagtccctg tgttatgggt acggcatcac catatttcaa gcataacaat
    19681 cttttgttgt tacgctaaca ccgaaagagg cttttcgcac ggctccatgc aaaattcatg
    19741 acaaaaaaaa gcccaccacc tggcggtgac gggcctttgt gcgaatttag cgcgttatgc
    19801 tgcgtaaatt acgcctgctg gttcatcagc tcgttgaaag tggtttcttt ttcagtcact
    19861 ttcgctttcg ccagtacagc ttcaacagcc tgttcttcca gagcaacatt gcgcatgttg
    19921 tccatcagtt ctttgttttt gctgtagaac tcgataactt ctttcggatc ttcgtacgca
    19981 gaagccatct cttcgatcag gcctttcacg cgctcttcgt cagctttcag ctcgttggtg
    20041 cggataactt cgcccagcag caggccaaca actacgcggc gtttagcctg ttcttcgaac
    20101 agttcgcgcg gcagttccag agcttgtttt tcgttgccac cgaaacgctg tgcagcctgg
    20161 cgacgcagaa cgtcgatttc gctgtcgatc agcgcagccg gtacgtcgat gtcgttagct
    20221 tttaccagac cttcgatcgc ctgagactta acgcggttac ggatggcgct cttcagctcg
    20281 cgctccatgt ttttacgcac ttcagcgcgc agaccttcta cggaaccatc ttcaacgccg
    20341 aaacgtttga tgaattctgc agtcagttcc ggcagttcac gctcttcaac tttcttcagg
    20401 ttgatagcga atttcgctgc tttacctttc aggttttctg cgtggtattc ttccgggaag
    20461 gtcacgtcga tggtgaactc ttcgccagct ttgtggcctt tgataccgtc ttcaaagccc
    20521 gggatcatac gaccctggcc catcgccagt acgaaatcag acgctttacc gccttcgaac
    20581 tcttcgccgt ctacagaacc ggtgaagtcg atggttacgc ggtcttctgc ttcaacagcg
    20641 ccgtcttttt ctttccaggt cgcctgctgt ttacgcagag tatccagcat gccgtcaacg
    20701 tcagcgtcgg tcacttcaac gatcggtttt tcaacttcga tcgcttccag accctgcagt
    20761 tcaacttccg gataaacttc aaactctaca gagtaagtga agtcttcacc cagcttgtat
    20821 tcgcccggaa cataagtcgg tgcgccagcc ggattgattt tttctttaat gatggcgtca
    20881 atgaagttac ggctcatcag gtcacccaga acgtcctggc gtacagacgc gccataacgc
    20941 tgagcaacga tattcattgg cactttgcct ttgcggaagc cgtcaatacg tacttttttc
    21001 gcaacgttga ccagctcgct tttaacagcg gtctcgatgc tgtcagcagc gatagtaatc
    21061 gttacacggc ggccaaggcc ttgagtggtt tcaactgaaa cttgcatctt gttacctcaa
    21121 aaaatcacag tgctcggtca actcttaccc cgcaagcaca ggttgttggc ttcgcggaac
    21181 cgggatgttc ttataatcaa tcacattccc tgttaccaga atcatcccga aagcattcaa
    21241 ataggacgcg gcattatagc ggcatcactt ttatgagtcg agaacggtta ttgcgcgctg
    21301 tcgcgtattt tttcacaatt cccgggcaat tttcgtctga atgctgggca aaaagcgcaa
    21361 aatccagaca aaacaaaacg gcccgcaggc cgtttttcat aaaatcttta gcaacatact
    21421 ggaaaagcgc cgacagttgc aaatgcgttt ttacgcgatg cttcctgctc cacgacaggg
    21481 aggagaggca aagacggtgt cctgcaaccc ttcccactcc ttaatagtgt aagtatgcag
    21541 agccagcgca tgaacggtag tagagagttc ctccgctaaa gtactgtaaa tcattcgatg
    21601 acgattcaga aaacgttcac ccgtaaaacg atcgctgacc agcacaactt taaaatggct
    21661 ttcagagccg gctgggacat tgtgacgata gctttcatcc actacttcga ggaatacggg
    21721 ttggaacgcc gcccttaatt tttcttctat ccgctcacgt atcatcatga aatatctcct
    21781 ccgacaacgc tgagatgtca tccatccctt taaatactag ccgcttttac cgtttccatt
    21841 gcagcttaac aacaaatatt taggtttcgt cctggttttt ccgtcaagcg tatgaagttt
    21901 actgatttaa agattgaaac acaggcctcg catcagcgtt tcttttacca ggggtgatta
    21961 tttcgtcaac atgatgaatt tacatgcctt acccacttcc cctcgccgtt ggcaatgtta
    22021 tgatggcggg aatttttacc aaacacgctt aagagtctct gagagcccga acatgttcaa
    22081 aaaaatcctc ttcccgttag ttgctctgtt tatgcttgca ggatgcgcaa aaccgccaac
    22141 aactattgaa gtttccccga cgattacact gccacagcag gatccaagcc tgatgggcgt
    22201 caccgtaagc attaatggtg ccgatcagcg taccgatcag gcgctggcaa aagtcacccg
    22261 cgataatcaa atcgttaccc tgaccgcctc ccgcgctctg cgtttcctgc tgcaagaagt
    22321 gctggaaaaa cagatgaccg cgcgtggtta catggttggt ccgaatggcc cggttaatct
    22381 gcaaatcatc gttagccaac tgtatgctga cgtgtcccag ggcaacgtgc gctacaacat
    22441 cgctaccaaa gcggatatcg ccatcatcgc taccgcgcag aatggcaaca aaatgaccaa
    22501 aaactatcgt gccagctaca acgttgaagg tgcgttccag gcctccaaca aaaatatcgc
    22561 cgatgcggtt aacagtgtgc tgaccgatac catcgctgat atgtctcagg acaccagcat
    22621 tcacgaattc atcaagcaga acgcgcgtta atttctgccc tctggcccgg tgcaagccgg
    22681 gcctgtagac gcccatgtcc agtcaatatt tacgtatttt tcaacagccg cgttcagcca
    22741 tattgctgat cctgggtttt gcttccgggc taccgctcgc cctgacatcc ggcaccttac
    22801 aggcctggat gacggtcgaa aatatcgatc tcaaaaccat tggtttcttc tctctggtag
    22861 gccaggctta cgtttttaaa ttcctctggt caccgctgat ggaccgctac acgcccccat
    22921 tttttgggcg gcggcgcggt tggctgctcg ccacgcaaat cctgttatta gtcgccattg
    22981 cggcgatggg ttttctcgaa ccaggcaccc aactccgctg gatggcggcg ctggcggtgg
    23041 tgatcgcttt ttgctctgcc tcccaggata ttgtcttcga tgcgtggaaa accgatgtgc
    23101 tcccggcaga agaacgtggt gcgggcgcgg caatcagcgt gctgggttac cgtttaggga
    23161 tgctggtttc cggcggccta gccctgtggc tggcagataa atggttgggc tggcagggca
    23221 tgtactggtt gatggcggcg ctgttgatcc cctgtattat cgcgacgttg cttgcaccag
    23281 aaccaaccga caccattcct gtgcccaaaa cgctggaaca agcggttgtt gcacctctgc
    23341 gagatttctt tggtcgcaat aatgcctggc ttattttgct tcttatcgtg ctgtataagc
    23401 tgggcgacgc attcgccatg agcctgacaa ccacgttttt gattcgcggc gtcgggtttg
    23461 atgcgggtga agtaggcgtg gtaaacaaaa cgcttggctt actggcgacc attgttggcg
    23521 cattgtacgg tgggattttg atgcagcgcc tgtcgctgtt ccgggcgctg ctgattttcg
    23581 gcattttaca aggtgcgtct aacgctggtt actggctgct gtcgattact gataagcatc
    23641 tctacagcat gggcgcagcc gtctttttcg aaaacctctg tggcgggatg ggcacatcag
    23701 cctttgtcgc gctgttaatg acgctgtgta ataagtcatt ttccgctact caatttgccc
    23761 tgctctcagc gctttccgct gtagggcgag tttatgtcgg ccccgtggcg ggttggtttg
    23821 ttgaagcaca cggctggtcg acattctatc tattctccgt cgccgctgcc gtaccggggc
    23881 ttattttgct gctggtttgc cgccagacgc ttgaatatac acgagtaaat gacaacttta
    23941 tctcccgtac cgaatatccg gcaggttatg cctttgccat gtggacactg gcggcgggcg
    24001 tcagcctgtt ggccgtgtgg ttacttctgt tgacgatgga cgcgctggat ttgacgcact
    24061 tctctttcct gcctgctctg ctggaagtcg gggttttagt cgccctttct ggcgtcgtgc
    24121 ttggtggttt gctggattat ctggcgctac gaaaaacgca tctgacgtaa tctgtaaata
    24181 ttatttagct gtagtaatca ctcgccgtaa ttattacggc taaataaaca tcagcatcgc
    24241 ttattaatat ttgttctttt gtgcggctta gcgtttggaa attattgacg ccatttataa
    24301 attctatttt tcttacacga ttcagctaat gagtctttat tttctcacca cccagttgtc
    24361 actttaatga taattatttg ttaaataatt gttttatttc gcattggtta taccaattgc
    24421 ccaccaagac tccttctgca ctcc
//
LOCUS       DAZSNM010000042        24397 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZSNM010000042 DAZSNM010000000
VERSION     DAZSNM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24397
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.042"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            225..1307
                     /locus_tag="ACHW42_001417"
     CDS             225..1307
                     /locus_tag="ACHW42_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR8943301.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(1796..2594)
                     /locus_tag="ACHW42_001418"
                     /pseudo
     CDS             complement(1796..2594)
                     /locus_tag="ACHW42_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(2604..3155)
                     /gene="ybcL"
                     /locus_tag="ACHW42_001419"
     CDS             complement(2604..3155)
                     /gene="ybcL"
                     /locus_tag="ACHW42_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR8943302.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(3324..3656)
                     /gene="emrE"
                     /locus_tag="ACHW42_001420"
     CDS             complement(3324..3656)
                     /gene="emrE"
                     /locus_tag="ACHW42_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR8943303.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(4001..4315)
                     /gene="fliE"
                     /locus_tag="ACHW42_001421"
     CDS             complement(4001..4315)
                     /gene="fliE"
                     /locus_tag="ACHW42_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR8943304.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            4529..6188
                     /gene="fliF"
                     /locus_tag="ACHW42_001422"
                     /pseudo
     CDS             4529..6188
                     /gene="fliF"
                     /locus_tag="ACHW42_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            6181..7176
                     /gene="fliG"
                     /locus_tag="ACHW42_001423"
     CDS             6181..7176
                     /gene="fliG"
                     /locus_tag="ACHW42_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR8943305.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            7169..7855
                     /gene="fliH"
                     /locus_tag="ACHW42_001424"
     CDS             7169..7855
                     /gene="fliH"
                     /locus_tag="ACHW42_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR8943306.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            7855..9228
                     /gene="fliI"
                     /locus_tag="ACHW42_001425"
     CDS             7855..9228
                     /gene="fliI"
                     /locus_tag="ACHW42_001425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR8943307.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            9250..9591
                     /gene="fliJ"
                     /locus_tag="ACHW42_001426"
                     /pseudo
     CDS             9250..9591
                     /gene="fliJ"
                     /locus_tag="ACHW42_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
     gene            9588..10715
                     /locus_tag="ACHW42_001427"
     CDS             9588..10715
                     /locus_tag="ACHW42_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR8943308.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            10820..11284
                     /gene="fliL"
                     /locus_tag="ACHW42_001428"
     CDS             10820..11284
                     /gene="fliL"
                     /locus_tag="ACHW42_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR8943309.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            11289..12293
                     /gene="fliM"
                     /locus_tag="ACHW42_001429"
     CDS             11289..12293
                     /gene="fliM"
                     /locus_tag="ACHW42_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR8943310.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            12290..12703
                     /gene="fliN"
                     /locus_tag="ACHW42_001430"
     CDS             12290..12703
                     /gene="fliN"
                     /locus_tag="ACHW42_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR8943311.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            12706..13071
                     /gene="fliO"
                     /locus_tag="ACHW42_001431"
     CDS             12706..13071
                     /gene="fliO"
                     /locus_tag="ACHW42_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR8943312.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            13071..13808
                     /gene="fliP"
                     /locus_tag="ACHW42_001432"
     CDS             13071..13808
                     /gene="fliP"
                     /locus_tag="ACHW42_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR8943313.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            13818..14087
                     /gene="fliQ"
                     /locus_tag="ACHW42_001433"
     CDS             13818..14087
                     /gene="fliQ"
                     /locus_tag="ACHW42_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR8943314.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            14096..14881
                     /gene="fliR"
                     /locus_tag="ACHW42_001434"
     CDS             14096..14881
                     /gene="fliR"
                     /locus_tag="ACHW42_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR8943315.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            15166..15789
                     /gene="rcsA"
                     /locus_tag="ACHW42_001435"
     CDS             15166..15789
                     /gene="rcsA"
                     /locus_tag="ACHW42_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR8943316.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(15833..16021)
                     /gene="dsrB"
                     /locus_tag="ACHW42_001436"
     CDS             complement(15833..16021)
                     /gene="dsrB"
                     /locus_tag="ACHW42_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR8943317.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            16184..16411
                     /gene="yodD"
                     /locus_tag="ACHW42_001437"
     CDS             16184..16411
                     /gene="yodD"
                     /locus_tag="ACHW42_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR8943318.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            16709..17524
                     /locus_tag="ACHW42_001438"
                     /pseudo
     CDS             16709..17524
                     /locus_tag="ACHW42_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(17521..19215)
                     /gene="dgcQ"
                     /locus_tag="ACHW42_001439"
                     /pseudo
     CDS             complement(17521..19215)
                     /gene="dgcQ"
                     /locus_tag="ACHW42_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(19386..19568)
                     /locus_tag="ACHW42_001440"
     CDS             complement(19386..19568)
                     /locus_tag="ACHW42_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR8943319.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(19647..20564)
                     /locus_tag="ACHW42_001441"
     CDS             complement(19647..20564)
                     /locus_tag="ACHW42_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR8943320.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            20737..21657
                     /gene="yedA"
                     /locus_tag="ACHW42_001442"
     CDS             20737..21657
                     /gene="yedA"
                     /locus_tag="ACHW42_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFR8943321.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(21646..22116)
                     /gene="vsr"
                     /locus_tag="ACHW42_001443"
     CDS             complement(21646..22116)
                     /gene="vsr"
                     /locus_tag="ACHW42_001443"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR8943322.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(22097..23515)
                     /gene="dcm"
                     /locus_tag="ACHW42_001444"
     CDS             complement(22097..23515)
                     /gene="dcm"
                     /locus_tag="ACHW42_001444"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR8943323.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(23582..24083)
                     /locus_tag="ACHW42_001445"
                     /pseudo
     CDS             complement(23582..24083)
                     /locus_tag="ACHW42_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(24162..>24397)
                     /gene="drpB"
                     /locus_tag="ACHW42_001446"
     CDS             complement(24162..>24397)
                     /gene="drpB"
                     /locus_tag="ACHW42_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR8943324.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6123 a         6275 c         6055 g         5944 t
ORIGIN      
        1 ataaataaca acctttaata taatttgact accaaattta tattcgttca caaaatagtc
       61 atgcattgtg tgcaatagaa acagttcaga taaaaatagg gatctacttc acaatttaaa
      121 tgagaaacta aaacttacat cttgaaataa tctcattgat tggatgaata tttgtagtgc
      181 agtgacatca tttttaaata atagttcaaa taaaagggct cacgatgaaa aaattaacag
      241 tggcaatttc tgctgtagct gcatcagtac tgatggcgat gtctgctcag gcagcagaga
      301 tttacaataa agacagtaac aagctggatc tgtacggaaa agttaatgct aagcactact
      361 tctcttctaa cgatgcagat gatggcgaca caacttatgt tcgtctgggc ttcaaaggcg
      421 aaactcaaat caacgatcaa ctgactggtt tcggtcagtg ggaatatgaa ttcaaaggca
      481 accgtgctga atctcaaggt tcttccaaag ataaaacccg tcttgccttc gctggcctga
      541 aattcggtga ctacggctcc atcgattatg gccgtaacta cggtgtagca tacgatatcg
      601 gtgcatggac cgacgttctg ccagaattcg gtggcgatac ctggacccaa acagatgtgt
      661 tcatgactgg tcgcaccaca ggtgttgcaa cctatcgtaa caacgacttc tttggtctgg
      721 ttgatggtct gaactttgct gctcagtatc agggtaaaaa tgaccgcact gacgtaactg
      781 aagctaacgg tgatggtttc ggtttctcca ctacttatga gtatgaagga ttcggtgtag
      841 gtgcaaccta tgctaaatct gaccgcacta atgatcaggt tatctacggt aacaacagcc
      901 tgaatgcatc tggtcaaaat gctgaagtat gggcagctgg tctgaaatat gatgcgaaca
      961 acatctatct ggctaccacc tattctgaaa cccagaacat gactgttttt ggtaataacc
     1021 atattgccaa caaagcacaa aacttcgaag tagttgcaca atatcagttc gacttcggtc
     1081 tgcgtccgtc cgttgcttac ctgcaatcta aaggaaaaga cttgggtgcg tggggtgatc
     1141 aggacctgat tgaatatatt gatgtaggtg caacctatta cttcaacaaa aatatgtcca
     1201 cttttgttga ttacaaaatc aacctgattg ataagagcga tttcacgaaa gcatctggcg
     1261 ttgctaccga tgatatcgtt gctgtaggat tggtttacca gttctaatct gattactcaa
     1321 agatatgttg tgggaggctt tgcctcccca acatataagt ggcgctctca agccactttc
     1381 cttagaagca ctatcttgct tctgactata taaattaccc tttattttct gggacgtttc
     1441 aacgccccat ttttaataat actcagtgaa caattaacgt atcaattaga tttaataaca
     1501 acgatatccg tacctaacct gatatctaat gtcataagca tccctttaat tatcccctca
     1561 acctcttgta aatttcccac tcttgagctc cacatagccc aaacgccgga tgcaaaaaaa
     1621 atcatacgtg catctctgaa attgtgatgg aggaagtgag tatcaattat atgtgcttat
     1681 aaacgttccc gattggcaca gaagtcaccg gattgttcag gctccaatag gttgagtatg
     1741 tcgtatttat tttccaaaat aaaaatcgat gctgttgctc cttaataaaa tacttttact
     1801 ctctggacgc ttttcttagc tctcttggtg ttatattaaa acgattatgg aacctttcag
     1861 taaaacgaga aggacacttg taaccatttt ccctggcaat ctcgctgatg ggttttactg
     1921 tcgtttgtat agcaggacaa cgcattattt aacctcacgt cgtccaatat actttgaaaa
     1981 cttattccct cgcttgccag acggcgatgc aatgtggaaa cagaaatgta gagatatctc
     2041 gctaccttgt ttgctgtcca ttttgttcta ggttcggata gcattaggtt ataacaacga
     2101 cttattaatg attgtttact atctgataaa agtaaacaat taacatgatg tgctcctaat
     2161 gaaagtagaa cgcccattgc taagtgttcc tgaatttgcg ttgagaagcc tcgggaaata
     2221 gatgttttta gttgctccca acaatatatt aactcggggt tctgaggtaa gcagaaacct
     2281 gtcgtgttac gaatttgatc agttatcgca taaaattttt gaaacttttc aattagatca
     2341 atgggataat aaagcatttc tgcaagataa agacctgctt ctggataatt cacaatataa
     2401 aattcatatc cacagggaaa taatattatc tgattattat caacagttag actatgcgtc
     2461 tcccaattga taactttttt tccctgacgg atacgacata aagctggcat aagaggctta
     2521 accctgtgaa tctcatgatg tttatgcatc cgtatttctt caatctttag gttagactta
     2581 tctcttgcca gcatactctt accctatttt atctcataaa ctggtgttat ctcagcggtt
     2641 gcaattttat tggcattaag catataacca actaacgctc cgctggaatt agaatccaca
     2701 ggaatcttat cagtttttag agcccatact ttaaactggt aatgatgggg tttatctcct
     2761 ttgggaggac atgctccacc aaatcctgca taaccaaaat catttcggcc ttgaacagca
     2821 ccagtcggaa gttttgtacc atcacgtctt cctgcatcag tgggcaaata tgttacagtt
     2881 gctggaatat tagcaacagt ccagtgccac catccactgc ctgtaggagc atctggatca
     2941 tacacagtta cggcaaagct tttggtgcct tcaggagcac cagaccaggt taatgagggc
     3001 gatgtattac caccttcaca cccaaatcca gaaaatacat gagacgttgt aagttgctct
     3061 cctgttttta tttcattact agtgacctga aatgctgcgg cctgcgcaga aaatgttatg
     3121 aatgccaata cacttgaaac gataagtttt ttcataaaaa cctcattgtt gtgacctatc
     3181 gttattttat ttgatgtgcc ttgatctcat tatgcataaa ggcgcaatga tcgcgcaaat
     3241 gcaatcataa ttataaaggt gccccgaaca ttagccataa tatgcacaaa tcagattcct
     3301 gttatctcaa ggagaattat tttttaatgc ggtgcacttc gtgacaacaa gttaatgact
     3361 aacacaccgg cacaaatcaa catcatgcct atgatagctg gcaggtccag tcgttgacca
     3421 aaaaatcccc aggaaagcaa actaatcaga acgataccga ctcctgacca gatagcataa
     3481 gcaatccctg taggtatata agccagcgtc tgagctaata accagaatga tgcacaatag
     3541 caaataattg tgccaacaga gggccataac cgtgtaaacc cctctgaaaa cttcattaag
     3601 gttgtaccaa tgacctctgc aagtattgcg ccaccaagat aaatataagg gttcatagca
     3661 tattctttcc tgttcatact ggagagaatt gtactacagt ttgaacacaa ctcaccgttt
     3721 tcatcctggt gtatgtgtat ccaaggggct ctggcgtggc aagagataaa ggcaggaaca
     3781 ataatgatca gaaggaaccc caggctaacg aatgagttca ctcttcctgg gagcaaagcg
     3841 aagacaaaac atgttatcgc gtttacggat cgtggatggc agaaaacaac caggatcagg
     3901 tatccatttt aaaccagaaa ttgagtgagt ttgccccatc catgccccac gctgtgggat
     3961 cggatataat taaacaagca tagtcaacag gttacacaac ctacacctgc atggacatca
     4021 cttcctgata cgccgccacc agcttattac gcacctgaat ccccatttgc atagaaactg
     4081 aggctttttg catatcggtc atcacatcgt ttagcgccac gccgggttca ccgagggtga
     4141 atttttctgc ctgcgtgcgg gcgactgttt gtgtatcgct tatgcgatcg agcgcggcgt
     4201 gcagctgccc ggcaaaacta atggtcggtt gcggcagtga ttcctgcgca cgcgcactca
     4261 tcgccgtagc ctgtaactgg ctgataaccc cttcaatccc ctgtatcgct gacattttcg
     4321 tctcccggat aatttctggt aacaaagcct accagtaagt caataagaca aaggcgctaa
     4381 atagcaacaa aaaaactggt ttattggcgg atagaaaaaa cgaaagcaca aataatggga
     4441 gcgtcaattt ttcgagtgtg ctgacccgag agtgagtctt gttccacttt gccaataacg
     4501 ccgtccataa tcagccacga agtgcgcgat gaatgcgact gcagcccaga caaaatctct
     4561 tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca ttgattgtta ccggttccgc
     4621 ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa gcccccgact accgcacatt
     4681 attcagcaat ctttccgatc aggatggtgg cgcaattgtc agccaactga cgcaaatgaa
     4741 tattccttac cgcttcagcg aagccagcgg cgctattgag gttccggcag ataaagttca
     4801 cgaactgcgc ctggcgcaac aaggtttgcc caaaggcggc gcggtcggtt tcgaactgct
     4861 cgatcaggaa aagtttggta tcagccagtt cagcgaacag gtgaattatc agcgggcgct
     4921 ggaaggcgag ctttctcgta ccatcgaaac tatcggcccg gtaaaagggg cgcgcgtaca
     4981 tctggcaatg ccgaaaccgt ctttattcgt ccgtgaacaa aaatcccctt ctgcatcggt
     5041 gacggtaaat ctgttacccg gccgcgcact cgatgaaggg caaattagcg ctattgtaca
     5101 tctggtttcc agcgcggttg ctggtctgcc gcctggaaac gtcacgctgg tggatcaggg
     5161 cgggcatctg ttaacccagt ccaataccag cgggcgcgat cttaatgacg ctcagttgaa
     5221 atatgccagc gatgtcgaag gccgtattca gcagcgtatt gaagcgatcc tgtcacctat
     5281 tgttggtaac ggtaatattc acgcctaggt cacggcgcaa ctggacttcg ccagtaaaga
     5341 acaaacggaa gaacagtatc gccctaacgg tgatgaatct catgcggcgc ttcgttcacg
     5401 ccagcttaat gagagcgagc aaagcggttc cggttatccg ggcggcgtac cgggggcgtt
     5461 gtcgaatcaa ccggcacctg cgaataacgc gccaatcagc acgcctccga caaatcaaaa
     5521 taaccgccag cagcaggcga gcaccaccag caatagtggg ccgcgtagca cacagcggaa
     5581 tgaaaccagt aactacgaag tcgatcgcac cattcgtcat accaaaatga acgtgggcga
     5641 tgtgcaacgt ctgtcagtcg cggtagtggt gaattacaaa accttgccag acggtaaacc
     5701 attgcctctc agcaacgaac agatgaagca aattgaagct ctgacccgcg aggcgatggg
     5761 cttttctgaa aaacgcggtg attcgctcaa tgttgttaac tcgccgttca atagcagtga
     5821 cgaaagcggc ggagcactgc cgttttggca acagcaagtg tttatcgatc agttgctggc
     5881 tgccggtcgc tggttgctgg ttgctggtac tgctggtggc gtggttgctg tggcggaaag
     5941 cggtacgccc gcagctaaca cgtcgcgccg aggcggtgaa gactgtgcag caacaggcgc
     6001 aggcccgcga ggaagtggaa gatgcggtgg aagtccgtct gagcaaagac gaacaactcc
     6061 aacaacggcg cgctaaccaa cgactggggg cagaagtcat gagccagcgt atccgtgaaa
     6121 tgtctgataa cgatccgcgc gtggtggcgc tggtcattcg ccagtggata aataacgatc
     6181 atgagtaacc tgacaggcac cgataaaagc gtcatcctgc tgatgaccat tggcgaagac
     6241 cgggcggcag aggtgttcaa gcacctctcc cagcgcgaag tgcaaaccct gagcgctgca
     6301 atggcgaacg tcacgcagat ctccaacaag cagctaaccg atgtgctggc ggagtttgag
     6361 caagaagctg aacagtttgc tgcactgaat atcaacgcca acgattatct gcgttcggta
     6421 ttggtcaaag ctctgggtga agaacgtgcc gccagcctgc tggaagatat tctcgaaacc
     6481 cgcgataccg ccagcggtat tgaaacgctc aactttatgg agccgcagag cgccgccgat
     6541 ctgattcgcg atgagcatcc gcaaattatc gccaccattc tggtgcatct gaggcgcgcc
     6601 caggccgccg atattctggc gttgttcgat gaacgtctgc gccacgacgt gatgttgcgt
     6661 atcgccacct ttggcggcgt gcagccagcc gcgctggccg aactgaccga agtactgaat
     6721 ggcttgctcg acggtcagaa tctcaagcgc agcaaaatgg gcggcgtgag aacggcagcc
     6781 gaaattatca acctgatgaa aacgcagcag gaagaagccg ttattaccgc cgtgcgtgaa
     6841 ttcgacggcg agctggcgca gaaaatcatc gacgagatgt tcctgttcga gaatctggtg
     6901 gatgtcgatg atcgcagcat tcagcgtctg ttgcaggaag tggattccga atcgctgctg
     6961 atcgcgctga aaggggccga gcagccactg cgcgagaaat tcctgcgcaa tatgtcgcag
     7021 cgtgccgccg atattctgcg cgacgatctc gccaagcgtg gtccggtgcg tctgtcgcag
     7081 gtcgaaaacg aacagaaagc gattctgctg attgtgcgcc gtcttgccga aactggcgag
     7141 atggtgattg gcagcggcga ggatacctat gtctgataat ctgccgtgga aaacctggac
     7201 gccggacgat ctcgcgccac caccggcaga gtttgtgcca atggtcgagt cggaagaaac
     7261 cattattgaa gaggccgaac ccagccttga gcagcaactg gcgcaactgc aaatgcaggc
     7321 ccacgagcag ggttatcagg cggggattgc cgaaggtcgc cagcaaggtc acgaacaggg
     7381 ctatcaggaa ggactggccc aggggctgga acaaggtctg gcagaggcga aggctcaaca
     7441 agcgccaatt catgcccgga tgcagcaact ggtcagcgaa tttcaaacta cccttgatgc
     7501 gcttgatagt gtgatagctt cgcgcctgat gcagatggcg ctggaggcag cacgtcaggt
     7561 gatcggtcag acaccaacgg tggataactc ggcactgatc aaacagatcc aacagttgtt
     7621 gcagcaagaa ccgttattca gcgggaaacc acagctgcgc gtacacccgg atgatctgca
     7681 acgtgtggat gatatgctcg gcgcaacttt aagtttgcat ggctggcgct tgcggggcga
     7741 tcccaccctc caccctggcg gatgtaaagt ctccgccgat gaaggcgatc tcgacgccag
     7801 tgtcgccact cgctggcaag aactctgccg tctggcagca ccaggagtgg tgtaatgacc
     7861 acgcgcctga ctcgctggct aaccacgctg gataactttg aagccaaaat ggcgcagttg
     7921 cctgcggtac gtcgctacgg gcgattaacc cgcgctaccg ggctggtgct ggaagccacc
     7981 ggattacaat tgccgctcgg cgcaacctgt gtcattgagc gccagaacgg cagcgaaacg
     8041 cacgaagtag aaagcgaagt cgttggcttt aacggtcaac ggctgttttt aatgccgctg
     8101 gaggaagtgg aaggtgtcct gcccggcgcg cgtgtatatg ccaaaaacat ttcggcagaa
     8161 gggctgcaaa gcggcaagca gttgccgctc ggtccggcgt tattaggtcg tgttctggac
     8221 ggtagcggta aaccgctcga tggcctgccc ttccccgata cgacggaaac cggtgcgctg
     8281 attaccccgc catttaaccc gttgcaacgt acaccgattg aacatgtgct ggacaccggc
     8341 gtgcgcccaa tcaatgccct gcttaccgtc ggacgcgggc agcgtatggg gctgtttgcc
     8401 gggtcgggcg ttggtaaaag tgtgctgctg gggatgatgg cacgctacac ccgcgccgat
     8461 gtcattgtcg tgggtttgat tggtgaacgt gggcgcgaag taaaagattt tattgagaat
     8521 atcctcggtg ccgaaggacg tgcacgctca gtggtgattg ccgctccggc ggatgtttct
     8581 ccgctcctgc gaatgcaagg tgccgcctat gccacgcgca ttgccgaaga ttttcgcgat
     8641 cgtggtcagc atgtgttgct gattatggac tccctcaccc gctacgcgat ggcccagcgt
     8701 gaaattgcgc tggcgattgg cgaaccacct gccactaaag gttatccacc gtcggtgttt
     8761 gccaaattac cggcactggt cgagcgtgcc ggtaatggca ttagcggcgg cggctcgatt
     8821 accgcgtttt ataccgtgct caccgaaggc gatgaccagc aggaccccat tgctgactcc
     8881 gcgcgggcaa tcctcgacgg tcacattgtg ctgtctcgcc gactggcgga agccgggcac
     8941 tatccggcta tcgatattga agcgtcgatc agtcgcgcaa tgacggcgtt gatcagtgag
     9001 caacattacg cgcgagtgcg caccttcaaa cagctgttgt cgagttttca gcgtaaccgc
     9061 gatctggtta gcgtcggcgc gtatgccaaa ggcagcgatc cgatgctcga taaagccatc
     9121 gccctgtggc cgcagctgga gggctatttg caacaaggca tttttgaacg cgcggactgg
     9181 gaagcgtctc tccaggggct ggagcgtatt ttcccgacag tgtcataacc caggagataa
     9241 cggcagatgg cggaagaaca gctcaaaatg ttgatcgatt atcagaatga atatcgcaat
     9301 aacctcaata gcgatatgag tgccgggatg accagcaacc gctggatcaa ctatcagcag
     9361 tttatccaga cgctggaaaa agccattact cagcatcgcc agcaacttaa tcagtggacg
     9421 cagaaagttg acattgccct gaacagctgg cgagaaaaga aacaacgttt gcaggcctgg
     9481 cagacactgc aggaacggca atccacggcg gcactgcttg cagaaaaccg cctcgatcag
     9541 aaaaagatgg atgagttcgc ccagcgcgcc gccatgagga aacctgaatg attcgcttag
     9601 cgcccttgat taccgccgac gttgacacca ccacattgcc tggcggcaaa gccagcgatg
     9661 ctgcacaaga ttttctcacg ttgttgagcg aagcattagc aggcgagaca acaactgaca
     9721 aagcggcccc ccagttgctg gtagcaacag ataagcccac gacaaaaggc gagccgctgg
     9781 tcagcgatat tcttgccgat gcgcaacaag cggatttact gatccctgtg gatgaaacac
     9841 ttcctgtcat caacgacgaa caatccacat caacaccgtt aaccaccgct cagacgatga
     9901 cgttggctgc ggtggctgac aaaaatacga caaaagacga aaaagcggat gatcttaatg
     9961 aagacgtcac cgcaagtctg agcgcccttt ttgcgatgtt gccgggtttt gacaatacgc
    10021 ccaaagtgac tgatgcaccg tcaaccgtgt taccggcaga gaaaccaacg ctcttcacaa
    10081 aactgacttc tgcgcaactc actacagcac agcctgatga cgcccccggc acaccagctc
    10141 agccattaac accgctggta gcagaagccc agagtaaagc ggaagtcatc agcacgcctt
    10201 cgccggtgac cgctgacgcc agcccgctaa tcactccaca ccagacacag ccactgccca
    10261 ccgtcgccgc gcctgttttg agtgcaccgc tgggttctca cgaatggcaa caatcattga
    10321 gccagcatat ttcgctgttc acccgccagg ggcaacaaag tgcagagttg cgtctgcacc
    10381 cacaggattt aggtgaagtg caaatttccc tcaaagtgga tgataaccag gcgcagatcc
    10441 agatgatttc accgcatcag cacgtacgcg ccgccctgga agcagcgctg ccggtactgc
    10501 gtacgcagct ggccgaaagt ggcattcagt tagggcaaag caacattagt ggcgaaagct
    10561 ttagtggtca gcagcaggcc gcttcccagc aacagcaaag ccaacgcaca gcaaaccatg
    10621 aacctctggc gggggaagac gacgatacgc ttccggttcc cgtctcttta caagggcgtg
    10681 taacaggtaa cagcggcgtt gatattttcg cctaacgtca gaggtagcac cgtaatccgc
    10741 gtcttttccc cgctttgttg cgctcaagac gcaggataat tagccgataa gcagtagcga
    10801 cacaggaaga ccgcaacaca tgactgatta cgcgataagc aagaaaagca agcgatcgct
    10861 ttggatcccg attctggtat tcattaccct cgcggcctgt gccagcgcag gttacagcta
    10921 ctggcattcg catcaggttg ccgctgacga caaagcgcag caacgcgtcg tgccctcccc
    10981 ggtcttctat gcgctggata cctttacggt caatttgggc gatgcggatc gcgtgcttta
    11041 tatcggcata accctgcgac tgaaagatga agcaacccgc tcgcggctga gtgagtattt
    11101 gccggaagtc cgtagtcgct tgctgttact gttttcacgt caggatgctg ccgtactggc
    11161 gacagaagaa ggcaagaaaa acctgattgc cgagattaaa accacacttt ccaccccgct
    11221 tgttgccggg caaccgaaac aggatgtcac cgacgtgctg tataccgctt ttattctgcg
    11281 ataacgacat gggcgatagt attctttctc aagctgaaat tgatgcgctg ttgaatggtg
    11341 acagcgaagt caaagacgaa ccgacagcca gtattagcgg cgaaagtgac attcgtccgt
    11401 acgatccgaa tacccaacga cgggttgtgc gcgaacgttt gcaggcgctg gaaatcatta
    11461 atgagcgctt tgcccgccat tttcgtatgg ggctgttcaa cctgctgcgc cgtagcccgg
    11521 acattaccgt cggggccatc cgcattcagc cgtaccatga atttgcccgc aacctgccgg
    11581 tgccgaccaa cctgaaccta atccacctga aaccgctacg cggcacaggg ctggtagtgt
    11641 tctcgccgag tctggtgttt atcgccgtgg ataacctgtt tggcggcgat ggacgcttcc
    11701 cgaccaaagt ggaaggtcgc gagtttaccc atactgaaca gcgcgtcatc aaccgtatgt
    11761 tgaagctggc gctggaaggc tatagcgacg cctggaaagc gattaatccg ctggaagttg
    11821 agtacgtgcg ttcggaaatg caggtggaat ttaccaatat caccacctcg ccgaacgaca
    11881 ttgtggttaa cacgccgttc catgtggaga ttggcaacct gaccggcgaa tttaatatct
    11941 gcctgccatt cagcatgatc gagccgctac gggaattgtt ggttaacccg ccgctggaaa
    12001 actcgcgtaa tgaagatcag aactggcgcg ataacctggt gcgccaggtg cagcattcac
    12061 agctggaact ggtggccaac tttgccgata tctcgctacg cctgtcgcag attttaaaac
    12121 tgaaacccgg cgacgtcctg ccgatagaaa aacccgatcg catcatcgcc catgttgacg
    12181 gcgtcccggt tctgaccagt cagtacggta ctctcaacgg tcagtatgcg ttacggatag
    12241 aacatttgat taacccgatt ttaaattctc tgaacgagga acagcccaaa tgagtgacat
    12301 gaataatccg gccgatgaca acaacggcgc aatggacgat ctgtgggctg aagcgttgag
    12361 cgagcaaaaa tcaaccagcg aaaaaagcgc tgccgacgcg gtgttccagc aatttggcgg
    12421 tggtgatgtc agcggaacgt tgcaggatat cgacctgatt atggatattc cggtcaagct
    12481 gaccgtcgag ctgggccgta cgcggatgac catcaaagag ctgttgcgtc tgacgcaagg
    12541 gtcagtcgtg gcgctggacg gtctggcggg cgaaccgctg gatattctga tcaacggtta
    12601 tttaatcgcc cagggcgaag tggtggttgt tgccgataaa tatggcgtgc ggatcaccga
    12661 tatcattacc ccgtccgagc ggatgcgccg cctgagccgt tagtgatgaa taaccatgct
    12721 actgtgcaat cttccgcgcc ggtttctgct gagccactgc tgcaggtgag cggcgcactc
    12781 atcgccatta ttgccctgat cctcgctgct gcctggctgg taaaacggct gggatttgcc
    12841 cctaaacgca ctggcgttaa cggtctgaaa attagcgcca gtgcttcgct gggcgcgcgt
    12901 gaacgggtcg tggtggtcga tgtggaagat gcacggctgg tgctcggcgt taccgcaggg
    12961 caaatcaatc tgctgcataa acttccccct tcagcaccaa cggaagagat accgcagacc
    13021 gattttcagt cggtcatgaa aaatttgctt aagcgtggcg ggagatcctg atgcgtcgtt
    13081 tgttttctgt cgcaccggtc cttctctggc tgattacgcc cctcgccttc gcgcaactgc
    13141 cgggtatcac cagccagccg ctacctggcg gtggacaaag ctggtcgctc ccggtgcaga
    13201 cgctggtgtt catcacctcg ttgacgttca ttccagcgat tttactgatg atgaccagtt
    13261 tcacccgcat cattattgtt tttggtttat tgcgtaacgc gctgggaacg ccctccgcgc
    13321 cacctaacca ggtattgctg gggctggcac tgtttttgac cttttttatt atgtcgccag
    13381 tgatcgacaa aatttatgtc gatgcgtacc agccattcag cgaagagaaa atctcaatgc
    13441 aggaggcgct ggaaaaaggg gcgcagccgc tgcgtgagtt tatgctgcgt cagacccgcg
    13501 aggcagattt agggttgttt gccagactgg cgaataccgg cccgttgcag gggcctgaag
    13561 ctgtgccgat gcgcattttg ctcccggcct acgtgaccag cgagttgaaa accgcatttc
    13621 agataggctt cacgattttc atcccttttt tgattatcga cctggtgatt gccagcgtgt
    13681 tgatggcatt ggggatgatg atggttcccc ccgccaccat tgctctgccc tttaaactga
    13741 tgctgtttgt actggtggat ggctggcaat tgctggtcgg ttcgctggcg cagagctttt
    13801 acagctagag aggcaaaatg acacctgaat cggtcatgat gatgggaact gaagcgatga
    13861 aagtcgcgct ggcactggca gccccgctgt tgctggtagc gctggtcacg ggccttatca
    13921 tcagtatttt gcaggcagcc acgcagatta acgaaatgac gctgtcgttt attccgaaaa
    13981 tcatcgccgt atttatcgcc atcattattg ccggaccgtg gatgctcaat ctgttgctgg
    14041 attacgtccg caccttgttc actaacctgc cgtatatcat cgggtagccc gtacaatgat
    14101 gcaggagaca agcgatcaat ggctttcctg gttaagcctg tacttctggc cgttactgcg
    14161 cgtgctggcg ctcatttcta ccgcgccaat tctgagcgaa cgcagcgtgc cgaaacgggt
    14221 aaaactgggt ctggcaatga tgatcacgtt cgccattgcc ccatcattac ctgccaacga
    14281 tgttcctgtt ttttcgttct ttgctctgtg gctggccgta cagcagatcc tgatcggcat
    14341 tgcgcttggt tttaccatgc aatttgcctt tgccgctgtg cgaaccgctg gcgaaattat
    14401 cggtctgcaa atggggctgt catttgcgac atttgtcgat cctggcagcc atctcaatat
    14461 gcccgtttta gcgcgcatca tggatatgct ggcattactg ctgttcctga catttaacgg
    14521 tcatttatgg ttgatttcac tgctggtcga tacctttcac accctgccga ttggtagcga
    14581 accgttgaac agcaatgcgt ttctggcacc caccaaagca gggagtttga ttttccttaa
    14641 cgggctgatg ctggcgttac cgctcattac tctgttgctg accctgaatc tggcattagg
    14701 tttactgaat cgtatggccc cgcaattatc catttttgtt attggatttc cattaactct
    14761 gactgtcggc atctctttaa tggcggcatt aatgccgtta attgcgcctt tttgcgaaca
    14821 tttattcagt gaaattttta atttgctggc tgatattatt agtgaattac cattaatata
    14881 attccgtaac gtttatcatg ctaccctaag gattatccga aaaataatat ctgcgaacat
    14941 cttccaggat actcctgtag cgaaatattt gttttaagct cactcacata tcgcaacatt
    15001 tactttaaga caattccagg caaattatac aacactttac gggatagtaa gtccgcctga
    15061 aaaattgcga gagtggcgca ttaggtgacc catgttgttc cgtttagtca tgatgaaata
    15121 ttcaggtaag gggaattatc gttacgcatt gagtgagggt atgccatgtc aacgattatt
    15181 atggatttat gtagttacac ccgactaggt ttaaccgggt atctgttgag tagaggggtt
    15241 aaaaaaagag aaatcaacga cattgaaacc gttgatgacc ttgccatagc ttgtgattca
    15301 cagcgtcctt cagtggtgtt tattaatgag gactgtttca tccacgatgc ttctaacagt
    15361 cagcgcatca agcacatcat taatcaacat cccaatacgt tatttatcgt ttttatggca
    15421 attgccaatg ttcattttga tgaatatcta ttggtcagaa aaaatttatt gatcagttct
    15481 aaatcgatta aaccggaatc tctcgacgat atccttggcg atattctgaa aaaagagaca
    15541 acgataacct cgtttttaaa tatgccgacg ttatcattga gccgaaccga atcgagtatg
    15601 ttgcgaatgt ggatggcagg tcagggaacc attcaaatct ccgaccaaat gaatatcaaa
    15661 gccaagaccg tttcatcgca taaaggtaat attaaacgta agatcaaaac gcataataaa
    15721 caggttatct accatgtcgt ccgactgacg gataatgtga ctaatggtat ttttgtcaac
    15781 atgcgctaac acattctgac tggtggtttc ccaccagtca ggctgaataa gattactctg
    15841 ctttctcaac aaagataccg tcctgatgcc ctgcttcatt aaagaaccag atgccgagcg
    15901 ggtagtcttc cagcgcaacc aggtacattg tgccttcact aaactcctca actgccagta
    15961 ccactccagg acgacgcgga ccgccatccg ttttgactgt tacccgatca ttcaccttca
    16021 tcgttttcct cctgtggctt tgtgccagtg tagaacaatt tcgtttttct ggcagcgcca
    16081 agcgcgcgcg agtgctgatt ttctcgacgg tctatactta agagatgcca ggcggactta
    16141 acgactggcg gcaacaacag agtaacggtt gcgaggaaag atgatgaaaa ccgcaaaagt
    16201 gtacagcgat accgcaaaac gtgaggtcag cgtcgatgtc gatgccctgc tggcggcgat
    16261 caatgaaatt agcgaaagcg aagttcatcg cagccagaac gattctgaac acgttagcgt
    16321 cgatggacgt gaatatcata catggcgtga attggcggat gccttcgaac tggatattca
    16381 tgacttcagc gtctctgaag tgaatcgttg aatgcacaat aaaaaaatcc cgaccctgag
    16441 ggtgtcggga tgaaacttgc ttaagcaaga agcacttaaa aaattcgtta caccaggaaa
    16501 tctgatgtgt tcatcacctt atctgcaatt tttttcgctg acaagaaaat attcgcgcta
    16561 tgaatgatta acgctactta tgaatagaaa tgtgacgcgc gtcacccctt ggtggctctg
    16621 gtgtcaacgg gatcgctccc gaaaaaatca tctttgatta tttgtgctgt tttgagaatc
    16681 atcctggtaa ggggagtact gcgcgaccat gttttcaatt caacaaccac tactggtttt
    16741 tagcgatctt gatggcaccc tgctggacag tcatagttat gactggcaac cggcagcccc
    16801 ctggctcagc cgtttacacg aagccaatat tcccgtcatt ctctgtagca gtaaaacttt
    16861 agcggaaatg ctgtacttgc aaaaaatgtt ggggctacaa ggcttaccgc tgattgcaga
    16921 gaatggcgca gtgatccagc ttgctgaaca atggcaggat atagacggtt ttccacgcat
    16981 catctcaggt attagccatg gcgaaatctg ccaggtttta aatacgctac gcgagaaaga
    17041 acattttaaa ttcacgactt ttgatgatgt cgacgatgca accattgccg aatggacggg
    17101 attaagccgt agctaggcgg cgctgacgca gctgcatgag gcgtcggtaa cgctaatctg
    17161 gcgcgacagt gacgagcgta tggcacaatt tatcgctcgt ctgaacgaac tgggcttgca
    17221 gtttatgcag ggtgcgcgct tctggcacgt actggatgcc tctgccggaa aagatcaggc
    17281 tgccaactgg attatcgcga cctatcaaca attgtcaggc agacgcccaa ccacacttgg
    17341 cctgggcgat gggccaaacg atgcgccttt actggaggta atggattacg cggtgattgt
    17401 gaaagggcta aaccgtgaag gggtacatct gcatgatgag gatccggccc gcgtctggcg
    17461 aacgcagcgt gaaggaccgg aaggctggcg tgaagggctg gaccattttt tctccgcccg
    17521 ttaagcgtta tcgctcgcga atacccgatt gcgcccggcc tgttttgcga ggtaaagccg
    17581 acggtcggcc aatgactgga gttgttcaaa atcataatca ccggtttcct cgctgctact
    17641 tacccccagc gaggcactga tgcgtatcgt tgtactcttg gcgatcagca tctctttttc
    17701 atttaacttc aggcgaatac gttctgcgac ctccgcagcc tgcgtcagat tcgcgcctgg
    17761 cagaatcaca caaaactcct caccaccgac ccgcccggca acgtcctgcg cacgcaagga
    17821 actgctaatt aatccggcag catgagaaag aacacggtcg cccgcctgat gaccaaagcg
    17881 gtcattaatc gctttaaaat ggtcaagatc gacctgaatg acagaaaaag gatgttggtg
    17941 cgtctgacac aatttagcga gcggacgggc tttttcgaac agtgcgccac ggttatataa
    18001 acgcgttaag gtgtcgtgcc acgcctgcca ctgcaacgag ctttgcagaa catacatgct
    18061 gctgaccatc cggcgaatca cataccagga gaggagtaac atggtggtaa agagcgccca
    18121 cagcagggtt aatgcaatgc tgatactgcc gaaatcgccg cgcacgcctt cgcttagcgt
    18181 atggacgcgc gccagcacac cgtcgaaatg gtccagacgt tcccagctaa catagcgact
    18241 gtccatacga atgccgcccc gcgtgtcatg ttccattgcc tgcgccagca aggccagttc
    18301 acgaggatca aaaatattcc ctgttggatg gtcaggattg gaagaggtca aaaatctcag
    18361 cttactgtca tagagctgat actcaccatc gaggttttta tcgatggcgt ttcttaaaaa
    18421 ttgctgcata gtacgcacgg gaatactcat ccccagcacg ccataccagt agttattact
    18481 gtctaccgga acactgacgg taacctgcgg ttcagtattg ctggcgtgtt ccggttgcga
    18541 agtgaaccag cgtaccgcgc ggtgacgatt ttctcgttgc gaatggccga taaaccaggg
    18601 ttgggtgaca tagccgtaat aacgcgttgg tacattgcgc gtaaacaagg tcggctgcgt
    18661 cgaaacgtaa aatccagcac gcgagacata catcgcctgt tcaaccatcg acgaggagtt
    18721 gtgcgccagt cgcagcaagt aaccaacttc cagtgcagcg gtaatttcat tgtcgaggct
    18781 ttcattttcg cgagacagga aattcccctc gctgactaat gcatccgata cgccattgac
    18841 tggcagggtg cgccgtcggt tgagttcgat ttgccaggcg tgctcgtcgc gatgctgttc
    18901 gaactcggtt acggcattac gcagagaggt gaaatccagt ggcgccacga gagcttcgcg
    18961 catgccatta cgcaaaaaga tcagtttgtc gacgttatac tgcaaatgct tatcgagcgc
    19021 gttggctacg ttttccagat gattacgcta gctggagata taggcatctt ccagcaccac
    19081 cacttcacgc caggtgagca aggtggaaaa aagcaatacc acgataaagc agagattaac
    19141 gacatgaccg ggccccaggc ggcgtgcgag ttttttcaac cagctctggt tttccatttt
    19201 tgtctcgtgc tgcaccctga cgactcccaa cctgcttttt tatgattctg gcataacccg
    19261 gcaatagcgt accgcaaaac agcgataatt ttataatggt ggcatgataa gacgcgacta
    19321 tcattcattg caggcattgg gacaccaggc cggatgtgat ccggcctggt gttaatgata
    19381 ccctttcaaa cttcttccgc agaacgactc ccctcccccg gaacaagatc ggtttcatga
    19441 aaagcttccc gcttgacccc gtaaccgtca taccaacgac actcaaccat accgctggag
    19501 tatccagtga caatcatccg cgggccgccc tctttaaccg taacttcctc actaaccata
    19561 aagctcatac tcgcctcctt ttttcgagtg aaacttgttc accttagttg aagatggcga
    19621 attttgcata atagccagtg cgagtattaa tgtgcctgac cgcgcatttt cgctactgct
    19681 ttcactccca gcaccacgat gccgccgatg ataaatccaa gaatcagatt taaaacagtc
    19741 ggtaatatca tcgccaccac tgcactttgc tgcccggcga tatgttcaat agcgtgatgc
    19801 agcggcgcaa taccatgtac cacaatcccg ccgccgacga ggaacatcgc cagcgtgccg
    19861 acaatcgata atgctttcat cagccaaggc gcgataatca ataacccttt acctaatgcc
    19921 tgcatcagcg cgctagattt ttccgccagc caatacccta ggtcatcaat cttaacgata
    19981 acccctacca gaccgtagac gcccacggtc actaccagcg cgatgcctga aagcaccagc
    20041 acctgattaa gcaacggcgc ctctgccaca atccccagcg tgatggcgac gatttccgca
    20101 gacaaaataa aatcggtacg aatcgcccct ttaattttgt ccttttcaaa cttcagcgga
    20161 tcctgcgccg ccagcttctc cagacgctgc tggctctgcg ctggatcttc tttatgttta
    20221 cgcgcctcca gcatatgcag cactttctct accccttcaa agcagagaaa cgcgccacca
    20281 atcatcaaca gtggcgtaat cgcccacggg ataaacgcac tgatgatcag cgccagcggc
    20341 accagaatca ctttattgat cagcgatcct ttcgccacgc cccagaccac gggaagttcc
    20401 cggttggccc gcacgcctga aacttgttgc gcattgagcg ataaatcatc ccctaataca
    20461 ccggcggttt tcttcgccgc cagtttgccc atcacagaga tatcgtccag cagtgtggcg
    20521 atatcatcga gcaacgtcag taaactactc ccggccaaaa gcattccctc tcatttttat
    20581 tgttgaaggg tgtaagtatg aagcaaaaac ggcagctctg atacttgctt cacaggtcaa
    20641 catttattta acaatcaagt gcaattatat ctctcgccag cagaatgatt ttcacgttta
    20701 ctatgaacca cctttttatt tccgccgtga gggattatgc gtttccgcca gttgttaccg
    20761 ctttttggcg cgctgtttgc gttgtatatc atttggggct caacctattt tgtcattcgg
    20821 attggcgtgg gaagctggcc tccgttaatg atggcgggcg ttcgattcca ggcagccggt
    20881 attttattgc tggcattttt gctactgcgc ggacacaaac ttcccccgct acgtccgctg
    20941 ctcaatgccg cgctgattgg cctgttattg ctggctgtcg gtaatggcat ggtgacggtt
    21001 gccgaacatc aaaatgttcc ttccggcatc gccgccgtag tgattgcaac cgtgcccctc
    21061 tttaccctgt gcttcagtcg cctgtttggc attaaaacgc gcaaactgga atgggtgggt
    21121 attgccattg ggcttgccgg aatcatcatg ctcaatagcg gtggaaattt aagcggcaat
    21181 ccgtggggcg cgattctgat tttaatcggc tcgattagct gggcgtttgg ctcagtttat
    21241 ggctcgcgca ttaccttacc tgtagggatg atggcgggtg cgattgagat gctggcggca
    21301 ggcgtggtgt taatgatcgc atcgatgatt gcgggtgaaa aactgacggc gctcccttcc
    21361 ctgtcaggct tccttgcggt cggctatctg gcgctgtttg gttcgattat cgccattaac
    21421 gcttatatgt atttaatccg taatgtcagt ccggctctcg ccaccagcta cgcctacgtt
    21481 aacccggtgg tcgcggtctt gctgggtacg ggactgggtg gcgaaacgct gtcgaagatt
    21541 gaatggctgg cgctcggcgt aattgttttc gcggtggtac tggtcacgtt gggaaaatat
    21601 ctcttcccgg ccaaacccgt tgtcgcgcca gttattcagg atgcatcaag cgagtaaatg
    21661 aatcccctgc gtgtcgatct gcgcgctggc accttcgccg cagatccact cttccagacg
    21721 ctcggtaagc gccgcatccg tcagcttctc gcgcctacgt aacgcacact cccagacaat
    21781 cagtacgcgc cagccgagct cctgcaagcg actgatatcg cggcgatcgc gttcaacatt
    21841 tttacctatc ttctccagcc agaactcagt tcgagtcgca ggcactttaa acagatagca
    21901 gtgatgatga tgccagaagc agccgtgggt aaatatcacg cagcgatatt catcaacgac
    21961 aaaatccggg cgtccgggca gactggcgtc ctgaacgcga aacgcaaggc cctgcccggt
    22021 taacaaactg gcgaggcgct tctcaatcgc cgtatcacgc gtggcaatcg cgcgcatatt
    22081 tttgctgcgg gtggccttat cgtgaacgtc gaccatgttg tgcctcttgc tgacgcaacg
    22141 ccaccgcctg tttgattttt ggctcaagca gttttgccac cgcggcaaag accggcacga
    22201 ccaccgagtt gccgaactgg cgataggcct gagtgtccga aaccggaatg cggaatttcg
    22261 cttctcccgg cgcttcaaaa cccattaagc gcgcgcattc acgaggcgtt aaccgacgtg
    22321 gacgatgttg ctgattcagc ggatcgtcaa agtctttctc acccgtggcc atatcccaac
    22381 cgcgatcgat taaaatttcc gcgccatctt tgtaataacg agcagacagc gtacgggtga
    22441 cgctttgcgg attgttcgga taaaccattc cataaccgaa gccgttaccg cgcgcctgat
    22501 gttttttcgc atatcgatag aggtacttcc acagcaccgg cgtcaggata tatttcgcct
    22561 caaccatcgg gtccagcagc tgcgccagcg tcactcgctg tgcagggaaa cattcgctga
    22621 tatcacgcag agtaaaatcg gctttcagat tcagatcgcg acgaaaaccc accagcacga
    22681 tgcgttcacg gtgctgcggc aggaaatgtt taccatcgat gattttcgga tcgtccggcc
    22741 cgttatcttc tgcatcggca acgtcatagc ccagttcgtc cagcgtctgc atgatgatgc
    22801 ggaacgtttt tcccttatcg tgactcttca ggtttttgac gttttcgagc acaaacatcg
    22861 ccggacgacg cgcgtcgata atgcgcacca catcaaagaa cagcgtgccc tgggtatcgc
    22921 aggcaaaacc gtgcgcccgc ccgagcgagt tctttttcga tacgccagcc agcgaaaatg
    22981 gctgacaagg gaaaccggcc agtaaaacat cgtgttcagg aatgtgttga cgaatatgtt
    23041 ccgccgccgc ctcatcactc acgccttctt gatgactgag ggtgatgtcg cggatatctt
    23101 cattaaaatg atgcgtcgcc gggtcgcaat aatggttggc tttataagtg cgtaccgcat
    23161 gtttgttcca ttcgctggta aacacgcact gtccaccaat cgattcaaaa ccacgacgga
    23221 tgccgccaat tccggcaaat agatcgataa agcgaaacgc ataatgcgga tgatgttccg
    23281 gtggtttggg taacaacgtt tgcagatggg caaactcttt ctcacttaaa cggtgccatg
    23341 ccgagtcatt cgccagcgca cgttttaaaa ttgccgcgct ccagtgattc tcacccacac
    23401 cattaagctg cgccaccagc attttaacat cataaatttg cagcagtttc tccagcattg
    23461 cctgtgcggc attcccggtg ctgtatgaat cggttactga tatattttcc tgcatagatt
    23521 taaccggcca tctgagatgg ccgacaggtt aacataatta ctgccgttta gggccacgcg
    23581 ctcagccagc gggtgaaaac gcatctatca ccttgtgatc gataccttca ttctcccccg
    23641 ccagttcggc accgagcttc gccatcaaac agcgccaagc tcaatgcccc tgaaacggca
    23701 aatacgcgcg ccaggccaat cgcccccagg gcttccagcc gatccgcatc ctggacgatt
    23761 ttagcttccg ttgttaaggg ggcgatttgc gcgctgaaac tgtgagcggc aatggcatga
    23821 caaacggcct caattttctc cgccggaaat tgcacgaact cttcacggag caggcgacgc
    23881 gtctcttctg ccgccaggat tgaagaacgc tgccgttgcg gatgattttt cgccaggctc
    23941 acgatatcgt gaaaataaca ggcggttaaa atcaccagca tatcgacatc gtcatctgcc
    24001 gccagtttct gtgccgtagc ccagacacgg cgaaaatgac acaggtcatg cgccgcgtcc
    24061 tgatgttgat gatggttttt caaccagttc tcgaactatg cctgccagtg ttgtaactcc
    24121 atacgcgctc cgttgccaaa gagtctcaac cttagcagtt tttattcata gcgtctgcaa
    24181 cgtgcgggac gaggtcgggt gtaccatgca atgccgccta acaaggcacc aaccagggcc
    24241 ataaacagaa atccgaccaa tgctcctaac cacttccccg cggtagatcc taaatcactt
    24301 tcaaatcccg acacgggggc atcggggatc cggctcatca cccagatgta tcgcgccatt
    24361 gcccagacga aatagccaca ccatgtatag aaagccc
//
LOCUS       DAZSNM010000043        24223 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZSNM010000043 DAZSNM010000000
VERSION     DAZSNM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24223
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.043"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(9..890)
                     /gene="prmA"
                     /locus_tag="ACHW42_001447"
     CDS             complement(9..890)
                     /gene="prmA"
                     /locus_tag="ACHW42_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR8943325.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(902..2353)
                     /gene="panF"
                     /locus_tag="ACHW42_001448"
     CDS             complement(902..2353)
                     /gene="panF"
                     /locus_tag="ACHW42_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR8943326.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2343..2585)
                     /locus_tag="ACHW42_001449"
     CDS             complement(2343..2585)
                     /locus_tag="ACHW42_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR8943327.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2694..4043)
                     /gene="accC"
                     /locus_tag="ACHW42_001450"
     CDS             complement(2694..4043)
                     /gene="accC"
                     /locus_tag="ACHW42_001450"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR8943328.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4054..4524)
                     /gene="accB"
                     /locus_tag="ACHW42_001451"
     CDS             complement(4054..4524)
                     /gene="accB"
                     /locus_tag="ACHW42_001451"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR8943329.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5502..6476)
                     /locus_tag="ACHW42_001452"
     CDS             complement(5502..6476)
                     /locus_tag="ACHW42_001452"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR8943330.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6628..8568
                     /gene="csrD"
                     /locus_tag="ACHW42_001453"
     CDS             6628..8568
                     /gene="csrD"
                     /locus_tag="ACHW42_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR8943331.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            8873..9916
                     /gene="mreB"
                     /locus_tag="ACHW42_001454"
     CDS             8873..9916
                     /gene="mreB"
                     /locus_tag="ACHW42_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR8943332.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            9982..11085
                     /gene="mreC"
                     /locus_tag="ACHW42_001455"
     CDS             9982..11085
                     /gene="mreC"
                     /locus_tag="ACHW42_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR8943333.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11085..11573
                     /gene="mreD"
                     /locus_tag="ACHW42_001456"
     CDS             11085..11573
                     /gene="mreD"
                     /locus_tag="ACHW42_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR8943334.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11582..12175
                     /gene="yhdE"
                     /locus_tag="ACHW42_001457"
     CDS             11582..12175
                     /gene="yhdE"
                     /locus_tag="ACHW42_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR8943335.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12165..13634
                     /gene="rng"
                     /locus_tag="ACHW42_001458"
     CDS             12165..13634
                     /gene="rng"
                     /locus_tag="ACHW42_001458"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR8943336.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13702..17502
                     /gene="yhdP"
                     /locus_tag="ACHW42_001459"
     CDS             13702..17502
                     /gene="yhdP"
                     /locus_tag="ACHW42_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR8943337.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17658..19103
                     /gene="tldD"
                     /locus_tag="ACHW42_001460"
     CDS             17658..19103
                     /gene="tldD"
                     /locus_tag="ACHW42_001460"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR8943338.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19231..20160)
                     /gene="aaeR"
                     /locus_tag="ACHW42_001461"
     CDS             complement(19231..20160)
                     /gene="aaeR"
                     /locus_tag="ACHW42_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR8943339.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20343..20546
                     /gene="aaeX"
                     /locus_tag="ACHW42_001462"
     CDS             20343..20546
                     /gene="aaeX"
                     /locus_tag="ACHW42_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR8943340.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20554..21486
                     /gene="aaeA"
                     /locus_tag="ACHW42_001463"
     CDS             20554..21486
                     /gene="aaeA"
                     /locus_tag="ACHW42_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR8943341.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21492..23459
                     /gene="aaeB"
                     /locus_tag="ACHW42_001464"
     CDS             21492..23459
                     /gene="aaeB"
                     /locus_tag="ACHW42_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR8943342.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23551..23823
                     /locus_tag="ACHW42_001465"
     CDS             23551..23823
                     /locus_tag="ACHW42_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR8943343.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(23879..24142)
                     /gene="yhcN"
                     /locus_tag="ACHW42_001466"
     CDS             complement(23879..24142)
                     /gene="yhcN"
                     /locus_tag="ACHW42_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR8943344.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5742 a         6103 c         6578 g         5800 t
ORIGIN      
        1 tgcgaaggtc aattcttacg accggtaata cggcaccact cttctttttc cacgaccggg
       61 tccagtgcga agctatcggc ataagcttca caaacgctct ctgcctggct tgccagaata
      121 ccggaaaggc ccagcaaacc gcctgaaact ggcaggacgc tgattaacgg tgccagttca
      181 cgtaatgggc ctgcaaggat gttagcgacc accacgtcgg ctttcatttc ttctggctga
      241 tctttcggta agtagagttc cagacggtca gaaacgccat tacgttcggc gttatcgcgg
      301 ctggcctgaa tcgcctgcgg atcgatatca ataccaatgg cttttgctgc acccagtttc
      361 agcgccgcga tcgccagaat gccggaacca cagccaaagt cgatgactgt tttaccggtt
      421 aaatcgaggc tgtcgagcca ttgcaggcac agagaggtgg ttggatgggt acccgtacca
      481 aacgccagcc ctggatctaa catcacgttg acggcgtttt cgtccggcac atcacgccag
      541 ctagggcaga tccacagtcg ttcaccaaag cgcatcgggt ggaaattatc catccattcg
      601 cgctcccagt ctttatcttc tagttgttcg attttatgcg cgaagcctgc gccgagcagc
      661 ggatggtttt ccagaatcgc caccacgtcg ttcatatcgg tttcagcgtc gaacagacca
      721 atcacatcgg tgtcgcccca caggcgcgtt tcgcccggca gcggctcaaa tactggcgta
      781 tcgtgggtat cctgaaaagt gatagaaacg gcacccgctt ccatcagcgc atcgctaaga
      841 tcttccgcgt tcgcgccggt ggtgttcagt ttcagttgga tccaaggcat ggcaaaactc
      901 tttatttatc agtagtcaaa acggtagctt gcgggacgga tgtaccgaaa cggtttccga
      961 ccaggaaagc cagcaaactt agtagtaacg agggcacgat agggtggaag cccaggtact
     1021 gaatattcag cgtcgcgagt acggcataca gcacgccgcc aacgatcatc gcacttagcg
     1081 cgcctttggc gttggcgcgt tcccagtaaa gacccagcac cagcggccac aggaaaacgg
     1141 cttccagccc accgaaggcc agcaaattca gccagatgat catttctggc ggcttccagg
     1201 cggcaagcag cagcaacgcg ccgagaacta acgtaattac cgccgacatc cgcttcagac
     1261 gcgtctcgtt ttgcatttga tccggacgga tattcagata gagatcttta atgatcgtag
     1321 cggaactttg cagcagttgg gcgttaattg tcgacatgat cgcagccata ggtgcagcca
     1381 gaaagatccc ggcagcaaaa ggtggcagca cttttaccat taacgtcggg atcaccagat
     1441 ctggtacggt gagatcgggg atcactgcac gacctaacgc tccggccagg tgcataccga
     1501 acatcaggat tgcgactata atcgtaccga tgatgatccc ccggtgtact gctttgctgt
     1561 ctttataaga gatacagcgc accgcagtat gcggcaggcc aatcacgcca aagcacacca
     1621 gtacccagaa cgacgtcata aaggcaggcg acagaatatc gtcagcgcct tgtggcgtaa
     1681 ccagttgcgg atcgatggtt tgcaaggtct gtactgcgtt gcttaagccg ccagcggcat
     1741 gtaccacgcc aataagcagc acaacggtgc caatcagcat cacaagccct tgcatggtgt
     1801 cgttcagcac gctggcgcga aagccaccaa aggcggtata taacgcaatg ctgataccaa
     1861 aaatcagcag cccggtttca taaggaatac ccgccgcggt ttccagcagg cgcgcaccgc
     1921 cgataaactg cacggtcatc gcaccaacga acgcaaccag caaactcaaa ctcgccagcc
     1981 acaccagaag acgactctgg tagcgggcaa acagcatatc gttcagcgtc actgcattgt
     2041 agcggcgcgc aagaatcgca aacttcttgc cgagaatacc gagtgaaagc cagacagcag
     2101 gaagctgaat catcgccagc aatacccagc ccagcccgta tttataagca gctcctggcc
     2161 cgccgataaa cgaactggca ctgatatagg tcgcggtgag agtcatcgcc agcacaatac
     2221 cgcccataga gcggctgccg aggaaatact cattaaggaa ggtgccggtg ctccgtttac
     2281 gcatcgcata aaccgagata ccgaacacca ccaccagata ggcgaccagc ggtagaatta
     2341 cttcaagctg catcgtcatc ctccagtggg atatcgcgat agataaattt caccatcgcc
     2401 cagcacagtc caataaacag tagcggcgtc aggatgcagg ccatctcaaa ccagcgcgga
     2461 aagccggtaa aaccgggggc aacgccaggt aagtaagcgt ctactaacca aactgccaga
     2521 tacaaaaggg tcagccccag cgcccagcgg gcctctttat gggcctgaac aaaacgagtg
     2581 tccatttttt gtccctgatg ggtgaagaaa gcgaggattg taccttatgg gggttgtcga
     2641 tccccagtaa taaaaaaggc cggaaaatcc ggccttttga cgcttttgca gtcttatttt
     2701 tcctgaagac cgagtttttt ctccagatag tggatgttag tgccaccatg ctggaagttc
     2761 tcgtcattca tgatgcggat ctgcagatca acgttggttt tgataccgtc gatgatcagc
     2821 tcctgcagcg cattcttcat gcgggcaatc gccacgtcac ggttttcacc gtagcaaatc
     2881 agcttaccga tcattgagtc atagtacggc ggtacggtgt agcccgcgta gatatgagac
     2941 tcccaacgta cgccaaaacc gccaggtgcg tggaaacggg tgattttgcc tgggcttggc
     3001 aggaaggtgt tcggatcttc ggcgttgata cgacattcca ccgcatggcc gcgaacgtga
     3061 acttcttctt gcttgatcga cagcggttga ccggcagcga tacgcagctg ttctttgatc
     3121 aggtcaacgc cggtgatcat ttctgtaacc gggtgttcta cctgaatacg ggtgttcatt
     3181 tcgatgaaat agaactcgcc gttttcgaac aggaactcga aagtacctgc accgcgatag
     3241 ccgatatcaa cacacgcttt agcgcaacgt tcgccgatgt agcgacgcag ttccggggta
     3301 atgcccggtg ctggtgcttc ttcgaccact ttctggtggc ggcgctgcat ggagcagtca
     3361 cgttccgcca gatagatagc gttgccctga ccgtcagcca gtacctgaat ctcgacgtgg
     3421 cgaggattct ccaggtattt ctccatgtaa accatatcgt tgctgaaagc agctttcgct
     3481 tccgcacggg tcatggagat ggattgtgcc agttcagcgt cgccgcgcac tacgcgcata
     3541 ccgcgaccgc cgccgccgcc ggaggctttg ataatcaccg gataaccaat gcgtttagca
     3601 atggcgcggt ttttatccat atcgtcgccc agcgggccgt cagaaccagg tacgcaagga
     3661 acgcccgctt ttttcatcgc cgcgattgcg gatactttgt cgcccatcag gcgaatggtt
     3721 tctgctttcg ggccgatgaa gataaagccg gagcgttcaa cctgctcggc aaagttggcg
     3781 ttctcggaga ggaagccgta acccggatgg attgctactg cgccggtgat ttcagcggcg
     3841 ctgatgattg ccgggatgtt cagataactt tttactgacg gagcagggcc aatacagacc
     3901 gtttcatctg ccagtaatac gtgttttaga tcgcgatccg tgctggagtg cacagcgaca
     3961 gtcttgatgc ccagttcttt acaggcacga agaatacgca atgcaatctc gccgcggttg
     4021 gcaataacaa ttttatccag catgttcgcc tcgttactcg atgacgacca gcggctcgtc
     4081 aaattctacc ggttgtccac tttcgaccag aattgctttc acggtaccgg atttgtccgc
     4141 ttcgatctgg ttcatcattt tcatggcttc aacgatgcac agggtatcgc ccacgttgac
     4201 tttctgaccc acttcgatga acgcttttgc gtccgggctt ggggtgcggt agaaagtacc
     4261 aaccatcggg gaacgtacga tgtgaccact gatttccgct gctgctggcg cttccatgga
     4321 aggaacggtc gccggagcgg ctgcgttaga ttgagctggc tgctgcatca ttggtgcagc
     4381 gtaagcttgt tgcatcacag ggaaacttgc ggcaggagct gcacggctaa tgcgtactga
     4441 ctcttcgcct tcagaaattt ccagttcgga gatgcctgat tcttcaacca gctcgatcag
     4501 ttttttaatc ttacgaatat ccatgagtgg gttccgtact ctttgtttag tgtgattgtg
     4561 acaggcgttt caccgccgtc tgtaaagcgt atgccccgac agctgtatgc atagcgataa
     4621 attccagcag gccgggacgc cggtctattt tgccccagag tgtcaatgtt agacttgacg
     4681 gacattgtgc agcgatctgc tgctatcctc cggcaaaaaa caaaatatac cttcgttgcg
     4741 ccaatgtcac cttttccgca gcgcaaaaac tgcgtcaggg aggacgaggt cgcacattat
     4801 aacgatttcg tagcaattgg cagctaaata ctggtcttat cagggaatat aatcaacagc
     4861 taacatgtaa ataaccttca acaccgtgta atttgcaaca agccgcacaa ttcacgaaat
     4921 tagcgccatc atcgacggaa cttcttataa cgtaaggtga aaagctgaaa agccagccct
     4981 gcgaagatga ggggctgcaa tgagataatc ttcacagacc acaaataatg tagggatgcc
     5041 aggatcgcga caagatagac gacattacgc agcggttgct ggggattacc caattttcac
     5101 tgcatcgtct ggatagatgt gaatgctaaa gcacgcaaaa tcaaccaact gacaattctc
     5161 agcattaaat aggttcgagt ggttaatttg tagtcaaata aaatcagatt attcacccca
     5221 tggtaccaga aatcagtaac aggttaagcg tagtgtcgcc caggcaaagc accataaacc
     5281 taacaggcga cggaggcgta tcaataatgg ctgtttagag gagtatcaat gcgcatcaat
     5341 ttgtggtcag tcgaaatcat gactttccag tttggatgat gttgtacaac ttcctgacca
     5401 aggtgttcga cgaaagctgt ccggctaacc gcttttcccg ttaaccgatt gaataatgat
     5461 gtcaaaatga gttccctctc ttattattcc ttgctaaatg gttagttaac cttcaccagc
     5521 gtgcgaccct ggatctggtt attaatgatg gcctcggcaa agttcggtgc ctctgacaga
     5581 gatatctctt ttgccgcctg ggtatagaat gattccggta aatcggcgac cagtcgctgc
     5641 caggcttgtg cgcggcgttc tggtggcgtc attactgaat ccaccccttg caaacggaca
     5701 ttacgcagaa taaatggcat gaccgtggtt ggcagagtaa aaccacccgc cagaccacag
     5761 gccgccacgc agccgccgta attcatttgc gccagcactt ttgccagcac tttgtcgcca
     5821 acagtgtcaa ttgccccagc ccagacttgt ttttccagag gacgggattc ggcaaactca
     5881 tcacgaggga gaataccgct ggcacctaaa cttttcagat attcatgggt actttcgcga
     5941 ccggaaacgg caacgacctg ataacccaac ttatgcagca gcgccacggc ggtactgccg
     6001 acgccaccac tggcacccgt cacgacaatc tcaccgtcct gcgggcgaac accggcatct
     6061 tccagcgcca tcacacacag catggcggta aaaccggcag taccgataat cattgcttta
     6121 cgcgcgtcca gcccttgcgg catggcaacc agccagtcac ctttcactcg cgcctgctcc
     6181 gccagcccac cccagtggtt ttcaccaacg ccccagccag tgagtaacac ctcctgaccg
     6241 gcatgaaaac gcggatcttc gctggtgcgt acagttccgg caaaatcgat cccaggaatc
     6301 atcggaaaat tacggatgat ttttccctta ccggtaatcg ccagcgcatc tttatagttc
     6361 aggctcgacc agtgaacatc gaccgtgaca tcgccctccg gcaggcgacg ttcgtccaga
     6421 gtctgtactg atgcgagagt tttgccgtcc tgctgttcta aaagtaacgc ctgcataagt
     6481 ggtcctcatg tgcatgatgg attggaaaat aatttctgaa gactatactc gctaatggaa
     6541 aagcaaagcc gatgaagcgc aataaattgc gagataaatc tgatttgcta gtatgcccgc
     6601 ttcctcacta tcggagttaa cacaaggatg agattaacga cgaaattttc ggcctttgtt
     6661 acgctgctca ccgggttaac aatttttgtg actttgctgg gctgttcgct aagtttctac
     6721 aacgccattc agtataagtt tagtcatcgc gttcaggcgg tggcgacggc gattgatacc
     6781 caccttgtgt cgaatgactt cagcgcatta aggccacaaa ttaccgaatt aatggtgtca
     6841 gcagatatcg ttcgtgtaga cctgctgcat ggtgataaac aggtttatac cctggccaga
     6901 aatggtagtt atcgtccggt tggcaccagc gatctgtttc gtgaactgag cgttccgttg
     6961 ataaagcatc cggggatgtc gttgcgtctg gtttatcagg atccgatggg caactatttc
     7021 cattcgttga tgaccaccgc gccgctcacg ggggcgattg gctttatcat tcttatgctc
     7081 tttctggcgg tacgctggtt acaacggcaa cttgccgggc aagaattgct ggaaacccgg
     7141 gctactcgta tcttaaacgg tgagcgtggc tctaatgtgt tgggaaccat ctatgaatgg
     7201 ccgcccagaa ccagcagtgc gctggatacg ctgcttcgtg aaattcagaa cgcacgcgaa
     7261 caacacagcc gtcttgatac gctgatccgc tcttatgccg cacaggacgt gaaaaccggc
     7321 ctcaataacc gactcttttt cgataatcag ttagcaacgt tactggaaga tcaggagaaa
     7381 gtaggtaccc acgggatcgt gatgatgatt cgtctgccgg atttcaatat gttgagcgat
     7441 acctgggggc acagccaggt tgaagaacag ttcttcactc tgacgaatct gctgtcgaca
     7501 tttatgatgc gctaccctgg cgcactgctg gcgcgttacc accgcagtga ttttgctgcg
     7561 ctgttaccgc accggacgtt aaaagaggca gagagcatag ccagtcagtt aatcaaagcc
     7621 gtcgatacct tgccgaacaa taaaatgctc gatcgcgacg atatgatcca cattggtatc
     7681 tgtgcctggc gtagtggtca ggataccgag caggtaatgg aacatgcaga gtctgccacg
     7741 cgtaatgcgg gattgcaggg tggcaatagc tgggctattt atgatgactc gttgcctgaa
     7801 aaaggacgcg gtaatgttcg ctggcgtacg cttatcgagc aaatgctcag tcgcggcggc
     7861 ccgcgccttt atcaaaaacc ggcggttact cgcgaaggtc aggttcatca tcgcgaactc
     7921 atgtgccgca tcttcgatgg taatgaagag gttagctcgg cggagtatat gccgatggtc
     7981 ttgcagtttg gcttatcgga agagtatgac cgtctgcaaa tcagccgtct tattccacta
     8041 ttgcgttact ggccagagga aaatctggcg attcaggtta ccgttgagtc gctgattcgt
     8101 ccgcgttttc agcgttggct gcgcgatacg ttaatgcaat gtgaaaaatc acaacgaaaa
     8161 cgcataatta ttgaacttgc agaggccgat gtaggtcaac atatcagtcg tttacaacct
     8221 gttattcgtt tagtgaatgc tttaggggta cgggtagccg tcaaccaggc tggtttgacg
     8281 ctggtaagca ccagttggat caaagaactt aatgttgagt tactcaagct ccatccgggg
     8341 ctggtcagga acattgagaa gcgaacggag aaccagctgc tggttcaaag cctggtggaa
     8401 gcctgctccg ggaccagcac ccacgtttac gccaccggcg tgcgttcgcg aagcgagtgg
     8461 cagaccctga ttcagcgcgg tgttacaggc gggcaagggg attttttcgc gtcctcacag
     8521 ccacttgata ctaacgtgaa aaaatattca caaagatact cggtttaacc tgccgtttaa
     8581 tccgttttca cgtagaataa cgcgcgctgc gtctcatggg agtgtgcttg tctgctcgcc
     8641 agattgttgc agcacatatg cagatgaatg accttacgcg gttgcaaaca ggcgaggaat
     8701 gctgctgatg cattaagcct ttctggactc aggcagagat ttgtaacaaa ggaaacgaac
     8761 tgcactaatt ttcaccgtag cagatgattt ttgcgccttg tcgctgctgc gtgtggttgg
     8821 taaagtaagc ggattttctt ttccgcccca gctttcagga ttatccctta gtatgttgaa
     8881 aaaatttcgt ggcatgtttt ccaatgactt gtccattgac ctgggtactg cgaataccct
     8941 catttatgta aaaggacaag gcatcgtatt gaatgagcct tccgtggtgg ccattcgtca
     9001 ggatcgtgcc ggttcaccga aaagcgtagc tgcagtaggt catgacgcga agcagatgct
     9061 gggccgtacg ccgggcaata ttgctgccat tcgcccaatg aaagacggcg ttatcgccga
     9121 cttcttcgtg actgaaaaaa tgctccagca cttcatcaaa caagtgcaca gcaacagctt
     9181 tatgcgtcca agcccgcgcg ttctggtttg tgtaccggtt ggcgcgaccc aggttgaacg
     9241 ccgcgcaatt cgtgaatccg cgcagggcgc tggtgcccgt gaagtcttcc tgattgaaga
     9301 accgatggct gccgcaattg gtgctggcct gccggtttct gaagcgaccg gttctatggt
     9361 ggttgatatc ggtggtggta ccactgaagt tgctgttatc tccttgaacg gcgtggttta
     9421 ctcctcttcc gtgcgcattg gtggtgatcg tttcgacgaa gctatcatca actatgtgcg
     9481 tcgtaattac ggttctctga tcggtgaagc caccgcagaa cgtatcaagc acgaaatcgg
     9541 ttcggcttac ccgggcgatg aagtccgtga aatcgaagtt cgtggccgta acctggcaga
     9601 aggtgttcca cgcggtttta ccctgaactc caacgaaatc ctcgaagcac tgcaggaacc
     9661 gctgaccggt attgtgagcg cggtaatggt tgcactggaa cagtgcccgc cggaactggc
     9721 ttccgacatc tccgagcgcg gcatggtgct caccggtggt ggcgcactgc tgcgtaacct
     9781 tgaccgtttg ttaatggaag aaaccggcat tccagtcgtt gttgctgaag acccgctgac
     9841 ctgtgtggcg cgcggtggcg gcaaagcgct ggaaatgatc gacatgcacg gcggcgacct
     9901 gttcagcgaa gagtaatcgg atgcaggcag gggaagcgtc tgtttaccct gcctggtctg
     9961 atacgagaat acgcataact tatgaagcca atttttagcc gtggcccgtc gctacagatt
    10021 cgccttattc tggcggtgct ggtggcgctc ggcattatta ttgccgacag ccgcctgggg
    10081 acgttcagtc aaatccgtac ttatatggat accgccgtca gtcctttcta ctttgtttcc
    10141 aatgctcctc gtgaattgct ggatggcata tcgcagacgc tggcctcgcg tgaccaacta
    10201 gaacttgaaa accgggcgtt acgtcaggaa ctgttgctga aaaacagtga actgctgatg
    10261 cttggacaat acaaacagga gaacgcgcgt ctgcgcgagc tgctgggttc cccgctgcgt
    10321 caggatgagc agaaaatggt gactcaggtt atctccacgg ttaacgatcc ttatagcgat
    10381 caagttgtta tcgataaagg tagcgttaat ggcgtttatg aaggccagcc ggtcatcagc
    10441 gacaaaggtg ttgttggtca ggtggtggcc gtcgctaaac tgaccagtcg cgtgctgctg
    10501 atttgtgatg cgacccacgc gctgccaatc caggtgctgc gcaacgatat ccgcgtaatt
    10561 gcagccggta acggttgtac ggatgatttg cagcttgagc atctgccagc gaatacggat
    10621 attcgtgttg gtgatgtgct ggtgacctcc ggtctgggcg gtcgtttccc ggaaggctat
    10681 ccggtcgcgg ttgtctcttc cgtaaaactc gatacccagc gcgcttatac tgtgatccag
    10741 gcgcgtccga ctgcaggact gcaacgtttg cgttatctgc tgctgctgtg gggcgcggat
    10801 cgtaacggcg ctaacccgat gacgccggaa gaggtgcatc gtgttgctaa tgaacgtctg
    10861 atgcagatga tgccgcaggt attgccttcg ccagacgcga tggggccaaa gttacctgaa
    10921 ccggcaacgg ggatcgctca gccgactccg cagcaaccgg cgacaggaaa tgcagctact
    10981 gcgcctgttg cgccgacaca gcccgctgct aatcgctctc cacaaagggc tacgccgccg
    11041 caaagtggtg ctcaaccgcc tgcgcgtgcg ccgggagggc aatagtggcg agctatcgta
    11101 gccagggacg ctgggtaatc tggctctctt tcctcattgc gctgttgctg caaatcatgc
    11161 cctggccgga taacctgatt gttttccggc caaactgggt gttacttatc ttgttgtatt
    11221 ggatcctggc cttgccccat cgcgtaaatg tgggcacagg ttttgtgatg ggtgccatac
    11281 tggatctgat cagcggctcg atgcttggcg tacgcgtatt ggcgatgagc atcattgctt
    11341 acctggtggc gctgaaatac cagcttttcc gcaacctcgc attatggcag caggcgctgg
    11401 tcgtcatgtt gctttcgctg gtggtggata ttattgtttt ctgggcagag tttttagtga
    11461 ttaacgtctc tttcagaccg gaagtgttct ggagtagtgt agtcaatggg gtgctctggc
    11521 cgtggatttt cttgctgatg cgtaaagtcc gtcagcagtt tgcagtgcaa taaaggtttc
    11581 tatgacttct ctgtatttag cttccggttc tccgcgtcgt caggagttac ttgcgcaact
    11641 tggcgtgacc tttgaacgta ttgttacggg cattgaggag cagcgtcagc cgcaggagag
    11701 cgcgcagcag tatgttgtgc gtctggcacg cgagaaagca caggcgggtg tcgcgcaaac
    11761 ggcacaggat ctcccggtgc tgggtgcgga tattatcgtt atcctgaacg gagaagtgct
    11821 cgagaaaccg cgcgacgcag agcatgcggc gcagatgttg cgcaaattat cgggtcagac
    11881 ccatcaggtg atgacggcag tggcgttggc cgacagccag cacattctcg attgcctggt
    11941 ggtcaccgat gtgactttca gaacgttaac agacgaagac atcgcgggct atgtcgccag
    12001 cggtgaaccg ttagataaag caggtgcata cggtattcag gggctgggtg gctgttttgt
    12061 caggaagata aatggcagct atcacgccgt agtcggctta ccgctggttg aaacgtatga
    12121 attattaagt aattttaacg cactgcgtga gaaaagggat aaacatgacg gctgaattgt
    12181 tagtaaacgt aacgccttcg gaaacgcgag tggcgtatat tgatggcggt attctgcagg
    12241 aaattcatat tgaacgtgag gcgcgacgcg gaatagtagg caatatctac aagggtcgtg
    12301 taagtcgtgt acttccgggt atgcaggcgg cttttgtaga tattgggctg gataaagccg
    12361 cgtttcttca tgcatccgac atcatgccgc ataccgaatg tgtggcgggt gaagaacaaa
    12421 agcaattcac ggtgcgcgac atctcggaac tggttcgtca ggggcaagat ctgatggtgc
    12481 aggtggtgaa agatccgctt ggcactaaag gtgcgcgcct gaccaccgat atcacgctgc
    12541 cttctcgcta tctggtattt atgccagggg cttctcacgt tggggtttcc caacgaattg
    12601 aaagcgaatc agaacgtgaa cgtctgaaaa aagtggtcgc agagtattgc gacgagcagg
    12661 gcgggtttat catccgcacc gcagcggaag gggttggcga ggctgaactg gcctccgatg
    12721 ccgcttatct gaaacgcgtc tggaccaaag ttatggagcg taaaaagcgc ccgcagaccc
    12781 gttatcagct gtacggcgaa ctggcgctgg cgcagcgtgt tctgcgtgat tttgccgacg
    12841 ccgaactgga ccgcattcgc gttgactcac gcctgactta cgaagcattg ctggagttta
    12901 cctcggagta cattcccgag atgaccagca agctggagca ttacaccgga cgccagccga
    12961 ttttcgatct ctttgatgtc gaaaacgaaa tccagcgagc gttggaacgc aaagtagaac
    13021 tgaaatccgg tggttatctg attatcgacc agaccgaagc gatgaccacc gtggacatca
    13081 ataccggagc gtttgtcggt catcgcaatc tggacgacac tattttcaat accaatattg
    13141 aagcgacgca ggctatcgct cgccagttac ggttgcgtaa tctgggcggg attatcatta
    13201 ttgatttcat cgatatgaat aatgaagatc accgccgccg cgtgctgcat tcgctggagc
    13261 aggcgttgag caaagaccgg gtgaaaacca gcgttaatgg tttttcggcg ctggggctgg
    13321 tggagatgac gcgtaaacgc acccgcgaaa gcattgagca cgtactgtgt aacgaatgcc
    13381 caacctgcca cggtcgcgga acggtgaaaa ccgtggaaac ggtatgctat gaaatcatgc
    13441 gcgagattgt tcgtgtccac catgcttacg actccgaccg tttcctggtc tatgcttctc
    13501 cggcagtagc cgaagccttg aaaggcgaag agtcacactc gctggcggaa gtggaaattt
    13561 tcgttggcaa acaggttaaa gtacaaattg aaccgctcta taaccaggag cagtttgacg
    13621 tcgtaatgat gtaaacagat gctgggccgc catccggcaa agggtttttg agtcacattt
    13681 ttagcagaca aggagtgacg ggtgaggcga ttgccgggga ttttactgct tactggagcc
    13741 gcgctcgttg tgatcgctgc cctgctggtt agcggcctgc gtattgcttt accgcatctt
    13801 aacgcctggc gtccggaaat cctcaacaaa atagaatccg cgactggcat gccggtagaa
    13861 gccagtcagc tctcagccag ctggcagaat tttggcccga cgcttgaagc acacgacatc
    13921 cgtgcagaac taaaagatgg cggcgaattt tcggttaaac gcgttactct ggcgctggat
    13981 gtctggcaga gcctgttaca tatgcgctgg cagtttcgcg acctcacttt ctggcagctg
    14041 cgctttcgca ccaacactcc tatcaccagc ggtggtagcg atgacagtct ggaagccagt
    14101 cacatcagcg atctgtttct tcgtcaattt gaccatttcg atctccgcga cagtgaagtc
    14161 agttttctga cgccatccgg tcagcgcgcc gagctggcga tcccacaact cacctggctg
    14221 aacgatccac gtcgacaccg tgcggaaggc ctggtaagcc tctccagcct taccggacag
    14281 cacggcgtga tgcaggtgcg catggatttg cgcgatgatg aggggttgtt aagcaatggt
    14341 cgcgtctggc tacaggcgga tgacatcgac ctgaagccgt ggctcggtaa atggatgcag
    14401 gacaatattg ctctggaaac ggcacagttc tcccttgaag gctggatgac gatcgacaaa
    14461 ggcgatgtaa ccggcggtga cgtctggctg aaacagggcg gtgccagctg gttgggcgag
    14521 aagcaaacgc atacgctgtc ggtggataat ctgaccgcgc atattacgcg tgaaaatccg
    14581 ggctggcagt tctctattcc cgatacacgg atcacgatgg acggcaaacc ctggccgagc
    14641 ggagcattga cgctggcctg gataccggaa caggacgttg gcggcaaaga caataaacgc
    14701 agtgacgaac tccggattcg cgctagtaat ctggagctgg caggcctgga gggcatacgc
    14761 ccgctggccg cgaaactttc acctgcactg ggtgatgttt ggcgctccac acaaccgagc
    14821 ggcaagatta acactctggc gctggatatc ccgcttcagg cggcagacaa gacccgtttt
    14881 caggcatcgt ggagcgatct ggcctggaag caatggaaat tattaccggg tgcggaacac
    14941 ttctccggga cgctttccgg cagcgttgaa aatggtttgc ttaccgcgtc gatgaagcag
    15001 gcaaagatgc cttacgaaac ggtattccgt gcgccactgg aaatcgccga cggccaggca
    15061 actataagct ggctgaacaa tgacaaaggt ttccagctgg atgggcgtaa tattgacgtt
    15121 aaagccaaag ccgtccatgc gcgcggcggt tttcgttacc tgcaacctgc taacgatgaa
    15181 ccctggctgg gtattctggc tggcatcagt accgatgatg gttcacaagc ctggcgctat
    15241 ttcccggaaa acttgatggg taaagacctg gttgattatt taagtggtgc gattcagggc
    15301 ggtgaagcgg ataacgcgac gctggtttat ggtggcaatc cgcaactctt cccctataaa
    15361 cacaacgaag gtcagtttga agtgctggtg ccgctgcgca acgcgaagtt tgccttccag
    15421 ccggactggc ctgcattaac taaccttgat attgaactgg actttattaa cgacggttta
    15481 tggatgaaaa ccgatggcgt taatctgggc ggcgtgcgcg cgagtaatct caccgcagtg
    15541 atccctgact actcaaaaga aaaactgctg attgacgctg acattaaagg tccgggtaaa
    15601 gccgttggcc cttactttga tgagacaccg ctgaaagatt ctctgggtgc gaccctgcaa
    15661 gaactccagc tcgacggcga tgtgaatgct cgcttacatc ttgatatccc gctgaacggc
    15721 gaactggtaa ccgcgaaagg tgaagtgacg ctgcgtaata acagtctgtt tatcaaacca
    15781 ctcgacagca tcctgaaaaa tttgagcggt aaattcagct ttatcaatgg cgatctgcaa
    15841 agtgaaccac tgacagcaag ctggtttaat cagccgttga acgtggattt ttccaccaaa
    15901 gaaggggcaa aagcctacca ggttgcggtg aatctcaacg gtaactggca accggcgaaa
    15961 accggcgttc tgcctgaagc ggtgaacgaa gcattgagtg gcagcgtggc gtgggatggt
    16021 aaagtgggca ttgatctgcc ttatcatgct ggcgcgacct ataacgtaga gctgaacggc
    16081 gatctgaata atgtgagtag tcacttacct tcaccgttag ccaaacctgc gggtgaacca
    16141 ctagcggtaa acgttaaggt tgatggcaat ctcaacagct ttgaattaac cggacaggct
    16201 ggtgcggata accatttcaa tagccgctgg ttgcttggtc aaaagctgac gctcgatcgt
    16261 gctatttggg cggcagacag taaaacgctc ccgccgttgc cggaacaaag tggcgttgaa
    16321 cttaatatgc cgccgatgaa tggtgccgag tggctggccc tgttccagaa aggcgctgcg
    16381 gagagtgtcg gtggtgcagc gagtttccca caacacataa cgttacgtac gcctatgttg
    16441 tcactgggaa atcagcaatg gaataacctg agtattgttt cgcaaccgac ggcaaatggc
    16501 accctggttg aggcgcaagg gcgtgaaatc aacgccacgc tagcgatgcg taataacgcg
    16561 ccgtggctgg cgaatatcaa atatctttat tacaacccga gcgtggcgaa aactcgtggt
    16621 gattcaacgc cgtcatcacc tttcccgaca acggagcgca ttaacttccg tggctggccg
    16681 gacgcacaaa tacgatgcac agagtgctgg ttctgggggc aaaaattcgg tcgcattgac
    16741 agtgatatca ccatttctgg cgatacgtta acgctgacca atggactgat tgatactggt
    16801 ttctcgcggc ttactgccga tggtgaatgg gttaataatc cggggaatga acgtacctcg
    16861 ctgaaaggaa aactgcgtgg gcagaaaatt gatgccgccg cagaattttt tggtgtcacg
    16921 acgcccatac gccagtcgtc atttaatgtg gattacgatt tacactggcg caaagcaccc
    16981 tggcagccag atgaagcgac gttgaatggc atcattcata ctcaactggg taaaggcgaa
    17041 attaccgaaa tcaataccgg acatgccggg caattgctgc gcttattgag cgtagatgcc
    17101 ctgatgcgta agctgcgttt tgatttcaga gacacttttg gcgaagggtt ctattttgac
    17161 tccattcgca gcaccgcgtg gattaaagac ggcgttatgc acaccgacga cacgctggtg
    17221 gatggcctgg aggcggatat cgccatgaaa gggtcggtaa atctggtacg ccgcgacctg
    17281 aatatggaag cggttgtcgc accagagatt tctgcgacgg tgggcgtggc tgcggctttt
    17341 gcggttaacc ccattgttgg cgcggcagtg tttgccgcca gtaaagtgct ggggccgctg
    17401 tggagcaaag tctccatttt gcgctatcac atttcgggtc cgctggacga tccgcaaatc
    17461 aacgaagtgt tgcgccaacc gcgtaaagaa aaagcgcaat gatttgacga gggcgcgtaa
    17521 ttgccccaat ctcatatgat aatcgttgcc aaaggccaac gagccggaac ataactgtag
    17581 gccggatagg gcgttcacgc cgcatccggc agccgtaaaa aatcctctac tgcagtaact
    17641 aacgagtagc aaaaacgatg agtctgaacc tggtaagtga acaattgcta gcggcgaacg
    17701 gcctgaaaca tcaggacttg ttcgcgatcc tcggtcaact ggccgaacgt cgccttgatt
    17761 atggcgatct ctattttcag tcgagctatc acgaatcctg ggttttagaa gaccgcatta
    17821 ttaaagatgg ttcttacaac atcgatcagg gcgttggcgt gcgtgcaatc agcggtgaaa
    17881 aaaccggatt tgcttacgct gaccaaatca gcctgctggc gctggaacag agtgcgcaag
    17941 cggcgcgcac catcgtccgt gatagtggtg atggcaaagt acagacgctg ggcgcggtag
    18001 agcatagccc gttgtatacc tcggtagatc cgctgcaaag catgagccgt gaagagaagc
    18061 tggatatcct gcgtcgcgtc gataaggttg ctcgcgaagc ggacaagcgc gtacaggaag
    18121 tgactgccag cctcagtggt gtctatgaat taattctggt tgcggccacc gacggcacgc
    18181 tagcggcgga tgtccgtccg ctggtgcgtc tttccgtgag cgttctggtc gaagaagatg
    18241 gcaaacgcga acgcggtgcc agtggcggcg gcggtcgttt tggttatgag ttcttccttg
    18301 ccgatcttga cggcgaagtc cgtgcggatg catgggcaaa agaagcagtg cgtatggcgc
    18361 tggtcaatct ttctgccgtt gctgcaccag cgggcaccat gccggtagta cttggcgcag
    18421 gttggccggg cgtgctgttg catgaagcgg taggtcacgg tctggaaggc gacttcaacc
    18481 gccgtggcac ttcagtattt agtggacagg tcggggagct ggtggcttca gaactgtgta
    18541 ccgtggttga tgacggcacg atggtcgatc gccgtggttc ggtggcgatt gatgatgaag
    18601 gtacgccagg ccagtacaac gtgctgattg agaacggcat tctgaaaggc tacatgcagg
    18661 ataaactcaa cgcgcgtttg atggggatga cgccgactgg caacggtcgc cgtgaatcct
    18721 acgcccatct gcccatgccg cgtatgacca acacctatat gctgccgggt aaatcgaccc
    18781 cgcaagaaat tattgaatcc gttgagtacg gtatctatgc accgaacttt ggtggcggtc
    18841 aggtggatat cacctccggc aaattcgttt tctccacttc agaagcatat ctgattgaaa
    18901 acggtaaagt aacgaagccg gtgaaaggcg caacgttgat tggttccggt atcgaaacca
    18961 tgcagcagat ttcgatggtt ggcaacgacc tgaaactgga taacggcgtg ggtgtctgcg
    19021 gtaaagaagg gcaaagtttg ccggttggcg tgggccagcc aacgttgaaa gtcgataacc
    19081 tgactgttgg cggtactgcg taataatttc attattttca gaaggataat taatctttct
    19141 acgtgcacga acggtcccct cgctcctctg gggttagggt gaggggaacc cgttggcaca
    19201 ggtttgtaga acgtaacagt acaatatgaa ttacttctct ttcccgcgcc cgtgcatctc
    19261 ctgaaacaat ttaccgacct caacaaaata atccgtcagc gagttgatca cgacctgtac
    19321 cttcagcggc agcttatctt tttcggtata taacgcataa accgggcgtg gatctgactg
    19381 ataacgcggc agcaggatct ccagctcccc acgattgatc tcgttgatca cccacatcag
    19441 cggcacgtag gcgatcccgg cacccgccgt tagccagcgc accagcgtca tcggatcatt
    19501 agtcacaaat ctcccttgcg ggatcaggcg agtcgagatc ccttccggtg caatcagttc
    19561 aaattcattg tcgggccgca cgctgtattc aagccatgaa tggctactta aatcggcggg
    19621 tttttccggt atgccgtatt gtgtgagata gcttttcgcg gcgcacacca ccattggcat
    19681 cgcgccaaga cggcgggaaa acaggctgga atcctgcaac gcgccgacgc ggatcaccac
    19741 atccagaccg tcggcaatca ggtcgggggc tggaattccg gtaaccagat tggcgctcaa
    19801 acctgggtat tctttcagca ttttggctgt cagcccggcg agaacatttt gtgccatagt
    19861 tgaagaacag ccaatgcgta gcgtcccgat gggggtgtta ttgaaggcat acagttgctc
    19921 atgaacatcc tgcacttcat gaagcatacg acggcagccc tggtagtaaa ttctaccggc
    19981 ttcggtcagg ccaatgctgc gtgtgctacg gtttaacagc tttacctgca actcatcttc
    20041 cagttttgac accgtctgac tgatggacga aacgctcatc tgtagctgtc tggcggcggc
    20101 ggtaaaagag ccaaattcaa ctactttggc aaacaccgac atgcgtttta gtcgttccat
    20161 tattcactct gacttaaaag tgatttagat cacataatat agataacagc ataacagtta
    20221 cgctaatata ttaaatatca atctacagca atgttgctct cgcccggctt tccatgccct
    20281 tctctgcggc gacagatgct gaaaataata acgcctgctc tctctataca accaaggtca
    20341 acatgagtct gtttcccgtt atcgtggtgt ttgggctgtc cttcccaccg atattttttg
    20401 aattgctttt atcactggcg attttctggc tggtgcgccg ggtacttgtg ccaacaggta
    20461 tctacgactt tgtctggcat ccggcgttgt tcaacaccgc gctctattgc tgcttgtttt
    20521 atttgatatc gcgactgttc gtttgaggtt gaagtgaaaa cactaataag aaaattctcc
    20581 cgtacggcca tcacggtcgt attagtcatt ctggccttca tcgcaatttt taatgcctgg
    20641 gtctattaca ccgaatcccc ctggacgcgt gacgcgcgct ttagcgctga cgtcgttgcg
    20701 atcgcgccgg acgtttctgg actcattacc caggtgaatg ttcatgataa ccagctggtg
    20761 aaaaaaggac agatactgtt caccatcgac cagccgcgct atcaaaaggc gcttgaggaa
    20821 gcgcaagccg atgttgctta ttatcaggta ctggcacagg agaaacgcca ggaggccgga
    20881 cgtcgtaacc gtctcggtgt gcaggcgatg tctcgcgaag agatcgacca ggctaacaac
    20941 gtactacaaa cggttctgca tcagttagcg aaagcgcagg cgacccgcga tctggcaaaa
    21001 ctggatcttg aacgcacggt gatccgagcg ccagcagatg gctgggtgac caacctcaac
    21061 gtctataccg gtgagtttat tactcgagga tcaacggcgg ttgcgctggt gaaacagaac
    21121 tccttctatg tactggccta tatggaagaa actaagctgg aaggggtgcg tccggggtat
    21181 cgtgcagaga tcacgccgct tggcagtaac aaagtgctga aagggactgt tgatagtgtt
    21241 gccgctgggg tcaccaacgc cagcagcacg cgtgacgaca aagggatggc gaccatcgac
    21301 tctgaccttg aatgggtgcg tttggcgcaa cgtgttccgg ttcgtattcg tctcgacaac
    21361 cagcaagaga acatctggcc tgcgggcacc actgctacag tggtggtcac tggcaaacaa
    21421 gatcgcgacg aaagccagga ttcgttcttc cgtaaaatgg cccatcgcct gcgtgagttt
    21481 ggttaatcac gatgggtatt ttctccattg ctaaccaaca tattcgcttt gcggtaaaac
    21541 tggcgaccgc cattgtactg gcgctgtttg ttggctttca cttccagctg gaaacgccac
    21601 gctgggcggt actgacagcg gcgattgttg ccgccggtcc ggcctttgct gcgggaggtg
    21661 aaccgtattc tggcgctatt cgctatcgtg gctttttgcg catcatcggc acatttattg
    21721 gctgtattgc cggactggtg atcatcattg cgatgatccg cgcaccatta ttgatgattc
    21781 tggtgtgctg tatctgggcc ggtttttgta cctggatatc ctcgctggta cgaatagaaa
    21841 actcgtacgc gtgggggctg gccggttata ccgcgctgat cattgtgatc accattcagc
    21901 cggaaccatt gcttacgccg cagtttgccg tcgaacgttg tagcgagatc gttatcggta
    21961 ttgtgtgtgc gattatggcg gatttgctct tttctccgcg atcgatcaaa caagaagtag
    22021 atcgagagct ggaaagtttg ctggtcgcgc aatatcaatt aatgcaactc tgtatcaagc
    22081 atggcgatgg tgaagttgtc gataaagcct ggggcgacct ggtgcgacgc accacggcac
    22141 tacaaggcat gcgcagcaac ctgaatatgg aatcttcccg ctgggcgcgg gccaatcgac
    22201 gtttaaaagc gatcaatacg ctatcgctga cgctgattac ccaatcctgc gaaacttatc
    22261 ttattcagaa tacgcgcccg gaattgatca ctgatacttt ccgcgaattt tttgacacgc
    22321 cggtagaaac cgcgcaggac gtccacaagc agctcaaacg cctgcggaga gttatcgcct
    22381 ggactgggga acgggaaacg cctgtcacca tttatagctg ggtcgcggcg gcaacgcgtt
    22441 atcagcttct caagcgcggc gttatcagta acacaaaaat caacgccacc gaagaagaga
    22501 tcctgcaagg cgaaccggaa gtaaaagtag agtcagccga acgtcatcat gcaatggtta
    22561 acttctggcg aaccacactt tcctgcattc tgggcacgct tttctggctg tggacgggct
    22621 ggacttccgg cagtggtgca atggtgatga ttgcggtcgt gacgtcactg gcaatgcgtt
    22681 tgccgaatcc acgcatggtg gcgatcgact ttatctatgg gacgctggcc gcgctgccgt
    22741 tagggctgct ctactttttg gtgattatcc ctaataccca acagagcatg ttgctgctgt
    22801 gtattagcct ggcagtgctg ggattcttcc tcggtataga agtacagaaa cggcgactgg
    22861 gctcgatggg ggcactggcc agcaccataa atattatcgt gctggataac ccgatgactt
    22921 tccatttcag tcagtttctc gacagcgcat taggacaaat cgtcggctgt gtgctcgcgt
    22981 tcaccgttat tttgctggtg cgggataaat cgcgcgacag gactggacgt gtactgctta
    23041 atcagtttgt ttctgccgct gtttccgcga tgactaccaa tgtggcacgt cgtaaagaga
    23101 accacctccc ggcactttat cagcagctgt ttttgctgat gaataagttc ccaggggatt
    23161 tgccgaaatt tcgcctggcg ctgacgatga ttatcgcgca ccagcgcctg cgtgatgcgc
    23221 cgatcccggt taacgaggat ttatcggcgt ttcaccgaca aatgcgccgc acagcagacc
    23281 atgtgatatc tgcccgtagc gatgataaac gtcgtcggta ctttggccag ttgctggaag
    23341 aactggaaat ctaccaggaa aagctacgca tctggcaagc gccaccgcag gtgacggaac
    23401 cggttcatcg gctggcgggg atgctccata agtatcaaca tgcgttgacc gatagttaag
    23461 tcaaaaccga cgccaaaagc gtcggttttt tcatggctat acttagcgat gcacggcaga
    23521 actcgccgcg aaacgtgacg gtggcaacag atgaatattt atgcctttga ttttgatgag
    23581 attgagagtc aggaggattt ttatcgtgac tttagccaaa cctttggtct ggctaaagat
    23641 aaggtacgcg atctcgactc actatgggat gtgttaatga acgatgtcct gccgctacca
    23701 cttgagattg aatttgttca tctgggagag aaaacgcgtc gccgttttgg cgcgttaata
    23761 ttgctgtttg atgaggcaga ggaagagctg gaagggcatt tgcgttttaa tgttcgtcat
    23821 tagcgcaaaa aaaagcccac gaaccggggg caatatcgtc ggacaaaacg atgagggttt
    23881 atttgtacag ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt
    23941 aggccgttgc gcctttctct tccgcttttt tgttcagcat ttcacgtata tccattggcg
    24001 aagacgccac accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag
    24061 catcaatgga gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag
    24121 ctacagtggt tttgattttc atgattttta cctcgacata atcttttagc tgggcctttg
    24181 tttcgtgacc catatcacaa aatcaagtat acactaatta ttg
//
LOCUS       DAZSNM010000044        23318 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZSNM010000044 DAZSNM010000000
VERSION     DAZSNM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23318
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.044"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHW42_001467"
     CDS             complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHW42_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR8943345.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGH"
     gene            348..641
                     /locus_tag="ACHW42_001468"
     CDS             348..641
                     /locus_tag="ACHW42_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR8943346.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="ACHW42_001469"
     CDS             713..1372
                     /locus_tag="ACHW42_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR8943347.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="ACHW42_001470"
     CDS             1464..1910
                     /locus_tag="ACHW42_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR8943348.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="ACHW42_001471"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="ACHW42_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /locus_tag="ACHW42_001472"
     CDS             3390..3809
                     /locus_tag="ACHW42_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR8943349.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5077
                     /gene="umuC"
                     /locus_tag="ACHW42_001473"
     CDS             3809..5077
                     /gene="umuC"
                     /locus_tag="ACHW42_001473"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR8943350.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="ACHW42_001474"
     CDS             complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="ACHW42_001474"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR8943351.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="ACHW42_001475"
     CDS             complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="ACHW42_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR8943352.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7561..8280
                     /gene="fadR"
                     /locus_tag="ACHW42_001476"
     CDS             7561..8280
                     /gene="fadR"
                     /locus_tag="ACHW42_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR8943353.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8332..9864)
                     /locus_tag="ACHW42_001477"
     CDS             complement(8332..9864)
                     /locus_tag="ACHW42_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR8943354.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10194..11492
                     /gene="dadA"
                     /locus_tag="ACHW42_001478"
     CDS             10194..11492
                     /gene="dadA"
                     /locus_tag="ACHW42_001478"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR8943355.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11502..12572
                     /gene="dadX"
                     /locus_tag="ACHW42_001479"
     CDS             11502..12572
                     /gene="dadX"
                     /locus_tag="ACHW42_001479"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR8943356.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12740..14154)
                     /locus_tag="ACHW42_001480"
                     /pseudo
     CDS             complement(12740..14154)
                     /locus_tag="ACHW42_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14765..16501)
                     /locus_tag="ACHW42_001481"
     CDS             complement(14765..16501)
                     /locus_tag="ACHW42_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR8943357.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="ACHW42_001482"
     CDS             complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="ACHW42_001482"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR8943358.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17610..18221
                     /gene="emtA"
                     /locus_tag="ACHW42_001483"
     CDS             17610..18221
                     /gene="emtA"
                     /locus_tag="ACHW42_001483"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR8943359.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18223..18957)
                     /locus_tag="ACHW42_001484"
     CDS             complement(18223..18957)
                     /locus_tag="ACHW42_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR8943360.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19158..19412
                     /locus_tag="ACHW42_001485"
     CDS             19158..19412
                     /locus_tag="ACHW42_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR8943361.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19462..21416)
                     /locus_tag="ACHW42_001486"
                     /pseudo
     CDS             complement(19462..21416)
                     /locus_tag="ACHW42_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(21442..22296)
                     /gene="modD"
                     /locus_tag="ACHW42_001487"
     CDS             complement(21442..22296)
                     /gene="modD"
                     /locus_tag="ACHW42_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFR8943362.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22296..23105)
                     /locus_tag="ACHW42_001488"
     CDS             complement(22296..23105)
                     /locus_tag="ACHW42_001488"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR8943363.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23115..>23318)
                     /locus_tag="ACHW42_001489"
     CDS             complement(23115..>23318)
                     /locus_tag="ACHW42_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8943364.1"
                     /translation="KNNGMTMLMSTHHTLHANAIADSIIQVEPDGRVTQGLPTEQLTT
                     NKLAALYRVSADQIHHHLSAISR"
BASE COUNT         5792 a         5697 c         5996 g         5833 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaatttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac cactgtatat
     3361 aaaaacagta taacttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagtgct attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
     3901 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gcgttaaaat
     3961 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
     4021 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
     4081 atcgccccgc gtcgagattt acagtattga tgaagcattc tgcgatctga caggtgtgcg
     4141 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     4201 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ttggctaagc ttgccaatca
     4261 tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg
     4321 ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat
     4381 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat
     4441 ccggtttatc cgtaaacatt ttaatgtcat gctcgaaaga acggtgcgtg aactgcgcgg
     4501 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     4561 ctcgtttggt gaacgcatca cggattatac gtcgatgcgg caggccattt gtagttacgc
     4621 tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat tgtcggttta tctccacatt
     4681 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa
     4741 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     4801 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     4861 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga
     4921 gcaattgatg gcggtaatgg atactctgaa tgctaaagag ggcagaggaa cactctattt
     4981 tgccgggcag gggatccaac aacaatggca gatgaagcga gctatgcttt caccacgtta
     5041 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgc
     5101 tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg
     5161 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     5221 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     5281 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     5341 cacaggtggc aaatggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     5401 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     5461 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     5521 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     5581 gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa cattggttca
     5641 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     5701 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa ttggacaatt atcgggcgaa
     5761 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     5821 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     5881 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     5941 atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta
     6001 gcgacagagg gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc
     6061 aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg
     6121 aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga
     6181 gcatgttcag atgcctgcaa cacaaactga ataatcggcg aaaacagttg ttggtcgata
     6241 atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg
     6301 aaggctttac cgatagcatg ttcatcggtg acaccggtca atgatgttgc cagaataatg
     6361 actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag
     6421 acgccaataa tcgcctggac aatcagacgg attttatcct gacgggtacg ctggtggcgg
     6481 ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag
     6541 ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga
     6601 acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg
     6661 atgatcaggt tctgtggttc gcccaccatg gtcattacgc cgcctaatgc ggtgccgaca
     6721 ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac cacgttgtaa
     6781 tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt cggtacggga agaggctacg
     6841 cgatgataaa taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag
     6901 gcatcgagga acgcggagag gaacgctgcc gccacgcaaa aagagagcga cagcagcatt
     6961 ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa
     7021 tagatacccg ccaccataaa catcaataac agcaagactt caagatttgc cgccacctct
     7081 tcacggacgt gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca
     7141 ccggggagta gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc
     7201 agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa
     7261 atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg
     7321 cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt
     7381 atgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg
     7441 gaaagagatg cggaaaatga agccttgatc cctttttctt ctttttgtct gctatcagcg
     7501 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     7561 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     7621 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     7681 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     7741 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta
     7801 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     7861 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     7921 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     7981 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     8041 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     8101 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     8161 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     8221 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     8281 tcccttccgt ttaaagagca accccctcaa acgaggggtt ttttgttgtt tttacagatt
     8341 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     8401 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc aggacttctt tgcgcccccg
     8461 atccagcggt gcgcgattat gtggaatata acgcagcgtc agcgaacggt cgccgcgcaa
     8521 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     8581 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     8641 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     8701 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     8761 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     8821 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     8881 ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag
     8941 cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca
     9001 gaaggtcgca cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct
     9061 caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt
     9121 ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac
     9181 cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag
     9241 atactcttcg cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg
     9301 tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac
     9361 ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata
     9421 atcgacaatc gaactggcgt cggtccaact gcggaataag caattgtttt taaagaaaga
     9481 gttatgtcca tagcaggcgt gtgccatcac cagcgcctgc atagtaatgg tgttctcttc
     9541 catcaagtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca gcccttgctg
     9601 accgtgctta tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata
     9661 gttaattggc atgccgacgc tggaataggc atccatcatc tgttctgagg ttatcacttc
     9721 aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag
     9781 ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt
     9841 attcatagaa tcgatcgtcg ccatacgcgc acctcattgt tgtcggcgct ctctgtgtgg
     9901 agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc
     9961 tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg
    10021 ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga
    10081 tgtgagtcag ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca
    10141 agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag
    10201 ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag
    10261 gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc agtgccgcga
    10321 atgccgggca aatctccccc ggatatgctg caccgtgggc agcaccaggt gtgcctttaa
    10381 aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc
    10441 agtttcagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg
    10501 aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc
    10561 gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta
    10621 ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac
    10681 cgtatcagct gctggaatcc agccgcctgg cggaagtgga tcccgcgctg gcagaagtag
    10741 cgcacaaact gacgggcggc ctgcagttac ccaatgatga aaccggagac tgccagctgt
    10801 ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc
    10861 ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagttt ggcgatgaag
    10921 tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag
    10981 gcattgttga tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc
    11041 aggaagatgg tgcgccagta tccaccattc ttgatgaaac ctacaaaatc gccattaccc
    11101 gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc
    11161 tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg
    11221 gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc
    11281 cggttgtcgg gagtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg
    11341 gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc
    11401 cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac
    11461 gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg
    11521 ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc
    11581 acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct
    11641 ggagcgcgct cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt
    11701 tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg
    11761 atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca
    11821 aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg
    11881 ggatgaatcg gttgggcttc cagtccgatc gcgtgcttac cgtctggcag cagttgcggg
    11941 caatggcgaa tgttggcgaa atgaccctga tgtcgcattt tgctgaagcg gaacatcctg
    12001 atggaatttc cggcgcgatg gcgcgtattg agcaggcggc ggaggggctg gagtgtcggc
    12061 gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc
    12121 ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata
    12181 ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag
    12241 cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca
    12301 ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc gcctaccggt gctcctgttt
    12361 tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctggcggttg
    12421 atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga
    12481 tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcac
    12541 tggcgttacg cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggaggcagcg
    12601 tcttctggtt gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga
    12661 cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg
    12721 aactcgccgc acaggcgttc tacataaaac gcttacgctt cattgttgac tcggagtcga
    12781 cttcagataa atcacgcttc acccttgatg cagattcggg ttcatgttca gattgttgat
    12841 taacaggctg cgagtcctgt tgtctttcca gactcaacag gcccaactgt tgatccagga
    12901 gctccaggtc ctcctgtacc ttcacgcgct ccaggagttc cgggtcatcc aggaactcca
    12961 gatccataag gtcctccagc tcctcgagtc cttccaggtc aacctagcct tccttgtgca
    13021 aaaagtccaa aaggtccttc aggccctgct gttttttcag gccctcgacg gctttgagca
    13081 gcgtcggtaa attgtctttg tcccacgggt taccattaca gtcaaacacc ttcaggctct
    13141 ggggctgcgg cacctgtacc gttttaaact tgttttctcc acagtagtaa attattagtt
    13201 tgggcggcag ccgtggtgaa gagatcaaat cgttactaga acagtcaaat actttcaggc
    13261 gagtcaagtt ctccagcatc tcatcaggta ttgagttaat attgttgccg gcacaggata
    13321 gtttatacag gtactggggc atcgacggcg gtaaaaattg caagaagttt tcagaacagt
    13381 taatcactct caggttagtc gacagctcag gcagccagat aatactgttt tcatagcact
    13441 tcagtaccat cagttttgag ggctgcacct tcggtaacaa gatcaagacg ttatctgtac
    13501 agtcaagcac cagcagccac tttggcatca ccttcggtaa cgcggtcaac acgtttttac
    13561 tgcagtaaaa ttcaatcaga tgtggaggca aattctcagg gagtgaagtc aatcccaggt
    13621 tgctaagatt gagcatactc cgtcttgtaa tgagacattc cttcattctg cgcaatgctt
    13681 ctgcccggtt ttcaatccgg cttgctggca ggttttcttc caggatattt tccttgattt
    13741 cttccagact ttctttcagg tcttcttggg gaatttctga ctgtttgttt tgctggtttt
    13801 cttccaggat ttcgtacagg tttttttgca ggttttcttg caaaccatcc tgctgtatag
    13861 cccattcgca ccatttggcg accatcatga actcgttatg ttgctgattc agacgagtcc
    13921 ccaacgtgat gatggtattg ttgtccacct gtatgttcac tttgcccgcg atactgaggt
    13981 caatgatctg ccagggggca tcaccccagc tgaatttttg cgggctgaga tacttcatgt
    14041 tctgcaaaat ttgattcttg agaagagccg ccctggcggg cgtcgtacgc ctgggttaac
    14101 ggaagatggg ggaatggcgg gggcgaatgg gcagatgtct ttaaagctag ccataatgct
    14161 acgtttcctt gaaataaata tcctctggca gcgccggaga cgtgaatgtg gtcaccgcaa
    14221 aggagccaca ggacgtcatt tgcgctgata tcactactca tcgttgactt ttttcctggt
    14281 cagaaatgcc gtgtcgggtc tggtacatgc tgtattacgg cctaggtagc tctgacgtat
    14341 taaagaaaat acgagagtga acgacccgga aataatcgtt attaagcaaa ggaataacta
    14401 taagaaatcg ctcatgcatc cggtgctaca ggcggatgaa acgcctattg tgtgggctat
    14461 gtcagtgcgg caggttgcgt gggggaatat gttcaggcga tgtcaggcag gggcccctga
    14521 tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa aaagccctta
    14581 cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa tggtttgtca
    14641 tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg aaatgacaag
    14701 tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag atgctgcatt
    14761 aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac acttcattat
    14821 cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca ccgacaaccg
    14881 gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta tcacgatatt
    14941 ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg ctggcttcga
    15001 gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg ctgaacagtt
    15061 taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg ccttcgcgca
    15121 gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct gcaatacgcg
    15181 tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta tcagcactca
    15241 attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca acgcgtgaca
    15301 ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac gagagtgatg
    15361 ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag aacagtcgcg
    15421 cattctccag ccccgccatc atcgggaaca cggctaggat gattggcacc gcgccgcgta
    15481 atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa gggagcaatc
    15541 cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa atgagcgccg
    15601 gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc aggaacatgg
    15661 cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag cggttgcgaa
    15721 tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg ctgccttcca
    15781 gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat ggatataatc
    15841 cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg ccaagaccga
    15901 tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa ctgacactgc
    15961 tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc attggatcat
    16021 tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc ttaccaccca
    16081 gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt aataagcctt
    16141 caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca gaggtgataa
    16201 gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag gaactggcct
    16261 gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt accatgtagg
    16321 cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc agcatgccga
    16381 tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa ctaagtaata
    16441 tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt gtggcatcca
    16501 atggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat tagcggcaga
    16561 gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac aggatgaccg
    16621 ctaatcgtca actgagcgcc ttcccgggtg ttattcagaa tggcatgtgc gcccaacggc
    16681 agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg aatcgacagg
    16741 cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata atcattgggc
    16801 gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat tcccgcatga
    16861 tagagctgca acagcatacg ttcgacgcgg aaagggtgca cgttaatatc ttccagcacc
    16921 agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat cagcatcgca
    16981 agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc ttgccattca
    17041 atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa ggcgttcagt
    17101 tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac attgccttgc
    17161 gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag taacgggtca
    17221 tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg actggcaccg
    17281 taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc aagggaattg
    17341 agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg ggcaataacc
    17401 tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg cagcgcggcg
    17461 tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat ggcaattcct
    17521 tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg tcacctcaac
    17581 ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc tttttgattg
    17641 tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg aacgcgaaag
    17701 ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt gcagcctggg
    17761 gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt aatccgaacg
    17821 cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc tccggacgtg
    17881 atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg aaaaatccag
    17941 agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc ccgctggcag
    18001 gcattgaaga tccgaaggta ctgcaatatg cgctggtggt gtcatacgct aacggggcag
    18061 gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa atcaacgatt
    18121 tggatgctga cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg caggctccgc
    18181 gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc gcactttgtc
    18241 cgctttttcc cgggcttctc gctcgagaga gaaaataatc cgctgtaatt gccgctccac
    18301 cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg tttcattctt
    18361 gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt gaaaaacacc
    18421 ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta attcggcagg
    18481 ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca agcggaaacg
    18541 taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga gtggggcgga
    18601 gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta cggtaataaa
    18661 tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact cgactttcgc
    18721 accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga tgttgtcttc
    18781 ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta ttttgctgat
    18841 cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca aatcgcgtaa
    18901 cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat gactcacgcg
    18961 gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac aaaacttaat
    19021 acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac gcccagcatc
    19081 gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga ccgattcatc
    19141 gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg atagccggca
    19201 ttatcggcaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg acctgtattc
    19261 tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc attgacggct
    19321 ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct atcctggtgc
    19381 tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat agcgatacag
    19441 atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga aattgcgacc
    19501 ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat tgaccgacgc
    19561 attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca ccgcaaaacc
    19621 ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct cttccgacag
    19681 cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg tctgcgttgg
    19741 aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca gagcgtagct
    19801 caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca gaccgctgta
    19861 atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt gcgagaggat
    19921 ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc gcgtgaaggc
    19981 accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgcg ggttaacaat
    20041 cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg tcggaaagcg
    20101 tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt atttccccat
    20161 cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc tgccatcttt
    20221 ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg ccacgacatc
    20281 gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac gatcttcata
    20341 gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt tccagttaac
    20401 ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc cggcaccgtc
    20461 gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag ccagcgagtt
    20521 gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca gggtaaaacg
    20581 gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact ctggccactg
    20641 ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt tttcaccatc
    20701 ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac cgagcttcac
    20761 aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc ctgcaatatc
    20821 attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac cgctgacttg
    20881 caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg cattgtcctg
    20941 actacggctc catccctggc gatatcccag acttgcttct tcagggtggt gatattaatg
    21001 gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga aacttcgact
    21061 gcccccagat tgttggtcag aatccgcgcc agatcgaggt tgccgtcata ggggacataa
    21121 atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg aacttttacc
    21181 tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac acttaatgcc
    21241 tgagcgacat tctgtttatc caaggccttc atggtgggtt gatcgagaac ggtggtcgtc
    21301 gtcgatgata ccggactgga ccatacggtc agcgtgtcgc tctcctgagc cgctacggcc
    21361 tgctgggtaa aggcaagtga taatgctgtg cagagataac gttttttgag tctcatagaa
    21421 taaattccgt ttaacaaaga attaaataga ggctgtgggt tgcagactga ctttaatgtc
    21481 agctggtgcc gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc agtcgaggta
    21541 atttttgagt gtcgtcagat taattccgcc ggtcagagcc agcgtgcagt gaggagccag
    21601 cgacggcgct atttgcgcaa tttcagttgc ctgttgcgga ctaaatttgt cgagctgaag
    21661 tacgtctggt tgtgcgcgta acgcggcgat agcctctttc ggcgtgtcgg cttcaacaac
    21721 aattttcttc tcgggtgcgt ggcgacgtaa ctgagcgatt gcgcctgacc agccctgatt
    21781 gtcatgaaga aaatggcggt ggttggcaaa caataatatg gtttccgcac atccggcacg
    21841 atgaattagt cctccggcag ccagaattgc ctgcgaagcc agtaagcggg tgcccggaat
    21901 tgcttttcgg gtgcaggcga tattgccgtc aggataacgt tcacgaagca atgccagcat
    21961 ttgagcgaga taatcagaaa caccgcaact ccactccagc acattctgga ctgccttcca
    22021 tccctgatga agtgctgcgg cattaccctg cgcacggatt agccgttgac ccgcgttcgc
    22081 ttgtgaaccg tcgctgaccg cgtcatcaat agttaatccc agcgtagtta acatcttaca
    22141 cgcgacagaa acgccgctga cgcagccgcc ctgacggtga aaaaacgcta tatagccagg
    22201 ctggtgtcca atatttaatg ctcgcgtggt caggtcacct ccctggatat cttccagcag
    22261 taacgcatcg atttgtgcct gagaaaggaa aatcatcaca gtgcctcctg cggtacgcta
    22321 tcccaccaga tgagcgccca gtcgcggctg gcaggggcga gggcacgagc atcttgttgt
    22381 tgataccagt gatataaccg ctcacgctca tcgtcgttaa gcgcgccaag actccagctg
    22441 acgttctgct caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg tccacggata
    22501 aaatcaacgt gggcataaat tcccatctga tacaggacgt tgagcgcaaa gatatagttg
    22561 ggtaattcga tcacttcccg accagccgcc cgctgaatgg ctggagagac gaacgaggtg
    22621 ctgaccagat gagttgtata gacgcgaagc cgtgcctgat tattcagttt gctcattgca
    22681 tggcgcatat cagccaccag agtggagcgg gaagccacgg caatatcgca gcgtggcagg
    22741 tcactccagt cctcttccca ggcgcgctgg atccagtgga cattgtcagc cttcagtgcg
    22801 gcggcgcggc gggcggcaac gttcagcatc ccctgactgt aatcaacgcc ataaaccgta
    22861 gtgagtttgt cagccagcgc cagcgatacg gtgcctggtc cgcagcccat atcgaataag
    22921 gtctgagcgc cttgcaggtc aattttagcg atcagttgtt gcaggtagct gtccgttggc
    22981 gaggcgcaat tttccgccat tttctccgcc cgttgatccc agtgatcggg cgttttttct
    23041 gtgcggtgag ccagtttgag ttgctgcaga taaaggtctg caaaatcaat atcatcaatg
    23101 agcatgattg ttccttagcg gctaattgca gagaggtgat gatgaatttg gtcggcagaa
    23161 acccgataca gggccgccag tttgttcgtt gttaactgtt cggtcggtaa gccttgtgtt
    23221 actcgtccat ctggctctac ttggataatg ctgtcggcaa tggcgttggc atggagcgta
    23281 tggtgagtcg acattaacat tgtcatgccg ttattttt
//
LOCUS       DAZSNM010000045        23026 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZSNM010000045 DAZSNM010000000
VERSION     DAZSNM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23026
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.045"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            376..815
                     /gene="ycgY"
                     /locus_tag="ACHW42_001490"
                     /pseudo
     CDS             376..815
                     /gene="ycgY"
                     /locus_tag="ACHW42_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
     gene            complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHW42_001491"
     CDS             complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHW42_001491"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR8943365.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACHW42_001492"
     CDS             complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACHW42_001492"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFR8943366.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACHW42_001493"
     CDS             complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACHW42_001493"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFR8943367.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACHW42_001494"
     CDS             complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACHW42_001494"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFR8943368.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6281..8200
                     /gene="dhaR"
                     /locus_tag="ACHW42_001495"
     CDS             6281..8200
                     /gene="dhaR"
                     /locus_tag="ACHW42_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFR8943369.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(8300..11167)
                     /locus_tag="ACHW42_001496"
     CDS             complement(8300..11167)
                     /locus_tag="ACHW42_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR8943370.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(12011..13102)
                     /gene="ychF"
                     /locus_tag="ACHW42_001497"
     CDS             complement(12011..13102)
                     /gene="ychF"
                     /locus_tag="ACHW42_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR8943371.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(13219..13803)
                     /gene="pth"
                     /locus_tag="ACHW42_001498"
     CDS             complement(13219..13803)
                     /gene="pth"
                     /locus_tag="ACHW42_001498"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR8943372.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            14081..14359
                     /gene="ychH"
                     /locus_tag="ACHW42_001499"
     CDS             14081..14359
                     /gene="ychH"
                     /locus_tag="ACHW42_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR8943373.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(14414..16093)
                     /gene="dauA"
                     /locus_tag="ACHW42_001500"
     CDS             complement(14414..16093)
                     /gene="dauA"
                     /locus_tag="ACHW42_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR8943374.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(16218..17165)
                     /gene="prs"
                     /locus_tag="ACHW42_001501"
     CDS             complement(16218..17165)
                     /gene="prs"
                     /locus_tag="ACHW42_001501"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR8943375.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(17316..18167)
                     /gene="ispE"
                     /locus_tag="ACHW42_001502"
     CDS             complement(17316..18167)
                     /gene="ispE"
                     /locus_tag="ACHW42_001502"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR8943376.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(18167..18790)
                     /gene="lolB"
                     /locus_tag="ACHW42_001503"
     CDS             complement(18167..18790)
                     /gene="lolB"
                     /locus_tag="ACHW42_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR8943377.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            19004..20260
                     /gene="hemA"
                     /locus_tag="ACHW42_001504"
     CDS             19004..20260
                     /gene="hemA"
                     /locus_tag="ACHW42_001504"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR8943378.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            20302..21384
                     /gene="prfA"
                     /locus_tag="ACHW42_001505"
     CDS             20302..21384
                     /gene="prfA"
                     /locus_tag="ACHW42_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR8943379.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            21384..22217
                     /gene="prmC"
                     /locus_tag="ACHW42_001506"
     CDS             21384..22217
                     /gene="prmC"
                     /locus_tag="ACHW42_001506"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR8943380.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            22214..22606
                     /gene="sirB2"
                     /locus_tag="ACHW42_001507"
     CDS             22214..22606
                     /gene="sirB2"
                     /locus_tag="ACHW42_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR8943381.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            22610..>23026
                     /locus_tag="ACHW42_001508"
     CDS             22610..>23026
                     /locus_tag="ACHW42_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HFR8943382.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLI"
BASE COUNT         5649 a         5756 c         5838 g         5783 t
ORIGIN      
        1 cattttatta atgcatttct cattcgggtg aaatttccgc tacctggacg cataaaaccg
       61 gaactctcgc caaaagtcgg gcactccgaa ggaattgacg cgttgcgctg ctctgtaacg
      121 gcatttaccg caagccattt atttcgccag cgacaatccg cagagaacca aacgcatcag
      181 gaaacgactg aacacaataa atactgtatt gatcaacata ttatactggc acctccagca
      241 cgcagtgagg atacactgta tcatcagcga atcttatcac tgcggagctg gttgaacgac
      301 tttctgagcg aaataagtcg gttcccggta acaacaacag aatccttttt taattattgt
      361 ttcgttgttt ttggtgtgat tcagaaaata aatattttag acagtagcca gtcaggcgtt
      421 aaaaatgggg cggatagtga ggcggttttg gctgcgctta ttgaacattt agaattgatc
      481 aatccttctg gcagattaag ccaaaatacc cgttcggcaa tgcttcagtt acgcgaagag
      541 gaatggtctg aattcttttt ttggttgcta aattctctgg agtgtctgga ttatgtgatt
      601 attaatttaa ctccagaaag caaaaaaacg ttgatgagtg aacaccgtaa caatattcgg
      661 tagcaattga tgctttatat agccaacgtc ggcgtaaatc gccgggtgat gaaagtgaaa
      721 cacttacccg tagaaatgac gctatcttcg gcaaccacgt atggcaaaca tttgcgcaat
      781 acttcccgcc aggacttgaa aaaccgtctg tgtagaaaaa accatagata tggtgttatc
      841 tgccgacaac gccatccgga gaagccgaat gacgttgtcg gagtaagcgc tggttaaggt
      901 gtgggttgtg cctctttggt tgagggttgc gtcgttgctg acttaacggt cggatgcgtc
      961 gccggaacat tgtcacacgg ttgctctttc gggcagatca aatccagcat tttcagcgtc
     1021 acgccattgg tccagccaaa gccatcctgt aatggatatt cgccaccgcc gccccccgtt
     1081 ccggtggtgc tgacatcata tttttccacc agctttttct cccggtcata ggtgtgctga
     1141 acattggtca ggaagtgcca gctaatgtcc atcgccacct ctttttgccc gtagttttgt
     1201 aatccttctg tcgcgaccca ctgtaacggt gcccagccat ttggcgcatc ccattgttgc
     1261 ccacttttca ccgacgtggt gttcaggccg ccgggttgca gcagatgtgt tttcgtcgcc
     1321 gtcgccattt tgttggcgcg atctttcgct gccgcattga cgtacagcgg gaacagggcg
     1381 gccgcggtta actgattgcg cactttatga cttttcaggt cgtaatcggc ataccagcct
     1441 tgttgatcgt tccacaggta tttttcgatc cctttttgac gggcattcgc cagcgtttcg
     1501 tactggtttg ccatcgcgtt atctcctgca gctttgctgg cgcgggcgag gattttttcc
     1561 attttaaaca tcaggctgtt cagatcgacc ggtacgatgc tggtggtgcg taaggtattt
     1621 aactgctgcg ggttgtccat ccagcgcgag ctgaaatccc agccagacgc agcggcagag
     1681 cgcaggtcgc ggtaaatttc agtggcaggt cgattcggat tgcttttggc ggtggcaata
     1741 tcttccaccc atgactctgg tcgtggcgta tcgcgatcgt cccagtagcg gttgagaagg
     1801 gtactatcct gaagttttac aacgcgtttt tcctgttgtc cggcttgcag gttttcaaca
     1861 ccgtccatcc agtaagcata ttctttttgc atttgcggca ggtattgctt caacgcggca
     1921 tcgccttcat gctgcgccag taactctacc atcagggcaa agaagggcgg ttgcgagcgg
     1981 cttaaatagt aactgcggtt gccgttggga atatgaccgt aagtgtttat ttcatgagca
     2041 aaattggcca ccatatccgc gattttatcc cagtgaccac tttcggcaag tcctaacatg
     2101 gtgaagtaat tgtcccagta atatacctcg cgaaaacgtc cgcccggcac gacataaggt
     2161 tccggcagcg gtaacagaga atcccatttt tcggtgtttt cggtagaacg cgttaatacc
     2221 ggccaaagtc cgtcaatatg ttcgcgcagt gactgcccct ctggcggaac atatttctcg
     2281 ccttctttcg gcagggtgaa attgacgtta acgaaatggc gcagatcaaa tccgctctgg
     2341 ttttgctgca tccgataatc agcaaggatc atcagcggat cgctgttagg cacggcatcg
     2401 gcaaaggttt tttggtccgg aaaaagtttg acgttttgca catcattaaa cagcggccct
     2461 aataaaatat caggcggctg tggtgttacc ggtgtttctt ctgcctgcac cgatagcgca
     2521 gcgaaacaca aaaagataca ggctggaatt aacgccattt tttgcgggcg agaaggtgcg
     2581 ggggatttca tcaatcattc tcctttggcg aaaccgaata aaacggtatt caagtatcag
     2641 aaaaccttag ttcaggatcg cactagctgc atgatcgaaa tctcattctg cgaacggtca
     2701 ggcattttag aaaagggaat taaagggata aataacggcg aaatagagtg atcaaataac
     2761 gtctgtttat tagccagcca gcgacatttg gttcgcgatt agcgaagaaa aatcatcata
     2821 aattggtaaa aaatataaca ggtagttaaa aaacatcagt gctgggtaaa ttgccggatg
     2881 acatcagaac gatgccatcc gaacggtggc ttaaccctga cggttgaaac gttgcgtttt
     2941 aacgtccagc gttagcgttt cttctggttg tatcgcatac agtttctcac cctgctggca
     3001 aatccagcca atccccagtt cacgggcgat tagggcgctg tgggataccg gacttccggc
     3061 gctaaggcag atacctttta caaccgccgg atccagttgc agtactgtgg aaggataaat
     3121 gttctccgcc agtagaatag ttggcgagtt aaactgcggg agttcttctt tcgtttgggt
     3181 caggtggacc agggtgcgat gcagaagatc gtccacatca atatagcgag cttgtagata
     3241 ttcatcatcc agttgctggt attgctggct aagttcttta agaacttgct gccaggcata
     3301 ttctgccgtg caatgttcat gctgaaggag ttcgcttgcc gccgccagca gttccggatc
     3361 acctaacagt gtatggtgac cagaaaagat tgcggcaata tcgtcaagcc cgctggcttc
     3421 tgctttcgct gttaacgtca tcagatctaa taacgtgaag tcaatagcct ggcgtaatcg
     3481 atcttgttct tcttccgcgg tcagggttga ttttgcctgt accgtacata aaactggttg
     3541 ataataaaag gctttacccg aaacaggcag aacgggacgc agagtaggtg gagcgacttc
     3601 ctccgtttca ccaaagttat cttcagccag gtgacggaaa gcgatcagtg cctcttcagc
     3661 ttctggccct ttcgcaatca ggcgcagcgt atcgttatag cgaacttgta gtaacgcaat
     3721 ctggttaata ctctctggtg tgacgcattt gccgtttttt tccagcaaca tatcggcatt
     3781 aaatgtcgat aaggtataaa ccagccggga ggccggacgt acatgcaggc cgttacggtt
     3841 ttttatgacc accgccagag aacgggcttc ttcatcgtac ggaggacatg tgtcagagat
     3901 ttcagtgtcg gaggacggta aacccagttg ttcacgtttg gcttccagcg catgcatagc
     3961 gtcaaagata actttgtcga tatccgcccc cgaggccgcg ctgaccgttg ctgccagtgt
     4021 accttcgacc aacggcgcag cacacaaacg tacttttgcg gcgatctcgg gagccagcaa
     4081 ttccagcgca gtttcagcac tcaataatgc gctacccata tccatcatga ccagcacatg
     4141 gtcggcatca gcaacagatt cgatggcctc catcactttg acggcatcgg taccaatggg
     4201 attttgtgga tcgtcaattc ccgcggcaat ggcgatttta caactatcac tcattaacat
     4261 ctgacgggct aattcaccga caccttctcc cagtcggctg ctatgtgaaa ctatgaccag
     4321 gtttaccatc accaattcct tactcttttg cggctaacgc caacatttgc atcataaaca
     4381 tcaccgaggt cgcgccggga tcctggtgac caatactgcg ttcaccgaga taactggcgc
     4441 ggcctttgcg ggcttgcatc gtaatcgtac tttgtgcagc ggattcggcg atgctactgg
     4501 cagcttcgag cgccaccgga acagagagat tttgctcgct ggactgacgt aacgattcca
     4561 ccaccggcac ccacacatca cacatggttt tatcgccagg ttcggctttc ccgcgactga
     4621 ttacgccgtc cgcgccatcg cggaacatct gataaagctc ttccagtgtc aggctttgcc
     4681 gtgcctgggt cgcctgtgcg gcgcggataa agaaggtacc gaacagcgga ccactggcac
     4741 caccgacgct ggaaagcagc gtcataccgg tatttttgag aatgaaaccg atatctttat
     4801 ctgcgatagc agggagtttt tccaccactt tgctaaagcc tcggttcata tttagcccgt
     4861 ggtcagcgtc gccaatctcg cgatccagcc cggtaagata ctcgctctcg gtgttgaaaa
     4921 tatcgccaca acgagtgagc cagttaacaa tttgagttct gctcagtgac attgctttct
     4981 ccttatttac cccagttaag ggccggggtg tggaccgggg cgtcccagag agccagcgtt
     5041 tcgtcatcaa ctttcagtaa ggtgattgag aaaccggtca tatccagtga ggtgcagtac
     5101 gcgccaatta aattacgttc gatagtcaat cccgcttgct ggcaacgtgt ggtcaggcgg
     5161 ttatagacgc cgtacagctc agaaagcgga gttgcgccaa gattgttaac cagtgcaatc
     5221 acccgatcgc cagactggag cggttgtttg gtttgtggtt cttcctgcca gctgccttgt
     5281 tgataatccc agaaacgcaa agtgcgatgg tatgagccat gttccagcag ggtgtcgaac
     5341 atttcatcga cggtttgatc aagggaagag aaagggcggc ggtcaatacc tggctcacca
     5401 tgaatgccta cgccaaactc catctcatta tccgcaaggg taaaagaagg tttgcccgcg
     5461 gcaggaacgg tacaggcacc gagagcgata cctattgagt ggccttgatt attcaactta
     5521 cgccccagtt ccgcacaggc gtccagtgag tcgccacgct ccgccgctgc gcctacgagt
     5581 ttttcaatta atacggtgtt ggcaacgccg cgtcgcccgg cggtataaag actgtctttt
     5641 accgcaacgt cgtcatcaat gaccacagtg gtcactttta cgccgctatc gtgcagtaac
     5701 tcggtcgctg tttcaaagtt aagaatatcg ccggtgtaat ttttgataat caacagtaca
     5761 ccttcgccgc catcaatttg catggagcat tcaaagattt tatcgggcgt cggtgaggtg
     5821 aaaatttcgc ccggacaggc ccccgaaagc atcccctgac cgatataacc acagtgcatc
     5881 ggctcgtgtc cgctgccgcc acccgacagc agggcgactt ttcctgcaac aggggcatca
     5941 gctcgggtga catacaccgg atcctgatgc agtgtcagcg atggatgcgc tttcgccagt
     6001 cctgccagtt gttcgtccag tacgtcttgc acatcattga tcaatttttt cattattttg
     6061 ctccagcaat tacggtaggg catggatgat gttcaacgac acggcgacca ctgactgccg
     6121 atgaatccat tgtgcatcag gcaagggaaa agaaaattag cccaaattat gtttcatagt
     6181 gaaacatatg ctttaatgaa tgttccatat tgaaactttt acgtgtatta atacttaaaa
     6241 ttgcgagccg gaacaccttt tgtcataagg gatgcgggat atgagtggcg cttttaacaa
     6301 cgatggtcgg ggcatatctc ccttaattgc aacctcctgg gagcgatgca ataagctgat
     6361 gaaacgggag acatggaacg taccacatca ggcccagggc gtgacatttg cttctattta
     6421 tcggcgtaag aaagcgatgc tgacgctcgg gcaggctgcg ctggaagatg cctgggaata
     6481 tatggcaccg cgagagtgtg cgctgtttat cctcgatgaa accgcctgca ttctcagccg
     6541 taatggcgat ccgcaaacct tgcagcagct aagtgcactg ggattcaatg acggcacgta
     6601 ttgcgccgag ggaattattg gtacttgtgc gctatcgtta gcggctatct ctggtcaggc
     6661 cgtgaaaacg atggccgatc aacatttcaa acaggcactc tggaactggg ccttttgtgc
     6721 aacgccgttg tttgacagca agggccgatt gacgggaaca atagcgctgg cgtgtccggt
     6781 tgagcaaact accgcagctg atttgccgtt gacgttggca atcgcccgcg aggtcggtaa
     6841 tttactgctg acggacagtt tgctcgctga aactaaccgt catttaaatc aacttaatgc
     6901 cctgttagaa agtatggatg atggcgtgat tagctgggac gagcagggta atttgcaatt
     6961 tattaatgcc caggcggcgc gggtcttgcg ccttgacgcg acggcaagtc agggacgggc
     7021 aatcactgaa ctcttaacgt tacccgccgt attgcaacaa gcaataaaac tggcacatcc
     7081 gctcaaacac gtagaagcaa cctttgaaag ccagcaccag tttattgatg cggtgataac
     7141 ccttaaaccg ataatagaaa cgcagggaac cagctttatt ttgttgctcc atcctgtgga
     7201 acagatgcgg cagttaatga ccagtcaatt aggaaaagtc agccatacct tcgctcatat
     7261 gccacaggac gatccgcaaa cccgccgctt gattcatttt ggtcgccagg cggcgcgcag
     7321 tagctttcct gtcctgcttt gtggagaaga gggcgtgggc aaggcactgc taagtcaggc
     7381 aattcataat gaaagcgagc gtgctgcagg tccttatatc gccgtcaatt gtgagttata
     7441 tggtgatgct gcgctggcgg aagaatttat tggtggcgat cgcacggaca atgaaaatgg
     7501 ccgtctgagt cggctggaac tggcacacgg cggcacgctg tttcttgaaa agattgaata
     7561 tctggcggtg gagttacagt ctgctttgct gcaggttatc aagcaggggg ttatcacgcg
     7621 actggatgcg cggcgtttaa taccaattga tgtcaaagtg attgcaacaa cgaccgcgga
     7681 cctcgcaatg ctggtggaac aaaatcgttt tagtcgccag ctgtattacg cgctgcatgc
     7741 atttgaaatt accatcccgc ctctgcgtat gcggcgtggc agcattccgg cgctggtgaa
     7801 taacaaatta cgcagtcttg aaaaacgctt ctctacgcgg ctgaaaattg atgacgatgc
     7861 cctcgctcgc ctagtttctt gtgcatggcc aggcaacgat tttgaacttt acagcgtcat
     7921 cgagaatctt gctctgagta gtgataacgg gcgcattcgc gtcagtgatt tacctgaaca
     7981 tctgtttacc gagcaggcga cagatgatgt cagcgccacc cgcctttcca ccagtctgtc
     8041 atttgcggaa gttgaaaaag aggcaattat taacgcagcc caggtcacag gcggtcgcat
     8101 tcaggaaatg tcggctttac ttgggatcgg ccgcactacg ctgtggcgga aaatgaagca
     8161 acatggcatt gatgcagggc agtttaagcg ccgggtatga aagacagaaa cgatttctga
     8221 tacatcagag tgatctgtat ttcattccgg cgcacgctaa caattttcag catcgtttaa
     8281 gggcttgtct atcccgcact taaaagctga agcgatatcc tccgttgact tgcttctgat
     8341 caaagaggtt accctgcgtg taatccgctt cgagatagaa tgtgtgctgt ttgttatact
     8401 gtgcactgac tcccacgccg ttattccagc cattaccttt gaaactgtac ttctcccggg
     8461 agtcgttcaa cagatattcg gtatcccctg aaaactcgcg gatagcgcca gtcttcacat
     8521 agacattcag ctggctgttg cctgcggtga tgtcataccc cagtatcatg ctggcacgcc
     8581 ccagcagcga ttcgtagtga ttcagatgta tattgaggcc attactcgcc ttcatagcca
     8641 tctcattctg gtggctgtat gtaagctggg tttgcggctc tatatagaac ccataaccag
     8701 taggggacag gttgaacctc tgcccggctt ccagggagat gctcattcca ttcgcagtgc
     8761 cgttggcgtt aacgccgttg ttctgactgt ccagtacgtg gaaactattt ttctggcgcg
     8821 atgcttttat aacgagatcg ctgtaaaaac cgttttgtgc catgtaactg gcgtacattc
     8881 ccatgtagtc tgaacgtgcg gtaccgtcgc ctccgctata gtccggcgat gcatgtgttg
     8941 agtcaatata cagaccgaca tacaacggca ttacatcaga gagacgttta tccccaccaa
     9001 actggatacc gctgtaaccc atgtcaaagc cgctcagttt gccactggca aaggagtcca
     9061 ggcttccccc ataactgcgc aaccagatat taccgtcttt actctgattt cgcaagtcac
     9121 ccatccgttg catcagcgta cggttttcaa catagttcaa taagtaaccg acattgagat
     9181 aattgccgcc agcatctgcg gtcgtggtgg gtttaggcgt gggagcgggt tcaggcgtag
     9241 gatcggggtt gactatggga ggctgagcgg gagctggtgt gggttcagga ttaggagtcg
     9301 gttccggaac tgtcccggaa gcgtaaagct cccagttagt gccattttta cgcacatcgt
     9361 acagatatcc ccccaactca acctgagaag acgcgctgaa cgaggccgcg ccgtcagtgg
     9421 tttttaccac tgtcagaact tcatttcccg ttgtggcctc gctgccctgg ttgcggatag
     9481 ccaatacgtg attaccagca ctactcccgc tgatattcaa taaatcccct ctattattaa
     9541 cgccattacc ctcgccaaca acatcagcac gcataataaa ggtactgtta ccgctcaggt
     9601 tctctacgtt taatgtggta aatgtgccgg cagttgaccc gtggctggca aaatcgacag
     9661 ttgaatggct cagcgccagc gtgtcgagat tagaattact tgttacgttc cagacgctgt
     9721 tattcattgc aacgtccagt ttcccgccat tgacattatc gctgagggag gaacctgtcc
     9781 aaaccgaccc agggtgcata tccagattga ttagcccacc tttggaaaga acgctaccgt
     9841 tgataagcat acgacccgag gcgttgatgc ggctggcggc ataaccatcg tcatgttgcg
     9901 ttgcgatggc catctggtcg ggtgtactca tatcaatgac cagatcgctc gtgaggtcga
     9961 tctggctgtt ggtcatggca taaatccctc tggctcctgc cgcgccggtg atagccaaac
    10021 tgtctccggt tattctaccg ccgctgagcg cccacaaacc cagcgccaga ctgccattac
    10081 gatcaaccgt aatatcggta ttttgcatgt tgataacagc cgttgccgtc tgggccgagg
    10141 caccataaga accgccggaa aagatgctgt ttcgttgcgc tgccgtgcca gaaaaattga
    10201 ttgtcgcgtc tgaactactg gtgacgaggc caccgccctg cgcggaagaa atatggctat
    10261 ctgcaccgat agtggttgta ccaccacgaa cttcgacgcc attggccgcg gctccagtta
    10321 catcaacagt gagtgcattc gcgctaacct ggccaaagct ccagaggccg tgagcattat
    10381 cgccattggt tttaatggta ctgtttgttc cgagatcgac aacagagttt ttctgtacgt
    10441 ttatccccat ggcgctgtag ccctgaacat cgattgtcag gtctgtcgcc gtaaaacgcg
    10501 cagcaccatt ggcgttattg ccgttgagac caccgatata aacacctgta cttccatcgg
    10561 tcttgatttt acttccgctt ccaagatcga cactggtgcc atagtcattg atggttaggc
    10621 ctataccgtt cgagttttca atggtgaatt gagtggctgt tagtgttgag ctatgcccaa
    10681 taatgatgcc gtcatcgtta ctcttaatgg tgctgcctgt gcctaagtca gcatgggtat
    10741 agtcaccaat taagttaatg ccgatggcag aggtttgacc aacaacatct actgtgagtc
    10801 ggttagctgt cagggagctg tttttgccct gaataatgat tccttttgca tatccagagg
    10861 cgtcgttgac attgacggtg acatcattac ccaggttgat attgccaggc gtgtttcctg
    10921 ctggggtcat tacaccatag aggtttcctg atggatcggc ggtatctcct gtaattctgt
    10981 caccatcatt aatagtaata ttggtgccat attgacttcc tgtatcagca gctatcagca
    11041 ggggggaaaa gataattgca ttcaaaccaa tagcgccaaa ttttatcagt tgaattgagg
    11101 gcgaacggat ttttaattca gcgagacgat cggctttggt attcccattg tgttgtttga
    11161 tgcccatacc actcctatat agtacccagg ctatgggtct atggattttg tcattttgtt
    11221 taagtcaatg aataatatcc tcttatcatc atgatatctg tatggtgcaa tcatgatgag
    11281 cttctctgag aataattctc tattaccggg caaaatcagg aacacaggaa ttaatatata
    11341 tttaaccccc cccttacaaa ggtgagttta tatcgagtta ttccctaagt tgtcgttact
    11401 tattccaatt cgttactatg aatatggcaa aaatattaca acaaacagcg gttaaaaaat
    11461 ttggtgaaga ttttttgtaa taatcctaat tagttatggg attttcgctt gatgtatctg
    11521 cataacaaaa acaatatgcg ccatttttgc attactaata agaaaaagca aaaaacatcg
    11581 atattcattt ttgttcaatg gattttctgg cctatcgtta ttattgtgct actggttatc
    11641 attttcatct tgccaggatc gcacgtaatc acgttattcg gggactttat ctgcgggata
    11701 ttacccttca ggtaagtcct tttctcgcca gcatattgga gcgagatcaa tattgatcag
    11761 ctgtgaagag ggctgatgtg tagtggaaca aaaactgacg ttatatcact gctaatgaga
    11821 tatcagtcat ctgaacactt atgtctttaa ttattctcgt ggttcactgt aggcaataag
    11881 cacaaaagtg taggatgtta caagaatgat taggactcgg tgaaatgaaa aatccacgca
    11941 attgcgtgga ttttatatac ttttgcgctc ttcatgagat ttagcgaaac ctcatgagac
    12001 aataaattaa ttagacgttg aaaaggaagt tcatcacatc gccatctttc acgatgtaat
    12061 ctttaccttc tgcacgcatt ttgcctgctt ctttcgcgcc ttgttcacct ttgtaagtga
    12121 tgaaatcttc aaacgagatg gtttgtgcac ggataaagcc tttttcaaaa tcagtatgga
    12181 ttttgcccgc tgcctgcggc gcggttgctc caaccggaat ggtccatgca cgcacttctt
    12241 tcaccccagc ggtgaagtaa gtttgcaggt tcagcagctt ataaccggca cggatcacac
    12301 ggttcaggcc cggttcttcc agcccaagct cctgcataaa ctcgtcacgt tcttcgtcgt
    12361 ccagttcggc aatgtctgct tcaacagcag cacaaaccgg aaccacaaca gaaccttctt
    12421 tcgccgcgat ttcacgcacc tggtcaagat atgggttgtt ttcaaaacca tcttcgttga
    12481 cgttggctat gtacattgtt ggtttcagcg tcaggaagct caggtaacga atagccgctt
    12541 tctcttcagc gcttaaatcc agcgcgcgca gcatacctgc gttttccagc tggggcaggc
    12601 atttttccag gaccgccagc tcagctttcg cgtctttatc gccacctttg gctttcttct
    12661 gtacgcgatg aatcgcacgt tcgcaggtgt cgaggtctgc cagcgccagt tcggtgttga
    12721 taacttcaat atcgtcagcc gggttaactt tgccggaaac gtgaatgatg ttgtcatttt
    12781 caaagcagcg aacaacgtga ccgatcgctt cggtttcacg gatgttggtc aggaactggt
    12841 tacccagacc ttcgcctttc gatgcgcctt ttaccagacc ggcgatatcg acaaattcca
    12901 tggtcgtggg aagcgtacgt tgcggtttta cgatttcagc cagttgatcc aggcgagggt
    12961 caggcattgg tacgacgcct gtgttcggct caatggtgca gaatggaaag ttggccgctt
    13021 caataccggc tttggtcagc gcgttgaaca gggtagattt cccgacgttg ggcaaaccga
    13081 cgataccgca tttgaatccc atgatttaaa tcaccttaat atcttaataa tcaacctgtt
    13141 attgctaaca gattgcagaa atggaaataa ctttgcctat tatacacggc actcggcaaa
    13201 aatgccgcag acaacgactt attgcgcttt aaaggcgtgc aatcggttcg ttgctttggt
    13261 caagccatct gtaaaccaca tttcagtaca acgcgccgct tcgtcgatgg cttcatcaat
    13321 taacttctgt tcactaacag gcggtttgcc taacacaaaa ccgacaactt tatttttatc
    13381 gcccggatga ccgattccga tgcgtaaacg gtgaaagtta gggttattac ccaatttact
    13441 gatgatgtct ttcagtccat tgtgaccacc atggccaccg cccaacttaa atttggcgac
    13501 gccaggaggc agatccagtt cgtcgtgggc caccagaatt tcgtccggat taatgcggaa
    13561 aaaactggcc atcgccgcaa cggctttgcc gctgagattc ataaatgtag tcgggactaa
    13621 caggcggaca tcttcgcttc caagggtgac tcgcgaagta taaccaaaga atttagcctc
    13681 ttcgcgcagc ggagcgcgca aacgctctgc cagtaagtca acgaaccagg caccagcatt
    13741 atgtcgcgtt gcggcgtatt cagcaccggg gttcgccagg ccgacaatca atttaatcgt
    13801 cacgtttttt tgtcctgagt gtgtacataa ctggcgcgta gtttactggt tgcggccccg
    13861 cttgacaaaa aactgcgtat caaatgcaga taacgtaata attgcctgag tggactatta
    13921 gaaagtcaag gtgttcaggc gtttatttgt aaagttttgt tgaaataagg gttgtaattg
    13981 tgattacgcc cgcacataac ccacagggtg ttgtctatac tttacacata aggaagaggg
    14041 gtattccctg ttacaaccca gaaagttccg gaggtgacat atgaaacgca aaaacgcttc
    14101 gatactcggt aacgtgctca tggggttggg tctggtggta atggtggtcg gcgtggggta
    14161 ttcaatcctc aaccagttac cacagtttaa tatgccccag tatttcgcac atggtgcagt
    14221 gctaagtatt ttcgtcggtg ccattctctg gctggcgggt gcccgtgttg gcgggcatga
    14281 acaggtgtgc gaccgttact ggtgggttcg tcactatgac aaacgttgcc gccgtagcga
    14341 taatcgccgt catagctaac aaataatgca gtttgctgac cagtcagttt gcgctgactg
    14401 gtcaatctcg tacttataaa tccgccatcg ccgcgcgacg attcgggaag aacgcgaggc
    14461 gtcccgggat cggttgaatg cctgcgcgag ccatagttcg cagtggctgg aattccacgt
    14521 tgcacacgcg cagttcacat ccttcgggca gacgcttcac aaaacgctgg aacgcatcaa
    14581 gaccaccagc atcaagtacc ggaacggcat cccacttcag aatcacaatc cgtttgcctt
    14641 caagacgtga ctccaggtcc gtgaataagc cttcagcagc agcaaaaaac agcgggccaa
    14701 taacgcgcag aaccaggaca tcgtctggaa catctacgac taccggtgcc aggcgagtca
    14761 tacgtgcgat acgacgcata aacagcagcg atgccagcac gatccccacg ctgatggcaa
    14821 taaccatatc aaacagcacg gtcagtgaca tacacagcag catgacgatg atgtcatctt
    14881 tcggcgcatg acgcagcaag tcgaccactt tgtgcgcttc actcatgttc cacgccacca
    14941 tcaacagcag ggctgccatg gcggaaagcg gcagccagga gagcagtggt gccagtacca
    15001 gcagggcgag aataaccaga atagagtgga tcaccgccga gataggggaa gttgccccgg
    15061 cacggacgtt agcggcagaa cgcgcgatgg cagcagtagc ggtaatacca ccaaagaatg
    15121 gagcgatgat attccccagc ccctgaccga ccagttcact attcgccttg tgcttagtcc
    15181 cggtcatacc atccagtacc acagcgcaga gcagagattc gatcgccccg agcattgcca
    15241 ttgagaatgc cgcaggcagc agggtgcgaa tagaatccca ggttagcgtg aattctgaat
    15301 taggcagatc ccacggcagc accagttgcg gcagcagttg cggaataccg ttaccctgag
    15361 aaccatcggc caggacgtag tggaattgcg aaccgatggt agcaacatgt ccgccgagca
    15421 ggttaacaat ccccatcacc gcgcaaccag ccagcaaggc cggaaggtga ccgggtaaac
    15481 gaatgcccag acgcggccag aaaaccagaa tgccaagcgt cacaatgcca atggcagcat
    15541 cacccacatt aatggtcggc agcgccataa ataatgcgcc gactttttgt agataatgtt
    15601 ccgggacatg ggccatttgc agaccgagaa aatctttaat ctgcatggta ccgatggtga
    15661 tcccgatacc cgaggtgaaa cctaaggtga cggaaaccgg aatatactca atcagacgac
    15721 caaagcgtgc cagccccatc agaatcaaaa agattcccga caacaaggtc gcaaccagca
    15781 gtcctgccag tccaaactgt tgcgaaacgg gatagagaat taccacaaat gccgcagtcg
    15841 gaccggaaac gctaaagcgt gacccacccg ttagagcaat gacaatcccc gcaacagctg
    15901 cggtatataa accgtactgg ggggccacac cactaccaat agccaacgcc atcgccagcg
    15961 ggatagcaat aatcccgacg gttatcccgg caatcaggtc acgggtaaac cgtgcggcag
    16021 tatatttttc tttccagcaa acgtcgatca gagcgcggaa aggcatcaca tgtgaggaaa
    16081 atattttgtt cacaataatg tttcatccgt gagcgcatca tctgtcaact aaatggcagg
    16141 tgaaggaggc ataggtcata caaatggata ttacagacaa aaaaacccgc cgcagcgggt
    16201 ctttgagccg ggttcgatta gtgttcgaac atggcagaga tcgattcttc gttgctgata
    16261 cgacgaatcg cttcggccag catacctgac agggtcagag tacgcacgtt cggcagtgat
    16321 ttgatttcat cgctcagcgg aatggtatcg cagacaacga cttcatcaat tacagagtta
    16381 cgcaggttgt tcgccgcgtt gccagagaag atcgggtgag tcgcgtacgc aaatacacgt
    16441 ttagcaccac gttctttcag cgcttcagca gctttacaca gcgtaccgcc agtgtcgatc
    16501 atatcatcga ccagtacgca gtcacgacct gcaacgtcac cgatgatatg catcacctgg
    16561 gaaacgttcg cacgcggacg acgtttgtcg atgattgcca tatcggtatc gttcagcagc
    16621 ttagcgatag cgcgggcacg cacaacgccg ccgatgtccg gagaaaccac aattgggtta
    16681 tccagattca gctgcagcat gtcttccagc aggatcgggc taccaaatac gttatcaacc
    16741 ggaacgtcga agaaaccctg aatctgttca gcgtgcagat ccactgtcag cacacggtca
    16801 acaccgacgc tggagaggaa gtctgcaacc actttcgcag tgattggtac acgagcggaa
    16861 cggacgcgac ggtcctggcg cgcatagcca aagtagggga taacagcggt gatacgacct
    16921 gcggaagcac gacgcagggc atcaaccata acgactaatt ccatcaggtt gtcgttagta
    16981 ggggcacaag tggactggat gatgaaaata tcaccaccgc gtacattttc gttaatttgt
    17041 acgctgactt cgccgtcgct aaagcgacct acagcggcgt cgccgagtga agtgtacagg
    17101 cggttggcaa tacgttgtgc tagtaccggg gtggcgttac cagcaaaaag cttcatatca
    17161 ggcacgagaa gaacctcagg catgcgtcca ttggtggaaa gaatctgccg aaaactgtgc
    17221 gggccaggca tgatcctttc caggcggtgt attaaagagc gcgatgcaac gtctggaaca
    17281 aggtgacgtt gtcaccgaaa ctcagcttgc ccggcttaaa gcatggctct gtgcaatggg
    17341 gaaagattaa cgcctttcgc cacaaagcca ttgagccatt ccggggcttg ctctagcacc
    17401 tggcgggctt cagactctgt atcaaattca gcaaagacac aggcccctgt cccagtcagg
    17461 cgtgacgggg cgtattctaa cagccaggaa agcaccgcat caacctcgcg aaaacgtttt
    17521 cttgcgataa cctcgcaatc attgctgaat tcacatttta gcaacgtttc tattgacctt
    17581 tttggcgtat tgcgcgggag ttcaggatct ttaaaaatca ccggagtcgg aatacttaca
    17641 ccagggtgcg ccaccagata ccacttctct ggcggatcca ccggcgttag tatttcacca
    17701 acgccttcgg caaacgcggc atgcccccga acaaagacag gaacatctgc gcccagcgtc
    17761 agccccattt ccgccagctc atccatgctt agcccgcatt gccagagatg atttaatgcc
    17821 accaggaccg tcgcggcatt ggatgaacca ccgccgagac cgccgcccat cggcaaacgc
    17881 ttgtcaatgc tgatattcgc accgcttccc gtcggaagac gcccgctgtc tgccgcagtt
    17941 ttcatcaaca accgcgctgc gcgaacgatc aggttatctt catgttccac gccttcaacg
    18001 ggcgttaaca gacgaatatc cccatcgtca cgaagctcaa tgctgatggt gtcgccgtaa
    18061 tcaagaaact gaaacagcgt ttgcagcgtg tggtaaccat ccgcacgctg accggtaatg
    18121 tataaaaaca gattaagttt tgccggagag ggccactgtg tccgcattat ttcactatcc
    18181 agttatccat ttttaacttg atgcgttgac caccgtcggt gagttccata ttggctggca
    18241 tcgcaggttg cgttttggtg tcataaccac cataaacaac cttccagttt ttgccattct
    18301 ggctgtaggt aatttcgctc aggcggtact ggtcgtccag tttgtagtcg gttgcatcac
    18361 ccggtaaacc taaaatccac tggcgcaagc tgttgagcgg aattggcatt ccggtcaatt
    18421 tgccaatcat ctcttcggcg tcatcggcgg tataacgctg acctttattg tcgactaact
    18481 gcacgttacc cggttgagca ttcagctcca gttccgtgct gcccaatggg ttagtgagca
    18541 gcagacggta gcgatcctgg ccggtttgct gccagaaaaa gcgggcgtac actttttgtt
    18601 ggtcagaaat ataagcgaac gcgccgcgag tctgatactg attaagattg cgcacgtctt
    18661 gctgatgctg acgccattgt ggcgaatccg ggcttttgcc aggacctttg ggcgtggtaa
    18721 cggaacaggc agtgagcaca agagcagcca gcggtagcag gcggataaga cgaaaatcgg
    18781 gcaggggcat agtgatgaca agtccttgag atacgttgca gttataaccc ttaatgctag
    18841 cgttaccgtc cgctatcgtc tatgttcaag ttgtcttaat tgccagaatc taacggcttt
    18901 cggcaattac tccaaaaggg ggcgctctct tttattgatc ttacgcatcc tgtatgatgc
    18961 aagcagacta accctatcaa cgttggtatt atttcccgca gacatgaccc ttttagcact
    19021 cggtatcaac cataaaacgg cacctgtatc gctgcgagaa cgtgtatcgt tttcgccgga
    19081 taagctcgat caggcgcttg acagcctgct tgcgcagccg atggtgcagg gcggcgtggt
    19141 gctgtcgacg tgcaaccgca cggaacttta tcttagcgtt gaagagcagg ataacctgca
    19201 agaggcgtta atccgctggc tttgcgatta tcacaatctt aatgaagaag atctgcgtaa
    19261 aagcctctac tggcatcagg ataacgacgc ggttagccat ttaatgcgtg ttgccagcgg
    19321 cctggattca ctggttcttg gggagccgca gatcctcggt caggttaaaa aagcgtttgt
    19381 cgattcgcaa aaaggtcata tgaaggccag cgaactggaa cgcatgttcc agaaatcttt
    19441 ctctgtagcg aaacgcgttc gcactgaaac agatatcggt gccagtgctg tgtctgtcgc
    19501 ttttgcggct tgtacgctgg cgcggcagat ctttgaatcg ctctctacgg tcacagtgtt
    19561 gctggtaggc gcgggcgaaa ccatcgagct ggtagcgcgt catctgcgcg aacataaagt
    19621 acagaagatg attatcgcca accgcactcg cgaacgtgcc caaattctgg cagatgaagt
    19681 tggcgcggaa gtgattgccc tgagtgagat cgacgaacgt ctgcgcgaag ccgatatcat
    19741 catcagttcc accgccagcc cgttaccgat tatcgggaaa ggcatggtgg agcgcgcatt
    19801 aaaaagtcgt cgtaaccaac caatgctgtt ggtggatatt gccgttccgc gcgatgttga
    19861 gccggaagtt ggcaaactgg cgaatgctta tctttatagt gtggacgatc tgcaaagcat
    19921 catttcgcac aacctggcgc agcgtaaagc cgcagcggtt gaggcggaaa ctattgtcgc
    19981 tcaggaaacc agcgaattta tggcgtggct gcgagcacaa agcgccagcg aaaccattcg
    20041 cgagtatcgc agccaggcag agcacgttcg cgatgagtta accgccaaag cgttagcggc
    20101 ccttgagcag ggcggcgacg cgcaagccat tatgcaggat ctggcatgga aactgactaa
    20161 ccgcttgatc catgcgccaa cgaaatcact tcaacaggcc gcccgtgacg gggataacga
    20221 acgcctgaat attctgcgcg acagcctcgg gctggagtag cagtacatca ttttcttttt
    20281 ttacagggtg catttacgcc tatgaagcct tctatcgttg ccaaactgga agccctgcat
    20341 gaacgccatg aagaagttca ggcgttgctg ggtgacgcgc aaactatcgc cgaccaggaa
    20401 cgttttcgcg cattatcacg cgaatatgcg cagttaagtg atgtttcgcg ctgttttacc
    20461 gactggcaac aggttcagga agatatcgaa accgcacaga tgatgctcga cgatcctgaa
    20521 atgcgtgaga tggcgcagga tgaactgcgc gaagctaaag aaaaaagcga gcaactggaa
    20581 cagcaattac aggttctatt actaccaaaa gatcctgatg acgaacgtaa cgccttcctc
    20641 gaagtccgtg ccggaaccgg cggcgacgaa gcagcgctgt ttgcaggcga tctgttccgt
    20701 atgtacagcc gttacgccga agcccgccgc tggcgggtag aaatcatgag cgccagcgag
    20761 ggtgaacatg gtggttataa agagatcatc gccaaaatta gcggtgatgg tgtgtatggt
    20821 cgtctgaaat ttgaatccgg cggtcatcgc gtgcaacgtg ttcctgctac ggaatcgcag
    20881 ggtcgtattc atacttctgc ttgtaccgtt gcggtaatgc cagaactgcc tgacgcagaa
    20941 ctgccggaca tcaacccagc agatttacgc attgatactt tccgctcgtc aggggcgggt
    21001 ggtcagcacg ttaacaccac cgattcggca attcgtatta ctcacttgcc gaccgggatt
    21061 gttgttgaat gtcaggacga acgttcacaa cataaaaaca aagcgaaggc gctttcagtg
    21121 ctcggtgctc gcatccacgc tgctgaaatg gcaaaacgcc aacaggccga agcgtctacc
    21181 cgtcgtaacc tgctggggag tggcgatcgc agcgaccgta accgtactta caatttcccg
    21241 caggggcgcg ttaccgatca ccgcatcaac ctgacgctct accgcctgga tgaagtgatg
    21301 gaaggtaagc tggatatgct gattgaaccg attatccagg aacatcaggc cgaccaactg
    21361 gcggcgttgt ccgagcagga ataatggaat atcaacactg gttacgtgaa gcaataagcc
    21421 aacttcaggc gagcgaaagc ccgcggcgtg atgctgaaat cctgctggag catgttaccg
    21481 gcagagggcg tacttttatt ctcgcctttg gtgaaacgca gctgactgac gaacaatgtc
    21541 agcaacttga tgcgctactg acacgtcgtc gcgatggtga acccattgct catttaaccg
    21601 gggtgcgaga attctggtcg ttgccgttat ttgtttcgcc agcgacctta attccgcgcc
    21661 cggatacgga gtgtctggtg gagcaggcac tggcgcggtt gcctgaacaa ccttgccgta
    21721 ttctcgatct cgggacgggt accggggcga ttgcgcttgc gctggctagc gagcgcccgg
    21781 actgcgaaat tatcgctgta gatcgtatgc ctgatgctgt ctccctggca caacgtaatg
    21841 cccagcatct ggcgatcaaa aatatccaca ttctgcaaag cgactggttt agcgcgctag
    21901 ccgggcagca gtttgcgatg attgtcagca atccgccgta tattgacgag caggacccac
    21961 atcttcaaca aggcgatgtc cgctttgagc cgctcactgc gctggttgcg gcagacagtg
    22021 gaatggcaga catcgtgcat atcatcgaac agtcgcgtaa cgcgctggta tccggcggct
    22081 ttctgcttct ggaacatggc tggcagcagg gcgaagcggt gcgacaagca tttatcctcg
    22141 cggggtatca tgacgtcgaa acctgccgtg actatggtga taacgagcgc gtaacgctcg
    22201 gccgctatta tcaatgacaa gtttttctac actgcttagt gttcatctta ttagtatcgc
    22261 gctttctgtt gggctattaa ccttacgttt ctggctacgt tatcagaagc atccacaggc
    22321 atttgctcgc tggacgcgca ttgtgccgcc ggttgtcgat acggtgttat tgttaagcgg
    22381 cattgcgttg atggctaaag cgcacatcct gccattttcc gggcaggcac agtggctgac
    22441 tgaaaagctg tttggagtta tcatttatat cgttttgggt tttattgcac tcgattatcg
    22501 tcgtatgcac agtcagcagg cgcgcattat tgccttcccg ctggcgttgg tggtgctgta
    22561 catcatcatt aaactcgcca ccacaaaagt accgttactg gggtaagtca tgagatcgtt
    22621 agctgatttc gaatttaata aagcgccatt gtgcgaaggc atgatcctgg cttgcgaagc
    22681 aatccgccgc gattttccct cgcaagatgt ttacgacgaa ctggagcgtc tcgttagtct
    22741 ggcgaaggaa gaaatcagcc agcttctgcc tttagaagag cagctggaaa aactgatcgc
    22801 gctgttttac ggcgactggg gatttaaagc ctcacgcggc gtttatcgtc tttccgatgc
    22861 attatggctg gaccaggtgt taaagaatcg acagggcagt gcggtatcat taggtgcggt
    22921 tttattatgg gtcgcgaatc gtctcgattt gccgctgctg ccggtgattt tccctacgca
    22981 gctgatattg cgcattgaat gtccggatgg cgaaatttgg ctgatt
//
LOCUS       DAZSNM010000046        22881 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZSNM010000046 DAZSNM010000000
VERSION     DAZSNM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22881
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.046"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            217..1443
                     /gene="mdfA"
                     /locus_tag="ACHW42_001509"
     CDS             217..1443
                     /gene="mdfA"
                     /locus_tag="ACHW42_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFR8943383.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(1484..1768)
                     /gene="ybjH"
                     /locus_tag="ACHW42_001510"
     CDS             complement(1484..1768)
                     /gene="ybjH"
                     /locus_tag="ACHW42_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR8943384.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(1854..2669)
                     /gene="ybjI"
                     /locus_tag="ACHW42_001511"
     CDS             complement(1854..2669)
                     /gene="ybjI"
                     /locus_tag="ACHW42_001511"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR8943385.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(2669..3877)
                     /locus_tag="ACHW42_001512"
     CDS             complement(2669..3877)
                     /locus_tag="ACHW42_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8943386.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            3961..4497
                     /gene="rcdA"
                     /locus_tag="ACHW42_001513"
     CDS             3961..4497
                     /gene="rcdA"
                     /locus_tag="ACHW42_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR8943387.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(4672..6357)
                     /locus_tag="ACHW42_001514"
     CDS             complement(4672..6357)
                     /locus_tag="ACHW42_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR8943388.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            6627..7004
                     /locus_tag="ACHW42_001515"
     CDS             6627..7004
                     /locus_tag="ACHW42_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR8943389.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(7034..7291)
                     /gene="grxA"
                     /locus_tag="ACHW42_001516"
     CDS             complement(7034..7291)
                     /gene="grxA"
                     /locus_tag="ACHW42_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR8943390.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            7451..7738
                     /locus_tag="ACHW42_001517"
     CDS             7451..7738
                     /locus_tag="ACHW42_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR8943391.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            7722..8444
                     /gene="nfsA"
                     /locus_tag="ACHW42_001518"
     CDS             7722..8444
                     /gene="nfsA"
                     /locus_tag="ACHW42_001518"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR8943392.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            8505..9407
                     /gene="rimK"
                     /locus_tag="ACHW42_001519"
     CDS             8505..9407
                     /gene="rimK"
                     /locus_tag="ACHW42_001519"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR8943393.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            9495..9971
                     /locus_tag="ACHW42_001520"
     CDS             9495..9971
                     /locus_tag="ACHW42_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR8943394.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            10323..11435
                     /gene="potF"
                     /locus_tag="ACHW42_001521"
     CDS             10323..11435
                     /gene="potF"
                     /locus_tag="ACHW42_001521"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR8943395.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVHENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            11530..12663
                     /gene="potG"
                     /locus_tag="ACHW42_001522"
     CDS             11530..12663
                     /gene="potG"
                     /locus_tag="ACHW42_001522"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR8943396.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            12673..13626
                     /gene="potH"
                     /locus_tag="ACHW42_001523"
     CDS             12673..13626
                     /gene="potH"
                     /locus_tag="ACHW42_001523"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR8943397.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            13623..14468
                     /gene="potI"
                     /locus_tag="ACHW42_001524"
     CDS             13623..14468
                     /gene="potI"
                     /locus_tag="ACHW42_001524"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR8943398.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            14528..15016
                     /locus_tag="ACHW42_001525"
     CDS             14528..15016
                     /locus_tag="ACHW42_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR8943399.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            15057..16184
                     /gene="rlmC"
                     /locus_tag="ACHW42_001526"
     CDS             15057..16184
                     /gene="rlmC"
                     /locus_tag="ACHW42_001526"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR8943400.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(16359..17090)
                     /gene="artJ"
                     /locus_tag="ACHW42_001527"
     CDS             complement(16359..17090)
                     /gene="artJ"
                     /locus_tag="ACHW42_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR8943401.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(17382..18050)
                     /gene="artM"
                     /locus_tag="ACHW42_001528"
     CDS             complement(17382..18050)
                     /gene="artM"
                     /locus_tag="ACHW42_001528"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR8943402.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(18050..18766)
                     /gene="artQ"
                     /locus_tag="ACHW42_001529"
     CDS             complement(18050..18766)
                     /gene="artQ"
                     /locus_tag="ACHW42_001529"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR8943403.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(18773..19504)
                     /gene="artI"
                     /locus_tag="ACHW42_001530"
     CDS             complement(18773..19504)
                     /gene="artI"
                     /locus_tag="ACHW42_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR8943404.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(19522..20250)
                     /gene="artP"
                     /locus_tag="ACHW42_001531"
     CDS             complement(19522..20250)
                     /gene="artP"
                     /locus_tag="ACHW42_001531"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR8943405.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(20468..20983)
                     /locus_tag="ACHW42_001532"
     CDS             complement(20468..20983)
                     /locus_tag="ACHW42_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR8943406.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            21632..>22881
                     /locus_tag="ACHW42_001533"
     CDS             21632..>22881
                     /locus_tag="ACHW42_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR8943407.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSV"
BASE COUNT         5709 a         5485 c         5826 g         5861 t
ORIGIN      
        1 aaactgggta aagcggcatc gtcttatttc cctcaagcgg cctgtttacg gtgggtgatt
       61 gtaacgggca taggttaaat aaaacttaaa gaaagcgtag ctatactcgc aataatgtaa
      121 gaatgtgctt aaccgtggtt tcagctacaa aattcgcttt ctcgttagct gcgcttttat
      181 taaactctgc gcgattatta ttggcgaaga aattgcatgc aaaataaatt agcttccggt
      241 gccaggcttg gacgtcaggc gttacttttc cctctctgtc tggtgcttta cgaattttca
      301 acctatatcg gcaacgatat gatccaaccc ggtatgttgg ccgtggtgga acaatatcag
      361 gcgggcattg attgggttcc tacttcgatg accgcgtatc tggcgggcgg gatgttttta
      421 caatggctcc tggggccgct gtcggatcgt attggtcgcc gtccggtgat gctggcgggt
      481 gtggtgtggt ttatcgtcac ctgtctggca atattactgg cgcaaaacat tgaacaattc
      541 accctgttgc gcttcttgca gggcataagc ttctgtttca ttggcgctgt gggatacgcc
      601 gcaattcagg aatccttcga agaggcggtt tgtatcaaga tcaccgcgct gatggcgaac
      661 gtggcgctga ttgctccgct acttggtccg ctggtgggcg cggcgtggat ccatgtgctg
      721 ccttgggagg ggatgtttgt tttgtttgcc gcattggcag cgatctcctt tttcggtctg
      781 caacgagcta tgcctgaaac cgccatgcgt ataggcgaga aactgtcact gaaagaactc
      841 ggtcgtgact ataagctggt gctgaagaac ggccgctttg tggcgggggc gctgggattc
      901 gttagcctgc cattgctggc gtggatcgcc cagtcgccga ttatcatcat taccggggag
      961 cagttgagca gctatgaata tggcttgctg caagtgccta ttttcggggc gttaattgcg
     1021 ggtaacttgc tgttagcgcg tctgacctcg cgccgcaccg tacgttcgct gattattatg
     1081 ggcggctggc cgattatgat tggtctgttg gtcgctgctg cggcaacggt tatctcatcg
     1141 cacgcgtatt tatggatgac cgccgggtta agtatttatg ctttcggtat tggtctggcg
     1201 aatgcgggac tggtgcgatt aaccctgttt gccagcgata tgagtaaagg tacggtttct
     1261 gcggcgatgg gaatgctgca aatgctgatc tttaccgtcg gtattgaaat cagcaaacat
     1321 gcctggctga acgggggcaa cggactgttt aatctcttca accttgtcaa cggaattttg
     1381 tggctgtcgc tgatggttat ctttttaaaa gataaacaga tgggaaattc tcacgaaggg
     1441 taaaaaaatg cctgattgat ttgtgcgatc aggcattctc gaattaatgg tgatggtcgt
     1501 caatctggtg ttcgataacc atcccttcac ctacgctggc aagatggcga acataaggat
     1561 gcgggcggta agccggagct ggcgcaggag ccacatagac ggtttgcggc acagtcgcga
     1621 cgctgaccgc ttgtggaacg ctgactgaat caggaaccac aaccactttg tatccactcg
     1681 gcacatcgac ggtgacactt tgcgccatcg ccacaccagt aaagcccatt aaaagcgcgc
     1741 ccaacagtag acaatttttc ataatcattt cgcctgaaat aatgcagcgg gttaatttcc
     1801 gcgaattatg cagccttcat tcaggcattg tgttgccggg cgtgctgccg tttttattgg
     1861 tcaaatggcg cttcgtgttt aagaacttta tcgatcacat ccagtacgcc ttcacggtta
     1921 ttgtagcctg cccggtattt tgccgctgcg acgactgcgc tgccggcatt ttccattgca
     1981 aaactaaagc ctgcctgacg cagcatctca atatcgttac cgccatcgcc aaagaccacc
     2041 acttcgctgt cgtctattcc ccataatttc tgcagttggc gaaggccatt ggctttatgt
     2101 acgccgggga taatcagatc gatgctgccg ttgccggtgt ggactggcac cataatatcg
     2161 ccgatggcct catgtaatgc tttttgtact tgtggaatca gttcatcgga aagattcagg
     2221 ccaaacttaa agaaaatatc ttctaagttg tcaaagttat cgacgtattc cagacggtga
     2281 taatacattt ccgccaccgt tttcatggca tcgtcatatt ttttgagtgt ataggcgcta
     2341 ttttttccgc aggcaataat ttccacttcc gggcgcgtca gcaaatgttc cacgacagta
     2401 gcaaacgcat ccttcgacag ctcgccatta aaaacatctt tgccttcact cattacccag
     2461 ccgccgtttt cagccacaaa ggcaatttca ttcgcaattt cagggaagaa agagatcaac
     2521 tgataatatt gattcccgct ggcgaccaca aagcgaatgc cttgtgcttt catttgctga
     2581 tactgagcca taaaccgctc acggttatag gttttttgat cgcttaagaa agtaccatcc
     2641 atgtctaccg caattaattt aatgctcatc aactattctc catcgccgtc tgcgttttgg
     2701 tatcgggttt ggcgacggct ttcgcgacaa tagccgcgag aataaccagc gccagtacaa
     2761 ccagcattgc actacgtaat ccataatgtt cgccgagata gcccagcagc ggcggcccga
     2821 cgaggaaagc cagataaccg gtcgttgcta ccacactgac gcgggttggt gcatcggggc
     2881 cggtatcgct ggcggcagaa atggtcagcg ggaagcccag cgaggcaccc agtccccaaa
     2941 gtacaacaga cactcctgcg acccaggcgc tatcgacaaa aataatcagc ccaataccca
     3001 acgcccccat tagtgcactg gcccgaacca cggcaacgcg actgtaactg tcgatgaacc
     3061 aaccgccggt gaagcgtcca acggtcatcc ccagggtaaa accggcataa atcagtgagc
     3121 cggaagtggg gctaaaaccg tgaccatcaa ccattaataa gggtaaccag tcgttggcag
     3181 aaccttcggc aaaggccatc gccagcacta caacaccaat caacagcaac tggatatcgc
     3241 gataaaaagg aatgcctttt tcgccatgct gggtgccatc ggcagcattt ttgcccgtac
     3301 cgtcagggat tgcctgaatg gcgatataaa taggcgcgat acctaccagc gccgccaata
     3361 aaatgtgcac cgttgccgga acgccaaagg ccgtcagtgc catcccgaca ccagcgcctg
     3421 ccagcgtgcc caggctataa aaaccgtgca tcatcggcaa aaccgtttta ttcatttctc
     3481 gctcaacggc ggcaccttca acgtttatcg ccacttccgc agaaccaaaa cttgccccaa
     3541 agacgccgag accaacggca aagagcaggg gcgatgtcag ccagagtgcc agacttaata
     3601 tcatcatccc gatcaatgcg caggacatcg tgaccaggat gacattacgt gtcccaaagc
     3661 gtttcactaa ccacgccgag cagagaatac cgctcatcga accgatcgac agaccaaaga
     3721 gaacaccgcc catttcagcg atcgagacag agagaatatc gcggatagca ggcgtacggg
     3781 ttgcccagga cgccattaac aggcctggca aaaagaagaa cataaacagc gcccaggttc
     3841 ggcgtttcaa tgcattacgt gaagaattta cggtcataga tcacgtcaaa ataagaagag
     3901 ggaagacaac attagcaagg ttgtgtacat ttgtacacaa ttgcagacag aggaaatgac
     3961 atgcgtcgcg ctaacgatcc gcaacggcga gaaaaaatta tccaggccac gctggaggcg
     4021 gtgaaacttt acggaataca tgctgttacg caccgcaaaa ttgctaccct tgccggggta
     4081 ccgttgggat cgatgaccta ctatttttca ggaattgatg agttgttgct ggaggcgttc
     4141 agcagtttta ctgagattat gtcccggcaa tatcaggcat tttttagcga tgttagtgat
     4201 gctcaaggcg catgccaggc tatcaccgat atgatctaca gctcacaggt tgcaacgccg
     4261 gataatatgg agctgatgta ccagctctac gcgctggcta gccgaaaacc gctattaaaa
     4321 acggtaatgc aaaactggat gcagcgcagt cagcaaacgc tcgaacaatg gtttgaaccc
     4381 ggaaccgccc gcgcgcttga tgcgtttatt gaggggatga cgctgcattt tgtcaccgac
     4441 cgtaagccgc tatcgcgcga ggagattttg aggatggttg agagggttgc agggtagtag
     4501 ataaatttta gataacaaaa aacccatcaa ccttgaaccg aaatggcggg gttgatgggc
     4561 tccacaaaat ggggacatca aagaaaagca gtggcactaa ttaagactga tgccctgcgg
     4621 aaaagttctg cggttgtgca aaaaaatttc attttcaggg caacttcagt tttatcctaa
     4681 tcctggccat accatgacga tgattgtccc tgccagcgtc agcaggacgt tggcgattgc
     4741 gtaggtgccc gcatagccca gcgcagggat gttactgcga gctgtatcac tgatgatctc
     4801 cattgccggc gcgcaggtgc gtgcgcccat cattgcgccg aacaacagtg cgcggttcat
     4861 tcgcaataca taagcaccga acaagaaaca gataaccacg ggcacaagac tgacaattaa
     4921 tccggcaatc aacatctgac cgccaatcgc gcccaggccg ttattaatac cgctaccggc
     4981 gctcagacca acgcctgcca taaacaccat caagccgaac tctttcacca tgcttaatgc
     5041 accttgcgga atgtaaccga aggtcgggtg gttagcacgc ataaagccca gcataattcc
     5101 ggcgaataac aacccggcag cgttccccat gccgaaactg aatgtgctga actggaaggt
     5161 gatcatcccg atcatcagcc caataacaaa gaaggcgcaa aatgccagca ggtcagtgac
     5221 ctggctgtga atcgagataa agccgatgcg atcggcgatg gtttttacgc gacgggcatc
     5281 accgctgact tgtaaaacgt cacctttgtt aagcacgacg ttgtcatcta tcggcatctc
     5341 aatctggcta cgaatgacgc ggttaaggaa gcaaccgtga tcggtcaact tcagttgtgc
     5401 gagacgttta cctacagcgt tatggttttt aacgaccact tcttcagtga cgatacgcat
     5461 gtcgagaagg tcacgatcga aaacttcttt accgttacgg aagctgggat cgagtcgggc
     5521 atgggcgtcg ggatagccta ccaacgctat ttcatcgccc atttgtagca cggcatcacc
     5581 gtctggattt gccagaatcc cgttacgtcg aatacgttca atgtagcagc cggtttgtcg
     5641 ataaataccc agttcacgca gatttttgcc gtcggtccag gccaccagct ccgggccgac
     5701 gcgataggcg cggatcaccg gtaaataaac cttacggttg gcatcagtgt ccaggccacg
     5761 ttcgcgggcg atttgctggg cgctggtctg taagtcctga tgctgcaatt tcggcaagta
     5821 acgcgcacca acaatcaaac tcaccagacc gattaaatag gttaaggcat acccgaggct
     5881 cagattatcc agtgccagtg agagctgcct gctttccatg ccggaatgat gcagtgtatc
     5941 gccagcaccg accagaaccg gtgtcgacgt catagagcct gctaacatac cggccgtcag
     6001 gccaatatcc cagccaaaca gcttacctaa ccctaaggcg atcaccagcg cactgccaac
     6061 catcaccagt gctaacatta ggtaattttt cccatcgcga aaaaaaatgg aaaaaaagtt
     6121 cggtccggct tcgaccccga cgcagaaaat aaacagcata aagccaagat taagcgcatc
     6181 ggtgttaatg ctgaaatgtt gttggcctaa taacagcgat acgactaaaa cgccaatgga
     6241 attacccagt tggatcgaac caagtcgtaa ctttccgaga catagcccaa gcgcgaggac
     6301 cacaaataat aacagaatgt aattcccatt taacaattcg gcgacgttta tattcacgga
     6361 ggctaacttc ttgtttacta gtaagctgtt gaaagaaatg ctaatttacg ataatgtgtt
     6421 ttaccagaat tcagggcgca gattcattca gcgcacctaa acgatagtaa agtaacaata
     6481 tattttacta gtgtaatcac attaggtatc aacggctata tgaattgcgt tggcctatat
     6541 tagcatggaa tgcgaagcgg ctttatctta ctgaacgcca cactggcgaa aaatgtgttc
     6601 gatagacgca gtgtcaggag gaacgagtga aacataaaca acgttgggcg ggggcaatct
     6661 gctgttttgt cctcttcatt gtggtgtgcc tttttctggc gacgcacatg aaaggcgctt
     6721 ttcgggctgc cgggcatcct gaaatcggct tgctgttttt cattcttcct ggagcagttg
     6781 ccagtttctt ttcacagcgt agagaagtcc tgaaacctct atttggcgca atgctggcgg
     6841 caccctgttc aatgctcatt atgcggctgt ttttttcacc gacgcgctca ttctggcaag
     6901 agctggcatg gttactaagc gcggtgttct ggtgtgcgct gggggcactg tgtttcttat
     6961 ttatcagtag tttgtttaaa ccacagcaca gaaaaaatca gtaaagccct caacgcgagg
     7021 gcttgtcaga cgatcaggcg tccagatttt ctttcaccca tgcagcaaaa tcggtatagc
     7081 cgccgatatg ttgctgatcg acaaaaatct gcggcacggt ttctacgggt ttacctgcct
     7141 tttgttgtag atcttcttta gtgatccctt ccgcacgaat atctacatac tgatactgaa
     7201 aatcatcgcg ttcattgctc aatttctcag ccagatcttt tgcacgcaca cagtaagggc
     7261 aacccgaacg accaaaaata acggtttgca ttatttctct cctcatagat ttatgcctgt
     7321 aatgatcacg ctaaaatgta ttcgctgaaa gtaggtttaa cctgttgcat taattgctaa
     7381 aagctataac tgttaaacac aatacagtga aaagttttag actgaaggct cactttgcag
     7441 agggaagcgt atgcgcgcga tcggtaaatt gcctaaaggc gtgttgatac tggaatttat
     7501 cggaatgatg ctactggcgg tggcgctgct gtcggtaagc gactccctgt cgctgcctga
     7561 gccattttct cggccagaag tgcagattct gatgattttt ctcggtgttt tgctcatgct
     7621 tcccgctgcg gtggtggtta ttcttcaggt ggcaaaacgt cttgccccac agctgatgaa
     7681 ctgtccaccg caatattcac gttcagaaag agaaaaagat aatgacgcca accattgaac
     7741 ttatttgtgg ccatcgcgcc attcgccatt tcactgatga acccatttcc gaagcgcagt
     7801 gtgaggcgat tattaacagc gcccgtgcga cgtccagttc cagttttttg cagtgtagta
     7861 gcattattcg cattaccgac aaagcattac gtgaagaact ggtgacgctg accggcgggc
     7921 aaaaacacgt agcgcaagcg gcggagttct gggtgttctg tgccgacttt aaccgccatt
     7981 tacagatctg tccggatgct cagctcggcc tggcggaaca actgttgctc ggtgtcgttg
     8041 atacggcaat gatggcgcag aatgcattaa ccgcagcgga atcgctggga ttgggcgggg
     8101 tatatatcgg cggcctgcgc aataatattg aagcggtgac gaaactgctg aaattaccgc
     8161 agcatgttct gccgctgttt gggctgtgcc ttggctggcc tgcggataat ccggatctca
     8221 agccgcgttt accggcctcc attttggtgc atgaaaacag ctatcaaccg ctggataaag
     8281 acgcactggc gcagtatgac gagcaactgg cggaatatta cctcacccgt ggcagcaata
     8341 atcgctggga tacctggagc gatcatatcc gccgaacaat cattaaagaa agccgcccat
     8401 ttattctgga ttatttgcac aaacagggtt gggcgacgcg ctaaaaccgc cacgtcgatg
     8461 tatgatacgc gggcttttga ccaggtctga cagagaggtg cagggtgaaa attgccatat
     8521 tgtcccggga tggaacgctc tattcgtgta agcggctgcg tgaagccgct atacagcgcg
     8581 gtcacctggt tgaaattctt gatccgcttt cttgctacat gaacataaat cctgcggcgt
     8641 cttctattta ctacaaaggc cgcaagttac cccattttga cgcagtgatc ccgcgtattg
     8701 gcaccgccat taccttttat gggacggcgg cactgcgcca gttcgagatg ctggggagct
     8761 atccgctcaa tgagtcggtc gccattgccc gggcgcgtga caaattgcgt tccatgcaac
     8821 tgctggcgcg tcagggcatc gacctgcctg tcacgggcat tgcgcattcg ccggatgata
     8881 ccagcgattt aatcgacatg gtcggtggtg cgccgctggt ggtcaagttg gttgaaggca
     8941 cgcagggaat tggcgtcgtg ctggcggaga ctcgtcaggc ggcggaaagc gtgattgacg
     9001 ctttccgcgg tctgaacgcg catattctgg tgcaggaata tatcaaagag gcgcaagggt
     9061 gcgatatccg ctgtctggtt gttggtgatg aagtggtcgc tgcgattgaa cggcgggcga
     9121 aagagggcta ttttcgttcc aatttgcatc gtggcggcgc ggcaagcgtc gccagtatca
     9181 caccacagga gcgtgaaatc gcgataaaag ccgcgcgaac gatggcgctg gacgttgctg
     9241 gtgtggatat tctgcgtgct aatcgcgggc cgttggtgat ggaagtgaat gcgtcgccgg
     9301 ggctggaagg aatagaaaaa accaccggta tcgatatcgc gggtaaaatg atccgctgga
     9361 tcgaacgcca cgctacgaca gaatattgcc tgaaaacggg tggttagtcg caatcacatt
     9421 actgatcatg gttttgcctg cgctttttgc gtaagctgtg ccggtctttt tatcgaaaga
     9481 ggttgtacaa aattatgaca tcgctggtcg ttcctggtct ggatacgctg cgtcaatggc
     9541 tcgatgacct ggggatgagt ttttttgaat gtgataactg tcaggctctg catctgcccc
     9601 atatgcagaa tttcgacggt gtctttgatg ccaaaatcga tctgatcgat aacacgatcc
     9661 tgttttctgc catggcggaa gtccgacctt cagccgtatt gccgctggcg gcggatttat
     9721 ctgccatcaa tgccagttcg ctgaccgtga aagcatttct tgatatgcag gatgataatc
     9781 tgccaaagct ggtggtttgc cagtctttat ccgttatgca gggcgtaacc tatgagcagt
     9841 ttgcatggtt cgtgcgtcag agcgaagagc agatttcgat ggtcattctt gaagctaatg
     9901 cccatcaact gctgttaccg actgacgatg aagggcaaaa caacgttacc gaaaactatt
     9961 ttctccactg ataattcctt tcgagcacgc agtcgctgcc gcagtgactg cgcgctgcaa
    10021 aattatctgc tgtttttaac cttttcttaa agattatttc acttctcttg tgtcgatttg
    10081 gctttatcac atagagcaaa tacgcataaa aatttgttaa ataccgtttt ttaatccgag
    10141 ctatagtctc aaaccctggc taaagttatt cttgcgatgc ttctatatag cgagcagtgc
    10201 tggccgggag agagttctct tttcttacac cgcgccgata aaaaaatatg cacgtttatt
    10261 gcatatcttt cagtgtgaca acttttgttc gtttgttaac gaactttcag aaggaaagag
    10321 atgtgaccgc cttaaataaa aaatggctat cgggtctggt tgcgggtgct ctgatggccg
    10381 tctctgtcgg cacgctcgcg gctgaacaaa aaacactcca catttataac tggtctgatt
    10441 atatcgcccc tgacacggtg gccaattttg aaaaagaaac cggtattaaa gtcgtctacg
    10501 atgttttcga ctctaacgaa gtactggaag gcaaattaat ggcgggtagc accggctttg
    10561 acttggtggt tccatctgcc agctttctag aacgccagtt gactgcggga gttttccagc
    10621 cgctggacaa aagcaaattg ccggagtgga agaatctcga cccggagctg ctgaagctgg
    10681 tcgccaaaca cgatcccgac aataaatttg ctatgcccta tatgtgggcg acgaccggga
    10741 ttggctataa cgttgataaa gttaaagctg tgctgggcga aaacgcgccc gtcgatagct
    10801 gggacttgat cctcaaacct gaaaatctgg aaaaactgaa aagctgcggt gtctctttcc
    10861 tggatgcgcc agaagaagtt tttgctaccg tgttgaatta tctcggcaaa gatcccaaca
    10921 gcactaaagc ggatgattac accggaccgg caaccgatct gctgttaaag ctgcgcccga
    10981 acattcgtta tttccattca tctcaataca ttaacgacct ggcaaacggc gatatttgcg
    11041 tcgctatcgg ctgggcaggt gatgtctggc aggcgtcaaa ccgcgcgaag gaagcgaaga
    11101 atggagtgaa tgtctcgttc tcgattccaa aagaaggggc gatggcgttc tttgatgtat
    11161 tcgccatgcc agcggatgcc aaaaacaaag acgaagccta tcagttcctg aattacctgc
    11221 tgcgcccgga tgtagtagcg catatttccg accatgtgtt ctatgtcaac gccaataaag
    11281 cagccacgcc gctggtgagt gcggaagtcc atgagaaccc gggtatttat ccgcctgcgg
    11341 atgttcgtgc gaagctgttc actctgaaag tgcaggaccc gaaaatcgac cgtgtgcgca
    11401 cccgcgcgtg gaccaaagtg aagagcggaa aataatccgc agccttagat gccgggcggg
    11461 cgcaccacac ccgccggcaa ttcgcaccat catggtgcgc ttgcacacat tcaatgccgg
    11521 agagcagccg tgaatgacgc tatccctcgc ccgcaggcga aaacccgtaa ggcgctgacg
    11581 ccgctattag aaatccgcaa cctcaccaaa tcctacgatg gtcaacatgc ggtggatgat
    11641 gtcagcctga ccatctacaa aggtgaaatc ttcgcgctgc tgggcgcatc cggctgtggc
    11701 aagtccacgc tgctgcgtat gctggcaggt ttcgaacaac cttctgccgg acagataatg
    11761 cttgatggcg tcgatttgtc acaggttccg ccttacctgc gccccatcaa tatgatgttt
    11821 cagtcttacg cgctgtttcc gcatatgacc gtggaacaga acatcgcttt tggcctgaaa
    11881 caggacaaac taccgaaagc ggaaatttcc agtcgggtca atgagatgct cggcctggtg
    11941 cacatgcagg agttcgccaa acgtaaaccg catcagcttt ctggtggtca gcgacagcgt
    12001 gtggccctgg cccgaagcct tgccaagcgc ccgaaactat tactgctcga cgagccgatg
    12061 ggcgcgctgg ataaaaagct gcgtgaccgg atgcagcttg aagtggtgga tattctggag
    12121 cgcgtcggtg tgacttgtgt gatggtcacc cacgatcagg aagaggcgat gaccatggcg
    12181 gggcgcatcg ccattatgaa tcgtgggaaa tttgtccaga ttggcgaacc ggaagagatc
    12241 tacgagcatc cgactacccg ctatagcgcc gaatttattg gctcggtaaa cgtctttgaa
    12301 ggcgtactca aagagcgtca ggaagatggc ttggtgcttg attcgccggg gctggtgcat
    12361 ccactgaaag tcgacgcgga tgcctcggtg gtcgataacg tgccggtaca tgtggcgctg
    12421 cgcccggaaa aaatcatgct ttgcgaagag ccgcccgcca atggttgtaa cttcgcggtg
    12481 ggggaggtga tacacattgc ctatctcggc gatctttcgg tgtatcacgt tcgtctgaaa
    12541 agtgggcaga tgattagtgc ccagctacaa aacgcccatc gtcatcgtaa agggttaccg
    12601 acctggggcg acgaagtgcg tttgtgctgg gaagtggaca gctgtgtggt gctgacggtt
    12661 taaggagcaa agatgaatac acttgaacct gctgcccagt cgaaaccgcc gggcggattt
    12721 aagctgtggt tgtcgcagct gcaaatgaag catgggcgca aactggtcat tgcgttgcca
    12781 tatatctggt tgatcttgct gtttctgctg ccatttctga ttgtctttaa aataagcctg
    12841 gcggagatgg cgcgcgctat tccaccttat accgaactga tggagtgggc tgacgggcaa
    12901 ctgtccatca ctcttaatct cggtaatttt ctgcaactga ccgacgatcc gctctatttc
    12961 gatgcttatc tccagtcgct acaggtggct gcgatttcga cattttgctg tttactgatc
    13021 ggctatccgc tggcgtgggc ggtggcgcac agtaagcctt cgacccgtaa tattttatta
    13081 ctactggtga tcctgccgtc gtggacctcg tttctgatcc gcgtttatgc ttggatggga
    13141 atattaaaaa acaacggtgt gctgaataat tttctgctgt ggctgggggt tatcgatcaa
    13201 ccgctgacca ttcttcatac caatctggca gtttatattg gcattgttta cgcctacgtg
    13261 ccgtttatgg tgctgccgat ttataccgcg ttgattcgta ttgattattc gctggtggaa
    13321 gcagcgctgg atctcggtgc acgaccgctg aaaacgttct ttactgtgat cgtgccgctg
    13381 actaaaggtg ggattattgc cggatcgatg ctggtgttta tcccggctgt gggcgagttt
    13441 gtgatcccgg aactgctcgg tggcccggac agcatcatga tcgggcgcgt gctatggcaa
    13501 gagtttttca ataaccgcga ctggccggtg gcctcggcgg tagcgatcat catgttgctg
    13561 ctgctaattg tgccgataat gtggttccac aaacaccagc aaaaaagcgt gggagaacac
    13621 ggatgaataa tttaccggta gttcgttcgc cctggcggat tgtgattttg ctgctgggct
    13681 ttacctttct ctacgcgcca atgctgatgc tggttatcta ttcgtttaac agctcgaagc
    13741 tggtgacggt gtggggcggc tggtcaacgc gctggtatgg tgagttattg cgcgatgacg
    13801 cgatgatgag tgcggttggt tcaagcctga caattgctgc ctgtgcggca acggcggcgg
    13861 cgatcctcgg gactattgcg gcggtggtgc tggtgcgctt tggcaggttt cgcggatcaa
    13921 atggctttgc ctttatgatc accgcgccgc tagtaatgcc agatgtcatc acgggcttgt
    13981 cgctgttgtt attattcgtc gcgcttgctc atgccattgg ctggcctgcg gaccgcggta
    14041 tgcttaccat ctggctggcg catgtcactt tctgtacggc ttatgtggcg gtcgttattt
    14101 cgtcgcgtct gcgggaactg gatcgctcga tagaagaagc cgcgatggat ctcggtgcga
    14161 cgccgctgaa agtgtttttc gtcattacgc taccgatgat catgcccgcg atcatttctg
    14221 gctggttact ggcttttact ttgtcgcttg atgatctggt gatcgccagc tttgtttccg
    14281 ggccgggagc caccacgtta ccgatgctgg tcttttccag cgtgcggatg ggggtgaatc
    14341 cggaaatcaa cgccctggca acattgatcc tcggcgcggt cggaattgtc ggatttatcg
    14401 cctggtatct gatggcgcgc gcagaaaaac agcggatacg cgacatccaa cgtgcaagac
    14461 gtggctgaag acactaaaat ttgccaacct ggctacataa tgccgcgcat gtcgcggcat
    14521 tgttttcatg gaagacgaaa cgttgggatt ttttaagaaa acatcttcat ctcatgctcg
    14581 cctgaatgtg cctgcgctgg tgcaggtggc ggcgctcgcc attattatga tccgtggcct
    14641 cgacgtgctg atgattttca atacgctggg cgtgcgcggt attggcgagt tcattcatcg
    14701 cagcgtacaa acctggagtt taacgctggt ctttttaagc agtctggtgc tggttttcat
    14761 tgagatctgg tgtgcgtttt cactggtgaa agggcgtcgc tgggcgcgct ggctatatct
    14821 gctgacacaa atcaccgccg caagttactt gtgggcggct tcgctggggt acggttatcc
    14881 ggagctgttc agcattcccg gtgaatcaaa acgtgaaatc ttccatagcc tgatgctgca
    14941 gaagctgccg gatatgctca tcctgatgct gctgttcgtt ccctcgacca gtcggcggtt
    15001 cttccagttg caataatgtg tataatcgtc gcccctgatg atgtgaaggt caatgtatgc
    15061 agtgcgcact ttacgacgcg ggtcgctgtc gttcctgtca gtggataacg cagccgattc
    15121 cagagcaact ctccgctaaa accgccgatc ttaaaaatct gctcgccgat tttccggttg
    15181 aggaatggtg cgcgccggtg tcaggcccgg aacaagggtt tcgtaataaa gccaaaatgg
    15241 tggtgagtgg tagcgttgaa aaaacactgc tcggtatgct gcatcgcgat ggcacgccgg
    15301 aagacctttg tgactgcccg ctttatccag cctcatttgc gcccgttttt gcggcgctaa
    15361 aaccgtttat cgcccgagcg gggttaacgc cctacaacgt ggcgcgtaag cgtggcgaac
    15421 tgaaatacat tctgctgact gaaagccaga gtgatggcgg catgatgctg cgctttgtac
    15481 tgcgttctga taccaagctg gcgcaactgc gtaaggcgct gccgtggtta cacgaacaac
    15541 taccgcagct gaaagttatt accgtcaata ttcagccggt acatatggcg attatggaag
    15601 gggagacgga gatctacctg accgaacaac aggcactggc ggagcgtttt aatgacgtac
    15661 cgctgtggat ccgtccgcaa agtttcttcc agactaatcc ggcggtcgcc agccagttgt
    15721 acgccaccgc gcgcgactgg gtacgacagc tgccggttaa acatatgtgg gatctgttct
    15781 gcggtgtggg gggctttggt ttacactgcg cgacgcctga catgcagtta accgggatcg
    15841 aaattgcgcc agaggccatt gcctgtgcaa agcagtcagc cgctgaactg ggcttaactc
    15901 gtttacaatt tcaggcgctg gactccactc agtttgccac tgctcagggg gaggtgccgg
    15961 agctggtgct ggttaacccg ccgcgccgcg gcattggtaa accgctgtgt gattatcttt
    16021 caacgatggc accgcgtttt attatctact ccagctgtaa cgcccaaacc atggcgaaag
    16081 atatccgcga actgccaggt taccgcatcg aacgggtaca gctttttgat atgttcccgc
    16141 acaccgcgca ctatgaagtg ttgacgctgc tggtgaagca ataaaaaagc cacaggtgcg
    16201 gcttcagatt gctgatcagg tgcgctttgt tcatgccgga tgcggcgtga acgccttatc
    16261 cggcctacaa aatcatgcta aatcaataca ttgcagagtt gatgtgggcc ggataagcgc
    16321 agcgcatcag gcaatcttac ttttgtcatc agtctcaatt actgcggaaa ccactggtca
    16381 ttgattttct gatatgtacc gtcagcttta attgctgcca gcgcgttatt cagtttttcc
    16441 agcagagctt tgttatccgg acgtacagcg atacccaggc cggtgccgaa gtattgcgga
    16501 tcggtcactt tttcagtcgc aacgcccagt tgcggattgg ttttcagcca ttcgtttacc
    16561 accgcggtgt caccaaatac cccatcaata cgaccatttt tcagatcgat aaaggcattc
    16621 tgataactgt cataggagac agttttcact tccgggtgct gatcctgaat atatttctgg
    16681 tgcgtcgtac cgttttccat cccaatgcgt ttgcctttca ggtcggcaaa cgttttgtag
    16741 gtatcttttt tggaaatcac gacggctgag ttttcatagt agggtgtggt aaacgaaacc
    16801 tgcttgctac gctccggggt gatatccata ccggagatta cggcgtcata ttttctgaat
    16861 ttcagtgacg ggatcaggct gtcgaacgcg tgattagtaa aagtacattc tgcctgcatt
    16921 tgtttgcaca aggcttttgc cagatcgata tcaaagccga caatctcatt attagcacct
    16981 atagattcaa agggtggata ggtggctgaa acgccaaaat tgattttctc tgcggcagaa
    17041 gcaccgaaag taaaggaagc aagtaaagcg gcaagaacta actttttcat gatggaactc
    17101 ccgtctgtca atcttatgat ttttggccgt gtctgcggca tgggataaca atgccatcaa
    17161 gtgaatttat atgcaataaa catgattaaa taatttaaat gaaataaaaa aagacggata
    17221 acttagtgag ttgtccgtct tcattataag aatttatgca ctatgtaggc cggataaggc
    17281 gtccacgccg catccggcac aggcaccgtg ctgatgtctg atgcgacgct ggcgcgtctt
    17341 atcagaccta caaacccccc cggcgaatgt acgcagctac attaattccg ccgttcgaat
    17401 gccagtgctt tgcgctcgat cagacgcatc atcagcgtca gcaggccgtt gacgaccaag
    17461 taaataatcc ctgccgcacc gaacaccatt acatcgtagg tgcgtccgta caacaactgg
    17521 ctgtatccca tcacttccat cagcgtaatg gtgtaagcca gagaggtact tttgaacacc
    17581 agcaccactt cgttggaata agaagagagc gagcgtttaa aggcatacgg cagcaggatc
    17641 gccagcgtat cttttttgct cattcccagg gcgctacagg actgccactg accttctggg
    17701 atcgcacgaa ttgcaccgta aaacagctgc gtggtatacg ccgcactgtt cagcgataac
    17761 gcaatcagcg cacataacca cggttctgac aacaaatgcc acagtgccgg atactcctgc
    17821 aacgtcggaa actggcccgg cccgtaataa atcaggaaga tctgcaccag cagcggcgta
    17881 ccggtaaaca gcgtgatata accccgcacc agccacacca gcaccggcgt tttcagcgtc
    17941 aggatgatgg taaaaatcaa tgccagaatc agtgccacaa tcagcgaggc aacggttagc
    18001 gtcaggctgg tgtgcagccc tttcatcagc tcgggtaaat actcaaacat tagctgggcc
    18061 tccgctcaaa acgtgtcgcg cgcaggtcaa tgcgtttgag aatgtactga ctgagcaggg
    18121 tgatcaccag gtaaatcgcc gccgccacaa tgtaccaggt aaatggttcc tgggtacgag
    18181 tagcaatgct tttggtttgt aacattaaat cattcacact aatcaaactg accagcgcgg
    18241 tatctttcag cagcaccagc cactggttac cgaggccagg cagcgcatga cgccacatct
    18301 gcggcatcac cagacggaaa aagatagccg atttcgacag ccccagcgcc tgaccagatt
    18361 cccactggcc caccggtaca gctttcaacg cgccgcgcag cgtttgcgag gcataggcgg
    18421 catacagcag tgacagagcg atgacaccac aaaggaacgg actcacgtcg aagttctcaa
    18481 tgtccatctg caccgggatc tgcacgaacc caagattgat agtgaagcca tccgaaagcg
    18541 tcagcagtaa ctgcgaggag ccaaaataga taaacagcac taccagaatt tctggcaggc
    18601 cacgcagaat ggttaccagc gctgaacctg cccacgcgac aggacgccat ttcgctgact
    18661 cccataccgc aaagaacatc gccagcgcca gcccgacaat caatgcacaa acggcaaggc
    18721 cgacggtcat cccggcggcg cttgctaaag gaaaaaattc attcatcagg aattacttct
    18781 ggaaccattt gttgtagatg gtttcgtaag tgccatcttt cttcactttt tccagcgcag
    18841 tgttgagttt ctgctgcagt tcagtgtttc cctgacgtac cgcgatgccg aggccagtac
    18901 cgaaataatc tttatcggtc actttatcgc ccactgccgc cagttttggg ttatctttca
    18961 gccactcagt gaccactgcg gtgtcaccaa atacgctgtc gatacgcccg ttttgcagat
    19021 ccagttttgc gttctggtag ctgtcatacg gaacggtggt gatttccggg tgcttatcca
    19081 taatgaactt ctggtgtgtc gtcccgttct gaacgccgac ttttttgcct ttcagctgat
    19141 caacactggt gtatttgcct tgctgaccca caaacagggc agagttgtca tagtacgggg
    19201 tagtaaacag tacctgcttt tcacgctccg gagtgatatc catgcccgcc atcactgctt
    19261 ctacgcgacg gaatttcagg cttgggatca ggctgtcaaa cgcctggtta gagaaagtac
    19321 aggttgcatc aatctcttta cacagtgcct gcgccaggtc gacgtcaaaa ccaacgatct
    19381 ggttgtttgc atcaatcgat tcaaacggag gatagggggc ttcggtagca aaacgaatgg
    19441 tttcggcagc tgtggcggaa agactaaaac ctgcaattaa cgcggcaatc agaacttttt
    19501 tcattgttgt tatcccgaat cttagtgaga gagatagttt ttaaatgctt cggtttgcgg
    19561 ctcggtaaag cagctcgcgt cgccttgttc tacgatatga ccattttcca tatacaccac
    19621 tcggctggcg gttttacgcg ccacttcaac ttcgtgggtg acgatcacct gggtaatatt
    19681 cgtttctgcc agctcacgaa tgatgctgac gatttgtgcc gtaatttccg ggtccagtgc
    19741 ggcggtcggt tcatcgaaca gcagtacctg cggttccatc atcaacgcac gggcaatagc
    19801 aacacgctgc tgctgaccac cagaaagatg cagcgggtaa cgatcgctat aaggtttgag
    19861 acgcagacgt tccagcagtt tttctgcacg ggccagcgcc tgatctttac tcaaccccag
    19921 tacacggcag ggcgcttcaa tcaggttttg ctgcacggtc agatgcggcc acaggttgta
    19981 ttgctgaaac accatgccaa cgttacgacg caaatcgcga atcgctttgt cagagggtgt
    20041 tttggtgaaa tcgaaatggt tgcctgcaat gttgagcgta ccggagcgcg gcatctcaag
    20101 cagattgagt acacgcagca gcgagctttt acccgcgccg ctggggccaa gtaacaccag
    20161 cgtttcgccc tgtgggcaat ccagcgtgat atcgaacagc gcctgatgcg cgccgtagaa
    20221 gcaattaatg ccgtttaatt gaatactcat tgacactcgt atactggcag tctgatagct
    20281 attgaggtcg aagatagtac ctttaacaga ataattatgc aatatttatg ctttaaaagt
    20341 taaaagcaaa gcgcattatt caataaacat agcacaaaat aacgggggcg gtggtcggtg
    20401 agcataaatg tcggcattcc tcacgaaatg ccggataatt tacggggttt attggttgat
    20461 caaggcgtta gcgattctcg atggactgac ggagcgtacc cgccgtggca tgaacgctac
    20521 cgcctaagta gcgcacatcg tcgatgaccc aacactggcc ttcctgaatc attaacactt
    20581 catcctgcca gccctggtca ccctgtttga gatccacccg caatggaatg ttacgggcat
    20641 cacgattagg gatagtcgat gcactggcaa cgtgggcgct gtctggcaag gtggttcgac
    20701 tggagaacgg atcgctgctt aacaattcac gatgactatt atcgcggctg gcatcgctaa
    20761 gttgggttgc cagtttgtca ctcaaatagg ggcgcagggc ggtaatgtca ttgctgcgat
    20821 gttgaatgcg gtagtcgtag aattgttgag ccacactgtc aggccctccc tggacacagg
    20881 aaccggtgcg ggggccgtta tctttataag ctggcgtgac ggtggtacag gcactcagca
    20941 gcagtgcaca tggaataagt agagatagtg aacggtagcg cataatcatt tccttattag
    21001 cttgctgtct gcgtcacact acgaccaaac gctatcagat tatttaatac attgttttta
    21061 atacaattct gaaacaatct gaacacctga ttgggtttga gtccattgct ggcgacccac
    21121 tcaaggttgc tcgtagctga cttacttaaa ttacccaaac aatagacagt gtaaaccatt
    21181 tgtgaggttg agcgtgttat atggctgaaa tgatcattta aggtaaatat gatagttctg
    21241 aaatggtggt ataaatcctc ctgtagaggt taagagcatc tgcaagaagc cagtttcaca
    21301 tttataacgc gtgataaatg aatgcggtta ttgtctaatc attacaaaat ttttcacatg
    21361 cgataagaga tcctgcgatt aagatgattg ccaaaattac gttgtatttt tgaaaatgag
    21421 cgaaatttga tagtcccagc tttgactctc gataatatta tttatgctac aattgtgggt
    21481 aaagaattta agctaatgaa tttgagtgaa gaataaatta aattgcttca gtatttcgtt
    21541 aattaatata aatacaaaag gcgttgtgct tttataatta taaagcgcga ggagttcggt
    21601 ggattatcgt ttttcttggg tgatttttaa tatgataaac aatatatctg atcaagcatc
    21661 atcatttcca ggcacacaac taaatcaaag tgataatttc ctggattcac tgagggaatt
    21721 tttcgcgata ttaaatccca gtcgtaaggg tgagttgtct acatgggata ccatctactt
    21781 gcatctgata ttagcaataa atgctgacag tgatctcata aaaaatgatg tgttgctcgc
    21841 ggaaaatatt ccatcagcga attaccaatt taatacattt tttagcaata ctttcgagat
    21901 tgatgttaaa aaatatctgg gtaaatcaga agataacgaa gttgaaataa aagcgggtaa
    21961 tgaaagaatc tctattggaa tacgtaatgt atcgaatgga aaacttgaaa gacaacagtt
    22021 tcttttccca ctggactatg agaataaatt acaagaacaa ttagataagt attttacgat
    22081 tgaatcgcac ccattattat acaggtatac cattggtagt aagattgcga atgtgatttt
    22141 tgaaaagtta tacagtcgta ttgactttaa caaagaacaa tatatatcct ttataaagga
    22201 cgcatttatt catttttatg attatagcag gcgatatgct attagtgaga atatcgataa
    22261 agacgctgta acaaacaata tcgcattgat gagcactttt tatgacagtg ataatacgtc
    22321 aggtgaggtt ttaaataatg attttacaga agaagagtca tttgaaactg cgttagatgt
    22381 agagcatgca atagtgcttg gatttgctga tgataatttt gaaacaaaac ctgttcatta
    22441 tcaggattta ttaacacgat ttagtgcctt tcaagatacc gtgtttaatc tgtttcctga
    22501 aatgcactct tcgcattacc acgatatttg tagcgtaagt gtggatatga caaagggaac
    22561 gcaatgcatg attcacttga tggtgaatga agaagttttc atgtctctgc ctgttccagt
    22621 tgccacgatg gtacgtgaag atgcaagtaa tttagtcaat cttaaaacac tactcaatga
    22681 tggttgtttc attaaatata gtcattttaa tgacgtggca ttaataaagc aaaacataag
    22741 taatctctat ttatcacaca ctgttgtgaa tgaaagcata ttgaaaaaat gttgttttga
    22801 aaacggcagt ttaggtgatg ttaaaataac aaatagcaat gtaataaaca gtgcattcaa
    22861 aaatatttcg tttagatctg t
//
LOCUS       DAZSNM010000047        22690 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZSNM010000047 DAZSNM010000000
VERSION     DAZSNM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22690
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.047"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1485
                     /locus_tag="ACHW42_001534"
     CDS             <1..1485
                     /locus_tag="ACHW42_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR8943408.1"
                     /translation="MPQKFGLYEDLTVMENLNLYADLRSVTGEARKQTFARLLEFTSL
                     GPFTGRLAGKLSGGMKQKLGLACTLVGEPKVLLLDEPGVGVDPISRRELWQMVHELAG
                     EGMLILWSTSYLDEAEQCRDVLLMNEGELLYQGEPKALTQTMAGRSFLMTSPHEGNRK
                     LLQRALKLPQVSDGMIQGKSVRLILKKEATPDDIRHADGMPEININETTPRFEDAFID
                     LLGGAGTSESPLGAILHTVEGTPGETVIEAKELTKKFGDFAATDHVNFAVKRGEIFGL
                     LGPNGAGKSTTFKMMCGLLVPTSGQALVLGMDLKESSGKARQHLGYMAQKFSLYGNLT
                     VEQNLRFFSGVYGLRGRAQNEKISRMSEAFGLKSIASHATDELPLGFKQRLALACSLM
                     HEPDILFLDEPTSGVDPLTRREFWLHINSMVEKGVTVMVTTHFMDEAEYCDRIGLVYR
                     GKLIASGTPDDLKAQSANDEQPDPTMEQAFIQLIHDWDKEHSNE"
     gene            1478..2611
                     /locus_tag="ACHW42_001535"
     CDS             1478..2611
                     /locus_tag="ACHW42_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8943409.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            2622..3728
                     /locus_tag="ACHW42_001536"
     CDS             2622..3728
                     /locus_tag="ACHW42_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8943410.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(3690..4100)
                     /locus_tag="ACHW42_001537"
     CDS             complement(3690..4100)
                     /locus_tag="ACHW42_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR8943411.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            4233..4994
                     /locus_tag="ACHW42_001538"
     CDS             4233..4994
                     /locus_tag="ACHW42_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR8943412.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            4991..6232
                     /gene="clsB"
                     /locus_tag="ACHW42_001539"
     CDS             4991..6232
                     /gene="clsB"
                     /locus_tag="ACHW42_001539"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR8943413.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            6232..7188
                     /locus_tag="ACHW42_001540"
     CDS             6232..7188
                     /locus_tag="ACHW42_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFR8943414.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(7224..7937)
                     /locus_tag="ACHW42_001541"
     CDS             complement(7224..7937)
                     /locus_tag="ACHW42_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR8943415.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(8142..8846)
                     /locus_tag="ACHW42_001542"
     CDS             complement(8142..8846)
                     /locus_tag="ACHW42_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR8943416.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(8983..9435)
                     /gene="moaE"
                     /locus_tag="ACHW42_001543"
     CDS             complement(8983..9435)
                     /gene="moaE"
                     /locus_tag="ACHW42_001543"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR8943417.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(9437..9682)
                     /gene="moaD"
                     /locus_tag="ACHW42_001544"
     CDS             complement(9437..9682)
                     /gene="moaD"
                     /locus_tag="ACHW42_001544"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR8943418.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(9675..10160)
                     /gene="moaC"
                     /locus_tag="ACHW42_001545"
     CDS             complement(9675..10160)
                     /gene="moaC"
                     /locus_tag="ACHW42_001545"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR8943419.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(10163..10675)
                     /gene="moaB"
                     /locus_tag="ACHW42_001546"
     CDS             complement(10163..10675)
                     /gene="moaB"
                     /locus_tag="ACHW42_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR8943420.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(10697..11686)
                     /gene="moaA"
                     /locus_tag="ACHW42_001547"
     CDS             complement(10697..11686)
                     /gene="moaA"
                     /locus_tag="ACHW42_001547"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR8943421.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(11674..11818)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            12083..12991
                     /gene="yvcK"
                     /locus_tag="ACHW42_001548"
     CDS             12083..12991
                     /gene="yvcK"
                     /locus_tag="ACHW42_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR8943422.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(13183..15204)
                     /gene="uvrB"
                     /locus_tag="ACHW42_001549"
     CDS             complement(13183..15204)
                     /gene="uvrB"
                     /locus_tag="ACHW42_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR8943423.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPEALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(15783..16460)
                     /gene="bioD"
                     /locus_tag="ACHW42_001550"
     CDS             complement(15783..16460)
                     /gene="bioD"
                     /locus_tag="ACHW42_001550"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR8943424.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(16453..17208)
                     /gene="bioC"
                     /locus_tag="ACHW42_001551"
     CDS             complement(16453..17208)
                     /gene="bioC"
                     /locus_tag="ACHW42_001551"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR8943425.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(17195..18349)
                     /gene="bioF"
                     /locus_tag="ACHW42_001552"
     CDS             complement(17195..18349)
                     /gene="bioF"
                     /locus_tag="ACHW42_001552"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR8943426.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(18346..19386)
                     /gene="bioB"
                     /locus_tag="ACHW42_001553"
     CDS             complement(18346..19386)
                     /gene="bioB"
                     /locus_tag="ACHW42_001553"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR8943427.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            19473..20762
                     /gene="bioA"
                     /locus_tag="ACHW42_001554"
     CDS             19473..20762
                     /gene="bioA"
                     /locus_tag="ACHW42_001554"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR8943428.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            20821..21297
                     /locus_tag="ACHW42_001555"
     CDS             20821..21297
                     /locus_tag="ACHW42_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR8943429.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            21612..21692
                     /locus_tag="ACHW42_001556"
                     /pseudo
     CDS             21612..21692
                     /locus_tag="ACHW42_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21702..21788)
                     /locus_tag="ACHW42_001557"
                     /pseudo
     CDS             complement(21702..21788)
                     /locus_tag="ACHW42_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            21880..>22690
                     /locus_tag="ACHW42_001558"
     CDS             21880..>22690
                     /locus_tag="ACHW42_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8943430.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLT"
BASE COUNT         5335 a         6086 c         5781 g         5488 t
ORIGIN      
        1 atgccgcaga aatttggtct gtatgaagat ctcacggtga tggagaacct caatctgtac
       61 gcggatttgc gcagcgtcac cggcgaggca cgtaagcaaa cttttgctcg cctgctggag
      121 tttacgtctc ttgggccgtt taccggacgc ctggcgggca agctctccgg tgggatgaaa
      181 caaaaactcg gcctggcctg taccctggtg ggcgaaccga aagtgttgct gctcgatgaa
      241 cccggcgtcg gcgttgaccc tatctcacgg cgcgaactgt ggcagatggt gcatgagctg
      301 gcgggcgaag ggatgttaat cctctggagt acctcgtatc tcgacgaagc cgagcagtgc
      361 cgtgacgtgt tactgatgaa cgaaggcgag ttgctgtatc agggagaacc aaaagccctg
      421 acacaaacca tggccggacg cagctttctg atgaccagtc cacacgaggg caaccgcaaa
      481 ctgttgcaac gcgccttgaa actgccgcag gtcagcgatg gcatgattca ggggaaatcg
      541 gtacgtctga tcctcaaaaa agaggccaca ccagacgata ttcgccatgc cgacgggatg
      601 ccggaaatca acatcaacga aactacgccg cgttttgaag atgcgtttat tgatttgctg
      661 ggcggtgccg gaacctcaga atcgccgctg ggtgcaatat tgcatacggt agaaggcaca
      721 cccggcgaga cggtgatcga agcgaaagaa ctgaccaaga aatttggtga ttttgccgcc
      781 accgatcacg tcaactttgc cgttaaacgt ggggagattt ttggtttgct ggggccaaac
      841 ggcgcgggta aatcgaccac ctttaagatg atgtgcggtt tgctggtgcc gacttccggc
      901 caggcgctgg tgctggggat ggatctgaaa gagagttccg gtaaagcgcg ccagcatctc
      961 ggctatatgg cgcaaaaatt ttcgctctac ggcaacctga cggtcgaaca gaatttacgc
     1021 tttttctctg gtgtgtatgg cttacgcggt cgggcgcaga acgaaaaaat ctcccgtatg
     1081 agcgaggcat ttggcctgaa aagtattgcc tcacacgcca ccgatgaact gccattaggt
     1141 tttaaacagc ggctggcgct ggcctgttcg ctgatgcatg aaccggatat tctctttctc
     1201 gacgaaccga cttccggcgt tgaccccctc acccgccgtg aattttggct gcacatcaac
     1261 agcatggtag agaaaggcgt cacggtgatg gtcaccaccc actttatgga tgaagcggaa
     1321 tattgcgacc gcatcggcct ggtgtaccgt gggaaattaa tcgccagcgg tacgccggac
     1381 gatttgaaag cacagtcagc taacgatgag caacccgatc ccaccatgga gcaagccttt
     1441 attcagttga tccacgactg ggataaggag catagcaatg agtaacccga tcctttcctg
     1501 gcatcgcgtg cgggcgctgt gcgtcaaaga gacgcggcag atcgttcgcg atccgagtag
     1561 ctggctgatt gcggtagtga tcccactgct actgctgttt atttttggtt acggcattaa
     1621 cctcgactcc agcaagctgc gggtcgggat tttgctggaa cagcgtagcg aagcggcgct
     1681 ggatttcacc cacaccatga ccggttcgcc ctacatcgac gccaccatca gcgataaccg
     1741 tcaggaactg atcgccaaaa tgcagtcggg gaaaattcgc ggtctggtgg ttattcccgt
     1801 ggatttcgcc gaacagatgg agcgcgccaa cgccaccgca ccgattcagg tgatcaccga
     1861 cggcagtgag ccgaataccg ctaactttgt gcaggggtat gtcgaaggga tctggcagat
     1921 ctggcaaatg cagcgagcgg aggacaacgg gcagactttt gaaccgctta ttgatgtaca
     1981 aacccgctac tggtttaacc cggcggcgat aagccagcac tttattatcc cgggtgcggt
     2041 gaccattatc atgacggtca tcggcgcgat tctcacctcg ctggtggtgg cgcgagaatg
     2101 ggaacgcggc accatggagg ctctgctctc tacggagatt acccgcacgg aactgctgct
     2161 gtgtaagctg atcccttatt actttctcgg gatgctggcg atgttgctgt gtatgctggt
     2221 gtcagtgttt attctcggcg tgccgtatcg cggttcgctg ctgattctgt tttttatctc
     2281 cagcctgttt ttactcagta ccctgggaat ggggctgctg atttccacga ttacccgcaa
     2341 ccagttcaat gccgctcagg tcgccctgaa cgccgctttt ctgccgtcga ttatgctttc
     2401 cggctttatt tttcagatcg acagtatgcc cgcggtgatc cgcgcggtga cgtacattat
     2461 tcccgctcgt tatttcgtca gcaccctgca aagcctgttc ctcgccggga atattccagt
     2521 ggtgctggtg gtaaacgtgc tgtttttgat cgcttcggcg gtgatgttta tcggcctgac
     2581 gtggctgaaa accaaacgtc ggctggatta gggagaagag catgtttcat cgcttatgga
     2641 cgttaatccg caaagagttg cagtcgttgc tgcgcgaacc acaaacccgc gcgattctga
     2701 ttttacccgt gctaattcag gtgatcctgt tcccgttcgc cgccacgctg gaagtgacta
     2761 acgccaccat cgccatctac gatgaagata acggcgagca ttcggtggaa cttactcaac
     2821 gttttgcccg cgccagcgcc tttacacatg tgctgctgct gaaaagccca caggagatcc
     2881 gcccgaccat cgacacacaa aaggcgttac tactggtgcg tttcccggct gacttctcgc
     2941 gcaaactgga taccttccag accgcgccgt tgcagttgat cctcgacggg cgtaactcca
     3001 acagtgcgca aattgccgcc aactacctgc aacagatcgt caaaaattat cagcaggagc
     3061 tgctggaagg aaaaccgaaa cccaacaaca gcgagctggt ggtacgcaac tggtataacc
     3121 cgaatctcga ctacaaatgg tttgtggtgc cgtcgctgat cgccatgatc accactatcg
     3181 gcgtaatgat cgtcacttca ctttccgtcg cccgcgaacg tgaacaaggt acgctcgatc
     3241 agctactggt ttcgccgctc accacctggc agatcttcat cggcaaagcc gtaccggcgt
     3301 taattgtcgc caccttccag gccaccattg tgctggcgat tggtatctgg gcgtatcaaa
     3361 tccccttcgc cggatcgcta gcgctgttct actttacgat ggtgatttac ggtttatcgc
     3421 tggtgggatt cggtctgttg atttcatcac tctgctcgac acaacagcag gcgtttatcg
     3481 gcgtgtttgt ttttatgatg cccgccattc tcctttccgg ttacgtttcg ccggtggaaa
     3541 acatgccagt gtggctgcaa aatctgacgt ggattaaccc tattcgccac tttacggaca
     3601 ttaccaagca gatttatttg aaggatgcga gtctggatat tgtgtggaat agtttgtggc
     3661 cgctactggt gataacggcc acgacagggt cagcggcgta cgcgatgttt agacgtaagg
     3721 tgatgtaact tcttatcttt cgccagcaaa gacactaccg ccgggccagc aaggatcgcc
     3781 agccctgcaa ttgccagcaa aaagttgttt tccagtacgc gggacaacag ccacagcaca
     3841 atcagcgctg cgccaaaata ccaggtcgtg gtcagctctt ccagcacgtc tgccacctca
     3901 cgccagcgtt gctcggggtg gcgctgaaac accagcatca tcactaccgt gacacaatac
     3961 agtgcgcata atcccacgcc gacgtgaacc agagtcttac tcatccagcc caccaccagc
     4021 agcgccacta ccagtaaatg caacataatc tgccagcaac ttagacccgt tgcgacacga
     4081 acacgttgtt gccacttcat ggcttctctc ctgaagaatt tatctctact tactcagagt
     4141 accgaacttt acggaaaatt ccgtaagacc gcaccttttt gtgacgccga ctacactatt
     4201 tcaatgagat aagaggcaaa gacaggaaaa ttatgcccga tcaaacacaa caattttcgt
     4261 tcaaggtgct caccatcaac attcacaaag gctttaccgc gtttaaccga cgcttcattt
     4321 tgccggaact tcgcgatgcc gtgcgtaccg tcagcgccga tattgtttgc ctgcaggaag
     4381 tgatgggcgc gcacgaagtt catccgctgc atgtggaaaa ctggcccgat acctcgcact
     4441 acgagtttct cgccgacact atgtggagcg attttgccta cggtcgcaat gccgtatacc
     4501 cggaagggca tcacggcaac gccgtactgt cgcgttatcc cattgaacat tatgagaatc
     4561 gcgatgtttc ggtcgatggt gcggaaaagc gcggcgtgct ctactgccgc attgtgccgc
     4621 cgatgaccgg aaaagcgatt catgtgatgt gcgtacatct gggcctgcgt gaggcgcacc
     4681 gtcaggcgca gcttgcgatg ctcgccgaat gggtgaatga gctaccggac ggcgaaccgg
     4741 tattggtggc gggtgatttc aatgactggc ggcaaaaagc taatcatccg ttaaaagtgc
     4801 aggccggact ggatgagatt tttacccgcg cccacggacg cccggcgcgc acgtttccgg
     4861 tgcaatttcc tctactacga ctggacagga tctacgtcaa aaatgccagc gccagcgcgc
     4921 caaccgcgtt gccgctgcgg acatggcgac acctttctga tcatgcccct ttaagtgcgg
     4981 agattcattt atgaaatgta gctggcgcga aggcaataag atccagttgc tggaaaacgg
     5041 cgagcaatat tatcccgcgg tgtttaaggc gattggcgag gcacaagaac gcatcattct
     5101 tgaaacgttt atctggtttg aggataacgt cggcaaacag ctgcatgcgg cactactggc
     5161 agcagcgcaa cgcggggtta aagcggaagt cttgctggat ggctacggtt cgccggatct
     5221 cagcgatgag tttgtcaatg aactgacggc agctggcgta gtgttccgct actacgatcc
     5281 ccgccctcgc ctttttggta tgcgcaccaa tgtgtttcgc cggatgcatc gcaaaattgt
     5341 ggtgatcgac gcgcgtatcg cctttattgg cgggctgaat tactccgccg agcatatgtc
     5401 cagctacggt ccagaggcta aacaggatta cgcggtacgc cttgaagggc cgattgtcga
     5461 agatattctc cagtttgagc tggaaaacct gcctggacag agcgcggcac gacgctggtg
     5521 gcgacgtcat cacaaagcgg aagagaaccg ccagccggga gaagcgcagg tattgctggt
     5581 ctggcgcgat aacgaagaac atcgcgatga tattgaacgc cattatctga aaatgctcac
     5641 tcaggcgcag cgggaagtga ttatcgccaa cgcctacttc ttccccggct atcgattttt
     5701 acacgccttg cgtaaagcgg cacggcgcgg ggtgcggatc aaactgatca ttcagggcga
     5761 accggatatg ccgattgtca gagtcggtgc gcgcttgctg tataactatc tggttaaagg
     5821 cggcgttcag gtttttgagt accgccgccg cccgctccac ggcaaagtgg cattgatgga
     5881 cgatcactgg gcgacagtag ggtccagtaa tctcgatccg ctcagtttgt cactgaatct
     5941 cgaagcaaat gtcatcatcc acgatcgtca ttttaaccag acgctgcgcg ataatctgaa
     6001 cggcattatt gccgcagatt gtcagcaggt ggatgaaacc atgctgccca aacgcacctg
     6061 gtggaacctg accaaaagcg tgctggcgtt ccacttttta cgccacttcc cggcgctggt
     6121 tggctggctt ccggcacaca caccacgtct gacgcaggtt gatccccccg cacaaccgac
     6181 aatggaaacg caggatcggg tagaaactga aaacacgggg gtaaacccct gatgagtaaa
     6241 tcacacccgc gctggcgctt agcaaagaag atcctcacct ggctgttttt tatcgcggtg
     6301 attgtgttgc tggtggtcta cgccaaaaaa gtggactggg aagaggtctg gaaggtcatc
     6361 cgcgactaca atcgcgttgc gctgcttagt gcggtcgggc tggtggtcgt cagctatctg
     6421 atttacggct gctatgacct gctcgcccgc ttttactgcg gtcacaaact ggcgaagcgc
     6481 caggtgatgc tggtgtcgtt tatctgctac gccttcaacc tgacgctcag tacctgggtc
     6541 ggcggcattg gtatgcgcta tcgtttgtac tcccgactag ggttaccggg cagcactatt
     6601 acgcggattt cctcgctcag tattaccacc aactggctgg gctatatttt actggcaggg
     6661 attatcttta ccgcaggcgt ggtggagttg ccggattact ggtatgtcga tcaaactacg
     6721 ctgcgtattc tcggcattgg cttactgatg attatcgcgg tttatttgtg gttttgcgct
     6781 ttcgcgaagc accgccatat gaccatcaaa gggcaaaagc tggtgctgcc ttcatggaaa
     6841 ttcgccctcg ctcagatgct gatttccagc gttaactgga tggtaatggg ggcgattatc
     6901 tggctgttac ttggtcaaag cgtgaactat ttctttgtac tgggcgtgtt actggttagt
     6961 agtattgctg gcgtcatcgt gcatattccg gcggggatcg gtgtgctgga agcggtgttt
     7021 atcgcactac tggctgggga gcatacctcg aaagggagca ttatcgccgc cctgctcgct
     7081 tatcgcgtcc tgtattactt tatcccgctg ctgctggcgc tgatctgtta cctgctactg
     7141 gaaagccaag cgaagaagct acgggcgaaa aatgaagcgg cgatgtgaag tttagttaag
     7201 ttctttagta tctgcattta cgactaatga aaaaaacgcg tatgcctttg ccagacaagc
     7261 gttatagcta tcgcggcaag tatcccaaag aagccgataa aatagtaata gagtgttaat
     7321 gcacctgtaa aagcgcaacg aaccggactc cacaactctt cactatggca ttttcctgcc
     7381 agttcaagga gcttatttcg tccatgcgag ataatgcctg gccataacac aatcattaag
     7441 caacttatta cccaaatggg ccgttcactc attaatgccg cattcacgat gattaccagg
     7501 gccaaccctg tcaacgtcat cataatgata aaacggtgag aaccaggatc aacattaaat
     7561 aataaaccgg ccagcatact gattaaaaac atcgcgcccg cagtgctgaa cacgatgata
     7621 atggcatata caggaactaa acttgcaacg acaatcgcca ccaaagagag taaaaacaga
     7681 tatattacgc ccgcgatggc ggcatttatc ttgttgatat ccttacttaa gccatacata
     7741 caaagtaata ataaccctat ggcgacatac attggtggtg aatcatattt ggttgccgta
     7801 atttcgagat acttagctcc ccaggctagt cctgcggttg ccaccaaacc taaagcggta
     7861 atgagccata taccgtttaa tatcctggcc tcgtgctgaa aatctaattt attgctgttt
     7921 tgcgagtatg attccattct tttagttgtc cttagaaaaa acattaatat tcaccatcaa
     7981 taaatttaaa accacgcata caaaatactt ttgtatttat ggatacggaa aacgattccg
     8041 ctcgctcata aggcatattt tatacctggt acttgttaag tgtcgttttt agtaagaaaa
     8101 aaatcccgac acatggccgg gatagtgatg gtgaaaaaga attaacggcg gttgccgaag
     8161 atccgtaaca acatcaggaa caggttgatg aagtccagat acaaggttaa cgcaccaaga
     8221 atggaatatt tgcgcaggtt cgacgtgtcg cgggtatcaa tctgctcacc catatttttc
     8281 aatttctgag tgtcatacgc cgtcaatccg acaaagacaa tcacgccgat gtaggtaact
     8341 gcccacatca atgcttcgct tttcagccag aagttgacca gcgatgccag cacaatgccg
     8401 attaacgcca taaacagcat attgccaaag ccacttaaat cgcgcttcgt ggtgtaaccg
     8461 tacaggctca ttgcgccgaa catcccggca gtaacgacga aagtactggc gatagaagca
     8521 gcggtataga caatgaatat actggaaagc gtaagacccg tcagcgccga ataaagcata
     8581 aagagcatcg tcgttacacc tgcgctcagc ttttgaatca tcgctgataa cacaataacc
     8641 aatgctaatt gcgcgatgat cagaccgatt aaaaagacac ggttagtgaa caacagctcc
     8701 atcacggccg cggaattagc cgcataccag gcaacaaatg cggtcagcaa caagccaacg
     8761 gtcatccagc catagacttg cgccatataa gtttgcaagc cagcccaggg ttgtacgatt
     8821 gaatcagaac gtgggaatct gtccatgacg atctcctgaa gatataagga atatcttaag
     8881 gatactgcaa aatgatgagg ctgtgcatcg acgcagcgta aacgcatgta ctgagcggtg
     8941 aaattgccgg acgcagcggt gccttatccg gctaacaaaa aactaccagc gttttgccgc
     9001 ctgcagatcg ctctcccggg cttcaaccca gcggtcgcct tccggcgtgg cttcgcgctt
     9061 ccagaacggt gcgcgggttt tgagataatc cataataaac tgcccggctt caaacgcact
     9121 gctgcgatgc gcactggtga caccgacaaa aacgatttca tcgcccggcc ataattcccc
     9181 gatgcggtga atcaccgtga cgcgccccag cggccagcgg ttacgcgctt catcaacaat
     9241 ttctgccagt gctttttcag tcatccccgg atagtgttcg agggttaatg ctttgacgct
     9301 gtcgcccagg ttatggttgc gcacctttcc ggtgaaggtg actaccgcac cgtcttcgtc
     9361 acgctccgcc agccacgggt actcttctcc tacgctgaac ggctgcgggc cgacaacaat
     9421 tttggtttct gccatcttaa cctccggtta ccggcgggaa gaaagctact tcgtcgccgt
     9481 cagtcagcgg atggtcaaaa ctcaccagcg tctggttgac ggcagccagc aatttgccat
     9541 cttccagcgc cagcgcccag cgatcgctct gcgcagccag gtgctggcgt aacgcttcaa
     9601 cggttgggta atccgcagcc acttcggttg catctgttcc caccaactcg cgcacctggg
     9661 cgaaaaaaag aactttaatc atccgcttcc accttaaagt cacccgactt gccgccgctc
     9721 ttcgccagca aacgtaccgg accaatcacc atatcttttt gcaccgcttt gcacatgtca
     9781 taaatggtca gcgccgccac ggaggccgcg gttaacgctt ccatttcgac accggttttc
     9841 ccggtcaggc ggcataaggt ttctatacgc acccgattgt gctccggctc ggcctgtaaa
     9901 ttgacttcaa ctttgctgag catcagcggg tgacacagcg ggatcagatc ccaagtgcgt
     9961 tttgccgcct gaataccggc aatacgcgca gtggcaaata cgtcgccttt gtggtggcga
    10021 ccatcaataa tcatcgccag cgtctcgctg cgcatggtga caaaggcttc cgcccgcgct
    10081 tcacgcacgg tttccgcttt ggcggagaca tccaccatgt gcgcttcgcc agcggcgttg
    10141 atatgggtca gttgcgacat acttatttct tcaaatgtgg atggaaatta cacggacgcg
    10201 tacgggcatc cagctgcggc gcgatgatat tttcccatgc ggtacggcac gctttggtcg
    10261 aacccggcat ggcgaaaatc agcgttttgt tagcgacgcc cgctaccgca cgagattgca
    10321 acgtggaagt gccaatctct tcaaacgaca acatacggaa cacttcacca aaaccttcaa
    10381 cctcacggtc gaacaacggc agcagcgctt cgggagcctg atcaccttcc gtcaggccag
    10441 taccacccgt aatcaatacc acttgtacat cgtcgctggc gatccacgca gatacctgag
    10501 cgcgaatagc gtagcggttt tctttcacta tggcttcatc gacaacgtga tggcccgctt
    10561 cttgcgccga atcgcgcaga tagtgaccgg aggtatcgtc ttcttcaccg cgacgattag
    10621 aaaccgtaag aatagcaata cgggtcggga taaattcagt gcttacctga ctcatctgat
    10681 ctctcctttt gacgttttag ccgccaatgt acgataagtt ttgcgtaata ccggtgttgt
    10741 tttgatgcag gaaatgggtc tgtttcttct cccgcagcgc cgctgaaata cgcgcttcca
    10801 gcgcctgttg ctgggtatcg ccttccagca gatcgcgcag gttaacgccg ccttcaccaa
    10861 acaggcagag atggagttta ccaatggagg aaacgcgcag gcggttgcaa gtggcgcaga
    10921 agtctttttc atacggcatg ataaggccaa tctctccggc gtaatctgga tggcaaaaga
    10981 cttgcgcggg accgtcgctg cgttgacgta attggtggat ccagccgcga cgcagtagct
    11041 cgtcacgcag aacctgacca gagatgtgat gcttacggaa gagctcgctg ccctcgcccg
    11101 tttccatcag ttcgatgaaa cgcagctgga tagggcgatg ctggatccag ttcagaaagg
    11161 tgtcgagctg gtgatgatta acatcacgca tcagcacggt attgactttg accttctcaa
    11221 aaccggcctc aaatgcagca tcaatccctg ccatgacctg gttgaattta tcctgcccgg
    11281 taatagcgtg aaactggcgg gcgtccaaac tgtcgacact gacgttaatg ccagtaagtc
    11341 ccgcatcgcg ccagttcgcc acatcgcgtt ccagacggta accattggtg gtgaccgcaa
    11401 tctggcggat agcgtcgttt tcccgcacag cggcgatgat atcggtaaag tcgcggcgta
    11461 aagacggctc tcctcctgtc aggcgcactt tttcggtgcc cagactggcg aaggcgcgcg
    11521 taacccggcg aatttcatcg acggtaagaa agcctttatt ggtgacgccg ctcggtttgt
    11581 agccatccgg caggcagtag gtgcaacgaa agttacacac atcggtaatc gacaggcgca
    11641 agtagtaaaa cttacgcgca aatacatcag tcagttgtga agccatgtac acctttccag
    11701 atacgggagg cgaagtcatt tcttccttcg ccctggtggc aatctttacg agcattgtca
    11761 cggccaaagc accgtatcag ttgacccagg tgcagaggct agagtgttta gtggttaagc
    11821 cgatactagc gtgtaaatga gatttttacc atccacactt tcgctatgta atcatgtata
    11881 tagcgtcatg atcgtgctat ttcgctacag aataagggaa aatggcggtt ttactttgat
    11941 atacatcatc agggaggcat agcgtgcatc gtcctttaga gatagaagaa aatagtttgt
    12001 atcctcaata ttggcaggtt aattgctgtt tccccgcaat ttgcgctact gtagcgcgga
    12061 taatctgact ccaggaaact atatgcgcaa tcgtacgctg gctgatcttg atcgtgtcgt
    12121 tgctctcggc ggagggcatg gactgggacg cgttctctca tcactttcgt ctttgggttc
    12181 tcgtttaacg ggtatcgtca ccaccacgga taatggtggc tcgacggggc gtattcgccg
    12241 ttcagaaggc ggcattgctt ggggcgatat gcgcaactgc ctcaaccagc tgataacgga
    12301 accgaacgtc gcctccgcga tgtttgaata ccgttttggc ggcaatggcg aactttccgg
    12361 tcataatctc ggaaacttga tgttaaaggc gctggatcac cttagcgtgc ggcctctgga
    12421 agccatcaat ttaattcgta atctgctgaa agtggatacg catttgattc caatgtcaga
    12481 gcatcctgtt gatctgatgg cgattgacga tcaggggcat gaagtttacg gcgaggtcaa
    12541 tatcgaccag ttaactacgc cgattcaaga gttattgtta acgcctaatg tacccgcaac
    12601 gcgtgaggcg gttcacgcta tcaatgaagc ggatctcatc attattgggc ctggcagttt
    12661 ttataccagc ctgatgccaa ttctgctgct gaaggaaatc gcccaggcat tacgccgcac
    12721 gccagcgccg atggtttata tcggcaatct ggggcgtgag ttgagtttac ctgcggctaa
    12781 tttgaagctg gaaagcaagc tggcaattat ggagcagtat gttggtaaaa aggtcattga
    12841 tgcggtcatc gtcgggccaa aagtggatgt ctcggcggtg aaagagcgga ttgtgatcca
    12901 ggaggtactg gaggccagcg atattccgta tcgtcatgac cgccagttgt tacataacgc
    12961 gctggaaaag gcgttacagg ctttaggtta acggaaatcc gaaggaaaat tccggcttcc
    13021 tattgaagac aaagtgcgcg ttgtttatgc cggatgcggc gtgaacgcct tatccggcct
    13081 acaaaccgcg caaattcaat atattgcgga gaaaatgtag gcctgataag cgtagcgcat
    13141 cagactgttt tccgtttgtc atcagtcttc ttcgctatcc tgttacgatg cggcaataaa
    13201 caactcacgc aactgatgca actggtcacg aatttgtgcc gcttcttcga actccagatt
    13261 ctgcgcgtgt tgcatcatca gcccttccag ttcatggatt ttctgctgca acgcttccgg
    13321 caacatatcc atcggcacat tatccggctc aacaatcggg cgcgattttc ctctgccctt
    13381 cgctttggtt ttggcaatgt tctgccccag cgccaggata tcgaccactt tcttgttcaa
    13441 gccttgcggc gtaatgccgt gttcctcgtt gtacttctgc tgtttctcac ggcgacgttc
    13501 ggtttcgcca atcgctttcg ccattgatgg agtgctccta tcgccgtaga gaatcgcttt
    13561 accgttaacg ttacgtgccg cacgaccaat ggtctggatc aacgaacgtt cggaacgcag
    13621 gaagccttct ttgtcagcgt cgaggatcgc caccagcgac acttccggca tatccagacc
    13681 ttcacgcagt aagttgatcc ctaccaatac gtcgaactca cccagacgca agtcgcggat
    13741 aatctccata cgctcgacgg tgtcgatatc tgagtgaaga taacgcacgc gctcaccgtg
    13801 ttcttcgaga tattcggtaa gatcttccgc cattcgcttg gtcagtgtgg tgaccagcac
    13861 gcgttcgtta attgccgctc gctgacgaat ctccgaaaga agatcatcaa cctgtgtcgc
    13921 caccggccgc acttcgataa tcgggtcaag caatccggtt ggacgcacca cctgatccac
    13981 cacatcgccg ccggattttt ccagctcgta attacccggc gtcgccgaaa catagatggt
    14041 ttgcggcgct aatgcttcga actcttcaaa cttaagcgga cggttatcca gcgctgatgg
    14101 caggcggaag ccgtactcca ccagtgtctc tttacgcgcc cggtcaccgc gatacatgcc
    14161 gccaatttgt ggaatggtga cgtgagattc atcgacgacc agcagcccat cggcaggcag
    14221 gtaatcaaac agcgtcggcg gtggctcacc cggtccacga ccggagagga agcgcgagta
    14281 gttttcaatc cccgaacagt agcccagctc gtccatcatc tccagatcaa actgggtacg
    14341 ctgggtcagc cgctgctctt ccagcagttt gttgttttcc aacagcactt tgcgtctggc
    14401 ggccagttct tctttgatct cctccatcgc ctgtacgatg cgctcgcgcg gtgtgacgta
    14461 gtgcgttttc gggtagatgg taaaacgtgg aatagtggaa acaatctgcc cggtcagcgg
    14521 gtcaaataac gacaatcgtt ccacttcctc gtcaaacagt tccacgcgaa gtgcaatgtc
    14581 atccgattct gccgggaaga tatctatcac ctcgccacga acgcggaaag taccacgctg
    14641 gaatgcttga tcattacgag cgtattgcag ctccgccagt cggcgcagaa tcgcgcgctg
    14701 atcgataatc ataccgaccg tgagatggag catcatcttg agatataaat caggatcgcc
    14761 cagaccataa atcgcggaaa cagacgccac tacaatcaca tcacgccgct ccagcatcgc
    14821 tttggtggcg gacaaacgca tctgctcaat atgttcgtta accgaggcat ctttctcaat
    14881 gaaggtgtcg gaactcggta cataggcttc cggctgatag tagtcgtagt aggagacgaa
    14941 atactccacc gcgttttccg ggaagaactc tttcatttcg ccatacagct gggccgccag
    15001 cgttttgttg ggcgcaagta ccatggttgg gcgctgaagg tcagcaatga cattggcaat
    15061 ggtgaaggtt ttcccggagc cagtcacgcc aagtaacgtc tggtgcgcca ggccatcttc
    15121 cagcccctct tcgagacgtc gaatcgcctc tggctgatcg ccagaaggtt taaaagcgga
    15181 attcagtttg aacggtttac tcatgagtcg ctacctgaag gagttgggcg ggcaggtatg
    15241 taattttact cgtcgtactt aattatgcca acaaattata ctggataaaa aaacagttca
    15301 tcaccataat atttctgata cagcgtaaac tccgctgtca atcatgagca aaatttactc
    15361 tgtggcgaga taaaactcca gccttaccgg gttatcccca aagcaacggc ttttttaaca
    15421 tttgtcaaga tgagtgatga cagttttatg gcaagagatg cctgttcagt gactgccatt
    15481 gcatttatct aaccagttaa aaaataaaag atatttcttt gagccgtctt taacgccaat
    15541 tcgcgtgcaa gccgcgtatt ctctcgcttg cctcgtgttt tctaactcta atacacatgg
    15601 ttatccacag gaatagtgga taactgtctc cagcccctat cccccgccgc ctgggcaatt
    15661 cccacattcg ccgcatgagg cggaagtaaa atttttttcg tgattatttt agaatttaat
    15721 tggttaaatt gcagtcaatc gaagacgcga tctcgctcgc aatttaacca aatacagaat
    15781 ggctacaaca aggcaaggtt tatgtacttt ccggttgccg cattttctgg attttctgca
    15841 agccagggga tctctcccag cagcggcgcg ggaatcatgc gggtgagcgt ggtcatatat
    15901 tcagcgtgac gttttcccgg aggcgtaaca tcgttcgcca cccaacccgc cagagtcagt
    15961 ccggcgtgtt gtattgcctg cgcagtcaac atcgcgtgat taatacagcc gagtttcaca
    16021 ccaactacca gtatcaccgg cagttgttcc tgtgttaccc aatctgcaaa agtgaaagtg
    16081 tcagaaagcg gcgtaaacca gccgccagca ccttccacta acacccagtc agcctgttgt
    16141 tcaagcgcgc gtaatccggc gctcattacc aatgattcta tcggcctgcc ctcttgggcg
    16201 ctgatgatgt gcggcgaggt ggattctgcg aaggtgtaag gatttaccgt tgcgtaatcc
    16261 agctgcaggc tgctgttgtg ctgtaacgcc agcgcgtcac tattacgtaa gccttccggg
    16321 gtcttttcgc tgccagaggc gaccggttta taacctgccg tccgataacc tactgccttc
    16381 gcggcttgta aaagtgcaca actggcgacg gttttcccca cttcggtatc cgttccggtg
    16441 acaaaataac gtttactcac gagcaatcac tcccaaaaaa agatgatacg tcagaggata
    16501 tcgcccctgt tgttgcggcc aggccagttg caatcgctgc aactgcgaac gcgttaatat
    16561 tcgcgggtcg cgcccttcat gaagatgcgt agcaccgatg cctttcagcg aacgcatggc
    16621 actgagcgca tcatcaaacc atagtgtgat gagctgaata tgatgttgat aacgcacgcc
    16681 gttcagcgac tcttcgattt catctggcgg taaaaagcga ttagcatgcg gacgctcgtc
    16741 caccgcctgc cgcgcctgat gcagttcggg taacgatccc tgcaccagcg tggtaaacgc
    16801 gaccacgcct ccggggcgca ccacccgata cagctcgcgg agtgccgtgg ataaattacc
    16861 gcaccactgc accgcgagat tgctccatgc aagatcgaac gtcgcagtcg ctaacggcag
    16921 ggattcgata tctcctgcca gataatgatc tgcggcatct ttatggcggg cttgcgcaag
    16981 cattggcggc gagagatcta acgccgtcac ctgcgcgtga cgttcccgcc agtggcggct
    17041 catccagcca ggtccacaac ccgcgtccag tacgtgggtg tatttacgct gtggaagcat
    17101 tgccagtaag gcgtcagcac tctggcgctg tagatctgca tgttgctcat agtgtgcggc
    17161 tgcccgacca aatgccgctg caatggcttg tttattaacc gttgccatgc agcacctcca
    17221 gcagacggtc gatatcctgc atttcatgcg cagcggttag cgttaagcgc agtcgcgcag
    17281 taccagcggg tacggttggc gggcgaatcg ccgtgaccca gcagccttgc tggcgcagtt
    17341 tttctgccag ttgtaacgca cggctgttat caccgacgat caatggctgg atggcgctgc
    17401 atgaatcagc cagcgtaaac ggcaaatcct gtactccggc acgaaaacgc gcaatgagtg
    17461 acaccagttt ttcgcgccgt gcatccccct catcacggcg aatgaccgcc agcgacgcac
    17521 gtaatgcctg cgcctgagcg ggcggcatac tggtgctgta gataaggtgg cgggcgaatt
    17581 gcagcagata atccgccacc gtacttgagc aaagcaccgc agccccactg acgccaaatc
    17641 ctttgccaaa agtcactacc agcaattctg gttttacctt ttgcagccag cagctgccgc
    17701 gcccctgctc cccgataacg cccgtgccgt gggcatcatc gaccatcaac cagccattgt
    17761 gctgttgcgt tacctgctgg atttccgcca gtggcgcgct atcgccgtcc atgctgaaca
    17821 cgccttctgt caccaccagt tgctgccccg gacaggggga agcaagcagt cgcgccagat
    17881 gagcgacatc gttatgagta aaacggcgaa gctgcgacgg gcttaaactg gcagcctcca
    17941 gcaatgaggc atggctaagc cggtcggcaa caatacggtc ctctttcgcc atcatcgcgg
    18001 caatgactgc ctgattcgcg gcaaaaccag agataaacag cagcgcccgc gaatagccaa
    18061 gccactcggc cagttcttct tccagcgcct gatgcaccac gctataaccg ctgacgtgac
    18121 cggagccgcc gctaccgatg ccaaattgct ccgccccctg cttccaggca cggataattt
    18181 gcggatgatg gcttaaacct aaataatcgt tactggaaaa gttcagatac tggcgatcat
    18241 ccgccaccag ccagcgtccg gctccttgcg ccaccggata acggcgacgc agggcatcgg
    18301 cagcacgccg cgcatcgagc gccgcgttga ttttctcctg ccagctcata atgctgccgc
    18361 gttgtaatat tcgtcggtgt ccggggtcat cagcgcctgt ccaagacgtt gctgttgttc
    18421 gttatcccct gccagcacgg cagtttgctg cggatttagc cccagtttgc ggaacagttg
    18481 caggtcttta tcttcttccg gattcggcgt ggtcagcagt ttgcaaccgt agaaaatcga
    18541 gtttgcgcct gccataaagc acatcgcctg agtctgttcg ttcatctgct cgcgtccggc
    18601 agaaaggcgc acgtaagagg tcggcatcat gatccgcgct acggcaatgg tgcgaataaa
    18661 atcaaaggca tcaacgtcat cgttatcggc aagcggcgtg cctttcacct tcaccagcat
    18721 gttgattggt acgctttccg gcggcgtcgg caggtttgcc agttgcagca ataatccggc
    18781 gcgatctttt accgtttcgc ctaagcccac aatgccacct gagcagactt tgatcccggc
    18841 atcgcgcact ttttccagcg tatcgaggcg ttcctgataa gtgcgtgtgg tgatgatatt
    18901 gccgtaaaac tccggcgagg tgtccaggtt gtggttgtag taatccagcc cggcgttcgc
    18961 gaggcgctgc gcctgagatt cactcaacgt gcccagcgtc atacacgcct ccagccccat
    19021 cgcttttacc ccctgcacca tttgttccag gtacggcata tcgcgttcgt ggggattctt
    19081 ccatgccgcg cccatacaga agcgcgtcga tcctgccgct ttcgctttgc gcgccgactc
    19141 cagcacctgt tcaacttcca tcaaccgctc ggcttccagc ccggttttgt agcgcgagct
    19201 ttgcgggcag tatttgcaat cttccggaca agctccggtc ttaatcgaca gcaacgtgct
    19261 gacctgcacc tgacgaggtt cgaaatgctg gcgatgcacc tgctgcgctt caaacagcag
    19321 atccagcaac ggtttttcaa ataattctgt gacttgcgac aatgtccagc gtgggcggtg
    19381 agccatgggg cttctccaaa acgtgttttt tgttgttaat tcggtgtagg cttgtaaacc
    19441 taaatctttt caatttggtt tacaagtcga ttatgacaac ggacgatctt gcctttgacc
    19501 aacgccatat ctggcaccca tacacatcca tgacctcccc tctgccggtt tatccggtgg
    19561 cgagcgccga aggttgcgag ctaattttgt ctgacggcag acgcctggtt gacggtatgt
    19621 cgtcctggtg ggcggcgatc cacggctaca atcacccgca gcttaatgcg gcgatgaaga
    19681 cgcaaattga tgccatgtcg catgtgatgt ttggcggtat cacccatgcg cccgccatcg
    19741 agctatgccg caaactggtg gcaatgacgc cgcaaccgct ggagtgcgtt tttctcgcgg
    19801 actccggttc cgtagcggtg gaagtggcga tgaaaatggc gttgcagtac tggcaagcca
    19861 aaggcgaagc gcgccagcgt tttctgacct tccgcaatgg ttatcatggc gatacctttg
    19921 gcgcgatgtc ggtgtgcgat ccggacaact caatgcacag cctgtggaaa ggctatctgc
    19981 cagaaaactt gtttgctccc gccccgcaaa gccgcatgga tggtgaatgg gatgaacgcg
    20041 atatggttgg ttttgcccgc ctgatggcgg cgcatcgtca tgaaatcgca gcggtgatca
    20101 ttgagccgat tgtccagggc gcaggcggga tgcgcatgta ccatccggaa tggttaaaac
    20161 gaatccgcaa aatgtgcgat cgcgaaggta tcttgctgat tgccgacgag atcgccaccg
    20221 gatttggtcg taccggcaaa ctgtttgcct gtgaatatgc agaaatcgcg ccggacattt
    20281 tgtgcctcgg taaagcctta accggcggca caatgaccct ttccgccaca cttaccacgc
    20341 gcgaggttgc agaaaccatc agtaacggcg aagccggctg ctttatgcat gggccaactt
    20401 ttatgggcaa tccgctggcc tgcgcggcag caaacgccag cctagcgatt atcgaatccg
    20461 gcgaatggca gcagcaggtg gcggctattg aagtgcagct gcgcgagcaa ctggcaccag
    20521 cccgtgatgc cgaaatggtt gccgatgtgc gcgtactggg ggcaatcggt gtggtcgaaa
    20581 ccactcgtcc ggtgaatatg gcggcgctgc aaaaattctt tgtcgaacag ggggtatgga
    20641 tccggccttt tggcaaactg atttacctga tgccgcccta tattattctc ccgcaacagt
    20701 tgcagcgtct gaccgcagcg gttaaccgcg cggtacagga tgaaacattt ttttgccaat
    20761 aacgggaagt cagcgtgagg gtttctggct acactttctg caaacaagaa aggagggttc
    20821 atgaaactca tcagtaacga tctgcgcgat ggcgataagt tgccgcatcg tcatgtcttt
    20881 aacggcatgg gttacgatgg cgataatatt tcaccgcatc tggcgtggga tgatgttcct
    20941 gcgggaacga aaagttttgt tgtcacctgc tacgacccgg atgcgccaac cggctccggc
    21001 tggtggcact gggtagttgt taatttaccc gctgataccc gcgtattacc gcaagggttt
    21061 ggctctggtc tggtagcaat gccagacggc gttttgcaga cgcgtaccga ttttggtaaa
    21121 accggatacg atggcgcagc gccgccgaaa ggcgaaaccc atcgctacat ttttaccgtt
    21181 cacgcgctgg atgtagagcg tattgatgtc gatgaaggag ccagcggcgc gatggtcggg
    21241 tttaacgttc atttccactc tctggcaagc gcatcgatta ccgcgatgtt tagttaatca
    21301 ctctgccaga tggcgcaatg ccatctggta tcacttaaag gtattaaaaa caactttttg
    21361 tctttttacc ttcccgtttc gctcaagtta gtataaaaaa gctgaccgag aaacagtaaa
    21421 aatcattaat atcaatgcat tataatacat ttagcttaaa aaatagactg cataatactg
    21481 caaaacacaa catatccagt cactatgaat caaccactta gatggtatta gtgacctgat
    21541 acagagcatt agcgcaaggt gattttttgt cctcttgcgc taattttttg tcaacgcact
    21601 gggttagctg aatttacaac catgctcagt atctcgataa gcgcagagaa atgatgctgt
    21661 ggtgggcaga ctactggctt gatgaaaagg tagggcgacc aaccgcgttt tttcagttca
    21721 gtcagcacat tgagccagaa cttcacccct tcattttcgg ccagccacat ccccaacagt
    21781 tcttcctgtt cttaactatt tacaggcgcg tttttttaaa gaatcctcac tccatttgca
    21841 cgaatatatt aatatataaa caatgaggat caataataaa tgaaacctgc ccacaatcct
    21901 tcttttttcc gctccttttg tggtttagga tgtatatccc gtttatccgt agaagagcaa
    21961 aatatcacgg attatcaccg catctgggat aactgggcca aggaaggtgc tgcaacagaa
    22021 gaccgaacac aggcagttcg attactgaaa atatgtctgg cttttcaaga gccagccctc
    22081 aatttaagtt tactcagatt acgctctctc ccatacctgc ccccgcacat acaagaactt
    22141 aacatctcta gcaatgagct acgctctctg ccagaactcc ctccgtcctt aactgtactt
    22201 aaagccagcg ataacagact gagcaggctc ccggctcttc cgcctcacct ggtcgctctt
    22261 gatgtttcac ttaacagagt tttaacatgt ttgccttctc ttccatcttc cttgcagtca
    22321 ctctcagccc ttctcaatag cctggagacg ctacctgatc ttcccccggc tctacaaaaa
    22381 ctttctgttg gcaacaacca gcttactgcc ttaccagaat taccatgtga actacaggaa
    22441 ctaagtgctt ttgataacag attacaagag ctaccgcccc ttcctcaaaa tctgaggctt
    22501 ttaaacgttg gggaaaacca actacacaga ctgcccgaac ttccacaacg tctgcaatca
    22561 ctatatatcc ctaacaatca gctgaacaca ttgccagaca gtatcatgaa tctgcacatt
    22621 tatgcagatg ttaatattta taacaatcca ttgtcgactc gcactctgca agccctgcaa
    22681 agattaacct
//
LOCUS       DAZSNM010000048        22099 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZSNM010000048 DAZSNM010000000
VERSION     DAZSNM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22099
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.048"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..841)
                     /gene="focB"
                     /locus_tag="ACHW42_001559"
                     /pseudo
     CDS             complement(1..841)
                     /gene="focB"
                     /locus_tag="ACHW42_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(863..2875)
                     /gene="hyfR"
                     /locus_tag="ACHW42_001560"
                     /pseudo
     CDS             complement(863..2875)
                     /gene="hyfR"
                     /locus_tag="ACHW42_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            complement(2905..3318)
                     /gene="hyfJ"
                     /locus_tag="ACHW42_001561"
     CDS             complement(2905..3318)
                     /gene="hyfJ"
                     /locus_tag="ACHW42_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR8943431.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(3311..4069)
                     /gene="hyfI"
                     /locus_tag="ACHW42_001562"
     CDS             complement(3311..4069)
                     /gene="hyfI"
                     /locus_tag="ACHW42_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR8943432.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(4066..4611)
                     /gene="hyfH"
                     /locus_tag="ACHW42_001563"
     CDS             complement(4066..4611)
                     /gene="hyfH"
                     /locus_tag="ACHW42_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR8943433.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(4621..6335)
                     /gene="hyfG"
                     /locus_tag="ACHW42_001564"
                     /pseudo
     CDS             complement(4621..6335)
                     /gene="hyfG"
                     /locus_tag="ACHW42_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            complement(6325..7911)
                     /gene="hyfF"
                     /locus_tag="ACHW42_001565"
     CDS             complement(6325..7911)
                     /gene="hyfF"
                     /locus_tag="ACHW42_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR8943434.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            complement(7916..8566)
                     /gene="hyfE"
                     /locus_tag="ACHW42_001566"
     CDS             complement(7916..8566)
                     /gene="hyfE"
                     /locus_tag="ACHW42_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR8943435.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8578..10017)
                     /gene="hyfD"
                     /locus_tag="ACHW42_001567"
     CDS             complement(8578..10017)
                     /gene="hyfD"
                     /locus_tag="ACHW42_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFR8943436.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(10034..10981)
                     /gene="hyfC"
                     /locus_tag="ACHW42_001568"
     CDS             complement(10034..10981)
                     /gene="hyfC"
                     /locus_tag="ACHW42_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR8943437.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10992..13009)
                     /gene="hyfB"
                     /locus_tag="ACHW42_001569"
                     /pseudo
     CDS             complement(10992..13009)
                     /gene="hyfB"
                     /locus_tag="ACHW42_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(13009..13626)
                     /gene="hyfA"
                     /locus_tag="ACHW42_001570"
     CDS             complement(13009..13626)
                     /gene="hyfA"
                     /locus_tag="ACHW42_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR8943438.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            complement(13879..14349)
                     /gene="bcp"
                     /locus_tag="ACHW42_001571"
     CDS             complement(13879..14349)
                     /gene="bcp"
                     /locus_tag="ACHW42_001571"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR8943439.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14349..14921)
                     /locus_tag="ACHW42_001572"
     CDS             complement(14349..14921)
                     /locus_tag="ACHW42_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR8943440.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15067..15945
                     /gene="dapA"
                     /locus_tag="ACHW42_001573"
     CDS             15067..15945
                     /gene="dapA"
                     /locus_tag="ACHW42_001573"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR8943441.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15962..16996
                     /gene="bamC"
                     /locus_tag="ACHW42_001574"
     CDS             15962..16996
                     /gene="bamC"
                     /locus_tag="ACHW42_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR8943442.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17209..17922
                     /gene="purC"
                     /locus_tag="ACHW42_001575"
     CDS             17209..17922
                     /gene="purC"
                     /locus_tag="ACHW42_001575"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR8943443.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18090..18953
                     /locus_tag="ACHW42_001576"
     CDS             18090..18953
                     /locus_tag="ACHW42_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR8943444.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            18968..20983
                     /gene="tmcA"
                     /locus_tag="ACHW42_001577"
     CDS             18968..20983
                     /gene="tmcA"
                     /locus_tag="ACHW42_001577"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR8943445.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            21057..21755
                     /gene="ypfH"
                     /locus_tag="ACHW42_001578"
     CDS             21057..21755
                     /gene="ypfH"
                     /locus_tag="ACHW42_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR8943446.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(21865..22065)
                     /locus_tag="ACHW42_001579"
     CDS             complement(21865..22065)
                     /locus_tag="ACHW42_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR8943447.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
BASE COUNT         5555 a         5839 c         5955 g         4750 t
ORIGIN      
        1 aacaaatact caccagcact gcaccgccga taatattacc cagcatcacc gggagcagat
       61 tggcggtaat aaaatggctg accgtcagtg ccggaaaatt gtctgcgcta ctgtgcgcca
      121 gttgccagaa ggaggtaggg gcgaaatggc gaatggcaat ggcgaacgga atcacaaaca
      181 aattggcgat acagtgctca aagccactgg cgacaaacag ggtgatggac aaaatcatgg
      241 cgacgatttt gtcgcataac gaacgtccgc aataactcat ccacagcgcc aggcaaacca
      301 tcagattgca cataatgccg aggctgacag attcagtaaa tgtatgatgc attttgccct
      361 cggcgcagtg taaaatcgcg actccccaca tcgcgttttc actcatcacc agcccggaaa
      421 accagattaa caaactgaaa caggcaatac ctgccagatt accgcaggcc accagaagtg
      481 cgttaatcag ccaagttcgc caactaataa cgccccgact ttttgccatt accgtcatta
      541 ccgacgaggt gaacaggctg gtgccgcaaa ccgccagcaa aataaagcca agtgtaaagc
      601 aaaggccgcc caccagatgg gttaatgcct atgaagacgg ggcatcggcg ataacggaaa
      661 ggtaaaaagt aaatccgatc gccataaaca cgccagcgga catcgccata aacacgccag
      721 cggacatcgc cattaaaaag accgacactt tactgcgggc aattttatgg gcggcaatcc
      781 gttcagcgat tgccgctttt ctggcgctca actgcaagtc gaaagagagt ttgtttctca
      841 tgcatactcc tgcaaaagca gattacaaca cctcgcgaac cgagattccc agacgctgca
      901 ttcgtgacag cagcgtggtg cgcttcatcc ctaatcgtgt cgccgcgcca cggggcccgg
      961 caacaatgcc attggtttct cgcaatacct gaacaatgcg ctgacgttct tcttcgtcat
     1021 tctctggcgt cgtcgggtgc agcaactacg ccattgaact gcgaagcgct gaatcttcat
     1081 ttagcgtcgg cagtaaacgg ctttgtcgga cattaagatg taaattcaga ctgttaccgc
     1141 gagtcagcag taccgctcgc tcaatcacgt tttccagctc gcgcacgttg cccggccagt
     1201 cccacgacat caactggcgt agcgcctcgg tcgggatggc gtcaattgaa cgattcatat
     1261 ggcgcgccat tttttgcgtg aaatgctttg ctaaaagagg gatatcttcc ggacggtcgc
     1321 gcagcggagg caattccagt gggaagacat tcaggcgata aaagagatcg ctgcgaaact
     1381 ggcgatcttc aaccatttgc cacaaatcac ggttggtggc ggcaatgact ctgacattta
     1441 ccgggatcgt tctactcccg ccgagacgct caatctcccg ttcctgcaat acgcgcagca
     1501 gtttaggctg aagttctaac ggcagatcgc caatttcatc gagaaacaac gtgccgccat
     1561 cggcaatttc aaaacgacca cgatgggtat taatcgcacc agtaaacgcc cctttgtcat
     1621 gaccgaataa ctcactttcc agaagactgg cggggatggc agcgcagttg attttgacca
     1681 gcggcttgtc gtgtcgcggg ctaagttgat ggatcgctct ggcgatcacc tctttgccgg
     1741 ttccggtttc accgcaaatc aacaccgtac tgtcgctctt cgccacaata tctacctgct
     1801 ggagcaggtc ttccattgcc tggctttgat agataatgtc accgacgccc agattcgaaa
     1861 ggagctgctc gctaagctgg tggttttctt gctgcaaact ttcctgcaaa tcggtcattc
     1921 tacggcaggc atcggcattg ccaacggcaa tagcgatgcg atcggctatg tgttgtagta
     1981 gctggcagtt ttcctcacta aagagagtgg aagaggtatg cgccagcaac aatgcacccg
     2041 gtgtatggtt gccaaaggta agcggtataa ggagcgcaga ttcgcagccg ttagatgcaa
     2101 gtaagagtaa taacgggtcg cgttgccaga gaaacagatc atctgcacgg tgcgtcaagg
     2161 tcggttgtcg ggtttgtagc gtttgtgtca gcaatacact gtcgccaggc atattgtgtg
     2221 gcagacacgc acaatgtgag gcagaaagat cgctgcacca caggctgaac ttctcgttct
     2281 ttcgatgatc gcccagtacc atactgactg aagccagacc gaaaaaatga tggatctcac
     2341 gagcgacgtc agcgatcaga tcgtcgagat caagatgtga cagcactgca ttagtgatat
     2401 ccaccagtac ccgatgatga tcgcgatctt tctccagcaa acgcagcgcc acctgctgtg
     2461 cttcatcatt gagccatgat tgcagaaccg gggtgataat gcgtgccagc cagttaaaca
     2521 gtgtgcaatc ctggggagta aaacggctgc cgtgggtacg aataaagcgt agctccgcca
     2581 ttccactact gccgagaagg acggcgtaac gcgaataatc gaccatctcg ccctgatact
     2641 ctgttgttgc cgtcacatcg ctgccatcat catggtagcg gtaatagaag ttgcttttga
     2701 gaacatgcag ttcaataagt tgtaccgggc tgaacggcgg cggcagcggg atgaaggcgc
     2761 gtaataaaga cgccttgccg tgtttctgcg ctaaccgctc cgcgagcatt ccgtttaccg
     2821 cttcaattgt tattccttgc ggcggggcaa acatcgcttc gtctgacata gccatcaaca
     2881 gtccttatta atgagaaatc cttctcacgc aatttttctg gccatcagat agatagccgg
     2941 ttccagcaca atttcatcca acagatgaat gaattgaatg ctacgcgctt gccacacaga
     3001 cgtcgcgtca tctgccagtg gcgcgaatgc ccgggtcaac agggcggtgt ggctggcgtc
     3061 aataacaatc tcaccaaaag tcatcacccc cagcatctta cgtcgggctt gctcctcttc
     3121 gaccagtgca agccaatctt cgtattccgt cagtgggcag cggaaggcga cattcagaca
     3181 atcaatcacg ccaacgtgat gaccgatagc cagcgagtaa tacattacct gagagctgtg
     3241 ttccggcatc tcgtcgctac tggcgacaaa cttttttcgc agcgtccaga aaacaatttc
     3301 cccgcactct tcagtcatgt aacccccgga gtacgcgaaa gagttgctga acgatactat
     3361 tgagacgtgg atcgtcggca tcgcgcaacc aggtgttaac ccgatttcct gtaggatcgt
     3421 cgcttaaatg gcgcaggagc cgatcgcaaa tttctcgccc ctgacgatag cccgccagcc
     3481 gccgcgcttc tcgctcaatg gcgatacgct gtgatggcgg gatctgcggc cacaacggtt
     3541 gcatagtcac cccggtggga tcgcgataat ccaccgcgtg aatcttctgt tgcagcaaac
     3601 cgagcgccac ggcgaaaccg tgaatggtgg cggccggtgt tggcgggcag ccggggatcc
     3661 aaacatcaat ggggacaatg gtgtcgctgc cgccccagac gctgtagaga tcgtggaaaa
     3721 taccgccgcc gacaccgcac gcgccgtagg aaacacagat tttatgatcg ggggcagact
     3781 catacgcccg aagtgcaggc atacgcatcg cccgggtgac tgcgccagta aataacaaaa
     3841 tatcggcgtg acgcggtgat gaaacaacct taatgccaaa acgttctgcg tcgaatacgg
     3901 gggtgatggc agcaaaaatt tcgatttcac aggcgttgca gccgccgcaa tcgatgcgat
     3961 aaacgtaagc cgagcgacgg atatcctgta gcaaatgccg cttcatcttt tgcgtttgct
     4021 cgtccagcgt gatgggctgg ctgacatatt gcgtaagcac tggactcata gctgctcctt
     4081 agccaccagc ggtacatctg tatcgtcgtt gatcagcgtt gcgcgttgtt tgcactccgg
     4141 gcagacgctc gcttgcgccc ataacatttc gcggttttgt ggagcatttt gttgctgtgc
     4201 caacaattca gcagccagtg cgacggtttt ttgcggggca aacgggcgtt cgcaacggct
     4261 gcaacgttgt agatggaacg tcgcgtgggt atagagatcg gctttatggg tgacggtcag
     4321 ttcaaagtta ttggtaagct ggatggctct ggtcgggcac acttcttcac aacgtccgca
     4381 gtaaatacaa cgccccagat agagctgcca ggtgcgcgaa ttttgctggt cgtcggtctg
     4441 gatagtcagc gcatttgccg gacaagcaca ggcgcaggca ccgcaggcaa tacattggct
     4501 gggcatcagg tccggtttcc cgcgaaagcc tgggctgacc tccagtggcg cgaagggata
     4561 tttcaccgtc gcggttccgg cgcgcataat agttttcagt aacttcagca tcttctgtcc
     4621 ttacttcagc ggcgaacggt tacgatcaat gccgtagcgt tcgatctctt tatacagcac
     4681 ggtttttgac tggcgcttgc gcacatctac cagcgtcaca cggtcggtac aggagtagca
     4741 gggatcaagg ctaccgataa tcagcggtgc gtcagaaacg gtattgccgc gcagcatgta
     4801 acgcaacacc ggccagttgg cgtaggtggc ggcacgacag cgccagcgga acaatttttg
     4861 gttatcaccg agcatgctcc agtgcacgtc ttcaccgcgt ggcgcttcaa caaagcccag
     4921 cgcgaatgcg tgaggtttat agctaaagcc ttcggtcagc agtggggtat ccggcatgtt
     4981 gtcgagggca aattccagca ttgccagcga atcaaacgtc tctttgacac ggaccatcac
     5041 gcgggagaaa acatcgccgc cggtaaaggt aaacagtgtt tttggaatat tgccgtagtc
     5101 ggcgtagggg tgatcaaagc gcaaatcacg ggcaaaacca ctgcgcggat cagcggccct
     5161 acagggctat aatcacgggc gatttgtcgg tcgagaatgc caatgccctg agtgcgttgt
     5221 tccatattcg gcgtagcaag cagcatctct accagctccg acacgtcggc gcgcatctcg
     5281 cgcaccagtt tcagcgtttg cagacgttgc tctttgagaa tatcgcggcg aacaccacca
     5341 atcagattca gaccgtaggt tttacgcgac ccggtcagca attccgccat cgtcatcgac
     5401 ttttcccgca cgcggaaaaa ttgcataaag ccggtatcaa aaccaacaaa atggcaggag
     5461 aggccaaggt tgagcaaatg actgtgtagc cgctcgactt ccagcagaat cgagcgaata
     5521 gtatgtgctc gttgcggcac ctcaatcccc agtgcatttt caaccgagtt ggtataggcc
     5581 acactgtggg caaaaccgca aatcccacac acgcggtccg ataagaaggt cacttcgttg
     5641 tagcccatcc gcgtttctgc cagtttctcc atgccgcgat ggacataaaa caggcggtaa
     5701 tcagcatcga caatttgctc gccatccaca aacaagcgga agtgacccgg ttcataggag
     5761 gtgatatgca gcgggccgac agggatcacc cgcgcatcgc tgttgccctc attgataaac
     5821 gcatacgttt cggaatctgt cgtcggttca gggcgcagtc gataatccat cgcatctttg
     5881 cgcagcggat gcatatcttc cggccagtca tcgggcaaca ccaggcgacg ctgatccggc
     5941 aggccaaccg gaatcagccc gtacatatcg cgaatttctc gctcgcccca gaccgcggca
     6001 gggacgcgcg gtgtgactga cggaaactcc cgactgtcgg catcgaccag tgccttcacc
     6061 acgatccagc atttttcggc cccttccatt gaaagggcat aataaaccgc gtaatgaccg
     6121 ttaagtgtcc gctcgtcgtt gccaaacagg actggaagcc agccatcatg ttgataataa
     6181 agataatgta caacgtcagg cagcagattg attttcaccg taatggtgac ctgttcaggc
     6241 gtttgtcgct cttcatccag caccgcgccg gggaactgcg ttttcagggc ggcgagaatc
     6301 gcttcgccac gatttgacga tgaattaacg ttcactgtgt ttctccgaaa cagatgcggt
     6361 gcctgaaggc aaaaaatcat gccaggtgct acgttgtgca ggcaggtcgt gcgtccctga
     6421 gaggactata gtggaagcgc ccgccaggat cctgatgaca ggttgtggaa tatgcgttcc
     6481 catcgccagc atcatgacca gcagaatcac cattggcgag gtggtcaacc agccgagatc
     6541 accccggtta acggcctgcg gcggtttcgc cattaacacc cgcgcagcca tccgtaccag
     6601 gcccgccagc accagcgtta acagcaataa cagcaggacg ataatcagca ggtgattacg
     6661 tgccagtcct gcggtaacgg tcataaattc gctaagaaaa atgttgaagg gcggcatccc
     6721 tgccagcgcc agcgccagcg caccgccgcc aaacagcacg gcggtaaatg gcatgatttt
     6781 gagcatccca cagacgacgt tgagatcgcg cgtgccgtac ttgagcagta cattgccgga
     6841 accgcagaac agcagcgttt ttgccagact gtggtttaag gtgtgcagca gcgcggcaaa
     6901 aattcccagc ggcccgccaa tgcctagcgc caccgcgacc agccccatgt tctccacgct
     6961 ggagtacgcc agcagacgct taatgtcccg ctgtaccaga atgaaaaatg ccgccacggc
     7021 aaccgacaac atgccgaaga tgagcaacaa ccggttgggg aaatcgctgc cgatggcctg
     7081 gcaaataatg atgtaatagc gaatcagcac caacagcgcg cagttcagca atacggcgga
     7141 gagcagggcg ctgaccggac tcggcgcttc actgtgagca tccggcagcc aggcgtgcat
     7201 gggaaatagc ccggttttgg taccaaagcc aattagcaca aacacaaagg ccaacagcat
     7261 taatgttggg tcaagcaagg acgattgctt aagaacctcg ctccagaata tcgccatttc
     7321 tgcctgcggc ataacgctgg cggcgttggc gtataccagc acggtaccga acagaccaaa
     7381 agcgacacca acagtacaaa taatgatgta cttccatgct gattccagcg atgaacgctg
     7441 accgtaaatg cctaccagaa acgccgagct taaggtggtg gcttcgatcg ccgcccacat
     7501 cacaatcagg ttattgctgg taacaaccag cagcatggtg aacaaaaaca gatggaagaa
     7561 accgtagtaa tcgcacagtg taacgggtga aagctcgccg tgtgccactt catgacgcat
     7621 gtagccaatc gagtaaacac cggtgagaaa gccaatcaca ccaagaatgg cgaggaacaa
     7681 accgcccaga ccatcaatat gaagccacag tcccgcagcg aatatttctc ctgcatcagc
     7741 ggcagtttgg accacccaga gtgctagaat cagcagcagt gtgatcccta agctatgtaa
     7801 tacagtcacc gtacaagtcg cagagagccc ccgtttccgg caggcaaaac agagcagcga
     7861 aaaaagcagc ggcgtgagca ggagtaaagc gaacatcaca gaataactca tctcattatc
     7921 ccttcagcgc ggtcaagttg ttcacgtcca gcgtgccgtg ggtacgccat atttttcttg
     7981 ccagtaacac catcacaatg acggcgaaga tggcgtcggt agcgatacct atttccacca
     8041 gttccggtgc tcgccaggca agaagcgcca gcaccagatg ggagccgttt tccatcaggc
     8101 agtaaccaaa aatttgccgc aggatattgc gttggctgac aatgcacagc aggccaagca
     8161 gaaaatgacc taacgctacc gccagcgccg gtttcagccc ggtagccatc ggtagcttca
     8221 cgggctgaac gacaaatgcg caaagcagga caattaacgc cgcgagcagt gccatcattg
     8281 ccggaccgaa taacgctttt tccgggatgt tctggggaat atttcgtgca gcgtaagtca
     8341 tgattaacgg taccagcagc actttggtga taaaggcgct ggcggaccag atcagcagtt
     8401 gctctgtggc gaacaggcac gagagagtgg cgaaaataga caccagcacc agcgactggc
     8461 aggcgtaaaa tccgcatgac aggcgatagc ttttgacgct aatcacaaac agcgatgtca
     8521 gcatcatcag tcccgccaga ttatttacga tcatagaacc ggtcattcat catctcctta
     8581 ctgcaaccag gtcgcggcga ttacgctgga aatcagcgac atcacaatca gtacaatcaa
     8641 caccaggcgc attgaccctg gcagcggtgc ggcatcggcg acggcctcgc tcggtttgcc
     8701 cggcacgacg cgaccaaacc agcgaataaa ccaggcgaaa ctggcgaccg attcaatcat
     8761 cagcagaatc atggcgggca gcaggatcca gtactccact gacaacgcaa aaccggcggc
     8821 aaacagcggg aatttactga agaagccgtt gaacggcggc acaccggtaa ttgccagcgc
     8881 tgccacgcag aaacccacgc ctggcaacgg cagggtgtgc aatacgccac gcagacgcgg
     8941 caacaagcgc gtgccgcagc tgtaactcag cgcacctgct acaaggaaaa acaggctttt
     9001 agcgaacgcg tggttgacga tgtaggcgat gctaccctcc agcgccagcc gcgagccgaa
     9061 gatggagagc gacaagccga agaacatcca gccaagttga gtgatggtcg accaggccag
     9121 caaccgcttc atatcctgct gtggcaaata catcagaaag ccataaagaa tggtgaccag
     9181 tgtcatgacc atgccaacgc cgccaatcac atgcgggata ttgccgccgt cgataatggc
     9241 gcgggcaaaa atgtaaacgc ccactttcac catcgatgcg gcgtggagat aggcgctgat
     9301 cggtgttggc gcttccattg cgtccggtag ccacgcttgc atcggtagct gggccgattt
     9361 cccccacgcg gcaaacagga tgccgccata aaccagataa cgtgcgtcgc catgtaactc
     9421 gctcatcgcg ctaagcacaa acgttccggt ctgcaaaaac agcgtggcgg cggcaagata
     9481 caaccccaac gaaccgatat gggtgataag caacgctttt agtgctgaac gctgcgcttt
     9541 atcgctctgg taataactga tcaacgccca ggagcagccg cccgtaattt caaaaaacaa
     9601 caactgaccg agcagcgtcg aggagagtac cagtcccgcc atcgcgccga taaacaccag
     9661 caaaaatgcg taataacgat tcgtgccgtt atgcgggtgt tcgcgatttt tatccgtcag
     9721 ataacccgtg gagtagatcg tgaccagcaa accgagaaac accaccacaa acagaatcag
     9781 cgtactcacg cgatcaatga ccaggccaaa taatgccaca tcccccacgt tgaccaacgt
     9841 caacgtgacg gcaactttat cggcctgata aaacgcggag atcagcgaca acatgcacag
     9901 cgtggtcagc gcggcgaaca aaacccccca ttcggcggcc cgacgttgtg gcgaaaacga
     9961 aacgaccagt gcgccgataa aaggcagcat taacgtcgtc agagcaagat tttccatatt
    10021 ccgtcagtgc tccttacaga ccggttaacc aaaagaccca tgcaagcgca gcaaggctga
    10081 agccaagcca ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg
    10141 cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca
    10201 gcaggcaggc gagagaaagt tcttgcgcgc gcccaaaggg cagaaacagg gccacaaaca
    10261 gtgctgccat cacgacctgt ttcagcccca gcccccattt cgctagcgcc agcccggcac
    10321 cggaatattc ggtcagcggg ccttcctgta attcctgttc tgcttcagca acatcaaagg
    10381 gaatttttcc catctcaatg aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg
    10441 ttagcggcga gttccagccc atagccagcg tattgctgat catctcgata tgcgtggaac
    10501 ctgctatcag cgccaatacc agcagtgaga gaataagcat tggttcgacc agaatgccga
    10561 gcgtcaactc gcgactggca ccgactcccg caaacggact tccggtatcc agcccggaaa
    10621 gagcaaagaa aaaacgaaac agggcaagaa gatagataag ggtgatcaga tcgccgccgc
    10681 ccgcaaaagg ggaaacggta ataaatagtg gtaaggccat cgccagcacc agcatgctgc
    10741 tgattaatac ccacggcatc aggcggaaca tcagacctga agatgtcggc gcaacttcct
    10801 ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca gatccccggc ccgcggcggg
    10861 agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa aagtggggtt agcatcagca
    10921 gaatcacggc ctgtgctaac gcaaaaatga ccagatatcc gtcgcaaaga gtttgtctca
    10981 tggtgatttc cttagacggc aatagcgatt agcagcacaa ccagtgcggc gaccacatac
    11041 aggcaataga gacgaaagtc gccgctttgc agatgctgta tttctttgac cagccgttgg
    11101 gtggcgctca cgatggggcg gatcacccgc tcatcccaga agggttctgt gctctgagcc
    11161 ctggcggtga cgtgcgcaag acctttattt agcctcagcg tagggtcgag ctgttgtcgt
    11221 agacgaaata acgcagagaa gaccacacgc agcggctgca tcacgccatt gcctgacgga
    11281 gccatcgcgt tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct
    11341 gcgcggcgcg aacgacacca cagccagtaa aggcctggca gcaaaggcat cgccagtagt
    11401 aacagcaaca gtaatgaagg agtgacccgt gtatgaaacg tgccgggtac aagtgctatt
    11461 ccgctggcga cagtgacggg aggggtattg gtaaacgcat gggcaatatg cattatcttc
    11521 ggtgccagcc aactggcact aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg
    11581 gcgacgatca tcgtatttgg cacttcctgt gcctcttcag cgtgtgtact gcgcggcgca
    11641 ccacagaaag taataccgta cattttgacg aagcacatta ctgccagccc accagtgact
    11701 gccagcatta ccatagcaat aggacccgca agttgtagcg ctacggcttc cacacgactt
    11761 agtgagaaca gcgactgcca ggtgtaccat tcgctgataa aaccattcag cggaggaatg
    11821 gctgatatcg cgaggcaacc aatcaggcat gctgcggctg tccacggcat ccgtttcgcc
    11881 agtgccccca ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc gtccgcgccg
    11941 agaaatagca gccctttgaa cagcgcatgg ttgagcagat gaaacagtgc gccgagcagt
    12001 ccaaccacgg tgagcagcgg gtcgtgcagt gacagaccgt ccatcgccac accgactgcc
    12061 agcaaaataa tgccgacgtt ttcgacggta ctccaggcca gcagccgttt gatatcctgt
    12121 tccgccagcg catatagcac gcccaggagc gcggagattg cgccgatcgc catcaccaga
    12181 atgccccacc acagaggcaa acccgtttgc gccagcagat ccatcgctac tttcaggatg
    12241 ccgaaaatac cgattttgac cattacgcca gacatcaacg ccgaagcgtg cgatggtgcg
    12301 gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa
    12361 ccgaaaaagg ccagcagaaa caccgccgac gccagccccg gagaaagtga aagcgtgcga
    12421 aactggcaaa atcgaggctg ccgctttcgc gccccatcag caagaaggcg atcataatca
    12481 gcaccgatcc ggcgtgggcg ataaaaaagt agagcatgcc agcgttgatc gacgttttat
    12541 cctgcctggc aatgaccaga aaccaggaag acagcgacat catttcaaac agcacgatga
    12601 accaaaaagc gttgtccatc accagcaggg caaccatcga tgcgatgaaa agattcataa
    12661 agaagcccat cgccgccgcg cctttgccct cgtattcgcg catataagtc aatgaataga
    12721 gcgaacaaac caccaccagc aaggagataa ctagcaccat aaatgccgag aggctatcca
    12781 ttcgcagggt taaatcggca aagtcaaaag gggtggcaaa atgggcgact aaagttacac
    12841 cagcatgtaa ttgcgtaatt ccgctgatga tgccaatcac gccgcccacc agcgatgtga
    12901 tgccggaaag cttaatagcc agtctgtcca gaccgagtaa aaacagcgaa gccagactgg
    12961 caaacagata gaggatcagc gaccaggtta ataattgcag ggcatccatt agtgttgctc
    13021 ctgagtgagg gaaaggggag actccccgcc attggcaaac cagcttgcgg caaggcgctg
    13081 tttttctttc atctgacgtt gcaggctatc gtcggtgatc agccgtaacg cctgattcgg
    13141 gcaagcgcga acgcaggccg gaccttctgg caagaaatca cacaggtcgc atttcaccgc
    13201 gacggtctgg acacccggtt cccagcgcag caacgcatgt tggggcggca cgttttcttc
    13261 gccgtctttg agtgagcctt cagcctgaaa aacatattgc gcatgggcat tcaccggacg
    13321 gcttcctgaa gcgctgattg cgccaaatgg gcagaccacg gcgcaaagct tgcagccaat
    13381 acagaggctt tcgttgagtt ggatcgcatc atccctctga gagatggcat tgaccgggca
    13441 gacctgcagg caaggggctt cctcacagtg atgacacacg acaggggcag tgattgttga
    13501 cgtcttcgcc agggccaggc gcgggtgttg ctgtaaacct tgcgttttat gcacgtccga
    13561 acaggcagcg agacaggtat tacatcctgt acaccacagt ggttcggcca ccacaaagcg
    13621 gttcatgtcg gctccttgaa aatgatatga aacattgcat ttcatattgt ataaaggatg
    13681 ccattctgat taactcaatc tattcaataa gttatgtatt aactggattc tgaacggtac
    13741 aaaaatgatg gtcattaacg ttcagcaaaa aatggattcg acacccgtgt cgcaggggaa
    13801 agcgagaatg tgatcttgat cacgagaagt gaggtatgct ggccgcaagc gcagccagca
    13861 cggaatggag caaagtaatc aggcgtgttc tttcagccag ttcagcacaa cgtcgtggtg
    13921 attgctggtt ttgaaatcgt caaagacatg ttcgattttg ccatcagcgt caatcaggaa
    13981 gctgatgcga tgaataccat cgtaggtttt gcccatgaag gacttttcac cccagacgcc
    14041 gaattgttcg cacacctggt ggtcctcatc agacaggagc gtaaagttaa gcagctcttt
    14101 ttccgcaaaa cgggagagtt tttcgggttt atcggtgctg atacccagca catcaacgcc
    14161 cgcttttttc aactcatcca tgttatcgcg taagccgcag gcctgtacgg tacagccggg
    14221 ggtcatggct ttcgggtaga aataaaccag aacacgctgt ccctggaagt cggtcaaatt
    14281 aacttgttct ccgtcttgat ccggcaagct aaatttcggt gcgatatcac cggctttcag
    14341 tggattcatt acttaactcc atcctgttca tcatgttggg aataattgac gacgttaata
    14401 ctgccttgtg cattgagttc tgtacatagg gctttgaacg cttgctcaat atttgctgcg
    14461 tccgcagatg cggggctgtg ggcggttatc tgaatatgca actgcgcagc ccgttcattt
    14521 tcagcaggtt gtgtgcgcga caccagctcc gcaatgttca tatgatgcgc gtcgaaaagt
    14581 gctgtgaagc gttcaattaa atgcggggag tctgcaacat cgacctgaac ccagacagat
    14641 gctggcattg gcggacgcgg acgcgccgtc gtgcgcttca tcacgattaa aagatccagt
    14701 tcggcacctt tcaacggtaa cgttgattca atcagagtga tggcattcca tgaaccggaa
    14761 agcagcatga taaacgtgaa ctcttctccc agcatcgcca ggcgactgtc ttcaatatta
    14821 cagccgcaac tactgacatg acgggtgatg gtgttcacaa ttccagggcg atcggcaccc
    14881 aacgcagtga tcaccagata atgttgcgat gacagtgtca aactggttat tcctttaagg
    14941 ggtgagttgt tcttaaggaa agcataaaaa aatcatgcat acaacaatca gaacggttct
    15001 gtctgcttgc ttttaatgcc ataccaaacg taccattgag acacttgttt gcacagagga
    15061 tggcccatgt tcacgggaag tattgtcgcg attgttactc cgatggatga aaaaggtaat
    15121 gtctgtcggg ctagcttgaa aaaactgatt gattatcatg tcgccagcgg tacttcggcg
    15181 atcgtttctg ttggcaccac tggcgagtcc gctaccttaa atcatgacga acatgctgat
    15241 gtggtgatga tgacgctgga tctggctgat gggcgcattc cggtaattgc cgggaccggc
    15301 gctaacgcta ctgcggaagc cattagcctg acgcagcgct tcaatgacag tggtatcgtc
    15361 ggctgcctga cggtaacccc ttactacaat cgtccgtcgc aagaaggttt gtatcagcat
    15421 ttcaaagcca tcgctgagca tactgacctg ccgcaaattc tgtataatgt gccgtcccgt
    15481 actggctgcg atctgctccc ggaaacggtg ggccgtctgg cgaaagtaaa aaatattatc
    15541 ggaatcaaag aggcaacagg gaacttaacg cgtgtaaacc agatcaaaga gctggtttca
    15601 gatgattttg ttctgctgag cggcgatgat gcgagcgcgc tggacttcat gcaattgggc
    15661 ggtcatggag ttatttccgt tacggctaac gtcgcagcgc gtgatatggc ccagatgtgc
    15721 aaactggcag cagaagggca ttttgccgag gcacgcgtta ttaatcagcg tctgatgcca
    15781 ttacacaaca aactatttgt cgaacccaat ccaatcccgg tgaaatgggc atgtaaggaa
    15841 ctgggtcttg tggcgaccga tacgctgcgc ctgccaatga caccaatcac cgacagtggt
    15901 cgtgagacgg tcagagcggc gcttaagcat gccggtttgc tgtaaagttt agggagattt
    15961 gatggcttac tctgttcaaa agtcgcgcct ggcaaaggtt gcgggtgttt cgcttgtttt
    16021 attactcgct gcctgtagtt ctgactcacg ctataagcgt caggtcagtg gtgatgaagc
    16081 ctacctggaa gcggcaccgc ttgcggagct tcatgccccg gctggaatga ttttgccggt
    16141 gacctccggt gattatgcaa tcccggtgac caacggtagt ggtgctgtcg gtaaggcgct
    16201 ggacattcgt ccaccagccc agccgttggc actggtttct ggcgcgcgta cccagttcac
    16261 gggcgatacc gcttcattgc tggtggaaaa tggtcgtggc aatactctgt ggccgcaggt
    16321 ggttagcgtg ctgcaggcga aaaactacac catcacccaa cgtgatgatg ctggtcagac
    16381 actgaccacc gattgggtac aatggaaccg tctggacgaa gacgagcagt atcgtggtcg
    16441 ttatcaaatc tctgttaagc cgcagggtta tcagcaggcg gttacggtta aactgctgaa
    16501 cctggaacag gcgggcaaac cggttgcaga cgcggcttcc atgcagcgtt acagcacgga
    16561 gatgatgaac gttatttccg ccggtctgga taaatctgcc actgacgccg cgaacgctgc
    16621 gcaaaatcgt gcctccacca ctatggacgt acaaagtgca gctgatgaca ccggtttacc
    16681 aatgctggtc gtacgcgggc cgttcaatgt ggtttggcaa cgtctgccag cggcgctgga
    16741 aaaagtgggc atgaaagtga ccgacagcac ccgttcgcag ggcaacatgg ccgtaactta
    16801 taagccgctg tctgacagcg actggcagga actgggcgcg agcgatccag gcctggcatc
    16861 cggtgactat aaactgcagg ttggcgattt agataaccgc agcagcctac agttcatcga
    16921 tccgaaaggt catactctga ctcagagtca gaacgacgcg ctggtagctg tcttccaggc
    16981 tgcgtttagc aagtaaaaat acagggctgg aatcatccgg cccttttttc tgatatgata
    17041 cgcaaacgtg tgcgtcggca ggaaaacgcg attttagcgg taattcgcac gaaatttgtt
    17101 tgtcggacgt agttcggata aggcgttcac gccgcatccg acaaaacatc cggcacacca
    17161 gacagcaaaa gattttaaaa cgttaattca cacccaggag tgataaagat gcaaaagcaa
    17221 gctgagttgt atcgtggtaa agcgaaaacc gtatacagca cggaaaaccc ggacctgttg
    17281 gtgctcgaat tccgcaatga tacgtcagca ggggatggcg cgcgcattga gcagtttgat
    17341 cgcaaaggta tggtgaacaa caagttcaac tacttcatta tgagcaaact ggctgaagcg
    17401 ggtatcccga ctcaaatgga gcgtctgctc tccgataccg aatgtctggt gaaaaagctg
    17461 gatatggtgc cggttgagtg tgtcgtgcgt aaccgtgctg ctggctctct agtgaaacgt
    17521 cttggaatcg aagaaggtat tgagctgaac ccgccgctgt tcgatctgtt cctgaaaaac
    17581 gatgccatgc acgatccgat ggtcaacgaa tcttactgcg aaacctttgg ctgggtgagc
    17641 aaagagaacc tggcgcgtat gaaagagctg acctacaaag cgaacgacgt gctgaaaaaa
    17701 ctgttcgatg atgctggtct gattctggtc gacttcaagc tggagtttgg tctgtacaaa
    17761 ggtgaagtgg tactgggcga tgagttctcc ccggacggca gccgcctgtg ggacaaagaa
    17821 acactggaga aaatggacaa agaccgtttc cgccagagcc tcggtggcct gatcgaagcc
    17881 tatgaagccg tcgcccgccg cctgggtgta cagctggact gatttttctg ttcatcatct
    17941 tgccgtgcag ctggcacggc aagacaaccg ctcttggaat atatgcattg aacgctcatt
    18001 cctccgccat ttcatcccgc tttcctgtgg taatcttcct ctgaaccacc tacgatcatt
    18061 tctataatga atatattgtt gaggtatcta tgcgttggca agggcgacgt gaaagtgaca
    18121 atgttgaaga caggcgcaac agctctggcg gtccgtcaat gggcggtccc ggtttccgtc
    18181 tgccgagcgg taaaggcggg ctgattttac tgatagtcgt gctggttgca ggctactatg
    18241 gcgttgattt aaccgggttg atgaccgggc agccggtttc tcaacaacaa tcaacgcggt
    18301 caattagccc taatgaagac gaagccgcaa aattcacctc ggtgattcag gcaaccacgg
    18361 aagacacctg gggacaacag ttcgaaaaga tgggtaagac ctatcagcaa ccgaagctgg
    18421 tcatgtaccg tggaatgacg cgcaccggct gcggggcggg ccagtccatt atggggccgt
    18481 tctattgccc ggcggatggc acagtttata tcgatctctc cttctatgat gacatgaaag
    18541 acaaacttgg cgcggatggc gattttgccc aggggtacgt tatcgcccat gaagtcggtc
    18601 atcatgtgca gaaactgtta ggcatcgagc cgaaagttcg tcaactgcaa caaaacgcga
    18661 cgcaggcgga agtaaaccgc ttatctgtgc gtatggaact ccaggccgac tgttttgccg
    18721 gtgtctgggg gcatagtatg cagcagcaag gcgttctgga aactggcgat ctggaagagg
    18781 cgctgaatgc ggcgcaggcc atcggcgatg accgtttaca acagcaaagt caggggcgag
    18841 tagtaccaga cagtttcaca catggcactt ctcagcaacg ctacagctgg tttaaacgtg
    18901 gtttcgacag cggcgatccg gcacaatgca atacttttgg taaaagcatt taatttccgg
    18961 ggcagggatg gctgaactga ctgcgcttta cacattaaca gcgcaaatga aacgtgaagg
    19021 gatctgccgc ttgctggtgt tgagcgggga agagggttgg tgttttgatc atgctcttaa
    19081 gttgcgtgat gccttacctg gcgactggct gtggatttcg ccgcagctag atgctgaaaa
    19141 ccactgttca ccctcggcgc tacaaacttt acttgggcgc gagttccggc atgcggtatt
    19201 cgacgcccgc cacggctttg atgccgctgc ctttgcagca cttagcggaa cgttgaaagc
    19261 gggaagctgg ctggttttgt tactccctgt atgggaagag tgggaaaacc aacctgatgc
    19321 cgactcgctg cgctggagtg attgccctga ccctattgcg acgccgcatt ttgtccagca
    19381 tctcaaacgc gtacttacgg cggataacga cgctatcctc tggcggcaaa accagccgtt
    19441 ctcgttggcg cattttactc cccgtactga ctggcacccc gcgaccggcg caccacaacc
    19501 agaacaacag caactcttac agcagctact gaccatgccg ccgggcgtgg cagcggtaac
    19561 ggctgcgcgt gggcgcggta agtcggcgtt ggcagggcaa ctcatttctc gtattgcggg
    19621 cagtgcgatt gtcaccgcgc ccgcaaaagc ggcaacggat gtactggcac aatttgcggg
    19681 cgagaagttt cgctttattg caccggatgc cttgttagcc agcgatgagc aagccgactg
    19741 gctggtggtc gacgaagccg cagccatacc tgcgccgttg ttgcatcaac tggtatcgcg
    19801 ttttcctcga acgttgttaa ccactacggt gcagggctac gaaggtaccg gacgtggttt
    19861 tttgctgaaa ttttgcgctc gctttccgca tttacaccgt tttgaactgc aacagccgat
    19921 ccgctgggca cagggatgcc cgctggaaaa aatggttagt gaggcactgg tttttaacga
    19981 tgaaaacttc acccatacac cacaaggcaa tatcgtcatt tccgcatttg aacaaacgtt
    20041 atggcgaagc gagccagaaa cgccgttaaa ggtttatcag ttattgtctg gtgcgcacta
    20101 ccggacctcg ccgctggatt tacgccggat gatggatgca ccagggcaac attttttaca
    20161 ggcggctggc gaaaacgaga ttgccggggc gttgtggctg gtggatgagg gcggattatc
    20221 tcaagaactc agtcaggcgg tatgggcagg ttttcgtcgc ccgcggggta atctggtggc
    20281 ccagtcgctg gcggcgcacg gcagcaatcc actggcggcg acattgcgcg gaaggcgggt
    20341 tagccggata gcagttcatc cggctcgtca gcgggaaggc acagggcgac aacttattgc
    20401 tggagctttg caatatacgc atgacctcga ctatctttcg gtgagttttg gttacactgg
    20461 ggagttatgg cgtttctggc aacgctgcgg ttttgtgctg gtgcggatgg gtaatcatcg
    20521 ggaagccagc agcggttgct atacggcgat ggcgctgtta ccgatgagtg atgcgggtaa
    20581 acagctggct gaacgtgagc attaccgttt acgtcgcgat gcgcaagctc tcgcgcagtg
    20641 gaatggcgaa atgcttcctg ttgatccact aaacgatgcc atcctttctg acgacgactg
    20701 gcttgaactg gccggttttg ctttcgctca tcgtccgcta ttaacatcgt taggttgctt
    20761 attgcgtctg ctacaaacca gtgaactggc attaccggcg ctgcgtgggc gtttacagaa
    20821 aaacgccagt gacgcgcagt tatgtaccac acttaaactt tcaggccgca agatgttact
    20881 ggttcgccag cgggaagagg ccgcgcaggc gctgttcgca ctaaatgagg ttcgcactga
    20941 gcgtctgcgc gatcgcataa cgcaatggca attttttcac tgactccttc aattttctcc
    21001 catggtcatt gtgagtcagc ggcgtaaaat taatgacatc agttaaggag ttcgccatga
    21061 aacatgacca ttttgttgtt caaagcccgg ataaacctgc acaacagttg ttactgcttt
    21121 tccacggtgt cggggataac ccggtggcaa tgggggaaat aggcagctgg tttgcaccgc
    21181 tgtttcctga tgcgctggtg gtgagtgtcg gtggcgcgga gccgagcggt aatccggcgg
    21241 ggcgtcagtg gttttcagtg cagggtatta cggaagataa tcgccaggcg cgtgtggatg
    21301 ccatcatgcc gacgtttatt gaaacggtac gctactggca gaaacagagc ggagtagggg
    21361 ctaacgccac ggcgctaatt ggtttctcac aaggcgcgat tatggcgctg gagagcatta
    21421 aagccgaacc gggccttgct tcgcgcgtca ttgcctttaa cggacgctat gcgagcctgc
    21481 cggagaccgc gtctacagcc acgacaattc acttgattca tggtggtgaa gatccggtta
    21541 tcgatctggc gcatgcagtt gcggcgcagg aagcgttaat cagtgccggt ggtgacgtga
    21601 cgctggatat cgtggaggat ttaggccatg cgatcgacaa tcgcagtatg caatttgccc
    21661 tggatcactt gcgttatacc attccgaaac attatttcga ccaagcgcta agtggcggta
    21721 agcctggcga tgatgatgtg attgaaatga tgtaaaagac agattacctg gcctgataag
    21781 acgcgtgagc gtcgcatcag ggatcgttca ccattgccgg atgcggcgtt aacgccttat
    21841 ccggcctacg gttgagcact cagcttactt cttcggttga tcctttttcg gccagtcgtc
    21901 gtcatcgtcc cacttatcgt tgaaatcacg atgcggggga agctccggct tgttggcgag
    21961 aaatttctta tggtcgacgc gcttgagatc tttaatcacg ttcagcagga cgcctaccaa
    22021 aaagacaatc accagaatcc accaatattt agccagccag tccatgctta tttcctcttg
    22081 cagaaccact catcacgcg
//
LOCUS       DAZSNM010000049        21993 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZSNM010000049 DAZSNM010000000
VERSION     DAZSNM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21993
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.049"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            141..2942
                     /gene="sucA"
                     /locus_tag="ACHW42_001580"
     CDS             141..2942
                     /gene="sucA"
                     /locus_tag="ACHW42_001580"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR8943448.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            2957..4174
                     /gene="odhB"
                     /locus_tag="ACHW42_001581"
     CDS             2957..4174
                     /gene="odhB"
                     /locus_tag="ACHW42_001581"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR8943449.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            4268..5434
                     /gene="sucC"
                     /locus_tag="ACHW42_001582"
     CDS             4268..5434
                     /gene="sucC"
                     /locus_tag="ACHW42_001582"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR8943450.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            5434..6303
                     /gene="sucD"
                     /locus_tag="ACHW42_001583"
     CDS             5434..6303
                     /gene="sucD"
                     /locus_tag="ACHW42_001583"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR8943451.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(6406..7128)
                     /gene="mngR"
                     /locus_tag="ACHW42_001584"
     CDS             complement(6406..7128)
                     /gene="mngR"
                     /locus_tag="ACHW42_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR8943452.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            7298..9214
                     /gene="mngA"
                     /locus_tag="ACHW42_001585"
     CDS             7298..9214
                     /gene="mngA"
                     /locus_tag="ACHW42_001585"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR8943453.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            9232..11865
                     /gene="mngB"
                     /locus_tag="ACHW42_001586"
     CDS             9232..11865
                     /gene="mngB"
                     /locus_tag="ACHW42_001586"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR8943454.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            12714..14282
                     /gene="cydA"
                     /locus_tag="ACHW42_001587"
     CDS             12714..14282
                     /gene="cydA"
                     /locus_tag="ACHW42_001587"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR8943455.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            14298..15437
                     /gene="cydB"
                     /locus_tag="ACHW42_001588"
     CDS             14298..15437
                     /gene="cydB"
                     /locus_tag="ACHW42_001588"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR8943456.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            15452..15565
                     /gene="cydX"
                     /locus_tag="ACHW42_001589"
     CDS             15452..15565
                     /gene="cydX"
                     /locus_tag="ACHW42_001589"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR8943457.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            15565..15858
                     /gene="ybgE"
                     /locus_tag="ACHW42_001590"
     CDS             15565..15858
                     /gene="ybgE"
                     /locus_tag="ACHW42_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR8943458.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            16008..16412
                     /gene="ybgC"
                     /locus_tag="ACHW42_001591"
     CDS             16008..16412
                     /gene="ybgC"
                     /locus_tag="ACHW42_001591"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR8943459.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            16409..17101
                     /gene="tolQ"
                     /locus_tag="ACHW42_001592"
     CDS             16409..17101
                     /gene="tolQ"
                     /locus_tag="ACHW42_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR8943460.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            17105..17533
                     /gene="tolR"
                     /locus_tag="ACHW42_001593"
     CDS             17105..17533
                     /gene="tolR"
                     /locus_tag="ACHW42_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR8943461.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            17598..18839
                     /gene="tolA"
                     /locus_tag="ACHW42_001594"
     CDS             17598..18839
                     /gene="tolA"
                     /locus_tag="ACHW42_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR8943462.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            18972..20264
                     /gene="tolB"
                     /locus_tag="ACHW42_001595"
     CDS             18972..20264
                     /gene="tolB"
                     /locus_tag="ACHW42_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR8943463.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            20299..20820
                     /gene="pal"
                     /locus_tag="ACHW42_001596"
     CDS             20299..20820
                     /gene="pal"
                     /locus_tag="ACHW42_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR8943464.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            20830..21621
                     /gene="cpoB"
                     /locus_tag="ACHW42_001597"
     CDS             20830..21621
                     /gene="cpoB"
                     /locus_tag="ACHW42_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR8943465.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            21786..21861
                     /locus_tag="ACHW42_001598"
     tRNA            21786..21861
                     /locus_tag="ACHW42_001598"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21819..21821,aa:Lys,seq:ttt)
BASE COUNT         5408 a         5357 c         5975 g         5253 t
ORIGIN      
        1 gacactaaga cagtttttaa aggttccttc gcgagccact acgtagacaa gagctcgcaa
       61 gtgaaccccg gcacgcacat cactgtgcgt ggtagtatcc acggcgaagt aagcataaaa
      121 aagatgctta agggatcacg atgcagaaca gcgctttgaa agcctggttg gactcttctt
      181 acctctctgg cgcaaaccag agctggatag aacagctcta tgaagacttc ttaaccgatc
      241 ctgactcggt tgacgctaac tggcgttcga cgttccagca gttacctggt acgggagtca
      301 aaccggatca attccactct caaacgcgtg aatatttccg ccgcctggcg aaagacgctt
      361 cacgttactc ttcaacgatc tccgaccctg acaccaatgt gaagcaggtt aaagtcctgc
      421 agctcattaa cgcataccgc ttccgtggtc accagcatgc gaatctagat ccgctgggac
      481 tgtggcagca agataaagtg gccgatctgg atccgtcttt ccacgatctg accgaagcag
      541 acttccagga aaccttcaac gtcggttcat ttgccagcgg caaagaaacc atgaaactcg
      601 gcgagcttat ttccgccctc aagcaaacct actgcggccc gattggtgcc gagtatatgc
      661 acatcaccag caccgaagaa aaacgctgga tccaacagcg tatcgagtct ggtcgcgcga
      721 ctttcaatag cgaagagaaa aaacgcttct taagcgaact gaccgccgct gaaggccttg
      781 agcgttacct cggcgcaaaa ttccctggcg caaaacgctt ctcgctggaa ggcggtgacg
      841 cgttaatccc gatgcttaaa gagatgatcc gccacgctgg caacagcggc acccgcgaag
      901 tggttctcgg gatggcgcac cgtggtcgtc tgaacgtgct ggtgaacgtg ctgggtaaaa
      961 aaccgcaaga cttgttcgac gagttcgccg gtaaacataa agaacacctc ggcacaggcg
     1021 acgtgaaata ccacatgggc ttctcgtctg acttccagac cgatggcggc ctggtgcacc
     1081 tggcgctggc gtttaacccg tctcaccttg agattgtaag cccggtcgtt atcggctctg
     1141 ttcgtgcccg tctggacaga cttgatgagc cgaacagcaa caaagtgctg ccaatcacca
     1201 tccacggtga cgccgcagtg accgggcagg gcgtggttca ggaaactctg aacatgtcga
     1261 aagcgcgtgg ttatgaagtt ggcggtacgg tacgtatcgt tatcaacaac caggttggtt
     1321 tcaccacctc taacccgctg gatgcccgtt ctacaccgta ctgtactgat atcggtaaga
     1381 tggttcaggc accgattttc cacgttaacg cggatgatcc ggaagccgtt gcctttgtga
     1441 cccgtctggc gctcgatttc cgtaacacct ttaaacgtga tgtcttcatc gacctggtat
     1501 gctaccgccg tcacggccac aacgaagccg acgagccgag cgcaacccag ccactgatgt
     1561 atcagaaaat caaaaaacat ccgacaccgc gcaaaatcta cgctgacaag ctggagcagg
     1621 aaaaagtggc gacgctggaa gatgccaccg agatggttaa cctgtaccgc gatgcgctgg
     1681 atgctggcga ttgcgtagtg gcagagtggc gtccgatgaa catgcactct ttcacctggt
     1741 cgccgtacct caaccacgaa tgggacgaag agtacccgaa caaagttgag atgaagcgcc
     1801 tgcaggaact ggctaaacgt atcagtacgg taccagaagc agttgaaatg cagtctcgcg
     1861 ttgccaagat ttatggcgat cgccaggcga tggcagccgg tgagaaactg ttcgactggg
     1921 gcggtgcgga aaacctcgct tacgccacgc tggttgatga aggcattccg gttcgcctgt
     1981 cgggtgaaga ctccggtcgc ggtaccttct tccaccgcca cgcggtgatc cacaaccagt
     2041 ctaacggttc cacttacacg ccgctgcaac acatccataa cggtcagggc gcgttccgtg
     2101 tctgggactc cgtactgtca gaagaagccg tgctggcgtt tgaatatggt tatgccaccg
     2161 cagaaccacg caccctgacc atctgggaag cgcagttcgg tgacttcgcc aacggtgcgc
     2221 aggtggttat cgaccagttc atctcctctg gcgagcagaa atggggccgg atgtgtggtc
     2281 tggtgatgtt gctgccgcac ggttacgaag ggcaggggcc ggagcactcc tccgcgcgtc
     2341 tggaacgtta tctgcaactt tgtgctgagc aaaacatgca ggtttgcgta ccgtctaccc
     2401 cggcacaggt ttaccacatg ctgcgtcgtc aggcgctgcg cgggatgcgt cgtccgctgg
     2461 tcgtgatgtc gccgaaatcc ctgctgcgtc atccgctggc ggtttccagc ctcgaagaac
     2521 tggcgaacgg caccttcctg ccagccatcg gtgaaatcga cgagcttgat ccgaagggcg
     2581 tgaagcgcgt agtgatgtgt tctggtaagg tttattacga cctgctggaa cagcgtcgta
     2641 agaacaatca acacgatgtc gccattgtgc gtatcgagca actctacccg ttcccgcata
     2701 aagcgatgca ggaagtgttg cagcagtttg ctcacgtcaa ggattttgtc tggtgccagg
     2761 aagagccgct caaccagggc gcatggtact gcagccagca tcatttccgt gaagtgattc
     2821 cgtttggggc ttctctgcgt tatgcaggcc gcccggcctc cgcctctccg gcggtagggt
     2881 atatgtccgt tcaccagaaa cagcaacaag atctggttaa tgacgcgctg aacgtcgaat
     2941 aaataaagga tacacaatga gtagcgtaga tattctggtc cctgacctgc ctgaatccgt
     3001 agccgatgcc accgtcgcaa cctggcataa aaaacccggc gacgcagtcg tacgtgatga
     3061 agtgctggta gaaatcgaaa ctgacaaagt ggtactggaa gtaccggcat cagcagacgg
     3121 cattctggat gcggttctgg aagatgaagg tacaacggta acgtctcgtc agatccttgg
     3181 tcgcctgcgt gaaggcaaca gcgccggtaa agaaaccagc gccaaatctg aagagaaagc
     3241 gtccactccg gcgcaacgcc agcaggcgtc tctggaagag caaaacaacg atgcgttaag
     3301 cccggcgatc cgtcgcctgc tggctgaaca caatctcgac gccagcgcca ttaaaggcac
     3361 cggtgtgggt ggtcgtctga ctcgtgaaga tgtggaaaaa catctggcga aagccccggc
     3421 gaaagagtct gctccggcag cggctgctcc ggcggcgcaa ccggctctgg ctgcacgtag
     3481 tgaaaaacgt gtcccgatga ctcgcctgcg taagcgtgtg gcagagcgtc tgctggaagc
     3541 gaaaaactcc accgccatgc tgaccacgtt caacgaagtc aacatgaagc cgattatgga
     3601 tctgcgtaag cagtacggtg aagcgtttga aaaacgccac ggcatccgtc tgggctttat
     3661 gtccttctac gtgaaagcgg tggttgaagc cctgaaacgt tacccggaag tgaacgcgtc
     3721 tatcgacggc gatgacgtgg tttaccacaa ctatttcgac gtcagcatgg cggtttctac
     3781 gccgcgcggc ctggtgacgc cggttctgcg tgatgtcgat accctcggca tggcagacat
     3841 cgagaagaaa atcaaagagc tggcagtcaa aggccgtgat ggcaagctga cggttgaaga
     3901 tctgaccggt ggtaacttca ccatcaccaa cggtggtgtg ttcggttccc tgatgtctac
     3961 gccgatcatc aacccgccgc agagcgcaat tctgggtatg cacgctatca aagatcgtcc
     4021 gatggcggtg aatggtcagg ttgagatcct gccgatgatg tacctggcgc tgtcctacga
     4081 tcaccgtctg atcgatggtc gcgaatccgt gggcttcctg gtaacgatca aagagttact
     4141 ggaagagccg acgcgtctgc tgctggacgt gtagtagttt aagcttcacc tgcactgtag
     4201 accgacgtgg atagggtgtc ggtctacggt ttaaaagata acgattactg aaggatggac
     4261 agaacacatg aacttacatg aatatcaggc aaaacaactg tttgcccgct atggcttacc
     4321 agcaccggtg ggttatgcct gtactactcc gcgcgaagca gaagaagccg cttcaaaaat
     4381 cggtgccggt ccgtgggtag tgaaatgtca ggttcacgct ggtggccgcg gtaaagcggg
     4441 cggtgtgaaa gttgtaaaca gcaaagaaga catccgtgct tttgcagaaa actggctggg
     4501 caagcgtctg gtaacgtatc aaacagatgc caatggccaa ccggttaacc agattctggt
     4561 tgaagcggcg accgatatcg ctaaagagct gtatctcggt gccgttgtag accgtagttc
     4621 ccgtcgcgtg gtctttatgg cctccaccga aggcggcgtg gaaatcgaaa aagtggcgga
     4681 agaaactccg cacctgatcc ataaagttgc gcttgatccg ctgactggcc cgatgccgta
     4741 tcagggacgc gagctggcgt tcaaactggg tctggaaggt aaactggttc agcagttcac
     4801 caaaatcttc atgggcctgg cgaccatttt cctggagcgc gacctggcgc tgatcgaaat
     4861 caacccgctg gtcatcacca aacagggcga tctgatttgc ctcgacggca aactgggcgc
     4921 tgatggcaac gccctgttcc gccagcctga tctgcgcgaa atgcgtgacc agtcgcagga
     4981 agatccgcgt gaagcacagg ctgcacagtg ggaactgaac tacgttgcgc tggacggtaa
     5041 catcggttgt atggttaacg gcgcaggtct ggcgatgggt acgatggaca tagttaaact
     5101 gcacggcggc gaaccggcta acttcctgga cgttggcggc ggcgcaacca aagaacgtgt
     5161 aaccgaagcg ttcaaaatca tcctctctga cgacaaagtg aaagccgttc tggttaacat
     5221 cttcggcggt atcgttcgtt gcgacctgat cgctgacggt atcatcggcg cggtagcgga
     5281 agtgggtgtt aacgtaccgg tcgtggtacg tctggaaggt aacaacgccg aactcggcgc
     5341 gaagaaactg gctgacagcg gcctgaatat tattgcagca aaaggtctga cggatgcagc
     5401 tcagcaggtt gttgccgcag tggaggggaa ataatgtcca ttttaattga taaaaacacc
     5461 aaggttatct gccagggctt taccggtagc caggggactt tccactcaga acaggccatt
     5521 gcatacggca ctaaaatggt tggtggcgta actccaggta aaggcggcac tacccacctc
     5581 ggcctgccgg tgttcaacac cgtgcgtgaa gctgttgctg ctactggcgc ttccgcgtct
     5641 gttatttacg taccagcacc gttctgcaaa gactccattc tggaagctat cgatgcaggc
     5701 atcaaactga ttatcaccat cactgaaggc atcccgacgc tggatatgct gaccgtgaaa
     5761 gtgaaactgg acgaagcagg cgttcgtatg atcggcccga actgcccagg cgttatcacc
     5821 ccgggtgaat gcaaaattgg tatccagccg ggtcacattc acaaaccggg taaagtgggt
     5881 atcgtttccc gttccggtac actgacctat gaagcggtta aacagaccac ggattacggt
     5941 ttcggtcagt cgacctgtgt cggtatcggc ggtgacccga tccctggctc taactttatc
     6001 gacattctcg aaatgttcga aaaagatccg cagaccgaag cgatcgtgat gatcggtgag
     6061 atcggcggta gcgctgaaga agaagcggca gcgtacatca aagagcatgt taccaagcca
     6121 gttgtgggtt acatcgctgg tgtgactgcg ccgaaaggca aacgtatggg ccacgcgggt
     6181 gcgatcattg ccggtgggaa agggactgcg gatgagaaat tcgctgctct ggaagccgca
     6241 ggcgtgaaaa ccgttcgcag cctggcggat atcggtgaag cactgaaaac tgttctgaaa
     6301 taaatatctg taataagaaa tagccctcgc cgcttccctc tacaggaatg gcgaagggct
     6361 gtcggtttcg acatggttgg ccatcgtatg atggcctttt tgtgcttatc gcgatgattt
     6421 tcgctgcgct atcagggtaa atttatagtc atcggtatta aaagcgttgc ggctatattc
     6481 aaacacccga ccatcaacta aatatccacg cgatactttt tcaagaatcg gctttgtctg
     6541 gctgatatta agcagacggc tcatctcttc ggttggcatc agaggaatga tttcctgttc
     6601 gctacgatcg ataaccatct tcttcacttc ttcaataaag tgatatttcg aattttccat
     6661 gacctgccag gtgagatccg ggaacaacgc aagcggcatc caggtttctt ccagcgccat
     6721 tggcttttgc ttgcgatagc gcacgcgctt cacatgccaa acacgatcct gcacggtgat
     6781 ttgtagctgt tgctgaagaa aatcgtcagc cggaatcact tcaaatatca gaacttcact
     6841 gtgtgtatcg acgtgacggt ccgacagttt ttcatcaaaa ctggttaact gaaaaatatc
     6901 gtaattgacc cgctcttctt tgacgtaagt cccgctgccc tgaatgcttt cgaggatctg
     6961 ttgctcgact aactggcgca aagcctgacg caccgtaacc cggctgacgc caaactctgt
     7021 ttgtaatgcc gattcggtag gtaacgcatc gccaggttta agctcgccac gcgcaatttg
     7081 ctcacgaatg cgatcagcaa tctgtcggta taagggtttg tgtcccatta tttagcatct
     7141 cattaatacg aatttagcca ttatgcccga taaactcatc ctgtaaacaa tacaaataaa
     7201 atgcaaataa tttcaatcaa gtgaaattga ttacgcaatg gtattgtttt gtcgggcaca
     7261 ctggcgcgac tataaaaacg atcaagtgag gatcatgatg aacctgacga ctctgaccca
     7321 ccgcgatgcg ttgtgtctga atgcgcgctt taccagccgt gaagaggcca ttcacgcgtt
     7381 gactcaacgt cttgctgctc tggggaaaat ttccagtact gagcaatttc tgaaagaagt
     7441 gtatcgccgt gaaagccttg gcccgaccgc cttaagtgaa gggttggctg tgccgcatgg
     7501 caaaactgct gcggtaaaag aagcggcgtt tgcggtcgcc acactcagcg agccgcttca
     7561 gtgggaaggc gtggatggcc cggaagcggt tgatttagtg gtgttgctgg cgattcccct
     7621 caatgaagcg ggtactacgc acatgcaact gctgacagcg ctgaccacgc gcctggcgga
     7681 tgatgagatt cgggcgcgta tacagtcggc gacgacgcct gatgaattgc tctcggcgct
     7741 ggatgacaag ggaggcacgc aaccttctgc ctctttttcc aacgcgccaa ctatcgtttg
     7801 cgtaacggcc tgcccggcgg gtattgctca cacctacatg gctgcggagt atctggaaaa
     7861 agccggacgc aaactcggcg tgaatgtcta cgttgaaaag caaggtgcta acggtattga
     7921 agggcgttta acggcggatc aactcaatag tgcaactgcc tgtatttttg cggctgaagt
     7981 cgccatcaag gagagtgagc gttttaacgg cattcctgcg ctttctgtgc ctgttgccga
     8041 gccgattcgc catgcagaag cgttgatcca acaatcgctt accctcgagc gtggcgatga
     8101 gacgcgtacc ttacagcaag atacgcaacc ggtgaaaagc gtcaaaacgg agctgaaaca
     8161 ggcactgttg agcgggatct cttttgccgt accgttgatt gtcgcggggg ggacggtgct
     8221 ggcggtcgcg gtattactgt cgcaaatctt cgggctacaa gatctgttta atgaagaaaa
     8281 ctcctggctg tggatgtacc gcaaactggg cggcgggctg ctcggaattt tgatggtacc
     8341 ggtgctggca gcctataccg cctattcgct ggcagataaa ccggcgttag cgccaggctt
     8401 tgcggctgga cttgccgcca acatgatcgg ctccgggttt ctcggcgcgg tcgttggcgg
     8461 attgatagcc ggttacttga tacgctggat gaaaaatcac ttgcgtctta gcagtaaatt
     8521 caatggcttc ctgacttttt atctctaccc ggtgctcggt acattgggag cgggcagtct
     8581 gatgctgttt gtggtggggg aacctgtcgc ctggatcaat aactcgctta ccgcctggct
     8641 gaacggtctg tcaggaagta acgcgctgtt gctgggtgcc attctcggtt ttatgtgttc
     8701 ctttgacctt ggagggccag tgaataaagc cgcttatgca ttctgcctgg gcgcaatggc
     8761 gaacggcgtt tacggcccgt atgccatttt cgcctccgtc aaaatggttt cggcatttac
     8821 cgtaaccgct tccacgatgc tcgcaccgcg cctgtttaaa gagtttgaaa ttgagaccgg
     8881 gaaatccacc tggctgttag ggctggcagg tattaccgaa ggggcgatcc cgatggcgat
     8941 tgaagatccg ctgcgggtta ttggttcgtt tgtgctgggc tctatggtaa cgggcgctat
     9001 tgtcggtgcg atgaatatcg gcctttcgac acccggtgct ggcattttct cgctcttttt
     9061 acttcatgat aatggcgcgg gcggtgttat ggcggcaatt ggctggtttg gtgcggcatt
     9121 ggtgggggct gcaatctcga ctgcaattct cctgatctgg cggcgtcacg cggttaagca
     9181 tggcaactat ctgactgatg gcgtaatgcc ataaacaaaa aggaaacgac gatgaaagcg
     9241 gtatctcgcg ttcacatcac cccgcatatg cactgggatc gggagtggta tttcaccacc
     9301 gaagagtcac gcattctgct ggtcaataat atggaagaga tcctgtgccg actggaacag
     9361 gacaacgaat acaaatatta cgtactcgac gggcaaacgg cgatcctcga agattatttc
     9421 gcggtgaaac cggaaaacaa agaccgcttg aagaaactgg tagaagccgg caagttgatt
     9481 atcggcccct ggtataccca gaccgatacc acgattgttt ctgcggaatc catcgtccgt
     9541 aatctgatgt acggaatgcg tgactgcctc gcgtttggcg agccgatgaa aataggttat
     9601 ttacccgatt cctttggcat gtccgggcaa ctgccgcata tctacaatgg atttggcatt
     9661 acctgcacca tgttctggcg tggatgttct gagcgccacg gtacggataa aaccgagttt
     9721 ttgtggcaaa gcagtgacgg tagcgaagtg acggcgcagg tgatgccgtt gggttatgcc
     9781 atcggtaagt acttacctgc ggacgaaaac ggattacgca aacgcctcga cagttatttt
     9841 gacgtgctgg aaaaagcgtc tgtaaccaaa gagattttgc tgccgaatgg gcatgatcag
     9901 atgccattgc agcaaaatat cttcgaagtg atggagaaac tgcgcgaaat ttatcctcaa
     9961 cgaaagtttg tgatgagccg ctttgaagag gtatttgaga agatcgaagc gcagcgagat
    10021 aatctggcaa ccctgaaagg ggaatttatt gatggcaaat atatgcgcgt gcatcgcacc
    10081 atcggttcca cgcgcatgga tatcaaagtt gcccacgcac gtattgaaaa taagattgtt
    10141 aatctgctgg aaccgctggc aacgctggcc aggacgttgg gttttggata ccaccacggc
    10201 ttgctggaga aaatgtggaa agagatctta aaaaatcatg cccacgacag catcggctgc
    10261 tgctgtcgtg acaaagttca tcgcgaaatc gtcgcccgct tcgaactggc tgaagacatg
    10321 gcggataatc tgctgcgttt ctatatgcgt aaaatcgccg acaacatgcc gcagagcgac
    10381 gccgacaaac tcgtcctgtt taacctgatg ccctggccgc gggaagaagt gatcaacacc
    10441 actgtgcggc tgcgcgccag ccagtttaat ctacgagacg atcgcggtca gcctgtaccg
    10501 tattttattc gccatgcccg tgagatcgat ccaggcctaa tcgatcggca aatagttcat
    10561 tacggtaatt acgatccctt tatggagttt gatatacaga tcaaccagat tgtcccttct
    10621 atgggctatc gcacgcttta tatcgaagcg aatcagcctg gcaacgtgat tgcggcaaaa
    10681 agtgacgctg aagggatact ggaaaatgct ttctggcaaa ttgcgctcaa tgaggatgga
    10741 acgctacaac tggtagataa agatagcggt gtgcgctatg accgggtgtt gcaaattgaa
    10801 gaaagctctg atgatggtga tgaatatgac tattcacccg caaaagaaga gtgggtaatt
    10861 acttcggcga atgcgaaacc gcaatgcgag attattcatg aggcctggca gagcagggct
    10921 gttatccgct atgaaatggc ggtgccgcgt aatctgcaac aacgcagcgc aagacaatcc
    10981 actggcagag tagatgtcga gttggttgtc actcttagtc ataacaacag gcgcattgat
    11041 gtggatatca accttgataa ccaggctgac gatcatcgcc ttcgcgtcct gatccctaca
    11101 tcttttaaca ccgatagtgt tctggcagat acccagtttg gttcgctaac gcgccccgtg
    11161 aacgacagtg caatgaacaa ctggcagcaa gaaagctgga aagaagcgcc ggttccggta
    11221 tggaatatgc tcaactatgc agccttacag gaagggcgta acggcatggc tgtctttagc
    11281 gaagggctac gtgaatttga agtcatcggt gaagagaaga aaacctttgc cattacgttg
    11341 ctgcgcggcg tgggcttact ggggaaagaa gatctgcttt taaggcctag gcggccttcg
    11401 ggaattaaaa tgccagtccc ggactcacaa ctacgtggtc tgctttcttg tcgcctaagt
    11461 ttattgagtt ataccggtac gccaaccgcc gctggtgtag ctcagcaggc gcgagcatgg
    11521 ctgactccag tacagtgtta caacaaaatc ccatgggatg cgatgaagct caacaaagcc
    11581 ggattcaacg tgccggaaag ttatagtttg ttgaaaatgc ccccagtggg atgcctgata
    11641 agcgcactta agaaagctga agaccgacaa gaagtgattt tacggctgtt taatccggct
    11701 gaatcagcaa cctgtgatgc gactgttgct ttcagtcgcg aggtgatttc ttgctcagaa
    11761 acgatgatgg atgaacacat taccaccgag gaaaatcaag gttcaaatct atcggggcct
    11821 tttttacccg gccagtcacg gacgttcagt taccggcttg cctgaatagc aatcaaaccg
    11881 aagccacata tgcgcggcca gattgttgac aaagggcgct ttgttcatgc cggatacggc
    11941 atgaacgctt tattcggtct acaaaagcag gcaaattcaa tatattgcag agatgatgta
    12001 gacactggca agcgtagcgc atcaggtaat tttgtgttta tcttcactct caagctacgt
    12061 atatgtggct ttatttttaa caaaataata accacgggtg agttaattat aatataatta
    12121 taagttaatt aaatgttaat atcggcggga gggagattta tgcctttatt aataatcctg
    12181 aaactctgcg tcgtattagc cagtgaccaa aaaaagaatt aaggtcaacc gtgctgtttt
    12241 tgcttcgtct ctttttatct ttaattgcca accgaaacta atttcagtct tataattcac
    12301 acattttaaa cataaatatc actaaagtta ccttgttgaa acatgattaa cataatttgt
    12361 agaaattgat atttatcaat gtataagtct tggaaatggg catcaaaaag agataaattg
    12421 ttctcgatca aattggctga aaggcggcaa tttagctata aattgatcac cgtcgaaaaa
    12481 tgcaaatttg cttcaacaaa aacctgttta ttgtaaggat tttgcggcgt aatatatacg
    12541 tgggatcaat ttgagttttt attaacatgt ttgcaacctt tctttacgcc gtttttgtgt
    12601 gcattcacat ggtatgatga aagtgttcaa acaaatttct attggggcat gcgtgtgacc
    12661 ctttctaacg gggttcactc tcggagtctt catgcgatga gcaaggagtc atgatgttag
    12721 atatagtcga actgtcgcgc ttacagtttg ccttgaccgc gatgtaccac ttcctttttg
    12781 tgccactgac gctcggtatg gcgttcctcc tggccattat ggaaacggtc tacgtcctct
    12841 ccggcaaaca gatttataaa gatatgacca agttctgggg caagttgttt ggtatcaact
    12901 tcgctctggg tgtggctacc ggtctgacca tggagttcca gttcgggact aactggtctt
    12961 actattccca ctatgtaggg gatatcttcg gtgcgccgct ggcaatcgaa ggtctgatgg
    13021 ccttcttcct cgaatccacc tttgtaggtc tgttcttctt cggttgggat cgtctgggta
    13081 aagttcagca tatgtgtgtc acctggctgg tggcgctcgg ttcaaacctg tccgcactgt
    13141 ggattctggt tgcgaacggc tggatgcaaa acccaatcgc gtccgatttc aactttgaaa
    13201 ctatgcgtat ggagatggtg agcttctccg agctggtgct taacccggtt gctcaggtga
    13261 aattcgttca cactgtagcg tctggttatg tgactggcgc gatgttcatc ctcggtatca
    13321 gcgcatggta tatgctgaaa ggtcgtgact tcgccttcgc taaacgctcc tttgctatcg
    13381 ctgccagctt cggtatggct gctgttctgt ctgttattgt tctgggtgat gaatccggct
    13441 acgaaatggg cgacgtgcag aaaaccaaac tggctgctat tgaagccgag tgggaaacgc
    13501 aacctgcgcc tgctgccttt actttgttcg gcattcctga tcaggaagag gagacgaaca
    13561 aatttgcgat tcagatccct tacgcactgg gcatcattgc aacgcgttcc gtggataccc
    13621 cggttatcgg cctgaaagag ctgatggtgc agcatgaaga acgcattcgt aacgggatga
    13681 aggcgtactc tctgctcgaa caactgcgtt ctggttctac cgaccagacg gttcgtgacc
    13741 agttcaatag catgaagaaa gacctcggtt acggtctgct gctgaaacgc tatacgccaa
    13801 acgtggctga tgcgactgaa gcgcagattc aacaggcaac caaagactcc atcccgcgtg
    13861 tagcgccgct gtactttgcg ttccgtatca tggtggcgtg tggcttcctg ctgctggcaa
    13921 tcatcgcgct ctctttctgg agtgtcatcc gcaaccgcat tggcgagaaa aaatggcttc
    13981 tgcgcgccgc gctgtacggt attccgctgc cgtggattgc tgtagaagcg ggctggttcg
    14041 tggctgaata tggccgccaa ccgtgggcta tcggtgaagt gctgccgaca gctgtggcga
    14101 attcgtcact gaccgcaggc gatctcatct tctcaatggt gctgatttgc ggcctgtata
    14161 ccctgttcct ggtggcagaa ttgttcttaa tgttcaagtt tgcacgcctc ggcccaagca
    14221 gcctgaaaac cggtcgctat cactttgagc agtcttccac gactactcag ccggcacgct
    14281 aagacaggag tcgtcaaatg atcgattatg aagtattgcg ttttatctgg tggctgctgg
    14341 ttggcgttct gctgattggt tttgcagtca ctgacggttt cgacatgggg gtgggcatgc
    14401 tcacccgttt cctcggtcgt aacgacaccg agcgtcgaat tatgattaac tccattgcac
    14461 cacactggga cggtaaccag gtttggctga tcaccgcggg cggcgcactc tttgctgcct
    14521 ggccgatggt ctatgccgct gcgttctccg gcttctatgt ggcgatgatc ctcgtgctgg
    14581 cgtctttgtt cttccgtccg gtcggttttg actaccgctc caagattgaa gaaacccgct
    14641 ggcgtaacat gtgggactgg ggcatcttca ttggtagctt cgttccgccg ctggtaattg
    14701 gtgtagcgtt cggtaacctg ttgcagggcg taccgttcaa cgttgatgaa tatctgcgtc
    14761 tgtactacac cggtaacttc ttccagttgc ttaacccgtt cggcctgctg gcaggcgtgg
    14821 tgagcgtagg gatgatcatt actcagggcg caacctatct gcaaatgcgt accgtgggcg
    14881 aactgcacct gcgtacccgt gcaacggctc aggtggctgc gctggtgaca ctggtctgtt
    14941 tcgcactggc tggcgtatgg gtgatgtacg gtatcgatgg ttatgtcgtg aaatcgacaa
    15001 tggaccatta cgcagcctct aacccactga ataaagaagt ggttcgtgaa gctggcgcat
    15061 ggctggttaa cttcaacaac acgccaattc tgtgggctat tccggcactg ggtgtggttc
    15121 tgctgctgct gaccatcctg actgcacgta tggataaagc cgcgtgggcg tttgtgttct
    15181 cctccctgac gctggcctgc atcatcctga cagccggtat cgcaatgttc ccgtttgtga
    15241 tgccgtccag caccatgatg aacgcaagtc tgacaatgtg ggatgcaact tccagccagc
    15301 tgacgcttaa cgtcatgacc tgggttgcgg tggttctggt accgatcatt ctgctctaca
    15361 ccgcctggtg ttactggaaa atgttcggtc gtatcaccaa agaagatatt gaacgtaaca
    15421 cccactctct gtactaagta aggagctaaa aatgtggtat ttcgcatgga ttctgggaac
    15481 gcttcttgcc tgttcgtttg gggtaatcac cgcgctggcg cttgaacacg tcgaatcagg
    15541 caaagccggt caagaagaca tctgatgagt aagattatcg cgaccttgta tgcggtaatg
    15601 gacaagcgcc ccctgcgggc gctttccttc gtgatggcgc ttctgttagc aggatgtatg
    15661 ttttgggacc catctcgttt tgccgcgaag accagtgaac tggaaatctg gcatggttta
    15721 ttgctgatgt gggccgtctg tgctggtgtg attcacggcg tgggctttcg tccgcagaag
    15781 gttctttggc aagggatttt ttgcccattg cttgccgata ttgttctcat tgtcgggctg
    15841 attttcttct tcttttaaat cagaattctc tttaaaaaat tatgggccgc tctaggccca
    15901 taaattttta cgcgccctta acttgccctc attcccaaac ctcaatcgcg cgcgtatagt
    15961 agcagcgttt aaaagctcta acttttgttg cattaccggg atgtaaagtg aatacaacgc
    16021 tgtttcgatg gccggttcgc gtctactatg aagataccga tgccggtggt gtggtgtacc
    16081 acgccagtta cgtcgctttt tatgaaagag cacgcacaga gatgctgcgt catcatcact
    16141 tcagtcagca ggcgctgatg gctgaacgcg ttgcctttgt ggtacgtaaa atgacggtgg
    16201 aatattacgc acctgcgcgg ctcgacgata tgctcgaaat acagactgaa ataacatcaa
    16261 tgcgtggcac ctctttggtt ttcacgcaac gtattgtcaa cgccgagaat actttgctga
    16321 atgaagcaga ggttctggtt gtttgcgttg acccactcaa aatgaagcct cgtgcgcttc
    16381 ccaagtctat tgtcgcggag tttaagcagt gactgacatg aatatccttg atttgttcct
    16441 gaaggctagc cttctggtta aacttatcat gttgattttg attggttttt caatcgcatc
    16501 ctgggccatt attatccagc gaacccgtat tcttaacgca gcggcgcgtg aagccgaagc
    16561 gtttgaagat aaattctggt ctggaatcga actctctcgc ctctatcaag agagtcaggg
    16621 gaaacgggat aatctgactg gttcggaaca aatcttttac agcgggttca aagagtttgt
    16681 gcgcctgcat cgtgccaata gccatgcgcc ggaagccgta gtggaagggg cgtcgcgtgc
    16741 tatgcgtatc tccatgaacc gtgaacttga aaatctggaa acgcacattc cgttcctcgg
    16801 tacggttggc tccatcagcc cgtatattgg tctgtttggt acggtctggg ggatcatgca
    16861 cgcctttatc gccctcgggg cggtaaaaca agcaacactg caaatggttg cgcccggtat
    16921 cgcagaagcg ttgattgcga cggcaattgg tctgttcgcc gcaattccgg cggtaatggc
    16981 ttataaccgt ctcaaccagc gcgtaaacaa actggaactg aattacgaca actttatgga
    17041 agagtttacc gcgattctgc accgccaggc gtttaccgtt agcgagagca acaaggggta
    17101 agccatggcc agagcgcgtg gacgaggtcg tcgcgatctc aagtccgaaa tcaacattgt
    17161 tccgttgctg gacgtactgc tggtgctgtt gctgatcttt atggcgacag cgcccatcat
    17221 cacccagagc gtggaggtcg atctgccaga cgctactgaa tcacaggcgg tgagcagtaa
    17281 cgataatccg ccagtgattg ttgaagtgtc tggtattggt cagtacaccg tggtggttga
    17341 gaaagatcgc ctggagcgtt taccaccaga gcaggtggtg gcggaagtgt ccagccgttt
    17401 caaggccaac ccgaaaacgg tctttctgat cggtggcgca aaagatgtgc cttacgatga
    17461 aataattaaa gcactgaact tgttacatag tgcgggtgtg aaatcggttg gtttaatgac
    17521 gcagcctatc taaacatctg cgtttccctt gcttgaaaga gagcgggtaa caggcgaaca
    17581 gtttttggaa accgagagtg tcaaaggcaa ccgaacaaaa cgacaagctc aagcgggcga
    17641 taattatttc agcagtgctg catgtcatct tatttgcggc gctgatctgg agttcgttcg
    17701 atgagaatat agaagcttca gccggaggcg gcggtggttc gtccatcgac gctgtcatgg
    17761 ttgattcagg tgcggtagtt gagcagtaca aacgcatgca aagccaggaa tcaagcgcga
    17821 agcgttctga tgaacagcgc aagatgaagg aacagcaggc tgctgaagaa ctgcgtgaga
    17881 aacaagcggc tgaacaggaa cgcctgaagc aacttgagaa agagcggtta gcggctcagg
    17941 agcagaaaaa gcaggctgaa gaagccgcaa aacaggccga gttaaagcag aagcaagctg
    18001 aagtggcggc agcgaaagcg gcggcagatg ctaaagctgc ggaagaagca gcgaagaaag
    18061 cggctgcaga cgcgaagaaa aaagcagaag cagaagccgc caaagccgca gccgaagcgc
    18121 agaaaaaagc cgaggtagcc gctgcggcac tgaagaagaa agcggaagcg gcagaagcag
    18181 ctgcagctga agcaagaaag aaagcggcaa ctgaagctgc tgaaaaagcc aaggcagaag
    18241 ctgagaagaa agcggctgct gaaaaggctg cagctgataa gaaagcggca gcagagaaag
    18301 ctgcagccga caaaaaagca gcagaaaaag cggctgctga taaggcagca gctgataaga
    18361 aagcagcggc agaaaaagcc gccgcagaca aaaaagcggc agcggcaaaa gcagcagctg
    18421 aaaaagccgc tgcagcaaaa gctgccgcag aggcagatga tattttcggt gagctaagct
    18481 ctggtaagaa tgcaccgaaa acggggggag gggcgaaagg gaacaatgct tcgcctgccg
    18541 ggagtggtaa tactaaaaac aatggcgcat caggggccga tatcaataac tatgccgggc
    18601 agattaaatc tgctatcgaa agtaagttct atgacgcatc gtcctatgca ggcaaaacct
    18661 gtacgctgcg cataaaactg gcacccgatg gtatgttact ggatatcaaa cctgaaggtg
    18721 gcgatcccgc actttgtcag gctgcgttgg cagcagctaa acttgcgaag atcccgaaac
    18781 caccaagcca ggcagtatat gaagtgttca aaaacgcgcc attggacttc aaaccgtaat
    18841 cgcgatgttg actgttcgga cggtcaacat caggcaccgg ttgccacggg gttctggtag
    18901 ttttgtgtat tttagtttgt taacattctg ctaaattatc gtgggccatc ggtccagata
    18961 agggagatat gatgaagcag gcattacgag tagcatttgg ttttctcata ctgtgggcat
    19021 cagttctgca tgctgaagtc cgcattgtga tcgacagcgg tgtagattcc ggtcgtccta
    19081 ttggtgttgt tcctttccag tgggcggggc ctggtgcggc acctgaagat attggcggca
    19141 tcgttgctgc tgacttgcgt aacagcggta aatttaatcc gttagatcgc gctcgtctgc
    19201 cacagcagcc gggtagtgcg caggaagtac aaccagctgc atggtccgca ctgggcattg
    19261 acgctgtagt tgtcggtcaa gtcactccga atccggatgg ttcttacaat gttgcttatc
    19321 aacttgttga cactggcggc gcaccgggta ctgtacttgc tcagaactcg tacaaagtga
    19381 acaagcagtg gctgcgttat gctggtcata ccgccagtga tgaagtgttt gaaaaactga
    19441 ccggcattaa aggtgcgttc cgtacccgta ttgcctacgt tgttcagacc aacggcggtc
    19501 agttcccgta tgaactgcgc gtatctgact atgacggtta caaccagttt gtcgttcacc
    19561 gttcaccgca gccgctgatg tctccggcgt ggtcaccaga cggttctaaa ctggcttatg
    19621 tgaccttcga aagcggtcgt tccgcgctgg ttattcagac gctggcaaat ggcgctgtac
    19681 gtcaggtggc ttcattcccg cgtcacaacg gtgcacctgc attctcgcca gacggcagca
    19741 aactggcatt cgccttgtcg aaaaccggta gtctgaacct gtacgtaatg gatttggctt
    19801 ctggtcagat ccgccaggtg actgatggtc gcagtaacaa taccgaaccg acctggttcc
    19861 cggatagcca gaacctggca tttacttctg accaggccgg tcgtccgcag gtttataaag
    19921 tgaatatcaa cggcggtgcg ccacaacgta ttacctggga aggttcgcag aaccaggatg
    19981 cggatgtcag cagcgacggt aaatttatgg taatggtcag ctccaatggt gggcagcagc
    20041 acattgccaa acaagatctg gcaacgggag gcgtacaagt tctgtcgtcc acgttcctgg
    20101 atgaaacgcc aagtctggca cctaacggca ctatggtaat ctacagctct tctcagggga
    20161 tgggatccgt gctgaatttg gtttctacag atgggcgttt caaagcgcgt cttccggcaa
    20221 ctgatggaca ggtcaaattc cctgcctggt cgccgtatct gtgataataa ttaattgaat
    20281 agtaaaggaa tcattgaaat gcaactgaac aaagtgctga aagggctgat gattgctctg
    20341 cctgttatgg caattgcggc atgttcttcc aacaagaacg ccagcaatga cggcagcgaa
    20401 ggcatgctgg gtgccggcac tggtatggat gcgaacggcg gcaacggcaa catgtcttcc
    20461 gaagagcagg ctcgtctgca aatgcaacag ctgcagcaga acaacatcgt ttacttcgat
    20521 ctggacaagt acgatatccg ttctgacttc gctcaaatgc tggatgcaca tgcaaacttc
    20581 ctgcgtagca acccgtctta caaagtcacc gtagaaggtc acgcggacga acgtggtact
    20641 ccggaataca acatctccct gggtgaacgt cgtgcgaacg ccgttaagat gtacctgcag
    20701 ggtaaaggcg tttctgcaga ccagatctcc atcgtttctt acggtaaaga aaaacctgca
    20761 gtactgggtc atgacgaagc ggcatactcc aaaaaccgtc gtgcggtact ggtttactaa
    20821 gagaattgca tgagcagtaa cttcagacat caactattga gtctgtcgtt actggttggt
    20881 atagcggccc cctgggccgc ttttgctcag gcaccaatca gtagtgtcag ctcaggctcg
    20941 gtcgaagacc gcgtcactca acttgagcgt atttctaatg ctcacagcca gcttttaacc
    21001 caactccagc aacaactttc tgataatcaa tccgatattg attccctgcg tggtcagatt
    21061 caggaaaatc agtatcaact gaatcaggtc gtggagcggc aaaagcagat cctgttgcag
    21121 atcgacagcc tcagcagcgg tggtgcagcg gcgcaatcaa ccagcagcga tcaaagcggt
    21181 gcgacggcat caacgacgcc gacagctgat gctggtactg cgaatgctgg cgcgccggtg
    21241 aaaagcggcg atgcaaacac ggattacaat gcagctattg cgctggtgca ggataaatcc
    21301 cgccaggatg acgcaatggt ggcatttcag aatttcatca aaaattaccc tgattcaact
    21361 tacctgccaa acgccaatta ttggctgggt cagttaaact acaacaaggg taaaaaagat
    21421 gatgcggcgt actattttgc ttcggtagtg aaaaactatc cgaagtcacc aaaggctgct
    21481 gatgcgatgt ttaaagtcgg cgtcatcatg caggacaaag gtgacaccgc aaaagcgaaa
    21541 gccgtatacc agcaggttat cagtaaatac cctggtactg atggcgctaa acaggcacaa
    21601 aaacgtctga acgcgatgta atgcataaca cacgaccaga agtcgcatta tttctggtcg
    21661 tgtcgtgcga atcataagca gttgagtgat ctgcatcgaa atttttgttg cgctcaagtc
    21721 tgaaatcagt aatatatgcc gccgttgcca cgggatatca aacaaaccga aagcaacgaa
    21781 aaagtgggtc gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt
    21841 cgaatcctgc acgacccacc aatcgctaag gtggaagcgg tagtaaaacg tgaaggataa
    21901 cgttgcatca gcaacggcgc cgaagggcga gacgaagtcg agtcatcctg cacgaccccc
    21961 actaacatag ttagttgtag tatccagcgt agt
//
LOCUS       DAZSNM010000050        21842 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZSNM010000050 DAZSNM010000000
VERSION     DAZSNM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21842
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.050"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..690
                     /gene="prmB"
                     /locus_tag="ACHW42_001599"
     CDS             <1..690
                     /gene="prmB"
                     /locus_tag="ACHW42_001599"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR8943466.1"
                     /translation="RRVNERIPVAYLTNKAWFCGHEFYVDERVLVPRSPIGELINNKF
                     AGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVDAVDISPDALAVAEQNIEEHGLIH
                     NVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSDLPNEYRHEPELGLASGTDGLKLT
                     RRILGNAADYLADDGVLICEVGNSMVHLMEQYPDVPFTWLEFDNGGDGVFMLTKEQLI
                     AAREHFAIYKD"
     gene            725..1810
                     /gene="aroC"
                     /locus_tag="ACHW42_001600"
     CDS             725..1810
                     /gene="aroC"
                     /locus_tag="ACHW42_001600"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR8943467.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            1814..2638
                     /gene="mepA"
                     /locus_tag="ACHW42_001601"
     CDS             1814..2638
                     /gene="mepA"
                     /locus_tag="ACHW42_001601"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR8943468.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            2638..3447
                     /locus_tag="ACHW42_001602"
     CDS             2638..3447
                     /locus_tag="ACHW42_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFR8943469.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            3447..3995
                     /gene="epmC"
                     /locus_tag="ACHW42_001603"
     CDS             3447..3995
                     /gene="epmC"
                     /locus_tag="ACHW42_001603"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR8943470.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            4029..4307
                     /locus_tag="ACHW42_001604"
     CDS             4029..4307
                     /locus_tag="ACHW42_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR8943471.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(4428..6434)
                     /gene="mnmC"
                     /locus_tag="ACHW42_001605"
     CDS             complement(4428..6434)
                     /gene="mnmC"
                     /locus_tag="ACHW42_001605"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR8943472.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            6593..7834
                     /gene="fabB"
                     /locus_tag="ACHW42_001606"
     CDS             6593..7834
                     /gene="fabB"
                     /locus_tag="ACHW42_001606"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR8943473.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            8089..9267
                     /locus_tag="ACHW42_001607"
     CDS             8089..9267
                     /locus_tag="ACHW42_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8943474.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(9264..10259)
                     /gene="flk"
                     /locus_tag="ACHW42_001608"
     CDS             complement(9264..10259)
                     /gene="flk"
                     /locus_tag="ACHW42_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR8943475.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLTAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            10358..11494
                     /gene="pdxB"
                     /locus_tag="ACHW42_001609"
     CDS             10358..11494
                     /gene="pdxB"
                     /locus_tag="ACHW42_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR8943476.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGLLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            11560..12573
                     /locus_tag="ACHW42_001610"
     CDS             11560..12573
                     /locus_tag="ACHW42_001610"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR8943477.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            12573..13385
                     /gene="truA"
                     /locus_tag="ACHW42_001611"
     CDS             12573..13385
                     /gene="truA"
                     /locus_tag="ACHW42_001611"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR8943478.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            13468..14127
                     /locus_tag="ACHW42_001612"
     CDS             13468..14127
                     /locus_tag="ACHW42_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR8943479.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            14283..15197
                     /gene="accD"
                     /locus_tag="ACHW42_001613"
     CDS             14283..15197
                     /gene="accD"
                     /locus_tag="ACHW42_001613"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR8943480.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            15267..16535
                     /gene="folC"
                     /locus_tag="ACHW42_001614"
     CDS             15267..16535
                     /gene="folC"
                     /locus_tag="ACHW42_001614"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR8943481.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            16525..17187
                     /gene="dedD"
                     /locus_tag="ACHW42_001615"
     CDS             16525..17187
                     /gene="dedD"
                     /locus_tag="ACHW42_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR8943482.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            17446..17934
                     /gene="cvpA"
                     /locus_tag="ACHW42_001616"
     CDS             17446..17934
                     /gene="cvpA"
                     /locus_tag="ACHW42_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR8943483.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            17971..19488
                     /gene="purF"
                     /locus_tag="ACHW42_001617"
     CDS             17971..19488
                     /gene="purF"
                     /locus_tag="ACHW42_001617"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR8943484.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            19583..20152
                     /gene="ubiX"
                     /locus_tag="ACHW42_001618"
     CDS             19583..20152
                     /gene="ubiX"
                     /locus_tag="ACHW42_001618"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR8943485.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            20418..21200
                     /gene="argT"
                     /locus_tag="ACHW42_001619"
     CDS             20418..21200
                     /gene="argT"
                     /locus_tag="ACHW42_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR8943486.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            21421..>21842
                     /locus_tag="ACHW42_001620"
     CDS             21421..>21842
                     /locus_tag="ACHW42_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFR8943487.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQG"
BASE COUNT         4933 a         5561 c         6053 g         5295 t
ORIGIN      
        1 cgccgcgtca acgagcgcat tccggtggct tacctgacca acaaagcctg gttctgcggt
       61 catgaatttt acgtcgatga acgcgtgctg gtgccgcgct cgccgattgg tgaactgatc
      121 aacaataaat ttgccggact catcagcaag caaccgcagc atattttaga tatgtgtacc
      181 ggtagcggct gcatcgccat tgcctgtgct tatgccttcc cggaagcgga agtcgacgcg
      241 gtagatatct ctcccgacgc gctggcggtt gccgaacaga acatcgaaga acacggtctg
      301 atccacaacg tcattccgat tcgttccgat ctgttccgtg atttgccgaa agtgcaatac
      361 gatctgattg tcactaaccc gccgtatgtc gacgcggaag atatgtccga cctgccaaac
      421 gaataccgtc acgagccgga actgggcctg gcatctggca ccgacggcct gaaactgacg
      481 cgtcgcattc tcggtaacgc ggcagattac cttgctgatg atggcgtgtt gatttgtgaa
      541 gtgggcaaca gcatggtaca tcttatggaa caatatcccg atgttccgtt cacctggctg
      601 gagtttgata acggcggcga tggtgtgttt atgctcacta aagagcagct tattgccgca
      661 cgagaacatt tcgcgattta taaagattaa gtaaacacgc aaacacaaca ataacggagc
      721 cgtgatggct ggaaacacaa ttggacaact ctttcgcgta accaccttcg gcgaatcgca
      781 cgggctggcg ctcggctgca tcgtcgatgg tgttccgcca ggcattccgc tgacggaagc
      841 ggacctgcaa catgaccttg atcgtcgtcg ccctgggaca tcgcgctata ccacccagcg
      901 ccgcgagccg gatcaggtca aaatactctc cggtgttttt gaaggcgtta ctactggcac
      961 cagcattggc ttgttgatcg aaaataccga ccagcgttct caggattaca gcgcaattaa
     1021 agacgtcttc cgtccaggcc atgctgatta cacctacgaa caaaaatacg gtctgcgcga
     1081 ttatcgcggc ggtggacgtt cttccgcccg cgaaaccgcc atgcgcgtgg cggcaggggc
     1141 gattgccaaa aaatatctcg ccgagaaatt tggtattgaa atccgcggct gcctgaccca
     1201 gatgggcgac attccgctgg aaatcaaaga ctggtcgctg gttgaacaaa atccgttctt
     1261 ctgcccggac cccgacaaaa tcgacgcgtt agatgaactg atgcgcgcgc tgaaaaaaga
     1321 gggcgactcc atcggcgcga aagtcaccgt tgttgccagt ggtgtccccg ccggacttgg
     1381 cgagccggtc tttgaccgcc tggatgccga catcgcccat gcgctgatga gcatcaacgc
     1441 ggtgaaaggc gtggaaattg gcgacggttt tgacgtagtg gcgctgcgcg gcagccagaa
     1501 ccgcgacgaa atcaccaaag acggtttcca gagcaaccat gcgggcggca ttttcggcgg
     1561 tatcagcagc gggcagcaaa tcattgctca tatggcgctg aaaccgacct ccagcattac
     1621 cgtgccgggg cgcactatta accgctttgg cgaagaagtt gagatgatca ccaaaggccg
     1681 tcacgatccc tgtgtcggga tccgtgcagt gccgatcgct gaagcgatgc tggcgatcgt
     1741 tttaatggat cacctgttac ggcagcgggc gcaaaatgcc gatgtgaaga ctgatattcc
     1801 acgctggtaa aaaatgaaca aaaccgcgat tgcgctgctg gctctgcttg ccagtagtgc
     1861 tagcctggca gcgacgccgt ggcaaaaaat aacccaacct gtgccgggta gcgcacaatc
     1921 gataggcagt ttttctaatg gctgtattgt cggcgctgac acgttgccga tacagtccga
     1981 acattatcag gtcatgcgta ccgatcagcg tcgctatttc ggtcacccgg atctggtgat
     2041 gtttatccag cgtctgagta gccaggtgag caatctgggc atgggtacgg tgctgattgg
     2101 cgatatgggg atgcccgctg gtgggcgttt caacggcggt catgccagcc accagaccgg
     2161 actggatgtc gatatctttc tgcaactgcc gaaaactcgc tggacctccg cgcagctctt
     2221 gcgcccgcaa gcactggact tagtttcccg cgacggtaaa cacgttgtct ccacgctgtg
     2281 gaagccagaa attttcagct tgatcaaact cgccgcccag gacaaagacg tcacgcgcat
     2341 ttttgttaat ccggcgatta aacaacaact ttgccttgat gcgggcaccg atcgcgactg
     2401 gttgcgcaaa gtgcgaccct ggttccagca tcgcgcgcat atgcatgtac gattacgttg
     2461 ccctgccgat agtctggagt gtgaagatca acctttaccg ccatcaggcg atggttgcgg
     2521 ggcagaactg caaagctggt ttgaacctcc aaaaccggga acaacaaagc ctgagaagaa
     2581 gacaccgcct ccgttgccgc cttcctgcca ggcgctactg gatgagcacg tgatctaatg
     2641 gaaacgttta atagcctgtt tatggtttct ccgctgttgc tgggagttct cttttttgtc
     2701 gccatgctgg cgggatttat cgactcgatt gccggtggcg gtgggttact caccattccg
     2761 gcattgatgg ctgcgggaat gtctcccgct aacgcgctgg caaccaataa actgcaagcc
     2821 tgcggcggct ctatttccgc cactatctac tttattcgcc gcaaagtggt tagtttaagc
     2881 gatcagaaac tcaatattgc catgaccttt gtcggctcaa tgagcggcgc actgttggta
     2941 cagtacgttc aggctgatgt cttacggcag attttgccca ttctggtgat ttgtatcggc
     3001 ctctatttct tgctgatgcc aaagctgggt gaagaagatc gccagcgccg gatgtacggt
     3061 ttacccttcg cgttaattgc cggtggctgc gtcggttttt atgacggatt ctttggcccg
     3121 gctgccggat cgttttacgc gctggccttc gttatgctgt gcggattcaa cctcgccaaa
     3181 gccacggctc acgccaaatt actcaacgca acgtcaaaca ttggcggtct gctgctgttt
     3241 attctcggcg gcaaagtgat ttgggcgacg ggctttgtca tgctggtggg gcagttcctc
     3301 ggcgcgcgca tgggttcacg gttggtgttg agcaaaggac aaaagctgat ccgcccgatg
     3361 attgttatcg tctcggcggt gatgagtgcc aaattacttt acgatagcca cggacaggag
     3421 atcctccact ggttggggat gaactaatga acagtacaca ccactacgag cagttgattg
     3481 aaatttttaa tagctgcttt gccgatgaat ttaatacccg tctgattaaa ggcgacgacg
     3541 aaccgatcta tcttcctgct gatgcggaag taccgtataa ccgaatcgtc tttgctcatg
     3601 gcttttatgc cagcgcaatt catgagattt cgcactggtg tattgccgga aaagcgcgcc
     3661 gtgagctggt tgatttcggc tactggtatt gcccggacgg acgcgatgcc cagacgcaaa
     3721 gccagtttga agatgttgaa gtgaagccgc aggcgttcga ctggttattc tgcgtggcgg
     3781 ctggatatcc gtttaacgtc agttgcgaca atctggaagg ggatttcgaa ccggatcgtg
     3841 tggtgtttca acggcgggta catgcgcagg tgatggatta tctgacaaat ggcattccgg
     3901 aacgcccggc acgcttcatt aaagcactac aaaattatta tcacacgccg gaacttacgg
     3961 cggaacagtt cccgtggccg gaagcgctca actgaagcca gcgccacgtt ttacaatgag
     4021 gaaagaagat gatcgcggag tttgaatcac gcattctggc attaatcgac ggcatggttg
     4081 accatgccag tgatgatgag ttgtttgcca gtgggtattt gcgtggccac ctgacattag
     4141 ccatcgcaga actggaaagt ggtgatgacc actccgctca ggcggtgcat acgaccgtta
     4201 gccagagtct ggaaaaagcc attggtgccg gtgaattgtc gccgcgtgac caggcgctgg
     4261 tgaccgatat gtgggaaaac ctgtttcagc aggcgtcaca gctgtaagtt gcggcgtctg
     4321 gagcaccatg cctgatgcga cgcttatcgc gtcttatcag gcctacacgt cacacgtagg
     4381 ttggataagg cgttcacgcc gcatccggca aacactgcga gagcagatta ccccgcctta
     4441 accgctttac ccttcaacaa tttccgcacc cataaccgat tcgggtttaa cgccgccagc
     4501 gtactggcat ccatcggaat cggttcgtcg ctcatctgcg ccgccagaat ctcggcacac
     4561 agcggggcag aacacaaacc gcgagaacct aaagccgcaa acataaagag atcgtcaaaa
     4621 accggcgcgc ttaccgcctc atctttttgc tccgccaacg acgcatattc cacgagtgtt
     4681 gcctcataat cgggaacatt gcctaccatt ggcagatgat cgcgggtggc acaacgcaca
     4741 ccgcagcgcg cctctttatc actgacatca acctcttttg cccactgtgc ctgcgggaaa
     4801 caatcaatca accgctggcg attctgctgc tgatcgtcct cactgtacgc cgtatcttcg
     4861 ctgccgcgat gataactggc accaatacaa tgatgttgat tcgccggatt ttgtggcgtg
     4921 agataaccgt catagcacag cacctgcttc agctctgcca gttccggcgt tgtcggaata
     4981 tggctgacct gcccggcaac cgaatacacc gggagagtcg acgtttggct gaaacggctg
     5041 atttgatgcc cgttcgccag taccactacg ctgtgtgttg cttgctgatc tcctgcaaaa
     5101 ttcaacaacc aacagtcatc cttacgggat aaattctgta actgatattg ataataaatc
     5161 tgcaaaccct gctgttgcgc cagttccagc acattacggg tcagttctgc tgggcacagc
     5221 caaccacctt gcggataagt aatgccgctg caatttgtcg caacgcccgt aatttgttca
     5281 accgcatttg cctcaacggc tacagccagt tctgcgggta aatccattga caacatctgt
     5341 gcgattttat gctggctttt ctcatcccag cctaactgcg tgacgccgca ccagtcatga
     5401 tcaaatttaa cgggtaattg gtcgtaaaac cgacgagcaa aagtaaacgc attagagaaa
     5461 aagcggttta gcgcctcatc gtgtttgctt aataacggat acagcgcccc ctggcgattg
     5521 ccggaagcac ccagcgcggg ggcttcatcc gcgcaataaa gcgctacctg ccagccgcgc
     5581 cgtaataacg ccagcgacaa caacgcgctg gcaataccac cgccgataat cgctacttcc
     5641 cgtttgctgc tgcccgtacg attaaaccac ggcgtggagc aggggagtgg taatgtctgt
     5701 tccatcaccc cacaaagcat ttcccgtttg cgcccaaagc ccttacgttt ttgcatcgtg
     5761 aatccggctt cctgcaaacc gcggcggaca aaaccggcag acgtaaatgt cgccagcgtg
     5821 ccgcctggac gcgccaacct tgccatggcg ttaaacagat tttgcgtcca catatccggg
     5881 tttttagctg gcgcaaagcc gtccagaaac caggcatcta ctttttgatt tagcgaatcg
     5941 tccagttggc tggtcagttc gttaatatcg ccaaaccata aatccagcgt cacgcggctt
     6001 tcatcgagca ataaacgatg gcaaccgggc aagggcattg gccactgcgc ctgaagttgt
     6061 tctgcccacg gagccagttc cggccagtgt tgatgcgcta aggctaaatc cgcacgggtg
     6121 aggggaaatt tctcaaaact aatgaaatgt aagcgttgta attgcgcttg cggatgcgct
     6181 tcgcgaaact gatcaaatgc cttccatagc gtcaggaagt ttaatccggt gccgaagccg
     6241 ctctctgcta tcacaaacag aggatgtgga tgctcaggaa agcgtacctc taattggtta
     6301 cctcccagaa aaacataacg cgtctcttcc agcccgttat cgttggaaaa atagacatcg
     6361 tcaaaatctc gggaaacagg tgtaccctca gcattaaatt cgaggttggc aggttgtatg
     6421 gagtagtgtt tcacgtaagt tactcgtctt acaggcggtg gctcgatctt agcgatgtgt
     6481 gtaaggctgc gcaaatatct ctattaaatg gctgatcgga cttgttcggc gtacaagtgt
     6541 acgctattgt gcattcgaaa cttactctat gtgcgactta cagaggtatt gaatgaaacg
     6601 tgcagtgatt actggcctgg gcattgtttc cagcatcggt aataaccagc aggaagtcct
     6661 ggcatctctg cgtgaaggac gttcagggat cactttctct caggagctga aggattccgg
     6721 catgcgtagc cacgtctggg gcaacataaa actggatacc actggcctca ttgaccgcaa
     6781 agttgtgcgc tttatgagcg acgcatccat ttatgcattc ctttctatgg agcaggcaat
     6841 cgctgatgcg ggcctctctc cggaagctta ccagaataac ccgcgcgttg gcctgattgc
     6901 aggttccggc ggcggttccc cgcgtttcca ggtgttcggc gctgacgcaa tgcgcggccc
     6961 gcgcggcctg aaagcggttg gcccgtatgt ggtcactaaa gcgatggcat ccggcgtttc
     7021 tgcctgtctc gccacgccgt ttaaaattca tggcgttaac tactccatca gctccgcgtg
     7081 tgcgacttcc gcacactgta tcggtaacgc agtagagcag atccaactgg gcaaacagga
     7141 catcgtgttt gctggcggcg gcgaagagct gtgctgggaa atggcctgcg aattcgacgc
     7201 gatgggtgcg ctatctacta aatacaacga caccccggaa aaagcctccc gtacttacga
     7261 cgctcaccgt gacggtttcg ttatcgctgg cggcggcggt atggtagtgg ttgaagagct
     7321 ggaacacgcg ctggcgcgtg gtgcttacat ctatgctgaa atcgttggct acggcgcaac
     7381 ctctgatggt gcagacatgg ttgctccgtc tggcgaaggc gcagtacgtt gtatgcagat
     7441 ggcgatgcat ggcgttgata ccccaatcga ttacctgaac tctcacggta cttcgactcc
     7501 ggttggcgac gtgaaagagc tggcagctat ccgtgaagtg ttcggcgata agagcccggc
     7561 gatttctgca accaaagcca tgaccggtca ctcactgggc gctgcgggtg tgcaggaagc
     7621 tatctactct ctgctgatgc tggaacacgg ctttatcgcc ccgagcatca acattgaaga
     7681 gctggacgag caggctgcgg gtctgaacat cgtgaccgaa acgaccgatc gcgaactgac
     7741 cactgttatg tctaacagct tcggcttcgg cggcaccaac gccacgctgg taatgcgcaa
     7801 gctgaaaaga ttaattcgca gtaggccgga gtaggtcgga taagacgcgc cagcgtcgca
     7861 tccgacgtta cgcgccaatg cggcctccgg cactaacgca aaagggaacc tgatggttcc
     7921 ctttttcaca tcattgacaa tcgccaccag ttctaggcaa acttcccgct ttgtcgattt
     7981 ccttctgaaa agacgtacgc gttaaatcct gccaacgcac cgtaaccctg aaaccagaga
     8041 gatgagacgg ggatactcct cgccttgcgc tgcattctgg agtaatgcat gactgctgta
     8101 agccaaaccg aaacacgatc ttctgccagt ttttcgctct tccgcatctc ttttgcggtt
     8161 tttctcacct acatgaccgt agggttgccg ttgccggtta tcccgctgtt tgttcatcat
     8221 gatctgggct atggcaatac catggtcggc attgccgtcg ggattcagtt tctggctacg
     8281 gtgctgacgc gtggttacgc cgggcgactg gccgatcaat atggcgcaaa acgttcggcg
     8341 cttcagggga tgttagcttg tggtctggct ggcggcgcgt tgctgctggc ggcgattttg
     8401 cctgtctccg caccgttcaa atttgccctg ttggtcatcg ggcgtttgat tcttgggttt
     8461 ggtgaaagcc agttactgac aggcgctctg acctgggggt taggcatcgt agggccaaaa
     8521 cactctggca aagtgatgtc atggaacgga atggcgattt acggtgccct cgctgttggt
     8581 gcgccgcttg gcctgttgat tcatagccat tacggttttg ccgcactggc gatcaccaca
     8641 atggtattac ccttactggc gtgggcctgt aacggcacag tgcgcaaagt accggccctg
     8701 gcgggagaac gtccatcgct gtggagcgtt gtcgggctta tctggaaacc agggttaggg
     8761 ctggcactac aaggcgttgg ttttgcggtt atcgggactt tcgtttcgct ctactttgcc
     8821 agcaaaggat gggcgatggc gggctttact cttaccgcgt ttggcggcgc atttgtcgtg
     8881 atgcgcgtca tgtttggctg gatgccggac cgttttggcg gcgtgaaagt ggcgattgtc
     8941 tctctgcttg tagaaacggt gggcttgttg ctgctctggc aagccccagg tgcatgggtc
     9001 gcattagcgg gcgcggcgtt aaccggagcc ggatgttcgc ttatctttcc tgcgctgggc
     9061 gtggaggtgg ttaaacgcgt cccctcacaa gttcgcggca ccgcactggg cggttacgcc
     9121 gcgtttcagg atatcgccct cggcgtctcc gggccgctgg cgggaatgct ggcgaccacg
     9181 tttggttact cttcggtatt tcttgccggg gcgatctctg cggtgctggg tattattgtc
     9241 acgatactgt catttcgtcg gggttaacga accagccaga ccagcatcag gataatcgcg
     9301 actaacagta tccacagcgc gggacgcgtt gccatatttt gcaacgtgtc catcatcggt
     9361 ggaaaaggat taagcagcgg ttgcatatca cgcggatcaa tccctttaac ccgccgttga
     9421 taaagctgat taagaatatc ctcggcctgg gcagaagaaa gcgatgattg cgcagaaagg
     9481 ccatattttt gctggatata tgccgataac gccgccagtt cactggcatc taaaggttgt
     9541 tttagcgcca tctgtagtga ttccagcgtc ggcgtatttt gctggcttag cgtctgacgc
     9601 gcctgtagcc aggtcaccag atggttaaac agtttcgctg gaattaactc gccatctttc
     9661 accccggaaa gttccagcat cgattgccag atctgtttgc tgggttcccc cgttgccgcc
     9721 gtaagtttgg tcaccagctg ttttagcgca ttgtgctccg ccggtaataa aggacggtcg
     9781 gtcgcctcgc gctgctgtgg ttgcggaata accatcttcc cttcctgcaa cagggtgaga
     9841 atggttttta attgctccgg tgagagctga ttcagcggcg tctgaccaaa gttatgacgg
     9901 atataatccg tgaccgcctg acgatgattc cccagacgta aatactcccc taattgcgct
     9961 aaaagctggc gggcagaatg gcttttttgc gcggccagca gacgttgcgc cagattatgc
    10021 tcagcggcag ggaagtgacg cgaaagcagc ggtgaatctc ccgacagacc aatatcgtgc
    10081 ctgatacccg cccacagttc tgctctttgt tgttgcgtca gtgaggtcac tttcgtcatt
    10141 aagctttcca gcgaagtacg ttgctgactg gataaaggct gattgcccgc gccagacggc
    10201 aggttatctc cctgacctgg tggctgccca ggaggagggc cggaaatagg ttgtatcatt
    10261 acgtatcctt atacctgaaa tcttcgcaag tatgcctggc cgcgagatta tggcatactt
    10321 gtccggttaa ctctcgtctc atacaggtaa cacaaacgtg aaaatccttg ttgatgaaaa
    10381 tatgccttat gcccgcgact tatttagccg tttgggtgag gtgatcgcgg ttcccgggcg
    10441 tccaatcccc gtcgctcaac tggcagacgc ggatgcgctg atggtgcgtt cggtcacgaa
    10501 agtgaatgaa tctttgctgg caggaaaacc cattaaattt gttggaactg ccactgcggg
    10561 gaccgaccat gtcgatgaag catggttgaa gcaggcggga attggttttt ccgctgcacc
    10621 tggctgtaat gcgattgcgg tggtggaata tgttttctcc tccctgctga tgcttgccga
    10681 acgcgatgga ttttcactgc acgaccgtac ggtggggatc gtgggcgttg gtaacgttgg
    10741 acgtcgattg caggcgcgac tggaagcgtt agggattaaa accttacttt gcgatccgcc
    10801 tcgcgccgac cgtggggatg agggtgattt ccgctcgctg gatgagttag tccagcgcgc
    10861 ggatattctg actttccata cgccactctt taaagatggt ccgtacaaaa cgctacatct
    10921 ggcggatgaa aaactgatcc gtagcctgaa gcccggagcg attctgatta acgcctgccg
    10981 tggcgcagtc gtcgataata ccgcgctgct gacctgcctg aatgaaggcc agaagttaag
    11041 cgtggtgctg gatgtctggg aaggcgagcc ggaacttaat gtggaattgc tgacaaaagt
    11101 ggatatcggc acgccgcata tcgcaggcta taccctggag ggtaaagcgc gtggcacgac
    11161 gcaagtgttt gaagcctata gcaagtttat tgggcatgaa caacacgttg cgctggatac
    11221 gttgctgccc gcgcctgagt ttggccgcat tacgctgcat ggtctgctcg atcaaccgac
    11281 gctgaaaagg ctggtgcatt tggtgtatga tgtgcgccgc gatgacgcac cgctgcgtaa
    11341 agtcgccggg ataccgggtg agttcgataa actgcgcaaa aactatcttg agcgccgtga
    11401 atggtcatct ctgtatgtaa tttgtgatga cgccagtgcg gcatcattgc tgtgtaaact
    11461 gggttttaac gccgttcatc atccggcacg ttaatctctt cttcatgctc tctgctgtaa
    11521 cattggcagg gagctttgct atttctggag taaaccacca tgtctgaagg ctggaacatt
    11581 gccgtcctgg gcgcaactgg cgctatgggc gaagccctgc ttgaaacgct ggctgaacgt
    11641 cagttcccgg ttggggaaat ttatgcactg gcacgtaacg aaagcgcagg cgaacaactg
    11701 cgctttggtg gtaaaacaat caccgtgcag gatgccgctg aattcgactg gacgcaggcg
    11761 cagctggcat tttttgtcgc aggcaaagaa gctaccgctg cctgggttga agaagcgacc
    11821 aactcaggtt gcctggtgat cgacagcagc ggactgtttg ctctcgaacc cgacgtaccg
    11881 ctggtggtgc cggaagtaaa cccgtttgta ctgaccgatt accgcaaccg gaatgtcatc
    11941 gccgtaccag acagtctgac cagccagctg ctggcggcac tgaaaccgtt aatcgatcag
    12001 ggcggtttgt cgcgtatcag tgttaccagc ctgatttcag cctccgccca gggcaaaaaa
    12061 gcggtcgatg cgttagcggg gcagagtgcg aaattgctca acggcattcc gattgacgaa
    12121 gaagatttct tcggtcgtca actggcgttc aatatgctgc cgttgttgcc ggatagcgaa
    12181 ggtagcgtgc gtgaagaacg tcgtatcgtt gacgaagtac gcaaaatcct gcaggacgaa
    12241 gggctgatga tttcggcaag cgtcgtccag gcaccggtat tctacggtca tgcccagatg
    12301 gtcaactttg aagcactgcg tccgctggcg gcagaagaag cgcgtgatgc gtttgctcag
    12361 ggcgaagata ttgtgctctc tgaagagaac gaattcccga ctcaggtagg ggatgcttcg
    12421 ggtacgccgc atctttctgt tggctgcgtg cgtaatgact acggtatgcc ggagcaagtc
    12481 cagttctggt cggtggccga taacgttcgc tttggcggcg cgctgatggc agtaaaaatc
    12541 gccgagaaac tggtgcagga gtatctgtac taatgtccga ccagcaacaa ctgccagttt
    12601 ataaaattgc gctgggcatt gagtacgacg gcagtaagta ttacggctgg caacggcaga
    12661 atgaagtccg cagtgtgcag gagaagctgg aaaaggcgct ctcccaggtg gcgaacgaac
    12721 ccatcaccgt cttctgcgcc gggcgtactg acgcaggggt acacggtacc gggcaagtgg
    12781 tgcatttcga aacaaccgcg cagcgcaaag acgcggcgtg gaccttgggc gtaaatgcga
    12841 atttacctgg tgacatcgcc gtgcgttggg ttaaagctgt acctgatgat tttcatgccc
    12901 gatttagcgc cacggctcgc cgttatcgct acatcatcta caatcatcgg ctgcgcccgg
    12961 cggtactgag taaaggggta acccattttt acgaaccgct ggacgctgaa cggatgcatc
    13021 gggctgcgca atgcttgctg ggcgagaatg atttcacctc gttccgtgcg gtgcagtgcc
    13081 agtcccgaac cccgtggcgc aacgttatgc atattaatgt cacgcgtcac ggtccttatg
    13141 tggtggttga tatcaaagcg aatgcctttg tacatcatat ggtcaggaat attgtcggca
    13201 gcctgatgga agtcggtgcc cacaaccagc cggagagctg gatagcagag ttgctggcgg
    13261 caaaggacag aacgctggcg gcagcaacgg caaaagcgga agggctgtat ctggtcgcgg
    13321 tggattaccc tgaccggtat gatcttccaa aaccgccaat gggcccgcta tttctggcgg
    13381 actaacgaga ataatgctcg gaacatttca ggctgtagcc aacgtagtta cagcctgaaa
    13441 gatgacgagt acaaggcata ggcaaatatg gacctgattt atttcctcat cgattttatc
    13501 ctgcacattg acgtgcatct ggcagagcta gtcgcggagt acggcgtctg ggtttatgcc
    13561 attttgttct taattttgtt ctgtgaaacc ggtctggtgg taatgccgtt tttaccgggt
    13621 gattcattgc tgtttgttgc cggagcgttg gcatcactgg aaaccaacga tctcaacgtc
    13681 catatgatgg tagtgctgat gctgatcgcc gcgattgtgg gtgacgcggt caattacact
    13741 atcgggcggt tgttcggtga aaagttattc agtaatccca attcgaaaat tttccgtcgc
    13801 agttatctcg acaaaaccca tcagttttat gagaaacatg gcggcaaaac gattattctc
    13861 gcccgttttg tgccgatcgt cagaacgttc gccccgtttg ttgcgggaat gggccacatg
    13921 tcgtaccgtc atttcgctgc ttataacgtg atcggcgcac tgttgtgggt actgcttttt
    13981 acctacgcag gctatttctt cggtacaatc ccgatggttc aggataacct taagctgctg
    14041 atcgtcggga ttattgtggt ttctattttg ccgggcgtca tcgaaataat ccgtcacaaa
    14101 cgcgctgcgg cacgcgccgc aaaataaaaa gtaactccgc ggttcgacca cttttttatc
    14161 caaagtttcg ggctgttatg ttttaatgtg caacattcat ggtctgttgg gggcaaaaat
    14221 ggcattatgc gtccccaaag ataaaactgg catcgaacca ggttcagaca gaaaggtccc
    14281 taatgagctg gattgaacga attaaaagca acattactcc cacccgcaag gcgagcattc
    14341 ctgaaggggt gtggactaag tgtgatagct gcggtcaggt tttataccgc gctgagctgg
    14401 aacgtaatct tgaggtctgt ccgaagtgtg accatcacat gcgtatgaca gcgcgtaatc
    14461 gcctgcatag cctgttagat gaaggaagcc ttgtggagct gggtagcgag cttgagccga
    14521 aagatgagct gaagtttcgt gactccaaga agtataaaga ccgtctggca tctgcgcaga
    14581 aagaaaccgg cgaaaaagat gcgctggtgg tgatgaaagg cactctgtat ggaatgccgg
    14641 ttgtcgctgc ggcattcgag ttcgccttta tgggcggttc aatggggtct gttgtgggtg
    14701 cacgtttcgt gcgtgccgtt gagcaggcgc aggaagataa ctgcccgctg atctgcttct
    14761 ccgcctctgg tggcgcacgt atgcaggaag cactgatgtc gctgatgcag atggcgaaaa
    14821 cctctgcggc actggcaaaa atgcaggagc gcggcttgcc gtacatctcc gtgctgaccg
    14881 acccgacgat gggcggtgtt tctgcaagtt tcgccatgct gggcgatctc aacatcgctg
    14941 aacctaaagc gttaatcggc tttgccggtc cgcgtgttat cgaacagacc gttcgcgaaa
    15001 aactgccgcc tagattccag cgcagtgaat tcctgatcga gaaaggcgcg atcgacatga
    15061 tcgtccgtcg tccggaaatg cgcctgaaac tggcgagcat tctggcgaag ttgatgaatc
    15121 tgccagcgcc gaatcctgaa gcgccgcgtg aaggcgtagt ggtacccccg gtaccggatc
    15181 aggaacctga ggcctgataa ctgataaggg cagggccact ggctctgccc ttttgctatt
    15241 ctcaccgtaa cgaatcagcg gataccatga ttatcaaacg cactcctcaa gccgcgtcgc
    15301 ctctggcttc gtggctttct tatctggaaa acctgcacag taaaactatc gatctcggcc
    15361 ttgagcgcgt gagccaggtc gcggcgcgtc ttggcgtcct gaaaccagcg ccatttgtgt
    15421 ttaccgttgc gggtacgaat ggcaaaggca ccacctgccg tacgctggag tcgattctga
    15481 tggcggcagg gtacaaagtg ggcgtctaca gttcgcctca tctggtgcgt tataccgagc
    15541 gcgtacgtgt gcagggccag gaattgccgg aatcggccca caccgcctct tttgcggaga
    15601 ttgaatcggc acgcggtgat atttccctga cctatttcga gtacggtacg ctgtcggcgt
    15661 tgtggctgtt caagcaggca caacttgacg tggtgattct ggaagtaggg ctgggcggtc
    15721 gtctggacgc aaccaatatt gtcgatgccg atgtcgcggt agtaaccagt attgcgctgg
    15781 atcataccga ctggctgggt ccagatcgcg aaagtattgg tctcgagaaa gcaggcatct
    15841 tccgcagcga aaaaccggca attgtcggtg agccggaaat gccttctacc attgctgatg
    15901 tggcgcagga aaaaggtgca ctgttacaac gtcggggcgt tgagtggaac tattccgtca
    15961 ccgatcatga ctgggcgttt agcgatgctc acggcacgct ggaaaatctg ccgttgccgc
    16021 ttgtcccgca accgaatgcc gcaacggcgc tggcggcact gcgtgccagc gggctggaag
    16081 tcagtgaaaa tgccattcgc gacgggattg ccagcgcaat tttgccggga cgtttccaga
    16141 ttgtgagcga gtcgccacgc gttatttttg atgtcgcgca taatccacat gcggcggaat
    16201 atctcaccgg gcgtatgaaa gcgctaccga aaaacgggcg cgtgctggcg gttatcggta
    16261 tgctacatga taaagatatt gccggaactc tggcctggtt gaaaagcgtg gttgatgact
    16321 ggtattgtgc gccactggaa gggccgcgcg gtgccacggc agaacaactg cttgagcatt
    16381 tgggtaacgg caaatcattt gatagcgttg cgcaggcatg ggatgccgca atggcggacg
    16441 ctaaagcgga agacaccgtg ctggtgtgtg gttctttcca cacggtcgca catgtcatgg
    16501 aagtgattga cgcgaggaga agcggtggca agtaagtttc agaatcggtt agtgggcacg
    16561 atcgtgctgg tggcgctggg ggtgattgta cttccagggc tgctggacgg gcagaaaaaa
    16621 cattatcagg atgagttcgc ggctatcccg ctggtgccga aagcgggcga tcgtgatgag
    16681 cctgatatga tgccagccgc cacccaggcg ttgccgacgc agccgccgga aggcgcagcg
    16741 gaagaggtgc gggcaggtga tgccgcagca ccgtcgctcg atccggccac tattgcagcc
    16801 aataacaccg agtttgaacc ggaacctgca ccggtcgccc caccgaagcc gaaaccggtg
    16861 gagccgccta aaccaaaggt tgaagtacca cctgcgccga agccagaacc gaagccggtc
    16921 gtggaagaaa aagcagcgcc aacgggtaaa gcctatgttg tgcaactggg tgcgctgaaa
    16981 aatgccgata aagtgaatga gattgtcggt aagctgcgcg gtgccggtta tcgggtttat
    17041 acgtcgccat ccacgccagt gcagggtaaa attacccgta ttctggttgg gccggatgcc
    17101 tcgaaagata agctgaaagg ttcgctgggt gagttgaagc aactttctgg cttaagtggc
    17161 gtggtaatgg gctatacgcc gaattaatac agtcttgcct gatgcgacgc tggcgcgtct
    17221 tatcaggctt gggcagtgtt agaaccgtag gtcggataag gcgtttacgc cgcatccgac
    17281 acgcattgca cgatgccgca aaggcataaa aagtcgatgg cgttgaatat tttttcagcg
    17341 ccatttttat tgatgcgcgg gaaggaaatc cctacgcaaa cgttttcttt ttctgttaga
    17401 atgcgccccg aacaggatga cagggcgtaa aatcgtggga cacatatggt ctggattgat
    17461 tacgccataa tcgcggtgat tgctttttcc tctctggtta gcctgatcca cggctttgtt
    17521 cgtgaagcgt tatcgctggt gacatggggt tgtgctttct ttgttgccag tcattactac
    17581 acttacctgt cagtctggtt tacgggcttt gaagacgaac tggttcgaaa tgggattgcc
    17641 atcgcggtac tgtttatcgc taccctgatc gttggtgcta tcgtgaactt cgtgataggc
    17701 cagttggtgg agaaaacggg gttgtcaggc accgatcggg tgctgggcgt ctgtttcggt
    17761 gcgttgcgcg gtgtgttgat tgttgccgcc attctcttct ttctcgactc ctttaccggg
    17821 gtgtcgaaaa gtgaagactg gagcaaatca cagctgatcc cgcaattcag ttttatcatc
    17881 agatggtttt ttgattatct gcaaagctcg tcaagtttct tgcccagagc gtaagtgctc
    17941 tgagatgtgg cttaacgagg aaaaagacgt atgtgcggta ttgtcggtat cgccggtgtt
    18001 atgccggtta accagtcgat ttatgatgcc ttaacggtgc ttcagcatcg cggtcaggat
    18061 gccgccggca tcatcaccat agatgccaat aactgcttcc gtttgcgtaa agcgaacggg
    18121 ctggtgagcg atgtatttga agctcgccat atgcagcgtt tgcagggcaa tatgggcatt
    18181 ggtcatgtgc gttaccctac ggctggcagc tccagcgcct ctgaagcgca gccgttttac
    18241 gttaactccc cgtatggcat tacgcttgcc cacaacggca atctgaccaa cgctcacgag
    18301 ttgcgtaaaa aactgtttga agaaaaacgc cgccacatca acaccacttc cgactcggaa
    18361 attctgctta atatcttcgc cagcgagctg gacaacttcc gccactaccc gctggaagcc
    18421 gacaatattt tcgctgccat tgctgccaca aaccgcttaa tccgcggcgc gtatgcctgt
    18481 gtggcgatga ttatcggcca cggtatggtt gctttccgcg atccaaacgg gattcgtccg
    18541 ctggtactgg gaaaacgtga tattgacgag aaccgtacag aatatatggt cgcttccgaa
    18601 agcgtagcgc tcgatacgct gggctttgat ttcctgcgtg acgtcgcgcc gggcgaagcg
    18661 atttacatca ctgaagaagg gcagttgttt acccgtcaat gtgctgacaa tccggtcagc
    18721 aatccgtgcc tgtttgagta tgtatacttt gcccgcccgg actcgtttat cgacaaaatt
    18781 tccgtttaca gcgcgcgtgt gaatatgggc acgaaactgg gcgagaaaat tgcccgcgaa
    18841 tgggaagatc tggatatcga cgtggtgatc ccgatcccag aaacctcgtg tgatatcgcg
    18901 ctggaaattg ctcgtattct gggcaaaccg taccgccagg gcttcgttaa aaaccgctat
    18961 gttggccgca cctttatcat gccgggccag cagctgcgtc gtaagtccgt gcgccgtaaa
    19021 ctgaatgcca accgcgccga gttccgcgat aaaaacgtcc tgctggtcga cgactccatc
    19081 gtccgtggca ccacttctga gcagattatc gagatggcac gcgaagccgg agcgaagaaa
    19141 gtgtacctcg cttctgcggc accggaaatt cgcttcccga acgtttatgg tattgatatg
    19201 ccgagcgcca cggaactgat cgctcacggt cgcgaagttg atgaaattcg ccagatcatc
    19261 ggtgctgacg ggttgatttt ccaggatctg aacgatctga tcgacgccgt tcgcgctgaa
    19321 aatccggata tccagcagtt tgaatgctcg gtgttcaacg gcgtctacgt caccaaagat
    19381 gttgatcagg gctacctcga tttcctcgat acgttacgta atgatgacgc caaagcagtg
    19441 caacgtcaga acgaagtgga aaatctcgaa atgcataacg aaggatgatg ccttcgctga
    19501 gggtgccggt ctggcaccct gacttgcaac tcccgccgaa atactgcaaa gtctgcctgc
    19561 aagtctgaca gggcaactat ttatgaaacg actcattgta ggcatcagcg gtgccagcgg
    19621 cgcgatttat ggcgtgcgct tattacaggt tctgcgcgat gtcacagata tcgaaacgca
    19681 tctggtgatg agccaggcgg cgcgccagac cttatccctc gaaacggatt tttccctgcg
    19741 cgaagtgcag gcattagctg atgtcacgct cgatgcgcgc gatatttccg ccagcatctc
    19801 ttccggttct ttccagacgc tggggatggt gattttaccc tgttcaatca aaaccctgtc
    19861 cggcattgtc catagctata ctgatggctt actgacccgt gcggcagatg tggtgctgaa
    19921 agagcgtcgc ccgttggtgc tctgcgtgcg tgaaacacca ttgcacttag gccatctgcg
    19981 tttaatgact caggcggcag aaatcggtgc ggtgattatg cctcccgttc cggcgtttta
    20041 tcatcgcccg caatcccttg atgatgtgat aaatcagacg gttaatcgtg ttcttgacca
    20101 gtttgcaata acccttcctg aagatctctt tgcccgctgg cagggcgcat aactccgctg
    20161 ttgccctgtt tcagggcaat tttgcaaccg cgatcaaatc ctcgacattt tgtttctgcc
    20221 attcaatcga aacgctgcga ttcaaccgct atacctgcta tcttcaactt caggacaata
    20281 atgcaatgtc ttattaacat atttaacgtt gaatgttact gttgtcgtca agatggcata
    20341 agacctgcat gaaagagcct gcaaacacac aacacaatac acaacataaa aaagccattt
    20401 tcacttgagg gttatgtatg aagaagtcga ttctcgctct gtctttatta gtcggtctct
    20461 ccgcagcggc ttccagctat gcggcgctac cggagacggt acgtatcgga accgatacca
    20521 cctatgcgct gttctcgtcg aaagatgcta aaggtgattt tgttggcttt gatatcgatc
    20581 tcggtaacga gatgtgcaaa cggatgcagg tgaaatgtac ctgggttgcc agtgactttg
    20641 acgcgctgat cccctcactg aaagcgaaaa aaatcgacgc tattatttcg tcgctttcca
    20701 ttaccgataa acgtcagcag gagattgcct tctccgacaa gctgtacgcc gcagattctc
    20761 gtttgattgc tgccaaaggt tcaccgattc agccaacgct ggattcactg aaaggtaaac
    20821 atgttggtgt gctgcaggga tcaacccagg aagcttacgc taacgagacc tggcgtagta
    20881 aaggcgtgga tgtggtggcc tatgccaacc aggatttggt ctattccgat ctggctgcag
    20941 gacgtctgga tgctgcgtta caagatgaag ttgctgccag cgaaggattc ctcaagcaac
    21001 ctgctggtaa agatttcgct tttgctggct catcagtaaa agacaaaaaa tacttcggtg
    21061 atggcaccgg tgtagggcta cgtaaagatt atgctgaact gacggctgcc ttcaataagg
    21121 cgcttggcga gctgcgtcag gacggcacct acgacaagat ggcgaaaaag tatttcgact
    21181 ttaatgtcta cggtgactga tacgtcgctg ggaagctgta cctgatggaa tgatcatcat
    21241 ggtgcacgcc aggtttgttg cactatcgtg gtgcattgaa atgcatactt aagcattttt
    21301 aatgaaaaat aatacgtcta acggggcggg atattttgcc ttgatggtca attttatggc
    21361 acgataagtg taacaaacct gtaaatattc cctataaaaa gactgtcagt tgaggacatt
    21421 atgaaaaaac tggtgctatc gctctctctg gttctggcct tctccagcgc aactgcggca
    21481 cttgctgcaa ttccgcaaaa catccgcatc ggtaccgacc cgacctatgc gccatttgaa
    21541 tcaaaaaatt cacaaggcga actggttggt ttcgatatcg agctggcgaa ggagttgtgc
    21601 aagcgcatca atacgcaatg tacgtttgtc gaaaacccgc tggatgcgtt aatcccgtcc
    21661 ttaaaagcga agaagattga cgccatcatg tcatcgcttt ccattacgga aaaacgtcag
    21721 caagaaattg ccttcaccga caaactgtac gctgccgatt ctcgtctggt ggtggcgaaa
    21781 aattctgaca ttcagccgac agtcgagtcg ctgaaaggca aacgggtagg cgtattgcag
    21841 gg
//
LOCUS       DAZSNM010000051        21811 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZSNM010000051 DAZSNM010000000
VERSION     DAZSNM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21811
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.051"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..963)
                     /locus_tag="ACHW42_001621"
     CDS             complement(<1..963)
                     /locus_tag="ACHW42_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR8943488.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFH"
     gene            1299..2501
                     /gene="nupC"
                     /locus_tag="ACHW42_001622"
     CDS             1299..2501
                     /gene="nupC"
                     /locus_tag="ACHW42_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR8943489.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(2551..4663)
                     /locus_tag="ACHW42_001623"
                     /pseudo
     CDS             complement(2551..4663)
                     /locus_tag="ACHW42_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(4871..4946)
                     /locus_tag="ACHW42_001624"
     tRNA            complement(4871..4946)
                     /locus_tag="ACHW42_001624"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4911..4913),aa:Ala,seq:ggc)
     gene            complement(4986..5061)
                     /locus_tag="ACHW42_001625"
     tRNA            complement(4986..5061)
                     /locus_tag="ACHW42_001625"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5026..5028),aa:Ala,seq:ggc)
     gene            5282..5641
                     /locus_tag="ACHW42_001626"
     CDS             5282..5641
                     /locus_tag="ACHW42_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR8943490.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            5643..6035
                     /locus_tag="ACHW42_001627"
     CDS             5643..6035
                     /locus_tag="ACHW42_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR8943491.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(6087..7502)
                     /gene="gltX"
                     /locus_tag="ACHW42_001628"
     CDS             complement(6087..7502)
                     /gene="gltX"
                     /locus_tag="ACHW42_001628"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR8943492.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            7761..7836
                     /locus_tag="ACHW42_001629"
     tRNA            7761..7836
                     /locus_tag="ACHW42_001629"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7794..7796,aa:Val,seq:tac)
     gene            7881..7956
                     /locus_tag="ACHW42_001630"
     tRNA            7881..7956
                     /locus_tag="ACHW42_001630"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7914..7916,aa:Val,seq:tac)
     gene            7961..8036
                     /locus_tag="ACHW42_001631"
     tRNA            7961..8036
                     /locus_tag="ACHW42_001631"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7994..7996,aa:Lys,seq:ttt)
     gene            8067..8192
                     /locus_tag="ACHW42_001632"
     ncRNA           8067..8192
                     /ncRNA_class="other"
                     /locus_tag="ACHW42_001632"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8312..8653
                     /locus_tag="ACHW42_001633"
     CDS             8312..8653
                     /locus_tag="ACHW42_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR8943493.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(8644..9570)
                     /locus_tag="ACHW42_001634"
     CDS             complement(8644..9570)
                     /locus_tag="ACHW42_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8943494.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            9660..10658
                     /locus_tag="ACHW42_001635"
     CDS             9660..10658
                     /locus_tag="ACHW42_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR8943495.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(10655..10873)
                     /locus_tag="ACHW42_001636"
     CDS             complement(10655..10873)
                     /locus_tag="ACHW42_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR8943496.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(10875..12890)
                     /gene="ligA"
                     /locus_tag="ACHW42_001637"
     CDS             complement(10875..12890)
                     /gene="ligA"
                     /locus_tag="ACHW42_001637"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR8943497.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(12961..13947)
                     /gene="zipA"
                     /locus_tag="ACHW42_001638"
     CDS             complement(12961..13947)
                     /gene="zipA"
                     /locus_tag="ACHW42_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR8943498.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            14177..14938
                     /gene="cysZ"
                     /locus_tag="ACHW42_001639"
     CDS             14177..14938
                     /gene="cysZ"
                     /locus_tag="ACHW42_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR8943499.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            15123..16094
                     /gene="cysK"
                     /locus_tag="ACHW42_001640"
     CDS             15123..16094
                     /gene="cysK"
                     /locus_tag="ACHW42_001640"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR8943500.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            16478..16735
                     /gene="ptsH"
                     /locus_tag="ACHW42_001641"
     CDS             16478..16735
                     /gene="ptsH"
                     /locus_tag="ACHW42_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR8943501.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            16780..18507
                     /gene="ptsI"
                     /locus_tag="ACHW42_001642"
     CDS             16780..18507
                     /gene="ptsI"
                     /locus_tag="ACHW42_001642"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR8943502.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            18548..19057
                     /gene="crr"
                     /locus_tag="ACHW42_001643"
     CDS             18548..19057
                     /gene="crr"
                     /locus_tag="ACHW42_001643"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR8943503.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(19099..19950)
                     /gene="pdxK"
                     /locus_tag="ACHW42_001644"
                     /gene_synonym="thiJ"
     CDS             complement(19099..19950)
                     /gene="pdxK"
                     /locus_tag="ACHW42_001644"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR8943504.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            20055..20429
                     /locus_tag="ACHW42_001645"
     CDS             20055..20429
                     /locus_tag="ACHW42_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR8943505.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            20462..21185
                     /locus_tag="ACHW42_001646"
                     /pseudo
     CDS             20462..21185
                     /locus_tag="ACHW42_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(21389..>21811)
                     /locus_tag="ACHW42_001647"
     CDS             complement(21389..>21811)
                     /locus_tag="ACHW42_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HFR8943506.1"
                     /translation="GRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIP
                     GIRRWPAEYLPGIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALR
                     VAKANPGAVVVAIICDRGDRYLSTGVFGEEHFSQGAGI"
BASE COUNT         5577 a         5604 c         5245 g         5385 t
ORIGIN      
        1 atggaagcga atgaatccct gcatcaccac ctgccccgcc agtgtcccca ccaccgttga
       61 ggacagtccg gcagcaacca gacttaaccc aaagaccgtt gccgcagcat ggcttaacag
      121 cggttgcagc gtcagataag cctcatcaag atcggcaaca ccagtatgac cagaaaagtg
      181 gaacgccgcc gcagctgtag ccatcatcac cagattgaca aaaccggcaa tcgtcatggc
      241 gatagccaca tcccatttgg tggcggaata acgttgttga cgcgaaccgc catgtaaatg
      301 ctgagtgagc gaagagtgca aataaatcac atgcggcata atcgtcgccc ctaacacgcc
      361 tgctgccagg aagaccgcct ccgaagtagg taaactcggg atcaccattc ctttacccag
      421 ctgcgccagg ttaggctggg agaaaatcaa ctcgacaatg taagccgcgg caacaaacaa
      481 cagtaacccg ccaatcactt tctccagcgg tttttgcccg cgacgttgca gcattaaaat
      541 caggaaagtc gcgatccccg tcagcaccgc gccctgcaac aacgaaacac caagaatgag
      601 tttaaaaccg atcgccgcac caataaattc cgccaggtcg gttgccatcg caataatttc
      661 tgcctgaacc caatagaacc acactacggg acgcggatag tgatcacgaa tctgctccgc
      721 cagattttta ccggtggcaa tccctagttt ggcagagagg atctgaatca gcatcgccat
      781 caggttggcc caaacgacaa cccacagtag ctgatagccg aagctagcac ccgcctgaat
      841 attggtcgca aagttaccgg gatcgatata accaatcgcc gcaatgaacg caggtcccat
      901 taatgcgagc ctcatcttac gcgccgcccg tccgctgcta ctctcaacgc gatagttcgt
      961 catcttgtgc ctctaaaaca tagcctttgc tatgtttcat gctatgctaa acgagaatga
     1021 ttatcaaatt catttaaatg gattgtggtg atttctctga tagaccaaga ttatgattac
     1081 gaaaagattg atgacttgaa tgttatggtt atgtttaatg ttagcacatt tacataactt
     1141 tcagcttcca tacacaacat agcagaaatg tatgacagat cactattttt gaagcctgtc
     1201 acaggacgtc attatagtgt gtgtcagatc tcgttttcct taaccatgtt acatagaatg
     1261 tgcacggaaa tttaacctgc ctcatatttg gagcaaatat ggaccgcgtc cttcattttg
     1321 tactggcact tgccgttgtt gtgattctcg cactgctggt aagcagcgac cgcaaaaaaa
     1381 ttcgtatccg ttatgttatt caactgcttg ttatcgaagt gttactggcg tggttcttcc
     1441 tgaactccga cgttggttta ggcttcgtga aaggcttctc cgaaatgttc gaaaaactgc
     1501 tcggatttgc caacgaaggg actaacttcg tctttggtag catgaatgat caaggcctgg
     1561 cattcttctt cctgaaagtg ctgtgcccaa tcgtctttat ctctgcactg atcggtattc
     1621 tccagcacat tcgcgtgttg ccggtgatta tccgcgcaat tggtttcctg ctctccaaag
     1681 tcaacggtat gggcaaactg gaatccttta acgccgtcag ctccctgatt ctgggtcagt
     1741 cagaaaactt tattgcctat aaagatatcc tcggcaaaat ctcccgtaat cgtatgtaca
     1801 ccatggctgc cacggcaatg tccaccgtgt cgatgtccat cgttggtgca tacatgacca
     1861 tgctggaacc gaaatacgtc gttgctgcgc tggtactgaa catgttcagc acctttatcg
     1921 tgctgtcgct gatcaatcct taccgtgttg atgccagtga agaaaacatc cagatgtcca
     1981 acctgcacga aggtcagagc ttcttcgaaa tgctgggtga atacattctg gcaggtttca
     2041 aagttgccat tatcgttgcc gcgatgctga tcggctttat cgccctgatc gccgcgctga
     2101 acgcactgtt tgccaccgtt actggctggt ttggctacag catctccttc cagggcatcc
     2161 tgggctacat cttctatccg attgcatggg tgatgggtgt tccttccagt gaagcactgc
     2221 aagtgggcag tatcatggcg accaaactgg tttccaacga gttcgttgcg atgatggatc
     2281 tgcagaaaat tgcttccacg ctctctccgc gtgctgaagg catcatctct gtgttcctag
     2341 tttccttcgc taacttctct tcaatcggga ttatcgcagg tgcagttaaa ggcctgaatg
     2401 aagagcaagg taacgtggtt tctcgcttcg gtctgaaact ggtttacggc tctaccctgg
     2461 tgagtgtgct gtctgcgtca atcgcagcac tggtgctgta aaaccataca taaaaaagcc
     2521 ggggatcatt ccccggcttt tttacgcctg ttaatcagct aatggctgcg ggcgaccaat
     2581 caaataccct tgcagatatt gcaccccgag cttatgcaat agcgcctgct gctgttgcgt
     2641 ctcgacaaac tccgcgacca cactcaatga cttcgctttc gccagatcgg taattgatct
     2701 cacaatcatc gcatccagcg tattcgtgac aatatctttc acaaagacgc catcaatttt
     2761 gatgatatca gcctgcaaac gctttaaccg ttcgtagttg gcatatccgg tgccaaagtc
     2821 atcaatcgca atccggaaac caaacttatg cagctgctcg atgttgtaca tgctgctttc
     2881 tgcgttagaa aacgcctgct cctcggtgat ctcaagaatg accgcctgcg gggagatgtg
     2941 ataacgctta aacagacgaa taatccgccc ggcaatattc ttttgcagca gcgtgagcgg
     3001 cattaaattg actgaaaagc gcggtccttt tttgtcgcaa gggtgtgttg ccagccactt
     3061 caacaaggat tccagcactt gcaaatcaaa acgcgcgcta aggttaaact gagcaataag
     3121 gggcagaaac ttatccgggg tcataatgcc gccgtcatat ttcagtcgcg cgaggatctc
     3181 atcataacct tcaccctctt tgttgcgaat tggctgggcg tagagcagca aatcaccctg
     3241 atctaacgcc gtgcgaatgg tattcagcaa tagcacctgt ttagtggtct gcccggaaac
     3301 catctcctct ctgctatcca gcgccagcac atgatgatgt gcgcaggatt gctccgccag
     3361 ccagcttaac tgccccaaca agggttgcag ggtttcctga tttccatcaa aacgccccca
     3421 ggcagcacca tagcccatat ccagcccggt attgttccag tgaatttgcc gactattcag
     3481 gatgttaacc atatgctgga gtcgcccttc cgtttccggc ccgctcagca ctaacagcag
     3541 ttcactaccc ggcaattgat acaacttttc gttttcctgc atcaacggca gcagcgtacg
     3601 gcaaattgag cggatacagt gaacgcgcat cattaagccg taatgacgac tcataaactc
     3661 aagattatca atgcgcaggc agcaaaaact cttgcccgcc tcttgctccg gcgcttgttc
     3721 caacgcacga aagttgggta ggagcgttaa cggatctgtt agcgcctgca aatgccagcg
     3781 gcgattaagc cattcactgc gatgataaat gcgcaccata tagagcaggc aaacgctaaa
     3841 ggaaatcagc accgcgagaa taaatgccag cgaatattcg gtttccaccc cttgcaaaaa
     3901 gttctgattg taattcagaa ggcaaagcgt tgaaacagcc caggtgagat ttaaaaacgg
     3961 atagcgaaga ttaccgaccc cgagggtaaa gatgatgaag aaaacgggta ccaggtaacc
     4021 ggcaataaag tcgttttcat aaggtgtgca caacagaagt agcagcacgc ttaacgctgc
     4081 cagccagctt aaggtaaatg cgagtgagat agtagaaaag catgttgtaa atcagcactg
     4141 cggtgaaaag gcttagtaaa tcaacgaccg tgaaaatggc atccgcatcg ccgaaaaagg
     4201 tggatatctt tagcgggaaa tcaaagaagc tccccacaag atacatgctg catttgatgc
     4261 cgatcggcgt caccaaacca agccagaaca gacgttgcca gacatatcgg ctggtcaatc
     4321 cataacgcca acgcgtcccc agctgccagc gtaatattgc gcaggcacac aacacgacaa
     4381 acgtctggct gccgagcatg accgcagtct gtaaaaaaga gagatgaaag ttccacagat
     4441 tggtgcaaaa catcccgcat aaaacgggaa cgacaccgcg ccagccaaag ataaacaaga
     4501 ttgacagcat tacgcacagt ggcatccacg ccagaaagat ataactggaa tggataatgg
     4561 caagtggcga aataaaacgg gatagctgaa taagtaccac ggtgagcgta aacgctagtg
     4621 tgaatatctt gatatttttt atcaggttat gctccacaaa catgagtaca ctactctcac
     4681 tgatcggggt tttcaggcac acaaataata agtttattcg catcgcgaag caaacagggg
     4741 ccagcctgtt gccgattttt catccaatga tgctttcacc aggcggcgct aacccggacg
     4801 tagactgtat tacaaaagcg gcaaaaagca gagacaaaaa acccccgctt tgcagcgagg
     4861 gttggaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat gccatgcaag
     4921 cgctctccca gctgagctat agccccacga tgcgtttacg taccaaattt gctgggtgca
     4981 aaatttggtg gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc
     5041 tcccagctga gctatagccc cgtcacgtaa agcttgtcga gttgacgggc ggcatcatat
     5101 gaattccgcc cgaatgtgtc aacggcaaat tgcaacgtgt agtttcaatc gctgaaaaat
     5161 caggcaaatg aacaattttg gaaaaccgct cgcattcagt agttattcat gtcacggttt
     5221 cctgtaaagt ggtgttataa aatgaactac taatagaccc acatacattc agggaattgt
     5281 tatgttcaag gagaggatga caccagatga acttgccaga ctgaccggct atagccgcca
     5341 gaccattaat aaatgggtac gcaaggaagg ctggacaacg tcaccaaaac ctggcgtcca
     5401 gggtggcaaa gcccgactgg ttcacgtcaa tgaacaggtt cgtgaatata ttcgcaatgc
     5461 tgaacgtcca gaaggtcagg gagaagcgcc tgccctttct ggtgatgcgc cgcttgaagt
     5521 attgctggtg acactggcga aagagatgac gccagttgaa caaaaacagt ttacatcctt
     5581 gcttctgcgg gaagggatta tcggattgtt acaacgctta gggattcgcg atagcaaata
     5641 atatgaaaag attacgcaat aaaatgacca ccgaagaact ggctgaatgc ctcggtgtgg
     5701 ctaaacaaac tgttaaccgt tggaccagag aaaaaggctg gaaaacggaa aaatttcccg
     5761 gcgtgaaagg cggtcgtgcc agacttattc tggtcgatac gcaagtttgc gagtttattc
     5821 agaacacgcc agccttccat aacacaccaa tgttgatgga agctgaagag agaattgctg
     5881 aatacgctcc tggagcacgc gccccggcgt atcgacaaat catcaacgcg atagataata
     5941 tgacagacat tgagcaggaa aaagtcgcgc aatttttgtc acgtgaagga attcgcaatt
     6001 tcctcgcccg tctcgatatt gacgaatcag cataaataaa aaacggcagg atattatctc
     6061 ctgccgttta tctttttaca cgctaattac tgctgatttt cgcgttcagc aataaaatcc
     6121 agcgctttgt tgatacgctc gatactgcgg gtcttaccaa ttgcgtgaac ggtaacatcc
     6181 agtgctggag actgccccgc accggttacg gcgacacgca gcggcatacc aactttaccc
     6241 atacccactt ccagctcatc cgccgtcgcc tgaatagcgt gatgaacgtt ttcagcggtc
     6301 cagtcagtaa tcgcggccag tttgtcacga accacttcca gcggctgacg cgctaccgga
     6361 cgcagatgtt ttttcgccgc atcggcatcg aactcagcaa aatcttcgta gaaataacgg
     6421 cagctctgtg ccatctcttt cagcgtcttg cagcgttcgc ccagcagttt caccagatca
     6481 gccagctgcg ggccgttacg ggtatcgata ttttcctgct caatgtgcca ctgtaagtga
     6541 gtagcaacat actccggcgg cagcgcgtta atgtagtgat ggttcagcca cagcagcttg
     6601 tcggtgttga acgcactggc agatttgctg acggcattca aagtgaagta tttgatcatc
     6661 tcttcacgag tgaagatttc ctgatcgccg tgggaccagc ccagacgcac cagatagttc
     6721 agcagtgctt ctggcaaata accgtcatca cgatactgca ttacgctgac tgccccgtga
     6781 cgtttggaca gttttttacc gtcatcgcca ttgatcatag aaacgtgcgc gtaaaccggc
     6841 accggcgctt tcagggcctt aagaatgttg atctggcgcg gcgtgttgtt gatatggtct
     6901 tcgccacgga taacgtgggt gatttccata tcccagtcat cgacaaccac acagaagtta
     6961 taggttgggg aaccatcggt acggcggata ataagatcgt ccagttcctg gttgctgaac
     7021 tcgatcggac cacggatctg atcgtcaaaa acaacagaac cttcctgcgg gttagcaaaa
     7081 cgtacaacac acggttcatc atcagcatga tgctcatggc tgtggcggca gcgaccgtca
     7141 taacgcggct tctcaccttt cgccatttgc tcttcgcgca gcgcttccag gcgctcttta
     7201 gagcaatagc atttataagc agtgccctct tccagcatct gatcgatcac cgcgttgtag
     7261 cgatcaaaac gtttggtctg gtagtacgga ccttcatccc actccaggct cagccagttc
     7321 atgccatcca taatggcttc gatagcttcc ggcgtggaac gctcaagatc ggtgtcttca
     7381 atacgcagca cgaactcacc gccgtggtta cgtgcaaaaa gccaggagta aagagcagta
     7441 cgcgcgccgc caacgtgcag atagcctgtt gggcttggcg cgaagcgagt tttgattttc
     7501 atgaaatggc cttacgttta gaaagatgcc gacaaccggc aaatcctgga aaaattgagt
     7561 gggcgatatt ctatcactcc cgcctgattc ctcaatgtag ttcgggtttc taccttacgc
     7621 attattgttt tcgttgacaa attgcgcaac acggtctgtt ttgcgatcat cttgcttaaa
     7681 tttacgacga acgaacaatt tctttagaaa atgcgttgac tcattttgaa ctctccctat
     7741 aatgcgactc cacacagcgg gggtgattag ctcagctggg agagcacctc ccttacaagg
     7801 agggggtcgg cggttcgatc ccgtcatcac ccaccaacta ctttatgtag tctccgccgt
     7861 gtagcaagaa attgagaagt gggtgattag ctcagctggg agagcacctc ccttacaagg
     7921 agggggtcgg cggttcgatc ccgtcatcac ccaccacttc gggtcgttag ctcagttggt
     7981 agagcagttg acttttaatc aattggtcgc aggttcgaat cctgcacgac ccaccaatgt
     8041 aaaaaagcgc cctaaaggcg cttttttgct atctgcgata ctcaaagatt cgaacctgca
     8101 gcaggtttga gttgagcgca gcgaaacaac ggagccgctc gcggcgacgg cccgaagggc
     8161 gagcgaagcg agtcatcctg cacgacccac caatgcaaaa aagcgcccta aaggcgcttt
     8221 tttgctatct gcgatttgta agattgattt ttcttctcaa gttccttgtt cttctaccga
     8281 tattacctcg tacagtgacg aggactgaac aatggccact atctcttcaa cttcaattcc
     8341 ttccatacaa acacagagta gtaacagggc atcacaaggt agcgatgttg cttcacaaat
     8401 cgcgcggata agtcagcaaa tcatcaaact cactcaacaa attaaagaga ttgtagacac
     8461 tagcggcagt gctgaagaca agcaaaaaca ggccgaattg attcaacagc aaatcaccct
     8521 actggaaaca caactggcgc agttgcagaa acagcaggca gaaaaggcgc aggagaaaga
     8581 gcagcgtttg tctctgaatg tatcccttct aaatcctgtt gaaaaaacga ctcatatcga
     8641 tatctatatc tgatacaacg gatccccttc ccgaccagcc attagcgcat tgccctggtc
     8701 tcgtaccacg tcccataacg cttcagcggc ggtggaaagt gaacggttct tcctgcgcac
     8761 cagcatcaac tggcgttcaa ccaccggcgt aatgcgctta accaccagtg ggctaccttc
     8821 aggcaatggt agcgccagtg caggaagaat actgatgccg atgcctgcgg ctaccatcgg
     8881 gaacagcgtc gccggatgtc caatctcctg cacaatattc gcctgaatac cattgcgcgc
     8941 cagcgctgcg tcaatcagcg gtcggctgcc tgacgcgtaa tcctgcaaca ccaatttcgc
     9001 tccctgtaat gcctgccagg gtacataatc ttccacggcc aaagcgctat cgcgatggca
     9061 aagcagaaag aaaggctcgg aaagaatcgc ttcacattgc agatcgccca ctgggcctgg
     9121 atcgatgacg atgccaaagt cgacatctcc ctggcgaata ctttccatca cccactgctg
     9181 cggacgatca tgcaggacaa actgaatatc tggatagcgg cgatggcttt cagcaatgca
     9241 ttgcggaata agatgcgcgg aaatggtctg gctggcagcg acccgaactt ttccgctcag
     9301 ttgttgcccc atacgcccgg tatcgcgcaa cgtgctgtta agttcatcca gcagtcgctc
     9361 aagacgcaaa gccaactgct gccctgcatc tgtaagcacc acttcacgcg tggttctgtc
     9421 cagcaggcga acaccagtat gattttccag ctccttcaca ctgtgactta ctgccgactg
     9481 gctcaagccg atacgctctc ctgcacgact aaaacttttc tcctgcgcta ctgtgacgaa
     9541 aacctttaat tgttttaaag aataattcat ctgttttctt catgaatgga tgcaataaat
     9601 caattttatt tctcaaactg gaagaagcac aatagaacca tcgatcatct ggagtcttta
     9661 tgaaactttt tcgtatcctc gatcctttca ccttaaccct gatcacggtg gtgttgctgg
     9721 cctctttctt tccggccaga ggcgatttcg tccccttctt tgaaaatctg accaccgcag
     9781 ctattgccct gctgttcttt atgcacggcg cgaagttgtc gcgtgaggcg attattgctg
     9841 gcggtggtca ctggcgactg catttgtggg taatgtgcag caccttcgtg ctgtttccga
     9901 ttctgggtgt actgtttgcc tggtggaaac cggtaaatgt cgacccgatg ctctactccg
     9961 gttttctcta cttgtgcatt ctcccggcta ccgtgcagtc tgcaatcgcc ttcacgtcaa
    10021 tggcgggcgg taacgtcgcg gcggcggttt gttctgcgtc agcatccagc ctgctgggga
    10081 ttttcctttc accattgctg gttggtctgg tgatgaatgt tcacggtgca aggggcagcc
    10141 ttgagcaggt cggtaaaatt atgctgcaac tgctgctgcc gtttgtgttg gggcatcttt
    10201 cccggccgtg gattggtgac tgggtgtcgc gcaataaaaa atggattgcg aaaactgacc
    10261 agacgtccat tctgttggtg gtttatacag cgttcagcga agccgtcgtt aatggtatct
    10321 ggcataaagt tggctgggga tcattgctgt ttatcgtggt ggtcagctgc gttcttctgg
    10381 ctatcgtgat tgtagttaac gtctttatgg cacgccgact gagcttcaat aaggcagatg
    10441 aaattactat cgtcttttgt ggttcgaaaa agagtctggc aaatggcatc ccgatggcaa
    10501 acattctgtt ccccacatcg gtgatcggta tgatggtgct gcccctgatg attttccatc
    10561 agatccaatt gatggtctgt gcggtgctgg cgcgtcgata caaacgccag accgaacagt
    10621 tacaggcgca gcaggaaagc agcgccgata aagcttaaag cggacgcttc aggggctgga
    10681 ccagttgcgt cagcccctcg gttttaatca gcagcgtgat ttgcattagc tcaccgagct
    10741 tccctgccgg gaactcatct ttgcgggcaa accacagcag atactcctcc ggcaggtcaa
    10801 ttaagcgacg ccctttgtat ttgccaaacg gcattatcgt attggctatt tcaatcagct
    10861 gctctttttc catctcagct acccagcaaa cgcagcattt ccgcttcgtc gatgacttca
    10921 atgcccagtt cctgcgcctt cgccagttta gatcctgcag cttcacccgc tatcaccaga
    10981 tcggttttct tcgacacgct gcccgcgact ttcgcgccca gttcgaccag tcgagcttta
    11041 gcgtcatcac gcgacatctg gcttaagctg cccgtaagca ccacggtttt accagcaaac
    11101 gggctgtcaa tctcttccgc gttgataacg atcggcgcag gccagtgaac accttccgcc
    11161 aacagctcgc tgatgacatt gcggttgctt tcttcggcaa agaagttgtg aacgtgggat
    11221 gcaacgacaa tgccaacatc aggcaccttt tgcagctctt caatcgaagc ggcttccagc
    11281 gcttccagcg tgccgaaata tgccgccaga cctgctgcgg tggcctcgcc gacttcacgg
    11341 atgccaagtg catagaggaa gcgagcaaag gtggtttctt tcgctttttc cagcgcgtta
    11401 accacgtttt gtgccaattt tggccccata cgctccagtc cggtcagttt gcctgcggtg
    11461 agtttgaaca gatctgccgg agtatggaca tattcttttt caaccagctg atcgatgatt
    11521 ttgtcgccca ttccgtcaac atccatcgca cggcgggaaa caaagtgttt cagcgactct
    11581 ttacgctgcg caccgcaaat caggccaccg gtacagcggg caaccgcttc accttccaca
    11641 cgctcaacgt cagaaccaca taccggacaa tgcgtcggga atacaacctc acgggtatct
    11701 tccgggcgtt cagaaagcac gacgttaacc acctgcggga tcacgtcgcc agcgcggcga
    11761 atcaccactt tatcgccaat gcgtaaacca agacgttcga tttcatccgc attgtgtaag
    11821 gttgcgttac tcaccagcac gccagcaaca tggacaggtt ccagacgcgc aacaggcgta
    11881 atcgcgccag tacgcccaac ctgaaactcg acgtcacgca caaaggtcat ctgctcctgc
    11941 gccgggaatt taaacgcaac cgcccagcgc ggggcacgcg cgacaaagcc aagctgctcc
    12001 tgctgttcca gtgagttgac cttaatcacc acgccgtcga tatcaaagcc cagcgtcggg
    12061 cggtcttctt ccactttgtg atagaacgcc agcacttctt ccgccgattc acaaagcgtt
    12121 acccgatcgc tgaccggcac cccccacttt ttaaattgca gtaaacggcc aagatgagta
    12181 tccggcagct cgccaccttc cagaacacca acgccatagc agaaaaaagt gagcggtcgc
    12241 ttcgctgtaa tacgcggatc aagctgacgc agtgaaccag ctgccgcatt acgtgggtta
    12301 gcaaaaactt tcccgcccgt gcgtcgcgca tcttcgttaa tcttttcgaa ccccgcctgc
    12361 ggcaggaaca cttcaccacg cacttccaga cgcgccggga tattctctcc gtgcagcttc
    12421 agcggaatgg cgcgaatagt acgcacatta gacgtgatat cttccccggt ggtgccatcg
    12481 ccacgggtcg cggcactgac taaaacgcca ttttcataca gaatactgac ggcaaggcca
    12541 tccagcttca gctcacagca ccaggtgact ttctcgttgt ttttcagacg gtcctgcaca
    12601 cgtttgttga aagcaagaaa gctttcttca tcaaaaacgt tatccagtga cagcattggt
    12661 acttcatggc gtatctggct gaaagctgcc agcggcgcag cacctacacg ttgggtaggc
    12721 gaatcaggcg taatcagttc tggatgtttg gtttccagct cgcgcagttc gcgcatcagc
    12781 ctgtcgtatt cagcgtcggg aatttccggc gcatccatca catgataaag atattcatga
    12841 tggcaaagcg tcgttcgcag ttctgtcagt tgttgttcga ttgattccat atcgcaccat
    12901 caatgctaaa aacccccgac aagcgggggt tcgaagagga gttaatttgc cttaagtgta
    12961 tcaggcgttg gcgtctttga cttcgcggat gatgtcctgg tactcgcgca atttctgcgg
    13021 agtcatcata cggcgctgat cgtcaagcac gacaccgccc acttcatcgg caatatgctg
    13081 cgcagattgc agcatcagct tgaagttctg cagctcgtca ccgtaagacg gtacctgcat
    13141 aaagatagtg acacccggag tagtgaaatc cttcatttca ggatcaaagg ttcccggttt
    13201 caccatattc gccaggctga ataacgccgg gccgctgcca tccgggctaa gatgacgatg
    13261 gtaaatattc atatcgccaa aaatgaagcc cgcttgttga atgctgttaa gaagcagttc
    13321 accgtttagc tcgctaccgt gatgcgccgc gacgttcatg ataatcaccg cttctttgcg
    13381 cttcggttta tccataactg gagccggttc cgctacaggc tcaggctgtg gtgccgctac
    13441 gggttctgca ggctggaaag cctgttgtgc cggttgcggt gctgaatgca caggctgcgg
    13501 cgctggcgcg acctgtggcg aaactggctg ctgcaacggc tgttcaggct gcggctgata
    13561 ggcaggctgc tgcaccggct gcgctggacg cggagcatgt tgcggcggta cctgcgcttc
    13621 aggcggctgc tggaccggtt gacgcggctg cgcagacgca taaggcggta ggtactggtg
    13681 ttgcggcgac ggacgagcag cctcatgctc ctgagcgtta gccggggcat gattcacgcg
    13741 gtgaacacga acctcaccaa cgccctcatc atcttcgaca tcctcgtcat aagaatcgtc
    13801 gtcacgtttt gacttcattc gttttaatgg ccgatcgcgg aacatagaag atcgttcttt
    13861 acggctggtc cagaaaccat gtaccagtaa agcgattatg gcgatcgcgc caacaatgat
    13921 taatatcaga cgcaaatcct gcatcattat attctctgtt gttctaacac cttgccacca
    13981 cggcaaacat ttactcacta agagtatttg ccgattacct caagtgcaag tgcactatta
    14041 actttcacag cacaaagata gatgaaatcg tgctttttgc tgttttttcg aacatatcct
    14101 aactgtccat tgcgcaatta cccggtaaaa tacgcagaat tttcctggga ttggtcaaaa
    14161 ggagctcatc ctgattatgg tttcatcatt cacatctgcc ccacgcagcg gtttttacta
    14221 ttttgcgcaa ggctggaagc tcgtctcgca acctgggatt cggcgtttcg ttattttacc
    14281 gctgctggtc aatattttgt tgatgggggg cgcattctgg tggctcttta cacagctcga
    14341 tgtctggatc ccgactttca tgagttacgt tccggactgg ctacaatggc tgagttatct
    14401 gttgtggcct ctggctgtca tctctgtgct gttagtgttt ggctatttct tctccacgat
    14461 tgctaactgg attgccgctc cgtttaacgg tttattggct gaacaactgg aagcacgatt
    14521 gactggcgct acaccgccag ataccgggat tttcggtatc atgaaagatg tgccgcgaat
    14581 catgaaacgc gaatggcaaa aatttgtctg gtatctgccg cgcgcaattg tattactaat
    14641 tctttacttc atccctggta ttgggcaaac cgtcgcgccg gtactgtggt tcctgtttag
    14701 cgcctggatg ttagccatcc agtattgcga ttaccccttc gataaccaca aagtgccgtt
    14761 taaagagatg cgcaccgccc tgcgcacacg caaaatcacc aatatgcagt ttggtgcttt
    14821 aaccagcctg tttacgatga tcccgctgct taatctgttc atcatgcccg ttgccgtttg
    14881 tggcgcgacg gcgatgtggg tcgattgcta tcgcgataaa cacgcgatgt ggcggtaaca
    14941 atctaccggt tattttgtaa accgtttgtg tgaaacaggg gtggcttatg ccgcccctta
    15001 ttccatcttg catgtcatta tttcccttct gtatatagat atgctaaatc cttacttccg
    15061 catattctct gagcgggtat gctacctgtt gtatcccaat ttcatacagt taaggacagg
    15121 ccatgagtaa gatttttgaa gataactcgc tgactatcgg tcacacgccg ctggttcgcc
    15181 tgaatcgcat cggtaacgga cgcattctgg cgaaggtgga atctcgtaac cccagcttca
    15241 gcgttaagtg ccgtatcggt gccaacatga tttgggatgc cgaaaagcgc ggcgtgctga
    15301 aaccaggcgt tgaactggtt gaaccgacca gcggtaatac cgggattgca ctggcctatg
    15361 tagctgccgc tcgcggttac aaactcaccc tgaccatgcc agaaaccatg agtattgaac
    15421 gccgcaagct gctgaaagcg ttaggtgcaa acctggtgct gacggaaggt gctaaaggca
    15481 tgaaaggcgc aatccaaaaa gcagaagaaa ttgtcgccag caatccagag aaatacctgc
    15541 tgctgcaaca attcagcaat ccggcaaacc ctgaaattca cgaaaaaacc accggtccgg
    15601 agatctggga agataccgac ggtcaggttg atgtatttat tgctggcgtt gggactggcg
    15661 gtacgctgac tggcgtcagc cgctacatta aaggcaccaa aggcaagacc gatcttatct
    15721 ctgtcgccgt tgagccaacc gattctccag ttatcgccca ggtgctggca ggtgaagaga
    15781 ttaaacctgg cccgcataaa attcagggta ttggcgctgg ttttatcccg gctaacctcg
    15841 atctcaagct ggtcgataaa gtcattggca tcaccaatga agaagccatt tctaccgccc
    15901 gtcgtctaat ggaagaagaa ggtattcttg caggtatctc ttctggagca gctgttgccg
    15961 cagcgttgaa actacaagaa gatgaaagct ttaccaacaa gaatattgtg gttattctac
    16021 catcatcggg tgagcgttat ttaagcaccg cattgtttgc cgatctcttc actgagaaag
    16081 aattgcaaca gtaatgccag cttgttaaaa atgcgtaaaa aagcaccttt ttaggtgctt
    16141 ttttgtggcc tgcttcaaac tttcgcccct cctggcattg attcagcctg tcggaactgg
    16201 tatttaacca gactaattat tttgatgcgc gaaattaatc gttacaggaa aagccaaagc
    16261 tgaatcgatt ttatgatttg gttcaattct tcctttagcg gcataatgtt taatgacgta
    16321 cgaaacgtca gcggtcaaca cccgccagca atggactgta ttgcgctctt cgtgcgtcgc
    16381 gtctgttaaa aactggcgct aacaatacag gctaaagtcg aaccgccagg ctagacttta
    16441 gttccacaac actaaaccta taagttgggg aaatacaatg ttccagcaag aagttaccat
    16501 taccgctccg aacggtctgc acacccgccc tgctgcccag tttgtaaaag aagctaaggg
    16561 cttcacttct gaaattactg tgacttccaa cggcaaaagc gccagcgcga aaagcctgtt
    16621 taaactgcag actctgggcc tgactcaagg taccgttgtg actatctccg cagaaggcga
    16681 agacgagcag aaagcggttg aacatctggt taaactgatg gcggaactcg agtaatttcc
    16741 cgggttcttt taaaaatcag tcacaagtaa ggtagggtta tgatttcagg cattttagca
    16801 tccccgggta tcgctttcgg taaagctctg cttctgaaag aagacgaaat tgtcattgac
    16861 cggaaaaaaa tttctgccga ccaggttgat caggaagttg aacgttttct gagcggtcgt
    16921 gccaaggcat cagcccagct ggaaacgatc aaaacgaaag ctggtgaaac gttcggtgaa
    16981 gaaaaagaag ccatctttga agggcatatt atgctgctcg aagatgagga gctggagcag
    17041 gaaatcatag ccctgattaa agataaacac atgacagctg acgcagctgc tcatgaagtt
    17101 atcgaaggtc aggcttctgc cctggaagag ctggatgatg aatacctgaa agaacgtgcg
    17161 gctgacgtac gtgatatcgg taagcgcctg ctgcgcaaca tcctgggcct gaagattatc
    17221 gacctgagcg ccattcagga tgaagtcatt ctggttgccg ctgacctgac gccgtccgaa
    17281 accgcacagc tgaacctgaa gaaggtgctg ggtttcatca ccgacgcggg tggccgtact
    17341 tcccacacct ctatcatggc gcgttctctg gaactgcctg ctatcgtggg taccggtagc
    17401 gtcacctctc aggtgaaaaa tgacgactat ctgattctgg atgccgtaaa taatcaggtt
    17461 tacgtcaatc caaccaacga agttattgat aaaatgcgcg ctgttcagga gcaagtggct
    17521 tctgaaaaag cagagcttgc taaactgaaa gatctgccag ctattacgct ggacggtcac
    17581 caggtagaag tatgcgctaa cattggtacg gttcgtgacg ttgaaggtgc agagcgtaac
    17641 ggcgctgaag gcgttggtct gtatcgtact gagttcctgt tcatggaccg cgacgcgctg
    17701 cccactgaag aagaacagtt tgctgcttac aaagcagtgg ctgaagcgtg tggctcgcag
    17761 gcggttatcg ttcgtaccat ggacatcggc ggcgacaaag agctgccata catgaacttc
    17821 ccgaaagaag agaacccgtt cctcggctgg cgcgctatcc gtatcgcgat ggatcgtaaa
    17881 gagatcctgc gcgatcagct ccgcgctatc ctgcgtgcct cggctttcgg taaattgcgc
    17941 attatgttcc cgatgatcat ctctgttgaa gaagtgcgtg cactgcgcaa agagatcgaa
    18001 atctacaaac aggaactgcg cgacgaaggt aaagcgtttg acgagtcaat tgaaatcggc
    18061 gtaatggtgg aaacaccggc tgccgcaaca attgcacgtc atttagccaa agaagttgat
    18121 ttctttagta tcggcaccaa tgatttaacg cagtacactc tggcagttga ccgtggtaat
    18181 gatatgattt cacaccttta ccagccaatg tcaccgtccg tgctgaactt gatcaagcaa
    18241 gttattgatg cttctcatgc tgaaggcaaa tggactggca tgtgtggtga gcttgctggc
    18301 gatgaacgtg ctacacttct gttgctgggg atgggtctgg acgaattctc tatgagcgcc
    18361 atttctatcc cgcgcattaa gaagattatc cgtaacacga acttcgaaga tgcgaaggtg
    18421 ttagcagagc aggctcttgc tcaaccgaca acggacgagt taatgacgct ggttaacaag
    18481 ttcattgaag aaaaaacaat ctgctaatcc acgagatgcg gcccaattta ctgcttagga
    18541 gaagatcatg ggtttgttcg ataaactgaa atctctggtt tccgacgaca aaaaggatac
    18601 cggaactatt gagatcattg ctccgctctc tggcgagatc gtcaatatcg aagacgtgcc
    18661 ggatgtcgtt tttgcggaaa aaatcgttgg tgatggtatt gctatcaaac caacgggtaa
    18721 caaaatggtc gcgcctgtag acggtaccat tggtaaaatc tttgaaacca atcacgcgtt
    18781 ctctatcgaa tctgatagcg gcgttgaact gttcgtccac ttcggtatcg acaccgttga
    18841 actgaaaggc gaaggcttca agcgtattgc tgaagaaggt cagcgcgtga aagttggcga
    18901 tactgtcatt gaatttgatc tgccgctgct ggaagagaaa gccaagtcta ccctgactcc
    18961 ggttgttatc tccaacatgg acgaaatcaa agaactgatc aaactgtccg gtagcgtaac
    19021 cgtgggtgaa accccggtta tccgcatcaa gaagtaattc ttgccgcagt gaaaaatggc
    19081 gcccatcggc gccatttttt atgcttccgc cagcggcggc aaaatcaatt catcgctctc
    19141 atgctgctgg gtgtagcgca tcacttccag tacgcgcaac cccgcacggt gcaccgcatc
    19201 gtttaacgcc ttccctttca gcaaaccact gataagctga gcacaaaaca ggtcgccagt
    19261 ccctttcagg tcggttttta cccgtgaatg ggaaatgaca ttcacgctgt cggcactgac
    19321 caccaccacc tgcatctcct gattttcttc attaccggag gcgctggtaa tcaccaccca
    19381 ttttaatgtg tctgaaagca gactttttgc ggcagcaatg gcactgtcga gatcgcggca
    19441 atttttaccg gtcaggattt ccagctcaaa gatattgggg gtaattccct gcgccagcgg
    19501 cagtaaatat tgtcgatacg cctcgggaag gtcaggtttg acataaattc cgctatcaat
    19561 atcgccaatc accggatcga ccatgatcaa taggtcagga tggtctttgc gtagcgcagt
    19621 cagccactcg gcaaggattt tgatttgcga tgccgttccc atatagcccg tggttacagc
    19681 acgaagttgg cgcagcgcat cacgctcctg aagcgcacgc aaatagccgc taaaccattc
    19741 gtccggaatc gcaccaccgt agaaagtgtc ataatgcggc gtattgctca gcaataccgt
    19801 cggcacggca aagacattca agccgttctg ttttatagca ggcaccgcaa tgctgttgcc
    19861 tacgcttccg tacactacct gcgactgcac ggcgacgata tccgcctgca gcgccctact
    19921 cttatcgtta aacaacaaca aactactcat ttaatttttt ctccttgccg atgatcctca
    19981 tcgtattcca accgaaactt tacctgattc tggcagtcaa atcggctatt acaaaacaaa
    20041 gataaggtaa ttcagtgaag aaaatcattt gtctggccat tacgctgcta atgacactcc
    20101 ccgcttacgc gaagttaact gttcatgaag aagcccgcat caacgccatg ctggagggat
    20161 tagcacagaa aaaggatttg atatttgtgc gcaacggtga tgaacatacc tgcgatgaag
    20221 cggtttccca cctgcgtctg aagctcggca atacccgtaa ccgcattgac actgccgagc
    20281 agtttattga taaggtagct tcgtcgtcat cgattaccgg gaagccgtat atcgtgaaga
    20341 tccccggtaa gagcgatgag aacgcacagc cttttttaca tgcgttaatt gcgcagacgg
    20401 ataaaacggt gcctgcggaa ggaaattaat ccgctttggt aaggcattta caggaggtaa
    20461 catgaaaaaa tgctttattt atcacgatga aaaatcgaat aaattttggt ggatagatta
    20521 cgaaggggat agtttagctg tcaactatgg caaggtaggt agtattggta aattccatac
    20581 aaaagaattc gataatgaag aacagtgtct gaaagaagcc agtaaattga ttgccgcaaa
    20641 aatgaaaaaa ggatatcaag aaactccaga ctttaacttc atggatctct actattttga
    20701 tgatgaagag attgggttac atcttaaaac gtcacaccca aacttccagt gccattttac
    20761 tgatccactt tatatgtgtt gctgggatga agaatctcct tttggtagcg atgaaggtgc
    20821 tgatgctcta aacgaacttg aaaatagcct ccgttctgga ctgtgctgat ttccctcaaa
    20881 tgttaattga aactatgtgg ggtatgaaat acatcgctat ggacagtatt cttgaagagg
    20941 atgtgcgtgc gcaattacta gccgatgaaa tgagctctat ccagagcaat atgattacct
    21001 acgcgactgc attcggtcag atcaaagtca tgggtaaaat ctcccataaa cttaaaaaga
    21061 tgggactcaa tgcactagcg cgtcatcagc ttaccgcaaa aattcttcaa tggggtgacg
    21121 gtcaggactc accaatactt caaaaaatga ttgatgacct tactgctttt ccacacgaaa
    21181 attaaaaact gcatttgtcg gcagcaacaa ctgccgacaa atcagattat tgacaaagtg
    21241 cactttgttt atgccggatg cggcgtaaac gcctgatccg gcataaaaat attgctaatt
    21301 caatatattg cagaaacatt gtaggcctga taagcggagc gcatcaggca gttttgcgtt
    21361 tgtcatcagt ctccaatgct attaatcctt aaatccctgc cccctgacta aaatgctctt
    21421 ccccaaacac tccggtggag aggtagcgat cgccacgatc gcagatgatc gctaccacca
    21481 ccgcaccagg gttagctttt gccacccgca gtgctccagc aaccgcgccg cctgagctga
    21541 cgccacagaa tattccttcc cgcaccgcca gttcgcgcat ggtgttttcc gcatcgcgct
    21601 gatgaatatc cagcacctca tccaccagcg cagcgttgaa aatccccggc agatattccg
    21661 caggccagcg gcgaatgccc ggaatactgc tgccttcttc cggttgcagg ccaacaatgg
    21721 tcaccggttt ggattgttcg cgcataaagc atgaaacgcc ggtgatagtg ccggtcgtcc
    21781 ccatgctgga gacaaaatga gtgatgcgcc c
//
LOCUS       DAZSNM010000052        21805 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZSNM010000052 DAZSNM010000000
VERSION     DAZSNM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21805
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.052"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            82..2682
                     /locus_tag="ACHW42_001648"
     CDS             82..2682
                     /locus_tag="ACHW42_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFR8943507.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYIAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3742
                     /locus_tag="ACHW42_001649"
                     /pseudo
     CDS             2673..3742
                     /locus_tag="ACHW42_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3754..4294
                     /locus_tag="ACHW42_001650"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="ACHW42_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="ACHW42_001651"
     CDS             4302..4817
                     /locus_tag="ACHW42_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8943508.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="ACHW42_001652"
     CDS             4783..5520
                     /locus_tag="ACHW42_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR8943509.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHW42_001653"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHW42_001653"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR8943510.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="ACHW42_001654"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="ACHW42_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR8943511.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHW42_001655"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHW42_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR8943512.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHW42_001656"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHW42_001656"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR8943513.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="ACHW42_001657"
     CDS             10827..12734
                     /locus_tag="ACHW42_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8943514.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHW42_001658"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHW42_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR8943515.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHW42_001659"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHW42_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR8943516.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHW42_001660"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHW42_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR8943517.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="ACHW42_001661"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="ACHW42_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR8943518.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHW42_001662"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHW42_001662"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR8943519.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="ACHW42_001663"
     CDS             complement(17402..19162)
                     /locus_tag="ACHW42_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR8943520.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="ACHW42_001664"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="ACHW42_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR8943521.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHW42_001665"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHW42_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR8943522.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHW42_001666"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHW42_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR8943523.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
BASE COUNT         5548 a         5140 c         5731 g         5386 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatattgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 tgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ccaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtg
//
LOCUS       DAZSNM010000053        21512 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZSNM010000053 DAZSNM010000000
VERSION     DAZSNM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21512
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.053"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACHW42_001667"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACHW42_001667"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR8943524.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="ACHW42_001668"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="ACHW42_001668"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR8943525.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACHW42_001669"
     CDS             complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACHW42_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR8943526.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="ACHW42_001670"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="ACHW42_001670"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR8943527.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="ACHW42_001671"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="ACHW42_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACHW42_001672"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACHW42_001672"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR8943528.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACHW42_001673"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACHW42_001673"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR8943529.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACHW42_001674"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACHW42_001674"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR8943530.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACHW42_001675"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACHW42_001675"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR8943531.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACHW42_001676"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACHW42_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR8943532.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="ACHW42_001677"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="ACHW42_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR8943533.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="ACHW42_001678"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="ACHW42_001678"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR8943534.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACHW42_001679"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACHW42_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR8943535.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACHW42_001680"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACHW42_001680"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR8943536.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACHW42_001681"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACHW42_001681"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR8943537.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACHW42_001682"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACHW42_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR8943538.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="ACHW42_001683"
     CDS             complement(18170..18565)
                     /locus_tag="ACHW42_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR8943539.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="ACHW42_001684"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="ACHW42_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8943540.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="ACHW42_001685"
     CDS             19481..20074
                     /locus_tag="ACHW42_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR8943541.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="ACHW42_001686"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="ACHW42_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR8943542.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21512
                     /locus_tag="ACHW42_001687"
     CDS             20794..>21512
                     /locus_tag="ACHW42_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR8943543.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5195 a         5324 c         5669 g         5324 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa gcattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat
    21361 cagaagttaa ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag
    21421 ttaaataatt cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct
    21481 acgattacgg atttattaga aaaagcattg aa
//
LOCUS       DAZSNM010000054        21253 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZSNM010000054 DAZSNM010000000
VERSION     DAZSNM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21253
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.054"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..791
                     /locus_tag="ACHW42_001688"
                     /pseudo
     CDS             1..791
                     /locus_tag="ACHW42_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(851..2149)
                     /gene="eno"
                     /locus_tag="ACHW42_001689"
     CDS             complement(851..2149)
                     /gene="eno"
                     /locus_tag="ACHW42_001689"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR8943544.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="ACHW42_001690"
     CDS             complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="ACHW42_001690"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR8943545.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="ACHW42_001691"
     CDS             complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="ACHW42_001691"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR8943546.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4989..7223)
                     /gene="relA"
                     /locus_tag="ACHW42_001692"
     CDS             complement(4989..7223)
                     /gene="relA"
                     /locus_tag="ACHW42_001692"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR8943547.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="ACHW42_001693"
     CDS             complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="ACHW42_001693"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR8943548.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8629..11385
                     /gene="barA"
                     /locus_tag="ACHW42_001694"
     CDS             8629..11385
                     /gene="barA"
                     /locus_tag="ACHW42_001694"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR8943549.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="ACHW42_001695"
     CDS             complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="ACHW42_001695"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR8943550.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12978..14318)
                     /locus_tag="ACHW42_001696"
     CDS             complement(12978..14318)
                     /locus_tag="ACHW42_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR8943551.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="ACHW42_001697"
     CDS             complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="ACHW42_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR8943552.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16106..16555)
                     /locus_tag="ACHW42_001698"
     CDS             complement(16106..16555)
                     /locus_tag="ACHW42_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR8943553.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16573..17355)
                     /gene="truC"
                     /locus_tag="ACHW42_001699"
     CDS             complement(16573..17355)
                     /gene="truC"
                     /locus_tag="ACHW42_001699"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR8943554.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17355..17684)
                     /locus_tag="ACHW42_001700"
     CDS             complement(17355..17684)
                     /locus_tag="ACHW42_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR8943555.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18306..18851)
                     /gene="syd"
                     /locus_tag="ACHW42_001701"
     CDS             complement(18306..18851)
                     /gene="syd"
                     /locus_tag="ACHW42_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR8943556.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18919..19767
                     /gene="queF"
                     /locus_tag="ACHW42_001702"
     CDS             18919..19767
                     /gene="queF"
                     /locus_tag="ACHW42_001702"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR8943557.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDKDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19879..21243
                     /gene="ppnN"
                     /locus_tag="ACHW42_001703"
     CDS             19879..21243
                     /gene="ppnN"
                     /locus_tag="ACHW42_001703"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR8943558.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5229 a         5670 c         5067 g         5287 t
ORIGIN      
        1 ttgcagccca accaactatt gtcccacaat tacagcaaca agttacgggt cttacgagta
       61 gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata
      121 cccaagtgca aattgctgta ttaattgttt ccacaaccaa agatgagaca attgaacaat
      181 atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac
      241 tgatcgttgt tgcctagtcg gatcgcactg tccgcatcca ggtaggttat gggctggaag
      301 aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta
      361 aacaacaaaa attagctaag ggattagagt tagctataat cgctttgaat aaccaactca
      421 cttcacaaca ccaatatccg gctaatcctt cagaaagtga atcaacgtct tccactgatc
      481 attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc
      541 atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca gcaatccatc
      601 ttttagattt attcctgttc tccgataaaa ctttttccat tgctgtattt tccttctttt
      661 ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatctg
      721 gtagaagtta tcattcagac aacagcggtt ccgctggagg ctcaagtggc ggcgcatccg
      781 gccgctggta actccaccat aaaaaatgcc agcccgaagg ctggcattat caaatcagat
      841 aaagtcagac ttatgcctgg cctttgatct ctttacgacc gttgtacggt gctttttcgc
      901 ccagagcttc ttcgatacga atcagctggt tgtatttagc aacacggtca gaacggctca
      961 tagaaccagt tttgatctgg cctgcagcag taccaacagc cagatcagcg atggtagcat
     1021 cttcagtttc accagaacgg tgagagataa ctgcagtgta gccagcatct ttcgccatct
     1081 tgattgcagc cagagtttcg gtcagagaac cgatctggtt gaatttgatc aggatggagt
     1141 tagcgatacc tttttcgata ccttctttca ggatcttggt gttggttacg aacaggtcgt
     1201 caccaaccag ctggattttg tcgcccagaa ctttggtctg gtatgcgaaa ccgtcccagt
     1261 cagattcgtc cagaccgtct tcgatagaaa cgatcgggta ctgtttggtc agttcttcca
     1321 ggaagtgagt gaattcttca gaggtgaacg ctttgttgcc ttcgccagcc agaacgtatt
     1381 taccatcttt gtagaattca gaagctgcgc agtccatcgc cagagtgatg tctttgccca
     1441 gttcataacc agcagcttta acagcttcag cgataacagc cagagcttca gcgttggaac
     1501 ccaggttcgg cgcatagcca ccttcgtcac caacagcagt gttcatgcct ttcgctttca
     1561 gaacttttgc caggtgatgg aaaacttcag aacccatgcg gatggcttct ttcacagttt
     1621 tcgcgccaac cggctgaatc atgaattcct ggatatcaac gttgttgtca gcgtgctcac
     1681 caccgttgat gatgttcatc atcggaaccg gcatggagta tttgcccgga gtgccgttca
     1741 gttcagcgat gtgctcgtac agcggcatac ctttagcagc tgcagcagct ttggcgttag
     1801 ccagagatac agccaggatt gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca
     1861 ggtcgatcat gatcttgtca atgccagcct gatctttagc atctttgcca atcagcgcct
     1921 gagcgatcgg gccgtttacc gcagcaacag ctttggttac gcctttaccc aggaaacggg
     1981 atttgtcgcc atcgcgcagt tccagagctt cacgggaacc agtagaagca cctgacggag
     2041 cagctgccat accgacgaaa ccaccctcca gatgtacttc ggcttcaaca gtcgggttac
     2101 cacgggagtc gatgatttca cgaccgatga tttttacgat tttggacatt aggttttcct
     2161 caagtcacta gttaaactga aactccagac aaacaacgcg tacccaaggt acgcgttgcc
     2221 gttctaactt ttttacttac ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa
     2281 agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt aaactccgga tggaactggc
     2341 aagccacgaa ccacgggtga ttcggaactt cgatgatctc gaccaactga tcatccccgg
     2401 aacggcccgc aacgcgcaga cctgcatctt caatctgttt caacagcatg ttgttgactt
     2461 cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt gtatagctgg cgaaccaggc
     2521 tatcgtcaac caactggcac tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct
     2581 tctcgctacg aacttcaacg ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa
     2641 ccgggtactt acagtctggc acaaattctg tagagttggc gttctccatg ttggcaacat
     2701 ggcgagcgta atcaattaac gccacctgca tacccaggca aatgcccaga taaggaatat
     2761 tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc tacgccacga tagccgaaac
     2821 cgccaggtac gaggattgcg tccagacctt taaggatttc aacgccgcgc gtttcaacat
     2881 cttgtgaatc gatcagtttg atgttgacgc tgacacgatt cttcagccca ccgtgtttca
     2941 gtgcttcgat cactgattta taagcatccg gcagttcaat gtacttgccg accataccga
     3001 tggtgacttc acttaccgga ttcgcttctt cgaagataac ctgttcccat tcggacagat
     3061 ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata atcgtccagc ccctgagatt
     3121 tcaacaggcc cggaatttta tagatggaat cgacgtcttt cagagaaata accgcttttt
     3181 ccggaacatt acagaacaat gcaatcttcg cacgttcgtt cgccggtaca gcgcgatctg
     3241 aacgacaaat caggatgtca ggctggatac cgatggagag cagctctttt acagagtgct
     3301 gagtcggttt ggttttgact tcaccagacg ctgccatgta cggcaccagc gtcaggtgca
     3361 taaacagagt gtgctcacgg ccaatttcaa cagccatctg gcgaatagct tcgaggaacg
     3421 gcaaggattc gatatcacct actgtaccgc cgatttctac cagtactacg tcatgacctt
     3481 cgccaccttc cagcacgcgc tctttgattg cgttagtgat gtgcggaata acctgcacgg
     3541 ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac gtcagagtag atacgacccg
     3601 tggtgaagtt gttgcggcgg ctcattttgg tacgaatgaa acgctcgtag tgccccaggt
     3661 ccaagtcggt ttcagcgccg tcttcagtaa cgaacacttc cccgtgttgg attgggctca
     3721 tagtacctgg atcgacgttg atgtaaggat ccagtttcat gatggtcaca ttgaggccac
     3781 gggcttcaag aatggctgcg agggaggctg cggcaatgcc tttacccaga gaggatacga
     3841 ccccgccggt cacaaaaata tagttcgttg tcatgctgaa cctgagaagt taggttgaaa
     3901 agacgatgga ataaccaaga cgggaaagca gtatacccga acatgacctg tgccacaaac
     3961 tttcattatc cctcctcttc gccagcgcac tattgaaatc aggagtgaga aaatagcccc
     4021 tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg
     4081 gcgcttgacc gcgtaattcc cttagagatc aatttcctgc cgttttacct gttgccagac
     4141 ttcttccatt gtttcgaggt caacacctgt catttccagt ccacgcgcgg caacaatacg
     4201 ctccacttcg cgaaaacgac gctcgaattt ttcgttcact ttttgcaatg cgatttctgc
     4261 tttcgtccct aaatggcgag ccagattaac cgtggcaaac agcaggtccc ccatttcctc
     4321 ctccagttta gcctggtcga caacagcctg ccgcgcctcg tacatcacct cgtcgatctc
     4381 ttcgtagact ttatcaacca ccgggccaag cgtcgtccag tcgaagccaa cgttggcgca
     4441 acgtttctgg attttttgcg cccgcattaa agccggtaaa ctacgaggaa tatcgtccag
     4501 cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg atttgctccc aacgggcaag
     4561 cacttcacta ctgttttcgg cagaactatc agcaaaaaca tgcggatggc gacgctctaa
     4621 tttatcgcta atagcagcgc aaatatcatt aaagtcaaag cgcccttctt cctgagccat
     4681 ttgcgcgtaa aacaccacct ggaatagcag atcgcccagt tcaccgcgaa gatcgtcaaa
     4741 atcttcacgg gcgatggcgt ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat
     4801 ggtggcaaat gtctgctctt tatcccacgg gcagccgttt tccggatcgc gcaggcgctg
     4861 cataatagtg agcaaacggt cgatttgatt cattgaattg tcctgaaaat tgcgggtctg
     4921 tcaggtgtaa taccttcgag taaaagatat cgctggatac ggctgacgcc atatccagct
     4981 atgtaagact aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     5041 ttacccagca cgcgccccag cacttgcagg ttgtaaatct caatggtcat gtcgatggtc
     5101 gccagttgct gtttggtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     5161 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5221 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5281 gcatgggagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgt
     5341 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5401 tggtgcatca ggttaccaac accttcaacc accacacgac cgttatcttt actgcggttt
     5461 tgcggtgtgt agcttttttg ctgaagctgt ttcagcgcgg cggcgtcctg ctcttcggca
     5521 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5581 ccaccaatcg ccgccagcaa ctcgtcgaca tcattgaagt tgtaacgcgg cagcagatgt
     5641 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5701 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc
     5761 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5821 tgtttctggg tgataatttc aatctggtcg cccatttgca gctggtaggt gaacggcaca
     5881 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5941 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     6001 acatacaccc ggtcgtcaaa cacctgacta cgtacttcgt cgagcatttc gccggaatca
     6061 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     6121 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6181 tccgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt tccacccggc
     6241 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacgtaatcg
     6301 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcataacaa
     6361 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcgaaggcg
     6421 aggttctttt tctgcatttt gcgccagatg ctgtagatgt gtttcggacg accatacact
     6481 tcagctttaa cgccttcagc tttcatctca gcgcgcagat ggccgacaaa ctcttcgata
     6541 tagtgttcgc ggtcgagacg gcgttcatgc agcagttttg cgatgcgttt gtattcggtc
     6601 gggtggagat aacggaagca gtaatcttcc agctcccatt tcagttgccc aatcccaaga
     6661 cggttcgcca acggcgcgta gatattggtg cactcttttg ccgccagtac acgttcatct
     6721 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6781 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6841 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6901 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6961 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     7021 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     7081 ccctgcgtct gttgcagaca atacgcccag gtttcggcta agcactcaca cgacttctgg
     7141 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7201 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7261 caaatcgtaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7321 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7381 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7441 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7501 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7561 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgcag
     7621 gaacaccttc tacaccgacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7681 ccataccgca gaacagatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7741 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7801 aagttaagcg caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta
     7861 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7921 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7981 ttgcctgtac cagttcaaca tgaccaagat ggcgcatagc ttgtaagctg cccagacatg
     8041 ccctgacttt gggcagcaat gcttcaagtt ggggcgctaa aatggggcat tgtttgacgt
     8101 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     8161 agtagttcag acttaaacgc gcgcggcggc gatagcccca gggaacatcg gcgatcactt
     8221 cagagacatc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8281 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8341 tttcgcgttc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8401 tatcttcagt aacagcaact tccgcgtttt cctgcggcaa taatccgggg ataaataacg
     8461 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8521 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8581 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8641 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8701 ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8761 ggtgctagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8821 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8881 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8941 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     9001 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     9061 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gcttgagctg
     9121 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttcctg cgtgatgatg
     9181 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9241 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9301 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9361 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9421 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttggatct ggcgaaaaag
     9481 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9541 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9601 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9661 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9721 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9781 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9841 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9901 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9961 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
    10021 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccgggctg
    10081 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
    10141 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10201 attatcgaag ggccatccat ccagtgcctc gcaggtaagc gcctggccta tgtcgaacca
    10261 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10321 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10381 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10441 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagttaatgc tgaaaaactc
    10501 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10561 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagt
    10621 aagctggcaa tgacagtcat ggcggttgat gacaaccccg ctaacctgaa acttatcggc
    10681 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcagtt
    10741 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gccggacatg
    10801 gatggcattc gggcctgcga actcatccac caactcccgc atcagcgaca aacgccggtt
    10861 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10921 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10981 aagcctggca gcggtatttc gtctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    11041 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    11101 ttagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    11161 gaggaacagc tggctggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11221 ggcagttgcg gctatagcgg cgtaccacgt atgaagaatc tctgccagct gatcgaacaa
    11281 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11341 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11401 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11461 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11521 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11581 accggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11641 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11701 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11761 acccagcccc ggtttttctg gcacctgtac cagcccgcct ttgatctcaa acggttcttt
    11821 ggtcaggcgc tgattacctt cctgccagat ccagtgcgta tcaatagcag taattttacc
    11881 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11941 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    12001 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    12061 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    12121 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc
    12181 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12241 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12301 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12361 atatttttca tacgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc
    12421 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12481 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12541 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12601 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12661 gcgcccacta gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12721 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12781 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12841 attacgcgtg aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc
    12901 cgccaccggg atgacctgca tttcagtaac aacaggcgtc gtaaattgag aactcatatt
    12961 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    13021 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgcaagg cagacgttta
    13081 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    13141 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13201 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13261 acatgggtaa atatcgccag agagatatcg aaatggttgt tggaatggca gccccaggtc
    13321 agcccccagt cgtcgcaaag ctgcgccacg cggactgcac cggaaagcgt ccagaagtgc
    13381 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13441 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgtcgaaa ctccgccatc
    13501 acttcacgtc cggagaagcc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13561 tcattcagcc ctttgcataa agaaatggct tcatcaagca gccatgcacc gttgggatca
    13621 acggtaatcc gcgcatccgg gaagcttttc ttcaatgcac gaacagtgtc gatttcttgc
    13681 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13741 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13801 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcacct
    13861 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13921 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13981 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccaaaggt atcaaaatcg
    14041 gctgcctgat tacctttatg tacctgctga accactttat tcaggcgcgc aacttcctgg
    14101 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    14161 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca cgatattgcg ggtgaagtat
    14221 gcgttatgcg caccaccaat attgagcaac atgctgtcat gtccggcgac cggaatgact
    14281 ttcatatcag taataacagg actggattgt gtcgccatct tattgccccg cgacaggttt
    14341 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattagggc
    14401 atgaacacca acataaatca gcgccccatt aaacgagcca gtcgtgccaa cgatataacc
    14461 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14521 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14581 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14641 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14701 aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa ataattccac ccagcacacc
    14761 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14821 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14881 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gctgtttgat
    14941 ctgcccccac ttcacgctga acggcacttt aactttggtg ctttgctgat ccatattgat
    15001 cagcgcgcca cccgcggcga tatactccag ctctttctga tttacccccg gatgttgatt
    15061 tggctcgtgg atgactttca accagataaa gctgatgaca atgcccagac cgcccataaa
    15121 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15181 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcac
    15241 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15301 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15361 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15421 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15481 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15541 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15601 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15661 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15721 acatcgccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15781 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15841 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15901 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gtaaaaacga
    15961 agaaataaag aagagtatga aatggatcgc ttgactctag gcaaacgcca gtaaaaatcc
    16021 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    16081 acgacattta ccgctcgctg acatatcagg acaacagcgt gccccactgt tcgacccacg
    16141 gatttgattc cgtttccggt tccgggttct cactggcgtc aatcaacagc atttcgccaa
    16201 cccgctgagc gctctgttcc tgcaacaagg catcgaactg tttgccgcca ttgcaaaaat
    16261 ttacataact gctgtcgccg agcgcaatca cgccataacg cagattcggc tggaagccca
    16321 gactatcttt gattccctga aagagcggca caatgctatc aggaaggtcg ccctgcccgg
    16381 tcgtggacgt aaccaccaga acatacttat cctgataggg cagccagtcg cttaattcag
    16441 gatcttcaaa taccgttgct ttgtggccct gtgcggtcag aattgcttct gcttcttccg
    16501 ccactaacag cgaatttccg tacatggtgc cgacaaaaat accaatttcc gccataccgt
    16561 tttctccctg gattaggaac ttatctctcc atcctgaccc gatggcgcac tgaactcaac
    16621 cctttcattt tcaggaagca gaccgcgcca gccaaattgt gataacgcct gcatccaggt
    16681 gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta aaaggatgcg tcaatgacaa
    16741 ctgactggca tgtagcatta accgctggag gccaaaatgc tcagcaccgc tgcgattctg
    16801 gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga cgcaaatggg caagatgtcg
    16861 tcgaagctga tgtttgcgtc cggttttcgg ctccagttcc accaggccgt agcgcgtggt
    16921 cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac
    16981 tgctggctgc gggcctttat cttcgcgggc aaatttatca gcgattttgt ccagttcttc
    17041 caccagtgga taatccaaca ccgcttcttc catcaaccag ccgcgcacaa tcgcatggta
    17101 acgtttctgg atttggtgct gttcaaactg ttgtgccagc agccgtccgg cctcgctgga
    17161 taatcccatc aacaacacac cagaagtggg tcggtccaga cgatgagcag taaaaacatg
    17221 ctgtcctatc tggtcacgca cggtttgcat gaccactact ttctcgtcgc gatccagcca
    17281 gctacggtga accagccagc cggagggttt atttaccgca accagccatt catcctgata
    17341 gagtatttcc agcattagct cgcatcatcc gcaaaaagag catccagttt ttccaactca
    17401 gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg ccatttcata atagggtgca
    17461 acagcaaaag cgcccggtaa cggctggttg ttatttaaca aatcgtgcat tcgcgggatc
    17521 agcccccact gcaaccactc cagtggttcc atggtgtcca taaagaacgg ttgggtacta
    17581 ttaaattgat gcggctgggg ttcatcgttt cgccagtgct gatgttcacg cagtaacgct
    17641 tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg tcataaagca acctcaataa
    17701 gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga gcactgtatt cacagtgctc
    17761 ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc ctgctcatcc ttgacaactt
    17821 ttcctctggc cttgcggcca atcgttcatc ctgaactatt gcttcctgct cacaccaccc
    17881 cgatgtgata cttcatcctg aagtgtccct ggccttcctg acccaccgaa tcatcctgac
    17941 cggttctcat tctccatcct ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct
    18001 tcatccagaa gcctttccct gaaacaccat cctggtgtgt cctgcagaag tgtcatcatc
    18061 ctgatgttca cttcgttgtc tgactccctg tcgacgaaga tagaatcgtc tttttcgcga
    18121 agtcttacaa ggcgcttaca gacaatgcct taaggaaaat ttcatatgaa agttaagaat
    18181 taacaatcaa cattttgatt ttaaatgaaa ataacgattc ttatcttttt aagtattcgc
    18241 ttatttcctg gcgatctcct acagagtctg taagagatct ctcacaacca gatggggtat
    18301 ttggattaca gaagaggctt aagttgatta aggaattccg caagatttga ggctaaattc
    18361 gtccgtttac gtgttcccag cgtctctttg cacacttcac cggagagatt acacaccgag
    18421 atgacctcta actcttcttc cagcgtcgcg ataaatagcg tgggcggcag ttttagcctt
    18481 ttctgagtca ccagatgacc aatcagattt tcctgcaccc gacggaagtc gtcttcactc
    18541 caggtttgca gcaatgtcag tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac
    18601 tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc
    18661 gcgtttacat tttgttcccc ggtaaacggc tggggttgcc agtatacggc atcttcggta
    18721 gttgaaataa tgcacggtga aggaacaccg tacaattcct cacttaacgg ccagctttta
    18781 tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc
    18841 aaatcgtcca ctaatttctc tcttcactta aaccagatac acttgtcgtt tagtttatct
    18901 ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc aggcacttgc gggcctgact
    18961 cttggaaaat caaccgatta ccgggatacc tatgacgcca gcctactgca aggcgttcca
    19021 cgcagcctga atcgcgaccc gctgggtctg aaagcggata acctgccttt tcacggtacg
    19081 gatatctgga cgctgtatga actttcctgg ctgaatgcga aaggtttgcc gcaggtcgct
    19141 gtcggtcatg ttgaacttga ttacaccagc gtaaatctga ttgagtcgaa gagttttaag
    19201 ctctatctca acagttttaa ccagacgcgt tttaataact gggatgaggt gcgccagacg
    19261 ctggagcgcg acttaagcac ttgcgctcag ggtaagatta gcgtggcgtt atatcgtctt
    19321 gatgaactgg aaggccagcc gataggtcat ttcaatggca cttgcattga tgacaaggat
    19381 atcactatcg ataactatga attcactact gactatctgg agaatgccac cagcggtgaa
    19441 aaagtagtgg aagagacgct cgtcagccac ctgctgaaat caaactgcct gatcacccat
    19501 caaccagatt ggggttcgat ccaaattcag tatcgtggac gccaaattga cagagaaaaa
    19561 ctgctgcgtt acctggtctc attccgtcat cacaacgagt tccacgaaca gtgcgtggaa
    19621 cgcatcttta atgacctgtt acgcttctgc cagccagaga aattgagcgt ttacgcacgt
    19681 tatacccgtc gtggcggtct ggacattaac ccgtggcgca gtaatagcga ttttgtccca
    19741 tcgaccacaa gactggttcg gcaataaatt ttttctcaat tttgcgtgct ggattcacgc
    19801 agaaggttgt gaaaggtcat caggcagggc tattgtaatc aaagggaatg acgatattcg
    19861 tcccataagg agtttttctt gattacacat attagcccgc ttggctccat ggatatgttg
    19921 tcgcagctgg aagtggatat gcttaaacgc accgccagca gcgacctcta tcaactgttt
    19981 cgcaactgtt cacttgccgt actgaactcc ggtagtttga ccgataacag caaagaattg
    20041 ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc gtgaacgcgg cgtaaagctg
    20101 gaactgatta atcccccgga agaggctttt gtcgatgggc gaattattcg cgctttgcag
    20161 gccaacttgt tcgcggttct gcgagacatt ctcttcgttt acgggcaaat ccatcatacc
    20221 gttcgttttc ccaacctgaa tctcgacaac tccgtccaca tcactaacct ggtcttttcc
    20281 atcttgcgta acgctcgcgc gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg
    20341 ggcggtcact caattaacga aaacgagtat ttgtatgccc gtcgcgtcgg gaaccagctg
    20401 ggcctgcgtg agctgaatat ctgcaccggc tgtggtccag gagcgatgga agcgccgatg
    20461 aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag acagtcgttt tattggtatg
    20521 acagagccgt cgattatcgc cgctgaaccg cctaacccac tggtcaacga attgatcatc
    20581 atgccggata tcgaaaaacg tctggaagcg tttgtccgta tcgctcacgg catcattatc
    20641 ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt tgctgggaat tttaatgaac
    20701 ccggccaaca aagatcaggt tttaccattg atcctcaccg gcccgaaaga gagcgccgac
    20761 tacttccgcg tactggacga gtttgtcgta catacgctgg gcgaaaacgc gcgccgccat
    20821 taccgcatca tcattgatga cgccgctgaa gtcgctcgtc agatgaaaaa atcgatgccg
    20881 ctggtgaaag aaaatcgccg tgatacaggc gatgcctaca gctttaactg gtcaatgcgc
    20941 attgcgccag atttgcaaat gccgtttgag ccgtctcacg agaatatggc taatctgaag
    21001 ctttacccgg atcaacctgt tgaagtgctg gctgccgacc tgcgccgtgc gttctccggt
    21061 attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca ttgaagagtt tggtccttac
    21121 aaaatcaacg gcgataaaga gattatgcgt cgtatggacg acctgctaca gggttttgtt
    21181 gcccagcatc gtatgaagtt gccaggctca gcctacatcc cttgctacga aatctgcacg
    21241 taatctcccc tcc
//
LOCUS       DAZSNM010000055        20989 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZSNM010000055 DAZSNM010000000
VERSION     DAZSNM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20989
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.055"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            105..2639
                     /gene="mrcB"
                     /locus_tag="ACHW42_001704"
     CDS             105..2639
                     /gene="mrcB"
                     /locus_tag="ACHW42_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR8943559.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2859..5102
                     /gene="fhuA"
                     /locus_tag="ACHW42_001705"
     CDS             2859..5102
                     /gene="fhuA"
                     /locus_tag="ACHW42_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR8943560.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            5153..5950
                     /gene="fhuC"
                     /locus_tag="ACHW42_001706"
     CDS             5153..5950
                     /gene="fhuC"
                     /locus_tag="ACHW42_001706"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR8943561.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5950..6840
                     /gene="fhuD"
                     /locus_tag="ACHW42_001707"
     CDS             5950..6840
                     /gene="fhuD"
                     /locus_tag="ACHW42_001707"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR8943562.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6837..8819
                     /gene="fhuB"
                     /locus_tag="ACHW42_001708"
     CDS             6837..8819
                     /gene="fhuB"
                     /locus_tag="ACHW42_001708"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR8943563.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8854..10134)
                     /gene="hemL"
                     /locus_tag="ACHW42_001709"
     CDS             complement(8854..10134)
                     /gene="hemL"
                     /locus_tag="ACHW42_001709"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR8943564.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10300..10347
                     /gene="yadX"
                     /locus_tag="ACHW42_001710"
     CDS             10300..10347
                     /gene="yadX"
                     /locus_tag="ACHW42_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR8943565.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            10359..11780
                     /gene="clcA"
                     /locus_tag="ACHW42_001711"
     CDS             10359..11780
                     /gene="clcA"
                     /locus_tag="ACHW42_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR8943566.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11804..11869
                     /gene="yadW"
                     /locus_tag="ACHW42_001712"
     CDS             11804..11869
                     /gene="yadW"
                     /locus_tag="ACHW42_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR8943567.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            11862..12206
                     /gene="erpA"
                     /locus_tag="ACHW42_001713"
     CDS             11862..12206
                     /gene="erpA"
                     /locus_tag="ACHW42_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR8943568.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12253..12876)
                     /locus_tag="ACHW42_001714"
     CDS             complement(12253..12876)
                     /locus_tag="ACHW42_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR8943569.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12914..13714)
                     /gene="btuF"
                     /locus_tag="ACHW42_001715"
     CDS             complement(12914..13714)
                     /gene="btuF"
                     /locus_tag="ACHW42_001715"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR8943570.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13707..14405)
                     /gene="mtnN"
                     /locus_tag="ACHW42_001716"
     CDS             complement(13707..14405)
                     /gene="mtnN"
                     /locus_tag="ACHW42_001716"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR8943571.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14489..16006
                     /gene="dgt"
                     /locus_tag="ACHW42_001717"
     CDS             14489..16006
                     /gene="dgt"
                     /locus_tag="ACHW42_001717"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR8943572.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            16136..17560
                     /gene="degP"
                     /locus_tag="ACHW42_001718"
     CDS             16136..17560
                     /gene="degP"
                     /locus_tag="ACHW42_001718"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR8943573.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            17715..18871
                     /gene="cdaR"
                     /locus_tag="ACHW42_001719"
                     /pseudo
     CDS             17715..18871
                     /gene="cdaR"
                     /locus_tag="ACHW42_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(18960..19346)
                     /locus_tag="ACHW42_001720"
     CDS             complement(18960..19346)
                     /locus_tag="ACHW42_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR8943574.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(19660..20484)
                     /gene="dapD"
                     /locus_tag="ACHW42_001721"
     CDS             complement(19660..20484)
                     /gene="dapD"
                     /locus_tag="ACHW42_001721"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR8943575.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(20515..>20989)
                     /locus_tag="ACHW42_001722"
     CDS             complement(20515..>20989)
                     /locus_tag="ACHW42_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFR8943576.1"
                     /translation="SVHDAQIFTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQV
                     LTQSSWQPPQPRRQPAKLRHFTVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIF
                     ADLGISLHGARITTIGERVEDLFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
BASE COUNT         4798 a         5257 c         5826 g         5108 t
ORIGIN      
        1 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
       61 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
      121 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
      181 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
      241 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
      301 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
      361 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
      421 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
      481 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
      541 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
      601 aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
      661 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
      721 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
      781 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
      841 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
      901 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
      961 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     1021 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     1081 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     1141 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     1201 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     1261 ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgtggtg
     1321 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     1381 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     1441 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     1501 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     1561 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     1621 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     1681 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     1741 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     1801 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     1861 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     1921 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     1981 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     2041 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     2101 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     2161 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     2221 tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     2281 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     2341 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     2401 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     2461 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
     2521 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
     2581 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
     2641 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
     2701 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
     2761 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
     2821 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
     2881 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
     2941 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
     3001 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
     3061 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
     3121 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
     3181 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
     3241 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
     3301 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
     3361 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
     3421 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
     3481 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
     3541 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
     3601 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
     3661 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
     3721 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
     3781 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
     3841 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
     3901 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
     3961 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
     4021 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
     4081 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
     4141 actctgtgcc actgttcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
     4201 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
     4261 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
     4321 gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt gatgacaaac
     4381 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
     4441 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
     4501 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
     4561 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
     4621 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
     4681 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
     4741 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
     4801 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
     4861 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
     4921 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
     4981 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
     5041 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
     5101 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
     5161 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
     5221 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
     5281 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
     5341 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
     5401 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
     5461 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc
     5521 agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
     5581 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
     5641 gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca
     5701 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc
     5761 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag
     5821 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
     5881 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
     5941 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
     6001 ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga ccccaatcgt
     6061 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
     6121 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
     6181 gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg
     6241 tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg
     6301 ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg
     6361 ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa
     6421 tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
     6481 ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc
     6541 caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
     6601 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
     6661 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
     6721 atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca
     6781 acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga
     6841 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg
     6901 cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct
     6961 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc
     7021 tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
     7081 tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg
     7141 ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc
     7201 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt
     7261 cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc
     7321 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
     7381 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
     7441 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
     7501 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
     7561 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
     7621 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
     7681 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
     7741 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
     7801 tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga
     7861 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg
     7921 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
     7981 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
     8041 tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
     8101 cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg
     8161 taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc
     8221 tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg
     8281 cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
     8341 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
     8401 ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc
     8461 gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc
     8521 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
     8581 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
     8641 gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
     8701 tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
     8761 tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat
     8821 agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc
     8881 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcctgcttc
     8941 aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa
     9001 acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
     9061 gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc
     9121 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc
     9181 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
     9241 aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg
     9301 accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc
     9361 tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac
     9421 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
     9481 ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
     9541 aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca
     9601 ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt
     9661 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
     9721 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt
     9781 cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
     9841 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
     9901 ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca
     9961 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    10021 tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    10081 accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    10141 ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat
    10201 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    10261 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    10321 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    10381 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    10441 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    10501 tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    10561 tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg
    10621 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg
    10681 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat
    10741 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg
    10801 aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc
    10861 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    10921 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    10981 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    11041 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    11101 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    11161 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    11221 ttaccaaatg ggtactgatg gggggggcga ttggcggtct gtgtggattg ctggggtttg
    11281 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt
    11341 tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct
    11401 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    11461 gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga
    11521 cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg
    11581 ggatcattct ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    11641 ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta tactcggcga
    11701 ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat
    11761 cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg
    11821 gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac
    11881 tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag
    11941 ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt
    12001 atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg
    12061 ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactaca
    12121 ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg
    12181 gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca
    12241 catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca
    12301 acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc
    12361 cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc
    12421 agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta catcacgaat
    12481 gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc
    12541 cgcattaaag gctttattca cgctaatgcc gacaaacacc gccagaccaa cggcgtccag
    12601 caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat
    12661 gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg
    12721 atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc ccagtaccag
    12781 aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga tggcaaatac
    12841 cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg
    12901 aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt gctgcgagga
    12961 taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga
    13021 gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa
    13081 tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc
    13141 tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga atcgactctt
    13201 ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat
    13261 cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg gcttgttcgg
    13321 ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa
    13381 tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc
    13441 gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt
    13501 ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt
    13561 cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg
    13621 cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg agaaaagaca
    13681 gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac cagtgactca
    13741 accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac
    13801 tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg
    13861 cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg
    13921 cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca
    13981 cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg
    14041 tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg
    14101 acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt
    14161 ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac
    14221 agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc
    14281 tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg
    14341 ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt
    14401 ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg
    14461 gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa
    14521 ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat
    14581 cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa
    14641 gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat
    14701 ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc tgaaagagct
    14761 taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga
    14821 gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc
    14881 gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac
    14941 tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga
    15001 gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg
    15061 tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg gtattttaaa
    15121 atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa
    15181 aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta aagaacttaa
    15241 tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg acgacatctc
    15301 ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct
    15361 ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt
    15421 ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca
    15481 gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg
    15541 atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga
    15601 tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt
    15661 tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt
    15721 agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa
    15781 agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct cgacgcgcca
    15841 tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct
    15901 atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct
    15961 ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag tcttttgtaa
    16021 agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc gggctataaa
    16081 acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa
    16141 aaaaaacaca ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct
    16201 ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc
    16261 accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac
    16321 cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt
    16381 ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa
    16441 tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga
    16501 taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca
    16561 gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat
    16621 cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg cggattctga
    16681 tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac
    16741 ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga
    16801 aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa
    16861 cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat
    16921 cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga
    16981 atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact
    17041 ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa
    17101 ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa
    17161 gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa
    17221 actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca
    17281 gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat
    17341 gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg gcactccggc
    17401 tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc aggcagtgaa
    17461 aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat
    17521 tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg
    17581 aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca
    17641 tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc
    17701 ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg
    17761 cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta
    17821 tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt
    17881 cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac ggtgtgcggc
    17941 aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag
    18001 gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc
    18061 tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaagaactgg
    18121 tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg cgcaacggct
    18181 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    18241 tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    18301 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    18361 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    18421 ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg
    18481 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    18541 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    18601 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    18661 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca
    18721 accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa
    18781 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    18841 tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag gcggcgctgg
    18901 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat
    18961 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    19021 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    19081 cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    19141 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    19201 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    19261 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    19321 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    19381 tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    19441 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    19501 atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    19561 tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgagccaga
    19621 aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg tacgcagcag
    19681 ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca
    19741 gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc
    19801 cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc
    19861 aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt caaccacttc
    19921 agagcgtgcg ccaatgaagc agttatcttc aataatggtc gggttagcct gcagcggctc
    19981 cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca
    20041 agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt
    20101 aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc
    20161 tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt
    20221 catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat
    20281 acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat
    20341 tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc
    20401 gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc
    20461 aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc
    20521 tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt
    20581 attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat
    20641 ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg
    20701 cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg
    20761 ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg
    20821 acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac caaaccgaat
    20881 aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc
    20941 catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactt
//
LOCUS       DAZSNM010000056        20834 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZSNM010000056 DAZSNM010000000
VERSION     DAZSNM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20834
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.056"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..576
                     /locus_tag="ACHW42_001723"
                     /pseudo
     CDS             1..576
                     /locus_tag="ACHW42_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            679..1002
                     /locus_tag="ACHW42_001724"
     CDS             679..1002
                     /locus_tag="ACHW42_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HFR8943577.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1069..2526)
                     /gene="nuoN"
                     /locus_tag="ACHW42_001725"
     CDS             complement(1069..2526)
                     /gene="nuoN"
                     /locus_tag="ACHW42_001725"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR8943578.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2533..4062)
                     /gene="nuoM"
                     /locus_tag="ACHW42_001726"
     CDS             complement(2533..4062)
                     /gene="nuoM"
                     /locus_tag="ACHW42_001726"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR8943579.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4226..6067)
                     /gene="nuoL"
                     /locus_tag="ACHW42_001727"
     CDS             complement(4226..6067)
                     /gene="nuoL"
                     /locus_tag="ACHW42_001727"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR8943580.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6064..6366)
                     /gene="nuoK"
                     /locus_tag="ACHW42_001728"
     CDS             complement(6064..6366)
                     /gene="nuoK"
                     /locus_tag="ACHW42_001728"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR8943581.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6363..6917)
                     /gene="nuoJ"
                     /locus_tag="ACHW42_001729"
     CDS             complement(6363..6917)
                     /gene="nuoJ"
                     /locus_tag="ACHW42_001729"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR8943582.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6929..7471)
                     /gene="nuoI"
                     /locus_tag="ACHW42_001730"
     CDS             complement(6929..7471)
                     /gene="nuoI"
                     /locus_tag="ACHW42_001730"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR8943583.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7486..8463)
                     /gene="nuoH"
                     /locus_tag="ACHW42_001731"
     CDS             complement(7486..8463)
                     /gene="nuoH"
                     /locus_tag="ACHW42_001731"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR8943584.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8460..11186)
                     /gene="nuoG"
                     /locus_tag="ACHW42_001732"
     CDS             complement(8460..11186)
                     /gene="nuoG"
                     /locus_tag="ACHW42_001732"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR8943585.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11239..12576)
                     /gene="nuoF"
                     /locus_tag="ACHW42_001733"
     CDS             complement(11239..12576)
                     /gene="nuoF"
                     /locus_tag="ACHW42_001733"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR8943586.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12573..13073)
                     /gene="nuoE"
                     /locus_tag="ACHW42_001734"
     CDS             complement(12573..13073)
                     /gene="nuoE"
                     /locus_tag="ACHW42_001734"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR8943587.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13076..14878)
                     /gene="nuoC"
                     /locus_tag="ACHW42_001735"
     CDS             complement(13076..14878)
                     /gene="nuoC"
                     /locus_tag="ACHW42_001735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR8943588.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14972..15634)
                     /gene="nuoB"
                     /locus_tag="ACHW42_001736"
     CDS             complement(14972..15634)
                     /gene="nuoB"
                     /locus_tag="ACHW42_001736"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR8943589.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15650..16093)
                     /gene="nuoA"
                     /locus_tag="ACHW42_001737"
     CDS             complement(15650..16093)
                     /gene="nuoA"
                     /locus_tag="ACHW42_001737"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR8943590.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16724..17662)
                     /gene="lrhA"
                     /locus_tag="ACHW42_001738"
     CDS             complement(16724..17662)
                     /gene="lrhA"
                     /locus_tag="ACHW42_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR8943591.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18581..19798
                     /gene="alaA"
                     /locus_tag="ACHW42_001739"
     CDS             18581..19798
                     /gene="alaA"
                     /locus_tag="ACHW42_001739"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR8943592.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19882..20481
                     /gene="yfbR"
                     /locus_tag="ACHW42_001740"
     CDS             19882..20481
                     /gene="yfbR"
                     /locus_tag="ACHW42_001740"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR8943593.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20540..>20834)
                     /locus_tag="ACHW42_001741"
     CDS             complement(20540..>20834)
                     /locus_tag="ACHW42_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR8943594.1"
                     /translation="VLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMA
                     ASAAFMTPVSSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
BASE COUNT         5273 a         5721 c         5300 g         4540 t
ORIGIN      
        1 cctgggtcac tgcattatcg ccaacgcccc gacacgtaag tcctgagcaa ttagaaaaaa
       61 cagttcgtta tcttacacaa actgtgcatc cacgtaatgc cggcaatatt gataatctga
      121 ataggtccgc agagtatata aaagagttct ttgtcagtag cggtgccaga gttaccgcgc
      181 agggcgtccc cgattggcta ttacgactct gcgcctggaa gccagaatta cccttacccg
      241 gcaatgtcct ggctttatcc cgatcgggga gactttattg ctgtggtcgg cagaatgcag
      301 gatatcaacg ccgttcgtca ggtaaaagcg gcattgttgt catcccggga tttatctgtt
      361 tattctatga atgccccagg gtttattccc ggtattgatt tttctgatca cctcaattat
      421 tggcaacacg atattcccgc cgtaatgatt actgacaccg ctttttatcg taataaacaa
      481 taccacttgc ccggtgatac cgcagacaga ttgaattatc agaaaatggc tcaggtggta
      541 gatggtgtta taaccttgtt atacaacagt aaataataaa aatttctcga ggcgggtcca
      601 gaaacagcga catgtacatt ttattttccc tcgtctgttt taagatgcga aacataaaat
      661 tcaatggagg aaaaataaat gggaatgatt ggctattttg cagaaattga ctcagaaaag
      721 attaaccaac tgcttgagtc tactgaaaaa cctttgatgg ataatataca tgacacgctt
      781 tcaggattac gccgactgga atcactggat cgaagtgagc tgcgtaaaca gttttccatt
      841 aagcgactca atgaaatgga gatttatccg ggagtgacct tttcagaaga gttagagggg
      901 caactctttg cctcgatcat gcttgatatg gaaaagctga ttagcgcgta tcgacgtatg
      961 ctgcgacaag ggaatcatgc gctgacggtt attgttgggt gatggtgtca gagcgccaaa
     1021 aaaaaccgcc gaacatgtcg gcggctttct gacttacaaa ctaacagttt acatcagcgg
     1081 cattgctaac cgcacaatac taatcagcgg ttgtggccat acacccagca ccagtaccaa
     1141 cagtgcagaa atcagcacca cgataccgcc cgcgctgtac tgccagtttg acggtgcatc
     1201 gcgacccggt tgttccggtg cgtgcaggta caggctcacc gccacgcgca ggtagtagta
     1261 gaggccgatt gccgaaccga caaccacggc acccaccagc caccacaagt gtgcctggac
     1321 accgaccgcc agcacgtaga acttaccgat aaagcccagc gtcatcggga taccggccag
     1381 agacagcatc atcaccgtca tcactgccgc gaggatcgga cgatgccaga acagaccgcg
     1441 gtaagagaac agggaatcag catccgggcc acgatacggg ctggacatca ggctgaccac
     1501 gccgaacgcg ccgaggctgc tgaacagata accggccagg taaaccccta ccgcttccat
     1561 cgacatctcg ccggtttgca gcgcaatcag cgccaccagc agatagccga ggtgagagat
     1621 agatgagtaa ccgagcagac gtttgatgtt ggtctggctc aacgccatca ggttaccgaa
     1681 gatgatggag gcaaaggcga taatcgccag tactacgcga atcgcttcgc tgtcacccac
     1741 cggtgcgtag aggaacagac gcatcaccac accgaagata gcgattttgc tcgccgtcgc
     1801 caggaaagtg gaaaccggcg caggcgcgcc ctggtatacg tctggcgtcc acaggtggaa
     1861 cggcaccaga gagagtttga agccgaggcc aacaatcatc atgccgaaac ctgccagcaa
     1921 cagcggctcg ttgagcatac cgtcgccgag gtttttaccc agcgcgacaa acgacaggtc
     1981 gccagactgc gcatacacca gcgccatacc gaacagcagg aaagaagacg ctgcggcaga
     2041 aaggatggtg tatttgatac tggcttccag cgaacgtttc tggcggaaag cgtaaccgac
     2101 caggccaaac agcggcaaag agatcagttc aataccgagg aacagagacg ccagatggtt
     2161 tgcattcgcc agcaggatcc cgcccagcgc ggcaattaac accaacaggt agaactcatc
     2221 cttgttgtcg ttatagcctt caagccacgg gtaggcgaaa gtacaggtgg cgaggctcgc
     2281 caacaatacc agcccggtgt aaagcatggc gaaaccatca acgcgcatca gcggcgtaac
     2341 gtccatagcg cccgcctggc caacaaacca gagcgaaacc agcgccgcgt taagcccaat
     2401 aaccgagagc gtagcgttga ggaaatgatt gcgtcgccac gcaatggaga gcatcacaac
     2461 caccaccgtc aagccgacga tcagcaacgg tagcagtgcg atcaggtttt gtggagttat
     2521 tgtcatggcg atttacggcc ttgtagtagt aacggaatta acaaaccact gctggatatt
     2581 gccaatcgcg gagtgcgagg tatccagaat cggctgcgga tagaagccca gcagtaccag
     2641 cagcaccacc agcaacagga tcataaacag ctcacgcagc gacatccctg gcagttcctg
     2701 gctggcaatc tggcttttcg ctttaccgaa gtaagcgcga tgcaacatcg ccagcgaata
     2761 aacagatgca aagaccagcc caaaggtaga gataacggta atcaccggga caacctggaa
     2821 gctgccgaac agaatcataa attcgccgac gaagttaccg gtgccaggca tcccaagcgt
     2881 tgccaccgca aagaacagcg acagtgctgg cagccatttc atcttgctcc acagaccgcc
     2941 catcatgcgc atgtcgcggg tatggatacg ttcataaagc tgaccgcaga gaataaacag
     3001 acccgccgcc gacaagccgt gcgcaatcat ctggattacc gcgccctggt aggccaactg
     3061 gctgccggtg tagattgcaa tcagcacgaa gcccatgtgg gaaaccgagg tgtaggcgat
     3121 cagacgtttg atatcggtct gagcgaaggt catccacgca ccgtagaaga tgccgataac
     3181 acccagccac atagcgattg gcgcgaactc tgccgacgcg ttcgggaaca gcggcaggga
     3241 gaaacgcaac aaaccgtaag cggcggtttt cagcaagatc cccgcgaggt ccacggaacc
     3301 ggcggtcgga gcctgggagt gcgcatccgg cagccagcca tgcagcggaa ccaccggcat
     3361 tttgactgcg aaggcgatga agaagcccag catcaacaga tattccacac cactggacat
     3421 tggcgtattc agcagctctt catagttgaa ggtccagacg ccggtcgcat tgtagtgaac
     3481 aaaaaccagc gccaggatgg cgatcaacat caccagacca ctcgcctggg tgtaaatgaa
     3541 gaacttggtt gccgccgtga tacgcgtttt accgtcagag gctttatgcc cccacagtgc
     3601 gatcaggaag tacatcggca ccagcatcat ttcccagaag aagaagaaca ggaacatgtc
     3661 gatggcaagg aacacgccga taacgccgcc caggatccac atcaggttga ggtggaagaa
     3721 gccctgatat ttttcgatct ctttccacga acatagtacc gccagcacac cgagcagacc
     3781 ggtcagcacg accatcagca gcgacagacc gtcaatggcg agatgaatag agataccaaa
     3841 acgcgggatc cacggcatgt cgaattcaga ctgccactgc ggaattccgg cggattgcgt
     3901 cagtgaataa ccgccctgca accacagttg cagcgacagc gccagcgtca atcccatggt
     3961 gatcagcgcg atccagcgtg gcaccttgac gccaaagcgt tcggtctgcc agcacaggaa
     4021 gccgccaata aagggaatta atattagcca gggtagtaac atggcgatct ttattccttg
     4081 taaaagtccc gtcaggaccg gattttcaac gaattctcac gacagaaaat tctcttcgga
     4141 ctgggggtag tgtcggatgc gccgcgaagg cagcttatcc gacctacgtt cttatatgcc
     4201 caggggcaat cccacaatcc ttaactcaac gcagtaccat caacagtgcc agcaccacga
     4261 ccgcaccgat gctcatggat gccacatacc agcgcagata accgttctcg cttaacagca
     4321 gacctttacc tgcaaagcgg gaaagaaccg ccgggatgtt catcattgag ttcagcggat
     4381 cgcgtttcag caaccaggca atacccagga acggcttgac gaacactttg tcgtacagcc
     4441 agtcaaagcc ccaagcgttg taccaccagg tactcagcag gcggcccggc gcactgttgg
     4501 cgatggaggt caccagagaa cgtttaccca gccacagcca ggctgccagc agaatgccga
     4561 ccaccgcgac cacgccagag gtaatttcca gggtcaacat gctgccgtgc gccagttccg
     4621 tcgtttgcgg aagcacgccc tgcagcggcg gtacaatcag tgcgccaacg aaggtggaaa
     4681 ggatcagcag cacaatcagc ggcaggctgt gagttacccc tttcacggcg tgagcgtgaa
     4741 tttgttcttt tccgtggaag acgatgaaaa tcatacggaa ggtgtagagc gaggtcataa
     4801 acgcaccgac cagacctgcc accatcagat tgatatgacc attcgccatc gcacccgcga
     4861 ggatctcatc cttactgaag aagcccgcag tgaccagcgg cagtgccgac agtgctgcgc
     4921 cgcccaccag gaagcagaga taaaccagcg gaatagattt acgcagaccg cccatcttga
     4981 agatgttctg ttcgtgatgg caggccagaa tgacggaacc ggatgccagg aacagcagcg
     5041 ctttaaagaa cgcgtgagtc atcaagtgga aaatcgccgc atcccatgcc tgcacgccaa
     5101 gcgcgaggaa catgtagcca atctggctca tggtagagta agcgagaaca cgtttgatgt
     5161 cggtctgtac cagcgcggca aaaccggcca gcagcagcgt aaccgccccg acaataccca
     5221 ccagatgcag aacttccggc gtcatcagga acaggccgtg ggtacgggcg atcaggtaga
     5281 cacccgcggt taccatcgtt gcggcgtgga tcagcgcgga gacaggcgtc gggcccgcca
     5341 tcgcgtcagc aagccatgtc tgcaacggca actgcgcaga tttaccgacc gcaccgccca
     5401 gcagcatcag cgtcgcccac atcagcatgt tattgccgtc agcaaagtgc gctggtgcca
     5461 gttccaccat atcgcggaag ttcagggtgc ccagttcgtt gtaaagaatg aacagtgcga
     5521 aagcgaggaa aacgtcaccc acacgggtca cgacgaacgc tttcattgct gctgcgccat
     5581 tcttcggatc ggtgtaatag aaaccgatca gcagatagga gcacaggccc acgccttccc
     5641 agccgaggta catcagcagc aggttatcgg cgagaaccag aactaccatg ctggcgatga
     5701 acaggttggt gtaggcgaag aagcgagagt agccctcttc accgcgcatg taccaggagg
     5761 cgtacatgtg aataaggaaa cccacaccag tgaccaccga gagcatggtc agcgacaggc
     5821 cgtccagcac caggttaaaa ccgatgttaa agtcgcctac cgacatccac gtccacagcg
     5881 gctggctgta tgcctgctcg ccgttagcga agaaatcaac gccgataaag gcggttacca
     5941 gcgccgccag gcccacagag ccaacgccga cgatcgccga aacgttttca gaccagcgcc
     6001 cacgggagaa cgccagcagg acgaagccaa tcaatggcaa aataatggtt aaggcaagca
     6061 tgttcatccg cgcatctcac ttactgaatc gatgttcagg ttctggcgac gacggtgaag
     6121 ttgcagcagc agcgcaaggc cgatactcgc ttctgccgcc gcgaggctga tggcgagaat
     6181 gtacatcacc tgaccgtcgg tctgacccca gtagcttccg gcgaccacaa aggccagcgc
     6241 ggaggcgtta atcatgattt ccagaccaat cagcataaac agcagattgc gacggataac
     6301 cagaccggtt aagccaagaa cgaataagat tgccgcgagg atcagtccat gttgtaaggg
     6361 gatcatgcgt gctcctccgt ttttcttttc gcgctgtcgt ctttacgatt gctcagcact
     6421 tcacccgcac gctcttcacg accgacgtgg aaggccacaa ccagacccgc gagcagcagc
     6481 atagaagcca gttccaccgc cagtacgtaa ggcccgaaca gcgtaatacc cactgcttta
     6541 gcactgattg gcgtaccgtc gataccctga tcgttaacac cgaggatggc gtaaacaatc
     6601 accaccagca tgatggccga caaaattgcc gggccaatcc acacctgcgg tttcagccac
     6661 tggcgttcct gttcgatttc tgaaccgccc aggttgagca tcatcaccac gaacacgaac
     6721 agcaccataa tggcacccgc gtagacgata atttccagcg caccggcgaa gtaagcgcct
     6781 agtgagaaga acaccccgga aatcgccagc agcgaaataa tcaggtacag cagtgcgtgt
     6841 accggattgg tatgggtgat cactcgcaag gtcgcaagta tggctatcag gccacagata
     6901 taaaaagcga actccattgc ccctctcctt acggtaacag gctcttgacg tcgataggct
     6961 tggcttcgtt ctctgcttcg cccttatctt tgccgtcgat tgccatacct gccatccggt
     7021 agaagttata ttccgggtat ttgcccggac cggagatcag cagatcctct ttctcgtaaa
     7081 ccagatcctg gcgcttgtat tcccccattt cgaaatccgg ggttaactga atcgccgtgg
     7141 tcggacaggc ttcttcgcac agaccacaga aaatgcagcg tgagaagtta atgcggaaaa
     7201 attccggata ccagcgaccg tctttggttt ctgctttttg cagcgagata cagccgaccg
     7261 ggcaggctac cgcacagagg ttacaggcta cgcaacgctc ttcgccgtcc gggtcgcggg
     7321 tcagaacgat acgaccacga taacggggcg gcagatagac cggctcttcc gggtacattc
     7381 gcgtttcgcg tttggcgaac gcgtgcaggc cgatcatcca gatactacga acctgggtgc
     7441 cgaaacctac taacaattct tttaaggtca tggtcttatt gccccttatt gcgcctgcca
     7501 gagaatgaca gccgccgtta ccagcaagtt gatcagcgtc agcggcaggc agattttcca
     7561 gccgaaggac attacccggt cataacgcgg acgcggtaac gacgcacgaa tcaaaatgaa
     7621 catcatcata aagaacgcgg ttttcagcgc gaaccagatg aatggcggta acaacgggcc
     7681 ttgccagcca ccgaagaaca gcgtcaccat caatgcagag atggtcacaa tcccgatgta
     7741 ctcacccacg aagaacagac cgaacttcat accggaatat tcaatgtggt aaccatccgc
     7801 cagttcctgc tcggcttccg gctggtcaaa cgggtgacgg tgacacaccg ccacgcccgc
     7861 gatggcaaag gtaataaaac caaagaattg cgggatgacg ttccacacat gcgcctggct
     7921 gttgacgatg tcggtcatgt tgaatgaacc ggcctgcgcc accacgccca tcagggaaag
     7981 cccgaggaac acttcgtagc tcagggtctg cgcagaagca cgcatcgcac ccagcagcga
     8041 gtatttgttg ttacttgacc agcccgcaaa cagcaccgcg taaaccgcca gacctgccat
     8101 catcaggaag aacaaaatcc cgatgttcag gtcggcaacc acccaacccg gactgactgg
     8161 cacaattgca aaggccagca gcagcgaggt aaaggcaatc atcggtgcca gggtaaagat
     8221 gacgcgatcc gagaatttcg ggatccagtc ttctttaaag aacattttga tcatgtccgc
     8281 aaccagctgg agcgaaccgc cccagccaac acggttaggt ccataacggt tctggaacag
     8341 acccagcagg cgacgttcgc caaagctcat gaatgcccca caggtgacaa ccaccagcag
     8401 gatcaccacc gctttgagga tggtcagcag gatctcaatc agttccggtg atatccaact
     8461 cattgttgtg cctccttgag atcctcaaga tgcgcgccag ccagcaccgg agcaatgccg
     8521 gacataccca tcggcaagcc cacctgccct gccgtcagtc cttcggcgat ttcaaccggc
     8581 agcgtgaccg tgttgccatc gtaactaaag gagacgcgtg tacctgcgtt cacacccaac
     8641 ttcgcggcat ccgctgggtt gagtttgatg tacggctgcg gcatacggct ctggaagacc
     8701 ggagcacgct gtgacaattc atcgctgcca aacaggtggt agtacggcgc gatacgccat
     8761 ttcccgtcct gcggctggaa gcgtgccggt acgctggtga agtaatccag accgttttcg
     8821 ctggtttcaa acagacgcac gcccggatcg ccaaagcgca gtttgccgcc cacttcgtcc
     8881 tggaatttgt tccacgcctg cggggagttc cagcccggcg cccaggcaaa cggcacttgc
     8941 gaacggtgcg cggtcggctg gttgttacct tccatcgaga aggtgaacat ggtgtcaata
     9001 tcctgcggct gacgcggctc atgaacgctg atattggcgc gcatggcggt acgaccgctg
     9061 taacggtgcg gttcacgggc cagtttctgc ccacgaatac ggaatgtcgc atccggcgca
     9121 gcatctttga tacccgccag ttctgggatt ttcgccacaa cagcgtcaat tacatggtcg
     9181 agctgcgtcc agtccacttc acggctcagc aaggtgctgt gcagcgagtg cagccagcgc
     9241 cagctttcca gcatgacagt tttgctgtcg taataagccg gatcgtaaac ctggaagaaa
     9301 cgttgggcgc gaccttcatt attaattacc gtaccgtcgc tttcagcaaa gctggcagag
     9361 gaaagcacca gatgggcgtt ttccatgatc gctgtgcgtt gatgatcaac caccatcacc
     9421 agcggcgctt tagccagcgc agcattcacg cgggtagcag aagcgtaacg atgcagatcg
     9481 ttttccaaca ccaccaccgc gtcggcgcgt ccggtttcca gttcggttaa cgcttcttca
     9541 agcgaaccgc cacccataat gcccagcccc atgctgttga cggaacgggc aatcatggtg
     9601 ataccaacgt cagcgccgcg acctttcagg gctttcgcga cgttagccgc agcctgaatc
     9661 acctctatgc taccggcgtt cgtcccggag ataatcaacg gtttcttcgc acctgccagt
     9721 gcctgcacga tgacgtcgat tttgttttgc agctcaggtt cgataccgtc aaccgctggt
     9781 gcggagttat ccagcgcatg ggcgatggca aaacctaaac gcgcctgatc ttcaaccggt
     9841 gcgcggtaag tccacgccgc gatatcatcc agacgagtgt catcaacgtt ggtaacaaac
     9901 agcggatgct tcgcacgttg accgatgttg aggattgccg caatctgcca gtcagccact
     9961 ttctgtgctg cagccatttc gcgcgcttta cctttcaccg cctgacgcac tgccagtgcg
    10021 acgcgcgcgc cggtctgggt aacgtcttcg cccagcacca gtaccgcatc gtaagattcg
    10081 atttcgcgca gagccggagt ataaatgccg ccttcacgca gcactttcag cgccagttgc
    10141 agacgttcct gctcaccgtg agcgataccg gtgtagaagt tttcttcgcc caccagttca
    10201 cgcagcgcaa agttgctttc cacgctggca cgcggagaac caataccgat cactttcttc
    10261 gactgacgca gaatatctgc cgcgccctgc attgcctgtt cggcgttgag ggtaatgaaa
    10321 tcatcgccac gacgctgtac tggctgacgc ggacgatcct tcaggttgac gtaaccgtaa
    10381 ccgaaacgac cacggtcgca gaggaagtag tggtttaccg taccgttgta acggttttcg
    10441 atacgacgca gttcgccgta acgttcaccg gggctgatgt tacagccgat ggaacattgc
    10501 tggcagatgc tcggcgcaaa ctgcatatcc catttacggt tgtaacgctc ggagtgcgtt
    10561 ttgtcggtaa atacgccggt cgggcaaatt tcgaccaggt taccggaaaa ttcgctttcc
    10621 agcgtgccgt cttccgggcg accgaagtag acgttgtcgt gcgcgccata aacgcccaga
    10681 tctgtaccgt cagcgtaatc tttgtagtaa cgcacacagc ggtagcaggc gatgcagcgg
    10741 ttcatttcgt gagagatgaa tggccccaaa tcctgattac ggtgggtacg tttggtgaaa
    10801 cggtagcgac ggaagctgtg tccggtcatc acggtcatat cctgaagatg gcagttaccg
    10861 ccttcttcac ataccggaca gtcgtgcggg tggttggtca ttaaccactc gaccacactt
    10921 tcacggaact gtttcgcttc ttcgtcgtca atggaaataa aggtgccatc ggaagccggt
    10981 gtcatacagg acatcaccag gcgaccacgc gtgtcttccg cgttttggta ttgcttcacc
    11041 gcacactggc ggcaagcacc gacgcttccc agcgccggat gccagcaaaa gtaaggaata
    11101 tcaaggccca gagacagaca agcttccagc aggttgtccg ctccgttgac ctcgtattct
    11161 ttgccgtcta catgaattgt agccattagc atgcttccag ttttctcggt cagggaccga
    11221 gcgttaatcg aaattcggtt accagcgctc tttcagcagg ttcggctgaa tcccattaat
    11281 caaatgggta ttgctgaacg gctgtttgat tcccgcctca aattcttcgc ggaaatattt
    11341 gatggcgctc tgtaacggct ccactgcacc aggtgcgtgg gcacagaaag ttttacccgg
    11401 gcctaagaat cgacacagtt gctcaagtgt ttcgatatcg cccggctggc cttcgccacg
    11461 ctccagcgca cgcagaattt tcacgctcca cggcagaccg tcgcggcacg gcgtacacca
    11521 gccgcaggac tcacgggcga aaaactcttc caggttacgc accagcgaca ccatgttgat
    11581 ctcatggtca accgccatcg ccagcgccgt acccagacgg ctgcccgctt taccgatact
    11641 ttcgaattcc atcggcagat caaggtgcgc ttcggtcagg aagtcagtcc ccgcgccgcc
    11701 tggctgccag gctttaaatt tcagaccatc acgcatacca ccggcgtaat cttcgaggat
    11761 ctcgcgtgcg gtggtgccga acggcagttc ccacagtccc ggatttttca cccgaccgga
    11821 gaagcccatc agcttggtgc cagcatcttt acttttcgag atgttctgat accactccac
    11881 gccgttagcg aggatcgccg gaacgttaca cagggtttcg acgttgttga cacaggtcgg
    11941 tttgccccat acgccggagg ttgccgggaa ggggggtttc gagcgtgggt tagcacgacg
    12001 cccttccagg gagttgatta acgctgtttc ttccccgcag atgtagcgcc ctgccccggt
    12061 atggacgaac agttcgaagt cgaaacctgt tcccataatg tttttgccaa gcaagcccgc
    12121 ttcagtggct tcggcaatgg cgcggcgcag atttactgcc gcttcgatat attcgccacg
    12181 caggaagatg tagccacggt aagctttcag cgcaaacgcg gagatgagca taccttccac
    12241 cagcaggtgc ggcagttgct ccatcaacag gcggtcttta taggtgcccg gctccatttc
    12301 atcggcgtta cacagcaggt aacggatgtt catggattcg tctttcggca tcaggctcca
    12361 cttcaggcca gtcgaaaagc ccgcgccgcc gcgccctttc agaccagcgt cttttacctg
    12421 attaacgatt tcgtccggag acagcccggt cagcgcctta cgcgcccctt cgtaaccgtt
    12481 tttgctgcgg tattcgtcca gccacactgg ctgtttgtca tcgcgcagac gccaggtcag
    12541 cggatgcgtt tcgggagtac ggataatgtt tttcatttat accgctccag cagttcaggg
    12601 atcgcttccg gggtcagatg cgcgtgagtg tcctcatcga tcatcatgtt tggcccttta
    12661 tcacagttcc ccaggcagca agttggcagc agcgtaaagc ggccatcaaa tgtcgtttgc
    12721 cctggtttga tgttcagctt tttctcgagc gccgcctgaa taccctgata cccgttgata
    12781 tgacagacca cgctgtcgca ataacggatc acatggcgac caaccggctg gcggaagatc
    12841 tgactgtaga acgttgccac accttcgacg tcgcttgccg gaatacccag cacatcggcg
    12901 atcgcgtgga tcgcaccatc cggcacccag ccacgctgct tctgaacgat tttcagcgct
    12961 tcaatggacg ccgcacgcgg gtcttcgtag tggtgcatct cgtgctcaat cgcttcacgc
    13021 tctgccgcac tcagctcaaa agcctcggtt tgtggttgtt gattctcgtg cataattagc
    13081 ggtccacatc tgacataaca aaatcgatac tgcccagata aacaatcagg tcagacacca
    13141 ggctgccgcg gatcgccgcc ggaatttgct gcaaatgcgc ataactcggc gtgcggatac
    13201 gagtgcgata actcatggtg ctaccgtcgc tggtcaggta gtaactgttg atccccttgg
    13261 tcgcctcaat catctggaaa gattcattgg caggcatcac cggcccccac gacacttgca
    13321 ggaagtgggt gatcagggtt tcgatatgtt gcagcgtgcg ctctttcggc ggcggcgtgg
    13381 tcagcgggtg atccgctttg aacgggcctt ccggcatgtt gttgaggcac tgctcaagaa
    13441 tgcgcagact ctggcgcagc tcttccactt tcagcattac gcgggtgtag cagtcagaaa
    13501 caccaccacc aaccgggatt tcaaagtcga agttttcata gccagaataa ggacgcgcct
    13561 tacgcacgtc gaagtcgatc ccggtagcac gcaggcccgc gccagtggtg ccccactcca
    13621 gcgcctcttt cgcgccatag gcggcaacgc cctgggaacg acctttcaga atggtatttt
    13681 gcagcgccgc tttctcgtaa gacgccagac gtttcggcat ccagtcgagg aactcacgca
    13741 gcagacgatc ccagccgcgc ggcaggtcgt gcgctacgcc gccaatacgg aaccacgccg
    13801 ggtgcatacg gaaacccgtg atcgcttcca ccagatcgta aattttctga cgatcggtaa
    13861 aggcgaagaa cacgggcgtc attgcgccga cgtcctgaat aaaggtcgag atgtacagca
    13921 ggtggctgtt gatacggaac agttcggaga gcataacgcg aatgacgtta acgcgatccg
    13981 gcacggtgat cccggccagt ttctctaccg ccagcacgta aggcatttcg ttaacgcagc
    14041 cgccgaggta ttcaatacgg tcggtatacg gaatgtagct gtgccaggac tggcgctcgc
    14101 ccattttctc cgcaccacgg tggtggtagc cgatgtctgg tacgcagtcg acaatctctt
    14161 cgccgtcgag ttgtaaaacg atacggaaag ccccgtgcgc cgacgggtgg ttcggaccga
    14221 ggttgaggaa catgaagtcc tcattttcgg tgccgcgctt catcccccac tcttccggtt
    14281 tgaaggtcag ggcttccatc tccagatcct gtttggcttt ggtcagctca aacggcgaga
    14341 attcggtagc gcgtgccggg taatctttac gcagcgggtg acctttccag gtttgcggca
    14401 tcatgatgcg gcgcaggttc gggtgaccgt cgaaagtaat gccaaacaga tcccaggttt
    14461 cacgctcata ccagttagcg ttcgggaaca gtttggtgaa ggtcggtacg tgcaggtcgt
    14521 tttctgccag cgccaccttc agcatgatgt cgcggttacg atcgatagaa atcagatggt
    14581 agaaaacgga aaaatccgcg gcaggtaacc cttcgcggtg tgtgcgcaga cgttcgtcca
    14641 tgccgtgtaa gtcaaacagc atgacgtaag gtttcggcag tttctttaag aaatcgccaa
    14701 cttccagtaa ttgttcacgc ttgatccaca caacgggaac cccggtgcga gtcgcctgaa
    14761 cagtaaaggc atccggccca aaacggttgc gcagttcgcc aattaccgga tcatcaagat
    14821 gatcgcgggt ctgccaggcg ggttcttgcg cggttaagtc ggtcatattg ttcaccattg
    14881 caaatggtcc gtggtgactg tcggcgcagt aagcttcgcg aaatagattt aggaataagc
    14941 gaagaaaaat ccctttgccg acaggcgcaa attaaatctc gtcaggtgta cgcaggttgg
    15001 ttacggcaat gcgttcaccg cgcttgcgtt cgcgctctga ttgcatattg gcgcgataaa
    15061 cgccctgatc gccaaccacc caggagagcg gacgacgttc tttgccgata gattcctgca
    15121 acagcatcag tgcctgcatg tacgcttcag gacgcggcgg gcagcccggg atatacacat
    15181 caaccgggat gaatttatcg acgccctgca caacggaata aatatcgtac ataccaccag
    15241 agttggcaca ggcacccatt gagataaccc attttggttc cagcatctgg tcatacagac
    15301 gctgaataac cggtgccatt ttggtaaagc aggttcctgc aaccaccatc aggtcagcct
    15361 gacgcggcga agcacgcaat acttctgcgc caaaacgcgc cacgtcatgc accgcggtaa
    15421 acgaagtcac catctcaacg taacagcagg aaagaccgaa gttatacggc caaattgagt
    15481 ttttacgacc ccagttaacc atgtcattga gcttgcccat aaacacgttt ttgttaactt
    15541 cttgctccag agggtcggtt acgatctcct gcttttgcag ggggtaacgg tcgttctcac
    15601 cgttgggatc tatgcgggtg agcgtataat ccatcttaat gcctcgcggt tagcgttgac
    15661 gattagcgat actgttcgtt tccgggttca tacgctcgcg gcgtgaacgc gcgggcgtcc
    15721 agtccagcgc gccaatacgc accagataaa ccagacctgc cagtaacaca aaaataaaaa
    15781 ttgcagcttc cacaaagcct acccagccgc tttcgcggat agaggttgac catgcgaaca
    15841 gatacagcgc ttcaacgtcg aagataacga agaacatggc caccagataa aacttggcgg
    15901 acaggcgtaa gcgggcggag ccgaccgagt cgataccgga ttcaaacggc acgtttttcg
    15961 acctcgcgcg tgcgcgaccg cctaaaaacc aaccgcctac cagcatcagg caacacaggc
    16021 caatggcaac gataagaaag atagcgaatg cccagtgatg agcgatgact tcagtggatg
    16081 ttgacatact cattgcttac tcatcaaaag tagcgccaga ttcactgctc ttcacggcag
    16141 atggacgcca catcgattca tggggaggaa taaaaaaaac cttacaataa ctgtagaaat
    16201 tcttttatac agctaattga tgtggtcttt tactcctttc tataaccttt tgtcaacttt
    16261 aacaaaagct tcttcacatt agtttacata atatcaacac cattagcatt taatgccctt
    16321 tcaccccaga tccttgacga ctccaggata attagatgtt gttgaatcgt gtccgttgtg
    16381 aagcaatgga aaaaatacgg gtctattttg acaggaattt gtgtcgattc ctccccccaa
    16441 aagagagtat tttcttgatc tgtgacacgc ttttgttatt ccataacaaa aacgcagcaa
    16501 caaatttacg tattttttaa catcattgta gcaggtgatt tttttcaggc gattatttgt
    16561 gcattcggga cgtgaatctc tggtgattga aaaataaaca gttttgtacg ttctgcacta
    16621 tgcgaggaag gatttttact aaaaaaaagc cgctggggtt taaaacaccc ccagcggctc
    16681 gttttttaca ctattgtctc aggaattatc tatcgtccgt cgattactcg atatcccttt
    16741 caatcaacaa tgaatcatcc ccttccggag cagacattgg gctgtattgc cacggattat
    16801 ggtagctctc catcgcctga tagatcacct gggccagctc attattactg gacgggtcat
    16861 agcacagcaa atattcggta tcaggtagcg gcggtaagcc atctactcca ctcagcacac
    16921 gcaggtccgg gctcatcatc tcaaccggcc ttgccgtcac gccaagaccg gctttcactg
    16981 ccgcacgaac ggccggaagc gtcgaggcga cataagccag tcgccatgga atatctactt
    17041 tattaagcgt cgccagcacc atatcgcgaa acggacttgg atcatccagc aatacaagag
    17101 ggatcggctc gcctttttgc aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg
    17161 tacgcaggtt caatgcctta aacgctgaag gacgatgggt ggttaccatc aaatccactt
    17221 cctgggattc cagcatttct gccatatagg cattacgttt aacgcggaca tccagcgcta
    17281 atttcggata aaccgaactc acgcgattta acaggaaagg taagatcgta tcggcagatt
    17341 catctgaagc accgatagtt aacacgccct gaagattact gaacattaat gatgagcagg
    17401 cctcatcatt aaaacgcagg attttcctgg cgtaaccaag aagttgaatg ccatgttcag
    17461 ttaacagttt gttgcgaccg tgacgagcga acagttcttt cccaacgagt tgttccagac
    17521 gctgcatttg ctgacttacg gcggactgag tacgacacac agcggcagct gcggcagcaa
    17581 aagtgttcag atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg aggttaatta
    17641 tcggacgatt tgcacttatc atatattatc acttactggc ggctcatact gagctggtta
    17701 atgctgtgca cacacaaaca agcaattcca tttgtaatgt gcctccctgg cagtttcatc
    17761 cggaacccgg acgaaagtaa aaatgcatat gagttgcact aaaaaagcga ctcacattgt
    17821 tccgttataa tgcctgaagt agatcacaga atatatcttc agggatcgca tatctattaa
    17881 gttactcact cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc
    17941 ttgttccact ctcgtgcagc atcgctggtc atacgcgaac acgtacaaac agcaatggtg
    18001 aagcggcatc aagagcaggg atccgtcatt tatgcgagca ttttacccca aaaacctttt
    18061 atttataagg gtcattgcga attatctgat gcaaagttat gttatgttag gcaaagtaat
    18121 cttctattat taataagcac atcaaaacct ttttgaatat taaataataa ttaattagca
    18181 tcatcctcat tcattaattc cgcttaacaa tagtttcaca ccttaccccc attgcgacac
    18241 ctcggaaatc atcacgctgt taaactctcc gtaatgcgga aatatcatca tcgcgtttca
    18301 ttagtgaatt cttaaatgag gcattttcac acaattatct tacagataaa aaaaccagac
    18361 ttgcaattaa gaattagaac aattcactat ataacattgc atgtaaagca tataagcctc
    18421 attatttgtc attattaagt tattaacagc acaatcgagc cttcccctct ggcaaaatct
    18481 tattctgcag accttcaaaa caccgtcctg ggggagtaca ttgttctaag ctgacttcca
    18541 cggcagggag tggcgataac agcaaaaaag gtcaagattc atgtccccca ttgaaaaatc
    18601 cagcaaatta gagaatgtct gttatgacat ccgtggtccg gtgctgaaag aagcaaaacg
    18661 cctggaagaa gaaggtaaca aggtactgaa actgaacatc ggcaacccag ccccattcgg
    18721 ttttgacgcg ccagatgaaa tcctcgttga cgtgatacgc aacctgccta ccgctcaagg
    18781 gtattgcgat tccaaagggc tttactccgc gcgtaaagcc atcatgcagc actaccaggc
    18841 tcgtggcatg cgtgatgtta ccgtggaaga tatttacatc ggcaatggtg tatcggagct
    18901 tatcgttcag gcaatgcagg cattgctgaa cagcggggac gaaatgttgg ttcctgcacc
    18961 agattaccca ctctggaccg cggcggtttc gctttccagc ggtaaagcgg tgcattatct
    19021 ttgcgatgaa tcctctgact ggttcccgga cctcgatgat attcgcgcta aaattacgcc
    19081 tcgtacgcgt gggattgtta ttatcaaccc aaataaccca accggcgcgg tatattccaa
    19141 agagctttta atggagattg tggagattgc acgtcagcat aatctcatta tcttcgccga
    19201 tgaaatttat gacaaaattc tctacgacga tgctgagcat cactcaattg cgccgctggc
    19261 acctgacctg ctgaccatta cctttaacgg actgtcgaaa acgtaccgcg ttgcaggctt
    19321 ccgtcagggg tggatggtgt tgaacgggcc gaaaaaacac gccaaaggct acatcgaagg
    19381 tctggaaatg ctggcttcaa tgcgcctgtg tgctaacgtt cctgcgcaac acgccattca
    19441 gaccgcgctg ggtggttatc agagcatcag tgaatttatt acccctggcg gtcgtcttta
    19501 tgagcagcgt aaccgcgcgt gggaactgat caacgatatt ccgggcgttt cctgcgtgaa
    19561 acctcgtggt gcgctgtata tgttcccgaa aatcgacgcc aaacgcttta acattcatga
    19621 cgatcagaaa atggtgctgg atttcctgtt gcaggaaaaa gttctgttgg tgcaagggac
    19681 ggcattcaac tggccgtggc cggatcactt ccgcattgtc acgctaccgc gtgtcgatga
    19741 tatcgagctg tctttgagca agttcgcgcg tttcctttct ggttatcatc agctgtaatc
    19801 ttaatttcac tgccggagat tgcatccggc agtgttatcc cgccacaatg acctgatgat
    19861 gtcatcatac gtaaggtcac tatgaaacag agccattttt ttgcccatct ctcccgcctg
    19921 aaactcatta accgctggcc gctaatgcgc aacgtgcgga cggaaaatgt gtccgaacac
    19981 agtttgcagg tagcgatggt cgcccatgcg ctggcagcta tcaaaaatcg gaaatttggc
    20041 ggtaatgtca acgccgaacg tatcgcttta ctggcgatgt accacgatgc ctcagaagtg
    20101 ctcaccggcg atctccctac tccggtgaaa tacttcaatg cgcaaatcgc tcaggagtac
    20161 aaggctattg aaaaaatcgc tcagcagaaa ctggtcgata tggttccgga agaactgcgg
    20221 gatatctttg cgccgttaat tgacgagcat gcatatagcg atgaagaaaa atcgctggtg
    20281 aaacaggcag atgcactgtg tgcatatctg aaatgtctgg aagaactcgc ggccggaaat
    20341 aatgaattct tgctggcaaa aacgcgactg gaagcgacgc ttgaagcgcg tcgcagccag
    20401 gagatggact acttcatgga agtatttgtt cccagcttcc atctttcgct cgatgagatt
    20461 agccaggatt caccgctgta agcagccgga gtctgcgtcg catcagtcaa taagcgccgg
    20521 atgcgacatc aggctcttgt caaaacggaa acagcatcgg gatcatcacc acacaaaccg
    20581 ccatcacgat aatggtgaac ggtaccccca acttcacaaa gtcactgaag ctgtaatttc
    20641 ccggacctaa aaccagggtg ttaacaggtg aagacaccgg tgtcataaag gcggcggatg
    20701 ctgccatcgc cacgaccatc gcaaatggat aaggcgacac tcccatcgtt ttggcagcag
    20761 ccagcgcaat cggagccatc aacaccgccg tcgcggtatt agagataaac agcccaataa
    20821 ccgccgacaa gaca
//
LOCUS       DAZSNM010000057        20826 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZSNM010000057 DAZSNM010000000
VERSION     DAZSNM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20826
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.057"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..257)
                     /locus_tag="ACHW42_001742"
     CDS             complement(<1..257)
                     /locus_tag="ACHW42_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR8943595.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETD"
     gene            complement(435..2084)
                     /gene="actP"
                     /locus_tag="ACHW42_001743"
     CDS             complement(435..2084)
                     /gene="actP"
                     /locus_tag="ACHW42_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR8943596.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(2081..2395)
                     /locus_tag="ACHW42_001744"
     CDS             complement(2081..2395)
                     /locus_tag="ACHW42_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFR8943597.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2595..4553)
                     /gene="acs"
                     /locus_tag="ACHW42_001745"
                     /pseudo
     CDS             complement(2595..4553)
                     /gene="acs"
                     /locus_tag="ACHW42_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4946..6382
                     /gene="nrfA"
                     /locus_tag="ACHW42_001746"
     CDS             4946..6382
                     /gene="nrfA"
                     /locus_tag="ACHW42_001746"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR8943598.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6427..6993
                     /gene="nrfB"
                     /locus_tag="ACHW42_001747"
     CDS             6427..6993
                     /gene="nrfB"
                     /locus_tag="ACHW42_001747"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR8943599.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6990..7661
                     /gene="nrfC"
                     /locus_tag="ACHW42_001748"
     CDS             6990..7661
                     /gene="nrfC"
                     /locus_tag="ACHW42_001748"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR8943600.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7658..8614
                     /gene="nrfD"
                     /locus_tag="ACHW42_001749"
     CDS             7658..8614
                     /gene="nrfD"
                     /locus_tag="ACHW42_001749"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR8943601.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8634..10352
                     /locus_tag="ACHW42_001750"
     CDS             8634..10352
                     /locus_tag="ACHW42_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR8943602.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10345..10728
                     /gene="nrfF"
                     /locus_tag="ACHW42_001751"
     CDS             10345..10728
                     /gene="nrfF"
                     /locus_tag="ACHW42_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR8943603.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10725..11321
                     /gene="nrfG"
                     /locus_tag="ACHW42_001752"
     CDS             10725..11321
                     /gene="nrfG"
                     /locus_tag="ACHW42_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR8943604.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11663..12976
                     /gene="gltP"
                     /locus_tag="ACHW42_001753"
     CDS             11663..12976
                     /gene="gltP"
                     /locus_tag="ACHW42_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR8943605.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(13054..13743)
                     /gene="yjcO"
                     /locus_tag="ACHW42_001754"
     CDS             complement(13054..13743)
                     /gene="yjcO"
                     /locus_tag="ACHW42_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR8943606.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13837..15984)
                     /gene="fdhF"
                     /locus_tag="ACHW42_001755"
                     /pseudo
     CDS             complement(13837..15984)
                     /gene="fdhF"
                     /locus_tag="ACHW42_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(16182..17648)
                     /gene="mdtP"
                     /locus_tag="ACHW42_001756"
     CDS             complement(16182..17648)
                     /gene="mdtP"
                     /locus_tag="ACHW42_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFR8943607.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17645..19687)
                     /gene="mdtO"
                     /locus_tag="ACHW42_001757"
     CDS             complement(17645..19687)
                     /gene="mdtO"
                     /locus_tag="ACHW42_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR8943608.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            complement(19687..20718)
                     /gene="mdtN"
                     /locus_tag="ACHW42_001758"
     CDS             complement(19687..20718)
                     /gene="mdtN"
                     /locus_tag="ACHW42_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR8943609.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20737..>20826)
                     /locus_tag="ACHW42_001759"
     CDS             complement(20737..>20826)
                     /locus_tag="ACHW42_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8943610.1"
                     /translation="RANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4675 a         5480 c         5684 g         4987 t
ORIGIN      
        1 ctgtccgttt ctttcgtgat ttttcctgat ggtaaactat cataaattct tttcattttc
       61 tgaatcaagc ctatcacttt ttcatgatca tctattttga aaagagagag ggctttattg
      121 tcaattaacg tgtttttatg atgtttgctg taattatctg attttgtttt ctttgtatgt
      181 gatgaacttg tgatagagtg gttatcgtta atctggcaaa gagggaaaaa catattattt
      241 aaaccattat agcgcataaa atatccttat aattaacaag aaagaaaagg catattctct
      301 gcattatcat tttctacgac tgtcaaaaat cgctcatttt ttaatgagtt tatttgttta
      361 atattatggg aaaaggtgat gcatgttgga gaggaagcgt attccccggt caggcgaccg
      421 gggaagggga gagattaatg tgcgcggcct tgctcaacgc caaagccggt ctgggagcgg
      481 ataaactgcg cgcggaacag ttcacgctcg cgcgcccctt ccgctgagtt atcggttgcc
      541 gagaagaacc agatgccgag gaatgccacg ctgatagaga acagcgccgg gtattcatac
      601 gggaagatgg ctttttcgtg accgaggatc tgtacccaaa tagtcgggcc gaggatcatc
      661 agcaccactg cggtaatcag ccccagccag ccgcccagca tcgcgccacg ggtagtcagt
      721 ttcgaccagt acatggaaag cagaatgatc gggaagttac agctcgccgc gatggcaaac
      781 gcaaggccta ccataaaggc gatgttctga ttctcaaaca gcacgccgag gataatcgca
      841 atcacgccga ggatcagtac ggtgattttt gatacccgca gctcttcacg ttcggtcgcg
      901 ccttttttga agacgttagc gtacaagtca tgcgaaaccg ccgatgcgcc cgccagcgtc
      961 agatccgcaa ccaccgcgag gatagtggcg aaagcaaccg ctgaaataaa accgaggaat
     1021 aggttgccgc ccactgcatt cgccaggtga acggccgcca tgttgttacc accaatcaga
     1081 tggcccgccg cgtctttata ttccggattc gcaccaacaa gcatgatcgc gccgaagccg
     1141 ataataaagg tcagaatata gaagtagccc ataaatccgg tggcgtagaa cacgctctta
     1201 cgtgcttcgc gggcatcgct gactgtaaag aagcgcatca gaatgtgcgg caagcccgcc
     1261 gtaccaaaca tcagtcccag ccccagagag agcgcggaga tcggatcttt caccagtccg
     1321 cccggtttca tgatgtcgac acctttcggg tgtaccgcca tcgcttcgct gaacagattg
     1381 ttgaagctaa agccgacgtg tttcatcacc ataaaggcca taaagctggc accgaacagc
     1441 aacagcacag ctttgataat ttgtacccag gtggtcgcca gcatgccgcc gaacaggacg
     1501 tacatcatca tcagcacgcc gaccagcacc accgcaatgt gatagttaag gccaaacagc
     1561 agctcgatca gtttacctgc gcctaccatc tgagcgataa ggtaaagcgc caccactacc
     1621 agagaaccac aggccgaaag aatacgaatc ggcccttgtt tcagacgata agaggccaca
     1681 tcggcagagg tgtagcgtcc caggttacgc agacgttcgg caatcagaaa cagaatgatt
     1741 ggccagccca ccaggaagcc caacgagtag atcagcccgt catagccgga ggtaaacacc
     1801 agcgcggaga tccccaagaa tgaggcggcg gacatatagt ccccggcaat cgccagcccg
     1861 ttctggaagc cagtgatatt gccgcctgcg gtgtagtagt cgctacgaga acgtacgcgt
     1921 tttgatgccc agtaggtaat gccgagcgta aacacgacga aaatcaggaa cataataatc
     1981 gcctgccagt tcgttggctg gcgctctacg gccccgctaa tagcatccgc ggcgttagct
     2041 gcgaaaggga gtgtggcggc aagcgccgtc agaactcttt tcatgatgct tgtacctcat
     2101 gcaggacttc attattaaga cggtcgaatt cgccgttcgc ccgccagatg tagataccgg
     2161 tgagaacaaa ggagatcaca atcactccaa caccaattgg aataccgcgt gtgacgctgg
     2221 tgttcggatt cagcggcgtg cccagccagc cgggcgcgaa ggcgatcagt aaaataaagc
     2281 cgatataaac tgccagcata ataatcgaca ggatggtggc aaaccgttgc cgtttttcga
     2341 ctaactccct gaaatgcgca ttgtcttcta tccgctgata aatcgtacca ttcatcacag
     2401 attctccaga ggtaatgtag ggattgtttt aattcccgct cccttatggg agaaggttaa
     2461 cgctcgggta acccttgccg aatgtaggcc ggataaggcg tttacgccgc atccggcaat
     2521 caatgcctga tgcgacgctg tcgcgtctta tcaggcctac aaaccgttac cgattcgcat
     2581 ccggcaattg tgggttacga tggcatcgcg atagcctgct tctcttcaag cagcttctcg
     2641 accacgccag gatcggcaag cgtcgaggta tcgcccaggt tgctggtatc gcccgccgca
     2701 attttgcgca gaatacggcg cataattttg ccggagcggg ttttaggcag ggagtcggtc
     2761 cagtgcagca cgtctggcgt cgccagcggg ccaatctctt tacgcaccca gttgcggact
     2821 tctgcgtaca gttctggtga cggttcctcc ccgtgattaa gcgtgacata ggcgtagatc
     2881 gcctgacctt taatattgtg cggaatacct actacggcgg cttcggcaat cttcggatgc
     2941 gccaccagcg ccgactctat ctctgccgtc cccagacggt gaccggagac gttcagcacg
     3001 tcgtccacac gcccggtgat ccagtaatag ccatcttcat cgcgacgcgc gccgtcgccg
     3061 ctgaaataca tatttttgaa ggtggaaaaa taggtctgct caaaacgttc gtgatcgcca
     3121 aacagcgtac gcgcctgacc aggccaggag tcggtgatca ccaggctacc ttcggtagcc
     3181 ccttccagcg ggttaccttc gttatcgacc agcgccggtt gcacgccgaa gaacggacgt
     3241 gttgccgaac cggctttcag ctcggtagcg ccaggcagcg gggtgatcat gaaaccgccg
     3301 gtttcggtct gccaccaggt atcgaccacc ggacatttct cgttgccgat ttttttccag
     3361 taccactccc acgcttccgg gttaattggc tcgcccacgg aaccgagaat gcgtagcgac
     3421 gaacggtcgg tgccttcaat cgctttatcg ccttccgcca tcagcgcgcg gatcgccgtg
     3481 ggtgcggtat agagaatatt gacctgatgc ttgtccacca cctgcgccat acgggcaggc
     3541 gtcggccagt tcggtacgcc ttcaaacatc agcgtggttg cgccgcaggc cagcgggccg
     3601 tacagcaaat aactgtgtcc ggtaacccag cccacatcgg cggtgcacca gtagatatca
     3661 cccggatgat aatcaaagac atatttaaag gtcagcgccg cgtacaccag ataaccgccg
     3721 gtagtgtgca gtacgccttt tggttttccg gtagaaccgg aggtatagag aataaacagc
     3781 ggatcttcgg cgttcatctc ttccgcctag tgctgatcgc tggcttgctc aaccaggtcg
     3841 tgccaccaca ggtcgcgccc ttcctgccag tcgatgtttc cgccagtacg cttcagtacc
     3901 accacatgct ctacgctggt gacgttcggg ttttttagtg cgtcatcaac gtttttcttc
     3961 agcggaatac tacgcccggc gcgcacgcct tcgtcggaag tgatcaccag tcgtgagttg
     4021 gaatcaataa tgcgcccggc aacggcttcc ggcgagaagc cgccgaaaat caccgaatgc
     4081 atcgcgccaa tgcgggcgca ggccagcatc gcaaccgcgg cttccggcac catcggcata
     4141 taaatcgcca ccacatcacc ttttttaatg cccagctcga gcagggtatt ggcgaagcgg
     4201 cagacgtcgc ggtgcagctc tttatagctg atatgtttgc tctggctggc gtcgtcgcct
     4261 tcccagatga tggcggtgcg atcgccgttt tcttgcagat ggcggtcaag gcagtttgcc
     4321 gccagattca gcgtgccgtc ctcgtaccat ttaatggaca cattaccggg ggcaaaggag
     4381 gtatttttca ccttctggta cggtttgatc cagtcgagaa tttttccctg ttcgccccag
     4441 aaggtatcag gtacgttaat agattgttga tacatcgcct cgtactgctg agggtttatc
     4501 aggcaacggt ctgcgatgtt ggcaggaatg gtgtgtttgt gaatttggct catgcttttg
     4561 ttctccttgt aggatgttaa taatatgtgg cataagcgtt aaatgtaggg gtattggcag
     4621 ttttgtttag tatttgggcg acagatcacg caaaagtaga atggtgcaaa taaacggcag
     4681 ggtaattttt gaaggtcacc aacaaaagtt gattaattct ttgaggaaca tgcagttatg
     4741 catgctgttg aaaaagagga agatactgac taactctaaa gtggtatttc acatgcactt
     4801 acaattgatt aaagacaaca ttcacagtgt ggttatttgt tacacatagg ggtgagcaat
     4861 gtcatgacag tgtaggtgcg gttactgtcg tgaaaaacaa taaaaaccgc cattgcaaca
     4921 atggcgcaat tcggatgaag cccctatgac aaggataaaa ataaacgcac gccgtatctt
     4981 cagcttattg attccttttt tctttttcac ttctgttcac gctgaacaaa cggcagctcc
     5041 cgcaaaacct gtaactgtgg aagcgaagaa tgaaaccttt gccccgcagc atcacgatca
     5101 atatctctcc tggaaagcca cctcggaaca gtcagagcgt gttgacgccc tggcggaaga
     5161 tccacggctg gtgatcctgt gggcggggta tcccttctcg cgcgattaca acaagcctcg
     5221 tggacatgcc tttgctgtga ccgatgtgcg tgaaaccctg cgtaccggtg cgccgaaaaa
     5281 cgctgaagat ggtccgctac cgatggcgtg ctggagttgt aaaagcccgg atgtggcgcg
     5341 tctgatccag aaagacggcg aagatggcta cttccacggt aaatgggcgc gcggcggccc
     5401 ggaaatcgtc aacaacttag gttgtgccga ctgccataac accgcctcac cagagttcgc
     5461 caaaggcaaa ccggagttaa ccctttcccg tccgtatgcg gctcgcgcga tggaagccat
     5521 tggtaaacct tttgagaaag ccggacgttt cgaccagcaa tcgatggttt gcggtcagtg
     5581 ccatgtggag tattacttcg acggcaaaaa caaagcggtt aaattcccgt gggatgacgg
     5641 catgaaagtc gaaaatatgg agcagtatta cgacaaaatt gccttctctg actggactaa
     5701 ctccctgtcg aaaacgccaa tgctgaaagc gcagcacccg gaatatgaaa cctggacagc
     5761 gggcattcac ggtaaaaaca acgtgacctg tatcgactgc catatgccaa aagtgcagaa
     5821 cgccgaaggc aaactctaca ccgaccataa aattggtaat ccgtttgata acttcgccca
     5881 gacttgtgcg aactgccata cccaggataa agctgccttg caaaaagtgg tcgcggaacg
     5941 taagcaatcg attaacgacc tgaaaatcaa ggttgaagat caactggttc acgctcactt
     6001 cgaagcgaaa gcggcgctgg atgcaggcgc gacggaagcc gaaatgaagc cgattcagga
     6061 cgatatccgt catgcccagt ggcgttggga tctggcgatt gcttcccacg gtattcatat
     6121 gcacgcaccg gaagaaggtc tacggatgct cggtacggcg atggataaag cggcggatgc
     6181 gcgcaccaaa ctggcgcgcc tgctggcgac caaaggtatc acccatgaaa tccagatccc
     6241 ggatatctcg accaaagaga aagcccagca ggccattggc ctgaacatgg aacaaatcaa
     6301 ggccgagaag caggacttca tcaaaacggt gatcccgcag tgggaagagc aggcacgtaa
     6361 aaacggtctg ttaagccaat aaccccgttc cgcctcgcaa ggggcggaaa acacaatgga
     6421 gtgaatatga gcgtattacg ttcgttatta actgctgggg tgctggcgtc gggcctgttg
     6481 tggagcctga acggaattac cgctacccct gcggtgcagg catctggtga tcgctacgaa
     6541 gttacccagc agcgtaaccc ggatgccgcc tgtctggact gtcataaacc agataccgaa
     6601 ggtatgcatg gcaaacatgc ttccgttatc aacccgaata acaaattgcc ggtcacctgc
     6661 accaactgtc acggccaagc atcaccgcaa caccgcgaag gggtgaaaga tgtgatgcgc
     6721 tttaacgagc cgatgtataa ggtcggagag cagaacagcg tctgtatgtc ctgtcatctg
     6781 ccggaacagt tgcaaaaagc gttctggccg cacgatgtcc acgtaaccaa agtggcgtgc
     6841 gccagctgcc attccctgca tccacagcaa gatacgatgc agacgttaag tgacaaagga
     6901 cggattaaga tttgcgtcga ttgccacagc gatcagcgca ccaatccgaa ctttaacccg
     6961 gcgtctgttc cgttgcttaa ggagcagcca tgacctggtc tcgtcgccag tttctcaccg
     7021 gcgtcggcgt gctggcagcc gtcagcggca ccgcagggcg cgtggtggcg aaaacgttga
     7081 atatcaatgg ggtgcgttac ggcatggtgc atgacgagtc gttatgcatc ggctgtaccg
     7141 cctgtatgga tgcttgtcgg gaagtgaaca aggtgccgga aggcgtctcg cgcctgacga
     7201 ttattcgtag cgagccgcag ggcgaatttc ctgatgtgaa atatcgcttc ttccgtaagt
     7261 cttgccagca ctgcgatcat gcgccgtgcg ttgacgtctg cccgaccggt gcgtcttttc
     7321 gtgatgccgc tagcgggatt gtcgatgtta atcctgatct ctgcgtcggt tgtcagtact
     7381 gcatcgccgc ctgtccgtac cgcgtgcgct ttatccatcc ggtcacgaag acggcggaca
     7441 aatgcgattt ctgtcgtaag accaatttgc aggccggtaa gcttcccgcg tgcgttgaag
     7501 cttgcccgac gaaggcgctg acgtttggca atctggacga tcctaacagt gagatttcgc
     7561 aactgctgcg ccagaagcct acttaccgct acaagctggc gctgggaacc aaaccgaagc
     7621 tgtaccgcgt accgtttaaa tacggggagg tgagccaatg acgcagactt ccgcatttca
     7681 ttttgaatcg ctggtgtggg actggccgat tgccatctac ctgtttttga ttggtatttc
     7741 tgccggtctg gtgacgctgg ccgtgctgtt acgtcgcttc tacccgcagg cgggcggtgc
     7801 agacagtacg ttgctgcgca ccacgctgat tgtcgggccg ggcgcggtga tcctcggtct
     7861 gttgatcctc gtcttccacc tgacaagacc gtggaccttc tggaagctga tgttccacta
     7921 cagttttacc tcggtgatgt cgatgggggt gatgctgttt cagctctaca tggtggtgct
     7981 ggtgctgtgg ctggcgaaaa tctttgaaca tgatttgctt gccctgcaac aacgctggtt
     8041 gccgaagctg gggatcgtgc aaaaggttct gagcctgctg acgcccgttc atcgcggact
     8101 ggaaacgctg atgctggtgt tggcggtgct gctgggggct tataccggct ttctgctgtc
     8161 ggcgctgaaa tcgtatccgt tcctcaataa cccgagcctg ccggtgctgc tcctcttctc
     8221 cggcatctcg tccggtgcgg cggtggcgct gatcgccatg gcgatacgcc aacgcagtaa
     8281 cccgcattcc acggaagcgc actttgttca ccggatggaa atccccgtgg tatggggtga
     8341 aatcttcctg ctggtggcgt tttttgtcgg tctggcgctg ggcgatgacg gtaaagttcg
     8401 tgcgctggtg gcggcattag gtggcggttt ctggacgtgg tggttctggc ttggtgtcgc
     8461 cgggctgggg ctgattgtgc caatgttgct caaaccgtgg gtcaatcgca gttccggcat
     8521 tcctgccgtg ctggcggcgt gtggggccag tctggtcggc gtgttgatgc tgcgcttttt
     8581 cattctctac gccggacagt tgacggtggc gtaagccaga aaagaggtgg tttctggacg
     8641 tattccttcc tgaagtcggt tttctggcgt tgttgttaag tctcggggtc aacgtgttga
     8701 ccccgttgac ggcctttgcg ggagtgcggt tgcgctggcc tgccatgatg cgactcactt
     8761 gcatcggcat tctggcgcag ttcgcgctcc tgctgctcgc ctttggcgta ctgacgtatt
     8821 gttttctcat cagcgatttc tcggtcattt atgtcgccca acatagctac agcctgctgt
     8881 cgtgggaact caaactggcg gcggtgtggg gcggtcatga aggttcgctg ctgctttggg
     8941 tgctgctgct ttccgcctgg agcgcgctgt ttgcctggca ttatcggcag caaagcgatc
     9001 cgctatttcc gctgacgcta gccgttttat ctctcatgct cgccgcactg ctactgtttg
     9061 tggtgctgtg gtccgatccc ttcgtgcgga tatttccacc agcaatcgaa ggccgcgatc
     9121 tcaatccgat gctgcaacat cccggtctta tctttcatcc accgctgctt taccttggct
     9181 atggcggttt gatggtagcg gcgagcgtgg cgctggcgag tctactgcgc ggcgagtttg
     9241 atgcggccag cgcccgaatt tgctggcgct gggcgttacc tggctggagt gcattaacgg
     9301 cggggatcat cctcggttcc tggtgggcct actgcgaact cggctggggc ggctggtggt
     9361 tctgggatcc ggtggaaaac gcctctttat taccctggct ttctgccact gcgctgctgc
     9421 acagtttgtc cctgacacgc cagcggggga ttttccgcca ctggtcgctg ttgctggcga
     9481 tagttactct gatgctgtcg ctgctgggca ccttaattgt ccgttctggc attctggttt
     9541 cggttcatgc gttcgcactg gataacgtcc gcgccgtgcc gttgttcagc ctgtttgcac
     9601 tgattagcct tgcgtctttg gctctgtatg gctggcgagc gcgggacggt ggcccggcgg
     9661 tgcgtttttc ggggttatcg cgggaaatgt taatcctcgc tacgctgttg ctgttttgcg
     9721 cagtgctact gatcgtgctg gtgggaacgc tttatccgat gatttacggc ctgctgggct
     9781 ggggacgcct ctccgttggc gcgccgtatt ttaaccgcgc gacgttaccg tttggtctgt
     9841 tgatgctggt ggtgattgtg ctggcgacgt ttgtctctgg caaacgcgtg cagcttccgg
     9901 cgctggtagc tcatgctggc gtgctgttat ttgccgcggg gatcgtggtt tccagtgtca
     9961 gtcgtcagga gatcagcctg aatttacagc cgggtcagca ggtgacgctg gcaggataca
    10021 ccttccgttt tgagcgactc gatctgcaag ccagaggcaa ttacaccagc gaaaaagcga
    10081 tagtggcgct gtttgaccat cagcaacgca ttggtgaatt aacgccggag cggcgttttt
    10141 atgaagcacg ccgtcagcaa atgatggaac cgtcaattcg ctggaacggc atccatgact
    10201 ggtatgcggt catgggtgaa aaaaccagag cggatcgtta cgcttttcgt ttgtatgtac
    10261 aaagcggtgt gcgctggatc tgggggggag gattgttgat gattgcgggc gcattgttaa
    10321 gcggatggcg ggggaagaag cgcgatgaat aaagggcttc tcacgctact gctgttattt
    10381 acctgttttg cgcgcgctca ggttgtagac acctggcaat tcgccaatcc gcaacaacag
    10441 caacaggcgt taaatattgc cagccagtta cgttgtccgc agtgccagaa tcaaaactta
    10501 ctcgaatcca atgcgccggt ggcggtcagt atgcgccatc aggtttatag catggtgtcg
    10561 gaggggaaga acgaagtcga aatcattggc tggatgaccg aacgctacgg agattttgtt
    10621 cgctataacc caccgttaac gggtcagacg ctggtgttgt gggcgctgcc agtggtgttg
    10681 ttattgctga tggcactgat cctctggcga gtgagggcta agcgatgaaa cagcctcaaa
    10741 taccggtgaa aatgctgaca acactcacga tattgatggt attcctctgt gtcggcagtt
    10801 atctgttaag cccaaaatgg caggcggtac gtgcggagta tcagcgtcag cgcgatccgc
    10861 tacatcagtt tgccagccag caaaccccgg aagcgcagct tcaggcattg caggataaaa
    10921 tccgcgctaa tccacaaaac agcgaacaat gggcgttact gggcgagtac tatctgtggc
    10981 aaaacgatta cagcaattcg ctgctggcgt atcgtcaggc gttgcaacta cgtggtgaga
    11041 acgctgaact gtatgcggcg ctggcgacgg tgctttatta ccaggccagc cagcatatga
    11101 ccgcccagac tcgcgcaatg atcgacaaag ccctcgcgct ggactctaac gaaatcaccg
    11161 ctctgatgct gctggcttcc gatgcgttta tgcaggcgaa ttacgcgcaa gccatcgaac
    11221 tatggcaaaa agtgatggat ctcaactcac cccggatcaa ccggacacag ctggtcgagt
    11281 cgattaatat ggcaaaactg ttgcagcgga gatcggatta atcatctggt gatgaagatt
    11341 atgtcattga gctgcataaa aaataatcga atgaacatat gccaaaaata atcaccaatc
    11401 agtattattg cagattaaac aaataaaaat ctttccataa caaatggtta ttcattaatc
    11461 ctgccataaa ctcgcgttat tatgcattaa tgcagcgaaa agctctgttg ttaaagggtt
    11521 gcgcaacata ccgcgcaaat gatactgatc ataagcgtta aaaaaatcta caaaccaacg
    11581 caacacaatt catgccctgg cagtatgtca cgttctcgcg tttctgaacg gggaacggcg
    11641 ctccattgag gaagttattc tgatgaaaaa tataaaattc agcctggcct ggcagattct
    11701 gtttgctatg gtgctgggca ttctcctggg aagctacctg cactaccata gcgacagccg
    11761 cgactggctg gtcgtcaatt tgctctctcc ggcgggtgat atcttcatcc atctgatcaa
    11821 aatgattgtt gtgccgattg tgatctccac gctggtggtg ggtatcgcgg gtgttggtga
    11881 tgccaaacag ctcgggcgta ttggcgcgaa aaccattatt tacttcgagg tgatcaccac
    11941 cgtcgccatc attttgggga tcactctggc gaacgtcttc cagcccggtg ccggggtgga
    12001 tatgtcgcag ctggcgaccg tcgatatctc gaaatatcag agcactacgg aagcggtaca
    12061 aagcagttcc cacggcatta tgggcacgat tttgtcgctg gtgccgacga acattgtggc
    12121 gtcgatggcg aaaggcgaaa tgctgccgat catctttttc tcggtgctgt ttggtctggg
    12181 gctttcttcc ctgcccgcga cgcatcgtga accgctggtg accgtgttcc gctccatctc
    12241 tgaaaccatg tttaaagtga ctcacatggt gatgcgttat gcaccggtgg gtgtgtttgc
    12301 gctgattgcg gtgacggtgg ctaactttgg tttctcgtct ctgtggccac tggcgaaact
    12361 ggtgctgctg gtgcatttcg ccattctgtt cttcgcgctg gtagtgctgg gaattgtggc
    12421 acgtctgtgc gggttaagcg tctggatcct gattcgtatt ctgaaagatg agctgattct
    12481 ggcgtactcc actgccagct ctgaaagcgt actgccgcga attattgaga agatggaagc
    12541 ctacggagca ccggcgtcga tcaccagctt cgtggtgccg accggttact cttttaacct
    12601 tgatggttcg acgctgtatc aaagtattgc cgctattttc atcgctcagc tgtatggcat
    12661 tgacctgtcc atctggcagg aaatcattct ggtgctgacg ctgatggtga cctcgaaagg
    12721 gattgctggc gtggctggcg tgtcgtttgt ggtgttgctg gcaacgctgg gtagcgtagg
    12781 tattccgctg gaaggtctgg cgtttattgc tggtgttgac cgtatcctcg acatggcgcg
    12841 tactgcgctg aacgtggtgg gtaatgcgct ggcggtgctg gtgattgcca agtgggaaca
    12901 caaatttgac cgcaaaaagg cgctggctta tgagcgtgaa gtgctgggca aatttgataa
    12961 aactgcggat caataattga agattgccgg ggatatcccc cggcaatgtg tggatgcctg
    13021 atgcgacgct tgccgcgtct tatcaggcct accttaaccg ttggttaatt tttcaaactc
    13081 ttcacacccg gtatcaaacc cttccataca gctcaggttc aaccagtgca gcgccttttg
    13141 tttgttcttc tcgataaaac cttcctcacc gtttaagaac atcattcccg cccagtactc
    13201 ggaataaccg gttcgggaaa ttgcagagct gcgtttgaaa taccaggttg ccttgtcatc
    13261 gtcggcctta atgcccacgc cgttggcgta aatcaaacca agcagcattt gggcatcgac
    13321 ggcggagtca ttctccagat cttccgaggc gttttctaac agcgaaatcg cctttggata
    13381 atccggttta cccgcttgag tatttaccag aatatgcgcc agcgttactt caccttcttt
    13441 actgcccgct tgcgccgctt tctctgcaag aacttttgct tgtggatagt ccagactgac
    13501 cggattggtg attttgatct gcgccaacag cgcgcaggca tcagcatcac cgctgtcggc
    13561 ggctttctgt gcccaatatt cggctttgct caaatcgccg gagctaaacc agctgtcggc
    13621 aagaaaatat tgtgcacgtc ggtccccggc ctctgctgcc tttaaatact ggctgccagg
    13681 ctcggagtcg ttcgcgtggg caaagaatgt caaaaacaac atcaatgcga taattttttt
    13741 cattttgata atacgattgg gtagggagta accagtataa agagttcgaa tggataaaaa
    13801 aacagcctcc gaaaggaggc tgtagaaagg actgtattac gccagtgccg cttcgcgcag
    13861 gcgagttttc aacttgttgt actcgtcaat cacgtactgc tcggcggcgc gctgatcgga
    13921 gatcggctcg acgcgcacgg cgcagtattt gtattccggc gttttcgtaa tcgggcttaa
    13981 gctttcggta accagctcgt tacaggcacc aatccaccac tggtaggtca tgtaaatcgc
    14041 ccctttgttc ggacgatcgc tgacctgcgc acgggtgata attttgcctt tacgcgagtg
    14101 cactcaaacc aatgcctcat cttcaatacc cagacgtttg gcgtcttcgg tattgatttg
    14161 tgcgtagcca ggttcatcag ccagcgccgc cagtgccgca cagttaccgg tcatcgaacg
    14221 gcaagagtag tgaccaactt cacgcaccgt tgacagtacc atcgggtact cgtcggtgag
    14281 tttgtcgatt ggcgctaccc agtcgcaggt gaagaactgc gccagaccgt tcggggtatc
    14341 aaacttctct ttaaacagat aagaagtccc ctgatcggca tctgaagtat cgcggcaagg
    14401 ccactgaatg aagcccagtt cgcccatttt ctcgtaagtc gcaccgtaga aatccgggca
    14461 cagatgacgc aactcatccc agatctcctg ggtgttgttg tagtgcatcg gataacccat
    14521 acgggtggcg atttcactga tgatttgcca gtccgttttc agatcccatt tcggttcaac
    14581 tgctttgaag aaacgctgga agccacggtc agccgcagta aacacgcctt catgctcgcc
    14641 ccacgacgtt gacggcaaaa tgacatccgc cgccgacgcg gttttggtta taaagatgtc
    14701 ctgaacgatg accagttcca gatcttcaaa ggctttacgt actgccgaca gctccgcatc
    14761 agtttgcagc ggatcttcgc ccataatgta cgcggcacgc acttcgccat gcgctgcgcg
    14821 gtgcggcagc tcgctgatgc gatagccggt atgagcaggc aggctttcca cgccccaggc
    14881 tttggcgaat ttttcgcggt tagccggatc tttcacgtac tggtatcccg gatacgtatc
    14941 cggcagcgcg cccatatcgc aggcaccctg cacgttgttc tgaccacgaa ccgggtttac
    15001 acccgcatgc ggcttaccga ggttactggt cagcatcgcg aggctggtca gagaacgcac
    15061 ggtttccacg ccctggtaga actgggttac acccatgccc cacaggatgg cggcgctttt
    15121 cgcctgggca tacatccgtg ccgcctgacg aatctcactg gcgctgacac cggtgatatc
    15181 ttcaaccgac tccggcgtgt agccttcaac gattttacga tactcttcaa agccttctgt
    15241 acgggaagcg acgaacgctt tgtcgtacag attttcttca ataatgacat ggcccatcgc
    15301 attcaacagc gcgatgttcg agccgttttt cagtgcaatg tgcatgtcag caatgcgcgc
    15361 ggtttcaatt ttgcgcggat cgcagacgat aattttcgcc ccgttacgtt tagcgttaat
    15421 tacgtgattc gccacgattg ggtgggaatc cgccgggttg tacccgaaaa cgaacactaa
    15481 atcggtatta tcaatttcgt taatagcatt gctcattgcg ccattaccga ccgattggtg
    15541 cagacctgca accgatgggc cgtgtcagac acgagcgcag cagtcaacgt tattggtacc
    15601 aataacggcg cgcgcaaatt tttgcattac atagttggtt tcgttacccg taccacgcga
    15661 ggagccggtc gtctggatgg catccggacc gtacttctct ttgatggcgc tcaggcgctc
    15721 ggcaacgtaa ttcagtgcct catctcagga aacaggttcg agtttgccgc cacgctggcg
    15781 acggatcatg ggggttttca ggcgcggggt caggatctgg gtatcgttaa tgaaatccca
    15841 gccataataa cccttcagac acagggtacc ctggttggtt ttcccctgcg ctgcctccgc
    15901 ccggacgatt ttgccgttat cgacgaccag gttgattttg caacctgatg cgcaataggg
    15961 gcaaaccgtg acgacttttt tcatcggtct cgctccagtt aatcaaatca cgcatacgcg
    16021 ctctcgacta cagtatgcat cttttatgcc acattttatt tggggtcatt ccctgatatt
    16081 acgggcacta tttattcaaa actctgacga aaaacaggct gtcgtcagtt ttgacgtgac
    16141 gaaacgaaat accgcgtgac agccatcacg cggcagacat tttatttttt ctcgacgacg
    16201 ggagccgcct gatacccgcc gcccagcgat ttcatcaact gaatgctttg gatcacccgg
    16261 cggctgtcca gcatcagtaa tgacatctct tcggcaagta ctggcaaccg ggcttcagtg
    16321 gcctgtaagc ggctggttaa gccgcgctga taggcggcct cggcagcgcg ctgggtgaag
    16381 cgcgttgctt ccacgtgttc agcctgcatt tctcgctcgt cgttgagcgt ttgcagacgc
    16441 gtgccgttga cggcaacgtc acgcaccgcg ttcagtactg actggttgta acgttcaatc
    16501 atcatgttgc tggcggcgcg cgtgccttcg agattggcat tcaaccgtcc accgtcaaac
    16561 agcggcaatt tcagacccgg gatgaagttg aactggcgac tggttttttt gaataaggta
    16621 tccaggtgga tggcatcaag gccgaaaaac gctttgatat caaagctcgg atagaacaac
    16681 gcccgcgcgg aatccacctg atctaatgac gcctgaacat accagcgcat ggcttgcaga
    16741 tccgggcgtc tggcgagcaa ctcataagag agtgtcgccg gaatgccggt ctggactcgt
    16801 ggtaatgcca caggtttgat ctccggcata tcgctggctc ccgcgccaat caatgcgcgc
    16861 agagattctc tcgtttcggt gatttgccct ttgacggcag caatttgttt atcgaccgcc
    16921 agaatctgtg cccgcgcgcc gtggaaaggc acttgtgctt ccagaccgtg cgccacttta
    16981 ctttggtgcg ctttcaccgc gtaatcaatc acatcgcgag tttgttctaa cagatcgagc
    17041 atctgatagc tggcctgcat actgtaataa agctgcgcta cgcccgtggt cagcgatagc
    17101 tctactgctg cggtttctgc cagcgcggca ttatgcgcgc caatggcggc ggcaaccgct
    17161 gagcgatgca caccccacaa atcaagatcc agtccggcaa acaaacctac tgtggcttcc
    17221 gtatagtacg gcccgtccat ccccagtgcg ggcgcatcca tcgcataagg gcttaaaaag
    17281 ccgttcgccg agacacgttg gcggttaagc atccctaacg ctgccacctg caactgtgaa
    17341 ccggcatcta acaaatcggc ctgcgattgc gctttttctt cccgcagttt cgcttcggcg
    17401 agggtgtgtg aaccacttag cgtccgttgg atcagggcat ccaactgcgg gtcattgagt
    17461 tgtttccacc actgcgcctg cggccagccg gagctggcaa gatgaatatc atcggccagt
    17521 ttgatttgtt ccggtttgag ctgttgatga ggcgcagaat ccttacgtac cagggcacag
    17581 ccggaaatca gcgtcgtgct gccgagaatg ctgcacaaca gcagacgtga aagttgacga
    17641 ttgatcattg cgtggctcct tgtgcctgtt ccgtggcggg cgttaatgcc ggggctgtcc
    17701 agtctggcaa gcgggcgaaa agctggcaga cgtgttgctc ctgttttaat aaggtgggca
    17761 ggatggcctg cgaggtgggt gtctcttcaa gcgttatttg aggagaacgg ctgagtgcgg
    17821 ttgccagacc ggcagcgtat ttctccaggg cgtcggcaaa ctgacctgct ctgtccggct
    17881 gtagtgcgtt atccagcgcc tgcgccgaat tccaggtggc atcccaggcg tgaaggatat
    17941 cgcggccctg acgaataacc gtttgcgaac gttcaatcag taaggcgcgt tcttcgctgt
    18001 ccagttgacg ctccagcgcc acgcgttggc acatctcttc acaggcatta aacgccgcat
    18061 gcagaccgat acgaatttgc agataagtgc gcagagccgt gacttcctgc tggcgtggga
    18121 tccgcattac tttacttaac atacccagcg cgccagccag tttttgcggc agcgtgcgcg
    18181 cttcactttc aggccagaca aaggtgtaaa tcatcgcgga caccacggta ccaatgatga
    18241 tacccagggc gcgatcgcga atttccacca ggtcgtacac tgggccaaaa acgttttcga
    18301 gcgtggcgag cgcgaaggtg accaccatct gtgtgccgat ataagaagag cgttcagagc
    18361 tggtggcaat ccatgcgccc aacaggaaaa tcggtgccag cacaaacagc aattcgacaa
    18421 tattgtccag ccagggcatg accagtagcg tgaataacag cgccagaatc gcgccgcaaa
    18481 aggccccgcc aaaacgcagc accatcttct ggtacgacga accgacattt ggattagcga
    18541 cgatcacgca ggtcagcata caggtgtgaa tgccttccca gtccacgccg ctgtaaaagg
    18601 tgtaacagat caaacaggcg agcaatgttt ttaccgcgta gcgtatatag tctggattgg
    18661 taaaagcatc ggcgaccatt gatggcggtt tggcggcggg cgttggcggc gtattcgggt
    18721 tcatctggcc cagttgtaac aacgtctggc agatattctc caggttacat tcccgtgccg
    18781 ccaccgcttc actttcactg agccgccagt cgctttgcca gcactgacct tcagcaacgg
    18841 catgctgcag cttgttgatt tctgaggcta atttttgccg aaattcaata attgcctgag
    18901 aatcagcaaa agaggtggga tcgtagcgat tcagcgtcga gtaaatgtag gttaccgttg
    18961 ccacgcagct ttgccaccat gcgctttgag ttcgccagtt ggcatcgtcc gcgagacaaa
    19021 agacattcag tttttgcagc gccagcgcct ctctttcaat ccgcgtttcg ggtagcggtg
    19081 cgaggctgtc cgtcaggtgg ctaatggcat catcaagccg atcattaagc gcttgatgca
    19141 tttgcgtaat ggcacgattg ggaaaccaca gcacgccgat taacgtcatc agcaaggttg
    19201 gatagaggcc aacaacgata caccacagcg ttaagcgcac gactacttcc ggatagtcga
    19261 gcatggcggg gaaggtttgc ccgtaaatgg cgacagcgaa aaagaccagt cccagccgat
    19321 gggtgcgcat caaaaacatg cagcccatca ggatcggccc ggcgatgatc aatcggatca
    19381 acggttcgcc gtatgaccat ttgtagatca aaaacaggct gccgatctcc agcaccgtgg
    19441 caaccacaaa caagatcgcc acaaatttgg tgtaaaacgc gttcgactga ataccgtaaa
    19501 acagcactgc cagcgataac gccacaaaag ggatctcaaa agtcatcgag atcagaatca
    19561 ccagcaggca gcctacccag agttgcacga tctgcggcac gcggccgggt cgccgctcgc
    19621 ttaactcttc atgaaagaac gccagcagcc tgaccaccgg taatggcagg gagttgagcg
    19681 cgctcattat tgcggctcaa gattagcgac tgccgaagcg ccgatgcgga acatttccgg
    19741 gtcaggttta tcgaccatga ttttgaccgg aaaacgctgg gcaacgcgga cccagttaat
    19801 agaacgagac acttttggca ggccgcccag caccaggccg ccgtcatccg gtagcacgcc
    19861 gtagccaatc gaatccactt taccctcgaa ggttttgccg ctgtcactca tcaggcgaat
    19921 cgttgcgggt gtacctgagc gaatattttt cagatcggtt tcgcggaagt tggcgatcac
    19981 gtaccagtga cgagtgtcga tgagggtaaa aatagggcgc atggcagaag caaattgccc
    20041 gacggaggtt ttgagggaaa tgatccggcc atcaaatggc gcgcgaacgg tcgtcatttc
    20101 cagatgtagt ttggtcaggg caatatccgc ttcgaccgcc gcacgttgcg ccaccagcgc
    20161 atcgacgccg ctgacggcgc tggcggctga ctgcgcctgt aacaatacgg cattaagatc
    20221 cgcttctgcg gcgcgctgcg ccgttcttgc acggtcaacg tcttctgctg agacaaaacc
    20281 ctctttcagt aatggctcgg tgcggcgtaa tgtatctgtg gcctgtttcg cggcggcacg
    20341 ggctttttct accgtggcat taaccgagtc ggcaccaaac tgttgggcgt caacgctacg
    20401 ctgggtgagc ataatttgct tatccagcgc cgcgagggag gcttcggctt tcgccaggtt
    20461 ggcttcgtac gggcgcgggt cgatgcggaa cagcaaatcg ccctgtttca ccgcctggtt
    20521 gtcggtgacc gccagttcta caatgcgacc gctgacttcc ggcaccacat caatggtatc
    20581 tgctgacgcg taagcgtcat tagttgatgg cgcactgtct acgcgccaga taacgaaaac
    20641 aagggcaacc agcgccaacg caaccactaa cagagcaggg aatttactgc gaggtgcttt
    20701 tttcggcgta ctttccatga gcgcgtctca aatagattag aaaaatgcca gccagaacag
    20761 catggcgtag agagcaaaaa gggcggtata aataattccg acaaatgcca gattgatatt
    20821 tgcccg
//
LOCUS       DAZSNM010000058        20708 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZSNM010000058 DAZSNM010000000
VERSION     DAZSNM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20708
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.058"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACHW42_001760"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACHW42_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR8943611.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACHW42_001761"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACHW42_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR8943612.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACHW42_001762"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACHW42_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR8943613.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACHW42_001763"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACHW42_001763"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR8943614.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHGYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="ACHW42_001764"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="ACHW42_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR8943615.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="ACHW42_001765"
     CDS             complement(8217..9110)
                     /locus_tag="ACHW42_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR8943616.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACHW42_001766"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACHW42_001766"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR8943617.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACHW42_001767"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACHW42_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR8943618.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACHW42_001768"
     CDS             complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACHW42_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR8943619.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACHW42_001769"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACHW42_001769"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR8943620.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACHW42_001770"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACHW42_001770"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR8943621.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="ACHW42_001771"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="ACHW42_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACHW42_001772"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACHW42_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR8943622.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACHW42_001773"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACHW42_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR8943623.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACHW42_001774"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACHW42_001774"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR8943624.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="ACHW42_001775"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="ACHW42_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR8943625.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4981 a         5727 c         5029 g         4971 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataacca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatcctca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
    20701 ccgacaat
//
LOCUS       DAZSNM010000059        20520 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZSNM010000059 DAZSNM010000000
VERSION     DAZSNM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20520
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.059"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="ACHW42_001776"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="ACHW42_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR8943626.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="ACHW42_001777"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="ACHW42_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HFR8943627.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="ACHW42_001778"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="ACHW42_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR8943628.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACHW42_001779"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACHW42_001779"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR8943629.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACHW42_001780"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACHW42_001780"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR8943630.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="ACHW42_001781"
     CDS             4449..4814
                     /locus_tag="ACHW42_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR8943631.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="ACHW42_001782"
     CDS             4964..5482
                     /locus_tag="ACHW42_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR8943632.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNVFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="ACHW42_001783"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="ACHW42_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="ACHW42_001784"
     CDS             6713..7195
                     /locus_tag="ACHW42_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR8943633.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="ACHW42_001785"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="ACHW42_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR8943634.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8427)
                     /gene="trmD"
                     /locus_tag="ACHW42_001786"
                     /pseudo
     CDS             complement(7661..8427)
                     /gene="trmD"
                     /locus_tag="ACHW42_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
     gene            complement(8458..9006)
                     /gene="rimM"
                     /locus_tag="ACHW42_001787"
     CDS             complement(8458..9006)
                     /gene="rimM"
                     /locus_tag="ACHW42_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR8943635.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9025..9273)
                     /gene="rpsP"
                     /locus_tag="ACHW42_001788"
     CDS             complement(9025..9273)
                     /gene="rpsP"
                     /locus_tag="ACHW42_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR8943636.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9410..10771)
                     /gene="ffh"
                     /locus_tag="ACHW42_001789"
     CDS             complement(9410..10771)
                     /gene="ffh"
                     /locus_tag="ACHW42_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR8943637.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10938..11729
                     /locus_tag="ACHW42_001790"
     CDS             10938..11729
                     /locus_tag="ACHW42_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR8943638.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11750..13036
                     /locus_tag="ACHW42_001791"
     CDS             11750..13036
                     /locus_tag="ACHW42_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR8943639.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13091..13684)
                     /gene="grpE"
                     /locus_tag="ACHW42_001792"
     CDS             complement(13091..13684)
                     /gene="grpE"
                     /locus_tag="ACHW42_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR8943640.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13807..14685
                     /gene="nadK"
                     /locus_tag="ACHW42_001793"
     CDS             13807..14685
                     /gene="nadK"
                     /locus_tag="ACHW42_001793"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR8943641.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14771..16432
                     /gene="recN"
                     /locus_tag="ACHW42_001794"
     CDS             14771..16432
                     /gene="recN"
                     /locus_tag="ACHW42_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR8943642.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16581..16922
                     /gene="bamE"
                     /locus_tag="ACHW42_001795"
     CDS             16581..16922
                     /gene="bamE"
                     /locus_tag="ACHW42_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR8943643.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16984..17274)
                     /locus_tag="ACHW42_001796"
     CDS             complement(16984..17274)
                     /locus_tag="ACHW42_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR8943644.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17264..17701)
                     /gene="ratA"
                     /locus_tag="ACHW42_001797"
     CDS             complement(17264..17701)
                     /gene="ratA"
                     /locus_tag="ACHW42_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR8943645.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17872..18354
                     /gene="smpB"
                     /locus_tag="ACHW42_001798"
     CDS             17872..18354
                     /gene="smpB"
                     /locus_tag="ACHW42_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR8943646.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18569..18931
                     /gene="ssrA"
                     /locus_tag="ACHW42_001799"
     tmRNA           18569..18931
                     /gene="ssrA"
                     /locus_tag="ACHW42_001799"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19136..20377
                     /locus_tag="ACHW42_001800"
     CDS             19136..20377
                     /locus_tag="ACHW42_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR8943647.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5300 a         5357 c         4814 g         5049 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatttgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acatcccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atgtttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attgcaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg atgaccattc
     8041 ttcgtcaatt tcggtttgga tcacgcgctc atctatacct tcgtagcgac cgcacaccag
     8101 aatcaatttt tgattcgttg ccagttcgct gacgcccgct tgatcaagct tgcgtccctg
     8161 tggtgacaga taaatcacct ttgcgccttc acccgccgcg gcttttgctg catgaatggc
     8221 gtcccgcaag ggttgcacca tcattaacat ccccggtccg ccgccgtaag gacgatcgtc
     8281 cacggtacgg tgccggtcat gcgtgaagtc gcgaggactc caactctgga tgctcagcag
     8341 gccattttta actgcccggc cagttacccc gtaatcggta attgcgcgga acatttcagg
     8401 aaacaggcta attatgccaa tccacatagc gccgcctttt accgtttatc cggtggttta
     8461 aaaaccagga tcccaatcta cttcgattga acgagtagtg agatcgactt tcttgataac
     8521 ctgcccatcg aggaacggta cgagacgttc cttgatacca aacgcatctt tcaggtttgc
     8581 cttaatgacg agaacgtcat tagatccggt ttccatcata tcaacgactt taccgagatc
     8641 gtagccttca gtggttacta cctggcagcc catcaggtct ttccagtagt agtccccctc
     8701 ttcaagctga ggcagctgcg atgaatccac gacaatttca caattcgtca gcaggttcgc
     8761 cgcatcacga tcgtcaacgc ctttcagctt gatgatcatg tcctgattgt ggtgcttcca
     8821 gctttccagc tggacttgct gccactgacc cgccttctgg ataaaccagg gctgatagtc
     8881 aaaaatgctt tcggcgtctt cggtggaaga aaacactctg agccacccac gaataccgta
     8941 agacgaaccc atttttccca aaacgatggg atcaacaggt gcttgcgcgg tgagttgttt
     9001 gctcatcatg accaccgtga cagattaagc tgctttgttt acttctttga tcagcgcagc
     9061 aacgcgatca gaaatagttg cgccctggcc aacccagtga gcgatgcgat ccagatccag
     9121 gcgagtgcct tcttcttttt cgctagcgat tgggttgaag aaaccaacgc gctcgatgaa
     9181 gcgaccgttg cgtgcattac ggctgtcagc gacaacaacc tggtagaacg gacgcttttt
     9241 agcgccgtga cgtgctaaac gaatagttac cataacatcc tcttgtgtga ataaaacaac
     9301 cggaccccat cgaggaacgg agtccggtgt catattaaaa gcccgaaaat tttactgatt
     9361 tctggagaaa atgcaatcag cagttaataa ctctgtcgca taaggtcatt tagcgaccag
     9421 gaaatcccgg cggcatcatt cccttcatgc ttctcatcat cttcgccatt ccgcctttct
     9481 tcattttctt catcatgcgc tgcatgtcgt cgaactgctt cagaaggcgg ttaacgtcct
     9541 gtacctgcat cccgcaaccg gcggcgatac gacgcttacg cgaacctttg atgatttctg
     9601 gcttagcgcg ctctttcatc gtcattgagt tgatgatggc ttccatacgc accagcactt
     9661 tatcgtccat ctgcgacttg acgttatccg ggatctgccc catgcccggc agtttgccca
     9721 tcaagctggc catgccgccc atatttttca tctggcgcag ttgctcaaga aagtcgttga
     9781 gatcgaagcc gtcgcctttt tttagcttgc tggctaattt ctctgcctgc gcgcggtcaa
     9841 ctttgctttc gatatcttcg atcagcgaca gtacgtcgcc catgccgaga atacgcgacg
     9901 cgatgcggtc cggatggaac ggctccagcg cctcagtttt ctcgccaaca ccgaggaact
     9961 tgatcggttt gccagtgatg tgacgaatag agagcgccgc accgccgcgg gcatcgccgt
    10021 ccactttggt caacactacg ccggtaagcg gtaacgcttc attgaatgct tttgccgtat
    10081 tggccgcatc ctgaccggtc atggcgtcaa ccacaaacag ggtttcaacc gggttaatcg
    10141 acgcatggac ttgtttgatc tcgtccatca tcgcttcgtc aacgtgcaga cgaccagcgg
    10201 tatccaccag cagcacgtcg tagaatttca gtttggcttc tttcagcgcc gcgttaacaa
    10261 tatcaaccgg cttctgacca acatcagaag ggaagaaatc aacgcccacc tgctctgcca
    10321 gcgtctcaag ctgtttgatt gccgccgggc gatagacgtc agcagaaacc accagcactt
    10381 tcttcttgtg cttctcgcgc aggaaattac cgagtttacc aacgctggtt gttttaccgg
    10441 caccttgcag gcctgccatc agcacgaccg caggcggttg cgcagccaga ttcagggtct
    10501 ggttctcttc gcccatcgcc gcaaccagtt cgttacggac gattttgacg aactcctgcc
    10561 ccggcgtcag gctcttatta acttcatgac caaccgcttt ctcttttacg cgattgataa
    10621 actcacgcac taccggcaga gcgacgtccg cctccagcag cgccatgcgc acttcgcgca
    10681 gcgtatcttt tacgttgtct tcagtgaggc gtccacggcc actgatattg cgcagcgtgc
    10741 gcgacaaacg atcggttaaa ttatcaaaca ttgtctctcg cctggggtgg aaacggttgg
    10801 ccgcaatcgc gacacatcat cagtattttg ccgcagtata acatgaaggc gtctttgttg
    10861 ttatgcaacg gttggagcag cgttcacctg acgctatact gcttctcttt cttattgctc
    10921 aaactgtcga cattactatg cccgtttttg ctctgctcgc gcttgtcgcc tactccgtca
    10981 gtcttgcgct gattgttccc ggtctgctgc aaaaaaacgg cggctggcgg cgcatggcta
    11041 ttatttctgc ggtcattgcg ctggtctgcc acgcaatcgc tctggaagcc cgcatcctgc
    11101 ccgacggtga tagcggacaa aacctcagcc tgctgaacgt tggttcattg gtcagtttgg
    11161 tgatctgtac ggtaatgacc attgtggctt ctcgcaatcg tggctggttg ctgctaccca
    11221 ttgtctatgc ctttgcgctt atcaacctgg cgctggcgac cttcatgccc aatgaataca
    11281 tcacccatct ggaagcaacg cctgggatgc tgatgcacat tggcttatcg ctcttttcct
    11341 atgccacgct aattatcgcc gccctgtacg cgctgcaact ggcgtggatt gattaccaac
    11401 tgaagaacaa gaagctggca tttaaccaag aaatgccgcc attgatgagt atcgagcgta
    11461 aaatgttcca catcacgcag attggcgtgg tgctgctaac gctcacgctt tgcactggcc
    11521 tgttctacat gcacaaccta tttagcatgg aaaatatcga caaggctgtg ctctctatcg
    11581 tggcgtggtt tgtctatatt gtgctgctgt ggggacatta ccatgaagga tggcgtggac
    11641 gccgcgtcgt ctggtttaac gttgcgggcg cggtaattct gacactggcc tacttcggca
    11701 gccgaattgt ccagcagtta atcagctaaa cccagaaaag gagtttcccc tggaacacat
    11761 ttctactact acgttgatca ttattctgat catcatggtg gtcatttcag cctatttttc
    11821 cgggtccgaa accggaatga tgaccctcaa ccgctatcgt cttcgacata tggcgaaaca
    11881 gggtaatcgc tcggccaaac gcgtcgaaaa attgctgcgt aagccagacc gcctgataag
    11941 cctggtgtta atcggcaata acctggtcaa tattcttgcc tccgcgctcg gcactattgt
    12001 cgggatgcgt ttgtacggcg atgcgggcgt ggcaattgcg actggtgtgc tgacttttgt
    12061 cgtactggtt tttgctgagg tattgccgaa aaccattgcc gcgctgtacc cggaaaaagt
    12121 cgcttatccg agtagttttc tgctggctcc gctgcaaatt ttgatgatgc cgctggtctg
    12181 gttgctgaat gctatcaccc gtatgctgat gcgcatgatg ggtatcaaaa ccgatatcgt
    12241 ggttagcggc tctttgagca aagaagagtt gcgcactatc gtgcatgaat cgcgctcaca
    12301 aatttcccgt cgcaatcagg atatgctgct gtcggtgctc gatctggaaa aaatgaccgt
    12361 tgatgacatc atggtaccgc gcagtgaaat tatcggtatt gatatcaacg atgactggaa
    12421 atctattctg cgccaactct cccactcacc tcacgggcgc atcgtgcttt accgtgattc
    12481 gctggacgac gccatcagta tgctgcgagt acgtgaagcc tggcggttga tgtcggagaa
    12541 aaaagagttc accaaagaaa ccatgctgcg cgccgcggac gagatctatt ttgtgcctga
    12601 aggtacgccg ctcagcacgc agttggtaaa gtttcagcgc aacaaaaaga aagtcggcct
    12661 ggtcgtcaac gagtatggag acattcaggg gctggtgacg gttgaagata ttctggaaga
    12721 gattgttggc gatttcacta cgtcgatgtc gccaacactt gccgaagagg tcacgccgca
    12781 aaacgacggt tcggtgatta tcgatggcac cgccaacgtg cgggaaatca acaaagcctt
    12841 taactggcat ctaccggaag atgatgcccg cacggttaat ggcgtcattc ttgaggcact
    12901 ggaagagatc cctgtcgcag gcacccgcgt gcgtattggc aagtacgata tcgatattct
    12961 cgacgtacag gacaatatga ttaagcaggt aaaagttttt cctgtgaaac cgctgcgcga
    13021 gagtgtggcg gagtaacgaa aacggccctg cattcgcatg cagggccgtg aattattacg
    13081 aaaacagaaa ttacgctttt gctttcgcta cagttaccat cgccgcacga atcgtacgac
    13141 cattcagcgt ataacccttc tgcataatgc ccagtacgtt acctggcgca acgtcatcag
    13201 attccaccat tgcgatagcc tgatgcacat tcgggtccag cggcacgtta gtttcggcga
    13261 tcacttcaac accaaactta cgcacaacat ccagcatcga cttcagcgtc agctcaatgc
    13321 cttcaaccat cgcagacata tccgggttag ctttatcagc cacttccagc gcacgatcca
    13381 ggctatcaat caccggcagc aattcgttga tgaatttctc cagcgcgaat ttgtgggctt
    13441 tttcaatatc cagttcagta cgacgacgca ggttttccat ttcggctttt acgcgcaaaa
    13501 taccgtcacg ttcacgggtc tgggcttcag ccagctgagc ttcgagattc gcaatttttt
    13561 catcgcgcgg atccacctgc tcagcagaag cttctggctc aactgcctca atctcttcgt
    13621 gctgatccat gataatttct tccggggctt gcccctcagg cgttttctgt tctttactac
    13681 tcatgaattt ctccgcgttt ttttcgcatt catctcgcta acttcgctta ttatggggat
    13741 cagtttcagg gtttcaaggg aagcactcac attgtcatca atcttcgcaa caaggacctc
    13801 ggaaaaatga ataatcattt caagtgtatt ggcattgtgg gacacccacg gcaccctact
    13861 gcactgacaa cacatgaaat gctctaccgc tggctgtgca caaaaggtta cgaggtcatc
    13921 gttgagcaac aaatcgctca cgaactgcaa ctgaagaatg tgaaaactgg cacgctcgcg
    13981 gagattgggc aactggctga tctcgcggta gtcgttggtg gcgacggtaa tatgctgggc
    14041 gcggcgcgca cgctcgcccg ttacgatatt aaagttattg gaatcaaccg tggcaacctg
    14101 ggtttcctga ctgaccttga ccccgataac gcccagcaac aattagccga tgtgctggaa
    14161 ggtcactaca tcagcgagaa acgttttttg ctggaagcgc aagtctgtca gcaagattgc
    14221 caaaaacgca tcagcaccgc gattaacgaa gtggtacttc atcctggcaa agtggcgcat
    14281 atgatagagt tcgaagtgta tatcgacgag atctttgcgt tttctcagcg atctgatggg
    14341 ctaattattt cgacgccaac aggctccacc gcctattccc tctctgcggg cggtcctatt
    14401 ctgaccccct ctctggatgc gattaccctg gtgcccatgt tcccgcatac gttgtcagca
    14461 cgaccactgg tcataaacag cagcagcacg atccgtctgc gtttttcgca tcgccgtaac
    14521 gacctggaaa tcagttgcga cagccagata gcactgccga ttcaggaagg tgaagatgtc
    14581 ctgattcgtc gctgtgatta ccatctgaat ctgattcatc cgaaagatta cagttatttc
    14641 aatacattaa gcaccaagct cggctggtca aaaaaattat tctaatttta cgccagcctc
    14701 tttactgtat ataaaaccag tttatactgt acacaataac agtaatggtt tttcatacag
    14761 gaaaacgact atgttggcac aactgaccat cagcaacttt gctatcgttc gtgagcttga
    14821 gattgatttt catagcggca tgaccgtaat aactggcgag accggtgcgg gtaaatctat
    14881 tgcaatagat gccctcggtc tttgtctcgg tggtcgcgct gaagccgaca tggtgcgtac
    14941 cggcgctgct cgcgctgacc tgtgcgcccg tttttctctg aaagatacgc cagcggccct
    15001 gcgctggctg gaagaaaacc agcttgaaga cgggcatgaa tgtttgcttc gtcgcgtgat
    15061 cagcagtgat ggtcgctccc gtggtttcat caacggtaca gctgttcctc tgtcacaact
    15121 gcgcgaactg ggtcagttgc tgattcagat ccatggtcag cacgctcatc aattactcac
    15181 caaacctgag caccaaaaat tcctgcttga tggctatgcc aatgaaacct ctctacttca
    15241 ggaaatgacc gcacgttatc agttgtggca tcaaagctgc cgtgacctcg cgcatcatca
    15301 acagttaagt caggaacgcg ccgcccgtgc ggaactgctg caataccaat taaaagaact
    15361 taacgaattt aatccgcagc ccggagagtt tgagcaaatc gacgaagagt acaaacgtct
    15421 ggcgaacagc ggtcaattgc tgaccaccag ccagaatgca ttggcattaa tggccgacgg
    15481 tgaagacgca aacctgcaaa gtcagcttta cacggctaaa caactggtga gcgaattgat
    15541 tggcatggac agcaaactgt ccggcgtact tgatatgctg gaagaagcta ccatccagat
    15601 tgctgaagcc agcgacgaac tgcgccacta ctgcgatcgt ctggatctcg atcccaaccg
    15661 actgtttgaa cttgaacagc gcatctcaaa acagatttcg ctggcacgta aacatcacgt
    15721 cagccctgag gcattaccac agtattacca gtcgctactg gaagaacagc agcaactgga
    15781 cgatcaggcc gactcacaag aaacgctcgc gctggcggta acgaaacatc atcagcaggc
    15841 actggaaacc gcgcgcgcat tacaccaaca acgccagcaa tatgcagaag aacttgcaca
    15901 gctgatcacc gacagtatgc atgcgctctc aatgccgcat gggcagttta cgattgatgt
    15961 caaatatgac gagcatcacc tgggcgctga cggtgccgat cgtattgagt ttcgggtaac
    16021 caccaaccca ggtcagccaa tgcagcctat tgccaaagtc gcatccggtg gtgaattgtc
    16081 ccgcatcgca ctggcaatcc aggtcatcac ggcgcgtaaa atggaaaccc cggcactgat
    16141 ttttgatgaa gtggatgtag ggattagcgg tccaacagcg gcagttgtcg gcaaactgct
    16201 gcgtcaactc ggcgaatcaa ctcaggtgat gtgtgttacc cacctgccac aagtcgcggg
    16261 atgtggtcat caacactatt ttgtcagcaa agaaaccgat ggtgcgatga cagaaacaca
    16321 tatgcaatct ctggataaaa aagcgcggtt acaagagctg gcgcgcctgc ttggtggcag
    16381 tgaagtcaca cgtaacacac tggcgaatgc gaaagaactg cttgcagcgt aaactttttt
    16441 cctgcttcac ggtcagagta aacagcaaaa cgccgtaaga ccggaaagca aaaggtttta
    16501 aagtgatgaa aggtctatta tcatcggcat attacagatg agccacgtac tgctcgggcc
    16561 cgaaaaggaa tcaaatcact atgcgctgta aaacgctgac tgctgcagca gcagtactat
    16621 tgatgttaac cgcaggctgt tccactctgg agcgagtggt ttaccgtcct gacatcaacc
    16681 aggggaacta tctgaccgct aacgacgtat ccaaaatacg tgttggcatg acgcaacaac
    16741 aagttgcgta cgcattgggt acaccgctga tgtccgatcc atttggtacg aatacctggt
    16801 tctatgtctt ccgccagcaa ccaggtcatg aaggtgtaac gcagcaaacg ctgacgctga
    16861 cctttaacag tagcggtgtg ttgaccaata ttgataacaa acctgcgctg agtggtaact
    16921 aataataaag ttgctctcaa agacgttaaa aaaggtgctc aatgagcacc ttttttctgt
    16981 ctgttattta ttcgctgatt tttctgctcg ttgcctgcga agctctttcg gatcggcaat
    17041 gagagggcga taaatctcca cccgatcgcc atcatgcaca ctatcgctta gttttgccgg
    17101 acggctgtaa atgccgactt tgtttttagt taaatcgata tcggtacgca attccagcaa
    17161 gccactggcg cgaatagctt cttcaaccgt cgcgccctcc tgcagcgtca ctcgctgcag
    17221 gtattgcttc tcaggtagcg cataagccac ctcaacggca attttaccta gcactgtaaa
    17281 cctctttcgc acgaaccgta aaagcctgga ccatattagc cgccagctct ttaaacacgc
    17341 gaccaaaggc gagttcaatc aacttattgg taaactcaaa gtcgagatga aactcgatac
    17401 gacacgcctc ctggctcagc ggcgtaaact tccatccacc aatcaatttc ttgaacggcc
    17461 catccaccag attcataaga atactttggt tactggtcaa ctggttgcgg gtagtaaacg
    17521 ttttgctgat cccagcctta gagacatcta ccgccgcagt catctgccca ggagtggact
    17581 ccagaatccg acttccggta caacctggca aaaactgagg ataagactga acgtcattca
    17641 ctaactgata catttgctcc gcgctgtagg gtaccagtgc ggtccggcta atctgaggca
    17701 taacaatttc catcaacaaa aatccaacaa ataatatcat ttatcctgct gtaaaaaaaa
    17761 cgctatcccg gcgctgggta acatcgggtt catgctaaga tagagccttg tcccccgcag
    17821 gattgatatg gggtattttc gatttcagat taccgatgat tcacgacgct tatgacgaag
    17881 aaaaaagcac ataaacctgg ttcagcgacc atcgcgctta acaagcgcgc ccgtcacgaa
    17941 tactttatcg aagaagagtt cgaagcggga cttgccctgc aaggctggga agttaaatcc
    18001 ctgcgcgcag gaaaagccaa tatcagcgac agctacgtcc ttctgcgtga cggagaggca
    18061 tttctgtttg gcgctaacat cacgccaatg gccgtggcct ccacgcatgt ggtgtgcgat
    18121 cctacccgta cccgcaagtt acttctcaac cagcgcgaac tggactcatt gtacggtcgc
    18181 gtcaatcgag aaggctatac cgtagtggcg ctctccctgt actggaaaaa tgcctggtgc
    18241 aaagtgaaaa tcggcgtcgc caaaggtaaa aaacagcacg ataaacgttc agatatcaaa
    18301 gaacgcgaat ggcaggtgga taaagcacgc atcatgaaaa acgcccaccg ttaaacctgc
    18361 actccaatta ttgaccagtt cctcaccgcg cctccctctc cggcggcgcg aatgaacatc
    18421 ttattggcta tcacatccga cacaaatgtt gccatcccat tgcttaatcg aataaaaatc
    18481 aggctacatg ggtgctaaat ctttaacgat aacgccattg aggctggtca tggcgctcat
    18541 aaatctggta tacttacctt tacacattgg ggctgattct ggattcgacg ggatttgcga
    18601 aacccaaggt gcatgccgag gggcggttgg cctcgtaaaa agccgcaaaa aatagtcgca
    18661 aacgacgaaa actacgcttt agcagcttaa taacctgctt agagccctct ctccctagcc
    18721 tccgctctta ggacggggat caagagaggt caaacccaaa agagatcgcg tggaagccct
    18781 gcctggggtt gaagcgttaa aacttaatca ggctagtttg ttagtggcgt gtccgtccgc
    18841 agctggcaag cgaatgtaaa gactgactaa gcatgtagta ccgaggatgt aggaatttcg
    18901 gacgcgggtt caactcccgc cagctccacc aaaattctcc atcggtgatt accagagtca
    18961 tccgatgaag tcctaagagc ccgcacggcg caagccctgc gggctttttt gtgctccgaa
    19021 tttgtcccgc gaagtctgat gccaactaat taaatccgat ccttttaggc accttgttag
    19081 gcacttcata aagctatatt gtctttgagg tgcctaaaac tatggaaacc cggcaatggc
    19141 aagacaaacc aaacctctat ccgttaagga aatcgaatct gctaaaccca aggaagcgga
    19201 ctacgttctc tatgatggcg atggccttga gctactcatt aaatccagcg ggagtaaaat
    19261 ctggcagttt cgctacattc gccctgtcac caagacacga gcaaagaaaa gcataggccc
    19321 ttacccgtca gttacccttg ccgatgccag aaactaccgt gcagagtccc gctcactcct
    19381 agctaaacag attgatccgc aggaacatca gcaagaacaa ttgcgtagtt cgctggaagc
    19441 caaaaccaat actttccagc tcgtagctga acgttggtgg aatgtgaaga aagccagtgt
    19501 gacagaggac tatgcggaag atatctggcg ctctcttgaa agagatgtct ttcctgcgat
    19561 tggcgacgtt agcgttacag atattaaagc tcatacactg gttcaggccg tacaaccggt
    19621 tcaggccaga ggagcactgg aaacggttcg tcgcctgtgt caacgtatta atgaggtcat
    19681 gatctatgcc cagaacacag ggctgattga tgctgttccc agcgttaata tcggtaaagc
    19741 cttcgaaaag cctcagaaaa agaacatgcc cagcattcga ccggatcagc tatctcaact
    19801 gatgcagaca atgcgaacag ccagcattag cctttccaca cgctgcctgt tcatgtggca
    19861 acttcttact attacccgcc ctgccgaagc ggctgaagct cgctgggaag aagtagacat
    19921 agaagcgcaa gagtggaaga ttcccgcagc acgcatgaaa atgaaccgcg accatactgt
    19981 tccattgtca gatgaagcaa tagctgtgct ggagatgatg aaaccgctaa gcggcaaccg
    20041 agaatttatc tttcctagcc gtatcaagcc caaccagcct atgaacagtc agactgttaa
    20101 cgcctcacta aaacgcgcag gttttggtgg tgtgcttgtt tcgcatggtc tgagatcaat
    20161 tgccagtacg gctcttaatg aacaaggctt tccgcctgat gccattgagg cagcattggc
    20221 ccatgtggat aagaatgagg ttcgtcgtgc ttataaccgc agcgattacc tggaacagcg
    20281 tcgcccgatg atgcaatggt gggcaaattt tgtgatggca gcggatcgtg gaagcatgat
    20341 tgaaggtggg ataaaaggaa tgaagctagt tgggtgacta aatagctgcg cggcatagtg
    20401 tcccactttc agccagccct tgcaggttaa gccaccagac agtaaacggc gggtgtcgcg
    20461 ttattaggcg cagctattta gtagtaatat taggattggt gattctgcac tccagaagtt
//
LOCUS       DAZSNM010000060        20293 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZSNM010000060 DAZSNM010000000
VERSION     DAZSNM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20293
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.060"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(66..824)
                     /gene="aroD"
                     /locus_tag="ACHW42_001801"
     CDS             complement(66..824)
                     /gene="aroD"
                     /locus_tag="ACHW42_001801"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR8943648.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(855..1721)
                     /gene="ydiB"
                     /locus_tag="ACHW42_001802"
     CDS             complement(855..1721)
                     /gene="ydiB"
                     /locus_tag="ACHW42_001802"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR8943649.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1733..3005)
                     /locus_tag="ACHW42_001803"
                     /pseudo
     CDS             complement(1733..3005)
                     /locus_tag="ACHW42_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3188..4393)
                     /locus_tag="ACHW42_001804"
     CDS             complement(3188..4393)
                     /locus_tag="ACHW42_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8943650.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4493..4849)
                     /locus_tag="ACHW42_001805"
     CDS             complement(4493..4849)
                     /locus_tag="ACHW42_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR8943651.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4985..5092)
                     /gene="rprA"
                     /locus_tag="ACHW42_001806"
     ncRNA           complement(4985..5092)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHW42_001806"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5278..6390)
                     /gene="ydiK"
                     /locus_tag="ACHW42_001807"
     CDS             complement(5278..6390)
                     /gene="ydiK"
                     /locus_tag="ACHW42_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR8943652.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6779..9835
                     /locus_tag="ACHW42_001808"
     CDS             6779..9835
                     /locus_tag="ACHW42_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR8943653.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9832..10242
                     /gene="menI"
                     /locus_tag="ACHW42_001809"
     CDS             9832..10242
                     /gene="menI"
                     /locus_tag="ACHW42_001809"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR8943654.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10342..10530
                     /locus_tag="ACHW42_001810"
     CDS             10342..10530
                     /locus_tag="ACHW42_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR8943655.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11078..11446
                     /gene="sufA"
                     /locus_tag="ACHW42_001811"
     CDS             11078..11446
                     /gene="sufA"
                     /locus_tag="ACHW42_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR8943656.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11455..12942
                     /gene="sufB"
                     /locus_tag="ACHW42_001812"
     CDS             11455..12942
                     /gene="sufB"
                     /locus_tag="ACHW42_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR8943657.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12952..13698
                     /gene="sufC"
                     /locus_tag="ACHW42_001813"
     CDS             12952..13698
                     /gene="sufC"
                     /locus_tag="ACHW42_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR8943658.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13673..14944
                     /gene="sufD"
                     /locus_tag="ACHW42_001814"
     CDS             13673..14944
                     /gene="sufD"
                     /locus_tag="ACHW42_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR8943659.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14941..16161
                     /gene="sufS"
                     /locus_tag="ACHW42_001815"
     CDS             14941..16161
                     /gene="sufS"
                     /locus_tag="ACHW42_001815"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR8943660.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16174..16590
                     /gene="sufE"
                     /locus_tag="ACHW42_001816"
     CDS             16174..16590
                     /gene="sufE"
                     /locus_tag="ACHW42_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR8943661.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16739..17743
                     /gene="ldtE"
                     /locus_tag="ACHW42_001817"
     CDS             16739..17743
                     /gene="ldtE"
                     /locus_tag="ACHW42_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR8943662.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17806..18042)
                     /gene="lpp"
                     /locus_tag="ACHW42_001818"
     CDS             complement(17806..18042)
                     /gene="lpp"
                     /locus_tag="ACHW42_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR8943663.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18353..19765)
                     /gene="pykF"
                     /locus_tag="ACHW42_001819"
     CDS             complement(18353..19765)
                     /gene="pykF"
                     /locus_tag="ACHW42_001819"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR8943664.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(19773..19967)
                     /locus_tag="ACHW42_001820"
     CDS             complement(19773..19967)
                     /locus_tag="ACHW42_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8943665.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT         5320 a         4862 c         5177 g         4934 t
ORIGIN      
        1 aaatacggtt aaaaatactt tttttatttc ggcgggggag ggtatttccg ccgaaatatt
       61 attgcttatg cctgatgtaa aatagttaat accgtgcgca aatcatttac cgagatttgc
      121 cctggcgcag aggctttttt taccgcacca aaagttgccg ccgagccaaa tacttcacca
      181 accagacgag aaattacgcc agtttttgcc atcgacatcg tgatgattgg acgatcggca
      241 tactgctcct gcatctccag ggtcgcggca agcaacgtca gcacatcgct ggtactttgc
      301 ggcatcagcg caatcttagg aatatcggcg tcgaaggact gcattttgcg cagacgggca
      361 atgatttctt cggcttccgg cgttttatgg aagtcatggt tggacatgac aactttcaca
      421 tcatgcgcgt gggcgtaggc gacggtttct ttgacctgat catcgccggt aaataactcc
      481 agatcgatca tatcaaccag gccgctgtcg atggctgcac gattgagagc aatataagcc
      541 tcggtggaaa tcgcctgctc gccgccttct ttggcactgc ggaaggtaaa cagcagcggt
      601 ttttctggca tggtttcacg gagaattttt gccgccgcca tgacagactc cacattggag
      661 aggtcggcaa agtggtccac acgccattcc agaatatcaa agtccgcttc acgataggcg
      721 agagcttcgg atttcacgcg ggcgatatct ttcgccatca gcgagacgat gattttaggt
      781 gcgcccgcac caatgacgag atcttttaca gttacggttt tcatttttta ccctttctgc
      841 acgcggtcag cctgtcaggc accgaacccc atgacctgtt taacatattc cagagggaaa
      901 tctttgccag tccataacgt gaactgttca gccccttgcc acaacaacat gccgtatcca
      961 tcaatcgttt tgcaaccagc ttgttgtgcc tgctgcaata acttcgtcat atgcgggtta
     1021 tacacgcatt cagtgaccag cagtcccgga tgtaacagac tgatatcatt aaccaatgat
     1081 tcattctcaa ggggtttcat acctactttt gtgccattgg ttaaaatgtc ggcggaagcc
     1141 agggcttcag caaaggcttg ctgatcggcg agatcggtat ccgtgacgac acaatcggtg
     1201 ttttcgttaa cccgctgcgc gaaggcgagg gctttatcga agaactcatc ccgacggtta
     1261 aagagtttaa tttcttttaa accttcaatt gccccctgcg cgccaatcgc cgttgaggca
     1321 cctccggccc ctaacagcac catcgttttg cctttgatat caaaaccgct ctctttaatg
     1381 gcgcgaatat ggcccgtgcc gtcggtgtta tagccacgca gatagccatc atcattaacg
     1441 atggtgttga tggcacccac cagtttggct gctggcgtta attcatcaac atattcacac
     1501 gccagttgtt tgttcggcat cgacacacca gttccgcgca ttttgagggc ttttaatcct
     1561 tcaattgctc cgggaaagct atcgttatcc acttcgaagg ccatataggt aaatggcaat
     1621 cccgcttttt ctaaggcttt attctgcatt tcaggcgata aactgtggcg gataggataa
     1681 gccatcaatc caatcaattc gtattttgcg gtaacatcca taattaactc ctttaacctc
     1741 tatgcttaat tgtgtttaac cgggtgctaa atttgcgttc gccaaaccgc acatcattct
     1801 caggaataat aaaaacgcgg taatagcggc taaaaacaat aattgcggta atcagggcca
     1861 gcagcgcgaa agtaaaatcg agaacaatga tatattgcag gccgatgttc gacagataac
     1921 cggtaatcag cggaataaca aagttagcca gtccacccat catcatataa atactggtga
     1981 ctttggcttt gcttttgggg aaaaactctg acatgaccga aacaccgagc tgtaaaatgc
     2041 cgccagctgc tgagaaaccg ataacaaagg cgccggcatt acacaccagt ggagaagggt
     2101 acaggtagat aatggcagct gttattgttg ccagcgcaga gttaaataca ttagcccaga
     2161 tgggccggac catttttttc agtagtgcgg caaaaataaa gacacagacc aacgagccca
     2221 tactgtaata agagatggtt tttaatgcct cagcttctga cataccagca aaagccatcg
     2281 catatttggg catccacacc acaatcacat aaaaggtcga gaatgcggct acaccgaaca
     2341 gtaccgatga aacaccttcc agccagacta aaggttttgc tgttcatttg cggtaattca
     2401 ttagctacgc tggcgtccac caactggctg gggaatttgc ttttcaacag catcagcgtg
     2461 atcagtacaa acagaatacc cggaataatc agcccatagc cgtaccagat attattgagc
     2521 aacatatagc tcaccagcat tgggtagaac atttgcccaa atgacaccat cgctttaacc
     2581 agtatgaccg ccgaaccaga ggctttcgga aagcattcca tgagcgcggg gtagccaccc
     2641 gtatccagcg ctgagttagc gatacctacg cacactgcca gaccgtaggc gagagttaaa
     2701 ttcgggcaag cgggaatacc aaagaagaat agcagataca ttattactgc cattaatatc
     2761 accgcccgac gaccaaactt atcggagatc acaccgaaga ataaaatact gatcaatcgc
     2821 cccaaaccga taccggaaat taaataggca atgcccgcgt tgtcagtgga aaacttttct
     2881 gacagagatg tcatattttg ggcaagcgta ataacactaa tgccgtgcag gaaatagctg
     2941 aagtaaatac aaagaacagc caggataaat ggcgtgctga aagataattt cgttaccgta
     3001 cgtattgttc tccgacggtt tacataatta ctatactgaa gatgattttc cctgggtaca
     3061 tggcataaag tttatgtttc caggaatatt gcatgtcata gtttgttttt gatcgcgatt
     3121 ttccttagtt agcgcagaaa gagttattta tccgtaagat cttgcatgac ctgaaaagaa
     3181 tattgcatta cccacccgga gcgacatttt cctttagcga gtgatgccgc gtttttttgc
     3241 ggctgcgtaa gccgataaac agtgccagta aaaaaccgat ggcagtatcg aaccacataa
     3301 tatcggcaat acttctttgg gacagatgag ccgtaatcaa cggaatagta aaggtcgcaa
     3361 tactgcccgt actgtaatag atccctgtag ctttaccttt agcgtaaggg aaacgttcag
     3421 ccattaacgt caggccaatt tgcacaacac ctccagcaga ggtaaaacca atgacaaaag
     3481 caaatattat caccacataa aatgtgggat gcaggcagac ggtaaacaga gcaataaatg
     3541 agataaaggt gtacagcatc agtaatgttg ttgggcgaac ggtattacga atgagtggag
     3601 cggtaataaa tacacaaagc agcgaaccca cggtgtagat actgagtagt ttgatcgaca
     3661 tagtgtatga catgcctgca acaaattgtc cgtactgtgc cagccactgg ctaaccagat
     3721 aaaacgttgc cattgagata tagccatata aggtataact ggctaaatcg ataattgaac
     3781 agcgatgttc cgtagagctg gtggttttct ttatgacagg taagcgacga cccggatggg
     3841 gtgggaacgt acaacggtat aaaaacagag cgttaataaa cataatgcct gcagcaatca
     3901 taaaggacca accgaaccac agttcagccc acactaacag gctaatgatt agcggtaata
     3961 aaaattgtcc gctggaaaca aatgctttaa ttaaaatatt ggctgtccca ggtgagcgtg
     4021 gaaaagcttc catcaaactg ggataagtgc ctgcatcgag aaaactgttt gccattcccg
     4081 ccagaaagcc aaaaacataa gcgataatga tgttattggt ctgcaggatg tcaaaaaaga
     4141 aggccatata gcagcacatc ccgagcatga taaaagggcg gcgaccaaag cgatcggata
     4201 ataatcctgc aaaaagcaag acacttaatc gaccaatgcc cagcgatgag ataactatcg
     4261 agacacccgc ggcattagtc tgccaaagtg tctccagcga ggccatattc aggctcatca
     4321 aaatgacgcc cataccatgc accaggtaat taaaatacaa cccaagtgcg gtagggtaat
     4381 agggattttt cataggttct acttagcaaa ggtgctttaa aaagtgtagc gatgttgtgt
     4441 cagtatccat tcattattgt tgtaacaacg acaattcctt tctgtggaaa aattaacaaa
     4501 gccgctccag atcgtgtgca tataattcag cggctaccga ttgatagatc ttccagtcaa
     4561 taaaattgcc atcagggtag acttgttgaa acgcggttaa gtcggggaaa aaacgcatgg
     4621 tgttgttgcc gatgcggtta tttattttct ctatgatggc atgaagatgt ttttttcgtt
     4681 gctgacgcat agccaacagt tgctcgacta cacaatcagg aatggcgcat ttgccatttt
     4741 cccactgacg ccagctttca ctgtcaccgg tctgggcaat ccaggtcgcg cattcatcaa
     4801 tagtcatggc aaaaatatgg cggagtgcct ggagttcata agcgttcatc gtgtaatggc
     4861 tgcatgaaat aaacaataaa atataaataa cattatcagc ctgctgacgg cttgaagaga
     4921 gtcacagtat cttgtgcaac attattggaa agagtgaggg gcgaggtagc gaagcggaaa
     4981 aatgttaaaa aaaagcccat cgtgggagat gggaaaagac tacacacagc aattcgttgt
     5041 ttcactcagg ggatttccat gcttataaat caatatgttg atttataacc gtgagctaat
     5101 agtaggcata cggacttttt gcgtcgatca gattcgtctc attagtttaa aaactgtaaa
     5161 gaatttgcga caggtactac cagctgaatg gcggtaagca ggaaaatatg gggaagagca
     5221 agcaaatatc aattagcggt gattaacaat caggcggcag agccgcctga tgagaaatta
     5281 cttattcggt ttctcgattt cgccaagttc ttcgagaatt tcttccggtt ggtccgtcgg
     5341 tggcgggact tcttccaccc acgcggcaaa cagacgccag gaaacggcta acagaaccgg
     5401 accaataaac agaccgatca tcccgaaagc aatcaaacca ccaataacgc cagagaggat
     5461 caggatcagc ggtaaatcgg cacccatgcg aattaacatt gggcggatga cgttatccag
     5521 cgtgccaacc acaccgctcc acactaacaa taccgttccc caggtggtat cgccggtcca
     5581 gtagagccag ataatcgccg gaatcagtac cggcaacggg ccaagctgga caaggcagga
     5641 gaggatcatt aacaccgtta gcaacgttgc ataaggtacg ccggatacgg cgaggccgat
     5701 accgccaagc actgcctgta ctaacgccgt caccaccaca cccagcgcca ccgcgcggat
     5761 agcctgtgcc gccagcagca cggcggcatc tccgcgaacg cctgccagac gggttgcaaa
     5821 atggcgaatg ccttgtgcca cctgttcacc gcgccagtac agcagggcac tgaagagaag
     5881 catcagcgca caatgcacca taaagcgccc gatatgcgcc gcctgcccaa cgaaccaggt
     5941 ggtggtggtg ccaatataag ggcggacttt cgccatgatc gccgtacccc ccatatccag
     6001 caagttgtgc cagcctgcat acagcttcgc gccaatcacc ggaatggtat taagccacgc
     6061 cagatcgggt aacgtcatgt cgccgctgga aatggcttta attagcgggc cgctgccgtc
     6121 gacgatactg ttaaccagca aagcaatagg gatgataaac accatcacta ataacagcgt
     6181 catcaccaga acggcgagag agcggcggcc aaacatgatc ttttgcaaac gtaacaatac
     6241 cggccaggtg gcgataacca ccgtaccggc ccatgcaaag ccgagaataa agggttgaac
     6301 aatccacaga catgccacaa tcatgatggc taaaaacagc accgaaagca gaatttgtgc
     6361 gacatccctg ggctgacgaa catttaccat aactactttt cacctttttt gtgcgtcaaa
     6421 tcgtcgacgc gacgtgaaca cgcgaaactc acctgcataa tgatgagcaa tttcagcggt
     6481 ttttaacagg ccgattctgc ccttaattct gatgggcgtg ttagaaaaaa atgtggtaca
     6541 acaactcagg caacacgcaa acgatttact cgtcgtattt caagttgcat gtgctgcggc
     6601 tacgttcgtt caccccagtc acttactatt gtaagctcct ggagttcact cgcttgccgc
     6661 cttcctgcaa ctcgaaatcc atagagtaaa taaacatcac ctttcagtgc gctattgcag
     6721 ccgcctgaaa gaatagtgta tcgacctaca tcacagaccg caggaaaggg tcaatataat
     6781 gattccacag atttccctgg cacccggcgt cgttcaactg gtgcttaatt ttttgcaaga
     6841 gctggagcaa caaggtttta ccggcgatgc ggcgacaagt tatgccgatc gtctgacaat
     6901 gtcgaccgac aacagtattt accaacttct ccccgatgcg gtggtatttc cgcgttcaac
     6961 cgcagatgtg gcgctgatcg cccgtcttgc cgcgcaggaa cgctattcat cgctgatctt
     7021 taccccccgc ggcggcggca ccggcactaa cggtcaggcg ctcaaccagg ggattattgt
     7081 tgatatgtcc cgccatatga accgcatcat cgaaattaac cctgaagagg gctgggtgcg
     7141 cgttgaggcc ggggtgataa aagaccaact caatcagtac ctgaaaccgt tcggctactt
     7201 ttttgcgccg gaactttcga ccagcaaccg ggcaacgctc ggtgggatga tcaataccga
     7261 tgcatccggt cagggatcgc tggtctatgg caaaacgtca gatcacgtac ttggcgtacg
     7321 cgcggtgttg ttggggggcg atattctcga tacgcaacct ttacccgtcg agctggcgga
     7381 aacgctgggt aaatccaata ccacaatcgg gcgaatttat aacacggttt atcaacgttg
     7441 ccgtcagcaa cgccagttaa tcatcgacaa cttccccaaa cttaaccgct ttcttaccgg
     7501 ttacgatctg cgtcatgtct ttaacgatga gatgaccgag ttcgacctga cgcgcattct
     7561 gacgggttca gaagggacgc tggcctttat taccgaagcg cggctggata ttacgcgctt
     7621 gcctaaagtg cgccgtctgg tgaatgtcaa atatgactct tttgactctg cgctgcgtaa
     7681 cgcgccgttt atggttgagg cgcgggcgct ttcggtagag acggtggact caaaagtgct
     7741 gaatctggcg cgggaagata ttgtctggca ttccgtcagc gagttgatta ccgatatgcc
     7801 tgacaaagag atgctcggac tgaacattgt ggaatttgct ggtgatgatg aggcgctgat
     7861 tgacgagcga gttaatgcac tctgtgtgcg gcttgatgag ctgattgcca gccaacaggc
     7921 aggtgtgatt ggctggcagg tgtgccgcga gctggcgggg gttgaacgta tctatgcgat
     7981 gcgcaaaaaa gccgttggtc tgcttggcaa tgccaaaggt gccgctaagc caattccgtt
     8041 tgctgaggat acctgcgtac cgccggaaca tctggccgat tatattgctg aatttcgcgc
     8101 gctgctcgac agccacggct taagctacgg tatgttcggt cacgtcgacg caggtgtctt
     8161 gcacgtccgt ccggcactgg atatgtgcga tccgcaacaa gagattttga tgaagcaaat
     8221 ctctgatgac gtggtggcgc tgactgcgaa atacggtggt ttgttgtggg gcgagcacgg
     8281 caaaggtttt cgcgctgaat acagcccggc gtttttcggt gaggaacttt ttgcagaact
     8341 gcgcaaagtg aaagcggcat ttgacccgca taaccgactc aacccaggga agatttgccc
     8401 gccagaaggt ctcgatgcgc cgatgatgaa agtggacgcg gtgaagcgcg gtacattcga
     8461 tcggcagatc cccattgcgg tacgccagca gtggcgcggt gcgatggagt gtaacggcaa
     8521 cggtttatgc ttcaactttg atgcccgtag tccgatgtgt ccgtcgatga agatcaccca
     8581 gaaccggatt cattcaccga aagggcgcgc aacgctggtg cgtgaatggc tgcgcttgtt
     8641 ggcggatcgc ggcgttgacc cactcaaact ggaacaagaa ctgcctgaat ccggcgtcag
     8701 tttgcggacg ttaattgccc gcacgcgcaa tagctggcat gcgaataaag gcgaatatga
     8761 cttctcacac gaagtcaaag aggcgatgtc gggctgtctg gcctgtaaag cttgttcgac
     8821 ccagtgcccc atcaaaattg atgtgccgga gtttcgctct cgttttctgc agctctatca
     8881 cacccgttat ttacgcccgc ttcgcgacca cctcgttgct acggtcgaga gctacgcacc
     8941 gctgatggca cgcgcgccga aaacctttaa cttcttcatt aaccagccgc tggtgcgcaa
     9001 actctcggaa aaacatatcg gcatggttga tttgccactg ctgtcggtcc cctcgctaca
     9061 acaacaaatg gtggggcatc gctcggcaaa catgacgctg gaacagcttg aagctctcaa
     9121 tgcagagcag aaagcgcgca cggtgttggt ggtgcaggac ccctttacca gctattacga
     9181 tgcgcaagtg gtggcggatt ttgtccgtct ggttgaaaaa ttaggtttcc agcctgtgtt
     9241 actgccattt tcgccaaatg gcaaagccca gcatatcaaa ggctttctta atcgttttgc
     9301 gaagacggcg aaaaagacgg cggatttcct caaccgtatg gctaagcttg ggatgccaat
     9361 ggtgggcgtc gatccggcgc tggtactttg ttatcgcgat gaatataaac tggcgctggg
     9421 cgaggaacgt ggcgagttta acgtcttact tgcgaatgaa tggctggcaa gcgcacttga
     9481 gtcacatcca gtggctacag tcagcggtga atcatggtat ttctttggtc actgtaccga
     9541 ggttaccgct ttgccgggcg cgccagcaca atgggccgcg atatttgccc gttttggcgc
     9601 gaaactggaa aatgtcagcg tgggttgctg cggcatggca gggacttacg gacatgaagc
     9661 gaaaaaccat gaaaattcgc tcgggatcta tgagttatcc tggcatcagg ctatgcagcg
     9721 actgccgcgt aaccgctgtc tggcgaccgg atattcctgc cgtagccagg taaaacgggt
     9781 tgaaggcacg ggggtacgcc atcctgtgca ggctttactg gagattatta aatgatatgg
     9841 aaacggaaaa tcaccctgga agcactgaat gctatgggtg aaggaaacat ggtggggttc
     9901 ctggatattc gctttgaaca tattggtgat gacacccttg aagcgacaat gccagtagac
     9961 tcgcggacaa agcagccttt cgggttgctg catggaggag catccgtggt actggccgaa
    10021 agtatcggtt ccgttgccgg ttatttatgt accgaaggtg agcaaaaagt ggttggtctg
    10081 gaaatcaatg ctaatcacgt ccgctcggca cgagaagggc gggtgcgcgg catatgtaaa
    10141 ccgttgcatc tcggttcgcg tcaccaggtc tggcagattg aaatcttcga tgagaaaggg
    10201 cgtttgtgct gttcgtcacg attgacgacc gccattttgt gacgttgtca ccagaaaagt
    10261 gtgacgcata ttgcttttgg tcattatctg cacagtaaac ggtggtatac tgtgcaggtc
    10321 ttgtctaaaa gcgaggatgc tatgtctact caacttgatc ccacccagct ggccattgaa
    10381 tttttacgcc gtgaccagag caatctttca cctgcccaat atctcaaacg tctgaaacaa
    10441 ctagaacttg aatttgctga cctgcttact ctctcttctg ctgaattaaa agaagaaatc
    10501 tacttcgcct ggcgtctggg cgtgcattga tcctgactta atgccgcaag ttcgccaaat
    10561 cgacctggat taacctccgt ccaggttcga tgaccttctt aatactgctt atctaccgcc
    10621 tgatattgtt caaaaataat actaatcgca gtttgtgtta aaacggcggg ttagctttat
    10681 gagattattc ttatcgcccc ttcaagagct aagccactga gagtgccgga gataagcgcc
    10741 ggatggggta gaaaccctta agcctgtgtc gcacagactt aagggttttc ttatttctga
    10801 acgctgcact tttttaccta tctcttaaat agtcattttc aatacaactt tttctttttc
    10861 atcacagcct taatgctcct tagataataa ccattatcta acaatgagat acctaattct
    10921 tagcgtgcct gttaacccac acatcagggg ctatgcttat taaaagcagg caaaaagaat
    10981 gttttacggt aaagcccctg cgtttgctgg gttgaactga taatcattat cactaacatg
    11041 ctgttatacg ctgaaagcga tgaagtgagg taaatagatg gacatgcatt caggaacctt
    11101 taacccacag gatttcgcct ggcaaggctt aacgctgaca cccgcagcgg cgatacacat
    11161 ccgcgagctg gtggcaaagc agccaggtat ggtcggcgtg cgcttaggtg tgaagcaaac
    11221 gggctgcgcg ggctttggct atgtgctcga cagtgttagc gagccggaca aagacgatct
    11281 gctgtttgaa cacgacggcg cgaagctgtt tgtcccgctg caatcgatgc cgtttattga
    11341 tggcacggaa gtcgatttcg ttcgtgaagg acttaatcag atattcaaat ttcacaaccc
    11401 taaagcccag aatgaatgtg gctgtggcga aagctttagg gtataggcgg tactatgtct
    11461 cgtaatactg aagcaactga cgatgtcaaa acctggaccg gcggcccgct gaactataaa
    11521 gaaggattct tcacccagtt agccaccgat gagctggcaa aggggataaa cgaagaggtg
    11581 gtgcgcgcaa tttcggcgaa gcgtaatgag ccggagtgga tgctggagtt tcgtctaaac
    11641 gcctatcgcg catggctgga gatggaagaa ccgcactggt tgaaagcgca ctacgacaag
    11701 ctgaattatc aggattacag ctactactca gcaccatcgt gcggtaattg tgacgacact
    11761 tgcgcgtctg aacctggcgc ggtgcagcaa actggcgcga acgccttttt aagtaaagag
    11821 gtggaggcgg cgtttgagca gttgggcgtt cccgtgcggg aaggcaaaga ggtggcggtg
    11881 gatgccattt tcgactctgt ttcggttgcc accacttatc gcgaaaaact ggcggagcag
    11941 ggaattattt tctgttcctt tggtgaggcg atccacgatc acccggaact ggtgcgtaaa
    12001 tatctcggca ccgtggtgcc ggggaatgac aacttctttg cagcacttaa tgcggcggta
    12061 gcctctgacg gtacgtttat ttatgtgcct aaaggcgtgc gctgcccgat ggaactttcc
    12121 acctattttc gcattaacgc ggaaaaaacc gggcagtttg agcgcacaat tctggtggcc
    12181 gacgaagaca gctacgtcag ctacattgaa ggctgttccg ctccggtgcg tgacagctat
    12241 cagttgcacg cggcagtggt ggaagtcatc atccataaaa acgccgaggt gaaatattcc
    12301 acggtacaaa actggttccc tggcgataac aacaccggcg gtattctcaa cttcgtcacc
    12361 aagcgtgctt tgtgcgaagg cgaaaacagc aaaatgtcat ggacgcaatc agaaaccggg
    12421 tcagcgatta cgtggaaata tcccagctgc attttgcgcg gcgataactc cattggtgag
    12481 ttttactcag tggcgctgac cagcggtcat cagcaagcgg ataccggcac caagatgatc
    12541 cacatcggta aaaacaccaa atcgaccatt atctcgaaag ggatctctgc cggacatagt
    12601 cagaacagtt atcgcggctt agtgaaaatc atgccgacgg caaccaatgc gcgcaatttc
    12661 actcagtgcg actcaatgct gattggcgct aattgtgggg cgcatacctt cccgtatgtc
    12721 gaatgtcgca ataacagcgc acaactggag cacgaggcaa cgacatcacg tattggtgaa
    12781 gatcaactgt tttactgcct gcaacgcggg atcagcgaag aagacgccat ctcgatgatt
    12841 gttaacggtt tctgcaaaga cgtgttctca gaactgccgc tggaatttgc cgttgaagca
    12901 caaaaactcc tcgccatcag tcttgaacac agcgtcggat aaggaataaa catgttaagt
    12961 attaaagatt tacacgtcag cgtggaagat aaagctatcc tgcgtggatt aagcctcgac
    13021 gttcgtcccg gcgaagttca cgccattatg gggccaaacg gttcgggcaa aagtacctta
    13081 tcggcaacgc ttgccgggcg cgaagattat gaagtgacgg gcggcacggt tgagttcaaa
    13141 ggcaaagatt tgcttgcact gtcgccggaa gatcgtgcag gcgaaggcat ctttatggcc
    13201 ttccagtatc cggtggagat tccaggcgtc agtaaccagt ttttcctgca aacggcactt
    13261 aatgcagtgc gcagctatcg cggccaggaa acgctcgacc gctttgattt tcaggatttg
    13321 atggaagaga aaatcgctct cctgaagatg ccggaagatt tattaacccg ttcggtaaac
    13381 gttggttttt ccggcggcga gaaaaagcgc aacgatattt tgcaaatggc ggtgctggaa
    13441 ccggagttat gcattcttga tgagtcggac tccgggctgg atattgacgc attaaaagtg
    13501 gtcgccgatg gcgtgaactc gctgcgtgat ggcaagcgct cattcatcat tgttacgcac
    13561 taccaacgca ttctcgacta catcaagcct gattacgttc atgtgctata tcagggacga
    13621 attgtgaaat ccggcgattt cacgttggtc aaacaactgg aggagcaggg ttatggctgg
    13681 cttaccgaac agcagtaatg cgctgcaaca gtggcatcac ttgtttgaag ctgaagggac
    13741 aaaacgctca ccgcaagcac agcagcattt acaacaattg ctgcgtaccg gactgccgac
    13801 acgtaaacat gaaaactgga aatatacgcc gctggaaggg ctgaccaata gtcagtttgt
    13861 cagcattgcg ggagatatat ccccacagca gcgtgatgcc ttagcgttaa cgttagacgc
    13921 cgtgcggctg gtgtttgtcg atggacgtta cgtgcccgcg ctgagcgatg caattgaagg
    13981 cagcggatat gaagtgagca ttaacgacga ccgtcagggg ttacccgacg ctattcaggc
    14041 ggaagtgttt ctgcatttga cggaaagcct ggcacaaagc gtgacgcata tcgccgtgaa
    14101 gcgcggtcaa cggccggcaa agccattgct gttaatgcat atcacccagg gcgtggcagg
    14161 tgaagaggtg aacactgccc attaccggca tcatctggat ctggcggaag gtgccgaagc
    14221 aacggtgatc gaacattttg tcagccttaa tgatgctcgt cattttaccg gggcacggtt
    14281 cactatcaat gtcgcagcga acgcccactt gcagcatatc aagctggcgt ttgaaaaccc
    14341 ggtcagtcac cactttgccc ataacgattt gttgctggct gacgatgcca ccgcatttag
    14401 ccatagtttc ctgctgggtg gcgcagtgtt acgacacaac accagtacgc aactcaatgg
    14461 cgaaaacagc acgctgcgga tcaatagcct ggcgatgccg gtgaaaaacg aggtgtgtga
    14521 tacccgtacc tggctggaac acaataaagg tttttgtaac agccgacagt tgcacaaaac
    14581 tatcgtcagc gacaaaggcc gcgcggtatt taacggtttg atcaacgtcg cgcagcacgc
    14641 catcaaaacg gatggtcaga tgaccaacaa taatctgctg atgggcaaac tggcggaagt
    14701 ggatacaaaa ccgcagctgg aaatctatgc agatgatgtg aaatgcagcc acggcgcgac
    14761 ggtggggcgt attgatgatg aacagatgtt ctatctgcgc tcgcgcggga tcaatcagca
    14821 ggatgcccag cagatgatca tttacgcctt tgctgccgaa ctgacggaag cactgcgtga
    14881 tgaggggctt aaacagcagg tgctggcccg aatcggtcaa cggctgccag gaggtgcaag
    14941 atgacttttt ccgtcgacaa agtgcgggcc gactttccgg tgctttcgcg tgaggtaaac
    15001 ggtttgccgc tggcttatct cgacagcgcc gccagtgcac agaaaccgag ccaggtgatt
    15061 gacgccgagg ccgagtttta tcgtcatggc tacgcggcgg tgcatcgcgg tattcatacc
    15121 ttaagcgccc aggcgaccga gaaaatggag aacgtgcgca agcgggcatc gctgtttatt
    15181 aatgcccgtt cggcggaaga gctggtgttt gtccgcggca cgacggaagg gatcaatctg
    15241 gtcgccaata gctggggcaa cagcaatgtg cgggcgggcg ataacatcat catcagtcag
    15301 atggagcacc acgctaacat tgtcccctgg cagatgcttt gcgcacgcgt tggcgcagag
    15361 ctgcgtgtga tccctctcaa ccccgacggt acgctgcaac tggagacgct gcctacgctg
    15421 tttgatgaga aaactcgcct gctggcaatt actcatgtct ccaacgtgct tggcacagaa
    15481 aatccactgg cggaaatgat cacgcttgcg caccagcatg gcgcaaaagt gctggtggat
    15541 ggcgctcagg cggtgatgca tcatccggtg gatgttcagg cgctggattg cgacttttac
    15601 gtgttctccg ggcataaact gtatggcccc accggaattg gcattcttta tgtgaaagaa
    15661 gccttgttgc aggagatgcc gccgtgggaa gggggcggtt ctatgatcgc caccgtcagc
    15721 ctgagtgaag gcactacctg gaccaaagca ccatggcggt ttgaagccgg tacacccaat
    15781 accgggggca tcattggtct tggcgcggcg ctggagtatg tttcggcgct ggggcttaat
    15841 agcatagccg agtatgaaca gaatctgatg cattatgcgc tatcacagct ggaatctgta
    15901 ccggatctca ctctctatgg ccaacaaaac aggcttggcg ttattgcttt taatctcggt
    15961 aaacaccacg cctatgatgt tggcagtttt ctcgataatt acggcattgc tgtgcgtacc
    16021 ggacatcact gcgctatgcc attaatggcc tattacaacg tccctgcgat gtgtcgggcg
    16081 tcgctggcca tgtataacac ccatgaagaa gtggagcgtc tggtgaccgg cctgcaacgt
    16141 attcaccgtc tgctgggata acagggaggc actatggctt tattgccgga taaagaaaaa
    16201 ttgctgcgta attttttacg ctgcgccaac tgggaagaga aatatctcta cattattgag
    16261 ctgggccagc gtctgccaga attacgcgcc gaagacggaa gcccacaaaa tagcattcag
    16321 ggctgccaga gtcaggtgtg gattgtcatg cgccagaatg cgcagggaat tattgaattg
    16381 cagggcgaca gcgatgcggc gattgtgaaa gggcttattg cggtcgtctt tattctctac
    16441 gatcagatga cgccgcagga tattgtcaat ttcgatgtgc gtccgtggtt tgaaaaaatg
    16501 gcgctcaccc aacatctcac cccatctcgt tcacaaggtc tggaagcgat gattcgcgca
    16561 attcgcgcca aagccgctgc acttagctaa actaaaggga cagctttcat ccggttggct
    16621 cacattgccg gatggtcaat gtttacactg accggcacag gagtgttttg gtttcaggtg
    16681 aacataagca ccatccgacg tttcagcgtg agtctccggc aaatacagga ggtttacaat
    16741 gaaacgcgcg tctttgctta cactcacgct tatcggcgct tttagcgcca tccaggctgc
    16801 ctgggcggtt gattatccgc taccaccaac cggaagccga ctggttgggc aaaatcaaac
    16861 gtatacggtg caagaagggg ataaaaacct tcaggctatt gcccgacgtt ttgatactgc
    16921 agcaatgttg atccttgaag ccaataacac tatcgccccg gtgccaaaac ctggtacgac
    16981 gataactatt ccttcacaac tgttattacc tgatgcaccg cgtcagggga ttatcgttaa
    17041 ccttgcagag ctgcgccttt attattatcc gccgggagaa aatattgtgc aggtttatcc
    17101 aataggtatt ggattgcagg ggctggaaac gccggtgatg gaaacgcgtg ttgggcagaa
    17161 aatccctaac ccaacctgga cgcctacggc aggcattcgt cagcgttcgc tggagcgtgg
    17221 cattaaatta ccgccagtcg ttcctgccgg accaaataac ccgctaggac gttacgcact
    17281 gcgcctcgcg catggtaatg gcgaatacct cattcatggc accagtgcgc cggacagcgt
    17341 tggtttgcgc gtcagttcag ggtgtattcg catgaatgca ccggatatta aagccttgtt
    17401 ctccagcgtg cggacgggaa cgccggtgaa agtgatcaac gaaccggtga aatattccgt
    17461 ggaacctaac gggatgcgtt atgttgaagt acatcgacca ctatcagcag aagaacagca
    17521 aaacgttcag acaatgccat acacactgcc agcaggcttt acgcaattta aagacaataa
    17581 ggctgtagat cagaagttag tcgataaagc gttgtatcgt cgggcagggt atccggtttc
    17641 ggtgagcagt ggagcaactc ccgcagccag caatgcgcct tcagtagagt cagcgcagaa
    17701 tggtgaacca gagcaaggga atatgttacg cgcgacgcag tagtggtaaa tagcagacaa
    17761 aaaaatggcg cacaatgtgc gccatttttc acttcacagg tactattact tgcggtattt
    17821 agtagccatg ttgtccagac gctggttagc acgagctgcg tcatctttag cagcctgaac
    17881 gtcggaacgc attgcgttca cgtcgttgct cagctggtca actttagcgt tcagagtctg
    17941 aacgtcagaa gacagctgat cgattttagc gttgctggag caacctgcca gcagagtaga
    18001 acccaggatt accgcgccca gtaccagttt agtagctttc attattaata ccctctagat
    18061 tgagttaatc tccatgtagc gttacaagta ttacacaaag ttttttatgt tgagaatatt
    18121 tttttgatgg gaaggcactt atttttgatc gttcgctcaa agaagcatcg aacgctgggt
    18181 tttagataaa aaaattgaga gaaaacagga tgcttccatc ggattcatct taaataaagt
    18241 gagattatat agtgaaatcc gatttgattt tttattgctt ctggttatcg attaaataaa
    18301 aaaagcgccc atcagggcgc ttcgatatac aaattaattc acaaaagcaa tattacagga
    18361 cgtgaacaga tgcggtgtta gtagtgccgc tcggtaccag tgcaccagaa accataacta
    18421 cgacgtcacc tttgtgtgcc agaccgctct gcagagccag ttctttaccc agacggtaga
    18481 aatcatcagt agaagtgatc tctttaacca gctgcggcac aacgcctttg ctcagtacca
    18541 actgatgagc cgttttttcg ttggtggtca gtgccaggat ggtggcatcc gggaagtatt
    18601 tacgtactgc gcgagcagat ttaccgccct gggtagcaac cacgatcagc ggagcatcca
    18661 gtttttcagc agtttcaacg gcaccacggc ataccgcttc ggtgatgcgc agtttacggt
    18721 tgtcattgtt gaactcgaga cggctgttca tcacgcggtc ggtacgttcg cagatggtcg
    18781 ccatgataga aaccgcttcc agcgggtatt taccttttgc ggattcacca gacagcatca
    18841 ctgcgtcagt accgtcgagg atggcgtttg caacgtcacc ggcttctgcg cgagtcgggc
    18901 gtgggttttt gatcatggaa tccagcatct gggtcgcagt gataacgact ttacgtgcac
    18961 ggatacattt ttcgatcatc atcttctggg cgaagataac ttcttctacc gggatttcta
    19021 cacccaggtc gccacgcgca accatgatgc cgtcagaggc ttcgaggatt tcgtcgaagt
    19081 tgttgaggcc ttcctggttt tcgattttgg agatgatgtg gatgttttcg ccgccgtgcg
    19141 ctttcaggtg ctcacggatt tcgataacgt cagaacgctt acgaataaag gaagcagcaa
    19201 caaagtctac gcctttttcg caaccaaaga tcaggtcctg tttgtctttt tcagccagtg
    19261 ctggcagagc aatggaaacg ccaggcaggt tcacaccttt gttttcgccc aggtcaccgt
    19321 tgttcagcac tttacagata actttgttac cttcaatggc ggtaacttcc ataccgatca
    19381 gaccatcgtc aaccagtacg gtgttgccaa cagacaggtc agtagtgaaa ccttcatacg
    19441 ttaccgcaac catttcgctg ttgccgataa cagatttatc agtggtgaaa gtaaaggtct
    19501 gaccagcttt cagagaaaca tcgttaccgc cttccagttt catggtgcgg atttccggac
    19561 ctttggtatc aagcaggata gcggcggttt taccagtttt gctcatcacg ttgcgcagat
    19621 tctgaatgcg ctgaccgtgt tctgcatagt caccatgaga gaagttcaga cgcataacgt
    19681 tcatgccagc gtccagcatt ttagctaaca tctcttcaga ttcggttttc ggtccgatgg
    19741 tgcaaacaat tttggtcttt ttcatgacag tcttagtctt taagttgaga aggatgggag
    19801 aaacttgctt tctgggcgca ctgccggaaa gcgaatccgg tattacgaaa gtggtggtgc
    19861 ctcgctctaa ggataggtga catcgaaaaa gcgtgcagag gaatgtgtgc ttgtgtctca
    19921 gcccaacgtg agtgtctgtt tttgagtcgt tgagttctac agtccaaagt gctgaaacca
    19981 ttcaagagtc aattggcgcg cattatacgc tgaaattaat ccaaaaggaa aacaaaaaca
    20041 gcgtttcaaa aaatatacaa cttgttagac aataaattgt tatcaaagat ttaacgttcc
    20101 agggaaaagg ttacgcaaaa acttcgctgt ttgcgcaacg tctggtgccg aagttgtcag
    20161 tgtgaggata agtgaaagtt catccggtgt ataaatatat catcgctcaa ttgctacagt
    20221 taaaataatt tatcgcgtta tttgtctgat tttgtttgat ttttgtttaa ttggttagat
    20281 tgatatatgc tga
//
LOCUS       DAZSNM010000061        20084 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZSNM010000061 DAZSNM010000000
VERSION     DAZSNM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20084
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.061"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="ACHW42_001821"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="ACHW42_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR8943666.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACHW42_001822"
     CDS             complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACHW42_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsaE"
                     /protein_id="HFR8943667.1"
                     /translation="MRNAVSKAGIISRRRLLLFQFVG"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACHW42_001823"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACHW42_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR8943668.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="ACHW42_001824"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="ACHW42_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="ACHW42_001825"
     CDS             1631..2341
                     /locus_tag="ACHW42_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR8943669.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACHW42_001826"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACHW42_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR8943670.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4129)
                     /locus_tag="ACHW42_001827"
                     /pseudo
     CDS             complement(3469..4129)
                     /locus_tag="ACHW42_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
     gene            4282..6480
                     /locus_tag="ACHW42_001828"
     CDS             4282..6480
                     /locus_tag="ACHW42_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFR8943671.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6449..6889)
                     /locus_tag="ACHW42_001829"
     CDS             complement(6449..6889)
                     /locus_tag="ACHW42_001829"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR8943672.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6886..7449)
                     /gene="tag"
                     /locus_tag="ACHW42_001830"
     CDS             complement(6886..7449)
                     /gene="tag"
                     /locus_tag="ACHW42_001830"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR8943673.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7607..8305
                     /locus_tag="ACHW42_001831"
     CDS             7607..8305
                     /locus_tag="ACHW42_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR8943674.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8534..9742
                     /locus_tag="ACHW42_001832"
     CDS             8534..9742
                     /locus_tag="ACHW42_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8943675.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10066..11757
                     /gene="eptB"
                     /locus_tag="ACHW42_001833"
     CDS             10066..11757
                     /gene="eptB"
                     /locus_tag="ACHW42_001833"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR8943676.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11849..11925
                     /locus_tag="ACHW42_001834"
     tRNA            11849..11925
                     /locus_tag="ACHW42_001834"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11883..11885,aa:Pro,seq:cgg)
     gene            12669..14276
                     /gene="dppA"
                     /locus_tag="ACHW42_001835"
     CDS             12669..14276
                     /gene="dppA"
                     /locus_tag="ACHW42_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR8943677.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14584..15603
                     /gene="dppB"
                     /locus_tag="ACHW42_001836"
     CDS             14584..15603
                     /gene="dppB"
                     /locus_tag="ACHW42_001836"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR8943678.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15613..16515
                     /gene="dppC"
                     /locus_tag="ACHW42_001837"
     CDS             15613..16515
                     /gene="dppC"
                     /locus_tag="ACHW42_001837"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR8943679.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16526..17509
                     /gene="dppD"
                     /locus_tag="ACHW42_001838"
     CDS             16526..17509
                     /gene="dppD"
                     /locus_tag="ACHW42_001838"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR8943680.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17506..18510
                     /gene="dppF"
                     /locus_tag="ACHW42_001839"
     CDS             17506..18510
                     /gene="dppF"
                     /locus_tag="ACHW42_001839"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR8943681.1"
                     /translation="MSTQETTSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18540..19811)
                     /locus_tag="ACHW42_001840"
     CDS             complement(18540..19811)
                     /locus_tag="ACHW42_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR8943682.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4814 a         4974 c         5274 g         5022 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggcaggc
     4561 ggttataccg ggcatctggg ggattattcg accggtgcgg cacaggcgat catgccgtat
     4621 gtcgtaggtg gcagcgaagt ttatcaacag caaaccagtt ggccgctggt gctggaacat
     4681 agcgatgtcg tggtgttgtg gagtgctaac ccactcaata cgctgaaaat tgcgtggaat
     4741 gcatccgatg agcaggggct ttcttacttt tccgcactgc gtgacagtgg gaaaaagctg
     4801 atctgcattg atccaatgcg atcggaaacc gtcgatttct ttggcgataa aatggagtgg
     4861 gtggcaccgc acatgggcac cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg
     4921 gaaaatggtt ggcacgacga agcgtttctg gcgcgttgca ccacaggtta tgccgtcttc
     4981 gcctcttatt tgctgggcga gagtgacgga atagcgaaaa acgccgaatg ggcggcagag
     5041 atttgtggtg ttggcgcagc gaaaatccgc gagctggcgg ctattttcca ccaaaatacc
     5101 accatgctga tggcaggctg gggaatgcag cgccaacagt ttggtgagca aaaacactgg
     5161 atgatcgtca cgctggcagc aatgttgggg caaatcggca cacccggcgg cggttttggt
     5221 ctttcttacc attttgccaa tggtggtgac cccacgcggc gttctgcggt gctctcttcc
     5281 atgcagggca gcttgccggg tggctgcgat gcggtggata aaatccctgt tgcccgcatt
     5341 gttgaagcac tggaaaaccc tggtggcgca tatcaacaca acggtatgaa ccgacatttc
     5401 ccggatattc gttttatctg gtgggcgggc ggtgccaact ttactcatca tcaggatacc
     5461 aatcgtctga tccgtgcctg gcaaaaaccg gagctggtgg tgatctctga atgcttctgg
     5521 acggcggcgg cgaaacacgc ggatattgtt ctgcctgcga ctacctcttt tgagcgtaat
     5581 gatctcacca tgaccggtga ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg
     5641 ccgccacgct atgaagcgcg taatgatttt gatgtttttg ccgagttgag tgaacgttgg
     5701 gagaagggcg gctatgcgcg gtttacggaa ggaaaaagtc agctgcaatg gctggaaacg
     5761 ttttataacg ttgcccggca gcgcggggca agccagcagg ttgaattgcc gccatttgct
     5821 gagttctggc aagccaacca gttaattgag atgccggaaa acccggacag cgagcggttt
     5881 attcgcttcg ctgatttttg ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc
     5941 aagattgaaa tcttctcaca gcgtattgcc gattacggtt acccggattg ccctgggcat
     6001 ccaatgtggc tggagccgga cgaatggcag ggcaatgccg aaccagaaca gttgcaggta
     6061 ctttctgccc atccggcgca ccgcctgcac agccagctga attacagttc tctgcgcgaa
     6121 ttgtacgcgg tagcaaaccg tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc
     6181 ggcataaccg aaggggatat ggtgcgggtg tggaactccc gtgggcagat ccttgccgga
     6241 gcggtcatta gcgagggaat taaacctggc gtgatttgca ttcacgaagg ggcatggccg
     6301 gatctggatt taaccgctga cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa
     6361 gatctcccca gctcgcggct ggggaatggc tgtgcgggta atacgacgct ggcatggctg
     6421 gaaaaataca acggtccgga actgacactt acagcgtttg aaccaccggc cagctcataa
     6481 tccaggtggg tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg
     6541 cacggtaaaa atctatcgcc ggttgatttt tttgataaac ctccagcatc agatggggat
     6601 agcgctgctg cacatactgc atcagcgcct taccaatacc gcgtctgacg gccttcggtg
     6661 cgacaaacat tgctgccaga aatcggcctt ccataatgct gacaaaaccg agaagcttac
     6721 cgtcctcttc ccagacccag ttttgcgcgt tggcaagata ggcatcccgc accagcggaa
     6781 tgcagtcacg ccagtaattc gcttttataa agggatgccc ccaggttgta ctttccagcc
     6841 acagttcgag gatcgcgggg agttctgaac gttgcgcttc ccgaatcatg gtttatttcc
     6901 cagatagcaa cagcagccaa ccacatgatc attcaccagc ccacatgcct gcataaagga
     6961 gtaacagatt gtggtgccga caaacttaaa accacgtttt ttcagtgcct tagatagggc
     7021 gtcggaggcg gacgtagatg tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg
     7081 ctgatgattt acaaacgacc agacaaagtc gggaaacggt tcgccgttct gttccatttg
     7141 caggtacgcc cgcgcattac caataattgc ctgaattttc cctcgatggc ggataatccc
     7201 ggcgtcctgt accagtcttt cgacatcctc ttcctgcatt gctgcgacct tcaccggatc
     7261 gaactgatga aagtaggcgc gatagttttc gcgttttttg aggacggtga tccacgataa
     7321 cccagcctgc tgcccttcaa agcagatcat ttcgaacagt tttttactgt cagtttcagg
     7381 cacgccccac tcattatcat ggtaggcaat ataaagcggg ccctgactca cccagccgca
     7441 acgttccata ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg cttttcttga
     7501 cgcatcggtg aaaaagtcaa tatttaatac agggctacta tgcccgatat ccttcataca
     7561 atgacaataa tattgccgaa ttcggctctt ccctggagtc gtgttaatga tcataaaaaa
     7621 aagcggtggt cgctggcagc taagcctgct ggcgagcgtg gtaatcagtg ccttttttct
     7681 caacacagct tacgcctggc aacaagaata tatcgttgat acgcaacccg gacattccac
     7741 agagcgttac acctgggata gtgatcatca acctgattac aacgatattt tgtcgcaacg
     7801 tattcaaagt agccaaaggg cgctgggact ggaagtcaat ctggcggaag aaactcctgt
     7861 ggatgtgacc agcagtatga gtatgggctg gaattttcct ttatatgaac aggttacaac
     7921 cggcccggtc gcggcattac attacgatgg cacaaccacc tcgatgtata acgagtttgg
     7981 cgacagtact accacgctga ccgatccgtt atggcatgcc agcgtgagta gcttaggctg
     8041 gcgtgttgac tcccggcttg gcgatctccg accctgggcg caaatcagct ataaccagca
     8101 atttggcgag aatatctgga aggcgcaatc aggcctgagc cggatgacgg cgacaaacca
     8161 gaacggcaac tggctggatg tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc
     8221 cgcctatgct gcgctaactc aggcagaaaa taccactaat aatagcgact atctgtatac
     8281 gatgggggtt agcgccagat tttaacgtaa cagtcacaat tgaaaccatt gaataacaac
     8341 agttgtggcg atagtgggtg ctaacttacc aaataataaa tttggtgaat aattgtcgcg
     8401 tcattcattc ctgaactaag gcatttcatt ccgttctgat ggcatttcat gccgtttttc
     8461 cccaagcata aaatgcactt cgttatggtt gtcggcagag atttttcctt tttattactg
     8521 caggaatact gccatgacac cttcaaatta tcagcgtacc cgctggctga cactcatcgg
     8581 tactatcatt acccagtttg cgctggggtc ggtttatacc cggagcctgt ttaatggcgc
     8641 gctttctgcc aagctggatg cgccggtaag ccaggtcgct ttctctttcg gcttgttaag
     8701 tctggggctg gcaatttcgt cttctgttgc gggcaaattg caggaacgtt ttggcgttaa
     8761 acgcgtcacc gtggcttccg gcattttgct gggattaggc ttcttcctga ccgcgcattc
     8821 caacaacctg atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc
     8881 gggttacctg ctgacgctct ctaactgcgt gaagtggttc ccggagcgta aaggtctgat
     8941 ctccgcgttc gctatcggtt cttatggtct gggtagcctg ggtttcaaat ttatcgacac
     9001 gcagctgctc gaaacggtgg gtctggaaaa aacctttgtg atttggggag cgattgcgct
     9061 ggtgatgatt gttttcggcg caacgttaat gaaagacgca ccaaaacagg aagtgaaaac
     9121 cagcaatggt gtggtggaga aagattacac gctggcagag tcgatgcgta aaccgcagta
     9181 ctggatgtta gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg tgattggggt
     9241 agcgaaagat atcgcccaaa gtctggcaca ccttgatgtg gtttccgcag ccaatgcagt
     9301 aacggttatt tccatcgcta acctttcagg tcgtctggtg ctgggcattc tgtctgacaa
     9361 aattgcccgt atccgtgtta tcaccattgg tcaggtgatt tcgctggtgg gtatggcggc
     9421 cctgctgttt gcaccattaa atgcagtgac gttctttgca gcgattgcct gcgtggcatt
     9481 taactttggc ggcactatta ccgtctttcc atcactggtc agtgagttct tcggcctcaa
     9541 taacctggcg aaaaactacg gtgtgattta cctcggtttc ggtatcggta gcatttttgg
     9601 ttcgattatc gcctcactgt ttggcggttt ctatgtgacc ttctacgtga ttttcgccct
     9661 gctgattctg tcattggcgc tttctacgac gattcgtcag ccagagcaga aaatgttgcg
     9721 tgaggcgcat ggctcccttt aatgctcagc cgcgagctac tgggcttcag cttccgtgtg
     9781 catcaaacgc atcaacgaaa tgtataaaca atgactcgta aaatttgggc cagttaactg
     9841 gcccttttta ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat
     9901 ttgtgcttca tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg
     9961 cgcgtgtaga ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag
    10021 ccgctaagca gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa
    10081 tcgattacac agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat
    10141 ggcgcggttt tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc
    10201 gtttctgctg ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt
    10261 ctatcgctgt ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc
    10321 gcaggtgcca gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct
    10381 tccgtcatga ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc
    10441 tggttaatcg ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac
    10501 accttgctcc gacaactgcg aaccccaggg cagcgtattc gcagcctggt ggtcgtcgta
    10561 ctggcgggta ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg
    10621 gagagggcga ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg
    10681 ccatcaaact ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc
    10741 gataataatt cattgcttaa tccggcgaag aagtttacat accaggctcc acaaaacgtt
    10801 gatgatactt atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggtatt
    10861 ttcggctatg agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc
    10921 cgtggttact cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag
    10981 gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggttctg
    11041 aagcagttag gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc
    11101 aacacgatgg cagacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt
    11161 ggcaagccgg tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac
    11221 ccggatggta agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag
    11281 cgttatccgc gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt
    11341 accaaagcgc agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc
    11401 tccagcgtga ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac
    11461 ggtgagtcaa ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg
    11521 gagcagttcc gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc
    11581 aatgcgcagg cgtttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcac
    11641 gtagagctgt acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt
    11701 aacgaaaaca acaactggtg tcacatcccg cagacaaaag tggcagcggc taactaagcg
    11761 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
    11821 atctgcgcag taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg
    11881 ttcgggacga aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg
    11941 ctcaacgagc aggttttttt gcatttgtag ctcatgagga tgagaacctc cgggggcagg
    12001 gaggctcgac tcgacgtagg cctgataaga cgcgccagcg tcgcatcagg caacggcacc
    12061 gcacttattg tcggatgcgg cgcgaccgcc ttattcgacc aacacaccac acattttcct
    12121 gccaactttg cgccccatat ctcactatcc attaacggat ttgtgacaga aaccattgtc
    12181 tttttttata tttgcttaaa tcttttttgc gttacttatg gctggcgctg gcattttccg
    12241 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
    12301 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
    12361 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
    12421 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
    12481 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
    12541 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
    12601 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
    12661 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
    12721 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
    12781 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
    12841 tcccgcttta taaccgcctg gttgaattta aaatcggcac caccgaagtg atcccgggcc
    12901 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
    12961 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg cgaaatgaac gccgatgacg
    13021 tggtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc gtaccataaa gtttctggcg
    13081 gcagctacga atacttcgaa ggtatgggct tgccggagct gatcagcgaa gtgaaaaagg
    13141 tggacgataa caccgttcag tttgtgctga ctcgaccgga agcgccgttc ctcgctgacc
    13201 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgacgcgatg atgaaagccg
    13261 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
    13321 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
    13381 agatcgatac gctggtcttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
    13441 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatagct cgcatgaagc
    13501 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcgtata
    13561 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca agcactaacc tacgcggtga
    13621 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
    13681 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
    13741 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
    13801 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
    13861 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    13921 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    13981 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    14041 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    14101 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcagg
    14161 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    14221 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    14281 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    14341 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    14401 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    14461 cctctgcgtg cagcgccatc cggcagcaat acttcattct ctgtccgaca agggcgggaa
    14521 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg agtcactata gagaatccgg
    14581 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    14641 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    14701 ggcgaacgtg ggatctcccc tgagcgtcac gtgcagctgc tggctgaact cggcttagat
    14761 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    14821 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    14881 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    14941 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    15001 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    15061 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    15121 ccgttaaccg gttttatgct aatcgacacc gccatctggg gtgaagacgg caactttatc
    15181 gatgccgtcg cccatatgat cttgcccgcc attgtgctgg gtactattcc gctagcggtc
    15241 attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg gcgaggatta catccgcacc
    15301 gcgcgcgcca aagggctaac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    15361 atgctgccgg tagtgaccgt tatcggcctc caggtgggaa cattgctggc gggggcgatt
    15421 ctgaccgaaa ccatcttctc gtggcccggt ctggggcgct ggttgattga cgcactgcaa
    15481 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    15541 gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    15601 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    15661 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg
    15721 gtttacgtcg ccatcgtgct gttcatcgcg atctttgcca actggattgc gccttataac
    15781 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagt
    15841 atggcacact tgctgggcac cgatgacgtc ggtcgtgatg tgctgtcgcg gctgatgtac
    15901 ggcgcgcgtt tatcgctgct ggttggctgt ctggtggtgg tgttgtcgct gattatgggc
    15961 gttattctcg gcctgatcgc cggttacttc ggcggcctgg ttgataacat tatcatgcgc
    16021 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    16081 ttcggcccgt cgattggtaa cgccgcgctg gcgctgacct tcgttgcctt gccgcactat
    16141 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt caccgcctct
    16201 cgcgtggcag gtgccggggc gatgcgccag atgtttatta acatcttccc gaactgcctt
    16261 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct
    16321 cttggtttcc tcggcatggg ggcacagccg ccaacgcctg agtggggcac catgctctcc
    16381 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttccctgg tctggcgatc
    16441 ctgttgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    16501 aaactaaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    16561 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    16621 gggcgaagtg gtcgggattg tgggtgagtc cggctccggt aagtcggtca gttcactggc
    16681 gattatgggg ctgattgatt atccgggccg cgtaatggca gaaaaactgg agtttaacgg
    16741 ccaggatttg cagcgtatct cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    16801 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    16861 tatggaagcg attaaagtgc atcagggcgg caacaaaagt acccgccgtc agcgggcgat
    16921 tgacctgctg aatcaggtcg gtattcccga tccggcttcg cgtctggatg tttacccgca
    16981 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    17041 aaaactgctg attgccgatg aaccgaccac cgcgctggac gtgaccattc aggcgcaaat
    17101 catcgaacta ctgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    17161 tgacctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggtcaggt
    17221 agtagaaacg ggcgatgcac acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    17281 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    17341 tgttgttccc ggcaagtatg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    17401 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg atgggcgtca
    17461 gtccaaatgc cattacccac ttgatgatgc cgggaggccc acactatgag tacgcaagag
    17521 accacctcgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    17581 aaaggcatgt tcgtgccgga acgtctggtg aaggcgctgg acggcgtttc gtttaacctt
    17641 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    17701 cggttgctga cgatgatcga aacgcccacc ggcggcgagc tgtattatca ggggcaggat
    17761 ctgctcaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    17821 cagaatccct acggttcgct gaatccgcgt aagaaagttg ggcaaattct cgaagagccg
    17881 ctgcttatta acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    17941 gcaaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcacatgtt ttccggcgga
    18001 caacgtcagc gtatcgctat tgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    18061 gatgagccgg tttctgcgct ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg
    18121 gatttgcagc aggagttggg gctttcttat gtctttatct cccacgacct gtcggttgtg
    18181 gagcacattg ctgatgaagt gatggtgatg tacctaggcc gctgcgtgga gaaaggaacg
    18241 aaggaccaaa tcttcaataa cccgcgccat ccgtacaccc aggcgctgct ctccgcgacg
    18301 ccacgcctga acccggacga tcgtcgtgaa cgcatcaaac tcaccggtga actgccaagc
    18361 ccgctgaatc ctccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    18421 tgcacccagt tgcagccgca gctaaaagac tacggaggtc aactggtagc ttgttttgct
    18481 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    18541 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtaaag cagaatcagc
    18601 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    18661 atgaggcacg acacaatgcc atataacggg cttcccaact gaaagaacac cagcactgat
    18721 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    18781 agcatgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    18841 cctttaatag cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    18901 atattcagca ccgtagaggt gatatgaatg atatgtccag ggatcacctg cgcggccagt
    18961 gccagtgccg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    19021 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    19081 gtacgcagcg cgagccgggt tgccagtact ttatctgctt cccgtttacg ataggcaata
    19141 ttcattgggt taagtacctg aataaatacc gcagaaaaga agcaaaatgg aatggtaagc
    19201 aggacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    19261 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    19321 ccagaaatct taaacagtaa tcgttcacca ccagacgcaa tcgctatcag tacggcgaaa
    19381 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    19441 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    19501 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    19561 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    19621 actacccagg tgacaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    19681 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    19741 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgctt cgatagtgtg
    19801 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    19861 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    19921 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    19981 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    20041 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agca
//
LOCUS       DAZSNM010000062        19939 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZSNM010000062 DAZSNM010000000
VERSION     DAZSNM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19939
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.062"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..827)
                     /locus_tag="ACHW42_001841"
     CDS             complement(<1..827)
                     /locus_tag="ACHW42_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR8943683.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     "
     gene            complement(865..2145)
                     /gene="puuB"
                     /locus_tag="ACHW42_001842"
     CDS             complement(865..2145)
                     /gene="puuB"
                     /locus_tag="ACHW42_001842"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR8943684.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(2147..3634)
                     /gene="puuC"
                     /locus_tag="ACHW42_001843"
     CDS             complement(2147..3634)
                     /gene="puuC"
                     /locus_tag="ACHW42_001843"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR8943685.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(3784..4341)
                     /gene="puuR"
                     /locus_tag="ACHW42_001844"
     CDS             complement(3784..4341)
                     /gene="puuR"
                     /locus_tag="ACHW42_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR8943686.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(4368..5132)
                     /gene="puuD"
                     /locus_tag="ACHW42_001845"
     CDS             complement(4368..5132)
                     /gene="puuD"
                     /locus_tag="ACHW42_001845"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR8943687.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            5344..6762
                     /gene="puuA"
                     /locus_tag="ACHW42_001846"
     CDS             5344..6762
                     /gene="puuA"
                     /locus_tag="ACHW42_001846"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR8943688.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            6911..7075
                     /locus_tag="ACHW42_001847"
     CDS             6911..7075
                     /locus_tag="ACHW42_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8943689.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            7065..8450
                     /gene="puuP"
                     /locus_tag="ACHW42_001848"
     CDS             7065..8450
                     /gene="puuP"
                     /locus_tag="ACHW42_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR8943690.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            8584..8829
                     /locus_tag="ACHW42_001849"
     CDS             8584..8829
                     /locus_tag="ACHW42_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR8943691.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            9142..10785
                     /gene="sapA"
                     /locus_tag="ACHW42_001850"
     CDS             9142..10785
                     /gene="sapA"
                     /locus_tag="ACHW42_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR8943692.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            10782..11747
                     /gene="sapB"
                     /locus_tag="ACHW42_001851"
     CDS             10782..11747
                     /gene="sapB"
                     /locus_tag="ACHW42_001851"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR8943693.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            11734..12624
                     /gene="sapC"
                     /locus_tag="ACHW42_001852"
     CDS             11734..12624
                     /gene="sapC"
                     /locus_tag="ACHW42_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR8943694.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            12624..13616
                     /gene="sapD"
                     /locus_tag="ACHW42_001853"
     CDS             12624..13616
                     /gene="sapD"
                     /locus_tag="ACHW42_001853"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR8943695.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            13618..14424
                     /gene="sapF"
                     /locus_tag="ACHW42_001854"
     CDS             13618..14424
                     /gene="sapF"
                     /locus_tag="ACHW42_001854"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR8943696.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            14492..14845
                     /locus_tag="ACHW42_001855"
     CDS             14492..14845
                     /locus_tag="ACHW42_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR8943697.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            15213..16001
                     /gene="fabI"
                     /locus_tag="ACHW42_001856"
     CDS             15213..16001
                     /gene="fabI"
                     /locus_tag="ACHW42_001856"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR8943698.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            16145..17272
                     /locus_tag="ACHW42_001857"
     CDS             16145..17272
                     /locus_tag="ACHW42_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFR8943699.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            17340..19274
                     /gene="rnb"
                     /locus_tag="ACHW42_001858"
     CDS             17340..19274
                     /gene="rnb"
                     /locus_tag="ACHW42_001858"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR8943700.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(19352..19455)
                     /locus_tag="ACHW42_001859"
                     /pseudo
     CDS             complement(19352..19455)
                     /locus_tag="ACHW42_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19509..>19939
                     /locus_tag="ACHW42_001860"
     CDS             19509..>19939
                     /locus_tag="ACHW42_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="c-di-GMP phosphodiesterase; probably degrades
                     signalling molecule c-di-GMP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR8943701.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDALRFSTTKTTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYT"
BASE COUNT         4473 a         5271 c         5263 g         4932 t
ORIGIN      
        1 gaaagcggca tcccgcccgc gaggcttttc gccatcgtca ttaagtccgg cttatcggcg
       61 taatgatcca tggcaaacag tttaccggta cgcgcaaagc cgctttgcac ttcatcggca
      121 atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg cggcaaccag ctcttttggc
      181 gcaacgttga aaccgcactc gccctgcacc ggttcgaaaa taatggccgc gacctgcttc
      241 gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga gggagtcctg tgttgaaatg
      301 ccgtgtaaat ctgacggata aggtacgtga tacaccgaac cagggaacgg gccgaagccg
      361 attttgtacg gcgcaacttt tccggtcagc gccatggtca tatatgtacg accgtggaag
      421 ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg cgcgagcaat tttcaccgcg
      481 ttttccaccg cttccgcgcc ggtagtgaag aatgcggttt tagcctgccc gctcactggg
      541 gcaagggtgt taattttctc cgccagcgtg acgtagcttt cgtacggcac aatctgatac
      601 gccgtgtggg taaactgttg caattgctgc tccaccgccg cgaccagatc agggtggcga
      661 tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga tgtattcgtt gccctctacg
      721 tccttcagcg tagcgttttc tgccgactgg gcgaagaagt tacacatcac gccaaccccg
      781 cgtggggtag cagaaagacg acgctgatgg aattcattgt tgctcatgtt tcacaccttt
      841 tgactcttca ctgttaatcg gaaattaaac gcccagacga tcgcgcaggc tgtaataagc
      901 cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg ccggggaacg ggtaatgcgg
      961 cagattggcg aaggcatcga aacgctcggc gtcaccgcgc agcagttcag caatcaaacg
     1021 cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa ccctgcatgt aatagatgtt
     1081 ggtatcaagg cggccgaact gcggcatgtg cgacaaggtc agcaggaagt tgcccgtcca
     1141 gcggtaatca attttcacgc ccttcaactg agggaaggtt tttagtagtt tcggtaccac
     1201 aagacgctca acgtcatccg ggttgcgtgc accatacact acacccccgc cgtacagcag
     1261 gcggttgtcg gcggtaagac ggtagtaatc aagcagatag ttacagtctt ccacacagta
     1321 gtttttcggg atcagcgaac gggctaaatc ttccgacagc cgttcggtgg tgatcacctg
     1381 cgtgccgcac ggcatgctgc gtttcgctag ttccggctct actttatcgc ccagatacgc
     1441 attcccggcg acaatcacat acttcgccgt cacctgacca ttggcagtcc gaaccacggc
     1501 tggcgtggtg tgctggatct gcgtcacggc ggaaagctca tacacgcgcc cgccgttcag
     1561 gcggatggca tcggcttcac caatggcgag gtttagcggg tgaatatgcc caccgctgtg
     1621 atccagcagc gcaccagtgt agcgatcgct ggcgacctcg cggcgaatag cgttgacgtc
     1681 cagcagctcc agctgtttat tgccgtagcg tacccagttc tctttctgct cttcgagtgt
     1741 ggcgagttgt ttatcattca tcgccacaaa cagaccgccg gggcggtagt cgcagtcaat
     1801 ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc tcgaacatca tgctgccgag
     1861 catacgggcg gtgtccatgc cgtagctttt ttcgatcacg tcgatgtcgc ggctgtagga
     1921 gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag ccgatgcgtg aggcttcgag
     1981 aaccactacg tcaaagcccg cttccgccag atgcagcgcg gaggagagcc cggtatagcc
     2041 gccgccaacc acgcaaacgt cgcaggtgat tgactcattc agtgtgtcga atggtgcata
     2101 tttattcgca ctggcggcgt agtaactgct ggtatgttcg gtcatttcag gcctccaggc
     2161 ttatccagat ggttttcagt tcagtgaatt tttcaagggc atgcagggat ttgtcgcgac
     2221 cgttgccgct ctgcttatag ccgccaaacg gcacggtcat atcgccgtcg ttgtagttat
     2281 tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg gtgcgcgcgg gagaggtcgc
     2341 gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt ggcaagctgc agcgcctgat
     2401 cttctgatgt gaaacgggtg accaccagca ccggaccgaa aatctcttcg cggcttaagg
     2461 acgcattcgg gtccacatcc acgaagatgg tcgggccgat ggccgctgcc agcccggcgt
     2521 tgcgaccatc cagcagcagt tgccccttgc cttcgccttc acgaataaag gtgtgtaccg
     2581 agtcagcgtg agcgctgtcg attaaggtgc ccatggtggt tgcgggatca agtggatggc
     2641 ccggctgcca gttttgcgcc tgctgtttta acagggctaa gaattcatcg gcgatgctct
     2701 cttccagtaa caaacgcgtt ccggcgatgc gcacctggcc ctggttgtag aagatgcctg
     2761 cggcggtggc gcttgccgct ttttgcagat ccggacaatc ggcgaaaacg atgttggcgc
     2821 ttttgccgcc cgcttccagc cagacacgtt tcatgttgct atcacccgcg tctttcagca
     2881 gttgtttacc cgtacgcgtg gagccggtaa aggcgatggc atcgatatcg ttatgacgcg
     2941 aaagcgcctg ccccgcttca tgaccaaaac ccgtcaccac gttcaacaca ccatccggca
     3001 agcctgcttc tttcgccagc cccgcgagac gaatcgcact gagcggtgat ttttcagacg
     3061 gttttagaat cacgctgttt cccgccgcca gcgccgggcc gagtttccag caagtcagca
     3121 acagcgggaa gttccacggc acgatggcgg caatcacgcc gaccggttca cgcacgatca
     3181 tcgccagctc atggctactg gtggtcgcca cttcgccata cactttgtcg atcgcttcgg
     3241 cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc acgcagactg tgacgaatcg
     3301 gtttgccggt gtcgagagtt tccagcagtg ccagctcttc ggcgtgggct tccattaaat
     3361 cggcgagttt attcagtacc gctttacgtt tagccggaga agagagtgac cagtcgccgc
     3421 gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat atcgacgctc ttgccgcggg
     3481 caattttcgc cagcggtgcc tgggtgaccg gatcaacggt ttcaaaggtt tcattttccg
     3541 ccgcagcagt atattcaccg ttaataaata agcggttttc aatggcgaga cttaacgctt
     3601 tatcctgcca gtaagccaga tgatgaaaat tcattatgac tcctgtttca cgtctatcag
     3661 atatatgcag tttgtgaacg tcagggcgca gagatccccg ggctgcatgc ccgtttattt
     3721 ctggcgacat tctttatgga ttgccgggcg cattgcgctt acccggctta cagaacaaaa
     3781 agattaaaac gtggtgggcg tatgggcgct gataattcgg caaatacctg ccgacgtatt
     3841 actgaaactg tgcgggatgc cggtattaat ggcataactt tgccccgcga cgaggtggta
     3901 atcctgacca ttaatcgtca gaacaatttc accttccagt actgtgccta tttcctcacc
     3961 ctgatgctta attctttccc cagtggttgt gcccggctgg tacgtttcaa agatcatcgc
     4021 cagcgtgcga ttcgggttac cgttatgaac cagcttcatt gacacaccct gactgcccat
     4081 ctcaattaag tcgtcctgat taatgacgac ctgcggctca tcaggttttt ccggctcgga
     4141 aaagaattcc gagagtgaca gaccatacac cttcagcagc ttttgcagcg tactgatggc
     4201 agggctgact ttatcttgtt ctatcgtact gatagcactg tgagtcagcc cggagagttc
     4261 ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt tccgacaagc gttttcctgg
     4321 cgccagtccc tcatcactca tatttgcatt tccttaaact gtagtggtca gagtcgctgt
     4381 ttttcagcga tatggtgctg ccaagcggtg ataaagccct cgaacaatat acgcgaaagc
     4441 gcgtactcgc tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa aggatgattg
     4501 atgacgctaa ccgcctcaac taagccatcc ggcgagcgag cttcaacacg caaccgtggg
     4561 ctaacgacct tcgccccttg tccatgtaga gagtttaccc aaaagttgct acattcaggt
     4621 aacaacgcag acagtaatcc cccctcttca acctgaactt catgcgacgg tgcatattgc
     4681 tgttccaccg gcagttcggg atcttcccga tgttccagca attcaggctg ttcgcacagc
     4741 ttgcgatgca acgaaccacc ggttgccacc accagttctt gtaaaccccg gcagatggcg
     4801 aaaatgggga tgcgcctttc gagtgcggca ttaattatcg ccatgctcag aagatcacgc
     4861 ccgggatcgg cgtcaggctc atcgccgttt tcaccatata ggtgcggctg cacattgctg
     4921 ggactactag gaagataaat gccatcgagt ttcggcaaaa gttgttcaag taacgacggt
     4981 tccgccagcg catgtggtag cgcaattggc aagccgcctg cgtggatgat ggcattcagg
     5041 tacttttctt gcagagtctg ggtcgcatga cccttaagcc tgttcctgca cattacgaca
     5101 ccgataaccg gattgttcat tatattttcc atgctcaatc tcacaaaatg gactaaatta
     5161 tcgccattac tgcgctttaa atgaccttta tgttcaatat tttttcaatc tagcagtgga
     5221 ttttcatttt tgcaaactca atttaacatt tgacaaacat ttggtttgca tacagattcg
     5281 aatgatggtc attatatttt acgctttgat aacgagcgga aaacaaacca aaggcgaaga
     5341 atcatggaaa ccaatatcgt tgaagtagag aactttgttc agcagtcaga agagaggcgg
     5401 ggtagcgcct ttacgcagga agtgaaacgc tacctggagc gctacccgaa tacgcaatat
     5461 gttgatgttt tacttactga tttgaatggt tgcttccgtg gaaaacgtat ccctgtttca
     5521 agcctgaaga agctcgagaa agggtgttat ttcccggcct cggtatttgc aatggatatt
     5581 ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa tgggcgagcc ggatcgtacc
     5641 tgtgttcctg ttctcggttc cttaactcct tctgccgccg atccagagtt tatcggtcag
     5701 atgctcctga ccatggtcga tgaagatggc gctccctttg acgttgagcc gcggaacgtt
     5761 cttaaccgcc tgtggcagca gctgcgccag cgcggattgt tcccggttgt agcggtagag
     5821 ctggagttct atttactgga tcgccagcgc gacgctgaag gggatctgca accgccctgc
     5881 gcgcctggca ccgatgaccg caatacacaa agccaggttt actccgttga taacctcaac
     5941 cacttcgccg acgtgctcaa tgatattgat gaactggcgc agttgcagct gattccggca
     6001 gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa tcaaccttta ccatactgat
     6061 aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac gcctagtgcg tctgatggca
     6121 gaaaagcata agatgcacgc cacttttatg gcgaagccgt atgaagagca cgcgggcagc
     6181 gggatgcata tccatatcag tatgcaaaat aatcgtggcg agaatgtgct ttctgacaca
     6241 gaaggcgaag attcgccgct gctgaaaaag atgctcgccg ggatgattga cctgatgccg
     6301 tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc gccgcttcca gccgggaatg
     6361 tatgtgccga cgcaggcgtc gtggggccat aacaaccgca ccgtcgccct gcgtattccg
     6421 tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg cgggtgccga tgccaaccca
     6481 tatctggtga tggcagcgat ttttgccggt attttgcatg gccttgataa cgagctgccg
     6541 ttgcaggaag aagtcgaagg caacgggctg gaacaggaag gcttaccctt cccgattcgc
     6601 cagagcgatg ccctgggtga gtttatcgag aatgatcacc tgcgccgcta tttaggcgaa
     6661 cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc tgttgcagtt tgagcgcctc
     6721 atcacagaaa cagaaattga gtggatgttg aaaaacgcgt aattcccctt gcggggttgt
     6781 ttcatcggcc tgagcggagc caggcctgac atttgctggt aatccagaca tttcccggat
     6841 gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa cgattaagac tgtgacgagg
     6901 aaatgagatg atgccgatga ttaaatcccc gcatggcgag ggaggctgcg tatgcgcccc
     6961 tcctgctact gactggactc cgcccccttt gcttcccttg ctaaacaggt ttgatttcag
     7021 gtcgacacga ccgcaaacct tattacgcag gggaggcagc aattatggct attaattcac
     7081 cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg aaaatcactg aaattgtggc
     7141 aggtggtaat gatgggtctg gcctatctca cgccgatgac cgtatttgat acttttggta
     7201 ttgtctccgg cattagcgac ggtcacgttc cggcgtccta tttgctggcg ctggcgggcg
     7261 tgctgtttac cgctatcagc tacggcaaac tggttcgcca gtttccggag gccggttcgg
     7321 cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg atttatggtc ggctggtcat
     7381 cactgctgga ttatctcttt ttgccaatga tcaacgtttt gttggcgaaa atctatctct
     7441 ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt aaccttcgtc gccattttaa
     7501 ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt caataccctg tttgtactgg
     7561 tgcaaatctc catcatggtg gtgtttatct tcctggtggt tcagggactg cataaaggag
     7621 aaggcgttgg caccgtctgg tcacttcagc cgtttatcag cgagaacgcg cacctgatcc
     7681 cgattattac cggtgcgacg attgtctgtt tctcgttcct cggtttcgat gcggtgacca
     7741 cgctttcgga agagacgccg gacgccgcac gcgtgatccc gaaagccatc ttcctgacgg
     7801 cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt tatgcagctg ttctttcccg
     7861 atatcagccg ctttaaagac ccggacgccg cactgcctga aattgcgctg tacgtcggcg
     7921 gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt gaacacatta gcgtctggcc
     7981 tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat ggggcgtgac aatgtgtttc
     8041 cggagcgcgt gtttggctat gtgcacccaa aatggcggac tccggcactc aacgtcatta
     8101 tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt agtcaccgcg acagcactga
     8161 ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct gtcggtgttt aatcatttct
     8221 ggcggcgtaa aggaatgaat aaaagctgga aggatcactt ccactatttg ctgatgccgc
     8281 tggttggcgc gctgacggtg ggtgtgctgt gggttaacct agagtcaacg tcactgacac
     8341 tcggtctggt atgggcttcg ttaggcggcg catatttgtg gtatctgatc cgccgctatc
     8401 gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag cgaaacgtaa tagccggatg
     8461 cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca ttaactataa aaagatgtat
     8521 gacccggatc acgagtgcta tcatccgccc ttatgcttag cgcattaacc ccaggggcaa
     8581 ataatgaacc acgatatccc gctaaaatat tttgatattg ccgatgagta cgcgaccgaa
     8641 tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg cccactactt ccagttactg
     8701 ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag cccaacatga aatggctgct
     8761 gaagcgggga ttaatcccgt acgcattgat gagatcgcag agtttttgaa tcaatggggt
     8821 aatgagtaat tatttaattg cagacgatcg gtcagtatcg ttgtccggat aaagcgttca
     8881 cgccgcatcc ggctaacaat gacacgcact gcagcgtgct ttaatgggtt tctcacgatc
     8941 gtaccgctct gctcctcgct aagttgttct gtgagttata gttatagaga aatcacgggt
     9001 acgaatgttg aacagatggc gacgccacct gttcaacgac caaccacacg gccctcatta
     9061 cgacccctga acacaaacgg taatggcaaa tgtcattaaa tattagtaag atagcctctt
     9121 tacataaaat cccttaatat tatgcgccag gtattatcgt ctcttttggt gattgctgga
     9181 cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc cgcatgctga tatccgcgac
     9241 agcggttttg tctattgcgt cagcgggcaa gtcaacacct ttaacccatc caaagcgagc
     9301 agtgggttaa ttgtcgatac ccttgccgcc cagttttatg atcgactgct ggatgtcgat
     9361 ccctatacct atcgcctgat gccggaactt gccgaaagct gggaagtact cgacaacggc
     9421 gcgacctatc gcttccacct gcgtcgcgat gttccgtttc aaaaaaccga ctggtttact
     9481 cccactcgta aaatgaatgc cgacgatgtg gtgtttacct tccagcgaat ttttgaccgc
     9541 aacaacccgt ggcataacgt caacggcagc aacttcccct acttcgacag cctgcaattt
     9601 gccgataacg tgaaaagcgt ccgcaaactg gataatcata ccgttgagtt ccgactcgcc
     9661 cagccggatg cttctttttt gtggcacctc gcaacccatt atgcttcggt catgtcggca
     9721 gaatatgccc ggaagttaga gaaagaagat cgccaggagc aactcgaccg tcaaccggtc
     9781 ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc aatttattcg cctacaacgt
     9841 catgatgact tctggcgcgg taaaccgtta atgccgcagg tagtggtgga tttaggctcc
     9901 ggcggcaccg gacgtctgtc gaaactcctg accggggaat gcgacgttct ggcctggcct
     9961 gctgccagcc agctatccat tttgcgtgac gacccgcgct tgcgtttaac gctgcgtcct
    10021 gggatgaacg tcgcctatct ggcatttaac accgccaaac cgccgctaaa taatcccgct
    10081 gtccgccatg cactggcact ggcgattaat aaccagcgcc tgatgcaatc catctattat
    10141 ggtacggctg aaacggcggc ctctatttta ccgcgcgcct cgtgggccta tgacaacgag
    10201 gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac agttgaaggc gttggggctg
    10261 gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc aggcgtggaa ccccagtcca
    10321 ctgaaaactg ccgaactgat tcaggcggat atggcgcagg ttggcgtaaa agtggtgatt
    10381 gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg atatgagcca tgatctgacg
    10441 ttatccggtt gggcgacgga cagtaacgac ccggacagtt tcttccgtcc gttactgagc
    10501 tgcgcggcaa ttcattcaca gaccaacctc gcccactggt gcaatccgaa attcgacagc
    10561 gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc gtattgaagc ctatgacgaa
    10621 gcgcagagta ttctggcgca ggaattacct attctgccgc tggcgtcgtc attgcgtttg
    10681 caggcctatc ggtacgatat caaaggtctg gtacttagcc cgtttggtaa cgcctccttt
    10741 gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac catgattatc ttcaccttac
    10801 gccgcatttt gttattgatt gtcaccttgt tcctgctgac cttcgttggc ttcagcttga
    10861 gctatttcac tccacacgcg ccgttacaag gcgcgtcact gtggaatgcc tgggtgttct
    10921 ggtttaatgg cctgatccac tgggattttg gtgtttccag cattaacggt caaccaattg
    10981 ccgaacagtt aaaagagctc ttcccggcga cgatagaact gtgcatcctc gccttcggtt
    11041 ttgcgctgat tgttggcatc cccgtgggga tgattgccgg gattacgcgc cataagtggc
    11101 aggataactt gatcaacgcc atcgccttac tgggcttttc aatcccggtg ttctggctgg
    11161 cgcttctgtt gacactgttt tgttcactca cgctaggttg gttgcccgtt tcagggcgtt
    11221 tcgatctgct ctacgaagtg aaaccgatta ccggttttgc gttgattgat gcctggcttt
    11281 cggactcacc atggcgggat gaaatgatca tgagcgcaat ccgccatatg atattgcccg
    11341 tgatcaccct gtcggttgcg ccaacaactg aagtgatccg cctgatgcgt atcagcacta
    11401 ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac ccgcggtttg tcgcgcttta
    11461 ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc ggttattcct cgcctgggct
    11521 tacagttttc caccatgctg acgctggcga tgatcaccga aatggttttt agctggccgg
    11581 gtttagggcg ctggttaatt aacgccatcc gccagcagga ttacgcagcc atttccgccg
    11641 gagtgatggt gtgtggctca ctggtcatta ttgttaacgt gatttctgat attttgggtg
    11701 ccatggctaa ccctctgaaa cataaggaat ggtatgcctt acgatagcgt atacagcgaa
    11761 aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat tttatagtga cgcctctgcg
    11821 atggtcggcc tgtacggctg cgcgggactg gctgtactgt gtatttttgg cggctggttt
    11881 gcgccttacg gcatcgacca gcaatttctc ggttatcaat tattgccgcc atcatggtcg
    11941 cgctatggcg aagtttcttt cttcctgggg actgacgacc tcggacgtga tgttttaagc
    12001 cgtttactga gcggagctgc gccaaccgtg ggtggcgcat ttgtggtgac gcttgccgcg
    12061 acgatctgtg gcctggtact gggcaccttc gccggggcga cacacggcct ccgctcagcg
    12121 gtgcttaacc atattctgga taccttactg gcgatccctt cgctgctgtt ggcaattatc
    12181 gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt ttgctgtctg gctggcattg
    12241 ctgccgcgta tggtacgttc gatttacagc atggtgcatg acgaactgga aaaagagtac
    12301 gttatcgccg cccgtctgga tggcgcatca acgctgaata ttctctggtt tgctgtgatg
    12361 ccaaacatca ccgccgggct ggtcaccgag atcacccgcg cactgtcgat ggcaattctc
    12421 gatatcgccg cgctgggctt tctcgatctc ggcgcacaac tcccctcgcc tgaatgggga
    12481 gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc cgtggactgt catgctgcca
    12541 ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc ttggcgatgg cgttcgtcgt
    12601 gcgattattg cgggggtgga ataatgccat tactcgatat tcgtaacctg accattgaat
    12661 ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt aagtatgacc ttaaccgaag
    12721 gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa gagtttgatt gcgaaagcga
    12781 tttgtggggt gaataaagat aactggcgtg ttactgctga ccgtatgcgt tttgatgata
    12841 tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact ggttggccat aacgtgtcga
    12901 tgattttcca ggaaccgcag tcgtgtcttg acccttcaga acgtgtgggt cgccagttga
    12961 tgcaaaacat cccagcctgg acctataaag gccgttggtg gcagcgtttt ggctggcgca
    13021 aacgccgtgc cattgaactg ctgcaccgcg tggggattaa agatcacaaa gatgcgatgc
    13081 gcagttttcc ctatgagttg accgaaggtg aatgtcagaa agtgatgata gccattgcac
    13141 tggcgaatca accgcgtctg ctgattgctg acgaaccgac aaactcaatg gagccaacaa
    13201 cccaggcgca aatctttcgc ctgctgacgc gtctcaacca aaacagcaat accactattt
    13261 tgcttatcag ccatgactta caaatgctta gccaatgggc ggataaaatt aacgtgcttt
    13321 actgcggtca aacagtggaa accgcgccga gtaaggagtt ggtgacgatg ccacatcatc
    13381 cttataccca ggcgctgatc cgcgcgatac cagacttcgg cagcgcgatg ccgcataaaa
    13441 gtcgcctcaa tactctaccc ggcgctatcc cattgctgga acagttaccg attggttgtc
    13501 gtctggggcc acgttgcccg tatgcacaac gagaatgcat tgtgacgcca cgtttgacgg
    13561 gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa catggagaaa gagtgagatg
    13621 atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt tccgctaccg gaccggttgg
    13681 tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct ttacgctacg tgaaggccag
    13741 acactggcga ttattggcga gaatggttcg ggtaaatcca cactggcaaa aatgctggcg
    13801 ggaatgatag aacctaccag cggcgagtta ttgattgacg atcatccgct gcattttggc
    13861 gattattcct tccgtagtca gcgtattcgc atgatttttc aggatccttc gacctcgttg
    13921 aatccccgtc aacgtatctc acaaattctg gattttccac tgcgcctgaa caccgacctg
    13981 gaaccggaac agcgtcgcaa acagattatt gaaacgatgc gtatggttgg gctgctaccg
    14041 gatcacgtca gttactatcc gcatatgttg gcacccggac aaaaacagcg tctgggtctg
    14101 gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg atgaagccct cgcttcactg
    14161 gatatgtcaa tgcgttcgca gttgattaat ctgatgctgg agttacagga aaaacagggc
    14221 atttcgtata tttatgttac ccagcatatc ggaatgatga agcacattag cgaccaggtg
    14281 ctggtgatgc atcagggcga ggttgtcgag cgaggcagca ccgcggatgt gctggcatcg
    14341 ccgctgcatg aactcaccaa acggctgatt gccgggcact ttggcgaagc attgacagcg
    14401 gatgcgtggc gtaaagatcg ctaattttct gaatcctcct tccagattat tcacctcatt
    14461 ctgacaagag attaaatctt gtcagaatga ggtgatggat aaaataaaat caaatgcgcg
    14521 tgatttaaga cgcaatctca ctttgcagga acgaaagctc tggcgatatc ttcgcagccg
    14581 acgttttggt gatttcaaat ttcgccgtca acatccggtg gggagctaca ttctcgattt
    14641 tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt gggcagcatg atttggcagt
    14701 tgcctatgat accaggcgca ctagctggct tgagtcgcag ggctggaccg tgctgcgttt
    14761 ctggaataac gagattgatt gtaatgagga ggcggtgctg gagattattc tgcaggaact
    14821 gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc gaccgagccg aatagctgtt
    14881 gtggtgaaaa catggagaca gtgctggaga atattcggca aggtctgaac catcccagcc
    14941 atcgccataa aagggttagg ggctgtatga gcctgtttgt tgctggggta acaatatttg
    15001 cacaatatgg tcccctcgcc cctctgggga gagggttagg gtaaggggaa aagcgccccc
    15061 cctgccgcag cctgctccgg tcggacctgg taactatagc tactcacagc caggttgatt
    15121 ataataaccg tttatctgtt cgtactgttt actaaaacga cgaatcgcct gattttcagg
    15181 cacaacaagc atcaacaata aggattaaag ctatgggttt tctttccggt aagcgcattc
    15241 tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg tatcgctcag gcgatgcacc
    15301 gcgaaggagc tgaactggca ttcacctacc agaacgacaa actgaaaggc cgcgtagaag
    15361 aatttgccgc tcaattgggt tctgacatcg ttctgcagtg cgatgttgca gaagatgcca
    15421 gcatcgacac catgttcgct gaactgggga aagtttggcc gaaatttgac ggtttcgtac
    15481 actctattgg ttttgcacct ggcgatcagc tggatggtga ctatgttaac gccgttaccc
    15541 gtgaaggctt caaaattgcc cacgacatca gctcctacag cttcgttgca atggcaaaag
    15601 cttgccgctc catgctgaat ccgggttctg ccctgctgac cctttcctac cttggcgctg
    15661 agcgcgctat cccgaactac aacgttatgg gtctggcaaa agcgtctctg gaagcgaacg
    15721 tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg tgttaacgcc atctctgctg
    15781 gtccgatccg tactctggcg gcctccggta tcaaagactt ccgcaaaatg ctggctcatt
    15841 gcgaagccgt taccccgatt cgccgtacag ttactattga agatgtgggt aactctgcgg
    15901 cattcctgtg ctccgatctc tctgccggta tctccggtga agtagtccac gttgacggcg
    15961 gtttcagcat cgctgcaatg aacgaactcg aactgaaata atcgttctgt tggtaaagat
    16021 gggcggcgtt ctgccgcccg ttatctctgt tatacctttc tgatatttgt tatcgccgat
    16081 ccgtctttct ccccttcccg ccttgcgtca ggataacgat ttcctttacg accaaggagc
    16141 gcccatggaa caacgccaca tcaccggcaa aagccactgg tatcatgaaa cgcaatccag
    16201 tactacggag tatgacgttc tgcctctggt cccggaagcc gcaaaggtca gcgatccctt
    16261 tctgctcgac gtgatccttg atgaagagac gctggccccc ttcctttcat ggctggtccc
    16321 tgcgcgcgtt cttgcagtgg agttgttccc tgaccagctt accgtgaccc gttcacaaac
    16381 cttcaccgct tatgaacgct tgtcgacggc cctgacggtt gctcaggttt gcggcgtcca
    16441 gcggttatgt aactactatt cggcgcgact tacgccgctc cccggacctg attcctccag
    16501 ggaaagtaat catcggttgg cacaaatcac gcaatatgcc cgccaactgg ctagctcgcc
    16561 ttctattatc gacaaccgat cgcgccagca tctgaatgac gtcggtctta ctgtctggga
    16621 ctgtgtgatc attaaccaaa tcattggttt tattggcttt caggcgcgga caattgcgac
    16681 atttcaggct tatctcgggc atccggtacg ctggttaccc gggctggcga tacaaaacta
    16741 cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg cgaagcagct atgaggtgga
    16801 aaaactacct gaagagcaca caaaaagttc aactgcagaa ctttgccaac tggccgaaac
    16861 actctctctc caccctattt cactttccct tctcgaaaag ttgttaaaca gcacacgggt
    16921 caatgcacag ccggataatc agcttgcggc gttgttatgc gcgcgtataa atggcagtcc
    16981 tgcttgtttt tccacctgta tggattcatc aaatgaatat aaaaaaatca gcacccttct
    17041 gcgcaagggc gaaaatgaaa ttaaccgatg ggctgaccgt cattctgttg agcgcgctac
    17101 cgttcaggcg atacaatggc tgacccgagc acccgatcgc tttagcgccg cccagttcag
    17161 ccctttactc gaacacgaaa aatcatcaac gcagattatt aatctgctgg tatggagcgg
    17221 gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt gagacatatt aaccttgccg
    17281 cgtcagacag attcgcgtaa aactgtcagc cgctctaatg gccaccaaaa tagacaatta
    17341 tgtttcagga caacccgctg ctagcgcagc ttaaacagca actgcattcc cagacgccac
    17401 gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg cttcctggaa gtcgacgcac
    17461 aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt catgcatggc gaccgaatta
    17521 tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga gccagaagaa ctggttgaac
    17581 cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa tgaccgtctg accatcgttc
    17641 ctgatcatcc acttttaaaa gacgccattc cttgccgcgc agcccgtggc ctgaaccacg
    17701 agtttaaaga aggcgactgg gcggttgccg aaatgcgccg tcatccgctg aaaggcgatc
    17761 gttctttcta tgctgaactg acacaataca tcacttttgg tgacgaccac tttgtaccgt
    17821 ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc accagacggc gtcgctaccg
    17881 aaatgctcga tgaaggtctg gttcgtgaag atctgacctc gctggatttt gtcaccatcg
    17941 acagtgccag cactgaagat atggatgacg cccttttcgc taaggcgctg ccggatgaca
    18001 aacttcagct gattgtggcg attgccgatc caaccgcgtg gattgctgaa ggcagcaagc
    18061 tggacaaagc cgcgaaaatt cgcgcattca ccaactatct gcctggcttc aacatcccta
    18121 tgctgcctcg cgagctttct gacgatctct gctcactgcg cgccaatgaa gtccgcccgg
    18181 tactggcatg ccgcatgacg ctctccgctg atggcaccat tgaagataat atcgaattct
    18241 ttgccgccac catcgaatcc aaagcgaagc tggtgtatga ccaggtttct gactggctgg
    18301 aaaataccgg tgactggaag cctgaaagtg aagcaattgc cgaacaagtc cgtttgctag
    18361 cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca cgcactggtg tttaaagatc
    18421 gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt gctggatatc gtcgccgagc
    18481 ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat tgccgctaac atttgtgcgg
    18541 cccgcgtact gcgcgataag ctcggttttg gcatctataa cgtgcatatg ggctttgatc
    18601 cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca cggtctgcat gtcgatgccg
    18661 aagaagtgct cacgctggac ggtttctgca aactgcgtcg tgaactggat gcgcaaccaa
    18721 ctggtttcct cgacagccgc attcgtcgct tccagtcatt tgctgaaatt agctctgaac
    18781 ccggtcctca ctttggcctc ggtctggaag catacgccac ctggacttcg ccgatccgta
    18841 aatatggcga catgatcaac caccgtctgc tgaaagcggt tatcaaaggc gaaactgcga
    18901 cgcgtccgca ggatgaaatc actgtccaaa tggccgaacg tcgccgtctc aaccggatgg
    18961 cagaacgtga tgttggcgac tggttatacg cacgcttcct gaaagataaa gccgggaccg
    19021 gcacccgttt cgcggcggaa attgtcgata tcagccgtgg cggcatgcgt gtgcgtttgg
    19081 ttgataacgg cgctatcgcc tttattccgg cacctttctt acacgctgtg cgcgatgaac
    19141 tggtttgcag ccaggaaaac ggcactgtac aaattaaagg tgaaacggtt tacaaagtaa
    19201 ctgacgttat tgacgtcacc attgccgaag tccgcatgga aacccgcagc attattgcgc
    19261 gcccggtcgc gtaatctcct ttcacggccc attcctcatg gatgggccgt ttatttcccc
    19321 gctctacctt cgttatatcc aggcagattt tttaactgac tttcgtttga aaactggcgt
    19381 ttttccaaaa ctggattaca aataaatata taaaaaaaca atgcgtttag ccgccgttaa
    19441 tttcatgtaa gtcatgttgt tccttttatt taaccgcgga ctccgctgtt aaccggagga
    19501 tatgcatcat gaaaaccgtt agggagtcca caacgttgta caactttctc ggatcgcaca
    19561 atccatactg gcggttgacg gaaagcagcg atgctttgcg cttttctacc accaaaacca
    19621 cagaacctga tcgtacattg cagttatctg ccgaacaggc tgctcgcatc agggaaatga
    19681 cggtcatcac ctccagcctg atgatgagtc tgaccgtcga tgaaagcgat ctttctgtgc
    19741 atctggtagg acgaaaaatc aataaacggg aatgggctgg caacgcgtct gcatggcatg
    19801 acacaccggc ggttgctcgt gatttatcac acgggctttc ctttgctgag caggtagttt
    19861 ctgaagcaca ttccgcaata gtgattctcg acagccgggg gaatatccaa cgcttcaatc
    19921 ggttatgtga agattacac
//
LOCUS       DAZSNM010000063        19698 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZSNM010000063 DAZSNM010000000
VERSION     DAZSNM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.063"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHW42_001861"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHW42_001861"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR8943702.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHW42_001862"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHW42_001862"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR8943703.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHW42_001863"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHW42_001863"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR8943704.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="ACHW42_001864"
     CDS             6038..6658
                     /locus_tag="ACHW42_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR8943705.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="ACHW42_001865"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="ACHW42_001865"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR8943706.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHW42_001866"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHW42_001866"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR8943707.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHW42_001867"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHW42_001867"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR8943708.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="ACHW42_001868"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="ACHW42_001868"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR8943709.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10068..11069)
                     /locus_tag="ACHW42_001869"
     CDS             complement(10068..11069)
                     /locus_tag="ACHW42_001869"
                     /inference="COORDINATES: protein motif:HMM:NF007315.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8943710.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     ELLYRSMKFVSARNFWRHGASNGWASPMKAIRSCRSTRNHSGSFSRFLLNFVQIFVS"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHW42_001870"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHW42_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHW42_001871"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHW42_001871"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR8943711.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHW42_001872"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHW42_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR8943712.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHW42_001873"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHW42_001873"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR8943713.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHW42_001874"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHW42_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR8943714.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHW42_001875"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHW42_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR8943715.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHW42_001876"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHW42_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR8943716.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4683 a         5506 c         5031 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAZSNM010000064        19322 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZSNM010000064 DAZSNM010000000
VERSION     DAZSNM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19322
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.064"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..122
                     /locus_tag="ACHW42_001877"
     CDS             <1..122
                     /locus_tag="ACHW42_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR8943717.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            138..686
                     /gene="wcaF"
                     /locus_tag="ACHW42_001878"
     CDS             138..686
                     /gene="wcaF"
                     /locus_tag="ACHW42_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR8943718.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            712..1833
                     /gene="gmd"
                     /locus_tag="ACHW42_001879"
     CDS             712..1833
                     /gene="gmd"
                     /locus_tag="ACHW42_001879"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR8943719.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            1836..2804
                     /gene="fcl"
                     /locus_tag="ACHW42_001880"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             1836..2804
                     /gene="fcl"
                     /locus_tag="ACHW42_001880"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR8943720.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            2804..3283
                     /gene="gmm"
                     /locus_tag="ACHW42_001881"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             2804..3283
                     /gene="gmm"
                     /locus_tag="ACHW42_001881"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR8943721.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHW42_001882"
     CDS             3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHW42_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR8943722.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHW42_001883"
     CDS             4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHW42_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR8943723.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            6135..7505
                     /gene="cpsG"
                     /locus_tag="ACHW42_001884"
                     /gene_synonym="manB"
     CDS             6135..7505
                     /gene="cpsG"
                     /locus_tag="ACHW42_001884"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR8943724.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACHW42_001885"
     CDS             7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACHW42_001885"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFR8943725.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            8956..10434
                     /gene="wzxC"
                     /locus_tag="ACHW42_001886"
     CDS             8956..10434
                     /gene="wzxC"
                     /locus_tag="ACHW42_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFR8943726.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            10614..11894
                     /gene="wcaK"
                     /locus_tag="ACHW42_001887"
     CDS             10614..11894
                     /gene="wcaK"
                     /locus_tag="ACHW42_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFR8943727.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            11891..13111
                     /gene="wcaL"
                     /locus_tag="ACHW42_001888"
     CDS             11891..13111
                     /gene="wcaL"
                     /locus_tag="ACHW42_001888"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFR8943728.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            13122..14516
                     /gene="wcaM"
                     /locus_tag="ACHW42_001889"
     CDS             13122..14516
                     /gene="wcaM"
                     /locus_tag="ACHW42_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFR8943729.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            14691..15584
                     /gene="galF"
                     /locus_tag="ACHW42_001890"
     CDS             14691..15584
                     /gene="galF"
                     /locus_tag="ACHW42_001890"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFR8943730.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            15957..17042
                     /gene="rfbB"
                     /locus_tag="ACHW42_001891"
     CDS             15957..17042
                     /gene="rfbB"
                     /locus_tag="ACHW42_001891"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR8943731.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            17042..17941
                     /gene="rfbD"
                     /locus_tag="ACHW42_001892"
     CDS             17042..17941
                     /gene="rfbD"
                     /locus_tag="ACHW42_001892"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFR8943732.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            18000..18878
                     /gene="rfbA"
                     /locus_tag="ACHW42_001893"
     CDS             18000..18878
                     /gene="rfbA"
                     /locus_tag="ACHW42_001893"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR8943733.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            18883..>19322
                     /gene="rfbC"
                     /locus_tag="ACHW42_001894"
     CDS             18883..>19322
                     /gene="rfbC"
                     /locus_tag="ACHW42_001894"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFR8943734.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGI"
BASE COUNT         4802 a         4681 c         5269 g         4570 t
ORIGIN      
        1 gtgctgacgc caaaaaagtc caacggcaga tattacatgt acctggcttc tgggcagaat
       61 tatcctggca tttacgccaa cgtacgacag gtaagactaa agccttatat aacaaagtct
      121 gaatataagg aaaaccaatg caagatttaa gcggtttctc ggtgccgaaa gggttccggg
      181 gcggcaacgc tattaaagtg caattatggt gggcagtaca ggcaacaata tttgcctggt
      241 cgccacaagt attgtatcgc tggcgggctt ttttattacg tttattcgga gcaaaaatag
      301 gaaaaaacgt agttattcgt ccgtcagtaa aaattaccta tccgtggaaa ttaaccttag
      361 gtgattacgc gtgggttggc gatgacgtca atttatatac cctcggtgaa ataaccattg
      421 gcgcacattc ggtgatatcg caaaaaagtt atttatgcac cggtagccac gaccatgcaa
      481 gtcaacattt caccattaac gccacgccta ttgtgattgg cgagaaatgc tggctggcaa
      541 ccgatgtctt tgttgcccca ggcgtcacaa tcggcgacgg caccgtcgtg ggtgcacgaa
      601 gcagtgtttt taaatcgctt ccggcaaatg tggtttgccg ggggaatccc gcagtggtga
      661 tacgcgaacg cgttgaaact gaataaattc aaaaatacag aggaataaga catgtcaaaa
      721 gtcgctctca tcaccggtgt aaccggacaa gacggttctt acctggcaga gtttctgctg
      781 gaaaaaggtt acgaggtgca tggtattaag cgtcgcgcat cgtcattcaa caccgagcgc
      841 gtggatcaca tttatcagga tccgcacacc tgcaacccga aattccatct gcattatggc
      901 gacctgagtg atacctctaa cctgacgcgc attttgcgtg aagtacagcc ggatgaagtg
      961 tacaacctag gcgcaatgag ccacgttgcg gtctcttttg agtcaccaga atataccgct
     1021 gacgtcgacg cgatgggtac gctgcgcctg ctggaggcga tccgcttcct cggtctggaa
     1081 aagaaaactc gtttctatca ggcttccacc tctgaactgt atggtctggt gcaggaaatt
     1141 ccgcagaaag aaaccacgcc gttctacccg cgatctccgt atgcggtcgc caaactgtac
     1201 gcctactgga tcaccgttaa ctaccgcgaa tcctacggca tgtacgcttg taacggaatt
     1261 ctcttcaacc atgaatcccc gcgccgcggc gaaaccttcg ttacccgcaa aatcacccgc
     1321 gcaatcgcca acatcgccca ggggctggag tcgtgcctgt acctcggcaa tatggattcc
     1381 ctgcgtgact ggggccacgc caaagactac gtaaaaatgc agtggatgat gctgcaacag
     1441 gaacagccgg aagatttcgt tatcgcgacc ggcgttcagt actccgtacg tcagtttgtg
     1501 gaaatggcgg cagcacagct gggcatcaaa ctgcgctttg aaggcacggg tgttgaagag
     1561 aagggcattg tggtttccgt caccgggcat gacgcgccgg gcgttaaacc gggtgatgtg
     1621 attatcgccg ttgatccgcg ttacttccgt ccggcagaag ttgaaacgct gctcggcgac
     1681 ccgaccaaag cgcacgaaaa actgggctgg aaaccggaaa tcaccctcag agagatggtg
     1741 tctgaaatgg tggctaatga cctcgaagcg gcgaaaaaac actctctgct gaaatctcac
     1801 ggctacgacg tggcgatcgc gctggagtca taagcatgag taaacaacgc atttttatcg
     1861 ctggccattg tgggatggtc ggttctgcca tcaagcggca gctcgaacag cgcggtgatg
     1921 tggagctggt attacgtacc cgcgatgaac tgaacctgct ggacagccgc gccgtgcatg
     1981 atttctttgc cagcgaacgc attgaccagg tctatctggc ggcggcgaaa gtgggcggca
     2041 ttgttgccaa caacacctat ccggcggatt tcatctacca gaacatgatg attgagagca
     2101 acatcattca cgccgcgcat cagaacgacg tgaacaaact gatgtttctc ggatcgtcct
     2161 gcatctaccc gaaactggca aaacagccga tggcagaaag cgagttattg cagggcacgc
     2221 tggagccgac caacgagcct tacgccattg ccaaaatcgc cgggatcaaa ctgtgcgaat
     2281 cttacaaccg ccagtatgga cgcgattacc gcactgtcat gccgaccaac ctgtacgggc
     2341 cgcatgacaa cttccacccg agtaactccc atgtgatacc agcattgctg cgtcgcttcc
     2401 acgaggcgac ggcacagaat gcgccggacg tggtggtatg gggcagcggt acaccgatgc
     2461 gtgaattcct gcacgtcgat gatatggcgg cggcgagcat tcatgtcatg gagctggcgc
     2521 atgaagtctg gctggagaat acccaaccga tgctgtcgca cattaacgtc ggcacgggcg
     2581 ttgactgcac tatccgcgag ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggtc
     2641 gggtggtttt tgatgccagc aaaccggatg gtacgccgcg caaactgctg gatgtgacgc
     2701 gcctgcatca gcttggctgg tatcacgaaa tctcactgga agcggggctt gccagcactt
     2761 accagtggtt ccttgagaat caagaccgct ttcgggggga atgatgtttt tacgtcagga
     2821 agactttgcc actgtagtgc gctccacacc gcttgtctct ctcgacttta ttgtcgagaa
     2881 cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt
     2941 tgtgccggga gggcgcgtgc agaaagacga aacgctggaa gccgcatttg agcggctgac
     3001 gatggcggaa ctggggctgc gtctgccgat aacagcaggc cagttttacg gtgtctggca
     3061 gcatttttat gacgataact tctccggcac ggatttcacc actcactatg tggtgctcgg
     3121 ttttcgcttc agagtagcgg aagaagagct gttactgccg gatgagcagc atgacgatta
     3181 ccgctggctg acgccggacg cgctgctcgc cagtaacgat gttcacgcta acagccgagc
     3241 ctattttctc gctgagaagc gtgccggagt acccggatta tgaaaatact ggtctacggc
     3301 attaactact cgccggagtt aaccggcatc ggcaaataca ccggcgagat ggtggaatgg
     3361 ttggcggcac aaggtcatga ggtgcgggtc attaccgcac cgccttacta cccgcaatgg
     3421 caggtgggtg agaactattc cgcctggcgc tacaaacgag aagaaggggc cgccacggtg
     3481 tggcgctgcc cgctgtacgt gccaaaacag ccgagcaccc tgaaacgctt gttgcatctc
     3541 ggcagttttg ccgtcagcag tttctttcca ctgatggcgc aacgtcgctg gaagccggat
     3601 cgcattattg gcgtagtgcc aacgctgttt tgcacgccgg gaatgcgcct gctggcgaag
     3661 ctctctggtg cgcgtaccgt gctgcatatt caggattacg aagtggacgc catgctgggg
     3721 ctgggccttg ccggaaaagg caaaggcggc aaagtggcac agctggcaac ggcgttcgaa
     3781 cgtagcggac tgcataacgt cgataacgtt tccacgattt cgcgttcgat gatgaataaa
     3841 gccatcgaaa aaggcgtggc ggcggataac gtcatcttct tccccaactg gtcggaaatt
     3901 gcccgttttc agcatgttgc agacgccgat gttgatgccc ttcgtaacca gcttggcctg
     3961 ccggataaca aaaaaatcat tctttactcc ggcaatattg gtgaaaagca ggggctggaa
     4021 aacgttattg aagctgccga tctcctgcgc gatgaaccgc tgatttttgc cattgtcggg
     4081 cagggcggcg gcaaagcgcg gctggaaaaa atggcgcagc agcgtggact gcgcaacatg
     4141 caatttttcc cgctgcaatc gtatgacgct ttacctgcac tgctgaagat gggcgattgc
     4201 catctggtgg tgcaaaaacg cggcgcggca gatgccgtat tgccgtcgaa actgaccaat
     4261 attctggcgg taggcggtaa cgcggtgatt accgcagagg cccacacaga actggggcag
     4321 ctttgcgaaa cctttccggg tattgcggtt tgcgtagaac cggaatcggt cgaggcgctg
     4381 gtggcgggga ttcgtcaggc gctcctgctg cccaaacata acacggtggc acgtgaatat
     4441 gccgaacgca cgctcgataa agagaacgtg ttacgtcaat ttataaatga tattcgggga
     4501 taattatggc gcagtcgaaa ctctatccag ttgtgatggc aggtggctcc ggtagccgtt
     4561 tatggccgct ttcccgcgta ctttacccca agcagttttt atgcctgaaa ggcgatctca
     4621 ccatgctgca aaccaccatc tgccgcctga acggcgtgga gtgcgaaagc ccggtggtga
     4681 tttgcaatga gcagcaccgc tttattgtcg cggaacagct gcgtcaactg aacaaactca
     4741 ccgagaacat tattctcgaa ccggcagggc gtaacactgc acctgccatt gcactggcgg
     4801 cgctggcggc aaaacgtcat agcccggaga gcgacccgtt aatgctggta ttggcggcgg
     4861 atcatgtgat tgccgatgaa gacgcgttcc gtgccgccgt gcgtaatgcc atgccttatg
     4921 ccgaagcggg caagctggtg acctttggca ttgtgccgga tctaccagaa accggttatg
     4981 gctatattcg tcgcggtgaa gtgtctgtgg gtgagcagga tacggtggcc tttgaagtgg
     5041 cgcagtttgt cgaaaaaccg aatctggaaa ccgcccaggc ctatgtagca agcggcgaat
     5101 attactggaa cagcggcatg ttcctgttcc gcgccggacg ctatctcgaa gaactgaaaa
     5161 aatatcgccc ggatatcctc gatgcctgcg aaaaagcgat gagcgccgtc gatccggatc
     5221 tcgattttat tcgtgtggat gaagaagcgt ttctcgcctg cccggaagag tcggtggatt
     5281 acgcggtcat ggaacctacg gcagatgctg ttgtggtgcc gatggatgcg ggctggagcg
     5341 atgttggttc ctggtcttca ttatgggaga tcagcgccca caccgccgag ggcaacgttt
     5401 gccacggcga tgtgattaat cacaaaactg aaaacagcta tgtgtatgcc gaatcaggcc
     5461 tggtcaccac cgtcggggtg aaagatctgg tagtggtgca gaccaaagat gcggtgctga
     5521 ttgccgaccg taacgcggta caggatgtga aaaaagtggt cgagcagatc aaagccgatg
     5581 gtcgccatga gcatcgggtg catcgcgaag tgtatcgtcc gtggggcaaa tatgactcta
     5641 tcgacgcggg cgaccgctac caggtgaaac gcatcaccgt gaaaccgggc gagggtttgt
     5701 cggtacagat gcaccatcac cgcgcggaac actgggtagt ggtcgcggga acggcaaaag
     5761 tcaccattga tggtgatatc aaactgcttg gtgaaaacga gtccatttat attccgctgg
     5821 gggcgacgca ctgcctggaa aacccgggga aaattccgct cgatttaatt gaagtgcgct
     5881 ccggctctta tctcgaagag gatgatgtgg tgcgcttcgc ggatcgctac ggacgggtgt
     5941 aaacgtcgca tcaggcgctg tttgtcggat gcggcgtgaa cgccttatcc gacctacggt
     6001 tcggttttgt aggcctgata agacgcggca gcgtcgcatc aggcaatgaa tgcgaaaccg
     6061 cggtgtaaat aacgacaaaa ataaaattgg ccgtttcggt cagggccaac tattgcctga
     6121 aaaagggtaa cgacatgaaa aaattaacct gctttaaagc ctatgatatt cgcgggaaat
     6181 taggcgaaga actgaatgaa gatatcgcct ggcgcattgg tcgcgcctat ggcgaatttc
     6241 tcaaaccgaa aaccattgtg ttaggcggtg atgtccgtct caccagcgaa accttaaaac
     6301 tggcgctggc aaaaggttta caggatgcgg gcgtcgatgt gctggatatc ggtatgtccg
     6361 gcaccgaaga gatctatttc gccacgttcc atctcggcgt ggatggcggc attgaagtta
     6421 ctgccagcca taatccgatg gattacaacg gcatgaagct ggtgcgcgag ggggctcgca
     6481 cgatcagcgg tgataccgga ctgcgcgacg tccagcgtct ggctgaagcc aacgactttc
     6541 ctcccgtcga tgaaaccaaa cgcggtcgct atcagcaaat caacctgcgt gacgcttacg
     6601 ttgatcacct gttcggttat atcaatgtca aaaatctcac gccgctcaag ctggtgatca
     6661 actccgggaa tggcgcagcg ggtccggtgg tggacgccat tgaagcccgc tttaaagccc
     6721 tcggcgcgcc cgtggaatta atcaaagtac acaacacacc ggacggcaat ttccccaacg
     6781 gtattcctaa cccgctgctg ccggaatgtc gcgacgacac ccgcaatgcg gtcatcaaac
     6841 acggcgcgga tatgggcatt gcctttgatg gcgattttga ccgctgtttt ctgtttgacg
     6901 aaaaagggca gtttatcgag ggctactaca ttgtcggcct gctggcagaa gcgttcctcg
     6961 aaaaaaatcc cggcgcgaag atcatccatg atccgcgtct ctcttggaac actattgatg
     7021 tggtgactgc tgcaggcggc acgccggtaa tgtcgaaaac cggacacgcc tttattaaag
     7081 aacggatgcg caaggaagac gccatctacg gtggcgaaat gagcgcccac cattacttcc
     7141 gtgatttcgc ttactgcgac agcggcatga tcccgtggct gctggtcgcc gaactggtgt
     7201 gcctgaaagg aaaaacgctg ggcgaactgg tgcgcgaccg gatggcggcg tttccggcaa
     7261 gcggtgagat caacagcaaa ctggcgcaac ccgttgaggc gattaaccgc gtggaacagc
     7321 attttagccg tgaggcgctg gcggtggatc gcagcgatgg catcagcatg acctttgccg
     7381 actggcgctt taacctgcgc acctccaata ccgaaccggt ggtgcgcctg aatgtggaat
     7441 cgcgcggtga tgtgccgctg atggaagcgc gaacgcgaac tctgctgacg ttgctgaacg
     7501 agtaatgtcg gatcttccct taccccactg cgggtaaggg gctaataaca ggaacaacga
     7561 tgacaaatct aaaaaagcgc gagcgagcga aaaccaatgc atcgttaatc tctatggtgc
     7621 aacgcttttc agatatcacc atcatgtttg ccgggctatg gctggtttgc gaagtcagcg
     7681 gactgtcatt cctctacatg cacctgttgg tggcgctgat tacgctggtg gtgttccaga
     7741 tgctgggcgg catcaccgat ttttatcgct catggcgcgg tgttcgggca gcgacagaat
     7801 ttgccctgtt gctacaaaac tggaccttaa gcgtgatttt cagcgccgga ctggtggcgt
     7861 tcaacaatga tttcgacacg caactgaaaa tctggctggc gtggtatggg ctgaccagca
     7921 tcggactggt ggtttgccgt tcatgtattc gcattggggc gggctggctg cgtaatcatg
     7981 gctacaacaa gcggatggtc gccgtagcgg gggatttagc cgccggacaa atgctgatgg
     8041 agagtttccg taatcagccg tggttagggt ttgaagtggt gggcgtttac cacgacccga
     8101 aaccgggcgg cgtttctaac gactgggcgg gtaacctgca acagctggtc gaggatgcaa
     8161 aagcgggcaa gattcataac gtctatatcg cgatgcaaat gtgcgacggc gcacgagtga
     8221 aaaaactggt ccatcaactg gcggatacca cctgttcggt gctgctgatc cccgacgtct
     8281 ttaccttcaa cattctccat tcacgtctcg aagagatgaa cggcgttccg gtggtgccgc
     8341 tgtatgacac gccgctttcg ggggttaacc gcctgctaaa acgtgcggaa gacattgtgc
     8401 tggcgacgct tattctgctg ctgatctccc cggtgctgtg ctgtattgcg ctggcggtga
     8461 aactcagttc accagggccg gttattttcc gccagactcg ctatggtatg gatggcaagc
     8521 cgatcaaagt gtggaagttc cgttccatga aagtgatgga gaacgacaaa gtggtgaccc
     8581 aggcgacgca gaacgatccg cgcgtcacta aagtggggaa ctttctgcgc cgcacctcgc
     8641 tggacgaatt gccgcagttt atcaatgtgc tgaccggggg gatgtcgatt gtcggtccac
     8701 gtccgcacgc agtagcgcat aacgaacagt atcgccagct cattgaaggc tacatgctgc
     8761 gccataaagt gaaaccgggc attaccggct gggcgcagat taatggctgg cgcggcgaaa
     8821 ccgacacgct ggagaaaatg gaaaaacgcg tcgagttcga ccttgagtac atccgcgaat
     8881 ggagcgtctg gttcgatatc aaaatcgttt tcctgacggt attcaagggc ttcgttaaca
     8941 aagcggcata ttgatatgag cttacgtgaa aaaaccatca gcggcgcgaa gtggtcggct
     9001 attgccacgg tgatcatcat cggcctcggg ctggtgcaga tgaccgtgct ggcgcggatt
     9061 atcgacaacc accagttcgg cctgctgact gtgtcgctgg tgattatcgc gctggcagat
     9121 acgctttctg acttcggtat cgctaactcg attattcagc gaaaagaaat cagtcacctt
     9181 gaactcacca cgctgtactg gctgaacgtc gggctggggc ttgtggtatg tgtggcggtg
     9241 tttttgttga gtgatgtcat cggcgacgtg ctgaataacc cggacctggc accgttgatt
     9301 aaaacattgt cgctggcgtt tgtggtgatc ccccacgggc aacagttccg cgcgttgatg
     9361 cagaaggagc tggagttcaa caaaatcggc atgatcgaaa ccagcgcggt gctggcgggc
     9421 ttcactttta cggtagttag cgcccatttc tggccgctgg cgatgaccgc gatcctcggt
     9481 tatctggtca atagtgcggt gagaacactg ctgtttggct actttggacg caaaatttat
     9541 tgccccggtc tgcacttctc gctggcgtct gtggcaccga acttacgttt tggtgcctgg
     9601 ctgacggcgg acagcatcat caactatctc aacaccaacc tttcaacgct ggtgctggcg
     9661 cgtattcttg gcgcgggcgt ggcaggggga tacaacctgg cgtacaacgt ggccgtcgta
     9721 ccgccgatga agctgaaccc aatcatcacc cgcgtgttgt ttccggcgtt tgccaaaatt
     9781 caggacgaca ccgaaaagct gcgtgttaac ttctacaagc tgctgtcgat agtggggatt
     9841 atcaactttc cggcgctgct ggggctgatg gtggtgtcga ataacttcgt accgctggta
     9901 ttcggcgaaa agtggaacag cattattccg gtgctgcaat tgctgtgtat tgtgggtctg
     9961 ctgcgctccg tgggtaaccc gattggttcg ctgctgatgg cgaaagcgcg ggtcgatatc
    10021 agctttaaat tcaacgtatt caaaacattt ctgtttattc cggcgattgt tattggtggg
    10081 cagatggcgg gcgcaatcgg cgtcacgctc ggtttcctgc tggtgcagat tatcaacacc
    10141 attctgagtt acttcgtgat gattaaaccg gttcttggtt ccagttatcg ccagtacatc
    10201 ctgagtttgt ggctgccgtt ttatctctcg ttgccgacgc tggtggtcag ttatgcgctg
    10261 ggcattgtgc tgaaagggca actggcgctg gggatgctgc tggcggtgca aatagccgcg
    10321 ggcgtgctgg cgtttgtggt gatgattgtg ctgtcacgcc atccgctggt ggtggaagtg
    10381 aagcgtcagt tttgtcgcag cgaaaaaatg aaaatgcttt tacgggcggg gtgaattgct
    10441 gttttcggtt ccgacaacgg ttgccggatg cggcgtaaaa cgccttatcc gtcctacggt
    10501 tctgtgctcg ggacctgtag gcctgataag acgcgccagc gtcgcatcag gcaaaaaacg
    10561 gcaacgaaca caacaaacac agcctcacct ggcatcaaat ttatgaggaa aaaatgaaat
    10621 tacttattct gggcaaccac acttgcggca atcgtggcga cagcgccatc ttgcgcggct
    10681 tacttgatgc catcaacatc ctcaatccac ataccgaagt ggacgtgatg agccgctatc
    10741 cggtcagttc ttcctggctg ctcaaccgcc cggtaatggg cgatccgctg ttcctgcaga
    10801 tgacacaaca caatagcgcg gcgggcgttg tcgggcgcat taaaaaagtc ctccgtcgcc
    10861 gctaccagca tcaggtattg ctctcacgcg tcaccgacac tggcaagctg cgcaatatcg
    10921 ccatcgccca gggattcacc gacttcgtgc gcctgctgtc aggttacgac gccattattc
    10981 aggtcggcgg atcgtttttt gtcgatctct acggcgtgcc gcagtttgaa catgcgcttt
    11041 gcacatttat ggcgaaaaag ccgctgttta tgattggtca cagcgtcggc cccttccagg
    11101 atgagcaatt taaccaactg gcgaactatg tctttggtca ctgcgacgcg ctgatcctgc
    11161 gcgaatcggt cagccttgat ttgatgaaac gcagaaatat caccaccgca aaagtggaac
    11221 atggcgtcga taccgcgtgg ctggtcgatc accacaccga agacttcacc gccagctatg
    11281 ccgttcaaca ctggctggac gttgccgcac aacagaaaac agtggcaatt accctgcgcg
    11341 aactggcacc gttcgacaaa cgtctcggca ccactcaaca agcgtatgaa aaagcctttg
    11401 ccggggtggt caatcgcatt ctcgatgaag gctatcaggt gattgcgctc tccacctgta
    11461 cgggtatcga cagctataac aaagacgacc gcatggtggc gctcaatctg cgccagcaca
    11521 tcagcgatcc tgcccgttac cacgtagtga tggatgaact caacgatctg gaaatgggca
    11581 aaattctcgg agcctgtgaa ctcaccgtcg gtacgcgcct gcactccgcc attatctcaa
    11641 tgaactttgc tactccggcg attgccatca actatgaaca taaatccgcc gggattatgc
    11701 agcagctggg actgccagag atggcgattg atattcgtca tttactggac ggcagcctgc
    11761 aagcgatggt tgcggatacc ttaggccagc ttccggtgct gaacgctcga tttaacgaag
    11821 ccgtcagtcg cgaacgtcag acgggaatgc agatggtgca gtctgtgctt gagcgcatcg
    11881 gggaggtgaa atgaaggtcg gcttcttctt attgaaattc ccgctgtcgt cggaaacctt
    11941 cgtcctcaat caaattaccg cgtttattga tatgggcttt gaagtggaga ttgtcgcttt
    12001 gcaaaaaggc gatacagaga acacccacgc ggcatggacg aaatacaacc ttgtcgccag
    12061 aacccgctgg ttacaggacg aacctgcggg caaagtagcg aaactgcgcc accgcgccag
    12121 ccagacctta cgtggcattc atcgtaaaaa tacctggcat gcgctcaacc tcaaacgcta
    12181 tggtgccgag tcgcggaacc tgattttgtc tgccatttgt ggtcaggtcg caacaccgtt
    12241 tcgcgccgat gtgttcatcg ctcattttgg cccggcgggg gtaaccgcag caaaactacg
    12301 cgaactgggt atcattcgcg gcaaaattgc caccatcttc cacggtattg atatctccag
    12361 tcgggaagtg ctcaaccact acactcccga atatcaacaa ctgtttcgcc gtggcgacct
    12421 gatgttaccg ataagcgatc tgtgggccgg aaggctgcaa aaaatgggct gccccagaga
    12481 aaaaatcgcc gtatcgcgta tgggcgtgga catgacgcgc tttagcccgc gtccggtgaa
    12541 agcgcccgca acgccgctgg aaatcatatc tgtcgcacgc ttaaccgaga aaaaaggcct
    12601 gcatgtggcg attgaagcct gccgtcagtt gaaagagcag ggcgtggcat ttcgctatcg
    12661 catcctcggc attggcctgt gggaacgacg cctgcgcacc atcatcgaac aatatcaact
    12721 ggaagatgtg gtagagatgc cgggctttaa accgagccat gaagtgaagg agatgctcga
    12781 cgacgcggat gtcttcctgt tgccatcggt aacgggcgcg gatggcgata tggaaggcat
    12841 tccggtggcg ctaatggaag cgatggcggt cggtattccg gtggtttcta ctctgcatag
    12901 cggaattcct gaactggtgg aggccgataa atccggctgg ctgctgcctg agaacgatgc
    12961 tcgcgcactg gcacaacgtc tggcggcgtt tagccaactg gataccgacg aactggctcc
    13021 ggtcgtcaaa cgtgcgtgcg aaaaagtcga acacgatttt aaccagcagg tgattaatcg
    13081 agaactcgcc agcttgctgc aggctttata gaggagaacg tatgccattt aaaacactct
    13141 cccgacgcac cttcctgacg gcaagctcgg cgcttgcctt cctccatacc cctttcgctc
    13201 gcgcacttcc cgcccgacaa agcgttaaca ttaacgacta caacccacat gactggatcg
    13261 cctcatttaa acaagccttc agcgaagggc aaacggtcgt cgtgcctgcc ggattcgttt
    13321 gtgacaatat caacaccggc atcttcatcc ctcccggcaa aacgttacac atccttggaa
    13381 gcctgcgcgg caacggcaga gggcgatttg tcttacagga cggcagccag gtgacagggg
    13441 gagagggcgg cggtatgcat aacattaccc tggatgtgcg tggttctgac tgcaccatca
    13501 aagggctggc gatgagcggc tttggcccgg taatgcagat ttatattggc ggcaaaaaca
    13561 aacgggtcat gcgcaacctg accatcgata acctcactgt cagccacgct aattacgcca
    13621 tcttacgcca gggatttcat aaccagatta tcggtgccaa cattaccaac tgtaagttca
    13681 gcgacttaca gggcgacgcc attgaatgga acgtggcaat taacgacagt gatattttga
    13741 tatctgacca tgtcatcgag cgtatcaact gtaccaacgg caaaatcaac tggggcatcg
    13801 gcataggcct tgcgggaagc acttatgata acaactaccc ggaagaccag gcagtgaaaa
    13861 actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat gttgaaaatg
    13921 gtaaacattt tgttattcgt aatatcaatg cccgcaatat cacgccggat ttcagtaaga
    13981 aagcgggcat tgataacgcg acagtcgcta tttacggttg tgacaatttc gtgattgata
    14041 atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt aaaggcaaat
    14101 atctctcgat accgcaaaat ttccgagtga ataatattca actggataat acccatcttg
    14161 cttataaatt gcgcggcatc caaatctccg ccgggaatgc cgtctccttt gtgtcgctaa
    14221 ctaacattga gatgaagcgt gcctcgctgg agttacacaa caagccgcaa catcttttta
    14281 tgcgaaatat caatgtgatg caggaatcct cagttggacc agcattgagc atgaacttcg
    14341 acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaaa agaaacactg ctgtctcttg
    14401 caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac aggattaatc
    14461 accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg gagtagattt
    14521 gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca atccacaacg
    14581 aagcggcgta acatcacaag taattcagta atcaattcag ggtaattgat gctggcgaaa
    14641 aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata atgacgaatt
    14701 taaaagcagt tattcctgta gcgggtctcg ggatgcatat gttgcctgcc actaaggcga
    14761 tacccaaaga gatgctacca atcgtcgaca agccaatgat tcagtacatt gttgacgaga
    14821 ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag aacgcggtcg
    14881 aaaaccactt cgacacctct tatgagttag aatcactcct tgagcagcgc gtgaagcgtc
    14941 aactgctggc ggaagtacag tccatctgtc cgccgggcgt gatcattatg aacgtgcgtc
    15001 agggcgaacc tttaggttta ggccactcca ttttgtgtgc gcgacctgcc attggtgaca
    15061 acccatttgt cgtggtactg ccagacgttg tgatcgacga tgccagcgcc gacccgctac
    15121 gttacaacct tgctgccatg attgcacgtt tcaacgaaac gggccgcagc caggtgctgg
    15181 caaaacgtat gccgggtgac ctctctgaat actccgtcat ccagactaaa gagccgctgg
    15241 accgtgaggg taaagtcagc cgcattgttg aatttatcga aaaaccggat cagccgcaga
    15301 cgctggactc agacatcatg gccgtaggtc gctatgtgct ttctgccgat atttggccgg
    15361 aactggaacg tactcagcct ggtgcatggg gacgtattca gctgactgat gctattgccg
    15421 agctggcgaa aaaacaatcc gttgatgcaa tgctgatgac cggcgacagt tacgactgcg
    15481 gcaaaaaaat gggctatatg caggcgtttg tgaagtatgg cctacgcaac ctgaaagaag
    15541 gggcgaagtt ccgtaaaggt attgagaagc tgttaagcga ataatgaaaa tctgaccgga
    15601 tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcataa gttttgctca
    15661 ttgaattttc ctgccgttgc cgtttttata aagcctataa aaacaagaag ttacatagct
    15721 catattattc aaatttttcc aggattttcc ttgtttctac ggctgtttgg taagacaatt
    15781 aacgtttgaa tttttcggat ttggcgcgag tggctaacgc tcgccacatc gttgagatgc
    15841 atgcagtgca ctggtagctg ttaagccagg ggcggtagcg tgtattaata cctctattaa
    15901 tcaaatctgg agcagtctat ttcacagcat gctctatagc tatatggaat aaaaaagtga
    15961 agatacttgt tactggtggc gcagggttta ttggttctgc tgttgttcgt cacataataa
    16021 ataatacgca agatagtgtt gttaatgtcg ataaattaac atacgccggg aacctggaat
    16081 cactcgctga tgtttctgat tctgaacgtt atgcttttga gcatgcagat atctgcgatg
    16141 ctgtagccat gtcgcgtatt ttcgcacagc accagccaga cgcggtgatg cacctggcgg
    16201 cagagagcca cgttgaccgt tcaataacgg gccctgcggc atttattgaa accaatattg
    16261 tggggactta tgttctttta gaagcggcgc gcaattactg gtctgctctg aatgatgaaa
    16321 agaaaaaaag tttccgcttt catcatattt ctactgatga agtttatggt gacttacccc
    16381 atccggatga agcaaataat aacgaagcgt taccgctatt tacggaaacg acagcttacg
    16441 cgccaagtag cccgtattct gcgtctaaag cttccagcga tcatttggta cgtgcctgga
    16501 agcggactta tggtttacca accattgtga ctaattgctc gaacaattat ggtccttatc
    16561 acttcccgga aaaacttatt ccattggtta ttctgaatgc tctggaaggt aaggcattac
    16621 ctatttatgg taaaggggat caaattcgcg actggttgta tgtagaggat catgctcgtg
    16681 cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacata
    16741 acgaaaagaa aaacatcgac gtagtgctca ctatttgtga tttgttggat gagattgtac
    16801 cgaaagagaa atcttatcgt gagcaaatta cttatgttgc cgatcgcccg gggcatgacc
    16861 gccgttacgc cattgatgcc gataaaatta gccgcgaatt gggctggaaa ccgcaagaaa
    16921 cgtttgagag cgggattcgt aaaacggtgg aatggtatct ggctaataca aattgggttg
    16981 agaatgtgaa aagcggtact tatcagtcgt ggattgaaca aaactatgag ggccgtcagt
    17041 aatgaatatc ctgcttttcg gcaaaacagg gcaggtgggt tgggaactgc agcgtgctct
    17101 ggctcctctg ggtaacttga ttgcgctcga tgttcactcc actgattatt gtggagattt
    17161 cagcaacccc gaaggtgtgg ctgaaaccgt caaaaaaatt cgccccgatg ttattgttaa
    17221 tgctgctgct cacactgcag tagacaaagc ggaatcagaa ccgaatttcg cgcaattact
    17281 taacgcgaca tgcgtcgaag cgattgcaaa agcagctaat gaagtcggag cctgggttat
    17341 acactactcc actgattatg ttttcccagg caacggtgac acgccatggc tggaaacgga
    17401 tgcaacagca ccgctaaatg tctacggtga aaccaagcta gctggggaaa aagcgttaca
    17461 agaacattgc gcaaagcatc ttattttccg taccagctgg gtatacgctg gtaaaggaaa
    17521 taactttgcc aaaacgatgt tgcgcctggc aaaagaacgc gaagagttgg ctgtaataaa
    17581 cgatcaattc ggcgcaccaa cgggggctga attactggct gattgcaccg ctcatgccat
    17641 tcgcgtggcc gcaaataaac cagaagttgc tggtttgtac catctggttg ctggtggtac
    17701 aacaacctgg cacgattatg ccgctctggt atttgaagaa gcgcgcagag cggggatcaa
    17761 ccttgctctt aacaaactta atgccgtgcc gacaacggcc tatcccacac cagcacgtcg
    17821 tccccataat tctcgcctca ataccgaaaa atttcagcag aactttgcgc ttgtcttgcc
    17881 tgactggcag gtgggcgtga aacgtatgct caacgaatta tttacgacca cggcaattta
    17941 acaaattatt gcatctcgct catgatgcta gagcgagatg aattaaaagg aatggtgaaa
    18001 tgaaaacgcg taaaggtatt attctggctg gtggttccgg cactcgtctt tatcctgtga
    18061 caatggctgt gagcaaacaa ctgctaccga tttatgataa accgatgatt tattatccgc
    18121 tttcaacgct tatgttggcg ggtattcgag atattctgat tatcagtacg ccgcaggata
    18181 caccacgttt tcaacaattg cttggtgatg gaagccagtg ggggcttaat ctgcaatata
    18241 aagtacaacc gagtccggat ggtctggcgc aagcatttat cattggtgaa gaatttatag
    18301 gtggtgatga ttgtgcgctg gtacttggtg ataatatctt ctatggtcac gacctgccga
    18361 aattaatgga taccgctgtt aacagagaga gtggcgcaac ggtttttgcc tatcacgtaa
    18421 atgatcctga gcgctacggt gtggtggaat ttgatgataa cggtacggca attagtctgg
    18481 aagaaaaacc gcaggaacca aaaagtaact atgcggttac tgggctttat ttctatgaca
    18541 atgacgttgt agaaatggcg aaaaacctta aaccatctgc acgtggcgaa ctggaaatta
    18601 ccgatattaa ccgtatttat atggaccaag gacgtttgtc tgtcgctatg atggggcgtg
    18661 gttatgcatg gctggacacg gggacacatc aaagtcttat tgaagcaagc aacttcattg
    18721 caacaattga agagcgtcag gggctgaaag tttcctgccc ggaagaaatt gctcaccgta
    18781 aaggctttat tgatgctgag caagtgaagg tattagccga gccgctgaag aaaaatgctt
    18841 atggtcagta tctgctgaaa atgattaaag gttattaata aaatgaacgt aattaaaact
    18901 gaaattcctg atgtgctaat ttttgaacca aaagtatttg gtgatgagcg gggctttttt
    18961 atggaaagct tcaatcagaa agttttcgaa gaggctgtag gacgaaaggt tgagtttgtt
    19021 caggataacc attctaaatc aactaagggt gtgttacgcg gactgcacta tcagctggaa
    19081 ccttatgctc aaggaaaact ggtacgctgt gttgtcggtg aagtttttga cgtagctgtt
    19141 gatattcgga aatcatcacc aacatttggc aagtgggttg gtgtaaattt gtcggcggag
    19201 aataagcgtc aattatggat accggaagga ttcgctcatg ggttttgcgt tcttagtgat
    19261 gaagcagaat ttgtctacaa aacaaataat ttctattcaa aaatgcaaga gcgtgggatt
    19321 tt
//
LOCUS       DAZSNM010000065        19230 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZSNM010000065 DAZSNM010000000
VERSION     DAZSNM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19230
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.065"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            88..1671
                     /locus_tag="ACHW42_001895"
     CDS             88..1671
                     /locus_tag="ACHW42_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR8943735.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2123..3976)
                     /gene="asmA"
                     /locus_tag="ACHW42_001896"
     CDS             complement(2123..3976)
                     /gene="asmA"
                     /locus_tag="ACHW42_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR8943736.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3998..4579)
                     /gene="dcd"
                     /locus_tag="ACHW42_001897"
     CDS             complement(3998..4579)
                     /gene="dcd"
                     /locus_tag="ACHW42_001897"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR8943737.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4671..5312)
                     /gene="udk"
                     /locus_tag="ACHW42_001898"
     CDS             complement(4671..5312)
                     /gene="udk"
                     /locus_tag="ACHW42_001898"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR8943738.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5630..5872
                     /locus_tag="ACHW42_001899"
                     /pseudo
     CDS             5630..5872
                     /locus_tag="ACHW42_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            5871..6812
                     /gene="mdtA"
                     /locus_tag="ACHW42_001900"
                     /pseudo
     CDS             5871..6812
                     /gene="mdtA"
                     /locus_tag="ACHW42_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            6812..9934
                     /gene="mdtB"
                     /locus_tag="ACHW42_001901"
                     /pseudo
     CDS             6812..9934
                     /gene="mdtB"
                     /locus_tag="ACHW42_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            9935..13000
                     /gene="mdtC"
                     /locus_tag="ACHW42_001902"
     CDS             9935..13000
                     /gene="mdtC"
                     /locus_tag="ACHW42_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR8943739.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            13001..14416
                     /locus_tag="ACHW42_001903"
     CDS             13001..14416
                     /locus_tag="ACHW42_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8943740.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            14413..15816
                     /gene="baeS"
                     /locus_tag="ACHW42_001904"
     CDS             14413..15816
                     /gene="baeS"
                     /locus_tag="ACHW42_001904"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR8943741.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            15813..16535
                     /gene="baeR"
                     /locus_tag="ACHW42_001905"
     CDS             15813..16535
                     /gene="baeR"
                     /locus_tag="ACHW42_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR8943742.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            16726..17058
                     /locus_tag="ACHW42_001906"
     CDS             16726..17058
                     /locus_tag="ACHW42_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR8943743.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            17205..18566
                     /gene="yegQ"
                     /locus_tag="ACHW42_001907"
     CDS             17205..18566
                     /gene="yegQ"
                     /locus_tag="ACHW42_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR8943744.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(18908..19225)
                     /gene="yegR"
                     /locus_tag="ACHW42_001908"
     CDS             complement(18908..19225)
                     /gene="yegR"
                     /locus_tag="ACHW42_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR8943745.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         4319 a         5203 c         5161 g         4547 t
ORIGIN      
        1 tagtgctgca acattgcatt tttgccccga tttatccatg atcgaattgt gacatttgtc
       61 atacaacgaa taggttttgt acttactatg gaatggattg ccgatccgtc tatctgggcc
      121 gggttaatca cgctgattgt gatcgaactg gtcctcggca ttgataacct ggtctttatt
      181 gccatcctcg ccgaaaaact accgccgaag cagcgcgacc gcgcacgggt taccgggctg
      241 ctgctggcga tgttaatgcg cctgttactg ctagcgtcaa tctcctggct ggtcaccctg
      301 actcaaccgc tgttcagctt ccgctcgttt acctttagcg cccgcgactt aatcatgctg
      361 tttggtggtt tcttcctgtt gttcaaagcc acgatggagc tgaacgaacg gctggaaggg
      421 aaagacagca ataatcccac acaacgcaaa ggtgcgaagt tctggggcgt ggtgacgcaa
      481 attgtggtac tggacgccat tttctcactc gactcagtga ttaccgccgt cgggatggtc
      541 gaccatttac tggtcatgat ggccgccgtg gttatcgcta tcagcctgat gttgatggcc
      601 agcaagccgt taacgcaatt tgttaacagt cacccgacca tcgttattct ctgcttaagc
      661 ttcctgttga tgattggctt tagcctggtg gcagaaggtt tcggcttcgt cattccgaaa
      721 ggctacctgt acgctgccat tggtttctcg gtgatgatcg aggcactcaa tcagttggct
      781 atctttaacc ggcgacgttt tctttccgct aatcagacgc tacgccagcg gactaccgaa
      841 gcggtaatgc gcctgcttag cgggcaaaaa gaagatgcgg aactggacgc caaaaccgcg
      901 tccatgctgg tggatcatgg aaaccagcag atctttaatc cgcaggaacg gcggatgatt
      961 gagcgggtac ttaatcttaa ccagcgtacc gtaagcagca ttatgacgtc gcgccacgat
     1021 attgagcata tcgatctcaa cgcgccggaa gaagaaatcc gccagttgct ggagcgaaat
     1081 cagcatacgc ggctggtggt taccgacggt gatgacgcag aagatttgct cggtgttgtt
     1141 cacgttatcg acctgctaca acagtcactg cgcggcgaac cgctcaacct gcgggtgttg
     1201 attcgccagc cgctggtgtt cccggaaacc ttgccgttgt taccagccct ggagcagttc
     1261 cgtaatgccc gcacgcactt tgcttttgtg gtggatgagt ttggctcggt ggaagggatt
     1321 gtgacattaa gtgacgtcac tgaaaccatt gccggtaact taccgaacga agtggaagag
     1381 atcgacgccc gccatgatat tcagaagaat gcggacggtt cctggacggc gaatggtcat
     1441 atgccgctgg aggatctggt gcaatatgtg ccgctgccgc tggatgaaaa acgtgaatat
     1501 cacaccattg ccgggctgtt gatggagtat ttgcagcgta ttccaaaacc tggcgaagaa
     1561 gttcaggtgg gggattattt gcttaaaacg ttgcaggtgg aaagccatcg cgtgcagaag
     1621 gtgcagatta taccgctgcg taaggatggc gagatggagt acgaggtgtg atggtatcgg
     1681 ccattttacc caatgctgcg taacgcctta taacttcagc aatgaagcgt tgtctgtggt
     1741 aaacggacat cacagcgggg gtgtgggtcc ggctgtaaat cgccgaatcg tttcctgtag
     1801 gctggggcag cccacatgga tgtgggctga gggcgcgttt tacagggatg ttacctcgcg
     1861 cccgtacccg atagccgaaa ggaataagat gagggaaccg cgccagcggc gatttaccgc
     1921 cgggagcccg ggttgccagg gtggtggcga ttgagccacc ctggcacgtt caccggcttg
     1981 agcgttccat agtagcgagg aacatcaagt gaacggaaca gccttcgatc ttacatattc
     2041 agaatataat agtgccgccg cgaacgcctg atgcgacgct ttacgcgtct tatcaggcct
     2101 acaggacagg tcacctggta acctacatct tctccagcaa cttcttcaca tctttgccat
     2161 tgcgggaatc tttattccgc tcggcccagt cattcaggcg acgtttcgct tcgtcctgta
     2221 gatgtttgcg cagtaactga tccacttgca ggctgtaatt gagttgctgc cagttgccat
     2281 aaacccgcag cggtactggc gtttctttga ggaaatcaat cagtttgctt tccccgttcc
     2341 agccacccac gacccgaata tcaaactggg tgtcgcaggt ttgatctgcc agattcaaca
     2401 tgccttcccc tgtcagcgcc agcacaggcg attgaccttg catgtcgttt aacgtcacga
     2461 cgccatcctt caacgtcaaa tcggtggtaa agcggtcaag ccgcgtcacg ttatcgaagt
     2521 tttcagcggc cttcacatca ccgccattac gctctaccgc ttgctgaatc atctgctgga
     2581 agttcatccc ttccatacgt gtgtcggtca tctcaacatg cgcctgtcct tgccagttgt
     2641 ggcggaatgc gtcggcatct atgtcagcac cggagaaatc accagccagt gacatttttc
     2701 cggtcaacga aatcggatag ttaaacgcct tcagaatggt accaatctca acgttctcca
     2761 gccgtggctg gaagtttatc cgcggattta ttgatgtcgc gtccagcgtg cccggcagtg
     2821 aaacctttcc accgttaagc ttgccctgca gttgagtaat ttccagcaaa cccgacttgt
     2881 tggtcatttg cgtggcaaca tctgtaaaat tcattccgcg ccagcgcacg ttactggcct
     2941 gcaacaaaat atcagctgta aagccttgca gtccctgata ggccggttca tcaatacgcg
     3001 aagaaatgac cgggcgcggc aacgttgatt ggctctgccc ctgctgcgcg gcaccgtttt
     3061 caccattcgc ggtttcatta agcgggatga ggttgtcaag attcagttgc gggaattgca
     3121 gtctcagctg ccattccggt ttctctgtca gcgtgacctg tgcttgtccg ctcagcgtac
     3181 tgtcattggc ggtcaaacta atttggttaa atgaaaggcg tttatgtgac tcctgccact
     3241 gggactggaa actcccctgc ccctgaatac cttgttttgg taaatcggca ccctgcaact
     3301 gccagttaat ttgttcaata gccgctgtta aatcatgcgg ataatcagaa gcatctaccg
     3361 taccgttaag ggatatcgtg agatcgcgct gatcgcgatt aacccgcccg gagaactcaa
     3421 atgagccacg atgttgggga tcttgttcca tttgcaggcg gatattgcgg attgtcacct
     3481 gctcgtcatc ttcatgctgg aacaccagaa cgctgtccgc caccttaaga ctggatatat
     3541 caaacgacca tccgcgatcg tctgacagat ccggcaaggt attgtcgcgc ggtgcaaccg
     3601 gagcgtcttc actgcgcacc gcttccgtct gcggcgtcag ttggatcact gcccctttta
     3661 gcatcacctg cttaacgctc agttgatgac tcagtagtgg taaaagcgcc acgtccagac
     3721 gcatgttgtc ggcgcgaacc agtggctggc ttgcgccctg ggcggtgaga gacattcgcc
     3781 cggagaggat actaagctgc ggccagacgt gccaacgcag tggcccgtcg agctgcaatt
     3841 gataaccgct acgtgcagca acttgcttga ccatatagtc gcggaaatca ttcggattca
     3901 ccagcaacac taacgcagat aacccggcca ccagcacgac caagagtatc atcagcgtcg
     3961 tcagaaatcg tctcatggta tcctcaatgg gcctgaatta gtctttatcg attcggctgg
     4021 ctaccgcgcc ctgctggttg cgatatttcg catcttcacg gcggttgtaa ggtcgcgccg
     4081 ccgggccgga aagcggctca aagctcagcg caccaattaa catgcccgga cgcagcgcca
     4141 gcggcagctt accggagttg tagaactcca gcacaatgca accagaccag cccggatcga
     4201 tacggtgcgc ggtgacgtgc accatcagcc ccagacgcgc caaagaggaa cgcccgtcca
     4261 gccagcccac cagatcggct ggcagcgtca ccgactccag tgtcaccgcc agcgccagct
     4321 ctcctgggtg aagatagaac gcctcgccct cgtcgagaac gatctcatcg ctcatcacgc
     4381 ggtcaagcgc ggcgctcact tcatctttgg gaccgctcag atcgataaac gctgccgtgt
     4441 gaccacggaa ggtacgaaat ttattgccca ggcgtacatc caccgtcgcg ccgttaatac
     4501 gctccactgg cggacgtggg ttgatcgaca aacggccttc atcaagccag gcttcaatat
     4561 ctcggtcaca cagacgcatg gcattttctc ctttcgcaca tcactccctt aacgccaatt
     4621 acgtcaaggg cataccaact tatcactgaa cggtacacaa tttatcaagc ttattcaaag
     4681 aactgactta ttttcgcttt caatatatcg atcgcgatgc ggtttttccc gccgcgcggc
     4741 acgataatgt ccgcatattg tttagaaggc tcaatgaatt gcaggaacat cgggcgcacg
     4801 gttttttgat attgcgccat cacggaatcc attgaacgcc cacgctcgtt aacgtcacgc
     4861 ttgatgcggc gcatcaggca gatatccagc ggggtatcaa cgaaaatgga gaagttaagt
     4921 tcgtcacgca aacgcgcatc cgtcagcaac aaaatgcctt cgagaatgat gaccttcttc
     4981 ggctcaaccg tcaccgtttc tttcatacgc gtatgttcaa catagctgta aaccggcagg
     5041 tcaattgccg agccgcgttt caacgcttgt aaatgctcaa gcaacaggct gtgatccatc
     5101 gcgctgggat ggtcgtagtt ggtcttaacg cgttcttcca tcgacagatg gctttgatct
     5161 ttgtaatagc agtcttcggg aattacgccg atgtgttcat caccgacttg ctcacgtaat
     5221 tcacgataaa gggtactggc aataagactc ttgccggaag ccgatgcgcc agcgataccg
     5281 ataatgacgc actgatgaga ctgatcagtc atatatttag cgacctgatt aacctggatg
     5341 ttaggaaggg ggcgacgaag cgccaaacgc ggcaattata gggatttcat ccgtctgata
     5401 ccagtcgaat agcgttgccg cgcgctcaga gttaattgtt gacaaagaat tcccggtggc
     5461 aaattacgtt gatcagtttt atgtaaggta aaaaatgtta tacgcagttg cgcaaattat
     5521 ccgcctttac gtcactttat gagcaattcg catataaaat gtaaaacttt tgtactagca
     5581 taaacacaga aacgaatact ggcgacctgg ccttgcggat aaagcggtaa taagcaaaca
     5641 atcacagcat gtattaattg ccctgcccca cccgctgctt cacctggtca gtttaggttt
     5701 agtctcgttt atctttaccc ttgtctcgct tgagctttcg cagtttggca cccaactcgc
     5761 cccactgtgg ttcccgacgt ccatcatgat ggtggcgttt tatcgccatg ccgggcgcat
     5821 gtggccggga attgcgctga gctgttcgct gggaaatatc gccgcatcca ttccaggaag
     5881 gccagcaggt caaagcaggc gatttactgg cagaaattga ccccagccag ttcaaagttg
     5941 cattagcaca aacccagggc caactggcaa aagataaagc cacgcttgct aacgcccgcc
     6001 gtgatctggc gcgttatcag caactggcaa aaaccaatct cgtctcccgt caggagctgg
     6061 atgcccaaca ggcgctggtc agtgaaaccg aaggcaccat taaggctgat gaagcaagcg
     6121 tcgccagcgc gcagttgcaa ctcgactgga gccgcatcac cgcaccagtc gatggtcgcg
     6181 ttggtctcaa gcaagttgat gttggtaacc aaatctccag tggtgatacc accggaattg
     6241 tggtgatcac ccagacgcat cctatcgatt tagtctttac cctgccggaa agcgatatcg
     6301 ctactgtagt gcaggcgcaa aaagccggaa aaccgctgat ggtagaagcc tgggatcgca
     6361 ccaattcgaa gaaattaagt gaaggcacgc tgttaagcct cgataaccaa atcgatgcca
     6421 caaccggtac gattaaagtg aaagcacgct ttaataatca ggatgatgcg ctgtttccca
     6481 atcagtttgt taacgcgcgc atgttagtcg acaccgaaca aaacgccgta gtgatcccaa
     6541 cagccgccct gcaaatgggc aatgaaggcc attttgtctg ggtgctgaat agcgaaaaca
     6601 aggtcagcaa acatctggtg acgccgggca ttcaggacag tcagaaagtg gtgatccgtg
     6661 caggtatttc tgcgggcgat cgcgtggtga cagacggcat tgatcgcctg accgaagggg
     6721 cgaaagtgga agtggtggaa gcccagagcg ccaccactcc ggaagagaaa gccaccagcc
     6781 gcgaatacgc gaaaaaagga gcacgctcct gatgcaggtg ttacccccga gcagcacagg
     6841 cggcccgtcg cgcctgttta ttatgcgtcc tgtcgccacc acgctgctga tggtggcgat
     6901 cttactcgcc gggattatcg gttatcgcgc cctgcccgtt tcggcgctgc cggaagtgga
     6961 ctatccgacc attcaggtgg tcacgctcta cccaggtgcc agcccggatg tcatgacctc
     7021 tgccgttacc gcgccgctcg aacgccagtt cgggcagatg tctggcctga aacagatgtc
     7081 gtcgcaaagt tccggcggtg cgtcagttat cactttgcag ttccagctaa cattaccgct
     7141 caatgtcgcc gagcaggaag tgcaggccgc gattaacgct gcgaccaact tgttgccgag
     7201 cgatctgcct aacccgccgg tttacagcaa agtgaacccg gcagatccgc cgatcatgac
     7261 gctcgccgtc acctcaaccg ccatgccgat gacgcaagtg gaagatatgg tggaaacccg
     7321 cgtcgcgcag aaaatctcgc agatttccgg cgtcggcctg gtgacgcttt ccggcggtca
     7381 gcgtccggct gttcgcgtca aacttaacgc tcaggcgatt gccgccctcg gcctgaccag
     7441 cgaaaccgtg cgcaccgcca ttaccggcgc taacgttaac tcggcaaaag gtagcctcga
     7501 cggcccttcc cgtgcggtca cgctttccgc gaacgaccag atgcaatccg ccgaagagta
     7561 tcgccagcta atcatcgcct accagaacgg cgcgccaatt cgtctgggcg atgtcgcaac
     7621 tgtagagcaa ggtgcagaaa acagctggct cggcgcgtgg gcgaacaaag aacaggccat
     7681 tgtgatgaat gttcagcgcc agcccggtgc taacattatc tccaccgccg acagcattcg
     7741 gcagatgctg ccacagctca ctgagagtct gccgaaatcg gtgaaggtga cagtgctttc
     7801 cgatcgcacc accaatatcc gcgcatccgt cgatgatact cagtttgaat tgatgatggc
     7861 tatcgcgctg gtagtcatga ttatctacct gtttttgcgc aatattccgg cgaccatcat
     7921 tcccggtgtt gctgtaccgc tgtcgttaat cggcactttc gcggttatgg tgtttctcga
     7981 tttttcaatc aataacctga cactgatggc tttaactatc gccaccggat tcgtggtcga
     8041 tgacgccatc gtggtgatcg aaaacatttc ccgctatatc gaaaaaggcg aaaaaccgtt
     8101 ggcggcggcg ctcaagggcg caggtgaaat cggctttacc attatctcgc tgaccttctc
     8161 actgattgcg gtgttgatcc cactgctgtt tatgggcgat atcgtcgggc gactgttccg
     8221 cgaatttgct attaccctgg cggtagcgat tttgatctca gcggtggtgt cgctgaccct
     8281 gacaccgatg atgtgcgcgc ggatgctcag ccaggagtcg ttgcgtaaac agaacagctt
     8341 ctcccgtgcc tcggaaaaaa tgttcgacag gataatcgcc gcctatggtc gtggactggc
     8401 gaaagtgctg aatcatccgt ggctgacctt aagcgtggca ctcagcacgc tgctgcttag
     8461 cgtgctgctg tgggtgttca ttccgaaagg tttcttcccg gtacaggaca acggcattat
     8521 tcagggcact ttgcaggcac cgcaatccag ctcctttgcc aatatggccc agcgacaacg
     8581 ccaggtcgcg gacgtgattt tgcaggatcc ggcagtgcaa agcctgacct catttgttgg
     8641 cgttgatggc actaacccgt cgctgaatag tgcacgttta caaatcaacc tcaaaccgtt
     8701 ggatgaacgt gatgatcggg tgcaaaaagt catcgcccgt ctgcaaacgg cggtagataa
     8761 agtgccgggc gtcgatctct tcctgcaacc aacgcaggac ctgaccatcg atactcaggt
     8821 cagccgcacc cagtaccagt ttaccttgca ggccacgtca ctggatgcgc tcagtacctg
     8881 ggtgccacag ttgatggaaa aactccagca actgccacag ctttctgatg tctccagcga
     8941 ctggcaggac aaagggctgg tggcgtatgt caatgttgat cgcgacagcg ccagccgtct
     9001 ggggatcagc atggcggacg tcgataacgc cctgtacaac gcgtttggtc agcgactgat
     9061 ttccactatt tatactcagg ctaaccagta tcgcgtggtg ctggagcaca ataccgaaaa
     9121 taccccaggc ctcgcggcgc tggataccat tcgcctgacc agcagcgacg gtggcgtggt
     9181 gccgttaagc tcaattgcca aaattgagca gcgttttgcg ccgctttcca tcaaccatct
     9241 ggatcagttc ccggtaacaa ccatctcctt taacgtgccg gataactatt cgctgggcga
     9301 tgcggtgcag gcgattatgg acaccgaaaa gacgctgaat ctgccggtgg atatcaccac
     9361 gcaattctag ggcagcaccc tcgccttcca gtcggcgctg ggcagcactg tctggctgat
     9421 tgtcgcggcg gtggtggcga tgtatatcgt gctcggcatt ctgtacgaga gctttattca
     9481 cccgatcacc attctctcga cgctacccac cgcaggggtc ggcgcgctgc tggcattaat
     9541 gattgccggt agcgaactgg atgtgattgc gattatcggc attattttgc tgatcggtat
     9601 tgtgaagaag aacgccatca tgatgatcga cttcgcgctg gctgctgagc gcgagcaagg
     9661 catgtcgccg cgcgatgcaa tctaccaggc ttgtctgttg cgttttcgtc cgatcctgat
     9721 gaccaccctg gcggctctgc ttggcgcgct gccgctgatg ttgagtaccg gggtcggcgc
     9781 ggaactgcgt cgtccgttag gtatcggcat ggtcggcggt ctgattgtca gccaggtgct
     9841 gacgctgttc accacgccgg tgatttatct gctgttcgac cgcctggcat tgtggaccaa
     9901 aagtcgcttt gctcgccatg aagaggaggc gtaagtgaag ttttttgccc tcttcattta
     9961 ccgcccggtg gcgacgattt tactgtcggt cgccattacc ctgtgcggca tactgggttt
    10021 ccgtatgctg ccggtcgccc cgctgccgca ggtcgatttt ccggtgatta tggtcagcgc
    10081 ctcgctgccc ggtgcgtcgc cggagacaat ggcgtcttcc gttgccacgc cgctggagcg
    10141 ctcacttggg cgcattgccg gggttagtga aatgacctcc agcagttcgc tcggcagcac
    10201 gcgcattatt ttgcagtttg attttgaccg ggatatcaac ggcgcagcgc gtgatgtgca
    10261 ggcggcgatc aacgctgcac aaagtttgct gcccagcggg atgcccagcc gcccgaccta
    10321 tcgcaaagcg aacccgtcgg atgcgccaat tatgatcctc acgctgacat ccgatactta
    10381 ttcgcagggt gaactgtacg atttcgcctc gacgcagctg gctccgacga tttcgcaaat
    10441 cgacggtgtc ggtgatgtcg atgtcggtgg cagctcgctg cccgccgtgc gcgtcgggct
    10501 gaccccgcag gcgctgttta atcagggcgt gtcgctggac gacgtacgca ctgccatcag
    10561 caatgccaac gtgcgtaaac cgcagggggc gctggaagat ggcactcacc gctggcagat
    10621 ccagaccaac gatgaactga aaaccgccgc tgaatatcag ccgctgatta ttcactacaa
    10681 caacggcggc gcggttcgtc tgggcgatgt ggcgacggtg actgactcag tgcaggatgt
    10741 gcgcaacgcc gggatgacca acgccaaacc ggctatttta ctgatgatcc gcaaactgcc
    10801 ggaagccaat attatccaga cggtagacag catccgggca aaattaccag agttgcagga
    10861 gaccattccg gcggcgattg atctgcaaat tgcccaggat cgctccccca ccattcgcgc
    10921 ctcgctggaa gaagtcgagc aaacgctgat tatctcggtg gcgctggtga ttctggtggt
    10981 ctttttattc ctgcgctcgg gtcgcgccac tattattccc gccgttgcgg tgccggtttc
    11041 gctgattggt acgtttgcgg cgatgtacct gtgcggattc agtctcaata atctttcgct
    11101 gatggcgctc accattgcta ctggtttcgt ggtggatgac gccattgtgg tgctggaaaa
    11161 cattgcccgt catctggaag cggggatgaa accactacag gccgcgctgc aaggtactcg
    11221 cgaagtcggc tttacggtgc tgtcgatgag tctgtcactg gtggcggtgt tcctgccgct
    11281 gctgttgatg ggcggattgc cgggccgact gttacgcgag tttgccgtga cgctttctgt
    11341 cgccattggt atttcgttgc tggtttccct gacgctaacg ccaatgatgt gcggctggat
    11401 gctaaaagcc agcaagccgc gtgagcaaaa gcgactgcgt ggttttggcc gcatgttggt
    11461 agccctgcaa caaggctacg gcaagtcgct gaaatgggtg ctcaatcata cccgtctggt
    11521 gggcgtggtg ctgcttggca ccattgcgct gaatatctcg atcccgaaaa ccttcttccc
    11581 ggagcaggac accggcgtac tgatgggtgg gatacaggcg gatcagagta tttcgtttca
    11641 ggcgatgcgc ggcaaattgc aggatttcat gaaaattatc cgtgacgatc cggcagtgga
    11701 taatgtcacc ggctttaccg gcggttcacg agttaacagt gggatgatgt ttatcaccct
    11761 caagccacgc gacgaacgca gcgaaacggc gcagcaaatt atcgaccgtc tgcgcgtgaa
    11821 gctggcgaaa gaaccggggg cgaatctgtt cctgatggcg gtacaagata ttcgcgttgg
    11881 tgggcgtcag tcgaacgcca gctaccagta cacgttgtta tccgacgacc tggcggcact
    11941 gcgcgaatgg gagccgaaaa tccgcaaaaa actggcgacg ttgccggaac tggcggacgt
    12001 gaactccgat cagcaggata acggcgcgga gatgaatctg gtttacgacc gcgacaccat
    12061 ggcacggctg ggaatcgacg tacaggccgc caacagtctg ttaaataacg ccttcggtca
    12121 gcggcaaatc tcgaccattt accagccgat gaaccagtat aaagtggtga tggaagtgga
    12181 tccgcgctat acccaggaca tcagtgcgct ggaaaaaatg ttcgttatca ataacgaagg
    12241 caaagcgatc ccgctgtcgt atttcgctaa atggcaaccg gcgaatgccc cactatcggt
    12301 gaatcatcag ggattatcgg cggcctcgac catctcgttc aacctgccga ccggaaaatc
    12361 gctctcggac gccagtgcgg cgatcgatcg cgcaatgacc cagcttggtg tgccttcgac
    12421 ggtgcgcggc agttttgccg gcacggcgca ggttttccag gagacgatga actcgcaggt
    12481 gatcctgatt atcgccgcca tcgccacggt gtatatcgtg ctgggtatcc tttacgagag
    12541 ttacgtacat ccgctgacga ttctctccac cctgccctcg gcgggcgttg gagcgctgtt
    12601 ggcgctggag ctgttcaatg ccccgttcag cctaatcgcc ctgataggga tcatgctatt
    12661 aatcggcatc gtgaagaaaa acgccattat gatggtcgat tttgcgcttg aagcccaacg
    12721 gcacggtaac ctgacgccgc aggaagctat tttccaggcc tgtctgctgc gttttcgccc
    12781 gattatgatg accaccctgg cggcgctgtt tggcgcgctg ccgctggtat tgtcgggcgg
    12841 tgacggctcg gagctgcggc aacccctgga gatcaccatt gtcggcggtc tggtgatgag
    12901 ccagctcctg acgctgtata ccacgccggt ggtgtatctc tttttcgacc gtctgcggct
    12961 gcgtttttcg cgtaaaccta aacaaacggt aaccgagtaa atgacagatc ttcccgacag
    13021 cacccgctgg caattatgga ttgtggcttt cggcttcttt atgcagtcgc tggacaccac
    13081 catcgtaaac accgcccttc cctcaatggc gcaaagcctc ggggaaagtc cgttgcatat
    13141 gcacatggtc attgtctctt atgtgctgac cgtggcggtg atgctgcccg ccagcggctg
    13201 gctggcggac aaagtcggcg tgcgcaatat tttctttacc gccatcgtgc tgtttactct
    13261 cggttcactg ttttgcgcgc tttccggcac gctgaacgaa ctgttgctgg cacgcgcgtt
    13321 acagggcgtt ggcggtgcga tgatggtgcc ggtcggcagg ttgacggtga tgaaaatcgt
    13381 accgcgcgag caatatatgg cggcgatgac ctttgtcacg ttacccggtc aggtcggtcc
    13441 gctgctcggt ccggcgctcg gcggtctgct ggtagaatac gcgtcgtggc actggatctt
    13501 tttgatcaac attccggtgg ggattatcgg tgcgatcgcc acattgatgt taatgccgaa
    13561 ctacaccatg cagacgcggc gctttgatct ttccggcttt ttattgctgg cggttggcat
    13621 ggcggtatta accctggcgc tggacggcag taaaggtaca ggtttatcgc cgctggcgat
    13681 tgctggcctg gtcgcagttg gcgtggtggc actggtgctt tatctgctgc acgccagaaa
    13741 taacaaccgc gccctgttca gtctgaaact gttccgcact cgtacctttt cgctgggcct
    13801 ggcggggagc tttgccggac gtattggcag tggcatgttg ccctttatga caccggtttt
    13861 cctgcaaatt ggcctcggtt tctcgccgtt tcatgccgga ctgatgatga tcccgatggt
    13921 gcttggcagc atgggaatga agcgaattgt ggtacaggtg gtgaatcgct ttggttatcg
    13981 tcgggtactg gtagcgacca cgctgggtct gtcgctggtc accctgttgt ttatgactac
    14041 cgctctgttg ggctggtact acgttttgcc gttcgtcctg tttttacaag ggatggtcaa
    14101 ctcgacccgt ttctcctcca tgaacaccct gacgctgaaa gatctcccgg acaatctggc
    14161 gagcagcgga aacagcctgc tgtcgatgat tatgcaattg tcgatgagta tcggcgtcac
    14221 catcgccggg ctgttgctgg ggctttttgg ttcgcagcat atcagcgtcg acagcggcac
    14281 cacacaaacc gtctttatgt acacctggct tagcatggcg tttatcatcg cccttccggc
    14341 gttcatcttt gccagagtgc cgaacgatac gcatcaaaat gtagctattt cgcggcgaaa
    14401 aaggagcgcg caatgaagtt ctggcgaccc ggtattaccg gcaaactgtt tctggcgatt
    14461 ttcgccacct gcattgtctt gctgatcagt atgcactggg cggtgcgtat cagttttgag
    14521 cgtggcttta ttgattacat caaacacggc aatgaacagc gattacagct gttaagtgat
    14581 gcgcttggcg agcagtatgc gcagcatggt aactggcgct tcctgcgcaa caacgatcgc
    14641 tttgtctttc agatcctgcg ttcatttgaa cacgataatt cggaagataa acccggcccg
    14701 ggtatgccgc cgcacggctg gcgtacccag ttctgggtgg tcgatcaaaa caacaaagta
    14761 ctggttggtc cgcgagcgcc gattccacct gacggtacac ggcgacccat tctggtcaac
    14821 ggtgcggaag tgggcgcggt gattgcctcc cctgttgagc ggctgacccg caatactgat
    14881 atcaatttcg ataaacaaca gcggcaaacc agctggctga tcgtcgccct ggcaacgtta
    14941 ctcgcggcgc tcgccacctt tctgctggcg cgcggtttgc tggcaccggt aaaacgactt
    15001 gtcgatggca cgcacaaact ggcggcgggc gatttcacta cccgcgtaac gcccaccagt
    15061 gaagatgaac tgggcaaact ggcgcaagac ttcaaccagc tcgccagcac gctggagaaa
    15121 aaccagcaaa tgcggcgcga ttttatggcc gatatttctc acgaactgcg tacgccatta
    15181 gcggtgctgc gcggtgaact ggaagccatt caggatggcg tgcgcaaatt cacgccggag
    15241 acggtggcgt ctttacaggc ggaggtcggt acactgacca aactggttga cgatcttcat
    15301 cagttgtcga tgtctgatga aggcgctctc gcctatcaaa aagcaccggt agatttgatc
    15361 ccactgctgg aagtggcggg cggcgcattt cgcgaacgat tcgccagccg tggcctgaaa
    15421 ctgcaatttt ccctgccaga cagtatcacc gtatttggcg atcgcgaccg tttaatgcag
    15481 ttattcaata acttactgga aaacagcctg cgctacactg acagcggcgg cagcctgaaa
    15541 atctctgccg agcagcacga caaaacggtg cgcctgacct ttgccgacag tgcgccaggt
    15601 gtcagtgacg atcagctaca aaaattgttt gaacgttttt atcgcaccga aggttcccgc
    15661 aaccgcgcca gcggcggttc cgggctgggg ctggcgattt gcctgaacat tgttgaagcc
    15721 cataatggcc gcattattgc cgcccattcg ccttttggcg gggtaagcat tacagtagag
    15781 ttaccgctgg aacgggattt acagagagaa gtatgaccga gttaccaatc gacgaaaaca
    15841 caccgcgtat tttgatcgtg gaagatgaac cgaagctggg gcagttgctc attgattatc
    15901 tgcgtgctgc gagctatgcg ccgacgctta tcagccacgg cgatcaggta ctggcgtatg
    15961 tgcgccagac accaccggat ctgatcctgt tggatctgat gctccctggc accgatggcc
    16021 tgacattatg ccgggaaatt cgtcgttttt ccgatgttcc gatcgtgatg gtgacggcaa
    16081 aaatcgaaga gatcgaccgc ctgctggggc tggagattgg cgccgatgac tacatctgca
    16141 agccgtacag cccacgggaa gtggtagcgc gtgtcaaaac cattttgcgc cgttgtaaac
    16201 cgcaacgcga acttcagcaa caggatgccg aaagcccgtt gattatcgac gaaggtcgtt
    16261 ttcaggcttc atggcgcggt aaaatgcttg acctgacgcc tgcggaattt cgtctgctta
    16321 aaacgctctc tcacgaacca ggaaaagtgt tctcccgcga gcaattgctc aatcatcttt
    16381 atgacgacta ccgcgtagta accgaccgca ccattgacag ccacattaaa aatctgcgcc
    16441 gcaagctgga atctctcgac gccgaacagt catttatccg cgccgtttac ggcgttggtt
    16501 accgctggga agccgacgcc tgccgcatcg tttagtttta gcgacattac tttgttagcc
    16561 ggagatggcg ttcagacccg atccggcaac attattccac gcatggtcag caaactgaca
    16621 ttatctctcc gccctggcag gcatcagaaa atcgctcata ctttaatcag taaacagcac
    16681 ctttagatgc tgttttcgat aagcaatttc aatcaaggag tcattatggc tggttggttt
    16741 gaactcagta agagcagtga taatcagttc cggtttgtgc taaaagcggg caatggtgag
    16801 actatcctca ccagcgagct gtatacctca aaagcctctg cggaaaaggg catcgcgtcg
    16861 gtgcgtagca acagcccgca agaagagcgc tatgagaaaa aaacggcaag caacggcaaa
    16921 ttctatttca atctgaaagc cgctaatcat caaattatcg gctccagcca gatgtacgcc
    16981 accgcgcaat ctcgtgaaac cggaattgcc tccgttaaag ccaatggcac aagccagacg
    17041 gtgaaagaca atacgtaatt acgatgccgg gcgtgggttg ttggcgtccg gcgctttacc
    17101 agatcccccc tacagcgcta caatgcccgc ccttaaagtg ggggcctccc ctaaccgctt
    17161 catcaggtga agcggatctg acctgtcatc agaacgagag aattatgttt aaaccggaac
    17221 tcctttcccc ggcgggaacg ctgaaaaata tgcgttacgc tttcgcttat ggcgcagatg
    17281 ctgtttatgc gggccagccg cgttactccc tacgtgtgcg caacaacgaa ttcaaccacg
    17341 aaaatcttca gctcggcatc aatgaagccc acgcgctggg gaaaaagttt tatgtcgtgg
    17401 tcaacattgc accgcacaac gccaagctga aaacctttat ccgtgacctg aaaccggtgg
    17461 tggaaatggg gccggatgcg ctgattatgt ccgatccagg gctgattatg ctggtgcgtg
    17521 agcacttccc tgaaatgccg atccacctct cggtgcaggc taacgccgtg aactgggcga
    17581 cggtgaaatt ctggcagcaa atgggactga cccgcgtgat cctctctcgc gagctgtcgc
    17641 tggaagagat tgaagagatc cataatcagg tgccggatat ggagatcgag atcttcgttc
    17701 acggcgcact gtgcatggcc tactccggtc gctgcctgct ctctggctat atcaacaagc
    17761 gcgacccgaa ccagggcacc tgcaccaacg cctgccgctg ggagtacaac gtccaggaag
    17821 ggaaagaaga tgacgttggc aacatcgtac acaagtacga gccgattccg gtgcaaaatg
    17881 ttgagccgac gctgggtatc ggcgcgccaa ccgacaaagt gtttatgatc gaagaagccc
    17941 agcgtccggg cgagtatatg accgcgtttg aagatgagca cggcacttac atcatgaact
    18001 cgaaagatct gcgcgccatc gcccatgtag aacgcctgac caaaatgggc gtgcattcgc
    18061 tgaaaatcga aggtcgtacc aaatctttct actattgcgc acgcaccgca caggtttacc
    18121 gcaaagctat cgatgacgcc gctgcgggaa aaccgttcga taccagcctg ctggaaactc
    18181 tggaaggtct ggcgcatcgt ggctataccg aaggtttcct gcgtcgtcat actcacgacg
    18241 attatcagaa ctacgaatac ggttattcag tttctgaccg ccagcagttt gttggtgagt
    18301 ttaccggtga gcgcaagggg gacctcgcag cggtagcggt gaaaaataaa ttctccgttg
    18361 gcgacagtct tgagctgatg acgccgcaag gcaacattaa ctttaccctt gagcacatgg
    18421 aaaacgccaa aggcgaagct atgccggtcg caccaggcga tggttatact gtgtggctcc
    18481 cggtcccgca ggatcttgag ctaaattacg cgctgctaat gcgtaatttc tccggggaaa
    18541 ccacgcgtaa cccccacggt aagtgattaa tttcgattat ttttcccgga tggaaaattc
    18601 ttagaaaccg atcacataca gctgcattta ttaaggttat catccgtttc gctgaaaaac
    18661 ataatccata aaatgctagc tgtaccagga accacctcct tagcctgtgt aatctccctt
    18721 acacgggctt attttttgta cataagctgt aaagaaggat tgatccctga taaccgtctt
    18781 ccattgacca catcgataga atcccccttc atagcacaat gcctttcacg taacggtatc
    18841 gtgctcgccc aggttccggc tacgcacagc cagaacgcgc atgtttgacg cttacaaaaa
    18901 aatattctca ctctccacat ttgaatgtca gacgagcgac gccatgtaat cctgcacctt
    18961 ctgtcttcag gtcaactatc tgcatttttt tgccctgagt aacacagaaa tgggctgcat
    19021 cattttttac tatattttct gcaccagata ttctgcccct ggctaaagaa gcttcggctt
    19081 cggtgtagta ttggttatcg agtttacgct gaatattact tttatatgca agaccaaatt
    19141 taccgatact tgtttcatca ttatgcacag cacaaccaga tataagaaaa atactaatta
    19201 atgatatagc agctatcttt ttcatctcac
//
LOCUS       DAZSNM010000066        18691 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZSNM010000066 DAZSNM010000000
VERSION     DAZSNM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18691
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.066"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            225..1591
                     /gene="mqo"
                     /locus_tag="ACHW42_001909"
                     /pseudo
     CDS             225..1591
                     /gene="mqo"
                     /locus_tag="ACHW42_001909"
                     /inference="COORDINATES: protein motif:HMM:NF017817.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
     gene            complement(1713..2222)
                     /gene="eco"
                     /locus_tag="ACHW42_001910"
     CDS             complement(1713..2222)
                     /gene="eco"
                     /locus_tag="ACHW42_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR8943746.1"
                     /translation="MKKESKKMKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     TGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAEVR"
     gene            complement(2336..2500)
                     /gene="yojO"
                     /locus_tag="ACHW42_001911"
     CDS             complement(2336..2500)
                     /gene="yojO"
                     /locus_tag="ACHW42_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR8943747.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            2609..3103
                     /gene="napF"
                     /locus_tag="ACHW42_001912"
     CDS             2609..3103
                     /gene="napF"
                     /locus_tag="ACHW42_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR8943748.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            3093..3356
                     /gene="napD"
                     /locus_tag="ACHW42_001913"
     CDS             3093..3356
                     /gene="napD"
                     /locus_tag="ACHW42_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR8943749.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            3353..5839
                     /gene="napA"
                     /locus_tag="ACHW42_001914"
     CDS             3353..5839
                     /gene="napA"
                     /locus_tag="ACHW42_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR8943750.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            5846..6541
                     /gene="napG"
                     /locus_tag="ACHW42_001915"
     CDS             5846..6541
                     /gene="napG"
                     /locus_tag="ACHW42_001915"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR8943751.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            6528..7391
                     /gene="napH"
                     /locus_tag="ACHW42_001916"
     CDS             6528..7391
                     /gene="napH"
                     /locus_tag="ACHW42_001916"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR8943752.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            7388..7837
                     /gene="napB"
                     /locus_tag="ACHW42_001917"
     CDS             7388..7837
                     /gene="napB"
                     /locus_tag="ACHW42_001917"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR8943753.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            7847..8449
                     /gene="napC"
                     /locus_tag="ACHW42_001918"
     CDS             7847..8449
                     /gene="napC"
                     /locus_tag="ACHW42_001918"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR8943754.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            8462..9085
                     /gene="ccmA"
                     /locus_tag="ACHW42_001919"
     CDS             8462..9085
                     /gene="ccmA"
                     /locus_tag="ACHW42_001919"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR8943755.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            9082..9744
                     /gene="ccmB"
                     /locus_tag="ACHW42_001920"
     CDS             9082..9744
                     /gene="ccmB"
                     /locus_tag="ACHW42_001920"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR8943756.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            9786..10523
                     /gene="ccmC"
                     /locus_tag="ACHW42_001921"
     CDS             9786..10523
                     /gene="ccmC"
                     /locus_tag="ACHW42_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR8943757.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            10520..10729
                     /gene="ccmD"
                     /locus_tag="ACHW42_001922"
     CDS             10520..10729
                     /gene="ccmD"
                     /locus_tag="ACHW42_001922"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR8943758.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            10726..11205
                     /gene="ccmE"
                     /locus_tag="ACHW42_001923"
     CDS             10726..11205
                     /gene="ccmE"
                     /locus_tag="ACHW42_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR8943759.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            11202..13145
                     /gene="ccmF"
                     /locus_tag="ACHW42_001924"
     CDS             11202..13145
                     /gene="ccmF"
                     /locus_tag="ACHW42_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR8943760.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            13142..13699
                     /gene="dsbE"
                     /locus_tag="ACHW42_001925"
     CDS             13142..13699
                     /gene="dsbE"
                     /locus_tag="ACHW42_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR8943761.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            13696..14748
                     /gene="ccmH"
                     /locus_tag="ACHW42_001926"
     CDS             13696..14748
                     /gene="ccmH"
                     /locus_tag="ACHW42_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR8943762.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(14959..15606)
                     /gene="narP"
                     /locus_tag="ACHW42_001927"
     CDS             complement(14959..15606)
                     /gene="narP"
                     /locus_tag="ACHW42_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR8943763.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            15926..18517
                     /gene="yejO"
                     /locus_tag="ACHW42_001928"
     CDS             15926..18517
                     /gene="yejO"
                     /locus_tag="ACHW42_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFR8943764.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
BASE COUNT         4190 a         4572 c         5405 g         4524 t
ORIGIN      
        1 ccgtggggct taatgccgtt tcgatggcgg caaaagcgaa agcgtccgag gagcaggaaa
       61 ctgatgtact gttgattggc ggcggcatta tgagcgccac gttggggacc tattgacgcg
      121 agctggagcc tgaatggtcg atgaccatgg tggagcgcct ggaggggggc gcgcaggaga
      181 gttcgaacgg ctagaacaac gccggaaccg ggcattctgc actgatggaa ctgaactaca
      241 ccccgcaaaa cgccgatggc agcatcagta ttgaaaaagc agtcgccatt aacgaagcat
      301 ttcagatttc ccgccagttc tgggcgcacc aggtcgagcg cggcgtgctg cgtactccgc
      361 gttcatttat caataccgtt ccgcatatga gctttgtctg gggcgaggat aacgttaatt
      421 tcctgcgcgc ccgttacgcc gcgttgcaac aaagctcgct gtttcgcggt atgcgttact
      481 ctgaagatca cgcgcagatc aaagagtggg caccgttagt gatggaaggg cgcgatccgc
      541 aacagaaatt ggcactgatg tgaactatgg tgagatcacc cgccagttaa ttgcttcctt
      601 acagaagaaa tctaacttct cgctgcaact cagcagcgaa gtccgcgccc taaagcgtaa
      661 tgacgataac acctggaccg ttaccgttgc cgatctgaaa aacggcactg cacagaacat
      721 ccgcgccaaa tttgtcttta tcggcgcggg cggtgcggcg ctgaagctgt tacaggaatc
      781 ggggattccg gaagcgaaag actacgccgg tttcccggtg ggcggacagt tccttgtttc
      841 ggaaaatcca gacgtggtta atcgccatct ggcgaaagtt tacggtaaag catccgttgg
      901 cgcaccaccg atgtcggttc cgcatatcga tacccgtgtt ctggacggta aacgcgtagt
      961 gctgtttggg ccatttgcca ccttctcaac caaattcctc aaaaacggtt cattgtggga
     1021 tctgatgagt tccaccacca cctctaacgt gatgccgatg atgcacgtcg ggctggataa
     1081 tttcgatctg gtgaaatatc tggtgagtca ggtgatgttg agtgaagagg atcgttttga
     1141 agcgttgaaa gagtactatc cgcaagcgaa aaaagaggac tggcgtttgt ggcaagcggg
     1201 gcagcgtgtg cagattatca agcgtgatgc cgagaaaggt ggcgtactgc gtctgggtac
     1261 cgaagtggtc agtgaccagc aagggaccat tgcggcgctg ctgggggcat cgccaggggc
     1321 gtcaaccgcc gcgccgatta tgttgaatct actggaaaaa gtatttggcg atcgcgtttc
     1381 cagcccgcaa tggcaggcta cgttgaaagc gatcgttccg tcttatggac gcaagctgaa
     1441 cggtgatgta gcggcaacag aacgcgagtt gcagtacacc agcgaagtgc tggggctgaa
     1501 atacgacaaa ccgcaagcag cagatagtac gccgaaaccg cagttgaaac cgcaacccgt
     1561 tcaaaaagaa gtggcggata ttgcgttgta atgatgtggc gtatactgat aagacgcgcc
     1621 agcgtcgcat caggcaaccg gctcgggcgt tagatgttgg atgcggcgta accgccttat
     1681 ccaaccaacg gggtgccgta cttcacaaca gtttagcgaa cttccgcgtt gtcaattttc
     1741 tcttccgcct tccagacgcg gtatttcaca tctacattgt ctggcgtata caccacgatc
     1801 ggcagcttgc tgttgtaacg cagcattcca gcatcgccca gatacgcggt gacaaatttc
     1861 ttctctttct tgccatccgg gcaggccatc atcgtggaaa ccggcgagct aactttatca
     1921 aagacataat aatcatagcc ccagccttcc agcgttttgc tttccagctt cccgccgaga
     1981 cgatgcaaat tgcaatcgac ttccagcgtc tgaccgatta acagttctac tttcagggta
     2041 gattcatctt cttgcggggt taactgaatc acctgacgct tcatgcctgt ttcagcttgt
     2101 ggataaggcg cgattttttc cagtggctgg acgctttctg ccgcccaggc ggaagtggta
     2161 gcgaaagcgg caaacaatac ttcaggtaga atggtcttca tttttttgct ttccttcttc
     2221 atttataggt tttcaccgat gatgtcgctg aaggttaaca tattcatcag atttattaat
     2281 ttgctttatg aataacttaa agaacattca gacaatttta cctgctcttt tagtgttaaa
     2341 ttctgatgag ggagcaaacc agcagaaata tctctggtga attaatcgct atataaatat
     2401 gtttataacc atttgaaatg tgagcaaaag cccgtttttg ccacgcttcc cgccactctt
     2461 ttgatcctgc tacaggtttt accccgatcg gggtatgcat ctttgacaca tcctttaata
     2521 tcttagcggc tataaaaatg gcttattaat tatgcggtct atttggtcgc tctcaatttt
     2581 cagagcgcgt taatgatgga aggtcaatgt gaagattgat gcatcccgtc ggggcatact
     2641 cactggttgc tggcgcaaag ccagtaacgg tatccgtccg ccctggtccg gtgatgaatc
     2701 tcattttctg acccattgca cccgctgcga cgcctgtatt aatgcttgtg aaaacaacat
     2761 tctgcaacgc ggcacgggtg gctatccgag cgttaatttc aaaaataacg agtgcagctt
     2821 ctgttacgcc tgcgcgcagg cttgccccga atcgctattt tctccgcgcc acaccagggc
     2881 ctgggatttg cagttcacca tcggggacgc gtgcctggcg tatcagtcag ttgaatgccg
     2941 ccgctgtcag gatagctgtg agccaatggc aattatcttt cgcccgacgc tctccgggat
     3001 ctaccaggcg caacttaata gccaactttg caacggatgt ggagcatgtg cagccagctg
     3061 tccggtatca gccataactg cggagtatct ccatgcacac taactggcaa gtttgcagcc
     3121 tggtcgtgca ggccaaaagc gaacgaattt cagacatcag cacccaactg aacgcctttc
     3181 ccggctgtga agttgctgtc agcgacgcgc cgagcggtca gttgattgtg gtggtggaag
     3241 cagaagacag cgaaacgctg atccaaacca ttgagtcagt acgcaacgta gagggcgtgc
     3301 tggcggtgtc gctggtttat caccagcagg aagagcaagg tgaggaaaca ccatgaaact
     3361 cagtcgtcgt agctttatga aagctaacgc cgttgcggcc gctgcggcgg ctgccggtct
     3421 cagcgtgccg ggcgttgccc gcgccgttgt tggtcagcag gaagccatta aatgggataa
     3481 agcgccgtgc cgtttctgcg gtactggttg cggcgttctg gtcggaacgc agcaggggcg
     3541 tgtggtggcc tgtcagggcg acccggacgc accggttaac cgtggcctga actgcattaa
     3601 gggctatttc ctgcccaaga tcatgtacgg taaagaccgt ttgacgcagc cgctgctgcg
     3661 tatgaaaaac ggtaaatatg acaaagaagg cgaatttacc ccaatcacct gggatcaggc
     3721 cttcgatgtg atggaagaga aattcaaaac cgccctgaaa gaaaaagggc cggaatcgat
     3781 cggtatgttc ggttctggtc agtggactat ctgggaaggt tatgccgcgt ccaagctgtt
     3841 caaagcgggc ttccgttcga acaacatcga cccgaacgca cgtcactgta tggcgtcggc
     3901 agtagttggc tttatgcgta cctttggtat ggatgagccg atgggctgct atgacgacat
     3961 cgagcaggct gacgcgtttg tgctgtgggg cgctaacatg gcggagatgc acccgatcct
     4021 ctggtcacgc atcactaaca gtcgtctctc taaccagaac gtcaccgtgg cggtgctttc
     4081 tacctaccag catcgtagct tcgagctggc ggataacggc atcatcttta cgccgcaatc
     4141 tgacctggtg atcctgaact acatcgccaa ctatatcatt caaaacaatg cgataaatca
     4201 ggacttcttc agcaagcacg ttaacctgcg caaaggggcg acggacatcg gctacggttt
     4261 acgtccgacc catccgctgg aaaaagcagc gaagaatccg ggttctgacg cctccgaacc
     4321 gatgagcttt gaagattaca aagccttcgt tgctgagtat acgctggaaa aaaccgctga
     4381 aatgaccggc gtgccgaaag accagttaga acaactggcg cagctgtatg ccgatccgaa
     4441 caagaaagtc atctcctact ggacgatggg cttcaaccag catacccgtg gtgtgtgggc
     4501 taacaacctg gtctacaacc tgcacctgct gaccggcaaa atttcccagc cgggttgcgg
     4561 tccgttctcc ctgaccgggc aaccttctgc gtgtggtact gcgcgtgaag tgggcacctt
     4621 tgctcaccgt ctgcctgcgg acatggtggt aactaacgag aaacatcgtg atatctgtga
     4681 gaagaagtgg aatatcccga gcggcaccat tccggcgaaa atcggtcttc atgcggtggc
     4741 acaagaccgt gcgctgaaag acggcaagct gaatgtttac tggaccatgt gtaccaacaa
     4801 catgcaggcc gggccgaaca tcaatgaaga gcgtatgccg ggctggcgcg atccgcgcaa
     4861 cttcatcatc gtctccgatc cgtatccgac agtcagtgcg ctggccgccg acttgatcct
     4921 gccgaccgca atgtgggtag agaaagaggg cgcttacggt aacgccgaac gccgtactca
     4981 gttctggcgt cagcaggtac aggcgccagg cgaagcgaaa tcggatctct ggcagttagt
     5041 ccagttctcc cgccgcttca aaactgaaga agtatggccg gaagagctgc tggcgaagaa
     5101 accggaactg cgtggcaaaa cgctgtacga agttctgtat gccacccccg aagtgagcaa
     5161 attcccggta tccgaactgg cggaagatca gctgaacgat gaatcccgcg agctgggctt
     5221 ctatctgcaa aaagggctgt tcgaagagta cgcatggttt ggtcgcggtc acggtcacga
     5281 tctggcaccg ttcgatgact accacaaagc gcgcggtctg cgctggccgg tggtgaatgg
     5341 taaagaaaca cagtggcgtt acagcgaagg taacgacccg tacgtgaaag cgggcgaagg
     5401 ctataagttc tacggtaaac cggatggcaa agcggtgatc ttcgcactgc cgttcgaacc
     5461 ggcggcggaa gcaccggatg aagagtacga cctgtggctc tctaccggac gcgttctgga
     5521 gcactggcac accggcagta tgactcgccg tgtgccggaa ctgcaccgcg ccttcccgga
     5581 agcggtcttg tttattcacc cgctggatgc gaaagcgcgc gatctgcgcc gtggcgacaa
     5641 agtgaaagtg gtttctcgcc gtggcgaagt gatctcgatt gttgaaacgc gcggtcgtaa
     5701 ccgtccgcca cagggcctgg tgtacatgcc gttcttcgac gccgcacagc tggttaacaa
     5761 actgacgctg gatgcgaccg atccgctctc gaaagagacg gacttcaaga agtgcgcggt
     5821 caaactggag aaggtgtaag ccattatgtc ccggtcagcg aaacctcaaa atggtcgccg
     5881 ccgctttctg cgcgatgtcg ttcgcacagc aggcgggctg gctgccgtgg gtgtggcgct
     5941 ggggttacaa cagcaaaccg cacgcgcatc tggcgtgcgg ttgcgcccgc ccggagccat
     6001 aaacgagaac gcctttgcca gtgcctgtgt gcgttgcggt cagtgtgttc aggcttgccc
     6061 ttacgacacc ttaaaactgg cgacgctggc ctctggtctg tcggcgggca cgccatattt
     6121 tgtcgcacgg gatattcctt gcgaaatgtg tgaggacatt ccgtgcgcca aagtgtgccc
     6181 aagcggtgcg ctggatcgtg agattgaatc gatcgacgac gcgcggatgg ggctggcggt
     6241 actggtggac caggaaaact gtctcaactt tcaggggctg cgctgcgatg tttgttatcg
     6301 cgaatgcccg aaaattgatg aggccatcac cttggagctg gagcgcaaca cgcgtaccgg
     6361 taagcacgcc cgctttctgc cgacggttca cagcgacgcc tgtaccggtt gcggtaagtg
     6421 cgaaaaagta tgcgtgctgg aacaaccggc aatcaaggtg ttaccgctgt cactggcgaa
     6481 aggggagtta ggtcaccatt accgcttcgg ctggctggag gggaacaatg gcaaatcgta
     6541 aacgtgacgc cgggcgcgag gcgctggaga aaaaaggctg gtggcgcagt caccgttggc
     6601 tggtgttacg tcgcctttgt cagttcttcg tgctggggat gtttttgagc ggtccatggt
     6661 ttggtgtgtg gatcttgcac ggcaactaca gcagtagcct gttattcgac accgttccgc
     6721 tgaccgatcc gctgatgacg ctgcaaagtc ttgccagtgg tcatctgcct gctacggtgg
     6781 cgttgaccgg tgcggtgatt atcaccgtgc tctacgccct ggcgggaaag cgattatttt
     6841 gcagctgggt ctgcccgctg aacccgatta ccgacttagc gaactggtta cgcaggcgct
     6901 ttgacctcaa tcagtctgcg acgatccctc gccacattcg ctacgtgctg ttggtggtca
     6961 ttctggtggg atcagcctta actggcacgc tcatttggga atggatcaac cccgtttctc
     7021 tgatggggcg tagcctggta atgggcttcg gcagcggcgc gctgcttatt ctcgcgctgt
     7081 ttttatttga tctactggtc gttgaacacg gctggtgcgg gcacatttgt ccggttggcg
     7141 cgctgtatgg cgtgctgggt agcaaaggtg tgattaccgt tgcggccagc gatcggcaga
     7201 aatgtaaccg ctgtatggat tgttttcatg tttgcccgga accgcatgtg ctacgtgccc
     7261 cggtgctgga tgaacaaagc ccggtgcagg tcaccagccg cgattgcatg acgtgcggtc
     7321 gctgcgtgga tgtctgttct gaggatgtat ttacaataac tacacgatgg agttcgggag
     7381 cgaaatcatg aaaagccatg acctgaagaa agcgctgtgt caatggacgg cgatgctggc
     7441 cctggtggta agcggcgcgg tttgggcggc taatggcgtc gattttagcc agtcgccgga
     7501 agtatccggg acgcaggaag gggccattcg gatgccaaaa gagcaggatc ggatgccgct
     7561 gaactatgtg aatcagccgc caatgatccc gcatagcgtt gaaggttatc aggtaacgac
     7621 caataccaat cgctgcttgc agtgccacgg tgtcgaaagc tatcgcacca ctggcgcgcc
     7681 gcgtatcagt cctactcact ttatggacag cgacggcaaa gtgggcgcgg aagtggctcc
     7741 gcgtcgttat ttctgtctgc aatgtcacgt accgcaggcc gataccgcgc caatcgtggg
     7801 gaataccttt accccatcaa aaggttacgg gaaataagag gttattatgg gaaattctga
     7861 ccgtaagcct ggtctgatta agcgcctgtg gaaatggtgg cgtaccccca gccatctggc
     7921 gctggggacg ctgctgttga tcggttttgt tggcggcatc gtcttctggg ggggctttaa
     7981 caccgggatg gaaaaagcca ataccgaaga gttctgcatt agctgccacg aaatgcgcaa
     8041 cacggtgtat caggaataca tggattccgt gcactacaac aaccgtagcg gcgtccgtgc
     8101 gacctgtccg gattgtcacg ttccgcacga gtttgtgccg aagatgatac gcaagctcaa
     8161 agcaagtaaa gagctgtatg gtaaaatttt tggcgttatt gacacgccgc agaaatttga
     8221 agctcatcgt ctgacgatgg cacagaatga gtggcggcgc atgaaggaca ataactcgca
     8281 ggagtgccgt aactgtcaca acttcgagta tatggataca accgcccaga aatcggttgc
     8341 cgcgaagatg catgaccagg cggtgaaaga tgggcaaacc tgtattgatt gccataaagg
     8401 gatcgcgcac aagctgcccg atatgcgtga agtcgagcca ggtttttaac agggttattg
     8461 cgtgggtatg cttgaagcca gagagttact ttgtgagcgg gatgaacgaa ccttatttag
     8521 tggcttgtca tttacgctga acgcaggaga gtgggtacaa atcaccggta gcaacggcgc
     8581 ggggaagaca acgcttctcc gtttgctgac ggggttgtct cgccctgacg ctggcgaggt
     8641 tctctggcaa gggcagccct tgcatcaggt acgcgacagc taccatcaaa ccctgttatg
     8701 gataggccat cagccgggga tcaaaacccg gctgacggcg ttagaaaatc tgcactttta
     8761 tcatcgcgat ggcgatgccg cacaatgtct ggaagccttg gcgcaggccg ggcttgccgg
     8821 attcgaagat attccggtaa atcaactttc tgccgggcaa caacgccgcg tcgctttagc
     8881 gcgtctgtgg ttgacccgtg ccacattatg gatcctcgac gagcctttta ccgcgattga
     8941 cgttaacggc gtcgatcgtc tgacccagcg tatggcgcag catacggagc agggggggat
     9001 tgtgattctg actacccacc agccgctcaa cgttgctgaa agtaaaattc gccgcatttc
     9061 actgacgcaa acgagggccg catgatgttc tggcgtattt tccgtcttga actgcgcgta
     9121 gcgtttcgcc atagcgccga aatcgccaac ccgctgtggt tcttcctgat tgtaattacc
     9181 ctttttccgc tcagtatcgg tccggagccg caactgctgg cgcgtattgc accgggcatt
     9241 atctgggttg ctgcgctgct ttcatccttg ctggcgctgg aacgactgtt ccgtgacgat
     9301 ttgcaggacg gcagtcttga acaattgatg ttgttgccgt tacccttgcc cgccgttgtg
     9361 ctggcgaagg tgatggcgca ctggatggta accggtctgc cgttactcat cctttcgcca
     9421 ctggtagcaa tgctactggg aatggatgtt tatggctggc aagtgatggc gctgacgctg
     9481 ctgctgggaa cgcctacgct tggctttctc ggtgcaccgg gcgtggcgct gacagtggga
     9541 cttaagcgcg gtggtgtgct gctcagcata ctggtgttac cgctgactat cccattactc
     9601 atctttgcca ccgccgcgat ggacgcggct tctatgcatt tgcccgttga tgggtatctg
     9661 gcaattttag gcgcgttgct ggcaggcacc gcgacattaa gtccttttgc gacggcggca
     9721 gcgttacgaa tcagcattca ataacgctgg attactgatt tttgttcgag tctggtatcg
     9781 aaactatgtg gaaaacactg catcaactgg cgatcccacc acggctgtat caaatctgtg
     9841 gctggtttat accgtggctg gcaattgcca gtgtggtcgt gcttaccgtc ggctggatct
     9901 ggggattcgg ctttgctccg gctgattatc agcagggaaa tagctaccgc attatctacc
     9961 tgcatgtgcc tgcggcgatc tggtcgatgg gcatttatgc atcaatggcg gtggcggcgt
    10021 ttattggcct tgtctggcag atgaaaatgg ccaacctggc ggtggcggcg atggccccca
    10081 ttggtgccgt gtttaccttt attgccctgg ttaccggctc tgcatgggga aaaccgatgt
    10141 ggggcacctg gtgggtatgg gatgcacgtc tgacttctga actggtgctg ctgtttttgt
    10201 atgtgggtgt gattgccctg tggcacgcct tcgacgaccg ccgtctggcg ggccgtgcgg
    10261 caggtatcat ggtgctgatt ggcgtggtga atctgccgat tattcattac tccgtggagt
    10321 ggtggaacac cctgcatcag ggatcaacgc ggatgcagca aagtatcgat ccggcgatgc
    10381 gttcgccgct gcgctggtcg atttttggct tcctgctcct gtctgccacg ctgacgctga
    10441 tgcggatgcg taatttgatt ttgctgatgg aaaaacgccg tccgtgggtg agtgaactga
    10501 tactgaaaag aggccgtaaa tgacccctgc atttgcttcc tggaatgaat ttttcgcaat
    10561 gggcggttac gccttttttg tctggctggc ggtggtgatg accgttattc cgctggtggt
    10621 tttggtcgtg cactcggtga tgcaacatcg cgcaattctg cgtggtgtgg cgcaacagcg
    10681 ggcgcgtgag gcgcgtttac gtgctgcgca acagcaggag gctgcatgaa tattcgccgt
    10741 aaaaaccgct tgtggattgc ctgtgccgtg ttggcagggc tagcgctgac tatcggtctg
    10801 gtgctatatg cgctgcgctc gaatatcgat ctcttttata cgccggggga aattctctac
    10861 ggcaagcgtg aaactcagca gatgccggaa gtcggtcagc gtctgcgcgt tggcgggatg
    10921 gtgatgccgg gtagcgtgca gcgcgatccc aattcgctga aagtgacctt caccatttac
    10981 gatgctgaag gctcagtgga tgtctcttac gaaggcattt tgccggatct gttccgtgaa
    11041 gggcagggcg ttgtggtgca gggcgagctg gaaaaaggca atcatatcct cgcgaaagaa
    11101 gtgctggcga aacacgacga aaactacacg ccgccagaag ttgagaaagc gatggaagct
    11161 aaccaccgtc gcccggcgag tgtttataag gacccagcat catgatgccg gaaattggaa
    11221 acgggctgct gtgcttggcg ttagggattg cgctgctgct gtccgtgtat ccgctatggg
    11281 gcgtggcgcg cggagatgcg cgcatgatgg cgtcttcccg cttgtttgcc tggctgctgt
    11341 ttatgtctgt ggctggcgca tttctggtgc tggtcaatgc tttcgtggtc aacgacttta
    11401 ccgtcaccta tgttgccagc aactccaata cccagcttcc ggtgtggtat cgcgtggcgg
    11461 ctacttgggg cgcgcatgaa ggttcgcttc tgctgtgggt gctgctgatg agcggctgga
    11521 cctttgcggt ggcgattttt agtcagcgta ttccgctgga tattgtggcc cgtgtactgg
    11581 cgataatggg gatggtcagc gtcggctttt tgctgttcat tctctttacc tctaacccgt
    11641 tctcacgcac gctgccgaac ttcccgattg aagggcgcga tcttaacccg ctgttacagg
    11701 atccggggct gatcttccat ccgcctctgc tttatatggg gtacgtgggt ttctcggtgg
    11761 cgtttgcttt tgccattgct tctttgctga gcgggcgtct ggacagcact tatgcgcgtt
    11821 ttactcgccc gtggacgctg gcggcgtgga ttttcctaac gctcggcatt gtgctcggtt
    11881 ccgcatgggc ctattacgaa ctcggctggg gtggctggtg gttctgggat ccggtagaaa
    11941 acgcctcgtt tatgccgtgg ctggtgggga ctgcactgat gcactcactg gcggtcactg
    12001 aacaacgcgc cagcttcaaa gcgtggacat tactgctggc aatcagtgcc ttctcgttgt
    12061 gtctgttggg gactttcctc gtgcgttccg gcgtgctggt atcggtacac gcgtttgcgt
    12121 ctgatccggc gcgcggtatg tttatcctcg cctttatggt gctggtgatt ggcggttcgc
    12181 tgctgctgtt tgccgcgcgt ggacacaaag ttcgctcacg cgtaaacaat gcgctgtggt
    12241 cgcgggaatc tttgctgtta gcgaacaacg ttttgctggt cgccgcgatg ctggtggtgt
    12301 tgctggggac gctgctgccg ctggtgcaca agcaacttgg actgggcagt atttcgattg
    12361 gcgaaccgtt cttcaacacc atgtttacct ggctgatggt gccgtttgcg ctactgcttg
    12421 gtgtcggtcc tctggtgcgc tgggggcgcg atcgcccacg taaaatccgc aatttattga
    12481 ttatcgcctt catctctacg ctggtgctgt ctctgctgtt gccgtggctg ttcgaaagca
    12541 aagttgtggc gatgacggtg ctcggcctgg caatggcctg ctggattgcg gtgctggcaa
    12601 ctgcggaagc tgcgctgcgt atttcacgcg gcacgaaaac caccttcagt tattggggga
    12661 tggtggcggc tcacctgggg ctggcagtga caattgttgg cattgccttt agccagaatt
    12721 atagcgttga gcgtgatgtg cgcatgaagt ccggcgatag cgtcgatatt catgaatatc
    12781 gcttcacctt ccgtgatgtc aaagaggtga ctggcccgaa ctggcgcggc ggtgtggcga
    12841 ctattggcgt aacgcgagag ggtaaaccgg aaacggtgct gtatgcggaa aaacgttatt
    12901 acaacactgc cgggtcgatg atgaccgaag cggcaattga cggcggcatc acgcgtgacc
    12961 tgtacgccgc gctcggtgaa gagctggaaa acggcgcgtg ggccgtgcgt ctttactaca
    13021 aaccatttgt tcgctggatt tgggcgggcg ggctgatgat ggcgttgggc ggactgctgt
    13081 gtctgtttga tcctcgctat cgtaagcgcg tgagtccgca aaaaactgcg ccggaggccg
    13141 tatgaagcgc aaagtattgt taattccgtt gattatcttc ctggcgattg ccgcggcgct
    13201 gctgtggcag ctggcgcgta atgccgaagg ggatgatccg accaatctgg aatcggcgct
    13261 cattggcaag cctgtgccga agtttcgtct cgaatcactg gacaatccgg ggcagtttta
    13321 tcaggcggat gtgctgactc agggcaaacc agtactgctt aacgtctggg cgacttggtg
    13381 tccgacctgc cgtgcggaac atcaatatct gaatcagctt tcagcgcagg gcattcgcgt
    13441 ggtcggcatg aactataaag acgatcgcca gaaggcaatc agctggctga aagagctggg
    13501 caatccttac gcgctaagcc tgtttgatgg cgacggcatg ttagggctgg atctcggtgt
    13561 ctatggcgcg ccagaaacct tccttattga cggcaacggt atcattcgct atcgccatgc
    13621 gggcgatctc aatcctcgcg tctgggaaga agagatcaag ccgctgtggg agaaatacag
    13681 taaggaggcc gcacaatgag gtttttattg ggcgtgctga tgctgatgat ctccggctca
    13741 gcgctggcga ccatcgacgt gttgcagttt aaagatgaag cacaggaaca acagttccgt
    13801 cagctcactg aagaactgcg ctgcccgaaa tgccagaaca acagcattgc cgattccaac
    13861 tcgatgattg ccaccgacct gcgtcagaaa gtgtatgaac tgatgcagga aggtaaaagt
    13921 aaaaaagaga ttgtcgatta tatggtggcg cgttacggca acttcgtcac ttacgatccg
    13981 ccgttaacgc cgctgaccgt gctgctgtgg gtgctgccag tagtggctat tggcattggc
    14041 ggttgggtca tatacgcccg ctcgcggcgt cgggtacgcg tggtgccgga agcgtttcct
    14101 gaacaaagcg tgccggaagg taagcgtgcc ggatatgttg tttatctgcc gggtattgtg
    14161 gtggcgttaa ttgtggctgg cgtcagctac taccagactg gcaattatca gcaggtgaaa
    14221 atctggcagc aggccacggc acaggctccg gcgttgctgg acagggcgct ggacccgaaa
    14281 gccgatccgc tcaacgaaga agagatgtcg cgccttgcgc tggggatgcg tactcaactg
    14341 caaaaaaatc cgggagatat agaaggctgg attatgttgg gccgcgttgg catggcgctg
    14401 ggtaacgcca gtatcgccac cgatgcatac gctactgcgt atcgcctcga tccgaagaac
    14461 agcgatgccg cactgggata cgctgaagcg ttgactcgtt catctgatcc caacgacaac
    14521 cgcctcggcg gtgaactgct acgtcagctg gtgagaacgg accatagcaa tatccgtgtg
    14581 ctaagcatgt atgcgtttaa tgcctttgag cagcagcgat ttggcgaagc cgttgccgcg
    14641 tgggagatga tgttgaaact cttacctgcc aacgatactc gccgtgcggt gattgaacgt
    14701 agtatcgcgc aggcgatgca acatttgtcg ccgcaggaga gtaaataaaa agaggcccga
    14761 tggtgatgcc atcgggctag actgatgaca aagtgcgctt agtttatgcc ggatgcggcg
    14821 tgaacgcctt atccggccta caaaattgtg taaattcaat aaattgatcg atccacgtag
    14881 gcctgataag cgtagcgcat caggcagttt tgcgtttgtc ataaatctca gcccgatgat
    14941 aaatccatcg ggctattttt attgtgcccc gcgttgttgc aggaacagaa tggtggccgc
    15001 cacgcgtgag cggacattga gtttacgcag cagattgcga atatgtactt ttactgtctg
    15061 ctcggaaata ttcaacaccg atgcaatctg tttatttgac agcccctgtg ccagctcgtg
    15121 cagaacatcc agctcgcgct ccgtcagcac gctgaaggga tcttcttccg cgccaaacat
    15181 ttcacgttca cgtaagtact gattgacgcg ttcgctaaag actttgctgc ctttcgctcc
    15241 ggtacgaatc gcttccagca atacttccgg gtcgctgtct ttcaacagat aaccgtctgc
    15301 gcctgcgtct atcagcgcaa agacatcgct ggaggcatcg gatacggtca ggataataat
    15361 ttgcgcggta acgccatccc tgcgcaaggc attgagggta tccaggccac tcataccttt
    15421 catattgaga tccagcaaga tcacgtcgat atccagtcta ttcgccagat cgatcgcgct
    15481 cgcgccttcg cccgcttcgg cgaccacttc aaagccagga tcaagctcca gtaactgacg
    15541 aacaccgcgt cgcataagtg gatgatcatc cacaatcatc acctgaaaag gcgttgcttc
    15601 aggcatagta gtctcctgag gttttattag aatcattatg tttgagcgta gcgttggtaa
    15661 cgctaccagg cgcgtgaatg ttaccccatt ttatgtatgg acatgtagtg aaaactacaa
    15721 ccttgtcgtt cgctttggca tcatttttat tataaagacg gaattttgca tcagcttctc
    15781 atcaagaaaa acaactaaaa aatcaatcac aatacttgtg catttcttta aacagttttt
    15841 aaaaactgac tccgggtatg gagctatggg tattttctgt acccaatgct tttaactgca
    15901 attaatttca taggatgaaa gctcaatgca tcaatctggt tctgtttctc tttgtcgttc
    15961 cgcaatatct gttctggtgg ctacagcgtt atattcaccc atagcattgg catcaacagt
    16021 tgagtatggt gagacagttg atggtgttat cctggaaaaa gatatccagc tggtttatgg
    16081 gaccgccaat aatacgaaaa tcaatcctgg cggagaacag catataaaag aatttggtgt
    16141 aagtagtaat actgaaatta acggagggta tcaatacatt gaaatgaatg gtaccgcaga
    16201 atactcagta ttaaatgatg gttatcaaat tgttcaaatg ggtggcgcgg caaaccagac
    16261 taccctcaat aatggtgtgc tacaggttta tggcccagcg aatgatacca cgattaaagg
    16321 cgggcgctta atcgttgaaa aagatggggg ggccgttttt gccgctatcg aaaagggagg
    16381 actactggag gttaaagagg ggggatttgc gcttgcggta gatcagaaaa caggcggtgc
    16441 tattaaaaca accacgcggg ccatggaggt attcggaaca aaccgtctcg gtcagttcga
    16501 tatcaagaat ggtattgcta ataatatgtt gttggaaaac ggcggaagtt tgcgagttga
    16561 agaaaatgac ttcgcttata atactactgt ggatagtggc ggcttactgg aggttatgga
    16621 tggcgggact gcaactggcg ttgataaaaa agcaggcggg aaattaattg tctcaacgaa
    16681 tgcgctggaa gtgagtggta caaacagtaa aggccaattt agtataaagg atggtgtgtc
    16741 aaaaaattat gaactggatg atggttccgg gctcattgtt atggaggaca cgcaggccat
    16801 tgacactatc cttgatgagc atgccactat gcaatcgctg ggaaaggata ctggtacgaa
    16861 agtgcaggca aatgcggtat atgatctcgg tcgatcagac cagaatggaa gtatcacgta
    16921 ttcctcaaaa gccatctctg aaaatatggt aatcaacaat ggccgcgcta atgtctgggc
    16981 tggcacaatg gttaacgtgt cagtcagagg gaatgatggc attcttgagg tcatgaagcc
    17041 gcaaataaat tatgcacccg ctatgttggt gggtaaggta gtggtttctg agggcgcttc
    17101 ttttagaacg catggtgccg tggataccag caaagcggac gtttcgctcg aaaatagcgc
    17161 atggaccatc attgccgata tcactacgac gaaccaaaac actcgcctca acttagccaa
    17221 ccttgcgatg tccggcgcaa atgtgattat gatggctgag ccagtgactc gttcatctgt
    17281 gacggcaagt gcggaaaatt tcattacgtt gaccaccaat accctgtcgg gaaacggcaa
    17341 tttttatatg cgtaccgata tggccaatca tcagagcgat cagctcaacg tcaccggtca
    17401 ggcaacaggt gatttcaaaa tattcgtgac ggacaccggt gccagcccgg cagcaggaga
    17461 tagccttaca ctggtaacaa cgggcggcgg tgatgctgca tttacgttgg gcaatgccgg
    17521 aggcgttgtt gatatcggta cgtatgaata taccttgctg gataatggca accatagctg
    17581 gagcctcgca gagaatcgcg cgcaaattac cccttcaacc actgatgtgc tgaacatggc
    17641 agccgcacaa ccgctggtat ttgatgcaga actggacacc gtgcgtgagc gtcttggtag
    17701 cgtaaaaggc gttagttacg atacggtgat gtggagttcg gcaattaaca cccgcaacaa
    17761 cgtgaccact gatgcgggag ctggttttga gcaaacattg acgggcctga cgctcggtat
    17821 cgatagccgt ttctcccgtg aagaaagcag tacaattcgc ggcttgttct ttggttactc
    17881 tcattctgat attggttttg atcgcggcgg aaaaggtaat atcgatagct ataccctggg
    17941 ggcttatgcc ggttgggagc atcagaacgg tgcctatgtt gatggggtgg tgaaagttga
    18001 ccgttttgcc aacaccatcc atggcaagat gagtaatggg gcaacagcgt ttggcgatta
    18061 caatagtaac ggcgcgggtg ctcatgttga gagcgggttc cgttgggttg acggattgtg
    18121 gagtgttaga ccctatctgg cctttaccgg ctttaccaca gatggtcagg actacacgtt
    18181 atcaaacggc atgcgcgcgg atgtgggaaa tacccggata ttacgcgctg aagcgggaac
    18241 ggcggtaagc tatcacatgg acctgcaaaa cggtacgacg ctggaaccct ggctgaaagc
    18301 cgccgtgcgt caggaatacg ccgattctaa ccaggtgaaa gttaatgacg atggcaaatt
    18361 taataatgat gtggctggaa ccagtggcgt ttatcaggct gggataaggt catcgtttac
    18421 cccgacgtta agcggtcatt tgtcagtcag ctatggcaat ggcgcagggg tagaatcgcc
    18481 gtggaatact caggcgggtg tggtctggac gttctgataa cagaaaataa acaggctgtg
    18541 atgtgtcacg gtctgtttat cgaattaatt gcagatataa aaaaaccaac cgtaagggtt
    18601 ggttttttct tgggattttt ggtcggcacg agaggatttg aacctccgac ccccgacacc
    18661 ccatgacggt gcgctaccag gctgcgctac g
//
LOCUS       DAZSNM010000067        18679 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZSNM010000067 DAZSNM010000000
VERSION     DAZSNM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18679
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.067"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..322
                     /locus_tag="ACHW42_001929"
     CDS             <1..322
                     /locus_tag="ACHW42_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8943765.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="ACHW42_001930"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="ACHW42_001930"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR8943766.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="ACHW42_001931"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="ACHW42_001931"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR8943767.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACHW42_001932"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACHW42_001932"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR8943768.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="ACHW42_001933"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="ACHW42_001933"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR8943769.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="ACHW42_001934"
     CDS             complement(3726..4955)
                     /locus_tag="ACHW42_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR8943770.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="ACHW42_001935"
     CDS             complement(5019..5459)
                     /locus_tag="ACHW42_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR8943771.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="ACHW42_001936"
     CDS             complement(5564..6334)
                     /locus_tag="ACHW42_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8943772.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="ACHW42_001937"
     CDS             complement(6331..7257)
                     /locus_tag="ACHW42_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8943773.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="ACHW42_001938"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="ACHW42_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR8943774.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACHW42_001939"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACHW42_001939"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR8943775.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="ACHW42_001940"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="ACHW42_001940"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR8943776.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACHW42_001941"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACHW42_001941"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR8943777.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="ACHW42_001942"
     CDS             12964..13311
                     /locus_tag="ACHW42_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR8943778.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="ACHW42_001943"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="ACHW42_001943"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR8943779.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="ACHW42_001944"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="ACHW42_001944"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR8943780.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACHW42_001945"
     CDS             complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACHW42_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR8943781.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACHW42_001946"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACHW42_001946"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR8943782.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVSVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
BASE COUNT         4600 a         5048 c         4560 g         4471 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgctgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggctt
//
LOCUS       DAZSNM010000068        18246 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZSNM010000068 DAZSNM010000000
VERSION     DAZSNM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18246
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.068"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACHW42_001947"
     CDS             complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACHW42_001947"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR8943783.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     regulatory      complement(1572..1768)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2005..3654
                     /gene="pgi"
                     /locus_tag="ACHW42_001948"
     CDS             2005..3654
                     /gene="pgi"
                     /locus_tag="ACHW42_001948"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR8943784.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4117..4359
                     /gene="yjbE"
                     /locus_tag="ACHW42_001949"
     CDS             4117..4359
                     /gene="yjbE"
                     /locus_tag="ACHW42_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR8943785.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            4473..5111
                     /locus_tag="ACHW42_001950"
     CDS             4473..5111
                     /locus_tag="ACHW42_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR8943786.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5108..5845
                     /locus_tag="ACHW42_001951"
     CDS             5108..5845
                     /locus_tag="ACHW42_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR8943787.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5845..7941
                     /locus_tag="ACHW42_001952"
     CDS             5845..7941
                     /locus_tag="ACHW42_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR8943788.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAISWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7988..8266)
                     /locus_tag="ACHW42_001953"
     CDS             complement(7988..8266)
                     /locus_tag="ACHW42_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8943789.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8267..8410
                     /locus_tag="ACHW42_001954"
     CDS             8267..8410
                     /locus_tag="ACHW42_001954"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8943790.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8536..8946
                     /gene="psiE"
                     /locus_tag="ACHW42_001955"
     CDS             8536..8946
                     /gene="psiE"
                     /locus_tag="ACHW42_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR8943791.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8990..10465)
                     /gene="xylE"
                     /locus_tag="ACHW42_001956"
     CDS             complement(8990..10465)
                     /gene="xylE"
                     /locus_tag="ACHW42_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR8943792.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10836..11726)
                     /gene="malG"
                     /locus_tag="ACHW42_001957"
     CDS             complement(10836..11726)
                     /gene="malG"
                     /locus_tag="ACHW42_001957"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR8943793.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(11741..13285)
                     /gene="malF"
                     /locus_tag="ACHW42_001958"
     CDS             complement(11741..13285)
                     /gene="malF"
                     /locus_tag="ACHW42_001958"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR8943794.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(13439..14629)
                     /gene="malE"
                     /locus_tag="ACHW42_001959"
     CDS             complement(13439..14629)
                     /gene="malE"
                     /locus_tag="ACHW42_001959"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR8943795.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            14994..16109
                     /gene="malK"
                     /locus_tag="ACHW42_001960"
     CDS             14994..16109
                     /gene="malK"
                     /locus_tag="ACHW42_001960"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR8943796.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            16181..17521
                     /gene="lamB"
                     /locus_tag="ACHW42_001961"
     CDS             16181..17521
                     /gene="lamB"
                     /locus_tag="ACHW42_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR8943797.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            17673..>18246
                     /gene="malM"
                     /locus_tag="ACHW42_001962"
     CDS             17673..>18246
                     /gene="malM"
                     /locus_tag="ACHW42_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR8943798.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPD"
BASE COUNT         4553 a         4726 c         4746 g         4221 t
ORIGIN      
        1 ttttctgctc attattagct ccttctgatg ccattctatt tcaggaagga gtgtccgtta
       61 aactcaggct acctcatagt gacaagaaaa tcaatacggc ccgaaatata gcttccaggc
      121 catacagtat ttactcaaac aaattactat gcagtttttg caccacctgc tcggcatctt
      181 cgccgggcac caggaagcac aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt
      241 tgaacggttc cagtacgccg aatacctctt tgccaacgcc gcaggctttt gacaggtcat
      301 tgccaatcaa cgcgaccagc gccagacctt cttccacctc cacccggcac agtgcggaaa
      361 gctccatcag cagagattgc gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat
      421 caagggttaa tgccacgctc acttctgacg tggtgattaa gtctaccgaa atattatgcc
      481 gcgcgaggat gccgaaaact tccgcgagga agccgcgaga atgcagcata ttcaggctgt
      541 gcaaagtgag cagagtctga ttgcggcgaa gcgccagcgc gcggaacagc ggcggatttt
      601 cagttttatt gcacaccagc gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga
      661 ccgggatatc gctgcgtact gcgggtagca acgttgccgg atgcagtact tttgcaccaa
      721 aagttgccat ctctgccgct tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa
      781 ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt ccagatatca acacgagatg
      841 cgtgtaaagc ctccgccagc aaggctgccg tataatcgct gcctccacgg ccaagcgtcg
      901 ttgtacgacc tttattttcg ctaccgataa atccctgggt gatcactaag ccttcattga
      961 ggcgtgggag cagctgcagc gcggccagtt ccgccagcgc ggctacatct ggctctgcac
     1021 gaccaaatcg gtcgttggta cgcatcactt tacgtacatc aaaccactgt gcctgaacat
     1081 cgcgttcgcg caggatttcg acaaacagca gggtcgacat cagctcgccg tggctgacca
     1141 gctcatctgt cagcgccgga gacgttgcca gcgccgccgc ttctgccaga acagtaatgt
     1201 tctccagcag acgttcaatc tcttcacgga taacgttcgg gtaacgcaga cgttccagaa
     1261 tggcaaactg gatgttgcga atagcgtcga gtttttcgaa tcgctcgcca ggttccagcc
     1321 cttccgctaa agcgaccagc agattagtga taccagcaga agccgagagg actactaaac
     1381 gcacgttggc atcagaaagc acaatatcag cgctgcggtt catggcgtca aaatcagcta
     1441 cgctggtacc gccaaatttg gagacaacaa tttcagacat aactacctcg tgtcagggga
     1501 tccattttca gccttggcac aagggaagag cggaagacgg gtgggcgcag agcgatactt
     1561 cgctactatt ttcacccaga agtgctccac cacttgcgaa acgcccgact gcgaacgctt
     1621 ctggtgacaa cccaggggat tcagcccctg tagccgatga tgaacgtggc cagccgttca
     1681 atcacctcgg cgatgcaccc cctcaggtgt tatcacagga ctggctcctc caacaccgtt
     1741 acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg tagtacattt ataaataaag
     1801 ggtgagcggg gcggttgtca acgacggggt catgcggatt tttcatccac tcctggcggt
     1861 cagtagttca gctaataaat gcttcactgc gctaagggtt tacactcaac attacactaa
     1921 cggcactaaa accatcacat ttttctgtga ctggcgctac aatcttccaa agtcacaatt
     1981 ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc caacgcagac cgctgcctgg
     2041 caggcactac agaaacactt cgatgaaatg aaagacgtta cgatcgccga tctttttgct
     2101 aaagacggcg atcgtttttc taagttctcc gcaaccttcg acgatcagat gctggtggat
     2161 tactccaaaa accgcatcac tgaagagacg ctggcgaaat tacaggatct ggcgaaagag
     2221 tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg agaagatcaa ccgcactgaa
     2281 aaccgcgccg tgctgcacgt agcgctgcgt aatcgtagca ataccccgat tctggttgat
     2341 ggcaaagatg ttatgccaga agtcaacgcg gtgctggaga agatgaaaac cttctcggaa
     2401 gcgattatct ccggtgagtg gaagggttat accggcaaag caatcactga cgtagtgaac
     2461 atcgggatcg gcggttctga cctcggcccg tacatggtga ccgaagcact gcgtccgtac
     2521 aaaaaccacc tgaacatgca ctttgtttct aacgtcgatg ggactcacat ctcggaagtg
     2581 ctgaaaaaag taaacccgga aaccacgctg ttcctggtag catctaaaac cttcaccact
     2641 caggaaacca tgaccaacgc ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat
     2701 gaaaaacacg ttgcgaaaca ttttgcggcg ctctcaacca atgccaaagc cgttggcgag
     2761 tttggtattg atactgccaa catgttcgag ttctgggact gggtcggcgg ccgttactct
     2821 ttgtggtcag cgattggcct gtcgattgtt ctctccatcg gctttgataa cttcgttgaa
     2881 ctgctttctg gcgcacacgc aatggacaag catttctcca ccacgcctgc cgagaaaaac
     2941 ctgcctgtcc tgctggcgct gattggcatc tggtacaaca atttctttgg tgcggaaact
     3001 gaagcgattc tgccgtatga ccagtatatg caccgtttcg cggcgtactt ccagcagggc
     3061 aatatggagt ccaacggtaa gtatgttgac cgtaacggta acgttgtgga ttaccagact
     3121 ggcccgatta tctggggtga accaggcact aacggtcagc acgcgttcta ccagctgatc
     3181 caccagggaa ccaaaatggt accgtgcgat ttcatcgccc cggctatcac ccataacccg
     3241 ctctctgatc accaccagaa actgctgtct aacttcttcg cccagaccga agcgctggcg
     3301 tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg atcagggtaa agatccggca
     3361 acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta accgcccgac caactccatc
     3421 ctgctgcgcg aaatcactcc gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa
     3481 atctttactc agggcgtgat cctgaacatc ttcaccttcg accagtgggg cgtggagctg
     3541 ggtaaacaac tggcaaaccg tattctgcca gagctgaaag atgataaaga aatcagcagc
     3601 cacgatagct cgaccaatgg tctgattaac cgctataaag cgtggcgcgg ttaatcatca
     3661 tgcgtgcctg atgccgatat gtaggccgga taaggcgttt acgccgcatc cggcaaccgg
     3721 agcctgatgc caatatcgac attaatgcat agtatgagga gttaaataac cttaaaaaca
     3781 atgccgacaa taagtcggca ttgttttatc agataaatcc ccttgtctgt gatttaacgg
     3841 aaatcatacc gtgaggttaa tcctaaaata gatttttaat cgttgtttat ttcggagaat
     3901 acgcagatta attacttttg ttttttattt taagcttatg atttttattg ttatttaaat
     3961 ataagttgaa acttatattt gatattcatt ccaattatcc taaaactcca tcgctaattc
     4021 cccgcgccgt aattcgcatg ctttagttgt gtatactcga tcccgcccga aatgtttttg
     4081 ggtaaatctc cattcattca atgaagggaa attgttatga aaaaagttct gtatggcatt
     4141 tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg cacctgtaca ggtgggcgaa
     4201 gcggcagagt cggcagcaac gtcggtttcg gcggggagtt cctccgcgac cagcgtcagc
     4261 accgtaagct cggcggtggg tgtcgcgctc gcggcaaccg gtggcggtga tggttctaat
     4321 accgggacca ccacaaccac gaccaccagt acccagtaat aaagtatgta tccccaaaat
     4381 aatttgagtc attgcatctg tggcttgaag tatgaaggga tttaccataa ccacactccg
     4441 gtgtggttat tctgcccctc tggagaagag tcgtgaagcg acctgcactc attcttatct
     4501 gcctgctatt acaggcctgt tcagccacga ctaaagagct gggcaattca ctgtgggaca
     4561 gtctgttcgg cacgccaggc gtacagctga cggacgatga tattcaaaat atgccctacg
     4621 ccagccagta catgcagctt aatggcgggc cgcaattgtt tgtggtgctg gccttcgctg
     4681 aagacggaca acaaaaatgg gtcactcagg atcaagccac tcttgtcaca caacatggtc
     4741 gtctggtgaa gactttgctt ggcggcgaca acctgattga agtgaataac cttgccgccg
     4801 acccgctgat taaacccgca caaattgttg atggtgcaac atggacgcgc acgatgggct
     4861 ggaccgagta ccagcaggta cgctacgcca ccgcacgctc agtcttcaaa tgggatggca
     4921 ccgataccgt caaggtcggc agcgatgaaa ccctggttcg cgtgctggac gaagaagtct
     4981 ccaccgacca ggcgcgctgg cataaccgct actggatcga cagcgaaggg caaattcgcc
     5041 agtcggaaca gtatctcggc gcggattatt tcccggtgaa aaccactctc atcaaggcgg
     5101 caaaacaatg attaaacaaa ctattgtcgc gttgcttttg agtgtgggag cgtcatcggt
     5161 cttcgcggca ggaaccgtca aggtgttcag caatggcagc agtgaggcca aaacgctaac
     5221 gggcgcagag catttaatcg atctggtagg gcaacctcga ctggcaaaca gctggtggcc
     5281 tggtgcggtg attagcgaag agctggcaac ggcggcagca ttgcgtcagc agcaggcgtt
     5341 gctgacacgg ctggcagaac agggcgcaga ttccagcacc gacgatgccg ccgccattaa
     5401 cgccttacgc cagcaaatcc aggcattgaa agtgacaggc aggcaaaaaa tcaatcttga
     5461 tcccgatatc gtccgcgttg ccgaacgcgg taacccgccg ttgcagggca actacacgct
     5521 gtgggtcgga ccaccgccgt ccacggtcac gttgttcggg cttatcagcc atcctggcaa
     5581 tcagccattc actccaggtc gcgacgtggc gagctatctc tctgaccaga gcctgctcag
     5641 cggtgcggat cgcagctacg cgtgggtcgt ttacccggac ggacgcacgc aaaaagcgcc
     5701 ggtggcttac tggaacaagc gtcacgtaga gccgatgccc ggcagcatta tttatgttgg
     5761 cctcgcagat tccgtctgga gtgagacgcc tgatgccctt aacgccgaca ttcttcagac
     5821 tctgacgcag cggatacccc aataatgaaa aaaagacatc tgcttagctt actggcgctg
     5881 ggcattagca cagcttgcta cggcgaaaca tatcctgcgc ccattggtcc gtcgcagtcg
     5941 gatttcggtg gcgtaggatt attacaaact cccaccgcgc gtatggcacg ggaaggggag
     6001 ttgagtctga actatcgcga taacgatcag taccgttatt actcagcttc agtgcaactc
     6061 ttcccgtggc tggaaacaac gctgcgctac accgacgtgc gcacccggca gtacagcagc
     6121 gtcgaagcgt tctctggcga tcaaacgtat aaagataaag ccttcgatct caaactgcgt
     6181 ttgtgggaag agagttactg gctgccgcaa gtggcggttg gtgcgcggga tatcggcggt
     6241 acggggctgt ttgatgcgga atatcttgtt gccagcaaag cctgggggcc gttcgatttt
     6301 acgctcggcc tgggctgggg atatctgggc accagcggta atgtaaaaaa tccgctctgt
     6361 tcagccagtg ataaatattg ctatcgcgat aacagctaca aacaggcggg atctatcgac
     6421 ggtagccaga tgttccacgg tcctgcctca ctgtttggcg gcgtggaata ccagacgccc
     6481 tggcaaccgc tgcgtctgaa actggagtat gaaggtaata attatcagca ggattttgcc
     6541 gggaagctgg agcaaaaaag taagtttaac gtcggggcga tttatcgcgt taccgattgg
     6601 gccgacgtta accttagcta tgaacgtggc aacaccttta tgtttggcgt cacgctgcgc
     6661 accaacttta acgatctgcg cccgtcctac atcgataacg cccgcccgca atatcaaccg
     6721 cagccgcagg atgccatttt gcagcattcg gtggtggcga atcagttaac gttgctgaaa
     6781 tacaacgccg gacttgccga tccgcagatc caggcgaaag gcgatacgct gtatgttacc
     6841 ggcgagcagg tgaaatatcg tgattcgcgc gaagggatca tccgtgctaa tcggatcgtg
     6901 atgaacgatc tgccggatgg gatcaaaacg atccgcatta cggaaaatcg ccttaacatg
     6961 ccgcaagtga cgacggaaac cgatgtcgcc agcctgaaaa atcatctcgc cggagagccg
     7021 ttgggccacg aaacgacgct ggcgcacaaa cgcgtcgagc cagtggttcc gaagtccacc
     7081 gagcagggct ggtatatcga caaatcacgc tttgatttcc atatcgatcc ggtgctgaac
     7141 cagtcggtcg gtggcccgga aaacttttac atgtatcagc tgggcgtgat gggaacggca
     7201 gatttgtggc tgacggacca tctactgacc accggcagcc tgtttgcaaa tcttgccaac
     7261 aactacgaca agtttaacta caccaatcca ccgcaggact cgcacttacc gcgcgttcgt
     7321 acccatgtgc gcgagtatgt gcagaacgat gtctatgtga ataacctgca agccaactac
     7381 ttccagcatc tgggcaacgg cttctacggt caggtctatg gaggttatct cgaaaccatg
     7441 tttggcggtg cgggggccga agtgttgtat cgtccgctgg acagtaactg ggcgtttggt
     7501 ctggatgcca actacgttaa acagcgcgac tggcgtagtg caaaagatat gatgaaattc
     7561 accgactaca gcgtgaaaac cggacatctg accgcctact ggacgccatc tttcgcccag
     7621 gacgtgttgg tgaaagccag cgtcgggcag tatctggcag gggataaagg cggcacgctg
     7681 gagatcgcta aacgctttga cagcggcgtg gtggtgggtg gctatgccac gatcaccaac
     7741 gtttcgaaag aggagtacgg cgaaggggac ttcaccaaag gcgtgtatgt ctcggtaccg
     7801 ttggatctct tctcgtctgg cccgacacgc agccgtgcgg cgattagctg gacgccgctg
     7861 acgcgtgacg gtggtcagca acttgggcgt aagttccagt tgtatgacat gaccagcgac
     7921 cgtagcgtaa atttccgcta agtcatggga aaggtgccag ttttcgcact cactggcacc
     7981 ctcattctta atgacctgtt gagccatcag gaacgcagtt cacccccggt ttacgatctg
     8041 gtgtgtaaag gcgaaaacat tctgtcttcc tgtccgtcga tatcggttgc aggtctggtt
     8101 gcctgagcga ctgggccatc attgggctgg agacattgct ttgcggtagg tcggctttat
     8161 cagcagtacc gagcggacca gcataagcag gaagaacaga gactgataac atcaaagcag
     8221 caaaataagg cttcattttt accaccttta tcaggttacg tttcatttgt tccagaggaa
     8281 cattgtcgat ttttcgcgca ttgctggtgg ctgggaatca cctgaatggg tgatttttga
     8341 attaccggct ttggtgcggt ttgtcttgcc ggatgcgccg ccaggcgcgg cttatccggc
     8401 ctacgggtag gtatatccgg ctttggtgga ggcgcgctcc aaatccaggt tgaacaaaac
     8461 atacacaaaa aatatagatc tccgtcacat ttttgcgtta tacaggaagc tcgccactgt
     8521 gaaggaggta ctgctatgac gtcactctct cgtccgcgcg tggagtttat ctccaccatt
     8581 ttgcagaccg tactcaatct tggcctgctg tgcctcggcc tgattttggt tgtcttcctc
     8641 ggcaaagaaa cggtgcatct ggctgatgtg ctgttcgcgc cagaacaaac cagcaaatat
     8701 gagctggtag aaggactggt ggtttacttt ctctatttcg aatttatcgc gctgattgtg
     8761 aagtactttc agtccggttt tcacttcccg ctacgttact ttgtctatat tgggatcacc
     8821 gccattgtgc gcttgatcat cgtcgatcat aaatcgccac tggatgtgtt gatctactcg
     8881 gctgcgatcc tgttactggt gatcaccctg tggctgtgta attcgaaacg gctgaagcgg
     8941 gagtaaaaag acagcacgcc gaaatggcgc ggcgtgctgg acaggaagat tacagcgtag
     9001 cagtttgttg tgttttcttc gtttccggtt cccagagcgc ttccagctcc tcaagggttt
     9061 tacctttggt ttccgggaca aatttccaca taaacagtgc tgccagaacg cccatacaac
     9121 cgtaaatcca gtaggagaaa ccgttgtgga aatgggccac cagccaggag tttttgtcca
     9181 tcatcgggaa ggtccaggag acgaagtagt tcgccagcca ctgggccgcc accgcgattg
     9241 ccagcgcttt accacgaata gcattcggga agatttccga cagcagtacc cagcataccg
     9301 gaccccagga catggcaaag gcggcaacat agaacagcat cgacagtagc gccacaatac
     9361 ccgatgcctg agtgtaaaac gcggtaccga ggctaaacat accgattgcc attccgagtg
     9421 cgccgataat ttgcagtggc ttacgaccaa atttatccac agtcataatt gccagtacgg
     9481 taaaggtgag gttgataact ccgacaataa tggtctgcaa cagcgcgata tccgtgctgg
     9541 cccccagcgt tttgaacact tccggcgcgt agtacagcac cacattgatg ccgacaaatt
     9601 gctggaagat ggagagcata acgccgatta caatcacgcc cacgccaaac atcagcagac
     9661 gaccaccggt tttgcggcca tgatccaggg agtgtttaat ttcctgtact gcctgagttg
     9721 caagcgtgtt gcccataatt ttgcgcagga tactttccgt ctgttcttgc ttgccgcgtg
     9781 acatcagcca gcgaggactt tctggcacgg tatacagcag cattaagaac agcagtgcag
     9841 ggatacattc cgaggcaaac atataacgcc tgccgtcagt attcagccag ctggcatcac
     9901 cggaacgggc aataaaatag tttacgcagt aaactaaaag ttgcccgaaa ataatcgcaa
     9961 actggttaaa tgaaaccagt tttccgcgaa tatgagctgg agccagttcc gcaatataca
    10021 ttggtgaaag cattgaggct aaaccaacgc caataccgcc aataatacga taaataacaa
    10081 attccgggac ataacctgcc agataaatag gtactgtgtt gtccgggttt atagaggtaa
    10141 aaccaagttc tggccaggca gaacctacac cagaaataaa aaacaggaca gcagaaatct
    10201 taagtgaatc acgacggccg aagcggttac tgcaataacc accgagggca ccgccgatga
    10261 tgcaaccaat cagagcgctg gctacgcaaa atcctaacag ggagttggca gcggattcac
    10321 ttaagttttg tggagcaaca aagacggtat tgagtgactc aacagtaccg gaaataacgg
    10381 cggtgtcgta gccaaataat aaaccaccta atgtagcgac taaggtaatc gaaaatatat
    10441 aactggaatt atactgggta ttcattcaga cctgccttag accattctga tcttttatca
    10501 gtgcattgat gttggaattg tcaggcagtt aaggcaatta tcatttttta caatgcgttt
    10561 acgtaatgtc ttttctgtga tcttaattgt gataattatc aaaaattgat gaaacaatac
    10621 tattgccgtg actcagagca cgaaagagaa ttatcgtaag tgggaaaaca aataacgtaa
    10681 aaataataag ttctatgatg ggaatattaa ccggcgaacg attcagattg cagacgaaag
    10741 aaaaaaaggc gctccgtgga gcgccgaata atagtcacaa gttgggataa cgtaagttga
    10801 gggtgcagcg gcataacatt ggcagaacaa catctttaac ctttcacacc acctgccgtc
    10861 aggccgttga ccagccagcg ttgagccagc aagaagacga tggtgatcgg taatgcagac
    10921 atcacggcag cggcggcaaa gtcaccccac aggtagtttt gcgggttgag gtattgctgc
    10981 atccccacgg ccagggtgta gctgtttacg tcacgcagta acagcgacgc gaccggaact
    11041 tcagtaatgg cagcgataaa cgacaggatg aataccaccg ccagaatcgg tactgacagc
    11101 ggcaacagga caaggcggaa ggcctgccac ggtgtcgcac catccagcgc agccgcttct
    11161 tccagcgaac tgtcgatggt ttcgaaatag cctttgatgg tccagacatg cagcgcaatc
    11221 ccacccagat acgcgaaaat tacgccgccg tgagtattca ggccaatgaa tggaatgtac
    11281 tcacccagac gatcaaacaa cgcatacaac gcgaccagtg aaagtactgc cgggaacatc
    11341 tggaaaatca gcattccttt cagcagcgtc gctttgcctg gaaagcgcat acgggcgaaa
    11401 gcgtaggcgc aggtggtgga gagcgccaca atgccaatcg cggaaatccc agcgaccttt
    11461 accgagttcc acagccacag cagtaccggg aatggcggtg gcgtaatgcg accatcagcc
    11521 tgttcaacgc taaaacctaa cgccagtttc cagtgatccc aggagatttg ctccgggatc
    11581 aggctaccgg tcgcgaagtt tccctgacgc agcgagatag cgacgaccat cagcagcggg
    11641 aacataatcg ccgcgataaa aagtagcagt agcaggtgag taataaacaa acgtgctttt
    11701 tgcgatttcg gttggaccat tgccattttt gttatctccc ttaatcaaac ttcattcgcg
    11761 tggctttcag gttcactatc gccagcgcac ccaccagcag gaagatcagc gtggcaattg
    11821 ctgccgccag accgaagtcc tgacccccgc cgccttcaaa agcgatacgg taggtgtagt
    11881 taacaagcag gtcggtataa ccggctggcg tggtcgtgcc aagacgatcc gggccgccgt
    11941 tggttaacag ctgaatcagc acgaagttgt taaagttaaa ggcgaagctg gcgatcatca
    12001 gcggcgtcag cggtttaatc agcagcggca gcgtaatctt aaagaagttc tggaacggac
    12061 ctgcgccatc cattgctgag gcttcataca aatcgtccgg aatcgctttc agcaagccca
    12121 tgcagaggat catcatgtac ggataaccca gccaggtatt gacgataatc agcatcgtgc
    12181 gagcggtggt cggatcgctg aaccaggcgg gcttcacgcc aaacagcgcg ctcaacatca
    12241 tgttgatttc accgaagctc tggttaaaca atcctttgaa aatcaagatt gaaatgaacg
    12301 atggcaccgc gtagggcaga atcagcagga cgcgatagac cgctttgccg cgcaacgctt
    12361 cccactgcac cagacatgcc agaaccatgc cgaccgccac cgttaaaaag acagtgatca
    12421 gcgagaacac cacggtccag acgaaaatgg cgaggaacgg tttctgaatg ccttcgtcgg
    12481 taaagacgcg ggtaaagttt ttccagccgg tggtcacggt gtaaccgggg cttagctttt
    12541 catcccccca gttgccgtcg gcggtaatgg actggtaaaa gccaatttgg ttattcggac
    12601 gatatttcac gccactctga ttattcgtca acgtgccgtc accgtcgagt gtgtagagcg
    12661 gctgcgtgcc agaaaactgg cgcagggagc tcatcatcac tttgttgcca tccggcagaa
    12721 tggcggtaat gtcactcagc gcctgacgat tctgggtaat cacgcgcaga ttcgcgcgtt
    12781 cgccttcggg ctgggcggtc gtttctttca gttgcagttt ttgctcgccg ccaaatttaa
    12841 acgcgtcgga gaggtaattt ttgccggttt cgccgtcgct gagcgccagt tgccactcat
    12901 cgcccgccgg gtaaagacca aagttatagg ttttgcctgc ttgccaggag cgatctaaca
    12961 acacttcctg cgcacgttca aaagtcagct ggttagtgct gctgtagttg gtgaaggcaa
    13021 tggcgatggt gcagaccaga gggaagagga cgaataatcc cattccagcc attcccgggt
    13081 aaacatagcg ccaggcgtag gctttacgat tggcgaaaat atacagcccc gctgaactca
    13141 atatcagcgt ggtaatggcg aacaggtatt ccccttgtgc gtacattaaa acaacaaggt
    13201 aacccaccag caggccgagc agacctagca ctgaccattt cagcgcgtcg ctttgccacc
    13261 aatgtttctt tttaatgaca tccatggggt tcttcctcat tccaggacgg ataaggcttt
    13321 cacgccttat ccgacaacaa ctgcctgatg cgacactgat gcgtcttatc aggcctacat
    13381 gcgtttcagt tttgtaggcc ggacaaggcg ttcacgccgc atccggcatt tcacagcatt
    13441 acttggtgat acgagtctgc gcgtctttca gggcttcatc gacagtctga cgaccgctgg
    13501 cggcgttgat caccgcagta cgtacggcat accagaaagc ggacatctgc gggatgttcg
    13561 gcatgatttc acctttctgg gcgttttcca tggtggcggc aatacgtgga tctttcgcca
    13621 actcttcctc gtaagacttc agcgctacgg cacccagcgg tttgtcttta ttaaccgctt
    13681 ccagaccttc atcagtcagc agatagtttt cgaggaactc tttcgccagc tctttgttcg
    13741 gactggcggc gttaataccg gtgctcagca cgccaacgaa cggtttagat ggttgaccct
    13801 tgaaggtcgg cagtaccgtt acaccataat tcactttgct ggtgtcgatg ttggaccatg
    13861 cccacgggcc gttgatggtc atcgctgttt cgcctttatt aaaggcagct tctgcgatgg
    13921 agtaatcggt gtctgcattc atgtgtttgt ttttaatcag gtcaaccagg aaggtcagac
    13981 ccgctttcgc gccagcgtta tccacgccca cgtctttaat gtcgtacttg ccgttttcat
    14041 acttgaacgc ataacccccg tcagcagcaa tcagcggcca ggtgaagtac ggttcttgca
    14101 ggttgaacat cagcgcgctc ttacctttcg ctttcagttc tttatccagc gccgggatct
    14161 cttcccaggt ttttggcggg ttcggcagca ggtctttgtt ataaatcagc gataacgctt
    14221 caacagcgat tgggtaagca atcagcttgc cgttgtaacg tacggcatcc catgtaaacg
    14281 gatacagctt gtcctggaac gctttgtccg gggtgatttc agccaacagg ccagattgag
    14341 cgtagccacc aaagcggtcg tgcgcccaga agataatgtc agggccatcg ccagttgccg
    14401 caacctgagg gaatttctct tccagtttat ccggatgctc aacggtgact ttaattccgg
    14461 tatctttctc gaatttctta ccgacttcag cgagaccgtt atagccttta tcgccgttaa
    14521 tccagattac cagtttacct tcttcgattt tggcgagagc cgaggcggaa aacatcatcg
    14581 tcgttaatgc ggataatgcg aggatgcgtg cacctgtttt tattttcata atctatggtc
    14641 cttgttggtg aagtgctcgt gaaaacacct aaacggactc tagtttcttt atacggcaac
    14701 ctctttccat cctccttgcc cctacgcccc accgttgctt tgtgtgatct ctgttacaga
    14761 attggcggta atgtggggat gcgcacataa aatcgccacg atttttgcaa gcaacatcac
    14821 gaaattcctt acatgacctc ggtttagttc acagaagccg cgttctcatc ctcccgcctc
    14881 ctcccccata aaaaagccag ggggtggagg atttaagcca tctcctgatg acgcatagtc
    14941 agcccatcat gaatgttgct gtcgatgaca ggttgttaca aagggagaag ggcatggcga
    15001 gcgtacagct gcaaaatgta acgaaagcct ggggcgaggt cgtggtatcg aaagatatca
    15061 atctcgatat ccatgaaggt gaattcgtgg tgtttgtcgg accgtctggc tgcggtaaat
    15121 cgactttact gcgcatgatt gccgggcttg agacgatcac cagcggcgac ctgttcatcg
    15181 gtgagaaacg gatgaatgac actccgccag cagaacgcgg cgttggtatg gtgtttcagt
    15241 cttacgcgct ctatccccac ctgtcagtag cagaaaacat gtcatttggc ctgaaactgg
    15301 ctggcgcaaa aaaagaggtg attaaccaac gcgtcaacca ggtggcggaa gtgctgcaac
    15361 tggcgcattt gctggatcgc aaaccgaaag cgctctccgg tggtcagcgt cagcgcgtgg
    15421 cgattggccg tacgctggtg gccgagccaa gcgtattttt gctcgatgaa ccgctctcca
    15481 acctcgatgc tgcactgcgt gtgcaaatgc gtatcgaaat ctcccgtctg cataaacgcc
    15541 tgggccgcac aatgatttac gtcacccacg atcaggtcga agcgatgacg ctggccgaca
    15601 aaatcgtggt gctggacgcc ggtcgcgtgg cacaggttgg gaaaccgctg gaactgtacc
    15661 actatccggc agaccgtttt gtcgccggat ttatcggttc gccaaagatg aacttcctgc
    15721 cggtaaaagt gaccgccacc gcaatcgatc aagtgcaggt ggagctgccg atgccaaatc
    15781 gtcaacaagt ctggctgcct gttgaaagcc gtgatgtcca ggttggagcc aatatgtcgc
    15841 tgggtattcg cccggaacat ctactgccga gtgatatcgc tgacgtcatc cttgagggtg
    15901 aagttcaggt cgtcgagcaa ctcggcaacg aaacccaaat ctatatccag atcccttcca
    15961 ttcgtcaaaa cctggtgtac cgccagaacg acgtggtgtt ggtagaagaa ggtgccacat
    16021 tcgctatcgg cctgccgcca gagcgttgcc atctgttccg tgaggatggc actgcatgtc
    16081 gtcgactgca taaggagccg ggcgtttaag caccccacaa aacacacaaa gcctgtcaca
    16141 ggtgatgtga aaaaagaaaa gcaatgactc aggagataga atgatgatta ctctgcgcaa
    16201 acttcctctg gcggttgccg tcgcagcggg cgtaatgtct gctcaggcaa tggctgttga
    16261 tttccacggc tatgcacgtt ccggtattgg ctggacaggt agcggcggtg aacaacagtg
    16321 tttccagact accggtgctc aaagtaaata ccgtcttggc aacgaatgtg aaacttatgc
    16381 tgaattaaaa ttgggtcagg aagtgtggaa agagggcgat aagagcttct atttcgacac
    16441 taacgtggcc tattccgtcg cgcaacagaa tgactgggaa gctaccgatc cggccttccg
    16501 tgaagcaaac gtgcagggaa aaaacctgat cgaatggctg ccaggttcca ccatctgggc
    16561 aggtaagcgc ttctaccaac gtcatgacgt tcatatgatc gacttctact actgggatat
    16621 ttctggtcct ggtgccggtc tggaaaacat cgatgttggc ttcggtaaac tctctctggc
    16681 agcaacccgc tcctctgaag ctggtggttc ttcctctttt gccagcaaca atatttatga
    16741 ctataccaac gaaaccgcga acgacgtttt cgatgtgcgt ttagcgcaga tggaaatcaa
    16801 cccgggcggc acattagaac tgggtgtcga ctacggtcgt gccaacctgc gtgataacta
    16861 tcgtctggtt gatggcgcat cgaaagacgg ctggttattc actgctgaac atactcagag
    16921 tgtcctgaag ggctttaaca agtttgttgt tcagtacgct actgactcga tgacctcgca
    16981 gggtaaaggt ctgtcgcagg gttctggcgt cgcgtttgat aacgaaaaat ttgcctacaa
    17041 tatcaacaac aacggtcaca tgctgcgtat cctcgaccac ggtgcgatct ccatgggcga
    17101 caactgggac atgatgtacg tgggtatgta ccaggatatc aactgggata acgacaacgg
    17161 caccaagtgg tggaccgttg gtattcgccc gatgtacaag tggacgccaa tcatgagcac
    17221 cgtgatggaa atcggctacg acaacgtcga atcccagcgc accggcgaca agaacaatca
    17281 gtacaaaatt accctcgcac aacaatggca ggctggcgac agcatctggt cacgcccggc
    17341 tattcgtgtc ttcgcaacct acgccaagtg ggatgagaaa tggggttacg actacaacgg
    17401 cgatagcaag gttaacccga actacggcaa agccgttcct gctgatttca acggcggcag
    17461 cttcggtcgt ggcgacagcg acgagtggac cttcggtgcc cagatggaaa tctggtggta
    17521 atagcaaaac ctgggccgga taaggcgttt acgccgcatc cggcataaaa acaggttgtc
    17581 attatctgaa aggggcgaaa gcccctctga ttatcgggtt tagcgcgcta ttgcctggct
    17641 accgctgagc tccagatttt gaggtgaaaa caatgaaaat gaataaaagt ctcatcgccc
    17701 tctgtttatc agcagggtta ctggcaagcg cgcctggaat tagccttgcc gatgttaact
    17761 acgtaccgca aaacaccagc gacgcgccag ccattccatc tgccgcgctg caacaactca
    17821 cctggacacc ggtcgatcaa tctaaaaccc agaccaccca actggcgacc ggcggccaac
    17881 aactgaacgt tcccggcatc agtggtccgg ttgctgcgta cagcgtcccg gcaaacattg
    17941 gcgaactgac cctgacgctg accagcgaag tgaacaaaca aaccagcgtt tttgcgccga
    18001 acgtgctgat tcttgatcag aacatgaccc catcagcctt cttccccagc agttatttca
    18061 cctaccagga actaggcgtg atgagtgcag atcggctgga aggcgttatg cgcctgacac
    18121 cggcgttggg gcagcaaaaa ctttatgttc tggtctttac cacggaaaaa gatctccagc
    18181 agacgaccca actgctcgac ccggctaaag cctatgccaa aggcgtcggt aactcgatcc
    18241 cggata
//
LOCUS       DAZSNM010000069        18158 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZSNM010000069 DAZSNM010000000
VERSION     DAZSNM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18158
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.069"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1025)
                     /locus_tag="ACHW42_001963"
     CDS             complement(<1..1025)
                     /locus_tag="ACHW42_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8943799.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1127..2107)
                     /locus_tag="ACHW42_001964"
     CDS             complement(1127..2107)
                     /locus_tag="ACHW42_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR8943800.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2115..2825)
                     /locus_tag="ACHW42_001965"
     CDS             complement(2115..2825)
                     /locus_tag="ACHW42_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR8943801.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHW42_001966"
     CDS             complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHW42_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR8943802.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5238..6647
                     /gene="glnA"
                     /locus_tag="ACHW42_001967"
     CDS             5238..6647
                     /gene="glnA"
                     /locus_tag="ACHW42_001967"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR8943803.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6820..7869
                     /gene="glnL"
                     /locus_tag="ACHW42_001968"
     CDS             6820..7869
                     /gene="glnL"
                     /locus_tag="ACHW42_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR8943804.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7881..9290
                     /gene="glnG"
                     /locus_tag="ACHW42_001969"
     CDS             7881..9290
                     /gene="glnG"
                     /locus_tag="ACHW42_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR8943805.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9402..9512
                     /locus_tag="ACHW42_001970"
     CDS             9402..9512
                     /locus_tag="ACHW42_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR8943806.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHW42_001971"
     CDS             complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHW42_001971"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR8943807.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHW42_001972"
     CDS             complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHW42_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR8943808.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(11931..12097)
                     /locus_tag="ACHW42_001973"
                     /pseudo
     CDS             complement(11931..12097)
                     /locus_tag="ACHW42_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12394..13026
                     /gene="yihA"
                     /locus_tag="ACHW42_001974"
     CDS             12394..13026
                     /gene="yihA"
                     /locus_tag="ACHW42_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR8943809.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHW42_001975"
     CDS             complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHW42_001975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR8943810.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16558..17490
                     /locus_tag="ACHW42_001976"
     CDS             16558..17490
                     /locus_tag="ACHW42_001976"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR8943811.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17531..>18158)
                     /locus_tag="ACHW42_001977"
     CDS             complement(17531..>18158)
                     /locus_tag="ACHW42_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFR8943812.1"
                     /translation="TYELDKLTKGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQK
                     QLAAHPELANDEVALQEVNAALFKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISI
                     ADPAKSSSSTNKDIKSLNFDVKLPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMM
                     TLGQMFQLITIDNNTASLQLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         4401 a         4608 c         4403 g         4746 t
ORIGIN      
        1 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
       61 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
      121 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg
      181 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
      241 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
      301 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc
      361 cagatggttt tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct
      421 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
      481 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca
      541 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
      601 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
      661 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
      721 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
      781 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
      841 agaaaaaagc ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca
      901 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
      961 ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg
     1021 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
     1081 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
     1141 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
     1201 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg
     1261 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
     1321 ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
     1381 cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa
     1441 tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga
     1501 cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga
     1561 tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg
     1621 acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt
     1681 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca
     1741 aattaccttc ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg
     1801 cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt
     1861 tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag
     1921 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcactggcga
     1981 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
     2041 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
     2101 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
     2161 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
     2221 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
     2281 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
     2341 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
     2401 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
     2461 gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
     2521 tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc
     2581 gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
     2641 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
     2701 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
     2761 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
     2821 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
     2881 gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta
     2941 atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
     3001 cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg
     3061 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
     3121 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
     3181 cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt
     3241 ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc
     3301 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
     3361 aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc
     3421 tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
     3481 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
     3541 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
     3601 cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg
     3661 ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat
     3721 accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca
     3781 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
     3841 ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct
     3901 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
     3961 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
     4021 agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata
     4081 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg
     4141 ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg
     4201 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
     4261 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg
     4321 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
     4381 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
     4441 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
     4501 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
     4561 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
     4621 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
     4681 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
     4741 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
     4801 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
     4861 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
     4921 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
     4981 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt
     5041 tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg
     5101 ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
     5161 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa
     5221 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
     5281 tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc
     5341 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
     5401 ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg
     5461 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
     5521 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
     5581 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
     5641 tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata
     5701 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
     5761 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
     5821 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
     5881 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
     5941 aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga
     6001 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
     6061 tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
     6121 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
     6181 cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt
     6241 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
     6301 gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc
     6361 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
     6421 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
     6481 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
     6541 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
     6601 gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
     6661 actacccggc gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
     6721 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
     6781 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
     6841 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg
     6901 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
     6961 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
     7021 gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt
     7081 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
     7141 atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa
     7201 gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
     7261 ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat
     7321 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg
     7381 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta
     7441 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
     7501 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
     7561 aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg
     7621 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
     7681 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
     7741 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
     7801 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
     7861 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
     7921 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
     7981 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
     8041 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
     8101 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
     8161 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc
     8221 tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
     8281 acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta
     8341 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
     8401 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg
     8461 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
     8521 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
     8581 gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
     8641 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg
     8701 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc
     8761 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
     8821 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
     8881 gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
     8941 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
     9001 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
     9061 cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc
     9121 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
     9181 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
     9241 ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
     9301 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
     9361 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
     9421 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
     9481 tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga
     9541 aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg
     9601 atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc
     9661 cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta
     9721 aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg
     9781 cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc
     9841 acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc
     9901 ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg
     9961 taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca
    10021 cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac
    10081 tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg
    10141 cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc
    10201 acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg
    10261 ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt
    10321 tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc
    10381 agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg
    10441 gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg
    10501 gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc
    10561 tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga
    10621 ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact
    10681 tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga
    10741 cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg
    10801 acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga
    10861 tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa
    10921 agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc
    10981 acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac
    11041 gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt
    11101 tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg
    11161 gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct
    11221 gtagtttaac gaataaccac accagataac atataaccgg aagggttatc agcagaacgg
    11281 gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct
    11341 tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact
    11401 catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc
    11461 ctgcttccag acgttccagc agcgcatcca gacactcatc cgtttccagt aattccaact
    11521 ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt
    11581 cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct
    11641 ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac
    11701 gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc
    11761 gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc
    11821 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    11881 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    11941 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    12001 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    12061 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    12121 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    12181 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    12241 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    12301 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    12361 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    12421 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    12481 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    12541 aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa
    12601 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    12661 gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg
    12721 caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    12781 attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa
    12841 ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt
    12901 aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg
    12961 aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc
    13021 gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa
    13081 tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt
    13141 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    13201 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    13261 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    13321 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    13381 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    13441 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    13501 cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg
    13561 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    13621 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    13681 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    13741 cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    13801 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    13861 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    13921 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    13981 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg
    14041 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    14101 atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat
    14161 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    14221 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    14281 cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    14341 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    14401 cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt
    14461 aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag
    14521 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    14581 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    14641 tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    14701 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    14761 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    14821 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    14881 aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    14941 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    15001 gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    15061 gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    15121 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    15181 tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    15241 tggcgcttcg tctgcaacac tggtttcctg aggcttcgcg gctgtttttg cccctttggc
    15301 ctgtaaccat ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    15361 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    15421 cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt
    15481 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    15541 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    15601 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    15661 gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    15721 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    15781 atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    15841 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    15901 catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    15961 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    16021 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    16081 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    16141 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    16201 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt
    16261 ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt
    16321 ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag
    16381 catgatgttt gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga
    16441 taatgggatt ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg
    16501 ttatttgggt cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg
    16561 gctaaccttt tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg
    16621 agtatcgtat tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg
    16681 attgtaaaac tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat
    16741 tttatgatgt attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg
    16801 caatgggaag ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt
    16861 aaccaccgta gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg
    16921 atgaataaat atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc
    16981 tgggcgctgg atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg
    17041 gagcgacgtg gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat
    17101 cccaccacca ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa
    17161 acccactcca cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta
    17221 aatgtactgg gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat
    17281 aaccgtcagc cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg
    17341 gatttacagc ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag
    17401 cgccattttc agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct
    17461 ctcatgtcat cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg
    17521 agtgagtatt ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct
    17581 cctgtccgtt aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt
    17641 tgtcaatcgt gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat
    17701 cagcctgctt ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg
    17761 tttctgtgac gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt
    17821 ttgtggatga tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt
    17881 cgccgagtgc gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata
    17941 acggtaaata ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg
    18001 ctaactcagg gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt
    18061 ggcgcactgc tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt
    18121 gctgattccc ttttgttaac ttatcaagtt cgtaggtc
//
LOCUS       DAZSNM010000070        18076 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZSNM010000070 DAZSNM010000000
VERSION     DAZSNM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18076
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.070"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..816)
                     /gene="rnd"
                     /locus_tag="ACHW42_001978"
     CDS             complement(<1..816)
                     /gene="rnd"
                     /locus_tag="ACHW42_001978"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR8943813.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLL"
     gene            complement(898..2583)
                     /gene="fadD"
                     /locus_tag="ACHW42_001979"
     CDS             complement(898..2583)
                     /gene="fadD"
                     /locus_tag="ACHW42_001979"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR8943814.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(2789..3370)
                     /gene="yeaY"
                     /locus_tag="ACHW42_001980"
     CDS             complement(2789..3370)
                     /gene="yeaY"
                     /locus_tag="ACHW42_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR8943815.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(3410..4105)
                     /gene="tsaB"
                     /locus_tag="ACHW42_001981"
     CDS             complement(3410..4105)
                     /gene="tsaB"
                     /locus_tag="ACHW42_001981"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR8943816.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(4163..6073)
                     /locus_tag="ACHW42_001982"
     CDS             complement(4163..6073)
                     /locus_tag="ACHW42_001982"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR8943817.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            6205..6549
                     /locus_tag="ACHW42_001983"
     CDS             6205..6549
                     /locus_tag="ACHW42_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR8943818.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            6971..7270
                     /locus_tag="ACHW42_001984"
     CDS             6971..7270
                     /locus_tag="ACHW42_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR8943819.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(7390..7569)
                     /locus_tag="ACHW42_001985"
     CDS             complement(7390..7569)
                     /locus_tag="ACHW42_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR8943820.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            7643..9004
                     /gene="pabB"
                     /locus_tag="ACHW42_001986"
     CDS             7643..9004
                     /gene="pabB"
                     /locus_tag="ACHW42_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR8943821.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            9008..9586
                     /locus_tag="ACHW42_001987"
     CDS             9008..9586
                     /locus_tag="ACHW42_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR8943822.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            9770..11134
                     /gene="sdaA"
                     /locus_tag="ACHW42_001988"
     CDS             9770..11134
                     /gene="sdaA"
                     /locus_tag="ACHW42_001988"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR8943823.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            11265..12863
                     /locus_tag="ACHW42_001989"
     CDS             11265..12863
                     /locus_tag="ACHW42_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR8943824.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(12867..14417)
                     /gene="yoaE"
                     /locus_tag="ACHW42_001990"
     CDS             complement(12867..14417)
                     /gene="yoaE"
                     /locus_tag="ACHW42_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR8943825.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(14405..14563)
                     /gene="yoaL"
                     /locus_tag="ACHW42_001991"
     CDS             complement(14405..14563)
                     /gene="yoaL"
                     /locus_tag="ACHW42_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR8943826.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            14880..15851
                     /gene="manX"
                     /locus_tag="ACHW42_001992"
     CDS             14880..15851
                     /gene="manX"
                     /locus_tag="ACHW42_001992"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR8943827.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            15914..16714
                     /gene="manY"
                     /locus_tag="ACHW42_001993"
     CDS             15914..16714
                     /gene="manY"
                     /locus_tag="ACHW42_001993"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR8943828.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            16727..17578
                     /gene="manZ"
                     /locus_tag="ACHW42_001994"
     CDS             16727..17578
                     /gene="manZ"
                     /locus_tag="ACHW42_001994"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR8943829.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            17633..>18076
                     /locus_tag="ACHW42_001995"
     CDS             17633..>18076
                     /locus_tag="ACHW42_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR8943830.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
BASE COUNT         4408 a         4728 c         4512 g         4428 t
ORIGIN      
        1 cagcagcgtt ttaccgtgaa agcggatttc gctaccggat aaacccaggc tgtccagttc
       61 gcctaaactt cccggcatat aacgcgcgac cgaccacaaa tgctcttcac gcacgacaaa
      121 gttcaccgcc agatcacgct ctcgcgcctt gcgcagtcgc cagtcggcta acagttgcag
      181 acaggccagt tggcgtgtgc gtaactgcca ggcattggtg atatcacgcc aggcatcttc
      241 cggcgcaacg acttcctgac gacgcatttg catcaggcgg cattcatcca gcgccgcagg
      301 tagccagccg gaggcctccg tttctaccat aagcttggcg gtgatcggta acagatacca
      361 gacatccgcc gctgcgtatt cacactgacg ttcggtcagc ggtctggcca gccagtcggt
      421 gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct tccaccatgg aagcgaaacc
      481 ccatgacatc gggcgtccgc agaaggcagc aaggatttgc gtgtcaatca agggttgtgg
      541 taattcgcca aagacattga ggaacacttc cagatcttca ctgcctgcat ggagaaattt
      601 tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac cagtcggtga tcccgagagg
      661 atcaattagc gccagatgct cgccatcgaa aagttgaatc aaccccagct gcgggtaata
      721 agtgcgcgta cgaacaaatt cagtatccag ggctatcgcc ggaaagacac ggacggcttc
      781 acacaaagaa gccagcgcat cgtccgtggt aatcatttgg taattcaaat tgttttctct
      841 ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct gacgactgac ttaacgctca
      901 ggctttattg tccactttgc cgcgcgcttc gtcacgtaat tctcgtcgca aaatttttcc
      961 gacgttagat ttcggtaact catcacgaaa ctccaccagc ttcggtactt tgtatcccgt
     1021 gagctgacgg cggcaaaaag tcaccagtga ctcttcggta agcgatggat cttttttcac
     1081 tacgaagatt ttcaccgctt caccactgga gccggaaggt acgccaacag ccgcgacttc
     1141 ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg ttgggataaa cgttaaaacc
     1201 ggaaaccaaa atcatgtctt ttttacgatc gacaatgcgc aggaatcctt cttcatccat
     1261 caccgcgatg tcgccggtgt gtaaccagcc atttttgatg atctcatctg tagcatccgg
     1321 acgctgccag taacccagca tcacctgcgg tcctttgaca caaagctcac ccggttgacc
     1381 cggtggtact tcgttgtcgt cgtcatccac cagtttggct tccgtcgatg gcaccggcaa
     1441 accgatgcta ccgctatgat aatcaatatc atacgggtta acgctcacca gcggcgcgca
     1501 ctcggtcagg ccataacctt ccagcagata ctgtccggtc agtttcaccc agcgttctgc
     1561 caccacttgc tgaacaggca tcccaccgcc tgcggaaaga tgcagactgg agaaatccag
     1621 ctgctggaac tctttattgt tcagcaacgc attgaacaag gtgttaacgc cagtgatagc
     1681 ggtaaacgga tatttcgcta actcttttac caaccctgga atatcacgcg ggttagtgat
     1741 aagcaggttc tgcccaccca gttcgataaa cagcaggcag ttaatggtca gggcaaaaat
     1801 gtgatacagc ggcagtgccg ttactaccag ttctttacct ggatgcagca acggcccata
     1861 ggtggcgttg acctgttcca ggttcgccag catattgcgg tgagtcagca tcgcgccttt
     1921 cgccacacct gtggtgccgc cggtgtattg caggaaggct aaatcttccg gcaccagttc
     1981 gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg ctacgaaatg aaatggcatc
     2041 tggcagatgg tatttcggca ccaagcgctt gatgtattta acaacgaaat tgactaccgt
     2101 gccttttgcc gtagatagct gatcgcccat acgggtcaga attacgtgct gaacggcggt
     2161 tttatcaacc actttttcca gagtgtgggc aaagttagac acgataacaa tcgccgatgc
     2221 gccgctatcg ttaagctgat gctcaagctc acgcggggta tacaacgggt taacgtttac
     2281 gacgattatc ccggcacgca aaatgccaaa tagcgccacc ggatattgca ataagttagg
     2341 catcatcaac gcaacgcgat cgcctttctt cagccccaac ccttgttgca aataagcggc
     2401 aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatc acctccccca tattcacaaa
     2461 cgcaggctga tcggcgtagc gcgcgaccga ctgctcaaac atatctacca gagattgata
     2521 acggtcaggg ttgatctccg tcggaacgtc cgcgggataa cggttaagcc aaaccttctt
     2581 caattcttca cctctaaaat gcgtgttcgt cgtcatcgca accccaaatg atatacacgc
     2641 cgttaacata atattaactc atcataccag cttgataatt acccaacgaa aaggttgcga
     2701 agcgcgtcac tatttatttt tatctttacc gtaagaatgc agaaacagcg gaccagccgc
     2761 tgtttctgca ttctttaaaa acaagcaatt attcggttac aactgtttgt actctcgcgg
     2821 ggccgggatt ataccagccc catccgccgt agccataggg ccagccacga ccgccataaa
     2881 accatggatc aatcggctga ggcggcataa tcacctgctg ggttaaatgc caacgtttgt
     2941 aacccgttac ttgcatcacc ataaatttat agggcgtatt gccgattttg ccgtcaaccg
     3001 caccggtgat tggcccgact accgtaacca gttgtccacg gaaatccacc gggtccagaa
     3061 aaccgttcac atcggcataa atgcgaccgc gagaaggttc tcccagcgtc ggcctagctc
     3121 cgctgtccag cggtacggta gcaatttcca ggcgggtttt cccttgctgg ttttgtaccg
     3181 caaccacttt gccaccaaag cgtgcctcct gaccaacgta cagctgcggc gcactcatca
     3241 cccgaactaa atcttgttgc ggcgtggtac tgctgccttt aatggcgtcc ggcacagtga
     3301 cacaaccgct cagcattagc gcaaacgtac ctgccagtat gcctttgata acattctttt
     3361 gaaccgccat gatgcgactc ctttttttct cagggcatac tcttaagatt cattctttgc
     3421 ccggaagttt cttccatgcg acgttgttac gcaaataaac cggctcggca tgttccaccg
     3481 ccaccgtttt accctcggca aacatctgac aagcaatcgg cagcatatct tctgcagcgg
     3541 ggagtaacac ttcgccatcg cgcaaaacca gcccgctctc tttaccgaga tccggccagg
     3601 cttgccagcc cgtccctacc gtcacccatt cgccggaaag ctgttgcatt cgttcatgaa
     3661 cgagttcggg tttgagtacg gcttcggttt cttcaccgtg ccagataccg ttttcatcac
     3721 gctgatattc ggcccagtaa acttcgccca ttcgcgcatc aatggctgac agcacgcgtg
     3781 ttgcgccgtt tttgcgccac gccccttgcg ccatcgtcat tagcgtggag acgccaatca
     3841 tcggtaattc cgcgccaagc gccagccctt gcgcgatgcc aataccaatg cgcacgccgg
     3901 taaagctacc ggggccgcgc ccgtaagcca gagcgttaat atcagtcagg gaagttccgc
     3961 tggtggtcag gatatcctgc accatcggta agattcgttg agtatgttca cgagggcaaa
     4021 gctcaaaatg agcgttgaca gtaccgtcgt tccacagggc gacagagcag gcctctgtcg
     4081 cggtatcgat agccagaatt cgcatgggtc ttcgtgctta gatcaataaa aaggcgcgca
     4141 tcataccata ctccgtaaca aattacctgg aggatggtat cgcaaggaaa cgaaccgcac
     4201 gggcaatgtc acgggtgcgt ggcgcgggcg gcagactggc gagaaacgtc gcgccgtaag
     4261 gacgcatcac cagccgatag tcacaaatca ccagcacgcc acgatcgtcg gcgtcgcgaa
     4321 tcagtcgacc taccccctgt ttgagagtaa tgacggcatc tggtagttgc acttcatcga
     4381 acgggtcgcc accgcgcaaa cgacaatctt ccatgcgcgc ttttaacagt ggatcatccg
     4441 gcgaggtaaa cggcaatttg tcgataatta ccaatgacaa tgtatcgcca cgcacgtcca
     4501 ccccttccca gaaactactg gttgccacaa gaagcgcatt accggcactg acaaactgct
     4561 gcaacagttg gcctttactg gtttccccct gcaacaaaac gggaagcgtc atggtagcgc
     4621 ggaactgctc ggccagatcg cgcatcatgg cgtgcgaggt acaaagcata aaacaacgac
     4681 cgttgttagc ttcgatgatc ggtcgcagca ttgccgctaa ctggcgagca gaacctggct
     4741 ggttggtttg cggcagattg cgcggcacac agagtaacgc ctggcggctg taatcaaacg
     4801 ggcttggcaa tagcaacgac tccgcctgtt ctatcccaag ccgcgaggtg aaatgatgca
     4861 gatcgtcgtt caccgacagc gttgctgagg tgaagatcca gctaccgggc ttttgcgcca
     4921 ttaactcttt gaatttatcc gccacgctga gcggcgtgag agccagagta aaatggcgcg
     4981 aagtgcattc gtaccagtag ctgtagcccg gctgattgat ctcttttagc cgcttcagcc
     5041 gtgtgcgata caacgtggcg cgctcaaatg ccgcatccag caaggcggaa cgccccagtg
     5101 acagtttcgc cacatcataa caaagttcca aggtgtcatc gagcagtaaa aatgcccgct
     5161 gaatttgcgg attagctaac agctcacgca ggttaccgcg ataacctggc tcaccgagtt
     5221 gcagacgaaa atcctgcgca ctctgggcaa gacgatcagc gcacttttgt aactgctggg
     5281 tgtcttttaa ttcggtgcgg taggcgatgg tgatgtcttt tgccaggtcg agcagttgtc
     5341 gactggagag tgactgacca aaatactggc tggcaatgtc cggtagctgg tgggcttcgt
     5401 cgaagatcat gacgtccgct tcagggatca actcgccaaa tccactctct ttaaccacca
     5461 tatccgccag aaagagatga tggtttacca ccaccacatc ggcgtccatc gcttttttac
     5521 gtgctttgac cacaaagcaa tctttataca tcgggcagtc gctgccaaga cagttgtcgt
     5581 tggtgctggt gaccagcggc cacgcctgtg aatcttccgc cacgctgacg caggtgctga
     5641 tatcaccatc gactgtttga ttagaccagg agcgcagcag gatcacatcg cttaagattt
     5701 gtaccggcag atcgcccccc gccagcgcct gctgttcgag acgttcgagg cagaggtagt
     5761 ttgagcgccc tttcagcagc gccacgttgc ccgtatattt caatgccttt gagactgttg
     5821 gcaaatcgcg gctgtagagc tgatcctgca acgcttttga gccggtcgag ataatgactt
     5881 tctttttcgc ccgcagcgca ggagccaggt aagcgtaggt tttgcccgta ccggttcctg
     5941 cttccaccac cagcggctgg cctttttcta tcgcctgggt gacggctacc gccatctgtc
     6001 gctgtggttc tcgcggctta aagcctggta tcgctttcgc cagctgaccg tctggtgcaa
     6061 aatcgtccgt cacactaccc cctgttgatt tgaacaggga ttatgtcagg atgagggcgc
     6121 tttcgccagt tgaagtggtg acggcaacct cacattgtgg cagtctttgc agcacgaaaa
     6181 tggaagtata atgaggacaa aatgatgact atcgttcgta tcgatgctga agcccgctgg
     6241 tctgatgtag taatccacaa caacacgctc tactacactg gtgtaccgga aaacctcgac
     6301 gccgatgcct ttgagcaaac cgccaacacg ctggcacaga ttgacgccgt gctggaaaaa
     6361 cagggcagca ataaatcgag cattctggat gccaccattt tcctggccga taaaaacgac
     6421 ttcgcggcga tgaataaagc gtgggatgcc tgggttgtcg cgggtcatgc gccggtgcgc
     6481 tgcacggtac aagcgggttt gatgaacccg aagtataaag ttgaaattaa gattgtggct
     6541 gcggtgtaag ctttatcgaa gcaaaataag tcagacgata atttatcgat aatactggtc
     6601 ggttttacat aaatcgacca gagaataaga tattactgtt gatgcttctg ctaattcatt
     6661 atttatattt ataatttcaa ttttatctat aaaactaact atataaataa acataaccat
     6721 gccgttgtgt tcgccctttg attagcaact ctggttttct ttcttagctg aacaatctgg
     6781 ttatagcgtg cgggctgacc gttaattttc tccatcttac tggataacac accatgagct
     6841 gctaccttta aaacacctga atgtcaacag gttaactcgt gccatatcgt tataatccct
     6901 ttgatattag atgcaaatta aggtcatata tagccttatc gataacatgg ttaattttaa
     6961 ggataagaat atgcctgctg taatagataa agccctggat ttcattggtg ccatggatgt
     7021 atcagcgcca acaccaagtt cgatgaatga aagcacggcg aagggaatct ttaaatattt
     7081 aaaagaactg ggagtacccg cgagtgccgc tgatattacg gcgcgagctg accaggaagg
     7141 ctggaaccct gggttcacgg aaaaaatggt tggatgggca aaaaaaatgg agacaggtga
     7201 acgctctgtg attaaaaatc ctgaatactt ttcaacatat atgcaggaag aactaaaagc
     7261 actggtctga gttaaattta tatcagcata aatgggtcag gacgctttta atcacatata
     7321 aaaagcgtcc tttttctcac aatcaaatga tcatcggctt ctataacgaa atcgatacgg
     7381 attagtgtgt tactcgtctt catcctcaaa acgcgccacg attagctcgc cggagtggtt
     7441 ggcacgcagc tcttctgcca ccagcgcgat tgcctggccg ctactcatcc cttgtgccat
     7501 cagttcctgg atccgctcga cagctttttg ctgctgttca tggatgagtg aaggtaaacc
     7561 tgcaaacatt gttaactcct gctaaattgt tggcgctaat tatttcatgc tacccggcac
     7621 atagccagta gagtcaggac tgatgaagac gttatctccc actgtgatta ctttaccctg
     7681 gcgtcaggac gccgctgaat tttatttctc ccgcttaagc cacctgccgt gggcgatgct
     7741 tttacactcc ggctatgccg atcatccgta tagccgcttt gatattgtgg tcgccgatcc
     7801 gatttgcact ttaaccactt taggtaaaga aaccgttgtt agtgaaagcg aaaaacgcac
     7861 aacgaccact gatgacccgc tacaggtgct ccagcaggtg ctggatcgcg cagacattcg
     7921 cccaacgcat aacgaagatt tgccatttca gggcggcgca ctggggttgt ttggctacga
     7981 tctgggccgc cgttttgagt cactgccaga aattgcggaa caagatatcg ttctgccgga
     8041 tatggcagtg ggtatctacg attgggcgct cattgtcgac caccagcgtc atacagtttc
     8101 tttgctgagt cataatgatg tcaatgcccg tcgggcctgg ctggaaagcc agcaattctc
     8161 gccgcaggaa gatttcacgc tcacttccga ctggcaatcc aatatgaccc acgagcagta
     8221 cggcgaaaaa tttcgccagg tacaggaata tctgcacagc ggtgattgct atcaggtgaa
     8281 tctcgcccag cgttttcatg cgacctattc tggcgatgaa tggcaggcat tccttcagct
     8341 taatcaggcc aaccgcgcgc catttagcgc ttttttacgt cttgaacagg gtgcaatttt
     8401 aagcctttcg ccagagcggt ttattctttg tgataatagt gaaatccaga ctcgcccgat
     8461 taaaggcacg ctaccacgcc tgcccgatcc tcaggaagat agcaaacaag cagaaaaact
     8521 ggcgaactca gcgaaagatc gtgccgaaaa tctgatgatt gtcgatttaa tgcgtaatga
     8581 tatcggtcgt gttgccgtag ccggttcggt aaaagtacca gagctcttcg tggtggaacc
     8641 cttccctgcc gtgcatcatc tggtcagcac tataacggcg cgactaccag aacagttaca
     8701 cgccagcgat ctgctgcgcg cagcttttcc tggtggctca ataaccgggg ctccgaaagt
     8761 acgggctatg gaaattatcg acgaactgga accgcagcga cgtaatgcct ggtgcggcag
     8821 cattggctat ttgagctttt gcggcaacat ggataccagc attactatcc gcacgctgac
     8881 tgccattaac ggacaaatat actgctctgc gggcggtgga attgtcgccg atagccagga
     8941 agaagcggaa tatcaggaaa cttttgataa agttaataag atattacgcc aactggagaa
     9001 gtaagacgtg gaataccgta gcctgacgct tgatgatttt ttatctcgct ttcaactttt
     9061 gcgcccgcaa attaaccggg aacccctaaa tcatcgtcag gctgccgtgt taatccccat
     9121 cgtccgtcga ccgcaaccgg ggttgttgct gactcagcgt tcaattcatc tgcgtaaaca
     9181 cgctggacaa gtggcattcc ctggaggtgc agtcgatgac acggacgcat cagttatcgc
     9241 cgccgcgctg cgcgaagctg aagaagaggt cgctataccg ccttccgccg ttgaagttat
     9301 cggcgtgctg ccgcccgtcg atagcgtaac tggctatcaa gtaaccccag tggtcggcat
     9361 tatcccgccc gatctgccgt atcgcgccag tgaagatgaa gtctcggcgg tgtttgaaat
     9421 gccgctcgcc caggcattac atctgggtcg ttatcaccct ttagatatct accgccgtgg
     9481 cgattcacat cgggtatggc tgtcctggta cgaacagtat tttgtatggg gaatgaccgc
     9541 aggcataatt cgtgagctgg cgctgcaaat tggtgtgacc ccctgactat acttatcttt
     9601 acatctacaa aacactactt gagacaatca tcgcaatatt agttaaatcg cggtttttga
     9661 ttagtttaat tcatgtgaat agttaagcca gtcgccgcgt tccctcttac actatgcgct
     9721 gttattagtt cgttactgga agtccagtca ccttgtcagg agtattatcg tgattagtct
     9781 attcgacatg tttaaggtgg ggattggtcc ctcatcttcc cataccgtag ggcctatgaa
     9841 ggcgggtaaa cagttcgtcg atgatctggt cgaaaaaggc ttactggata gcgttactcg
     9901 cgttgccgtg gacgtttatg gttcactgtc gctgacgggt aaaggccacc acaccgatat
     9961 cgccattatt atgggtcttg caggtaacga acctgccacc gtggatatcg acagtattcc
    10021 cggttttatt cgcgacgtaa aagagcgcga acgtctgctg ctggcacagg gacggcatga
    10081 agtggatttc ccgcgcgaca acgggatgcg ttttcataac ggcaacctgc cgctgcatga
    10141 aaacggtatg caaatccacg cctataacgg cgatgaagtc gtctacagca aaacttatta
    10201 ttccatcggc ggcggtttta tcgtcgatga agaacacttt ggtcaggatg ctgccaacga
    10261 agtgagcgtg ccgtatccgt tcaaatctgc caccgaactg ctcgcgtact gtaatgaaac
    10321 cggctattcg ctgtctggtc tcgctatgca gaacgaactg gcgctgcaca gcaagaaaga
    10381 gatcgacgag tatttcgcgc atgtctggca aaccatgcag gcatgtatcg atcgcgggat
    10441 gaacaccgaa ggtgtactgc caggcccgct gcgcgtgcca cgtcgtgcgt ctgccctgcg
    10501 ccggatgtta gtttccagcg ataaactgtc taacgatccg atgaatgtca ttgactgggt
    10561 aaacatgttt gcgctggcag ttaacgaaga aaacgccgcc ggtggtcgtg tggtaactgc
    10621 gccaaccaac ggtgcctgcg gtatcgttcc ggcagtgctg gcttactatg accactttat
    10681 tgaatcggtc agcccagaca tctatacccg ttactttatg gcagcgggcg cgattggtgc
    10741 attgtataaa atgaacgcct ctatttccgg tgcggaagtt ggttgccagg gcgaagtggg
    10801 tgttgcctgt tcaatggctg ctgcgggtct tgcagaactg ctgggcggta gcccggaaca
    10861 ggtttgcgtg gcggcggaaa ttggcatgga acacaacctt ggtttaacct gcgacccggt
    10921 tgcagggcag gttcaggtgc cgtgcattga gcgtaatgcc attgcctctg tgaaggcgat
    10981 taacgccgca cggatggctc tgcgccgcac cagtgcaccg cgcgtctcgc tggataaggt
    11041 catcgaaacg atgtacgaaa ccggtaagga catgaacgcc aaataccgcg aaacctcacg
    11101 cggtggtctg gcaatcaaag tccagtgtga ctaatacttc ttactcgccc atctgcaacg
    11161 gatgggcgaa tttatacccg ctttctcgtc tgctgtaata ttccccacta cacttccact
    11221 gttgcgtcag gcgtttgtcg ccatacgctt acagggtggc ccgcatgcaa aaagcacaac
    11281 ggatcattaa aacctatcgc cgtaatcgaa tgattgtttg tacgatttgc gcactcgtta
    11341 cgctcgcttc gaccctgagc gtgcgattta tttcacagcg taacttaaat caacaacggg
    11401 tagtacaatt cgccaatcac gctgtagagg aattagataa agtactgctt cccctacagg
    11461 caggtagcaa agtcttgctt ccgctgattg gtctgccctg ctctgtcgcc catttgccat
    11521 tacgtaaaca ggcggcaaaa ctccaaactg tgcgatccat tggcctggtg caagacggca
    11581 cactttattg ctccagcatt tttggttatc gcaatgtgcc cgtcgtggac attctggctg
    11641 aacttcctgc accgcaacca cttttacgcc tgacgatcga ccgtgccctg attaaaggca
    11701 gtccggtttt gattcaatgg acgcctgcag cgggcagtag caatgctggg gtcatagaga
    11761 tgattaacat cgacttactg acggcaatgc tccttgagcc acaactgccg caaatcagta
    11821 gcgccagcct gacggtggac aaacggcatt tgctctatgg taatgggctg gtagattccc
    11881 ttccgcaacc tgaagacaat gaaaactatc aggtttcttc gcaaagcttt ccttttacca
    11941 ttaacgtaaa tggtccgggg gctacggcgc tggcatggca ctatcttcca acacaattac
    12001 cgctggcggt gctgctaagt ttactggtgg gctatatcgc ctggctggcg accgcttacc
    12061 ggatgagctt ttcccgcgaa attaatctgg gcctggcgca acatgagttc gaattgttct
    12121 gtcagccttt gcttaatgcg cgcagccagc aatgtattgg tgtagagatt ttgctgcgct
    12181 ggaataatcc gcgtcagggc tggatttcac cggatgtgtt tattcctatc gcggaagaac
    12241 atcatttaat tgtgccactg acccactatg tgatggcaga aaccattcgt cagcaccatg
    12301 ttttcccgat gagtagtcag tttcatgttg gcattaacgt cgcacccagc cattttcgcc
    12361 gtggtgtgct gataaaagat ctcaatcagt actggtttag cgctcacccg attcagcaac
    12421 tgatcctcga aatcaccgaa cgcgatgcct tactggatgt tgattatcgg attgcccgcg
    12481 agctgcatcg taaaaacgtc aaactggcga ttgatgactt cggcaccggc aacagctcgt
    12541 tttcctggct tgaaacatta cgtcctgacg tgctgaaaat tgataagtca tttaccgcag
    12601 ctataggttc tgacgcggtt aactcgacgg tgaccgatat catcatcgcg ctggggcaaa
    12661 gactgaatat tgaactggtg gcggagggcg tggaaacgca agaacaggcg aagtatttgc
    12721 gccgtcatgg cgtgcatatt ttgcaagggt atttgtacgc acagccgatg ccgctacgtg
    12781 attttcccaa atggctggcg ggcagccaac cgccgcccgc ccggcataat ggacatatca
    12841 cgcccattat gtcgttacgt taacgcttac tcatcttcat cgtgcgccgg ttgctcttta
    12901 acaatgcgaa ccagatcaac acgataatca ttggcttcaa agatggtgat atgcagtggc
    12961 cctacatcaa tcacatcgcc cacacgggga atgtgaccat ttgccgagat cacgaggccc
    13021 gcgaccgtcg cgatatcatc gtcatcggca aggtgctcaa catcaagcgc ctgctgcaag
    13081 gcatgcaaat ctgtaccgcc ttttaccagc cagccgttac catcggtaat gatttccggc
    13141 gtttcgtcag cgtccgggaa ttcacccgca atggcttcca gcacatccag cggcgtgacc
    13201 aagccttgta ccacaccaaa ctcgttggtc acgataacaa agctcccgcg agcgcgacgc
    13261 agcacgccca gcaggttgat cggatcgagg gtttccggga cgataatcgc cggagacgcc
    13321 gaagcaatcg ccgccacatc aacgccctct tccagcgcca ccagcagttc tttagcacgt
    13381 acgataccga tgatttcatc cagttcaccg cgacataccg ggaacagact gtgcggtgaa
    13441 gagagcagtt gctcgcggat ttcatcgacc ccgagattag cgtcaaccca gcttatttca
    13501 ccgcgcggcg tcatgatccc gcgcagagaa cgcgacgcca gcgtcagtac gccgttaatc
    13561 atgtaccgtt cttcttcggc aaatgcacct tccgggatcg gcatcgggtt atcggcatcg
    13621 tgctgaacat tggcctgacg tttcccgccc atcaaacgca ggatggcatc ggcagtacgc
    13681 gctcgcagcg gcaaagtcga ctggtgacga ataaagttgc gacgcgcaat ctggttaaac
    13741 acttcgataa tgatcgagaa gccaatcgcg gcatacaggt aacctttcgg aatgtggaaa
    13801 ccgaaacctt ctgccaccag actcagacca atcattaaca ggaagctcag acagagcacc
    13861 accaccgtgg ggtgctggtt aacgaatcgc gtcagcggtt tcgatgccag caacataacc
    13921 gccatcgcaa tcactaccgc cgccatcatc accggcagat ggttaaccat ccctactgca
    13981 gtaattaccg catccaacga gaagacggcg tcaaggatga caatctgtgt gacgaccacc
    14041 cagaaactgg cgtagccttt accgtggccg gaatcatgat cgcggttttc cagccgttca
    14101 tgcagttcgg ttgttgcttt gaacagcaag aatatccccc cgaacaacat aatcagatcg
    14161 cgtccggaga aggagaaatc catgacggta aatagcggtt tggtcagcgt gaccatccat
    14221 gaaatcagcg acagcagccc cagacgcata atcagcgcca gtgataaccc cagcaaacgc
    14281 gctttatcgc gttgttttgg cggcagtttg tcagcaagaa tggcgatgaa gaccaggtta
    14341 tcgataccca gcacaatttc gagaacaaca agcgtgagta gccccgccca aattgagggg
    14401 tccattaaga attccatgac aagctcctgc ttaaggaata gctattcgac gccagaaata
    14461 atgcaggcgt aacgacaaaa tgcaaacgaa aggtgcggca tagagtgcca gaaaggcagg
    14521 cgttaaaagg cctgatgctg aaatgacgtc ggtgacgatc catactgcgg gctactgccc
    14581 tatactccat ggttgttaaa cgggagttaa acatatcaga gacgcctctg atttggcaaa
    14641 gatttacctt cctttgcaaa cgaatgtgac aaggatattt tacctttcga aatttctgat
    14701 aatcgaaagt taaattacgg atcttcatca cataaaataa ttttttcgat atctaaaata
    14761 aatcgcgaaa cgcaggggtt tttggttgta gcccttatct gaatcgattc gattgtggac
    14821 gacgattcaa aaatacatct ggcacgttga ggtgttaacg ataataaagg aggtagcaag
    14881 tgaccattgc tattgttata ggcacacatg gttgggctgc agagcagttg cttaaaacgg
    14941 cagaaatgct gttaggcgag caggaaaacg tcggctggat cgatttcgtt ccaggtgaaa
    15001 atgccgaaac gctgattgaa aaatacaacg ctcagttggc aaaactcgac accactaaag
    15061 gcgtgctgtt tctcgttgat acatggggag gcagcccgtt caatgctgcc agccgcattg
    15121 tcgtcgacaa agagcattat gaagtcattg caggcgttaa cattccaatg ctcgtggaaa
    15181 cgttaatggc ccgtgatgat gacccgagct ttgatgaact ggttgcgctg gcagtagaaa
    15241 caggccgtga aggcgtgaaa gcactgaaag ccaaaccggt tgaaaaagcc gcgccagcac
    15301 ccgctgccgc agcaccaaaa gcggctccaa ctccggcaaa accaatgggg ccaaacgact
    15361 acatggttat tggccttgcg cgtatcgacg accgtctgat tcacggtcag gtcgccaccc
    15421 gctggaccaa agaaaccaat gtctcccgta ttattgttgt tagtgatgaa gtggctgcgg
    15481 ataccgttcg taagacactg ctcacccagg ttgcacctcc gggcgtaaca gcacacgtag
    15541 ttgatgttgc caaaatgatt cgcgtctaca acaacccgaa atatgctggc gaacgcgtaa
    15601 tgctgttatt taccaaccca acagatgtag agcgtctcgt tgaaggcggc gtgaaaatca
    15661 cctctgttaa cgtcggtggt atggcattcc gtcagggtaa aacccaggtg aataacgcgg
    15721 tttcggttga tgaaaaagat atcgaggcgt tcaaaaaact gaatgcacgc ggtattgagc
    15781 tggaagtccg taaggtttcc accgatccga aactgaaaat gatggatctg atcagcaaaa
    15841 tcgataagta acgtattgtg ttgattatca ttcagttttc acacttaagt cttacgtaaa
    15901 caggagaagt acaatggaga ttaccactct tcaaattgtg ctggtattta tcgtagcctg
    15961 tatcgcaggt atgggatcaa tcctcgatga atttcagttt caccgtccgc taatcgcgtg
    16021 taccctggtg ggtatcgttc ttggggatat gaaaaccggt attattatcg gtggtacgct
    16081 ggaaatgatc gcgctgggct ggatgaacat cggtgctgca gttgcgcctg acgccgctct
    16141 ggcttctatc atttctacca ttctggttat cgcaggtcat cagagcattg gtgcaggtat
    16201 cgcactggca atccctctgg ccgctgcggg ccaggtactg accatcatcg ttcgtactat
    16261 taccgttgct ttccagcacg ctgcggataa ggctgctgat aacggcaacc tgacagcgat
    16321 ttcctggatc cacgtttctt ctctgttcct gcaagcaatg cgtgtggcta ttccggccgt
    16381 catcgttgcg ctgtctgttg gtaccagcga agtacagaac atgctgaatg cgattccgga
    16441 agtggtgacc aatggtctga atatcgccgg gggcatgatc gtggtggttg gttatgcgat
    16501 ggttatcaac atgatgcgtg ctggctacct gatgccgttc ttctacctcg gcttcgtaac
    16561 cgcagcattc accaacttta acctggttgc tctgggtgtg attggtactg ttatggcagt
    16621 gctctacatc caacttagcc cgaaatacaa ccgcgtagcc ggtgcgcctg ctcaggcagc
    16681 tggtaacaac gatctcgata acgaactgga ctaacaggtg agcgaaatgg ttgatacaac
    16741 tcaaactacc accgagaaaa aactcactca aagtgatatt cgtggcgtat tcctgcgttc
    16801 taacctcttc cagggttcat ggaacttcga acgtatgcag gcactgggtt tctgcttctc
    16861 tatggtaccg gcaattcgtc gcctctaccc tgagaacaac gaagctcgta aacaagctat
    16921 tcgccgtcac ctggagttct ttaacaccca gccgttcgtg gctgcaccga ttctcggcgt
    16981 aaccctggcg ctggaagaac agcgtgctaa tggcgcagag atcgatgacg gtgctatcaa
    17041 cggtatcaaa gtcggtttga tggggccgct ggctggtgta ggcgacccga tcttctgggg
    17101 aaccgtacgt ccggtatttg cagcactggg tgccggtatc gcgatgagcg gcagcctgtt
    17161 aggtccgctg ctgttcttca tcctgtttaa cctggtgcgt ctggcaaccc gttactacgg
    17221 cgtagcgtat ggttactcca aaggtatcga tatcgttaaa gatatgggtg gtggcttcct
    17281 gcaaaaactg acggaagggg cgtctatcct cggcctgttt gtcatggggg cattggttaa
    17341 caagtggaca catgtcaaca tcccgctggt ggtttcgacc atcaccgggc aggatggaca
    17401 gacccgtgtc accaccgtgc aaactattct tgaccagtta atgccaggcc ttgtaccact
    17461 gctgctgaca tttgcttgta tgtggctgct gcgcaaaaaa gttaacccgc tgtggatcat
    17521 cgttggcttc ttcgtcatcg gtatcgctgg ttacgcttgc ggcctgctgg gactgtaaga
    17581 ctgtcgtaca ctaccggggc cttttggccc cgttttttta tctggaggat taatgacaat
    17641 cacggacctg gtactgattc ttttcatcgc cgcactcctg gccttcgcga tctacgatca
    17701 gttcatcatg ccccgccgta acggccccac cctgctggca attcctttgc tccggcgtgg
    17761 tcgcatcgat agcgttatct tcgtcggatt gattgtcatt cttatctata acaacgtcac
    17821 gaatcatggc gcgctaataa caacatggtt attaagtgca ctggctctga tgggttttta
    17881 tatattctgg atccgcattc cgaagatcat ctttaaacaa aaaggttttt tcttcgccaa
    17941 tgtctggatt gaatatagcc gaatcaaagc gatgaacttg tcggaagatg gcgtgttggt
    18001 gatgcaatta gaacagcgtc ggctgttaat ccgcgttcga aatatcgacg atctggaaaa
    18061 aatttataag cttctc
//
LOCUS       DAZSNM010000071        17942 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZSNM010000071 DAZSNM010000000
VERSION     DAZSNM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17942
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.071"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            130..654
                     /locus_tag="ACHW42_001996"
     CDS             130..654
                     /locus_tag="ACHW42_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFR8943831.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            735..1409
                     /locus_tag="ACHW42_001997"
     CDS             735..1409
                     /locus_tag="ACHW42_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR8943832.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1425..3887
                     /locus_tag="ACHW42_001998"
     CDS             1425..3887
                     /locus_tag="ACHW42_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR8943833.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            3903..4936
                     /locus_tag="ACHW42_001999"
                     /pseudo
     CDS             3903..4936
                     /locus_tag="ACHW42_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5018..5356)
                     /gene="rcnB"
                     /locus_tag="ACHW42_002000"
     CDS             complement(5018..5356)
                     /gene="rcnB"
                     /locus_tag="ACHW42_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR8943834.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5575..6405)
                     /gene="rcnA"
                     /locus_tag="ACHW42_002001"
                     /pseudo
     CDS             complement(5575..6405)
                     /gene="rcnA"
                     /locus_tag="ACHW42_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6527..6799
                     /gene="rcnR"
                     /locus_tag="ACHW42_002002"
     CDS             6527..6799
                     /gene="rcnR"
                     /locus_tag="ACHW42_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR8943835.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6891..6987
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7022..7810
                     /gene="thiM"
                     /locus_tag="ACHW42_002003"
     CDS             7022..7810
                     /gene="thiM"
                     /locus_tag="ACHW42_002003"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR8943836.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7807..8607
                     /gene="thiD"
                     /locus_tag="ACHW42_002004"
     CDS             7807..8607
                     /gene="thiD"
                     /locus_tag="ACHW42_002004"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR8943837.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8672..9489
                     /locus_tag="ACHW42_002005"
                     /pseudo
     CDS             8672..9489
                     /locus_tag="ACHW42_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
     gene            9541..10287
                     /locus_tag="ACHW42_002006"
     CDS             9541..10287
                     /locus_tag="ACHW42_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR8943838.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10261..11226)
                     /locus_tag="ACHW42_002007"
     CDS             complement(10261..11226)
                     /locus_tag="ACHW42_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR8943839.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11223..12226)
                     /locus_tag="ACHW42_002008"
                     /pseudo
     CDS             complement(11223..12226)
                     /locus_tag="ACHW42_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12266..13318
                     /gene="fbaB"
                     /locus_tag="ACHW42_002009"
     CDS             12266..13318
                     /gene="fbaB"
                     /locus_tag="ACHW42_002009"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR8943840.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13626..14480
                     /gene="gatY"
                     /locus_tag="ACHW42_002010"
     CDS             13626..14480
                     /gene="gatY"
                     /locus_tag="ACHW42_002010"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR8943841.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14509..15771
                     /gene="gatZ"
                     /locus_tag="ACHW42_002011"
     CDS             14509..15771
                     /gene="gatZ"
                     /locus_tag="ACHW42_002011"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFR8943842.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15781..16233
                     /gene="gatA"
                     /locus_tag="ACHW42_002012"
     CDS             15781..16233
                     /gene="gatA"
                     /locus_tag="ACHW42_002012"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR8943843.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16264..16548
                     /gene="gatB"
                     /locus_tag="ACHW42_002013"
     CDS             16264..16548
                     /gene="gatB"
                     /locus_tag="ACHW42_002013"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR8943844.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16552..17907
                     /locus_tag="ACHW42_002014"
     CDS             16552..17907
                     /locus_tag="ACHW42_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR8943845.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4682 a         4100 c         4560 g         4600 t
ORIGIN      
        1 ttggttaatt tgttttaaat caataaaaca atatgtttac attgcggcag cttttttgat
       61 gtgagaaatt tgtttccaag agatacagac tcttaacaac caaaaataaa ttttattaag
      121 gaacatatta tgaaacgttc aattattgct gccgctgtct tttcttcttt ttttatgagc
      181 gctggagtat ttgctgcaga cgttgatacc ggaacattaa ccattaaagg aaatattgca
      241 gaatctccgt gtaaattcga agcgggtggt gattcagtaa gtattaatat gccgactgta
      301 ccaaccactg tctttgaagg taaagctaaa tattctacct atgatgatgc agtcggtgta
      361 accagcagca tgttaaaaat tagctgcccg aaagaagttg ctggtgtaaa actctcgttg
      421 atcacaaacg acaaaataac cggtaacgat aaggcgatag ccagtagcaa cgataccgta
      481 ggtgataaca gcgatgtcct agatgtttct gcacctttta acattgagag ttataaaaca
      541 gcggaaggtc aatatgctat tccgtttaaa gcaaaatacc tgaaactgac agataactca
      601 gtgcaatcag gtgatgtgtt atcttctctg gttatgcgtg tggcgcagga ttaataaata
      661 aaattgcaag tttctccagg taataaacat ggccgctatc cccatggcgg ctttttaatt
      721 taagaggcat taagatgaaa ggattattat ctttactcat tttttctatg gtccttcctg
      781 cacatgccgg aattgttatc tacgggacgc gcattattta cccggcagaa aataaagaag
      841 ttatggtgca gttgatgaac cagggaaatc gttcttcgct gctgcaggcg tggattgatg
      901 atggcgatac gtcgttacca ccagaaaaaa ttcaggttcc tttcatgtta acgccaccag
      961 tggcaaaaat aggggcaaat tccgggcagc aagtaaaaat caaaattatg ccgaataaac
     1021 tgcccactaa taaagaaagc attttttatc tgaatgttct gtatattcca ccaaatagtc
     1081 cagagcaaga aggcaagaat gcactgaagt ttgcgatgca aaacagaatt aagttgtttt
     1141 accggccagc gggtattgct ccggtaaata aagcgacctt taaaaagtta ctggtaaatc
     1201 gcagtggcaa tggattggtg ataaaaaatg actcagctaa ttgggtgacg atttcggatg
     1261 tcaaagctaa taatgttaaa gtcaattatg aaactattat gattgccccc ttagaaagtc
     1321 agagtgttaa tatcaaaagt aataatgcaa ataactggta tctgaccatt atcaatgacc
     1381 acggcaacta tattagtgac aaaatttaat tgcaggagct gcctatgttg agaatgaccc
     1441 cacttgcatc agcaatcgta gcgttattgc tcggcattga agcttatgca gctgaagaaa
     1501 cctttgatac ccattttatg ataggtggaa tgaaagacca gcaggttgca aatattcgtc
     1561 ttgatgataa tcaaccctta ccggggcagt atgacatcga tatttatgtc aataagcaat
     1621 ggcgcgggaa atatgagatt attgttaaag acaacccgca agaaacatgt ttatcaagag
     1681 aagttatcaa gcggttaggc attaatagcg ataacttcgc cagcggtaag caatgtttaa
     1741 catttgagca acttgttcag ggtgggagct atagctggga tatcggggtt tttcgtctcg
     1801 atttcagtgt cccgcaggct tgggtggaag aactggaaag tggctatgtt ccaccggaaa
     1861 actgggagcg gggtattaat gcgttttata cttcttatta tgtgagtcag tattacagcg
     1921 actataaagc gtcgggtaac aacaagagta catatgtacg ttttaacagc gggttaaatt
     1981 tactggagtg gcaactgcat tctgatgcca gtttcagtaa aacaaataac aatccagggg
     2041 tgtggaaaag caataccctg tatctggaac gtggatttgc ccaatttctc ggcacgcttc
     2101 gcgtgggtga tatgtacaca tcaagcgata tttttgattc tgttcgcttc agcggtgtgc
     2161 ggttgtttcg tgatatgcag atgttgccta actcgaaaca aaattttacg ccacgggtgc
     2221 aggggattgc tcagagtaac gcgctggtaa ctattgaaca gaatggtttt gtggtttatc
     2281 agaaagaggt tcctcctggc ccgtttgcga ttacagattt gcagttggcc ggtggtggag
     2341 cagatcttga tgttagcgtg aaagaggcgg acggctcggt aaccacctat ctggtgcctt
     2401 atgcagcggt gccaaatatg ctgcaacccg gcgtgtcgaa atatgatttt gcggcggggg
     2461 cgagcaagca aagtgatttt gtccaggcag gttatcagta tggttttaat aacttattga
     2521 cgctgtatgg cggcacgatg gttgctaata attactatgc gtttaccctc ggaacgggtt
     2581 ggaacacgcg cattggcgca atttccgtcg atgccacgaa gtcgcatagt aaacaagaca
     2641 acggcgatgt gtttgacggg caaagttatc aaattgccta caacaaattt gtgagccaaa
     2701 cgtcgacgcg ttttggtctg gcggcctggc gttattcgtc gcgtgattac cggacattta
     2761 acgatcacgt ttgggcaaac aataaagata attatcgccg tgatgaaaac gatatctatg
     2821 acattgccga ttattaccag aacgattttg gccgtaaaaa tagcttttct gccaatatga
     2881 gccagtcatt gccagaaggc tggggttctg tgtccttaag tacgttatgg cgagattact
     2941 gggggcgtag cggcagcagt aaggattatc agttgagtta ttccaataac tggcggcgga
     3001 taagctatac cctcgcggca agccaggcgt atggcgagaa tcatcatgaa gagaaacgtt
     3061 ttaatatttt tatatcaatt ccctgtgatt ggggtgatga cgttacgacg cctcgtcggc
     3121 aaatatatat gtctaactca acgacgtttg atgatcaggg ttttgcctca aataatacgg
     3181 gattatcagg aacagtaggg agtcgggatc agttcaatta tggtgtcaac ctgagtcatc
     3241 aacatcaggg aaatgaaacg acagctgggg cgaatttgac ctggaacgcg ccggttgcga
     3301 cagtgaatgg cagttatagt cagtcgagta cttatcgaca gactggagcc agtgtttcag
     3361 ggggcattgt cgcctggtcg ggtggcgtta atctggcgaa ccgtctttcc gaaacgtttg
     3421 ctgtgatgaa tgcgccagga attaaagatg cttatgtcaa tgggcaaaaa tatcgcacaa
     3481 caaaccgtaa tggagtggtg gtatacgacg gaatgacacc ttatcgggaa aatcacctga
     3541 tgctggatgt gtcgcaaagc gatagcgaag cagaattacg tggcaaccgg aaaattgccg
     3601 ccccttatcg cggcgcggtt gtactggtta attttgatac cgatcagcgc aagccctggt
     3661 ttataaaagc gttaagagcg gatggtcaac cattaacgtt tggttatgaa gtcaatgata
     3721 tccatggtca taatattggc gttgtcggcc agggaagcca gttatttatt cgcaccaatg
     3781 aaataccgcc atcggttaat gtagcaattg ataagcaaca aggactttca tgcacaatca
     3841 ccttcggtaa agagattgat gaaagtagaa attatatttg ccagtaatag tcaggtggtt
     3901 ctatggaaat tcgcataatg ctatttatat taatgatgat ggttatgcct gtgagctatg
     3961 cggcatgtta tagtgagtta tctgttcagc acaatttggt tgttcagggg aattttgcac
     4021 ttactcaaac acaaatagcg acatatgagc ataattttaa tgattcgtca tgcgtgagta
     4081 caaatactat cacccctatg agcccatcgg atattattgt tggactttat aacgatacca
     4141 ttaaattaaa tttacatttt gaatggacca ataaaaacaa catcacgttg tcaaataatc
     4201 agagcagttt caccagtggt tattcggtaa cggtgacacc tgcggccagt aatgcaaaag
     4261 tgaatatttc tgcggggggc ggtggttcag tgatgattaa tggtgttgcg acattatcca
     4321 gtgcttcatc atcgacacgc gggagtgccg cagtacaatt tctactgtgt ttattaggtg
     4381 gcaagtcatg gggtgcatgt gtaaatagct acagaaatgc attggcacaa aatgcaggtg
     4441 tctattcctt caatctgaca ttgtcataca acccgataac cacaacctgc aaacaggacg
     4501 atttattaat tactttagac agtattcccg ttttacaatt accagccaca ggtaacaaag
     4561 caacaataaa tagtaaaaaa agggatatta ttctgcgttg taaaaattta ttaggtcaac
     4621 aaaatcaaac atcacggaaa atgcaggtgt atttatcaag ttctgacttg ttaaccaaca
     4681 gcaacacaat actgaaaggt gcggaagata atgcgtagga tttattcttg aaagtaatgg
     4741 ttcgccagtc acacttttaa atatcactaa cagcagtaaa ggatacacaa atttaaaaga
     4801 aattgcggca aagtcaaaac ttacagatac aacggtttca attccgataa cagccagtta
     4861 ctacgtctac gatacaaaca aagttaaatc tggcgcactg gaggcaaccg cattaatcaa
     4921 cgtgaaatac gactaataac aagcaaaacg agcaagtggc taataataaa aaataacaag
     4981 gtgtctgcat ttccccctca tgatgagggg gcttttttta gcgatgataa aaaatctcac
     5041 cgtcgtaggc tttaatgatt ttaccgtcgg tgtcgctgat caacacgtac gcgccaccca
     5101 tataggtcca gtgcgtcccg gcatcaggcg cgggcaggtt acgtaactgc cactgcttaa
     5161 tgttgtactc atcggtgcgg tacatctctg ggacggtatc acctggtttg aaatgcttaa
     5221 agtcagcaat gaaactactt aactcatact tagaaattcc cgaggtattg gtcgaacccg
     5281 cggtggctgg tgcggcccag gcggcactgg taaccagcaa aagcacaccc agcaacatct
     5341 tatttttaat ggtcataaac tctccacttt ggggatctgt aatatcagtc gcatatcttt
     5401 cctgtcatac aatataactg acaagcgata ttttgttagt gatcccatga tttcgtaaaa
     5461 agtgtaaatg aaaggatgcc ctaaaagcat ccttagcgag cagagagtca gcggaaatag
     5521 ctgatttaag aatcatcctg ggctgaagat aacatgcaag cctaaaggat tgagttatcg
     5581 cattatgccc atgaagccat gtacgcccat atacacaccg actaagccaa tcaacagact
     5641 ggaaaaatag ggggcgcgtt tagcgagagt gttaaatccg ctccagcgtt ttgcgacctg
     5701 ctgaacgctg attgctgcgc caacgcctac ggtgacaagc gttaacgcca ggccaatgct
     5761 gaaactgacg accagtgttg cgcccagtgt cagggctttc aactgaatgc atatcaacag
     5821 cacggtaatt gctgccgggc aggggataaa gccaccggtt aagccaaata acaaaatttg
     5881 ccagttgatg acctctctac catcaaagcg tcgtttaatg tcattggcat gggctcgtgc
     5941 atgggcatcc tgatactcgc catgttcgtg atggtgatga tgtccttggt cgtggtgatg
     6001 ttcgtgatcg tgatgatggt gatgatgctc ataatcatgc ccgtgcatat tctccagcca
     6061 gttgcgttcg ccgcgctagg tacgccagaa catccagaac gcggtgccaa taatgatcac
     6121 tgcggaaatc agctggagcc acggttctgc tgattgagca gtaaagcgct tactgatcac
     6181 catcccgcca aaggcaatca accagaccac cgcggtatgc gaaatggttg ctgccagtcc
     6241 gagcatcacc gcctgtttaa tggtgccttt gatggcgatg ataaacgccg ccatcatcgt
     6301 ttttgagtgc cctggttcca ggccatgaag cgcaccaagt aagatggcgc tggggatgaa
     6361 gaaccaggcg tttccttgct gaagaagagt tgtaaattcg gtcatgataa taattcttag
     6421 tattaattcg gcaatctgat tctactcccc cccagtacct gatactaccc ccccagtaga
     6481 ttaatagtgc tatgattttt catattcttg ttaaccaggt gttgccatgt ctcatacaat
     6541 ccgtgataaa cagaaactga aagcgcgtgc cagtaagatt cagggccagg tcgtggcgct
     6601 caagaaaatg ctcgacgagc cgcacgaatg cgctgcagtt ttacaacaga ttgctgctat
     6661 ccgtggagcg gtaaacggtc tgatgcggga agtgattaaa ggtcatctga cggaacacat
     6721 cgttcaccag ggggatgagc taaaacgtga agaagatctg gatgtcgttc tgaaggtgct
     6781 ggattcatat atcaaataat ttattaacgc gattgtaaaa ctgccgtttt tcctcgttta
     6841 caacgggggc gctggacatt accatcctcc tctgcgattt atcatcgcaa ccaaacgact
     6901 cggggtgccc ttctgcgtga aggctgagaa atacccgtat cacctgatct ggataatgcc
     6961 agcgtaggga agtcacggac caccaggtca ttgcttcttc acgttatggc aggagcaaac
     7021 tatgcaagtc gacctgctga gttcagcgca atctgcgcac gcgttacacc tttttcacca
     7081 acattcccct cttgtgcact gcatgaccaa tgatgtggtg caaaccttta ccgccaatac
     7141 cttgctggcg ctcggtgcat cgccagcgat ggttatcgaa accgaagagg ccagtcagtt
     7201 tgcggctatc gccagtgcct tgttgattaa cgttggcaca ctgacgcagc cacgcgcaca
     7261 ggcgatgcgt gctgccgttg agcaagcaaa aagctctcaa acacgctgga cgcttgatcc
     7321 agtagcggtt ggtgcgctcg attatcgccg ccatttttgt catgaacttt tatcttttaa
     7381 accggcagcg atacgtggta atgcttcgga aatcatggca ttagctggca ttgctaatgg
     7441 cggacgggga gtggatacca ctgacgccgc agctaacgcg atacccgctg cacaaacact
     7501 ggcacgggaa actggcgcaa tcgtcgtggt cactggcgag gtggattatg ttaccgatgg
     7561 acatcgtatc attggtattc acggtggcga tccgttaatg accaaagtgg taggaactgg
     7621 ctgtgcatta tcggcggttg tcgctgcctg ctgtgcgtta ccaggcgata cgctggaaaa
     7681 tgtcgcatct gcctgtcact ggatgaaaca agccggagaa cgcgcagtcg ccagaagcga
     7741 ggggccaggc agttttgttc cacatttcct tgatgcgctc tggcaattga cgcaggaggt
     7801 gcaggcatga aacgaattaa cgctctgacg attgccggta ctgatccgag tggtggtgcg
     7861 gggattcagg ccgatcttaa aaccttctcg gcacttggcg cttatggttg ctcagttatt
     7921 actgcactgg tggcgcaaaa tacccgtggc gtacagtcgg tgtatcgcat tgagcctgat
     7981 tttgtcgccg cccagctcga ttcggtgttc agcgatgtgc gaatcgatac cactaaaatc
     8041 ggtatgttgg cggaaaccga tattgttgaa gcggtggcag aacggttgca acgttatcag
     8101 atccaaaacg tggtactcga caccgttatg ctggcaaaaa gcggcgaccc gctgctttca
     8161 ccttcggcgg ttgctacgct gcgcagtcga ttattgccac aggtttcatt aataacgcca
     8221 aacttgcccg aagctgccgc cttgctcgac gcgccacacg cgcgcaccga acaggaaatg
     8281 ctggaacaag ggcgatcgct gttggcgatg ggctgtggcg cagtgctaat gaaaggtggt
     8341 catctggatg atgagcaaag cccggactgg ctgtttaccc gcgagggtga acaacggttt
     8401 accgcaccgc gcattatgac caaaaacacc cacggcactg gttgtacact ctctgcggcg
     8461 ttggctgcac tacgcccgcg ccatacaaac tgggctgaca ccgtacagga ggcaaaaatc
     8521 tggctttcat cggcgttagc ccaggccgac acgctggaag ttggtcacgg tattggtccg
     8581 gttcaccact tccacgcctg gtggtgagca ccgtactata gctgctttgg ttattgtcag
     8641 cctttggcac tgacattcag ggatgaaacc aatgcagcta agaattacca gtcgaaaaaa
     8701 actcaccgct ctattatgcg ctcttgggct aatttccatc gttgcaattt atccgcgtca
     8761 gacggtgaat tttttctatt cgacagcagt tcagattaca gactacatcc acttctacgg
     8821 ttatcgcccg gttaaatctt tcgctattcg cattcctgcc agttacacca ttcacggatt
     8881 agatgtttcg cgctggcagg agcgtatcga ctggcagcgg gtggcaaaaa tgcgcgacaa
     8941 cgatatccgc ttacagtttg cttttattaa ggctacggaa ggcgaaaagc tggtggaccc
     9001 ctatttttcg cgtaactggc aattaagccg cgaaaatggc ctgctgcgcg gggcgtatca
     9061 ttatttctcc ccgtcggtat ccgcttcagt tcaggcgcga ttatttctgc aaacggtgga
     9121 tttctcacaa ggcgatttcc ctgccgtgct ggacgtagaa gaacggggaa aattatcggc
     9181 aaaagaatta cgcaagcggg taagtcagtg gctaaaaatg gtcgaaaaac gcacggaaaa
     9241 aaagccgatt atttactcag gagccgtttt ttatcacacc aatctggcgg gctatttcaa
     9301 tgaatatccg tggtgggtgg ctcactatta tcaacgtcgt ccggacaatg acggcatggc
     9361 ctggcgcttc tggcagcatt ccgaccgtgg acaggtagat ggcatcaatg gtccggtgga
     9421 ttttaatgtg tttaatggca cggggatgag ttgcaggcat tcgttgatgg gattaaagaa
     9481 acgccttaaa taagaagcag tgatatactc ccaccagact ccctggcagg aaaaagacaa
     9541 atggaacaag cgcataccca gctgattgcc caactgaacg agcgtatttt agcggcggat
     9601 aacacgccgc tttatattaa gtttgccgaa acggtaaaaa atgccgtgcg cagcggggtg
     9661 ctggagcatg gcaatatttt gcccggtgag cgtgatttaa gtcagttaac cggcgtttcg
     9721 cgcattacgg tgcgcaaggc gatgcaggcg ctggaagaag agggcgtggt gacgcgttcg
     9781 cgcggttacg gcacgcagat caacaacatc ttcgaatact cgttgaaaga agcgagaggt
     9841 ttttctcagc aggtggtatt gcgcgggaaa aagcccgata cgctatgggt taataagcgt
     9901 gtcgtgaaat gtcccgaaga agttgcgcag cagctggcgg ccgaagcggg aagtgatgtc
     9961 tttttgctta agcgtattcg ctatgtcgat gaagaagcgg tatcgattga ggaatcgtgg
    10021 gttccggcgc atttaattca tgatgttgat gccatcggaa tttctcttta tgactatttc
    10081 cgcagccagc acatttaccc acaacgtacg cgttcccgcg ttagcgcccg gatgccggat
    10141 gccgagtttc agtcacatat tcagttagat agcaaaatac cggtgctggt gatcaagcaa
    10201 gtggcgcttg accaacagca acggcctatt gagtacagca ttagccactg tcgcagcgat
    10261 ctatacgttt ttgtgtgcga ggagtagttc ctcgcgcgtt ggcgcacaat caccgccccg
    10321 atgcccgaca acccacgacg ccactgcatt gcccagtaat acggcatccg ccagtggcag
    10381 accagaggcc agcccggcaa gtacgccacc tgcatgactg tcgcccgccc caatggtgtc
    10441 tacaacttgc gttggaaatg ccggaatgca gccagaagcg tcgttgctga aataccatgc
    10501 gccttcttta tcgagacgaa cgatcaacgg cgcggcaaat ttctcctgcc attgcttgcc
    10561 aagtgttgtt atctctgcgg ataaagcaaa acgttcggcg gcaatctcag cctcttgacg
    10621 attgagtgac actaaaggtc gacaggccat gatccgcgcc agtaatgcgt ccgggatatc
    10681 gccaatacgt gggcaaaaat cgataaacgg cgtcacgtct tgcagctttt ccagccattc
    10741 cactaacaat tcgccgcagg gcgaggccag ttgataaccg gaaaaataga gcaggttgcc
    10801 aggcgcaacg gttaatcgtg ccaaccattg gcgattccat tgattttcca caccgctgaa
    10861 tgacataaaa gtgcgttcgc catccggctc aaccagtgcc agacaccagc cgttatcgct
    10921 ttcggcgtta tcgatcaggc tgattaaccc ctctttcgcc atccggttgc gaataatctc
    10981 cgcccacacg ccctgaccga gcggcaaggc attacccgct tcgatgccga ggcgctttaa
    11041 cgccacggca atattcagtg cgcagccgcc aacgttaacg ctctgctgtt tcagttcgat
    11101 atcgcaccca cgccagggga gggcataagc gtcggcgatc acgtcaatga ccgccgcgcc
    11161 gacgaccatc accggctgac gcgtggttaa ttcaggcagc agtgtgtgta atcgagcgcc
    11221 gctcataccg cctcccgttg ttgacgatat ttcgccagcg ctgtggcata gcggttgaaa
    11281 tcaagctgat ttaccgcatc cagctccgcc tttaacgcag gatcgacagc gttaatgcca
    11341 tgcaacgcgc cgcaaatcgc cgtcgccata gcacctatgg tgtctgtgtc gccgccaaga
    11401 ttggcgcaca ggacggcgca gcggtttggg tcggtttgtg ccagttcaac tagcgcaatg
    11461 gcgcacggaa cggactcaat agtgctggtg cctgcgccaa cgacctggta aagctgttcg
    11521 cgggcggatt cggtgccgtc ggcattacgc acaattttca gcgcaatctc cagacgtgcc
    11581 gccagtgagg cgctgaacgt ggtggtgcgt ttttgctgtg catgtcgcgc aattgaaggc
    11641 aatgaatcaa caatcgctga ccagcgttct ccgtcaatgg cacgagaaat cgcccatgcg
    11701 atgactaccg cgcctgcaac cgccagatcg gatttatggg tcgggctgga cgccagcgcc
    11761 acatcatcaa taaaggaatc aacatcacgc gctggaagca aacaacctaa tggcgagacg
    11821 cgcatcgctg cgccgttggt tacgccgttg ttttccagtt cagcaacggg tttaccgtcg
    11881 cgaatggcgt taagcgcaat cttcgaggtc ggacctagta cgtttttgtt aaaggcgtcg
    11941 aaacgcaacg cccagtcgag aatattacgc ccaatcagat tcggatcgat cttgccttca
    12001 cattccagta acgcatccgc cagacacagg ccatcgaggt atcgtcggtg aattcggcgc
    12061 ggttaaaata acaggccgcg ttattctcct ttggtccagg cagaaaacgg tcaatccagc
    12121 caaagtgtgc tttaacgcgg ctgcgtggcc aaagctcgga gggcatcccc atcgcatccc
    12181 ctaacgcctg cccataaaga gcaccgagaa tacgttctgt tttcatttaa cttccccttg
    12241 aatcggaatt tacgggagca tagtaatgac agatattgcg cagttgcttg gcaaagacgc
    12301 cgacaacctt ttacagcacc gttgtatgac tattccttct gaccagcttt atctccccgg
    12361 acatgactac gtagaccgcg tgatgattga caataatcgc ccgccagcgg tgttacgtaa
    12421 tatgcagacg ttgtacaaca ccgggcgtct ggctggcaca ggatatcttt ctattctgcc
    12481 ggttgaccag ggcgttgagc actctgccgg agcttcattt gctgctaacc cgctctactt
    12541 tgacccgaaa aacattgttg aactggcgat cgaagcgggc tgtaactgtg tggcatcaac
    12601 ttacggcgtg ttggtgtcgg tatcgcggcg ctatgcgcat cgcattccat ttctcgtcaa
    12661 acttaatcac aacgagacgc taagttaccc gaatacctac gatcaaacgc tgtatgccag
    12721 cgtggagcag gccttcaaca tgggcgcggt ggcggttggt gcgactatct attttggctc
    12781 ggaagagtca cgtcgccaga ttgaagaaat ttctgcggcc tttgaacgcg cgcacgagct
    12841 gggcatggtg acagtgctgt gggcctattt gcgaaactcc gcctttaaga aagatggcgt
    12901 tgattaccat gtttccgccg acctgactgg tcaggcgaac catctggcgg caaccatagg
    12961 tgcagatatc gtcaaacaaa aaatggcgga aaataacggc ggctataaag cgattaatta
    13021 cggttatacc gacgatcgcg tgtacagcaa gttaaccagc gaaaacccga ttgatctggt
    13081 gcgttatcag ttagctaact gctatatggg ccgggctggg ttgataaact ccggcggtgc
    13141 tgcaggcggt gaaactgacc tcagcgatgc agtgcgtact gcggttatca acaaacgcgc
    13201 aggcggaatg gggctgattc ttggacgtaa ggcgttcaag aaatcgatgg ctgacggcgt
    13261 gaaactgatt aatgccgtgc aggacgttta tctcgatagc aaaattacta ttgcctgata
    13321 cgcctcatac gaacagcacc accccgtagg acggataaga cgtttacact gcatccggca
    13381 acctatgcct gatgcgacgc ggaagcgtct tatcatgcct acatagcact gccacgtatg
    13441 tttacaccgc atccggcata aaaacacgcg cactttgcta cggcttccct atcgggaggc
    13501 cgtttttttg cctttcactc ctcgaacaat tttcatattg ttgtttttgc gatcattatc
    13561 tcgacattta aaagcaaata atttcattat attttgaaat cgaaagcaaa cgacaagata
    13621 tgaaaatgta cgtggtatcg acaaagcaga tgctgaacaa cgcacagcgc ggcggttatg
    13681 cggttccggc attcaatatt cacaatctcg aaacgatgca agtggtggtg gaaaccgctg
    13741 ccaacctgca tgcgccggtc atcatcgccg gaacgcctgg cacatttact catgctggta
    13801 cagaaaatct gttggcgctg gtcagcgcga tggcgaagca atatcaccat ccactggcaa
    13861 ttcatctcga ccatcacacg aaatttgacg atatcgctca gaaggttcgt tctggcgtgc
    13921 gctcagtcat gattgacgcc tcgcatttgc cttttgcgca aaatatttca cgggtcaaag
    13981 aggtggtgga tttttgccat cgctttgatg tcagcgtcga ggcggagctg gggcaacttg
    14041 gcggccagga agatgatgtg caagtcaatg aagtcgatgc gttgtacacc aaccccgcac
    14101 aggcgcgtga atttgccgag gcaaccggga ttgattccct ggcggtcgcc atcggcacgg
    14161 ctcacggtat gtatgccagc gcaccggtgc ttgatttttc tcgactggag aacattcgcc
    14221 agtgggtgaa cttaccgctg gtgctgcatg gcgcgtcagg attatcgact aaggatattc
    14281 agcaaaccat caaactgggg atatgcaaaa tcaacgttgc aacggagctg aaaaatgcct
    14341 tcttgcagtc gttaaaaaat tacctgaccg agcaccccga agcgaccgat ccccgggatt
    14401 atttgcagtc ggctaaatcc gcaatgcgcg atgtggtgag caaagtgatt gccgattgtg
    14461 gctgcgaggg cagggcataa cgcacctgcc atttaacaag gaaaaaacat gaaaacgtta
    14521 attgcccggc ataaagctgg tgaacatatc ggcatatgtt cagtctgttc tgcccatccg
    14581 ttggttatcg aagcggcgct ggcatttgat cgcaacagca cgcgcaaagt gctgattgaa
    14641 gcaacgtcaa accaggtcaa tcaatttggc ggttataccg gaatgacacc ggcagacttt
    14701 cgcgaatttg tttttacgat tgccgataaa gttgggtttg cacgcgaacg cattattctc
    14761 ggcggcgatc atctgggacc aaactgctgg cagcaagaaa atgcggatgc ggcgatggaa
    14821 aaatccgtcg agctggtaaa agcatatgtt cgtgctggct tcagtaaaat tcatcttgat
    14881 gcgtcaatgt cctgcgcggg ggataccata ccgttagcac cagaaacggt tgcggaacga
    14941 gctgctgtgc tttgctttgc tgcggaaagt gtggcgacag attgccagcg tgagcaactg
    15001 agctatgtca ttggcactga agttccggtt ccgggcggtg aggccagcgc cattcagtca
    15061 gtacacatca cccatgttga agatgccgcc aatactttac gtacgcatca aaaggccttt
    15121 attgcccgtg ggctgacaga ggcgttaaca cgtgtgattg ccatcgtggt gcagccgggt
    15181 gtggaatttg atcacagcaa tattatccat tatcagccgc aggaagcgca ggcactggcg
    15241 caatggatag aaaacacccg aatggtttat gaagcacatt ctaccgatta ccagacccgg
    15301 acggcttatt gggaattagt ccgcgatcac tttgcaatat tgaaagtcgg tcccgcatta
    15361 acctttgctt tacgcgaggc gatatttgca ctggcacaaa ttgagcagga acttatcgcc
    15421 cccgaaaatc gcagcggttg cctggcggta attgaagaag tgatgctcga cgaaccgcaa
    15481 tactggaaaa aatattatcg cacgggtttt aacgattcat tactggatat tcgttacagc
    15541 ctgtcggatc gtattcgtta ttactggccg catagtcgga ttaaaaatag cgttgaaacg
    15601 atgatggtga atctgcaagg cgtggacatc ccactgggca tgattagtca gtatcttccc
    15661 aaacaatttg aacgcattca gtccggggaa ttatcagcaa tgccgcatca gcttattatg
    15721 aataaaattt atgatgtttt gcgcgcctat cgctacggct gtgcggaata aggacggtat
    15781 atgactaacc tgtttgttcg tagcggaatt tcttttgtcg atcgtagcga agttttaacc
    15841 tatatcggta atgagatgct cgcgaaaggt gtggttcatg atacctggcc acaggcatta
    15901 attgccagag aagcagaatt ccctaccggg ataatgcttg agcagcacgc cattgcaata
    15961 ccgcattgtg aggcgattca tgctaagtcg tcagccattt atctgttaag gccaacaaat
    16021 aaagttcatt ttcagcaagc ggatgatgat aacgacgtgg cggtatcgtt ggttattgcg
    16081 ttgattgtgg aaaatccgca gcagcaattg aaacttttac gctgtttatt tggcaagtta
    16141 caacagcccg atatcgtcga gacactaatt actcttcctg aaactcagtt aaaggaatac
    16201 ttcacaaagt atgttttaga ttcagacgaa taaatccctc tgtaacaata aaaaggacta
    16261 tttatgaaac gcaagattat tgtcgcttgc ggaggcgcgg ttgcgacctc tacgatggcg
    16321 gcggaagaaa ttaaagagtt gtgtcagagt cataatattc ctgttgaatt aatccagtgt
    16381 cgggttaatg aaatagaaac ctatatggat ggtgtgcatt tgatatgtac cactgccagg
    16441 gtggatcgta gttttggcga tattccgtta gttcacggca tgccttttgt ttctggtgtc
    16501 ggtatcgaag cattacaaaa taaaattctg actatcttac agggatgacc tatgttttca
    16561 gaagtcatgc gttatattct cgacctcggc cctacagtga tgctgccggt tgtcatcatt
    16621 attttttcta aaatattagg catgaaggca ggcgattgct ttaaagcggg tctgcatatc
    16681 gggattggct ttgttggcat tggccttgtg attggcttaa tgctggattc cattggtccg
    16741 gcggcgaaag cgatggcgga aaatttcgac ctgaatctgc atgtggtcga tgtcggctgg
    16801 ccgggctctt caccaatgac ctgggcgtcg caaattgcgc tggtggcgat tccgattgcg
    16861 atattggtta acgtggcgat gttactgacc cgtatgacgc gggtggtaaa tgttgatatc
    16921 tggaatatct ggcatatgac cttcaccggc gcgttgctgc acctggcaac cggttcatgg
    16981 atgataggga tggcgggtgt ggtaattcac gcggcgtttg tttataagct cggcgactgg
    17041 tttgcccgcg atacccgaaa tttctttgag ctggaaggta ttgctattcc gcacggtacg
    17101 tcggcgtata tggggccgat tgcggtgctg gtcgatgcta tcatcgagaa aatcccaggc
    17161 gttaaccgaa ttaaatttag cgccgacgat attcagcgca aatttggccc ttttggcgaa
    17221 cctgtcaccg tgggttttgt gatggggctg attatcggca tcctcgcggg ttacgatgtc
    17281 aaaggtgtat tgcagctggc ggtaaaaacg gcggcagtta tgctgctgat gccacggatg
    17341 attaaaccca tcatggatgg tttaacgccc atcgctaagc aggcccgtag tcgtttacag
    17401 gcgaagttcg gcggtcagga gttcctgatt ggcctggatc ccgcattact gctggggcat
    17461 acggcggtgg tatcggcaag cctgattttt attccgctca ccattttaat tgctgtttgt
    17521 gttccgggta atcaggtact gccgtttggc gatcttgcca ctatcggctt cttcgtggcg
    17581 atggcggtcg cggtgcatcg tggaaatctg ttccgcacct taatctcggg tgtcatcatt
    17641 atgagcatca ccctgtggat cgcgacgcaa actattggtt tgcacaccca accggcggct
    17701 aatgctgggg cgttaaaagc cgggggtatg gtggcttcaa tggatcaggg cggttctccc
    17761 attacctggt tactgattca ggttttctcc ccgcaaaata ttcccggttt cattattatc
    17821 ggcgcaattt atctgaccgg tattttcatg acctggcgta gagcgcgtgg ttttactaaa
    17881 caagagaaag ccgttctcgc agaataattt ttacctgagg gggaggttat cccccttaac
    17941 aa
//
LOCUS       DAZSNM010000072        17736 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZSNM010000072 DAZSNM010000000
VERSION     DAZSNM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17736
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.072"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..574
                     /locus_tag="ACHW42_002015"
     CDS             <1..574
                     /locus_tag="ACHW42_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8943846.1"
                     /translation="AEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADVQNEWFGLCM
                     DLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLDN
                     LKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEKG
                     SDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /gene="ybhA"
                     /locus_tag="ACHW42_002016"
     CDS             810..1628
                     /gene="ybhA"
                     /locus_tag="ACHW42_002016"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFR8943847.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="ACHW42_002017"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="ACHW42_002017"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR8943848.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="ACHW42_002018"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="ACHW42_002018"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR8943849.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="ACHW42_002019"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="ACHW42_002019"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8943850.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="ACHW42_002020"
     CDS             complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="ACHW42_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR8943851.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="ACHW42_002021"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="ACHW42_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR8943852.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="ACHW42_002022"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="ACHW42_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR8943853.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="ACHW42_002023"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="ACHW42_002023"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR8943854.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="ACHW42_002024"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="ACHW42_002024"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR8943855.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="ACHW42_002025"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="ACHW42_002025"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR8943856.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="ACHW42_002026"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="ACHW42_002026"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR8943857.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="ACHW42_002027"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="ACHW42_002027"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR8943858.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="ACHW42_002028"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="ACHW42_002028"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR8943859.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="ACHW42_002029"
     CDS             13934..14314
                     /locus_tag="ACHW42_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR8943860.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="ACHW42_002030"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="ACHW42_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR8943861.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="ACHW42_002031"
     CDS             complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="ACHW42_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR8943862.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="ACHW42_002032"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="ACHW42_002032"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR8943863.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17415..17490)
                     /locus_tag="ACHW42_002033"
     tRNA            complement(17415..17490)
                     /locus_tag="ACHW42_002033"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17455..17457),aa:Lys,seq:ttt)
     gene            complement(17494..17569)
                     /locus_tag="ACHW42_002034"
     tRNA            complement(17494..17569)
                     /locus_tag="ACHW42_002034"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17534..17536),aa:Val,seq:tac)
     gene            complement(17621..17696)
                     /locus_tag="ACHW42_002035"
     tRNA            complement(17621..17696)
                     /locus_tag="ACHW42_002035"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17661..17663),aa:Lys,seq:ttt)
BASE COUNT         4521 a         4637 c         4409 g         4169 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcaaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gagcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtttgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatcccg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gtgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag
    12841 ctggcagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg
    17401 gcgctttttt gcattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
    17461 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
    17521 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
    17581 ggatactaca aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc
    17641 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg
    17701 gtgggtgatg acgggatcga accgccgacc ccctcc
//
LOCUS       DAZSNM010000073        17666 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZSNM010000073 DAZSNM010000000
VERSION     DAZSNM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17666
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.073"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            397..1011
                     /gene="evgA"
                     /locus_tag="ACHW42_002036"
     CDS             397..1011
                     /gene="evgA"
                     /locus_tag="ACHW42_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR8943864.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            1016..4609
                     /gene="evgS"
                     /locus_tag="ACHW42_002037"
     CDS             1016..4609
                     /gene="evgS"
                     /locus_tag="ACHW42_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR8943865.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4665..5810)
                     /gene="yfdE"
                     /locus_tag="ACHW42_002038"
     CDS             complement(4665..5810)
                     /gene="yfdE"
                     /locus_tag="ACHW42_002038"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR8943866.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5884..6828)
                     /gene="yfdV"
                     /locus_tag="ACHW42_002039"
     CDS             complement(5884..6828)
                     /gene="yfdV"
                     /locus_tag="ACHW42_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR8943867.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6898..8592)
                     /gene="oxc"
                     /locus_tag="ACHW42_002040"
     CDS             complement(6898..8592)
                     /gene="oxc"
                     /locus_tag="ACHW42_002040"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR8943868.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8646..9896)
                     /gene="frc"
                     /locus_tag="ACHW42_002041"
     CDS             complement(8646..9896)
                     /gene="frc"
                     /locus_tag="ACHW42_002041"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR8943869.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10409..11032)
                     /locus_tag="ACHW42_002042"
     CDS             complement(10409..11032)
                     /locus_tag="ACHW42_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR8943870.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            11329..11604
                     /gene="ypdI"
                     /locus_tag="ACHW42_002043"
     CDS             11329..11604
                     /gene="ypdI"
                     /locus_tag="ACHW42_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR8943871.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(11681..11923)
                     /locus_tag="ACHW42_002044"
     CDS             complement(11681..11923)
                     /locus_tag="ACHW42_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR8943872.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            12276..13196
                     /gene="lpxP"
                     /locus_tag="ACHW42_002045"
     CDS             12276..13196
                     /gene="lpxP"
                     /locus_tag="ACHW42_002045"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR8943873.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            13551..13622
                     /gene="ypdK"
                     /locus_tag="ACHW42_002046"
     CDS             13551..13622
                     /gene="ypdK"
                     /locus_tag="ACHW42_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR8943874.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(13687..14925)
                     /gene="alaC"
                     /locus_tag="ACHW42_002047"
     CDS             complement(13687..14925)
                     /gene="alaC"
                     /locus_tag="ACHW42_002047"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR8943875.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            15302..16788
                     /locus_tag="ACHW42_002048"
                     /pseudo
     CDS             15302..16788
                     /locus_tag="ACHW42_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
     gene            16803..17537
                     /gene="ypdB"
                     /locus_tag="ACHW42_002049"
     CDS             16803..17537
                     /gene="ypdB"
                     /locus_tag="ACHW42_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR8943876.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            17550..>17666
                     /locus_tag="ACHW42_002050"
     CDS             17550..>17666
                     /locus_tag="ACHW42_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8943877.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASL"
BASE COUNT         4982 a         4113 c         3810 g         4761 t
ORIGIN      
        1 tagatgaaat ttatgaattg aatgaaaaga gtattcattc ttcatatcaa acacagtgca
       61 tgtgttgcac attatcccag gaacgttgga ggcctaattt aaatgaggtg caaaaaaaac
      121 aacagaggac taaaccgtgg cttttgcaat acaattctta cgcctgtagg attagtgaga
      181 agacttatag tgctaacttg aaactataaa tcatcggtat aatccctgat tttattgttg
      241 acatttcatt tatgccgact atttatatgg tatacttgtc gaattatctt aaaggaaact
      301 cagattttct tatttttatt gagaaaatga gatgacgcct tatgtctgta ttactacagg
      361 gagaagggag atgcttcatt gcaaagggaa taatctatga acgcaataat tattgatgac
      421 catcctcttg ctatcgcagc aattcgtaat ttattgatca aaaacgatat tgaaatctta
      481 gcggagttaa ctgaaggcgg aagcgccgtt cagcgggtgg aaacacttaa gcctgatatc
      541 gtcatcattg atgtcgatat ccccggagtt aacggtatcc aggtgttaga aacgctgagg
      601 aagcgccaat atagcggaat tattattatc gtctccgcta aaaatgacca tttttacggg
      661 aaacattgtg ctgatgctgg cgctaatggt ttcgtgagta aaaaagaagg catgaacaat
      721 atcattgcgg ctattgaagc tgcaaaaaat ggctactgct atttcccctt ctctctcaac
      781 cggtttgttg gaagtttaac gtccgaccag caaaaactcg actccttatc gaaacaagaa
      841 attagtgtca tgcggtatat tcttgatggc aaggataata atgacattgc ggaaaaaatg
      901 ttcatcagca acaaaactgt cagcacgtat aaaagtcgcc tgatggaaaa attagaatgt
      961 aaatcactga tggatcttta cacattcgcg caacgtaaca aaatcggcta atcacatgaa
     1021 gtttttaccc tatttttttc ttctctgttg tggtctttgg tcgaccataa gtttcgcaga
     1081 cgaagattac atcgaatatc gtggcatcag tagtaacaac cgtgtcacac ttgatccagt
     1141 acgtctgagc aacaaggaat tacgttggtt agcgagcaaa aaaaatcttg tgattgcagt
     1201 acacaagtcc caaacggcta ccttgttgca taccgattcg cagcaacagg ttcgtggtat
     1261 taatgctgat tatttaaatc ttttaaaaag agcgttaaat atcaaattaa cactccggga
     1321 atacgcagat catcaaaaag caatggacgc gcttgcagaa ggtgaagtcg atatagtgtt
     1381 atcacattta gttacttcgc cgcctcttaa taatgacatt gctgcaacca aaccattgat
     1441 aattaccttt ccggcgctgg taaccaccct tcacgactca atgcgaccgc ttacctcacc
     1501 aaaaccagta aatattgctc gggtagcaaa ttacccccca gacgaggtaa ttcatcaatc
     1561 atttccaaaa gcaacaatta tctcttttac aaatttatat caggcattag catccgtctc
     1621 agctgggcac aatgattact ttattggtag taacatcatt accagcagta tgatttcccg
     1681 ctatttcact cactccttaa atgtagtgaa atattataac tcgccgcgtc aatataattt
     1741 tttcttgacc agaaaagaat ctgtcattct taatgaagta ctcaatagat ttgttgatgc
     1801 tttaacaaat gaagttcgct atgaagtatc acaaaattgg cttgatacag gaaacctggc
     1861 ctttctgaac aaaccattag aactcactga acatgaaaaa cagtggatta agcagcatcc
     1921 caatttaaag gtgctggaaa atccttactc gcccccctat tctatgacgg atgaaaatgg
     1981 ctcggttcgg ggcgttatgg gggacattct taatattatt accttgcaaa caggtttaaa
     2041 tttttctccg atcaccgttt cacacaatat ccatgctgga acacaactta gccccggagg
     2101 atgggatata atacctggcg ctatttatag tgaagatcga gaaaataatg ttttatttgc
     2161 tgaagccttc ataacaacgc cttacgtttt tgtcatgcaa aaagcgcctg acagtgaaca
     2221 aacattaaaa aaaggaatga aagttgccat tccatattat tatgagctgc attcgcaatt
     2281 aaaagagatg tatccggagg ttgaatggat acaggtcgat aatgccagcg ctgcatttca
     2341 caagattaag gaaggtgaac ttgatgctct ggtcgcgaca cagctaaatt cgcgttacat
     2401 gatcgatcat tactatccta atgaacttta tcattttctt attcctggcg ttccgaatgc
     2461 atcgctttcg ttcgcttttc ctcgcggaga accggaactt aaggatatta ttaataaagc
     2521 actgaatgca attcccccaa gcgaagttct gcgcctgacg gaaaaatgga ttaaaatgcc
     2581 caatgtgacc attgacacat gggacctata tagcgagcaa ttttatattg ttacgacatt
     2641 atccgtttta ttagttggca gtagcctttt atggggattc tacctgttac gctcagttcg
     2701 tcgtcgtaaa gtcattcagg gtgatttaga aaaccaaata tcattccgaa aagcactctc
     2761 ggattcctta ccgaatccaa cttatgttgt aaactggcaa ggtaatgtca ttagtcataa
     2821 tagtgctttt gaacattatt tcactgcgga ttactacaaa aatgcaatgt taccattaga
     2881 aaacagtgat tcacccttta aagatgtttt ttctaatgcg catgaagtca cagcagaaac
     2941 gaaagaaaat cgaacaatat acacacaggt atttgaaatt gataatggca tcgagaaaag
     3001 atgcattaat cactggcata cattatgcaa tcttcctgca agtgacaatg cagtatatat
     3061 ttgtggttgg caagatatta ctgaaacgcg tgatctaatt aatgcactcg aggtagaaaa
     3121 aaataaagcg ataaaggcta ccgtagcaaa aagtcagttt ctggcaacga tgagtcacga
     3181 aataagaaca ccaataagct ctattatggg cttcctggaa cttctgtcgg gttctggtct
     3241 tagcaaggag caacgggtgg aggcgatttc acttgcctac gccaccggac aatcactcct
     3301 cggcttaatt ggtgaaatcc ttgatgtcga caaaattgaa tcgggtaact atcaacttca
     3361 accacaatgg gtcgatatcc ctactttagt ccagaacact tgtcactctt tcggtgcgat
     3421 tgctgcaagc aaatcgatcg cattaagttg cagcagtaca tttcctgatc attacctggt
     3481 taagatcgac cctcaggcgt ttaagcaggt cttatcaaat ttactgagta atgctctcaa
     3541 atttaccacc gagggggcag taaaaattac gacctccctg ggtcacattg atgacaacca
     3601 cgctgttatc aaaatgacga ttatggattc tggaagtgga ttatcgcagg aagaacaaca
     3661 acaactgttt aagcgctaca gccaaacaag tgcaggtcgt cagcaaacag gttctggttt
     3721 aggcttaatg atctgcaaag aattaattaa aaatatgcag ggcgatttat cattagaaag
     3781 tcatccaggc ataggaacaa catttacgat cacaatcccg gtagaaatta gccagcaagt
     3841 ggcgactgtc gaggcaaaag cagaacaacc catcacacta cctgaaaagt tgagcatatt
     3901 aatcgcggat gatcatccga ccaacaggct attactcaaa cgccagctaa atctattagg
     3961 atatgatgtt gatgaagcca ctgatggtgt gcaagcgcta cacaaagtca gtatgcaaca
     4021 ttatgatctg cttattactg acgttaatat gccgaatatg gatggttttg agttgactcg
     4081 caaactccgt gagcaaaatt cttccttacc catctggggg cttacagcca acgcacaggc
     4141 taacgaacgt gaaaaagggt taagttgcgg catgaactta tgtttgttca aaccgttgac
     4201 cctggatgta ctgaaaacac atttaagtca gttacaccag gttgcgcata ttgcacctca
     4261 gtatcgccac cttgatatcg aagccctgaa aaataatacg gcgaatgatc tacaactgat
     4321 gcaggagatt ctcatgactt tccagcatga aacgcataaa gatctacccg ctgcgtttca
     4381 tgcactagaa gctggcgata acagaacttt ccatcagtgt attcatcgca tccacggtgc
     4441 ggctaacatc ctgaatttgc aaaagttgat taatattagc catcagttag aaataacacc
     4501 tgcttcagat gacagtaagc ctgaaattct tcagctgctg aactctgtta aagaacacat
     4561 tgcagagctg gaccaggaga ttgctgtttt ctgtcagaaa aatgactaaa ttgcggctcc
     4621 cacaatgttc taatgtggga gctatttacc agcacatctt acatttatga tgagaactcc
     4681 tggcgaattt gttccccatg ctggtcgagc gttgccgctc ccggcataac atgcgggtcc
     4741 gcgcagccgc tgatttttat cggattaccc ggcatcatta ttcctccggc ttcaatcaac
     4801 atatttctcg cctgagtttg tggcaaatta atggcctcag ccacacttaa taacggcgcg
     4861 acgggcacac caacttcatg tattttggct aaccaaactt cagctgcctg cgtttttaac
     4921 gttcgctcaa tatattgttt aagaatagcc tggttttgta cgcgtaaaat attgctgcta
     4981 aatcggggat cattaaccag ttccgtaagc tccagtgcct ggcataacgc agaaaaaagc
     5041 ttgtcattac cacaacaaat cgtaatcggc ttatcctgag tatcgaaaac atcaaaaggt
     5101 gccatgtagg gatggcgatt tcccaggcgt tgtggtgact tccctgtcgc aatatatgcc
     5161 atcagaccat gctccagaaa actcagcgtg gcatcaaaca tcgctatatc gacatgcgcc
     5221 cctctctggc tcttttcgcg gccataaagt gcactcacta ttccgctgaa taaatagaca
     5281 ccgccgcata gatccgcaag agaggtacca acacgcactg gcggagcatc agggtaccct
     5341 gtttccatca ttatcccact cattgcctga atgatggtat cgtaggcagg agcatctttt
     5401 agcggaccgg tatgtccgaa acctgacgat gaagcatata tgaggcgcgg gttgatttct
     5461 tgtaaccttt cccatgaaaa ccccagtttt tccattgtac ctaggcgaaa attctcagct
     5521 aatacatcag cttgtttgag catatttata aatatacttt tatcgtgatc attctttaaa
     5581 tcaagaacca cactctcttt gccatgatta ataaaactgt aatagagtga ctgtccatcc
     5641 acatagggac caaatgtgcg ggtatcatca ccatgacctg gtggctcaac tttaattacc
     5701 cttgcgccca tattataaag aagttgagtt ccgaaaggtc cattaaggac atgtgtcata
     5761 tcgataacta ataagccttc aaacggccct ttgctttcat tatttgtcat ttgcttatgc
     5821 cttgaaaaaa gccgaaggtg aaaagacact tcggcattta tgtaaaatca ataacatgat
     5881 taattatgaa accagtcgac tgacataaat ccacaaggga gccgtgacga caaagcccag
     5941 tacgctcaca gccaatgacg ctgtaccagt acgggtgtag acattaaacc ggctggcaat
     6001 aatgattccg gagaatgccg gcggtaatgc gcctgccagt accatcatct gcaaatgttc
     6061 gctgttcaaa tgacatgcca taccaacgag aagcagcgcc agtggcatca gaatcaactt
     6121 caggaaggtg ttataagcaa tttcggcact gaactcgaat ttatgtgcag ccagagtcaa
     6181 ccccgcagcg aataccgcta cccctgagtt agccttcgca atcagattaa aggttgggtc
     6241 ccatgctgcc ggaattttta ccccaaccag caccaggatc gttgccagaa caggtgccca
     6301 tactactggc tcctttgccg cagaaattaa tgcgctcaga ttactattct tcttaccatc
     6361 cgctcctgag gaaggattca gcaaatacag accaatagga atagtaattg cgttaacaat
     6421 aatagaaata attgctacca ctaaacctgt tgatacggaa tcaccataaa taggatcgag
     6481 aactgcaaac cccaggaatc caatggtagg tgaacccgca attaatgcac atacagctgc
     6541 ttctgcatgg gtacgtttaa aaaatttgta gcaaccaaac caggagaaaa agaaacatcc
     6601 gacaataacc acaagtgata caagggtcag acgagtgtcc gcaaaaatca tttccctgtt
     6661 tgcccgggta atagaaacaa atagagccgc aggaagagcg tagtttaata ccagtttatt
     6721 aaatgcgcgg gcttgatctt ctgaaaaagt ttctcgtctg ccgctaaaat aacccaacag
     6781 catgattacg ataataggca ataaatcgcc aataaaaaat gttagcataa tagtatttcc
     6841 gataagggtc ccctcaccat cgcataatga gttggcaaga ggagacgatg tcagtattta
     6901 attaccagcg acttgttttg ggttaagttt ggtaatatgg ccactttcag ttcctgctgc
     6961 agggtcgata accacattaa taatggtcgg tttgcgcgac tgaataccgg tggttaaagc
     7021 atgacgaagt tcatctgtcg tggtgacgtt atagccaacg ccacgaaacg catccattaa
     7081 tttgtcatac cttgcatggt gcaacagatc cgttggtgat ggtgcaccag cgccactgag
     7141 atcaacaccg tctcctctgt agatgccgcc attattaaaa ataacgatcg tcaccggcag
     7201 gttatatcga caaatcgttt caatttccat cccactgaaa ccaaaagcac tatcaccttc
     7261 aatggcgaca accggagaac cagaagtcac gctagcaccg atggcatagc ccataccgat
     7321 gcccatgaca ccccaggtac cacaatccag acgacgacgt ggtttataca tatcaataat
     7381 atttcgtgca ttatccaggg tatttgcacc ttcattaact aaataaatat cctggttctc
     7441 gcgcaatacg tcgcgcacag cacttaatgc attaaagtaa tttaatggtt gagtatctgt
     7501 acttaatttt tcatgcattt tttgtgcatt ttgctgcttg tggatattta aaatatcgcg
     7561 ccataccagt ggagtcgtaa atgtgttttg tttcagttct gccagcatac cttgcatact
     7621 ggatgcaata tcaccaacga ctggcacagc aatggggcgg ttgctgtcaa tttcctgcgg
     7681 ttcaatatcc agttgaataa actgtgtatc tgccgcccat ccttttttac cgtgtgccaa
     7741 taaccaattc agtcgtgcac caacaagcat gacaacgtca gcatttgcca gggcaaacga
     7801 acgcgcagct gccgcagaaa gtggatgcgt atcttcaagg atccctttcg ccatagacat
     7861 tggcaggaat ggaatctgag cactttcaat aaattcacga agctgttcat cagcttgtga
     7921 atacgccgcg cctttgccaa ggataattaa tggccgttca gcttttgcta aaagcgaaat
     7981 tgcgctagtg actgacttcg ggcatggcaa taatgctggc gacggatttt caactttaac
     8041 aatcgtggtt aacgcttcgt ctttttccat cgtcgcggcc aggacatttg ctggcaaatc
     8101 aagataaact ccgccagggc gacccgatac agagacccgg atagcgcgtg ccaatgcaat
     8161 gccaagatcc tgcggctgat taacgcgaaa tgctgctttg gcatacggtt ttgccgcatt
     8221 catttggtcc agctcttcat aatcaccttg ctgtaggtcg acgatcgcgc ggtcgctgga
     8281 gccgctaatc atgataatcg gaaaaccatt taccgttgcg ttggccaatg cggtcaaacc
     8341 attgaggaat cctggcgcag aaactgtcag gcagatcccc ggtttttggg taagaaaacc
     8401 gcttgccgca gcggcatagc ctgccgactg ctcatgacga aaaccaatat aacgaatgcc
     8461 ttccgcctgg gcatggcgtg ccatatccgt cacaggaata cctacaacac cataaatagt
     8521 gtcaatatta ttctgtttta atgcttcaac gatgatatgc ataccatctg tcatttgaag
     8581 ttgatctgac atgattaata acctcgaaat ttttataaat tgggagccag aagcccccgt
     8641 taatatcaga tggcgtggtt ttgcttcatt gcagcaattt catcgtcgct ataacccagc
     8701 tcctgcaata cagcagcggt atgttcacct aatagcggcg cagctttaat atccggcgta
     8761 aaggcagaga atttcattgg acaaccaacg gtcagatatt ttccacgcaa cggttgttcc
     8821 acttcaacaa cactgccact ttggcgcaaa gagggatcaa gtgaaatttc tttcatactt
     8881 aaaaccggtg cacaaggaat atcaaactga gtcaaatagg ccaccgcttc atgtttatca
     8941 atagtgacag tgtatttttc gatttcagca aaaatatcga aaatatgtgg ctgtcgggca
     9001 tgggctgtac tgtatgccgg atcggtaatc cattctggtt tgccgatggc tttacaggtg
     9061 ttttcccagt tttgctcctg aatagtgaaa taaatatagg cgttaggatc ggtttcccag
     9121 cctttacatt tcaggatcca gccaggctga ccgccaccac ctgcattacc accgcgggga
     9181 actgcatcac caaatgtacc attcggatac tgcgggtatt cttccagata acccaattta
     9241 tcgagacgct gctggtcacg taatttcacg cggcaaaggt tcaatacggc atcctgcatt
     9301 gacatggtga ctcgttgccc acgccccgtt ttttcgcgat gcagcaaagc agcaagtaaa
     9361 ccgatcagca aatgcattcc ggtgttgctg tcacccaacg ctgcagcgct taccagcggc
     9421 ggaccatccc aaaaacccgt agtggatgcc gcgccacccg ctgcctgagc aacgttttca
     9481 taggctttta cattcacata aggcgaacac tcatcaaacc ctttgatcga accaaaaatc
     9541 agacgtggat tgatttcttg aatatgctcc caggtgaagc ccatgtgatc aatggcccct
     9601 ggatgaaagt tctcgactaa gatatcagct tcgcggatca gcttttccat tacctctttg
     9661 ccttccgctg ttttggtatt taactcaata gaacgtttgt tactgttaag catggtgaag
     9721 taaagcgcat cgatatcagg aatatctcgc agttggtgac gcgttacgtc accaacgccg
     9781 ggacgttcaa ttttaataac gtcagcgcca aaccaggcca gcatttgagt acaagatggg
     9841 ccagattgca caccggtgaa atcgagaact ttaattcctt gaagtggagt tgacatagca
     9901 atacctatta gttaattaaa tatgtgttaa tttagcgttt atttaaggca taacgccctc
     9961 tataaataat ctgcaaaata tcttcgtagc ggcactcgat atttttaaat tcctgtatca
    10021 agtactgaaa attattttat cgccacttcc aattcctttc tgcatggtaa gtagtgagga
    10081 cgaaaagaag aatatgataa atattaattc gtggtaatca gagagagtcg cgacatttgt
    10141 gatgttattc ttacaactgt aagaagcgcc ggtgatttac aagtaagagt atgattacgc
    10201 tttttaaagc gtaccataaa gcataacata ataaatgatt tgtgatgaca cgggattatt
    10261 gcggtttact ttgaggaaaa taaaatgcgt gtttatagaa ccgcgagcgc caaataattt
    10321 tatgaccaca acctgtcgta ttgtggtcat aaaaaattgt cggctggagg gagttaaatc
    10381 ctttacctca atatactgcc tgtcagcatt agttcacaaa cagtgcttcg ctatcgacga
    10441 tgattccatc ttccgcacct ttaagcgcca ggtttgcctc gtagtattgc tttttatcaa
    10501 gcagtttctg cgcatcagca agtgcattac gagtctgttt aagcggcatc agatattggt
    10561 tttccattac gccaacaccg gccaggcgaa gctcttccat tgcgcccttt ttatcccctt
    10621 tcgccatttt ttcattggca atttttatcg cggcttcttt ttcgggtgtt gctacataac
    10681 tctcagaaat tccgacagat gcattaatga caatatattg gtcatcattg agattggtct
    10741 ttttacctgg cttagcgaac tttgcccatt cggtactgtc atctgacagc aaagcggaag
    10801 cttcattggt cagttctttt gccttttccg gatcgccatg aaacagagcc aggcgcgcta
    10861 cctgcacgtc acgcatcgca tataaacctt gttcagagat acgctctgct gctgccgttt
    10921 tctgggtttg ctgagtttgc tgttgtgccg ctaccggtgc attatcagca gcccatacag
    10981 ttgaagatgc cagaattgct gtgaccatcg tagccataat taaacgtttc atacttacct
    11041 cattctttaa cagttttaga aaacgccgcc atcatattgg gtgagcatga aatgagaaat
    11101 cagagagcaa cggtcatttt gaaagggtta tcttacaatt gtagttatta tcgcaaagcg
    11161 gcatttagtc ttacagttgt aggaatatgc ctcctgtcgc agagaactat tcttatatac
    11221 cacaaagaga tgtcatttat tatattgatg taaatattga ttaattatat gtaaatacag
    11281 ccataacatc gtatggttct cagtgaacca ttttcaggat aactttctat gaaagtaaac
    11341 ttaatacttt tcagcttatt tttattggtc tctattatgg catgcaatgt ttttgcattt
    11401 tccatttctg gtggtgtaag tgagaggagc tataaagaga ctgaaaaaac atcagcgatg
    11461 acgaccacac actctacaaa acttcagcca tcacaggcga ttttgtttaa gatgagagaa
    11521 gatgtgccac cattaaacct cacagaagaa atcacgccta cttatccgac aaaggcgaat
    11581 tatcttattc atcctgtgcg atagcgtctg tcacatggca ggtgaacaat catgtctgat
    11641 gctgatgtga acacctgccc aaatgagcca tcctgaccca ctatcgcctg ccatacccca
    11701 ccataaagcg aatcatcaag ccacaagccg gggcaagaaa cataaatatc cccgtcacca
    11761 gttcattacc gccagttaac cacatggtga agtaataaat gagtgcagcg aggatcacca
    11821 tgccgaagaa agaagagacc gccgatacca cgttcagtac ctgaccgatg tagccgctta
    11881 cgcagacaat acacagggcg agaaacatcc ataaataaat catagctcaa cctcctgccg
    11941 cgctaaaaaa cctgtaactt cagtcacttt aatagtaaga caatccttaa cctctggcaa
    12001 tttttgctaa gcatcaatga aaacagcacg ttaaatgcaa cgctattgcg tgcggaaaat
    12061 tttttacttt gcacgattaa taatcagtag ctgaaagcag tcagccagtg ataaagtaga
    12121 gccaccgcat tcatgctcct cgtggttatg tcctgactag tctttacact ctttacagga
    12181 accattgtcg tacatgatgg cccaaccaat tgagtgtacc gctgtcgaca tggaatcttc
    12241 tgcgatacaa caattcgtat ctacagaagg taactatgtt tccacaatgc aaattttccc
    12301 gcgagtttct acatcctcgc tactggctca catggtttgg gcttggtgta ctctggcttt
    12361 gggtacagct tccttatcct gttctctgct ttctcggcac gcgtattggc gcaatggcgc
    12421 gaccattcct gaaacgtcgt gaatctatcg cccgtaaaaa cctggaactt tgtttcccgc
    12481 agcattctgc ggaagaacgc gagaagatga ttgccgaaaa ctttcgttca ctcggcatgg
    12541 cgctggtaga aaccggcatg gcatggttct ggcccgacag tcgcgtacgt aaatggtttg
    12601 atgttgaagg gttggataac cttaaacgcg cacaaatgca aaatcgcggc gtaatggttg
    12661 tcggcgtcca ttttatgtcg ctggaactgg gcggccgcgt gatgggactg tgccaaccaa
    12721 tgatggctac ctatcgtcca cataataatc agctgatgga atgggtgcag acccgtgggc
    12781 gcatgcgctc taacaaagcg atgatcggca gaaataatct gcgcggcatt gtcggtgcac
    12841 tgaagaaagg tgaagcggta tggtttgctc ccgatcagga ttatggtcgt aaaggcagct
    12901 ccttcgcgcc gttctttgcg gtggaaaatg tcgccacaac caatggcacc tatgttctct
    12961 cccgtctctc tggcgcagcc atgttgaccg taacgatggt aagaaaagcg gattacagcg
    13021 gatatcgttt gttcatcacc ccagagatgg aaggctaccc gacagatgaa aatcaagccg
    13081 ctgcttatat gaacaagatt atcgagaaag agatcatgcg cgcaccggag cagtacctct
    13141 ggatccaccg tcgctttaaa acgcgcccgg tgggagaatc gtcgttgtac atttaacaaa
    13201 ctgctcttac tctttccctc ctcaaatcgg ccataataac ctcatgattt ttatagcttt
    13261 tttcttaagt tttttccttc gctgaatatc taattattac cgtcagaaat taaactgtcg
    13321 ccgatatgag acacttgtaa gttactaaaa gtaaataaga aaatgcctct tgtctcgttt
    13381 cagttttagg cgtacattag cgccgtctcg agcagtttag ctcagaattc tggggtgatg
    13441 cccacggtaa aatacagggc ttttaaggtc gctttgcccg gtgtcaactc actgtatcag
    13501 gtgtaatgaa gtcattcagg cgtaacagta attacgcgga gagatgtaaa gtgaaatatt
    13561 tctttatggg catttctttt atggtcatcg tttgggccgg tacttttgcc ctgatgatct
    13621 aaaagcaaac tgtgcaatga aaaacaggag ccatcaggct cctgtttgca tttctgacga
    13681 cggtgtttat tccgcgtttt cgtgaatatg tttgctgctg acgggtagta aaccgtcggc
    13741 acggaacatc gctttaatcc ctctgatggc ctgacgaata cggtcgtggt tttcaatcag
    13801 tgcaaagcga acatgcgtat caccatagtc gccaaagcca atccccggcg agacacaaac
    13861 cttcgcttcg ttaaggagct tcttggcaaa ttccagcgat cccatggccg catatggttc
    13921 cgggattttc gcccagacat acatcgaagc cttcggcatt tcgaccatcc agcccgcttc
    13981 atgcagccct ttaaccaata catcacgacg gcgtttgtac tgttcggcaa tgtcgcgcac
    14041 gcactgttga tcgccctcga gtgccgcaat cgccgccacc tgcaacggcg taaaagtgcc
    14101 gtaatcgtga tagcttttga ttcgcgccag ggcactgacc agcgttttgt tgccgaccat
    14161 aaagccgata cgccagcccg ccatgttgta gcttttcgac agcgtaaaga actcgaccgc
    14221 cacatcgcgt gcacccggca cctgcataat tgacggcgct ttccagccat cgtagacgat
    14281 atcggcatag gccaggtcat ggaccaccag cacatcgtag catttcgcca gcgccacaac
    14341 cttttcgaag aactccagtt ccacgcattg cgcggttggg ttcgacggga agccgaggat
    14401 catcatcttc ggtttcggat aactttcacg aatggcgcgc tccagttcgt tgaagaaatc
    14461 gacaccttcc accagcggca ctgagcgtac ctgcgccccg gcaatcaccg cgccataaat
    14521 atggatcggg taacttggat tcggcaccag caccgtgtca ccatgatcca gcgtcgccag
    14581 catcagatgc gccaggccct ctttcgaacc aatagtgacg atggcttctg attccgggtc
    14641 gatttcaacg tcgtagcgat cctgatacca gcgggaaatg gcgcgacgta accgcggaat
    14701 gccgcgtgaa gtggagtaac catgcgtgtc cgggcgctgg gccacagtac ataatttttc
    14761 gacgatatgc ggcggagtcg caccgtccgg gttacccatg ctgaaatcga taatatcttc
    14821 gccgcgccga cgcgcagcca ttttcagttc agcggtgatg ttaaaaacgt agggcgggag
    14881 acgatcaatg cgcgtaaagc gacgttcagg gcgagtgtca gccataattt cctcagatta
    14941 acgttagcgc ccggaccgtc cgagcgacgc tgccacaatg gtggctgttt tgaaaatagc
    15001 ctgattaatt tctgtctgtc cagcgtttta gaacattttt atcaatggat ttaaatgagg
    15061 aactccactt caaaaacagg aaatttttct acgctaacaa gaaaatcacc accaatttta
    15121 ttaacattat aatttcaata aatttaccag ccagaagtgc attatcaaga gatacattgc
    15181 taatacacca tttatctttt aatccccttt taaatttatg gtttttcctt aaaaacggcc
    15241 tgctggtcat cagccttcag gttacctatc atagaggttt aatccttatt cagagtcacc
    15301 cgtgcacgaa atattcaaca tgctgctggc ggtcttcgat cgggcagcgt taatgcttat
    15361 ctgcctgttc tttctcatcc gtatccgcct gtttcgcgaa ctgttgcaca agtcggcgca
    15421 ctccccaaaa gaattgctcg ccgttaccgc cattttctcg ctgttcgccc tgttcagcac
    15481 ctggtccggc gttcccgtag aaggctcgct ggtgaacgta cgtattatcg cggtgatgtc
    15541 cggcgggatt ctgtttggcc cgtgggtagg catcattacc ggcgtgattg cgggtattca
    15601 ccggtattta attgatatcg gcggcgtgac ggcgatcccc tgctttatca ccagcattct
    15661 ggcgggttgt atatcaggct ggataaacct gaaaatcccc aaagcacagc gctggcgctg
    15721 gatatcgcca acaaaacgct accgctgttt cgccatgtca atagcgagtc attacgcaag
    15781 gtctgcgaaa ttatccgcga tgacattcac gccgatgcgg tggcgattac taataccgat
    15841 catgtgctgg cctatgttgg cgtgggtgaa cataactatc agaatggcga tgacttcatt
    15901 agcccgacta cccgtcaggc gatgaattac ggaaaaatca tcattaaaaa caatgatgaa
    15961 gcccaccgca caccagagat tcattccatg ctggtgatcc cattgtggga gaaaggggtc
    16021 gtgaccggaa cgctgaaaat ttactactgc cacgcgcatc agatcacctc gtcattacag
    16081 gaaatggcgg tcggtctgtc gcaaatcatc tccacgcaac tggaggtttc acgcgccgag
    16141 cagctacgtg aaatggcaaa taaggcagag attcgcgccc tgcaaagcaa aattaatccc
    16201 cattttctgt ttaacgctct gaacgctatt tcatcgtcaa tccgtctgaa tccggatacc
    16261 gctcgccagt tgatctttaa tctgtcgcgt tatctgcgct ataacattga attaaaagac
    16321 gatgagcaaa tcgatatcaa aaaagagctg tatcaaatta aagactatat tgccattgag
    16381 caggcccgct ttggcgacaa gctgacggtt atctatgata ttgatgaaga ggtgaattgc
    16441 tgcattccca gcctgctgat ccagccgttg gtggagaacg ccattgtcca cggtattcag
    16501 ccttgcaaag gtaaaggcgt tgtcaccatc agcgttgcag agtgcggaaa tcgggtacgc
    16561 attgcggtgc gagataccgg gcacggcatc gttccaaagg tgattgagcg ggtcgaagcg
    16621 aatgaaatgc cgggcaataa aattggcctg ctgaatgtcc atcatcgcgt gaagttattg
    16681 tatggcgagg ggctgcatat ccgccgcctg gagccgggga cggaaattgc gttttacatt
    16741 cctaaccaac gcaccccagt cgcctcacag gctacgttat tgctttgagc cggagtgata
    16801 ttgtgaaagt catcattgtt gaagacgaat tcctggcaca acaggaactg agctggctaa
    16861 ttaaagagca cagccagatg gagattgtcg gcacctttga cgacggtctg gacgtgttga
    16921 agtttttgca gcataaccgc gtcgacgcca tttttctgga tatcaatatt ccgtcgctgg
    16981 atggcgtgtt gctggcgcaa aacatcagcc agttcgccca taaaccgttt attgtgttca
    17041 tcaccgcgtg gaaagaacat gcggtagaag cgtttgaact ggaggcgttt gactacattc
    17101 tcaaaccgta tcaggagtca cgtattaccg ggatgctgca aaaactggaa gcggcctggc
    17161 aacaacagca gaccagcagt acgcctgccg cgacggtaac gcgtgagaat gacaccatta
    17221 atctggtgaa agatgagcga ataatcgtca cgccaattaa cgatatctat tacgccgaag
    17281 cgcacgagaa aatgaccttt gtctatacgc ggcgtgaatc ctacgtaatg ccgatgaaca
    17341 ttaccgaatt ttgcagcaaa ctgccgccgt cgcatttttt ccgctgccat cgctcatttt
    17401 gtgtcaatct gaacaaaata cgcgaaatcg aaccgtggtt taataacacc tacattctgc
    17461 gactgaaaga tctggatttt gaagtgccgg tcagccgcag caaagtgaaa gaatttcgcc
    17521 agttaatgca tctttaatcg ggaactttca tgaaagcacc aggcttgccc gccgatcaac
    17581 aatttttcgc cgatctgttc agcggcctag tgcttaaccc gcaactactg gggcgcgtct
    17641 ggtttgccag ccagcctgcc tcgtta
//
LOCUS       DAZSNM010000074        17642 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZSNM010000074 DAZSNM010000000
VERSION     DAZSNM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17642
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.074"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..675)
                     /gene="chbR"
                     /locus_tag="ACHW42_002051"
                     /pseudo
     CDS             complement(1..675)
                     /gene="chbR"
                     /locus_tag="ACHW42_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(683..1033)
                     /gene="chbA"
                     /locus_tag="ACHW42_002052"
     CDS             complement(683..1033)
                     /gene="chbA"
                     /locus_tag="ACHW42_002052"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR8943878.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1092..2440)
                     /gene="chbC"
                     /locus_tag="ACHW42_002053"
                     /pseudo
     CDS             complement(1092..2440)
                     /gene="chbC"
                     /locus_tag="ACHW42_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2525..2845)
                     /gene="chbB"
                     /locus_tag="ACHW42_002054"
     CDS             complement(2525..2845)
                     /gene="chbB"
                     /locus_tag="ACHW42_002054"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR8943879.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3144..3482)
                     /gene="osmE"
                     /locus_tag="ACHW42_002055"
     CDS             complement(3144..3482)
                     /gene="osmE"
                     /locus_tag="ACHW42_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR8943880.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3684..4511
                     /gene="nadE"
                     /locus_tag="ACHW42_002056"
     CDS             3684..4511
                     /gene="nadE"
                     /locus_tag="ACHW42_002056"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR8943881.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4741..5628
                     /gene="cho"
                     /locus_tag="ACHW42_002057"
     CDS             4741..5628
                     /gene="cho"
                     /locus_tag="ACHW42_002057"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR8943882.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5588..6163)
                     /gene="ves"
                     /locus_tag="ACHW42_002058"
     CDS             complement(5588..6163)
                     /gene="ves"
                     /locus_tag="ACHW42_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR8943883.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6366..6851)
                     /gene="spy"
                     /locus_tag="ACHW42_002059"
     CDS             complement(6366..6851)
                     /gene="spy"
                     /locus_tag="ACHW42_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR8943884.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7181..8149)
                     /gene="astE"
                     /locus_tag="ACHW42_002060"
     CDS             complement(7181..8149)
                     /gene="astE"
                     /locus_tag="ACHW42_002060"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR8943885.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8142..9475)
                     /gene="astB"
                     /locus_tag="ACHW42_002061"
                     /pseudo
     CDS             complement(8142..9475)
                     /gene="astB"
                     /locus_tag="ACHW42_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9472..10950)
                     /gene="astD"
                     /locus_tag="ACHW42_002062"
     CDS             complement(9472..10950)
                     /gene="astD"
                     /locus_tag="ACHW42_002062"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFR8943886.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10947..11981)
                     /gene="astA"
                     /locus_tag="ACHW42_002063"
     CDS             complement(10947..11981)
                     /gene="astA"
                     /locus_tag="ACHW42_002063"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR8943887.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11978..13198)
                     /gene="astC"
                     /locus_tag="ACHW42_002064"
                     /pseudo
     CDS             complement(11978..13198)
                     /gene="astC"
                     /locus_tag="ACHW42_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13635..14441
                     /gene="xthA"
                     /locus_tag="ACHW42_002065"
     CDS             13635..14441
                     /gene="xthA"
                     /locus_tag="ACHW42_002065"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR8943888.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14608..15321
                     /locus_tag="ACHW42_002066"
     CDS             14608..15321
                     /locus_tag="ACHW42_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR8943889.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLLYARHK
                     HVDLSASRRSPLTHPKNEG"
     gene            15326..16003
                     /gene="ydjY"
                     /locus_tag="ACHW42_002067"
     CDS             15326..16003
                     /gene="ydjY"
                     /locus_tag="ACHW42_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR8943890.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16018..16725
                     /locus_tag="ACHW42_002068"
     CDS             16018..16725
                     /locus_tag="ACHW42_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR8943891.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16725..17273
                     /locus_tag="ACHW42_002069"
     CDS             16725..17273
                     /locus_tag="ACHW42_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR8943892.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17283..17609
                     /locus_tag="ACHW42_002070"
                     /pseudo
     CDS             17283..17609
                     /locus_tag="ACHW42_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4361 a         4688 c         4308 g         4285 t
ORIGIN      
        1 gatctcatta ataatctgca ttggtgtttt gccataatat cgttgagtcg ctcgcgtcaa
       61 atattcctgt gatttggctg acaacgtcac catattctcc agcgccgatt cactaaactg
      121 ctctttatca tgcatctttt ctaccgtact tttcagccac tacggtacat catcaatcac
      181 ctgttcttcg cggtaatgac gtaaacggtt aatgacataa aaagtaacca tctcaacaaa
      241 ctcttccagc cctgtttcgc ggaaatttaa tgaagaaatc actgtttcca cataggtgag
      301 aaaagcgtta ttggtccggt atacctgcga agcaacaaag caataaggca acaacggcag
      361 gtaatgctgc tcaaaaaagc gtttactgat cccaacgttc aatatgcgcg tggcaccaaa
      421 ctcataaaaa ctttgatggt gcgaacctaa cggaataaaa acaaaatcgc cccgttccag
      481 taacacgcgc ttaccgttaa tctcctggaa ataacgcccg gttaatacca gagtaaattc
      541 ataatagtcg tgctgatgca gtccgctgat actctcagtt ttgttataga taaacacatg
      601 gaagtttttg ccattaaaca actgctgttc tcgggcagtg gcaatttccg gcgcgttaat
      661 cactggctgc atcatcgact ccttatgcct tcagtttttc atgaagctca attaattcag
      721 taatcagttc acgcgcaagc atggacgtca ttaaatgatc ctgagcgtgg accagcacca
      781 gactcacttt catcttacct tcgcccgcat cgccttcaat cagtttcgtc tgtaccagat
      841 gcgcttcatt caatgccatc cgtgactgat ccatcatggc tttcgctgcg gcaaaatcgc
      901 cctgcttcgc ctgtttcaat gcggcatacg ccaggctacg cgcttgtcca gagttgatga
      961 taagccccat caccacttct tccagctctt cagcttctgt ttgcgtatca ggaatattat
     1021 cgagatccat catacatcgt tcctctctct tcttaccggc aagattaccc gtaccggcat
     1081 cgattaaaat ttcaggggtt agcgatatct tcttcgctct cttctttatc aatcgcattt
     1141 tgtgctttgt tagccaccac aacaaagggc agataaatta acgttgcgat gccaaggttg
     1201 aagagtgcga ccagcaatgc ggcgacgcta ccgttggtgt taaagaaggc tcccagaccg
     1261 gttggcatgg tccacggtgc aatattggtc accggaggaa tgatgcccat gtagtacgct
     1321 gcgagggtga ttgccgccag aatcggttgt accagtacaa acgggataaa catcaccggg
     1381 ttcatgataa ttggcagacc aaacagaatc ggttcgttaa tctggaagat gccggacggc
     1441 agcgccagtt ttgccacctg acgataatca gcacggcgag aggcgataaa gatagccagg
     1501 atcaggccta aagtcgcacc actgccccca aggaaaataa aggagtccag catcggcttg
     1561 gcccagatat ggaaggtctt accggctgcc agcgccgctt cgacggaacc atattgctga
     1621 taggtcgcga tattttccag cgcccacggc gtcataatgc cgttgtccag tgcggtcagc
     1681 gccagcgcgc catgaatacc gaagaaccag agcagtggaa caaagatcac ataggcccag
     1741 cccaccacgc tacccaacga tgccagtggg gttgagatgg tatccataat gatctgatgg
     1801 aagttggtgc cccaggtatt caacgcccag gcaataatcc ccatcacgga aagaataata
     1861 aagccgggaa ttaatgccga gaaagaacgc gatactgaag caggtacgct gtcgggcagt
     1921 ttaatgaccc aattgcggcg gacaataaag gtaaacattt ctgccaccac caggccaata
     1981 ataatcccgg agatgatatt cgccccacct aaccagtttg cgccaaccgc ataggcctca
     2041 ccgacactat atggggtgac ggtcataaat gctgcaacgg ataacaaccc agccgccagc
     2101 gcatcgactt tacgctcttc tgccagcgcc atgccaataa agaacggtgc cattaaagac
     2161 attattccta atgttccgtt atatacgttg ccgccaatac ctttcagacc attaagtgtt
     2221 tcaatggttg aggcgtcgag gcgaatacct aaggaataaa aaacgacccc tccccaaagc
     2281 ttagaaaaac gttgttaatt aatacaaaca tggcccccgc aagggttaac ggcattaagc
     2341 gaataaagcc atttttgatt gcattaacgt gtggctgctt tcctatttta actgcaaaag
     2401 ggaggagtac cttttcaagc gatgcaataa cattactcat agaaaaatac ccttaaaaac
     2461 cgcaatttaa atattacggt attgatttat gaaataactc tttgacggga aaatttaaaa
     2521 taaattaatt tgctgcggct tttttaatcg ctgcaaccgc agccttaagc acgcctaaac
     2581 catcgacttt gccataaagc agcgagtcaa ttacttcaac cggtttgttg ggtaacaaac
     2641 gctggatttc gggcaacata taagcaatct gcggccctaa taacacgaca tcggcattct
     2701 gacctttttc accagccagt gtttccggaa atgcttcaat aatgaccgga acttcatatt
     2761 tttctgcctg tgcgcgcatt tttgatacca gtaaagagat agacatgccc gcagaacaaa
     2821 acagataaat gtgtttcttt tccataaaac tgccctcatc gacgattatc tgtcagccag
     2881 acactccgca agccttgacc tgcttccatg ctctgggtaa cttgcgaaac caacatgact
     2941 ttttttgtgg ctgaaaggag tatacggtat agacctgagc aatagtattt ctttgacctt
     3001 ataaattgtt tctccttgac ctgtggttat gaccctcttc acatttcggg caaataattc
     3061 gcgaagataa ttaagaactc aggccaaaaa aaaccggcgc aatggccggt ttccgttgtt
     3121 actcaagctt tcagacgaat tgattacttc gcagcctgtg gatcagtgtc gtattcagca
     3181 caggtctgat aaccggagtt gatgacatgt ccggtatcat ctaacgcgac aaagtaggtt
     3241 tctgctttac catcacgttg acccaggatg taggtctggc aagtaccgcg agcatggatc
     3301 atgctcactt cagacgaagg tttacccgca atttgtgcaa cctgcgcccg gctcatgcct
     3361 tttttgacgt ctttcaccac aggctgtaca aactggtctt tggtacgatc ataagctgta
     3421 caacccgcca gcatggttaa taccgccgct gcactcagaa ttcctgccat attcttgttc
     3481 atattccgtc ctcttgttta tcagcgtgtt agataagcct ggaatacatt gggcgctttt
     3541 tcaagcccgt gaacgaaacg gctccgcttt cagaggattc ctgtatgacg ttttaaccac
     3601 cattcagccc gctgtcgctt gtcgtttcag tagcaacggg ttagctttaa ggaagttttg
     3661 tcttttctgt ctggaggggt tcaatgacat tgcaacaaca aataataaag gcgctgggcg
     3721 caaaaccgca gattaatgct gaagaggaaa ttcgtcgtag tgtcgatttt ctgaaaagct
     3781 acctgcaaac ttatccgttc attaaatcac tggtgctcgg gatcagcggc ggtcaggact
     3841 ccacgcttgc cggaaagctg tgccagatgg cgattaatga gctgcgccag gaaactcgca
     3901 acgaatcact gcaatttatt gccgtacgcc tgccctatgg tgttcaggct gacgaacaag
     3961 attgccagga tgccattgcc tttattcaac cggatcgcgt attaaccgtt aatatcaagg
     4021 gcgcggtatt ggctagcgag caggcattgc gggaagcagg cattgaactg agcgattttg
     4081 tccgtggcaa tgaaaaagcg cgtgagcgga tgaaagcaca atatagcatt gcgggtatga
     4141 ccagcggtgt cgtggtgggc accgatcatg cagcagaagc cattaccgga ttcttcacta
     4201 aatatggtga cggcggtacg gatattaatc cgctgtatcg tctcaacaaa cgccagggta
     4261 aacagttact gacggcatta ggttgcccgg aacaccttta taagaaagcc ccaactgccg
     4321 atcttgaaga tgatcgccct tctctgccag atgaagtggc actcggcgtg acctatgaca
     4381 atatcgacga ctatctggaa gggaaaaacc tacctgaaca ggtcgccaga acaatagaga
     4441 actggtatct gaaaaccgaa cataaacgcc gtccgccaat taacgttttc gatgatttct
     4501 ggaaaaagta ataatttgca cctattggat tgtgcgaaaa agagtaattt gttcacgccg
     4561 gatgcggcgt gaacgcctta ttctacctac aaaactatgc aaattcaata cattgcagga
     4621 gtcgaatagg cctgacaggc gtagcacgtc agacggtgta acctttgtca tcaacccgcc
     4681 tcttttttaa tcgcctcccg cctgttacac tggatagata accagcattc ggagtcaaca
     4741 gtggtacggc gtttaacttc tccgcggctc gaatttgaag ctgcggcaat ttatgaatat
     4801 cccgaacatt tacattcatt ccttaatgac ttacccaccc gaccaggggt gtatctgttt
     4861 catggcgaaa gtgacaccat gccgctctat atcggcaaaa gcgttaatat ccgcagtcgg
     4921 gtgctttctc atttacgcac cccggatgaa gccgccatgc tacggcaatc ccggcgaatt
     4981 agctggatct gcaccgcagg cgaaatcggc gctctgctcc ttgaagcgcg attaatcaaa
     5041 gaacaacagc cgctgtttaa taaacggttg cgccgcaatc gccagctctg tgccctgcaa
     5101 ttaaatgaaa agcgcgtcga tgtggtgtat gccaaagagg tggatttttc acgagccccc
     5161 aacctgtttg gcctgtttgc caataggcgc gcagctttgc aagcattgca gagcatcgct
     5221 gatgaacaaa aactttgtta tggcctgttg ggactggaac cgttaagtcg cggtcgtgca
     5281 tgttttcgtt cagcgctaaa acgttgcgcc ggagcatgct gcggtaaaga gagccatgag
     5341 gaacatgcgc tacgcttgcg ccagtctctg gagcgtttgc gagtggtgtg ttggccttgg
     5401 caaggtgcgg tggcactgaa agaacagcac ccggaaatga ctcaatatca tattattcaa
     5461 aactggctgt ggctgggggc ggttaattcg ctggaagaag cgacaacgtt aattcggaca
     5521 cccgccgggt ttgatcacga cggttataaa attctttgta agccgctgct ttccggtaac
     5581 tatgaaatta ctgaacttga tccggcgaat gaccagcgag ccagttgatt tcactgaaca
     5641 acagtttacc ttgcggttgt aaaagccgca gggtgtgcct gccatcgaac cagcacacgc
     5701 cctggtcggt agtgagcagt ttgtcgccca gttgccacgc cccgttaatc acaaacacca
     5761 ccccgccgcg tgagccaaag gtagtaaacg tacgttcggc aattctgact ttcgccttac
     5821 agacatccag gcgcgtcata atattgaaat ccatcgacat ctgccctgcc gtcagtttcg
     5881 cttttaccac ctggtccgcc gtaaaggcaa aaggctgtaa cggttttaag gtatggttaa
     5941 agcggtctgc gctttcaagg agcatctcgc cgccttccag caacgtcact atcctttcca
     6001 tgccgggaaa caaagaaaac tccccattcg ccgcgatgga tgcaatgctg gcacgccagt
     6061 aaaaatcacg ttttgccggt gggaaagtgc aaatttcgcg cgtttcaccg gcagcgtttc
     6121 gccacagatt caccgacatt ttacgcatat caaagtattc catactcgct cccggcccgt
     6181 tcttttcatt gttatcgttc aggtaaccgt ggatggttat cggcaactcg ggtgatttac
     6241 gttagtggtg atcaggcttt ttttacaact gtttgagaag agaaaagaaa accgccgatc
     6301 ctgtccaccg cattactgca aggtagtgga caagaccggc ggtcttaagt tttttggctg
     6361 aaagattatt cagcagttgc aggcatttta ccttttgccg ctggacgttc tgtcagacgc
     6421 ttctcaaaat tagcattaaa ttgctttttc tgttccggcg tcaggatgtt gtaaattttg
     6481 ttctgggttt ccatgtgcgc cagcatgtta gctttgcgct gttcttccat ttttgcgatc
     6541 tgcgcttcag cttttgcttt atcgaaggta tcgctggcaa tgatgtcatg cattgcgcgg
     6601 cgttcttcca gcggcggacg tttcatctgg tcacgctggc ctttcatgat ttcgtggatc
     6661 tgctgtttct gcgcgtcggt caggttcagg tctttgaaca tcatgtcctg atgcggaccg
     6721 aacttgcctt tgtggtgcat catcggcttc gcgtcagccg gtgctgcggt agtggtgtct
     6781 gcggcatggg ccaggttagc cgcgccaaga gccagggtag aggcaacaaa cagtgcagtt
     6841 aatttacgca tattctatat ccttcctttc agttatttat tacggctttc ttaagtagcg
     6901 tgccgtgttg acgagattaa ctttactgac tttagcgtca attaatcaga gcaacggtaa
     6961 aacaatgaaa gtgtaaaaaa cacttttgcg ccaattatgg agaaaaaaag aaaatttgat
     7021 ggagagtgat gagagaatat tacaacacga tgattttgca gagattatga agaactatac
     7081 cggatgactg gtgataaata aagcaaataa ccaggattaa tctgtattaa tttataagaa
     7141 agcaacttaa tacccgcaga atgatttctg cgggtaagta ttagcttatt ttttcgagca
     7201 ttaatcccgc gcgtaatccc aacgctacca acggattagg gaataacaca tactctacat
     7261 catgggttac ggtaaaacgt tcctctccgt cctgcgccag caatgttcct ttctcaaacg
     7321 gcataaaatt cagcgtgtca cttgccatat gcatttcgaa ggacggcgag tggcgagtaa
     7381 tttgcgaaac cacccgataa cggagcggcg gtgttctcac gataccgaca ctctcaccag
     7441 atagcagcgc agcaattgcg ctggcagtta ctgcaaactg gcgaagatcg ttttgcccaa
     7501 agggcaacgc tttgccaagt tccaacgtac aggccagcgc gccaaaatgt ctgacgctga
     7561 aatgggtaaa cgtaccacca ggctcctgat ggaacaccag cgcctccagc cccgccgcac
     7621 ccagccacgt cagaaatttc tcgtcccagg gaatgtcgcg ttgcggtaat acaccgaact
     7681 gcggatgcaa ggagccacga attgcggtat gtagatcaag gtgccaacgc acagattctt
     7741 tgccctggtc ataaaaatct tccaggcact gttccagttc gcgcgcccga caggtttctc
     7801 cgctttcagc aaatagctgc caacgaccgc caaacattcg gttcatatcg ctatggcaat
     7861 aacgtttccc ttgcttcagc gcaggaggat tcccgaggat caccagcaac cgccaacgta
     7921 acgggatctc gccgtgagat atcgcgccaa gcaacgcgtc cagcatttcc acaggtgccg
     7981 tctcattacc gtggatcccc gcagaaatca ccagcgcgcc ttgcggtggc gttaatggcg
     8041 tcagttccag cacaccatcg cccagccagc gccagcgaac gccgttgatt tctcgctcgg
     8101 tgataaccgg ttttttaccc gttaaggtca gagcaagaaa attatccatt gccccctccc
     8161 tcgcgctgga acggataaac cgaaccgaga ttcagtaatt ggctcaatac atccagtgct
     8221 tcccgccctt cgcgcagcaa ttgcgggtcg gccaaatcgg cagcagtaag acgatcgcgg
     8281 tagtaacgat ccacccagtc attgagcgca ttaaacaagg tatcgttcat catcaccgcc
     8341 ggattcaccg cccggcgttc ttcttctgtc aataccaccc gcaaccgcag gcacgccggg
     8401 ccgccgccat tcgccatgct ttcacgtaag tcaaagactt ttagttcgct aatcgggttg
     8461 tcagcggcaa ggagttcatt gagataaccc catactccgg cgtgttcccg acactcctga
     8521 ggcagcacca acatcatgga accatcatcg cggctcaaca gttggctgtt aaacagatag
     8581 gtagacaccg catccgacac ggaaacctga gttgccggaa cttctatcgc cataaaaccg
     8641 ttcacccgcg tgccagtaac tgtgactggc gagcgaacgc ctgttggtgg caaaacagca
     8701 cctggcggtt actcacggca atcacgtcat tatgaaaaac gctctggtcg ataacgtccg
     8761 ggttttgctg ggcgaaaatc acctgttggg gattcacctg attaagcctt gccaccgcct
     8821 cgctggcttc gcgagtctgt cgcgccggat aacgggaagg ccgggtatca ttgccttctt
     8881 ctcgcccgta gacaaaaagt agcatacccg gttcaccgta atgaccgccg agacgattgt
     8941 ggtttgccgc cccctcatca ccgagcaacg ctacctgcgg caacgccgaa tggacgctaa
     9001 atttctcttc gtccttaaaa atcgctttta acagcgattc agtgacgggc gcttccagcg
     9061 aacggtgaaa tttattgttc aggttggcaa cggtgagatg cactttgcca tccagcgtat
     9121 cggcagatgg cgcgatcgtt gccgcattgg ctacccacat tggcgaagcg gaactgacgc
     9181 tggaaagcca gtgcggtgcc tggcgtgcaa ctttttccag tacctgctca tcgctaccac
     9241 tgaatcccaa ctgacgcagc accggaataa acggacgctc gtgcggcgga atcacggcct
     9301 gggggaatcc cgcatcggca agggctttca ttttcagtaa gccctgtttt gccgccagtc
     9361 gcgggttaga cacctgaaaa cggtgacggg tagaggcttc attaccaaac gacaggcccg
     9421 cgtaatgatg cgtcagccct accagcccgt cgaaattaac ttcccaggcg ttcatcgcac
     9481 cacctcatcg gaaaaatcca gcccggggtt aagcgtggcg ggcaatgtta acgagtccga
     9541 ctccaggctc gccataggcc atgcgcagta atctgcggca taccaggcgc tggggcgatg
     9601 gttaccggaa gcaccaatgc cgccgaatgg ggcggtactg gcagcaccag taagcggttt
     9661 gttccagtta acaatccccg cccgcgcctc cagcaacagt tgatcgaatt tttcccgctc
     9721 gggggaaacc agaccgcaaa agagtccgaa gcgagtgttg ttcgccatcc gaatcgcctc
     9781 atcaaaagta tcataacgcc agacgcgcaa aaacggcccg aacacctctt catccggcac
     9841 accgccaacg cccgtcattt caatgatccc tggtgtcagc aacgatgtcc ctgcttgtaa
     9901 taagcgcggc gcaagcaggg ttcgtccgcc catcgcttcc agttgctgcc aggcagtaac
     9961 cacctgctgt gcggcctgtt cagaaatcag cccgccaata aacggctgcg gttcgtcatc
    10021 ccagttgccc ggcgttaatc gctggctgac ggcaaccaga ctagcaagaa acgcatcgcc
    10081 ctgcgctccg cttttgagca ataaacggcg ggcgcaggtg cagcgttgcc cggctgtgac
    10141 aaacgccgac tgaatggtca gatggacagc cgcgtcgata tccgccacct catcgataat
    10201 tagcggatta ttaccgccca tctcaagggc gagaattttc tccggctgac cggagagctg
    10261 gcgatgcagc tggtagccgg tattggcgct accggtaaac agcaaaccgt cgagatcctc
    10321 cagcgcactc agcgcctgac ccgtttcacg cccgccctgc accaggttca gcacgcccgg
    10381 cggcaagcca gcctgctgcc ataaacgcat taccgcttcg ccactccacg gtgtcagttc
    10441 gctgggttta aagataatgg tgttacctgc cagcaatgcc ggaacgatat gtccgttcgg
    10501 caaatgacca gggaaattat acggcccaaa caccgccagc acgccgtgcg ggcgatgtcg
    10561 caggctcgcc gcaccgtccg gcatttcact acgctgctcg ccggtacgaa cgtgatacgc
    10621 cttaattgat atcgcgattt tattgatcat cgccgtcact tcggttgccg cttcccagcg
    10681 cggcttaccc gtttctctgg caataatcgc ggttaattcg gctttattgc tttccagcaa
    10741 tccggcaaag cgttcgacaa cgacctgacg ttcagccaat gagagccgcg cccagcgcgg
    10801 aaacgccgca cgggctgccc gacaagcctg ttcgacctga gcggcatcgg catcattgcc
    10861 ttgccataac acctcgcccg ataccggatt acgcttcaca cgcgatgcgc cctggcccgt
    10921 tatccagtca ccgttaatcc ataaagtcat gctgttttct cctctgcgca caggcgcacc
    10981 agacgaacgc gatcccctgc gtggcatttg agggcatcca gttgtgcggc ggttaaaatc
    11041 aaacgctcgg ttgccggatc ggtacgcgcc agcaccacgc ggaaatggtg ataattttca
    11101 ttggcgacca ggcaggccgg aaaatcgccc tgcgcaggct gcccttctgc cacttccacc
    11161 agccgacttt tacggatggc gcgcacgcgg tcgatgtcac actcaagcgt tggcccgccg
    11221 tcgaagatgt cgatatagtt gcggtaacga aaaccttctt tctccagcac cgcgcgggca
    11281 ggcgcggttt gcggatgtac ctgaccgatg acgtcctggg cttcctggga taaaaagtgg
    11341 gtatagatcg gatgtttcgg catcagttct gcaataaatg ccttttgccc ggtgccgcag
    11401 agaaaatcgg cgcggctaaa atccatcgaa aagaagcgtt taccgaggct ttgccagaac
    11461 ggtgaatagc cgtgttcgtc aatcaccccg cgcatttcag caaccacttt gtcattaaac
    11521 ttgtcgcgaa aagccgccat aaacataaag cgcgatttcg acagcaaata gccgttgccc
    11581 tctttgcgcc agtccgggtc gagaaacagc gtgcacagct cgctgctgcc ggtgtgatcg
    11641 ttactaagaa acagcgtcgg caatgcgtta tagacattca gctcttttga ggcgtgaacc
    11701 aacgtgccga cgcgatagtt gtaccaggga tcgttcagcc caaccgccac ctcaatagcg
    11761 caaatccccg ccacggtgcc tgtctcgcta tcttccagca cgaacacata gccctgctcg
    11821 cttttgggca gttcaccttg ccaggttttg attgcccttt cgatacgcgc cgaaagcgtg
    11881 gcttcattgg cgggaagcga cgtcaggccg ccgcccgttt tgctggcaag ctgcatcagc
    11941 gccgagacat ctgagcgctc aacgggacgg atgaccatca tgatgaacct cggctaacaa
    12001 agtgttcgca agcagctgca aagcgatcca gtccggtcgt cacctcttct tcgctgacat
    12061 tgagcgcagg cgcaaaacgc accacgttgc cacccgcaat cagtaccatc acgcctgctt
    12121 tcgccgcttc cagagagagc tgtttcgctt gcccggcgta atcggcattc agtacacagc
    12181 caatcaacaa acctaagccg cgaacttcac tgaacaaacc atagcggtga ttaatagtat
    12241 taagacgctc aacaaaccag tcgtgacgct gtttaacgcc attaagcatc tctggtgtgt
    12301 tgatgagctc cagcactttg cctgctaccg ccgaggccag cggattaccg ccataggtgg
    12361 tgccatgagt gccaacggtc atcacgcggg cgcactcttc ggttgccaac aacgcaccga
    12421 ccgggaaacc gccgcccagc gctttggcgg tggttaacag atcgggcgtc acgccgtagt
    12481 gcatataggc atacagctca ccagtgcgcc cgacgccggt ttgtacctca tcaaaaatca
    12541 gcaaggcatt gtggcggtca cacaattcac gcagaccttg taaaaacgcg ttgctggctg
    12601 gcaccacacc gccttcgccc tagatgggct cgacaatcac cgcacaggtg gcgtcgtcaa
    12661 tcagcgcgct ggcagagtta atatcgttat atgcagcatg acgaatatcc gccggcagtg
    12721 gcgcaaaatc ctgtgaatag gctggctgcc cacccgcact gacagtaaac agcgtgcgac
    12781 catgaaacgc atttttgaac gccacgatgc cactcttatg gctgccgtag cggtcgtgag
    12841 cgaatttacg cgccagtttt agcgccgctt cgttggcttc cgcaccggag ttacaaaaga
    12901 agacgcgatc ggcaaacgtg gcgtcgatca attttttcgc cagtcgcagt accggctcgt
    12961 tggtgtaacc gttgccggta tgccagaact tactcgcctg ttcgttcagc gcttcacgca
    13021 gttccggatg cgcatggccc agcgcgttca ccgcaatgcc acccgcgaag tcgatatact
    13081 ctttcccctg ctgatcccac aagcgcgaac cttcgccacg taccggtata aagggtgccg
    13141 gagcgtaaac aggtatcatc cattcatcaa agttttcacg cgtaattggc tgagacatag
    13201 cgacctctac agtaaataat tcgttatttg tatgttaaca ataagtaatg tttgcgctgt
    13261 aaatgtagat tgcagggttc gtgccagcca gtgataaaag tgcataaacg gcggaggcta
    13321 actggaaatc aaggagttat aaccaaacca tatgcattta aagtgcatat aaagtgaata
    13381 cgtttgcgat gtgggtgaat aaaaaacgca atgttatgca gaagtaaaat ataattctag
    13441 aattgtgatc attgacgaaa tttactggaa attactgcgc cattctgacg cagcgcgcac
    13501 caaaagcggg cattttttgc gccatcgttg acatcattaa caaccatcga tcaaatcact
    13561 taacaacagg cggtaagcaa cgcgaaattc tgctaccatc cacgcactct ttatctgaat
    13621 aaatggcagc gactatgaaa tttgtctctt ttaatatcaa cggcctgcgc gccagacctc
    13681 accagcttga agccatcgtc gaaaagcacc aaccggatgt gattggcctg caggagacaa
    13741 aagttcatga cgatatgttt ccgctcgaag aggtggcgaa gctcggctac aacgtgtttt
    13801 atcacgggca gaaaggccat tatggcgtgg cgctgctgac caaagagacg ccgattgccg
    13861 tgcgtcgcgg ctttcccggt gacgacgaag aggcgcagcg gcggattatt atggcggaaa
    13921 tcccctcact gctgggtaat gtcaccgtga tcaacggtta cttcccgcag ggtgaaagcc
    13981 gcgaccatcc gataaaattc ccggcaaaag cgcagtttta tcagaatctg caaaactacc
    14041 tggaaaccga actcaaacgt gataatccgg tactgattat gggcgatatg aatatcagcc
    14101 ctacagatct ggatatcggc attggcgaag aaaaccgtaa gcgctggctg cgtaccggta
    14161 aatgctcttt cctgccggaa gagcgcgaat ggatggacag gctgatgagc tggggggtgg
    14221 tcgatacctt ccgccatgct aatccgcaaa cagcagatcg tttctcatgg tttgattacc
    14281 gctcaaaagg ttttgacgat aaccgtggtc tgcgcatcga cctgctgctc gccagccaac
    14341 cgctggcaga atgttgcgta gaaaccggca tcgactatga aattcgcagc atggaaaaac
    14401 cgtccgatca cgcccccgtc tgggcgacct tccgccgcta atttagcagc tctcctggct
    14461 caaactgggt caggagaatt aaccttgaga aaaatcaaca aactgtcagt aatgatttgt
    14521 tgcctgccgt cctttgttat accgtcactg cgtttttagt tgtctgacca cttctctatt
    14581 atcaagtttg atataggaaa ctccacgatg aacgctgagc gtaactttct gtttgcctgt
    14641 cttatttttg cgctggtcat ttacgctatc cacgctttcg gtttattcga tctgctcacc
    14701 gatttacccc acttacagac actcattcgc cagagcggat tgttcggtta tagcctctat
    14761 attctgttat tcatcaatgc ccccctcttt ctgttaccag gaagcatact ggtgatcgct
    14821 ggaggaatag tttttggccc gctcttaggg acacaactct cattaattgc cgcaacgctg
    14881 gcctcctcgt gctcattcct gctggcgcgc tggctgggac gtgatttact gctgaaatac
    14941 gtgggccata gccatacctt ccaggccatt gaaaaaggca ttgcgcgtaa cggtattgat
    15001 tttcttattc tgacccgctt aatcccgttg tttccttaca atattcaaaa ttacgcttac
    15061 ggattcacca ccatcgcctt ctggccttat acccttattt cggcactcac gaccctgccc
    15121 ggtattgtta tttataccgt gatggcaagc gatctcgcca atgaaggcat tacgctgcgc
    15181 tttattttac aactctgtct ggcgggcctg gcgctgttta ttctcatcca gctcgcaaaa
    15241 ctactctacg cccgacacaa acatgtggat ctgtctgctt cgcgccgcag cccacttact
    15301 cacccaaaaa atgaaggata gaacgatgtc gcaacattat tcagtgtcat ggaaaaaagg
    15361 actggctgca ctctgcttac tggctgttgc cgggcttagc ggctgcgatc aaaaagagaa
    15421 tgcggcggca aaagtggaat acgacggact ttcgaacagc caaccgctgc gcgtcgatgc
    15481 caataaccat acggtaacca tgctggtgca aattaatggt cgtttcctca ccgacgacac
    15541 tcgtcacggt attgtgttta aagatggctc caacggacat aaatcgctgt ttatggctta
    15601 tgcgcccccg aaagcatttt atgaagccct gaaagaggca ggtggtacgc cgggcgaaaa
    15661 catgacgatg gataataaag aaacgactca tgtcacaggc agcaaactgg atatttcggt
    15721 caactggcaa ggggcggcaa aagcgtattc cttcgatgaa gtgattgttg acagtaatgg
    15781 caagaaactg gacatgcgct ttggcggtaa tttaacggca gcagaagaga agaaaacggg
    15841 ttgcctggtg tgtctggata gctgcccggt cggcatcgtc agcaatgcaa catacactta
    15901 tggtgcggtg gaaaaacacg gcgaagtgaa attcaaaggc aatgcctcag ttctcccggc
    15961 ggataacacg ctggcaacgg ttacctttaa aatcaccgaa taaagccaag ataaaggatg
    16021 atgaagatac aatcgcgtaa aatctggtac taccgtataa ccctcatcat cctgttgttc
    16081 gctatgctgc tcgcatgggc gctgcttccc ggcgtccatg agtttatcaa tcgcagcgtt
    16141 gcggcgtttg ccgccgtgga ccaacagggt atagaacgct ttattcagtc ttacggcgca
    16201 ctggcggcag ttgtctcgtt cttgttgatg attttacagg ccattgctgc accgctacct
    16261 gcgtttttga tcacctttgc caatgcgtcg ctgtttggcg cgttctgggg cggcttgctg
    16321 tcgtggacca gttcgatggc cggcgcggcg ctgtgctttt ttatcgccag agtgatgggc
    16381 cgcgaagtgg tggaaaaatt aaccggcaaa accgtgcttg acagtatgga cggctttttc
    16441 actcgctacg gcaaacacac catcctggtc tgtcggttat tgccttttgt ccctttcgat
    16501 ccaatcagct atgctgccgg tctgacttca atacgttttc gctcgttttt tatcgccacc
    16561 gggcttggtc aattaccggc gactattgtt tattcctggg caggcagcat gttaacaggc
    16621 ggtactttct ggtttgtcac cgaactgttt attctgtttg ccctgaccgt ggtgattttt
    16681 atggcgaaga aaatatggct tgagcgccag aagaggaatg actgatgggt ttaccgccgc
    16741 ttagcaaaat tcctttcatt ttacgtccgc aggcgtggct gcatcgtcgc cattacggcg
    16801 aggtgctaag cccgattcgc tggtgggggc ggatcccgtt tatcttttat ttggtgtcaa
    16861 tgtttgttgg gtggctggag cgcaaacgct caccgctcga tccggtagtg cgatcgcttg
    16921 tcagcgcgcg cattgcgcaa atgtgcctgt gtgagttttg cgtggatatc accagtatga
    16981 aagtcgccga gcgtaccggc agcaccgata aactgctggc agtggctgac tggcggcaaa
    17041 gcccgctctt tagcgatgaa gaacggctgg cgctggagta cgccgaagcc gcaagcgtca
    17101 caccgccaac agtcgatgat gccctgcgta cccgactggc tgcgcatttt gacgctcagg
    17161 cgctcaccga actgacggca ttgatcggcc tgcaaaacct gtcggctcgt tttaattctg
    17221 ccatggacat tcccgctcag gggctgtgcc gtattccgga aaaacgttct taaggaggga
    17281 agatgcgcca ttgtgggtgg ttgctgggat tgttatcgct gttttctttg gcaacacatg
    17341 ccagtgactg gcaggaaatt aaaaatgagg ccaaagggca aaccgtctgg tttaacgcct
    17401 ggggcggcga taccgcaatt aaccgctatc tcgactgggt tagcggcgag atgaaaaccc
    17461 attacgctat aaacctgaag attgttcgtc tcgcggatgc cgcagacgcg gtgaagcgca
    17521 ttcagaccga agctgctgcc ggtcgtaaaa cgggcggctc ggtggatctg ctctgggtga
    17581 acggcgaaaa cttccgcacc ttaaaagagt aatgccagtc agttaagcaa ctgactggca
    17641 tt
//
LOCUS       DAZSNM010000075        17640 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZSNM010000075 DAZSNM010000000
VERSION     DAZSNM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17640
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.075"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            36..719
                     /locus_tag="ACHW42_002071"
     CDS             36..719
                     /locus_tag="ACHW42_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR8943893.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            763..5425
                     /locus_tag="ACHW42_002072"
                     /pseudo
     CDS             763..5425
                     /locus_tag="ACHW42_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            5793..7448
                     /gene="lldP"
                     /locus_tag="ACHW42_002073"
     CDS             5793..7448
                     /gene="lldP"
                     /locus_tag="ACHW42_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR8943894.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7448..8224
                     /gene="lldR"
                     /locus_tag="ACHW42_002074"
     CDS             7448..8224
                     /gene="lldR"
                     /locus_tag="ACHW42_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR8943895.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8221..9411
                     /gene="lldD"
                     /locus_tag="ACHW42_002075"
     CDS             8221..9411
                     /gene="lldD"
                     /locus_tag="ACHW42_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR8943896.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9607..10080
                     /gene="trmL"
                     /locus_tag="ACHW42_002076"
     CDS             9607..10080
                     /gene="trmL"
                     /locus_tag="ACHW42_002076"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR8943897.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10133..10954)
                     /gene="cysE"
                     /locus_tag="ACHW42_002077"
     CDS             complement(10133..10954)
                     /gene="cysE"
                     /locus_tag="ACHW42_002077"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR8943898.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11034..12053)
                     /gene="gpsA"
                     /locus_tag="ACHW42_002078"
     CDS             complement(11034..12053)
                     /gene="gpsA"
                     /locus_tag="ACHW42_002078"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR8943899.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12053..12520)
                     /gene="secB"
                     /locus_tag="ACHW42_002079"
     CDS             complement(12053..12520)
                     /gene="secB"
                     /locus_tag="ACHW42_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR8943900.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12583..12834)
                     /gene="grxC"
                     /locus_tag="ACHW42_002080"
     CDS             complement(12583..12834)
                     /gene="grxC"
                     /locus_tag="ACHW42_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR8943901.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12976..13407)
                     /locus_tag="ACHW42_002081"
     CDS             complement(12976..13407)
                     /locus_tag="ACHW42_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR8943902.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13652..15196
                     /gene="gpmM"
                     /locus_tag="ACHW42_002082"
                     /gene_synonym="pgmI"
     CDS             13652..15196
                     /gene="gpmM"
                     /locus_tag="ACHW42_002082"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR8943903.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15230..16489
                     /gene="envC"
                     /locus_tag="ACHW42_002083"
     CDS             15230..16489
                     /gene="envC"
                     /locus_tag="ACHW42_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR8943904.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16493..17452
                     /locus_tag="ACHW42_002084"
     CDS             16493..17452
                     /locus_tag="ACHW42_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR8943905.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17439..17640)
                     /locus_tag="ACHW42_002085"
                     /pseudo
     CDS             complement(17439..17640)
                     /locus_tag="ACHW42_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4395 a         4590 c         4632 g         4023 t
ORIGIN      
        1 attgagcgct ggtcggagga aataaggatt tttacatgaa cttgaagaag atttttttca
       61 gtgctgtcac tgtttcagtt ctctgcgcat taacgggatg tgactacatt gaagaaggca
      121 agccagaaag tagtttactg aaacaacaag aagaacataa taataaaatt gtgctacttg
      181 aaaaacaaca agcacagcta aagagtcaac tcgaaactat tcaaaaacaa caaactggca
      241 taataaacag tacaaagact ttaactcacg taattaaatc cgttaaagac caacaaaata
      301 cttttatttt taccgagttt aacccagcaa aaacgaagta tttcatcctg aacaacggtt
      361 cggtggcttt agcgggtcga gtgttatcca ttgacgccac agagaatggt agtgttattc
      421 atatctcact ggtcaactta ttaagtacac ctatctcaaa cattggtttt aatgcgacat
      481 ggggtggtga aaaacctgtc gatgccaaag agtttgcccg gtggcaacaa ttacttttca
      541 acacatcaat gaaatccaca ttgaaattat taccaggtca atggcaagac attaatttga
      601 ccctgaaggg tgtatcgccc aataacctgg gatatctgaa gttagccatc aacatggaaa
      661 atattcagtt cgacaatctt ccctctgctg aaaatcggca gaaaagaagc aaaaaataaa
      721 tgcagttagt acgcttataa caaggaatag cgagcatcat taatgaacaa aatatttaaa
      781 gttatctgga accctgcgac agggaattat actgttacca gcgaaacggc aaaaagccgt
      841 ggcaagaaat ctgggcgcag taagctgtta atttctgcgc tggttgcggg tggaatgttg
      901 tcgtcgtttg gggcattggc gaatgccggg aatgacaacg gtcagggtgt tgattacggt
      961 agtggatcag ctggcgacgg ctgggttgct ataggcaaag gggcgaaagc aaatactttt
     1021 atgaacacca gtggttccag tactgctgtg ggttatgacg ctatagctga aggccaatat
     1081 agctctgcca tcgggtcaaa aacccatgcg attggtggtg catcaatggc ctttggggtt
     1141 agtgcaatat cagaaggcga tagaagtata gcactgggtg cctcttcgta ttcattgggc
     1201 caatactcaa tggccctcgg ccgttattca aaagcattgg gtaaattgtc tattgctatg
     1261 ggggactctt ccaaagcgga aggagcaaac gccattgccc tggggcgtag cagtgtagct
     1321 agcggtactg acagcctcgc atttggcaga caatcacttg ccagcgcagc gaacgctatt
     1381 gcgataggtg ctgagaccga agccgctgaa aatgcaactg ctattggcaa taatgcgaag
     1441 gcaaaaggga ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa agtcaatact
     1501 atcgcattag gaaatggcag ccaggctctg gcagataatg caatcgccat aggccagggc
     1561 aacaaagctg atggcgtgga tgccatcgct ctgggtaatg gtagccagtc gagaggctta
     1621 aacaccattg ccttaggcac agccagtaat gcaactggtg ataagagtct tgcgcttggt
     1681 agtaatagca gtgccaacgg tattaactct gtcgcgctgg gcgcagattc cattgcggat
     1741 ttagacaata ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt taatgtgaaa
     1801 aatggcgcga tcaagtctga cagttacgat gccattaatg gttcacagct ttatgccatt
     1861 agcgactcgg tagcaaaaaa ggcttggagg aggggctgca gtagatgttg atgacggtac
     1921 tgttacagca ccaacctaca atttaaaaaa tggtagcaaa aataacgtag gggctgcgct
     1981 cgctgtactt gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat acagcgctgc
     2041 tcatggtact agtagcccaa ctgccagcgt aatcaccgat gttgcggatg gcacgatttc
     2101 agcctccagt aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg acgatgtcga
     2161 agccaacacc accaatatca ccaacctgac ggattccgtt ggtgaccttc aggctgatgc
     2221 cctgctctgg aacgaaacta aaaaggcatt cagtgcagct cacggccagg ataccaccag
     2281 caaaatcacc aacgttaaag atgccgacct gacggctgac agcactgatg ctgttaacgg
     2341 ctctcagctg aaaaccacca acgatgctgt ggcgacgaat accaccaata tcgccaataa
     2401 cacttccaat attgccacta acaccaccaa catctctaac ctgactgaga cggtgactaa
     2461 tcttggtgag gatgcgctga aatgggataa ggacaatggt gtattcacgg cagctcatgg
     2521 caccgagacc accagcaaaa taaccaacgt taaagatggc gacctgacga ctggcagcac
     2581 cgatgccgtt aacggctctc agctgaaaac caccaacgat gccgtggcga cgaataccac
     2641 caatatcgcc actaacacca ccaacatctc taatctgact gagacggtga ctaatcttgg
     2701 tgaggatgcg ctgaaatggg ataaggacaa tggtgtcttc actgcagctc atggcaacaa
     2761 taccgccagc aaaatcacca atatcctgga cggcacagtc actgcaacca gttccgatgc
     2821 cattaacggt agccagcttt atgacttaag cagcaatatc gccacctact tcggcggcaa
     2881 tgcttctgtg aatactgacg gtgtgtttac cggtccaacc tacaaaatcg gtgaaacaaa
     2941 ttattataac gtcggcgatg cactggctgc gattaactcc tcatttagca cgtctctcgg
     3001 cgatgctctg ctttgggatg ccaccgcagg taaattcagt gccaaacacg gtactaatgg
     3061 tgacgcaagc gtgatcactg atgtcgcaga tggtgaaatt tcagactcca gttctgacgc
     3121 agtaaacggc tcacaactcc acggcgtgag cagttatgtt gttgatgcgc tggggggtgg
     3181 tgccgaagtc aatgcagacg gcaccatcac tgcgccgacg tacaccattg ctaatgctga
     3241 ttacgataat gtcggtgatg ccctgaatgc tatcgatacc actcctgacg acgctctgct
     3301 ctgggatgcg gacgccggtg aaaatggtgc atttagcgcc gctcacggaa aagataaaac
     3361 tgccagtgta atcactaacg tcgctaacgg tgcaatctct gctgccagca gcgacgcgat
     3421 taacggctca caactctata ccaccaataa gtacatcgct gatgcgctgg gtggtgacgc
     3481 agaagtcaac gctgacggca ccatcaccgc accgacttac accattgcga acgccgagta
     3541 caacaacgtc ggtgacgccc tggatgcgct tgatgataac gccctgctgt gggataagac
     3601 tgccaatggc ggtgctggag cctacaatgc cagccatgac ggtaaagcca gcatcatcac
     3661 taatgtcgct aatggcagta ttagtgagga cagtaccgat gcagtgaacg gttctcagtt
     3721 gaatgcgacg aatatgatga ttgagcagaa cacccaaatt atcaatcagc tcgctggtaa
     3781 caccgacgca acctatatcg aagaaaatgg cgctggtatc aactacgttc gtactaacga
     3841 caacgattta gcctttaacg atgcaagcgc ctctggtgtc ggcgctacag ctgtaggtta
     3901 taacgctgtc gcgtctggtg ccagcagcgt agccattggt cagaacagca gcagcaccgt
     3961 tgataccggt attgcgctgg gtagcagctc cgtttccagc cgtgtgattg ccaaaggttc
     4021 tcgtgacact agcgtaacgg aaaatggcgt ggtgattggt tatgacacca ctgacggcga
     4081 actgctaggt gcattgtcaa ttggtgatga cggtaaatac cgccaaatca tcaacgtagc
     4141 tgatggttca gaagcccatg acgccgttac ggttcgccag ttgcagaacg ctattggagc
     4201 ggtcgccact acgccaacca agtactttca cgccaactca acggcagaag actcactggc
     4261 cgttggtgaa gactcgctgg caatgggtgc gaaaactgtc gttaatggta atgcaggtat
     4321 tggtatcggt ttgaacactc tggttctggc tgatgcgatc aacggcattg ctatcggcag
     4381 taacgcacgc gcaaaccatg caaacagtat cgcaatgggt aacggttctc agaccacccg
     4441 tggtgcacag actggctaca ccgcctacaa catggacgca ccgcagaact ctgtaggtga
     4501 gttttctgtc ggtagtgaag acggtcaacg tcagatcaca aacgtcgcag ctggttcagc
     4561 ggataccgat gcggttaacg tgggtcagtt gaaagtcact gatgagcgcg tagcgcaaaa
     4621 tacccagagc attactaacc tgaacaatca ggtcactaat ctggatactc gcgttactaa
     4681 tatcgaaaac ggtattggcg acattgtcac caccggtagc accaagtact tcaagaccaa
     4741 caccgatggc gtagatgcca acgcccaggg taaagatagc gttgctattg gttctggttc
     4801 cattgctgcc gctgacaaca gcgtcgcact gggtaccggt tccgttgcag aggaagaaaa
     4861 tacaatctct gtaggttctt ctactaacca acgccgtatc accaacgtag ctgccagtgt
     4921 taatgccacc gatgcggtta acgtgtcgca gctgaaatct tctgaagcag gcggagtgcg
     4981 ctacgacacc aaagctgatg gttctatcga ctatagcaat atcaccctcg gtggtggcaa
     5041 cggcagtacg actcgtatca gcaacgtctc cgctggcgtc aacaacaacg acgcggtgaa
     5101 ctacgcgcag ttgaagcaaa gcgcgcagga aacgaagcaa tacaccgatc agcggatggt
     5161 tgagatggat aacaaactgt ctaaaactga aagcaagttg agcggtggta tcgcttctgc
     5221 aatggcaatg accggtctgc cgcaggctta tacaccgggt gccagcatgg cttctattgg
     5281 tggcggtact tacaacggtg aatcggcagt tgctttaggt gtatcgatgg tgagcgccaa
     5341 tggtcgttgg gtctacaaat tacaaggtag taccaatagc cagggtgaat actccgccgc
     5401 actcggtgcc ggtattcagt ggtaatgatc cattaagtta gtgtgactaa gggcaaacgc
     5461 caaattgcct gatgcgttac gcgcatcagg caagataagg cgctccgggt caacaatcta
     5521 agggcaattc tctgatgagg attgcccttt tctttaccag acatctcccc ccacaagaat
     5581 tggccctacc aattcttcgc ttatctgacc tctggttcac aattccccaa ttaaaactca
     5641 catcaatatt gccaatacat aacatttagt taaccattca ttgtcattat ccctacacaa
     5701 cacaattggc agtgccactt ttacacaacg tgtgacaagg agatgagcaa cagactcatt
     5761 acacgatgtg cgtggactct aggagacctg caatgaatct ctggcaacaa aactacgatc
     5821 ccgccgggaa tatctggctt tccagtctga tagcatcgct tcccatcctg tttttcttct
     5881 ttgcgctgat taagctcaaa ctgaaaggat acgtcgccgc ctcatggacg gtagcaatcg
     5941 cccttgccgt ggctttgctg ttctataaaa tgccggtcgc taacgcgctg gcctcggtgg
     6001 tttatggctt cttctatggt ttgtggccca tcgcgtggat cattattgca gcagtgttcg
     6061 tctataagat ctcggtgaaa accgggcagt ttgacatcat tcgctcgtct attctttcga
     6121 taacccctga ccagcgtctg caaatgctga tcgtcggttt ctgtttcggc gctttccttg
     6181 aaggagccgc aggctttggc gcaccggtag caattaccgc cgcattgctg gtcggcctgg
     6241 gttttaaacc gctgtacgcc gccggactgt gcctgattgt taacaccgcg ccagtggcat
     6301 ttggcgcgat gggcattcca atcctggttg ccggacaagt aacaggcatc gacagctttg
     6361 agattggcca gatggtgggg cgtcagctac cgtttatgac cattatcgtg ctgttctgga
     6421 tcatggcgat tatggacggc tggcgcggta tcaaagagac gtggcctgcg gtcgtggttg
     6481 caggcggctc gtttgccatc gctcagtacc ttagctctaa cttcattggg ccggagctgc
     6541 cggacattat ctcttcgctg gtatcactgc tctgcctgac gctgttcctc aaacgctggc
     6601 aaccagttcg cgtattccgc ttcggtgact tgggggcgtc acaggttgat atgacgctgg
     6661 cccacaccgg ttacaccgcg ggtcaggtgt tacgtgcctg gacaccgttc ctgttcctga
     6721 ccgctaccgt aacactgtgg agcatcccgc cgtttaaagc cctgttcgca tcgggtggtg
     6781 cgctgtatga gtgggtgatc aacattccgg tgccgtacct caataaactg gttgcccgta
     6841 tgccaccagt ggtcagcgag gctacagcct atgccgccgt gtttaagttt gactggttct
     6901 ctgccaccgg caccgccatt ctgtttgctg cactgctctc gattgtctgg ctgaagatga
     6961 aaccgtctga cgctatcagc accttcggca gcacgctgaa agaactggct ctacccatct
     7021 actccatcgg catggtgctg gcgttcgcct ttatctcgaa ctattccgga ctgtcatcaa
     7081 cactggcact ggcactggcg cacaccggtc atgcattcac cttcttctcg ccgttcctcg
     7141 gctggctggg ggtattcctg accgggtcgg atacctcatc taacgccctg ttcgccgcgc
     7201 tgcaagccac cgcagcacaa caaattggcg tctctgatct gttgctggtt gccgccaata
     7261 ccaccggtgg cgtcaccggt aagatgatct ccccgcaatc tatcgctatc gcctgtgcgg
     7321 cggtaggcct ggtgggcaaa gagtctgatt tgttccgctt tactgtcaaa cacagcctga
     7381 tcttcacctg tatggtgggc gtgatcacca cgcttcaggc ttatgtctta acgtggatga
     7441 ttccttaatg attgttttac ccagacgcct gtcagacgag gttgccgatc gtgtgcgggc
     7501 gctgattgat gaaaaaaacc tggaagcggg catgaagttg cccgctgagc gccaactggc
     7561 gatgcaactc ggcgtatcac gtaattcact acgcgaggcg ctggcaaaac tggtgagcga
     7621 aggcgtgctg ctcagtcgac gcggcggcgg gacgtttatt cgctggcgtc atgacacatg
     7681 gtcggagcaa aacatcgtcc agccgctaaa aacgctgatg gccgatgatc cggattacag
     7741 tttcgatatt ctggaagccc gctacgccat tgaagccagc accgcatggc atgcggcaat
     7801 gcgcgccaca cctggcgaca aagaaaagat tcagctttgc tttgaagcaa cgctaagtga
     7861 agacccggat atcgcctcac aagcggacgt tcgttttcat ctggcgattg ccgaagcctc
     7921 acataacatc gtgctgctgc aaaccatgcg cggtttcttc gatgtcctgc aatcctcagt
     7981 gaagcatagc cgtcagcgga tgtatctggt gccaccggtt ttttcacaac tgaccgaaca
     8041 acatcaggct gtcattgacg ccatttttgc tggtgatgcc gacggggcgc gtaaagcaat
     8101 gatggcgcac cttagttttg ttcacaccac catgaaacga ttcgatgaag atcaggctcg
     8161 ccacgcacgg attacccgcc tgcccggtga gcataatgag cattcgaggg agaaaaacgc
     8221 atgattattt ccgcagccag cgattatcgc gccgcagcgc aacgcattct gccgccgttc
     8281 ctgttccact atatggatgg tggtgcatat tctgaataca cgctgcgccg caacgtggaa
     8341 gatttgtcag aagtggcact gcgccagcgt attctgaaaa acatgtccga cttaagcctg
     8401 gaaacgacgc tgtttaatga gaaattgtcg atgccggtag cactggcacc ggtgggtttg
     8461 tgtggcatgt atgcgcgtcg tggcgaagtt caggcagcca aagcggcgga cgcgcatggc
     8521 attccgttta ctctctcgac ggtttccgtt tgcccgattg aagaagtcgc gccagccatc
     8581 aagcgcccaa tgtggttcca gctttatgta ctgcgcgatc gcggctttat gcgtaacgcg
     8641 ctggagcgag caaaagcagc gggttgttcg acgctggttt tcaccgtgga tatgccgaca
     8701 ccgggcgcac gctaccgtga tgcgcattca ggtatgagcg gcccaaatgc ggcaatgcgc
     8761 cgctacttgc aggcggtgac gcatccgcaa tgggcgtggg atgtgggcct gaacggtcgt
     8821 ccacatgatt taggtaatat ctcagcttat ctcggcaaac cgaccggact ggaagattac
     8881 atcggctggc tggggaataa cttcgatccg tccatctcat ggaaagacct tgagtggatc
     8941 cgcgatttct gggatggccc gatggtgatc aaagggatcc tcgatccgga agatgcgcgc
     9001 gatgcagtac gttttggtgc tgatggaatt gtggtttcta accacggtgg ccgccagctg
     9061 gacggtgtac tctcttccgc tcgtgcgttg cccgctattg cagatgcggt gaaaggtgat
     9121 atcgccattc tggcggatag cggaattcgt aacgggcttg atgtcgtgcg tatgattgcg
     9181 ctcggtgccg acaccgtact gctgggtcgt gctttcctgt atgcgctggc aacagcgggc
     9241 caggcgggtg tagctaacct gctaaatctg atcgaaaaag agatgaaagt ggcgatgacg
     9301 ctgactggcg cgaaatcgat cagtgaaatt acgcaagatt cgctggtgca ggggctgggt
     9361 aaagagttgc ctacggcact ggctccgatg gcgaaaggga atgcggcata gtcgtttgcg
     9421 ccccctcacc ctaaccctct ccctcaggga gaggggaccg ttcggcgctg tatgtactcc
     9481 ctcactctga aacgacaccg cactcttttt ttctccctcg cccctccggg gagggccggg
     9541 gtgaggggaa aaggccgcac ggttcaccaa ttttctgcta tcctgccccc gcactaaggg
     9601 ggcagtatgc taaacatcgt actttacgaa ccagaaattc cgccaaatac tggcaacatc
     9661 atccgtcttt gcgctaatac cggctttcgt ctacatatca tcgaaccgat gggatttgcc
     9721 tgggacgaca agcgcctgcg ccgcgcgggg ctggactatc acgagtttac cgccgttacg
     9781 cgtcatcatg actatcgcgc gttcctcgaa gcagaaaatc cccagcgcct gtttgccctc
     9841 accacgaaag gtacgcctgc tcacagcgcc gtaagctatc aggatggcga ttatctgatg
     9901 ttcggcccgg aaacacgcgg cctgccagcg agcattcttg atgccctgcc cgctgaacaa
     9961 aaaattcgca ttccgatggt gccggacagc cgcagcatga atctgtccaa tgcggtgtcg
    10021 gtagtggtgt atgaagcctg gcggcagctg ggatatccgg gagcgttgtt gagagattag
    10081 ttactgtatg ccggatagat gattacatcg catccggcac gatcacagga cattagatcc
    10141 catccccata ctcaaatgta tggttaatac cgttgaaatg ctggtccata tccattgatg
    10201 gcttatcgct gtctggttta ccgacaatac gagccggaac gccagcggcg gttgtatgcg
    10261 gcggcaccgg ttgcagcacc acggaacctg cgccaatctt cgcgccgcga ccaacttcaa
    10321 tattgccgag gattttcgcg cccgcgccaa tcatcacacc ttcacgaatt ttcgggtgac
    10381 ggtcaccacc agatttaccc gtaccgccaa gcgtcacaga ttgcagaatc gatacgtcgt
    10441 tttcaattac cgccgtttca cccacaacga tgcccgtggc gtggtcgagc ataatgccac
    10501 ggccaatttt tgctgccggg tgaatatcga cctggaacgt cacagaaacc tggttttgca
    10561 gaaagattgc caatgcgcga cgcccctgat tccacaacca gtgaccgatg cgataggcct
    10621 gcaaggcatg aaaacccttc aggtataaca acggggttga gtatttatcg actgccgggt
    10681 cgcgggtacg caccgcctga atatcacagg ccgcagaggc gatcatttcc gggtcagcgg
    10741 cgtaggcttc ttcaaccact tcacggatag caatagcagg cataattggc gatgacagct
    10801 tgttcgccag catgtagctc agtgcactgc caaggttttc gtgcttgagt agcgtcgcgt
    10861 ggtaaaaact ggccagcatt ggctcacagt ccgccagcgt tctggcttcg gctttaatat
    10921 tgttccagac aatttccagt tcttcacacg acattgctta ctccacacga tgagataatg
    10981 accggcccgt tctgcgcggg ccgggtcata gcggtaacaa aggttccctg gggttagtga
    11041 ctgctgcgct cgtccttgcg tgcacgacct aataatgtca atgctgcctc gcgcgcgttt
    11101 tttccgcaat ataatacttg ataaatttcc tcggttattg gcatttcaac gccgaagcga
    11161 tgcgccagtt cgcggacttc tttcgtattg cggtagcctt ccaccacctg accaatcttc
    11221 tcctgcgcgc tttgtacatc catgccctga ccgagcatca taccaaaacg gcggttacgc
    11281 gactggttgt cggtacaggt aagcaccaga tcgcccagcc ccgccatgcc cataaaggtg
    11341 gcaggatcgg cacccagcgc cgcaccaaga cgcgacattt cagccagccc acgggtgatc
    11401 agcgccgtac gcgcattcgc accaaaaccg ataccgtcag acatccccgc gccaatggca
    11461 atgacgtttt tcaccgcgcc gccaagctgc acgccaatga aatccgggtt gctgtaaacg
    11521 cggaaacttt tgccgcagtg cagcagctgc tggagatcat cggcaaaggt ctgatcggtc
    11581 gaggccagcg aaatagctgt cggtaaacct gccgccagtt ctttcgcaaa cgttgggcca
    11641 gagataaccg ccagcggaat ttgatcgcct aaggcctcac gcgccacgtc ctgtaacaga
    11701 cgtccggttt ccgcttccag ccctttggtc gcccacacca gacgcgcatc aggacgcatc
    11761 agcggtttaa tctggcgcag cacttcacca aagacatggc tgggtacgac gacgagaata
    11821 ttacgactgg ctgccagcgc agtggcgaga tcgctttcaa gatggagcgt atcgggaaaa
    11881 ggcacatcgg ggagaaacgc ggcgttacag cggtcgcgtt caagcgttgc gatatgttca
    11941 gggtcatggc cccagaggac aacctcgtgg ccatttcttg ccagggtaat ggcaagagcg
    12001 gtgccgtacg agccggcacc gatcacagtc attgaagcat tacgttggtt catcaggcat
    12061 cctgatgttc ttcagtacct tcgccagcct gctgctgcaa atagttcatg aacagcgcat
    12121 cgaagttaac cggcgcaagg ttcagttgcg ggaatgtacc gcgggatacc atgctggtga
    12181 tgcactcacg agcatacggg aacagaatgt tcgggcagta tgcgcccagg caatgtgcca
    12241 tctgggtgcc ttcgataccc gcgatggaga aaataccgcc ctgctgaact tcacacagga
    12301 acgcggtttc ttcgcccaag gaggccgtta cggtaacacg cagtaccact tcgtatacgt
    12361 catctgccag ttgggaagat gccgtatcca gatcaagttt aacttctggt tgccaatctt
    12421 tctggaaaac gtgcggcgcg ttcggcgctt cgaaagagat atccttggta taaatacgtt
    12481 ggatctggaa agtcatttca gtgttgtttt gttctgacat gtgtagaaaa cccttaagtg
    12541 ttgtccttaa atactgcgca atgtaatacc aacgttcaca cgttatttca gcaggggatc
    12601 cagtccacca cgtgcatcca atgcatacaa gtcatcacag ccgccaatgt gctgtgcgtc
    12661 aataaaaatc tggggaaccg tggtgcaacc gctgcgtttg atcatctctt cacgcttggc
    12721 ggcattgcca tcgatcggca actcctggaa actcacgccc ttgctgctca gcagtgcttt
    12781 tgcacgatgg caatacgggc aggtttcttt ggtatagatt tcaacattgg ccataattta
    12841 gctcctgttt ttttaccctg aaatttcatg ttgcagggcg gctgcaaaat ggagtgtccc
    12901 cagaagctta taacaataag tcgctggggg cctaattgca gctaacggcc tgcatcatga
    12961 aagacgacag gtaaattatt tgccgcgcac cagaggcaag ttttcgccag cccagccagc
    13021 aacgccctct ttcagcacga atacttgcgc aaaaccagct ttcgtcagcg cgtttgcagg
    13081 ctcctggcac tgcatgccag aaccgtctac cacgataacc gatttgtctt tgtgcttctc
    13141 aagctcacca acattgttgg ctttgatttc gctcggcaac agattgatag aacctgcgat
    13201 atggccttta cggaagtcat cacgctgacg taaatccaca accacagcgt cttctttgtt
    13261 gatcagacgc gtagcttcac cacgagtaat caccttcact ttcgaggtca ggctcttaaa
    13321 cgtagtcaca agaaccgcca ccagtaacgc gatccaggcg atactcagta tgggatgacg
    13381 gccaacaaat tgcataattt cttgcatggg gggtaacaac tcccgacgta gtgattaaaa
    13441 aaaccaggaa aggagtatac ctgcgcggtg cggcaaatac agccagcgcg ttaactggaa
    13501 tgcaattttg cggggcacga cgaaaaaaaa gcgcaaatct ggcatcggct tgccgcgagc
    13561 ggtcgtatat tttgatcttc agaggctatt ttatcgattc agctgtagta aaattacgca
    13621 aattttgact cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac ctatggtact
    13681 ggtgattctg gatggctatg gctatcgtga agaacagcag gataacgcca tttttagtgc
    13741 taaaaccccg gtaatggatg cactgtgggc caatcgtccg cataccctga tcgacgcttc
    13801 cggtctggaa gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag gccacgttaa
    13861 cctgggtgcc ggccgcatcg tgtatcagga cctgactcgt ctggacgttg aaatcaaaga
    13921 tcgcgctttc tttgctaatc cagtgctggc tggcgcggta cataaagcga aaaacgcagg
    13981 taaagcagta cacattatgg gtctgctctc cgcaggcggc gtacacagcc acgaagatca
    14041 catcatggcg atggtagaac tggcagctga acgcggcgca gaaaaaatct acctgcacgc
    14101 attccttgac ggtcgcgaca ccccgccgcg cagtgctgaa tcctcgctga aaaaatttga
    14161 agagaaattt gctgcgctgg gtaaaggccg cgtagcgtcc atcattggtc gttactacgc
    14221 gatggaccgc gataaccgtt gggatcgcgt tgaaaaagcc tatgacctgc tgactctggc
    14281 gcagggtgag ttccaggccg atactgccgt tgctggtttg caggcagctt atgctcgcga
    14341 cgaaaacgac gagttcgtga aagcgaccgt tattcgtgca gaaggtcagc cagatgcggc
    14401 aatggaagac ggcgatgcgc tgattttcat gaacttccgt gctgaccgcg cgcgtgaaat
    14461 cactcgtgca ttcgtgaacg ctgatttcga tggcttcgcg cgtaagaaag tggttaacgt
    14521 cgatttcgtg atgctgaccg aatacgccgc tgacatcaaa actgcggttg cttacccacc
    14581 cgcttccctg gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa ctcagttgcg
    14641 tatttccgaa accgaaaaat atgcccacgt tactttcttc ttcaacggtg gcgtagaaga
    14701 gtcgttcaaa ggcgaagatc gcattctgat caactcaccg aaagtggcta cctacgatct
    14761 gcaaccggaa atgagctccg cagagctgac cgaaaaactg gttgcggcca tcaagagcgg
    14821 caaatacgac accattatct gtaactatcc gaacggcgac atggtaggtc acaccggggt
    14881 gatggaagcg gcggttaaag cggttgaagc actggatcac tgcgtggaag aagtcgcgaa
    14941 agcggttgag tccgtgggtg gacaactgct gatcaccgct gaccacggta acgctgaaca
    15001 gatgcgcgat ccggcaacgg gtcaggcaca cacggcacac accaacctgc cagttccgct
    15061 gatttacgtt ggtgataaga acgtgaaagc ggttgcaggc ggcaaacttt ctgacatcgc
    15121 gccgaccatg ttgtcgctga tgggtatgga aatcccgcaa gagatgactg gtaagccgct
    15181 gttcatcgtg gaataatccc tccccatgag gggaaaggcg attaatacca tgacacgggc
    15241 cgtgaaaccg cgcaggtttg caatcaggcc catcatctac gccagcgttc ttagcgctgg
    15301 cgtattgttg tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca aatctattca
    15361 ggccgatatc gccgcaaaag agcgcgcggt acgccaaaag caacaacaac gcgcaagcct
    15421 gctcgcacaa ttgaaaaagc aggaagaagc gatctctgaa gccacccgta agctgcgcga
    15481 aacgcaaaac acgctcaatc aactcaataa acagattgat gagatgaacg cgtcgattgc
    15541 caaactggag cagcaaaaag ccgcccagga gcgcagcctc gccgcacaac tggatgccgc
    15601 attccgtcag ggcgagcata ccggtattca gctaattctc agcggtgaag aaagccagcg
    15661 tggacagcgt ttacaggctt atttcggcta tctcaaccag gcgcgacaag aaaccattgc
    15721 ccagttgaag caaacgcgtg aagaagtcac catgcagcat gctgaactgg aagagaaaca
    15781 aagcgagcaa caaacgctgt tatatgaaca gcgcgcccaa caggcgaaac tgactcaggc
    15841 tctgaacgag cgtaaaaaga cgctggcggg gctggaatct tccatccagc aaggtcagca
    15901 gcagctaagt gaactgcgtg ccaacgaatc ccgtctgcgt aacagcattg cccgtgctga
    15961 agccgcggcg aaagcgcgtg ctgaacgaga agcgcgtgag gcccaggcgg ttcgcgagcg
    16021 ccagaaagaa gcgacgcgca aaggcaccac ctacaaaccg accgaaagcg aaaaatcgct
    16081 gatgtcccga actggtggcc tgggtgcgcc gcgtggtcag gcattctggc cggttcgcgg
    16141 gccgacgctg catcgctatg gcgagcagct acagggtgaa ttacgctgga aagggatggt
    16201 tatcggtgcc tctgaaggta ctgaagttaa agcaattgcc gacggtcgag tgattctggc
    16261 tgactggctg caaggttacg gtctggtggt ggtggttgag cacggtaaag gcgacatgag
    16321 tctttacggc tataatcaga gcgcactggt gagcgttggt tcgcaggttc gcgcgggcca
    16381 gccaattgca ctggtgggca gcagtggcgg tcagggtcgg ccttcactct atttcgaaat
    16441 tcgccgccag ggtcaggcgg tcaatccaca gccgtggttg ggaagataag ttttgtttcc
    16501 atttcgtcgt aacgttcttg catttgccgc tctgttggcg ctctcctccc ccgtacttgc
    16561 tggcaaactt gccatcgtca ttgatgattt tgggtatcgc ccgcacaacg aaaaccaggt
    16621 gctggcgatg ccttccgcta tctccgtcgc tgtattaccc gattcaccgc acgccagaga
    16681 gatggcgacc aaagcgcata acagcggtca cgaagtgttg attcatctcc cgatggcacc
    16741 gttgagtaaa cagccgctgg agaaaaatac gctacgcccg gagatgagca gcgacgaaat
    16801 tgagcgcatt attcgtagtg cggtcaataa cgtgccctat gccgtgggga tcaacaacca
    16861 catgggcagc aagatgacct ctaacctgtt tggtatgcag aaagtgatgc aggcgctgga
    16921 gcgttacaat ctttacttcc tcgacagcgt aaccatcggt aatacccagg cgatgcgcgc
    16981 cgcgcaaggt actggcgtga aggtgatcaa acggaaggtg ttcctcgacg attcgcagaa
    17041 cgaagcggac atccgtgtgc aatttaatcg cgcaattgat ctggcgcgtc gcaacggttc
    17101 aaccattgcc attgggcatc ctcacccttc aacggtacgc gtgctacaac agatggttta
    17161 taacctgccg ccagacatta cgctggtgaa agccagcagc ttgctgaatg aaccgcaggt
    17221 tgatacttct acaccgccga aaaacgctgt gcctgatgca ccgcgtaatc cgttccgtgg
    17281 cgtgaagctg tgcaaaccga agaaaccgat agaacctgtt tatgctaatc gcttctttga
    17341 agtattaagc gaaagcatca gccagagcac gctgatcgtt tacttccagc atcagtggca
    17401 aggctggggc aaacagcccg aagcggcgaa gtttaacgct agcgcaaatt aaaggcgcta
    17461 cgcgtaatgc gatggtgcga ccgcgttttg tcgcgccatt gccataagcg gaaagaccac
    17521 agtaacgcct ggtaaccgac cttcacgctg cgcacattgg taatcaggcg cttatacata
    17581 cccggaagta aatatctcgg caatcatccg ttggcgggta agaatatccg gctctttgcg
//
LOCUS       DAZSNM010000076        17341 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZSNM010000076 DAZSNM010000000
VERSION     DAZSNM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17341
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.076"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="ACHW42_002086"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="ACHW42_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR8943906.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="ACHW42_002087"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="ACHW42_002087"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR8943907.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /locus_tag="ACHW42_002088"
     CDS             2826..3980
                     /locus_tag="ACHW42_002088"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR8943908.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="ACHW42_002089"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="ACHW42_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR8943909.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="ACHW42_002090"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="ACHW42_002090"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR8943910.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="ACHW42_002091"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="ACHW42_002091"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR8943911.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="ACHW42_002092"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="ACHW42_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR8943912.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="ACHW42_002093"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="ACHW42_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR8943913.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="ACHW42_002094"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="ACHW42_002094"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR8943914.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="ACHW42_002095"
     CDS             11296..11511
                     /locus_tag="ACHW42_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR8943915.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACHW42_002096"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACHW42_002096"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR8943916.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="ACHW42_002097"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="ACHW42_002097"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR8943917.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="ACHW42_002098"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="ACHW42_002098"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR8943918.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="ACHW42_002099"
     CDS             complement(16247..16786)
                     /locus_tag="ACHW42_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR8943919.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="ACHW42_002100"
     CDS             complement(16802..17320)
                     /locus_tag="ACHW42_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR8943920.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT         4010 a         4315 c         4820 g         4196 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cttgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 a
//
LOCUS       DAZSNM010000077        17296 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZSNM010000077 DAZSNM010000000
VERSION     DAZSNM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17296
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.077"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            290..1429
                     /gene="arnB"
                     /locus_tag="ACHW42_002101"
     CDS             290..1429
                     /gene="arnB"
                     /locus_tag="ACHW42_002101"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR8943921.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1433..2401
                     /gene="arnC"
                     /locus_tag="ACHW42_002102"
     CDS             1433..2401
                     /gene="arnC"
                     /locus_tag="ACHW42_002102"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR8943922.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2401..4382
                     /gene="arnA"
                     /locus_tag="ACHW42_002103"
                     /pseudo
     CDS             2401..4382
                     /gene="arnA"
                     /locus_tag="ACHW42_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            4379..5269
                     /gene="arnD"
                     /locus_tag="ACHW42_002104"
     CDS             4379..5269
                     /gene="arnD"
                     /locus_tag="ACHW42_002104"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR8943923.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5269..6921
                     /gene="arnT"
                     /locus_tag="ACHW42_002105"
     CDS             5269..6921
                     /gene="arnT"
                     /locus_tag="ACHW42_002105"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR8943924.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            6918..7253
                     /gene="arnE"
                     /locus_tag="ACHW42_002106"
     CDS             6918..7253
                     /gene="arnE"
                     /locus_tag="ACHW42_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR8943925.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7253..7639
                     /gene="arnF"
                     /locus_tag="ACHW42_002107"
     CDS             7253..7639
                     /gene="arnF"
                     /locus_tag="ACHW42_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR8943926.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7633..7905)
                     /gene="pmrD"
                     /locus_tag="ACHW42_002108"
     CDS             complement(7633..7905)
                     /gene="pmrD"
                     /locus_tag="ACHW42_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR8943927.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(8010..9365)
                     /gene="menE"
                     /locus_tag="ACHW42_002109"
     CDS             complement(8010..9365)
                     /gene="menE"
                     /locus_tag="ACHW42_002109"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR8943928.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9362..10324)
                     /gene="menC"
                     /locus_tag="ACHW42_002110"
     CDS             complement(9362..10324)
                     /gene="menC"
                     /locus_tag="ACHW42_002110"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR8943929.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(10324..11181)
                     /gene="menB"
                     /locus_tag="ACHW42_002111"
     CDS             complement(10324..11181)
                     /gene="menB"
                     /locus_tag="ACHW42_002111"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR8943930.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11196..11954)
                     /gene="menH"
                     /locus_tag="ACHW42_002112"
     CDS             complement(11196..11954)
                     /gene="menH"
                     /locus_tag="ACHW42_002112"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR8943931.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11951..13621)
                     /gene="menD"
                     /locus_tag="ACHW42_002113"
     CDS             complement(11951..13621)
                     /gene="menD"
                     /locus_tag="ACHW42_002113"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR8943932.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13710..15005)
                     /gene="menF"
                     /locus_tag="ACHW42_002114"
     CDS             complement(13710..15005)
                     /gene="menF"
                     /locus_tag="ACHW42_002114"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR8943933.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(15084..15389)
                     /gene="elaB"
                     /locus_tag="ACHW42_002115"
     CDS             complement(15084..15389)
                     /gene="elaB"
                     /locus_tag="ACHW42_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR8943934.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15444..15905)
                     /locus_tag="ACHW42_002116"
     CDS             complement(15444..15905)
                     /locus_tag="ACHW42_002116"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8943935.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15970..16887
                     /gene="rbn"
                     /locus_tag="ACHW42_002117"
     CDS             15970..16887
                     /gene="rbn"
                     /locus_tag="ACHW42_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR8943936.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            17078..17266
                     /locus_tag="ACHW42_002118"
                     /pseudo
     CDS             17078..17266
                     /locus_tag="ACHW42_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         4062 a         4660 c         4374 g         4200 t
ORIGIN      
        1 caagcgcaac cagacaatta ccgtgaaatt gagcgacatt tctggcgata attagcagtt
       61 ggtgtaatat taaaaatcct acgatgtcgg caaaatgcct caaatttttg ccaaatgcaa
      121 agcctaaata agaaaaaata taaaaatttc aatatttacg tctaatatta gtttcttaag
      181 gttaagttaa tattctatcc ttaaaatttc gctccaaatg gcaaaatata cacaacactc
      241 tttatagcaa atataagtgg acaggtattc aatggcggaa ggaaaagcaa tgtcagaatt
      301 tttgcctttt tcgcgaccag caatgggcgt ggaggaactc gctgcagtta aagaggttct
      361 cgaatccggt tggatcacaa ccggtccgaa gaatcaggcg cttgagcaag ccttttgcca
      421 gttgacggga aatcagcatg ccatcgcggt cagttcagcc accgccggaa tgcatatcac
      481 gctaatggcg ttggaaattg gcaagggcga tgaagtgatt acgccttccc tgacctgggt
      541 ttcaaccctc aatatgattt ccttgctggg tgcaacgccg gtaatggtgg atgtcgaccg
      601 cgatacgctg atggtcacgc ctgaagcgat cgaatcagcc attacgccac gcactaaagc
      661 catcattccg gtgcattatg ccggtgcgcc agcagatatt gacgccattc gcgccattgg
      721 cgaacgttac ggcatcgcag ttatcgaaga tgctgcccat gccgtcggta cgtattacaa
      781 agggcgacat attggcgcaa aaggtaccgc tattttttca tttcatgcca ttaaaaatat
      841 tacctgtgct gaaggtggcc tgattgtaac tgataatgaa aaccttgccc gccagctacg
      901 gatgctgaaa tttcacggtc tgggtgtcga tgcctatgac agacaaacct ggggccgtgc
      961 accgcaggct gaagtcttaa caccgggcta taagtacaat ctgaccgata ttaacgccgc
     1021 gattgccctg acacagttag tcaaattaga gcacctcaac acccgtcggc gcgaaattgc
     1081 ccagcaatat cagcaagcac tggcagctct cccctttcag ccattaagcc ttcccgcctg
     1141 gccgcacgtt cacgcctggc atctgtttat tattcgtgtc gatgaacaac gttgtggtat
     1201 cagtcgcgat gcgttgatgg aagcgttaaa agaaagaggc attggtaccg ggttacattt
     1261 ccgcgccgct cacacacaaa aatattatcg cgagcgtttt cccacgctgt cgttaccgaa
     1321 taccgaatgg aatagcgaac gcatctgttc gttgccgctg ttcccggata tgactaccgc
     1381 cgatgccgac cgcgtcatca cagcccttca gcaactcgca ggacaataag ccatgtttga
     1441 aatccaccct gttaagaaag tctcggtggt tattcccgtt tataacgagc aggaaagctt
     1501 accggaatta atcaggcgca ccaccacagc ctgtgaatcg ttggggaaag agtatgagat
     1561 cctgctgatt gatgacggca gtagcgataa ttccgcgcat atgctggtcg aagcctcaca
     1621 agcggagaac agccatattg tgtctatttt gattaaccgc aattacgggc aacattcagc
     1681 gattatggcg ggattcagtc acgttactgg cgacttaatt attacccttg atgccgatct
     1741 ccagaatccg ccagaagaaa tcccccgcct ggtggcaaaa gccgatgaag gttacgacgt
     1801 ggtagggact gtacgccaga accgccagga cagctggttt cgtaaaacag cttcgaagat
     1861 gattaaccgg cttattcagc gcaccactgg caaagcgatg ggtgactacg gttgtatgct
     1921 gcgcgcctat cgccgtcata ttgtcgatgc gatgttgcac tgccatgaac gcagcacctt
     1981 tatcccgatt ctggcgaata tcttcgcccg ccgtgcaatt gaaattccag tacatcatgc
     2041 cgagcgtgag tttggtgaat ccaaatacag ttttatgcgc ctgattaatt tgatgtacga
     2101 cctggtgacc tgccttacca caacgccgct acgtatgctg agtctgctcg gcagcattat
     2161 tgcgattgga ggttttagca ttgcggtgct gctggtgatt ttacgcctga ccttcggacc
     2221 acaatgggcg gcagaaggcg tctttatgct atttgccgtg ctgtttactt ttattggcgc
     2281 tcagtttatc ggcatgggat tactcggtga atatatcggc aggatctaca ccgatgtccg
     2341 cgcccgcccc cgctattttg ttcagcaagt tatccgtcca tccagcaagg aaaatgaata
     2401 atgaaaaccg tcgtttttgc ctaccacgat atgggatgcc tcggtattga agctctgctg
     2461 gctgccggtt acgaaattag cgccattttt acccataccg ataatcccgg tgaaaaagcc
     2521 ttttatggtt cggtggctca tctggcggcg gaaagagaca ttccggttta tgcgccggat
     2581 aacgttaatc atccgctgtg ggtggaacgc attgcccaac tgtcaccaga ggtgattttc
     2641 tctttttatt atcgccatct tatttgcgac gagatttttc agctcgctcc cgcaggtgca
     2701 tttaatctgc acggttcgct gttaccaaaa tatcgtggtc gcgcgccgct gaactgggtg
     2761 ctggtgaacg gtgaaacgga aacgggcgtt acattgcacc gaatggtaaa cgtgccgatg
     2821 ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc gctattacgc
     2881 tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta cccgccatta
     2941 aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt tttggtcgca
     3001 gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg cacaacatgg
     3061 tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat cagaaattca
     3121 ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg agcgtgattt
     3181 ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc accggacagg
     3241 cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc ctggtgcaag
     3301 gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc
     3361 tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt
     3421 atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg aatcatccac
     3481 attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag tatcacgtca
     3541 aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa tatacccgca
     3601 acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc cgctactgcg
     3661 ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg atgtgtagcg
     3721 ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat aaaccacgct
     3781 ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat ggcgaaaaag
     3841 agggtttaca gttcaccctc ttcctcccgt ttaactggat gggaccacga ctggataacc
     3901 ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc aatctggtag
     3961 aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt actgatatcc
     4021 gggacggtat cgaggcgttg taccacatta tcgaaaatgc gggaaatcgc tgcgacggcg
     4081 aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg ggcgagatgc
     4141 tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt gcgggctttc
     4201 gcgttgtcga aagtagctgc tactacggca aaggatatca ggacgtagag catcgtaaac
     4261 cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat atgcaggaaa
     4321 ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat aaaccatcat
     4381 gaccaaagta ggcttacgca ttgatgtcga tgcctttcgt ggcacccgtg aaggcgtgcc
     4441 gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt tcagcgtcgg
     4501 cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt tgtggaagat
     4561 gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag gtacggcctg
     4621 gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca
     4681 cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg gtaactggga
     4741 tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag agattatcgg
     4801 tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc
     4861 aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatac cgttccgtcc
     4921 actgcttgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac ccacctggga
     4981 tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac tcaaccgcat
     5041 cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag gctgcgctta
     5101 tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga cattttgccc
     5161 tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg gaaatattgc
     5221 cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat gaaatcggta
     5281 cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc gatcagcacg
     5341 cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca
     5401 tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa acccattgcc
     5461 ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg
     5521 gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt tactctgcgc
     5581 ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc attgtttatt
     5641 gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg gctggtggcg
     5701 ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga
     5761 tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt tctcgccctt
     5821 gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc
     5881 tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc ctggggactg
     5941 gcgatagcgc agcgggagcc tgacttctgg cactattttt tctgggttga gcatattcaa
     6001 cgctttgcac tagatgatgc ccaacataga gctccgttct ggtactacgt gccggtcatc
     6061 attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac aggctggaaa
     6121 aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc gctgctgttt
     6181 ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc acctctggca
     6241 atgctgttgg cgcattacgc gttgctggca gcaaaaaata atcctctggc gctgcggatt
     6301 aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt tgtgatctcc
     6361 ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa agtcttttgt
     6421 gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac aaacgtcgaa
     6481 aagacctggt ctttcgccgc gctttgcccg ctggggctgg cgttgctggt aggattttca
     6541 attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa
     6601 tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc aggtctggca
     6661 tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt gaaatacggc
     6721 cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc aaactggctt
     6781 aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg cgatgaagat
     6841 atcaacagtc tcgccattcc gcccgcagat gccatcgttc gtcaggagcg tctggtgctg
     6901 attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg cttagcgttg
     6961 ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa cggcgcaaac
     7021 atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg atgctctggc
     7081 tgctggtctt gcagaacgta ccggtaggca ttgcctaccc gatgttaagt ctgaattttg
     7141 tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt
     7201 gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg taatgtgcct
     7261 gatatgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc tgggttttgc
     7321 ggcaagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc tgttagcttt
     7381 tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt ccgttttttg
     7441 ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc tcagtatgag
     7501 ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga ctttttcgct
     7561 gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt tcctgcccac
     7621 gacaaaacaa cgttactgag ttttccctgc cgctgtacac tgccgttccc actcatcggg
     7681 gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat taatacaata
     7741 aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt cactcttcac
     7801 ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat gtctgttatc
     7861 ttgtttattg cagcacgatt ttttgtgcca gccattccat tgcattatcc tgtttgctaa
     7921 gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg
     7981 cagaaatgag aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc
     8041 gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggtaaagt cagccagcgc
     8101 accggttgtt gaaaacgagc cagcttatct ttcacccatt cgccaagatc aacggtttgc
     8161 tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc aacggaaacg
     8221 ataaacacct gcaaaaccgc aggatgtgca gcaataacac gctcgacttc ttccggctga
     8281 ataccctcac cgccactgaa gaacagattg tctaaacgtc cgacaatggt cagcttgcca
     8341 ttatgcatct caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc
     8401 agttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta
     8461 ttaacgattt tcacttcccg acccggcagc ggcaaaccaa cgtctgccag gccgtcggct
     8521 tctttcgcac ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc
     8581 ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaacacc
     8641 gctttcaggg aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt
     8701 gaagcgtgag tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc
     8761 cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt
     8821 aaagagagca accagtcatc gtgatcgcca aacggaatca gcgataacac accttgcgca
     8881 ctggcaagat gggcctgata agtatggaca gcggctttcg gcaaaccggt agaacccgag
     8941 gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag cggcatgtac gccttcaacc
     9001 agctgaatgt gcagcgacgt taatgccgga aacgtgtttt ccccatccgg caccagcgca
     9061 aattgcagcg tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc
     9121 acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggcgagcag cgtttgcggc
     9181 gtattccacg cccgcaacat cacgccgctg ccctcaacca ccccctgcgc cgcaaagccc
     9241 gtagctaact catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga
     9301 cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag
     9361 atcataacaa ctgctcctgt acatcagcgt ccactaacgg cagtgggctc cccggccagc
     9421 gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg gtgtctggcg
     9481 ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca atggaagaac
     9541 tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt actttttcca
     9601 gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg
     9661 caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt tcacgggcaa
     9721 acgctcgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat
     9781 aatccgggtt aacatatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg
     9841 cgtcaagacg caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt
     9901 cgcgcaccgc ttcgtacaat cccactttga ccttcgccac tttctcgccc ggcatatcgg
     9961 caagtttgag gatcagatcg tccggatcgc cattacacag cggtgccgca cggtagttgg
    10021 ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca gcttacgcca aaggccacgg
    10081 aaggcatctg cggtagctcg caatcgcctg ccagccagtt atttacccag gcaagcagca
    10141 cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg cagtggggag atctcccccc
    10201 acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc
    10261 tgtcgcgcag aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac
    10321 gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt tgaaagcgtt
    10381 gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc
    10441 ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc agcgcagcgc
    10501 catcgggctg ttttgcagca tttcgcggca ccaacggacg gtttcttttt ccagatccgc
    10561 cagcggtacc acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg
    10621 gcacaggaac cagatttctc gcgctttttt ctgcccgacg atacgagcca tgtaggaagc
    10681 gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc
    10741 tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggagtagcc
    10801 agccaccatc gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac
    10861 gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg
    10921 gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgatatt
    10981 gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa
    11041 ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc
    11101 ataacgaatg tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg cgtaaggcat
    11161 tgcttcatca ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga
    11221 cttgcgatta cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca
    11281 tggcagtcgg cagccagttc cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat
    11341 aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg
    11401 agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcgcc
    11461 accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg
    11521 gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg atcggaacgc
    11581 tgacgttccg cacgttgttc agcattttgc agccccggat gcccgccttc gacaataacc
    11641 ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag
    11701 taccccacca gccagaagtc aaggatgttg taactaacca aggttttacg cagtaagtcg
    11761 gtgacatcat caaatccatc gacgctaatc gccgccgaac caccatgacc taggagatca
    11821 acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc gtggcaatcg
    11881 ccggaaaaac cgtgcaaaaa caccagccag ggtaaaccta gttttccgtg ttttgcctgc
    11941 gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt tgcgcaccat
    12001 cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag gcgtcggcaa
    12061 atgccgtttc aagttcctgc cagttttgcg gatgatgata tttcagctcg aacatcgcgg
    12121 cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcacgc tcgctttgcg
    12181 gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg
    12241 gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc gtaaagcgcg gagagatcgc
    12301 ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg gtcgaaagca
    12361 gtccgtcgat accgctggcc cctcggttgc tgtacaccgg gtaacctgcc ggaagttgcg
    12421 aaagcgcatc aatcagacgt accaccaggc tgttaccaac aaacaattgc ccctgttcag
    12481 gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca cggcgggcaa
    12541 taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt
    12601 tttctgccgg atgcagctcc agccagtcgg caatattggc aattaagcga cgtccgcgat
    12661 ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct tctggttcac
    12721 agcttgcctg ccattgcagg agccgtttcc ccgtcaggct acttcccagt tgcaccacaa
    12781 tttgcgcctg ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga tcggcacacg
    12841 gcagcggctg cccggtttgt gacagcacat cgccaatcag cggccagcca agagtttgcg
    12901 cccacagggc aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc
    12961 gcttttgtcg ccagaagaac cagtcgcgct gtttttcact ttccagacga ggcgcttcac
    13021 gcagccacgg tttgtcgtcc tgccaccagt cacccagacg ctgttgccag ctaagcccgg
    13081 tatcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg actccccccg
    13141 catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct
    13201 gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt cccggctggc
    13261 gaattgcctg attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaagagaa
    13321 tcagtttttc tccggttaac ccggcttcaa tcagtgccgg atagagattt gccaccgccg
    13381 tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca
    13441 gatgccccaa cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg
    13501 ccgctaacgt taacggtgta gaacgcgagc ctggggcgat acagatgtgt ctgacgccgt
    13561 gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca
    13621 tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg
    13681 atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct
    13741 gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgccc
    13801 gcgccagcat ataatcgcac gacattgccg ctaatttttg ctgagcgcag ggaaacgcag
    13861 aattcgcttt gttgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac
    13921 ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc
    13981 gttggcagca actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata
    14041 cagcggcgaa gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc
    14101 gtctgggtat cggcctgtaa tcgttggcag atatcttcca ccaccagcat gttctcgcgc
    14161 tggtttttat catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga
    14221 tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat
    14281 aaccgttccg gtgaagagcc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg
    14341 tagcaattca gattcagtcg acgactggca gccatcatcg ccgccgcgtt gaccggactt
    14401 gcgaaatgca ggtcagttgc ccgagcgagc accactttat cgagctcacc ttcggcgatg
    14461 gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct
    14521 cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat
    14581 tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc
    14641 gtggctttac cgccacagcg tcgccattcc aggcggggta aaagtaaatt gccctgcgac
    14701 gggtcaaaag cattcagccc ccaaatgcgt aagtcagcgt gttccgggtg ctggcgaaga
    14761 aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct
    14821 tcatcaccat tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag
    14881 ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt
    14941 gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat
    15001 tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg
    15061 cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagcccg
    15121 acggccgcgc ccacaccaat tccttgccag ggtttttcgt ggacgtagtc atcagcacga
    15181 taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc
    15241 acatcatcca gcgctttttc tgcatgcgct ttcagctcaa catatttctg atcggcggga
    15301 tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg
    15361 atacgtgtat caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac
    15421 taactataga caagagttgt tgattacgcc tgaattacct cgcgtgccat cccaatgtgt
    15481 gggataccat cttcttcata gacctccgtc acagggataa aaccgaaact ctggtagaag
    15541 ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat caggccagtg atgcgtacat
    15601 gtttcgagtg ttttactcat cagctgctgc cccacttttt caccacgcaa cgcttcactg
    15661 acaatcaccc gacctataac gaccggctca agatcatcat cacttttcag aatcctcgca
    15721 tacgccacca gttcatcatt tttccaaccg agaatatggc gattatcccc cgtcaaatca
    15781 tcaccatcga tatcctgata agggcaattc tgctcgacaa caaacaccgc gcaacgcagt
    15841 tgtaataagg cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca
    15901 atcataatgc tctcctttta gatgtatgcc ttatactagc cggaatattt tttgaaacgt
    15961 gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac ccgcacgcgc
    16021 aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact ttggttgttt
    16081 gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg
    16141 gacaagattt ttatcagtca ccttcatggc gatcatcttt ttggtttacc cggcttgctg
    16201 tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc gcaaggtatc
    16261 cgtgaatttg tggaaaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa
    16321 attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt aaccgcttat
    16381 ccgctggaac acccactgga atgttatggc tatcgtattg aagaacatga taaaccgggt
    16441 gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc ctggcccact gtttcaggaa
    16501 ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc agattaacgg cgcagattac
    16561 ttagctgctc cagtgccagg taaagcgctc gctattttcg gcgataccgg cccctgcgat
    16621 gccgcacttg acctggctaa aggtgtcgat gtcatggtgc acgaagcgac gctggatata
    16681 accatggaag ccaaagccaa tagtcgcggc catagctcta cacgccaggc tgcgacacta
    16741 gcccgtgagg ctggagtcgg caagctaatc attacccacg tcagctcgcg ctatgatgac
    16801 aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt tcccggcgac tgaactggcg
    16861 aatgatttca ccgtgtttaa cgtttaactc tgcttattat taaaaacagg gtgaaacttg
    16921 ccctgttata gcaacccgcg ctacatatat ccacccacac aggcatattg agcgattttt
    16981 gatatttatt catctcctta cgcactatat tcagagcaat aagttgcctc ttttcatctt
    17041 gattcagcac aacctttgca actaaaagga gcccatgatg gttacagttg tcagcaatta
    17101 ttgtcaatta tctcaaaccc aactcagtca gacatttgca gaaaaattta ctgtgaccga
    17161 ggaattactg cagtctttaa aaaaaacagc gttatccgga gatgaagaaa gtattgagtt
    17221 actgcataat attgcgttag gttatgatga atttggaaaa aaagctgggt aatgctgcca
    17281 acttactgat ttagtg
//
LOCUS       DAZSNM010000078        17224 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZSNM010000078 DAZSNM010000000
VERSION     DAZSNM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17224
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.078"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..729)
                     /locus_tag="ACHW42_002119"
     CDS             complement(<1..729)
                     /locus_tag="ACHW42_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR8943937.1"
                     /translation="MKTFSNSWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHL"
     gene            complement(726..1497)
                     /gene="thiG"
                     /locus_tag="ACHW42_002120"
                     /pseudo
     CDS             complement(726..1497)
                     /gene="thiG"
                     /locus_tag="ACHW42_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
     gene            complement(1499..1699)
                     /gene="thiS"
                     /locus_tag="ACHW42_002121"
     CDS             complement(1499..1699)
                     /gene="thiS"
                     /locus_tag="ACHW42_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR8943938.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(1683..2438)
                     /gene="thiF"
                     /locus_tag="ACHW42_002122"
     CDS             complement(1683..2438)
                     /gene="thiF"
                     /locus_tag="ACHW42_002122"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR8943939.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(2431..3066)
                     /gene="thiE"
                     /locus_tag="ACHW42_002123"
     CDS             complement(2431..3066)
                     /gene="thiE"
                     /locus_tag="ACHW42_002123"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR8943940.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(3066..4961)
                     /gene="thiC"
                     /locus_tag="ACHW42_002124"
     CDS             complement(3066..4961)
                     /gene="thiC"
                     /locus_tag="ACHW42_002124"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR8943941.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(5042..5142)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5195..5671)
                     /gene="rsd"
                     /locus_tag="ACHW42_002125"
     CDS             complement(5195..5671)
                     /gene="rsd"
                     /locus_tag="ACHW42_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFR8943942.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            5766..6539
                     /gene="nudC"
                     /locus_tag="ACHW42_002126"
     CDS             5766..6539
                     /gene="nudC"
                     /locus_tag="ACHW42_002126"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR8943943.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            6579..7643
                     /gene="hemE"
                     /locus_tag="ACHW42_002127"
     CDS             6579..7643
                     /gene="hemE"
                     /locus_tag="ACHW42_002127"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR8943944.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            7800..8471
                     /gene="nfi"
                     /locus_tag="ACHW42_002128"
     CDS             7800..8471
                     /gene="nfi"
                     /locus_tag="ACHW42_002128"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR8943945.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            8514..9104
                     /locus_tag="ACHW42_002129"
     CDS             8514..9104
                     /locus_tag="ACHW42_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR8943946.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            9291..9563
                     /gene="hupA"
                     /locus_tag="ACHW42_002130"
     CDS             9291..9563
                     /gene="hupA"
                     /locus_tag="ACHW42_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR8943947.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            9576..10271
                     /locus_tag="ACHW42_002131"
     CDS             9576..10271
                     /locus_tag="ACHW42_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR8943948.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(10273..10698)
                     /gene="zraP"
                     /locus_tag="ACHW42_002132"
     CDS             complement(10273..10698)
                     /gene="zraP"
                     /locus_tag="ACHW42_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR8943949.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            10936..12312
                     /gene="zraS"
                     /locus_tag="ACHW42_002133"
     CDS             10936..12312
                     /gene="zraS"
                     /locus_tag="ACHW42_002133"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR8943950.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            12309..13634
                     /gene="zraR"
                     /locus_tag="ACHW42_002134"
     CDS             12309..13634
                     /gene="zraR"
                     /locus_tag="ACHW42_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR8943951.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(13631..14920)
                     /gene="purD"
                     /locus_tag="ACHW42_002135"
     CDS             complement(13631..14920)
                     /gene="purD"
                     /locus_tag="ACHW42_002135"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR8943952.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(14932..16521)
                     /gene="purH"
                     /locus_tag="ACHW42_002136"
     CDS             complement(14932..16521)
                     /gene="purH"
                     /locus_tag="ACHW42_002136"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR8943953.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            17136..>17224
                     /locus_tag="ACHW42_002137"
     rRNA            17136..>17224
                     /locus_tag="ACHW42_002137"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         3874 a         4631 c         4753 g         3966 t
ORIGIN      
        1 caaatgttcg gcaaccatat agcagtcaac gcgccagttg tcggaaaggc caattagcgc
       61 gccgaggcct atcttatcaa tccccgcacg ccccagccga tccggcgttt ccagccgcca
      121 gaagaagtcc tgttttttgc ctttcaaatg atggcgggta taagtcgcct cgtgatatgt
      181 ctcctgataa accatcaccc catccagacc aagttgcttt aactcggcgt attccgtctc
      241 cgccagcggt tgcacttcca tttgtagtga agagaactgt tcacgcaggg cggggagatg
      301 acgacgaaag taatccatcc ccactttcgc ctgatgttca ccagtgacta atagcagatg
      361 ttcaaagccc atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt
      421 tttgcgcttg atgcgattac tcatggaaaa tccgcagtac gtgcagtcgt tagcgcaaag
      481 attggaaaga taaagcggga cgtagaaact aactgtgttg ccaaatcgct gacgggtcag
      541 acgctgcgcc tgttgggcca gttgttccag atagccactg gcggcaggcg ataacagcgc
      601 catcatgtcg tcgcgggtga gttgcgaggc atttagcgcc cgctctacgt cagcagccgt
      661 tttgccgttg atacgcaggc ggatgtcgtc ccagtccagt tgtcgccagc tattgctgaa
      721 ggttttcatg ccgatgcctc cagaaatccg gtcagcgggc tggtggcatg agcaaaataa
      781 ctgcggctgc ccggtccgga ctgacgtgcc agcaggccta cttctaccgc cagacgaaat
      841 gccttcgcca tgttgacggg atcgtccgcg acggcaatcg ccgtattcac taacaccgcg
      901 tcggccccca tttccagcgc ctgcgtggca tggctgggaa cgccgatgcc agcatcgaca
      961 accaccggaa ctgtggcctg ctggataata atctccagca tggcacgggt ttccagtccc
     1021 tgattcgagc caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga
     1081 cgtttacaca ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc
     1141 agcgtttcgg cggctttcag ggtttcgatg ggatcgggca acagccagcg ggcgtcaggg
     1201 tgaatctcca attttagcca gtttgtgcct aacgcttcac gtgccagatg ggcggcgaaa
     1261 atggcttctt ccgctgtttt cgccccggaa gtattcggca gcagggccac acccgccgcc
     1321 gataagcggt tcgaggatag cgtcgttgtg ctggcgcaag tcgacacgtt tcatcgccag
     1381 tgtcaccagc tggctgccgg aagcgcggat cgcctccacc atcagttgtg aagaagcgaa
     1441 tttccctgtg ccggtaaaca gatgtgaatc aaacgttttg tccgcaatac gtaacatctc
     1501 aaccccctgc aataacctga aaaagcagga tctggtcgcc atcctgcacg atatgttgcg
     1561 tccactgctc acgcgggacg atttgctgat taatcgccag agccgcgccc gcttgtcgtt
     1621 ggtcgagttg ctccagtagt tcgtgaacag tttgcccggc ggcgcactgc atcggttgat
     1681 cgttaaacag gatctgcatt gcttcctccg cataccgggc aaccactggc gcggcgcaac
     1741 gccaggctgc gccactggct cgacttaccg tcgaacagtc ggagttctcc cgcaggcgtc
     1801 tctataccgc ttaataactt aatggcttcc agtgcctgca aagtgcccat caccccgacc
     1861 accgggccaa ccacgcccgc cgtgcggcag ttgcgttctg gctgctggtt atctggccac
     1921 aggcagcggt aacacccctg ctcccagggc ggtgtcagta ccatcaactg accgccaaat
     1981 ccgaccgcgc tggcggtgat aagcggcgtg ttgagtgcca cgcaggcggc attaatctcc
     2041 tggcgagtcg ccatattgtc ggtacagtcg agcaccacat cggcttgtgc aaccgcatct
     2101 ttcaacgcct cacccgttaa ccgttgttgt aatgctgtca gttgaatgtc gggattcaac
     2161 tgtgtcagtc gctgttggct gacctgcgat ttcgggctgt tgatatcttc agtggtaaag
     2221 aggatttgtc gttgcagatt gcttaaatgc acatcgtcgt catctgccag taccagcgtc
     2281 ccgacgccag cccctgccag gtatagcgca gcaggtgtac ccagcccgcc aagaccaata
     2341 atcagcacct ggctgtcgag cagtttttgc tgcccgtcaa gagcgatatc gtcgagcaga
     2401 atttggcggc tataacgcat aaagtcacgg tcattcatcg ccaactcctg caatttccag
     2461 caactgtgcc gttgccaaac gccagtctgc ggcttgagta atggcgctga cgacggcgat
     2521 actgccgaca cccgttgcta tcaccgcagg cgcgcgtgcc agactgatac cgccaatcgc
     2581 cacggtggga taatccgcca ggcgctcaac atgccgtgcc aactgttcca gcccctgcgg
     2641 tgcagaaggc atctgtttgg tttgcgtcgg gaacacatgt cccagcgcga tataagaggg
     2701 gcgtgctgcc agcgcgacgt cgatttccat atcgtcatgt gtcgaaacgc ccagccgcag
     2761 gcctgccgcg cggatggcat tgagatcggt ggcttgcaaa tcttcctgcc ccaaatggac
     2821 gccatacgcc tgatgcttga tcgcgagccg ccagtaatcg ttgataaaca atcgcgcgtt
     2881 atagcggcgg cccagcgcaa ttgccgccac gacatcggct tccacctctt catcgcgccg
     2941 atctttgatg cgtagctgga gagtacgtac gcctgcatcc aacagacgtt cgatccactg
     3001 tacgctgtcc accaccgggt acagtcctaa acgaaaaggt acaggaggaa aatcaggctg
     3061 atacatcacg cttcctcctt atgcaggtag atttctccgc ctctggcacg gaagttctcc
     3121 gacatatccg ccattcccac ttcaatggtt tgcgcggcgg cgtaatcgcg tacttcctgg
     3181 ctgattttca tcgagcagaa ttttggccca cacatggagc aaaaatgggc gactttgccg
     3241 gactcttggg gcagggtttc atcgtgataa gcgcgggcgg taaacgggtc gagggccaga
     3301 ttaaactggt cttcccagcg aaattcgaag cgggctttcg acatggcgtt atcgcgaatt
     3361 tgcgcgcccg gatgcccttt tgccaggtca gcagcgtggg cggcaatctt ataagtgata
     3421 agcccctgct taacatcttc tttattgggc agacccagat gctcttttgg cgttacgtaa
     3481 cagagcatcg cgcagccaaa ccagccaatc atcgccgcac caatccccga cgtgaagtgg
     3541 tcatagcccg gtgcaatatc ggtagttagc ggccccagag tgtaaaacgg cgcttcgtgg
     3601 cagtgctcta actcctcggt catattgcgg cggatcatct gcatcggcac atggcctggg
     3661 ccttcaatca tcacctgtac gtcatattcc caggcgattt tggtcagttc gcccagcgta
     3721 tgcaattcgg caaactgcgc ttcatcgttg gcgtcctgaa tagaaccggg gcgcagaccg
     3781 tcgcccagcg acagcgaaac gtcataagcg gcacagattt cacaaatttc gcggaagtgt
     3841 tgatagagga aattttcctg atgatgggag aggcaccatt tcgccataat cgaaccgccg
     3901 cgagagacga taccggtcag gcgtttcgcg gtcatcggca catagcgcag cagtacgccc
     3961 gcatggatag tgaagtaatc cacaccttgc tcggcctgtt ccagcagcgt gtcgcggaac
     4021 gcttcccagg taagatcttc ggcgatcccg ttaaccttct ccagcgcctg gtagatcggc
     4081 actgtaccga tcggcaccgg actgttacgc aaaatccact cgcgggtttc gtgaatatag
     4141 cgaccggtgg agagatccat caccgtatcc gctccccagc gcgtggacca taccagcttt
     4201 tccacttctt cttcgatgga agaggtgacc gccgagttgc cgatattggc gttaactttt
     4261 accaggaagt tacggccaat aatcatcggc tccgattccg gatgattaat gttggccggg
     4321 ataatcgcac gtccggcagc aacttcatca cggacaaatt ccgcagtgat attttccggc
     4381 agatgtgcgc caaagctcat tcccggatgc tggtggcgta aaacctcgct acggatgcgc
     4441 tcgcggccca tattctcgcg gatggcgatg aattccattt ccggcgtgat gatgccctgg
     4501 cgggcgtagt gcagttgggt gacacggcgt cctgttttgg cgcgttttgg cgttaataag
     4561 ccgctaaaac gtagctcgtc gagaccatca tctgccagcc gcgttctggt gtaatcggaa
     4621 ctgcgcacgg taagttcttc ggtatcggcg cgcgcatcga tccacggctg gcgtagtttt
     4681 gccagccctt gctgcacgtt aatggcgatc tgcggatcac catacgggcc ggaggtgtcg
     4741 tagaccggaa tcgcttcgtt ttcttcgtac tgcggctgtt ctttgctacc gccaattagc
     4801 gtcgggctaa gctggatctc acgcatcggc acgcgcacgc cggggtgtgt gccagtgata
     4861 taaatgcgtt ttgagttggg aaaggcggtg ccttccaggg tgtcgataaa atgttgggcc
     4921 cgggcgcgtt gttcgcggcg ggtcagtttt gttgcagaca tagctcattc caaaaagtta
     4981 aggacgtggc ttgtcagacg acggatgaag caagagacga tcgccgcaaa ggcgatgcga
     5041 tagcagatta actcttgttc ccttcgcagg tattagcctg atcaggttcc gcggatcccg
     5101 aataaacggt ctcagccagt taaggcactc cgacaagaaa ttagccccca caaacggggc
     5161 gttgaatgta aattacgcgt taacagcgca gaactcaagc aggatgtttg acgcgggcgg
     5221 cgtcaagcac cagcagaatc agcttatctt ccagcacaaa gcgcgcttcc agcgcctcgc
     5281 cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc atgatcgatg gcggtttcca
     5341 ggctggaatc gtagtaatcc ataatctgtt gggtattggc ttcgagttgc ggccaaatct
     5401 tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg aagaatgcgc tcataaatac
     5461 tgaaatgtcc ggcagacaag taatcgacca ggctctgaca aaaatcatca agggcttttt
     5521 cgtttagcct catgtacgat tctttgccag gcttaatgcc aaccagattg tagtaagcca
     5581 cgagcagatg cttacgtaca tgtagccagc gatcaaccag tttgttactt cctctgacgc
     5641 gctccgtcag gttatcgagc tggttaagca tgattgactc cgcaagtttg tattcaaaaa
     5701 ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca gtgatgcaaa ggtagtgcaa
     5761 gagctatgga tcgtataatt gaaaaattag atcacggctg gtgggtcgtc agccatgaac
     5821 aaaaattatg gttgccgaag ggagaattgc catatggcga agcggcaaat ttcgatcttg
     5881 tgggtcagcg cgcactacaa atcggcgaat ggcaggggga acctgtttgg ttaatacaac
     5941 agcagcggcg tcacgatatg gggtcggtac gtcaggtcat tgatctcgat gttgggctgt
     6001 ttcaactggc cggacgtggc gtgcaactgg cggagtttta ccgatcgcat aaatactgtg
     6061 gttactgcgg gcatgaaatg tatccgagca aaaccgaatg ggcgatgctg tgtagccatt
     6121 gccgtgagcg ttactacccg caaatcgccc cctgcattat tgttgccatc cgtcgcgatg
     6181 attcgctcct cctcgcccag catacccgcc atcgtaacgg tgtccataca gtacttgccg
     6241 gattcgtcga agtgggcgaa accctcgagc aggcagtcgc gcgggaagtg atggaagaga
     6301 gcggaattaa agttaaaaac ttgcgttacg tgacttctca gccgtggccg tttcctcagt
     6361 ctttaatgac cgcgtttatg gcggaatatg acagcggcga catcgtgatc gacccgaaag
     6421 aattgctcga ggcgaactgg tatcgctatg acgatttgcc gttactcccg ccgcccggca
     6481 ccgtagcgcg ccgtctgata gaagatactg tggcgatgtg tcgggcagag tatgagtgat
     6541 gatacactga ccgcctgacg cactaaggaa cagccaaaat gaccgaactt aaaaacgatc
     6601 gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac tccagtatgg atgatgcgcc
     6661 aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc ccaggcgggc gattttatgt
     6721 cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt gcaaccgctg cgtcgctacc
     6781 cgctggatgc ggcgatcctc ttttccgata tcctcaccgt gccggacgcg atggggttag
     6841 ggctctattt tgaagccgga gaaggtccgc gttttacctc gccagtcacc tgcaaagccg
     6901 acgtcgataa actgccaatt ccggacccgg aagatgagct gggttacgtg atgaacgcgg
     6961 tgcgtaccat tcgtcacgaa ctgaaaggcg aagtgccgct gattggtttt tccggcagcc
     7021 cgtggacgct ggcaacctac atggtggaag gcggcagcag caaagccttc accgtgatca
     7081 aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct gctcgataaa ctggcgaaaa
     7141 gcgtcacttt gtatctgaat gcgcagatta aagccggtgc tcaggcagtg atgattttcg
     7201 acacctgggg cggcgtgctt accgggcgcg attatcaaca gttctcgctc tattacatgc
     7261 ataaaattgt tgatggttta ctgcgtgaaa acgacggtcg ccgcgtgcca gtcacgctgt
     7321 ttaccaaagg cggcggacag tggctggaag ccatggctga aaccggttgc gatgcgctgg
     7381 gcctcgactg gacaacggac atcgccgatg cgcgccgccg tgtgggcaat aaagtcgcgt
     7441 tgcagggtaa tatggatccg tcgatgctgt acgcgccgcc tgcccgcatt gaagaagaag
     7501 tagcgactat acttgcaggt ttcggtcacg gcgaaggtca tgtctttaac cttggtcacg
     7561 gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt cgtggaggca gtgcatcgac
     7621 tgtctgaaca gtatcaccgc taagagcctg atccatcagg ctattttatt tgccattttg
     7681 aggccgggca gtgctcaaaa tcctcacgta ctacgtgtac gctccggttt ttccgcgctg
     7741 tccggcctca aactgccttc aacaattacg cctgttgaac caggctctaa ggagcgatta
     7801 tggatctcgc gtcattacgc gctcaacaaa tcgaactggc ttcttctgtg atctgcgagg
     7861 atcgactcga taaagatcca ccggatctga tcgccggagc cgatgtcggg tttgagcagg
     7921 gcggagaagt gacgcgagcg gcgatggtgc tgctgaaata tccctcgctt gagctggtgg
     7981 agtataaagt tgcccgcatc gccaccacca tgccttacat tccaggtttt ctttccttcc
     8041 gcgaatatcc tgcgctgctg gcagcgtggg agatgctgtc gcaaaagccg gatttagtgt
     8101 ttgtcgatgg tcatgggatc tcgcatcctc gccgtcttgg cgtcgccagc cattttggct
     8161 tattggtgga tgtgccgacc attggcgtgg cgaaaaaacg gctctgcggt aaattcgaac
     8221 cgctctccag caaaccgggc gcgctggccc cactgatgga taaaggcgag cagctggcct
     8281 gggtctggcg cagcaaagcg cgctgtaacc cgttgtttat cgctaccggc catcgggtca
     8341 gcgtggacag cgcgctggcg tgggtacaac gctgcatgaa aggctatcgt ctgccggagc
     8401 caacgcgctg ggcggacgcg gtggcctcgg aacgtccggc gttcgtgcgc tatacagcaa
     8461 atcagcccta attcaggtaa actgcggata atttccgtat ttgagaactc aacatgttac
     8521 aaaacccaat tcatctgcgt ctggagcgcc tggaaagctg gcagcatgtc actttcatgg
     8581 cttgcttatg cgaacgcatg taccccaatt acgccatgtt ctgccagcaa accggttttg
     8641 gtgatgggca aatttaccgt cgtattctcg atctcatctg ggaaacgctg accgttaaag
     8701 acgcaaaagt aaatttcgac agccaactgg agaaatttga agaagcgatt ccttcagccg
     8761 acgatttcga tctgtacggc gtttatccgg caatcgatgc ctgcgtggcg ttaagtgaac
     8821 tggtccattc gcgtttgagt ggtgaaacgc tcgaacacgc ggtggaagtg agtaagacct
     8881 ccatcacgac cgttgcgatg ctggaaatga ctcaggctgg tcgcgaaatg ggcgatgaag
     8941 agcttaaaga aaacccagct gtagagcaag aatgggacat tcagtgggaa atattccgac
     9001 ttttagctga gtgcgaagaa cgcgatatcg agctgataaa aggccttagg gcagacctgc
     9061 gtgagtcggg tgagagcaat attggtataa tttttcagca ataagaccag aaaacgtgat
     9121 ttaacgcctg atttgtcgta cctggagtct tccctttcgc cccccgtctg gtctacattt
     9181 ggggggcgaa aaaaagtggc tatcggtgcg tgtatgcagg agagtgctat tctggcattt
     9241 ccgtcgcact cgatgcttag caagcgataa acacattgta aggataactt atgaacaaga
     9301 ctcaactgat tgatgtaatt gcagagaaag cagaactgtc caaaacccag gctaaagctg
     9361 ctctggagtc cactctggct gcaattactg agtctctgaa agaaggcgat gctgtacaac
     9421 tggttggttt cggtaccttc aaagtgaacc atcgcgctga gcgtactggc cgcaacccgc
     9481 agaccggtaa agaaatcaaa attgccgcag ctaacgtacc ggcatttgtt tctggcaagg
     9541 cactgaaaga cgcagttaag taagattgcg tggcagtgaa cagttttaac gaaggggtgg
     9601 tttcacccct tttgtctttc tggcgtcgat cattgatgct ggcaggtgcg ctgtttctca
     9661 ctgcctgtag ccacaactct tcacttcctc cctttaccgc cagtggattt gctgaagacc
     9721 agggcgcggt acgcatctgg cgaaaagaca gcggcgataa tgtgcatctg cttgccgtgt
     9781 ttagcccgtg gcgcagtggc gataccacga cgcgagagta tcgctggcag ggcgataacc
     9841 tcacgctcat caatatcaat gtttacagca aaccgccggt gaacattcgc gcgcgttttg
     9901 acgatcgcgg cgatctgagc tttatgcaac gtgaatccga tggggaaaag cagcagcttt
     9961 ctaacgacca aatcgattta taccgttatc gtgctgatca gatccgccag attagcgatg
    10021 ccttacgtca ggggagagtc gtgctgcgcc aggggcgctg gcatgcgatg gaacagaccg
    10081 tgaccacctg cgaagggcaa accattaaac ctgatttaga ttcgcaggcg atagcgcata
    10141 tcgagcgccg ccagagccgc tcttctgttg atgtcagcgt ggcatggctg gaagcgcccg
    10201 aaggttcgca attactgtta gtggcaaact ctgatttctg tcgctggcaa cccaacgaga
    10261 aaacgttctg atttaccagt ggcccatacc catatgaccg ccaccaccgc agccgccgta
    10321 gcccatgccc attccggctc cgcgcggaat acccgcttca gccatcgcga tatctcgttt
    10381 cacccgtaac tcatctaacg actgacgcaa attctccatc tctttggcga ccgcgttaat
    10441 tttgctgcta tccggtgggt tcgcggctaa cagggcattg tattcataac gcttcgtcac
    10501 cagttgctgt tgcagtgcgc tgctttgagc gtaaaagtca ttatggattt tctgccacgc
    10561 tgtctgttgt tcgctggtca aaggcgcggc attttgctgc cacataccgt gtccgccgtg
    10621 agcaaatgca gatgtcgatc ccatcgccat tgctgaaagc gccatcatta ccagggcaat
    10681 tttcgtgttc cgtttcatgg ttaatcctcc agtggttgtc ttacttcggg tattgcatct
    10741 tccgtgccaa cgatgaaacg ctgatatgac gggtaatctg gcatgataaa cgagtaaaaa
    10801 tgactcgcct gctgcgggta gcgagtcatt tttactcatt gaaacttaag cctttgtgtt
    10861 acagcgcagg gtaagcgctg ataaaagatg gcatgatttc tgctgtcaga aagggatgag
    10921 caggcaaaga agaagatgcg ttttatgcaa cgttctaaag actccttagc taaatggtta
    10981 agcgcgatcc tccccgtggt cattgttggg ctggtagggt tgtttgcggt gacggtgatt
    11041 cgcgattatg ggcgcgagac tgccgccgcc agacaaacgc tgctggaaaa aggcagtgta
    11101 cttattcgcg ctcttgaatc cggctcgcgc gtcggcatgg ggatgcgcat gcatcatgcg
    11161 cagcagcagg ccttactgga agaaatggcc gggcagcctg gagtacgttg gtttgcggtc
    11221 acggatgaac aaggaacaat cgtgatgcat agcaactccg gcatggtggg aaaacagctt
    11281 tattcccctc aggaaatgca gcagttacat ccgggagatg aagaagcgtg gcggcggatc
    11341 gatagcgcag acggcgagcc tgttctggaa atttatcgcc agtttcaacc gatgtttgct
    11401 gctggaatgt accggatgcg ccatatgcag cagtatgccg cgacaccaca agcaattttc
    11461 atcgctttcg acgccagtaa tattgtgagt gccgaagatc gtgagcagag aaacaccctg
    11521 attattctct tcgccctggc gacggtcttg ctggcaagcg tattgtcatt cttctggtat
    11581 cgccgctatc tgcgctcgcg ccagcttcta caagatgaaa tgaagcgcaa agagaagctg
    11641 gtggcgctgg ggcatcttgc ggcaggcgtt gcccacgaaa tccgtaaccc actttcctcg
    11701 attaaaggac tggcgaaata ctttgccgag cgcgcgcctg cagggggaga agcgcatcaa
    11761 ctggcgcagg tgatggcgaa agaggccgac cgtttaaacc gcgtggtaag cgagttgctg
    11821 gaactggtta agccaacgca tctggctttg caggcggtgg atctcaacac gctgattaac
    11881 cactcattac agctggtaag ccaggatgca aacagccggg agatccagtt acgctttacc
    11941 gccaacgaca cattaccgga aattcaggcc gatccggaca ggctgactca ggtcctgttg
    12001 aatctctatc tcaatgctat tcaggcgatt ggtcagcatg gcgtgattag cgtgacggcc
    12061 agcgaaagcg gcgcgggcgt gaaaatcagc gttaccgaca gcggtaaggg aattgcggca
    12121 gatcagcttg aagccatctt cactccgtac ttcaccacca aagccgaagg caccggattg
    12181 gggctggcgg tcgtgcataa tattgttgaa caacacggtg gtacaattca ggtcgcaagc
    12241 caggagggaa aaggctcaac gttcaccctc tggcttccgg tcaatattac gcgtaaggac
    12301 ccacaaggat gacgcacgat aatatcgata ttctggtggt ggatgatgac attagccact
    12361 gcactatttt gcaggcttta ctgcgcggct ggggctataa cgtcgcgctg gcgaacagcg
    12421 ggcgacaggc gctggagcag gtgcgggaac aggtttttga tcttgtgctt tgcgatgtgc
    12481 gaatggcgga gatggacggc atcgccacgc tgaaagagat caaagcgtta aacccggcaa
    12541 ttccggtgct gattatgact gcgtactcca gcgtcgagac ggcggtagag gcactgaaaa
    12601 ctggggcgca ggattatctc atcaagccgc tggatttcga taacctacag gcgacgctgg
    12661 aaaaggcgct cgcgcatacg cacagtattg atgctgaaac acctgcggtg actgccagcc
    12721 agttcggtat ggtcggtaaa agcccggcga tgcaacacct gctcagtgaa atcgccctcg
    12781 tcgcgccatc ggaagccacg gtgctgatcc acggcgattc cggcaccggt aaagagctgg
    12841 tcgccagggc gattcacgcc agtagcgcac gtagcgaaaa accgctggta acgctcaact
    12901 gtgcggcact caacgaatcc ttgctggaat ctgaattgtt cggtcacgaa aaaggggcgt
    12961 ttactggagc cgataaacgc cgggaagggc gctttgttga ggcggacggc ggcacgctgt
    13021 ttctcgatga aattggcgat atctcgccga tgatgcaggt gcgtctgctg cgtgcgattc
    13081 aggagcgcga agttcagcgt gtcggtagca accagactat ctcggttgat gtccggctga
    13141 ttgcggcgac ccatcgcgat cttgccgcag aggtgaatgc cgggcgtttt cgccaggatc
    13201 tctactatcg cctgaatgtg gtggcgattg aagtaccatc gctgcggcaa cggcgggaag
    13261 atatccctct gctggctggc cattttcttc agcgctttgc cgagcgtaat cgcaaggcgg
    13321 taaaaggctt tacgccacag gcgatggatt tgctgatcca ctacgactgg ccgggaaata
    13381 ttcgtgaact ggaaaacgcg gtggaacggg cggtggtgct gctgaccggg gaatatattt
    13441 ccgaacgcga gctgccgctg gcgattgcca gtacgccgat cccgctgggg caaagtcagg
    13501 atattcagcc gctggtggaa gtggaaaaag aggtgattct ggcggcgctg gagaaaacgg
    13561 gcggcaacaa aaccgaagcc gcccgtcagt tagggatcac gcgcaaaacg ctattggcaa
    13621 aactgtcgcg ttagttctgc tcgcgttcga tggcgcgcca accgatatct ttccggcaga
    13681 agcaatcatc ccagtggata tcggtcatta aggcgtaggc gcgtttctgc gcttctgcca
    13741 cggtatgacc cagcgcggtg acgcacagta cgcgcccgcc gctggttacc acctgctcgt
    13801 catccgccag ttttgtgccc gcgtggaaca ctttgccgtc agccacttct tccagcggca
    13861 ggccgtggat cacatcgccg gtgcggtaat cgcccggata tccacccgca gccatcacca
    13921 cgctgagaga agcacgttca tcccactctg acgttttctc gtccagtttg ccttcacagg
    13981 ccgccaggca gagctcaacc agatcggact tcatgcgcag cataatcggc tgggtttccg
    14041 gatcgccaaa gcggcagtta aattcaataa ccttcggatt gccctgtttg tcgatcatca
    14101 ggcccgcata gagaaaaccg gtgtaggtgt tgccttccgc cgccatgcct ttcacggttg
    14161 gccagatgat acgttccatg gtgcgctgat gaacttcatc ggttactacc ggcgcggggg
    14221 agtaagcgcc catcccgccg gtgtttggtc cgttatcttt atcgcctacg cgtttgtgat
    14281 cctggctggt agccatcggc agcacatgct cgccgtccac catcacgata aagctcgctt
    14341 cttcgccatc gaggaactct tcgataacga tgcgatgacc cgcgtcgcca aaagcgttgc
    14401 ccgccagcat atcgtgaaca gccgcttccg cttcttccag cgtcatcgcc acgataacgc
    14461 ctttcccggc agccagaccg tccgctttaa tgacgattgg cgcgcctttc tcacgcagat
    14521 acaccagcgc aggttctacc tcggtgaagt tctggtattc cgccgtaggg atcttatggc
    14581 gggccaggaa atctttggta aacgcttttg agccttccag ttgggccgca cctgcggttg
    14641 ggccgaagat tttcagcccg gcggcgcgga aggtatcgac cacgcctttc accagcggcg
    14701 cttccgggcc gacgatggtc agatcaatct tttcgttttg tgcgaaatcc aacagcgccg
    14761 ggatatcggt cacgccgata gcaacgtttt gcagcgcggg ttccagcgca gtgcctgcat
    14821 tacccggagc agcaaaaaca gtctcaacca gcggcgactg ggccgctttc caggccagcg
    14881 cgtgctcgcg cccgccgtta ccaatcacta atactttcat ctattgctcc attaatggcg
    14941 gaagtggcgc atgtcggtga agagcatcgc aataccgtgc tcgtcggcgg cggcaatcac
    15001 ttcgtcatca cggatggaac cgccaggctg gattacgcag gtcacgcccg cagcggcggc
    15061 ggcatcaata ccgtcgcgga acgggaagaa cgcgtcagaa gccatcgagg aacctttcac
    15121 ttccaggcct tcatcggcag ctttaatacc ggcgattttc gcggagtaca cgcggctcat
    15181 ctggcccgcg ccaataccga tggtcatatt gtttttggca tagacgatag cattggattt
    15241 cacgaatttc gccactttcc agcagaacag cgcatcacgc agttcctgtt cggtcggctg
    15301 acgttgggtg acgacgcgca gttcttccgc accgaccatc cccaggtcac gatcctgaac
    15361 cagcagaccg ccgttcacgc gtttgaaatc aagacccgga acacgctcgc cccactgacc
    15421 gcaggtcaga acgcgtacgt tctgcttggc ggcggtgatt ttcagggctt cttcgctggc
    15481 ggaaggcgca ataatcactt cgacaaactg acgagaaatg atggcctgtg cggtttccgc
    15541 atccagctcg cggttaaagg caatgatgcc gccgaatgcg gaggttgggt cggttttgta
    15601 cgcgcgatcg taagcatcaa gaatggaatt gccgatagct acaccgcaag ggttggcgtg
    15661 cttcacaatc acacatgccg gctcggcgaa ctctttcacg cactccagcg ccgcatcggt
    15721 atcggcgatg ttgttataag agagggcttt accctgaacc tgggttgcgg tagcaacgga
    15781 ggcttctttc acattctctt ctatatagaa ggcagcctgc tggtggctgt tctcgccgta
    15841 acgcatatcc tgcttcttaa tgaagttcag gttcagcgtg cgtgggaagc gaccggcggc
    15901 ttctttgctt tcaccgtggt acgccggaac catgctgccg aagtagttgg caatcatgct
    15961 gtcgtaggcg gcagtgtgtt cgaaggcttt gatggcgagg tcgaaacggg ttgcaagcgt
    16021 cagcgatcct tcgttgtcat ccatctcttt aataatggcg tcgtagtcgc tgctcttcac
    16081 cacgattgcg acatctttat ggttcttagc ggcggagcgc accatggtcg ggccgccgat
    16141 atcgatgttc tcaaccgcat cttccagcga gcagccttca cgagcaacag tctgggcgaa
    16201 cggatacagg ttaacaacca ccatatcgat aggctggatc tgatgttctt ccataatggc
    16261 atcgtcctgg ccgcgacggc ccagaatgcc accatgtact ttcggatgca gggtcttcac
    16321 gcgtccatcc atcatctccg ggaaaccggt gtaatcggaa acttcggtta ccggcagacc
    16381 tttttctgct aacagacggg cagtgccccc tgtagacagc agctccacac cgcgtgcgga
    16441 aagtgcctgg gcgaattcga cgataccggc tttgtcagaa acactgagca gagcgcggcg
    16501 gactggacga cgttgttgca tggtaaatcc cctgggtttg actattacag agagcgttag
    16561 ctgaattttt cgcgaaaaac tcagctaacg cccctaacgg ggcatcctta tttttcgccc
    16621 gcattgtaac gaaaacgttt gcgcaacgct cgcgaatttt tctctttcaa tggtgatcac
    16681 aattttgaca gtggttaccg tgggaaaaat acagaaatta cattgatgat tgtggataac
    16741 tctgtgcgta aaaaggtata aagcggggtt ttgctgggga atgcagcagt cagtcatttt
    16801 tctgcaattt ttctattgcg gcctgcggag aactccctat aatgcgcctt catcgacacg
    16861 gcggatgtga atcacttcac acaaacagcc ggttcggttg aagagaaaaa tcctgaaatt
    16921 cagggttgac tctgaaagag gaaagcgtaa tatacgccac ctcgcgacag tgcgctaaag
    16981 cgcgtcgcaa ctgctcttta acaatttatc agacaatctg tgtgggcact cgaagatacg
    17041 gattcttaac gtcgcaagac gaaaaatgaa taccaagtct caagagtgaa cacgtaattc
    17101 attacgaagt ttaattcttt gagcatcaaa cttttaaatt gaagagtttg atcatggctc
    17161 agattgaacg ctggcggcag gcctaacaca tgcaagtcga acggtaacag gaagcagctt
    17221 gctg
//
LOCUS       DAZSNM010000079        17221 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZSNM010000079 DAZSNM010000000
VERSION     DAZSNM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17221
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.079"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(41..772)
                     /gene="rlmB"
                     /locus_tag="ACHW42_002138"
     CDS             complement(41..772)
                     /gene="rlmB"
                     /locus_tag="ACHW42_002138"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR8943954.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(952..3393)
                     /gene="rnr"
                     /locus_tag="ACHW42_002139"
     CDS             complement(952..3393)
                     /gene="rnr"
                     /locus_tag="ACHW42_002139"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR8943955.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="ACHW42_002140"
     CDS             complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="ACHW42_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR8943956.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4062..5360)
                     /gene="purA"
                     /locus_tag="ACHW42_002141"
     CDS             complement(4062..5360)
                     /gene="purA"
                     /locus_tag="ACHW42_002141"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR8943957.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5464..5661)
                     /locus_tag="ACHW42_002142"
     CDS             complement(5464..5661)
                     /locus_tag="ACHW42_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR8943958.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="ACHW42_002143"
     CDS             complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="ACHW42_002143"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR8943959.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="ACHW42_002144"
     CDS             complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="ACHW42_002144"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR8943960.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="ACHW42_002145"
     CDS             complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="ACHW42_002145"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR8943961.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="ACHW42_002146"
     CDS             complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="ACHW42_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR8943962.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="ACHW42_002147"
     CDS             complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="ACHW42_002147"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR8943963.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="ACHW42_002148"
     CDS             complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="ACHW42_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR8943964.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="ACHW42_002149"
     CDS             complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="ACHW42_002149"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR8943965.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="ACHW42_002150"
     CDS             complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="ACHW42_002150"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR8943966.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="ACHW42_002151"
     CDS             complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="ACHW42_002151"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR8943967.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15980..17119
                     /gene="queG"
                     /locus_tag="ACHW42_002152"
     CDS             15980..17119
                     /gene="queG"
                     /locus_tag="ACHW42_002152"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR8943968.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17102..17155)
                     /locus_tag="ACHW42_002153"
     CDS             complement(17102..17155)
                     /locus_tag="ACHW42_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8943969.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3884 a         4932 c         4335 g         4070 t
ORIGIN      
        1 aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg
       61 cttcaaataa gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca
      121 ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt
      181 ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg
      241 cctcgcctgc cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg
      301 caaggttagt cacccgaatc agcggaacgc tttctgccgc gccgcaggct acttttttcg
      361 ccgtagcgtt gagctgtgcg gagcgatctt tcggcacaat caccgcatga acgcctgcgg
      421 cgtccgcact acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca
      481 gcaggaacgg ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac
      541 gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga
      601 gatattggcg gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg
      661 gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg
      721 cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac
      781 tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata
      841 tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac
      901 aaagcgcatt ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc
      961 cacttttttc ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc
     1021 cgatggcttt ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc
     1081 accgcggaag gcgctgtctg gctcaaagtt taccttttta ccgacctgac aacgcttgcc
     1141 gccttttttg cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg
     1201 cggtgcgcgt tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc
     1261 ttcgacgcga acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat
     1321 caggcgttgc cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac
     1381 cagaccatca atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga
     1441 aattacgcct ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc
     1501 agcgacatcg cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag
     1561 ttgcaacatc tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg
     1621 cccctgctct ttcgccagca gatatttaat ggcgcggtgc agcgtcaggt caggataacg
     1681 acgaatcggc gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt
     1741 ttctggatcg taaatcgcct gtttcatcga gcgtagcagc atggtttgca gcatttctgc
     1801 atcaggacgg tcggcaaccg actccagcag ttccgcgtag tcacgcggtt ccggcttgtt
     1861 accacccggc agctccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc
     1921 ggtgctcggc ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg
     1981 cgccgccgag atattcgcca gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg
     2041 ctgggtctgt tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc
     2101 gctctcaaat gagatcccac cgcgttcttc acgggcttta tccagcactt tatagaggtt
     2161 atgcaactct tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc
     2221 ctgcagaata tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc
     2281 gtagaatttg tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca
     2341 caggcggtct acctgcgggt tgagcgaaca caggccgtta gagagcactt ccggcagcat
     2401 cgggataacc tgcgaaggga agtacaccga cgtgccacgg ttacgcgctt ctctgtccag
     2461 cggcgttggc ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc
     2521 gccgccgcgt tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc
     2581 aatggtgacc agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac
     2641 ttcttctttc agccccgcaa cctgttgctc aacagcctgc ggccagatat acggaatttc
     2701 atgggtacgc agagcgatat caaccgccat gccggtgccc atattgtcgc ccagcacttc
     2761 gacgatttta cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac
     2821 cacaaagccc atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag
     2881 acggctatcg tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat
     2941 ctggctggtt tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc
     3001 agcacccagc ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct
     3061 ggagagatac aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg
     3121 accaataacg gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg
     3181 agtgaagacc agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag
     3241 ctgctcttcg ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc
     3301 acgtttggtt aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc
     3361 agcttcgcgt tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat
     3421 gaagattttc gtcactccac cagcaataat ttataaagcg gttgattctc ttcaaccaaa
     3481 tcggcaagcg tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc
     3541 tgtttcaacc gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat
     3601 aagggctcca gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg
     3661 cgaatgccgc cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg
     3721 attattttga ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg
     3781 gtcatccgcc cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg
     3841 aaactcgtta actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg
     3901 atgataaacc agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc
     3961 gaggatttaa cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac
     4021 tgttagcccg gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg
     4081 cgcagaatca tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacaccg
     4141 gtcagctctt caatacgctt gatgtagttc agcgccgcct gcggcaggcc gctacgatct
     4201 ttcacgccga aagtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct
     4261 ttccagtcgt cagctgccag cggagtggta gtcacttcgc gaccatccgg catacggtaa
     4321 gccacgcaga gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag
     4381 ccagacaggg agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta
     4441 cgacgacgac gacccgtagt tgcaccgaat tcgttaccct gcttgcagag gaactcgcca
     4501 gtttcatcaa acagttcggc cgggaaagga cctgcaccta cacgagttga gtaagctttg
     4561 aggataccca gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca
     4621 ccagcagtgg tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc
     4681 gtaccctgcg caccttcgaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg
     4741 tcagaaacgt caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga
     4801 actttctggt aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat
     4861 tccatcactt ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc
     4921 agaccgcgac gtgccacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg
     4981 atcgctttcg cgccacgcgc tttctcacgc gcgttatcca gagcaacgtg ataatcaagg
     5041 atcagcggac atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc
     5101 agttctttca tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg
     5161 ctggttacat tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg
     5221 ttgattacga gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc
     5281 cgttcagtca gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt
     5341 acgacgacgt tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat
     5401 cgctttttca gagttacagc actttttgca ctcaaaaatg acctcgatca gtctgttttt
     5461 tgttcagcca atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc
     5521 accgccaaaa cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt
     5581 ccatgccttc gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc
     5641 cagccagatt gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct
     5701 gaggatgcgg tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt
     5761 cggcgtcttc atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt
     5821 gccagagaag ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc
     5881 tttactgaat gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcataat
     5941 gcggccctga cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg
     6001 cagtttttcc gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc
     6061 ggcgcgcata cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat
     6121 acgcacatcg acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac
     6181 tttgcccttc gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc
     6241 cggggtagta acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc
     6301 gagggtcaga cgaccacggg aatcggtgac gatatctttc acgtccaggc gaccaatttc
     6361 agaacgcaga cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc
     6421 accacccgtt gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc
     6481 gacgatcagg tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg
     6541 aatacgtgcg tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagaccagg
     6601 ctcataaacc aaaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat
     6661 accgcgctca ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat
     6721 gataatcgcg ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc
     6781 tgcgcgttgg cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt
     6841 gctccactgg ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta
     6901 tcgctcttcg ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc
     6961 aggttgccac ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc
     7021 gtctcgatat acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt
     7081 ttagcaaagc gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc
     7141 gcctcttcga ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggaatac
     7201 gcttctgctt cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac
     7261 gccgctttta cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata
     7321 cccatgtcat acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg
     7381 gtacgacctt ccgtcagaat gcggtccatg gtgtatttac cgataactcc acgcagggcg
     7441 ctgtcggtag cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc
     7501 ggattggtga cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc
     7561 agcatcacac cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg
     7621 tcgataaacg tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag
     7681 cgtgttacca cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata
     7741 atgacaatcg ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct
     7801 tgcgatgaac tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt
     7861 ttgctcagtt tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg
     7921 tttccatttc cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt
     7981 ccgttattac cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc
     8041 cctgttgtct ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat
     8101 caggtaatcg atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat
     8161 acgaacttgc agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag
     8221 ctgataaaaa cgactcctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc
     8281 cacctcgccg gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt
     8341 ctgtgcggaa agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc
     8401 gaaatcttcc agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc
     8461 gatctcttca agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc
     8521 atcaatgacg tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc
     8581 cgccaccaga tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc
     8641 accgacatct gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc
     8701 tgccgcgtag acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt
     8761 atatcccacc agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc
     8821 acgctgcttt tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa
     8881 acgacggtcg gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg
     8941 tctttcaagg tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag
     9001 ctcaacctgc aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag
     9061 gccggtgcgg tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg
     9121 gctcagggca tggtcaaaaa gaacgaccga agcacccatc gctttgacag cttccgcaat
     9181 ttcaactgct ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt
     9241 aatcacctgc aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc
     9301 ttccatatct ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc
     9361 ataacggtca aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg
     9421 gctccccgtg taaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc
     9481 ggaagtattc tgcgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt
     9541 gttactgtga tgagaaaccg ggcgagatgg gacaacagta gaaatcgcgt gcttgtaaac
     9601 catctggctg accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc
     9661 ttgcagctta ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc
     9721 gttcaggaac ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct
     9781 tatttgtact ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac
     9841 acattcagcc tgcgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt
     9901 tttcactgtc aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg
     9961 ccaactgtct cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt
    10021 caaggtaaga ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca
    10081 aatctcctcg ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat
    10141 gaaaacgctg ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact
    10201 gatgcacctg atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac
    10261 ccgaaatgaa aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc
    10321 ttgctgccgc aaccggatct acctcctgaa gttgacgatg caatgactcc caaccttgct
    10381 ctgccgcctg ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca
    10441 acccttccag caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc
    10501 ccgccgcggt gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt
    10561 aagcctgcga cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag
    10621 cgttcggctt cgccgtcccg atatccatcc ctttgtaaat aaggacagaa tcaacgctta
    10681 tcaactctac tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg
    10741 tcggccccat caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc
    10801 agggctttta tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc
    10861 acaagttgcg gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac
    10921 cactgcgcat gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg
    10981 aatacggact gcttcgccag gtagcctatc agttcaggaa tcaagatttg taaattttgt
    11041 tggcgtaagg gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca
    11101 atacccaatt ccgctagggc agactgcgct ttttctaatg ccgatttttc ttcgccagaa
    11161 acttttagcc gcaacggaat cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc
    11221 aattgtgcct gacgcagcca acgttctgcc actggcaagg ataaaagtga aatgttgcca
    11281 tcgcgctcca gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga
    11341 ctgttcgccg caagtgcagg ttcctgcggt tccggcgctt ttggtttttg catcggcgcg
    11401 ggcgtttgca aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca
    11461 ttcggccagg gggcagccgg acgactgcct gaggcgggcg caggagagta gcgcggagct
    11521 accggctcac gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc
    11581 gggatggcac gcggtgcggg ttgtggttca tcgtccagcg gtagcggcgt ttccagttgc
    11641 tgttgtagca cgctcagcac gccctgatag ataaaatcat gcaccagacg cgactgatgg
    11701 aaacgcactt cgtgtttggc ggggtgtacg ttgacgtcca cctgatgcgg gtcgatctcc
    11761 agatacaaca caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg
    11821 atcgcatgat tgatcaggcg atcgcgcatc atgcgaccat tcacgtagca atactgaatt
    11881 tccgccagtg cgggcgtagt gtgatttgga tcggccaccc agccgcgcag ggtgagatcg
    11941 ccgtgttgcc attcaatcgc cagcgcttgt tcgagaaaag cggtgccgca aatcgcgcct
    12001 aagcgccgtt ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg
    12061 ttatgcgaca ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatttca
    12121 tcaatatggc taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg
    12181 tagaacagat ccagcacctc cagcgtcgtt cccaccggat gcgctgccgg tttaaccgtc
    12241 acgtccatgt cgcgtccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt
    12301 gaagtgagcg tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc
    12361 aggctgataa tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgagcc
    12421 agcgccagcg ccagctcatc ttttttgata ccgcagccgt tatcacgaat gcggataagt
    12481 ttcgccccac cgcgttcgat atcaatatcg atacgcgtcg cacctgcatc gaggctgttt
    12541 tccactagtt ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg
    12601 ttcgccagtt gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg
    12661 cgatctggcg tcgtcaccgt actggcagtt tgtgccgttg caccctgcgg cgcagattgc
    12721 atcggatgcg caaggaagta attgcgcagg cctttgtaaa tggcttctgc cagctgttgt
    12781 tggtaatcgt cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg
    12841 accagtactg atgggatatc cggcgagcgc agaacgccaa ggctggcgtg ttctggtcga
    12901 cgtttatgta tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac
    12961 cctacccgct gggaatgacc gaactgtaaa tccagcaccg cctggcttaa ataggggtca
    13021 gactgactgt tcgccagcac atcacccgcc ccacccagca gctccgacag tttctcgtgc
    13081 tgctccagcc agctggccat ttcactgttg gcgcgacggt tagagagcac ccatacggaa
    13141 gcgccagtcg cactacggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg
    13201 ttttgcttat gtgccacatc actgcgcccc atcaccgaga taaagtaatc cccgtcacgg
    13261 gttaaaacgc ctttaaacat cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg
    13321 gcgatggtga catttttctc ccgcgtacca ccggggccga tagcgccagg gtcctgaccg
    13381 ccgtgtccgg catcaatagc gatgataatt ttatcgccag tgttagccgt cgcgcgcgct
    13441 gccggacgcg ttaccgtatt actgctgata acacccgtag tgcggttact ttccgtttta
    13501 aacggattgc gcgccggttc gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt
    13561 ttcgcaacca cgggcggcgg aggaggcggt ggcggcacat cggcgttaat cgtaaagacg
    13621 acagtgtaat tgctgccatt ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga
    13681 tcgaccacca gccgtagcgt ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc
    13741 agattattgc cgctgaacaa caacggcagt ccctgaatca tgcccgtttg tttgatatcg
    13801 agcgccacgg tgcgtttgct ttgatggcta aacgcataat caggatcgcc aataaaactc
    13861 aacgttatcc gcgcctgttg attaccgtta gaaacctgaa tatcagagag cgtcgcggca
    13921 cccaccggcg tgcacagcag cagcagcgtc gctaccaacc aatttctgat gcgatacatc
    13981 atcccgccac ctttcaaagg ttaaccggct aaacgcgcca gcaacaattc acccgcagag
    14041 gaaaccgcac tcacgcgcgc ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca
    14101 tccgggtcag gaagaacacc tgtaccttgt tgtggccact ccaccaggca gatggcatcg
    14161 ttggcaaaat aatcgcggat ccccataaac tccagctcct cgggatcggc aaggcggtac
    14221 aaatcaaagt gatagaccat taagttgtcg agcgtatagg gttcgaccag cgtataagtg
    14281 gggcttttga cattaccctg atgacccaga gcctgtaaaa agccccggct aaaggtggtt
    14341 ttacctgcgc ctaaatcgcc atacagatag attacggttg cgccatcgca ggctttcgct
    14401 acccgctcgc ccaggtctaa tgttgcctgc tcatcaggga gcggaattac tcgattcatc
    14461 atggttttta tcagtcactt ccgggttaac aatacgctgt agcgtggaaa agagatcggt
    14521 tgccagcatc ccgcgcgttc caaaacgcgc cgccagtacg tcagctgccg caccgtgcgc
    14581 gacacagcct gcacaggctg catcatacgg cgacagtttt tgcccaagca atgcgccaat
    14641 aataccagag agcacatcgc ccatgccgcc gctcgccatg cctgcatttc cggcatcaat
    14701 aatgcctaaa gcgtcaggat gggcggcgac cacggttccg gcacctttca gcaccgctac
    14761 gccgccataa cgttgtacca gacgtttggc gcaatgtaag cggtcacttt caatttcagc
    14821 gacggaacag cctaacaacc gtgcggcctc gctaggatgc ggcgtgatca cgcgattgtg
    14881 acgcttatcg ggattgattg ccagcaggtt caatgcatcg gcatcccaca acatcggttt
    14941 gcgaaaattc tcaacttttt gcagggcttt tttcccccac tcttgctggc ccagaccggg
    15001 accaatcacc accacatcgg cccattccag gctttcggta agagagtcca tagtcagttc
    15061 atgcaccatc aattctggtc gtgcagtcag cagcggcgca atgttctcac tgcgggtcag
    15121 tactcggact aaaccagcac cagcacgtag cgccgcttcc cccgtcatac gaatagcccc
    15181 cgccgtgccg tgatcgccac cgataatcac cagccgcccg tgatcgcctt tatgcgaagt
    15241 cgggcgacgc ggtttcagcc agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt
    15301 ctcctgacct gccagccagc tatccagccc cagtgagtca aaatgcagtt gtcctgtaac
    15361 atcccgcgct tttccagtga gcaagcctag tttcagcgca ataaaagtga tggtgtgatc
    15421 ggcgttgatc actgcgcctg gcgtagcgcc ggtttcagcc agcaggccgg aagggatatc
    15481 aaccgccaca atcggcgcag gatgggaatt agcgtggtcg attaactggc taatggattc
    15541 gcggggcgct tgctgcaagc cggtaccgag cagcgcatca acaatcagat ctaccgattc
    15601 gggccagaca atattcgaag catggatctc gccacccgcg tttaaccatg cttcgcgtgc
    15661 cagcgcggcc tcttccggca acggtttgtc gctctcctgg gccaacaacg tgacctcaat
    15721 gccgaccgct ttggccagtc gcgcgaccac gtagccatcg ccgccgttat taccatgacc
    15781 gcacagcacc agccagtggc gggcgtcagg atacgccgaa cgacacacct ggaatgcggc
    15841 ctcgccagcg cgaagcatca gctcatagag tgtgagcccc agcacatctg ccgcctcgcg
    15901 ttctccgcgg cggatatcgt cggcgtacca gacggtgtgt ggtatactta cggggttttt
    15961 cttcattgta tggtccgtca tgtcagagcc cctcgatctc aatcagttag cgcaaaaaat
    16021 taaacagtgg gggctggaac tgggctttca gcaggtaggt attaccgata ccgatctcag
    16081 cgagtccgag cccaaactgc aagcatggct ggacaaacaa taccacggcg aaatggactg
    16141 gatggcacgt cacggtatgc tgcgcgctcg cccccatgag ttattacccg gtacgctgcg
    16201 tgtaatcagc gtgcggatga attaccttcc tgctaacgcc gcatttgcca gcacgctgaa
    16261 aaaccccaaa ctcggctatg ttagccgtta tgcgctgggc cgtgactatc acaaacttct
    16321 gcgcaaccga ctcaaaaagc tgggcgagat gattcagcaa cattgtgttt cgctgaattt
    16381 tagaccgttt gtcgattctg cgcctattct cgagcgcccg ttagctgaaa aagctgggct
    16441 cggctggaca ggtaagcact cacttatcct caatcgcgag gccggttcgt tcttcttttt
    16501 aggcgaattg ctggtcgata ttccgctgcc cgtggatcaa ccagtcgagg aaggatgcgg
    16561 caaatgcgtg gcctgtatga cgatttgccc gaccggtgcc atcgtcgagc catataccgt
    16621 cgatgctcgc cgctgtatct cttatctcac catcgaactt gaaggggcgc tcccggaaga
    16681 gttgcgaccg ttaatgggaa accgtattta cggttgcgat gactgccagc ttatctgccc
    16741 gtggaatcgc tattcgcaac tcactacaga agacgatttc agcccgcgta agccgctaca
    16801 cgcaccggaa ctcattgagt tatttgcctg gagcgaagag aagtttttaa aagtcacgga
    16861 aggatcggcg attcgccgta taggtcacct gcgctggctg cgtaatatcg ccgtggcatt
    16921 aggcaatgcc ccttgggatg aaacgatttt gacagcgctg gaaagtcgta aaggtgagca
    16981 cccacttctc gatgagcaca tagcgtgggc gattgcgcag caaatagaga gacgaaatgc
    17041 gtgcatagtc gaagtgcaat tgccgaaaaa acagcgtctg gttcgggtga ttgaaaaagg
    17101 gttaccgcgt gacgcctgat tcatccacag cttgtgaata aaaataaaaa cgcattgcaa
    17161 ttcaagacga ataaaatcgt caagtgatcg actcgacaaa acggaatctt gatttttaac
    17221 t
//
LOCUS       DAZSNM010000080        17013 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZSNM010000080 DAZSNM010000000
VERSION     DAZSNM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17013
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.080"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..610
                     /gene="rmuC"
                     /locus_tag="ACHW42_002154"
     CDS             <1..610
                     /gene="rmuC"
                     /locus_tag="ACHW42_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR8943970.1"
                     /translation="YTRESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPV
                     EPAFLLALDRQPELITEALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRA
                     SKLYDKMRLFIDDMSAIGQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKR
                     EINPDLAEQAVSQDEEYRLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            705..1460
                     /gene="ubiE"
                     /locus_tag="ACHW42_002155"
     CDS             705..1460
                     /gene="ubiE"
                     /locus_tag="ACHW42_002155"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR8943971.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            1474..2079
                     /gene="ubiJ"
                     /locus_tag="ACHW42_002156"
     CDS             1474..2079
                     /gene="ubiJ"
                     /locus_tag="ACHW42_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR8943972.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            2076..3716
                     /gene="ubiB"
                     /locus_tag="ACHW42_002157"
     CDS             2076..3716
                     /gene="ubiB"
                     /locus_tag="ACHW42_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR8943973.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            3795..4064
                     /gene="tatA"
                     /locus_tag="ACHW42_002158"
     CDS             3795..4064
                     /gene="tatA"
                     /locus_tag="ACHW42_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR8943974.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            4068..4583
                     /gene="tatB"
                     /locus_tag="ACHW42_002159"
     CDS             4068..4583
                     /gene="tatB"
                     /locus_tag="ACHW42_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR8943975.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            4586..5362
                     /gene="tatC"
                     /locus_tag="ACHW42_002160"
     CDS             4586..5362
                     /gene="tatC"
                     /locus_tag="ACHW42_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR8943976.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            5404..6186
                     /gene="tatD"
                     /locus_tag="ACHW42_002161"
     CDS             5404..6186
                     /gene="tatD"
                     /locus_tag="ACHW42_002161"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR8943977.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(6183..6671)
                     /gene="rfaH"
                     /locus_tag="ACHW42_002162"
     CDS             complement(6183..6671)
                     /gene="rfaH"
                     /locus_tag="ACHW42_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR8943978.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            6838..8331
                     /gene="ubiD"
                     /locus_tag="ACHW42_002163"
     CDS             6838..8331
                     /gene="ubiD"
                     /locus_tag="ACHW42_002163"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR8943979.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            8377..9078
                     /gene="fre"
                     /locus_tag="ACHW42_002164"
     CDS             8377..9078
                     /gene="fre"
                     /locus_tag="ACHW42_002164"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR8943980.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(9270..10433)
                     /gene="fadA"
                     /locus_tag="ACHW42_002165"
     CDS             complement(9270..10433)
                     /gene="fadA"
                     /locus_tag="ACHW42_002165"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR8943981.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(10443..12632)
                     /gene="fadB"
                     /locus_tag="ACHW42_002166"
     CDS             complement(10443..12632)
                     /gene="fadB"
                     /locus_tag="ACHW42_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR8943982.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            12822..14153
                     /gene="pepQ"
                     /locus_tag="ACHW42_002167"
     CDS             12822..14153
                     /gene="pepQ"
                     /locus_tag="ACHW42_002167"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR8943983.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            14153..14767
                     /locus_tag="ACHW42_002168"
     CDS             14153..14767
                     /locus_tag="ACHW42_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR8943984.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            14806..16257
                     /gene="trkH"
                     /locus_tag="ACHW42_002169"
     CDS             14806..16257
                     /gene="trkH"
                     /locus_tag="ACHW42_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR8943985.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            16269..16814
                     /gene="hemG"
                     /locus_tag="ACHW42_002170"
     CDS             16269..16814
                     /gene="hemG"
                     /locus_tag="ACHW42_002170"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR8943986.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         3911 a         4325 c         4681 g         4096 t
ORIGIN      
        1 ctacacccgc gaaagcgcgc tacaggaaca tatcgcgtcg gtgcgtaacc atatccgttt
       61 gctgggacgc aaagattatc aacagctgcc ggggctgcga actctggatt acgtgctgat
      121 gtttattccc gttgaacccg cttttttact ggcgcttgac cgccagccgg agctgatcac
      181 cgaagcgttg aaaaacaata tcatgctggt tagcccgact acgctgctgg tggcgttgcg
      241 tactattgcc aacctgtggc gttatgagca tcaaagccgt aacgcccagc aaatcgccga
      301 tcgtgccagc aagctgtacg acaagatgcg tttgttcatc gatgacatgt ccgcgattgg
      361 tcaaagtctc gacaaagcgc aggataacta ccgccaggca atgaaaaaac tctcttcagg
      421 tcgcggaaat gtgctggcgc aggcagaagc gtttcgcggt ttaggagtag aaattaaacg
      481 cgagattaat ccggatttgg ctgaacaggc ggtgagccag gatgaagagt atcgacttcg
      541 gtcggttccg gagcagccga atgatgaagc ttatcaacgc gatgatgaat ataatcagca
      601 gtcgcgctag cccattaggg gtagttaagc cgggtagaaa tctagggcat cgacgcccaa
      661 tctgttacac ttctggaaca attttttgat gagcaggcat tgagatggtg gataagtcac
      721 aagaaacgac gcactttggt tttcagaccg tcgcgaagga acaaaaagcg gatatggtcg
      781 cccacgtttt ccattccgtg gcatcaaaat acgatgtcat gaatgatttg atgtcatttg
      841 gtattcatcg tttgtggaag cgattcacga ttgattgcag cggcgtacgc cgtgggcaga
      901 ccgtgctgga tctggctggt ggcaccggcg acctgacagc gaaattctcc cgcctggtcg
      961 gagaaactgg caaagtggtc cttgctgata tcaatgaatc catgctcaaa atgggacgcg
     1021 agaagctgcg taatatcggt gtgattggca acgttgagta tgttcaggcg aacgctgagg
     1081 cgctgccgtt cccggataac acctttgatt gcatcaccat ttcgtttggt ctgcgtaacg
     1141 tcaccgacaa agataaagca ctgcgttcaa tgtatcgcgt gctgaaaccc ggcggccgcc
     1201 tgctggtgct tgagttctcg aagccaatta tcgagccgct gagcaaagcc tatgatgcat
     1261 actccttcca tgtgctgccg cgtattggct cactggtcgc gaacgacgcc gacagctacc
     1321 gttatctggc agaatccatc cgtatgcatc ccgatcagga taccctgaaa gccatgatgc
     1381 aggatgccgg attcgaaagt gtcgactact acaatctgac ggcaggggtt gtggcgctgc
     1441 atcgtggtta taagttctga caggagaccg gaaatgcctt ttaaaccttt agtgacggca
     1501 ggaattgaaa gtctgctcaa caccttcctg tatcgctcac ccgcgctgaa aacagcccgc
     1561 tcgcgtctgc tgggtaaagt attgcgcgtg gaggtaaaag gcttttcgac gtcattgatt
     1621 ctggtgttca gcgaacgcca ggttgatgta ctgggcgaat gggcaggcga tgctgactgc
     1681 accgttatcg cctacgccag tgtgttgccg aaacttcgcg atcgccagca gcttaccgca
     1741 ctgattcgca gtggtgagct ggaagtgcag ggcgatattc aggtggtgca aaacttcgtt
     1801 gcgctggcag atctggcaga gttcgaccct gcggaactgc tggcccctta taccggtgat
     1861 atcgccgctg aaggaatcag caaagccatg cgcggaggcg caaagttcct gcatcacggc
     1921 attaagcgcc agcaacgtta tgtggcggaa gccattactg aagagtggcg tatggcaccc
     1981 ggtccgcttg aagtggcctg gtttgcggaa gagacggctg ccgtcgagcg tgctgttgat
     2041 gccctgacca aacggctgga aaaactggag gctaaatgac gccaggtgaa gtacggcgcc
     2101 tatatttcat cattcgcact tttttaagct acggacttga tgaactgatc cccaaaatgc
     2161 gtatcaccct gccgctacgg ctatggcgat actcattatt ctggatgcca aatcggcata
     2221 aagacaaact tttaggtgag cgactacgac tggccctgca agaactgggg ccggtttgga
     2281 tcaagttcgg gcaaatgtta tcaacccgcc gcgatctttt tccaccgcat attgccgatc
     2341 agctggcgtt attgcaggac aaagttgctc cgtttgatgg caagctggcg aagcagcaga
     2401 ttgaagctgc aatgggcggc ttgccggtag aagcgtggtt tgacgatttt gaaatcaagc
     2461 cgctggcttc tgcttctatc gcccaggttc ataccgcgcg attgaaatcg aatggtaaag
     2521 aggtggtgat taaagtcatc cgcccggata ttttgccggt cattaaagcg gatctgaaac
     2581 ttatctaccg tctggctcgc tgggtgccgc gtttgctgcc ggatggtcgc cgtctgcgcc
     2641 caaccgaagt ggtgcgcgag tacgaaaaga ccttgattga tgaactgaat ttgctgcggg
     2701 aatctgccaa cgccattcag cttcggcgca attttgaaga cagcccgatg ctctacatcc
     2761 cggaagttta ccctgactat tgtagtgaag ggatgatggt gatggagcgc atttacggca
     2821 ttccggtgtc tgatgttgcg gcgctggaga aaaacggcac taacatgaaa ttgctggcgg
     2881 aacgcggcgt gcaggtgttc ttcactcagg tctttcgcga cagctttttc catgccgata
     2941 tgcaccctgg caacatcttc gtaagctatg aacacccgga aaacccgaaa tatatcggca
     3001 ttgattgcgg gattgttggc tcgctaaaca aagaagataa acgctatctg gcagaaaact
     3061 ttatcgcctt ctttaatcgc gactatcgca aagtggcaga gctacacgtc gattctggct
     3121 gggtgccacc agataccaac gttgaagagt tcgaatttgc cattcgtacg gtctgtgaac
     3181 ctatctttga gaaaccgctg gccgaaattt cgtttggaca tgtactgtta aatctgttta
     3241 atacggcgcg tcgcttcaat atggaagtgc agccgcaact ggtgttactc cagaaaaccc
     3301 tgctctatgt cgaaggggta ggacgccagc tttatccgca gctcgattta tggaaaacag
     3361 cgaagccttt cctggagtcg tggattaaag atcaggtcgg tattcctgct ctggtgagag
     3421 catttaaaga aaaagcgccg ttctgggtcg aaaaaatgcc agaactgcct gaactggttt
     3481 acgacagttt gcgccagggc aagtatttac agcacagtgt tgataagatt gcccgcgagc
     3541 ttcagtcaaa tcatgtacgt cagggacaat cgcgttattt tctcggaatt ggcgctacgt
     3601 tagtattaag tggcacattc ttgttggtca gccgacctga gtgggggctg atgcccggct
     3661 ggttaatggc aggtggtctg atcgcctggt ttgtcggttg gcgcaaaaca cgctgatttt
     3721 ttcatcgctc aaggcgggcc gtgtaacgta taatgcggct ttgtttaatc atcatctacc
     3781 acagaggaac atgtatgggt ggtatcagta tttggcagtt attgattatt gccgtcatcg
     3841 ttgtactgct ttttggcacc aaaaagctcg gctccatcgg ttccgatctt ggtgcgtcga
     3901 tcaaaggctt taaaaaagca atgagcgatg atgaaccaaa gcaggataaa accagtcagg
     3961 atgctgattt tactgcgaaa actatcgccg ataagcaggc ggatacgaat caggaacagg
     4021 ctaaaacaga agacgcgaag cgccacgata aagagcaggt gtaatccgtg tttgatatcg
     4081 gttttagcga actgctattg gtgttcatca tcggcctcgt cgttctgggg ccgcaacgac
     4141 tgcctgtggc ggtaaaaacg gtagcgggct ggattcgcgc gttgcgttca ctggcgacaa
     4201 cggtgcagaa cgaactgacc caggagttaa aactccagga gtttcaggac agtctgaaaa
     4261 aggttgaaaa ggcgagcctc actaacctga cgcccgaact gaaagcgtcg atggatgaac
     4321 tacgccaggc cgcggagtcg atgaagcgtt cctacgttgc aaacgatcct gaaaaggcga
     4381 gcgatgaagc gcacaccatc cataacccgg tggtgaaaga taatgaagct gcgcatgagg
     4441 gcgtaacgcc tgccgctgca caaacgcagg ccagttcgcc ggaacagaag ccagaaacca
     4501 cgccagagcc ggtggtaaaa cctgctgcgg acgctgaacc gaaaaccgct gcaccttccc
     4561 cttcgtcgag tgataaaccg taaacatgtc tgtagaagat actcaaccgc ttatcacgca
     4621 tctgattgag ctgcgtaagc gtctgctgaa ctgcattatc gcggtgatcg tgatattcct
     4681 gtgtctggtc tatttcgcca atgacatcta tcacttggta tccgcgccat tgatcaagca
     4741 gttgccgcaa ggttcaacga tgatcgccac cgacgtggcc tcgccgttct ttacgccgat
     4801 caagctgacc tttatggtgt cgctgattct gtcagcgccg gtgattctct atcaggtgtg
     4861 ggcatttatc gccccagcgc tgtataagca tgaacgtcgc ctggtggtgc cgctgctggt
     4921 ttccagctct ctgctgtttt atatcggcat ggcattcgcc tactttgtgg tctttccgct
     4981 ggcatttggc ttccttgcca ataccgcgcc ggaaggggtg caggtatcca ccgacatcgc
     5041 cagctattta agcttcgtta tggcgctgtt tatggcgttt ggtgtctcct ttgaagtgcc
     5101 ggtggcaatt gtgctgctgt gctggatggg gattacctcg tcagaagact tacgcaaaaa
     5161 acgcccgtat gtgctggttg gtgcattcgt tgtcgggatg ttgctgacgc cgccggatgt
     5221 cttctcgcaa acgctattgg cgatcccgat gtactgtctg tttgaaatcg gtgtcttctt
     5281 ctcacgcttt tacgttggta aagggcgaaa tcgggaagag gaaaacgacg ctgaagcaga
     5341 aagcgaaaaa actgaagaat aaattcaacc gcccgtcagg gcggttgtca tatggagtac
     5401 aggatgtttg atatcggcgt taatttgacc agttcgcaat ttgcgaaaga ccgtgatgat
     5461 gttgtagcgc gcgcttttga cgcgggagtt aatgggctac tcatcaccgg cactaacctg
     5521 cgtgaaagcc agcaggcgca aaagctggcg cgtcagtatt cgtcctgttg gtcaacggcg
     5581 ggcgtacatc ctcacgacag cagccagtgg caagctgcga ctgaagaagc gattattgag
     5641 ctggccgcgc agccagaagt ggtggcgatt ggtgaatgtg gtctcgactt taaccgtaac
     5701 ttttcgacgc cggaagagca ggaacgcgct tttgttgctc agctacgcat tgccgcagaa
     5761 ttaaacatgc cggtatttat gcactgtcgc gatgcccacg agcggtttat gacattgctg
     5821 gagccgtggc tggataaact gcctggtgcg gttcttcatt gctttaccgg cacacgcgaa
     5881 gagatgcagg cgtgcgtggc gcgtggaatt tatatcggca ttaccggttg ggtttgcgat
     5941 gaacgacgcg ggctggagct gcgggaattg ttgccgttga ttccggcgga gaaattgctg
     6001 atcgaaacag atgcgccgta tctgctcccg cgcgatctca cgccaaagcc atcatcccgg
     6061 cgcaacgagc cagcccatct gccccatatt ttgcaacgta ttgcgcactg gcgtggagaa
     6121 gatgccgcat ggctggctgc caccacggat gccaatgtca aaacactgtt tgggattgcg
     6181 ttttagagtt tgcggaactc ggtgttcttc acactgtgct taatctcttt attaataaga
     6241 ttaagcaata gcatggagcg agcctcacca tcgggttcgg tgaaaatggc ctgaaagcct
     6301 tcgaacgcgc cttcggtaat aatcacctta tcacccggat aagggggggc cggatcgaca
     6361 atgtctttcg gtttatatac cgatagctga tgaattaccg ccgatgggac tatcgctggc
     6421 gacgcgccaa agcgcacaaa gtggctgacg ccacgcgtag cgctgatggt cgtggtatga
     6481 atcacttctg gatcaaattc cacaaacagg tagttaggga ataacggttc actgactgcg
     6541 gtacgtttac cccgtacgat tttttccagg gtgatcatcg gagcgaggca attaaccgcc
     6601 tgtctctcaa ggtgttcctg ggcacgttga agttgcccgc gcttgcagta cagtaaatac
     6661 caggattgca taatgactct tatccgttta atcggggcgc aaggatagca aaagctttac
     6721 gctaagttaa ttatattccc cggtttgcgt tataccgtca gagttcacgc taatttaaca
     6781 aatttacagc attgcaaaga tgaacgccgt ataatgggcg cagattaaga ggctacaatg
     6841 gacgccatga aatataacga tttacgcgac ttcctgacgc tgcttgaaca gcagggtgag
     6901 ctaaaacgta tcacgctccc ggtggatccg catctggaaa tcactgaaat tgctgaccgc
     6961 actctgcgtg ctggtgggcc tgcgctgttg ttcgaaaacc ctaaaggcta ctcaatgccg
     7021 gtgctgtgca acctgttcgg tacgccaaag cgcgtggcga tgggcatggg gcaggaagat
     7081 gtttcggcgc tgcgtgaagt tggtaaatta ttggcgtttc tgaaagagcc ggagccgcca
     7141 aaaggtttcc gcgacctgtt tgataaactg ccgcagttta agcaggtgtt aaacatgccg
     7201 acaaagcgac tgcgtggtgc gccctgccaa caaaaaatcg tctctggcga tgacgtcgat
     7261 ctcaatcgca ttcccattat gacctgctgg ccggaagatg ccgcgccgct gattacctgg
     7321 gggctgaccg taacgcgcgg cccgcataaa gagcggcaga atctgggcat ttatcgccag
     7381 cagctgattg gtaaaaacaa actgattatg cgctggctgt cgcatcgcgg cggcgcgctg
     7441 gattatcagg agtggtgtgc ggcgcatccg ggcgaacgtt tcccggtttc tgtggcgctg
     7501 ggtgccgatc ctgccacgat tctcggtgca gtcacccccg ttccggatac gctttcagag
     7561 tatgcgtttg ccggattgct acgcggcacc aaaaccgaag tagtaaagtg tatttccaat
     7621 gacctcgaag tgccagccag tgccgaaatc gtcctggaag ggtatatcga tcctggtgag
     7681 atggcgccgg aagggccgta tggtgaccac acaggttact ataatgaagt cgataatttc
     7741 ccggtgttta ccgtgacgca tattacccag cgtgaagatg cgatttacca ttccacctat
     7801 accgggcgtc cgccagatga gcccgcggta ctgggcgtgg cgttgaacga agtgtttgta
     7861 ccgattctgc aaaaacagtt cccggaaatt gtcgattttt acctgccgcc ggaaggctgt
     7921 tcttatcgtc tggcggtagt gacgatcaaa aaacagtacg ccggacacgc gaagcgcgtc
     7981 atgatgggcg tctggtcgtt cttacgccag tttatgtaca ctaaatttgt gatcgtttgc
     8041 gatgatgacg tcaacgcacg cgactggaac gatgtgattt gggcgattac cacccgtatg
     8101 gacccggcgc gggatactgt tctggtagaa aatacgccta ttgattatct ggattttgcc
     8161 tcgcctgtct ctgggctggg ttcaaaaatg gggctggatg ccacgaataa atggccgggg
     8221 gaaacccagc gtgaatgggg acgtcccatc aaaaaagatc cagatgttgt cgcgcatatt
     8281 gacgccatct gggatgaact ggctattttt aacaacggta aaagcgcctg atgcgcgttt
     8341 gttttgccct atttatcgat ccgacagaga aagcgcatga caaccttaag ctgtaaagtg
     8401 acctcggtag cagctatcac ggataccgta tatcgtgtcc gcatcgtgcc agacgcggcc
     8461 ttttcttttc gtgctggtca gtatttgatg gtagtgatgg atgagcgcga caaacgtccg
     8521 ttctcaatgg cctcgacgcc ggatgaaaaa gggtttatcg agctgcatat tggcgcttct
     8581 gaaatcaacc tttacgcgaa agcagtcatg gaccgcatcc tcaaagatca tcaaatcgtg
     8641 gtcgaccttc cccacggaga agcgtggctg cgcgatgatg aagagcgtcc gatgattttg
     8701 attgcgggcg gcaccgggtt ctcttatgcc cgctcgattt tgctgacagc gttggcgcgt
     8761 aacccaaacc gtgatatcac catttactgg ggcgggcgtg aagagcagca tctgtatgat
     8821 ctctgcgagc ttgaggcgct ttcgttaaag caccttggtc tgcaagtggt gccggtggtt
     8881 gaacaaccgg aagcaggctg gcgtgggcgt actggtaccg tgttaacggc ggtattgcag
     8941 gatcatggta cgctggcaga gcatgatatc tatattgccg gacgttttga gatggcgaaa
     9001 attgcccgtg acctgttttg cagtgagcgt aatgcgcggg aagatcgcct gtttggcgat
     9061 gcgtttgcat ttatctgaga tataaaaaaa cccgcccctg acaggcggga agaacggcaa
     9121 ctaaaacttt attcagtggc atttagatct atgtcgcatc cggcaattgg tgcacgatgc
     9181 ctgatgcgac gctggcgcgt cttatcaggc ctacattggt gccggatcgg tagaccggat
     9241 aaggcgttca cgccgcatcc ggcaagtggt taaacccgct caaacaccgt cgcaataccc
     9301 tgacccagac cgatacacat cgtcgccaga ccaaactgaa cgtctttgcg ttccatcaga
     9361 ttcagcagcg tggtgctgat acgcgcaccg gaacaaccca gcggatgacc cagcgcgatc
     9421 gcaccaccat tgaggttgat cttctcgtca atctgctcca ttagcccgag atctttaatg
     9481 cacggcagga tctgcgcggc aaaggcttcg ttcatctcaa acacgccgat atcgctggca
     9541 gaaagccccg cttttttcag cgccagcttc gaggccggaa ccgggccgta acccataatc
     9601 gatgggtcac aaccaacgac cgccatcgaa cgcacacgag cgcgcggctt aagacctaat
     9661 tcatgggcgc ggctttcact catcaccagc atggcagctg cgccatcgga aagtgcagaa
     9721 gatgtgcccg ccgttaccgt accgtttact ggatcaaacg ccggacgcag cgtggcgagg
     9781 gcttccacgg tggtttccgg gcgaatcact tcgtcgtaat taaactgctt caggacgccg
     9841 tcggcatcgt gaccaccagt tgggatgatt tcatttttaa atgcggccga ctgcgtggcg
     9901 gcccaggcgc gggcgtgtga ccgcgcggca aaggcatcct gcatttcacg gctgataccg
     9961 tgcatacgcg ccagcatttc tgccgttaag cccatcatgc ccgccgcttt ggcgacattg
    10021 cggctcaggc cggggtgaaa atcgacgccg tgactcatcg gcacatggcc tatatgctcc
    10081 acgccgccaa ccagacatgc ctgcgtatcg ccagtcatga tcattcgtgc tgcgtcatgc
    10141 agtgcctgca tggatgaacc acacaagcga ttgacggtaa ccgccgggac agagtgcggc
    10201 acttctgcca gcagcgccgc gttacgggcg atgttaaaac cctgctccag cgtctgttgc
    10261 acacaacccc agtaaatatc gtcgagggcc gccgcttcca gcgccgggtt acgcgccagc
    10321 aggctacgca ttaaatgagc ggagagatct tccgcacgca cgttacgaaa agcaccgccc
    10381 ttcgaacggc ccatcggggt gcgaattgca tcgacaatga caacctgttc cattgtgact
    10441 ccttaagccg ttttcaggtc gccaaccgga cgggctggct caaccggagg atagtacggt
    10501 tcgttatgac gcgctttatt acgcagacct tccggcactt catacagcgg gccgaggtgc
    10561 tgatattgct gtgccatatc gaggtatttt gcgctaccga gggtgtccag ccagcggaac
    10621 gcgccgccgt ggaacggagg gaagcccagg ccgtagacca gcgccatatc cgcttccgcc
    10681 ggagtggcga taatgccctc ttccagacag cgcaccactt cgttgaccat cgggatcatc
    10741 atgcgggcga taatctcttc ttcgctgaaa tcgcgcttcg gttggctcac ttctgccagc
    10801 aggtcttcaa cggcggcgtc ttcttctttc ttcggcttac ctttgctgtc ttctttataa
    10861 cgccagaaac cgaggccgtt cttctgacca aagcggttgg catcaaacag cgcgtcgatg
    10921 gcatcgcggt aatctttctg catccgctgc gggaagcctg ctgccatgac agcctgagcg
    10981 tgatgcgcgg tatcaatgcc cacaacgtcc agcagatatg ccgggcccat cggccagcca
    11041 aactgttttt ccatcacttt gtcgatcttg cggaaatccg cgccgtcgcg cagcagctgg
    11101 ctgaaaccgg cgaaatacgg gaacagcacg cggttaacaa agaagccggg gcagtcgtta
    11161 accacaatcg gcgtcttgcc catcttgctc gcccaggcga caactttcgc gatggtttcg
    11221 tcggagcttt tctcgccgcg aataatttct accaacggca ttcggtggac cgggttaaag
    11281 aagtgcatcc cgcagaagtt ttccgggcgt tccagcgcgt tggccagttc gctgatagga
    11341 atggttgaag tgttagacgc cagcacggta tcctggcgta ctttttgttc ggtttctgcc
    11401 agtacggctt ttttcacttt cgggttttca acaaccgctt ctaccacaat atccacgcgg
    11461 tcaaatccgg cgtagtcgag cgttgggtgg atcgtggaga tcacgccagc cagtttcaga
    11521 ccatcgattt tgccgcgctc aagctgcttg ttcagcagtt tcgcggcttc ggtcatgccg
    11581 agggttaacg acttgtcgtt gatatctttc atgacaaccg gcacgccttt ccacgcggac
    11641 tggtaagcga taccgccacc catgatgcct gcgcccagca cagcagcctg tttcggggtt
    11701 tcaacgtctt tggtgagttt cttcgctttg ccttttacat attgatcgtt aaggaaaatg
    11761 ccgaccagtg cgcgagcttc gttggtatgc gccagcggga caaaactttt gttttccaga
    11821 tttaaggctt cttcacgacc aaaacgggcc gcagcttcaa tggtttttac tgcggtgatg
    11881 ggggccggat aatgtttccc cgccgtttgc gcgaccattc ctttagcgat ggtgaagctc
    11941 atggtggctt caatcttgct cagttttagc ggttcctgct tcggctgacg tttcgctttc
    12001 cagtcgaggt cgccgttaat ggcctggcgt aaaatcgcca tcgccccttc aatcagtttt
    12061 tctgctttga ctacgccatc caccagaccg attttcagcg cctgatccgc gccgacatct
    12121 ttaccggcgg caatgatttc cagtgcactg tcagcgccca gcatacgtgg catacgtaca
    12181 gaaccgccaa agcccggcat gatgcccagt ttggtttccg gcagaccgat gcgcagatcc
    12241 ggcgtcgcca gacgataatc ggtcgccagc acgcattcgc agccaccacc cagcgcatag
    12301 ccgttgacgg cagcaatggt cggcaccggc agatcttcca ggcgattaaa cacgctattg
    12361 gcaaagtgca gccactgact taactgttct tcaggaacga ggaacaggga caaaaattcg
    12421 gtgatatcag caccgacgat aaaggctgct ttgttcgaac gcagcagcag cccttttaga
    12481 tctgattgct gttccagcac gccgatggcc tcgccgaggc tggcgacggt cgcagtgtcg
    12541 agtttattaa ctgaacctgg ggcatcaaat accagttcgg caatgccatc ttccagccag
    12601 tcaaggtaca gggtgtcgcc tttgtaaagc atgtcagtct cctgaatccg caaggtaatc
    12661 tggtcgtacc agatgcatcg gagtgtgtgt tttgtgttaa aaatatgcaa ataaaagatt
    12721 aaagaaatgc cgatctgatc acgctcggca gaaatcacgc tctggatgaa cgatgtgcta
    12781 agatgcggag acttaaggtc aaaaaaacag aagggtaaaa aatggaatca ctggcctcgc
    12841 tctataaaaa tcatatagct accttacaag aacggactcg cgatgcgctg gcgcgcttca
    12901 agctggatgc attacttatt cactccggcg agctgttcaa cgtttttctc gacgatcatc
    12961 cctatccgtt taaagtgaac ccgcaattca aagcgtgggt gccggtaact caggtgccaa
    13021 actgctggtt gctggtggat ggcgtgaaca agccgaaact gtggttctat ctgccggttg
    13081 attactggca caacgtcgaa ccgctgccga cctccttctg gactgaagat gtggaagtga
    13141 tcgcgctgcc gaaagccgat ggcattggta gcctgctgcc tgctgcgcgc ggcaatatcg
    13201 gttatatcgg tccggtgccg gaacgtgcgc tgcaactggg tattgaggcc agcaacatca
    13261 acccgaaagg ggtgatcgac tacctgcatt attaccgctc cttcaaaacc gagtacgaac
    13321 tggcctgtat gcgtgaagcg cagaaaatgg cggtcaatgg tcatcgtgcg gcagaagaag
    13381 cgttccgttc tggcatgagc gagttcgata tcaatattgc ctatctgacc gcgaccggtc
    13441 atcgtgatac cgacgtacct tacagcaaca ttgtggcgct taacgaacac gctgcggtgt
    13501 tgcattacac caaactggac caccaggcac cggaagagat gcgcagcttc ctgctggatg
    13561 ccggggccga atataacggc tatgcggctg acctgacccg tacctggtcg gcaaaaagcg
    13621 acaacgacta cgcacagctg gtgaaagacg taaatgatga acaactggcg ctgatcgcca
    13681 ccatgaaagc aggcgtcagc tatgtggatt accacatcca gttccatcag cgcatcgcca
    13741 aactgctgcg taaacatcaa atcatcaccg atatgagtga agaagcgatg gtcgaaaacg
    13801 atctcaccgg accgtttatg ccgcacggta ttggccatcc gctgggcctg caggtgcatg
    13861 acgtcgccgg ttttatgcag gatgatagcg gtacacacct cgcggcaccg gcaaaatatc
    13921 cgtacctgcg ctacacccgt attctccagc cgggcatggt gttaaccatc gaaccgggta
    13981 tctacttcat tgaatcgctt ctggcaccgt ggcgtgaagg gcagttcagc aagcacttca
    14041 actggcagaa aattgaagca ctgaaaccgt tcggcggcat tcgtatcgaa gacaacgtgg
    14101 tgatccacga aaacaacgtg gaaaacatga cccgggatct gaaactggcg tgatggaaag
    14161 ctggttaatt cctgcggcac cggtcacggt cgttgaagag atcaaaaaga gccgcttcat
    14221 aacgatgttg gcgcataccg atggcgttga ggcggcgaaa gcgtttgttg aatcggtgcg
    14281 ggcagaacac cccgatgccc gccaccattg cgtggcgtgg gtggcgggtg cgccggatga
    14341 ttctcaacag ctgggtttct ctgacgacgg ggagccggcg ggaacggcag gtaaaccgat
    14401 gctcgcccag ctaatgggca gcggcgtcgg ggaaattacc gctgtggtag tgcgctacta
    14461 cggcggcata ttgcttggca ccggtgggtt agtgaaagcg tatggcggcg gcgtgaatca
    14521 ggcgctgcgc cagctaacga cccaacgcaa gacgccatta accgaatata ctttgcaatg
    14581 tgaatatagt cagttaaccg gcattgaagc gttgctgggg cagtgtgacg gcaaaattat
    14641 caacagtgat tatcaggcat tcgttctgct gcgggtggcg cttccggcgg cgaaagtggc
    14701 tgaattttcc gcaaagctgg cggattttag ccgtggttca ttgcaattgt tagcgattga
    14761 agaataatcc ccacttcgtt ttgcagaact aaggaagcgg cagagatgca ttttcgcgcc
    14821 attacccgaa tcgttggact actggtcatc ttattttcag ggaccatgat tatccctggg
    14881 ctggtagcac tcatctaccg ggatggagcg ggccgcgctt ttacccagac cttttttgtc
    14941 gccctcgcca ttggctctat gctgtggtgg ccgaaccgca aagagaaggg cgaactgaaa
    15001 tcccgtgagg ggtttctgat agtggtgctg ttttggaccg tgctgggtag tgtcggtgcg
    15061 ctccctttta tcttctcgga aagcccgaac ctcacgatta ccgatgcgtt ttttgaatct
    15121 ttctctggcc tgaccaccac cggggccact acgctggtgg ggctggattc gctccctcat
    15181 gccatcctct tttatcgcca gatgctgcaa tggtttggcg ggatggggat catcgtgtta
    15241 gcggttgcga tactgcctat cctcggcgtg ggtgggatgc agctctatcg cgcagaaatg
    15301 cccggcccgc tgaaagataa caaaatgcgc ccgcgaattg cggaaacggc gaaaactctg
    15361 tggttgattt atgtcttgct gaccgtcgcc tgtgcgctgg cgttgtggtt tgccggaatg
    15421 gatgcctttg atgccatcgg ccatagcttt gcgactatcg ctattggcgg cttctcgaca
    15481 catgatgcca gtatcggtta tttcgacagt ccgactatta acactatcat tgctatcttc
    15541 ctgctgatct ccggctgtaa ctacggtctg cacttttcac tgttaagtgg gcgtagtctg
    15601 aaggtttatt ggcgcgatcc ggaatttcgc atgtttatcg gcgtacagtt tacgctggtg
    15661 gttatttgta cactcgtact gtggtttcat aatgtctaca gttcggcgct gatgacaatt
    15721 aaccaggcgt ttttccaggt ggtatcgatg gcgacaaccg ccgggtttac gactgacagc
    15781 attgcccgct ggccgctctt tttgccggta ctgcttttat gttcagcttt tatcggcggt
    15841 tgcgccgggt caacgggcgg tggcctgaaa gtgatccgca tcctgctgct gtttaagcag
    15901 gggaaccgtg agctgaaacg actggtgcat ccgaacgccg tctatagcat taagctgggg
    15961 aatcgcgcac tgccggaacg tatcctcgaa gccgtttggg gatttttctc cgcctatgca
    16021 ttggtgttta ttgtcagtat gctggcgatt atcgccacgg gcgtggatga tttttctgcc
    16081 tttgcctcag ttgttgcgac attgaataac ctggggccag ggcttggcgt ggttgctgat
    16141 aactttacca gtatgaaccc ggtggctaaa tggatcctga ttgccaacat gctgtttggt
    16201 cgtctcgagg tctttacatt actggtgctc tttaccccga ctttctggcg tgaatgatgg
    16261 agtaatacgt gaaaacatta attcttttct caacaaggga cggacaaacg cgcgagattg
    16321 cctcctacct ggcttcggaa ctgaaagaac tggggatcca gacggatgtc gccaatgtgc
    16381 accgcattga agaaccacaa tgggaaaact atgaccgtgt ggtcattggt gcttctattc
    16441 gctatggtca ctaccactct gcgttccagg aatttgtcaa aaaacatgcg acgcgactga
    16501 attcgatgcc gagcgccttt tattccgtaa acctggtggc gcgcaaaccg gagaagcgta
    16561 ctccacagac caacagctac gcgcggaagt ttctgatgaa ctcgcaatgg cgtcccgatc
    16621 gctgcgcggt cattgccggg gcgctgcgtt acccacgtta tcgctggtac gaccgtttta
    16681 tgctcaagct gattatgaag atgtcaggcg gtgaaacgga tacgcgcaaa gaagttgtct
    16741 ataccgattg ggagcaggtg gcgaatttcg cccgagaaat cgcccattta accgacaaac
    16801 cgacgctgaa ataagcataa agaataaaaa atgcgcggtc agaaaattat tttaaatttc
    16861 ctcttgtcag gccggaataa ctccctataa tgcgccacca ctgacacgga acaacggcaa
    16921 acacgcagcc gggtcagcgg ggttctcctg agaattccga cagagaaagc aaaaataaat
    16981 gcttgacttt gtagcgggaa agcgtattat gaa
//
LOCUS       DAZSNM010000081        16358 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZSNM010000081 DAZSNM010000000
VERSION     DAZSNM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16358
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.081"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1492)
                     /locus_tag="ACHW42_002171"
     CDS             complement(<1..1492)
                     /locus_tag="ACHW42_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR8943987.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLM"
     gene            complement(1579..2229)
                     /gene="hxpA"
                     /locus_tag="ACHW42_002172"
     CDS             complement(1579..2229)
                     /gene="hxpA"
                     /locus_tag="ACHW42_002172"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFR8943988.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(2240..2734)
                     /locus_tag="ACHW42_002173"
     CDS             complement(2240..2734)
                     /locus_tag="ACHW42_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR8943989.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(2817..3272)
                     /gene="yfbV"
                     /locus_tag="ACHW42_002174"
     CDS             complement(2817..3272)
                     /gene="yfbV"
                     /locus_tag="ACHW42_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR8943990.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            3610..4812
                     /gene="ackA"
                     /locus_tag="ACHW42_002175"
     CDS             3610..4812
                     /gene="ackA"
                     /locus_tag="ACHW42_002175"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR8943991.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            4887..7031
                     /gene="pta"
                     /locus_tag="ACHW42_002176"
     CDS             4887..7031
                     /gene="pta"
                     /locus_tag="ACHW42_002176"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR8943992.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            7221..8741
                     /gene="yfcC"
                     /locus_tag="ACHW42_002177"
     CDS             7221..8741
                     /gene="yfcC"
                     /locus_tag="ACHW42_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR8943993.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(8774..9316)
                     /gene="yfcD"
                     /locus_tag="ACHW42_002178"
     CDS             complement(8774..9316)
                     /gene="yfcD"
                     /locus_tag="ACHW42_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR8943994.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(9374..9925)
                     /gene="yfcE"
                     /locus_tag="ACHW42_002179"
     CDS             complement(9374..9925)
                     /gene="yfcE"
                     /locus_tag="ACHW42_002179"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR8943995.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(9981..10625)
                     /gene="yfcF"
                     /locus_tag="ACHW42_002180"
     CDS             complement(9981..10625)
                     /gene="yfcF"
                     /locus_tag="ACHW42_002180"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR8943996.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            10761..11408
                     /gene="yfcG"
                     /locus_tag="ACHW42_002181"
     CDS             10761..11408
                     /gene="yfcG"
                     /locus_tag="ACHW42_002181"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR8943997.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            11465..11827
                     /gene="folX"
                     /locus_tag="ACHW42_002182"
     CDS             11465..11827
                     /gene="folX"
                     /locus_tag="ACHW42_002182"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR8943998.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            11848..12741
                     /locus_tag="ACHW42_002183"
     CDS             11848..12741
                     /locus_tag="ACHW42_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR8943999.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(12789..13679)
                     /gene="rpnB"
                     /locus_tag="ACHW42_002184"
     CDS             complement(12789..13679)
                     /gene="rpnB"
                     /locus_tag="ACHW42_002184"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR8944000.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(13876..14649)
                     /gene="hisP"
                     /locus_tag="ACHW42_002185"
     CDS             complement(13876..14649)
                     /gene="hisP"
                     /locus_tag="ACHW42_002185"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR8944001.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(14657..15373)
                     /locus_tag="ACHW42_002186"
     CDS             complement(14657..15373)
                     /locus_tag="ACHW42_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8944002.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(15370..16056)
                     /gene="hisQ"
                     /locus_tag="ACHW42_002187"
     CDS             complement(15370..16056)
                     /gene="hisQ"
                     /locus_tag="ACHW42_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR8944003.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(16146..>16358)
                     /locus_tag="ACHW42_002188"
     CDS             complement(16146..>16358)
                     /locus_tag="ACHW42_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFR8944004.1"
                     /translation="FLKQPVGKDYKFGGPSVKDEKLFGVGTGMGLRKEDNELREALNK
                     AFAEMRADGTYEKLAKKYFDFDVYGG"
BASE COUNT         4059 a         4390 c         3994 g         3915 t
ORIGIN      
        1 ccatcagccc tttcaccgcc agcgcgacac ctcccgtttt ctgtaatgcc acagcaaacg
       61 gcatcatccc aacgatcaaa ataatgctcg gccagtgaat ggatttatag gcgctttcag
      121 catctataca gcggaatttc cccatcagca ggcaggcgat gatagcggca acaggattag
      181 gaatttcatt tgtcagcatt aacgccacca ttagcaccag acagaaaatg gcatggggtg
      241 cctggctgtg cgcgggcgat gcttcactca cctcttccgg taagttcagc gctacgaagt
      301 cgcggccctg tttggccagc ataccgatca gtttccagtt acccacaacc aggatgatat
      361 cgcccagcag cagaggctca tccgccagcg aaccttccag cgccacgcca ttgcgcttga
      421 gccccaccac attcagtccg tagcgggtac gaaaaccaat ttcgcgcacc gatttaccaa
      481 tcagttctga ctcaggaatt aatgaaatct ctgccatgcc cacatcaagg gcctggtcag
      541 aaaaatactc gccgcgcagt accatcggct ccagcaattg ctcactacaa aattgccgga
      601 gatcgacatc agccgcagac atatcaataa gcaaaacgtc acgcgcgcga aattcagaaa
      661 ccccattaac gttcacgata acgcgacgaa aacgccgcca gcgttcaaca ccgatgacgt
      721 tagcgccata acgctcacgt aatttgagat catccagccg ttgacctatc attggcgatc
      781 cggggcgaat agccagacgt cgcgcacgcc cggtcagtcg atattcacgg ataagatcgc
      841 gaaaggttcg acgcgtccag ccttcgcgct gcggggtctg ggtatcccct ttcagcatga
      901 aacgcatcac taacatatac aaaataccca gcaccagcac gaccaggcca ataggtgtta
      961 cgctaaagaa actgaagccg tgatagcctt cacgcagcaa ttcactgttg actaccaggt
     1021 tcggcggcgt cgccaccagc gtcatcatgc cgctaatcag cccggcaaaa cttaacggca
     1081 tcatcagacg tgacggcgaa gtttgcatac gcatggcaac gcttaacacc acgggaataa
     1141 agatagcgac aacgctggtt gaactcataa acgcgccaag ccccgcgacg gtcagcatca
     1201 acaaaaccaa cattttgatt tcactgttgc ccgccacttt gaccagccat gttcccatta
     1261 cggtggcaac accggtacgg accaaaccat cgccaataat aaacaaggcg gcaatcagga
     1321 caacgttagg atcagaaaag ccggaaaata cttcggggac cgtcagcgtt ccgcttaatg
     1381 caaacgcgac aataacaaac aaagcgaccg catccatacg cactctgccc gtcgcaaaca
     1441 agacgatggc aacggccagt aatgaaagaa cccaaatcaa ttcaccgttc acaacttatc
     1501 cttgttaatt gagggggatg acttgattct gccataaaaa agccccgacg agacggggct
     1561 aaatcatgat caggtgtttc actgaataat aacatcgcca tttggctgtt tggtcacagt
     1621 aatttgctcc agactgtgga ggaccaaatc gacctcattc aggcgcgggg tatctgccgg
     1681 agcgttaacc gcaatgacat ggcaacccgc cgccaggcca gaaagcacgc cagcgggagc
     1741 atcttccacc accacacact cctgcggcgc aagccccagc agctgcgcac ctaacagata
     1801 cgcatcaggt tctggttttc cgcgcttcac tcgctcagcg gttacaaaca cctctggtgc
     1861 gggaagcccc gctattttat ggcgcgctcg cgctaccggc atggagccag aagtcacaat
     1921 ggcccacgga atacctgctt tattcaaatg attgagtaag gcgattgccc caggaagcgc
     1981 ggtaatacct tcggtttccg tggcctcgat ttgctccaga cgcgtaaact cggcggcaat
     2041 atcagcctcg gatttgcccg ccataaaatg gcgcaaagag gtgatcgcct gtttaccgtg
     2101 aatgaaagcc agcacctctt ccggcgctaa cccatgacgt ctggcccagt tgctccacgc
     2161 ccgttctacc gcaggcaggg aatccaccag cgttccatca agatcaaaca gaaaaccttt
     2221 gcaccgcacg cgggcctcct caggcgttga taatttgatt aatttcgttg gcgctcaaat
     2281 ggtactgacg cgggcaggca tgccacacat taagcatgcg ctgatatttt tcccacattg
     2341 gcgtctgggc gttaaaacca tgagtcccag cgtcaaaatg ggtatagcgc ccttccacat
     2401 taaccataaa gcggacataa ccgaggtaac gtgcttcagt ggcggcgtca aagccgagga
     2461 aggtgacacg acgttcatcg atggattgct ggtcctgcaa attagaccaa gaaacataca
     2521 acgcatgata catatccata atgtcgatga tagtgcggca ggtttcttct ttcagctcgc
     2581 caaactcgcg atccaattca cgcatctgta atccgtaacc acgctcaata attgtttgca
     2641 ggcgacggta acgttcagca tttgccggat cgagcatagt catcatcttg tactgattag
     2701 acaaaataag acgttgcgcg ttggtcattt ccattgttga ctcctgtatc actctactac
     2761 ggtgaaaaaa aagaaggctg agtatgcctt cttttatatg cgtaatcagg ggtcaattac
     2821 aaatcatcaa ggaaagtttt atccagttgt ttgaaggcgc gcttaagcgt gtcagctaat
     2881 gcctggtaat caggcttgcc ttcaacgggt gccaacacct gtccagactc ctgcaattta
     2941 ccgcgaactt cataaaacca gttaaggatt gcagggggta atggcgttac agaacgcttg
     3001 cccagccacc acaatccctg catgggtaaa cttaaggcga acagtgcagt ggcaactgcc
     3061 ggcccaagca gaccgcccag ggcaatctgc cagcagagag taaatacggc gatcggcggc
     3121 ataaaacgga tcgcataacg cgtcatcttg ataacgcgat tttcgacaaa gaccggagca
     3181 aggcgttttt ccagtggcca cgtctttgag taatgctgtc cgcggcgaaa caagctaaaa
     3241 aaattaacag aacgattatc cggcgttgac atgcttcacc tcaacttcac atataaagat
     3301 tcaaaaattt gtgcaaattc acaactcagc gggacaacgt tcaaaacatt ttgtcttcca
     3361 tacccactat caggtatcct ttagcagcct gaaggcctaa gtagtacata ttcattgagt
     3421 cgtcaaattc atatacatta tgccattggc tgaaaattac gcaaaatggc atagactcaa
     3481 gatatttctt ccatcatgca aaaaaaattt gcagtgcatg atgttaatca taaatgtcgg
     3541 tgtcatcatg cgctacgctc tatggctccc tgacgttttt ttagccacgt atcaattata
     3601 ggtacttcca tgtcgagtaa gttagtactg gttctgaact gcggtagttc ttcactgaaa
     3661 tttgccatca tcgatgcagt aaatggtgaa gagtaccttt ctggtttagc cgaatgtttc
     3721 cacctgcccg aagcacgtat caaatggaaa atggacggca ataaacagga agcggcttta
     3781 ggtgcaggcg ccgctcacag cgaagcgctc aactttatcg ttaatactat tctggcacaa
     3841 aaaccagaac tgtctgcgca gctgactgct atcggtcacc gtatcgtaca cggcggcgaa
     3901 aagtatacca gctccgtagt gatcgatgag tctgttattc agggtatcaa agatgcagct
     3961 tcttttgcac cgctgcacaa cccggctcac ctgatcggta tcgaagaagc tctgaaatct
     4021 ttcccacagc tgaaagacaa aaacgttgct gtatttgaca ccgcgttcca ccagactatg
     4081 ccggaagagt cttacctcta cgccctgccg tacaacctgt acaaagagca cggcatccgt
     4141 cgttacggcg cgcacggcac cagccacttc tatgtaaccc aggaagcggc aaaaatgctg
     4201 aacaaaccgg tagaagaact gaacatcatc acctgccacc tgggcaacgg tggttccgtt
     4261 tctgctatcc gcaacggtaa atgcgttgac acctctatgg gtctgacccc gctggaaggt
     4321 ctggtcatgg gtacccgttc tggtgatatc gatccggcga tcatcttcca cctgcacgac
     4381 accctgggca tgagcgttga cgcaatcaac aaactgctga ccaaagagtc tggcctgctg
     4441 ggtctgaccg aagtgaccag cgactgccgc tatgttgaag acaactacgc gacgaaagaa
     4501 gacgcgaagc gcgcaatgga cgtttactgc caccgcctgg cgaaatacat cggtgcctac
     4561 actgcgctga tggatggtcg tctggacgct gttgcattca ctggtggtat cggtgaaaat
     4621 gccgcgatgg ttcgtgaact gtctctgggc aaactgggcg tgctgggctt tgaagttgat
     4681 catgaacgca acctggctgc acgtttcggc aaatctggtt tcatcaacaa agaaggtacc
     4741 cgtcctgcgg tggttatccc aaccaacgaa gaactggtta tcgcgcaaga cgcgagccgc
     4801 ctgactgcct gatttcacac cgccagctca gctggcggtg ctgttttgta acccgccaaa
     4861 tcggcggtaa cgaaagagga taaaccgtgt cccgtattat tatgctgatc cctaccggaa
     4921 ccagcgtcgg tctgaccagc gtcagccttg gcgtgatccg tgcaatggaa cgcaaaggcg
     4981 ttcgtctgag cgttttcaaa cctatcgctc agccgcgtac cggtggcgat gcgcccgatc
     5041 agactacgac tatcgtgcgt gcgaactctt ccaccacgac ggccgctgaa ccgctgaaaa
     5101 tgagctacgt tgaaggtctg ctttccagca atcagaaaga tgtgctgatg gaagagatcg
     5161 tcgcaaacta ccacgctaac accaaagacg ctgaagtcgt tctggttgaa ggtctggtcc
     5221 cgacacgtaa gcaccagttt gcccagtctc tgaactacga aatcgctaaa acgctgaatg
     5281 cggaaatcgt cttcgttatg tctcagggca ctgacacccc ggaacagctg aaagagcgta
     5341 tcgaactgac ccgcaacagc ttcggtggtg ccaaaaacac caacatcacc ggcgttatcg
     5401 ttaacaaact gaacgcaccg gttgatgaac agggtcgtac tcgcccggat ctgtccgaga
     5461 ttttcgacga ctcttccaaa gctaaagtaa acaatgttga tccggcgaag ctgcaagaat
     5521 ctagcccgct gccggttctc ggcgctgtgc cgtggagctt tgacctgatc gcgactcgtg
     5581 cgatcgatat ggctcgccac ctgaatgcga ccatcatcaa cgaaggcgac atcaatactc
     5641 gccgcgttaa atccgtcact ttctgcgcac gcagcattcc gcacatgctg gagcacttcc
     5701 gtgccggttc tctgctggtg acttccgcag accgtcctga cgtgctggtg gccgcttgcc
     5761 tggcagccat gaacggcgta gaaatcggtg ccctgctgct gactggcggc tacgaaatgg
     5821 acgcgcgcat ttctaaactg tgcgaacgtg ctttcgctac cggcctgccg gtatttatgg
     5881 tgaacaccaa cacctggcag acctctctga gcctgcagag cttcaacctg gaagttccgg
     5941 ttgacgatca cgaacgtatc gagaaagttc aggaatacgt tgctaactac atcaacgctg
     6001 actggatcga atctctgact gccacttctg agcgcagccg tcgtctgtct ccgccagcgt
     6061 tccgttatca gctgactgaa ctggcgcgca aagcgggcaa acgtatcgtt ctgccggaag
     6121 gtgacgaacc gcgtaccgtt aaagcagccg ctatctgtgc tgaacgtggt atcgcaactt
     6181 gcgtactgct gggtaatccg gcagagatca accgtgttgc agcgtctcag ggtgtagaac
     6241 taggtgcagg cattgaaatc gttgatccag aagtggttcg cgaaagctat gttggtcgtc
     6301 tggtcgagct gcgtaagaac aaaggcatga ccgaaaccgt tgcccgcgaa cagctggaag
     6361 acaacgtggt gctcggtacg ctgatgctgg aacaggatga agttgatggt ctggtttccg
     6421 gtgctgttca cactaccgca aacaccatcc gtccgccgct gcagctgatc aaaactgcac
     6481 cgggcagctc cctggtatct tccgtgttct tcatgctgct gccggaacag gtttacgttt
     6541 acggtgactg tgcgatcaac ccggatccga ccgctgaaca gctggcagaa atcgcgattc
     6601 agtccgctga ttccgctgcg gccttcggta tcgaaccgcg cgttgctatg ctctcctact
     6661 ccaccggtac ttctggtgca ggtagcgacg tagaaaaagt tcgcgaagca actcgtctgg
     6721 cgcaggaaaa acgtcctgac ctgatgatcg acggtccgct gcagtacgac gctgcggtaa
     6781 tggctgacgt tgcgaaatcc aaagcgccga actctccggt tgcaggtcgc gctaccgtgt
     6841 tcatcttccc ggatctgaac accggtaaca ccacctacaa agcggtacag cgttctgccg
     6901 acctgatctc catcgggccg atgctgcagg gtatgcgcaa accggttaac gacctgtccc
     6961 gtggcgcact ggttgacgat atcgtctaca ccatcgcgct gactgcgatt cagtctgcac
     7021 agcagcagta atctcgtcat catccacagc tttgcgctgc ggatatctga accggaaata
     7081 atcactattt ccggtttttt attctcttaa tttgcattaa tcctttctga ttatcttgct
     7141 taactgcgct gcatcaatga attgcgccat ctcactttgc atacttacca ctttgttttg
     7201 tgcaagggaa tatttgcgct atgtccgcaa tcactgaatc caaaccaaca agaagatggg
     7261 caatgcccga tacgttggtg attatctttt ttgttgctat tttaaccagt cttgccacct
     7321 gggtagttcc ggtggggatg tttgacagtc aggaagtgca gtatcaggtt gatggtcaaa
     7381 caaaaacacg caaagtcgta gatccacact catttcgcat tctgactaac gaagcaggcg
     7441 aacctgagta tcaccgcgta cagctgttca cgacgggcga tgaacgcccg ggcctgatga
     7501 acttcccgtt tgaagggtta acctcaggat cgaaatacgg gacagccgtt ggcatcatca
     7561 tgtttatgct ggtgattggc ggcgcgtttg gcattgtgat gcgtacagga accattgata
     7621 acggtatcct ggcgcttatt cgccataccc gcgggaatga aattctcttt attcctgcgc
     7681 tgtttattct gttttcactt ggcggcgcgg tatttggtat gggagaagag gccgtcgcct
     7741 ttgccattat catcgcaccg ctaatggtcc ggctgggcta tgacagtatt accaccgtcc
     7801 tggtgactta tattgccacg caaatcggtt ttgccagttc gtggatgaac ccgttttgtg
     7861 tggttgttgc tcaggggatt gccggcgttc cggtgctttc tggctccggg ttgcgcatcg
     7921 tggtgtgggt tatcgccact ctgattggcc tgatctttac catggtgtac gcctcacgag
     7981 tgaaaaagaa tcctcttctg tcacgcgtgc atgagtccga ccgcttcttt cgtgaaaagc
     8041 aggcggatgt tgaacaacgt ccgtttacct ttggtgactg gctggtattg attgtcctga
     8101 cagccgtaat ggtctgggtg atttggggcg tgatcgttaa tgcctggttt attccagaaa
     8161 ttgccagcca gttcttcacc atgggtctgg tgattggcat catcggcgtt gttttccgcc
     8221 ttaacggcat gacggttaat accatggctt catcctttac cgaaggggcg cgaatgatga
     8281 tcgcccctgc cctgctggtg ggtttcgcca aagggatttt gctgctggtt ggtaatggtg
     8341 aagcaggtaa tgccagcgtg ttaaatacta tcctcaacag cattgccaat gccattagcg
     8401 gtctggacaa cgcggtcgcg gcctggttta tgttgctctt ccaggctgta tttaatttct
     8461 tcgtgacgtc cggttctggt caggcggcat taactatgcc gttactggca ccgcttggcg
     8521 atctggtcgg tgttaaccgt caggttaccg tgctggcgtt ccagtttggt gatggcttca
     8581 gccacatcat ttacccaacc tcagcttcgt taatggcaac gctcggtgtg tgccgggtgg
     8641 acttccgtaa ctggctgaag gtgggcgcga ccctgcttgg actgctgttt attatgtcca
     8701 gcgtcgtagt gatcggcgct cagttgatgg gctaccacta aaaatgttaa gagccgcaat
     8761 tgcggctctt ttttcattct gccgtttcag tctctacggc ttcatttttg gcattgcgct
     8821 tcatccacaa cgccagcgct ttcagcgagt ctggagtgaa ctcatcgcag cgtgcggtga
     8881 tttcttccgg cgtcagccag caaactccac tgacttcatc ttcctgtagg gcgaagggac
     8941 cgtgagagac gcaactgaac aacgcgcccc agacacggca atttttatct tcgaaataga
     9001 actgcccgtg ctcggcaaag gggacaccgg caatgcccaa ctcttcttcc gcttcgcgac
     9061 gcgcggattc cagcagttgc tcatcggcct gaactacacc gcctgcggtc gcatctaaca
     9121 tgccgggtaa aaagtctttt gtctcggtac gacgctggac cagaattttg cccatgccat
     9181 catgcacgac gatgtaagtt gcacgatgac gcagacactg tgcccgcatt tgttcccggc
     9241 tggcttgtgc aatgacttcg ttctcttcat tgacaatatc cacccattca gtacttgcca
     9301 aacgacgctg ttccattatc aggaaacctt ctttttctgg cgctcttacg gcgcattttg
     9361 agttgtgggt aaattacgga ttaatcgcga cctgcgcaat gatactttga tcattgagtg
     9421 cgattacact aagtacgtca ttatccagca tgccataacc cgccggatta ccgcctttcg
     9481 gaatactcac cgagccgggg ttgaagtgga aaatctcccc ccgctgttct gccactggca
     9541 gatgggtatg accgtacacc agcacatcgt tctggtttaa agcaggtaga ttttccgggc
     9601 caaaaagatg accatgcgtc aaaaacagac gttgtttttc cagtaatacc tgttgccacg
     9661 gcgcggttat cgggaaatgc agcatcattt gatccacttc gctgtcgcag ttgccacgca
     9721 cagcgataac cttatgtgcc acttcattaa gccgttcagc gactttggct ggcgcgtaac
     9781 cctccggtaa agcattacgc gggccgtgat tcaacacgtc gccaaggatc accagccact
     9841 gggcaccgct ttggacaaac aactccacaa cacgttccgt cgccggtaac gacccatgaa
     9901 tgtccgatgc aaacatcagt ttcatcactc actcctcgtc gaaaaaacgt aagctatgat
     9961 actggattct gtcgttgcta tcagccagat tgcttcgccg aaagtgcaat aaaacgctgg
    10021 acagacgctc gctgccactg gaatgtcgca taatccacca ggcgttctgg cacctcatcg
    10081 ccatgtagta ccaggcggtt aatcattagc gccagatcag tatcagcaat gcaccattca
    10141 ccaaataaat tcggctgacc cagtgctaac aaatgttctg ccatcgcgaa cagtttctct
    10201 gcactggctt ttccctcggc cgttagtggc gcttttttcg cccccgcaaa gacaacatcc
    10261 gtcggacgct cttcgcggat gggcatcaga tcgctgcgca gccaggcctg aatctgtcgt
    10321 gcacgcgcac gattttctaa atcaagcgga taaatacgtt cccaggtcgg tggcgcaaat
    10381 cgatcttcca gatactccgc aatggcagaa gattcactca actcaaaatc atcgatttgt
    10441 aataacggca cacggcgtgt ctgaccgtaa ccttgccacg tcggctgcaa atgttcaccg
    10501 ctgtcgaggt cgatggtctt gatatgaaac gacaggcctt tttcctgcaa cgccacccag
    10561 gcggataaca cataagggga gaaaaagtgg gcatctgacc aaagcgtgat agcgggttta
    10621 ctcattatgt cctcggcaat aaagggggct ggctatcaaa ctatagagcc tttttgtcac
    10681 tgtcacgtga cgaatactca cgtaagtcgc cgcgctttct atacttgtgg tgttcagatg
    10741 attaaacaac cggagctgca atgatcgatc tctatttcgc cccgacaccc aatggtcaca
    10801 aaattacgct gtttctcgaa gaagcagagc ttgattatcg cttgattaag gtagacttgg
    10861 ggaaaggcgg tcagtttcgt ccggaatttt tgcgcatttc gcccaacaac aaaattccgg
    10921 caattgttga tcattctcct gccgatggcg gcgaaccgtt aagcctcttt gaggctgctg
    10981 ccattttgtt gtatctggct gagaaaacag gactcttttt gagtcatgaa acgcgtgagc
    11041 gcgccgccac attacagtgg ttattctggc aggtaggcgg actggggccg atgcttgggc
    11101 aaaatcatca ttttaatcac gcagcccccc aaaccattcc ttacgctatt gaacgttatc
    11161 aggttgaaac tcagcgtctt taccatgtac taaacaagcg gctggaaaac tcgccctggc
    11221 tgggaggcga gaactacagc attgcggata ttgcctgctg gccgtgggtt aatgcctgga
    11281 ctcgcctgcg aattgacctc gcaatgtatc cggcagtcaa gaactggcat gagcggatcc
    11341 gttcgcgccc tgccaccggg caggcactgc taaaagcaca actcggtgat gagcgttcgg
    11401 atagttaaca gaaacaggtt ctcgtgtatt atttcatcct aagtaaaaca acggagaacc
    11461 tgcaatggca caacctgccg ctattattcg tataaagaac cttcgtttgc gtacgtttat
    11521 cggaattaag gaagaagaaa ttaacaaccg tcaggatatt gttatcaatg tgacgatcca
    11581 ctaccccgcc gataaagcgc gcactagcga agatatcaac gatgcgctga attatcgcac
    11641 cgtaacgaaa aacattattc agcatgtaga gaataaccgt ttctctttgc tggaaaaatt
    11701 aactcaggat gtgctcgata tcgcacgtga acatcactgg gtgacgtatg ctgaagtgga
    11761 gatcgataaa ctgcacgcgc tgcgctacgc cgattcggta tccatgacct taagctggca
    11821 gcgttaatcg ccatatcggg aggctgcatg aacatagtga tcaccggagg gacgggatta
    11881 attggtcgcc atttgattcc gcgtttgctg gagctgggcc atcaaattac ggtagtgacg
    11941 cgtaacccgc agaaagccag ttccgttctc ggccctcggg tgacactatg gcaagggctt
    12001 gccgatcaaa gcaacctcaa cggcgttgat gcggtaatca acctggccgg agaaccgatt
    12061 gctgataaac gctggactca caagcaaaaa gagcgtctct gccaaagccg ctggaatatc
    12121 acgcaaaaac tggtcgattt gattaatgct agcgacacgc cgccgtcggt actcatttcc
    12181 ggctcggcaa cgggctatta tggcgactta ggtgaagtgg tggttaccga agaggaaccg
    12241 ccgcataacg aatttactca taaactctgc gcccgctggg aagagattgc ctgccgggcg
    12301 caaagtgaca aaacgcgagt gtgcctgctg cgaaccggcg tggtactggc accggacggc
    12361 ggtattctcg gtaaaatgct gccaccgttt cgtcttggcc tgggcgggcc gattggttcc
    12421 ggtcggcagt atctggcctg gattcatatt gatgatatgg tcaacggcat tctctggctg
    12481 ctggataacg agctgcgcgg gccatttaat atggtttcgc cctacccggt acgcaatgaa
    12541 caatttgccc atgcgctcgg tcatgcgctg catcgcccgg ctattttgcg cgtccccaca
    12601 accgccattc ggctgttaat gggcgaatct tcagtactgg tattaggtgg acaacgcgcg
    12661 ctgcctaaac ggctggaaga agcgggtttt gcgtttcgct ggtacgattt agaagaggcg
    12721 ctggcggatg tcgttcgctg atgtggttta cagcaaacat ccgccagtta actccctgtg
    12781 ttacaggatt agtggctttg cgcgataaga tcgtctggtg aaagtcgggt caccatcata
    12841 actaactctc tgtctaaacc tctatccagc atctcctgag caatacgcag ggcttcttcg
    12901 tgtttgccct gcattgcgcc ttcttcacgt aatctgtcag caatggtcat cagtttctcc
    12961 ttttcttgtg gtgcgcgttc tgttatctca ccaataaatg cacggaaacg ctgggcatcc
    13021 cctgtttgta atacgtaatt aaacagggct tttagctgtc tgtcattagt gttccctgta
    13081 actagcagcg aaacaatttg gtcgactaat cccaacagat cgcgctgacg aatatgtttc
    13141 tgaattaact ccaacagcgc cattttgcgg tgttgcataa tctcgtcatc cggcaccacg
    13201 gtaatatcca ccaacggaaa cgctgatgaa tatattttgc gggctatagc aggctcggca
    13261 aattcatcaa gccagcagag tgaataagga taaggacttc tgcaaccatg ataaaacagc
    13321 atcgggatca ccaatggaag ctctttatag cccgcatcaa gatggttttg cattgccgca
    13381 atggaataac gcatcatgcg aaaagccatt aattcttccg gcttactttg gtgctctatc
    13441 actacataaa tataacccac tccctcctgc gttttcacag accacaagag gtcggaataa
    13501 tattgccgca ggtcttcatc aataaaactg tttggttcta gtttaagcgt cgttaaatca
    13561 cacagtttgc gcagcggcgc gggaagatga atattaataa aattccgcgc ggtgtctgga
    13621 tggcgtaaaa aagatttaaa taccgcatca tgcggcgtgg aagttgtcga tattgtcatg
    13681 gcgttccgtc acccctcaaa tttaagatga cgcgacaata accacgatta acacccaatg
    13741 cagcaggcag tttttatctt tggacagcga attcagaagt atttttagcg gactgcaact
    13801 tttacatttc agcggaagct gactcgcaaa ttccccttga aaatactccg gcagcgttag
    13861 ccaccggaga ggaaattatt tcagcgatcc cttaaggaac tgttgcagac gagggctttg
    13921 cgggttgcca aataactgct ccggcgcgcc ctcttcttct attttcccct gatggaggaa
    13981 aatgacgtga gtagaaacat ggcgagcaaa gcccatttcg tgagtcacta ccaccatggt
    14041 tttcccctct tctgccagtt gctgcataat acgtaacact tcgcctacca gttcaggatc
    14101 gagcgccgag gtaggttcat caaacagtaa aacttctggt tccatcgcca gcgcccgcgc
    14161 gatagaaaca cgctgttgct gaccgccgga aagatgcacc ggatatttcc cctgcgcacg
    14221 ttcgtctatc ccgactttcg ccagatactt caccgcccgc tcgcgcgctt cctgcttgct
    14281 caggcccaac acctgaatcg gcgcttccat gacgttttcc aacaccgtca tatggctcca
    14341 gagattgaag tgctggaata ccatcgtcag gcgtgtgcgc agtaagcgca gttgattttt
    14401 atcggcgact ttgagttgac cgtctttgtc gcgcaccaga ttgatcgtct ggccattgac
    14461 cacgatcgat ccttcactcg gtttttcgag gaagttaatg cagcgcagaa aggtactttt
    14521 ccccgatccc gacgatccga tgatgcttat tacatctccg gcattcgctt gcagtgagac
    14581 ccctttcagc acttcgtgtt cgccatagcg tttgtgcaaa tcgataacgt ttaatttatt
    14641 ctcggacatc gtattctcag tgcgttgaag aaggtttcac atgctgcaac cagcgttttt
    14701 ccgctctgcg aaagaggctg atcaggacat aagagatgat taaatagagc accgcggcaa
    14761 tgccgaaggc ggtaaaaggt tgatacgtgg cggcgttaat atcgcgggct attttcagca
    14821 gatccggcac agtggccgta aacgccagcg cagtagagtg cagcatcagg atcacttcgt
    14881 tgctgtatgc cggtaacgca atacgcagcg cagaaggcaa aataatgcag cgatacattt
    14941 taaaggtcga gaagccatag gctctggcgg cttcaatttc cccatgcgga accgaacgga
    15001 ttgccccagc aaaaatctcg gtagtgtaag cgcaggtgtt aagcgtcagc gccagcacgg
    15061 tacagttcag gccactgcgg aagaaagcgt taaggaattc ggttccctta acaatctcaa
    15121 gcgtgtacat gccggaatag aacaccagca actgaacata cagcggcgta ccgcgaaaaa
    15181 tataggtaaa taaccagatt ggaaactgga tgtatttatt actggagaca cgaccaatcg
    15241 ccagaaacag cgccaggact ccgcctatca ctaccgacaa aataagcagc cacagagtga
    15301 tcgccacacc agtaaagcga taaccgtcgg tccacagcag cggtttccag tattcatgta
    15361 agatttcgat cacaggtcag ccctcttcac acccacggag tagcggcgct caaggaacag
    15421 cagcacacca ttggaaacgg tggtgaaaac caggtaaatc acgccacaga cgatggcgaa
    15481 atagaacggt tcccaggtac ttttgcctgc cagctgcgtg gctttgacca catcttccag
    15541 gccgagtaac gaaaccagtg cggtagattt gaggatcacc tgccagttgt tgccaatgcc
    15601 tggcagggcg taacgcatca tcgccggaaa catgatccgc cgaaacactt gcccgcgagt
    15661 aaaaccgaac gccgtcgccg cctctatatg ccctttcggc actgccataa acgcgccacg
    15721 gaacgtttcg gtaaaataag caccgtagat aaaaccgaga gtgataatac cagcgaccat
    15781 cggatcgata tcaatctgcc cgacgcccat cgcctccgtc accatgttta gcgcaatctg
    15841 caaaccatag aaaatcagca gcatcaacac caagtccggc acgcctcgaa tcagcgtggt
    15901 gtacccttcg aaaatcagcc ccgaaagccg attttgcgag agcttaccgc cagcgccaat
    15961 taaaccgatg attacagcga gcactacaga gctgatagcc agctccagcg tgacgagcgc
    16021 accctgtaaa ataacacctg aaaacccata caacatgctg cctgtcctgt cgtgtgtggt
    16081 gaattaccgc ctcttgtctc cctccggtta cccgaaggga gcgggctttc tttcaccgat
    16141 ggcgattaac caccataaac atcaaaatcg aagtactttt tcgctaattt ctcgtaagta
    16201 ccgtcagcgc gcatttcggc aaaggctttg ttcagtgcct cgcgcagttc gttatcttct
    16261 ttacgcaggc ccatgccggt ccctacgcca aacagttttt catctttaac agacggacca
    16321 ccgaatttgt aatctttacc gacgggttgt ttgaggaa
//
LOCUS       DAZSNM010000082        16294 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZSNM010000082 DAZSNM010000000
VERSION     DAZSNM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16294
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.082"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..617
                     /locus_tag="ACHW42_002189"
                     /pseudo
     CDS             1..617
                     /locus_tag="ACHW42_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            618..2267
                     /locus_tag="ACHW42_002190"
     CDS             618..2267
                     /locus_tag="ACHW42_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR8944005.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            2272..3048
                     /locus_tag="ACHW42_002191"
     CDS             2272..3048
                     /locus_tag="ACHW42_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR8944006.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            3323..6172
                     /gene="rcsC"
                     /locus_tag="ACHW42_002192"
     CDS             3323..6172
                     /gene="rcsC"
                     /locus_tag="ACHW42_002192"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR8944007.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(6372..7022)
                     /gene="rcsB"
                     /locus_tag="ACHW42_002193"
     CDS             complement(6372..7022)
                     /gene="rcsB"
                     /locus_tag="ACHW42_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR8944008.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(7039..9711)
                     /gene="rcsD"
                     /locus_tag="ACHW42_002194"
     CDS             complement(7039..9711)
                     /gene="rcsD"
                     /locus_tag="ACHW42_002194"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR8944009.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            10449..11570
                     /gene="ompC"
                     /locus_tag="ACHW42_002195"
     CDS             10449..11570
                     /gene="ompC"
                     /locus_tag="ACHW42_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR8944010.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            11682..12737
                     /gene="apbE"
                     /locus_tag="ACHW42_002196"
     CDS             11682..12737
                     /gene="apbE"
                     /locus_tag="ACHW42_002196"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR8944011.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            12811..13875
                     /gene="ada"
                     /locus_tag="ACHW42_002197"
     CDS             12811..13875
                     /gene="ada"
                     /locus_tag="ACHW42_002197"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR8944012.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            13875..14525
                     /gene="alkB"
                     /locus_tag="ACHW42_002198"
     CDS             13875..14525
                     /gene="alkB"
                     /locus_tag="ACHW42_002198"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR8944013.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            14601..16244
                     /gene="yojI"
                     /locus_tag="ACHW42_002199"
     CDS             14601..16244
                     /gene="yojI"
                     /locus_tag="ACHW42_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR8944014.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         3929 a         3957 c         4345 g         4063 t
ORIGIN      
        1 tgggctaaac ataagttaac tggagggggc gaagactggc gttgggttgg ttagggtgtg
       61 ccggacattc tctcttttgg tgacgaatta tcgccgcaaa tgtgcaggtc cgctggcgtg
      121 aaccggcaaa aacggctcaa caaagtaaca ttccggtgac cgttgaacgc cagttgtatc
      181 ggcttatccc cggtgaagaa gagatgagct ttactctgca gccggtgacc agcaatgaga
      241 ttgacagcga tgcgctgtat ctcgatgaaa ttacgcttac cagcgagcag gatgcagttc
      301 tgcgctatgg tcaggtggaa gtaccgctcc cgccgggagc tgacgttgag cgcacaacat
      361 ggggcatttc agtcaataaa cccaacgccg cgaaacagca ggggcaattg ctggaaaaag
      421 cgcgtaatga aatgggcgaa ctggcctata tggtgccggt gaaagaactg acgggaacgg
      481 tcactttccg ccatttgctg cgcttctcgc aaaaagggca attcgttctg cctcctgctc
      541 gttatgtgcg ttcctatgca cctgcgcagc aaagtgtcgc ggcagggagt gaatggaccg
      601 ggatgcaggt gaaataagtg aactggcgca gaatcgtctg gctattggcg ttggttactc
      661 tgccaacgct tgcggaggag acgccgttac aactggtgct gcgcggtgcg cagcacgatc
      721 aactgtatca gctgtcgtca tcaggcgtga ccaaagtctc agcattacct gactcgctca
      781 ccacgccgtt agggagtttg tggaaactgt atgtttacgc ctggctggag gatacccatc
      841 aaccggagca gccctaccag tgtcgcggaa actccccgga agaggtttat tgttgtcagg
      901 ctggggagag cataacgcgc gataccgcgc tggtacgttc ctgtgggctc tattttgcgc
      961 cgcagcggtt gcatattggc gcagatgtgt gggggcaata ctggcaacag cgtcaggcac
     1021 ctgcgtggtt agcgtctctt acaacgctga agccagaaac ctcggtaacg gttaaatcgc
     1081 tgcttgattc attagcgacg ttacctgcgc aaaacaaagc tcaggaagtc ttactggatg
     1141 tggtgctgga cgaggcgaaa atcggtgtcg catccatgct cggtagtcgg gtacgggtga
     1201 aaacctggag ctggtttgcc gatgataaac aggaaatccg tcagggtggt tttgctggct
     1261 ggttaaccga tggtacaccg ctgtgggtta ctggcagtgg caccagcaaa acggtactaa
     1321 cccgttatgc gacggtgctt aatcgggttt tgccagtgcc gacgcaggtt gccagtgggc
     1381 aatgtgtaga ggtggagctg tttgcccgct atccgctgaa gaaaatcaca gcggaaaaga
     1441 gtacgacggc agtaaaaccc agcgtactta atgggcgcta tcgtgtgacg tttactaatg
     1501 gtaaccacat tacgtttgtc agccacggtg agacgacctt actgagcgaa aagggcaaac
     1561 tgaaattgca gtcgcatctc gatcgtgaag agtacgttgc cagagttctg gatcgcgagg
     1621 cgaaaagtac gccacctgag gcagcgaaag ccatgactgt ggcgatacgt acttttttac
     1681 agcaaaacgc caatcgggaa ggtgattgcc tgaccatccc cgacagcagc gccacgcagc
     1741 gtgtttctgc ttcgccagcg accacggggg cgcgaacgat gaccgcctgg acgcaggagc
     1801 tcatctatgc aggcgatccg gttcattatc atggcagtcg cgctaccgaa gggacacttt
     1861 cctggcggca agccacggcc caggcggggc agggggagcg ttacgaccag atactcgcgt
     1921 ttgcttatcc cgacaacagc cttagccgct ggggagctcc acgctcaacc tgccagttat
     1981 tacccaaagc gaaagcctgg ctggcgaaaa aaatgccgca gtggcggcgt atattacaag
     2041 gcgagacggg gtacaacgaa ccagacgtgt ttgcggtctg tcgtttagtc tcaggtttcc
     2101 cctataccga tcggcagcag aaacggctgt ttattcgtaa cttctttacg cttcaggatc
     2161 ggctggattt aacccatgag tatctgcact tggcctttga tggttatccc accgggcttg
     2221 atgagaacta tatcgaaacg ctgacccgac aattattaat ggactaatgc tatgcgaaag
     2281 atttttcttc cgttgttact ggtggcactt tcgcctgtcg ctcacagtga aggtgtgcag
     2341 gaagtcgaga ttgacgcacc gctctctggc tggcatccgg cagagggcga agatgccagt
     2401 ttttcacaat cgatcaatta cccggcgtcg tccgtcaaca tggcagatga tcaaaatatc
     2461 tcagcgcaga tccgcggcaa aataaagaat tatgctgcgg cgggtaaagt tcagcagggc
     2521 cggctggtgg tcaacggtgc cagtatgccg cagagaattg aatccgatgg ttcatttgca
     2581 cgcccttata ttttcactga aggcagcaac agcgtgcagg tcatcagccc ggatgggcaa
     2641 agccggcaaa aaatgcagtt ttactcaacg ccgggtacag gaactattcg tgcacgttta
     2701 cggctggttc tctcgtggga tacggacaat accgatctcg atctccatgt tgtcacgccc
     2761 gatggcgaac acgcctggta cggtaacacc gtgctgaaaa acagtggtgc actggatatg
     2821 gatgtcacga cggggtacgg acccgagatt ttcgccatgc cagcaccagt tcacgggcgc
     2881 tatcaggtgt atatcaacta ctatggtggg cgcagcgaaa cggaattgac gaccgcccaa
     2941 ctgacgctga tcaccgatga aggatcggtc aatgagaaac aggaaacatt tattgtaccg
     3001 atgcgtaatg ctggcgagct gacgctggtg aaaagttttg actggtgatc catcccttca
     3061 cacaacctgg ctctctcttt tttcagataa gactgctggg cgatctgccg acgccaggat
     3121 ataaccatta acaagcgtcg gcagattggt catttcttat ttttcgtact gagaactgtc
     3181 catctactcc ggctttcctg gttaaggcaa tggtttctaa gcgatacgca ttattgcaat
     3241 ggtgacagaa cattgcggta acacgctttt accgctacct taaccacact ccatcggtca
     3301 cctgaggcgg agcttctccc ctttgaaata ccttgcttct tttcgtacaa ccctgaaagc
     3361 ctcgcgctac atgttcagag cattggcgtt agtgctctgg ctgttgattg ctttttcatc
     3421 cgttttttac atcgttaatg cgttacatca gcgagaatcg gaaattcgtc aggaatttaa
     3481 tctgagttcc gatcaggctc agcgctttat tcaacgcacc tctgatgtga tgaaagagct
     3541 taagtacatc gccgaaaatc gcttatcggc agaaaacggt gtgctttccc cgcgtggacg
     3601 agaaacgcag gcggatgtgc ctgcgtttga accgctgttt gccgactccg attgttccgc
     3661 aatgagtaac acctggcgag gttctctgga gtcattggcg tggtttatgc gctactggcg
     3721 cgataatttt tctgcggctt acgatctcaa ccgggtattt ttaatcggca gtgataacct
     3781 ctgcatggcc aatttcggtc tgcgtgatat gccagtggaa cgcgataccg cgttgaaagc
     3841 gttgcatgaa cgcatcaata aatatcgaaa tgcaccacaa gatgatagcg gcagtaacct
     3901 ctactggatc agcgaaggtc cgcgcccttg cgtcgggtat ttttacgcgt tgacgccagt
     3961 ttatctggcg aaccggttgc aggcgctttt gggtgtcgag cagaccatcc ggatggagaa
     4021 ctttttctta ccgggtacgt tgccgatggg ggttaccatt cttgatgaaa atggtcatac
     4081 cctgatttcg cttaccggac cagaaagtaa aattaagggc gatcctcgct ggatgcagga
     4141 acgctcctgg tttggctata cggaagggtt ccgggagctg gtgctgaaga aaaatctgcc
     4201 accctcatcg ctaagcatcg tgtattcggt gccggttgat aaggtgctgg aacgcattcg
     4261 catgttgatc cttaacgcaa ttttgctgaa tgtgcttgcc ggagctgcat tgtttactct
     4321 cgcgcggatg tacgagcgac gtattttcat tccggcggaa agcgacgccc tgcgactgga
     4381 agaacatgag cagttcaatc gcaagattgt cgcctccgcg ccagtgggta tctgcatttt
     4441 gcgtaccgct gatggcgtca atattttaag taacgaactg gcgcatacct atctcaatat
     4501 gcttacgcat gaggaccgcc aacgactgac gcaaattatc tgtgggcagc aggtcaattt
     4561 tgttgatgtc ctgaccagca acaataccaa tctgcaaatc agcttcgtcc attcgcgcta
     4621 tcgtaatgaa aacgtggcca tttgtgtgct ggtggatgtt tcttcgcgcg tgaagatgga
     4681 agagtcgttg caggagatgg cacaagcagc ggaacaggcg agccagtcaa aatcgatgtt
     4741 ccttgccacc gtcagtcatg agctgcgaac gccgctgtat ggcattatcg gtaacctgga
     4801 tctgttgcaa accaaagagt taccgaaagg cgtcgatcgt ctggtgacgg caatgaacaa
     4861 ctcttccagc ctgttgttga aaattatcag cgatattctc gatttctcga agattgaatc
     4921 ggaacagttg aagatcgaac cgcgtgagtt ttcaccgcgt gaagtgatga accacatcac
     4981 cgccaactat ttaccgctgg tggtacgcaa gcagttaggc ttgtactgct ttattgaacc
     5041 ggatgtgcca gtggccttaa atggcgaccc gatgcgttta cagcaggtca tctccaacct
     5101 gttgagtaac gccataaaat tcaccgatac cggctgtata gttttgcatg ttcgcgctga
     5161 tggcgattat ctctctatcc gtgttcgcga taccggcgtg gggattccgg cgaaagaagt
     5221 ggtgcgcttg tttgatccct tcttccaggt tggaacgggc gtacagcgaa acttccaggg
     5281 gaccggtctg ggtctggcga tttgtgaaaa actgatcagc atgatggacg gcgatatctc
     5341 ggtagattca gaaccgggaa tgggcagcca gtttaccgtg cgtattccgt tgtacggcgc
     5401 tcagtacccg cagaaaaaag gcgtggaagg gttgagtggt aaacgctgct ggctggcggt
     5461 ccgcaatgcg tcgctctgtc agttcctgga aaccagtttg cagcgcagcg gcatcgtcgt
     5521 tacaacatac gaagggcagg aaccgactcc cgaagatgtg ttaatcactg acgaggtagt
     5581 gagtaaaaaa tggcagggca gagcggtagt gaccttctgt cgtcgccata ttggtattcc
     5641 gctggagaaa gcgccagggg agtgggtaca cagtgtggct gctccgcatg agctaccggc
     5701 attgttggcg cgtatttatt tgatcgagat ggagagcgac gatcctgcta acgctctgcc
     5761 gtcgacggac aaagcggtca gcgataacga cgatatgatg attctggtcg tggatgatca
     5821 tccgattaac cgacgtctgc tggcagatca gttgggatcg ttgggctatc aatgtaaaac
     5881 cgcgaatgat ggcgtcgatg cgcttaatgt acttagtaag aatcatattg atatcgtgct
     5941 tagcgacgtc aacatgccaa atatggatgg ttaccgcttg acgcaacgca ttcgtcagtt
     6001 gggactgacg ttgccggtaa tcggagtaac tgctaatgcg ttggctgaag agaagcagcg
     6061 gtgtctggag tccggtatgg acagctgcct ctcgaagccg gtaacgctgg atgtgataaa
     6121 acagacgctg acggtatatg ccgagagggt caggaaatcg cgggattcgt aggacggata
     6181 aggcgtttac gccgcatccg gcattcagtg caaatgccag ataagacact gacgcgtctt
     6241 atctggccta ctttaatgta atctgttgaa ataatgggaa tcgtaggccg gataaggcgt
     6301 ttacgccgca tccggcaatc ggtgcaaatg ccagataaga cactaacgcg tcttatctgg
     6361 cctacagatg attagtcttt atctgccgga cttaaggtca cagaagagag ataattcagc
     6421 agggcgatat cgttctcgac gcccagcttc atcatcgcag atttcttctg gctactgatg
     6481 gttttaatac tgcggttcag ctttttagcg atctcggtca ctaggaagcc ttccgcaaac
     6541 aggcgcagaa cttcactctc ttttggcgag agacgcttgt caccgtaacc accagcactg
     6601 attttttcca acaggcgaga aacgctttcc ggggtaaatt tcttcccttt ctgcagcgcg
     6661 gcgagagctt tcggcagatc ggtcggtgca ccttgtttca gcacgatccc ttcgatatcc
     6721 agatccaata ccgcactaag aatcgccggg ttgttgttca tagtcagaac aatgatcgac
     6781 aggcttggga aatggcgctt gatgtacttg attaaggtaa tgccatcgcc gtacttatcg
     6841 ccaggcatgg agagatcggt aatcaacaca tgcgcatcca gtttcggcag gttgttgatc
     6901 agtgctgtag agtcttcaaa ttcgccgaca acattcaccc actcaatttg ctcaagtgat
     6961 ttgcgaatac cgaacaagac tatcggatgg tcatcggcaa taattacgtt catattgttc
     7021 atgtaatagg ctaccttgct acagcaagct cttgacataa ctgtcaatgt cgctgatgta
     7081 tttttctatt cctggaacat ccttctcacg aatcagatgt tccagcgttt cacataactg
     7141 cttgccgggt accagattta gcatggcaaa tacgccttta agacgatgag ccgtttgggc
     7201 taacgcagca aagtcactgg ttgctgcttc agtatacagc ctcttaacat catccggtac
     7261 tgtgtctaca aagagcgcat aatagccgct ggcatggagt tgcgcatttt catctccgcc
     7321 cagaggcgat tctgtcacct cttcctgtgc cagttgcact tcaattaatt gtaagaccgc
     7381 ttcctgcata gcgttgctca tattgaagtt gacgcacaat tgaccaggcc caatttctcg
     7441 tacgccagac tcatcatcgc ttaaaagcaa gccagaggca gtaagattag acggattatc
     7501 cgttaaaaag atatcataat cttgactaat taatctttca tcgggtgtga tacaggttgc
     7561 accccaattt tctaacaggc gagtgacaat attccgaatt tctgccgaag taacatccac
     7621 cattacgcag acatcatcca gtaaacgctc ttcttcctct tcaacttccg ggtcagcggc
     7681 gagcattttg atatgcacag agtagcgtgt accaagccca tcccgcgttt tgatgtttaa
     7741 atgaccgccc agtttacgtg ccagttgatc gctcagccag aatgccagcg ggtccgcctt
     7801 tccataacga tcgttttggg tctggttgat aaacgggaag tgcaaattat ccatttcatg
     7861 aatacttacg ccttctcccg tgtccagaat gcggaatgtc aggcggtctt cagaggactc
     7921 atcctgatca acctcaaggg tgatttttcc caattgcgtt gaggtcacgg catattgcat
     7981 cagtagcagc aaaatacgtc gtaaggcatc gcgatcgccg cggcgcatat cgtgtgcttt
     8041 cagatgattg ttaatcagca gttgcagacc tttacgcttg atggcaggca acactgaagg
     8101 cacaacttca tcaattaaat cctgcacgga gaacagcacc gtctcacttt tccagctatc
     8161 atccgcaagc atgttcgcta actgtatttc ttcgaccagc cgcaccagca catctgcctg
     8221 attcgccagt tgtttgcttt ccggggcgtt gagtttagcc gcactctccg ccagggactg
     8281 tgcgggttct ttcagcgcat cgccaatgtt tttcataaag gtcatccgcc cctgctggtt
     8341 tttctcatac agacgctgcg cctgcttgag tttcttgttt accagcactt cgcgatcctg
     8401 atcgcgaata atgaaaattt gtgtgcgcgg cgcgacctgg ctgcggaaca tgcggatctc
     8461 atacagctcg ttattgatcg tcgcctgaat aatcccctga tgctgttccg ccatggtggt
     8521 gatgttttgc agattcagat gcggcagcaa atgatcggca attttgttac ttatgacagt
     8581 gcggttcgat tcctgatcgt gaaccagcag gccgagcggc aacagtgaga ctatctcttc
     8641 attgattgcc cgtaaaatgc gcaattcgtt attgaccgcc gtgctgggga cgctttctgt
     8701 actgcggctg gagaaatggc ggaatgtggt atagccaaat aacgccagcg ccagcaaacc
     8761 gatgttcagc agcagtggca gcagaatgtt ttgcaacgta tccagcaata aggtgccata
     8821 aggaacctgc cagaccaggc gcatatcggt agagttgagt gccgaggaga tttcaatctt
     8881 cgtactgtta aagtggatac tgacgctatc cgtcccttct ttctcattat cattgtttcc
     8941 cgttgccgtc gcgtctggct caaggcggaa actgtccagc ggcatacccg gtgggatcaa
     9001 atcattaatc ggcagatcaa aagccacgac cgttgccaga tgtcccggct ggttgaacgt
     9061 ggtacgcaag gtaaagtaat gaccgttctg ccaggctagg cggcgcatgt tagaaaaact
     9121 ttcgcgttca tcgagggcgt tggcctgttg taacatttct gcacgacgtg aatcaacaat
     9181 gtcactgacg gtcgattctt taaatccgga ggtgagatct ttaaggggta gggttgagat
     9241 cagcaccaga ctgttatcct gaccattcag gtaatacatc gaccacggta cattttctgc
     9301 accccacaat gtatccagat aagtggacat ccgctgagtc atctcaagcg ttgagttgtc
     9361 gtgagagcca aagattaacg cttccgtttt gcggcgtggt ttctccagat agtagacatc
     9421 ctgtttcagg cgcgtctctt gtaaaccttc gccggaggag ggggaggtcg tcgcggcaat
     9481 gttgtcgtag atctgcctgg tcacgtaacg ccaggtatcg acgcgctttt ggattgcgtg
     9541 ggtaatgtcg acaatctggt aacttttatc tttcagccaa gcgttaacgg cactttgtac
     9601 cattaccccc atcgtcacca gtaacacaat gatcaacagt aaaaagaagc gggtaatgct
     9661 ccccggtagg agtgaaaagc gggtcgtggc cgttgtctct ttctgacgca ttcgtgttta
     9721 tgacctgtta aaacttcgcg aagtgatagg gcagtataaa gggtacagtg tgaaattcca
     9781 gactcttacc tgattgatga ttctctattc tcccgtgata tttatcatcc tgtatggggg
     9841 gaaatagatg tacaagcgcc attttgctgt acaaataacc tacaaaaagc ccaacgaaat
     9901 taattgcaaa taaacaaaga ttgctggaaa ttatgctgat gttaattatt tgtgaaatag
     9961 ttaacaagcg ttatagtttt tctgtggtag cacagaataa tgaaaagtgt gtaaagaagg
    10021 gtaaaaaaac cgaatgcgag gcatccggtt gaaatagggg taaacagaca ttcagaaatg
    10081 aatgacggta ataaataaag ttaatgatga tagcgggagt tattctagtt gcgagtgaag
    10141 gttttgtttt gacattcagt gctgtcaaat acttaagaat aagttattga ttttaacctt
    10201 gaattattat tgcttgatgt taggtgctta tttcgccatt ccgcaataat cttaaaaagt
    10261 tcccttgtat ttacattttg aaacgtctat agcgataaat gaaacatctt aaaagtttta
    10321 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggacttg ccgactgatt
    10381 aatgagggtt aatcagtatg cagtggcata aaaaagcaaa taaaggcata taacagaggg
    10441 ttaataacat gaaagttaaa gtactgtccc tcctggtccc agctctgctg gtagcaggcg
    10501 cagcaaacgc tgctgaagtt tacaacaaag acggcaacaa attagatctg tacggtaaag
    10561 tagacggcct gcactatttc tctgacgaca agtctgtaga tggcgaccag acctacatgc
    10621 gtcttggctt caaaggtgaa actcaggtta ctgaccagct gaccggttac ggccagtggg
    10681 aatatcagat ccagggcaac agcgctgaaa acgaaaacaa ctcctggacc cgtgtggcat
    10741 tcgcaggtct gaaattccag gatgtaggtt ctttcgacta cggtcgtaac tacggcgtag
    10801 tttacgacgt aacttcctgg accgacgtac tgccagaatt cggtggtgac acttacggtt
    10861 ctgacaactt catgcagcag cgtggtaacg gcttcgcaac ctaccgtagc accgacttct
    10921 ttggtctggt tgacggtctg aacttcgctg ttcagtatca gggcaaaaac ggtagcccgg
    10981 aaggtgaagg catgaccaac aacggtcgtg aagctctgcg tcagaacggt gacggcgttg
    11041 gcggttctat tacttatgat tacgaaggct tcggtatcgg tgctgcagtt tccagctcca
    11101 aacgtactga tgaccagaac ttcggtctga accgttatga tgagcgttac atcggtaatg
    11161 gcgaccgcgc tgaaacctac accggtggtc tgaaatacga cgcgaacaac atctacctag
    11221 ctgctcagta cacccagacc tacaacgcaa ctcgcgtagg caacttgggt tgggcgaaca
    11281 aagcacagaa cttcgaagct gttgctcagt accagttcga cttcggtctg cgtccgtccc
    11341 tggcttacct gcagtctaaa ggtaaaaacc tgggtgtcat caatggtcgt aactacgacg
    11401 acgaagatat cctgaaatat gttgatgttg gtgcgaccta ctacttcaac aaaaacatgt
    11461 ccacctacgt tgactacaaa atcaacctgc tggacgacaa ccagttcact cgtgacgctg
    11521 gcatcaacac tgataacatc gtagctctgg gtctggttta ccagttctaa tctcgatgga
    11581 tatcgaacaa agggcctgcg ggcccttttt tcattgtttt cagcgtacaa acacagtttt
    11641 ttggtgtact cttgcgaccg ttcgcatgag gataatcacg tatggaaata agctttaccc
    11701 gcgtggcact gctggttgcc gcgctcttct ttgttggttg cgatcaaaaa ccacaacccg
    11761 ccaaaaccca cgctactgaa gttaccgttc ttgaaggcaa aaccatgggt accttctggc
    11821 gtgccagcat cccgggcatt gatgccaaac gcagtgccga acttaaagaa aagattcaga
    11881 cccagctgga cgccgacgat cagctgcttt cgacctataa aaaagattcc gcgctgatgc
    11941 gctttaacga ctcgcaaagt ttgtcgccgt ggccggtaag tgaagcgatg gccgatatcg
    12001 tcaccacctc gctgcgcatt ggggcaagga ccgatggtgc gatggatatt accgtcgggc
    12061 cgctggtgaa tctgtggggc tttggcccgg aacaacagcc ggttcaaatt ccgagccagg
    12121 aacagatcga tgcgatgaaa gccaaaaccg gcttacagca cctgacggtc atcaatcagt
    12181 cgcatcagca atatctgcaa aaagacctgc cggatttata tgtcgatctc tccaccgtcg
    12241 gtgaaggtta tgcggcggat cacctggcac gcttgatgga gcaggaaggg atttcccgct
    12301 atctggtgtc ggtgggcggc gcgctgaaca gccgtggtat gaacggtgaa ggccagccgt
    12361 ggcgggtagc gattcaaaaa ccaaccgata aagaaaacgc ggttcaggct gtggtggata
    12421 tcaacggtca tgggattagc acctctggca gctaccgtaa ctattacgaa ctggacggca
    12481 aacgtctttc ccatgttatc gatccgcaaa ccgggcgtcc catcgaacac aatctggtat
    12541 ccgtgacggt gattgctccg acggcgctgg aagccgatgc ctgggatact ggcttgatgg
    12601 tactcgggcc ggagaaagcc aaagaggttg ttcgccggga agggctggcg gtttatatga
    12661 tcaccaaaga aggcgatagc tttaaaacct ggatgtcacc acagtttaaa agcttccttg
    12721 tcagcgaaaa aaattaaagc gcaagattgt tggtttttgc gtgatggtga ccgggcagac
    12781 taaaggctat ccttaaccag ggagctgatt atgaaaaacg ccacatgctt aactgacgat
    12841 caacgctggc aatctgtctt agcccgcgac ccgaatgccg acggcgaatt cgttttcgcc
    12901 gtgcgcacca cgggtatctt ttgccgtcct tcttgccgcg ccagacatgc cttgcgggaa
    12961 aatgtctcct tctacgcaaa tgccagcgag gcactcgccg ccggatttcg cccctgcaaa
    13021 cgttgtcagc cagacaaagc caatccccgg caacatcggt tggataaaat cacccacgcg
    13081 tgtcgactgc tggaacagga aacgcctgta acgctggaag ccttagccga ccaggtggcg
    13141 atgagtccat ttcatctaca tcggttgttt aaagctacta ccggaatgac gcctaaagcc
    13201 tggcaacagg cctggcgcgc tcgccgtttg cgcgaatcgc tggcgaaagg ggagagcgtg
    13261 acgacgtcta ttcttaacgc cggattcccc gacagcagca gttactaccg caaagccgat
    13321 gaaacgctgg gcatgacggc taaacaattc cgtcacggtg gcgaaaatct ggcggtgcgt
    13381 tacgcgctgg ctgattgtga gctgggtcgt tgcctggtgg cagaaagcga gcgggggatt
    13441 tgcgcgatat tgctgggcga tgatgacgcg acattaatca gcgagttgca gcagatgttt
    13501 cccgctgccg acaacgcgcc tgccgatctg acgtttcagc aacatgtgcg tgaagtgatc
    13561 gccagcctca atcaacgcga tacgccgctg acgttaccgc tggatattcg cggcactgct
    13621 tttcagcaac aagtctggca ggcactgcgc acgatacctt gtggtgaaac cgtcagttat
    13681 cagcaactgg ctaacgccat cggcaaaccg aaagcggtac gggccgttgc cagcgcctgt
    13741 gccgccaaca agctggctat cgtaataccc tgtcatcggg tggtccgtgg tgatggcaca
    13801 ctttccggtt accgctgggg cgtgtcgcgt aaagcgcaac tgctgcgccg cgaagctgaa
    13861 aatgaggaga ggtaatgttg gatctgtttg ccgatgctga accgtggcaa gagccactgg
    13921 cggctggtgc ggtaatttta cggcgttttg cttttaatgc tgcggagcaa ctaatccgcg
    13981 atattaatga cgttgccagc cagtcgccgt ttcgccagat ggtcgctcca ggcggatata
    14041 ccatgtcggt ggcgatgacc aactgtgggc atctgggctg gacgacccat cggcaaggtt
    14101 atctctattc gcccattgat ccgcaaacaa ataaaccgtg gcccgctatg ccacaaagct
    14161 ttcatgattt atgtcaacgt gcggctacgg cggcgggcta tccagatttc cagccagatg
    14221 cttgtcttat caaccgctac gctcctggcg cgaaactgtc gctgcatcag gataaagacg
    14281 aaccggatct gcgcgcgcca attgtttctg tttctctggg cttacccgcg atttttcaat
    14341 ttggtggcct gaaacgtaat gatccgctca aacgtttgtt gttggaacat ggcgatgtgg
    14401 tggtatgggg cggtgaatcg cggctgtttt atcacggtat tcaaccgttg aaagcggggt
    14461 ttcatccact caccaccgac tgccgctaca acctgacatt ccgtcaggca ggtaaaaaag
    14521 aataaaaata agaattatta ttgctgtgcg cgcgaagatg tttaaactgc gggctgtaat
    14581 tcattgtccg ggttttctgc atggaacttc ttgtacttgt ctggcggcag tatcgctggc
    14641 catttatcag tgtgatggcg ctaagcctcg ccagtgcggc attaggcatt ggcttaattg
    14701 cttttatcaa tcagcgcctt atcgaaacgg cggataccag tctgctggtg ttaccggagt
    14761 ttctgggatt attgctgctg ttgatggcag tcactctcgg atcgcaactg gcgctcacca
    14821 ctttggggca tcacttcgtt taccgactgc gtagtgaatt tatcaagcgg attctggata
    14881 cccacgtcga gcgcattgaa caactcggta gcgcctcgtt gctggcggga ttaaccagcg
    14941 atgtgcgcaa tatcaccatt gcttttgtgc gtctgccgga actggggcag gggatcattc
    15001 tcactatcgg ttcatcggcg tatctgtgga tgctgtcggg caaaatgttg ctggtaacgg
    15061 ctatctggat ggcgatcacc atctggggcg gttttgtgct ggtggcgcgg gtgtacaaac
    15121 atatggcgac cctacgtgaa accgaagaca agctgtacac ggattttcaa actgtacttg
    15181 aagggcgcaa agagctgact ctgaaccggg aacgcgccga gtatgtgttt aacaacctct
    15241 acatccccga tgcgcaagag tatcgccatc atattattcg cgcagacacc ttccatctta
    15301 gtgccgtgaa ctggtcaaac atcatgatgc tgggcgctat cggcctggtg ttctggatgg
    15361 cgaacagtct cggttgggct gataccaacg ttgctgcgac ctattcgttg acgctgttat
    15421 tcctgcgtac gccgctgctt tcggcggttg gcgcattgcc gacgctgctg acggcgcagg
    15481 tggcgtttaa caagctgaac aaattcgcgc tcgcgccttt caaagctgag tttccgcgcc
    15541 cgcaggcgtt tcccaactgg caaacgctgg agctgcgtaa cgtgacgttt gcttatcagg
    15601 ataacgcgtt ttccgttggt ccgattaatc tcaccatcaa acgtggcgag ctgctgtttc
    15661 tgattggcgg taacggtagc ggaaaatcga cgctggcgat gttgttgacg ggcttgtatc
    15721 agccacaaag cggcgaaatc ttgctggatg gcaaacctgt cagcggcgaa caaccggaag
    15781 attatcgcaa actgttttcg gcagtgttta ccgatgtctg gctgtttgat caactgctgg
    15841 ggccggaggg gcaacccgct aacccgcaac tggttgagaa gtggctggcg cagctgaaaa
    15901 tggctcataa gcttgagtta agcaacgggc gtattgttaa cctgaagtta tcaaaagggc
    15961 agaaaaaacg cgtggcgctg ttgctggcgc tggcagaaga acgcgatatt atcctgctgg
    16021 atgaatgggc ggtggatcag gatccacact tccgtcgtga gttttatcag gtgttgctgc
    16081 cgctgatgca ggagatgggt aaaactattt tcgctatcag tcatgatgat cattacttta
    16141 tccacgccga ccgcctgctg gaaatgcgca atgggcaact tagcgagctg acgggcgaag
    16201 agcgcgatgc cgcttcgcgt gatgccgttg cccggacggc ataatattac gacgctgacc
    16261 tggcggactg ctgccgtcag gtcaatatgc taaa
//
LOCUS       DAZSNM010000083        15885 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZSNM010000083 DAZSNM010000000
VERSION     DAZSNM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15885
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.083"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(6..1460)
                     /gene="xylB"
                     /locus_tag="ACHW42_002200"
     CDS             complement(6..1460)
                     /gene="xylB"
                     /locus_tag="ACHW42_002200"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR8944015.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELVRAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1532..2854)
                     /gene="xylA"
                     /locus_tag="ACHW42_002201"
                     /pseudo
     CDS             complement(1532..2854)
                     /gene="xylA"
                     /locus_tag="ACHW42_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            3220..4212
                     /gene="xylF"
                     /locus_tag="ACHW42_002202"
     CDS             3220..4212
                     /gene="xylF"
                     /locus_tag="ACHW42_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8944016.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            4290..5831
                     /gene="xylG"
                     /locus_tag="ACHW42_002203"
     CDS             4290..5831
                     /gene="xylG"
                     /locus_tag="ACHW42_002203"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFR8944017.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5809..6990
                     /gene="xylH"
                     /locus_tag="ACHW42_002204"
     CDS             5809..6990
                     /gene="xylH"
                     /locus_tag="ACHW42_002204"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFR8944018.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7068..8246
                     /gene="xylR"
                     /locus_tag="ACHW42_002205"
                     /pseudo
     CDS             7068..8246
                     /gene="xylR"
                     /locus_tag="ACHW42_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
     gene            complement(8354..9178)
                     /locus_tag="ACHW42_002206"
     CDS             complement(8354..9178)
                     /locus_tag="ACHW42_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFR8944019.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9498..11528
                     /gene="malS"
                     /locus_tag="ACHW42_002207"
     CDS             9498..11528
                     /gene="malS"
                     /locus_tag="ACHW42_002207"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR8944020.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11706..12959
                     /gene="avtA"
                     /locus_tag="ACHW42_002208"
     CDS             11706..12959
                     /gene="avtA"
                     /locus_tag="ACHW42_002208"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFR8944021.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13110..13583)
                     /locus_tag="ACHW42_002209"
     CDS             complement(13110..13583)
                     /locus_tag="ACHW42_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR8944022.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(13685..14533)
                     /gene="yiaJ"
                     /locus_tag="ACHW42_002210"
     CDS             complement(13685..14533)
                     /gene="yiaJ"
                     /locus_tag="ACHW42_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFR8944023.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LVHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            14733..15731
                     /gene="yiaK"
                     /locus_tag="ACHW42_002211"
     CDS             14733..15731
                     /gene="yiaK"
                     /locus_tag="ACHW42_002211"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFR8944024.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15743..>15885
                     /locus_tag="ACHW42_002212"
     CDS             15743..>15885
                     /locus_tag="ACHW42_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFR8944025.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         4037 a         4048 c         3897 g         3903 t
ORIGIN      
        1 aacgtttacg ccattaatgg cagaagttgc tgatagaggc gacggaacgt ttcacgtcgt
       61 ggctgataag cggcataacg ctgcgcatct ggtagatgcg actgttctaa cggtagctgc
      121 ggcaacaact caatgagcga tttctctgga ttcgccgcaa tctgcgccag ccttgctgcg
      181 cccagtgctg gccccacatc cccccccgta cggtaatcga gctgctgacc gctgatatcc
      241 gccagcatct gacgccagta ctcactacgc gccccgccac caatcaacgt aacactttgc
      301 ggtttaatgc cgcaggcatg cacgacatcc atgccatctg ccagcgcata acccacgcct
      361 tccagcactg ctcgcaccag ttcattgggg ccatgttgat gagtcaaacc aaagaaaacc
      421 cccttcgcct ggggattatt gtgtggtgta cgctcgccgg aaagataagg cagaaaccaa
      481 actggctcgg cactttcatc agcctgttga gctgcagcga ttaaagctgg gacattgcac
      541 aggccggtta atttcgcggc ccaatccaga cacgatgctg cactcagcat tacagacatt
      601 aaatgccaac gctgcggtag cgcatggcaa aagctgtgta cagcgctttc tggcttgctt
      661 aagaaccctt cgctgacagc aaaatagacc cccgacgtcc ccagcgataa cattgcctga
      721 ttagcatcaa ccattcccac accaactgca ccagctgcat tgtcgccacc gcctgcgaca
      781 actggcaccg tcgccatacc ccacgctttc gcaacttcag gtaacaaagc accagtaatt
      841 tcgctgcctt cgtataatgc gggcatctgg tcacgagata agtcgcaagc ctgcagcatg
      901 acgtcactcc agtcacgctt tgcgacatcc agccacatgg tgccagctgc gtcagacata
      961 tcgctggcaa actcccccgt catacgcaga cgcaagtaat ctttcggtaa taatactttg
     1021 tcgatttgac ggaatatctc cggctcatgc cgctgaaccc atagcaattt aggcgcagta
     1081 aatccgggca tcatcaggtt gccggtaatc actcgtgatt gcggaactct cgcttccagc
     1141 aaagtgcact cttgcgcaca gcgcccgtcg ttccacaaaa tggcagggcg caatacccgt
     1201 tgttgagcat ccagtaaggt tgctccatgc atctggccgg caatacccaa tgctttaacg
     1261 tcctgcagag aatgctgatc gcccagagct ttcattgcgc gatcagttgc ctgccaccac
     1321 tgttccgggt cttgttccga ccaaagtgga tgcgggcgcg aaacggtcag cttttccgtt
     1381 tgcgaagcaa ccacctcacc ctgctcgttg agcaaaataa cttttacgcc cgaggtgcca
     1441 agatctatcc cgatatacat atcgatcgtt ccttaaaaaa atgcccggta tcgctaccga
     1501 taaccgggcc aacggactgc acagttagcc gttatttgtc gaacagataa tgatttacca
     1561 gattttccag ttgctcctgg cgaccactct gatgcaccgg agacaaatta tgttcctgag
     1621 catatttggc taaatctgcc agtgacattt ggcctttcag gatttgctgg cccaattcgc
     1681 tattccagcc ggaataacgc tgcgcgatgc gtttatccag ctcgccatct tcaatcatgc
     1741 gcgctgcaat tttcagcgcc agtgccatcg tatccatcgc gccgatatga ccgtaaaaca
     1801 gatcatattt atcagtactt tgacgacgta ctttggcatc gaagttcaga ccaccggtgg
     1861 tgaaaccgcc tgctttgaga atttcataca tcaccagcgc attctcttcc acactgttcg
     1921 ggaactggtc ggtgtcccag cccagttgcg catcgccacg gttggcgtcg acagaaccga
     1981 acaggccaag cgcaatggcg gtggctattt catgatggaa agagtgacct gccagcgtcg
     2041 cgtggttagc ttcaatgttc agtttaatct ctttttccag accaaactgt ttcaggaagc
     2101 catagaccgt cgcggcatcg taatcatatt gatgtttagt cggttcttgc ggtttcggtt
     2161 cgataagcaa cgtgccctag aagccgattt tatgtttatg ctcaaccacc atctgcataa
     2221 agcggcccag ttgttcacgc tcctgacgca agtcggtatt taacagcgtt tcgtaacctt
     2281 cacgaccacc ccacaggaca tagttttcac cgcccaattt atgggttgct tccatcgctg
     2341 taacaacttg cgttgccgcc cagctgaaga cttcaggatc tgggttcgtc gccgcacccg
     2401 cgccatagcg agggtttgta aagcagttag cggttcccca cagcagcttc acgccgctct
     2461 cttcttgctt ggctgccagg acatcaacca tttgcgcaaa attattgatg tactctttta
     2521 acgacgcgcc ctcaggggaa acatccacat cgtggaagca ataaaatggc acatgtaact
     2581 tgtggaaaaa ctcaaatgcg acatctgctt tacgcttcgc caacgccagt gcctcaccag
     2641 gctgctgcca cggacgatta aacgccccca caccaaacat gtccgccccg ttccagcaga
     2701 aggtgtgcca gtagcaggcg gcaaaacgca agtgctcttc catacgctta cccaacacca
     2761 gttcgtcggg attgtagtga cggaatgcta acgggtttga ggattttgag ccttcataac
     2821 gaacgcgatc gagctggtca aaataggctt gcatattgaa ctccataatc aggtaatgcc
     2881 gcgggtgatg gatgatgtcg taatattggg cactcccttt caattgctca attatgttat
     2941 ttcacactgc tattgagata attcacaagt gtgcgctcgc tcgcaaaata aaatgggatg
     3001 atgaaaatgg gtaattcctc gaagataaaa atgcaataag tacaattgcg caacaaaagt
     3061 aagatctcgg tcataaatca agaaataaac caaaaatcgt aatcgaaaga taaaaatctg
     3121 taattgtttt cccctgttta gttgctaaaa attggttacg tttatcgcgg tgattgttac
     3181 ttattaaagc tgtcctctaa ctacagaagg ccctacacca tgaaaataaa gaacattcta
     3241 ctcacccttt gcacctcact tctgcttacc aacgttgcgg cacacgccaa agaagtcaaa
     3301 ataggtatgg cgattgatga tctccgtctt gaacgctggc aaaaagatcg agatattttt
     3361 gtgaaaaagg cagaatctct cggtgcgaaa gtatttgtac agtctgcaaa tggcaatgaa
     3421 gaaacacaaa tgtcgcagat tgaaaacatg attaaccggg gtgtcgatgt tcttgtcatt
     3481 attccgtata acggtcaggt attaagtaac gttgtaaaaa aagccaaaca agaaggtatt
     3541 aaagtattag cttacgaccg tatgattaac gatgcggata tcgattttta tatttctttc
     3601 gataacgaaa aagtcggtga actgcaggca aaagccttgg tcgatattgt tccgcaaggt
     3661 aattacttcc tgatgggcgg ctcgccggta gataacaacg ccaagctgtt ccgcgccgga
     3721 caaatgaaag tgttaaaacc ttacgttgat tctggaaaaa ttaaagtcgt tggtgaccaa
     3781 tgggttgatg gctggttacc ggaaaacgca ttgaaaatta tggaaaacgc gctaaccgcc
     3841 aataataaca aaattgatgc tgtagttgcc tcaaacgatg ccaccgcagg tggggcaatt
     3901 caggcattaa gcgcgcaagg tttatcaggg aaagtagcaa tttccggcca ggatgcggat
     3961 ctcgcaggta ttaaacgtat tgctgccggt acgcaaacta tgacggtgta taaacctatt
     4021 acattgctgg caaatactgc cgcagaaatt gccgttgaat tgggcaatgg tcaggagcca
     4081 aaagcagata cctcactgaa taatggcctg aaagatgtcc cctcccgcct cctgacaccg
     4141 atcgatgtga ataaaaacaa catcaaagat acggtaatta aagacggatt ccacaaagag
     4201 agcgagctgt aagcgttacg ccccggcgcg gagcgggggc gtgatttctc tccatgccgc
     4261 gtgaatgaat tggcttaggt ggagtcgtta tgccttatct acttgaaatg aagaacatta
     4321 ccaaaacctt cggcagtgtg aaggcgattg ataacgtcag cttgcggttg aatgctggcg
     4381 aaatcgtctc actctgtggg gaaaatgggt ctggtaaatc aacgctgatg aaagtgctgt
     4441 gtggtattta tccccatggc tcctacgaag gcgaaattat ttttgcggga gaagagattc
     4501 aggcgagtca catccgcgat accgaacgca aaggtatcgc catcattcac caggaattgg
     4561 ccctggtgaa agaattgacc gtgctggaaa atatcttcct gggtaacgaa ataacccaca
     4621 atggcattat ggattatgac ctgatgacgc tacgctatca gaagctgctc gcacaggtca
     4681 gtttatccat ttcacctgat acccgcgttg gcgatttagg gcttgggcaa caacaactgg
     4741 ttgaaattgc caaggcactt aataaacagg tgcgcttgtt aattctcgat gaaccgacag
     4801 cctcattaac tgagcaggaa acgtcggttt tactggatat tattcgcgat ctacaacagc
     4861 acggtatcgc ctgtatttat atttcgcaca aactcaacga agtcaaagcg atttccgata
     4921 cgatttgcgt tattcgcgac ggacagcaca ttggtacgcg tgatgctgcc ggaatgagtg
     4981 aagacgatat tatcaccatg atggtcgagc gagagttaac cgcgatttac cctaatgaac
     5041 cacataccac cggagatgaa atattacgta ttgaacatct gacggcatgg catccggtca
     5101 atcgtcatat taaacgagtt aatgatgtct cgttttccct gaaacgtggc gaaatactgg
     5161 gtattgccgg actcgttggt gccggacgta ccgagaccat tcagtgcctg tttggcgtgt
     5221 ggcccggaca atgggaagga aaaatttata ttgatggcaa acaggtagat attcgtaact
     5281 gtcagcaagc catcacccag gggattgcga tggtacccga agacagaaag cgcgacggca
     5341 tcgttccggt aatggcggtt ggtaaaaata ttaccctcgc cgcactcaat aaatttaccg
     5401 gtggcattag ccagcttgat gacgcggcag agcaaaaatg tattctggaa tcaattcagc
     5461 aactcaaagt taaaacgtcg tcccccgacc ttgctattgg acgtttgagc ggcggcaatc
     5521 agcaaaaagc gatcctcgcc cgctgtctgt tacttaaccc gcgcatcctc attcttgatg
     5581 aacctaccag gggtattgat attggcgcga aatatgagat ctacaaatta attaaccaac
     5641 tcgtccagca gggtattgcc gttattgtca tctcttccga attacctgaa gtgctcggcc
     5701 ttagcgatcg tgtactggtg atgcatgaag ggaaactaaa agccaacctg ataaatcata
     5761 acctgactca ggagcaggtg atggaagccg cattgaggag cgaacatcat gtcgaaaagc
     5821 aatccgtctg aagtgaaatt ggccgtaccg acatccggtg gcttttctgg gctgaaatca
     5881 ctgaatttgc aggtcttcgt gatgattgca gctatcatcg caatcatgct gttctttacc
     5941 tggaccaccg atggtgccta cttaagcgcc cgtaacgtct ccaacctgtt acgccagacc
     6001 gcgattaccg gcatcctcgc ggtaggaatg gtgttcgtca taatttctgc tgaaatcgac
     6061 ctttccgtcg gctcaatgat ggggctatta ggtggcgtcg cggcgatttg tgacgtctgg
     6121 ttaggctggc ctttgccact taccatcatt gtgacgttgg ttctgggact gcttctcggt
     6181 gcctggaacg gatggtgggt cgcgtaccgc aaagtccctt catttattgt caccctcgcg
     6241 ggcatgttgg catttcgcgg catactcatt ggcatcacca acggcacgac tgtatccccc
     6301 accagcgccg cgatgtcaca aattgggcaa agctatctcc ccgccagtac cggcttcatc
     6361 attggcgcgc ttggcttaat ggcttttgtt ggttggcaat ggcgcggaag aatgcgccgt
     6421 caggctttgg gtttacagtc tccggcctct accgcagtag tcggtcgcca agctttaacc
     6481 gctatcatcg tattaggcgc aatctggctg ttgaatgatt accgtggcgt tcccactcct
     6541 gttctgctgc tgacgttgct gttactcggc ggaatgttta tggcaacgcg gacggcattt
     6601 ggacgacgca tttatgccat cggcggcaat ctggaagcag cacgtctctc cgggattaac
     6661 gttgaacgca ccaaacttgc cgtgttcgcg attaacggat taatggtagc catcgccgga
     6721 ttaatcctta gttctcgact tggcgctggt tcaccttctg cgggaaatat cgccgaactg
     6781 gacgcaattg cagcatgcgt gattggcggc accagcctgg ctggcggtgt gggaagcgtt
     6841 gccggagcag taatgggggc atttatcatg gcttcactgg ataacggcat gagtatgatg
     6901 gatgtaccga ccttctggca gtatatcgtt aaaggtgcga ttctgttgct ggcagtatgg
     6961 atggactccg caaccaaacg ccgttcttga ttttgataaa aattttctca aagccggtta
     7021 cgtattaccg gttttgagtt tttgcatgat tcagcaggaa aagaaccatg tttactaaac
     7081 gtcaccgcat cacattactg ttcaatgcca ataaagccta tgaccggcag gtagtagaag
     7141 gcgtagggga atatttacag gcatcacaat cagaatggga tattttcatt gaagaagatt
     7201 tccgcgcccg cattgataaa atcaaggact ggttaggaga tgtcgtcatt gccgacttcg
     7261 acgacaaaca gatcgagcaa gcgctggctg atgtcgacgt ccccattgtt ggggttggcg
     7321 gctcgtatca ccttgcagaa agttacccac ccgttcatta cattgccacc gataactatg
     7381 cgctggttga aagcgcattt ttgcatttaa aagagaaagg cgttaaccgc tttgcttttt
     7441 atggtcttcc ggaatcaagc ggcaaacgtt gggccactga acgcgaatat gcatttcgtc
     7501 agcttgtcgc cgaagaaaag tatcgcggag tggtttatca ggggttagaa accgcgccag
     7561 agaactggca acacgcgcaa aatcggctgg cagactgact acaaacgctg ccaccgcaaa
     7621 ccgggattat tgccgttact gacgcccgag cgcggcatat tctgcaagta tgtgaacatc
     7681 tacatattcc cgtaccggaa aaattatgcg tgattggcat cgataacgaa gaactgaccc
     7741 gctatctgtc gcgtgtcgcc ctttcttcgg tcgctcaggg cgcgcggcaa atgggctatc
     7801 aggcggcaaa actgttgcat cgattattag ataaagaaga aatgccgcta cagcgaattt
     7861 tggtcccacc agttcgcgtc attgaacggc gctcaacaga ttatcgctcg ctgaccgatc
     7921 ccgccgttat tcaggccatg cattacattc gtaatcacgc ctgtaaaggg attaaagtgg
     7981 atcaggtact ggatgcggtc gggatctcgc gctccaatct tgagaagcgt tttaaagaag
     8041 aggtgggtga aaccatccat gcaatgattc atgccgagaa gctggagaaa gcgcgcagtc
     8101 tgctgatttc aaccaccttg tcgatcaatg agatatcgca aatgtgcggt tatccatcgc
     8161 tgcaatattt ctactctgtt tttaaaaaag catatgacac aacgccaaaa gagtatcgcg
     8221 atgtaaatag cgaggtcatg ttgtagcgat gatgagaatt gtcggcgtca catcagggaa
     8281 ctcagcacag ccacctgatg cgagcaaaag ccttatccga ccgacaaatc agataaggct
     8341 cgaggaaatg aaattacata tgcgccgcga ttaaccgttg gttatcctgg tacattgcga
     8401 acaggtagtt gttataactc ttccctttgg tcgagtagcc cttcagcttg tgaatcatcg
     8461 ctgtggcagt cacttcctga tccgctttac gcagctgtgc acgcgattta cggaacgaag
     8521 agtaagccgg gtgcgtattc aggttagtga cataggcgct caccgattct ttgacagaac
     8581 taaactgtga gtaccctttc actttacccg gcgcattggt acaacgtcct ttcatgcatt
     8641 tcatgccgaa cagattgtta ttgttgcgcg ccagcttcga cgttccccaa ccgctttctg
     8701 ctgcagccat cgtcgccacc atactggtag ggataatgtc tacgcgttca agcaaggtat
     8761 tccacgggat ttttcgcgta ttaccagacc acttcacctt gtagcgtttg gcgatgtctt
     8821 tcagacgcgc acgctcagca ggtgcccatt ggccctgata ctgttttgaa atcagccagt
     8881 tacgttctgc agtaatggct gcattttggc tggtaatgta aggcattacg gtccggagaa
     8941 acgccttttt ccttggtgtt ccggaagggt attttcgcaa atcaggaagt gaactactct
     9001 ttgcactatt gcgagaatac tcttgtttac tgcttacctg tttattacta gctttagtta
     9061 agtgggactt ttgactcgct gttgttgtgt gcgtcttcgc taacacctca ctcgaaaaca
     9121 ccagagtgag taacataaga atcatcgccc catatcgtcg tatgggagtc aaaatcatca
     9181 ggtctcctgg tcggatttaa tcattccaac accttatatt tttcacaaat ttgagagttg
     9241 aatctcaaat catatcaaaa atagctgtca agagcacccc aaggaatagt ccaaatctga
     9301 aactatgtca cgagttaacg attcagattg gcgctaaatc gcagaaaatg tgggggttat
     9361 cgcaaaattc agccgttttt tgcgcgcgat cgctcaccct tgcttctcat cctgtggact
     9421 taccgctcag ggatgagttt tgtctggctt atcgctggca aactgtctga aatcgcagca
     9481 ataaggactc atccaccatg aaactcgccg cctgttttct gacactcctt cctggcttcg
     9541 ccgttgccgc cagctggact tctccggggt tccctgcctt tagcgaacag ggaacgggaa
     9601 catttgtcag ccacgcgcag ttgcccaaag gtacgcgtcc actcacgcta aattttgacc
     9661 aacagtgctg gcagcctgca gatgcgataa aactcaatca gatgctttcc ctgcaacctt
     9721 gtagcaacac gccgcctcaa tggcgattgt tcagggacgg caaatatacg ctgcaaatag
     9781 acacccgctc cggtacgcca acattgatga tttccatcca gaacgccgcc gaaccggtag
     9841 caaacctggt ccgtgaatgc ccgaaatggg atggattacc gctcacgctg gatgtcagcg
     9901 ccactttccc ggaaggagcc gccgtacggg attattacag ccaacaaatt gcgatagtga
     9961 agaacggtca aataacgtta caacccgctg ctaccagcaa cggtttactc ctgctggaac
    10021 gggcagaaac tgacacatcc gcccctttcg actggcataa cgccacggtt tactttgtgc
    10081 tgacagatcg tttcgaaaac ggcgatccca gtaatgacca gagttacgga cgtcataaag
    10141 acggtatggc ggaaattggc acttttcacg gcggcgattt acgcggcctg accaacaaac
    10201 tggattacct ccagcagttg ggcgttaatg ctttatggat aagcgcccca tttgagcaaa
    10261 ttcacggctg ggtcggcggt ggtacaaaag gcgatttccc gcattatgcc taccacggtt
    10321 attacacaca ggactggacg aatcttgatg ccaatatggg caacgaagcc gatctacgga
    10381 cgctggttga tagcgcacat cagcgcggta ttcgtattct ctttgatgtc gtgatgaacc
    10441 acaccagcta tgccacgctg gcggatatgc aggagtatca gtttggcgcg ttatatcttt
    10501 ctggtgacga agtgaaaaaa acgctgggtg aacgctggag cgactggaaa cctgccgccg
    10561 gacaaacctg gcatagcttt aacgattaca ttaatttcag cgacaaaaca ggctgggata
    10621 aatggtgggg aaaaaactgg atcagaacgg atatcggcga ttacgacaat cctggattcg
    10681 atgatctcac tatgtcgctg gcctttttgc cggatatcaa aaccgaatca actaccgctt
    10741 ctggtctgcc ggtgttctat aaaaacaaaa cggatactca cgctaaagtc atcgaaggct
    10801 ttacacctcg cgattactta acccactggt taagtcagtg ggtccgcgac tatgggattg
    10861 atggttttcg ggtcgatacc gccaaacatg ttgagttgcc cgcctggcag caactgaaaa
    10921 ccgaagccag ctccgcgctt cgcgaatgga aaaaaactaa ccccgacaaa gcattagatg
    10981 acaaaccttt ctggatgacc ggtgaagcct ggggccacgg cgtgatgcaa agtgactact
    11041 atcgccacgg cttcgatgcg atgatcaatt tcgattatca ggagcaggcg gcgaaagcag
    11101 tcgattgtct ggcgcagatg gatacgacct ggcagcaaat ggcggagaaa ttgcagggtt
    11161 tcaacgtgtt gagctacctc tcgtcgcatg atacccgtct gttccgtgaa gggggcgaca
    11221 aagcagcaga gttattacta ttagcgccag gcgcggtaca aatcttttat ggcgatgaat
    11281 cctcgcgtcc gttcggtcct acaggttctg atccgctgca aggtacacgt tcggatatga
    11341 actggcagga tgttagcggt aaatctgccg ccaacgtcgc gcactggcag aaaatcagcc
    11401 agttccgcgc ccgccatccc gcaattggcg cgggcaaaca aacgacactt tcgctgaagc
    11461 agggctacgg ctttgttcgt gagcatggcg acgataaagt gctggtcatc tgggctgggc
    11521 aacagtgact tttccggctt cccgttcgtc agtacctcgg gaagccgcca accaggataa
    11581 aatgtcagcc ctaatcagcg ttgcgggata aagcaccgct cactcttcaa cagaccgatt
    11641 tgcaccccag caaatgtagc gttattgtta ccttccttgc tacagagttc gacagatatc
    11701 ccgctatgac attctccctt tttggtgaca aatttacccg ccactccggc attacgctgc
    11761 tgatggaaga tctgaacgac ggtttacgca cgcctggcgc gattatgctc ggcggcggta
    11821 atccggcgca gatcccggaa atgcaggact acttccagac gctactgacc gacatgctgg
    11881 aaagtggcaa agcgactgat gcactgtgta actacgacgg tccacagggg aaaacggagc
    11941 tactcacact gcttgccgga atgctgcgcg agaagttggg ttgggatatc gaaccacaga
    12001 atattgcact aacaaacggc agccagagcg cgtttttcta cttatttaac ctgtttgccg
    12061 gacgccgtgc cgatggtcgg gtcaaaaaag tgctgttccc gctggcaccg gaatacattg
    12121 gctacgctga cgccggactg gaagaagatc tgtttgtctc tgcgcgtccg aatattgaac
    12181 tgctgccgga aggccagttt aaataccacg tcgattttga gcatctgcat attggcgaag
    12241 aaaccgggat gatttgcgtc tcccggccga cgaatccaac aggtaatgtg attactgacg
    12301 aagagttgct gaagcttgac gcgctggcga atcaacacgg cattccgctg gtgattgata
    12361 acgcttatgg cgtcccgttc ccgggtatca tcttcagtga agggcgcccg ctatggaatc
    12421 cgaatatcgt actgtgcatg agtctttcca agctggggct gcctggctcc cgctgcggca
    12481 ttatcatcgc caatgaaaaa atcatcactg ccatcaccaa tatgaacggc attatcagcc
    12541 tggcacctgg cggtattggt ccggcgatga tgtgtgaaat gattaagcgt aacgatctgc
    12601 tgcgcctgtc tgaaacagtc atcaaaccgt tttactacca gcgtgttcag gaaactatcg
    12661 ccatcattcg ccgctattta ccggaaaatc gctgcctgat tcataaaccg gaaggagcca
    12721 ttttcctctg gctatggttt aaggatttgc ccattacgac cgagcagctc tatcagcgcc
    12781 tgaaagcacg cggcgtgctg atggtgccgg ggcacaactt cttcccggga ctggataaac
    12841 cgtggccgca tacgcatcaa tgtatgcgca tgaactacgt accagagccg gagaaaattg
    12901 aggcgggggt gaagattctg gcggaagaga tagaaagagc ctgggctgaa agtcactaaa
    12961 aattgcctga tgcgctacgc ttatcaggcc tacatgattt ctgcaatata ttgaatttgg
    13021 aagaatttgt aggccggata aggcgtttac gccgcatctg gcataaacaa agcgcactat
    13081 tccggcattg agaaacgccg gaaaacgttt caaacaggct gctgctgtag ccgatgccgc
    13141 tgcactttct cgacatccat gcactgcaac gcctttgtcg ggcaggcttc aacacaggcc
    13201 ggccccgtct cccgatgcca gcacagatcg cacttaatcg ccctcgcctt tttgcgcgaa
    13261 gaaacgacct ccatcgcacc aaacgggcaa gccagcatac agcttttaca gccaatgcaa
    13321 cgtgtttgtt caacgaaaat atgcccatgt tcgcggctta tcgcgtcaac agggcagaca
    13381 ttcgcgcacg gtgcatcttc acactgatga caggctactg gcgtggtcca gctgtggtct
    13441 ttaatgacac gaatacggga aataaactcg tctggtgaca acgcagcgca atcctgattc
    13501 tcctgatgcg acactgcgca agccacttca caggtacggc aaccgataca tttcgtcgca
    13561 tccgcaataa taaaccggtt catgatcatt cctttgcact aaaacagcag agtgccagag
    13621 agcgcgtccc tactgctttg atccaacgaa ggaaatgtta aagttttggc acttgtccaa
    13681 agcgttatgt gatcgcgccc aggtcatccc tgacagtaaa tcccagttca ttagaaatag
    13741 cctgcgcggt ttcacgcagt ggtttcagga gatttttctc tcccacctgt ttcagacgtg
    13801 atgtcgaaag cgaaatcgac acggcgtacg gcacccgccc atgaatatca aacaccggaa
    13861 cagcaataca ggagacgccg agttcgtttt cttctctgtc catcgccgct ccgctttcac
    13921 gaatgtgcgc cagttcgtcg aacatcgcgg gcagctcggt aatggtattg cgggttaacg
    13981 gctggatctc atgctgatgg ttttcccagt atgacttcac gtagtccggg tgaccaaacg
    14041 ccatgtagat cttgcccatt gcggaacagt agagcggcat atgctggcca atataggcac
    14101 gggttcgcag catcccggtt gtgggttcca gcttataaat caaaatagcg tgatcgtctt
    14161 cgcggctgga gaagttaatg gtttcaccag tggcgatgtt cagtgcctca agatgcggag
    14221 cggcgatatg aatgatattc agcgaagaca gcgccttctg cccgacggca ataaatttgg
    14281 tagtcaggcg gtaactccct gcggcgggcg cggtggtcac atagccgcag gactgtaatc
    14341 cctgcaataa gcgatggacg gtactcttat ttaaaccagc cagctccgaa agatgcacca
    14401 acggacaacc gtttggatag ttgctcaaaa tctcaatcag catcaacccg cgaaacagac
    14461 tctggcttcc ggctggacgc tctttttcct gcgccatctc gttctctttt tttcccatca
    14521 cttctttccc cattttgtcg cgtcctgatg gtagcgcaaa gtgtgccgta gttcacgatc
    14581 tcgacagata atttataacc acttgatttt tatgtctttt gaaattcatc aatcagattg
    14641 ctttgttaaa aagtgatcga tatatttgaa atctagttcc gcatattgaa attttaagcc
    14701 aaaaaagcga tcaaaaaaca aggaagccta ggatgaaagt gacatttgag cagttaaaag
    14761 cagcctttaa tcgggtctta atttcacgcg gcgttgacag cgaaacggct gacgcctgtg
    14821 cagagatgtt cgcccgcacc accgaatccg gcgtttattc tcacggcgtt aatcgtttcc
    14881 ctcgtttcat tcaacaactg gaaaacggcg atatcattcc tgatgcccaa cccaaacgta
    14941 taaccagcct cggcgcaatt gaacagtggg atgcccagcg ttcgatcggc aacctgacgg
    15001 cgaaaaagat gatggatcgc gccattgaac tggctgccga tcacggtatt ggtctggtgg
    15061 cacttcgtaa tgccaaccac tggatgcgcg gcggcagcta cggctggcag gcggcggaaa
    15121 aaggctatat tggcatttgc tggaccaact ccatcgccgt aatgccgccg tggggcgcaa
    15181 aagagtgtcg catcggcact aacccgctga tcgtcgccat tccttccacg ccgatcacca
    15241 tggtcgatat gtcgatgtcg atgttctctt acggcatgtt agaagttaac cgcctggcag
    15301 gccgtcagct cccggtcgat ggtggctttg atgatgaggg caatttgacc aaagaacctg
    15361 gcgttatcga gaagaatcgc cgcattttgc cgatgggcta ctggaaaggt tctggcatgt
    15421 cgattgtgct ggatatgatc gctactctcc tttccgacgg cgcatcggtt gccgaagtca
    15481 cccaggacaa cagcgacgaa tacggcattt cacaaatttt tattgccatt gaagtggaca
    15541 agcttatcga cggtcccacc cgcgatgcca agctgcaacg tatcatggat tacgttacta
    15601 ccgctgaacg cgctgacgaa aaccaggcca tccgcttacc cggccacgaa tttactaccc
    15661 tgctggccga aaaccgccgt aacggcatca ctgttgatga cagcgtgtgg gccaaaatcc
    15721 aggcgttatg aggagataag tcatgatttt tggacatatc gcgcagccca atccgtgccg
    15781 tttgcccgcc gccattgaaa aggcgctcga ttttctgcgc gctaccaact tcaacgtcct
    15841 ggagccgggc gttgtcgaaa tcgacggcaa gaatatttat gcgca
//
LOCUS       DAZSNM010000084        15847 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZSNM010000084 DAZSNM010000000
VERSION     DAZSNM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15847
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.084"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHW42_002213"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHW42_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
     gene            complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHW42_002214"
     CDS             complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHW42_002214"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR8944026.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHW42_002215"
     CDS             complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHW42_002215"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR8944027.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHW42_002216"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHW42_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="ACHW42_002217"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="ACHW42_002217"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR8944028.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="ACHW42_002218"
     CDS             complement(5877..6218)
                     /locus_tag="ACHW42_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR8944029.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHW42_002219"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHW42_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR8944030.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHW42_002220"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHW42_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR8944031.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12583
                     /gene="msrA"
                     /locus_tag="ACHW42_002221"
     CDS             11936..12583
                     /gene="msrA"
                     /locus_tag="ACHW42_002221"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR8944032.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGGIGVCLPPEA"
     gene            12906..14249
                     /locus_tag="ACHW42_002222"
     CDS             12906..14249
                     /locus_tag="ACHW42_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR8944033.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14311..14517)
                     /locus_tag="ACHW42_002223"
     CDS             complement(14311..14517)
                     /locus_tag="ACHW42_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR8944034.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14842..15396
                     /locus_tag="ACHW42_002224"
     CDS             14842..15396
                     /locus_tag="ACHW42_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR8944035.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15459..>15847)
                     /gene="ytfI"
                     /locus_tag="ACHW42_002225"
     CDS             complement(15459..>15847)
                     /gene="ytfI"
                     /locus_tag="ACHW42_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR8944036.1"
                     /translation="ELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDE
                     RFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEK
                     YKSTIEAFKKEQLALPVLVRCKNSET"
BASE COUNT         3758 a         4349 c         3913 g         3827 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc ggaattggcg tctgtctgcc gccggaagca tagcgttacg ggtacaaatg
    12601 tagtttgttg ataaagtgct ctttgtctat gcctgatgcg gcttaaacga cttaccaggc
    12661 ctacaaaatc gcccaaattc aatatattgc aaggactgcg taggcctgat cggcatagcg
    12721 catcaggcag tattgcattt atcagcagtc tgaattttaa ccctctggcg actttacagt
    12781 accttacgct atactagcca ctgaaaatgc cggttcactt tcttcgaatc ggctttcaat
    12841 gtgtatttca cacaaattaa tcaactttcc cttccgaggg tctggcctga aaggtcggat
    12901 aagatatgtt aaacagtatt ttagtcatac tctgcttgat cgctgtaagt gcgttcttct
    12961 cgatgtccga gatctcgctt gccgcctcac gcaaaatcaa acttaaactg ctggctgatg
    13021 aaggcaatat aaatgcccaa cgcgttctga atatgcagga aaatcccggc atgttcttta
    13081 ccgtggtcca aatcggtctg aacgcagtgg cgattctcgg cggtatcgtc ggtgatgcgg
    13141 cattttctcc agcttttcac agcctgttct cccgctatat gtcggcagag ctctctgagc
    13201 aactgagctt tattctctct ttctcgttag tgactggcat gtttatcctg tttgcggatt
    13261 taaccccgaa acgcatcggt atgattgcgc cagaagcggt ggctttgcgt atcatcaacc
    13321 cgatgcgctt ctgcctgtac gtttgcaccc cgctggtgtg gttcttcaac ggcctggcga
    13381 acatgatctt ccgtattttc aaactgccaa tggtacgtaa agatgacatc acttctgatg
    13441 acatctacgc ggtagtggaa gccggtgcgc tggcgggcgt gttacgtaaa caggaacacg
    13501 agctgattga aaacgtcttt gagctggaat cccgtaccgt tccgtcttca atgacaccgc
    13561 gtgaaaacgt gatttggttt gatctccacg aagatgagca aagcctgaag aataaggtgg
    13621 cggaacatcc gcactctaag ttcctcgtct gtaatgaaga tattgaccac atcatcggtt
    13681 atgtcgattc taaagacctg ctgaaccgcg tgctggctaa ccaaagcctg gcactgaaca
    13741 gcggcgtaca aattcgcaac acgctgattg tgccggatac gttaaccctt tcagaggcgt
    13801 tggaaagttt taaaaccgca ggtgaagact tcgcggtgat catgaacgag tacgcgctgg
    13861 tggtggggat catcaccctc aacgacgtga tgaccacgct gatgggcgat ctggtcggtc
    13921 aggggctgga agagcagatt gtcgcccgtg atgagaactc atggctgatt gacggcggca
    13981 ccccaattga cgacgtcatg cgcgtgctgg atattgacga gttcccgcag tcgggcaact
    14041 acgaaaccat cggcggcttt atgatgttta tgctgcgtaa gatcccgaaa cgcaccgatt
    14101 cggtgaaatt cgccggctac aaatttgaag tggtggatat cgataactac cgcatcgacc
    14161 agctgctggt gacccggatc gacagcaagg ccaccgccct ttcgccaaaa ctgcctgacg
    14221 ctaaagataa agaagaaagc gtcgcgtaac ccccagaaac atcaacggct cctgaatcag
    14281 gagccgtctt attactgcat agcactttgg ttaagccatc tctgtttgca gacgcataac
    14341 ctgacggtta acttcggaca tcactgacaa atgcagctta tccttcactt ttgggataag
    14401 aatcttaccc ttatcaaatt caaaagcgcc aatgtccttg atgtataacc gtccacggaa
    14461 caggatcttt acgtacttcg ccacctgaag tgggttgtac cgttggaaaa ttttcattgt
    14521 tatctcctgc tgagtattac gcctgtgcgg tgccacaatc ggcccaacta ttatgaggcg
    14581 caaattttaa tgcttagtga ctatagacta tccgggcaat gtttccaccg cgtataactt
    14641 tttttaccta aaggttacaa ttattcagaa ttatcttttt accgaagcgc ggtcttcagt
    14701 ataagcattc atttttcata tgatttgtgc gcttgaccgc aaactggcat cacacttgcg
    14761 ggaaattcga taaatagcac atatgattaa aactcagacc caagtggtcg gatcacctgc
    14821 atatcataag aaggaaacac catgacccta cgcaagattc tggcactcac ctgcctgctg
    14881 ttgccgatga tggcttccgc acatcagttc gaaatcggtc agcgagtgcc gccgattggc
    14941 attaccgatc ggggcgagtt ggtgcttgat aaagatcagt ttagctacaa aacctggaac
    15001 agcgcgcagt tagtgggaaa agtgcgagta ctgcaacata ttgctggtcg cacctctgca
    15061 aaagagaaaa acgcgacgct gattgaagcg attaaatcag cgaagttacc gcacgatcgt
    15121 taccagacca ccaccattgt taacaccgac gacgcgattc cgggttccgg catgtttgtg
    15181 cgcagcagtc tggagagtaa taaaaagctt tatccctggt cgcagtttat tgtcgatagc
    15241 aatggcgtcg cgcgcggtgc ctggcagctg gatgaagaaa gttccgctgt agtggtgctg
    15301 gataaagacg gtcgcgtgca atgggccaaa gacggggcgc tcacaccgga agaggtgcag
    15361 caagtgatgg acctgctgca aaagttgctc aaataatggc aataacataa attctggaaa
    15421 aataattttc ccctatcgtt atctatggga taggggaatt aagtctctga gttcttacac
    15481 cgaactaata ctggtaacgc tagttgctct tttttaaacg cctcaatggt cgatttatat
    15541 ttctcgcaaa tcttttcttt ggtaatatta ctatctgcgg ccagattcgc acttactatc
    15601 gcatccactt gttcggctaa ttcgacttgt tggcaacctg cattaaccag aaaattataa
    15661 ataagagagt aatcttcatc ctccaaaaca acagaggaac cattacgaaa acgttcatcc
    15721 cctccataga ttttaagcgc aaagcccaaa gattctatga ctatggcaaa attatcatca
    15781 attatttttt cgtaaatata ccatccatcg cattctttaa ttccaccgcc tggtatttgg
    15841 agttcaa
//
LOCUS       DAZSNM010000085        15759 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZSNM010000085 DAZSNM010000000
VERSION     DAZSNM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15759
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.085"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            94..216
                     /locus_tag="ACHW42_002226"
     CDS             94..216
                     /locus_tag="ACHW42_002226"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944037.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
     gene            213..779
                     /gene="mntP"
                     /locus_tag="ACHW42_002227"
     CDS             213..779
                     /gene="mntP"
                     /locus_tag="ACHW42_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR8944038.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(776..1585)
                     /gene="rlmA"
                     /locus_tag="ACHW42_002228"
     CDS             complement(776..1585)
                     /gene="rlmA"
                     /locus_tag="ACHW42_002228"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR8944039.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1751..1960)
                     /gene="cspE"
                     /locus_tag="ACHW42_002229"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1751..1960)
                     /gene="cspE"
                     /locus_tag="ACHW42_002229"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR8944040.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(1973..2116)
                     /locus_tag="ACHW42_002230"
     CDS             complement(1973..2116)
                     /locus_tag="ACHW42_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR8944041.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(2787..3074)
                     /locus_tag="ACHW42_002231"
     CDS             complement(2787..3074)
                     /locus_tag="ACHW42_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR8944042.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3149..3292)
                     /gene="mgrB"
                     /locus_tag="ACHW42_002232"
     CDS             complement(3149..3292)
                     /gene="mgrB"
                     /locus_tag="ACHW42_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR8944043.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3451..3690
                     /locus_tag="ACHW42_002233"
     CDS             3451..3690
                     /locus_tag="ACHW42_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR8944044.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(3833..4624)
                     /gene="kdgR"
                     /locus_tag="ACHW42_002234"
     CDS             complement(3833..4624)
                     /gene="kdgR"
                     /locus_tag="ACHW42_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR8944045.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            4801..6180
                     /locus_tag="ACHW42_002235"
     CDS             4801..6180
                     /locus_tag="ACHW42_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8944046.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAIRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSASAP
                     RERSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGL
                     RITQPRARA"
     gene            complement(6226..7107)
                     /gene="htpX"
                     /locus_tag="ACHW42_002236"
     CDS             complement(6226..7107)
                     /gene="htpX"
                     /locus_tag="ACHW42_002236"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR8944047.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7299..9347)
                     /gene="prc"
                     /locus_tag="ACHW42_002237"
     CDS             complement(7299..9347)
                     /gene="prc"
                     /locus_tag="ACHW42_002237"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR8944048.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9367..10065)
                     /gene="proQ"
                     /locus_tag="ACHW42_002238"
     CDS             complement(9367..10065)
                     /gene="proQ"
                     /locus_tag="ACHW42_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR8944049.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10162..10659)
                     /gene="msrC"
                     /locus_tag="ACHW42_002239"
     CDS             complement(10162..10659)
                     /gene="msrC"
                     /locus_tag="ACHW42_002239"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR8944050.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            10789..12072
                     /gene="yebS"
                     /locus_tag="ACHW42_002240"
     CDS             10789..12072
                     /gene="yebS"
                     /locus_tag="ACHW42_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR8944051.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12041..14661
                     /gene="yebT"
                     /locus_tag="ACHW42_002241"
                     /pseudo
     CDS             12041..14661
                     /gene="yebT"
                     /locus_tag="ACHW42_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14741..>15759
                     /gene="rsmF"
                     /locus_tag="ACHW42_002242"
     CDS             14741..>15759
                     /gene="rsmF"
                     /locus_tag="ACHW42_002242"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFR8944052.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTYTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQ"
BASE COUNT         3784 a         4015 c         3810 g         4150 t
ORIGIN      
        1 gccgatttcc agattccgga aatgtacgtg tcaacatact cgttgcaaaa cgtggcacgt
       61 acggactgaa gactttcagt caggcgagac catatgcaca tcaatcgcta tgcctgcatg
      121 aacgcgaaaa cccatcgcgg cttgttgttc atgtatgcat gtttgctggg ggcgatgatg
      181 tgttttatgg ataccccggt caggacgttg tcatgaatat cactgctact gttcttcttg
      241 cgtttggtat gtcgatggat gcatttgctg catcaatcgg taaaggtgcc cccctccata
      301 aaccgaaatt ttctgaagca ttgcgaaccg gccttatttt tggtgccgtc gaaaccctga
      361 cgccgctgat cggctgggga atgggcatgt tagccagccg gtttgtcctt gaatggaatc
      421 actggattgc gtttgtgctg ctgatattcc tcggcgggcg aatgattatt gagggttttc
      481 gtggcgcaga tgatgaagat gaagagccgc gccgtcggca cggtttctgg ctactggtaa
      541 ccaccgcgat tgccaccagc ctggatgcca tggctgtggg tgttggtctt gctttccttc
      601 aggtcaacat tatcgcgacc gcattggcca ttggttgtgc aaccttgatt atgtcgacat
      661 tagggatgat ggttggtcgc tttatcggct caattattgg gaaaaaagcg gaaattctcg
      721 gcgggctggt gctgatcggc atcggcgtcc agatcctctg gacgcacttc cacggttaat
      781 aagaacgctg ccagaggtga atattaaagt ccgtctggca gtcgaacact tcttttgctg
      841 ccagtgtttg ccagacttct ggcttcgcac gccaggcaaa cggcgtcatc tgcaataatg
      901 cgacggcttc atcaccgcga agacgcatcg gataacacaa ctccgcactc tgctgtaatg
      961 taaaaccttc cagttgttct gcatgaggtg catgaagatg tacttcattg taaatcagcc
     1021 ccttcagctc catcaaatgt cgcggtcccg gcgtggcagt aatgacccag ccgccgggct
     1081 tcactactcg tgctaattct tctgctttac acggcgcgta aatacgtatt atggcgtcca
     1141 tactggtatc ggaaaacggc aaacggtggc tggaagcgac acaaagagtg acctgcggat
     1201 agcgtttcgc cgccgctttt atcgctacct tcgagacatc caggccaaac gtggtgattt
     1261 cgggcaacgc atcggcaaat gcgtgtgtgt aatacccttc accacagcca atatccagca
     1321 ccgccgtggc cttttcatca agccgttccc gcagttgggc gacaattgca tcacgcagcg
     1381 gctgataatg tccggcatct aagaatgcgc ggcgtgcttg catcatttcc gcgctgtcgc
     1441 ccggatcacg agaccgttta tgctgaacgg gcagcagatt gacataccct tctttcgcca
     1501 tatcaaactg atgtcgttgg ggacagatat agctgttttt ttcacgcgaa agaggctgat
     1561 ggcaaagtgg acaggaaaac gacatgacaa ctccggcagg atactaaagg ccgcaagtgt
     1621 aacgcgaatc acgcggcggg caaatatccg aacgccagga gcgaagataa agcatagtaa
     1681 aaagcctcgc attcgacgag gctttatagc gagattgaag cgtattcaca cttcagatca
     1741 gtggattcga tcagatagct gttacgttaa cagctgccgg acctttctgg ccgtcctgaa
     1801 tttcgaactc aacgttctga ccttcagcca gagttttgaa gccattaccc tggatagcgg
     1861 agaagtgtac gaacacatct ttgctgccat cagccggagt aatgaagcca aaacctttag
     1921 actcgttgaa ccacttaacc tgacctttaa tctttgccat ttgaaaaatt ccttagattg
     1981 ttttcttcgc ccgcaggcat aacatagata aaactgacac attactgcat gaggcaccaa
     2041 tataaggctc ggcagagaag cggtattcaa cgtcaacgtg tttactcagg acttctttac
     2101 tgaaaatgcc acacataaac agaactgtac ctcgtttaac ccgaaatctt ttatcacata
     2161 caacgttaat tatggcaagc catttttaaa catatctcga tcagacgcac aaatctcgat
     2221 acgtcttcca ctttttttgc acacttatgc aacggaatac gcgccgatat atcattgtgc
     2281 ttaaccttgc cagttcaggc agatacttaa actggcgtag tttctaacat agttcaataa
     2341 gttcagttgt tccaggccag agggagaagt tattacatag tgcgtgcaat atcacatttt
     2401 ctgctatgca atgaataaaa agttatatca ctttttctca taaaacagtc agttaacggc
     2461 tatcaattac cctaaagaga agtcaatccc caaagggatt gtaaatttaa aataagaaaa
     2521 attgatgagt gagcaaaaaa aatcaagaga gaaacgtttc gctaataaat aataccacgc
     2581 ttaatcacaa atctgatgta aacatcctaa taaattaatg aggaaaatat ctttttctga
     2641 tatcccgtta cgcaaataaa taatatgata attatttgaa gtcagaccag agttttaatt
     2701 tgaagcagtt gcaccatgac agtgcgtata atcaggatga attgagtaac ccttcaacga
     2761 cggggttact caagattgag gaaggattat cgttcggcga ccaggcgaat aatatcttcg
     2821 tcatcaaccg atttttgact ctcaatggtc tttttatctg ctttctctgg ttcgtttgct
     2881 tcgcacaaac gacgtaacag tgcattttga cgtttttgct gatccagcaa cgcctcaagc
     2941 agttcaatct gttcgttagt ccgtgaactg gcacgattga taaaaaacca caagatgagc
     3001 ccgataagaa gaaccaccac cgatacaacc aaagacgcaa tgttcatcac gcctgaattt
     3061 acaacttcgt tcatttcacc acctctgagt agaggcgcta ttctaccact gctggagagg
     3121 aagaaaatct agtgctgaaa aaatgatatc accacgggat aaactggtta atggcacaaa
     3181 ttccgctgaa aaattgtaca tcctgatcgc acatcatgtt gaatacctgc gcccaaagca
     3241 gcaagcaagc caacaccacg acaaccagaa cgacccatcg aaactttttc actccaatct
     3301 ccgcttcacc taccttatgt cattaaacta gcatgcgtta gttaaacagc gcctaactat
     3361 gtcgatattc gtgctttttc ggaatgagta gcttgcttca gcgcatattg ccgctacgat
     3421 taagcgaaca taaaaagaga agagattgta atgcgattca tcattcgcac agttatgctg
     3481 atagcactgg tatggatagg attattactc agcggatatg gcgttttgat tggaagcaaa
     3541 gagaatgccg caggattagg tttgcaatgt acctatctga ctgccagagg caccagcacg
     3601 gtacaatact tgcacactaa aagtggtttc ttggagataa cagattgccc actgttacgt
     3661 aaaagcaata tcgtggtcga taatggttaa gggtaataaa taaccagatt ctcactcatg
     3721 agtaaaatat tcttatagca agcgcgttta atataacgca ccttcaactt tatttttctc
     3781 atgctaaata aagaacctgt ctgtttgata ttccgacagg ttcttactct catcagaacg
     3841 gatagtcgtg ataacccatt tgggcagaaa ttttgcgcgc tgcggtatgc aacattgcga
     3901 catattcctg taaacgctct tcagagaaac gcaacgtcgg gaaggagatg ctcaaaccgg
     3961 caatgaccac gccaaagcga tcaaataccg gtaccgcaat gcattgcagc ccttcttcct
     4021 gctcttcgtt atcttctcca tacccctgct cgcgcacctg gtccagaacg ggtaataacg
     4081 cttctgtact ggtgatggtc cgctcggtac tgcgtttata ctccacgccc tcaagaattt
     4141 gcttcacttc atcgcgatcg cgccatgcca gcagtacctt accaatcgcg gtgctgtaca
     4201 gcggattacg acgcccaatc cgtgaataca tgcgcaaatt gtacatagag tcaattttgt
     4261 gaatgtaaac aatactgtct tcgtccagtg cgccgaggtg gatagtttct ttggtcaggc
     4321 gggagatctc acgcatctgg atatctgcgc tacgaattaa atcgacgttt tgtaacgcgc
     4381 gagcgcccag ttcaaacaat ttcagggtca gggaatattt ctccgactcc ccttcctgcg
     4441 ccacataacc taaggttttc atggtctgta aaaagcgata aacggtgctt tttgacatca
     4501 tgacgcgctg cgacagctcg gttatcccta tttcgcgctc ttcacccagc gcctgcaaaa
     4561 tgccaaaaac ttttagcacg gaagatacag aatcaggctg tttatccaga tctgcgttag
     4621 ccatttatca cctcattgcg agtgttttat aaaaatcaga actgtttttt attataattt
     4681 cgcaccaggg tggtcgcaat ccatcttttg ccggttagtt acaattctgc gacatccacc
     4741 gtgaatatca gtgctagaat catacccctg ttgattattc accaaagata taaaattcct
     4801 atgccaaaag ttcaggccga cggcctgcca ttgccccagc gatacggtgc gatattaacc
     4861 attgtgattg gtatttcgat ggctgtcctt gacggcgcaa tcgccaacgt cgccctgcca
     4921 acaattgcca cggaccttca tgccacacca gccagttcca tctgggtagt gaacgcctat
     4981 caaatcgcca ttgtcatctc cctgctctcg ttttcgtttc tgggcgatat gtttggctat
     5041 cgacgtattt ataaatgcgg tctggtcgtt tttctgttgt cttcactgtt ctgcgccctt
     5101 tctgattcgc tgcaaatgct cacccttgcg cgtgtcatac aaggtttcgg cggtgcagcg
     5161 ttgatgagcg ttaataccgc acttatccgc ctgatctatc cacaacgttt tctggggaga
     5221 gggatgggca taaactcgtt tattgttgcc gtctcttctg ctgccgggcc gacaattgct
     5281 gcagcaatcc tctccatcgc atcctggaaa tggttatttt taatcaacgt accgttaggt
     5341 attatcgccc tgcttctggc gatacgtttt ctgccaccca atggttctcg cgccagtaaa
     5401 ccccgtttcg acctgcccag cgccgtgatg aacgcgttaa ccttcggcct gcttatcact
     5461 gcgttgagtg gtttcgctca ggggcaatcg ctgacgttaa ttgctgcgga actggtggta
     5521 atggttgttg ttggtatttt ctttattcgc cgccagcttt ctcttcccgt accgctgcta
     5581 ccggtggatt tactgcgtat cccgctgttt tcactttcta tttgcacatc tgtttgctct
     5641 ttctgcgcac aaatgctggc aatggtttcc ctgccctttt acctgcaaac cgtgctcggg
     5701 cgtagtgaag tcgaaacagg tttacttctg acaccgtggc cgttagcaac gatggtgatg
     5761 gctcctctgg caggctattt gattgaacgc gtacatgcag gattgctggg tgctttaggg
     5821 ttgttcatca tggctacggg gcttttttcc ctggttctgc tgcccgcgtc acctgcggat
     5881 atcaatatta tctggccgat gatcttatgt ggcgctggat ttggcttatt ccagtcaccc
     5941 aataaccaca ccattattac ctccgcctcc gctccgcgcg aacgtagcgg tggggccagt
     6001 ggcatgttag gaacggctcg tctactgggt cagagtagtg gcgcggcgct ggtggcgctg
     6061 atgctaaatc agtttggtga taatggtacg cacgtctcgc tgatggctgc ggctattctg
     6121 gcagtgattg ctgcctgtgt cagtggttta cgtatcactc agccacgagc cagggcataa
     6181 aaaaagcgcg tcgatcagga cgcgcttttt agtatttact tcatattact tcaggtattc
     6241 acccgtacgc agagcttcaa tacgtttatc cagcggcggg tgagtcatga acaactcact
     6301 gagcgatttc gacttaccgt taatgcagaa agccatcatg ctggttgctt cttgcggttc
     6361 atagctggtt ttcaggcgct gcagcgcggc aatcattttc tcgcgaccaa ccagttttgc
     6421 cgaaccggca tctgcatgga attcacgatg acgcgagaac cacatggtga taatgctcgc
     6481 cagaatacca aacaccagtt ccagaaccgt tgcaaccgca aagtagatca gcgggttgcc
     6541 gttgctctct tcaccttcat cacgatttcc gcccataaaa cccgcggcaa gctgcgccag
     6601 aatacgggaa ataaagataa cgaaggtgtt caccacgccc tgaatcagcg tcatggtgac
     6661 catatcacca ttggcgatgt ggctgatttc gtgagcaatt accgcctcgg cttcatccgg
     6721 gctcatgttc tgcagcaaac cggtgctgac agcaaccaga gaggcatcac ggcgcgcacc
     6781 ggttgcaaaa gcgttgatgt ccggcgcatg gtagatagcc acttgcggca tagcgatccc
     6841 cgcctgacga gcctgggttg ctacagtatt gaccagccaa cgttcccttt cgttacgcgg
     6901 ttgctcgatc acttccccgc caacagatcg taatgccatc catttggaca tcagaagcga
     6961 aacgaaggaa ccaccaaaac cgaacagcaa ggccatgatc atcagcccct gaacgctgct
     7021 cgactgtatc cctgtcaggc tcagtaccag cccgaaaacg accattacgg ccaggttcgt
     7081 tagcaggaag agcgcgattc gcatcataat tttcttttta cctcagttta acaaaacgca
     7141 atatgcgata cccacatcgt atgggttacg cgactatttt caagtctgga tagtgcgtaa
     7201 gtcaccagaa agacacaact ttacattttg tagcatctga tttacggcat cttgccgctg
     7261 ttaaaaaatc aggcacaatt tcttgtgcct gattgatatt acttgacggg agcgggttgt
     7321 tccgcgggtc tggctttttc aagcttcgcc agatcgagtg cgatattcac cgtctcatcc
     7381 agataaggat ccggctcctg gtaatctttc gatagatcat ccagcttctt caactccggt
     7441 ttaccttctc gtttaaagcg ttcgttcaaa cgcgccagac gcgtcgcatc atcttcatta
     7501 ttctctttct cacgcacagc gtagttcaga gaaacgatat tgcgcttgtc cttcatagcg
     7561 ttgaagcgcg cgatatcctt catgatgttc tggaactcag gatctttcgc gatacgcgca
     7621 ttatgttcct tcagcagctc cggttcaaag gcagttaaat ctcctgattt cacataagtc
     7681 gcggcatcga tgctatccca cggcagcgcg ttatcttcga atttctcacc cgtttccgtt
     7741 tcttcattac ccgtcggcat gatgatgtct ggtgttacgc ctttacgttg cgtactgccg
     7801 ccgttaacgc gatagaattt ctggatcgtg tactgcacag aacccagcgc tggccattca
     7861 ggacgtaaca tctgatcgta aatacggttc aatgaacggt attgctgaac ggtgccttta
     7921 ccaaacgtcg gttcacccac aaccagcgca cgaccgtaat cctgcattgc cgcggcaaag
     7981 atttctgaag ccgaagcact gaaacggtca accagtacca ccagcgggcc tttatagaac
     8041 acctgcccgt cggtatcgct gtcttcacga accttgccgt tgttatcgcg gacctgaaca
     8101 atgggacccg caggaataaa cagacctgag agcgataccg cttcggtcaa cgccccaccg
     8161 ccattgctac gcaggtcgat gatcacactg ctgacattct gtttttccag tttctgcagt
     8221 tgaactttga catcgtctgt caaacccaca tagaagcccg gaatatccag cacgccgact
     8281 ttctctttac cgacggtctt caccgacatt ttaaccgcgc ggtcttcgag acgaatacgt
     8341 tcacgggtca acgttacagt acgggtcttg gtccctttac cagcaggtaa aatttccaga
     8401 cgaactttac tgcccttcgg ccctttaatt aaggcaacca catcatcaag acgccagcca
     8461 atcacgtcaa ccatcggctt gcctgtttga ccaacaccga caattttgtc accaacgctg
     8521 atagctttac tcttcgctgc cggaccacct gccaccatcg aattgataac ggtgtagtca
     8581 tcatccattt gcagcactgc gccaatacct tccagtgaca aactcatttc agtgttgaac
     8641 tgttcggtat tacgcgggga aagatagttg gtatgcggat cgatttcacg cgcaaacgcc
     8701 gtcattgcca gcgagaaaac atcttcgctg ttggtttgcg ccagacgacg aatggcaaat
     8761 ttgtagcggc gtgtcagggt ttcacgaatt tctttatccg tttttcctgt cagcttcagg
     8821 cttaactcgt cgaatttgac tttactgtcc cactgcgcgt tcaactcagc ctcgtttttc
     8881 ggccagggcg ctttgctgcg gtcaaggtta taagtgtcgt tgccggtgaa atccatcggc
     8941 ttttccagta ccgacaaagc gtactggtaa cgttcaaaac ggcgcttttg cgccagattg
     9001 tagagatcgt agaaaacgtc gagtttgcct gaacgcagtt catcgcctaa ctcggttttc
     9061 tttttcgcga actgttcaac atcgcttgcc agcagcacat tgtggctgta atcgagcaga
     9121 ttcaggtagc ggtcaaagat tttggccgaa aatgcctgat cgaggtcgaa ctggcgataa
     9181 tgagaacggg tgaagcgcga cgttacgcgc tcacttaccg tcgcatgctg cgtctcttcc
     9241 tttaataccg gaatttgatc agcacgcgtg atatcttcta cagcgaaggt ctggcctgct
     9301 attgcaagca ggccagctaa cgcggtaagc ctaaaaaaca tgttcatgcc tggcccggcc
     9361 tccgtttcag aacaccaggt gttctgcgcg cacaatcaaa gacatacccg aattcagctg
     9421 gacgcggacg ccgtctttgg tgatttctaa tacggtggca tccatcgcgt tttgacccgc
     9481 tttcaccttc agggcttgtc cgacagtcag agctgaaatg tcagaaaccg gggtgtgctg
     9541 ttcttcgcga ggtgctttta ctgtttttgg cgctttctct accggctttt gcgcacgagg
     9601 tttacgttca gcgccttctt tgcggcgtgg cgtggtcgga cgtggcttgc gttcgcggcg
     9661 cggtgcgtct tctttctcac cagcagctgc ggcagcttcg cgttttttcg cttgctgttc
     9721 agcacgctgt gcctgaacac gcgctttcgc ttcttcaagc tgcttgcgag catgctctac
     9781 atgttgctcg tccagctcac cgcatgggtt gccgtcaaga tcgacacgcg ttgcgccggg
     9841 tttaacaccg taaagataac gccagctcga agtgtagaga cgtaaagcgg atcgtaattg
     9901 cgttttgctc aggttcattt ccccggcaac acgatcgacc aaatcctgaa aaataccaat
     9961 tttcagcgga cgcgcttcac cttccgcact gaaacagtgg ggaaaacgtt cggccagaaa
    10021 cgcgattact tctttactgc tattcaactt aggttgattt tccatgaaat ttcctgatta
    10081 caacggacgt agccaacaag cgcaggcatg aacaggcgtc attataatga cgctaccagt
    10141 aaatgctacg ttatccgttg attatcctgc gacgctcgca aagaattttt tgtaatccgt
    10201 cgttgcaagc actttttcaa gctgtgccac aagctgacgt aagccttgct cgtcctcgtc
    10261 tgtaaagcga gcgaagacag tgctatcgat gtcgagaaca ccaataatct gatttttcac
    10321 caccagcggc agaacaattt cagaattact cgccgcatca caggcaatat gcccgtcaaa
    10381 cgcgtgaaca tcctcgatac gctgcacttg attgcgggca accgctgtgc cgcacacccc
    10441 gcgcccgacg ggtatccgga cacaggcaat tttgccctga aatggtccga gtaccagtgt
    10501 atcgtcctca agcaaataaa aacctgccca gttaacgtca gtgagacgct cgtataacaa
    10561 cgcactggtg ttcgcaagcg ttgccagaaa actggtttct cccgccatca gcgcgttaaa
    10621 gtcgcgattt aaatccgcgt aaaattctgt tttgttcatt acttaatcac ttggttgtct
    10681 tacctggaac tgccagccta ttaaaataag cattaaatgc gttaatgctc aagatcattc
    10741 ccatcatggg ttaagattaa tgttaattct tattacattt ggcacgtcat ggctcttaac
    10801 acaccacaaa ttacgccgac aaaaaagata acagtgaggg caatcggcga ggaactgccg
    10861 cgtggtgatt accaacgttg cccgcaatgt gacatgctgt ttagcctgcc cgagataaat
    10921 tctcatcaaa gtgcctattg tccgcgctgt caggcaaaaa ttcgcgacgg gcgcgactgg
    10981 tcgctaacgc gcctggcggc aatggccttc accatgctgt tgcttatgcc gtttgcctgg
    11041 ggcgaaccgc tgttgcatat ctggctgtta ggcattcgta ttgacgccaa cgtcatgcaa
    11101 ggcatctggc aaatgaccaa acagggcgat gcgataacgg gatcaatggt ctttttctgc
    11161 gttattggtg cccccctcat tctggtgtcc tccatagcat atttatggtt tggtaaccga
    11221 ctgggaatga atttacgtcc ggtactgctg atgcttgagc gacttaaaga gtgggtaatg
    11281 ctggatatct acctggtcgg cattggcgtt gcttctataa aggtacagga ttatgcccat
    11341 atccaggcgg gtgtcggctt gttctctttt gtggcgttgg tgattttaac gacggtgacg
    11401 ttgtcacatc ttaatgttga ggagctgtgg gagcgatatt atccgcagcg ccccgctacg
    11461 cgtagggacg agaaacttcg tgtctgtctt gggtgccatt ttaccggcta tcctgatcag
    11521 cgtggtctct gcccgcgttg ccatatcccg ctacgcctgc gtcgtcgtca tagtttgcaa
    11581 aaatgctggg cggcgctgtt agcgtcaatc gttttgttgt taccagccaa cctattgcct
    11641 atttctatca tttatctgaa tggtggccgg caggaagata cgattctttc cggaattatg
    11701 tcgctggcaa gtagcaacat tgcggttgcg ggaatcgtgt ttatcgccag tattctggta
    11761 ccgtttacta aagtgatcgt catgttcact ttactgttga gcattcattt taaatgccag
    11821 caaggtttac gcacacgcat tctgttactg cggatggtga cctggattgg acgctggtcg
    11881 atactcgacc tgtttgtcat atctttaacc atgtcgctga ttaatcgcga tcagatcctc
    11941 gcttttacta tgggaccggc tgcgttttat tttggcgctg ccgtaatttt gactattctt
    12001 gctgtggaat ggctggacag ccgcttactt tgggatgcac atgagtcagg aaacgcccgc
    12061 ttcgacgact gaagcgcaga ttaaaaataa acgccgtatc tcacctttct ggctgctgcc
    12121 tttcatcgcg ctaatgattg ccggttggct gatttgggac agttatcagg accggggtaa
    12181 taccgtcacc atcgacttta tgtcggcgga tggtattgtt ccgggccgta cgcctgttcg
    12241 ttatcagggc gttgaagtcg gaacagtgca ggatatcagc ctcagcgacg atcttcgtaa
    12301 gattgaagtc aaggtcagca tcaagtccga tatgaaagat gcgctgcgcg aagagactca
    12361 gttctggctg atgacgccaa aagcatcgtt ggcaggtgtc tccgggctgg acgccctcgt
    12421 cggtgggaac tatatcggca tgatgccggg taaaggtaaa gagcaggatc actttgtcgc
    12481 gctcgatacc caaccgaaat atcggctgga caatggcgat ctgatgatcc acctgcaagc
    12541 ccccgctctc ggttcgctga acagcggttc attggtctat ttccgcaaga tcccggtggg
    12601 aaaagtctac gactatgcca tcaatcccaa caagcaaggc gtggtgattg atgtcctgat
    12661 cgagcggcgt tttaccgatc tggtgaaaaa aggtagccgt ttctgaaacg tttccggcgt
    12721 tgatgccaac gtcagtatca gtggcgcgaa ggtgaaactg gaaagtctgg cggcactggt
    12781 taacggtgcg attgccttcg attcacctga agagtcgaaa cctgccgagg cggaagatac
    12841 ctttggtctg tatgaagatc tggcccacag ccagcgtgga gtaataataa aactggaact
    12901 gccgagtgga gcaggattaa ccgccgactc gacgccgtta atgtatcagg ggctggaagt
    12961 cggacagctg actaaactgg atttaaatcc tggtggtaaa gtcactgggg aaatgaccgt
    13021 tgatccctgc ttcgtgaaaa tacccgcatc gaattacgca acccgaaatt atcccttagc
    13081 gatgctaatc tcagcgccct gctgaccgga aaaacctttg agttggtgcc cggcgatggc
    13141 gagccacgca aagagttcgt tgttgtgccg ggcgaaaaag ccctgctgca agagccggac
    13201 gttctgacac tgaccctgac agcaccggaa agttacggta ttgatgcggg tcagccgctc
    13261 attcttcacg gcgtgcaggt aggccaggtt atcgatcgta aactcaccag caaaggcgtc
    13321 acctttaccg tcgccatcga gcctcagcat cgagaactgg taaaaggcga tagcaaattt
    13381 gtcgtcaaca gccgtgtcga cgtgaaggtg gggctggatg gcgttgagtt tctcggtgcc
    13441 agcgcctcag aatggattaa cggcgggata cgtattctgc cgggcgataa aggcgagatg
    13501 aaagccagct atccactgta tgccaatctg gaaaaagcgc tggagaacag ccttagcgat
    13561 ttacccacca caaccatgag tttgagtgca gagacgctgc cggatgtgca ggcaggatcg
    13621 gtagtgctgt accgtaaatt tgaagttggt gaagttatta ccgtgcgtcc gcgagctaac
    13681 gcgttcgata tcgatctgca cattaaaccg gaatatcgca accttttgac cagcaacagc
    13741 gtgttctggg cagaaggcgg ggcgaaagtt cagctgaatg gtagtggcct gaccgtacag
    13801 gcatccccgc tctccagagc attaaagggg gccattagct tcgataacct cagtggtgcc
    13861 agcgccagtc agcgtaaagg cgacaagcga attctgtatg cttccgaaac agcggcccgt
    13921 gcggttggca ggcagattac gcttcacgct ttcgatgccg gaaaactggc ggtcgggatg
    13981 ccaattcgct atctcggtat tgatatcggg caaatccaga cgctggatct gattaccgca
    14041 cgcaatgaag tacaggcaaa ggcggtgctc tatccggaat atgtccagac ctttgctcgc
    14101 ggtggtacgc gcttctcagt ggtcacaccg caaatctcgg cagctggcgt tgagcatcta
    14161 gatactatcc tccagccgta tatcaacgtc gaaccaggcc gtggcaatcc tcgtcgcgac
    14221 tttgaattag aagaggccac cattactgat tcgcgttacc tagatggttt aagcattatt
    14281 gttgaagcgc cggaagccgg ttcgttaggc atcggtacgc ctgtgctgtt ccgtggtctg
    14341 gaagtaggta cggttaccgg aatgacgctg gggacattgt cagatcgcgt gatgattgcg
    14401 atgcgcatca gtaaacgcta tcaacacctg gtgcgtaaca attccgtctt ctggttggca
    14461 tcgggttaca gtctggactt tggtctgacg ggcggcgtag tgaaaaccgg cacctttaac
    14521 caatttatcc gtggcggcat cgccttcgcc acgcctccgg gtacgccact tgcaccgaaa
    14581 gcccaagaag gcaaacactt cctgttgcag gaaagtgaac cgaaagagtg gcgtgaatgg
    14641 ggaactgcgc ttcccaaata atgcccactg ctccggcgtg cctgcgccgg agcgcttatg
    14701 ctaaactgcg cgcctgtttt tttgccagtg gtacatgctc gtggcccaac acaccgttta
    14761 tttcccggac gcgtttctga cacaaatgcg cgaagctatg ccttcgacgc tctcatttga
    14821 tgattttctt gccgcctgtc agcgcccgtt gcgccgcagc attcgcgtta atacgctgaa
    14881 aatctccgtt gctgatttcc tgcaattaac cgctccttat ggctggacgc ttacgccaat
    14941 tccgtggtgt gaagaaggtt tctggattga acgcgacaat gaagatgcat tgccattggg
    15001 tagtaccacc gagcatttaa gtggcctgtt ttatattcag gaagccagtt caatgttgcc
    15061 cgtcgccgcc ttgtttgctg acggtaatgc accacagcgg gtgatggatg tcgctgctgc
    15121 gccaggctca aaaacgacgc aaattgccgc gcggatgaat aacgaagggg caatccttgc
    15181 caatgagttt tccgccagtt gggtaaaagt gttacatgcc aatatcagcc gctgtggcat
    15241 cagtaatgtt gcgctcacac attttggtgg ccgcgtgttt ggtgcggcag tgccagaaat
    15301 gtttgatgcc attttgctgg acgctccctg ctccggcgaa ggcgtggtgc gtaaagatcc
    15361 cgatgcgcta aaaaactggt cacccgaaag caatcaggaa atcgcagcta cccaacggga
    15421 actgattgac agcgcctttc atgcattacg tcctggcggt acgctggttt actcgaccta
    15481 taccttaaac cgggaagaaa acgaagccgt ttgccggtgg ctgaaagaga cttaccccga
    15541 cgaagtagag tttttaccac ttggcgatct cttccctggt gcaaacaagg cgctgaccga
    15601 agaaggtttt ttgcatgttt tcccacaaat ttacgactgc gaaggcttct tcgttgctcg
    15661 tctgcgtaaa actcaggcga tccccgcctt acccgcccca aaatacaaag tgggcaattt
    15721 cccgttcagc ccggtgaaag atcgcgaagc cggacaaat
//
LOCUS       DAZSNM010000086        15524 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZSNM010000086 DAZSNM010000000
VERSION     DAZSNM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15524
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.086"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..73)
                     /locus_tag="ACHW42_002243"
     CDS             complement(<1..73)
                     /locus_tag="ACHW42_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HFR8944053.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="ACHW42_002244"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="ACHW42_002244"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR8944054.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="ACHW42_002245"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="ACHW42_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR8944055.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="ACHW42_002246"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="ACHW42_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR8944056.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="ACHW42_002247"
     CDS             2703..2939
                     /locus_tag="ACHW42_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR8944057.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="ACHW42_002248"
     CDS             2942..3799
                     /locus_tag="ACHW42_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR8944058.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5811
                     /locus_tag="ACHW42_002249"
                     /pseudo
     CDS             3799..5811
                     /locus_tag="ACHW42_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="ACHW42_002250"
     CDS             complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="ACHW42_002250"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR8944059.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6414..7310)
                     /locus_tag="ACHW42_002251"
     CDS             complement(6414..7310)
                     /locus_tag="ACHW42_002251"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR8944060.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7359..7598)
                     /locus_tag="ACHW42_002252"
     CDS             complement(7359..7598)
                     /locus_tag="ACHW42_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR8944061.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7701..8300
                     /gene="nemR"
                     /locus_tag="ACHW42_002253"
     CDS             7701..8300
                     /gene="nemR"
                     /locus_tag="ACHW42_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR8944062.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8337..9434
                     /gene="nemA"
                     /locus_tag="ACHW42_002254"
     CDS             8337..9434
                     /gene="nemA"
                     /locus_tag="ACHW42_002254"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR8944063.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9515..9922
                     /gene="gloA"
                     /locus_tag="ACHW42_002255"
     CDS             9515..9922
                     /gene="gloA"
                     /locus_tag="ACHW42_002255"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR8944064.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10025..10672
                     /gene="rnt"
                     /locus_tag="ACHW42_002256"
     CDS             10025..10672
                     /gene="rnt"
                     /locus_tag="ACHW42_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR8944065.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10765..15223
                     /locus_tag="ACHW42_002257"
                     /pseudo
     CDS             10765..15223
                     /locus_tag="ACHW42_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15267..>15524)
                     /locus_tag="ACHW42_002258"
     CDS             complement(15267..>15524)
                     /locus_tag="ACHW42_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR8944066.1"
                     /translation="SGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTAN
                     ADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3559 a         4109 c         4079 g         3777 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccagctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gtaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tactttcgct attcagcttt gtggtcaaat ttggcctgat ggtccagatt
     5101 agcgatctgt ggcaattttt actgtttctc tttccactgc tggcgacaat gcagcttctt
     5161 aaattgcaga tgcccaaatt tgccgcactg tgggggcaac tgattgtttt tatgggttcg
     5221 tttatcgctg tcactaatcc cccggtgtat gattttgctg attttcttaa ctataatctg
     5281 gcaaaaatcg ttggcgttgc gctggcttgg ctagcgttcg ccattctgcg tccaggatcg
     5341 gatgctcgta aaagccgccg ccatattcgc gcgctgcgcc gggattttgt cgatcagcta
     5401 agccgccatc caacactgag tgaaagcgaa tttgaatcgc tcacttatca tcacgtcagt
     5461 cagttgagta acagccagga tgcgctggct cgccgttggt tattacgctg gggtgtagtg
     5521 ctgctgaact gttctcatgt tgtctggcaa ttgcgcgact gggaatcgcg ttccgatccg
     5581 ttatcgcgag tacgggataa ctgtatttca ctgttgcggg gagtgatgag tgagcgtggc
     5641 gttcagcaaa aatcactggc ggccacactt gaagaattac agcggatttg cgacagcctt
     5701 gcccgtcatc atcaacctgc cgcccgtgag ctggcggcaa ttgtctggcg gctgtactgc
     5761 tcgctttcgc aacttgagca agcaccaccg caaggtacgc tggcctcgta attacttaat
     5821 tacaccacag gcatagcgtt caccgccacc gcccagcggt ttaggttgat cggacatatt
     5881 atcgccgcca acgtggacca tcagcgcctt gtctttgatt tcatccagtg atttcagacg
     5941 aggcgcgatg acggcatcgg tagctttgcc gtcattattg acgaccagtg caggcagatc
     6001 gcctaaatgc ccggtacctt ctggcccttc atgtttaccg gtattttgtg gatcaagatg
     6061 cccgcctgcg gattccgcgg cgctggcttt gccatctttg gtggctggct ggcagcttcc
     6121 tttggcatga atatggaagc catgttcacc ggggggtaat gctttcagat cgggtgaaaa
     6181 ctccagacct ttatcggttt cagtaatggt gacgctacca attgactgcc caaccccttg
     6241 cgacgtgacg aggttcatct cgactttttc actggcagct tgtgcgccgg tcgcaacaac
     6301 cagcgccaga atggccagac taaaacgttt cataggacct ccgttcattg tgcattcctg
     6361 aataagtgta aaccagtgac acatttttga acggcggcta ttcctaaaag tgcttacggt
     6421 acgtcgtacc ccagtgcagc tttacggata cgaaaccatt gttgacgggt cattttcagt
     6481 gtttctgctt cgacagctgc ccgtacgcgc tcaattttac ctgagccgat aattggcagc
     6541 ggctgcgacg gtaaacgtaa tacccaggcg taaaccacct gttcaatcga gcccgcgttt
     6601 aactcctccg ccaccacagc cagttcatca cgcagcggct ggaaataatc atcattaaac
     6661 agacgaccac caccaaggca ggaccacgcc atcggacgaa cacgcagttg ttgtagttgg
     6721 tcgagcgtgc catccagcag taacggctga tgcaccgggg atatttccac ctgattagtg
     6781 gcaagggtaa acggcagacg tgattgcaac agggcaaatt gcgcaggcgt aaagttcgat
     6841 acgccaaaat gacgcacttt gccgctctga tgcagatgtt tgaacgcgtc cgccacttca
     6901 tcggcatcca ttaacgggtc tggtcggtgg attaacagca gatccagatg atcggtcgcg
     6961 agattaatta gcgactgttc ggcgctctta atgatgtgat tgcggtcagt gatgtaatga
     7021 ccaatgacgt tttcttcacg cgcggtcgtc gcgataccgc atttactgac gatttccatc
     7081 cgttcacgca ggtgaggtgc cagtttcagt gcctcgccaa acgccgcttc gcactgatag
     7141 ccaccataaa tatcagcatg gtccacggtg gtcacgccga gatccagatg ctcttcaata
     7201 aaactgacca gctggcgggc ggacatattc cagtccatca atcgccagta gcccatcaca
     7261 aaacgggaaa actccgggcc ttccggcgca atagtaatac gctgaaccat aatcgcttcc
     7321 tcttatcaga tataagagga gtatacgcaa gattaggttc aaaagagtga tggttgctcc
     7381 ggttcgtctg atgacgctgg cttatttgcg cgtaatttgc gcattaatcg ctgccgacaa
     7441 aggcgcagca cctcttgttt ttcgccatcg ctcattttat tccagttaaa acgctcatcc
     7501 cgactacgaa aacagccgcg acaaaacccg cgttcgtcag actggcaaat tccccggcac
     7561 gggctctgga cgggaaagaa ctctaattgc tccgccacct cgccctcctc agataagatt
     7621 attaccatta ttgaagctgt taatgtccaa agtagcaact ttgcttgcac tagaccaact
     7681 ggtctactac actccaacgc atgaacaaac acaccgaaca tgatactcgc gaacatctct
     7741 tggcgacggg cgagcaactt tgcctgcaac gtggattcac cgggatgggg ctaagcgaat
     7801 tactaaaaac cgctgaagtg ccgaaagggt ccttctatca ctactttcgc tctaaagaag
     7861 cgtttggcgt tgccatgctt gagcgtcatt acgccgcata tcaccagcga ctggctgagt
     7921 tgctgcaatc cggcgaaggt aactaccgcg accgcatact ggcttattac cagcaaacac
     7981 tgaaccagtt ttgccaacat ggaaccatca gtggttgcct gacagtaaaa ctctctgccg
     8041 aagtgtgcga tctgtcagaa gatatgcgca gcgcgatgga taaaggtgct cgcggcgtga
     8101 tcgccctgct ctcgcaggct ctggaaaatg gccgtgagaa ccattgttta accttttgtg
     8161 gcgaaccgct acaacaggca caagtgcttt acgcactatg gcttggcgcg aatctgcagg
     8221 ccaaaatttc gcgcagtttc gagccactgg aaaacgcgct ggcccatgta aaaaacatta
     8281 ttgcgacgcc tgccgtttag caggcatttt ttatcaccag acgaccggga gcctttatgt
     8341 catctgaaaa actgtattcc ccactgaaag tgggcgcgat cacggcggca aaccgtattt
     8401 ttatggcacc gctgacgcgt ctgcgcagta ttgaaccggg tgacattcct accccgttga
     8461 tggcggaata ctatcgccaa cgtgccagtg ccggtttgat tattagtgaa gccacgcaaa
     8521 tttctgccca ggcaaaagga tatgcaggtg cgcctggcat ccatagtccg gagcaaattg
     8581 ccgcatggaa aaaaatcacc gctggcgttc atgctgaaaa tggtcatatg gccttgcagc
     8641 tgtggcacac cggacgcatt tctcacgcca gcctgcaacc tggcggtcag gcaccggtag
     8701 cgccttcagc acttagcgcg ggaacacgta cttctctgcg cgatgaaaat ggtcaggcga
     8761 tccgcgttga aacatccatg ccgcgtgcgc ttgaactgga agagattccg ggtatcgtca
     8821 atgatttccg tcaggccatt gctaacgcgc gtgaagccgc ttttgatctg gtagagctcc
     8881 actctgctca cggttatttg ctgcatcagt tcctttcccc ttcttcaaac catcgtaccg
     8941 atcagtacgg cggcagcgtg gaaaatcgcg cacgtttggt actggaagtg gtcgatgccg
     9001 ggattgaaga atggggtgcc gatcgcattg gcattcgcgt ttcgccaatc ggtactttcc
     9061 agaacacaga taacggcccg aatgaagaag ccgatgcact gtatctgatt gaacaactgg
     9121 gtaaacgcgg cattgcttat ctgcatatgt cagaaccaga ttgggcgggg ggtgaaccgt
     9181 atactgatgc gttccgcgaa aaagtacgcg cccgtttcca cggtccgatt atcggcgcag
     9241 gtgcatacac agtagaaaaa gctgaaacgc tgatcggcaa agggttaatt gatgcggtgg
     9301 catttggtcg tgactggatt gcgaacccgg atctggtcgc ccgcttgcag ctcaaagctg
     9361 agcttaaccc acagcgtgcc gaaagtttct acggtggcgg cgcggaaggc tataccgatt
     9421 acccgacgtt gtaatccaac attgcgagcg gcgtaaagcc gccgctatac taaaacaaca
     9481 ttttgaatct gttagccatt ttgaggataa aaagatgcgt cttcttcata ccatgctgcg
     9541 cgttggcgat ttgcaacgct ccatcgattt ttataccaaa gtgctgggca tgaaactgct
     9601 gcgtaccagc gaaaacccgg aatacaaata ctcactggcg tttgttggct acggcccgga
     9661 aaccgaagaa gcggtgattg aactgaccta caactggggc gtggataaat acgaactcgg
     9721 cactgcttat ggtcacatcg cgcttagcgt agataacgcc gctgaagcgt gcgaaaaaat
     9781 ccgtcaaaac gggggtaacg tgacccgtga agcgggtccg gtaaaaggcg gtactacggt
     9841 tatcgcgttt gtggaagatc cggacggtta caaaattgag ttaatcgaag agaaagacgc
     9901 cggtcgcggt ctaggcaact aatctcctgc cgggcgtgaa ctcatcgcgc ccgcatcttt
     9961 actgcatcga caagtaatat ttgtcataat gcgcgctgca atttatccgt attaagagaa
    10021 tcagatgtcc gataacgctc aacttaccgg tctgtgcgac cgttttcgtg gtttttatcc
    10081 tgttgtgatc gatgttgaaa cagccggatt taacgccaaa accgatgcgc tgcttgagat
    10141 tgccgccatc accctgaaaa tggatgaaca aggctggctg atgccggaca ccacattaca
    10201 tttccacgtc gaaccatttg tcggcgcaaa tttgcaacca gaagccctcg ccttcaacgg
    10261 cattgacccg aacgatcccg atcgcggcgc ggtcagcgaa tacgaggcgc tgcacgaaat
    10321 ttttaaagtt gtacgtaaag gtattaaagc gagcggctgt aaccgcgcca ttatggtggc
    10381 gcacaacgcc aattttgatc acagctttat gatggccgcc gcagaacgcg cctcgctgaa
    10441 acgtaacccg ttccaccctt tcgccacttt tgacactgct gcactggccg ggctggcact
    10501 cggacaaacc gtattgtcaa aggcttgcca gaccgctggc atggacttcg acagcaccca
    10561 ggcgcactcc gcgctgtacg acaccgaacg cactgctgtg ctgttttgtg aaatcgtcaa
    10621 ccgctggaaa cgtctgggag gctggccgct acctgccgcc gaagaggtgt aatcgagtcg
    10681 atgcctgatg acatgcaatg attcaggcat ctatagtgag gctattccac gcatcaagca
    10741 tgatattcac ggggaatagc gttaatggca gataatccag acccttcatc gctcctgccg
    10801 gacgtgtttt caccggcgac ccgcgactgg tttcttcgcg cctttaaaca gccgaccgct
    10861 gtccagtcgc aaacctggca tgtggcggcg cgaggcgaac atgcgctggt gattgcaccg
    10921 actggctccg ggaaaacgct ggcagcattt ctctacgccc tcgatcggct cttccgcgaa
    10981 ggcggcgaag atacccgcga ggcgcataag cgtaaaacct cacgcatcct ctatatttca
    11041 ccgataaaag ccctgggcac cgacgttcag cgcaacttgc agatcccgtt gaagggtatt
    11101 gccgatgaac ggcggcggcg cggcgaaacg gtaattattg atgaagtcca cgcggtagcg
    11161 ggcagtaaac gtggtgcgca tctggcgtta agtctggagc ggctcgatgc gctgctccac
    11221 acctcagcac agcgaattgg cctttctgcc actgtgcgct cagccagcga tgtggcagca
    11281 tttcttggtg gcgatcgccc ggttacggta gtcaacccgc ccacaatgcg ccatccgcag
    11341 atacgaattg tcgtaccggt cgccaatatg gatgatgtct catcggtcgc cagcggcacc
    11401 ggcgaagaca gccatgccgg ccgggaaggc tccatctggc catatattga aacgggtatc
    11461 cttgatgaag tgttgcgcca tcgctcgacc attgtcttta ctaattcacg cgggctggcg
    11521 gaaaaactga cggcacgatt aaatgagctt tacgccgcac gcttacagcg ttccccgtct
    11581 atcgccgttg atgcggccca tttcgagtcg acctccggcg caacctctaa ccgtgtacaa
    11641 agtagcgacg tttttattgc ccgctcacac cacggctccg tctctaaaga acaacgagca
    11701 atcaccgaac aggcgctgaa atcgggtgaa ttacgctgcg tggtcgcaac ctccagtctt
    11761 gaactgggga ttgatatggg cgcggtggat ctggtgattc aggtggcaac gccgctttct
    11821 gttgccagtg ggttacaacg cattggtcgc gccggacatc aggttggcgg tgtatctaaa
    11881 gggctgtttt tcccccgtac ccggcgtgat ttagtcgatt ccgcagtcat tgtagagtgt
    11941 atgttcgcag gcaggctgga aaacctgaca ccaccgcata atcctctcga cgtccttgcg
    12001 cagcaaaccg ttgccgccgc ggcgatggat gcattacagg tagacgaatg gtactcccgc
    12061 gtacgccgta ccgcaccgtg gaaagatctg ccaagacgtg tttttgacgc cacgctggat
    12121 atgctttccg ggcgctatcc ctctggcgat ttttctgctt ttcgccccaa actggtctgg
    12181 aacagggaga ccgggatatt gaccgcccga cctggcgctc aattgttggc ggttaccagc
    12241 ggcggcacca ttccggatcg tggcatgtat agcgtgttat tacccgaagg tgaagaaaag
    12301 gccggttcgc ggcgggtggg tgaactggat gaggagatgg tatatgagtc gcgggtgaac
    12361 gacattatca ctctcggcgc tacctcatgg cggatccagc aaatcacccg cgatcaggtg
    12421 attgtgactc ctgctccggg tcgttctgcc cagctcccct tctggcgtgg tgaaggtaac
    12481 ggacgtccgg ctgaattagg cgagatgatc ggcgattttc ttcatttact ggcggatggc
    12541 gcgttctttt ccgggactat tcccccgtgg ctggcagaag aaaatacgaa cgccaatatt
    12601 caggggttga ttgacgagca acgcaacgcg acgggcatcg ttccggggag tcgccatctg
    12661 gtcctcgaac ggtgccgtga tgaaattggc gactggcgta ttattttgca ctctccctat
    12721 ggaagacggg tgcatgaacc ctgggcgctg gcgattgccg ggcgaataca tgccctatgg
    12781 ggcgctgacg cgtctgtggt cgccagtgat gacagcattg ttgcgcgcat tcctgacact
    12841 gatggtaaat tgcccgacgc cgcgattttt ttgttttaac cagaaaagtt gctgcaaatt
    12901 gtccgcgagg cggtaggcag ctcggcactt ttcgccgccc gttttcgcga atgcgccgcg
    12961 cgggcattat taatgccggg ccgcacgccg ggccatcgca ccccgctttg gcaacaacgg
    13021 ctgcgcgcca gtcagttgct ggaaatcgct cagggatatc cggattttcc ggtcattctc
    13081 gaaaccctac gcgaatgtct gcaagatgtt tatgatcttc ccgcactgga acgtttgatg
    13141 cgtcgcctga acggtggcga aattcaaata tccgatgtaa cgaccactac gccctcgcct
    13201 ttcgccacaa gtttattgtt cggctatgtc gcggaattta tgtaccagag cgacgccccg
    13261 ctggcagagc gccgggcatc cgtactgtcg ctggacagcg agttactgcg caatctactc
    13321 ggacaggtcg atccggggga attactcgac ccgcaggtca ttcgccaggt ggaagaagag
    13381 ttgcaacgac tggctcctgg tagaagagcg aaaggtgaag aaggattgtt cgacctgctg
    13441 cgcgaactgg ggccaatgac cgttgaagac ctggcgcaac ggcatacagg cagcagtgaa
    13501 gaggttgcgt cgtatctgga aaatcttctt gcagtaaaac gaatcttccc agcgatgatt
    13561 agcggacagg agcgtcttgc ctgtatggat gatgccgcca ggctgcgtga tgccctcggc
    13621 gtacggctac cagagtcatt gccagagatt tatttacata gagtcagtta cccgcttcgc
    13681 gacctctttc tgcgctatct ccgggctcat gctctggtca cggctgaaca actggctcat
    13741 gagtttagtc tcggtattgc cattgtcgaa gagcagcttc agcaactgcg tgaacagggt
    13801 ctggtgatga atctgcaaca agacatctgg gtgagcgatg aagtatttcg tcgtctgcgt
    13861 ttgcgctcgc tgcaagccgc cagagaagcg acgcgtcccg ttgcagccac gacctatgcg
    13921 cgattgctgc tggaacgtca gggcgtatta cccgccaccg atggtagccc ggcgctcttt
    13981 gcctcaacat ctccaggcgt ttatgagggc gtagatggag tgatgcgggt gatcgaacag
    14041 cttgccggag tcggtttacc cgcctcactc tgggaaagcc agatcctgcc tgcccgcgta
    14101 cgcgactatt cgccagaaat gctcgatgaa ttactggcaa ccggagcggt tatctggtcg
    14161 ggacaaaaaa aagctgggtg aagatgacgg cctggtggca ctgcatctac agaaatatgc
    14221 cgcagaatcg ttcactcccg ccgaagcgga tcaggcgaat cgttcggcgc tgcagcaagc
    14281 gataatcgct gttctggctg acggaggagc ctggtttgca caacaaatca gccaacggat
    14341 acgcgataaa atcgtcgaat cggttgattc ctcggcactg caagaggcgt tatgggcgct
    14401 ggtctggcaa ggcgtcatta ccagcgatat ttgggcaccg ttacgcgccc tcacccgcag
    14461 cagttccaac gcacgcacct caactcgccg cagtcaccgg gctcgtcgtg ggcgtcctgt
    14521 ctatgcacaa cccgtctcgc cgcgggtatc ttacaacaca ccaaatctgg ctggacgctg
    14581 gtcgttattg caggtggagc cactaaacga taccgaaagg atgctggcgc tggcggaaaa
    14641 tatgctcgac cgctacggca tcatcagtcg tcaggcggtg atagccgaaa atatccctgg
    14701 cgggtttcca tcgatgcaaa cactttgtcg aagtatggaa gactccgggc gaattatgcg
    14761 aggtcgtttt gtagaaggtc tgggtggcgc gcaattcgct gaacgtctga ctattgaccg
    14821 attgcgcgat ctggcgacac aagccacgca aacacgccac tatacaccag tggcgctctc
    14881 cgccaacgat ccggctaatg tgtggggaaa tcttctgccc tgggctgcac atccggcaac
    14941 gctggtacca acgcgtcggg cgggtgcgct ggtggtcgtt tctggcggca aattgttact
    15001 ctatctggcg caaggcggca aaaaaatgct ggtctggcag gaaaaggagg aattactcgc
    15061 cccagagatt ttccaggcgc tgaccaccgc actgcgtcgc gaaccaaggc tgcgctttac
    15121 gctaacagaa gtgaatgatc ttccggtccg gcaaacgccg atgtttacgc tgctgcgtga
    15181 ggcgggattt tcaagttcgc cacaagggct ggattgggga tagttgacat cctccacgcc
    15241 ctgaaagacg tggttccctg ctacgttcaa gctgtcgcct gaatcatttc ggtgggttcc
    15301 tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc gtgccccgcc
    15361 gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg tatatccaca tacctggcat
    15421 ctgaatttac tttgtgacag gcggttttct ttcgctgtat gaccacagca cgcgcaacgc
    15481 tggcttgtgt acgctggcgg aacagcaagc acctgaccgc cact
//
LOCUS       DAZSNM010000087        15434 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZSNM010000087 DAZSNM010000000
VERSION     DAZSNM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15434
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.087"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..880
                     /gene="yieE"
                     /locus_tag="ACHW42_002259"
     CDS             <1..880
                     /gene="yieE"
                     /locus_tag="ACHW42_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR8944067.1"
                     /translation="HITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQL
                     GVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNYI
                     LPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLED
                     ELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMELG
                     NSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSN
                     ALRRFLDYCDPANVPR"
     gene            1086..2555
                     /gene="lysP"
                     /locus_tag="ACHW42_002260"
     CDS             1086..2555
                     /gene="lysP"
                     /locus_tag="ACHW42_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR8944068.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2847..4838
                     /gene="cirA"
                     /locus_tag="ACHW42_002261"
                     /pseudo
     CDS             2847..4838
                     /gene="cirA"
                     /locus_tag="ACHW42_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(4870..5705)
                     /gene="yeiG"
                     /locus_tag="ACHW42_002262"
                     /pseudo
     CDS             complement(4870..5705)
                     /gene="yeiG"
                     /locus_tag="ACHW42_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            5963..6631
                     /gene="folE"
                     /locus_tag="ACHW42_002263"
     CDS             5963..6631
                     /gene="folE"
                     /locus_tag="ACHW42_002263"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR8944069.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6648..7724
                     /gene="yeiB"
                     /locus_tag="ACHW42_002264"
     CDS             6648..7724
                     /gene="yeiB"
                     /locus_tag="ACHW42_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR8944070.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            7866..8906
                     /gene="galS"
                     /locus_tag="ACHW42_002265"
     CDS             7866..8906
                     /gene="galS"
                     /locus_tag="ACHW42_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR8944071.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            9186..10184
                     /gene="mglB"
                     /locus_tag="ACHW42_002266"
     CDS             9186..10184
                     /gene="mglB"
                     /locus_tag="ACHW42_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR8944072.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10245..11765
                     /gene="mglA"
                     /locus_tag="ACHW42_002267"
     CDS             10245..11765
                     /gene="mglA"
                     /locus_tag="ACHW42_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR8944073.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11781..12791
                     /gene="mglC"
                     /locus_tag="ACHW42_002268"
     CDS             11781..12791
                     /gene="mglC"
                     /locus_tag="ACHW42_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR8944074.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(12827..14040)
                     /gene="preA"
                     /locus_tag="ACHW42_002269"
                     /pseudo
     CDS             complement(12827..14040)
                     /gene="preA"
                     /locus_tag="ACHW42_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(14034..15272)
                     /gene="preT"
                     /locus_tag="ACHW42_002270"
     CDS             complement(14034..15272)
                     /gene="preT"
                     /locus_tag="ACHW42_002270"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFR8944075.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT         3678 a         3778 c         4058 g         3920 t
ORIGIN      
        1 gcacatcacc ctccggcagt tggaagtttt tgcagaagta ttgaaaagtg gatcaaccac
       61 ccaggcgtcg gtgatgctgg cgttgtcgca atcagcagtg agcgcagcct tgactgacct
      121 ggaagggcag cttggcgttc aactgtttga tcgcgtgggg aaaagactgg ttgttaatga
      181 acacgggcgg ctgctctatc cgcgagcgtt ggcactgctt gaacaggcgg ttgaaatcga
      241 acaactgttt cgcgaagaca acggcgcgat tcgtatctat gccagtagta ccatcggtaa
      301 ctacattctg cctgcagtta tcgcccgtta tcgccatgat tatccgcagt tgccgattga
      361 acttagcgtt gggaacagcc aggacgtgat gcaagcggtg ctggatttcc gcgttgatat
      421 tggctttatt gaaggaccgt gccacagcac tgaaatcatt tctgaaccgt ggctggaaga
      481 cgagctggtg gttttcgccg cgccgacttc gccgttggct cgtggtccgg tcaccttaga
      541 acagctggcc gctgcgccgt ggatcctgcg tgaacgcggt tccggcacgc gggatattgt
      601 cgattatctg ttgctgtcac atttaccgaa gtttgagatg gcgatggaat taggtaactc
      661 cgaggcaatc aaacatgcgg tgcgtcatgg gttgggaatt agttgcctgt cgcgacgtgt
      721 gattgaagat caattgcagg caggcacatt aagtgaagtt gcggtccctc tgccgcgcct
      781 gatgcgtacg ttgtggcgta tacatcatcg gcaaaaacac ctttccaacg cgctacggcg
      841 ctttctggac tattgcgatc ccgcaaatgt gccgcgttaa gttgctgtac aagaacatgc
      901 tggtgctgtg tcgatttcgt gacgcagcgc cttcagcatg cattcgccag aaaagagatt
      961 ggctgcttta cttataatcc ctgggcgatc atgaaggtgt cttataaccg tgtatttctg
     1021 ccggaaggat tgccaatcgt ctgctacaat cgcgccatca tttttaagat ggatagcatt
     1081 tttgtatggg ttccgaaact aaaactacag aagcgccggg cttacgccgt gaattaaagg
     1141 cgcgtcacct gacgatgatt gccattggcg gttccatcgg tacaggtctt tttgttgcct
     1201 ctggcgcaac gatttctcag gcaggtccgg gcggggcatt gctctcgtat atgctgattg
     1261 gcctgatggt ttacttcctg atgaccagtc tcggtgaatt ggctgcatat atgccggttt
     1321 ccggttcgtt tgccacttac ggtcagaact atgttgaaga aggctttggc ttcgcgctgg
     1381 gctggaacta ctggtacaac tgggcggtga ctattgccgt tgacctggtt gcagcacagc
     1441 tggtcatgag ctggtggttc ccggatacac cgggctggat ctggagtgcg ttgttcctcg
     1501 gcgttatctt cctgttgaac tacatctctg ttcgcggctt tggtgaagcg gaatactggt
     1561 tctcactgat caaagtcacg acagttattg tctttatcat cgttggtgtg ctgatgatta
     1621 tcggtatctt caaaggcgcg cagcctgcgg gctggagcaa ctggacaatc ggcgaagcgc
     1681 cgtttgctgg tggttttgcg gcgatgatcg gcgtagctat gattgtcggc ttctctttcc
     1741 agggaaccga gctgatcggt attgctgcag gcgagtccga agatccggcg aaaaacattc
     1801 cacgcgcggt acgtcaggta ttctggcgaa tcctgttgtt ctatgtgttc gcgatcctga
     1861 ttatcagcct gattattccg tataccgatc cgagcctgct gcgtaacgat gttaaagaca
     1921 tcagcgtcag tccgttcacc ctggtgttcc agcacgcggg tctgctctct gcggcggcgg
     1981 tgatgaacgc agttattctg acggcggtgc tgtcagcagg taactccggt atgtatgcgt
     2041 ctactcgtat gctgtacacc ctggcgtgtg acggtaaagc gccgcgcatt ttcgctaaat
     2101 tgtcgcgtgg tggcgtgccg cgtaatgcgc tgtatgcgac gacggtgatt gccggtttgt
     2161 gcttcctgac ctccatgttt ggcaaccaga cggtatacct gtggctgctg aacacctccg
     2221 ggatgacggg ttttatcgcc tggctgggga ttgccattag ccactatcgc ttccgtcgcg
     2281 gttacgtatt gcagggacac gacattaacg atctgccgta ccgttcaggt ttcttcccac
     2341 tggggccgat cttcgcattc attctgtgtc tgattatcac tttgggccag aactacgaag
     2401 cgttcctgaa agataccatt gactggggcg gcgtagcggc aacgtatatt ggtatcccgc
     2461 tgttcctgat tatttggttc ggctacaagc tgattaaagg aactcacttc gtacgctaca
     2521 gcgaaatgaa gttcccgcag aacgataaga aataagtttc ctcccttcct tgctaagccc
     2581 tctcaaccga gagggctttt tcaattccat ttccctgaca aatcatgcgg atataaaatt
     2641 taacatttgg attgataatt gttatcgttt gcattatcgt tacgacgcaa tcaaaaaagg
     2701 ctgacaaatc agaggctgtt ccggctttct gggatgatca cctgcataca aataagtcca
     2761 ccgcgatgct gccgtacgca aggggacgta aagaagatgt gagcgatacc cattttattt
     2821 tcgtagttac ctcatggaga tatggaatgt ttaggttgaa ccctttcgta cgggtcgggc
     2881 tgtgtttgtc cgctatttct tgtgcatggc ctgtgttagc ggtcgatgat gatggcgaaa
     2941 cgatggttgt cactgcatct tccgtggaac aaaatcttaa agatgcaccc gccagtatca
     3001 gcgtcattac ccaggaagac ctgcagcgaa aaccggtaca gaatctgaag gatgtcctca
     3061 aagaagtgcc tggcgtacaa ctgacgaacg aaggggataa ccgtaagggc gttagtattc
     3121 gtggtctgga cagcagctac accctgattc ttgtcgacgg taaacgcgtt aactcccgca
     3181 atgccgtctt ccgccacaat gatttcgatc tgaactggat cccggtcgat tccatcgaac
     3241 gtattgaagt ggttcgtggc ccgatgtcgt cgctatacgg ctccgatgcg ctcggcggtg
     3301 tagtgaatat catcaccaaa aaaatcggtc agaaatggtc gggtaccgtt accgtcgata
     3361 ccaccattca ggaacatcgc gatcgcggtg atacctataa cggtcagttc tttaccagtg
     3421 gaccattaat tgatggtgtg ctgggaatga aagcttacgg cagcctggca aaacgtgaaa
     3481 aggatgaccc gcaaaactca acgaccaccg ataccggaga aacgccgcgt attgaaggat
     3541 tctccagccg cgacggcaat gtcgaatttg cctagacacc gaatcaaaat cacgatttta
     3601 ctgccggata cggtttcgac cgtcaggatc gtgattccga ttcgctggac aaaaaccgcc
     3661 tggaacgcca gaactactgc gtcagccata atgggcgttg ggattacggc accagcgaac
     3721 tgaaatccta cggtgagaaa gtcgagaaca aaaaccctgg caacagcagc ccgataactt
     3781 ccgaaagcaa tacggtcgac ggcaaataca cgttgccgct gacggcgatt aatcagtttc
     3841 tcacggttgg cggtgaatgg cgtcacgaca aacttagcga tgcggtgaac ctgaccgggg
     3901 gaaccagctc caaaacgtct gccagccagt acgcgctgtt tgtggaagat gaatggcgga
     3961 tcttcgagcc gctggcgctg acgaccggcg tgcgtatgga cgatcacgaa acctacggtg
     4021 aacactggag tccgcgtgcc tacatggttt ataacgccac cgacaccgta acggtgaaag
     4081 ggggctgggc gacggcattt aaagcacctt ctctgttgca acttagccct gactggacga
     4141 gcaattcctg ccgtggcgca tgtaagattg tgggtagccc ggatctgaaa ccagaaacca
     4201 gcgaaagttg ggagctgggg ctttactaca tgggtgaaga aggctggctg gaagggggtg
     4261 aatccagcgt tatcgttttc cgtaacgatg tgaaagatcg tatcagcatc agccgtacgt
     4321 ctgacgtcaa cgctgcaccg ggctaccaaa actttgttgg ttttgagacg ggcgctaacg
     4381 gacggcgcat accggtattt agctactaca gcgttaacaa agctcgtatt cagggcgtgg
     4441 aaaccgaact gaaaattccg ttcaacgatg aatggaaact gtcgatcaac tacacctaca
     4501 acgatggtcg tgatgtcagc aacggcgaaa acaaaccgct atccgatctg ccgttccata
     4561 ctgctaacgg tacgctggac tggaaaccgc tggcgctgga agactggtca ttctatgttt
     4621 ctggtcacta taccgggcag aaacgcgccg acagcgcgac ggctaaaaca ccgggcggtt
     4681 ataccatctg gaataccggc gcggcctggc aggtgactaa agacgtcaaa ctgcgcgcag
     4741 gcgtgctgaa ccttggcgac aaggatctca gtcgtgacga ctacagctat aacgaagacg
     4801 gacgtcgtta ctttatggca gtggattatc gcttctgatg agaagatgcc cggcgaaccg
     4861 ggcggacttt cacttcagta aatactgcgc atggaagcgc aggtgatcct ctataaaaga
     4921 ggcgatgaag tagtaactgt gatcatatcc cggctgaata cgcagcgtca tcggccacgc
     4981 tttctggcgc gcggcttctg ccaataccgc aggttgcaac tggtcggcaa gaaactgatc
     5041 attatcgccc tgatcgataa gcgtcgggat cgcatcctgc gcgttactgg catacatcag
     5101 cgcgcaacta tcccattcca gcatgcattt ttgtcctcac ctaaataggt gctaaacgct
     5161 ttgattcccc acgggacgct gcacggattc acaatgggcg caaaggccga aacgctggtg
     5221 tatttacccg gatttttcag cgccataatc agcgcaccgt gaccacccat tgagtgaccg
     5281 ctaatggcgc agcggtcgct gacattaaat tgcgactgaa ccagcgccgg taattcatcg
     5341 cgcagataat catacatccg gtaatgcgtc gcccacggcg gttgcgtggc attaagataa
     5401 aagcctgcgc cctggcctaa atcgtagcca tcgtcgttgg caaccttttc gccgcgcggg
     5461 ctggtgtctg gcatcaccag tacaatcccc agttccgccg ctacccgctg ggcacccgcc
     5521 ttggtggtga agttctcgtc attgcaggtt aatccggaaa gccagtacag cactggtggc
     5581 ggggtgtgat cacgaggggg ggggagaaag atactgaacg tcatcgggca gtttaaggta
     5641 ctggagtcgt gtcgccagcg ttgctgccag ccttcaaaac agcggtgctc ttcgagcatt
     5701 tccatgcgtg gctccttgtt gtgttgtttg caatttcttc atcataccgt ttattcaatg
     5761 tgctgtgagt aactttcact tccgtatttg cataacgatg ttttaacatc tgctgatgaa
     5821 aggcagcggc aattacaata attatcgctg tgaatactgg attatgtgcg ccgcctcacg
     5881 cacaataatc aagctgtaaa tcagcttaat aactttgccc ccacgcaggg tggaggcgtc
     5941 acacctgcag gagaaatcat aaatgccatc actcagtaaa gaagcggccc tggttcatga
     6001 agcgttagtt gcgagaggac tggaaacacc gctgcgcccg cccgtgcatg aaatggataa
     6061 cgaaacgcgc aaaagcctta ttgctggtca tatgaccgaa atcatgcagc tgctgaatct
     6121 cgacctggct gatgacagtt tgatggaaac gccgcatcgc atcgctaaaa tgtatgtcga
     6181 tgaaattttc tccggtctgg attacgccaa tttcccgaaa atcaccctca ttgaaaacaa
     6241 aatgaaggtc gatgaaatgg tcaccgtgcg cgatatcact ctgaccagta cctgtgaaca
     6301 ccattttgtt accatcgatg gcaaagcgac ggtggcttat atcccgaaag attcggtgat
     6361 cggtctgtca aaaattaacc gtatcgtgca gttctttgcc cagcgtccgc aggtgcagga
     6421 acgtctgacg cagcaaattc ttattgcgct acaaacgctg ctgggcacca ataacgtggc
     6481 tgtctcgatc gacgcggtgc attactgcgt gaaggcgcgt ggcatccgcg atgcaaccag
     6541 tgccacgaca acgacctctc ttggtggatt gttcaaatcc agtcagaata cgcgccacga
     6601 gtttctgcgc gctgtgcgcc atcacaactg attaaaaggc aggaaccatg gagcgcaacg
     6661 tcacgctcga ttttgttcgc ggcgtcgcca ttctggggat cctgctatta aacatcagcg
     6721 cctttgggct accaaaggcg gcttatctca atcccgcctg gtacgatgct attacgccgc
     6781 aggatgcatg gacctgggca tttctcgatc tcatcggcca ggtgaaattc ctcacgcttt
     6841 ttgcgctgct gtttggtgct ggcctgcaaa tgttgctgcc ccgtggcaga cgctggatcc
     6901 agtcgcggtt aacgctgtta gtcttgttgg gctttattca cggtttattg ttctgggacg
     6961 gcgatattct gctggcttac gggctggtgg ggcttggcgt tttgctgtta ttggggctga
     7021 tttccgacag ccagaccagc cgcgcctgga cgccggatgc atcggctatt ttgtatgaaa
     7081 aatactggaa gcttcacggc ggcgttgaag cgatcagtaa tcgtgccgat ggtgttggca
     7141 acagtttact ggcactgggc gcacagtatg gctggcaact ggcagggatg atgctcattg
     7201 gtgcggcgct gatgcgcagc ggctggctga aagggcagtt cagcttacgt cactatcgtc
     7261 gtactggttt tgtgctggtg gcgattgggg tgaccattaa ccttcctgcc atcgccctgc
     7321 aatggcagct ggactgggcg tatcgctggt gtgccttctt acttcaaatg ccgcgggaac
     7381 tgagtgcgcc gtttcaggcg attggctatg cgtcgctgtt ttatggcttc tggccgcaat
     7441 tgagccgctt taagctggtg cttgcgatcg cctgcgtcgg acggatggcg ctgaccaact
     7501 atctattgca aacgctgatt tgtaccacgc ttttttacca cctcggtttg tttatgcatt
     7561 ttgaccgcct ggagctgctg gcgtttgtta ttccggtatg gctggcgaat atcctcttct
     7621 ctgttatctg gctgcgttac ttccgccagg ggccggtgga atggctctgg cgtcagttaa
     7681 ctttgcgtgc tgccggaccg gcaatatcta aaacatcaag ataacgatct ggattacatt
     7741 cgttaacaaa acggctgtaa ccgtttccat tgctgtgact cgattcacga agtcctgtat
     7801 tcagtgctga caaaatagcc gccagcaagc agtcatttac tgcaatctca taacaggtag
     7861 tgaatatgat caccattcgt gatgtagcgc gtcaggctgg cgtctctgtg gcaacggttt
     7921 cccgggtgct caataacagc acgctggtca gtgccgacac gcgtgaagca gtaatgaaag
     7981 ccgtgagtga gctggattat cggccaaacg ccaatgccca ggcgctggca actcaggtta
     8041 gcgacaccat tggcgtggtg gtgatggacg tttctgatgc gtttttcggc gcgctggtaa
     8101 aagcggtgga tctggtcgcc cagcagcatc agaaatacgt gctaatcggc aatagctatc
     8161 atgaagcgga aaaagagcgt cacgccattg aggtgttaat tcgccagcgt tgtaatgcgt
     8221 tgattgttca ctcaaaagca ttgagtgacg atgaactggc gcaatttatg gataacattc
     8281 ccggtatggt gttaatcaac cgcgttgtgc cggggtacgc ccatcgttgc gtttgcctgg
     8341 ataatctcag cggtgcccga atggcgacgc gcatgttgct gaataacggt catcaacgta
     8401 ttggttatct ttcttccagc cacggcattg aagatgacgc catgcgtaaa gcaggctgga
     8461 tgagtgcgtt gaaagagcag gatattattc cgccggaaag ctggattggc actggtacgc
     8521 cggacatgcc gggcggtgag gcggcgatgg ttgaactgct ggggcgcaat ctacaactta
     8581 ccgctgtatt tgcttataac gacaatatgg ccgctggcgc actgacagca ttaaaagata
     8641 atggcattgc gattccgtta catctctcaa tcatcggttt cgatgatatt cccatcgccc
     8701 gttacaccga cccgcaatta acgaccgtgc gttatcccat tgcttcaatg gctaaattag
     8761 ccaccgaact ggccttgcag ggggcagcag gcaatattga tcctcgtgcc agccactgtt
     8821 ttatgccgac gttagtgcgt cgccattctg tcgcaacgcg ccagaatgcg gcggcgatca
     8881 ctaactcaac aaatcaggcg atgtaaccgc tttcaatctg tgagtgattt cacagtatct
     8941 taacaatgtg atagctatga ttgcaccgtt ttaatgttgt aacccgtatg taacagtgaa
     9001 taatcacttt caccgaggta atagcgtcat aacaacaatt aaagccgttt tctggagcgt
     9061 taccgggcat ggaagaacga attttaaaaa gtgagcttcg gcgttcagta acacttcatt
     9121 aactctactg cctcgccgag catttatctc aagcactacc ctgcataaga aaaaccggag
     9181 ataccatgaa taagaaggtg ttaaccctgt ctgctgtgat ggccagcatg ttattcggtg
     9241 ccgctgcaca cgctgctgat actcgcattg gtgtaacaat ctataagtac gacgataact
     9301 ttatgtctgt agtgcgcaag gctattgagc aagatgcgaa agccgcgcca gatgttcagc
     9361 tgctgatgaa tgattctcag aatgaccagt ccaagcagaa cgatcagatc gacgtattgc
     9421 tggcgaaagg ggtgaaggca ctggcaatca acctggttga cccggcagct gcgggtacgg
     9481 tgattgagaa agcgcgtggg caaaacgtgc cggtggtttt cttcaacaaa gaaccgtctc
     9541 gtaaggcgct ggatagctac gacaaagcct actacgttgg cactgactcc aaagagtccg
     9601 gcattattca gggcgatttg attgctaaac actgggcggc gaatcagggt tgggatctga
     9661 acaaagacgg tcagattcag ttcgtactgc tgaaaggtga accgggccat ccggatgcag
     9721 aagcacgtac cacttacgtg attaaagaat tgaacgataa aggcatcaaa actgaacagt
     9781 tacagttaga taccgcaatg tgggacaccg ctcaggcgaa agataagatg gacgcctggc
     9841 tgtctggccc gaacgccaac aaaatcgaag tggttatcgc caacaacgat gcgatggcaa
     9901 tgggcgcggt agaagcactg aaagcacaca acaagtccag cattccggtg tttggcgtcg
     9961 atgcgctgcc agaagcgctg gcgctggtga aatccggtgc actggcgggc acagtactga
    10021 acgatgctaa caaccaggcg aaagcgacct ttgatctggc gaaaaacctg gccgatggca
    10081 aaggtgcggc tgatggcacc aactggaaaa tcgacaacaa agtggtccgc gtaccttatg
    10141 ttggcgtaga taaagacaac ttggctgaat tcagcaagaa ataagactga ttgggcgcag
    10201 tcgtttactg cgcccttatt gtatggccaa caaggtaaaa ttatatggtc agctcaacga
    10261 ctccgtcttc cggggaatac ttgttggaaa tgagcggtat caacaagtcc tttcctggtg
    10321 ttaaggcact tgataacgtt aatttaaaag tccggccaca ttctatccat gcattaatgg
    10381 gggaaaacgg tgcaggaaaa tcgacattat taaaatgcct gtttggtatt tatcaaaaag
    10441 actccggcac cattttattc cagggtaaag agatcgattt ccattccgcc aaagaggcac
    10501 tggaaaatgg tatttcgatg gtacaccagg agttaaacct ggtattacaa cgttcggtga
    10561 tggataacat gtggctgggg cgatatccca ccaaaggcat gtttgtcgat caggacaaaa
    10621 tgtaccgcga aaccaaagcg atttttgatg aactggatat tgatatcgat ccgcgtgcgc
    10681 gcgtcggcac attatccgtt tcgcaaatgc agatgatcga aatcgccaaa gcgttttcct
    10741 ataacgcgaa aattgtgatt atggatgaac cgacttcttc gttaaccgaa aaagaggtca
    10801 atcatctgtt cactattatt cgtaaattaa aagagcgcgg ctgcggtatt gtttatatct
    10861 cgcataaaat ggaagaaatc ttccagttat gtgatgaagt taccgtattg cgcgacggtc
    10921 agtggatcgc caccgaaccg ctggcaggac tgacgatgga caagatcatc gccatgatgg
    10981 ttgggcgttc tcttaatcag cgttttcctg acaaagaaaa caagccgggc gaagtcatcc
    11041 tcgaggtgcg taacctaacg tcactgcgcc agccgtcgat tcgcgatgtc tcgtttgatc
    11101 tgcataaagg ggagatcctc ggtattgccg ggctggtggg ggcgaaacgt accgatattg
    11161 ttgagacgtt atttggtatt cgcgagaaat cggctggcac cattacgttg cacggcaaac
    11221 agatcaataa ccataatgcc aacgaagcca taaaccacgg atttgcactg gtaactgagg
    11281 agcgccgctc aacgggaatt tatgcctatc tggatattgg ttttaactcg ttaatttcca
    11341 atattcgcaa ctacaaaaat aaagttggtt tactggataa ctcgcggatg aaaagcgata
    11401 cccagtgggt gattgattcg atgcgggtaa aaacgccggg tcatcggacg caaattggtt
    11461 cgctctccgg tggtaatcag caaaaggtga ttattggtcg ctggctacta acgcaaccag
    11521 aaatattaat gctcgatgaa ccgacgcgcg gtattgatgt cggggcgaag tttgaaattt
    11581 atcagttaat tgccgaactg gcgaagaaag gcaaggggat tattattatc tcctctgaaa
    11641 tgcctgagtt gttagggata acagaccgta ttctggtcat gagcaatggt ctcgtttccg
    11701 gaattgtcga tacaaaaaca acaacgcaaa acgaaattct gcgtcttgcg tctttgcacc
    11761 tttaagatta ggggcttccc atgagtgcgt taaataagaa aagttttctt acttacctga
    11821 aagagggcgg tatttacgtc gttcttttag ttttgctggc gattattatt ttccaggacc
    11881 caacattttt aagtctgttg aacttaagta atattctcac ccagtcatcg gtgcgtatta
    11941 ttatcgcgct cggtgtggca gggttaattg tcacccaggg gaccgatctt tctgctggtc
    12001 gtcaggtagg gctggcggca gtggtggctg cgacattatt gcagtccatg gataacgcca
    12061 acaaagtgtt cccggaaatg gcgacgatgc cgattgcgct ggttattctg attgtctgtg
    12121 ccattggtgc ggtgatcggt ttgatcaacg gtctgattat cgcttatctc aacgtgacgc
    12181 cgttcattac cacgctcggc acgatgatca tcgtctatgg catcaactcg ctctattacg
    12241 actttgtcgg ggcgtcgcca atttctggtt ttgacagtgg cttctctacc tttgctcagg
    12301 gctttgtcgc gctggggagt ttccgtctct cttacatcac cttctacgcg ttgattgcgg
    12361 tggcgttcgt ctgggtgttg tggaacaaaa cccgcttcgg taagaacatt tttgccattg
    12421 gcggtaaccc ggaagcggca aaaatatctg gtgtcaacgt cggcctgaac ctgctgatga
    12481 tctacgcgtt gtctggcgtg ttctatgcct ttggcgggat gttagaagcc ggacgtatcg
    12541 gctctgccac caacaacctc ggctttatgt atgagctgga tgctatcgcg gcgtgcgtgg
    12601 taggcggtgt atcgttcagc ggcggtgtgg ggacggtgat tggcgtggtg accggggtaa
    12661 ttatttttac cgtcatcaac tatggcctga cgtatatcgg cgtaaaccca tactggcagt
    12721 acatcatcaa aggggcgatt attatcttcg ccgtagcgct ggattcactg aaatacgcgc
    12781 gtaagaagtg atgtgattgc ggatattgcc ggatgcgtct ctggttttac aacgttaccg
    12841 ggtgttcttt ctcgcctttc ttaaacttca cttccccgag ctcaatacaa cccaccgggc
    12901 agacgtgacc acaaagcaga caacccacac atttctcggt attacaatgc ggtgtgcggg
    12961 ttttctcgct ccattccatc gcctggtgac cgccgtcgta acaggaaata taacagcgtc
    13021 cacagccaac acatttgtca agattgatac ggggatagac aatataactg cggtctaaat
    13081 cttccgccgg gacaatattg ttattcgcca gacctaccat ctcctgcaac gaatcaaatc
    13141 cctgatcggc gagataatgc gataacccgc tcgccatatc ttctacgatg cgatagccgt
    13201 actgcataat gccggtggtg acctgtaacg ttgctgcgcc gagcaataaa aactccgccg
    13261 catcttccca ggtttcaatg ccgccgatac cgctgattgg gaaatcgcgc agttctggat
    13321 gggtgcgcat ttgctgaatg aagcgcaggg cgatcggttt taccgcttta ccggaatatc
    13381 cggaaatact cgatttaccg ttaacgatcg gcataccgat tttctgatta agatcgatat
    13441 tggtgatgga tttaacggtg ttaattgccg caatgccatc tgcgccgccg cgcttcgccg
    13501 ccagcgccac ttcgcacata tcaccgatat tcggcgtcat cttcgccagc attggcagcg
    13561 tggaaccccg tttcactgcc cgacaatatt tttctaccag ctccgggctt tgcccgacat
    13621 cgctacccat cgcatgagaa gtcatttgcg gacaggagaa gttacactcg atcatatccg
    13681 cgccagctgc tcccattgct gctcattttc ccccatgatc gaagcgatca atactttgtc
    13741 cgggtaatct tccttcagcc gacgcagggc ggtcagattc tcttccaacg gatgttcagc
    13801 aatctgctcc atatttttga agccgataaa accggtatct tctttcacca gatgatcaaa
    13861 acgcggcgag acttcgttgg cgataaaaaa gccgatcgtt ttaaacacca caccgcccca
    13921 acctgtgtcg taggctttgg cacacatctc atagcagttg cctaccggcg aagaagagag
    13981 gcagaacggg ttgggaaagt tcacgccgca aaaagtaatc gaaagatctt tcgttaacat
    14041 gagcaagctc cctctaaata gtgatgaatc gcctcggcgg cttctttccc ggttttcacg
    14101 gcatagacca cggttttgtc accctcaaca atatcgccag cagcaaagac ttgcggatcg
    14161 cggatctgat aattttgtgt tttgatggtg ttacgctgct gctctaactc cgcaaaggca
    14221 tccagtctgg catgctgacc gacggcgaga ataattttat ctgccgccat cgtcagttcg
    14281 cctgataacc gtacatgctt aaacgtgact ttattgcctt ccacggctac tggcgtgaat
    14341 ccatcgatga tcgaaacgcc cagctcccgg gcgctggcaa actctttttc gcttgccgga
    14401 aactcatcta actcttcacg tgctacgcaa gttaccgcct gacagccgag aactttcagc
    14461 gtgctggcta cgtccatcgc gacatcaccg ccgccgataa ttaatgcgct ttgtggaatg
    14521 ctgatatcgc cttgcgcctg tcgtacacgt tgcaggaagt cgacggcaat ctcaacgtcg
    14581 ctatgctcaa acagcggtag cccggaaccg cttgataacc ccacggtgac cagtaccgcg
    14641 cggttttccg ctttcagttg ctccagggtg agtgttttac cgatttcgtt attgcactta
    14701 atggtcacgc ccattttttc aatacgggcg atctctgcat ccagcactga ttgcggtaac
    14761 cggaattgcg gaataccgtt acgcaaccag ccaccggggt gcgcttcttt ctcataaatc
    14821 gtgacgtcat aaccctggtt tgtcagtgtc acactggcct gcaatccggc aggacctgcg
    14881 ccaataatcg cgactttgcc gagcgtttta gtaccgggct gataaatttc catcccggtt
    14941 tgttgttcaa aatcagtaac aaaacgctgt aagcggccga tatcaatggg agcatcaaca
    15001 ccggcacggg tacaaccgct ttggcataat ttctccgtcg ggcacactct ggcacaaacg
    15061 gcaccgaggg cattattttc gcgaattgtc tcggcagcgc ctttaaaatt acgaaagtag
    15121 attgagcgaa taaatttccc cggatcggtc tgtgccgggc aagcctgact acagggagcg
    15181 tcgtgacata ataaacagcg agaggcttct ttaatcgcca gtaacggcgt aaaagccgga
    15241 gtgagttcat ccagataatt ttgttgcggc atgtgatctt ccttaatgct atgagtaatt
    15301 atggcgaacg ctaaataaat attgcgtgct gatattaagg ctatgcgcgt ggggaaattt
    15361 aagctgtgaa aggattcaca aatagctgcg gttcagaatg ttatatccgc agcttattga
    15421 gtgaagtaat aatt
//
LOCUS       DAZSNM010000088        15408 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZSNM010000088 DAZSNM010000000
VERSION     DAZSNM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15408
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.088"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1173)
                     /locus_tag="ACHW42_002271"
     CDS             complement(<1..1173)
                     /locus_tag="ACHW42_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8944076.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1372..1456)
                     /locus_tag="ACHW42_002272"
     tRNA            complement(1372..1456)
                     /locus_tag="ACHW42_002272"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Leu,seq:caa)
     gene            1652..2671
                     /gene="ahr"
                     /locus_tag="ACHW42_002273"
     CDS             1652..2671
                     /gene="ahr"
                     /locus_tag="ACHW42_002273"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR8944077.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2800..4302
                     /locus_tag="ACHW42_002274"
     CDS             2800..4302
                     /locus_tag="ACHW42_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR8944078.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4421..5503)
                     /gene="lptG"
                     /locus_tag="ACHW42_002275"
     CDS             complement(4421..5503)
                     /gene="lptG"
                     /locus_tag="ACHW42_002275"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR8944079.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5503..6603)
                     /gene="lptF"
                     /locus_tag="ACHW42_002276"
     CDS             complement(5503..6603)
                     /gene="lptF"
                     /locus_tag="ACHW42_002276"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR8944080.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6666..6716)
                     /gene="ytgA"
                     /locus_tag="ACHW42_002277"
     CDS             complement(6666..6716)
                     /gene="ytgA"
                     /locus_tag="ACHW42_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR8944081.1"
                     /translation="MSIIILATISTTQENK"
     gene            6870..8381
                     /gene="pepA"
                     /locus_tag="ACHW42_002278"
     CDS             6870..8381
                     /gene="pepA"
                     /locus_tag="ACHW42_002278"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR8944082.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8515..8958
                     /gene="holC"
                     /locus_tag="ACHW42_002279"
     CDS             8515..8958
                     /gene="holC"
                     /locus_tag="ACHW42_002279"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR8944083.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8958..11813
                     /gene="valS"
                     /locus_tag="ACHW42_002280"
     CDS             8958..11813
                     /gene="valS"
                     /locus_tag="ACHW42_002280"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR8944084.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11867..13063)
                     /locus_tag="ACHW42_002281"
     CDS             complement(11867..13063)
                     /locus_tag="ACHW42_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR8944085.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13256..13759
                     /locus_tag="ACHW42_002282"
     CDS             13256..13759
                     /locus_tag="ACHW42_002282"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8944086.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13805..14221)
                     /gene="rraB"
                     /locus_tag="ACHW42_002283"
     CDS             complement(13805..14221)
                     /gene="rraB"
                     /locus_tag="ACHW42_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR8944087.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14383..15396
                     /gene="argF"
                     /locus_tag="ACHW42_002284"
     CDS             14383..15396
                     /gene="argF"
                     /locus_tag="ACHW42_002284"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR8944088.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3976 a         3932 c         3968 g         3532 t
ORIGIN      
        1 caacctacgc tccccaagat gttccgcctt atggatgtaa gctgctcgca cagagctacg
       61 ctcctggtga ctcatctgcc gctctaccgc atccctcgac cataatcccg actcaatcaa
      121 cgaactacaa gccatcgtcc tgaagccatg cccacaaacc tcgaccttcg tgtcataccc
      181 cattacgcgc aaagccttgt tcaccgtatt ctcactcatc ggcttacgtg gatcgtgatc
      241 gcccacaaaa atcagctctc gatttccgct catgcttttg atcttttcca aaatacttag
      301 ggcctgccgc gacaaaggta caaggtgtgg tgtccgcatc ttcgagccac gctgagaatg
      361 cttgactcct tccagttgct cacgctcacc tgggattgtc cacatagccg tttcaaaatc
      421 tatttctgac cagcgggcaa aacgcagctc gctcgaacgg atgaaaacca ataaagtgag
      481 ttcaaccgcc agtcgagtta acggtctgcc agaatagtga tctatgcgat gaagtaattc
      541 aggtatacgg ttcagttcaa gagctgcacg gtgctgtctt ttcgccgtag caaccgcacc
      601 tgcaatctct tgcgcggggt tgtagtcgat taagccgctc tgaacggcaa agcgcataat
      661 cgcggtagta cgctgctgta aacgggcggc aacttcgagc cgcccggatg actcgactgc
      721 tttaatgggt acaagaagat cccgcgtctt cagttccgca atgttccgct taccgatagc
      781 agtaaagaga ttatcctcca gacttttcaa aacacgagcg ctatgcgatg ccgaccactt
      841 ctgattgctg gcgtgccagt ctctggcgac tacttcaaac gttatcgcct cttgttcctg
      901 ttctacctta acagctttct tgttttcact gggatcgaca ccattagcta aaagcttacg
      961 agcctcatcc cggcgtgccc tggcatccgc caacgacact tcggggtatt tccccaatgc
     1021 cagcatcttc tctttgccgc caaagcgata acgtagccgc cagtatttgg agccgttagg
     1081 gtgaaccagc aaaaccatgc cttcaccgtc ggtcagctta taggcttttg cttcaggctt
     1141 agccgaacga accttcacat cactcagagc catgatgagt atcctttcaa gggttctgtg
     1201 tgggtacaaa cattatcgaa ccgggaaata cccgcagttg tacccgcatc agtaagttga
     1261 tgtagattga atcaggttga cttaggttga gtgaaaaagc gaggaaagcc ttgcggatac
     1321 tggattttag gcacaaaaaa agacgtccgt tgacgtctat tgatgttccg atggtgccga
     1381 aggccggact cgaaccggca cgtatttcta cggttgattt tgaatcaact gcgtctaccg
     1441 atttcgccac ttcggcactg aagaggtatg cggaaaacgt tgtggattat acctgtcgca
     1501 cgccaccatg caagcgccag cttgcaccca cctcgctaag tgctgaaatt ttcagcatta
     1561 cactcagcga tatgtaacct ttgtcacact ccaggcaccc cgccctgcca tgctctacac
     1621 ttcccaaaca acaccagaga aggccccaaa aatgtcgatg ataaaaagct atgccgcaaa
     1681 agaagcgggc ggcgaactgg aagtttatga gtacgatccc ggtgagctga agccacaaga
     1741 tgttgaagtg caggtggatt actgcgggat ttgtcattcc gatctgtcga tgatcgataa
     1801 cgaatgggga ttttcacaat atccgctggt tgccgggcat gaggtgattg ggcgcgtagt
     1861 ggcactcggg agcgccgcgc aggataaagg tttgcaggtc ggtcagcgtg tcgggattgg
     1921 ctggacggcg cgtagctgtg gtcactgcga cgcctgtatt agcggtaatc agatcaactg
     1981 cgagcaaggt gcggtgccga caattatgaa tcgcggtggc tttgccgaga agttgcgtgc
     2041 ggactggcaa tgggtgattc cactgccaga aaatattgat atcgagtccg ccgggccgct
     2101 gttgtgcggc ggtatcacgg tctttaaacc actgttgatg caccatatca ctgctaccag
     2161 ccgcgttggg gtaattggta ttggcgggct ggggcatatc gctataaaac ttctgcacgc
     2221 aatgggatgc gaggtgacgg cctttagttc taatccggcg aaagagcagg aagtactggc
     2281 gatgggtgcc gataaagtgg tgaatagccg cgatccgcag gcactgaaag ccctgtcggg
     2341 gcagtttgat ctcattatca atactgtgaa cgtcagcctc gactggcagc cttattttga
     2401 ggcgctgacc tatggcggta atttccatac ggtcggtgcg gttctcacgc cgctgtctgt
     2461 tccggccttt acgttaattg cgggcgaccg cagcatctct ggttctgcta ccggcacgcc
     2521 ttatgagctg cgaaagctga tgcgctttgc cgcccgcagc aaggttgcgc cgacaaccga
     2581 actgttcccg atgtcgaaaa ttaacgacgc cattcagcat gtacgcgatg gtaaagcgcg
     2641 ctaccgagta gtcctgaaag ccgacttctg atccctttgc cgggtgctgt cgcccggcac
     2701 ttaaatctct ttggaaggcg tctcgcaatc tgctgaattt agcggtgaag agtgcacagc
     2761 cgttgcctat actccaggca ggaaactgga ggaaatctca tgagcgaacc cctgttaatt
     2821 gcccgcacgc cggacacaga actgttttta ctgccgggaa tggctaaccg tcacgggctg
     2881 attactggcg caacggggac gggtaaaacc gttacgctgc aaaaactggc agagtcattg
     2941 tcggaaatcg gcgtgccggt gtttatggct gatgtgaaag gcgatctgac cggtatcgcg
     3001 caggcaggaa cggcgtcgga aaaactgctc acaaggctta aaaatatcgg cgtcaatgac
     3061 tggcaaccgc atgccaatcc ggtggtggtg tgggatatct ttggcgagaa aggccacacg
     3121 gtgcgggcga cggtttcgga tctggggccg ctgttgctgg cgcgactgtt gaatctcaac
     3181 gatgtgcaat ctggcgtgct gaatattatc ttccgcattg cggacgatca gggattgttg
     3241 ctgctcgact ttaaagattt gcgggcgatt acccagtaca tcggcgataa cgccaaatcc
     3301 ttccagaatc agtacggaaa tatcagtagc gcatcggttg gtgccatcca gcgcggatta
     3361 ctgtcgctgg aacagcaagg cgcagcacac ttctttggtg agccgatgct ggatatcaaa
     3421 gactggatgc gcaccgatgc caacggtaaa ggcgttatca atatcctcag cgccgagaaa
     3481 ctttatcaga tgccgaaact gtacgccgcc agcctgctgt ggatgctttc agagttgtat
     3541 gaacaattgc cggaagcggg cgatctggag aaaccaaaac tggtgttttt cttcgacgag
     3601 gcacatctgc tgtttaacga tgcaccgcag gtactgctgg ataagattga gcaggtgata
     3661 aggcttattc gctcaaaagg cgtgggcgtc tggttcgttt cgcaaaaccc gtctgatatt
     3721 ccggataatg tgttcggcca gctcggtaat cgtgttcagc acgctttgcg ggcttttacg
     3781 ccaaaagatc agaaagcagt gaagacagcg gcgcaaacca tgcgggccaa tccggcattt
     3841 gataccgaaa aggcaatcca ggaactgggg accggcgagg cgttaatctc gtttctcgat
     3901 gcaaaaggaa gtccttctgt ggtggaacgg gcgatggtga tcgcaccttg ttcgcgaatg
     3961 gggccggtga cggaagatga gcgtaatggc ctgattaatc actccccggt gtatggcaaa
     4021 tatgaagatg aggtggaccg cgagtccgcc tatgagatgc tgcaaaaagg ctttcaggcc
     4081 agtaccgagc agcaaaataa tccccccgtg aaagggaaag aggtggcggt ggatgacggt
     4141 attcttggtg gattgaagga tattttgttt ggcactaccg gaccacgcgg cgggaagaaa
     4201 gatggtgtgg tgcaaacaat ggcgaaaagc gccgctcgcc aggtgacgaa tcagattgta
     4261 cgcgggatgt tggggagttt gctggggggg agaaaaagat aagtcagacg tgatctgtgc
     4321 ccctcaccct aaccctctcc ccaaaggggc gaggggaccg ttcgaactcg tatttgactt
     4381 tgtcaacaat aataaaggga gctttcgctc ccttgttaga ttacgatttt ctcattaaca
     4441 gccacaggct gattaagaag aagctggcac ttggcaacag tgcgccgatg atcggcggga
     4501 tgccgtaaac caacgtcagc gggccgaaga tctggtccag tacgtagaag acaaaaccga
     4561 agctgatacc tgtgaccaca cgcacgccca tcggtacgct acgcagtggg ccaaagataa
     4621 acgacagcgc catcagcatc atcaccgcca cggatagcgg ctggaagatt ttgctccaca
     4681 tgttgagctg ataacgtccg gcatcctgac cgctcgactt cagatacttc acgtagttgt
     4741 gcaaaccact gatggagagt gcatccgggt ccagcgccac cacgcccagt ttgtctggcg
     4801 tgaggtcggt tttccaggtg ccgctcaccg tctgcgaacc ggtaatctgt ttcggattgg
     4861 tcagatcaga ttcatcaacc tgcgacagac gccagacttt atgttccggg tcaaacttcg
     4921 cggtagcggc ataacgtacg gattgcagac gacgattctc gttaaaggca taaatactga
     4981 tgccacctaa cacctcgtca cctttaaccc gctcaatgta gacgaagttg ttgccatctt
     5041 tcgcccataa accttgctgg gtagagagca acgagccgcc gtacatcgcc tgcgcacggt
     5101 agttacgcgc catctgttcg ccctgcggcg cgacccattc gccaatcgcc atcgtcagca
     5161 agaccagtgg aatggcggtt ttcatcaccg acagcgctac ctgcatacgg gtaaaaccag
     5221 aagcctgcat caccaccagt tcgctgcgct gcgccagcat cccaagacca agcaacgccc
     5281 caagaagagc cgccatcggg aagaagatct gcacatcttt cggcacgctc agcaaggtat
     5341 acattcctgc gcctaacgcg tcgtaactcc cctgcccggc ttttttcagc tgatcgacaa
     5401 atttgataat gcccgacagc gacaccagca tgaacagcgt catcatgatg gtggtgaaaa
     5461 tagttttacc gatatagcgg tcaagtacgc caaaaggttg catcacaccg ctcctttacg
     5521 cgaaaaactg gcgcgcaggc ggcggaccgg cacggtgtcc caaaggttga gaacaatcgc
     5581 caaagccaga taaatcaggt taacggtcca catccacagt gtcgggtcca gcttaccttt
     5641 accgccgttc gatttcaggg aggtctggat caggaagaaa agtagataca gcagcatggc
     5701 tggcagcatc gacagtacgc gtccctgacg cgggttaacc acgctcaacg gtacaaccat
     5761 aagtgccatc ataaacacgg tgaataccaa cgtgatacgc cagttcagtt ctgcgcgagc
     5821 acgatcggtg tcagtgttcc acaatgtgcg catgtccatc tggtcggtat cgttcgggtc
     5881 gagcgccacc gcctggtgac caatgatcgc ctgataatcc tggaaatccg taatgcggaa
     5941 atcacgtaac agtgcagtgc cttcgaagcg cgttccctgg ttgagagtga cgacctggga
     6001 gccgtcgcgc agctgggtta aatgtccgga atcggccacc accacagaag gacgtgcatt
     6061 accttttggt cgaatttgtg cgaggaacac atctttgaaa tcgctgccgt caacgctttc
     6121 gatgaacagc accgagctgc cattagtcgc ttgctggaat tgcccttgcg ccagcgccgc
     6181 catgccaggg ttcgctttcg cttctgctaa cacttcatcc tgatgacgcg atgaccacgg
     6241 tcccgcccac atcacgttaa ccgccgcgac gattgccgtg aataccgcaa ggatcattgc
     6301 cgctttcacc agaaccgctt tgctcaagcc gcaggcatgc attaccgtaa tttcactttc
     6361 ggtatacagt ttgcccagcg tcatcagcag cccgaggaac aggcttaatg gcaggataag
     6421 ctgcgccatt tccggcacgc ccaacccgag aagggagagc accagattcg ccggaatatc
     6481 gccgtcaacc gctgcgccga ggatcctcac taacttttga cagaagaaga tcaaaagcaa
     6541 gatgaagagt atcgccagct ggcttttgag cgtctcccgc accagatatc ttatgattat
     6601 cactttaaat acgcccgtaa aaactcgtct tttgcaggat tttagcttgt ttcatggctt
     6661 aaacgtcatt tattctcttg agtcgtcgaa atcgtcgcta agataattat actcaacgga
     6721 ttcacctctc agattttgtt ctgacgtgcc aatgccgtaa taacgttaag attaacacga
     6781 agtcatcgca acagcggaca tgagttacga aagcttgcaa ttctatctgt agccaccgcc
     6841 gttgtcttta agattcagga gcgtagtgca tggagtttag tgtaaaaagc ggtagcccgg
     6901 agaaacagcg gagtgcctgc atcgtcgtgg gcgtcttcga accacgtcgc ctttctccga
     6961 ttgcagaaca gctcgataaa atcagcgatg ggtacatcag cgccctgcta cgccggggcg
     7021 aactggaagg aaaaccgggg cagacattgt tgctgcacca tgttccgaat gtactttccg
     7081 agcgaattct ccttattggt tgcggcaaag aacgtgagct ggatgagcgt cagtacaagc
     7141 aggttattca gaaaaccatt aatacgctga atgatactgg ctcgatggaa gcggtctgct
     7201 ttctgactga actgcacgtt aaaggccgta acaactactg gaaagtgcgt caggctgtcg
     7261 agacagcaaa agagacgctc tacagtttcg atcagttgaa aacgaataag agcgaaccgc
     7321 gtcgtccgct gcgtaaaatg gtgttcaacg tgccgacccg ccgtgaactg accagcggtg
     7381 agcgcgcgat ccagcacggt caggcgattg ccgccgggat aaaagcagcg aaagatctcg
     7441 gtaatatgcc gccgaatatc tgtaacgccg cttatctcgc ctctcaggcg cgccagctgg
     7501 ctgacagcta cagcaagaat gtcatcaccc gcgttatcgg cgaacagcag atgaaagagc
     7561 tgggaatgca ctcttatctg gcggtcggtc agggttcaca aaacgaatcg ctgatgtcgg
     7621 tgattgagta caaaggcaac gcgtcggaag atgcacgccc aatcgtgctg gtgggtaaag
     7681 gtttaacctt cgactccggc ggtatctcca tcaagccttc agaaggcatg gatgagatga
     7741 agtacgatat gtgcggcgcg gcggcagttt acggcgtgat gcgtatggtc gcggagctgc
     7801 aactgccgat taacgttatt ggcgtgctgg caggctgtga aaacatgcct ggcgggcgtg
     7861 cctatcgtcc gggcgatgtg ttaaccacca tgtccggtca aaccgttgaa gtgctgaaca
     7921 ccgatgccga aggccgcctg gtactgtgcg acgtgttaac ttacgttgaa cgttttgagc
     7981 cggaagcagt gattgacgtg gcgacgctga ccggtgcctg cgtgatcgcg ctgggtcatc
     8041 atattactgg tctgatgacg aaccataatc cgctggccca tgaactgatt gccgcgtctg
     8101 aacaatccgg tgaccgcgca tggcgcttac cgctgggtga cgagtatcag gaacagctgg
     8161 agtccaattt tgccgatatg gcgaacattg gcggtcgtcc tggtggggcg attaccgcag
     8221 gttgcttcct gtcacgcttt acccgtaagt ataactgggc gcacctggat attgcaggaa
     8281 ccgcctggcg ttctggtaaa gcaaaaggcg caaccggtcg tccggtagcg ttgctggcac
     8341 agttcctgtt aaaccgcgct gggtttaacg gcgaagagta agtttgcgtt ttaccctcac
     8401 cctaaccctc tccctaaggg agagggaacc gatcgagcgc aatagaaatt cattgctcat
     8461 ctttcagggt gaggaatgca gcaatattta aatccaccac aagaagcccc atatatgaaa
     8521 aacgcaacgt tctaccttct ggacaatgac accaccgtcg atggcttaag cgccgttgag
     8581 caactggtgt gtgaaattgc cgcagaacgt tggcgcagcg gtaagcgcgt actcatcgcc
     8641 tgtgaagatg aaaagcaggc ttaccggctg gatgaagccc tgtgggcgcg tccggcagaa
     8701 agctttgttc cgcataattt agcgggagaa ggaccgcgcg gcagtgcgcc ggtggagatc
     8761 gcctggccgc aaaaacgcag cagcagcccg cgcgatatat tgattagtct gcgaacaagc
     8821 tttgcagatt ttgccaccgc tttcacagaa gtggtagact tcgttcctta tgaagattct
     8881 ctgaaacaac tggcgcgcga acgctataaa gcctaccgcg tggctggttt caacctgaat
     8941 acggcaacct ggaaataatg gaaaagacat acaacccaca agatatcgaa cagccgcttt
     9001 acgagcactg ggaaaagcag ggctacttta agcctaatgg cgatgaaagc caggaaagtt
     9061 tctgcatcat gatcccgccg ccgaacgtca ccggcagttt gcatatgggt cacgccttcc
     9121 agcaaaccat catggatacc atgatccgct atcagcgcat gcagggcaaa aacaccctgt
     9181 ggcaggtcgg tactgaccac gccgggatcg ctacccagat ggtcgttgag cgcaagattg
     9241 ccgcagaaga aggtaaaacc cgtcacgact acggccgcga agctttcatc gacaaaatct
     9301 gggaatggaa agcggaatct ggcggcacca ttacccgtca gatgcgccgt ctcggcaact
     9361 ccgtcgactg ggagcgtgaa cgcttcacca tggacgaagg cctgtccaat gcggtgaaag
     9421 aagttttcgt tcgtctgtat aaagaagacc tgatttaccg tggcaagcgc ctggtaaact
     9481 gggacccgaa actgcgtacc gctatttctg acctggaagt ggaaaaccgc gaatcgaaag
     9541 gttcgatgtg gcacatccgc tatccgctgg ctgacggcgc gaaaaccgca gacggtaaag
     9601 attatctggt ggttgcgacc actcgcccgg aaaccctgct gggcgatacc ggtgtagctg
     9661 ttaacccgga agatccgcgt tacaaagatc tgattggcaa atatgtcatt ctgccgctgg
     9721 ttaaccgtcg tattccgatc gttggcgacg aacacgccga catggaaaaa ggcaccggct
     9781 gcgtgaagat cacaccggcg cacgacttta acgactatga agtcggtaaa cgtcacgccc
     9841 tgccgatgat caacatcctg acctttgacg gtgatatccg tgaaagcgcc caggtgttcg
     9901 ataccaaagg taacgaatct gacgtttatt ccagcgagat cccggcagag ttccagaaac
     9961 tcgagcgttt tgctgcacgt aaagcagtcg ttgctgcggt tgatgcactc ggcctgctgg
    10021 aagaaattaa accgcacgac ctgaccgttc cttacggcga ccgtggcggc gtggttatcg
    10081 aaccgatgct gaccgaccag tggtacgtgc gtgccgatgt actggcgaaa ccggcagttg
    10141 aagcggttga gaacggcgac attcagttcg taccgaagca gtacgaaaac atgtacttct
    10201 cctggatgcg cgatattcag gactggtgta tctctcgtca gctgtggtgg ggtcaccgta
    10261 tcccggcatg gtatgacgaa gcgggtaatg tttatgttgg ccgcaacgaa gaagaagtgc
    10321 gcaaggaaaa taacctcggt gccaacgttg ccctgcgtca ggacgaagac gttctcgaca
    10381 cctggttctc ttctgcactg tggaccttct ctacccttgg ctggccggaa aataccgacg
    10441 ccctgcgtca gttccaccca accagcgtga tggtatctgg tttcgacatc atcttcttct
    10501 ggattgcccg catgatcatg atgaccatgc acttcatcaa agatgaaaat ggcaaaccgc
    10561 aggtgccgtt ccacactgtt tacatgaccg gtctgattcg tgacgacgaa ggtcagaaga
    10621 tgtccaaatc caagggtaac gttattgacc cgctggatat ggttgacggt atttcgctgc
    10681 cagaactgct ggaaaaacgc accggcaata tgatgcagcc gcagctggcg gacaaaatcc
    10741 gtaagcgcac cgagaaacag ttcccgaacg gcattgagcc gcacggcacc gatgccctgc
    10801 gcttcaccct ggcggcgctg gcgtctaccg gtcgtgacat caactgggat atgaagcgtc
    10861 tggaaggcta ccgtaacttc tgtaacaagc tgtggaacgc cagccgcttt gtgctgatga
    10921 acacagaagg tcaggattgc ggcttcaacg gcggcgaaat gacgctatcg ctggcggacc
    10981 gctggattct ggcggagttc aaccagacta tcaaagcgta ccgcgaagcg ctggacagct
    11041 tccgcttcga tatcgccgca ggcattttgt atgagttcac ctggaaccag ttctgcgact
    11101 ggtatctgga gctgaccaag ccggtaatga acggtggaac agaagccgaa ctgcgcggta
    11161 ctcgccatac gctggtgact gtactggaag gtctgctgcg cctcgcgcat ccgatcattc
    11221 cgttcatcac cgaaaccatc tggcagcgtg tgaaagtact ttgcggtatc actgccgaca
    11281 ccatcatgct gcagccgttc ccgcagtatg atgcatctca ggttgatgaa gccgcactgg
    11341 ccgacaccga gtggctgaaa caggcgatcg ttgctgtacg taacatccgt gcagaaatga
    11401 acatcgcgcc gggcaaaccg ctggagctgc tgctgcgtgg ttgcagcgcg gatgcagaac
    11461 gtcgcgtaaa tgaaaaccgt ggcttcctgc aaacgctggc gcgtctggaa agcatcaccg
    11521 tgctgcctgc cgatgacaaa ggtccggttt ccgttaccaa gatcatcgac ggcgcagagc
    11581 tgctgatccc gatggctggc ctcatcaaca aagaagatga gctggcgcgc ctggcgaaag
    11641 aagtggcgaa gatcgaaggt gaaatcagcc gcatcgagaa caagctggca aatgaaggct
    11701 ttgtcgcccg cgcaccggaa gcggttatcg cgaaagagcg tgagaagctg gaaggttatg
    11761 cagaagcgaa agccaagctg attgaacagc aggctgttat cgccgcactg taatcaacac
    11821 agtgatgaaa acgaaggccg gagcatgctc cggccttttt tatctcttac agaaatggta
    11881 aagaaggcat aactccccga gaaatactgg ctaagaagcc tttatctggt tgttcttctt
    11941 tattgattaa aggaaggtca tccaaatcgc ccagcaaata cgtatttttt gcctgccagt
    12001 caatcatcca tattttcagc agtgggtaag ccagaccacc cgtaatcccg gatatcacca
    12061 ccaacgcaca catgcgataa agcataccgt ggtacgttaa agttgagcga aaacgaatac
    12121 gcccatcatt cagtgacagg ttgctcataa aatgatttcg caacgacacc gttaaataac
    12181 tggtgctaac agcaatccca aaataataaa ttaactgcgc gattatcatt ttacgttgca
    12241 tttccataaa ttgctgtaaa ttctcaatat catcatttgc atacccagaa ctatcatact
    12301 catttaatat ttgatcgaag ataatataac cagcaacagc gagaaaaggt aataaagcta
    12361 aaaatgcgag gatggcatat tttatacaat acgcagtatc taatttcacc ttgaaacgat
    12421 gtataccgaa actggtattg ctccagagaa aactcattac caggctatat aaagtaccat
    12481 taaaaatacc aatggaaaca ataccaacta ttgccatcag aaccacagat ataataacac
    12541 tacttgaact attggcatgt agcatctttg tcgagataaa gaaaacagtc cccatcccaa
    12601 tggccattaa aatcggcaag aaaaaggtta cccaccagaa ccctttcaaa gagcaattaa
    12661 aactaaatct tacgccgttg agactggtca ttaaggcctg ataacgcagt ccttttgctg
    12721 ccataaatat aagcaaaacc aacagtaaca aagtcaaaac acagccaaca agcggcatat
    12781 ctgctgacac tgtcattaag attgcgaaat agaaaaaaac aaaaacaaga caactaacaa
    12841 aaacattccc accggtaatt ccataagaaa atcgttgtcc gttaacttcc atattagcat
    12901 aaagataacg cttacatttc attaatgccc atggtaaata gatacctagc gttataatcg
    12961 ttaacaacac attcaccaca caaataagaa aatatctctc acctgtacca tgaaaaacaa
    13021 aagaatggga cggaacatcc atttcattaa taacttgagc cataaaaatc ccctgaatta
    13081 aaaaatccat tgatataatt tatagataca gacagtaaaa tgttctgcca cgttacaata
    13141 ctcttatcag taataggctg gcaaaaactt tattgcttta taagacgctt gcgcacgtaa
    13201 gaagagagtg gtattaaaca aatgtataat tactttattt ttattgggtt aaactatgaa
    13261 taacattgcg ccgcaatcac ctgtaatgcg tcgcctgacg ctgcaggata atcctgctat
    13321 cgccagcgtc atccgtcagg tttacgcaga gtatggtctt accgccgata aaggctacac
    13381 cgttgccgat ccgaatctcg acgaactgta tcaagtgtac agccagcctg gccatgctta
    13441 ttgggtggtt gaatacaatg gcgaagtggt cggcggcggc gggattgcgc cattaacagg
    13501 tagtgaagcg gacatttgcg aactgcagaa aatgtacttt cttcccgcta ttcgcggtaa
    13561 agggctagcg aaaaaactgg ccttaatggc gatggagcag gcacgagaga tgggcttcaa
    13621 acgctgctat ctggaaacga ccgctttttt aaaggaagcc attgcgcttt atgagcattt
    13681 gggctttgag catatcgact atgcgcttgg ctgcacgggc catgtcgatt gtgaagtgcg
    13741 gatgctacgc gaactctaaa aaaatgcccg ctagtatgtg ctatcgggca ttttaatcaa
    13801 ttaattagtg gcgaaccccg tcatcgtctt catcgacaaa atcttcatcg tcgccatctt
    13861 cgccgttagg atcttcaaag taagtgcccc aaccgtcgta ctcaacgtca aatttctccg
    13921 ccagcgtcat cagttgttca acctgggcat cgatcagatc ggcattcaac gcgcactcgc
    13981 tgaggatgtc gcagcaaatc acgatatcac catcttcaac ttccagctct tctggatcgg
    14041 tcacttcgta accgagttta aacgcttcaa ctgccgcttt ttccagagtt tccagatcgt
    14101 ctgcggaaag atggtgttcg atggtgtaca gcgcgtccgg atcgctgcca tcttccagta
    14161 attcttcaat aatcaaacgt gtttcttcac gctgttcttc cagttgttcc gggtttgcca
    14221 tggctcattc ctcttaatgt cctgccgata ctcttattgt cacacaccac tgacattgcc
    14281 tccacctttg tgacaaagat ttatgcttta gacttgcaaa tgaataataa tacacataaa
    14341 ttgagtttta attcattgag gcgttagcca caggagggat ctatgtccgg gttttatcat
    14401 aagcatttcc tgaaattact cgatttcacg ccagctgaac tcaacagcct gctgcagtta
    14461 gccgcgaagc tgaaagctga taagaaaagc ggtaaagaag aagccaaact cactggtaaa
    14521 aacatcgcgc tcatcttcga aaaagactcg actcgtaccc gatgctcttt cgaagttgcc
    14581 gcatatgacc agggggctcg cgttacttat cttggcccaa gcggtagtca gattggtcat
    14641 aaagagtcga ttaaagacac tgcccgcgtg cttggtcgca tgtatgacgg tattcagtat
    14701 cgcggctatg gtcaggagat tgtcgaaaca ctggcggaat acgctggcgt gccggtatgg
    14761 aacggcctga ccaatgagtt tcaccccacg cagttgctgg cggatctcct caccatgcag
    14821 gagcatttac ccggtaaagc attcaacgaa atgacgctgg tctatgcagg tgacgcacgt
    14881 aacaacatgg gcaattcgat gctcgaagct gcggcgctta ccggtctgga tttgcgtctg
    14941 gtcgcgccac aggcatgctg gccagaagct gcgctggtta cggaatgccg cgccctggca
    15001 caacaaaatg gcgggaatat tacgctgacg gaagatgtag ctaaaggagt tgaaggtgct
    15061 gactttatct ataccgatgt atgggtgtcg atgggtgaag caaaagaaaa atgggctgaa
    15121 cgcattgcat tactgcgtga ttatcaggtg aacagcaaga tgatgcagtt gaccggtaac
    15181 ccggaggtca aattcctcca ttgcctgccc gcgtttcatg acgaccaaac gacgcttggc
    15241 aaaaaaatgg cggaagagtt tggcctacat ggcggaatgg aagtgactga tgaggtcttc
    15301 gaatctgccg ccagcattgt atttgatcag gcggaaaacc gcatgcatac catcaaagcg
    15361 gtgatggtcg cgacgctcag taaattaaat aattaatatc tctataaa
//
LOCUS       DAZSNM010000089        15249 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZSNM010000089 DAZSNM010000000
VERSION     DAZSNM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15249
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.089"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..218)
                     /locus_tag="ACHW42_002285"
     CDS             complement(<1..218)
                     /locus_tag="ACHW42_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR8944089.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIV"
     gene            complement(221..940)
                     /gene="sanA"
                     /locus_tag="ACHW42_002286"
     CDS             complement(221..940)
                     /gene="sanA"
                     /locus_tag="ACHW42_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR8944090.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1090..1974)
                     /gene="cdd"
                     /locus_tag="ACHW42_002287"
     CDS             complement(1090..1974)
                     /gene="cdd"
                     /locus_tag="ACHW42_002287"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR8944091.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(2104..2799)
                     /locus_tag="ACHW42_002288"
     CDS             complement(2104..2799)
                     /locus_tag="ACHW42_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR8944092.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(2796..3194)
                     /locus_tag="ACHW42_002289"
     CDS             complement(2796..3194)
                     /locus_tag="ACHW42_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR8944093.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            3433..4380
                     /gene="dusC"
                     /locus_tag="ACHW42_002290"
     CDS             3433..4380
                     /gene="dusC"
                     /locus_tag="ACHW42_002290"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR8944094.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(4751..4834)
                     /gene="yohP"
                     /locus_tag="ACHW42_002291"
     CDS             complement(4751..4834)
                     /gene="yohP"
                     /locus_tag="ACHW42_002291"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR8944095.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            5058..6494
                     /gene="mdtQ"
                     /locus_tag="ACHW42_002292"
                     /pseudo
     CDS             5058..6494
                     /gene="mdtQ"
                     /locus_tag="ACHW42_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            6586..7308
                     /locus_tag="ACHW42_002293"
                     /pseudo
     CDS             6586..7308
                     /locus_tag="ACHW42_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(7425..8003)
                     /locus_tag="ACHW42_002294"
     CDS             complement(7425..8003)
                     /locus_tag="ACHW42_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR8944096.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            8173..8760
                     /locus_tag="ACHW42_002295"
     CDS             8173..8760
                     /locus_tag="ACHW42_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR8944097.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            8934..9866
                     /gene="pbpG"
                     /locus_tag="ACHW42_002296"
     CDS             8934..9866
                     /gene="pbpG"
                     /locus_tag="ACHW42_002296"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR8944098.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9904..11619)
                     /gene="dld"
                     /locus_tag="ACHW42_002297"
     CDS             complement(9904..11619)
                     /gene="dld"
                     /locus_tag="ACHW42_002297"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR8944099.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            11815..14112
                     /gene="bglX"
                     /locus_tag="ACHW42_002298"
     CDS             11815..14112
                     /gene="bglX"
                     /locus_tag="ACHW42_002298"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR8944100.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            14364..>15249
                     /gene="osmF"
                     /locus_tag="ACHW42_002299"
     CDS             14364..>15249
                     /gene="osmF"
                     /locus_tag="ACHW42_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR8944101.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAA"
BASE COUNT         3802 a         3865 c         4088 g         3494 t
ORIGIN      
        1 acaatcatgc cgatagataa aattccgaag ccaatatatg ccaccatata agcgaagaag
       61 agtccgggat tttttgctcg ccagacatac accggttccg gtgcattttt aacccgttta
      121 tcaatcgcgc ctgcgcgcca gcctttccat gcttcgcgga aacaaaatat cgggataagg
      181 aaaaaaacgg caacgacaaa aaactgctgt acatccataa ctactttcct tgtttctttt
      241 gtaattcaag taactgttcg ggtgtaacgg cgggataccc ctgcgcatct tccggcacct
      301 ggtgcatagc cggaataggg accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat
      361 aaaggtcagc cagcgcaccg aaacgggcgg caaattcgcg aatacgtact gacagcatat
      421 ctttcggtga cggtacggca taacactgag cctgaatccc catatgcagc gcaataaata
      481 atgctcgctc acagtggaaa cgttgggtga taataatgaa atcattagta tcgaaaactt
      541 tgcgtgtacg cacgatggaa tccagcgtac gaaagcctgc gtaatcgaga acaatatctg
      601 atgggtcgac accagcagcg attaaatctt tgcgcatggt catcggctcg ttataacttt
      661 gcaatgcgtt atcgccgctc agtaatagat aatttacctt accgctgtta taggcattaa
      721 tcgctccttg aatgcggtag cgataatact gattaataac gccggtacga taatattttg
      781 ctgtgccgag caccacgccg acctggcggt aggggagatc ctgcaattcg tcgtagatat
      841 aaggcgcggt tttccagctc atccagcgat cgaggccgag cacagtcaac agcagcaagc
      901 cgatcaggac taacaggctg aggaacacgc gctttaacat gcaggagact cgagctaagg
      961 aaaatttcca tcaggctact ttacccgctg gttttgcgca agaaacggca aactgtttgc
     1021 cgggaatttc gacaccagga gagggctggc aagccggaca aggcgttcac gccgcatccg
     1081 gcaccaggct taagcgagaa gcacgcggtc gatactgtga cagccgagag ctttcagcgt
     1141 tgcggaggtg gcatcccact gaatcaacgg cgcatcggct ttttctgcca gaaccgcgcg
     1201 ctggatatcc gggtaatcat aacccttgag attcaacaga attaacgccc cctgcaacgg
     1261 cggcagagtc gggttgaatg cggcgttttc agcgtagctg ccgctgaaaa tacgaccgtc
     1321 tttacattcc agcgcgacac cgcttggtga cttactgtaa ggcatgtgcg aacggtttgc
     1381 cgctgcaatc gccgcctgag aaagcgcatc accagtcagc gcatagccgt gatcctgctc
     1441 gtccatcagc agcgttttaa tctccagatc tttcggccca aaggcatccg gcagatagtc
     1501 acgcagcgcg tgtgcctcgc ggcccggcaa atgaatacgc agatccagac cgctgttcag
     1561 ttcattcata aactgacggc agtgaccaca aggcgtatag ttaacggtga tggctgcaag
     1621 cgctttttca ccactcaacc aagcgtggct gatcgcgctt tgttcggcat gaacggtttg
     1681 ctgcattgtt gcaccaataa attccatatt ggcaccaaaa taccaggttc cgctcacacc
     1741 gcgcgcaatt gcgccaacat taaaattcga caatggcgta cgcgcacagg ccgccgccag
     1801 cggaagtagt gcgaatgcca gcgcgtcttc gtccagtccc gttgcgctct tcagcgatga
     1861 gacttgctcc ccggtcaaca aagcggggaa gtacttgtct gccagaatag gttccagtgc
     1921 agattgcaag ttatccgcaa gttgggcaaa agcggtttga aaacgtggat gcatggcgtt
     1981 gcctcataat catgtaatgg gataacagtt tacgaacccg ttatggcttt atatgtgatc
     2041 tgaatctcat taattatgca acacgtgaaa cttacatcaa aaatgcgcga cgtatcgcaa
     2101 attttagccc attactgcca gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat
     2161 tatcccgcat aacaccagcg ctagcgaact aaatgcacct tcctgataat ccagctcggc
     2221 gcagcgcgcc gtaccgaggg cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt
     2281 ggtacgaata cgcatcgcat taagcaatgt atggccaaat accgcgccga ggatgccgac
     2341 gaaaatcacg caaactgcgc tgattgccgg aataccgcca atactgccgc caaccgccat
     2401 tgcaataggc gtggtgactg atttcggcag gattgacgcg gcgatttccg gtgaagcgcc
     2461 catcaacaat gccacggaag tccccgtcac catcgcaacc acgctgccga taaaacagat
     2521 ggtgatgatc gatttccagc gcgcgcggat ctggtgtagc tgctcatata aaggataggc
     2581 cagcgcgacg accgccggtt gcagcagatc gtttagcact tcgctacctt taaagtaact
     2641 gtcgtaagag atgccggtca gcattaaaaa aggaatgatc accaccattg ctaccagcaa
     2701 cgggttaagc aggggaaact tataccgtgc cgccagtttg cgggcggcaa aaaagacaat
     2761 caacgtcagc ggtaatgacc accagatatt cgccatcatt cttctgatcc tttctgacct
     2821 acgactttac gttcaccgtg taccagttgt gaactccagc tcaccaccag aaaaaccacc
     2881 agcgtactga cagcacagga aaccactacc gggccaaact gcgcgcggag caaatcaaaa
     2941 tattgcatga cgcctacgcc aatcggcaca aacaatagcg ccatatagcg aatcagtacg
     3001 tagcaccccg gattgaccca tttcgccgga agaatttgca aggccagcag gacaaacagg
     3061 atcagcatcc cgatgatgct gcccggaatg gttaccggta gcagggaagc aatgaaaatg
     3121 cctgcataca ggcaggcata aatcaggacg aaagcgcgta aatattgcca gataatgttc
     3181 agtgtcttac tcataatcaa caccccagat gagatggatt catcatacaa ttaattcgta
     3241 aaacgtgcta ccgatcacat taagaaatca gcgcttattt tttcgctacc cggtattacg
     3301 caatgagcag cgaactcagg catacatcaa gcatagagag ggattgcagt ttgcagcaat
     3361 ccctctttgc agacctttaa tccgctgacc ataggcgcta acatagcgcc tcattttttt
     3421 gcgggtgatg atatgcgtgt gttactggca ccgatggagg gagtgcttga ctctctggtg
     3481 cgtgaattgc tgaccgaagt taacgactac gatctgtgca tcaccgagtt tgtccgcgtg
     3541 gtggatcaac tgctgccggt aaaagtcttt catcgcattt gccctgagct acaaaacgcc
     3601 agccggacac catctggtac gctggtgcgc gtgcagttgt taggtcagtt cccacaatgg
     3661 ctggcagaga acgccgcccg tgcggtggag ttaggtttct ggggcgtgga tctcaattgc
     3721 ggctgcccgt cgaaaacggt taacggtagc ggcggcgggg cgacgttact caaagatcct
     3781 gaactcatct accagggggc aaaagcgatg cgtgaagctg taccggcgca tttgcctgtc
     3841 agcgtgaaag tgcgtctggg ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg
     3901 gttcaacagg ctggcgctac ggagctggtg gtgcatgggc ggacgaaaga gcagggttac
     3961 cgcgcggagc atattgactg gcaggcgatt ggcgagattc gccagcggct gaatattccg
     4021 gtgattgcca acggtgaaat ctgggactgg cagagcgcgc aacaatgcat ggcgatcagc
     4081 ggctgcgacg cagtgatgat tggtcgcggg gcgctcaata ttcccaacct gagccgggtg
     4141 gtaaaatata acgaaccgcg aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat
     4201 acccgtctgg aaaagcaggg cgataccggg ttatatcacg ttgcgcggat taaacagtgg
     4261 ttgagttatt tgcgtaaaga atacgatgaa gcaacggaat tatttcagca tgttcgggtg
     4321 ttgaataact cccctgatat tgcaagggct attcaggcaa ttgatatcga gaaactctaa
     4381 aaaccatagt gatgtttttg acaatgtgtt agctctttat caataggccg cacggttgtt
     4441 tggtggcgtc aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg
     4501 gctgagggcg cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata
     4561 agccgagggc accgcgcagc ggcgattttg ttcgccagag cccggggggg cagggggcgg
     4621 cggcgattgg tcgccccctg cgcgctcctt gcgccagtga ttatatgaag caatgcacat
     4681 gacaggagcg caacaggatg agagactgaa atattctcgt tattttcgga tctgatgaca
     4741 ttaattaatt ttaaaatatc atcttaaata cgccagtcac caccagtagc ccaatcagga
     4801 aaataatcaa tacagcccag agtataattt tcattggcgt tcctttattt gtgagtgctg
     4861 aataagtata ggaaaaaacg aagccctttg ccgttatcca taaacgatta agataaatcg
     4921 gaagatatca tttacttagt ctatattttt ctgggtatgt cgttttattg ctactgatgt
     4981 attgccacgc gaaactaaaa aatatatcgc aacacacggt aatctccgcg caccgtttct
     5041 tacctgatta ttcccccatg aatcgtgatt ccttttatcc agccatcgcc tgttttccgc
     5101 tgttactgat gctggccggg tgtgcgccta tgcatgaaac ccgccaggcg ttaagccagc
     5161 aaacgcccgc tgcacaagtt gacaccgcat tacccacggc gctgaaaaat ggttggccag
     5221 acagccaatg gtggctggag tatcacgata atcagctcac ttcattaatt aacaatgcac
     5281 tacagaacgc accggatatg caggtggctg aacagcgcat ttagctagct gaagcccagg
     5341 cgaaggcagt tgccactcag gatggtccgc agatcgactt ttcggcggat atggagcggc
     5401 aaaaaatgtc ggcagaaggg ctaatggggc cgtttgctct gaacgatccg gccgcaggta
     5461 cgaccggtcc gtggtacacc aacggaactt ttggcttaac ggcgggctgg catctcgata
     5521 tctggggaaa gaatcgggcg gaggttactg cccgcctggg tacggttaaa gcacgggcgg
     5581 cggaacgcga gcaaacccgc caattgctgg ctggcagcgt agcccgcctg tactgggagt
     5641 ggcaaaccca ggcggcgtta aacacggtct tgcagcaaat agaaaaagag cagaacacca
     5701 ttatcgcgac cgatcgccag ctatatcaga acgggattac ttcttcagtt gacggtgtgg
     5761 aaaccgatat taatgccagc aaaacccggc agcagctcaa cgatgtcgcg gggaaaatga
     5821 aaattattga ggcacggtta aatgcactca caaatcacca gactaagtca ttgaagctta
     5881 aaccggtcgc gttgccgaaa gtggcaagcc agcttcctga tgaactgggg tactccttac
     5941 tggcccggcg ggcagatttg caggcggcgc actggtacgt tgagtcatcg ttaagcacta
     6001 ttgatgcggc aaaagcggca ttttatcctg acatcaacct gatggccttc ctgcaacagg
     6061 atgcgttgca cttaagcgat ctgttccgtc attccgcgca gcaaatgggc gttacggcag
     6121 gcctgacgct acctattttc gatagtggtc gtcttaacgc caatctcgat atcgcaaaag
     6181 ccgaaagcaa cttgtctatc gccagctaca acaaagcggt ggttgaagca gtgaatgacg
     6241 tggcgcgggc agccagtcag gttcagacac tggcggagaa aaaccagcat caggcgcaaa
     6301 ttgagcgcga tgccttgcgt gtggtaggtc ttgcgcaggc ccgctttaac gcgggcatca
     6361 ttgctggttc ccgcgtcagc gaagccagaa tcccggcgct gcgtgagcgg gccaatggcc
     6421 tgttattgca agggcagtgg ctggatgcct ccattcgact caccggtgcg ctgggcgggg
     6481 gttacaaacg ctgatgatat gcgccttcta tacttaacgt ttattcagcg ttaagtggag
     6541 aactcgatgg cataggtcgc gataattacc gcttccgatt cggggatcgg caaagagtgc
     6601 gcgttattac tggcgcagca ggggtttgat attggcatta cctggcactc agatgaagaa
     6661 ggggcaaaag ataccgcgcg tgaggtggtt acccacggcg tacgtgcgga gatcgtgcaa
     6721 ctggatctcg gaaatctgcc agaaggtgcg caggcgctgg agacactcat ccagcgattt
     6781 gggcgtattg atgtgctggt gaataatgcg ggtgcaatga ccaaagcgcc gtttcttgat
     6841 atggcttttg atgagtggcg caagattttt accgttgatg ttgatggtgc atttttatgc
     6901 tcgcaaattg cggctcgtca gatggtgaaa taagggcagg gcggtcgcat catcaacatt
     6961 acgtcggtac atgaacatac gccgctgccg gatgccagcg cctacacagc cgctaaacat
     7021 gcgctcggcg ggttaaccaa aacgatggcg ctggagttgg tcaggcataa gattttggtg
     7081 aacgcagtcg cgcctggggc gatagccacg ccaatgaaca gcatggatga tagcgacgtg
     7141 aagcccgacg cggagccttc gattcctcta cggcgttttg gcgcaacgca tgagattgcc
     7201 agcctggtgg tgtggctttg ttcggagggc gcaaattaca cgacagggca gtcgttgata
     7261 gtggatggcg gctttatgtt ggcgaatccg cagttcaacc cagaatagcg cagtgaggtg
     7321 cgtggatatt tcaatgggtt aggccagata agacaccagc gtcgcatcag gcatggaaca
     7381 cccggtgccg gatgtggagt gaatgcttat ctgtggcaac tgctttacgc ctgattatcc
     7441 ggctttttct tcccgcgccg tttcagccac cagcgcacgc ccaccaccag aaccacgacc
     7501 agaatcaacc agacccagtg cttcaaatgc tggtcgagat tgtgcaacca cggcgcaatc
     7561 acctgaccac cagcgtaacc aatagtggta aaaatcaacg cccaggcaaa tgcgccgaga
     7621 atattcagcg gcagaaagat tttcggcggc agctggctgg caccaatcag cgtcgggcca
     7681 atcacccgaa agccatacat aaagcgcgta ccaatgacaa acagatacgg atggcgttgg
     7741 ataagtttct gtgcccgctc aattttatcc tgatgtttcg agaaacggcg taacagcttg
     7801 ccgccaaacc gccgcccgca cagatagagc acctggtcgc caatcatgcc accaagcgcc
     7861 acagaaagta ccaccagtgg gaactttaat agcccctgat gtgccgcaac gcctcccagc
     7921 aaagtcacgg tttcaccttc cgccaggcta ccgataacca gcgcggcata accatattgt
     7981 gagatgagtg tattgagatc cataggccag tttgtctccc tttggttgcc tgtaagcata
     8041 caccctgtga atcattcacg ctgaaaaagg acatacatca gacggtgcaa attacatgga
     8101 tgaacaaaaa ataatcacaa gctggcttat acttgaactg tcaggttcat gaactcacaa
     8161 caagggggcg gtatgagcca tgtctgggga ctgttttccc atcccgatcg tgaaatgcag
     8221 gtgattaatc gcgaaaacga aacgatttct caccattaca cccaccacgt tttgctgatg
     8281 gcggcgattc cggtgatttg cgccttcatt ggcactacac agattggctg gaattttggc
     8341 gatggcacta tcctgaagtt atcctggttt accggactgg cgctggctgt cttgttttac
     8401 ggcgttatgc tggctggggt tgcggtgatg gggcgggtca tctggtggat ggcgcgcaat
     8461 tacccgcagc gtccgtctct tgcgcactgc atggtcttcg cgggctacgt tgcaactccg
     8521 ctgtttttaa gcggtctggt ggcgctttat ccactggtct ggttgtgtgc gctggtcggc
     8581 acggtggcgc tgttttacac cggctacctg ctttaccttg gtattccgag cttcctgaat
     8641 atcaacaaag aggaaggcct tagcttctcc agttcgacgc tggcgattgg cgtgctggta
     8701 ctggaagtgc tgctcaccct aaccgttata ctatggggct acggttatcg tttgttctag
     8761 aaagttcttt gacgttgcat tgctggcgta aatgtcagca atgcagcatt tactcacgat
     8821 tctcctttga cgatcgcact ttttgctcgt tatgatggcg tcgctagcct cagtaaatcc
     8881 ttactccggc ggtgcgcaac ccgtgcgcgt gaaccactat ctgaatgctc atcatgccga
     8941 aatttcgagt ttctttattt agcttggccc tgatgctggc tgtgcctttt gcaccgcagg
     9001 ccgttgcgaa aacggcagcc gctaccaccg cttcacaacc ggaaattgcc tccggtagcg
     9061 cgatgattgt tgatctgaat accaacaaag tgatctattc gaaccacccg gatctggtgc
     9121 gtccgattgc gtctatcagc aaattaatga ccgcgatggt tgtgctggat gcacgactgc
     9181 cgctggatga aaaactaaaa gtggatatca gccagacgcc ggagatgaaa ggggtctatt
     9241 cgcgcgtacg actgaatagc gaaatcagcc gtaaagatat gctgttgctg gcgctgatgt
     9301 cttcagaaaa ccgcgcggcg gcaagccttg cgcaccatta tcccggtggt tacaaagcct
     9361 ttattaaggc aatgaatgcg aaagcgaaat cgctcggaat gaacaacacg cgctttgttg
     9421 aacctaccgg attgtcggtg cataacgttt caactgcccg tgacttaacc aaactgctca
     9481 ttgccagcaa acaatatccg ttgatcgggc agttaagtac tacccgggaa gatatggcaa
     9541 ccttctctaa cccgacgtat acgctgccgt tccgcaatac taatcatctg gtgtatcgcg
     9601 ataactggaa tattcagtta accaaaaccg gctttaccaa tgcggcgggc cattgtctgg
     9661 tgatgcgtac ggttatcaat aataaaccgg tggcgctggt agtgatggac gcgtttggca
     9721 aatataccca ttttgccgat gccagccgcc tgcgtacctg gattgaaacc ggtaaagtga
     9781 tgcctgtgcc ggcagcagcg ttgagctata aaaaacaaaa agccgcccaa atggcggcgg
     9841 cggggcagac ggcacagaac gattaagtga attcggatgg cgatactctg ccatccgtaa
     9901 tttttactcc acttcctgcc agtttttccg tttactggtt ttaccgatcc ccggattcat
     9961 gctattggtc ggatcattct cgcgatagaa cttctgcaac gtctccggtg ctttatacaa
    10021 atgaccgacg ttatgctcgg cagggtactg cgcgccgcgc tgctgtagca gttccagcat
    10081 ctgttctttt aacgcatgca catccacgcc ttttttcact atgtaatcct gatggaagac
    10141 atagcacata aaatggccgt aatagagctt gtgcaccagc tggctgtcga tctccggcgg
    10201 taaatgctca taccactcgg tgtcgttacg ccgcagagcg atatccagtg ccagaatgtc
    10261 ttcgacttca tcggaatgca ccgcctgata acgaattgct gcgcccgcag cggcgaaacg
    10321 gtgtaaaaac gctttgctac cttcctccgg cgtacagaca aagaaatcgc cctcggcctg
    10381 tttgaaataa tccaccagcc acgatttggc ttcaccgacg ccatcgcctg ccatttttaa
    10441 cagcagatga tgctcgtatt tatcgcgcca gtttttcatg cgcggcggta aatggctggg
    10501 gaacaggtga ccgaattttt gcattgcacg gtcagtaaaa tgcggacgga agaatttcac
    10561 tttctccagc atcgcatcgg tgcgtccctt gagattaaag aagaacggca tcttgtcggt
    10621 gcctaactta tcaatcatca ggaaggtgtc tttgccgtat ttttccgcaa tatcgtagat
    10681 atcccggtgc atatattccc cggcaaccgg cagattttcg aagttagcca gaatatgacg
    10741 gcggatttcg gtcagcactt ccggctggtt ggtgccgata taaaacacct gctgattttt
    10801 ttccgcttcg aaggtatcaa gacgtaccgc aaagacggcc agcttcccgg cgcaaccaga
    10861 agattcaaat aaccgatccg gatcggcgtt ataacgtgcg ggcgtgtcgg cttcaatatc
    10921 acgaacgcgg tggacatagt cataatcgtg ggcgtgacga ccatcgtgac gcacatcgtc
    10981 atctttgatg cgatcgtcat ccagcttgct aaggatctgc tccggcgttt cgcccagatc
    11041 aatccccaga tggttcacca gcgtcagttt gccatcttca tctatacgcg cgaataacga
    11101 catttcggta tacgccgggc cacgttgcac cagcgagccg ccggagttgt tacaaatacc
    11161 gccgatgacc gatgcgccta tacacgatga tccaatcacc gaatgcggtt cgcgtcccag
    11221 cggtttgagg gctttttcca gcgaatagag cgtggtgccc ggataggcca gcacctgttc
    11281 gcccttgccg agaacgtgca gcttgtcgag acgcagggtg ctgatgatca cgatatcgcg
    11341 atcatagtcg ttaccgtttg gcgtcgatcc ttcggtcagg cctgtattgg cagcctgcat
    11401 cagaataatt ttgtcggcgg tgacgcaggc tttcagcacc cgccacaatt ccagtagtga
    11461 gccagggaaa acgacagcca gcgcgtcgcc ctgaccagaa cggaagccct tgcgatagcg
    11521 ggccgttttt gcgggatcgg tgagcaggtg tgaatgaccg accagacggg caagttcatt
    11581 caaaaaggct ttattatcag ttgttgtcat ggaagacatt ttccactcct tgtggtggcg
    11641 aaaaaatatc acaaactaga atagcgcctt gtatgacaat tcagcgagtg aatattttga
    11701 aaattcagac aataaactga gcattctttt tctctctcca tcatgcttat ggcaaactac
    11761 ggtttttgca ccattttcgc tgccacgtcg ggcaacaaag gaagaaaaat ccatatgaaa
    11821 tggctatgtt cagtaggaat cgcggtgagt ctggtcctgc agccagcact ggcggatgat
    11881 ttattcggta accatccatt aacgcctgaa gcgcgggatg cgttcgtcac cgatctgctc
    11941 aagaaaatga cggttgatga gaaaattggt cagctgcgct taatcagcgt aggcccggat
    12001 aatccgaaag aggcgatccg cgagatgatc aaagacggtc aggttggggc gattttcaac
    12061 accgtaaccc gtcaggatat ccgcgccatg caggatcagg tgatggaatt aagccgcctg
    12121 aaaattcctc ttttctttgc ttacgacgtg ctgcacggtc agcgcacggt gttcccgatt
    12181 agcctcggtc tggcctcgtc ttttaacctc gatgcggtga aaacggtcgg gcgtgtctct
    12241 gcttatgaag cggcagatga tggcctgaat atgacctggg caccgatggt cgatgtctcg
    12301 cgcgatccgc gctggggacg tgcttccgaa ggttttggcg aagatacgta tctcacctca
    12361 ataatgggta aaaccatggt ggaagcgatg cagggtaaaa gcccggcaga tcgttactcg
    12421 gtgatgacca gcgtcaaaca ctttgccgca tacggcgcgg tagaaggcgg taaagagtac
    12481 aacaccgtcg atatgagtcc gcagcgcctg tttaatgatt atatgccgcc gtacaaagcg
    12541 gggctggacg caggcagcgg cgcggtgatg gtggcgctga actcgctgaa cggcacgcca
    12601 gccacctccg attcctggct gctgaaagat gttctgcgcg accagtgggg ctttaaaggc
    12661 atcaccgttt ccgatcacgg tgcaatcaaa gagctgatta aacatggcac ggcggcagac
    12721 ccggaagatg cggtgcgcgt ggcgctgaaa tccggaatca acatgagtat gagcgacgag
    12781 tactactcga agtatctgcc tgggttgatt aaatccggca aagtgaccat ggcagagctg
    12841 gacgatgctg cccgccatgt actgaacgtt aaatatgata tggggttgtt taacgaccca
    12901 tacagccatt tggggccgaa agagtctgac ccggtggata ccaatgccga aagccgcctg
    12961 caccgtaaag aagcgcgtga agtggcgcgc gaaagcctgg tgttgctgaa aaaccgtctc
    13021 gaaacgttac cgctgaaaaa atcggccacc attgcggtgg ttgggccact ggcggacagt
    13081 aaacgtgacg tgatgggcag ctggtccgca gccggtgttg ccgatcaatc cgtgaccgta
    13141 ctgaccggga ttaaaaattc cgtcggtgaa aacggtaaag tgctgtatgc caaaggggcg
    13201 aacgttacca gtgacaaagg cattatcgat ttcctgaatc agtatgaaga agcggtcaaa
    13261 gtcgatccgc gttcgccgca agagatgatt gatgaagcgg tgcagacggc gaaacaatct
    13321 gatgtggtgg tggctgtagt cggtgaagca caggggatgg cgcacgaagc ctccagccgg
    13381 accgatatca ctattccgca aggccaacgt gacttgattg cggcgctgaa agccaccggt
    13441 aaaccgctgg tgctggtgct gatgaacggg cgtccgctgg cgctggtgaa agaagatcag
    13501 caggctgatg cgattctgga aacctggttt gcggggactg aaggcggtaa tgcaattgcc
    13561 gatgtattgt ttggcgatta caacccgtcc ggcaagctgc caatttcctt cccgcgttct
    13621 gtcgggcaga tcccggtgta ctacagccat ctgaacaccg gtcgtccgta taatgccgac
    13681 aagccgaaca aatatacttc gcgttatttt gatgaagcta acggagcgct ttatccgttc
    13741 ggctatggtc tgagctatac cactttcacc gtctctgatg tgaaactttc tgcgccgacc
    13801 atgaagcgtg acggcaaagt gacggccagc gtgcaggtga cgaacaccgg taagcgcgag
    13861 ggggccacgg tagtgcagat gtacttgcag gatgtgacgg cttccatgag tcgccctgtg
    13921 aaacagctga aaggctttga gaaaatcacc ctgaaaccgg gcgaaactca gactgtcagc
    13981 ttcccgatcg atattgaggc gctgaagttc tggaatcaac agatgaaata tgacgccgag
    14041 cctggcaagt tcaatgtatt tatcggcact gattccgcac gcgttaagaa aggcgagttt
    14101 gagttgctgt aatgctttag taagggcgcg acgtttgcgc cctttgcctg atgttcatat
    14161 cttaacttgc tgaattttcg ccctttgtag gccggataag gcgctcgcgc cgcatccggc
    14221 aggaaacgcc ggaccgaata tgcctcttaa gtatttatct gattctgcaa ctgaatcctt
    14281 ccgctcaggc taactctgtc attatcaact atgcttttct cttaattcgc tgaaaacaac
    14341 agtaaaagaa tgaggaaagc atcatgccac tcttaaagct ctgggcaggt tcactggtta
    14401 tgttggcagc cgtgagcctg ccgctacagg cggcttcccc cgttaaagtc ggttcaaaaa
    14461 tcgataccga aggcgcgctg ctcggcaaca tcatcttgca ggtactcgaa agccacggcg
    14521 taccgacggt gaataaagtg caactcggaa cgactcctgt ggggcgcggg gcgattactt
    14581 ccgatgaact ggatatctat ccggaatata ccggcaatgg cgctttcttc tttaaagatg
    14641 aaaacgatgc agcgtggaaa aatgccgggc agggctacga gaaagtcaaa aagctcgatg
    14701 cggagcacaa caagttaatc tggctgacgc ccgcgcctgc aaataacacc tggactatcg
    14761 ccgtgcgtca ggatgtggca gagaaaaaca aactcacttc gcttgccgac ctgagtcgtt
    14821 atctgcaaga gggcggcacc ttcaaactgg cagcctcggc agagtttatc gaacgcgccg
    14881 atgcgttacc cacgtttgaa aaagcctatg gctttaagct cggtcaggat cagttgctgt
    14941 cactggctgg tggcgacacg gcggtgacga ttaaagccgc tgcccagcaa acctctggcg
    15001 ttaatgccgc aatggcttac ggcactgacg gtccggtcgc ggcgctgggg ctgcaaacct
    15061 taagcgatcc gcaaggcgtg caacctatct acgcgcctgc accagtggtg cgtgaatcgg
    15121 tgttgaagga gtatccgcaa atggcacagt ggctacagcc agtcttcgcc agcctcgatg
    15181 caaaaacatt gcagcaactg aatgccagca ttgcagtgga aggactggat gccaaaaaag
    15241 tggctgccg
//
LOCUS       DAZSNM010000090        15196 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZSNM010000090 DAZSNM010000000
VERSION     DAZSNM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15196
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.090"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(272..1096)
                     /gene="iclR"
                     /locus_tag="ACHW42_002300"
     CDS             complement(272..1096)
                     /gene="iclR"
                     /locus_tag="ACHW42_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR8944102.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            1296..4979
                     /gene="metH"
                     /locus_tag="ACHW42_002301"
     CDS             1296..4979
                     /gene="metH"
                     /locus_tag="ACHW42_002301"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR8944103.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5199..6830
                     /locus_tag="ACHW42_002302"
     CDS             5199..6830
                     /locus_tag="ACHW42_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR8944104.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(6921..7610)
                     /gene="pepE"
                     /locus_tag="ACHW42_002303"
     CDS             complement(6921..7610)
                     /gene="pepE"
                     /locus_tag="ACHW42_002303"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR8944105.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(7992..9221)
                     /gene="sorE"
                     /locus_tag="ACHW42_002304"
     CDS             complement(7992..9221)
                     /gene="sorE"
                     /locus_tag="ACHW42_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFR8944106.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            complement(9273..10061)
                     /locus_tag="ACHW42_002305"
     CDS             complement(9273..10061)
                     /locus_tag="ACHW42_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IID"
                     /protein_id="HFR8944107.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVSLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            complement(10072..10869)
                     /locus_tag="ACHW42_002306"
     CDS             complement(10072..10869)
                     /locus_tag="ACHW42_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR8944108.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            complement(10935..11426)
                     /locus_tag="ACHW42_002307"
     CDS             complement(10935..11426)
                     /locus_tag="ACHW42_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFR8944109.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQL
                     IAN"
     gene            complement(11426..11833)
                     /locus_tag="ACHW42_002308"
     CDS             complement(11426..11833)
                     /locus_tag="ACHW42_002308"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR8944110.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            complement(11843..12649)
                     /locus_tag="ACHW42_002309"
     CDS             complement(11843..12649)
                     /locus_tag="ACHW42_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8944111.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(12719..13666)
                     /locus_tag="ACHW42_002310"
     CDS             complement(12719..13666)
                     /locus_tag="ACHW42_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFR8944112.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            14014..14886
                     /gene="rluF"
                     /locus_tag="ACHW42_002311"
     CDS             14014..14886
                     /gene="rluF"
                     /locus_tag="ACHW42_002311"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR8944113.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(15019..>15196)
                     /gene="yjbD"
                     /locus_tag="ACHW42_002312"
     CDS             complement(15019..>15196)
                     /gene="yjbD"
                     /locus_tag="ACHW42_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR8944114.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
BASE COUNT         3631 a         3953 c         3996 g         3616 t
ORIGIN      
        1 aacagtgaaa aaatttaata tgattacaac taaaaaaata tcataaatcg ctcaatctca
       61 taatgcagcc gtaaaagttt cggtggaatg agatcttgcg attttgttaa taattaagtg
      121 gtttgatgtt taaaaataat gctgagttaa taaaagtgtg tgaagtgtat gacattaagt
      181 tattttggcg tcaatgcgat taacagacac ccttattcta ttgccactca ggtatgatgg
      241 gcagaatatt gcctctgccc gccagaaaaa gtcagcgcat tccaccgtac gccagcgtca
      301 cttccttcgc cgctttaatc accatcgcgc caaactcggt cacgcgatca tcggtaatac
      361 gtgaaatcgg tccggaaata gaaattgcgg caaacggttc gcggtgctca tcgaaaatac
      421 acgctgcaag gcaacgtagc cccagcgcat gttcctcatc gtcaaatgaa taaccccgtt
      481 tgcgcgtttg ggcgagatct tcttttaaat gcacaggaga caccagagtt gcgtgggtat
      541 aggcatgtaa ccctttgcgg tgcagcagct tcgtcacctg ttcttcgctc agttgggcta
      601 aaaaggcttt acccgcaccg gaagcgtgca tcggcaattt accgccgata ggcgcggaca
      661 ttcgcatcag gtgggtacac tgtacctggt cgataataat cgcttcgtga tcgctttgat
      721 caagcaccgc catattgacc gtttcgccag actcttccat tagattgcgc aggatagggt
      781 gaacaattgc taacaaatta cggctctgga gaaagctgct gccgaccata aaggcatgtg
      841 cgccgattgc ccagtgtccc agttcgccga cctgacgcac gaaaccctgc tgttgcatcg
      901 tggttagcag gcggtgggtc gtggaattgg gtaacccggc ttgttgcgcc agctctgtga
      961 gtgccacgct gccattggat tcggcaatcc actccagtaa tttcaggcca cgcgttaaag
     1021 actgaacctg tccagtcgct ggtgcggtgg caacggcggg ttttctgccg cgtttcgcgg
     1081 gaatgggtgc gaccatgaca gcctcctttt tctgtatcgt ggaaattatt ttcattttta
     1141 ttgttagcta atgcaatagt tgctgaactg atccgatgag ttaatgttga acaaatctca
     1201 tgttgcgtgg tggtcgcttt taccacagat gcgtttattc cagtatggtt tgttgaattt
     1261 ttattaaatc tgggttaagc gtgtcgggag caagtgtgag cagcaaagtg gaacaactgc
     1321 gtgcgcagtt aaatgaacgt attctggtgt tggacggcgg tatgggcacc atgatccaga
     1381 gctatcgact gaacgaagcc gattttcgtg gtgaacgctt tgccgactgg ccatgcgacc
     1441 tcaaaggcaa caacgacctg ctggtactca gtaaaccgga agtgatcgcc gctatccaca
     1501 acgcctactt tgaagcgggc gcggatatca tcgaaaccaa caccttcaac tccacgacca
     1561 ttgcgatggc ggattaccag atggaatccc tgtcggcgga aatcaacttt gcggcggcga
     1621 aactggcgcg cgcctgtgct gacgagtgga ccgcgcgcac gccggagaaa ccgcgctacg
     1681 ttgccggtgt tcttggccca accaaccgca cggcgtctat ttctccggac gtcaacgatc
     1741 cggcattccg caacatcact tttgaccagc tggcggcggc ctatcgcgaa tccaccaaag
     1801 cgctggtgga aggtggtgcg gatctgatcc tgattgaaac cgttttcgac acccttaacg
     1861 ccaaagcggc ggtctttgcg gtgaaaacgg agtttgaagc gctgggcgtt gagctgccga
     1921 ttatgatctc cggcaccatc accgacgcct ccgggcgcac gctctccggg cagaccaccg
     1981 aagcatttta caactcattg cgccacgccg aagcgctgac ctttggcctg aactgtgcgc
     2041 tggggcccga tgaactgcgc cagtacgtgc aggaactatc acggattgcg gaatgctacg
     2101 tcaccgccca cccgaacgcc gggctgccta acgcctttgg tgaatacgat ctcgacgccg
     2161 acacgatggc aaaacagata cgtgaatggg cgcaagcggg ctttctcaat atcgtcggtg
     2221 gttgctgcgg gaccacgccg caacatattg cagcgatgag tcgtgcagta gaaggattag
     2281 cgccgcgcaa actgccggaa attcccgtag cctgccgttt gtctggcctg gagccgctga
     2341 acattggcga cgatagcctg tttgtgaacg tgggtgaacg caccaacgtc accggctctg
     2401 ctaagttcaa gcgcctgatc aaagaagaga aatacagcga ggcgctggat gtggcgcgtc
     2461 agcaggtgga aaacggcgcg cagattatcg atatcaacat ggatgaaggg atgctcgatg
     2521 ccgaagcggc gatggtgcgt tttctcaatc tgattgccgg tgaaccggat atcgctcgcg
     2581 tgccgattat gatcgactcc tcaaaatggg atgtcattga aaaaggtctg aagtgtatcc
     2641 agggcaaagg cattgttaac tctatctcga tgaaagaggg cgtcgacgcc tttatccatc
     2701 acgcgaaatt gttgcgtcgc tacggtgcgg cagtggtggt gatggccttt gacgaacagg
     2761 gacaggccga tactcgcgca cggaaaatcg agatttgccg tcgggcgtac aaaatcctca
     2821 ccgaagaggt tggcttcccg ccagaagata tcatcttcga cccgaacatc ttcgcggtcg
     2881 ccaccggtat tgaagagcac aacaactacg cgcaggactt tatcggcgcg tgtgaagaca
     2941 tcaaacgcga actgccgcac gcgctgattt ccggcggcgt atctaacgtt tctttctcat
     3001 tccgtggcaa cgatccggtg cgcgaagcca ttcacgcagt gttcctctac tacgctattc
     3061 gcaatggcat ggatatgggg atcgtcaacg ccgggcaact ggcgatttac gacgacctac
     3121 ccgctgaact gcgcgacgcg gtggaagatg tgattcttaa tcgtcgtgac gatggcactg
     3181 agcgtttact ggagcttgcc gagaaatatc gcggcagtaa agcggacgac actgctaacg
     3241 cccagcaagc ggagtggcgc tcgtgggacg tgaataaacg tctggaatac tcgctggtca
     3301 aaggcataac cgagtttatc gagcaggata ctgaagaagc ccgccagcag gctacgcgcc
     3361 cgattgaagt gattgaaggc ccgttgatgg acggcatgaa cgtggtcggc gatctgtttg
     3421 gcgaaggcaa aatgttcctg ccacaggtgg tcaaatcggc gcgcgtcatg aaacaggcag
     3481 tggcgtatct cgaaccgttt attgaagcca gcaaagagca gggtaaaacc aacggcaaga
     3541 tggtgatcgc caccgtgaaa ggcgatgtcc acgacatcgg taaaaatatc gttggtgtgg
     3601 tgctgcaatg taacaactac gaaattgtcg atctcggcgt tatggtgcct gcggaaaaaa
     3661 ttctccgtat cgccaaagaa gtgaatgctg atttgattgg cctttcgggg cttatcacgc
     3721 cgtcgctgga cgagatggtt aacgtggcga aagagatgga gcgtcagggc ttcgccattc
     3781 cgttactgat tggcggcgcg accacctcaa aagcgcacac ggcggtgaaa atcgagcaga
     3841 actacagcgg ccctacggtg tatgtgcaga atgcctcgcg caccgttggt gtggtagcgg
     3901 cgctgctttc cgatactcag cgtgatgatt ttgtcgctcg tacctgcaag gagtacgaaa
     3961 ccgtgcgtat tcagcacggg cgcaagaaac cacgcacacc accggtcacg ctggaagcgg
     4021 cgcgcgataa cgatttcgca tttgactggc aggcttacac gccgccggtg gcgcaccgtc
     4081 tcggcgtgca ggaagtggaa gccagcatcg aaacgctgcg taattacatc gactggacac
     4141 cgttctttat gacctggtcg ctggccggga agtatcctcg cattctggaa gatgaagtgg
     4201 tgggcgttga ggcgcagcgg ctgtttaaag acgccaacga catgctggat aaattaagcg
     4261 ccgagaaaac gctgaatccg cgtggcgtgg tgggcctgtt cccggcaaac cgtgtgggcg
     4321 atgacattga aatctaccgt gacgaaacgc gcacccatgt gatcaacgtc agccaccatc
     4381 tgcgtcaaca gaccgaaaaa acgggattcg ctaactactg tctcgctgac ttcgttgcgc
     4441 cgaagctttc tggtaaagca gattacatcg gcgcatttgc cgtgactggc gggctggaag
     4501 aggacgcact ggctgatgcc tttgaagcgc agcacgatga ttacaacaaa atcatggtga
     4561 aagcgcttgc cgaccgttta gccgaagcct ttgcggagta tctccatgag cgtgtgcgta
     4621 aagtctactg gggctatgcg ccgaacgaga acctcagcaa cgaagaactg atccgcgaaa
     4681 actaccaggg catccgtccg gcaccgggct atccggcctg cccggaacat acggaaaaag
     4741 ccaccatctg ggagctgctg gaagtggaaa aacatactgg catgaaactc acagaatctt
     4801 tcgcgatgtg gcctggtgca tcggtttcgg gttggtattt cagtcatccg gacagcaagt
     4861 actacgcggt ggcgcaaatt cagcgcgatc aggttgaaga ccatgcccgc cgtaaaggta
     4921 tgagcgtctc cgatgttgag cgctggctgg caccgaatct ggggtatgac gcggactgat
     4981 tcacaaatct gtcacttttc cttacaacaa acagggcgct caatgagtgc cctgtctctt
     5041 tattaatatg aaacacttat actggaaaca ggctggaata atcttagccg gaaaaacgaa
     5101 tgtcaaaagg cacagtaaag atgccagtca gcgtgtttcc ggtcctttgg tcattcaatt
     5161 gcttcaggaa catataacga taaggagaat ctgaactcgt gttaacgctg cttcacctgc
     5221 tttctgccgt cgccctgctg gtctggggga ctcatattgt tcgaaccggc gtaatgcgcg
     5281 tcttcggcgc gcgtttgcgt actgtcctta gccggagcgt cgaaaaaaag ccgctcgcct
     5341 tttgcgcggg gatcggcgtt accgcactgg tacagagcag taatgccacc accatgctgg
     5401 tgacctcgtt cgtcgctcag gatctggtag ccctcgcacc ggctctggtc attgtgctgg
     5461 gggcagatgt cgggacggcg ctaatggcgc gtattctcac cttcgactta tcctggctgt
     5521 caccgttact tatttttatc ggcgtgattt ttttcctcgg acgcaaacag tcacgcgccg
     5581 ggcaactggg ccgcgtcggt attggtcttg ggctgatttt gctggcgctg gagttgattg
     5641 tgcaggccgt aacgccgatc acccaggcaa acggcgttca ggtgatcttt gcctcgctga
     5701 ccggcgatat tctgctggat gcgctgattg gcgcgatgtt cgccattatc agctactcca
     5761 gccttgctgc tgtactgctg accgcgactc tgaccgctgc aggcattatc tccttccccg
     5821 tggcgctctg tctggtgatt ggagctaacc tcggttccgg cctgctggcg atgctcaaca
     5881 acagtgccgc caatgccgca gcccgccgtg tcgcgctggg tagtctgctg tttaagctgg
     5941 tgggtagcct gattatcctg ccgtttgtcc atttgctggc agagacaatg gggaagctgt
     6001 cattgccaaa agcggaactg gtgatctatt tccacgtctt ctacaacctt gtacgttgcc
     6061 tggtcatgct gccatttgtt gacccgatgg cacggttttg caaaacgatt attcgcgatg
     6121 aaccggaact ggatacccag ctacggccta aacatctgga tgtcagcgcg ctggacacgc
     6181 ccacgcttgc tctggcgaac gccgcgcgcg aaaccctgcg cattggcgac gccatggaac
     6241 agatgatgga agggctgaat aaagtgatgc acggcgagcc acggcaggag aaagagctgc
     6301 gtaagctggc agatgatatc aacgttctct acaccgccat taagctgtat ctggcgcgga
     6361 tgccaaaaga agagttggcg gaggaagagt cgcgccgctg ggcggagatc atcgaaatgt
     6421 cgctcaacct tgaacaggcc tccgatatcg tcgagcgcat gggcagcgaa attgccgata
     6481 aatcgctggc agcacggcgg gcattttcgc ttgatgggtt gaaggaactg gatgcgctct
     6541 atgagcaatt gctcagtaat ttaaagctgg caatgtcggt gttcttctct ggcgatgtca
     6601 ccagcgctcg tcgtttgcgt cgcagcaaac atcgttttcg cattcttaat cgccgctatt
     6661 cccacgccca cgtcgatcgc ctgcatcagc aaaacgtgca gagcattgaa accagttcgc
     6721 tacatttagg cttactggga gatatgcagc gcctgaactc gctgttttgt tcggtggctt
     6781 acagtgtgct ggaacagccg gatgaagatg aaggacggga cgagtattaa tattgtgaat
     6841 cctgtaggcc tgataagacg cgtcaagcgt cgcatcaggc actatgaaca agatgccgga
     6901 tcatgcatcc ggcaattcac ttaaaaacgg tgaccagctt ccagcggaac cgcttcttca
     6961 ccagccttaa acacataagt ggtgttcggg ccacccagcg tagcgtgacc tttactcact
     7021 gtgatccagt taccttccgg cagaccaata atcgtcagtt ctggcgcgac gaccagcagt
     7081 tcgcgaatac gctgctcacg ggtttcacct ttatggcctt ccggcagcgc gttggtgaag
     7141 tgcgggttga tttgcaacgg gaacagattt agcgcatcga aaccttgcgg atcgacaatc
     7201 ggcatatcgt tggtggtacg aatggttggg caagcaaggt tagcgcctgc gctccagcca
     7261 atatacagcg cgccacgttt aaccacgtca gtaattggag ccagcagccc gcgctcgcgg
     7321 cactgtttca gcaactggaa agtattaccg ccgccgacaa tcacaatttc cgcattttca
     7381 atcgcggcaa cgggatcgac aacgctatga ataccggtga cagaaacacc cagcggagcg
     7441 agaaccgcag ccgttttctc tgtgtaatca tcccaggtct gcgttacgcc agcgaaaggg
     7501 ataaacaccg ctgagcggcg accctgcaac tgttcagcaa ttagcggcag tgcatgttcc
     7561 agccaggctt tacccggcag cgtcgagtta ctcaataaaa gcagttccat tacttctccg
     7621 gttagtaaga aatcacggaa ggcatgctac cactgcgaat ttaaccactt actgacatgg
     7681 ctcacgatgt cacgtattgc accgggcgct gagactgatg acaaacacag cattgcctga
     7741 tgcgctacgc ttacctggcc taccagataa ctgcaatata ttggattttc aaaattatgt
     7801 agaccgaata aggcttcatt ccgcatccgg cataaacaat gtgtactttg ccaccaattt
     7861 gagcgcccct taaggcgctg atatttttta tccttctcgt gcaaattgtg ctgcttcagc
     7921 aatcagccgc acatccggtc gcgaagttgc tggggcgacc agccagcgac atgcagagtt
     7981 aagtagcgcg attaatcatg ggaaattgcc tctgcatggg cgagcaggta ttgctccacc
     8041 tcaccggacc agatcccctg atgacgcgcc agaatcgccg ccagtgcttg atcctgaatc
     8101 tgcgtgagtg acgtcagcgg caggtatttc ccggtataca ccagcttttt gccgccgccg
     8161 acggcaggca attcaagcgt ggtttcgccc gcggcattca gcccaagaat atgtgttacc
     8221 acttttgcgg cctgcacttt tttctcttcg atcagtttga ccgccgcgcg catgtcgtcg
     8281 gtattgccgc ctgacgtgcc cacgtagtgg gtaaatgcat aatgcacatc gtagaaatta
     8341 attggcgcgc tgaaatgttt atcctgcggt ccggcgaaga aattcaggca accatctgtc
     8401 gccagcaagg aagaggcgag agtcaccagt ccttcattag gcacaaagac gaaaatatca
     8461 tcgaagccgt gaccgccact cagcgccatt aacgtatcaa atgctgcatc ggtggcattg
     8521 agataatgaa tcagtgtttg cggttctgat ggatagtgct tgcgcgcata actcaatttg
     8581 tcgttgtcgg tatcggtaat gacgagcagc gacgggttaa ctggtccatg cagcgcataa
     8641 tcaatcgcca acagtcccat tggtccggtg ccgccgagga tcagcgtgtg cccttgcggg
     8701 cgaatcccca tcgtgtggtt ataactacct tcctgaagat gatagttggc gttgaacgcg
     8761 ccaatcacgc aggaaagcgg ttcaaccagt gagccttcaa aataggtttc gccgtcgtat
     8821 gccagcaggc aatattgttc catgacctcg ttgggaataa ccacatgcgt cgcttcgccg
     8881 tctacccacg ggaaggagta gccggggcaa tccgggcggt cggggagttg caggttggcc
     8941 tgaatcacat aacgctgacc cggctggaat ttgtgctgcc actttttacc cacggccaga
     9001 atatcgccgc aaaattcgtg gccgatgatg attgggttgg tcgtcacatc gtcgggtact
     9061 tttttatggt tttcacccag attggcctct ttccaggaag agaggcacag gctgtcagtg
     9121 accaccgttg cgaggatttc atcctcctgc atttcaggaa ggtcaaaggt ttccaggcgt
     9181 aaatcacgtt taccataaag acgcagagct gttgttttca taaaccacct catgttgagc
     9241 gatggcctcg gcagtcgtgc cggggcgaag aatcaggaca ggaagcccag agcattgccg
     9301 ataatcccta agccaaacag ggcgaaaatc agccataccg ggttaatttt tttgttgagc
     9361 agacgaacca ttagtagcgt cagaccgagc gccagcaaac cagggcaaag ttggtcgaga
     9421 atgttctgca cggtcatggt gacggtggag ccatcggcgg catgcgtttg tgaaaccacc
     9481 aacggtacgt tgattgacgt ccatttggtc accagcacgc ccatcacaaa cagtccgaga
     9541 atcgacgcgc cttcggtgag tttttgcagc acattcccgc ccatatcgct gacgatattc
     9601 acccctttgc gaaagccgag ctgtaggcca taccacttca tcgccagact caccgcgttg
     9661 aaaataaaga agaacagcag cgggccgaga atgttgcccg aaagtgccag agatgcgccg
     9721 agcgcggcgg taatcgggcg cagcgttccc cagaccagtg gatcgccaac tcctgccaat
     9781 ggtcccatca gaccgacttt gatgccgttg atggtaccgt catcaatttc cgcgccgatg
     9841 accgggccac atacggctgg cgtggtattg aagaacacca ggtgtagcct gagcgccgca
     9901 acctgatcct cttttaatgg gtaaagtcgc ttgatggcgg ggatcatgtc gtagcaaaag
     9961 cccagcccgt gaatacgttc aaagttaaag gacgcctgtt gcaggttgga gcgcagaaac
    10021 atgctcacca gatcgctgcg tgtaattttt ctctgttcca tgatggctcc gttaatcgtc
    10081 aagttggtca agggcggtgg aggcggtggc ctggagttga ggttcagctt tacaccactg
    10141 tggatttagc tggatgtaga ccagggccat gatcacgccg acgccaccga acgccagcag
    10201 gctgagatcg aggtagccac ctgcgaggaa accgaggaaa aagaaaggca tcaaatattt
    10261 cacgcccatc atacgtagca ccatggcgta accgaccacc acgataaaac cgccagcaat
    10321 ctgtagtcct cgggtcacaa attcgggaat ggcgtttaac atattgctga ccatatcggc
    10381 gctgacgaac agtgagacaa tcagtgccgg aatggcgacg cgcagcgcct gcacgccaag
    10441 cgcggagaca tgcagaatat cgagagtgcg aaaccgcgcc tcttctgctg ctttatccgc
    10501 cgcgtgctgg aacactacgg tgatggtacg ggcaaacacc gtcagcacct ggcctgccgc
    10561 agccacaggc aacgcgatgg cgattccggt ggcgatgctt tgctgaccaa cgataaccag
    10621 aatggcggag attatgctgg cgagtgcaga atccggagat tgcgccgcgc cgacgttcat
    10681 ccagcagaga gctatcaatt ccagcgtacc gccgagtatt attccggttt ttaaatcacc
    10741 gagaattaaa ccaatcaccg tacaggcaat taacgggcga tgagtctgaa attcatccag
    10801 cacgctgccc attccggcaa tacaggaaaa aagaaatata gcaatgattt gtagggtact
    10861 tatttccatg atttgtcacc ttttaagtgt cgtcatccct gcctgttaaa gcatatgtac
    10921 acaggcgcta ttatttaatt tgcgattaac tgttcgttta ttttgtcgat aatattgatt
    10981 gagggatctg acgctacgac gcgtaaatcc agtatgacac caagattatt taactcatga
    11041 aatgcgttaa tatcatcatc taatgaaacc gctttggtta actgtttttt accgggccgc
    11101 caggccatgc cgccaatatt cagggtgcct atttttaccc cctggcgaac catcgctaag
    11161 gcatcctgtg ggcgagtgaa aagataaaaa acggtttcgt cctgatattg cgggttgtga
    11221 taaaccgcga cggctttttc aatattgacc acgttaacct tcattcccgg tggcgcagcc
    11281 tgacgcaata atgtccggcg cacttcatcg ttataaactt cgtcattgca gataataatg
    11341 cgctgggcgt tagccacttt cgaccagacg gtagtgacct ggccgtggat cagacggtca
    11401 tcaatgcggg cgagcgtaat gttcatcaga aatcctcctc tgtcgttgcc agttgtttcc
    11461 agacaacgca ggtttgcttt gcgatttgcg tcaggtgttc acacagttca tcaacattca
    11521 tgctgtcctg gttatcaacc agttcgagcg ccagcggcag agaaaggccg ctgattaccc
    11581 gcaaacgagg attgcgcatc gcaagcattg ctgccgcatt ccacgggctg ccgcactgta
    11641 aatccacggc aatcagccac tcatcttgat tgtgaatgct tactaacttt tccagcttta
    11701 ccgcaatgtc tccggcgttt tcaccgggca caaatgcaac ggcttcaaca ttggcgttgc
    11761 cgtaaaccat ctgaaccgat tccagcatgg cgcaagctaa tttgccgtgg gcacaaaaaa
    11821 tggcgttgac cataaagcct ccttagccgc gcgttttgcc gcccgcaatg ttagtggtta
    11881 ctccggtgat gtagctggcg cgttcagaca acagataaca aacaaaatca gctacttctg
    11941 ctaatcttcc ggcgcgccca ataggaatgg cgtttttggt atagccttca cgcagctgct
    12001 cgacggtgat attgcgcgtc cacgccagcg cctcttcata ttccggcgtg cgtagcccgg
    12061 ttttttccag aatcccgggc gcgataccga ccacacggat accgtgttta cccagctctt
    12121 tcgaccagga gcgcgtgaag ctattgagcg cggctttggt agcggcgtaa cagctttgtc
    12181 cttctgagcc ttccagccca ctttctgagg aaacattcac aatcacgcca tcatgttgtt
    12241 tgaccatctg tcgcgccacc gcctgcgaca tcagaaaaac acctttctgg ttgatattga
    12301 ccattttttc gaatgcagct tcgttgagtt catactgccc ggcaggcgct ttctcatcga
    12361 ccagcagacg cgggaaattg accccggcgt tattgaccag accgtcgatg cgaccaaaac
    12421 gctggataat ttccgctacc gtatgattta cctcttttgt gctagaaata tcggtcggcc
    12481 aaaactgata gcctttatga ccttcatatt gaccatcgcc accgtgaata tcgaccatct
    12541 gtacattcgc gccttgtgct aataattcct ctacaatcgc cagaccaata ccggatgcgc
    12601 cgccggtgac aataatgatt ttatcctgca aatttaacca cgtttgcata acagactcct
    12661 gattttagat aatagggacc cgccgtaatt tttattacgg taaacatcgg gaagattttt
    12721 atttcagtaa tagttcagct gtgctgctat tcgttaccag acaattaata tattttccac
    12781 gcagtgcacc aataattccg ctgtattttt cttcactcat ggcaatgcca atggaatatc
    12841 gcgcctgctt taatttattc atttcgatag agagcgtttt ttcgctcata ttcgtttcaa
    12901 ccattgcgcc gtgaatatca aaaaagcgcg agcaaatatc gccagcaacc tggcgggcat
    12961 tcaggtcgtc actctcttca ccaccataaa acgcatgcca gttagcgccg tcgcgaatgg
    13021 ccggtgagcc aattcccacc agggcgatat ccagattatc ccagtaggca gagatggttt
    13081 taaagtgctg agactgcatg atcccattac gaattaatgg gttatccagc agagccggaa
    13141 aatccgcgag atgcgattcc cctttcagct tcgctgccgc gctgtaggtt aatgtgttta
    13201 catgatagcg gctttcgagt ttaccggacg gtccgccaat aattggtacg cagattaact
    13261 gccgcgattg ccccgcctgc ggcaagtttt caaccagtgc gctcaccgcg cgaccccagg
    13321 aaaaaccgac aatatcgcca ggctccagca agcgatccag cagttgcgcg ccgtgtaacc
    13381 ccatcatcgc cagttgagtc tcttcatcct catcatttcc cgacaccacc acaacgtctt
    13441 tcaggccaaa cttttgcttc agttgctgct ccagccagag attttcgttg tagtcatagt
    13501 tgatggcgat ggtgaccatt ccctgatcgc ggcctcgttt aagcaagcgg ctgatggtgg
    13561 tgcggtaaat acccagttcg cgcgcgattt gcgcctgcgt catatcctgt tcgtaataga
    13621 gttgggcaat cttcacaatc aaacggatat cgtcactgtt ttccattgcc gcctcctggt
    13681 tctgcacatt tgtgcaaata cgctttctca ccgcttgatc tgatataacc ccatcatgag
    13741 ggcctttcaa agcgattttt cactctttgc atggggagaa atagcgcttt ctcttgcaca
    13801 tttgtttctc tgcgtagtag tcatcagcac aggtgtgcag cgattcgtaa tatgttgatt
    13861 atccttattt tgccaggcaa atttgcatcg tacttccgga gacacttttg tgataaaagg
    13921 cgtggcagat cacatttaat ttcgacgttg ccgcataacc ctgtttttcc ccgctaagag
    13981 gtacggtata ttccgcgttt tacaggagaa tttatgctgc ccgactcatc agtccgttta
    14041 aataaataca tcagcgaaag cggaatttgc tcacgccgcg aagcggatcg ctatatcgag
    14101 caaggcaatg tgttccttaa tggcaagcga gccaccattg gcgatcaggt gaaacccggc
    14161 gacattgtga aagtaaacgg tcagttgatt gagcctcggg aagccgaaga tttggtactt
    14221 atcgccctga acaagcccgt cggtattgtc agcaccaccg aagatggcga gcgcgataac
    14281 attgtcgatt tcgttaacca cagcaaacgc gtgttcccga ttggccgcct ggataaagac
    14341 tcccaggggc tgattttcct caccaatcac ggcgatctgg tgaataagat cctccgtgcg
    14401 ggtaacgatc atgagaaaga gtatctggtg acggtcgata aaccgattac cgatgaattt
    14461 attcgtggta tgggggcggg ggtgccgatc ctcgggacgg tgacgaaaaa gtgcaaagtc
    14521 aaaaaagaag cgccgtttgt cttccgcatt accctagtgc aggggctgaa ccgtcagatc
    14581 cggcgcatgt gcgagcattt cggctatgaa gtgaaaaagc tggaacgtac gcgcattatg
    14641 aacgtcagct taagcggcat tccgctgggg gagtggcgcg atttaaccga cgatgagtta
    14701 atcgacctct ttaagctcat cgaaaattcc tcttccgagg caaaacctaa agcgaaggcc
    14761 aaaccgaaaa cagcgggcat caaacgtcca gtcgttaaga tggaaaaaac ggcggaaaaa
    14821 ggcggtcgcc cggcgtccaa cggtaagcgt tttacctcgc cggggcgtaa aaagaaggga
    14881 cgctgattaa cgcctcagac tgatgaaaac gtccaaaaag agctcggaca gtcccctcgc
    14941 cccttcgggg agagggttag ggtgagggga agattgtgtc cacacagacg agtgaaaatc
    15001 tcaggcgttg attgttcatt agcgcctgcc gcgcgtcgac gtatttgctg accagctaaa
    15061 tgacgcttca ggatccggtt tataagcctg ctttttcttc aattggcggg cttttttcgc
    15121 ttcagcctca cgctcgacca ttaatttatc gatgtactct ttcttaatgc tgttggtttc
    15181 ggagttggtc agtacg
//
LOCUS       DAZSNM010000091        15129 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZSNM010000091 DAZSNM010000000
VERSION     DAZSNM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15129
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.091"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            70..546
                     /gene="bfr"
                     /locus_tag="ACHW42_002313"
     CDS             70..546
                     /gene="bfr"
                     /locus_tag="ACHW42_002313"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFR8944115.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(543..1010)
                     /locus_tag="ACHW42_002314"
                     /pseudo
     CDS             complement(543..1010)
                     /locus_tag="ACHW42_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
     gene            1389..1700
                     /gene="rpsJ"
                     /locus_tag="ACHW42_002315"
     CDS             1389..1700
                     /gene="rpsJ"
                     /locus_tag="ACHW42_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR8944116.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1733..2362
                     /gene="rplC"
                     /locus_tag="ACHW42_002316"
     CDS             1733..2362
                     /gene="rplC"
                     /locus_tag="ACHW42_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR8944117.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2373..2978
                     /gene="rplD"
                     /locus_tag="ACHW42_002317"
     CDS             2373..2978
                     /gene="rplD"
                     /locus_tag="ACHW42_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR8944118.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2975..3277
                     /gene="rplW"
                     /locus_tag="ACHW42_002318"
     CDS             2975..3277
                     /gene="rplW"
                     /locus_tag="ACHW42_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR8944119.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3295..4116
                     /gene="rplB"
                     /locus_tag="ACHW42_002319"
     CDS             3295..4116
                     /gene="rplB"
                     /locus_tag="ACHW42_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR8944120.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4133..4411
                     /gene="rpsS"
                     /locus_tag="ACHW42_002320"
     CDS             4133..4411
                     /gene="rpsS"
                     /locus_tag="ACHW42_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR8944121.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4426..4758
                     /gene="rplV"
                     /locus_tag="ACHW42_002321"
     CDS             4426..4758
                     /gene="rplV"
                     /locus_tag="ACHW42_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR8944122.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4776..5477
                     /gene="rpsC"
                     /locus_tag="ACHW42_002322"
     CDS             4776..5477
                     /gene="rpsC"
                     /locus_tag="ACHW42_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR8944123.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5490..5900
                     /gene="rplP"
                     /locus_tag="ACHW42_002323"
     CDS             5490..5900
                     /gene="rplP"
                     /locus_tag="ACHW42_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR8944124.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5900..6091
                     /gene="rpmC"
                     /locus_tag="ACHW42_002324"
     CDS             5900..6091
                     /gene="rpmC"
                     /locus_tag="ACHW42_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR8944125.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6091..6345
                     /gene="rpsQ"
                     /locus_tag="ACHW42_002325"
     CDS             6091..6345
                     /gene="rpsQ"
                     /locus_tag="ACHW42_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR8944126.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6510..6881
                     /gene="rplN"
                     /locus_tag="ACHW42_002326"
     CDS             6510..6881
                     /gene="rplN"
                     /locus_tag="ACHW42_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR8944127.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6892..7206
                     /gene="rplX"
                     /locus_tag="ACHW42_002327"
     CDS             6892..7206
                     /gene="rplX"
                     /locus_tag="ACHW42_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR8944128.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7221..7760
                     /gene="rplE"
                     /locus_tag="ACHW42_002328"
     CDS             7221..7760
                     /gene="rplE"
                     /locus_tag="ACHW42_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR8944129.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7775..8080
                     /gene="rpsN"
                     /locus_tag="ACHW42_002329"
     CDS             7775..8080
                     /gene="rpsN"
                     /locus_tag="ACHW42_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR8944130.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8114..8506
                     /gene="rpsH"
                     /locus_tag="ACHW42_002330"
     CDS             8114..8506
                     /gene="rpsH"
                     /locus_tag="ACHW42_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR8944131.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8519..9052
                     /gene="rplF"
                     /locus_tag="ACHW42_002331"
     CDS             8519..9052
                     /gene="rplF"
                     /locus_tag="ACHW42_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR8944132.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9062..9415
                     /gene="rplR"
                     /locus_tag="ACHW42_002332"
     CDS             9062..9415
                     /gene="rplR"
                     /locus_tag="ACHW42_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR8944133.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9430..9933
                     /gene="rpsE"
                     /locus_tag="ACHW42_002333"
     CDS             9430..9933
                     /gene="rpsE"
                     /locus_tag="ACHW42_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR8944134.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9937..10116
                     /gene="rpmD"
                     /locus_tag="ACHW42_002334"
     CDS             9937..10116
                     /gene="rpmD"
                     /locus_tag="ACHW42_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR8944135.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10120..10554
                     /gene="rplO"
                     /locus_tag="ACHW42_002335"
     CDS             10120..10554
                     /gene="rplO"
                     /locus_tag="ACHW42_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR8944136.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10562..11893
                     /gene="secY"
                     /locus_tag="ACHW42_002336"
     CDS             10562..11893
                     /gene="secY"
                     /locus_tag="ACHW42_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR8944137.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11925..12041
                     /gene="rpmJ"
                     /locus_tag="ACHW42_002337"
     CDS             11925..12041
                     /gene="rpmJ"
                     /locus_tag="ACHW42_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR8944138.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12188..12544
                     /gene="rpsM"
                     /locus_tag="ACHW42_002338"
     CDS             12188..12544
                     /gene="rpsM"
                     /locus_tag="ACHW42_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR8944139.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12561..12950
                     /gene="rpsK"
                     /locus_tag="ACHW42_002339"
     CDS             12561..12950
                     /gene="rpsK"
                     /locus_tag="ACHW42_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR8944140.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRVLNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12984..13604
                     /gene="rpsD"
                     /locus_tag="ACHW42_002340"
     CDS             12984..13604
                     /gene="rpsD"
                     /locus_tag="ACHW42_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR8944141.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13630..14619
                     /gene="rpoA"
                     /locus_tag="ACHW42_002341"
     CDS             13630..14619
                     /gene="rpoA"
                     /locus_tag="ACHW42_002341"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR8944142.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14660..15043
                     /gene="rplQ"
                     /locus_tag="ACHW42_002342"
     CDS             14660..15043
                     /gene="rplQ"
                     /locus_tag="ACHW42_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR8944143.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         3899 a         3566 c         4036 g         3628 t
ORIGIN      
        1 ccacgcttat tttttgacat cctgctagcc ccatctacgc tctaatagtg gaagcggagg
       61 gactataaaa tgaaaggtga tgttaaaatc ataaattatc tcaataaact attgggaaat
      121 gagcttgtcg caatcaatca gtattttctc catgcccgga tgtttaaaaa ctggggcctg
      181 atgcgtctca atgacgtcga ataccatgaa tccatcgatg agatgaaaca cgctgataaa
      241 tatatcgagc gtattttgtt tttagaagga atccccaact tacaggattt aggcaaactg
      301 gggattggtg aagatgtcga ggagatgctg caatctgacc tgcgattaga actggaaggc
      361 gcgaaggatc tgcgtgaggc tatcgcatat gccgacagcg ttcatgacta tgttagccgc
      421 gatatgatga tcgagatcct tgctgatgaa gaaggtcata tcgactggct cgaaactgaa
      481 ctggatctca tcggtaaaat cggcctacaa aattacctgc aatcacaaat taaagttaaa
      541 gattaacggc ctatcagcaa actatcccag cctataacga aacccgcagc cgccagaaat
      601 ggcccaaaag gcagcgggtt ttttaatgat tcttttcctc tcatgagcag accaataact
      661 acggctccgc aagcgaatga ggcagcaagg aaaaccagtc gtggtaagaa ggcccatgaa
      721 tgctaggcgc caagggcggc gagaaacttc acatcaccat agcctaatcc ttctttatgt
      781 cgcagtatgc gataccccca gtaaatgaca gcgaatgtgc cgtaaccaac aattgccccc
      841 cataacgcat cggctaaacc atcaggatga caaacttgat agaacaaaag acctgaccag
      901 agtaacgggc acgtaaatct gtcaggtaac aagccatgct ttgcatccca gaaaaaaagt
      961 agggctgata aacaagcgta caaaattaga aagggaaggg tagtgtccat aaaacatcct
     1021 ctgtaattgg agtatcacca tcgaataact ccggatgctc agcaacagag agagcgctga
     1081 tttgttaacc tgattccggt gaaaaacgct atcttcacaa ccttacaaat ttcgtggaat
     1141 gcagctcgca atctgcgcgt tgcgattaat ttctacccga tgcttgcgtc aggctcagat
     1201 ttgcgtataa tgcgcgggct tgtcgtagtt gacagcaggt tcaatctaaa cctcagttag
     1261 ccgaatttgg ctacctaaca atgctcccaa tcggggagct acgtaagaac ggttacactc
     1321 tcccatcaat cgtaatgggt ctgaggagta atcattttcg tttataaaat aattggagct
     1381 ctggtctcat gcagaaccaa agaatccgta tccgcctgaa agcgtttgat catcgtctga
     1441 tcgatcaagc aaccgcggaa atcgtcgaga ctgccaagcg cactggtgcg caggtccgtg
     1501 gtccgatccc gctgccgaca cgcaaagagc gcttcactgt tctgatctcc ccgcacgtca
     1561 acaaagacgc gcgcgatcag tacgaaatcc gtactcactt gcgtctggtt gacatcgttg
     1621 agccaaccga gaaaaccgtt gatgctctga tgcgtctgga tctggctgcc ggtgtagacg
     1681 tgcagatcag cctgggttaa tcaggtcatt gagcgattga gaggttgaaa caatgattgg
     1741 tttagtcggt aaaaaagtgg gtatgacccg tatcttcaca gaagacggcg tttctatccc
     1801 agtaaccgta atcgaagttg aagcaaaccg cgttactcag gttaaagacc tggctaacga
     1861 tggctaccgt gctattcagg tgaccaccgg tgctaaaaaa gctaaccgtg tgaccaagcc
     1921 tgaagctggc cacttcgcta aagctggcgt agaagctggc cgtggtctgt gggaattccg
     1981 cctggctgaa ggcgaagagt tcactgtagg tcagagcatt agcgttgaac tgtttgctga
     2041 cgttaaaaaa gttgacgtaa ctggcacctc taaaggtaaa ggtttcgcag gtaccgttaa
     2101 gcgctggaac ttccgtaccc aggacgctac tcacggtaac tccttgtctc accgcgttcc
     2161 gggttctatc ggtcagaacc agactccggg caaagtgttc aaaggcaaga aaatggcagg
     2221 tcagatgggt aacgaacgtg taaccgttca gagccttgac gtagtacgcg ttgacgctga
     2281 gcgcaacctg ctgctggtta aaggtgctgt cccgggtgca accggtagcg acctgatcgt
     2341 taaaccagct gtgaaggcgt aaggagatag caatggaatt agtattgaaa gacgcgcaga
     2401 gcgcgctgac tgtttccgaa actaccttcg gtcgtgattt caacgaagcg ctggttcacc
     2461 aggttgttgt tgcttatgca gctggtgctc gtcagggtac tcgtgctcag aagactcgtg
     2521 ctgaagtaac tggttccggt aaaaaaccgt ggcgccagaa aggcaccggc cgtgcgcgtt
     2581 ctggttctat caagagcccg atctggcgtt ctggtggcgt gacctttgct gctcgtccgc
     2641 aggaccacag tcaaaaagtt aacaagaaga tgtaccgcgg cgcgctgaaa agcatcctgt
     2701 ccgaactggt acgtcaggat cgtctgatcg ttgtcgagaa gttctctgta gaagcgccga
     2761 aaactaagct gctggcacag aaactgaaag acatggctct ggaagatgtg ctgatcatca
     2821 ccggtgagct ggacgaaaac ctgttcctgg ctgcgcgcaa cctgcagaag gttgacgtac
     2881 gcgatgcaac tggtatcgac ccggttagcc tgatcgcctt cgacaaagtc gtaatgactg
     2941 ctgatgctgt taagcaagtt gaggagatgc tggcatgatt cgtgaagaac gtctgctgaa
     3001 ggtgctgcgt gcaccgcacg tttctgaaaa agcgtctact gcgatggaaa aatccaacac
     3061 catcgtactc aaagttgcta aagacgcgac caaagcagaa atcaaagctg ctgtgcagaa
     3121 actgtttgaa gtcgaagtcg aagtcgttaa caccctggta gttaaaggga aagttaaacg
     3181 tcacggacag cgtatcggtc gtcgtagcga ctggaaaaaa gcttacgtca ccctgaaaga
     3241 aggccagaat ctggacttcg ttggcggcgc tgagtaagtc ggaggagtaa tacaatggca
     3301 gttgttaaat gtaaaccgac atctccgggt cgtcgccacg tagttaaagt ggttaaccct
     3361 gagctgcaca agggcaaacc ttttgctccg ttgctggaaa aaaacagcaa atccggtggt
     3421 cgtaacaaca atggccgtat caccactcgt catatcggtg gtggccacaa gcaggcttac
     3481 cgtattgttg acttcaaacg caacaaagac ggtatcccgg cagttgttga acgtcttgag
     3541 tacgatccga accgttccgc gaacatcgcg ctggttctgt acaaagacgg tgaacgccgt
     3601 tacatcctgg cccctaaagg cctgaaagct ggcgaccaga ttcagtctgg cgttgatgct
     3661 gcaatcaaac caggtaacac cctgccgatg cgcaacatcc cggttggttc tactgttcat
     3721 aacgtagaaa tgaaaccagg taaaggcggt cagctggcac gttccgctgg tacttacgtt
     3781 cagatcgttg ctcgtgatgg tgcttatgtc accctgcgtc tgcgttctgg tgaaatgcgt
     3841 aaagtagaag cagactgccg tgcaactctg ggcgaagttg gcaatgctga gcatatgctg
     3901 cgcgttctgg gtaaagcagg tgctgcacgc tggcgtggtg ttcgtccgac cgttcgcggt
     3961 accgcgatga acccggtaga ccacccacat ggtggtggtg aaggtcgtaa ctttggtaag
     4021 cacccggtaa ctccgtgggg cgttcagacc aaaggtaaga agacccgcag caacaagcgt
     4081 actgataaat tcatcgtacg tcgccgtagc aaataatttt agaggataag ccatgccacg
     4141 ttctctcaag aaaggtcctt ttattgacct gcacttgctg aagaaggtag agaaagcggt
     4201 ggaaagcgga gacaagaagc ccctgcgcac ttggtcccgt cgttcaacga tctttcctaa
     4261 catgatcggt ttgaccatcg ctgtccataa tggtcgtcag cacgttccgg tatttgtaac
     4321 cgacgaaatg gtcggtcaca aactgggtga attcgcaccg actcgtactt atcgcggcca
     4381 cgctgctgat aaaaaagcga agaagaaata aggtaggagg aagagatgga aactattgct
     4441 aaacatcgcc atgctcgttc ttctgctcag aaggttcgcc ttgttgctga cctgattcgc
     4501 ggtaagaaag tgtcgcaggc tctggatatt ttgacctaca ccaacaagaa agcggctgta
     4561 caggtcaaga aagttctgga atctgccatt gctaacgctg aacacaacga tggcgctgac
     4621 attgacgatc tgaaagttac gaaaattttc gtagacgaag gcccgagcat gaagcgcatt
     4681 atgccgcgtg caaaaggtcg tgcagatcgc atcctgaagc gcaccagcca catcactgtg
     4741 gttgtgtccg atcgctgaga ctctggagac tagcaatggg tcagaaagta catcctaatg
     4801 gtattcgctt gggtattgta aaaccatgga actctacttg gtttgcgaac accaaagaat
     4861 tcgctgacaa cctggacagc gattttaaag tacgtcagta cctgactaag gaactggcta
     4921 aagcgtccgt atctcgtatc gttatcgagc gtccggctaa gagcatccgt gtaaccattc
     4981 acactgctcg cccgggtatc gttatcggta aaaaaggtga agacgtagaa aaactgcgta
     5041 aggtcgtagc ggacatcgct ggcgttcctg cacagatcaa catcgccgaa gttcgtaagc
     5101 ctgaactgga cgcaaaactg gttgctgaca gcatcacttc tcagctggaa cgtcgcgtta
     5161 tgttccgtcg tgctatgaag cgtgctgtac agaacgcaat gcgtctgggc gctaaaggta
     5221 ttaaagttga agttagcggc cgtctgggcg gcgcggaaat cgcacgtacc gaatggtacc
     5281 gcgaaggtcg cgtaccgctg cacactctgc gtgctgacat cgactacaac acctctgaag
     5341 cgcacaccac ttacggtgta atcggcgtta aagtgtggat cttcaaaggc gagatcctgg
     5401 gtggtatggc tgctgttgaa caaccggaaa aaccggctgc tcagcctaaa aagcagcagc
     5461 gtaaaggccg taaataagga gcgtcgctga tgttacaacc aaagcgtaca aaattccgta
     5521 aaatgcacaa aggccgtaac cgcggtctgg cgcagggtac ggatgttagc ttcggcagct
     5581 tcggtctgaa agctgttggc cgtggtcgtc tgactgcccg tcagatcgaa gcagcacgtc
     5641 gtgctatgac ccgtgcagtt aagcgtcaag gtaagatctg gatccgtgtg ttcccggaca
     5701 aaccgatcac tgaaaagccg ctggcagtgc gtatgggtaa aggtaaaggt aacgtggagt
     5761 attgggttgc cttgattcag ccgggtaaag tcctgtatga aatggacggt gttccggaag
     5821 agctggcccg tgaagcattc aagctggcag cagcgaaact gccgattaaa accacctttg
     5881 taactaagac ggtgatgtaa tgaaagcaaa agagctgcgt gagaagagca ttgaagagct
     5941 gaacaccgag ctgctgaacc tgctgcgtga gcagttcaac ctgcgtatgc aggctgcaag
     6001 tggccagctg caacagtctc acctgttgaa gcaagtgcgt cgcgatgtcg cacgcgttaa
     6061 gactttactg aacgagaagg cgggtgcgta atgaccgata aaatccgtac tctgcaaggt
     6121 cgcgttgtta gcgacaaaat ggagaaatcc attgttgttg ctatcgaacg ttttgtgaaa
     6181 cacccgatct acggaaaatt catcaagcgt acgaccaaac tgcacgtaca tgacgagaac
     6241 aacgaatgcg gtatcggtga cgtggttgaa atccgcgaat gccgtccgct gtccaagact
     6301 aaatcctgga cgctggttcg cgttgtagag aaagcggttc tgtaatacag tacactctct
     6361 cgatacgaat aaacggctca gaaatgagcc gtttattttt tctacccata tccttgaagc
     6421 ggtgttataa tgccgcgccc tcgatatggg gatttttaac gacctgattt tcgggtctca
     6481 gtagtagttg acattagcgg agcactaaaa tgatccaaga acagactatg ctgaacgtcg
     6541 ccgacaactc cggtgcacgt cgcgtaatgt gtatcaaggt tctgggtggc tcgcaccgtc
     6601 gctacgcagg cgtaggcgac atcatcaaga tcaccatcaa agaagcaatt ccgcgtggta
     6661 aggtcaaaaa aggtgatgtg ctgaaggcgg tagtggtgcg caccaagaag ggtgttcgtc
     6721 gcccggacgg ttctgtcatt cgcttcgatg gtaatgcttg tgttcttctg aacaacaaca
     6781 gcgagcagcc tatcggtacg cgtatttttg ggccggtaac tcgtgagctt cgtagtgaga
     6841 agttcatgaa aattatctct ctggcaccag aagtactcta aggagcgaat catggcagcg
     6901 aaaatccgtc gtgatgacga agttatcgtg ttaaccggta aagataaagg taaacgcggt
     6961 aaagttaaga atgtcctgtc ttccggcaag gtcattattg aaggtatcaa cctggttaag
     7021 aaacatcaga agccggttcc ggccctgaac caaccgggtg gcatcgttga aaaagaagcc
     7081 gctattcagg tttccaacgt agcaatcttc aatgcggcaa ccggcaaggc tgaccgtgta
     7141 ggctttagat tcgaagacgg caaaaaagtc cgtttcttca agtctaacag cgaaactatc
     7201 aagtaatttg gagtagtacg atggcgaaac tgcatgatta ctacaaagac gaagtagtta
     7261 aaaaactcat gactgagttt aactacaatt ctgtcatgca agtccctcgg gtcgagaaga
     7321 tcaccctgaa catgggtgtt ggtgaagcga tcgctgacaa aaaactgctg gataacgcag
     7381 cagcagacct ggcagcaatc tccggtcaaa aaccgctgat caccaaagca cgcaaatctg
     7441 ttgcaggctt caaaatccgt cagggctatc cgatcggctg taaagtaact ctgcgtggcg
     7501 aacgcatgtg ggagttcttt gagcgcctga tcactattgc tgtacctcgt atccgtgact
     7561 tccgtggcct gtccgctaag tctttcgacg gtcgtggtaa ctacagcatg ggtgtccgtg
     7621 agcagatcat cttcccagaa atcgactacg ataaagtcga ccgcgttcgt ggtttggata
     7681 ttaccattac cactactgcg aaatctgacg aagaaggccg cgctctgctg gctgcctttg
     7741 acttcccgtt ccgcaagtaa ggtagggtta ctaaatggct aagcaatcaa tgaaagcacg
     7801 cgaagtaaaa cgcgtagctt tagctgataa atacttcgcg aaacgcgctg aactgaaagc
     7861 gatcatctct gatgtgaacg cttccgacga agatcgttgg aacgctgttc tcaagctgca
     7921 gactctgccg cgtgattcca gcccgtctcg tcagcgtaac cgctgccgtc aaacaggtcg
     7981 tccgcatggt ttcctgcgga agttcgggtt gagccgtatt aaggtccgtg aagccgctat
     8041 gcgcggtgaa atcccgggtc tgaaaaaggc tagctggtaa ttgtcaccaa ttgaatcacg
     8101 ggagtaaaga cagatgagca tgcaagatcc gatcgcggat atgctgaccc gtatccgtaa
     8161 cggtcaggcc gcgaacaaag ctgcggtcac catgccttcc tccaagctga aagtggcaat
     8221 cgccaacgtg ctgaaggaag aaggttttat tgaagatttt aaagttgaag gcgacaccaa
     8281 gcctgaactg gaacttactc tgaagtattt ccagggcaaa gctgttgtag aaagcattca
     8341 gcgtgtcagc cgcccaggtc tgcgcatcta taaacgtaaa gatgagctgc cgaaagttat
     8401 ggcgggtctg ggtatcgcag ttgtttctac ctctaaaggt gttatgactg atcgtgcagc
     8461 gcgccaggct ggtcttggtg gcgaaattat ctgctacgta gcctaatcgg aggaaaaaat
     8521 gtctcgtgtt gctaaagcac cggtcgttgt tcctgccggc gttgacgtaa aaatcaacgg
     8581 tcaggttatt acgatcaaag gtaaaaacgg cgagctgact cgtactctca acgatgctgt
     8641 tgaagttaaa catgcagata ataccctgac cttcggtccg cgtgatggtt acgcagacgg
     8701 ttgggcacag gctggtaccg cgcgtgccct gctgaactca atggttatcg gtgttaccga
     8761 aggcttcact aagaagctgc agctggttgg tgtaggttac cgtgcagcgg ttaaaggcaa
     8821 tgtgattaac ctgtctctgg gtttctctca tcctgttgac catcagctgc ctgcgggtat
     8881 cactgctgaa tgtccgactc agactgaaat cgtgctgaaa ggcgctgata agcaggtgat
     8941 cggccaggtt gcagcggatc tgcgcgccta ccgtcgtcct gagccttaca aaggcaaggg
     9001 ggttcgttac gccgacgaag tcgtgcgtac caaagaggct aagaagaagt aaggtaacac
     9061 tatggataag aaatctgctc gtatccgtcg tgcgacccgc gcacgccgca agctccagga
     9121 gctgggcgca actcgcctgg ttgtacatcg taccccgcgt cacatttacg cacaggtaat
     9181 tgcaccgaac ggttctgaag ttctggtagc tgcttctact gtagaaaaag ctatcgctga
     9241 acaactgaag tacaccggta acaaagacgc ggctgcagct gtgggtaaag ctgtcgctga
     9301 acgcgctctg gaaaaaggca tcaaagatgt atcctttgac cgttccgggt tccaatatca
     9361 tggtcgtgtc caggcactgg cagatgctgc ccgtgaagct ggccttcagt tctaaggtag
     9421 aggtgtaaga tggctcacat cgaaaaacaa gctggcgaac tgcaggaaaa gctgatcgcg
     9481 gtaaaccgcg tatctaaaac cgttaaaggt ggtcgtattt tctccttcac agctctgact
     9541 gtagttggcg atggtaacgg tcgcgttggt tttggttacg gtaaagcgcg tgaagttcca
     9601 gcagcgatcc agaaagcgat ggaaaaagcc cgtcgcaata tgattaacgt cgcgctgaat
     9661 aacggcactc tacaacaccc tgttaaaggt gttcacacgg gttctcgcgt attcatgcag
     9721 ccggcttccg aaggtaccgg tatcatcgcc ggtggtgcaa tgcgcgccgt tctggaagtc
     9781 gctggggttc ataacgttct ggctaaagca tatggttcca ccaacccgat caacgtggtt
     9841 cgtgcaacta ttgatggcct ggaaaatatg aattctccag aaatggtcgc tgccaagcgt
     9901 ggtaaatccg ttgaagaaat tctggggaaa taaaccatgg caaagactat taaaattact
     9961 caaacccgca gtgcaatcgg tcgtctgccg aaacacaagg caacgctgct tggcttgggt
    10021 ctgcgtcgta ttggtcacac cgtagagcgc gaggatactc ctgctattcg cggtatgatc
    10081 aacgcggttt ccttcatggt taaagttgag gagtaagaga tgcgtttaaa tactctgtct
    10141 ccggccgaag gctccaaaaa ggcgggtaaa cgcctgggtc gtggtatcgg ttctggcctc
    10201 ggtaaaaccg gtggtcgtgg tcacaaaggt cagaagtctc gttctggcgg tggcgtacgt
    10261 cgcggtttcg agggtggtca gatgcctctg taccgtcgtc tgccgaaatt cggcttcact
    10321 tctcgtaaag cagcgattac agccgaagtt cgtctatctg acctggctaa agtagaaggc
    10381 ggtgttgtag acctgaacac gctgaaagcg gctaacatta tcggtatcca gatcgagttc
    10441 gcgaaagtga tcctggctgg cgaagtaacg actccggtaa ctgttcgtgg cctgcgtgtt
    10501 actaaaggcg ctcgtgctgc tatcgaagct gctggcggta aaatcgagga ataagtagca
    10561 gatggctaaa caaccgggat tagattttca aagtgccaaa ggtggcttag gcgagctgaa
    10621 acgcagactg ctgtttgtta tcggtgcgct gattgtgttc cgtattggct cttttattcc
    10681 gatccctggt attgatgccg ctgtacttgc caaactgctt gagcaacagc gaggcaccat
    10741 cattgagatg tttaacatgt tctctggtgg tgctctcagc cgtgcttcta tctttgctct
    10801 ggggatcatg ccgtatattt cggcgtcgat cattatccag ctgctgacgg tggttcaccc
    10861 aacgttggca gaaattaaga aagaagggga gtctggtcgt cgtaagatca gccagtacac
    10921 ccgttacggt actctggtgc tggcaatatt ccagtcgatc ggtattgcta ccggtctgcc
    10981 gaatatgcct ggtatgcaag gcctggtgat taacccgggc tttgcattct acttcaccgc
    11041 tgttgtaagt ctggtcacag gaacgatgtt cctgatgtgg ttgggcgaac agatcactga
    11101 acgaggtatc ggcaacggta tttcaatcat tatcttcgcc ggtattgtcg cgggactccc
    11161 gccagccatt gcccatacta tcgagcaagc gcgtcaaggc gacctgcact tcctcgtgtt
    11221 gctgttggtt gcagtattag tatttgcagt gacgttcttt gttgtgtttg ttgagcgtgg
    11281 tcaacgccgc attgtggtaa actacgcgaa acgtcagcaa ggtcgtcgtg tctatgctgc
    11341 acagagcaca catttaccgc tgaaagtgaa tatggcgggg gtaatcccgg caatcttcgc
    11401 ttccagtatt attctgttcc cggcgaccat cgcgtcatgg ttcgggggcg gtactggttg
    11461 gaactggctg acaacaattt cgctgtattt gcagcctggg caaccgcttt atgtgttact
    11521 ctatgcgtct gcaatcatct tcttctgttt cttctacacg gcgttggttt tcaacccgcg
    11581 tgaaacagca gataacctga agaagtccgg tgcatttgta ccaggaattc gtccgggaga
    11641 gcaaacggcg aagtatatcg ataaagtaat gacccgcctg accttggttg gtgcgctgta
    11701 tattaccttt atctgcctga tcccggagtt catgcgtgat gcaatgaaag taccgttcta
    11761 cttcggtggg acctcactgc ttatcgttgt tgtcgtgatt atggacttta tggctcaagt
    11821 gcaaactctg atgatgtcca gtcagtatga gtctgcattg aagaaggcga acctgaaagg
    11881 ctacggccga taattggtcg cccgagaagt tacggagagt aaaaatgaaa gttcgtgctt
    11941 ccgtcaagaa attatgccgt aactgcaaaa tcgttaagcg tgatggtgtc atccgtgtga
    12001 tttgcagtgc cgagccgaag cataaacagc gccaaggctg attttttcgc atatttttct
    12061 tgcaaagttg ggttgagctg gctagattag ccagccaatc ttttgtatgt ctgtacgttt
    12121 ccatttgagt atcctgaaaa cgggcttttc agcatggaac gtacatatta aatagtagga
    12181 gtgcatagtg gcccgtatag caggcattaa cattcctgat cataagcatg ccgtaatcgc
    12241 attaacttcg atttatggcg tcggcaagac ccgttctaaa gccatcctgg ctgcagcggg
    12301 tatcgctgaa gatgttaaga tcagtgagct gtctgaagga caaatcgaca cgctgcgtga
    12361 cgaagttgcc aaatttgtcg ttgaaggtga tctgcgccgt gaaatcagca tgagcatcaa
    12421 gcgcctgatg gatcttggtt gctatcgcgg tttgcgtcat cgtcgtggtc tcccggttcg
    12481 cggtcagcgt accaagacca acgcacgtac ccgtaagggt ccgcgcaaac cgatcaagaa
    12541 ataatcgggg tgattgaata atggcaaagg caccaattcg tgcacgtaaa cgtgtaagaa
    12601 aacaagtctc tgacggcgtg gctcatatcc atgcttcttt caacaacacc atcgtgacta
    12661 tcactgatcg tcagggtaac gcgttgggtt gggcaacagc cggtggttcc ggtttccgtg
    12721 gttctcgcaa atccactccg tttgcagctc aggttgcagc agagcgttgc gctgacgccg
    12781 tgaaagaata cggcatcaag aatctggaag ttatggttaa aggtccgggt ccaggccgcg
    12841 aatctactat tcgtgttctg aacgccgcag gtttccgcat cactaacatt actgatgtga
    12901 ctccgatccc tcataacggt tgtcgtccgc cgaaaaaacg tcgcgtataa cgcctcgttt
    12961 tccaggtttg ttggagaaag aaaatggcaa gatatttggg tcctaagctc aagctgagcc
    13021 gtcgtgaggg caccgactta ttccttaagt ctggcgttcg cgcgatcgat accaagtgta
    13081 aaattgaaca agctcctggc cagcacggtg cgcgtaagcc gcgtctgtct gactatggtg
    13141 tgcagttgcg tgaaaagcaa aaagttcgcc gtatctatgg tgtgctggag cgtcagttcc
    13201 gtaactacta caaagaagca gcacgtctga aaggcaacac cggtgaaaac ctgttggctc
    13261 tgctggaagg tcgtctggac aacgttgtat accgtatggg cttcggtgcc actcgtgcag
    13321 aagcacgtca gctggttagc cataaagcaa ttatggtaaa cggtcgtgtt gttaacatcg
    13381 cttcttatca ggttagtccg aatgacgttg taagcattcg tgagaaagcg aagaagcagt
    13441 ctcgcgtgaa agccgctctg gagctggctg agcagcgtga aaagccaact tggctggaag
    13501 ttgatgctgg caagatggaa ggtacgttta agcgtaagcc ggagcgttct gatctgtctg
    13561 cggacattaa cgaacacctg atcgtcgagc tttactccaa gtaaagctta gtaccaaaga
    13621 gaggacacaa tgcagggttc tgtgacagag tttctaaaac cgcgcctggt tgatatcgag
    13681 caagtgagtt cgacgcacgc caaggtgacc cttgagcctt tagagcgtgg ctttggccat
    13741 actctgggta acgcactgcg ccgtattctg ctctcatcga tgccgggttg cgcggtgacc
    13801 gaggttgaga ttgatggtgt actacatgag tacagcacca aagaaggcgt tcaggaagat
    13861 atcctggaaa tcctgctcaa cctgaaaggg ctggcggtga gagttcaggg caaagatgaa
    13921 gttattctta ccttgaataa atctggcatt ggccctgtga ctgcagccga tatcacccac
    13981 gacggtgatg ttgaaatcgt caagccgcag cacgtgatct gccacctgac cgatgagaac
    14041 gcgtctatta gcatgcgtat caaagttcag cgcggtcgtg gttatgtgcc ggcttctacc
    14101 cgaattcatt cggaagaaga tgagcgccca atcggccgtc tgctggtcga cgcatgctac
    14161 agccctgtgg agcgtattgc ctacaatgtt gaagcagcgc gtgtagaaca gcgtaccgac
    14221 ctggacaagc tggtcatcga aatggaaacc aacggcacaa tcgatcctga agaggcgatt
    14281 cgtcgtgcgg caaccattct ggctgaacaa ctggaagctt tcgttgactt acgtgatgta
    14341 cgtcagcctg aagtgaaaga agagaaacca gagttcgatc cgatcctgct gcgccctgtt
    14401 gacgatctgg aattgactgt ccgctctgct aactgcctta aagcagaagc tatccactat
    14461 atcggtgatc tggtacagcg taccgaggtt gagctcctta aaacgcctaa ccttggtaaa
    14521 aaatctctta ctgagattaa agacgtgctg gcttcccgtg gactgtctct gggcatgcgc
    14581 ttggaaaact ggccaccggc aagcatcgct gacgagtaac cggatcacag gttaaggttt
    14641 tactgagaag gataaggtca tgcgccatcg taagagtggt cgtcaactga accgcaacag
    14701 cagccatcgc caggctatgt tccgcaatat ggcaggttca ctggttcgtc atgaaatcat
    14761 caagacgact ctgcctaaag cgaaagagct gcgccgcgta gttgagccgc tgattactct
    14821 tgccaagact gatagcgttg ctaatcgtcg tctggcattc gcccgtactc gtgataacga
    14881 gatcgtggca aaactgttta acgaactggg cccgcgtttc gcgagccgtg ccggtggtta
    14941 cactcgtatt ctgaagtgtg gcttccgtgc aggcgacaac gcgccgatgg cttacatcga
    15001 gctggttgat cgttcagaga aagcagaagc tgctgcagag taatctgaag caatgtaaaa
    15061 aaacccgccc cggcgggttt ttttataccc gtagtatccc cacttatcta caatagctgt
    15121 actcttttt
//
LOCUS       DAZSNM010000092        15101 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZSNM010000092 DAZSNM010000000
VERSION     DAZSNM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15101
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.092"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..956)
                     /locus_tag="ACHW42_002343"
     CDS             complement(<1..956)
                     /locus_tag="ACHW42_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFR8944144.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="ACHW42_002344"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="ACHW42_002344"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFR8944145.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHW42_002345"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHW42_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHW42_002346"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHW42_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR8944146.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="ACHW42_002347"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="ACHW42_002347"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR8944147.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="ACHW42_002348"
     CDS             complement(5018..6346)
                     /locus_tag="ACHW42_002348"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR8944148.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8696)
                     /locus_tag="ACHW42_002349"
                     /pseudo
     CDS             complement(6354..8696)
                     /locus_tag="ACHW42_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="ACHW42_002350"
     CDS             complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="ACHW42_002350"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR8944149.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="ACHW42_002351"
     CDS             complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="ACHW42_002351"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR8944150.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="ACHW42_002352"
     CDS             complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="ACHW42_002352"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR8944151.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="ACHW42_002353"
     CDS             complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="ACHW42_002353"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR8944152.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14142..>15101)
                     /gene="iaaA"
                     /locus_tag="ACHW42_002354"
     CDS             complement(14142..>15101)
                     /gene="iaaA"
                     /locus_tag="ACHW42_002354"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR8944153.1"
                     /translation="KAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLEAG
                     ESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSHLR
                     NPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEGAT
                     VLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYA
                     NNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLI
                     AIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
BASE COUNT         3926 a         4044 c         3448 g         3683 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatctttt cgtataaact cagcatgtta
     8701 atacatttaa caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc
     8761 ggctttttaa ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta
     8821 gggcaataat ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct
     8881 ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg
     8941 gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct
     9001 cgtgcctcat tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga
     9061 aatgacaggc tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg
     9121 atagaagaga cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca
     9181 atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc
     9241 aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg
     9301 cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca
     9361 tcgcaaatgc gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc
     9421 agcaacgtcc ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt
     9481 tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag
     9541 tgctgcaaag aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag
     9601 ggagcgatcc agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg
     9661 gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc
     9721 tggtcaggtt tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt
     9781 cgcatcctta cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta
     9841 agttgataag aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat
     9901 cgcgcatttc tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct
     9961 ccaccacgat agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg
    10021 ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg
    10081 ccctcgcggt acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc
    10141 gcgccatcac ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc
    10201 aactgtctgc tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa
    10261 acatccccag cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc
    10321 gatccggcca acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca
    10381 ccgcccagac catactggtt atggtcagcc atagcgttgg cataaagcgg ctggcaatct
    10441 catcggcaac cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc
    10501 tgatatagtg ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca
    10561 gttctataac ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca
    10621 gcatgtggac aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca
    10681 gtaatcgttt gataacgtaa taaagcattc cactccctgc gttgcttatt gcaaatccgc
    10741 gtcttcaaag ctgaagccgg tatctggcat gatccaaaaa ccggtcaggt ttttactgtg
    10801 tgccgacacc agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc
    10861 ctgcgccgcc ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc
    10921 caggaagtca tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca
    10981 gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca
    11041 gccagtgtag aacatccgca cgccactctc tttttgccct ttaccttcaa cttctgccgc
    11101 ccgctgtccg gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg
    11161 ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt
    11221 actgaaaccg ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta
    11281 aggccacggt ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg
    11341 agttgcatag cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag
    11401 cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg
    11461 ctgcataatt gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc
    11521 ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg
    11581 ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg
    11641 ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc
    11701 atacggtccg gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt
    11761 cggtgaaatc atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg
    11821 tttgagggta atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata
    11881 caggttatag cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac
    11941 cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt
    12001 gtaagtaatg ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat
    12061 ctctttatcc agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt
    12121 gtcattcgcg tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc
    12181 tttggcagcg aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc
    12241 actacggtgt acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg
    12301 cgtattctga ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg
    12361 ctgccacctc ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac
    12421 gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat
    12481 aaggatgctg cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga
    12541 gatacatcac cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa
    12601 acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc
    12661 gaatagaaac atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg
    12721 ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac
    12781 gccaggcatg ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg
    12841 cgtctttacc tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg
    12901 tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca
    12961 atgcctgaag tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc
    13021 cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag
    13081 actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg
    13141 catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa
    13201 gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt
    13261 cctgtttcgc tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt
    13321 tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg
    13381 catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta
    13441 cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg
    13501 acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca
    13561 gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa
    13621 tcatcactcg ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt
    13681 gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac
    13741 gactggcgtt ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg
    13801 ggttcagcga tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc
    13861 gcatttgtgc agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca
    13921 gcattttatc gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg
    13981 cagtcactga cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca
    14041 gactaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat
    14101 taagattttc aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg
    14161 tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga
    14221 tacattcctt cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag
    14281 ccaccgctac cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg
    14341 agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg
    14401 aagacttcgc ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg
    14461 gcacccacta agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg
    14521 gacgtggctg ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt
    14581 ttctgttttt catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc
    14641 gctgccagta gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc
    14701 atgccatgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt
    14761 tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc
    14821 gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca
    14881 cgcgtaaaga cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc
    14941 gcttccgtca ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca
    15001 acaatggcag acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg
    15061 ctaattgcac ctgcgccacc atgaattgca atgactgctt t
//
LOCUS       DAZSNM010000093        14764 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZSNM010000093 DAZSNM010000000
VERSION     DAZSNM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14764
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.093"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..276
                     /locus_tag="ACHW42_002355"
     CDS             <1..276
                     /locus_tag="ACHW42_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8944154.1"
                     /translation="ERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKR
                     YTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(306..1247)
                     /gene="fabY"
                     /locus_tag="ACHW42_002356"
                     /gene_synonym="yiiD"
     CDS             complement(306..1247)
                     /gene="fabY"
                     /locus_tag="ACHW42_002356"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR8944155.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1292..1729)
                     /gene="dtd"
                     /locus_tag="ACHW42_002357"
     CDS             complement(1292..1729)
                     /gene="dtd"
                     /locus_tag="ACHW42_002357"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR8944156.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1726..2598)
                     /locus_tag="ACHW42_002358"
     CDS             complement(1726..2598)
                     /locus_tag="ACHW42_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR8944157.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2592..3191)
                     /gene="yihX"
                     /locus_tag="ACHW42_002359"
     CDS             complement(2592..3191)
                     /gene="yihX"
                     /locus_tag="ACHW42_002359"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR8944158.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3290..4075)
                     /locus_tag="ACHW42_002360"
     CDS             complement(3290..4075)
                     /locus_tag="ACHW42_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR8944159.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4109..5005)
                     /gene="yihV"
                     /locus_tag="ACHW42_002361"
     CDS             complement(4109..5005)
                     /gene="yihV"
                     /locus_tag="ACHW42_002361"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFR8944160.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5173..6069
                     /gene="yihU"
                     /locus_tag="ACHW42_002362"
     CDS             5173..6069
                     /gene="yihU"
                     /locus_tag="ACHW42_002362"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR8944161.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6093..6971
                     /gene="yihT"
                     /locus_tag="ACHW42_002363"
     CDS             6093..6971
                     /gene="yihT"
                     /locus_tag="ACHW42_002363"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFR8944162.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6988..8229
                     /gene="yihS"
                     /locus_tag="ACHW42_002364"
     CDS             6988..8229
                     /gene="yihS"
                     /locus_tag="ACHW42_002364"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFR8944163.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8367..9269
                     /locus_tag="ACHW42_002365"
     CDS             8367..9269
                     /locus_tag="ACHW42_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFR8944164.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9468..11504
                     /gene="yihQ"
                     /locus_tag="ACHW42_002366"
     CDS             9468..11504
                     /gene="yihQ"
                     /locus_tag="ACHW42_002366"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFR8944165.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11550..12924
                     /locus_tag="ACHW42_002367"
                     /pseudo
     CDS             11550..12924
                     /locus_tag="ACHW42_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12967..14370
                     /locus_tag="ACHW42_002368"
     CDS             12967..14370
                     /locus_tag="ACHW42_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8944166.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14438..>14764
                     /locus_tag="ACHW42_002369"
     CDS             14438..>14764
                     /locus_tag="ACHW42_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFR8944167.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3566 a         3841 c         3729 g         3628 t
ORIGIN      
        1 gaacgcactc tggccacact ggagcgtctt ctgagcctgc tgtcggcctt tgaggtcgtg
       61 gtatggatga cggatggctg gccgctgtat gaatcacgcc tgaagggaaa gctgcacgtt
      121 atcagcaagc gttacactca gcgcattgag cgacataatc tgaatctgag acaacatctg
      181 gcaaggctgg gacggaagtc actgtcgttc tcaaaatcgg tggagctgca tgacaaggtc
      241 atcgggcatt atctgaacat aaaacactat cagtaagttg gagtcattac ccccctctcc
      301 ataagctact cttcttcgtt cccgccctct tcatacgggc caaatggctt cgcgggcaga
      361 acgatatacg tgccttcaaa cactgcaccc ggcgtctcgt cgccaaagat ttcgacctgc
      421 atctgcaccc gtgcttttcg tccgcgcgcc agacggtcga gatcgccgct taaggcacca
      481 aggtcggcta ccgcatgggg tttaccgcta atcggtttgc tgtagcggat atgcgcatcc
      541 gccagaataa tcgttccgcc gaggtggcgt tcacgcagca tcagccagat aagcccccaa
      601 ccggtgagcg tcgccagcga gaataaactc ccggcaaaca gcgtatggtg cggattctga
      661 ttgccggttt ccggcatggt ggtgataaat ttttgcccgg tatattgttg aatacgcacg
      721 cccatttttt cactaagcgg gatatgttcg taccacgcct gttgcagctg cgcgcaccag
      781 tcgccgcgat gcaaaatatc atccagagtg gcgacgggct taatcatcaa aaaatggcga
      841 atcggcgtgg tggttggcgt ggtgatttct ccctgattaa caaaccccag cttggcgaaa
      901 aactccaccg cgtcttcacg ggcgctacag gtcacgcgct taacgccttc ctgacgcgcc
      961 accgactcca gggtcatcgc catcagcgtt cctaaccctt tgtcctgcac gtccgggtga
     1021 acgaccataa agcgaatgga cgcttcattg tcggcattaa tatacaatcg gcctaccgcc
     1081 accagattac cctgctcgtc gacgaccatc tggtgatgcg ccatcgcatc ccacgcgtcg
     1141 cgttccgaac cttttggttg atgcaggggc ttacgcaaca tttcccagcg aaagtgatag
     1201 taacgctcta actcttcttc tgtttgtgga acctgaaggt gatacatagc gatactctct
     1261 cttgtttccc gtgacaaccc tggaagctgg ctcatacctg caaccagaat gtcacggggc
     1321 catcattgac cagcgatacc tgcatatcgg cagcgaagcg tcctgtttgc gtgttcatct
     1381 cttgctggcg gcagcgttcg acgaaatagt catataacgc ctctgcgcga tccggtgatg
     1441 cgcctttgga gaaacttggg cgcatccccc gttcggtatc tgcggcgagg gtaaactggg
     1501 aaaccaccag tacactgccg cccgcctgtt gcacgttgag attcatcttg ccttcggcat
     1561 cgctaaagat gcggtagccg agcacacgct cgcacagacg gtttgctttc tgttcgtcgt
     1621 catccttttc gacacccaat aacaccaaaa gtcccgcgcc aatttcgccc gtcacttctc
     1681 cctccacggt gacgctggca cgggttacgc gttgaattaa tgcaatcatg gttcgtcgtc
     1741 ttcttcttgt tcagctgctt gttttagttt gcggtattcc ccgagagtga cagtaatttc
     1801 cgcgccaagc aagacgatac accatgtcca gtagacccaa acaaagagaa tggggatcac
     1861 cgccagcaca ccgtaaatga gctgatatga cgggaacatg gtgatataaa gcgcgaaacc
     1921 tttctttcct gcttcgaaca ggagtgcggc gacaaacgcg ccgacaatcg cgtcgcggtt
     1981 aggtacgcgg atggtaggaa caatgctgta cagcaaccag aaggagatcc acgacaacag
     2041 caacggaaaa atacgcagca cgttgtcgat gacagtattg agatcgctcg cccagcgcag
     2101 ggagagcaaa taggaactga tcgccagact ggcccctgcc agcagcggcc ccagcgttaa
     2161 aatcatccag tacacggcga acgagtaaat ttttggtcgc gctcgtttac tgcgccagat
     2221 ggtattcaac gcgctatcga tggagtacat caacaataac gcggtgacga tcagcccgca
     2281 cgccccaacg gcggtcatct tgttggaatt agcaacaaat tgttcgatat accgctgaat
     2341 aacatcgcca gtagcaggca gaaagttggc aaaaataaag tgacgtaact gaatgctgac
     2401 gtcggaaaac atgggaaaag cggcgaaaag cgcaaaaaca acggcaacca gcggcactaa
     2461 tgagagcaac gacacatagg caaggttacc tgccagggtt gtcatgttgt cctcatcaat
     2521 acgttgccag agtagtttta gccaggccca tagtggacgg gtacggtgcc tggctttgtc
     2581 ctgaatggtt tttagcataa caccttcgcg aaatagtccg ggatggtggt tttatctttc
     2641 accagaatac tggtaatgcc tagctgatta gctccttcta tattatcggc gttatcgtcg
     2701 aaaaagaccg tatcgctggg tgaaaaacct tccgcctgca aaacatgctg gtaaattcgt
     2761 gcttcaggtt tgcgcatccc cagatcttgc gacagataga tatggtcagc agcatcacga
     2821 atttccgggt attcttccgg ccagaaggtg gtatgcaggc ggttggtatt ggaaagcacc
     2881 accacgcgat gcccctgctc acgcagttta tgcatgatgg cgatcacttc cgggcgcagc
     2941 gcaacaaaca ccgcctgcca gccgtgagag aactgctcgt agcttagcgg tagagccatc
     3001 tcatgacaca gcgcctctgc gaacgcttcg tcgctaattt ccccacgctc atgctgatga
     3061 aacgcctccc ccatatgaaa actcttctta agcgatgcca gcggaatacg cgttaaatcg
     3121 ctccaggctc ccagcacacg gttaaagtcg atatcgacaa tcacattacc taaatcaaag
     3181 atatagagca tttttgcctc ctttcgcgcc acaagaaatt aactgtagcg ggaaaggatg
     3241 ggtttggcta tgtggcaagg tatgaagcca ggttaacggt cacaaaaatc tacgcgtctt
     3301 cctggggaag aatgatttca atttggctgt cttgtaagcg tgattttagc gcagcgggcg
     3361 gcagctcgtc ggtaaagagc gcagtgacct gtgccacgtt accaatttca accgccgccg
     3421 aagcatgata cttagtgtga tcggcgacca gcagaatatt tctcgcgtgc gccatcatcg
     3481 ttttcaccac gttagcttcg tttacatcaa actccatcaa cgcgccatcg ctctcaatcg
     3541 ccccaacgct tgttaccaga taatcagcac gaaaatcagc cacaaaggac gccgctgaag
     3601 ggccaatgat cccgctatta tgagagcgca acgtaccgcc gggcaccatc acttcaaagc
     3661 gcgggttgtg gtaaagaata tgcgccacac gcaggctgtt ggtgattatc cgcaaatgat
     3721 tatggttaag taacgcccgg gcaacatgct caacagtcgt accaatggtg ataaatattg
     3781 ttgaaccatc aggaatatag tctgccaccg cttcggcaat cgcttttttt ttctcggttt
     3841 gcgaaacctc acgctgctcg aacgccgtat taacgacgct ggaagcccga cccgcgccac
     3901 cgtgatggcg cgtaatcagg ccttgctcgc ttaatttacg aatatcccgg cggaccgtct
     3961 gcgtggagac atccagcaga tttgccagct catcaatatt catataccca cgctcggcga
     4021 tcagcatgag gagttggtcg tgccgcgggt taccggttag ttcggtaagg ctcatgaaaa
     4081 ttcctcgaaa aaccatcacc ctggcatttt atacaaaaag tgacaaaaaa gatcgggttt
     4141 gatcacagtc agggatcccg gctcgtccac cgggacgtgt gcattttaac gccgctacac
     4201 cgctggcgaa gcggactgac tccgctaaat ccccacttgt tgccagcgcc accgccaaag
     4261 cgccgtgaaa aacatctccc gcacctgtgg tatctaccac atcaactttg aaggccggtt
     4321 gatgctgacg cccaccattt tccagccagt cgcagcctgc gctaccctgg gtcacataga
     4381 catgaccatt tgtgagcgtt tgtgcctgtt ttagcgcact ggccatctct ttcacgcccg
     4441 ttaagcgcgc cagacccggt tctgaaaagg ccgcgtgatc gcttaatgcc accagctcac
     4501 tgatatcctg cggcgtaatg tccccgtcca gaacggtcat cacacccgcc tgacgggcca
     4561 gggtgaaggc ttttttagcg ccgtcgtgcc agcgtacatc tgccagcaca acatcccact
     4621 gagagaaatc aatttcctcc aaccactctg cgtcaggcag cagatccggg ctggggtagt
     4681 taatgattat ccgctcgcct ttggtatcca ccatgatggc ggattgcgaa gatttcgcct
     4741 ggttataccg tttggtgtaa cgggtgttaa cgccccagga ttccagctct gccagcaggc
     4801 tgttgcccgc gtcgtcatca cctacgcgac caataaaatc gacctgcgcc cccagccttg
     4861 ccgccgcaac cgctgccgtc gccgctggcc cgccaccaac ttccgtataa tttctcgcca
     4921 cgtatttacc gctctccgtc ggtaaccctt ccacgtaata gatgcgatcc atcacggtta
     4981 tacctacaca agcaacacga atcatggtca ttccttaaac attttgggat gcgttcattt
     5041 taatttcacc aattgtttaa aaagtgacgg ctgtcaattt tttgacgata aattacaaat
     5101 tcgatcaaaa acagacaagc aaagcatgtc ataaatgaca aaaagtgaca tcactgtatt
     5161 caggagaggg ttatggcagc aatcgcgttt atcggtttag gacaaatggg ttcgccaatg
     5221 gcgagcaatt tattgcagca agggcatcaa cttcgcgtct ttgatgtgaa cgccgaagct
     5281 gtgcggcatc tggtagacaa aggtgcgact cccgccgcca acccggcgca ggccgctaaa
     5341 gatgccgaat ttatcattac catgttgccg aatggcgatc tggtgcgcaa cgtgttgttc
     5401 ggtgaaaacg gcgtttgcga aggcttatct accgatgcgc tggtcattga tatgtccacc
     5461 atccatccgc tgcaaaccga taaattgatt gccgatatgc aagccaaagg cttcagcatg
     5521 atggatgttc cggtaggccg tacttctgca aatgccatta ccggtactct gttactgctg
     5581 gctggcggca ccgctgaaca agttgaacgt gccacaccga tcctgatggc gatgggcagt
     5641 gagttgatca acgctggcgg tccgggcatg gggatccgcg ttaagctcat caacaactac
     5701 atgagcatcg cgctcaatgc gctttcggca gaagccgccg ttttgtgcga agccctgaat
     5761 cttcccttcg atgttgccgt caaagtgatg agcggtaccg ccgccggtaa aggtcacttc
     5821 accacttcct ggccaaacaa agtcctcagc ggagatcttt ctcccgcctt catgatcgat
     5881 cttgcccata aggatcttgg catcgccctt gatgtcgcca accagctgca tgtgccaatg
     5941 ccgctggggg ccgcctcacg ggaggtttat agccaggcgc gcgcagcggg tcgcggtcgc
     6001 caggactggt ccgccattct ggaacaggtc cgtgtcagtg ccgggatgac tgccaaagta
     6061 aaaatgtaac gactggataa aggaataaat gaatgaataa gtacaccatc aacgacatta
     6121 cgcgcgcatc gggcggtttt gccatgctgg cggtcgatca gcgcgaagcc atgcgcatga
     6181 tgtttgccgc ggctggggca cccgctccgg tagccgatag cgttttaact gatttcaaag
     6241 ttaacgctgc aaaggccctc tcgccttatg cctcggcgat tctggtagat caacaattct
     6301 gctaccgcca ggtggttgag caaaacgcga ttgccaaaag ttgcgccatg attgtcgccg
     6361 ccgatgagtt cattcctggc aacggtattc cggtcgatag cgtggttatt gaccgcaaaa
     6421 tcaatccgct acagatcaaa caagacggtg gcaaagcctt aaaactgctg gtgctgtggc
     6481 gtagtgatga agatgcgcag caacgtctgg atatggtgaa agagttcaac gaactgtgcc
     6541 actcacacgg tctggtaagc atcattgagc cagtcgtccg cccaccgcgt cgtggcgata
     6601 aattcgatcg cgaacaagcg atcatcgatg ccgccaaaga gctgggcgac agtggcgctg
     6661 acctctacaa agttgaaatg ccgctttatg gcaaaggtcc gcaacaagag cttctctgtg
     6721 cttcacaacg tctgaatgac catatcaata tgccatgggt gatcctctct tccagtgtcg
     6781 acgaaaaact gttcccgcgt gccgtacgcg tggcaatgac ggcgggcgca tcgggattcc
     6841 tggcaggccg tgcagtctgg gcatcggtcg tcggtttacc agacaacgag ctgatgctcc
     6901 gtgacgtttg cgcaccgaaa ttacaacaac ttggcgatat cgtcgacgaa atgatggcta
     6961 aacgccgcta agatgaggat aaaaggaatg aaatggttta acaccctaag ccacaaccgt
     7021 tggctggaac aggaaaccga ccgcatcttt gattttggta aaaattccgt agtgccgact
     7081 ggttttggct ggttaggcaa taaagggcaa atcaaagaag agatgggcac ccatctgtgg
     7141 atcacagctc gtatgttgca cgtttattcc gttgctgcgg cgatgggtcg acctggcgct
     7201 tacgcgttgg ttgatcacgg tattaaagcc atgaacggcg cactgcgtga taaaaaatat
     7261 ggcggctggt atgcctgcgt gaatgacgag ggcgtggtgg atgcctccaa acagggctat
     7321 caacacttct ttgctctgct gggtgccgcc agcgccgtca caacgggtca cccggaagcg
     7381 cgcaagctgc tcgattacac cattgagatt atcgagaaat atttctggag cgaagaagag
     7441 cagatgtgcc tggagtcctg ggacgaagcc ttcagcaaaa ccgaagagta ccgcggcggc
     7501 aatgccaata tgcacgcggt ggaagctttc ttgattgttt atgacgtcac tcacgacaaa
     7561 aaatggctgg atcgcgcgat tcgcgtggct tccgtgatta tccacgacgt cgccagaaat
     7621 aatcattatc gcgttaacga acacttcgat acccagtgga atccgctgcc ggattacaac
     7681 aaagataacc cggcgcatcg cttccgcgcg ttcggaggta caccaggcca ctggatcgaa
     7741 tggggccgtt taatgctgca catccatgct gccctggaag cccgttgcga acaaccacca
     7801 gcatggctgc tagaagatgc caaaggtctg tttaacgcca ccgtgcgcga tgcctgggca
     7861 cccgatggag cggacgggat tgtttatacc gttgactggg aaggaaaacc ggtggtccgc
     7921 gaacgtgtac gttggcctat cgtcgaagca atgggtacgg tctacgcgct ctacaccgtc
     7981 actggcgatc gccagtatga aacctggtat caaacatggt gggagtactg cattaagtac
     8041 ctgatggact atgaaaatgg ttcctggtgg caggagctgg atgcggacaa taaggtcatc
     8101 accaaagtct gggacggcaa acaggatatt tatcacctgc tgcattgcct ggtgatcccg
     8161 cgtatcccgt tagcgcccgg catggctcca gcggttgcag cgggtctgct ggatattaat
     8221 gcgaaataac tgatctgaaa atagggtgcg ggttttcccc tcaccctaac cctctcccca
     8281 aaggggcgag gggaccgtcc ggtgcagttt ttgggtttga catcagcctc acgggaggct
     8341 gtatgtcgtt aattaaggtg ccttgtatgc aaatcaccaa tatgcattgc agtggacaga
     8401 ccgtttcact tgccgcgggg gattatcacg cgaccattgt gaccgtcggt gctggtctgg
     8461 ctgaattaac ctttcagggt tgtcatttag tcatcccgca caaacccgaa gagatgccgc
     8521 tggcgcatct gggtaaagtg cttattccgt ggccgaaccg gatcgccaac ggctgttacc
     8581 gttatcaggg gcaggaatat cagttaccca ttaatgaaca tagttcaaaa gccgctattc
     8641 acgggctgct tgcctggcga gactggcaaa ttagtgaatt aaccaccacc agcgttaccc
     8701 tgacagcttt tctgccgccc agttacggct atccgtttat gctggcttca caagttgttt
     8761 attcgctcaa cgcacacacg ggtctttccg ttgagatcgc aagccagaat atcggcactg
     8821 tcgcagcgcc ttatggcgtg ggtattcatc cttatttaac ctgcaactta acatctgttg
     8881 atgaatatct gttccagctt ccggcaaacc aggtttatgc cgtcgatgaa cacgtaaatc
     8941 cgacaacgct tcaccacgtt gatgagctgg atttaaattt cacgcaggcg aaaaaaatag
     9001 ccgcgacaaa aatcgaccac acctttaaaa ccgctaatga tttgtgggaa atgaccatta
     9061 cccatccaca gcaggcgctt tcggtaagcc tttgttccga tcaaccctgg gtgcaggttt
     9121 atagcggtga aaaattacag cgtcagggac tggcagttga acctatgagt tgcccgccta
     9181 acgcctttaa ttcaggtatc gacttattat tactggaacc aggaaaaccg caccgcctgt
     9241 tttttaatat ttacggacag agaaaataac ttgtattgat tagcagtcaa aatcattgaa
     9301 gaacatttca ctcgctaaag gcaacattat gtatcttcaa tttattaagt taaaaacagc
     9361 tcagcttctg taaagttttt atatatcagt tttatgagaa tcattttaca taagcctgta
     9421 ataacaggaa ggcaacaatc tcgttctcaa ctctatggag attagttatg gatacgccac
     9481 gtccacagtt attagatttt caatttcatc agaataacga cagttttacc ctacattttc
     9541 aacaacgtct tattttaacc cataacaaag ataatccttg tttatggatt ggctcaggta
     9601 tagcggatat cgatatgttc cgcggtaatt tcagcattaa agataaacta caggagaaaa
     9661 ttgcgcttac cgacgccatc gtcagccagt caccggatgg ttggttaatt catttcagcc
     9721 gtggttctga cattagcgcc acgctgaata tctctgccga cgatcagggc cgtttattgc
     9781 tggaactaca aaacgacaac cttaaccaca accgtatctg gctgcgcctt gccgctcaac
     9841 cagaggacca tatctacggc tgcggcgaac agttttccta cttcgatctg cgcggcaaac
     9901 cgttcccgct atggaccagt gaacaaggcg ttggtcgcaa caaacaaacc tatgtcacct
     9961 ggcaggccga ctgcaaagaa aatgcgggcg gcgactatta ctggactttc ttcccacagc
    10021 ctacgtttgt cagcacgcag aagtattact gccatgttga taacagttgc tacatgaact
    10081 tcgactttag cgccccggaa taccatgaac tggcgctgtg ggaagacaaa gctacgctac
    10141 gttttgaatg tgctgacaca tacatctccc tgctggaaaa attaactgcc ctgctgggac
    10201 gccagccaga actgcccgac tggatttatg acggggtaac gctcggcatt cagggcggga
    10261 cggaagtgtg ccagaagaaa ctggacacca tgcgtaacgc gggcgtgaag gttaacggca
    10321 tctgggcgca ggactggtct gggatccgta taacctcttt tggcaagcgc gtgatgtgga
    10381 actggaagtg gaacagcgaa aactacccgc aactggattc acgcattaag cagtggtatc
    10441 aggagggcgt gcagttcctg gcctatatca acccgtatgt tgccagcgat aaagatctct
    10501 gcgaagaagc ggcacaacac ggctatctag caaaagatgc ctctggcggt gactatctgg
    10561 tggagtttgg cgagttttac ggcggcgttg tcgatctcac taatccagaa gcctacgcct
    10621 ggttcaagga agtgatcaaa aagaacatga ttgaactcgg ctgcggcggc tggatggctg
    10681 acttcggcga gtatctgccc accgacacgt acctgcataa cggcgtcagt gccgaaatta
    10741 tgcataacgc ctggcctgcg ctgtgggcga agtgtaacta cgaagccctt gaagaaacgg
    10801 gcaagctcgg cgagatcctc ttctttatgc gcgccggttc taccggtagc cagaaatact
    10861 ccaccatgat gtgggcgggc gaccagaacg tggactggag tctcgacgat ggcctggcgt
    10921 ccgtagtccc ggcggcgctg tcgctggcaa tgaccggaca tggtctgcat cacagcgaca
    10981 ttggcggtta caccaccctg tttgagatga agcgcagcaa agagctgctg ctgcgctggt
    11041 gcgatttcag cgccttcacg ccgatgatgc gcacccacga aggtaaccgt cctggcgaca
    11101 actggcagtt tgacggcgat gcggaaacta tcgcccattt tgcccgtatg actaccgttt
    11161 tcaccaccct gaaaccttat ctgaaagagg ccgtcgcgct gaatgcgaag tccggtctgc
    11221 cggtcatgcg cccgctgttc ctgcattacg aagacgatgc acatacctat tccctgaaat
    11281 atcagtacct gttaggccgc gacattctgg tcgctccggt acacgaagaa ggtcgcagcg
    11341 actggacgct ctatctgccg gaggataact gggtacatgc ctggacgggt gaagcgttcc
    11401 ggggcgggga agttaccgtt aatgcgccca tcggcaagcc gccggtcttt tatcgcgccg
    11461 atagcgaatg ggcggcactg ttcgcgtcgt taaacagcat ctaatcacat ccgcccgtgg
    11521 ggaaactcat gggcaaaggg agaacatcga tgagtcacat cacaacggaa gatccagcaa
    11581 ctttacgcct gccctttaaa gagaaactct cttacggtat tggcgacctg gcctctaaca
    11641 tcctgctgga tatcggtacg ctttatcttt tgaagtttta taccgacgtt ctggggctgc
    11701 caggcaccta tggcggcatt atctttttga tttcaaaatt ctttactgcg tttaccgata
    11761 tgggtaccgg cattatgttg gattcccgac gcaagatcgg tccaaaaggt aagttccgtc
    11821 cttttattct gtatgcgtca ttcccggtca ccttactggc gatcgccaac tttgtcggca
    11881 caccgtttga tgtcaccggt aaaacggtga tggccactat tctgtttatg ctttacggac
    11941 tgtttttcag catgatgaac tgctcctacg gcgcaatggt cccggcaatc accaaaaacc
    12001 ccaacgaacg cgcctcactg gcggcatggc gtcagggcgg cgctacgctg ggcctgctgc
    12061 tgtgcacggt gggattcgtg ccagttatga atcttatcga aggtaatcag aaacttggct
    12121 atatcttcgc cgccacgctg ttttcactgt tcggcctgct gtttatgtgg atctgctact
    12181 caggcgtgaa agagcgttat gtcgaaaccc aaccaaccaa tccggcgcaa aagcctggcc
    12241 tgttgcaatc tttccgcgca attgccggta accgcccact gttcattctg tgtattgcca
    12301 acctctgcac cttaggggcg tttaacgtca agctcgccat tcaggtctat tacacccagt
    12361 acgtacttaa cgatcccatc ctgttgtcgt atatgggatt tttcagcatg ggctgtattt
    12421 tcatcggcgt gttcctgatg cctggcgcag tcagacgttt tggtaagaag aaggtctata
    12481 tcggcggcct gctgatttgg gtgctgggcg atctgctcaa ctatttcttc ggcggcggct
    12541 cggtcagctt tgtggcgttc tcctgcctgg cgttcttcgg ctcagcgcct gaactgggcg
    12601 ctggtttccg acaccgtcga gtacggcgag tggcgcaccg gcgtgcgttc ggaaggcacg
    12661 gtctacaccg gcttcacctt ctttcgcaaa gtgtctcagg cgctggctgg tttcttcccc
    12721 ggctggatgc tgacgcaaat tggctatgtg ccgaacgtcg cacaggctga ccacaccatt
    12781 gaagggttac gccagttgat cttcatctac ccaagcgcac tggcggtagt caccattgtg
    12841 gcgatgggtt gcttctacag cctgaacgag aagatgtatg tccgcattgt ggaagagata
    12901 gaagcccgta aacgcacggc gtaataataa taaacgacgc cctgcggggc gttataagga
    12961 gtgattatgt ctgaccataa tccactgaca ttaaaactga atctgcggga aaaaatcgcc
    13021 tatggtatgg gcgacgtcgg ttcgaattta atgctctgca tcggtactct gtatctcctc
    13081 aaactttata ccgatgaatt agggatgcct gcttactatg gcgggattat ctttctggtc
    13141 gcgaagtttt ttaccgcgtt taccgatatg ctcaccggat ttttactcga ctcgcggaaa
    13201 aatattgggc ctaaaggcaa atttcgtccc tttattttat atgcagcagt tccggcggcg
    13261 ttaattgcaa cgctccagtt tatcgccacc accttttgtt tgccggttaa aacgacgatt
    13321 gccaccacgc tgtttatgat gtttggactt tcatacagtc tgatgaactg ctcgtatggt
    13381 gcgatgatcc cggcaattac caaaaacccg aatgagcgcg cgcagctcgc ggcttaccgc
    13441 cagggtggtg caaccatagg gctattgatt tgtaccgtgg cgtttattcc gttgcagtcg
    13501 cttttttctg actcaaccgt cggttatgcc tgtgcggcac ttatgttctc cattggcggc
    13561 tttattttta tgatgctgtg ctacagaggc gtcaaagagc attatgtgga cacagcgcca
    13621 accggacata aagccagtat tctcaaatct ttttgcgcga tatttcgtaa tccaccattg
    13681 ctggttttat gcattgctaa cctgtgtact ctggcggcat ttaatatcaa actggcgatt
    13741 caggtctatt acacccagta tgtgctgaat gatattaatt tattgtcgtg gatgggattc
    13801 ttcagtatgg gatgcatcct cgtcggcgta ttactggtgc cagtaactgt aaaatgtttt
    13861 ggtaaaaaac aggtttattt agctggcatg gtgctatggg cggtgggtga tatactgaat
    13921 tatttctggg gaagtaactc tttcactttc gtcatgttct cttgtgtcgc cttttttggc
    13981 acggcctttg taaacagcct gaactgggca ctggtaccag ataccgtaga ttacggtgaa
    14041 tggaaaaccg gtattcgtgc cgaaggttct gtttataccg gttatacctt ctttcgtaaa
    14101 atttctgccg cacttgctgg cttcttgcca ggcattatgc tgacgcaaat tggttatgtt
    14161 cccaacatcg cgcaaagcga tgcgacatta cagggtttgc gtcagctcat ttttatctgg
    14221 ccttgtgcac tggcaattat tgctgcattg acgatgggat tcttttacac actcaatgaa
    14281 aaacggtttg cattgattat tgaggaaatc aaccaacgca aaaataaaga gatggcgaca
    14341 gaaaaaaaaa cggcttccgt aacgttataa atgtcaatag ccgctatttc catcctggtg
    14401 ggtggcggcc tccctacgtt taaaaaatgg acttattatg aaaaagatta atgcaataat
    14461 tttattatct tctctgacct ctgcctcggt atttgctggc gcatacgtcg aaaacaggga
    14521 ggcatataat cttgcctccg atcagggcga ggttatgcta cgtgtgggtt ataacttcga
    14581 tatgggcgcg ggtattatgt taaccaatac ctacaccttt cagcgagaag atgaactaaa
    14641 acatggctat aatgaaattg aaggttggta tcctttattc aaaccaaccg ataaattaac
    14701 catccagccc ggtggcttaa ttaatgataa gagtatcagt tcgggtggtg cagtgtatct
    14761 ggac
//
LOCUS       DAZSNM010000094        14624 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZSNM010000094 DAZSNM010000000
VERSION     DAZSNM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14624
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.094"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="ACHW42_002370"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="ACHW42_002370"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR8944168.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKPPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="ACHW42_002371"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="ACHW42_002371"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR8944169.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="ACHW42_002372"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="ACHW42_002372"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR8944170.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="ACHW42_002373"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="ACHW42_002373"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR8944171.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4817
                     /gene="citX"
                     /locus_tag="ACHW42_002374"
                     /pseudo
     CDS             4267..4817
                     /gene="citX"
                     /locus_tag="ACHW42_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4792..5670
                     /gene="citG"
                     /locus_tag="ACHW42_002375"
     CDS             4792..5670
                     /gene="citG"
                     /locus_tag="ACHW42_002375"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR8944172.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5721..7183
                     /gene="citT"
                     /locus_tag="ACHW42_002376"
                     /pseudo
     CDS             5721..7183
                     /gene="citT"
                     /locus_tag="ACHW42_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7297..8103
                     /gene="rna"
                     /locus_tag="ACHW42_002377"
     CDS             7297..8103
                     /gene="rna"
                     /locus_tag="ACHW42_002377"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR8944173.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8332..8742
                     /gene="rnk"
                     /locus_tag="ACHW42_002378"
     CDS             8332..8742
                     /gene="rnk"
                     /locus_tag="ACHW42_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR8944174.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8880..8969
                     /gene="yldA"
                     /locus_tag="ACHW42_002379"
     CDS             8880..8969
                     /gene="yldA"
                     /locus_tag="ACHW42_002379"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR8944175.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8973..10211)
                     /locus_tag="ACHW42_002380"
     CDS             complement(8973..10211)
                     /locus_tag="ACHW42_002380"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFR8944176.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10432..10860
                     /gene="uspG"
                     /locus_tag="ACHW42_002381"
     CDS             10432..10860
                     /gene="uspG"
                     /locus_tag="ACHW42_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR8944177.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="ACHW42_002382"
     CDS             complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="ACHW42_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR8944178.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="ACHW42_002383"
     CDS             complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="ACHW42_002383"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR8944179.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13726..14472
                     /gene="dsbG"
                     /locus_tag="ACHW42_002384"
     CDS             13726..14472
                     /gene="dsbG"
                     /locus_tag="ACHW42_002384"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFR8944180.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3541 a         3838 c         3630 g         3615 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcaaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agccccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ccacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgcgcc tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc gcgcgtggaa
     4741 aaacccatca actgaccgat ttactcaacc gcatggaggc actgctgaac gatgtcgatg
     4801 cctgcaacgt caactaaaac cacaaagctt gcgacgtcat taatcgatga gtacgccctg
     4861 ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg cctcgtggat
     4921 cgcattaact gcggtgcgca caaagatatg gcgctggaag atttccaccg cagcgcgctg
     4981 gcgattcagg gctggttacc ccgtttcatt gaatttggtg cctgtagtgc agaaatggca
     5041 ccggaagcgg tactccacgg attacgccca attggtatgg cttgcgaagg tgatatgttc
     5101 cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg gctgctatgt
     5161 gcggcaattg gccgtttgct tcagctcaac cagccggtta cgccaacaac cgtttgttct
     5221 acggcggcaa gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa taattcacga
     5281 ctgacggcag gtcaacggtt gtaccagcag cttggcctta ccggcgcacg cggtgaagcc
     5341 gaagcaggtt atccactggt gatcaaccac gccttgccgc attacctcac tctgctggat
     5401 caggggttag atcctgaact ggcattgctc gataccttgc tcctactgat ggctaccaac
     5461 ggcgatacca acgttgcatc gcgcggtggc gaggggggcc tgcgctggct acagcgcgag
     5521 gcgcaaacat tattgcaaaa agggggcatt cgaaccccca ccgatctcga ttatctccgg
     5581 cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc cgacctgctg
     5641 atccttacct gttttttagc acagatttaa ttatttaagc acttgataaa tttggaaata
     5701 ttaattttcg gagaacccgt atgtctttag caaaagataa tatatggaaa ctattggccc
     5761 cactggtggt gatgggtgtc atgtttctta tccctgtccc cgacggtatg ccgccgcagg
     5821 catggcatta cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc gagccaattc
     5881 cggcaacagc gatcagtttt attgcggtta ctatttgcgt tattggcagt aattacctgc
     5941 tctttgatgc caaagaatta gctgacccag cgtttaatgc gcaaaaacag gcgctgaaat
     6001 ggggcctggc tggtttttcc agcaccacgg tatggctggt atttggcgca tttatttttg
     6061 cattagggta tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg gtgaaattca
     6121 tggacaaacg cacgctgacg ttgggttatg cgattgtcat tatcgacatt ctgctggcac
     6181 cgtttacacc gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc attaaaaacc
     6241 tgccgccgtt gtttaaatca ttcccgaacg atccgtccgc gcgtcgtatt ggcggctatt
     6301 tgatgtggat gatggtcatt agtaccagtc tgagttcgtc catgtttgtc accggtgcgg
     6361 accaaacgtg ctgggtctgg agttcgtcag caaaattgcc ggtatccaga ttagctggtt
     6421 gcagtggttc ctctgcttcc tgccggttgg ggttatcttg cttatcattg cgccgtggct
     6481 ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa cctgggcggg
     6541 tgatgaacta aaaaccatgg gtgcgctgac acgcagagag tggacgctga ttggccttgt
     6601 attgctcagc ttaggtttgt gggtatttgg cagtgaagtc attaatgcta ctgcggttgg
     6661 tctgctggca gtttcgctaa tgctggctct gcacgttgtg ccgtggaaag acattacccg
     6721 ctataacagc gcatggaaca cgctggtcaa cctggcaact ctggttgtga tggctaacgg
     6781 cctgactcgt tctggtttta ttgactggtt cgccggtacc atgagtacgc acctggaagg
     6841 attctcacca aacgcaacgg tgattgtact ggttctggtg ttctactttg cacactacct
     6901 gtttgccagc ctgtctgcgc acaccgcaac catgctgccg gttattctgg ccgtcggtaa
     6961 aggtattccg ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt ctatcggtat
     7021 catgggctgt ctgacgccgt atgcaaccgg tcctggggtg attatttacg gctgtggcta
     7081 tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc ggggtgattt acatctctat
     7141 gttgctgttg gttggctggc cgattctcgc catgtggaac taatacgttc gccaaacgct
     7201 gaataaatca tcacgcccgc caggtaagcc cacctggcgg gcttttttat gatttaatag
     7261 atagtattca gtgtatgtat gagttccaca cccattatga aagcattctg ccgtaacgcc
     7321 gcgttgctcg cggtttctct gcttccctta tcttctgcca acgccgtagc gttgcaggca
     7381 aaacagtatg gcgattttga tcgctatgtc ctggccctct cctggcaaac cggattttgc
     7441 cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg
     7501 accaacaaaa ctgattttct gaccgtacat ggtctgtggc caggattgcc taaatcggtt
     7561 gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc
     7621 ccgaatctac cagaagcacg cgccagccga atgtgttcat cgccggaaac cgggttatca
     7681 ctggaaacgg ccgctaaact aagtgaagtc atgccaggag ctggcggacg ttcctgcctg
     7741 gaacgctacg aatatgccaa acacggtgcc tgctttggtt ttgatccgga cgcatacttc
     7801 ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa attccttgcg
     7861 gataattacg gtaaaacagt gagccgccgt gactttgacg ccgcctttgc caaaagctgg
     7921 ggaaaagaga acgtgaaagc agttaagcta acgtgccagg gtaaccctgc gtatttgact
     7981 gaaattcaga tctcgatcaa agctgacgcc atcaacgctc cgctttctgc aaactcattc
     8041 ttgccacaac ctcacccagg taactgtggc aaaacctttg tgattgataa agcgggttat
     8101 taatcctctg atttacaaaa cacgctaatt cgtcttccat aaccatgccg attaagaatt
     8161 atacataatg cgctgcattg aggaattccc tgaatgtgac gcaaatcact tcaaagagtg
     8221 agcaacagtc agtatcatca tgtgagttaa accctcgccg cctgacggtg agggttttct
     8281 tttgggtatg tttatctgcg acactcgcag taccgacgac atggagtaaa aatgtccaga
     8341 ccaactatca tcattaacga cctggatgcc gaacgcatcg atattctgct ggagcaaccc
     8401 gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag agttggatcg cgcccaaatg
     8461 tgttcgccag aagagatgcc acacgacgtg gtgacaatga acagccgggt taaattccgc
     8521 aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa aatgaccgat
     8581 agcaatactc agctttccgt tatggctccg gtaggtgccg cactgctggg gctgcgcgtt
     8641 ggcgattcca ttcactggga acttccgggc ggcgttgcaa cccaccttga agtgctggaa
     8701 ctcgaatacc agccagaagc tgctggcgac tacctgcttt aatcccatct gcacaagcga
     8761 taactcgctt tgcccagagg atgcactcgc atcctcttta cgcaaaatca gcaatttttc
     8821 ctggttctca ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg ggaataacca
     8881 tggcagaagc attttacatc ctgataggat ttctgattat ggcggcgatt atcgtcatgg
     8941 ctgtccttta cctcgaaaac cactcctgac gatcatattg ttccccccgg catcgcattc
     9001 accagacctg aaaccgcctt ctgcgccgcc tctgcgcttt gtgcaccggg taccagaatc
     9061 accttacggc actcctcttc acgtttttcg aaaatctcat atccccgggc ggcctcttca
     9121 aacggcatat agtgggtaac aatttcttct ggtttcagta atcctttctc aattaacggt
     9181 aataattctc ccagccatgc gtgaacgtgg gtctgtccca ttttaaacgt caacccttta
     9241 tcaaaggcgt cgccaaacag gaaaccgtga ataaatccag cgtagacgcc cggtacgcta
     9301 acaatgccgc cacgcctgac cgccgcaata cactggcgca acgctttacc gctgctgccc
     9361 tccagtttca ggttagtcag caccgtttcc gtggtgctgc ctttcgcttc aaaaccgacg
     9421 gcgtctatta ctgcatccac gccccggtga cctgccgttt gttcaataat tgactgtgcc
     9481 ggatcgctat cttcatcgaa attaatcggg atcgcgccgt agcggtcagc ggcgaaacgc
     9541 aagcggtagg gatgatgatc aacaacaaaa atctgttccg caccgagcaa ccgtgcacag
     9601 gcgattgtca acaatcccac aggaccagca ccatagactg caacgcttga accttgttgg
     9661 atctgcacat tttttgctgc ctgccatgcc gttggcagaa tatcagaaag gaaaagggct
     9721 ttatcatctg aaagcaaagg cggtacttta aacggcccca cattcccttt cgggacgcgg
     9781 acatattccg cctgcccgcc aggaacgcca ccatacaggt gactataacc aaacaatgcc
     9841 gcgggagcag gtatctgttt tttattgagc gcagcgcctt tacccgcatt ggtattttcg
     9901 caggcggcat attgctgtaa tcgacagaaa aaacagtcgc cacaagcaat gacgaacgga
     9961 attaccaccc ggtcgccttt ttgcaaattt tttacgtcct tcccggtttc aacgacttcc
    10021 cccataaatt catgaccaaa aatatcgcca tgtttaacct ggggtatttt gcctcgataa
    10081 agatggaggt cagagccaca gatcgccgtt accgtaatac gcagaataat atcatctgcc
    10141 tgttcaatgc ccggatcggg aacattttct acctgaacgt gatgtgggcc gtgataagtc
    10201 aatgctttca tgcaacctcc agagacagtt caaaaaagag tcagtcttta agggtagcta
    10261 tcacagcggg aaaacgggcg gtaccgcagg ttttgggaga attcctagtg ttcagtgaga
    10321 atatattaat cacagaaacc gttcgtaagg aaaaagaacc agacaaatat cagggataaa
    10381 tattataatc atggagaatg atttgttcat gattaacagg gagaaagggt tatgtataag
    10441 gcaatcatta tgccagttga tgtatttgaa atggaattga gcgacaaagc tgttcgccac
    10501 gctgaattcc tcgcccagga tgacggagtt attcatctac ttcacgtact acccgggtca
    10561 gccagcctga gcctgcaccg ttttgccgct gatgtgcgtc gttttgaaga gcatctgcaa
    10621 catgaagcag aagaacgtct gcaaacgatg gtcagccact tcaccatcga tccttcccgc
    10681 attaaacaac atgtccgttt tggtagcgtg cgggatgaag tcaatgagtt ggcagaagaa
    10741 ctgggggctg atgttgtagt tatcggttct cgcaacccat cgatttcgac ccatctgtta
    10801 ggttctaacg cctcgagcgt gatccgccac gccaatctgc ctgtactggt cgttcgctaa
    10861 cagataaaag tatttatgcc gccctgcctg gaacatcccg gcagggcttt ttaatgccat
    10921 aaaaaagccg ccaggtttga ccccggcggc taagcaattg caggtgaatc ttacttcttc
    10981 ttatgcagtt ttggtgcgaa tcaggtagtc aaaagcactc agagaggctt tggcaccttc
    11041 gccagtggcg atgatgatct gcttgtacgg aaccgtcgta cagtcacccg ctgcgaacac
    11101 gcctttgacg ttggtttcac atttcgcatc aatgataatc tcgcccatgc ggttacgttc
    11161 gactgcgcct tccagccagt tggtgttcgg cagcagacca atctggacaa aaataccggc
    11221 cagttcgatg ttgtgaatat tgccgctgac acgatctcga tattccagac caacgacttt
    11281 gctgccgtcg cctttcactt ccgtggtttg cgcattcaga ataatgtcga cgtttttcag
    11341 gctgcgcagt ttgtcctgca gaacctggtc ggctttcatt tctggcgcaa attccagcag
    11401 cgttacgtgc tcaacgatac ccgccaggtc gattgccgct tccacgccgg agttaccgcc
    11461 gccgataacc gctacgcgtt tgcctttaaa cagcgggccg tcgcagtgcg ggcagtaggt
    11521 cacgccttta gtgcgatact gatcttcgcc cggaacgttc atgttgcgcc attttgcacc
    11581 cgtcgccacg ataatgctgc gtgctttcag taccgcgcca gaagctgttt caatctggtg
    11641 tagaccacct tcaaccgctg ccgggatcag tttgctggcg ctctggctgt cgatcacatc
    11701 aacgtcgtat tcatcaacgt gaactttcag tgcgcctgcc aacttctgcc cttcagtctt
    11761 cggtacagaa atgtagtttt cgatatcaac ggtatcgagg atctgaccac caaaacgttc
    11821 gcccatcaga ccggtacgga tgcctttacg tgcggagtaa attgctgccg ctgcacccgc
    11881 cgggccggaa ccaacgatta atacgtcata agcatcacgc ttgttcagct cttctgccgc
    11941 acgattttcc gcgccagtat caattttggc aacgatttca gtcaacgtca tgcggccctg
    12001 accaaactct ttcccgttta cgaacactgc cggaacgccc atcacgttgc gatcggtgat
    12061 ttcgttctgg aaggtgccgc cgtcaattgc agtgtgcttg atgcgcgggt tcaatacgct
    12121 catcaggttc agcgcctgca ccacgtccgg gcagttatgg caagagagcg agtaataggt
    12181 ttcgaattca aaatcaccgt caatatggcg aatctgctcc agcagagact gcgcttcttt
    12241 cgacggatga ccaccggtcc acagcaacgc cagtaccagc gaggtgaact cgtggcccag
    12301 cggggagcct gcaaaacgtg gtccctggtt ggaacctggg ttggtgatca ggaaagacgg
    12361 cttacgcact ggcaagctgt tatcttcttt aaaggtgact ttgtctgaca gttctgcgat
    12421 ttcagccaac agttccttga tttctgccga tttagcgctg tcatccagcg tggcaattaa
    12481 ctcaacaggc ttggtcaatt tctcaaggta agccttgagt tgagttttca tatttgtgtc
    12541 gagcatgttt atctcctggg ctttaaacat catcatgcaa gcggcctctg cggctacctg
    12601 gatgcagctt gcatcatggt gcggggaaga aaatcgggtg ccgcagcacc cgaagaatta
    12661 ggtgaatttc cgaagtattt cgcgtcagag caaggcgact agcctgtgaa tccccgggag
    12721 cttacacaag taagtgaccg gggtgagcgg gcgacgccaa cgccgctatg gcgtgaaaga
    12781 cgaaggaaat ttagatttta ccaaccaggt ccagagacgg agccagagtt gcttcacctt
    12841 ctttccattt agccgggcaa acttcacctg ggtgagaagc tacgtactga gctgctttga
    12901 ttttacgcag cagatcagac gcgtcacggc caatgccttc agcggtaact tcgattgcct
    12961 ggatgatacc ctgcgggtca acaacgaagg tcgcacggtc agccagacct tcatcttcac
    13021 gcatgttgtc gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc gcatatttga
    13081 ttttagcgat ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga gtatcggtag
    13141 atactgcgta tacgtctacg cccagtttct gcagttcttc gtagtggtca gcaacgtcac
    13201 ccagttcggt cgggcatacg aaagtaaagt cagccgggta gaagaagaag acgctccagc
    13261 ggccttcggt atctttttcg gtgatttcga tgaattcgcc gtttttgaat gcctggtttt
    13321 taaaaggttt aattttggtg ttaatcaagg acatctatac ttcctccgtg ttttcgatga
    13381 gatgtaaggt aaccaatttt tgccgattcg gctaatgcgt ttccattatc aaatcgataa
    13441 gttttacctt acaaccttcg taagacaact ttgtgaactt tgcaccgcca gcggcgataa
    13501 aaagtaaaat ggcccccttt ccaggtgacc gtgtaatctg aactaccctt tggtatcttc
    13561 agcgcctgcc tgcaggctga ggtgttgccc ttacaaatgc aacaacgaca tggattacaa
    13621 caccctcata aacaaagggc aatcacctga tctaagctct tacctatgac agtgataggt
    13681 tatgcctttt actcgacttt tgcactgact gaaaagaaca aattaatgtt aaaaaagata
    13741 cttttactgg ctctgcttcc tgcaatcgcc ttcgcagagg aacttccttc cccagtaaaa
    13801 gcgattgaaa aacagggcat tacaatcatc aaaacgttcg atgcccccgg aggaatgaaa
    13861 ggttatctcg gaaagtatca ggatatgggc gtcaccatct acctgactcc cgatggtaag
    13921 cacgctatct ctggttacat gtacaacgag aaaggtgaaa acctgagtaa cacacttatc
    13981 gaaaaagaaa tttacgcacc agccggacgc gaaatgtggc aacggatgga acaatcccac
    14041 tggctcctcg acggtaaaaa agatgcgccg gtcattgtct acgtcttctc cgatccgttc
    14101 tgcccatatt gtaaacagtt ctggcagcag gcgcgcccgt gggtagattc tggcaaagtg
    14161 caattaagaa cattgttggt tggggttatc aagccagaaa gcccggcgac agcagcggca
    14221 attcttgcct ccaaagatcc cgcaaaaacc tggcaacaat atgaagcctc tggttgcaag
    14281 cttaagctaa acgtgcctgc aaacgtaagt acagagcaaa tgaaagtgtt aagtgacaat
    14341 gagaaactga tggacgatct gggggcaaat gtcacgccgg ctatctatta catgagtaag
    14401 gaaaatacgc tgcaacaggc cgtggggttg cccgatcaga aaacgcttaa tatcattatg
    14461 ggaaataaat aaaaggtaat tcatggatgg ttgaactata tccctgcttt tcaaccatcc
    14521 taaaaaagca tgaatttctc gctctaccct aagtggctgt gctattttag aaaaacttaa
    14581 tcctgagtta agcaaaaaaa cataatcaat aaaaatacat acgc
//
LOCUS       DAZSNM010000095        14553 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZSNM010000095 DAZSNM010000000
VERSION     DAZSNM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14553
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.095"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            230..796
                     /locus_tag="ACHW42_002385"
     CDS             230..796
                     /locus_tag="ACHW42_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8944181.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            847..3306
                     /locus_tag="ACHW42_002386"
     CDS             847..3306
                     /locus_tag="ACHW42_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR8944182.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3321..4052
                     /locus_tag="ACHW42_002387"
     CDS             3321..4052
                     /locus_tag="ACHW42_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR8944183.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            4049..5125
                     /locus_tag="ACHW42_002388"
                     /pseudo
     CDS             4049..5125
                     /locus_tag="ACHW42_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            5277..6323
                     /locus_tag="ACHW42_002389"
     CDS             5277..6323
                     /locus_tag="ACHW42_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR8944184.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6320..7111)
                     /gene="nei"
                     /locus_tag="ACHW42_002390"
     CDS             complement(6320..7111)
                     /gene="nei"
                     /locus_tag="ACHW42_002390"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR8944185.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7147..7881)
                     /gene="pxpA"
                     /locus_tag="ACHW42_002391"
     CDS             complement(7147..7881)
                     /gene="pxpA"
                     /locus_tag="ACHW42_002391"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR8944186.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7871..8803)
                     /gene="pxpC"
                     /locus_tag="ACHW42_002392"
                     /pseudo
     CDS             complement(7871..8803)
                     /gene="pxpC"
                     /locus_tag="ACHW42_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(8797..9453)
                     /gene="pxpB"
                     /locus_tag="ACHW42_002393"
     CDS             complement(8797..9453)
                     /gene="pxpB"
                     /locus_tag="ACHW42_002393"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR8944187.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(9476..10219)
                     /gene="ybgI"
                     /locus_tag="ACHW42_002394"
     CDS             complement(9476..10219)
                     /gene="ybgI"
                     /locus_tag="ACHW42_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR8944188.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10490..11971
                     /gene="dtpD"
                     /locus_tag="ACHW42_002395"
     CDS             10490..11971
                     /gene="dtpD"
                     /locus_tag="ACHW42_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR8944189.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(12121..13539)
                     /gene="phrB"
                     /locus_tag="ACHW42_002396"
     CDS             complement(12121..13539)
                     /gene="phrB"
                     /locus_tag="ACHW42_002396"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR8944190.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(13536..14045)
                     /locus_tag="ACHW42_002397"
     CDS             complement(13536..14045)
                     /locus_tag="ACHW42_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR8944191.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            14174..14461
                     /locus_tag="ACHW42_002398"
     CDS             14174..14461
                     /locus_tag="ACHW42_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HFR8944192.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
BASE COUNT         3565 a         3885 c         3516 g         3587 t
ORIGIN      
        1 ttttaatcat ctatcttcct ctcagttaca tgaataaacc accaaatata gcgcacccct
       61 ataattccca atactttatt catcaaacat ttaattcata ccatcaatta attactttta
      121 catttaataa aattaataat atttttaaga aaaactaaaa acattcgaca atcaaataag
      181 atattcttgc accgatatac ttttttcttt tctgttaaag gactcagtta tgtttaaagg
      241 acaaaaaaca ttggccgcac tggccgtatc tctgctgttc acagcacctg tttatgcggc
      301 tgatgaagga tccggtgaaa ttcactttaa aggtgaagtt attgaagcac cgtgtgaaat
      361 acatcaggat gatattgata aagaggttga actcggtcag gtgaccacca gccacattaa
      421 tcagtcacat cacagcgatg ccgttgctgt cgacctgctc ttagtcaact gtgatctgga
      481 aaactccagc aacggttccg gtggcaagat ttctaaagtt gcagtcacct ttgatagctc
      541 agcgaaaacc accggcgcag atccgattct caacaacacc agcacgggtg aagccaccgg
      601 cgtcggcgta cgtttaatga ataaagatca aagcaatatc gttttaggca ccgctactcc
      661 agatatcgac ctggctccga cctccagcga acaaacgctg aatttctttg cctggatgga
      721 acaaattgat caggctacac ctgtaacgcc aggcgcagtt acggcgaatg caacctacgt
      781 gctggattat aaataagtat tactttggcg gcgctatcgc cgcctttcat aaaatattta
      841 tcaggtatgg acaccgtgaa tatttatcga ctctcttttg tatcctgcct ggtcgtggcg
      901 atgccttgcg cattggcggt cgaattcaat ctgaatgttc tcgataaatc aatgcgcgac
      961 cgcattgata tttcattatt aaaggaaaaa ggagtcattg ctcccggtga gtattttgtt
     1021 agcgttgcgg taaataataa ccaaatcagt aacgagcaaa agattaactg gcacaaaaat
     1081 gacgataaaa ccattccgtg catcaatgat ttactggtcg ataaatttgg cttaaaacct
     1141 gaagtccgtc agtcgttacc attgataaat cagtgcgtcg attttagctc ccgacctgaa
     1201 atgctcttca atttcgatca agccaatcag caactgaata ttagtattcc gcaagcctgg
     1261 ctggcgtggc actcagaaaa ctggacccca ccctccacat ggaaagaagg tgtcgccggt
     1321 gtcctgatgg attacaactt gtttgccagc agctaccgcc cacaggatgg cagcagcagc
     1381 actaatctta acgcctatgg aacagccgga atcaacacgg gggcatggcg cttacgcagt
     1441 gattatcagt tgaatcatac tgatagcgat gataaccatg aacagtcagg cgaaatatca
     1501 cgtacctatc tttttcgccc gttaccgcaa ttaggttcaa aattaaccct cggcgaaacg
     1561 gattttagtc ccaatatttt cgacggtttt tcttataccg gcgcggcact ggcaagtgac
     1621 gatcgaatgt tgccatggga actacgcggc tacgccccac aaattagcgg tattgcacag
     1681 accaatgcca cggtgacgat cagtcaatca ggccgcgtca tttaccagaa aaaagtccca
     1741 ccaggcccat ttatcattga cgaccttaat cagtctgttc agggcacact ggatgtcaaa
     1801 gtgacggaag aagatggtcg ggtgaacaat ttccaggttt cagcagcatc gacgcccttc
     1861 ctgactcgtc agggacaggt tcgctataaa ctggccgcgg gtcagccacg gccctccatg
     1921 tcacatcaaa ctgaaaatga aacctttttt agcaatgaag tttcctgggg gatgctgtca
     1981 aacacctcgc tgtacagcgg cctgctgctt tctggtgatg actaccattc tgccgcaatg
     2041 ggtattgggc aaaatatgct gtggcttggt gcgctgtcgt ttgatgtcac gtgggccagt
     2101 agccattttg atactcagca ggacgagcgg ggcttaagct accgttttaa ttacagcaaa
     2161 caagtggatg ccactaacag cacgatttcc ctcgccgctt atcgtttctc cgatcgtcat
     2221 tttcacagct acgccaacta tctggatcac aaatacaacg acagcgatgc gcaggacgaa
     2281 aaacagacga tcagcttatc tgtgggccaa ccgattaccc cactaaacct caatctttac
     2341 gccaacctgc tacatcaaac ctggtggaat gcagacgcct ccacgaccgc caacatcacg
     2401 gcaggtttta atgttgatat tggtgactgg agagatatct ctatttctac gtcattcaat
     2461 acaacccatt acgaagataa agatcgcgac aaccagattt acctgtcgat ttcgctcccc
     2521 ttcggtaacg gtggtcgggt tggctatgac atgcaaaaca gtagccacag caccacacac
     2581 cgcatgtcgt ggaacgatac gctggatgaa cgtaatagct ggggcatgtc tgccggactg
     2641 caatccgacc gtcctgacaa tggagcccag gtgagcggta actatcagca cctgagttca
     2701 gcgggtgagt gggatatttc tggtacctat gccgccaatg attacagttc cgtcagcagc
     2761 agctggagcg gttctttcac cgcaacccaa tatggtgcag cgtttcatcg ccgcagctcc
     2821 accaatgaac cacgcctgat ggtcagcacc gatggcgtgg cagatattcc ggttcagggc
     2881 aatctcgact acaccaacca ttttggcatt gcggtggtgc cgttgatttc cagttaccag
     2941 ccttccaccg tggcggtgaa catgaatgac ttacccgacg gcgtaacagt tgcagaaaac
     3001 gttatcaaag aaacgtggat tgaaggcgcg ataggttaca aatcactggc ttcccgttcc
     3061 ggtaaagacg ttaacgtcat cattcgcaac gccagcggtc agtttcctcc cctcggagcg
     3121 gatatccgcc aggatgacag cggcattagc gtggggatgg ttggcgagga aggacatgcc
     3181 tggttaagcg gagtcgctga aaatcaaaag tttaccgtgg tctggggtga tagccagcat
     3241 tgctcgctcc atcttcctga acatatggaa gacaccgcaa atcgcctgat tttaccttgt
     3301 cattaattaa ggaaatgaaa atgacattta tgaaaggact accccttttg ttgttggttg
     3361 ccagtctgtg cagccacgct gcactacaac ccgatcgcac tcgtattgtg tttaacgcta
     3421 atgataaagc taccagtctg cgggtggata atcgtagcga taaactcccc tacctcgctt
     3481 attcctggct cgaaaatgaa aaaggggaga aaagcgatga tcttctggtc gccctgccgc
     3541 cgatacaacg tctggaaccg aaagcaacaa cacaggtacg gatagtaaag caagcctcga
     3601 ccacaaaatt accgggcgat cgcgaaacgc tatttttcta caatatgcgt gaaattcctc
     3661 ccgccccgga aaaaaacagc gaccatgccg ttctgcaagt cgccattcag agtcgtatca
     3721 aggtgttctg gcgaccggct gcattacgca agaaagcagg agaaaaggtc gaactgcagt
     3781 tacaggtcag ccagcagggc aaccaactga cactgaaaaa tcctaccgcg tattatctga
     3841 ccattgccta cctgggacga aatgaaaaag gcgttctccc cggcttcaaa accgtgatgg
     3901 ttgcgccttt tagtacggtc aacacaaaca ccggaaatta tagcggcagc cagttttatc
     3961 tcggttatat ggatgattac ggcgcgttac gcatgacgac gctcaactgc agcggacaat
     4021 gccatttaca agccgtggag gcgaagaaat gagtgctggc aagggagtgt tgctcgtcat
     4081 ctgtctgtta tttctgtcgt tgaagtctgc gcttgccctg aactgctact ttggttcatc
     4141 cggtggctcg gtagaaaaat cagaagcgat tcaacctttt gctgtaccag gcaatgccaa
     4201 actcggcgat aagatctggg aatctgacga tattaaaata agatctggga atctgacgat
     4261 attaaaattc ccgtctattg tgacaacaat accaacggca actttgaaag cgaacatgtc
     4321 tacgtatggg taaaccccta cccgggggtg caggatcgtt attatcaact cggagtaact
     4381 tacaatggcg ttgattatga cgccaaccag ggaaaaagcc gcatcgacac taaccagtgt
     4441 atcgacagta agaacattga catttatacc cctgatggga tggcagaaca aaatttgctc
     4501 gggtgatccc gctaatattc acatgtcgcg aacctttctc gctcgcatgc ggttatacgt
     4561 caaaatacga gaaatgccgc cgcatgatta tcaaagtacg cttagcgact atatcgtcgt
     4621 gcaatttgac ggtgccggta gcgttaacga agaccctact gcccaaaacc tgaaatatca
     4681 tattactggt ctggaaaaca ttcgcgtgct ggattgcagc gtcaattttt ccatttcacc
     4741 ggaaacacag atgattgatt ttggtaaatt taatttgctg gatattcgtc gccacacaat
     4801 gtcgaaaaca ttcagtatta agacgacaaa atcacaaaat gaccaatgca ccgacggatt
     4861 taaagtcagt tcctcctttt ataccgaaga aacgttggtt gaagaagata aagcattgtt
     4921 gattggtaat ggtctgaagc tacgtttgct ggatgaaaac gcctcgcctt ataccttcaa
     4981 taaatatgct gaatacgccg atttcaccag cgacatgttg gtctacgaaa aaacctatac
     5041 agctgaactt tcgtccattg caggtacccc catcgaggct gggcccttcg atacagtggt
     5101 gctttttaaa attaactata actgatttgc catgatgccc tttgcagccg gaaagggcat
     5161 ctctttcaga aaattgctga ttttcccctt ttccttgatt gctgttattt tttcctagcc
     5221 cggctttctt tatgctgtat cgatacacca aagagaaaat aatgagggag cgaaggatgc
     5281 cagttttgca gtggggaatg ttatgtgtgc tatcacttct cctttctatt ggtttcctcg
     5341 cggtacatct cccggcggcg ctattacttg ggccgatgat cgccgggatc atcttcagta
     5401 tgcgcggaat tactctgcaa cttccccgct ccgcttttct cgccgcgcag gccattcttg
     5461 gctgcatgat tgcgcaaaac ctcactggtt cgattctcac cacgctggcc gccaactggc
     5521 cgatcgtgct tgctatttta ctggtgacgc tgctttccag cgccatcgtg ggttggttat
     5581 tggtgcgcta tagctcactg cctggaaata ccggtgcctg gggttcttct ccaggcggcg
     5641 cagcggcaat ggtcgctatg gcgcaagatt acggcgcaga tattcgcctg gtggcgttta
     5701 tgcaatatct gcgagtgctg ttcgtcgcgg gggccgctgt tctggtcacc cgcatgatgc
     5761 tgggtgataa cgctgaagcg gttaaccagc aaatcgtctg gttcccgcca gtgagcatta
     5821 atctcctgct tacgattttg ctcgcagtcg ttgccggtac ggcgggatgc atgctgcgtc
     5881 ttccttccgg cacgatgctc atcccgatgc tggcgggcgc agtgctccag tctggtcagc
     5941 tcatcaccat cgaactaccg gaatggctgc tggcgatggc gtatatggca attggctggc
     6001 ggattggtct tggtttcgat aaacaaatct tactgcgggc actacgcccg ctaccgcaaa
     6061 tcctgctgtc gatttttgct ctgctggcta tttgtgcagg tatggcgtgg ggactgaccc
     6121 ggtttatgca tattgatttt atgactgcct acctcgccac cagtcccggc gggcttgata
     6181 ccgtggcggt catcgccgca gggagcaatg ccgatatggc gcttattatg gcgatgcaaa
     6241 ccctgcgcct gttcagtatt ttgctgacgg ggccagccat tgcgcggttt atttcaacct
     6301 atgcgccgaa gcggtcggcc tagtgctggc agcctggaca ccagtaaaac gggcgcgagg
     6361 acaacgtggt tttctcaatg atgccgccac agcgttcgca cagttcgcca tcacgatgaa
     6421 aaaccttaaa gcgaaacagc gccccatgat gcttattctc atccacctgc ccccgcgtag
     6481 cgtaagaaaa ccgaggaatc tccagtaacg cgtgtgcgag tgcatccagt tgcgccgcat
     6541 tgagatcttt cgctttatga tttccagtca acccaacctg ccagaggatc tccacccgca
     6601 aataattgcc aagcccagcc agaaacgcct gatcgagcag taatcctgca aactgacggt
     6661 tacgaaagcg cggcgacaat aaccgttcct ttaccacctc cggcgtcaga ttcggatcca
     6721 gcacatcggg accaacgcgt tgtaaaaacg gatgcgtggt cagttgttcc ggcctcaaca
     6781 tctcaatatc cgaggcgcta taaagcagaa tggttttgtc agccgtttgc agttttaccc
     6841 gcaatactcg cgtggtctgc ggctcttcgc cggtatcaac cacgcgccag acgccgtaaa
     6901 gctgattatg gctgtagagc gttaaaccgt tgggaaaatg agttaacaac gccttaccac
     6961 gcgtttccac atgggtaacg tgttgaccga taagttgtga ttgataagtt tttaactgcg
     7021 ggaaggcaaa ccagacatca gttagtggtt tgcctttgat cgccgcctcc aggttatccg
     7081 ctgcacggcg gatctccggg ccttcaggca tggtgatatc cttttttcat tctttattaa
     7141 gcggggttat gctgcgacaa caatcccctt tttggcaaat gtagctcgta atcggcgggc
     7201 gaacgccaga gcgtgctcgc cgtcgccatg caggcagacc gtttgcgccg cgaccgttgc
     7261 ccattcgccg gtgatacttt tgactctgcc gtgttgcacc atctccagcg tttgcgccaa
     7321 cgcctgttct tcgttttcaa tcaacgcgcc tggctgactt cgcggcacca gcgagccgtc
     7381 agcctgataa ccgcgatcgg caaacacttc ctcgcgcgtt gtcaggccat attgcttgcc
     7441 tgcacgaatc agctcgcttc ctgccagccc aacgagaacc aatgccggat cgcaagcgta
     7501 taccgctctg gcgatggcgt ctgccagttg tgcttctttc gccgcctggt tgtacaacat
     7561 gccgtgcggt ttgacatgac gcattacgcc gccttgcgca cgggcaatcg ttgccagcgc
     7621 gccaatttga tacagcgtct gggcgtaaac ggtttccggc ggcagctgca tggcgctgcg
     7681 accaaaattt tccctgtcgg gaaaactcgg gtgagcgcca atcgcgacac cattttttat
     7741 tgcttcacgc acgcaagcct gcatggtttg cgcatcgcct gcatgaaatc cacaagcaat
     7801 attggcagag gaaaccagcg ttaatagctc tgcgtcgctg gcgcagcctt cgcccagatc
     7861 ggcgttcagg tcaattttca ttgtgcagcc gccacgctaa ttgttcaaaa taacgttgct
     7921 gatcttgccg cgctttcagt gcctcttcca gtgaacactg gacaaaatga atcggctgac
     7981 cgagcggaat ttgcgccaga tggtacatat cagcctcaat gatacaggca atacgtggat
     8041 aaccaccggt ggtctgtgcg tcgttcatca acacaatcgg ctgcccgtta tgcggcacct
     8101 gcaccacgcc cggtaacaaa ccgtgagata acagttcgcg atcggtgatg cgttttaaaa
     8161 tttgcccctg taaacgatag cccattcggt tactttgcga gctaagctgc cagggcgaac
     8221 gccagaatgc atcctgcgag gcgcgatcga actcatgata ttccggcccc ggcaaggcgc
     8281 gaatgcggtt gccccacagc agctgtttaa cgccctgcgc ttccataaaa tcacgcttcg
     8341 atttgccaat cgggagtcag tcaccatcct tcagtaaacg gccttccagc ccgccaatcc
     8401 ccactttgag atcggtgctg catgacccca ttaccggcgg aacatcaata ccacccgcga
     8461 ccgccagata actgcgcatc ccgtgctgcg ggcgtttaag cgttaaacgc tggcctgctt
     8521 tcatcggcaa tcgccagccg gtccagacgg cgttatcatc caaccgcgct tcgcaacctg
     8581 cacccgtcag ggcaaaccac ccatcagttt cgaactcaac tgttaactga ccgagcgtga
     8641 tctccagcgc gggggcattg gcgtcattac ccaccagcag gttagcaatg cgcagcgcag
     8701 gcatatccag tgcgccacag tggctgatac ccgactggca aaaaccgtga cgaccgccat
     8761 cctgcacagt ggtgtacatg cccgcacgaa taatcttcag catattccct ccttctgcgg
     8821 tacaaatcgc acgctgtctc ccggacgtaa taagatgggt tcgtcacgcg ccggatcaaa
     8881 caggctgagt gaggtatgac caatcaactg ccagccaccc ggcgttgcca gcggataaac
     8941 accagtctgc ggcccgccga tcccgacaga acctgccgga acgagcaagc gcggttcagc
     9001 gcgccgtggc gtgtgtagtt gttccggcaa actcccgaga tacgggaagc ccggttgaaa
     9061 acctaaaaac cagaccacgt attccacgga ggagtgcaat tcaacaacct gtttttcgct
     9121 caacccgcaa tacgccgcga ccaccgccaa atccggtccg cctgcaccac cgtaaaccac
     9181 cggaatttca ataaagcgag actcaggttc cagggcctcg ctctcctccc accagcgttg
     9241 caaacgctct atggcatcca gcgccagcga ctcaggatta cgcaaaatca ccgtgatatt
     9301 gttcatgccg ggaatggctt caaccacatt cggcatatcc accagacgct gcgccagtcg
     9361 ccagatccgt ttctggctag ccagcgtcac cggcggttcc agttccagca ctaccgccgt
     9421 ttcacctatc agataacaac gcgctcgttg cactttcgat ccctctgatt attcgttatg
     9481 caggattagg aatatcaata aaggtcacat caagatcggt attttcattc agccactcgc
     9541 tcaatgcgcg aataccaccg cgttcagtgg catggtgacc tgcagcataa aaatgcaatc
     9601 cctgctcgcg ggctgaatga atggtctgtt cagaaacttc gccagtaata aaagcatcca
     9661 cgccaaaacg cgcggcgcta tcgataaaac tttgcccgcc gcccgtgcac caggcgacgc
     9721 gctggaccac ctcaggtccg gtatcgccac accataacgg cttacgtccc agacgcgctt
     9781 caatccagga ggctagttcc attcccggca caggcatggt cagctctccc cacggcacca
     9841 acggctcaat ttcgcccatg accgtgatcc ccagtaacgc cgccagctgt gcgttattgc
     9901 ccagctcagg atgtgcgtca agcggcaaat gccagccata caggttgata tcattcgcca
     9961 gcaacgtttt taaacggtta cgcttcattc cgcgaatgac cggagactct cctttccaga
    10021 agtagccatg atgcacaatg accgcatccg cgcccagacg cactgcctca tcgagcaaag
    10081 cctggctggc ggtgacaccg gtaacaattt tttgcaccgt ctctttgcct tccacctgca
    10141 aaccattcgg cgcgtaatca ctaatcgccg cgctgttcag tttttcgtta atcagttgtt
    10201 ccagttcggt gtttttcatc ttcgcgcctt ccgtattctg atagctacac cgttaacata
    10261 gcgttacatc gcctgttcgt cgatagccaa tcttttgaca tattaagaaa caaacccgac
    10321 gccaaaagac gcaaattgtt tatatcatca cgcttcagct aatgtatatt tatgcaatca
    10381 gacagcatat tgcataaata atcagatctg tatcaaagtt ttaactttaa ggaaacacaa
    10441 gcacgccgcg caactattac gtgacgttgc cgtcaattgt ggaattatta tgaataaaca
    10501 cgcatcacaa ccgcgcgcta tttactatgt cgttgcgctg caaatctggg aatattttag
    10561 cttttacggc atgcgtgccc tgctgattct ctatctcacc aatcaactaa aatacaacga
    10621 tactcacgcc tacgagttat ttagcgccta ctgttcgctg gtgtatgtca cgccaatcct
    10681 cggtggcttt ttggcggata aagttctcgg caatcgcatg gcggtgatgc tgggggcgtt
    10741 gttgatggcg atcggtcatg tggtgctggg tgccagtgag atccatccgt cattcctcta
    10801 tctgtccctg gcgattatcg tctgcggcta tggcctgttt aaatctaacg taagctgtct
    10861 gctcggcgag ctgtatgagc caaccgatcc gcgtcgtgat ggcggtttct cgctgatgta
    10921 tgcggcgggt aacgtggggt ctattatcgc acctatcgcc tgtggttacg cccaggaaga
    10981 gtacagctgg gcgatgggct ttggcctggc ggcggttgga atgatcgcgg gtctggtcat
    11041 tttcttatgt ggcaatcgtc atttcactca tacccgcggc gttaacaaaa aagtactgcg
    11101 tgcgacaaac tttctcctgc cgaactgggg atggctgctg gttctgctgg tggcaacgcc
    11161 tgcactgatt accgtgctgt tctggaaaga gtggtcggta tacgccttaa ttgtcgcgac
    11221 tattattggc ctgggtgtac tggcaaaaat ttatcgcaaa gcagaaaacc agaaacagcg
    11281 gaaggagctg gggctgattg tgacgctcac cttcttcagt atgttgttct gggccttcgc
    11341 acaacagggc ggtagctcga ttagccttta tatcgaccgc ttcgttaacc gcgatatgtt
    11401 tggttatacc gttccgaccg cgatgttcca gtcgattaat gccttcgcag ttatgctgtg
    11461 cggtgtgttc ctggcgtggg tggttaaaga aagcgtcgca ggtaatcgta ccgtgcgcat
    11521 ctggggaaaa tttgctcttg gccttggcct gatgagcgcc ggattctgca ttctgacctt
    11581 aagcgcccgc tggtccgcaa tgtatggtca ctcttctctg ccactgatgg tgttaggcct
    11641 cgcggtgatg ggctttgcgg aactgtttat cgacccggtt gccatgtcgc aaattacgcg
    11701 cattgaaatt ccgggcgtga ccggcgtatt aaccggcatc tacatgctgc tttctggcgc
    11761 gatcgcgaac tatctggcgg gcgtgattgc cgatcagaca tcgcaggctt cgtttgatgc
    11821 ttccggggcg atcaactact ccatcaatgc atatattgaa gtatttgatc aaattacctg
    11881 gggcgcactg gcgtgtgtag gactggtact gatgatttgg ctgtatcagg cgctgaaatt
    11941 cagaaaccgc gcgctggcgc tggagtcata ataggccagc accgatgcct gatgcgacgc
    12001 ttgacgcgtc ttatcaggcc tacgtatttc ctgcaattta ttgaatttgc acaaatttgt
    12061 aggctggatt aggcgttcac gccgcatccg gcatctgggc tcgattgcct gacgcgtctg
    12121 ttatttcccc ttccgcgccg cctcatacgc tgccaacgtt tgtactctcg cctctttgtg
    12181 ctcgactatc ggttgcggat aatccagctt cacacctgct ttctgcgccc acttccacgg
    12241 ctcatgcacc gctttccctg gtacatcgcg cagctccggt aaccatcgac gaataaactc
    12301 gccctcacgg tcaaatttct cgccctgagt tatcggattg aaaatacgaa aatacggcgc
    12361 agcatcagta cccgtagaag ccgcccactg ccagccaccg ttattagctg ccaaatcacc
    12421 atcaatcagc tgcgacatga aatatcgctc gccttcgcgc cagtcgatca acaaatcttt
    12481 caccagaaaa ctggctgtaa tcatccgtag ccggttatgc atccagccag tgctgttaag
    12541 ctgacgcata gcggcatcaa taatcgggta tcccgttttg cctttctgcc aggcctgtaa
    12601 atgtgcggga ttgctttgcc actgcacacg atccgtccag gcaataaatg gacgatgttt
    12661 acacaacgag gggtaatacg tcatcagatg acggtagaac tcgcgccaga tcagctcact
    12721 aagccagaca ctaccggccc caccgtccag cgcctgcggc tgttccgcca acaagcgatg
    12781 caaacactgg cgaggcgata acccgcccgt tgccaagctg gcggacaaac ggctggtgcc
    12841 ttccactgcc ggaaaatctc gttgttgctc atactctcca gcaccgttct cgcaaaactg
    12901 gcgtaattgc gcaatcgccg ctttttcttc caccgggaaa tgcgcagtat cgaaagactg
    12961 acgaggataa ttcagcgtaa tggatggcgc gggctttatc gatccgctac tacgaacttt
    13021 tggtgcagcg acgcactccg gcatcccttc ccgcagccgt ttcagccagg cattcttaaa
    13081 aggcgtaaag actttgtaca tctcgtgatt gccggtcatc accgcccccg gcggcaggat
    13141 cacgctgtca tcaaatcctt cacacaccac gttacgcagc gctctttcaa cgtccacatc
    13201 ccgcgctcgc tcattcacct catactgata gttataaaaa agatgagtaa cgctgttttc
    13261 cgcgcacacc tgtttaacta tttcgacact ggcgacaaag tcatccactt cacggaacaa
    13321 taaaggaata cctttttccg ccagcgcgat ttgtagccca ttcagttgag cattgataag
    13381 ttcagcctga cgcggcgaca tgttatgcgt ctcccacttg cgtggtgtag cgatatacag
    13441 cgccagcacg cgtgcagacg aattgcggca ggcggcagcc agtgcgagat tatcgtgcag
    13501 acgtaaatcc tggcgaaacc aggccagatg ggtagtcata aaactcctga atggcgcaag
    13561 ttaatcacac gcggccaaag ttcgaaataa cgctgccctg aaagccaggc gtcaggataa
    13621 agcgccatat agtgtttgat acgcatcagc ggcgcaagaa gtggttgctc gccacggcga
    13681 taactgtcga taagcgcagc cagctgctgg cgttctgtgg aatcaatatg cgggcgaaaa
    13741 taaccctgaa cgtgcatcaa aacgttggtg tgatcgcgcg ggttggcggg atgagaaagc
    13801 aaaacaatta cgcgttggcg gtactggttg taaaaatcat ccagattttg ccactgatga
    13861 atatcagcca caaacgggcc aagtttgcgg tactcgggct gagaatgggc cagaaagacg
    13921 agtttatagc ggctgtgata atcgagaagc gccccgcgcg ttaagccatg ttctttcagt
    13981 cgatgtaact catgaagggc gaagatacgg attagggtgc tgtcatcaaa tcgttgtaga
    14041 ttcattactg ctcctgaacg gtatacagga gtaagtgtag gcgttggtaa atgatgtttg
    14101 tgaagttaat ccgctagcag ggcaagatta cgaaagcgcg ctccccgcaa gcaactaatc
    14161 aaggtagtta atcatggctg aacacgatat tacgccagaa gtcactattt ccaaaacaca
    14221 gcgccgctat aaagtcggct atgtcagagc ccgccaccag gaccgcaaaa ccggtatagc
    14281 ccggtattat tcacaacatc ccagtctgca ccttaagggc gactggctga aagaggccgg
    14341 atttgatacc ggacggggtg tcacggtgaa gatttcggag gggggcctga ccatcatcgc
    14401 tgacagtgac gaggtgcagg aactgcggga tgggatgttt agtgtgctga acgaaagtta
    14461 aatattaaaa aagctgggaa gatttaaaac cttcctagct ttttattctg tcttattata
    14521 atggatactc atcatcttcc gatgaattta taa
//
LOCUS       DAZSNM010000096        14538 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZSNM010000096 DAZSNM010000000
VERSION     DAZSNM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14538
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.096"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..267
                     /locus_tag="ACHW42_002399"
     CDS             <1..267
                     /locus_tag="ACHW42_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8944193.1"
                     /translation="KGAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRV
                     DRVKSNIPMQRGGQAEEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(324..1757)
                     /gene="bglA"
                     /locus_tag="ACHW42_002400"
     CDS             complement(324..1757)
                     /gene="bglA"
                     /locus_tag="ACHW42_002400"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR8944194.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            1802..2112
                     /gene="yqfB"
                     /locus_tag="ACHW42_002401"
                     /pseudo
     CDS             1802..2112
                     /gene="yqfB"
                     /locus_tag="ACHW42_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
     gene            2276..2935
                     /locus_tag="ACHW42_002402"
     CDS             2276..2935
                     /locus_tag="ACHW42_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR8944195.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(3131..4111)
                     /gene="ygfZ"
                     /locus_tag="ACHW42_002403"
     CDS             complement(3131..4111)
                     /gene="ygfZ"
                     /locus_tag="ACHW42_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR8944196.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            4354..4620
                     /gene="sdhE"
                     /locus_tag="ACHW42_002404"
     CDS             4354..4620
                     /gene="sdhE"
                     /locus_tag="ACHW42_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR8944197.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            4601..5008
                     /locus_tag="ACHW42_002405"
     CDS             4601..5008
                     /locus_tag="ACHW42_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR8944198.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(5048..5569)
                     /gene="fldB"
                     /locus_tag="ACHW42_002406"
     CDS             complement(5048..5569)
                     /gene="fldB"
                     /locus_tag="ACHW42_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR8944199.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            5681..6577
                     /gene="xerD"
                     /locus_tag="ACHW42_002407"
     CDS             5681..6577
                     /gene="xerD"
                     /locus_tag="ACHW42_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR8944200.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            6602..7312
                     /gene="dsbC"
                     /locus_tag="ACHW42_002408"
     CDS             6602..7312
                     /gene="dsbC"
                     /locus_tag="ACHW42_002408"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR8944201.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            7318..9051
                     /gene="recJ"
                     /locus_tag="ACHW42_002409"
     CDS             7318..9051
                     /gene="recJ"
                     /locus_tag="ACHW42_002409"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR8944202.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            9142..10240
                     /gene="prfB"
                     /locus_tag="ACHW42_002410"
     CDS             join(9142..9216,9218..10240)
                     /gene="prfB"
                     /locus_tag="ACHW42_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR8944203.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            10250..11767
                     /gene="lysS"
                     /locus_tag="ACHW42_002411"
     CDS             10250..11767
                     /gene="lysS"
                     /locus_tag="ACHW42_002411"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR8944204.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(11810..12358)
                     /gene="idi"
                     /locus_tag="ACHW42_002412"
     CDS             complement(11810..12358)
                     /gene="idi"
                     /locus_tag="ACHW42_002412"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR8944205.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            12413..12484
                     /locus_tag="ACHW42_002413"
     CDS             12413..12484
                     /locus_tag="ACHW42_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944206.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(12481..12606)
                     /gene="yqfG"
                     /locus_tag="ACHW42_002414"
     CDS             complement(12481..12606)
                     /gene="yqfG"
                     /locus_tag="ACHW42_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR8944207.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(12608..14056)
                     /gene="uacT"
                     /locus_tag="ACHW42_002415"
     CDS             complement(12608..14056)
                     /gene="uacT"
                     /locus_tag="ACHW42_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFR8944208.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         3642 a         3600 c         3820 g         3476 t
ORIGIN      
        1 aaaggggcga ttgattcgtt aaccaccgga ctatcgctgg aagtcgccgc gcaggggatc
       61 cgcgttaact gcgtgcggcc agggtttatt tataccgaaa tgcacgccag cggcggcgag
      121 cctggacgcg tcgatcgcgt taagtcgaac atccccatgc agcgtggtgg acaggcagaa
      181 gaggtcgcgc aggccattgt ctggctacta agtgataaag cctcttacgt cacgggaagt
      241 tttatcgatt tggcgggcgg gaaataaaac agggaagttg tctgaccgga tgcaacaagt
      301 attgcatccg gtacttcatc gacttaaagc ttctcgccgt tgctggcaat cacctctttg
      361 taccagttaa agctcttctt acgtgaacgc gacatatcgc cagtaccgtc gtcatgttta
      421 ttcacataga taaagccgta gcgtttgctg tactgcccgg tagtgaatga cacgcagtcg
      481 atgcagcccc acggtgtgta gcccatcaga tccacaccat cgtaagtcac tgcttttttc
      541 atctcttcaa tatgggcgcg caggtagtca atgcggtagt cgtcgttgat gctgccatct
      601 tcttccactt tgtcgtaagc gccaaaaccg ttttcgacaa taaacagcgg cctctgataa
      661 cgctcataca gttcgcaaag ggcatagcgc agacctactg gatcaatctg ccagccccag
      721 tcagacgctt taacatacgg gttcggtacg ctgccttcaa aaccagagat cgcatcgccg
      781 gtgccgcctt cggccttcac tgcgttggtc atgtaatagc tgaaaccaag ataatcgcag
      841 gtgccttcgc gcagtacatc gagatcgccg tcttccattt tgatattaaa tccgcgacgt
      901 tcccactcgt tcaatacata ggacgggtaa tagccgcata gctgcacatc ggtaaagacg
      961 tagcgttcgc gcatcgactc ctgagcgaac atcacatcgt ccgggttaca tgagtaggga
     1021 tagagcggca ccatcgccag catacagccg actttcatct ccgggttaat gcgacgcgcg
     1081 gctttcactg ccagggcgct ggcgacaaac tggtgatgca gcacctggta catcgtctct
     1141 tccgggttgt catgctcggt atacaccacg ccggagcagc agtaaccaaa cagcggcgca
     1201 cgccagttac gctggttgtt tatttcgttg aaggtcatcc agtatttgac tttatgcttg
     1261 tagcgttcga ataccacttc cgcgaaacgg acaaagaaat caacgaattt acggttggtc
     1321 cagctaccgt attgctgcac cagatgcagc ggcatttcaa agtgggagag ggttatcacc
     1381 ggttcgatgt tgtatttcag cagttcatcg aacagagagt cgtaaaactt cagcccttct
     1441 tcgtttggct gagcttcatc accttttgga aaaatacgcg tccaggcgat ggaagtccga
     1501 aaacatttga agcccatttc ggcaaatagc ttgatgtctt ccttgtagtg accataaaaa
     1561 tcaacggctt catggtttgg atagtatttt cctggcaaga cttctttggt gatttcgcgc
     1621 ggcacgccgt gtgcgccaca ggtcagaacg tcacaaatgc tcggtccttt gccgcctttg
     1681 ttccagccgc cttcgacctg atgagcggca acggcgccac cccataagaa atctttcggt
     1741 aaggtgagtt ttttcactat cataggctcc agatcagtca attcttacag gaaatagtac
     1801 catgcagcca aacgacatca cttttttcaa cgtttccagg atgacattct ggctgggcgt
     1861 aaaaccatca ccatccgcga cgagtctgaa tcgcacttca aaacgggtga tgtgcttcgt
     1921 gtcggacgtt ttgaagatga cggttatttt tgcacgattg aagtcaccgc aacctcaacc
     1981 gtaacgctgg atacactgac ggaaaaacat gcagagcagg aaaatatgac cctgcctgaa
     2041 ctgaaaaagg tcattgctga catctatccc ggtcagacac aattttatat gattgaattt
     2101 aaatgtcttt aaattgaact cacgcctgtt agttgaaatt tcgcatttaa tttcatgatt
     2161 tttaggagat tagtctcaaa tcagaattta ttttagctaa caggtgttca ctggaactat
     2221 tctcagttac gctggagagg tatacatgtg cgtgtacgtt tctctggagt aagttatggt
     2281 tcagaagccc ctcattaagc agggatattc gctggcagag gaaattgcca acagcgtcag
     2341 tcacggcatt gggctggtgt ttggtatcgt tgggctggtg ttgctactgg ttcaggcggt
     2401 ggatctcaat gccagcgcca cagcgataac cagttacagc ctctatggcg gcagtatgat
     2461 cctgctgttc ctcgcttcga cgctctatca cgccattcct catcaacggg caaaaatgtg
     2521 gctgaagaaa tttgaccatt gcgctattta cctgttgatt gccggaacct acacgccgtt
     2581 tttgctggtg gggctggatt ctccgttagc gcgcgggttg atgattgtta tctggagcct
     2641 agcattgctg gggattctgt tcaaactgac catcgcgcac cgattcaaaa ttttatctct
     2701 ggtgacttat ctggcgatgg gctggctgtc gctggtggta atttatgaaa tggcagttaa
     2761 gctcgcggcg ggcagcgtta ccttactggc ggtaggcggc gtggtttatt cgctcggggt
     2821 gattttctac gtctgcaaac gcattccata caaccatgcc atctggcacg gcttcgtgct
     2881 cggcggtagt gtgtgccact ttctggcgat ctatttgtat attgggcagg cgtaatgggt
     2941 atgcctttgt aggcctgata agacgcgtca gcgtcgcatc aggcatcgga gcgcaaccgc
     3001 cggatgcggc gtgaacgcct tatccgatct acgggcctcg tccctgttgt gataaaggca
     3061 tcaaatccca gacatgataa gacgcgtcag cgtcgcatca ggcgataaat gcctgatgcg
     3121 atacagagat ttactcttcg agcgaatacg gcagcggctc gatatgcagt gtattcgcat
     3181 cgtcgcgtac gcggaagatg ctgtccggtt ccatatcgtt attcatgacg acctgtacca
     3241 cgacctgacc atcttccagt tttaccgcag ccagcaccgt accggtacga cgccagttct
     3301 cgcccatttt cagctctaag tcttcaccag cttccggcag tcggctggca ctacctgcca
     3361 gcaaccagag cgcgcgtttg ttggcaccac ggaattttgc tcgcgccacc atctcttgtc
     3421 cggtataaca gcctttctta aagctgatac cgcccagcgc ctggagattg gtcgcctgtg
     3481 ggataaactg cccgctgttg gcggcatcaa tcaccgggaa accagcttca atgtttaatg
     3541 ccagccactg ttggctattg ttcagttccg cttcaccgcg cagtttatcg gtcagcatat
     3601 tagcagtagc ttcatcggtt acgatcagga aacgttctgc cgggtgttca aaccatagca
     3661 aagtggtcgc gccttctttg actacctgtt tttctttcga aggcagttcg ctaaagagat
     3721 ttgccagcgc ggcgcgcgcc tgaaaaccgg caacaccaag cagcacacgc tcgtcgtctg
     3781 gcgcgatggt cactttagag aataccgcat attttttcag ttcagtcagc tgcggttcac
     3841 gcacgctgcg ccgttcaatc catgcaaagc catcgccgtc gcggaacaga cgtaaattgc
     3901 tccacatttt acctttggcg tcgcaatggg cggcgagcag gtgctgatct tctgccatct
     3961 ggctgacatc tgctgtcacc tgaccctgca tatatttttc gctgtccgcg ccagtaatgg
     4021 tggcaagcgc ccagtcatca agcgtcatca gcgtcagcgg taaacgggca gaagccgtag
     4081 gctgacgtgg aggaaaaggt gtaaaagcca taatagagtc ctgagttgct taacggcgta
     4141 ttaatgacta atggtaaaag agctatgagg caatgcaagt gctttcacta cgcgatctgt
     4201 gccttctgcg aggcgactgc caaaaaaatg aagcccttga attgttgtga attaatttcg
     4261 cgatcgcgat ggtgtttact gatattcccg taaaagaagt cgaaaaacac gttacaatag
     4321 ccgggtactg gattttcgtg agacaggaag aagatggaca ttaacaacaa agcccgaatt
     4381 cattgggcat gccgccgtgg tatgcgcgaa ctcgatattt caatcatgtc gtttttcgaa
     4441 catgagtacg acagcttaag cgatgacgaa aaacgcatct ttattcgtct gctggaatgt
     4501 gacgatccgg acctgtttaa ctggctgatg aatcacggta aaccggccga tgcagaactg
     4561 gaaataatgg tccgactcat ccagacacgg aaccgggaac gtggtcctgt ggcaatctga
     4621 tttgcgcgtc tcctggcgcg cacagtggct ttccttgctg attcatgggc tggttgccgc
     4681 tgttatttta ctcatgccct ggccgctcag ttacaccccg ttatggatgg tgttactttc
     4741 gctggtggta tttgattgcg ttcgcagcca gcggtgtatt aatgctcgcc agggggaaat
     4801 tcgcttgttg atggacgggc gtttgcgttg gcaagggcag gagtggagca tcgtcaaagc
     4861 accgtggatg attaagagcg gcatgatgct gcgtttacgt tctgatggcg gtaaacggca
     4921 acatttatgg ctggcagccg acagcatgga cgaagccgaa tggcgggatt tacggcggat
     4981 tttgttgcaa caagagacgc aaagataaag atgacggcaa agcatatgcg ctaagccgtc
     5041 agtgagatca ggcgtaatgc tctgccattt cgttgaggat ttgctcgcac cagctctgaa
     5101 tacgctcgtc gctaaggtca tactggttag tttcatccag cgccagaccc acgaacagtt
     5161 gcccgtcagc aatcaccggt ttcgggctgg taaattcata tccttccgtt ggccagtagc
     5221 cgacgaactt cacgcctttg gtcgagagtt tgtcatgcag cataccgagc gcatcgagga
     5281 accactcgcc gtatcccagt tgatcgccaa gcccatacag cgcaataatt ttaccttcaa
     5341 ggttcaggtc gtcgagctga tcccagacgg cttcccagtc ttcctggatt tcaccaaaat
     5401 cccaggtcgg gatacccaga atgagcacat cgtactgctc cattaatttc ggggagtcgt
     5461 ccttgaggtt atgtaaggtc accagttctg ggccgataat atcgcggatt ttttctgccg
     5521 ccatttcggt gtaacaggtg ctggaaccgt aaaaaagacc catattcata acgtaagctt
     5581 ctcgattctg ctgtggcttt tgcagctagt gtatcagaat cgctttagtc acggcacaat
     5641 aaggcataat ggatcaaatg agtgaccata aggggcgcaa gtgaaacagg agctggcacg
     5701 catcgagcag tttcttgatg ctctgtggct ggaaaaaaat ctggctgaaa atacgttgaa
     5761 cgcttaccgt cgcgatctgt caatgatggt ggagtggttg catcaccgcg ggttgacgct
     5821 ggcgacggcg caaagtgacg atttgcaggc attactggca gaacggctgg agggcgggta
     5881 taaagccacc agctcagcgc gtttgctgag tgcagtgcgc cgattgttcc agtatcttta
     5941 tcgcgaaaag tttcgtgaag acgatcccag tgcgcatctc gcttcaccga aattgcccca
     6001 gcgtttgcca aaagatttaa gcgaagcgca ggtcgaacgt ttattacagg cgccattaat
     6061 tgatcagcca ctggagctac gcgataaagc catgcttgaa gtgttgtatg ctaccgggct
     6121 gcgtgtctct gaactggtcg ggctgacaat gagtgatatc agcctgcgtc agggcgtagt
     6181 gcgggtcatt ggtaaaggca acaaagagcg cctggtgccg ttaggggaag aggcggttta
     6241 ctggctggaa acctatctgg aacatgggcg tccgtggctg ctgaatggtg tgtcaattga
     6301 cgtgttgttt cccagccagc gtgcgcagca gatgacgcga cagaccttct ggcaccgcat
     6361 taaacattat gccgtgctgg cgggtatcga cagcgaaaag ctgtcaccgc atgtgttgcg
     6421 tcacgctttt gccactcatt tattaaatca tggtgcggat ttacgcgtgg tgcagatgct
     6481 actgggccac agcgatcttt ccaccacgca aatttatacg catgtcgcta ccgagcgtct
     6541 gcggcaactt catcaacagc atcacccgcg ggcgtgatgt ctgaaaagaa cgggaagatt
     6601 tatgaagaaa ggttttatgt tgtttacttt gttagcggcg ttttcaggct ttgctcaggc
     6661 tgatgacgcg gcaattcaac aaacgttagc caaaatgggc atcaaaagca gcgatattca
     6721 gcccgcgccc gtagctggca tgaagacagt tctgactaac agcggtgtgt tgtacatcac
     6781 cgatgatggt aaacatatca ttcaggggcc aatgtatgac gttagtggca cggctccggt
     6841 caatgtcacc aataagatgc tgttaaagca gttgaatgcg cttgaaaaag agatgatcgt
     6901 ttataaagcg ccgcaggaaa aacacgtcat caccgtgttt actgatatta cctgtggtta
     6961 ctgccacaaa ctgcatgagc aaatggcaga ctataacgcg ctggggatca ccgtgcgtta
     7021 tcttgctttc ccgcgccagg ggctggacag cgatgcagag aaagaaatga aagctatctg
     7081 gtgtgcgaaa gataaaaaca aagcgtttga tgatgtgatg gcaggtaaaa gcgtcgcacc
     7141 agccagctgc gacgtggata ttgccgacca ttacgcactt ggcgtccagc ttggcgttag
     7201 cggtactccg gcagttgtgc tgagcaatgg cacacttgtt ccgggttacc agccgccgaa
     7261 agacatgaaa gaattcctcg acgaacacca aaaaatgacc agcggtaaat aattcgcgtg
     7321 aaacaacaga tacaacttcg tcgccgtgaa gtcgatgaaa cggcagactt gcccgctgaa
     7381 ttgcctccct tgctgcgccg tttatacgcc agccggggag tacgcagtgc gcaagaactg
     7441 gaacgcagtg ttaaaggtat gttgccctgg cagcaactaa gcggtgtcga aaaggccgtt
     7501 gagatccttt acaacgcttt tcgcgaagga acgcggatta ttgtggtcgg tgatttcgac
     7561 gccgacggcg cgaccagcac ggctctaagc gtgctggcga tgcgctcgct tggttgcagc
     7621 aatatcgact acctggtacc aaaccgtttc gaagacggtt acggcttaag cccggaagtg
     7681 gtcgatcagg cccatgcccg tggcgcgcag ttaattgtca cggtggataa cggtatttcc
     7741 tcccatgcgg gagttgagca cgctcgctcg ttgggcatcc cggttattgt taccgatcac
     7801 catttgccgg gcgacacatt acccgcagcg gaagcgatca ttaaccctaa cttgcgcgac
     7861 tgtaatttcc cgtcgaaatc actggcaggc gtgggtgtgg cgttttatct gatgctggcg
     7921 ctgcgcacct ttttgcgcga tcagggctgg tttgatgagc gcggcatcgc aattcctaac
     7981 ctggcagaac tgctggatct ggtagcactg gggacagtag cggacgtcgt gccgctggac
     8041 gctaataatc gcattctgac ctggcagggg atgagtcgca tccgtgccgg aaagtgccgt
     8101 ccagggatta aagcgctgct ggaagtggca aatcgcgatg cacaaaaact agccgccagc
     8161 gatttaggtt ttgcgctggg accgcgtctc aatgctgccg ggcgactgga tgatatgtcc
     8221 gttggtgtgg cgctgttgct gtgcgacaac atcggcgaag cgcgcgtgct ggcaaatgaa
     8281 ctcgatgcgc taaaccagac gcgaaaagag atcgaacaag gaatgcaggt tgaagccctg
     8341 accctgtgcg agaaactgga gcgcagccgt gacacgctac ccggcgggct ggcaatgtat
     8401 caccccgaat ggcatcaggg cgttgtcggt attctggctt cgcgtatcaa agagcgtttt
     8461 caccgtccgg ttatcgcctt tgcgccagca ggtgacggta cgctgaaagg ttccggtcgc
     8521 tccattcagg ggctgcatat gcgtgatgcg ctggagcgat tagacacact ctaccccggc
     8581 atgatgctca agtttggcgg tcatgcgatg gcggcgggtt tgtcgctgga agaggataaa
     8641 ttcgaactct ttcaacaacg gtttggcgaa ctggttactg agtggctggc cccttcgcta
     8701 ttgcaaggcg aagtggtgtc agacggtccg ttaagcccgg ccgaaatgac catggaagtg
     8761 gcgcagctgc tgcgcgatgc tggcccgtgg gggcagatgt tcccggagcc gctgtttgac
     8821 ggtcatttcc gtctgctgca acagcggctg gtgggcgaac gtcatttgaa ggtgatggtc
     8881 gaaccggtcg gcggcggtcc actgctggat ggtattgctt ttaatgtcga taccgctctc
     8941 tggccggata acggcgtgcg cgaagtgcaa ctggcttata agctcgatat caacgagttt
     9001 cgcggcaacc gcagcctgca aattatcatc gacaatatct ggccaattta gcgtcatctt
     9061 ctctataaaa aagagcgtgg attgggtaca atcccgctct tatcaccgca ttttgactag
     9121 ctcaataaaa gaaatcagac catgtttgaa attaatccgg taaataatcg cattcaggac
     9181 ctcacggaac gctccgacgt tcttaggggg tatctttgac tacgacgcca agaaagagcg
     9241 tctggaagaa gtaaacgccg agctggaaca gccggatgtc tggaacgaac ccgaacgcgc
     9301 acaggcgctg ggtaaagagc gttcctccct cgaagccgtt gtcgacaccc tcgaccaaat
     9361 gaaacagggg ctggaagatg tttctggtct gctggaactg gctgtagaag ctgacgacga
     9421 agaaaccttt aacgaagccg ttgctgaact cgacgccctg gaagaaaaac tggcgcagct
     9481 tgagttccgc cgtatgttct ctggcgaata tgacagcgcc gactgttacc tcgatattca
     9541 ggcggggtct ggcggtacgg aagcacagga ctgggcgagc atgcttgagc gtatgtatct
     9601 gcgctgggca gaatcgcgtg gtttcaaaac tgaaatcatc gaagagtcgg aaggtgaagt
     9661 ggcgggtatt aaatccgtga cgatcaaaat ctccggcgat tacgcttacg gctggctgcg
     9721 tacagaaact ggcgttcacc gcctggtgcg taaaagcccg tttgactccg gcggtcgtcg
     9781 ccacacgtcg ttcagctctg cgtttgttta tccggaagtt gatgatgata ttgatatcga
     9841 aatcaacccg gcggatctgc gcattgacgt ttatcgcgcg tccggcgcgg gcggtcagca
     9901 cgttaaccgt accgaatctg cggtgcgtat tacccacatc ccgaccggga tcgtgaccca
     9961 gtgccagaac gaccgttccc agcacaagaa caaagatcag gccatgaagc agatgaaagc
    10021 gaagctttat gaactggaga tgcagaagaa aaatgccgag aaacaggcga tggaagataa
    10081 caaatctgac atcggctggg gcagccagat tcgttcttat gtccttgatg actcccgcat
    10141 taaagatctg cgcaccgggg tagaaacccg caacacgcag gccgtgctgg acggcagcct
    10201 ggatcaattt atcgaagcaa gtttgaaagc agggttatga ggaaccaaca tgtctgaaca
    10261 acacgcacag ggcgctgacg cggtagtcga tcttaacaat gaactgaaaa cgcgtcgtga
    10321 gaagctggcg aacctgcgtg agcagggaat tgccttcccg aacgatttcc gtcgcgatca
    10381 tacctctgac caattgcacg cagaattcga cggtaaagag aacgaagaac tggaagcgct
    10441 gaacatcgaa gtcgccgttg ctggccgcat gatgacccgc cgtattatgg gtaaagcgtc
    10501 tttcgttacc ctgcaggatg tgggtggccg cattcagctg tacgttgccc gtgacgatct
    10561 gccggaaggc gtttacaacg agcagttcaa aaaatgggac ctcggcgaca tcctcggcgc
    10621 gaaaggtaaa ctgttcaaaa ccaaaaccgg cgaactgtct atccactgta ccgagctgcg
    10681 tctgctgacc aaagcactgc gtccgctgcc ggataaattc cacggcttgc aggatcagga
    10741 agcgcgctat cgtcagcgtt atctggatct catctctaac gatgaatccc gtaacacctt
    10801 taaagtacgc tcgcagatcc tctctggtat tcgccagttc atggtgaacc gcggctttat
    10861 ggaagttgaa acgccgatga tgcaggtgat cccaggcggt gccgctgcgc gtccgtttat
    10921 cacccaccat aacgcgctgg atctcgacat gtacctgcgt atcgcgccgg aactgtacct
    10981 caagcgtctg gtggtcggtg gcttcgagcg tgtattcgaa atcaaccgta acttccgtaa
    11041 cgaaggtatt tccgtacgtc ataacccaga gttcaccatg atggaactct acatggctta
    11101 cgcagattac aaagatctga tcgagctgac cgaatcgctg ttccgtactc tggcacagga
    11161 tattctcggt aagacggaag tgacctacgg cgacgtgacg ctggacttcg gtaaaccgtt
    11221 cgaaaaactg accatgcgtg aagcgatcaa gaaatatcgc ccggaaaccg acatggcgga
    11281 tctggataac ttcgactctg cgaaagcgat tgctgaatct atcggtatcc acgttgagaa
    11341 gagctggggt ctgggccgta tcgttaccga gatcttcgaa gaagtggcag aagcacatct
    11401 gattcagccg accttcatta ctgaatatcc ggcagaagtt tctccgctgg cgcgtcgtaa
    11461 cgacattaac ccggaaatca cagaccgctt tgagttcttc atcggtggtc gtgaaatcgg
    11521 taacggcttt agcgagctga atgacgcgga agatcaggcg caacgcttcc tggatcaggt
    11581 tgccgcgaaa gacgcaggtg acgacgaagc gatgttctac gatgaagatt acgttaccgc
    11641 actggaacat ggcttaccgc cgacagcagg tctggggatt ggcatcgacc gtatggtaat
    11701 gctgttcacc aacagccata ccatccgcga cgttattctg ttcccggcga tgcgtccggt
    11761 gaaataagca ttacgttatg ctcacaaccc cggcaaatgt cggggttttt tatttaagct
    11821 gggtaaatgc agataatcgt attctggctt cgcgatttgt cgcctgcatc accatccacg
    11881 gactgaacgc ccacggcgtg gcatcaatac cgcgtaatac atcagctaaa tcacaccatt
    11941 gataatccat cacttcatca tcgttgatct gtaacgcact ggtggtgcgt gcggcaaata
    12001 ccggacacac ttcattttcc acaatgcctc tcggatcggt ggcgcggtag cgaaagtcag
    12061 gatagataga ttcaggaggc gtaatttcca cgccaagctc ataacggcaa cggcggatca
    12121 ccgcgtcttc gctgctttct cccagttgtg ggtgcccaca aaccgagtta gtccacacgc
    12181 caggccacgc ttttttgctc agtgcgcggc gggtaactaa taattgcccc ttggcattaa
    12241 acagccaact ggagaacgcg agatgtaaga gggtgtctgc cgtgtgtgcg gcatactttt
    12301 ccagcgtacc cgtgggaact ccctgtgcat tcaataaaat gacgtgttcc gtttgcataa
    12361 tttctcacat gtaattctga tcactcatca ttatagataa ttgaagagtg aaatgattaa
    12421 ccaagagagc gtttatcgac aaccgcccgt tttgagcgga tgccgacagg taaaaaccat
    12481 ttaaaaaagt agcatcatat atttgaagcg actttcaatg gccattgcaa cacagtcaga
    12541 aatgacagga atagagagag taaaaagcaa caacagactg aatatagcgc gcattaaaaa
    12601 attcatatta ttctccatgc tcatttttct tcagcttgcg catcagaagc cacatacgta
    12661 ctgtcctgac ttctaaatct ttatcagaga cggattcctt caccaggtca gcatgatgct
    12721 gatagccatt aaagaagacg ttcagcacaa cggcgctaag ggttgcgagc ataatgccgc
    12781 tatgtagcag cggttgcagt acggccggta acttagaaaa gaaatcgtga gagagcgtcg
    12841 gagtcatgcc aacgccgaga ctgatcgcca caatatagag gttgtaacgg ttggtggtgt
    12901 agttacagcg cgacagaatt cgaatccctg tcgccagtac catgccgaac atcactagac
    12961 cagcgccgcc cagcacaaat tgcggaatgg aggctaccag caccgccatt tttggcacca
    13021 tgccaaacag gattaatata attcccgaag aaatacacac ccagcggcta tgaacgcgca
    13081 tcacgctaac caggccaacg ttttgagaaa atgacgtgtg ggggaagctg ttaaacgtgc
    13141 cgcctatcat tgtccctacg ccatcgacac gcagcccgca aataatatcg tgtgaagaga
    13201 gtttacgacc gactatttca cccagtgcca ggaacatccc catcgactcg ataaacacga
    13261 tgattaacac ggcagtcatg gtcagaatgg aaacgggatc gaaaatcggc ataccaaacg
    13321 acatcggggt gacaatcgca aaccacgagg catcatgtag cccggataaa ttgacttcat
    13381 tcatcatcca cgaaagtaaa aagccaaata caatccccag taatacggcg acgttggaca
    13441 taaatccttt cgcatagcga gtaatgagca agataaaaat taagacggca aaggagatac
    13501 ctaaataaac gggattacca tattgcggat tccctttacc tccagcggcc cagtcaatac
    13561 ccacctgaat gatgctaagc ccaatagaag taataaccac accggtaacc agtggcggga
    13621 ataaaggcat caagcgacca ataagtggcg ctaataatgt ggtgataaaa cccgcggcga
    13681 tagtggcacc aaatatcccc agcaggccga tatccgggtt catcccaatg gctatcattg
    13741 gtgttacagc agcaaaggtc accgacataa tcaccggcag gcggatcccc ataaagcggc
    13801 cgataccgat acattgcaat aatgtgacga tcccgcagca aaagagatcc gagctaatga
    13861 gcatcgcaat agcatctttt gagaggccca gtcggtcacc aatcataaga ggaacagcga
    13921 ctgcacctgc gtacatgaca aggacgtgtt gcagaccgag tatgatcagc tttcctggtg
    13981 ataatatgcg gtcaacctca tcagttgggc ggtcttgccc agaagatgag ggaagttggg
    14041 aatctatggc gctcatcagt gtttctcctt tcgcccgcca gaataaaaag caaccgttta
    14101 cgttaatgaa actgtctaaa cacatgctcg tggagtgcca gtttttgcga ggaaggggag
    14161 ttagagacag gaattgcgga ggcaatcaca taatcgtgct taaaccacga aaatgacaag
    14221 agaaactgac aatggaatta tctgtaattt tctcatattg atatttcatt aatcattctg
    14281 gcatgtcgca ctctcgcatt taatcgtttt tatctggata gcgctctttt gatcggcaat
    14341 gttgaaataa tgaaagcggc atagattgag gggcaataat ttgaactcag tcgcaaaatt
    14401 ataattaaaa cggtcatgct acctaatata tgcccgttta ttgcatcgcg aaatttgaga
    14461 agacataact cattaaatga aagggatgca aatgaatacg tttatcgctg ctgaagctgc
    14521 ggaatgtata ggctgtca
//
LOCUS       DAZSNM010000097        14491 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZSNM010000097 DAZSNM010000000
VERSION     DAZSNM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14491
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.097"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..116
                     /locus_tag="ACHW42_002416"
     CDS             <1..116
                     /locus_tag="ACHW42_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944209.1"
                     /translation="TISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(113..379)
                     /locus_tag="ACHW42_002417"
     CDS             complement(113..379)
                     /locus_tag="ACHW42_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR8944210.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="ACHW42_002418"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="ACHW42_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR8944211.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="ACHW42_002419"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="ACHW42_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="ACHW42_002420"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="ACHW42_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR8944212.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="ACHW42_002421"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="ACHW42_002421"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR8944213.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="ACHW42_002422"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="ACHW42_002422"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR8944214.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(8885..9058)
                     /gene="ynaL"
                     /locus_tag="ACHW42_002423"
     CDS             complement(8885..9058)
                     /gene="ynaL"
                     /locus_tag="ACHW42_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR8944215.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="ACHW42_002424"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="ACHW42_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR8944216.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(10330..10377)
                     /gene="ydaN"
                     /locus_tag="ACHW42_002425"
     CDS             complement(10330..10377)
                     /gene="ydaN"
                     /locus_tag="ACHW42_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR8944217.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="ACHW42_002426"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="ACHW42_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="ACHW42_002427"
                     /pseudo
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="ACHW42_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12641..14107)
                     /locus_tag="ACHW42_002428"
     CDS             complement(12641..14107)
                     /locus_tag="ACHW42_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFR8944218.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGIACGLAWNIQI
                     MILFRALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLT
                     DNLDWRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYT
                     LEEGARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFS
                     FSGGVGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRW
                     LLMAGLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKER
                     LKLASGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSAL
                     FFNRSRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14104..>14491)
                     /locus_tag="ACHW42_002429"
     CDS             complement(14104..>14491)
                     /locus_tag="ACHW42_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR8944219.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3863 a         3656 c         3419 g         3553 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaacttctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt tttgtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatctggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt ttttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccactgtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgcta
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgattccaca cgcgatactc attaaagtaa
    13861 atattcccgc ggatagagta aaaagccaac gagtggaaaa tacccgtgac agccacccag
    13921 agagcggaat aacaataatc tcagcaatta aatagctggt ctgaagccag gccatttcgt
    13981 ctttacctgc ggacagacca ccacctattt catttaggga tgacgataca atttgaatat
    14041 caagctgagc aataaataat cccatacaca tactggcgaa agcaatatgt ttacgcatag
    14101 acatcatctc tcatcaacac tgacggtgac ggacagtccc gggcgaagaa gttgctttaa
    14161 ttcctttgcg tcgtcaaatg caatccgtac cgggacacgc tgaacaattt tggtgaaatt
    14221 gccagtggca ttttctatcg gaatcaagct gaaactggct cctgtcgctg gggaaaggct
    14281 ttcaatgtga ccgtgaaaca cttccccttt gagaatatca gcacggatct cagccttcat
    14341 tcctggcttc attcccgcta tttggttttc tttataattt gcatcaaccc acaactcact
    14401 gactggtact agtgaaacta acgatgtgcc gccttccacc caactgcctg tatgggcgct
    14461 gcggtttgcg acaatgccat caatcggcgc a
//
LOCUS       DAZSNM010000098        14421 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZSNM010000098 DAZSNM010000000
VERSION     DAZSNM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14421
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.098"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..132)
                     /locus_tag="ACHW42_002430"
     CDS             complement(<1..132)
                     /locus_tag="ACHW42_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR8944220.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
     gene            315..458
                     /locus_tag="ACHW42_002431"
     CDS             315..458
                     /locus_tag="ACHW42_002431"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944221.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(677..2215)
                     /gene="aldB"
                     /locus_tag="ACHW42_002432"
     CDS             complement(677..2215)
                     /gene="aldB"
                     /locus_tag="ACHW42_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFR8944222.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="ACHW42_002433"
     CDS             complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="ACHW42_002433"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFR8944223.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3721..5565)
                     /gene="selB"
                     /locus_tag="ACHW42_002434"
     CDS             complement(3721..5565)
                     /gene="selB"
                     /locus_tag="ACHW42_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR8944224.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5562..6953)
                     /gene="selA"
                     /locus_tag="ACHW42_002435"
     CDS             complement(5562..6953)
                     /gene="selA"
                     /locus_tag="ACHW42_002435"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR8944225.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7051..7659)
                     /locus_tag="ACHW42_002436"
     CDS             complement(7051..7659)
                     /locus_tag="ACHW42_002436"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR8944226.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7730..8815)
                     /locus_tag="ACHW42_002437"
     CDS             complement(7730..8815)
                     /locus_tag="ACHW42_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR8944227.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8818..9180)
                     /locus_tag="ACHW42_002438"
     CDS             complement(8818..9180)
                     /locus_tag="ACHW42_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR8944228.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9717..11630
                     /gene="mtlA"
                     /locus_tag="ACHW42_002439"
     CDS             9717..11630
                     /gene="mtlA"
                     /locus_tag="ACHW42_002439"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR8944229.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11725..12873
                     /gene="mtlD"
                     /locus_tag="ACHW42_002440"
     CDS             11725..12873
                     /gene="mtlD"
                     /locus_tag="ACHW42_002440"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR8944230.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12873..13460
                     /gene="mtlR"
                     /locus_tag="ACHW42_002441"
     CDS             12873..13460
                     /gene="mtlR"
                     /locus_tag="ACHW42_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR8944231.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13472..13681)
                     /gene="yibT"
                     /locus_tag="ACHW42_002442"
     CDS             complement(13472..13681)
                     /gene="yibT"
                     /locus_tag="ACHW42_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR8944232.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13965..14327
                     /locus_tag="ACHW42_002443"
     CDS             13965..14327
                     /locus_tag="ACHW42_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR8944233.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3524 a         3840 c         3624 g         3433 t
ORIGIN      
        1 aatggcgtcg gcatgaggat gattacgttt tttagcaatc aggtagggaa tatcatgtaa
       61 aataataatc ccatagaata tcaccagaaa tacaaaaata agcactccca gtgcaaaata
      121 gtccaggaac atatttccct caaagaatat aaaaaagaac aattaacgca tattatgaaa
      181 tgccatgatg cagtgcaatt ttcttattta tataacgaca tattgttttt tatatacttt
      241 ctgaaatgtg ctgtctggtt tttcaacagg acgaggttta tcatattgaa atagtggaac
      301 tattggccaa actaatgaat aactccagta aaacatccac agtacagatt aagcgtatta
      361 aaccttcaat tatctaccgt ttattgctga ttggcctcgg atcaccaatg gtgatttacg
      421 gcctggttcg cccgctcacc atcgaaacgc gagattaaac tcctgacaaa acacgcccca
      481 gaaacgcact tctgttggcg tagtctgggt tattgcgcac gtaggtttaa aacgtaaatt
      541 gtaacaacgt gcgctttgtt tatgccggat gcggcgtgta cgccttatcc ggcctacggt
      601 caggttcccg taggcatgat aagacgcgta agcgtcgcat caggcaatga atacccaatg
      661 cgaccagctt cttatatcag aacagcccca acggtttatc cgagtagctc accagcaggc
      721 acttggtttg ctggtaatgc tccagcatca tcttgtgggt ttcgcgaccg atacctgatt
      781 gtttgtagcc accaaacgca gcatgtgccg ggtaagcgtg ataacagttg gtccacacgc
      841 gcccagcctg tatgccgcgc cccatcttat aggccagatt accgttgcgg ctccagacgc
      901 ccgcgcccag gccatattgc gtatcgttcg ccagctccag cgcttcttcc atcgttttga
      961 aggtggtcac cgccagcacc gggccaaaaa tctcctcctg gaacacccgc atattgttct
     1021 gaccaaacag aatcgtcggt tcgaggtagt agccgtcttt cagttcacct tccagcagct
     1081 tgcgccgccc gcctgtgagc acgtcagcgc cctctttttt accgatatca atgtagttga
     1141 ggatggtttc cagttgcccg tgagaaacct gcgcgcccat ttgcgtcacg ctgtcgagcg
     1201 ggttaccgct gcgaatgctt tcgacacggc ggatggcgcg ttccataaag cgttcgtaga
     1261 ttgactcctg cactaaggca cgactcggac aggtgcaaac ttcgccctgg ttaaaggcaa
     1321 acagtgcaaa gccttccagc gctttatcga aaaaggcatc ttcttcatcc atcacatcag
     1381 caaagaagat atttggcgac ttaccgccca actccagcgt caccggaata atgttttgcg
     1441 ttgcgtattg cataatttgt tggcccactt ccgttgagcc ggtaaacgcc actttggcga
     1501 tgcgtttcga ggtcgccaga tattcgccaa tttccccacc tgcgccattg accacgttca
     1561 ccacgcccgg cggcagtaaa tcaccgacaa tttccattag cagcagtaca gaaagcgggg
     1621 taagacgtgc gggtttcagc accacacagt tgcccgccgc cagcgcggga gccattttcc
     1681 agctcgccat cagcagcggg aagttccacg ggataatctg ccccaccacg cctaacggtt
     1741 catggaaatg ataggccacg gtttcgctat caacttcact gatcccacct tcctgcgccc
     1801 gaatacacga ggcgaaatag cggaaatggt caatcgccag cggtacatcc gcagcactgg
     1861 tttcgcgaat gggtttgccg ttatcccagg tttcagctgt cgctaacagc tcgaggtttt
     1921 gttccattcg atcggcaatc ttaaacagaa tcgccgcgcg atcctgcacc gaggtgtgcg
     1981 cccatttatc tttcactttg tgcgcagcat ccagcgccag atcgatgtct cgtttgcccg
     2041 aagacgccac ttcgcacagc agctgcccgg tcaccggcgt cagattctgg taatactcac
     2101 catcggcagg ggctacccat tcaccgccaa taaagttgtc gtagcgggct tttaacttga
     2161 tggggaaacc atactcgccg ggcttaatct gtgctgaagg gggattattg gtcatgatcg
     2221 tctccttgcg gtgtgaggta taacaagggt agacgtgact ggcgaaatct tcgccagtcg
     2281 gtaacagctt tacgacagct atcacgaatt tacgggcaag gattacagag atagttgaaa
     2341 aagcggctga caatttgcca gccgttgttg gaatgatgat tacattgcgg cgcgataaat
     2401 caccacaatt tcttcgtgag ttgcctggat cgggttagta aagccacagg catctttcag
     2461 ggcattagtc gccagaaccg cgaaatcttc ttctttcacg ttcaggtcgc gtaggcctgc
     2521 cgggatatcc actttcttcg ccagttcacg gatggcgtta atgcaggctt cagcaccttc
     2581 cgcgtcgttt ttacctgtca cgttcacgcc cattgcagcg gcacagtcac gcagacgtgc
     2641 agcggcgact ttgctgttga atacctgaac atgcggcagc agaacggcgt tacatacacc
     2701 gtgtggcagg ttgtagaaac cgcccagctg gtgcgccatc gcatgaacat aacccagaga
     2761 agcattattg aacgccatac cggcaaggaa ctgggcataa gccattgctt cacgcgcttt
     2821 cgcattactg ccatcttcaa cggctaacgg caggttttcg gcaatcatgg tcacggcttt
     2881 cagtgcacaa gcgtcagtga tcggcgtggc ggcaatagaa acatacgctt cgatagcgtg
     2941 cgttaaggca tccatacccg ttgcggcggt cagtgacttc ggcataccaa tcatcagaga
     3001 ggagtcattg acagaaagca gcggagtcac gtgcttatca acaatcgcca ttttgttatg
     3061 acgcgcttcg tcagtgatga tgcagaaacg ggtcatttca gaggccgtac ccgccgtggt
     3121 attgatggcg atcatcggca gctgcggttt tgcagagcgg tcaacgcctt cgtaatcgcg
     3181 aatatcgccg ccattggctg ccaccagcgc aatacctttt gcgcagtcgt gtggagaacc
     3241 accgcctaag gagatcacgc tatcgcaatt attttcttta agtaatttca aacctgcggc
     3301 gacgttttcc gtggtggggt taggttgggt gccatcataa ataacgctaa aaatattgcg
     3361 ttcttccagt gctttttgca catcacccgc catacctaat ttcgttaaca tattgtcagt
     3421 gacaattaag gtacgggtaa atccataatc tgccatcata ttcattgcat cagtcaatga
     3481 atcagcgccg atgacattca cagaaggaat aaagaaagtt gaagatgcca taacacactc
     3541 ctgaaagtgt aaaaaagggt gggcagctta ctgaggattt tcattcgaaa acatgatcat
     3601 tattattaat tatcaaaatt aatctaataa aaagcacatt gtattcatta aacaatgtgc
     3661 tttatttaaa atcataagaa atttgtatta atttatttta aaaattaatt aatcatttcc
     3721 ttatttttcc ggaaataata atgcgtcgcg caataaatga tcatttccac gacgacgcgt
     3781 aaagccaatg cggtcaaaat attccagaat ctgaattgcc agctttcggc ctacgcctaa
     3841 gcgatcgcgg aaatccgccg cgcaggttga accacactcc tgatcgagat cgcggatcat
     3901 attggcaaat tcgacaatcc gatcgttacg gtaataacga tctttgacga tcgcggtaat
     3961 tattccttgc tgcgccgcct ggcgtagcgt caggcgcatt gcctgctcgt cggtacccgt
     4021 ctcttttgcc agatcacgca cccaccacgg ctcgtcacca aacagcggct ctgctttttg
     4081 ccagatggcc tgctgctctt cgctgaagcc cgctttgtga tccggcagat gcagccaacc
     4141 gtgatgactg aggatgtcgc cgctttcgcg catcttttca atcagcaaca gtactagcgc
     4201 ttcatcttcc attggcaacg ccatacgtcg cagacgttcg cgcccaggcc caggttcatc
     4261 gcgatgttgc tcatgataag tcgctaatgt gtcgagaatt ttccgttgcc agcgagcggc
     4321 aaccggcgca ttcaacaagc tataaccagc ctgaatatag ccaggctgtt gcagcagttc
     4381 gcgcatccct tcgccgttga gctggcgcgc ccaagcgaaa tcaggaagat taaccgcgcc
     4441 gcgttgcagg tgaacggcta acgcttcgct atcattctgt gccctggcga gggaagccag
     4501 ccattgcaga tactctggtt tacgtttacc gcgacgcggc gggttaagca tcacgacgcg
     4561 cgctccggcc agcgtgttgc gggcagagat atcgcgcaat accaggcggt cgttatctgc
     4621 cagccataac ggggtgtcga agaccagttc agcaaggtta tcttccagca gtgaaacgcg
     4681 tcccgtgacg tggctggcgg cgtggtgaat atgcagcggc tgccactggg tcagcggtgc
     4741 atgggtttga agctcgacaa tcacccgtgt gaacggctct ggcggcacat cggcaagcag
     4801 ccagtcgcca cggttaatct gctctttttc cgcatcaccc gcgatgtcaa gcgcgatacg
     4861 ctgcccggca ttggcggttt ctgttggctg gttttgcgca tgcagcgcac gtacacgcat
     4921 cggtttattt acaccagtca gccagagtga atcgcctacc ttcacttccc cgcttaacgc
     4981 cgtaccggtg acgaccagcc cggcaccttt tacggtaaat gcgcggtcaa tcgcgaggcg
     5041 gaaactatgt tggctggcgt gctcgcgttc cggcaactga agcagatgct cgcgcagggc
     5101 atcaattccc cgaccttcgg ttgctgcggt gataaacagt tttgcctcag caaaaccgta
     5161 ttcccgcaga acctccttta cctggcgttc aacctcatca acacgcgctt cgtccacgcg
     5221 atcggctttg gtcagcgcca ctgtcagcat cgggttaccg gtcagctgca aaatcgccag
     5281 atgctcacgg gtctgtgcca tcacgccgtc atcgcacgcc accaccaaca gcgcgtgatc
     5341 gataccacca acgcccgcca gcatgttgga aagaaacttt tcatgaccgg gaacgtcgat
     5401 aaaaccaggc acgcgaccat ccggctgcgg ccagtaggca tagccgagat cgatggtcat
     5461 gccgcgcttt ttttcttccg gcagacggtc agcatttacg ccagtaatcg cctgcaataa
     5521 ggttgttttg ccgtggtcaa cgtgtccggc agtcgcaata atcatttcaa caacatctcc
     5581 aaaaaccgtt gctcatcttc aaggcagcgt aaatccagcc acaatcgtcc gtcataaata
     5641 cgaccaatca ccggcactgg caattcacgc cagcgggcgg ctaatgactc aaggtggcta
     5701 ccgcgtccat catggggtgt aaacgttaat gccgcgctcg gcaggcgatc aaccggcagc
     5761 gaaccactgc caatctgcga aagacatggc ataacctgta ccgcaaactc cgcgccgtaa
     5821 tgtgcgacaa ggggagcctg taaacgttgt gcctggattt gaatgacctc tgcgctgcgg
     5881 gtaagcaggc gcagggtcgg taatttttca ctcagagctt cagggtgtaa ataaagacgc
     5941 aacgtggctt ccagcgccgc gagggtcatt ttatccgcgc gtaatgcacg cttcagcggg
     6001 tggctttgca ggcgggcgat catctctttt ttaccaacga taatcccggc ctgcggcccg
     6061 cctaacaact tgtcgccgga gaagctcacc agactgacgc ccgccgcaat caactcctgc
     6121 ggcattggct ctttcggcaa accgtactgg ctaagatcga ccagcgagcc actgcctaaa
     6181 tcagtcacca ctggaacatc cagctctttg ccgagcgcca ccagttcagc ttcgtctatc
     6241 gctttggtga acccctgaat gctgtagtta ctggtatgta ctttcatcaa cagtgcggta
     6301 ttttcattca ccgcctgacg ataatcattc gcgtgcgtgc ggttggtggt ccctacttcg
     6361 tgtagggtgc agcctgcctg acgcataaca tcgggaatac gaaacgcgcc gccaatctcc
     6421 accagttcgc cgcgagatac caccacctct ttcccgctgg cagtggccgc caacatcaat
     6481 aacaccgccg ccgcattgtt attgacgata caggcatctt ccgcccccgt aatacggcac
     6541 agcagctgcg ccagcgcccg atcgcgatgt ccgcgtccgg cgtcgtccag atcgtactcg
     6601 agggtcactg gcgaacgcat agcctgcgca acggcttcca ccgcggcttc cgcctgtaaa
     6661 gctcgcccaa ggttggtatg cagcacggtt cccgtcaggt tgatcaccgg acgcagcgcg
     6721 ctctgcgcct ctttcgtcaa ccgggcatcg acttcttgcg cccagttttc acaccacgca
     6781 ggcagcgtct ggctgccacg aatcacttct cgcgcttcgt cgagcatctg acgcaacaat
     6841 tccaccacgc gggtgtgacc ataagtatca cgcaaagaaa ggaaggagct atcgcgcaat
     6901 aagcgatcaa tagccggaag ttgactatag agggaacgcg tttcggttgt cataggaaac
     6961 ctggctgatc aaggccctct cacacggaga agggcgttta acataaccac ggattgtaac
     7021 gtgagatggg tcaggaggac atatcgcgca tcaagccttt ggcggttcgg tgcgagcaaa
     7081 actttcgcgg ctaaacaggt tttcgaccag tttgactaag tgtgggcggt cgacacacca
     7141 acctggcgca acgcggcgga aattgagata tccgacagca caggcaatgg cgatagtcgc
     7201 cagattgacc gtatcggttt tgagtgtgcc atcgaccaga tatccttcca gcacatccag
     7261 actgcggttg attttctccc gctggcgtaa caattcatct tcagactgct gcgccgccgg
     7321 acgcgcctgt tcacgcaccg ataccagccc ggcatccata atgccatccg ccagcgcctc
     7381 aattttgcgc acccgcaacg actccagcgg atcgcgcggc aacatcgccg gagcgacatt
     7441 cattaactca atatattcag cgatgatcgg cgaatcaaac cagcattcgc cctcttcggt
     7501 caccagcacc ggcactttcc ctaacgggtt aaattgcgcc acgccgttat ccgcgttata
     7561 gggcagttca ttaataaatt cgaaagttat gcccttttct aacaacagaa taaaaagttt
     7621 gcgtacaaac gggctggtgt agctaccgac gagtttcatg ccgagtcctt tgtgcgagga
     7681 aaaatatcag tatggctcag gctcacgcaa aacacaggtg atataagtct caatgatcca
     7741 aataaagata atgcatccaa ctggtcattc ttagcagcac tttccgcatc accgaaacat
     7801 ggctgaaatg gtcggagtaa accgccacct gggcgtagat gccgtcgggt aaggcatcga
     7861 tatcatcgtt agggtccagt tcaatggttc ccagcacacc gtccgtgccg ggaacgaccg
     7921 ttaatgattg caataccccc tgcgcctgat aagaaccgcc tggcacgaca ggtaaaatac
     7981 tagtcaattt gccgtggaac acctgcccag gtagcgcgtt aaacaccact tccgcatcat
     8041 cgccaggttt cagacgtaac agcgagtttt gccgaaattg ggcgacaatt tgccgttttt
     8101 gctcggggat gaagaccatc accggacgca gcggcaaggc agctgcgtat gtacctgggc
     8161 ggatcagtac ctgagtgacg tagccattgc ttggtgcgcg aatgacagtc tgctcaagat
     8221 tatattttgc ttcagtaagt tgcgctctta agctcacaat ctgcgattgc tcgccgttga
     8281 ccatactgtc gagctgactc tggatctgcg cctgctccgc caccgagcct ttcactaacg
     8341 catcctgcgc gaggaaattt tgccgcgcat cgtcgatgtc acgttccgag aacggattca
     8401 ccgccgcctg gctgcctttc aggtaacgtt gataattttt aaacagacgg tcgcgctccg
     8461 ctgaaacctg ggtggtgttg gcctgcgctt cagtgagctg cgcacgcagc gtctttatat
     8521 tatgcgtcgc cgtcatcagg tcagcctgaa gtctgtcaac tcgcgcctgg taacgaacag
     8581 ggtcgagctt aaaaagcacc tcgccctttt gaataagctg attattcttg tcagtgactt
     8641 cagtaacaat tcccgtcacc tgtggcgtga tagggatcgc tatcactgcc ttttgcgcgg
     8701 taaaagtgta agggtggttg tagttcatca acaaaatcaa accactcacc agaaacacgc
     8761 ctcccagcgc cgccgtcgcc agcgccacgt aagttaaaac aatcaataga tccataatta
     8821 gatctccggg aacgtggaat agtcagtctt tttctcggcg gcgagttgat gctccagccg
     8881 ggaaattcgg tcagaaagtg cggcgatttc cggttcagtc gctttctcct gcgacgcaac
     8941 atgtgactgc ataccccagc cacgctccgg ttgatagagc gtcgcccaga tccacagaaa
     9001 cggccagata acatgcagag taaacaggct cacccagccc gccgtatgaa tagcgtcggc
     9061 atgggggtga ttgcgctttt tggcaatcag ataagggatg tcatgtatcg cgatgattcc
     9121 ataaaaaatc accagaaaga cgaagatcag cactcccaac gcgaaatagt ttagaaacat
     9181 atctgcctcg gattcacgtt tatcagtgtt gttttttggc tggcagccag aagggagtca
     9241 ggctgatatt ttgacaataa tccgggttcg cgattctcgc cataacacca aagaataatt
     9301 tttagaggtg atgagttgct tagttacata acgattgtat gacgaaggca taacatgctg
     9361 tagatcacat caggtgaacg ccgtaagaaa atatcttgtg attcagatca caaagattca
     9421 acaaaccatc aaaacaaaaa tgtgacatta ctcacattta aatgccattt ttagcgaaaa
     9481 tcaccgcctt gtttcttttt tatacaggcg ttttgtgatg agcgtcacgt caattacctc
     9541 tctaccccct atatttatgt gactgatatc acacaaaagc ccgtcgactg gacagttaac
     9601 cgattcagtg ccagatttcg cagtatctac aaggtccggc tacctctgcc gccacattaa
     9661 caaaaaacct cgggcttcca gcctgcgcga cagcaaacat aagaaggggt gtttttatgt
     9721 catccgatat taagatcaaa gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc
     9781 caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc gttatttatt ccaacagggt
     9841 ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat gatcacttat ctcctaccgc
     9901 tgctgatcgg ttataccggt ggtaagctgg taggcggcaa acgtggtggc gtagtcggtg
     9961 ccattaccac catgggcgtt atcgtcggcg cagacatgcc gatgttcctc ggttctatga
    10021 ttgcaggtcc gctgggcggc tggtgcatta agcacttcga ccgctgggta gacggtaaga
    10081 tcaaatccgg ttttgagatg ctggtgaata acttctccgc aggcatcatc gggatgatcc
    10141 tcgctattct ggcattcctc ggcattggcc cgattgttga agccctgtcc aaaatgctgg
    10201 ctgcgggcgt taacttcatg gttgtccatg acatgctgcc gctggcgtct atctttgttg
    10261 aaccggcgaa aatcctgttc ctcaacaacg ccattaacca cggtatcttc tcgccgctgg
    10321 gtattcagca gtcccatgaa ctgggtaaat caatcttctt cctgattgaa gctaacccag
    10381 gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt
    10441 ctgcgggcgg tgcggcaatc atccacttcc tgggtggtat ccacgaaatc tacttcccgt
    10501 atgtgctgat gaatccgcgt ctgatcctcg cagtcatcct cggcggtatg actggcgtgt
    10561 tcacgctgac tatcctgggc ggtggtctgg tttctccggc atctccgggt tctatccttg
    10621 ctgtactggc gatgacacca aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg
    10681 cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag
    10741 aagatgatat tgaagcagca actcgtcgta tgcaggacat gaaagctgag tctaaaggca
    10801 catctccgct atctgcgggc gatgtgacta acgacctgag ccacgtacgt aaaatcatcg
    10861 ttgcctgtga cgccggtatg ggttcaagtg cgatgggcgc aggcgtgctg cgtaagaaaa
    10921 ttcaggatgc aggtctgtcg cagatttcgg tcactaacag cgcgatcaac aacctgccgc
    10981 cagatgtgga cctcgtcatc actcaccgtg acctgaccga acgcgctatg cgccaggttc
    11041 cgcaggcaca gcatatttcg ctgaccaact tcctcgacag cggcctgtac accagcctaa
    11101 ccgaacgtct cgttgctgcc cagcgccata ctgaaaacga agtgaaagtg aaagacagtc
    11161 tgaaagacag ctttgacgat tccagtgcta acctgttcaa actgggcgcg gagaacatct
    11221 tcctcggtcg caaagcagca accaaagaag aagcgattcg ttttgctggc gagcagctgg
    11281 tgaaaggcgg ttacgttgag ccggaatacg ttcaggcgat gctggatcgt gaaaaactga
    11341 ccccgactta tctgggtgag tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc
    11401 gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag
    11461 aagaagatga cattgcccgt ctggtgattg gtattgcagc ccgtaacaac gagcacattc
    11521 aggttatcac cagcctgacc aatgcactgg atgatgagtc cgtcatcgag cgtctggcac
    11581 acaccaccag cgtggatgaa gtgctggaac tgctggcagg tcgtaagtaa tccaatccta
    11641 ccctctcctc acggagaggg tttgggtgag ggaaaagcct caccccagcc ctctcgggta
    11701 aaaacattga tgaaggttaa tactatgaaa gcattacatt ttggcgcagg taatatcggt
    11761 cgtggcttta tcggtaaact gctggcagac gcgggtatcc aactgacgtt tgccgatgtc
    11821 aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct atcaggttca tgtggtcggt
    11881 gaaaccgagc aggtagatac cgtttccggc gtcaatgctg tcagcagcat tggtgatgat
    11941 gtcgttgatc tgattgctca ggtcgattta gtcactaccg ccgttggccc ggttgtgctg
    12001 gaacgtattg ctccggctat cgccaaaggg ctggtgaaac gcaaagaaca aggtaatgaa
    12061 tccccgctga acatcatcgc ctgtgaaaac atggtacgcg gcaccacgca gctgaaaggt
    12121 catgtgatga acgccctgcc ggaagacgcc aaagcgtggg tagaagagca cgttggcttt
    12181 gtcgattccg ccgttgaccg catcgtaccg ccttcggctt cggcaactaa cgatccgctg
    12241 gaagtgacgg tagaaacttt cagcgaatgg attgtcgata aaacgcagtt caaaggcgca
    12301 ctgccgaaca tcccaggcat ggagttaacc gacaacctga tggcatttgt cgaacgtaaa
    12361 ctcttcaccc tgaacacggg tcatgctata accgcgtacc tcggaaaact ggccggtcat
    12421 cagaccattc gtgacgctat tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg
    12481 gaagaaagtg gtgcggtact gatcaagcgc tacggctttg acgcagacaa acatgcggcg
    12541 tacatccaga aaatcctcgg tcgttttgag aacccgtatc tgaaagatga tgtagagcgc
    12601 gtaggccgtc agccgctgcg taaactgagt gctggcgacc gtctgatcaa gccactgctc
    12661 ggtacgctgg aatacagcct tccgcacaag aatctgattc aggggattgc tggtgcaatg
    12721 cacttccgca gtgaagatga tccgcaggct caggaactgg cagcactgat cgctgacaaa
    12781 ggtccgcagg cggcgctggc acagatttcc ggtcttgatg ccaacagcga ggttgtatcc
    12841 gaggcggtaa ccgcttataa agcaatgcaa taatggtgga ccaggcgcag gacaccctgc
    12901 gcccgaataa cagattgtca gatatgcagg caacaatgga acaaacccag gccgttgaaa
    12961 accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg aagctttctg atcaccgccg
    13021 tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc aaagacgatt
    13081 acgcggtgaa gtatgctgta gaaccgttac tcgacggcga cggtccgctg ggcgatcttt
    13141 ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg
    13201 cggaactgct gatggcattg cgtgaagagc taaatcacga cggcaacgag tacgccttta
    13261 ccgacgacga aatccttgga ccctttggtg aactgcactg cgtggcggcg ttaccaccgc
    13321 cgccacagtt tgaaccagca gactccagtt tgtatgcaat gcaaattcag cgctatcaac
    13381 aggctgtgcg atcaacaatg gtcctttcac tgactgagct gatttccaaa atcagcttaa
    13441 aaaaagcctt tcaaaagtaa gcaacgtctg cttactgccc ctctacctgc ttcggccgat
    13501 aaagccgacg ataatactcc agacgttgta gatataacgg cacgctttca tcgggtattc
    13561 cggacggaat cgcgttacgg ggaggaagtt ttttcagata ctcccggaac gcctggcttg
    13621 atgccatgaa atctacggct ttatcgataa gaagcggaac gttttcacct aatttgccca
    13681 tgatgatatc tccgtattac cccgcgccgg gaatgcgcgg ccgccaattt tagtttaggt
    13741 tcccggtaaa gatgacatta aggaaaacgt gctgaatcct caataatcat ccgctgctgt
    13801 ttattatctt tttctataga tttcttaatt aatcaacgaa ttgcgtagtt tttgagcatg
    13861 ttaatgatta cgttgcagaa ctattacaat gtcatcagtt gtgaacaaag cacctggtcg
    13921 cgcatactag gggctataaa tttatcttta tcagaagcca tcacatgaaa gaagtcgaaa
    13981 aaaacgaaat caagcgtctc agcgatcgcc tggacgccat ccgccaccag caggccgatc
    14041 tgtcgctggt tgaagccgca gacaaatatg ccgagctgga aaaagagaaa gccacgctgg
    14101 aagcagaaat tgctcgcctg cgtgaggttc atagccaaaa actgagtaaa gaagcacaga
    14161 agctgatgaa gatgccgttc cagcgcgcaa ttaccaaaaa agagcaggct gatatgggca
    14221 agctgaagaa aagtgttcgc ggactggtgg ttgtgcaccc aatgaccgcg ctggggcgcg
    14281 aaatgggcct ggaagagatg actggatttt ctaagactac gttttaagcc cctcagacga
    14341 ctggttcgat cgtatcaacc cgcatctggc ttgccttagc tggtcaatgc ggggactctc
    14401 ctgtctactc gcctaattac c
//
LOCUS       DAZSNM010000099        14281 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZSNM010000099 DAZSNM010000000
VERSION     DAZSNM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14281
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.099"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            172..1191
                     /locus_tag="ACHW42_002444"
     CDS             172..1191
                     /locus_tag="ACHW42_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8944234.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
     gene            1301..2173
                     /locus_tag="ACHW42_002445"
     CDS             1301..2173
                     /locus_tag="ACHW42_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFR8944235.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            2507..5629
                     /gene="agn43"
                     /locus_tag="ACHW42_002446"
                     /gene_synonym="flu"
     CDS             2507..5629
                     /gene="agn43"
                     /locus_tag="ACHW42_002446"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFR8944236.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            5750..8266
                     /locus_tag="ACHW42_002447"
     CDS             5750..8266
                     /locus_tag="ACHW42_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944237.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            8342..8797
                     /locus_tag="ACHW42_002448"
     CDS             8342..8797
                     /locus_tag="ACHW42_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFR8944238.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            8940..9029
                     /locus_tag="ACHW42_002449"
     CDS             8940..9029
                     /locus_tag="ACHW42_002449"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFR8944239.1"
                     /translation="MTAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            9208..10026
                     /locus_tag="ACHW42_002450"
     CDS             9208..10026
                     /locus_tag="ACHW42_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR8944240.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            10368..10841
                     /locus_tag="ACHW42_002451"
     CDS             10368..10841
                     /locus_tag="ACHW42_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR8944241.1"
                     /translation="MKTLSQNTTSSACAPETDLQQLVATLVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            10857..11333
                     /gene="radC"
                     /locus_tag="ACHW42_002452"
     CDS             10857..11333
                     /gene="radC"
                     /locus_tag="ACHW42_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR8944242.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            11420..11641
                     /locus_tag="ACHW42_002453"
     CDS             11420..11641
                     /locus_tag="ACHW42_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFR8944243.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            11721..12098
                     /gene="cbeA"
                     /locus_tag="ACHW42_002454"
     CDS             11721..12098
                     /gene="cbeA"
                     /locus_tag="ACHW42_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFR8944244.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            12145..12522
                     /locus_tag="ACHW42_002455"
     CDS             12145..12522
                     /locus_tag="ACHW42_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFR8944245.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            12519..13007
                     /locus_tag="ACHW42_002456"
     CDS             12519..13007
                     /locus_tag="ACHW42_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFR8944246.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            13027..13224
                     /locus_tag="ACHW42_002457"
     CDS             13027..13224
                     /locus_tag="ACHW42_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFR8944247.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            13309..14151
                     /locus_tag="ACHW42_002458"
     CDS             13309..14151
                     /locus_tag="ACHW42_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFR8944248.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
BASE COUNT         3598 a         3723 c         3757 g         3203 t
ORIGIN      
        1 gatttttcac catgaaaaaa acatcaatgt atctctgtat aaaaataatg ataacatgac
       61 ataaacattt aaaaacataa ataaacagca acaaaaacat ttgataatca cttagtgatt
      121 aaatacaatt aagacgattt tgttataaga gaggcaccat ggagtgagat aatgaataac
      181 gaacagatgc ttatcaaatg cagagatccc cgtaaatgtc ggttttatgc cgaatatctg
      241 aatcacaaat accctaatga attccctcac tttgcgcctg acaacccaca aaattgtatc
      301 aggaatgtta atttattttt aaaggaaaag tattgtaatg tttcacctcc cccctttggt
      361 acttttggta ctactgaaga agaagcactc aataacgaaa ttaatgagct tgaaattatt
      421 gccgggcagg caatactgcc tgaagagagt tttctctggt tgaaaactga cgaacttgcg
      481 actttcttta cctggactac tatttatctg tcgaaggaag accatacaaa attcccgaaa
      541 ataaaccata attcatcagg aataaaaaac ggaattgaag tcaggaaaat acgaacagtt
      601 accgatgaaa cgttaaacac ttacaaaaaa agtaaccttc cggataatcc atcatcccat
      661 aacgaacgaa taaaagtaat catttcatat tttgactcct gccattcttt aattaataaa
      721 aatgcaaaaa gcatatacct cgaggaactc aggcaggcgt ggcttaaaaa gaaaagcgct
      781 tgtcaaaagt ttaagtggct ccttcctgac gatgatgaaa tgtgtcagtg gttctggaca
      841 agactgaaac aaaaacagga tgaaaaacat ccagtacata acccagtgac ccggtggttc
      901 ataccatctt caacgaaaga atattatctt gcctctcttg tcgcctttgc tttatggaaa
      961 gaagcgcctg ataccattga actgttcaga acacgaataa atcatgcctg gcatcagaaa
     1021 aaacaaaagg acaaatatcg gagtgatggt aaaaaggcca ttaatataca tataagaagc
     1081 aatgtgaaag aaatgcttga tgagctcaga actgtttacg gtctgagaac aggagagttt
     1141 tttgaaatgc tgattacaga gaaatatctt gaacacaagc gaaggaagta attcattacc
     1201 tgttacattc taaataatct cagacagata ttatctccgt gaatcccccg ccacctttcc
     1261 ggtgcgcggg gttttgtctt ttttcaccgg gaatacatgt atgaatccgt ctgatgccat
     1321 tgaggcaatt gaaaaaccgc tctcctccct gccttactca ctttcccgtc acatcctgga
     1381 acatctgcgc aaactcacca gtcacgaacc cgtgattggc attatgggta aaagcggggc
     1441 cggtaaatct tcactctgta atgcactgtt tcagggggag gtcacaccgg tcagtgatgt
     1501 tcacgccggc acccgggaag tgcagcgctt ccgtctgagt ggtcacggtc acagcatggt
     1561 tatcactgac ctgcccgggg tgggcgagag ccgggacagg gatgcagagt atgaagccct
     1621 gtaccgtgac attctgcctg aactggacct ggtgctgtgg ctgattaaag ccgatgaccg
     1681 ggccctgtct gtggatgagt atttctggcg acacatcctg cagtgcggac atcagcaggt
     1741 gctgtttgtg gtgacgcagg ctgacaaaac ggaaccctgc catgaatggg atatggcagg
     1801 tattcagcct tctcccgcac aggaacagaa cattcgtgag aaaacggagg cggtattccg
     1861 tctgttccgg cccgtacatc cggttgttgc cgtatcggcc cgcaccggct gggaactgga
     1921 tacgctggtc agtgcgctca tgacagcgct tcccgaccat gccgccagcc ccctgatgac
     1981 ccgactgcag gacgagctgc gcacggagtc tgtccgctct caggcccgtg aacagtttac
     2041 cggtgcggtg gaccggatat ttgacacagc ggagagcgtc tgtgttgcct ctgttgtccg
     2101 tacggccctg cgcgctgttc gtgacaccgt ggtctctgtt gcccgcgcgg tatggaactg
     2161 gatcttcttc tgaacctgtt gtggatgatg tcctccctgc ctctgagtct gctcacaaaa
     2221 gcgctgtttt cgttactgtc tctcttgtcc gtgcaataga ctgataatag aataaaacga
     2281 tcgataccta ttttaccgat cgttaatatc gatcgttatg ctaataataa ctcctgttag
     2341 caacgtgcgc agatacacac agacatgaga ttcagggaac aacagagcca cacgtcagaa
     2401 acttccgtca gctggacctc cggcactgta accctttacc tgccggtatc cacatctgtg
     2461 gataccggct tttttattca ccctcactct gattaaggaa atgctgatga aacgacatct
     2521 gaatacctgc tacaggctgg tatggaatca cattacgggc gctttcgtgg ttgcctccga
     2581 actggcccgc gcacagggta aacgtggcgg tgtggcggtt gcactgtctc ttgccgcggt
     2641 cacgtcactc ccggtgctgg ctgctgacat cgttgtgcac ccgggtgaaa cagtgaatgg
     2701 cggaacactg gtaaaccatg acaaccagtt tgtatccgga acagctgatg gcgtgactgt
     2761 cagtaccggg cttgagctgg ggccggacag tgacgaaaac accggcgggc aatggataaa
     2821 agcgggtggc acaggcagaa acaccactgt caccgcaaat ggtcgtcaga ttgtgcaggc
     2881 aggaggaact gccagtgata cggttattcg tgatggcgga gggcagagcc ttaacggact
     2941 ggcggtgaac accacgctgg ataacagagg tgagcagtgg gtacacgggg gagggaaagc
     3001 tgccggtaca attattaacc aggatggtta ccagaccata aaacatggcg gactggcaac
     3061 cggaaccatc gtcaacaccg gtgcagaagg tggtccggag tctgaaaatg tgtccagcgg
     3121 tcagatggtc ggagggacgg ctgaatccac caccatcaat aaaaatggcc ggcaggttat
     3181 ctggtcttcg gggatggcac gggacaccct catttacgcc ggtggtgacc agacggtaca
     3241 cggagaggca cataacaccc gactggaggg gggtaaccag tatgtacaca acggtggcac
     3301 ggcaacagag acgctgataa accgtgatgg ctggcaggtg attaaggaag gaggaactgc
     3361 cgcgcatacc accatcaacc agaaaggaaa gctgcaggtg aatgccggcg gtaaagcgtc
     3421 tgatgtcacc cagaacacgg gcggtgcact ggttaccagt acagctgcaa ccgtcaccgg
     3481 cacaaaccgc ctgggagcat tctctgttgt ggagggtaaa gctgataatg tcgtactgga
     3541 aaatggcgga cgcctggatg tgctgaccgg acacacagcc actaataccc gcgtggatga
     3601 tggcggaacg ctggatgtcc gcaacggtgg caccgccacc accgtatcca tgggaaatgg
     3661 cggtgtactg ctggccgatt ccggtgccgc tgtcagtggt acccggagcg acggaaaggc
     3721 attcagtatc ggaggcggtc aggcggatgc cctgatgctg gaaaaaggca gttcattcac
     3781 gctgaacgcc ggtgatacgg ccacggatac cacggtaaat ggcggactgt tcaccgccag
     3841 gggcggcaca ctggcgggca ccaccacgct gaataacggc gccatactta ccctttccgg
     3901 gaagacggtg aacaacgata ccctgaccat ccgtgaaggc gatgcactcc tgcagggagg
     3961 cgctctcacc ggtaacggca gcgtggaaaa atcaggaagt ggcacactca ctgtcagcaa
     4021 caccacactc acccagaaag ccgtcaacct gaatgaaggc acgctgacgc tgaacgacag
     4081 taccgtcacc acggatgtca ttgctcagcg cggtacagcc ctgaagctga ccggcagcac
     4141 tgtgctgaac ggtgccattg accccacgaa tgtcactctc gcctctggtg ccacctggaa
     4201 tattcccgat aacgccacgg tgcagtcggt ggtggatgac ctcagccatg ccggacagat
     4261 tcatttcacc tccacccgca cagggaagtt cgtaccggca accctgaaag tgaaaaacct
     4321 gaacggacag aatggcacca tcagcctgcg tgtacgcccg gatatggcac agaacaatgc
     4381 tgacagactg gtcattgacg gtggcagggc aaccggaaaa accatcctga acctggtgaa
     4441 cgccggcaac agtgcgtcgg ggctggcgac cagcggtaag ggtattcagg tggtggaagc
     4501 cattaacggt gccaccacgg aggaaggggc ctttatccag gggaataagc tgcaggccgg
     4561 tgcctttaac tactccctca accgggacag tgatgagagc tggtatctgc gcagtgaaaa
     4621 tgcttatcgt gcagaagtcc ccctgtatgc ctccatgctg acacaggcaa tggactatga
     4681 ccggattctg gcaggctccc gcagccatca gaccggtgta agcggtgaaa ataacagcgt
     4741 ccgtctcagc attcagggcg gtcatctcgg tcacgataac aacggcggta ttgcccgtgg
     4801 agccacgccg gaaagcagcg gcagctatgg cttcgtccgt ctggagggtg acctgctcag
     4861 aacagaggtt gccggtatgt ctgtgaccgc gggggtatat ggtgctgctg gccattcttc
     4921 cgttgatgtt aaggatgatg acggctcccg tgccggcacg gtccgggatg atgccggcag
     4981 cctgggcgga tacctgaatc tgatacacaa cgcctccggc ctgtgggctg acattgtggc
     5041 ccagggaacc cgccacagca tgaaagcctc atcggacaat aacgacttcc gcgtccgggg
     5101 ctggggctgg ctgggttcgc tggaaaccgg tctgcccttc agtatcactg acaatctgat
     5161 gctggagccg caactgcagt acacctggca gggactctcc ctggatgacg gccaggataa
     5221 cgccagttat gtgaagttcg ggcatggcag tgcacaacat gtgcgtgccg gcttccgtct
     5281 gggcagccac cacgatatga actttggtaa aggcacctca tcccgtgaca ccctgcgcgg
     5341 cagtgccaaa cacagtgtgc gtgaactgcc ggtgaactgg tgggtacagc cttctgttat
     5401 ccgcaccttc agctcccggg gggacatgag catgggtaca gccgcagccg gcagtaacat
     5461 gacgttctca ccgtcacaga atggtacgtc actggacctg caggccggac tggaagcccg
     5521 tgtccgggaa aatatcaccc tgggcgttca ggccagttat gcccacagca tcaacggcag
     5581 cagcgctgaa ggttataaca gtcaggccac actgaatgta accttctgat aattctgcat
     5641 tgcctctctg tggtcccggt catgatgacc gggacccgga cgggggaaaa cgtttcagtg
     5701 ccacattcac tggcattcac aataacatga tattcatcac ggagtgacta tgttacagat
     5761 agtcggcgcg ctgattctgc tgatcgcagg atttgccatt cttcgccttt tgttcagagc
     5821 attaaccagc acagcgtctg cgctggcagg gttcatattg ctgtgtctgt tcggtccggc
     5881 tttactggct ggctatatca ctgaacgcat aacccggtta ttccatattc gctggctggc
     5941 aggcgtattt ctgacgattg ccggaatgat catcagcttc atgtggggac ttgatggtaa
     6001 acatatcgca ctggaggctc atacttttga ctctgtaaaa tttattctga ccaccgctct
     6061 cgccgctggt ctgctggctc ttcccgtgca gataagaacc attcagcaga acgggctcac
     6121 acctgaagat atcagcaagg aaattaacgg gtattactgc tgtttttata ctgctttttt
     6181 ccttatggcg tgttctgcat acgcaccatt gatcgcattg cagttcgata tttcaccctc
     6241 actgatgtgg tggggcgggt tgttgtactg gctggctgca ttagtgacgc tgctatgggc
     6301 ggccagccag atccaggcgc tgaaaagact gaccagtgcc atcagccaga cactggaaga
     6361 acaaccggtg ctcaacagta aatcgtggct gagcagtttg caaaacgatt acagccttcc
     6421 tgaaacgctg acggagcgca tctggctgac actcatttca caacggattt cccggggaga
     6481 actgagggaa tttgaactgg cagacggaaa ctggttactg aacaatgcct ggtatgaaag
     6541 aaacatggca gggtttaacg aacagttgaa agagaacctg tcattcacac ctgatgaact
     6601 gaaaacgctc ttccggaacc gcctgaattt atcaccggaa gcgaatgacg attttctcga
     6661 tcgttgcctg gacggcggtg actggtaccc cttttcagaa ggccgccgtt ttgtatcatt
     6721 ccaccacgtg gatgagcttc gtatctgtgc ctcctgcggg ctgacagaag tacatcatgc
     6781 cccggaaaat cataagccgg ctccggaatg gtactgctcc tctctttgtc acgaaacaga
     6841 aacactgtgt caggacattt atgaacgttc ttacaccggt tttatttccg atgcaacggc
     6901 gaatggtctg attctcatga aactgccgga aacctggagt acaaatgaga aaatgtttgc
     6961 ttccggaggg cagggacatg ggtttgccgc tgaacgggga aaccatattg tcgacagagt
     7021 ccgtctgaaa aacgcacgga tcctcggtga taataatgcc agaaatggag cagacagact
     7081 ggtcagcgga acagaaatcc agacgaaata ttgttcaact gcagcccgta gcgtcggtgc
     7141 ggcattcgac ggacaaaacg gacagtatcg ttacatggga aatcatggtc ccatgcaact
     7201 ggaagtcccc cgtgatcagt atgccggcgc tgtggaaacc atgaagaata agatccgcga
     7261 aggtaaagta cccggtgtaa ccgatcccgc agaagcgtcc cggctgattc gtcggggaca
     7321 tctgacttat acccaggccc gtaatatcac ccggttcggg accatcgaat cggtcactta
     7381 tgatattgcc gaggggtcgg ttgtcagtct ggcggccgga gggatcagtt ttgccctgac
     7441 ggcatcggtc ttctggctca gcaccggcga tcgcgatgct gccctgcaga cagctgctgt
     7501 ccaggcagga aaaaccttca cccgcacact ggctgtctac gtcacaaccc agcaacttca
     7561 ccggctcact gttgttcagg gtatgctgaa gcatattgat ttttcgacgg ccagcccgac
     7621 tgtccggcag gcgcttcaga aggggaccgg tgcaggaaat atcagtgccc tgaacaaagt
     7681 gatgaagggt acgctggtga catctctggc actggtagct gtcacaacca gccctgacat
     7741 gatcaaaatg ttgcggggac ggatctccgg tgcgcagttc atcaggaatc ttgccgtggc
     7801 atcttccggt gtggcaggtg gtgctgtcgg gtcagtggcg ggcgggatat tgttcagtcc
     7861 actgggacca tttggtgcac tgacagggcg tgtggttggc ggtgttctgg ggggaatgat
     7921 tgcctccgct gtatcaggaa aaattgccgg agcgctggtt gaagaagatc gcgtcaaaat
     7981 tctggcaatg attcaggagc aggtgacatg gcttgccggc agtttcctgc tgaccggaca
     8041 tgagattgaa aatctgaacg cgaatctggc ccgtgttatc gatcagaatg ctctggagat
     8101 cattttcgcc gccggtatac aacaacgggc cgcgaccaat atgttaatca aaccactggt
     8161 ggtcagtatc atcaggcaac gccccgtcat ggaatatgat gcatcccatc tcggcaagat
     8221 ggttaaccgg ctggaagaag cattcccccc ggagctcccg gcataacgtg tgttctgctc
     8281 cttttaggtt ttatcacctg atttgccagt gtcatcgtca tgactcagag aggtaaatac
     8341 catgattcac ctgttcaaaa cctgcatgat taccgccttc attctggggt taacgtggtc
     8401 tgccccactc cgggcacagg atcaacgtta catcagtata cgcaatacag atacgatatg
     8461 gctcccggga aatatttgtg cttaccagtt ccggctggat aatggcggaa acggtgaagg
     8521 atttggcccc ctcaccatca ctctgcaact caaagacaaa tatggtcaga cgctggtgac
     8581 cagaaaaatg gaaacggaag cttttggtga cagtaatgcc acgcgaacca cagacgcatt
     8641 tctggaaacg gagtgcgtgg aaaatgtcgc cacaaccgaa atcattaaag caactgaaga
     8701 aagtaacggc catcgtgtca gtctgccgtt atcggttttc gatccccagg actaccatcc
     8761 actgctgatt accgtttccg gaaaaaatgt taactgatcc ctgaagtcag tttcatgctt
     8821 cagcctctgc cacaacgtat ggcagaggct atttatcagg agaaaccaga tatgccgggt
     8881 tacacacaga gtatgtcctg gctgaaggat ctttctcata cgggcaggcg gttgctgtca
     8941 taaccgctta ccgcaatgtg tttatccagg atgacccggg aatgcatttc cgtcgggtta
     9001 tccgcaacgc ggaaggtcag cgacgctgac gcagccggaa ctctgaagct gatgccggaa
     9061 aacagcttaa tgcgtggctc gccagtggag ggcttctcag acaataaact tcattgattt
     9121 catccatcag gccgcgtctt ctccgggaga cgcggctttt ttcatttgtt gcaccagtaa
     9181 atcttaacca ccgataagga gcaaagtatg caattagtca gtcgttttgg ttatgcgaac
     9241 cagatacgcc gtgaccgtcc gctgacacat gaagagctga tgcaccatgt gccggggatt
     9301 ttcggggaag agaaacacac gtcccgaagc cagaactata cgtacatccc caccatcacc
     9361 gtactggaaa gtctgcagcg ggaaggcttc cagccctttt tcgcctgcca gacccgtgtg
     9421 cgcgacccgg gccgccgggg atacacaaaa cacatgctgc gtctgcggcg ggccggagag
     9481 ataaacggag aacatgtccc tgaaattatt ctgctcaact cccatgacgg tacctccagc
     9541 taccagatgc tgccgggtta cttcagattc gtctgccaga acgggtgtgt ctgtggtcag
     9601 tctctggggg aagtgcgtgt tccgcaccgg ggaaacgtgg tggagaaagt tattgaaggg
     9661 gcttacgaag tggtgggcgt gtttgaccgg attgaggaga aacgtgatgc catgcagtcg
     9721 ctggtcctgc cgccaccggc acgccaggcg ctggcacagg cggcactgac ttaccgttat
     9781 ggtgacgaac atcagcccgt caccaccgcc gacattctga cgccacgacg ccgggaggat
     9841 tacggtaagg acctgtggag tacttatcag accatccagg agaatatgct gaaaggcggg
     9901 atttccggcc gcagtgcaaa aggaaaacgt atccacaccc gtgccattca caacatcgac
     9961 accgatatta agctcaaccg cgcattgtgg gtgatggcag aaacgctgct ggagagcctg
    10021 cgctgatgca gcatgtccct gaaggcgtga ttcaggcacc agaatcaccc gacgccccgg
    10081 aggcgctgcc ggggtgtcgt tccatttctg atgatgacct gtgcgcctgg tgtacccgtc
    10141 tgctttaccg tcccggagaa atcagtctgt gcagactgag tatacaggca atggcatctg
    10201 gccatccgtc tgtgaccgga acggttacgc gtacggctgt ccggaatttc agccgaatat
    10261 cattcatccc tgatattacc gggtcatcac ggtcagtgat gaacgtgatg ttgccagctc
    10321 ccgaccttta ctccctctca tttattcaca ttacaaggat ttcatacatg aaaacattgt
    10381 ctcagaacac cacctcttcg gcctgtgcac cggagactga cctgcaacaa ctggttgcca
    10441 ccctcgtccc tgatgaacag cgcatcagct tctggccgca gcactttggc ctcattccac
    10501 agtgggtgac cctggagccc cgtgtcttcg gctggatgga ccgtctgtgc gaagactact
    10561 gcggtggtat ctggaatctg tacaccctga acaacggcgg ggcatttatg gcacccgaac
    10621 cggatgacga tgatgacgaa acatgggtac tgttcaatgc catgaacggt aaccgcgctg
    10681 aaatgagtcc ggaagctgcc ggcattgctg cctgtctgat gacgtacagt catcatgcct
    10741 gtcgtacgga gtgttatgcc atgacggtcc attattaccg gctgcgggat tacgccctgc
    10801 agcatccgga atgcagcgcc attatgcgca tcatcgactg aatggtggca gggacaatgc
    10861 agcagctttc ctttctgccc ggagaaatga caaccagaga gcgcagcctc attctgcggg
    10921 ccctgaaaac cctggaccgc catcttcatg aacccggcgt ggccttcacc tccacgcatg
    10981 cggcacggga atggctgatt ctgaacatgg cgggactgga gcgtgaagag ttccgggtgc
    11041 tgtatctgaa caaccagaat cagctgattg ccggtgaaac cctcttcacc ggcaccatca
    11101 accgtacgga agtccatccc cgggaagtga ttaaacgcgc cctgtaccac aatgccgctg
    11161 ccgtggtgct ggcgcacaat cacccgtccg gtgaagtccc ccccagcaag gcagaccgcc
    11221 ttatcactga gcgtctggta caggcactgg ccctggttga tatccgggta ccggaccatc
    11281 tgatagtcgg tggcagccag gttttctcct ttgcggaaca cggtctgctt taacccgtca
    11341 ccgtcacaat caccttcata tcacttcagt ttctctttct cagctgtttc ttactttcac
    11401 attcaggagg actattctca tgaaaatcat cacccgtggt gaagccatgc gtattcaccg
    11461 tcagcatcct gcatcccgtc tttttccgtt ctgtaccggt aaataccgct ggcacggtag
    11521 cacggataca tataccggcc gtgaagtgca ggatattccc ggtgtactgg ctgtgtttgc
    11581 agaacgccgt aaggacagtt ttggtccgta tgtccggctg atgagcgtca ccctgaactg
    11641 agtctggaag ggaattctga tgagcagaat tatcgccact accggaccat tcttaaccga
    11701 ttttctgtga ggactttatc gtgtcagaca cactccccgg gacaacgcct cccgacgaca
    11761 atcacgaccg cccctggtgg gggctgccct gcaccgtgac gccctgtttc ggggcccgac
    11821 tggtacagga gggtaaccgc ctgcattacc ttgcagaccg cgccggtatc agaggcaggt
    11881 tcagcgacgt agatgcgtac cacctggacc aggcctttcc gctgctgatg aaacaactgg
    11941 aactcatgct cactggcggt gaactgaatc cccgccatca gcataccgtc acactgtatg
    12001 cgaaagggct gacctgcgaa gccgacaccc tcggctcctg tggttacgtt tatctggctg
    12061 tttatccgac accggcagcg cccgcaacca ccgtataaca ccggatatct cccctttata
    12121 accacctgtt acagagaact taagatgaat acattacccg acactcacgt acgggaggca
    12181 tcgggctgcc cgtctcccat caccatctgg cagacactgc tcacccgact gctggaccag
    12241 cactatggcc tcacgctgaa tgacacaccg ttcgctgatg aacgtgtgat tgagcagcat
    12301 attgaggcag gaatttcact gtgtgacgcg gtgaactttc tcgttgaaaa atacgcgctg
    12361 gtacgtaccg accagccggg attcagcgca ggagccccgt cgcagttaat caacagcatt
    12421 gatattctcc gggcacgccg ggcaaccggc ctgatgaccc gcgataacta cagaacggta
    12481 aataacatta cccgggggaa gcatccggaa gcgaaacaat gaaactggcc ctgacgctgg
    12541 aagccgacag cgttaacgtg caggcactga acatggggcg cattgtcgtt gacgtcgatg
    12601 gtgttaatct cagtgaactg attaacaagg tctctgaaaa cggttattca ctccgcgtgg
    12661 tcgataaatc cgaccaacac gcaaccagta caccaccacc gttaacaacc cttacctgca
    12721 tacgttgcag caccgcacat atcacggaaa cggacaacgc ctggctgtac tcgctgtcac
    12781 accagactaa tgacgacggc gaaacagaat ggattcattt tacaggcagc ggctatctgt
    12841 tacgtaccga tgcatggtcg tacccggttc tgcggcttaa acgcctgggc ctgtccagaa
    12901 cgttccgtcg tctggtcgtc actctcaccc ggcgttatgg cgtcagtctc attcatctgg
    12961 acgccggcgc agaatgtctg ccggggttcc ccactttcga ctggtaatca ggacaaaaga
    13021 cactggatga aattattaac cacagaaacg gcgctggata ttctgattgc gtggctgcag
    13081 gacaatattg actgcgaatc agggattatc ttcgacaacg atgaggataa aacggattcg
    13141 gcagcactgc tgccctgtat tgaacaggcc agggaagata tccgtaccct gcgtcagctg
    13201 cagtttctgc aacagaaccg gtgagtctta ctcaccagac ttcattccac tgacgccagc
    13261 ctgaatacgg ctggcgtttt cgtttatctg caaaaaggaa tatcgattat gtctgaaatc
    13321 acagcctctc gtccggaagt ggtcaacggg catacggacg ttatctgctc aaccagtatc
    13381 cgccacattc tggctgtacg aaagagtaca ttgctgcaaa tcgacacact tatccgacaa
    13441 ctggctgaag tatcagcaat gacagaaagt attagaggta aaacggctct ggactgggcc
    13501 atgaaacagg attttcgctg cggttgctgg ctgatggaga aaccggaaac cgcaatgaaa
    13561 gccatcacac gcaatcttga tcgcgaactc tggcgtgatc tgatgcaacg ttccgggatg
    13621 ctgagtctga tggatgcgca gacccgggac acatggtaca ggtcactgga atatgataat
    13681 tttccggaaa tcagcgaagc gaacattctg agcacatttg aacaactgca ccagaacaaa
    13741 gatgaagtgt ttgaacgggg agtgatcaac gtcttcaaag ggctgagctg ggattacaaa
    13801 accaactcac cctgtaaatt tggcagtaaa attatcgtca acaacctggt gaggtgggac
    13861 cggtgggggt ttcatcttat caccgggcaa cagactgacc gactcgtcga cctggaaaga
    13921 atgctacatc tgttcagcgg caaaccgatc cccgacaacc gggaaaacat cactattcgt
    13981 ctggatggtc acatccagtc cgttcagggt aaagagcgct atgaagacga gatgttcatc
    14041 attaaatact ttaagaaggg atctgcacac atcactttca aaaggctgga actgattgac
    14101 agaattaacg atataatagc caggtacttt ccttctgtgc tctcagcctg atcccgagag
    14161 tgattccctt tcgatatcaa aagggactgc gggtataatt gagggtacat ttttaatcaa
    14221 accaaacaaa ttgcattaat atcaatatga taaaagaaat tttcgattcc gagtccggca
    14281 c
//
LOCUS       DAZSNM010000100        14122 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZSNM010000100 DAZSNM010000000
VERSION     DAZSNM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14122
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.100"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..275)
                     /gene="ygiW"
                     /locus_tag="ACHW42_002459"
     CDS             complement(<1..275)
                     /gene="ygiW"
                     /locus_tag="ACHW42_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR8944249.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDH"
     gene            427..1086
                     /gene="qseB"
                     /locus_tag="ACHW42_002460"
     CDS             427..1086
                     /gene="qseB"
                     /locus_tag="ACHW42_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR8944250.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            1083..2357
                     /gene="qseC"
                     /locus_tag="ACHW42_002461"
     CDS             1083..2357
                     /gene="qseC"
                     /locus_tag="ACHW42_002461"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR8944251.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(2403..2735)
                     /locus_tag="ACHW42_002462"
     CDS             complement(2403..2735)
                     /locus_tag="ACHW42_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFR8944252.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            3054..3635
                     /gene="mdaB"
                     /locus_tag="ACHW42_002463"
     CDS             3054..3635
                     /gene="mdaB"
                     /locus_tag="ACHW42_002463"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR8944253.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            3666..3980
                     /locus_tag="ACHW42_002464"
     CDS             3666..3980
                     /locus_tag="ACHW42_002464"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR8944254.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(4028..5920)
                     /gene="parE"
                     /locus_tag="ACHW42_002465"
     CDS             complement(4028..5920)
                     /gene="parE"
                     /locus_tag="ACHW42_002465"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR8944255.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(5949..6530)
                     /gene="yqiA"
                     /locus_tag="ACHW42_002466"
     CDS             complement(5949..6530)
                     /gene="yqiA"
                     /locus_tag="ACHW42_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR8944256.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(6530..7357)
                     /gene="cpdA"
                     /locus_tag="ACHW42_002467"
     CDS             complement(6530..7357)
                     /gene="cpdA"
                     /locus_tag="ACHW42_002467"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR8944257.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(7382..7804)
                     /locus_tag="ACHW42_002468"
     CDS             complement(7382..7804)
                     /locus_tag="ACHW42_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR8944258.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(7805..8434)
                     /gene="nudF"
                     /locus_tag="ACHW42_002469"
     CDS             complement(7805..8434)
                     /gene="nudF"
                     /locus_tag="ACHW42_002469"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR8944259.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            8639..10120
                     /gene="tolC"
                     /locus_tag="ACHW42_002470"
     CDS             8639..10120
                     /gene="tolC"
                     /locus_tag="ACHW42_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR8944260.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            10268..10939
                     /locus_tag="ACHW42_002471"
     CDS             10268..10939
                     /locus_tag="ACHW42_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR8944261.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVLMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            10945..12105
                     /locus_tag="ACHW42_002472"
     CDS             10945..12105
                     /locus_tag="ACHW42_002472"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR8944262.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(12143..12931)
                     /gene="ygiD"
                     /locus_tag="ACHW42_002473"
     CDS             complement(12143..12931)
                     /gene="ygiD"
                     /locus_tag="ACHW42_002473"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR8944263.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            13074..13847
                     /gene="zupT"
                     /locus_tag="ACHW42_002474"
     CDS             13074..13847
                     /gene="zupT"
                     /locus_tag="ACHW42_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR8944264.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(13905..14075)
                     /gene="yqiD"
                     /locus_tag="ACHW42_002475"
     CDS             complement(13905..14075)
                     /gene="yqiD"
                     /locus_tag="ACHW42_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR8944265.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
BASE COUNT         3575 a         3731 c         3484 g         3332 t
ORIGIN      
        1 ttgtgatcga tatcaacatt gatagtaccg ctggcatctt taaacacgta gagatcgtca
       61 gagatgcgtt caacgatatt gccgcgcagg gttacccagg tgtcgtcacg cagggatttt
      121 gcgctttcta cagtcgttac gctgccgttc ggcccctgaa atcctccggc ctgactttgc
      181 gttaccgatg ggccagaaaa accgccctgc tctgctgcca tcaccggtgc actgcacagg
      241 gccattactg cgattactgc tgcgaatttt ttcatgttta ttactccctt taatgtctgt
      301 ttccgagcat ttaacaagat agtccttaac aacttcttaa gggaaaaaaa taaaatttaa
      361 tgctgtacag agcgcgttac aacacggttt actggcagca aatacggtta tcgcagggat
      421 gaaaaaatgc gaattttact gatagaagat gacatgctga ttggtgacgg catcaaaact
      481 ggccttagta aaatgggttt tagtgtcgac tggtttacac aaggtcgtca gggaaaagag
      541 gcgctttata gcgcacctta tgatgcggtg atcctggatt taaccttacc aggcatggat
      601 ggtcgcgata ttttgcgcga atggcgagag aaaggtcagc gtgagccggt actgaacctg
      661 accgcgcgtg atgcgttagc ggaacgtgta gaagggctgc gtctgggagc tgacgattat
      721 ctgtgtaaac cttttgcgtt gatagaagtc gccgccaggc tggaggctct gatgcgccga
      781 accaacggcc aggccagcaa cgagctgcgc cacggtaacg tcatgctcga ccccggcaaa
      841 cgtatcgcca cgctggctgg cgaaccctta acactgaaac caaaagaatt tgccctgctg
      901 gaattactga tgcgtaacgc tggtcgggta ctgccgcgca aactgattga agagaaactg
      961 tatacctggg acgaagaggt caccagtaat gccgttgaag tgcatgtgca tcatctgcga
     1021 cgcaaactcg gtagtgattt tattcgtacc gtgcatggta ttggctacac attaggtgag
     1081 aaatgaaatt tacccaacgt cttagtctgc gcgtcaggct gacgctaatc tttttaattc
     1141 tggcctcggt gacctggctg ctttccagct ttgtcgcctg gaaacaaaca acggataacg
     1201 tcgatgaatt gttcgacacc caactgatgc tgtttgccaa acggttaagt acgctcgatc
     1261 tcaacgaaat caacgcggcg gatcgcatgg cacagacgcc aaataaatta aaacacggtc
     1321 atgttgatga cgatgcgctg acctttgcca tctttaccca cgacggcaga atggtcctta
     1381 atgatggcga taacggagaa gatattccct atagctatca acgggaaggt tttgctgacg
     1441 ggcaactggt cggtgaagac gatccttggc gttttgtctg gatgacctca cctgatggca
     1501 aatatcgcat cgttgttggc caggaatggg aataccgtga agacatggcg ctggcgattg
     1561 ttgccgggca attgatcccg tggctggtcg cactgccgat tatgttaatc atcatgatgg
     1621 tactactggg tcgtgaactc gcgccgctga acaaactggc gctggcacta cgtatgcgtg
     1681 accctgactc ggaaaaacca ctaaacgcga ctggcgtacc cagcgaagtg cggccactgg
     1741 ttgagtcgct aaatcaactg ttcgcccgca cacatgcgat gatggttcga gaacgacgct
     1801 ttacctccga tgcagctcac gaactacgta gcccgttaac agcgctgaaa gtgcaaaccg
     1861 aagttgcgca gctctctgac gatgatccgc aggcgcggaa aaaagcactg ctccaattac
     1921 attccgggat cgatcgcgct actcgtctgg ttgatcaact gctcacgcta tcgcggctgg
     1981 actcactgga taaccttcag gacgtcgcgg agatcccgct tgaagatctc ctacaatcgt
     2041 cggtgatgga tatttaccac acggcgcagc aggcgaaaat tgacgtgcga ctgacactca
     2101 atgcccacgg catcaaacgc accggacaac cgctattgct aagtttgttg gtgcgaaatt
     2161 tgctggataa cggccccggt gtgacaccag aggcactggc gcgaattggc gaacgcttct
     2221 atcgcccacc cggacaaacc gctaccggca gtggacttgg gctatcgatt gtccagcgaa
     2281 tcgccaagct gcatgacatg aatgttgagt ttgggaatgc ggaacaaggt ggatttgagg
     2341 cgaaggtaag ctggtaactt ctctgtctat gagagccgtt aaaacgactc tcatagattt
     2401 tactaatagc aaaatataaa ccgtccccaa aaaagccacc aaccacaaac cagacaaata
     2461 acgagcgaga atacatatag ccaaaactcg aacatttccc gcataaagag ttttcttaag
     2521 ataagaataa taagtggcgt aagaagaaaa aatgctgcaa tcattgcttg caatatacga
     2581 tcttcgcctg aaacggaagg tatactgcaa atattgcctc ccgactcaag catccagtca
     2641 tacttattga aagcaaaaaa agaatctatt aaccaataag taagcattat atatggcaat
     2701 gcttcaaata ttgttttaat tttctgcttt aacatatatc agtacgctca taaaaatcca
     2761 tttataatta tcaacatgac acgttgatat tttcatcttt tacttattca aagccatatt
     2821 ttcaatcccc aaaaaaaccg aaccaaacgt gtaaattatt ccggtgcgag taagaattat
     2881 gaggaatggc tatcagtatt gtcattttca gaaaatattt atcctgcatc ggtgagtcag
     2941 agctagatca gacttttgct aaattcgcaa aagactttgc acattttgct aatttcacag
     3001 taccgctctg tgacgtacta tagtcgacaa acgtctcacc ttgagattaa aaaatgagca
     3061 acatcctgat tatcaacggc gcgaaaaaat tcgcccactc caatggtcaa ctgaacgaca
     3121 ccctgaccga agtcgcggat ggcacactgc gcgacctcgg gcatgatgtc cgcatcgttc
     3181 gcgccgacag cgactacaat gtcaaagcgg aagtacaaaa ctttctctgg gctgatgtgg
     3241 tgatctggca gatgccaggc tggtggatgg gcgcgccgtg gacagtgaaa aaatatattg
     3301 atgatgtatt caccgaaggt cacgggactc tgtatgccag cgatggtcgt acccgcaaag
     3361 atccgtcgaa aaaatacggt tccggcagcc tggtacagga caaaaaatat atgctttctc
     3421 taacctggaa cgcaccaatg gaagccttca ccgaaaaaga tcagttcttc cacggcgttg
     3481 gcgttgacgg tgtgtatctg ccgttccata aagcaaacca attcctcggt atggaaccgc
     3541 tgccgacatt tatcgctaat gacgtgataa aaatgcctga tgttccccgc tatactgaag
     3601 aatatcgcaa gcatcttgtg gaaatttttg gttaactaga gctcaggctt tagaaggagt
     3661 taaccatgct taccgtaatc gcagaaatcc gtactcgtcc tggtcaacat caccgtcagg
     3721 cggtattgga tcagtttgct aaaatcgttc caaccgtact gaaagaagaa ggttgccacg
     3781 gctatgcgcc aatggtggat tgcgctgctg gcgtgagttt ccagtctatg gcaccggatt
     3841 ctatcgtgat gattgagcag tgggaaagca tcgcgcacct tgaagcgcat ctgcaaaccc
     3901 cgcacatgaa ggcgtacagc gaagccgtaa aaggcgacgt gctggagatg aatatccgta
     3961 ttctgcagcc agggatttaa tcctgccttg tttgcccggc catcctgacc gggcaatgtt
     4021 ctttccttta aacctcaatc tccgccatgt cgcctttctc ttgcaaccag ttgcggcgat
     4081 cttccgagcg tttcttcgcc agcagcatat ccatcatcgc gtcagtacgc tgatcgtctt
     4141 catcatcgat agtcaactgc accagacggc gagtgttcgg atcaagcgtg gtttcacgca
     4201 attgcatcgg gttcatttcc cccagacctt taaaacgctg gacgttcggc ttgcctttct
     4261 tgcgttttaa ttgctcaagt acgccctctt tctcttcttc cgtcagcgcg taataaacct
     4321 ctttcccgag atcaatacgg tagagcggtg gcagtgcgac gtaaacgtga ccgtgtttca
     4381 ccaacgcgcg gaagtgtttt acgaacaaag cgcagagcag cgtggcaatg tgcagaccat
     4441 cagagtccgc atccgcgagg atacagattt tgccataacg aagctggctc agatcgtcgc
     4501 tgtcaggatc gataccgatc gctaccgaaa tatcatgcac ttcctgcgag gccagcactt
     4561 cgtcggaaga aacttcccag gtgttaagga tcttaccttt cagcggcatg atcgcctgat
     4621 attcacgatc gcgcgcctgc ttggcagatc cgcctgcgga gtcaccttcc acgaggaaca
     4681 gttcggtacg gttaaggccc tgcgcggtac aatcagccag tttgccaggc agcgccgggc
     4741 cgctggtcag ctttttacgc accacttttt tagccgcacg catacgacgc tgggcgctgg
     4801 aaatcgccat ctctgccagc aactccgccg cctgaacgtt ctggttcagc cacaggatga
     4861 aggcatcttt caccacgccc gaaacgaatg ccgcacactg acgcgaagag agacgctctt
     4921 tggtctgccc ggcaaactgc ggatcctgca ttttaaccga cagaacatag gcgcagcgat
     4981 cccagatatc ttccgccgac agcttcacgc cgcgcggcag aatgttgcgg tattcacaga
     5041 actcacgcat cgcgtccaac aggccctgac gcagaccatt aacatgggta ccgccctgca
     5101 tcgttgggat aaggttgacg tagctttcgg tcagcagttc accgccttcc ggcagccaca
     5161 gtagcgccca gtccacagct tcagtatcgc cagcgaaatt accgataaac ggtttttctg
     5221 gcagcgtcgg taaaccgttt accgcttccg ccaggtaatc attcagaccg tcctgatagc
     5281 accagcgctg ttcggtgttg ttgatctcat ctttaaaggt aatctcaacg ccggggcaca
     5341 gtactgcttt ggccttcagc acatgcgtca ggcgtgaaac agaaaaacgc gggctgtcaa
     5401 agaaggtttc atccggccag aagtgcacgc tggtaccggt attgcgttta ccgcaagtgc
     5461 cgacaacctg taaatcctgc accttttcgc cattttcaaa ggcgatgtta taaacctgac
     5521 catcgcggcg cacgttaact tctacgcgct tcgacaaggc gttaactacc gaaatcccca
     5581 cgccatgcag gccgccagag aactggtaat ttttgttaga gaatttaccg cctgcatgca
     5641 gacggcaaag aatcagttca accgccggta ctccttcttc cgggtgaata tccaccggca
     5701 tcccgcgccc atcgtcaatc acttctaacg actggtcagc atgtaagatt acatccacgc
     5761 gttttgcgtg acccgccagt gcttcatcga cactgttatc aataacttct tgccccaaat
     5821 ggttagggcg agtggtatcg gtatacatcc ccggacggcg gcgaaccggc tcaagcccgg
     5881 tgagtacctc aatggcatca gcgttataag tttgcgtcat ggtttaagtt agcaattcga
     5941 gttgatcgtc agagatggtg cagaccaaga aaatcgacga tcgggttgaa ataatcttcg
     6001 aagcccgtga atgcgtggtt gccgccttct atgacagtct ggcggcagga agcatagtac
     6061 gccaccgcct ggcggtaatc cagcacttca tctcccgtct gttgcagcag ccagatcaaa
     6121 tccggcgctt ccagcgggtc aatctgcatg actttgagat cgtaaatatg gcgtgactct
     6181 agcacatatt gctgcccggt gtaggggttc tcgttctgac cgagatagtc cgtcagcaat
     6241 tcaaacgggc gtaccgccgg gtttaccacc actgcgggca gcataaaaca ttgcgacaac
     6301 caggtggcgt aatatccccc cagtgacgaa ccgacaatac ccagcgaatc gccgccatgt
     6361 tccaggacaa tggattccag cagttctgcc gcgtcggaag gatacggcgg caactgcgga
     6421 atgatcatct caacgtcagg gtgatgttcc gccagccagt tttttaacaa gctcgctttt
     6481 gcagagcgcg gcgagctgtt gaaaccgtgt aaataaagaa gcgtagacat cagtagcctt
     6541 ctgaagcggt atcaggttgg aaacgtgtgt ccgccaggcg atgcacctcg gtggtcagcg
     6601 tgccatcagc atgtaactcg agagtacgcc agccgggcgc gatagtatcc agcgtaaagt
     6661 tggaacagtg cggcttaaac tgcacacagg tcgacggcgt tgccagcagg cggcgaccgt
     6721 tccagtcgag atccagctcc tgatgaatat gaccgcacag caagtatttg acgtgcggaa
     6781 acttcgccag cacggtatcc agttcgcccg cgttacgcag actgtgttga tcgagccaac
     6841 tacaacccgc aggtagcgga tgatgatgca gcagcagcaa cgtatggcgt tctggcgcat
     6901 cggccagttt acgttccagc cactcaagct gaaactcgct cagctcaccg tgcggcacgc
     6961 caaacacctg gctatccagc aacaggattt gccattgctc accaataaac acccgcttcg
     7021 ccggggagat acccgcatcc tgtaacgcgc tgtacatcgc gggctggaaa tcgtggttgc
     7081 ccggcagcca gacgcagggc gcacgaaaac ttgcgatgcc ttcagcgaaa tgctgatagg
     7141 ccgcagagga ttgatcctgc gctaaatcac ctgtcgcgac aatcaggtcg aattcgtgct
     7201 ggtgtggacg aatcgcctcc agcaccgcct ggtagctttc ccaggtgttt acccctaaca
     7261 gggcttcgtg cttttgtgca aacaggtgag tatcggtaat ttgtaaaatc cttcctctgg
     7321 cctcaccagc cagaggaagg gttaacaggc tttccaaatg gtgtccttag gtttcacgac
     7381 gctagtaaac cggaatcgcc atcgctccat gtgctaaaca gtatcgcaac cagtccgcta
     7441 aaaactggtt aatttgatgc ttttcgtcgc gttgatgcaa ctttttatta ggataatcat
     7501 accgcgcttt gaagcgaaaa atctgctggc ttgaacacac ttcagccacc atcgcgtcat
     7561 gatacagacg caccgtcatt gacggaaggc tccagtaact gatcgcgggc gcagtctgtt
     7621 ctattgtcac cagggtagtg tatcgggtcg attccacaat cgtcagccga tattgtgcgt
     7681 ttgccacctg atagcttaca gtttcgccgg gtgcgtcatt gcgcggtaac aaacggcgca
     7741 attgtgaaaa gttcatctcg cacaggcgca tcatttcagg aaagtcaggt gtgtaacgct
     7801 tcatttatgc ccactcattt tttaacgctt gatgatgcag ctgcagccat tgcaaagcga
     7861 tgaccgacgc tgcgttgtcg attttcccct cttctaccca ctggtatgcc tgttcccggc
     7921 ttaccacatg aacgcgaata tcttcgtttt catcagccag accatgaata ccgcttgcgg
     7981 tcgtggcgtc cacttcgccc accataattg acgaacgctc actggtgccc cccgggcttg
     8041 ccaggaaact taacaccggt ttggtccgtt tgactatcag tcccgcctct tcaatcgctt
     8101 cgcgacgggc aacatcttcc acactttcac cctcttcaat catcccggca accatctcca
     8161 gtagccaggg ggtttcgctg gtgtcgtacg cggcaatccg aatctgctca atcagcacaa
     8221 cttcatcacg cactgggtca aagggtagca agactgcggc gtgaccgcgc tcaaaaattt
     8281 cccgccgtac ctcatgactc atttgcccgt tgaatagacg atgacgaaat ctataaagat
     8341 ctaatgaaaa aaagccgcga taaagtgttt ctcgtgcaat aatttctaca tcgtttttgc
     8401 caaatgtaac gggcaggttg tctggcttaa gcattgttaa tgtcctagca ctaatagtga
     8461 attaaatgtg aatttcagcg acgtttgact gccgtttgag cagtcatatg ttaaattgag
     8521 gcacattaac gccctatggc acgtaacgcc aaccttttgc ggtagcggct tctgctagaa
     8581 tccgcaataa ttttacagtt tgatcgcgct aaatactgct tcaccacaag gaatgcaaat
     8641 gaagaaattg ctccccattc ttatcggcct gagcctttct gggttcagtt cgttgagcca
     8701 ggccgagaac ctgatgcaag tttatcagca agcacgcctt agtaacccgg aattgcgtaa
     8761 gtctgccgcc gatcgtgatg ctgcctttga aaaaattaat gaagcgcgca gtccattact
     8821 gccacagcta ggtttaggtg cagattacac ctatagcaac ggctaccgcg acgcgaacgg
     8881 catcaactcg aacgcgacca gtgcgtccct gcagttaact caatccattt ttgatatgtc
     8941 gaaatggcgt gcgttaacgc tgcaggaaaa agcagcaggg attcaggacg tcacatatca
     9001 gaccgatcag caaaccttga tcctcaacac cgcgaccgct tatttcaacg tgttgaatgc
     9061 tattgacgtt ctttcctata cacaggcaca aaaagaagcg atctaccgtc aattagatca
     9121 aaccacccaa cgttttaacg tgggcctggt agcgatcacc gacgtgcaga acgcccgcgc
     9181 gcagtacgat accgtgctgg cgaacgaagt gaccgcacgt aataaccttg ataacgcggt
     9241 agagcagctg cgccagatca ccggtaacta ctatccggaa ctggcggcgc tgaatgtcga
     9301 aaactttaaa accgacaaac cacagccggt taacgcgctg ctgaaagaag ccgaaaaacg
     9361 caacctgtcg ctgttacagg cacgcttgag ccaggacctg gagcgcgagc aaattcgcca
     9421 ggcgcaggat ggtcacttac cgactctgga tttaacggct tctaccggga tttctgacac
     9481 ctcttatagc ggttcgaaaa cccgtggtgc cgctggtacc cagtatgacg atagcaatat
     9541 gggccagaac aaagttggcc tgagcttctc gctgccgatt tatcagggcg gaatggttaa
     9601 ctcgcaggtg aaacaggcac agtacaactt tgttggtgcc agtgagcaac tggaaagcgc
     9661 acatcgtagc gtcgtgcaaa ccgtacgttc ctccttcaac aacattaatg cttctatcag
     9721 tagtattaac gcctacaaac aagccgtagt ttccgcgcaa agctcattag acgcgatgga
     9781 agcgggctac tcggtcggta cgcgtaccat tgttgatgtg ttggatgcga ccaccacgct
     9841 gtacaacgcc aagcaagagc tggcgaatgc gcgttataac tacctgatta atcagctgaa
     9901 tattaagtca gccctgggta cgttgaacga gcaggatttg ctggcactga acaatgcgct
     9961 gagcaaaccg gtttccacta atccggaaaa cgttgccccg caaacgccgg aacagaatgc
    10021 tattgctgat ggttatgcgc ctgatagccc ggcacccgtc gttcagcaaa catccgcacg
    10081 cactaccacc agtaacggtc ataacccttt ccgtaactga tgacgacgac ggggcttcgg
    10141 ccccgtctga acgtaaggca acgtaaagat acgggttatc tgccgcattc ttcccccttc
    10201 tcgcttcaat ttcgaccagc catcctctat tctgatgggt atttaccact ggtcccggaa
    10261 gacaaaaatg aaacggacaa aatccatacg ccacgcatcg ttccgcaaaa actggagcgc
    10321 acgccatctg acaccagtcg ctctcgcggt tgccactgtt cttatgctgg ctggctgtga
    10381 aaagagtgat gaaacagtgt ctctctatca aaatgctgac gactgttcag ctgcaaaccc
    10441 aggcaaaagc gccgaatgta ccaccgcgta caacaatgcg ctgaaagaag ccgaacgtac
    10501 tgcgccgaaa tacgccaccc gtgaagactg tgttgctgaa tttggtgaag gtcagtgtca
    10561 gcaagcacca gcccaggctg gcatggcacc agaaaaccag gcgcaggctc agcaatccag
    10621 cgggagtttc tggatgccgc tgatggccgg ttacatgatg gggcgtctga tgggcggcgg
    10681 cgcgggattt gcacagcagc cgctgttctc ctcgaaaaac ccggccagtc cggcttacgg
    10741 taaatatacc gacgcgacgg gtaaaaacta tggcgcagcc cagccaggcc gcaccatgac
    10801 cgtaccgaag acggcaatgg caccaaaacc ggcgaccacc actaccgtta cccgtggcgg
    10861 ttttggtgaa tctgttgcca aacaaagcac tatgcagcgt agcgcaaccg gcacctcttc
    10921 tcgttcaatg ggtggctgat accgatggaa agagtcagta ttaccgagcg cccggactgg
    10981 cgtgagaaag cccacgaata cggtttcaat tttcacacca tgtacggcga gccgtactgg
    11041 tgtgaagatg cttactacaa gttgaccctc gcccaggttg aaaagctgga agaagtcacc
    11101 gccgaactgc accagatgtg cctgaaagtg gtggaaaaag tgatcgccag cgatgagctg
    11161 atgaccaaat tccgcatacc aaaacacacc tggagttttg tgcgtcaatc atggctgact
    11221 caccagccat cgctttattc gcgtcttgat ctggcgtggg atggcactgg cgaacctaaa
    11281 cttctggaaa ataacgccga tacgccaacg tcactatacg aggcggcgtt ctttcagtgg
    11341 atctggctgg aagatcagct taacgccggt aacttgccgg agggcagcga ccagtttaac
    11401 agtctgcaag aaaaactgat cgatcgcttc gttgagctgc gtgaacagta tggcttccag
    11461 ttgctgcatc tcacctgctg tcgcgacacg gtggaagatc gcggaaccat tcagtatttg
    11521 caggactgcg caaccgaagc tgaaatcgcc accgagttcc tctacatcga cgagataggt
    11581 ttaggcgaaa aaggccagtt cacggattta caggatcagg tgatttctaa cctgttcaag
    11641 ctttatccgt gggaatttat gctgcgcgaa atgttctcca ccaagctgga agatgctggc
    11701 gtacgctggc tggaaccggc gtggaagagc attatctcca acaaggcact tctaccgcta
    11761 ctgtgggaga tgttcccgaa tcacccgaac ctgctgcccg cttattttgc ggaagatgat
    11821 catccgcaaa tggaaaaata tgtggttaaa ccgatcttct cccgtgaagg cgcaaacgtg
    11881 tcgatcattg agaacggcaa aaccattgaa gcagcggaag gtccgtatgg cgaagaaggg
    11941 atgattgttc agcaattcca cccgttaccg aaattcggcg acagctatat gctgattggt
    12001 agctggctgg tgaacgatca acctgccgga attggcattc gtgaagaccg tgcgttgatc
    12061 acccaggata tgtctcggtt ttatccacat atttttgttg aataagccac gataccggat
    12121 ggcactcgcc atccggtaat tgttagccta tctgcaccga cagcatactt aaactgccca
    12181 tttctatacc atcgaccgga atggtaattg gctcctgccc atcccacaca cctaacacat
    12241 acaacaacgg cagatagtgc tctggcgttg ggttcgataa cgcgccacct tcatggtcga
    12301 gataattcac cagaggatgc tgctctaccg gcccttgcca cgtcagattc gctttcacat
    12361 actcattaaa cgacgtcgcc cacggatacg gtgaactatc accgtgccac ttcactgtgc
    12421 gcaggttatg caccacgtta ccgctggcga ccaacattat tccttcatca cgcagcgccg
    12481 cgagtttgcg ccccatttcg aaatgccagg cggcaggttt gctactgtcg atactcaact
    12541 gcaccatagg gatatcggcg tcaggataca tcttaatcag cacgccccac gagccgtggt
    12601 caaagcccca ggcttcttta tccagcgtca ccgggatcgg cgctaacagc tcaaccagac
    12661 gctgtgccag cgcaggcgaa cccggagcag gataatgcgt atcgtacagc gcctgcggga
    12721 agccaccaaa gtcatgaatc gtgggcggcg tctccatcgc ggtcactcct gttccacggg
    12781 taaaccagtg agccgaaacc accacaatcg cttgcgggcg tggcaatgtc atccccaact
    12841 tctgccagct gcgggtatac aaattatctt ccagcacgtt catcggacta ccgtgaccta
    12901 aaaacaatgc tggcatacgt gttgaagaca tgatgatatc cttaactaaa ggtgtcattt
    12961 tgatatcctc acaatacgct tgttcggcgg agtaagaacc cggataacaa tgatgatgat
    13021 catcagttat tttgacgatc tgcctgaagg tgaagattta taaggagttg tcgatgtcag
    13081 tacctctcat tctgaccata ctggcggggg cagccacgtt tattggcgcg tttctcggcg
    13141 ttctcggaca aaaaccctcg aaccgcttac tggcgttttc gctaggtttt gcggcgggga
    13201 tcatgttgct catctcatta atggaaatgc ttcctgccgc actagcagct gaaggaatgt
    13261 cgccggtgtt gggttatgga atgtttatct tcggtctgct tggctacttt ggcctggacc
    13321 gcatgttgcc gcatgcgcat ccgcaggatt taatgcaaaa atcggtgcag ctgttgccaa
    13381 aatcgatcaa gcgcacagcc attctgctca ctctcggcat cagtctgcat aacttcccgg
    13441 aagggattgc cacctttgtc acggcgagca gcaacctgga gctgggattt ggcatcgcac
    13501 tggccgtcgc gttgcacaat atccctgaag gtctggcagt ggcaggcccg gtttatgcgg
    13561 caacggggtc taaacgcacc gcgattctgt gggcggggat ttctggacta gcagaaattc
    13621 ttggtggtgt gctggcgtgg ttaatcctcg gtagcatgat ttccccggtg gtcatggcgg
    13681 caatcatggc ggcggttgca ggaattatgg tggcgctctc ggttgatgaa ttaatgccgc
    13741 tcgccaaaga gattgaccct aataataacc ccagctatgg cgtactatgt ggaatgtcag
    13801 tgatgggatt cagtttagtg ctgctacaaa cggcgggaat tggttaaaaa caacatcaga
    13861 agttgtccag cacatggaca acttctgcca gcttcacgaa agccttattc taccttttcg
    13921 gctgtctctt ctctcgtact gtttaagtat ttgttccgcg cttcaagcag tgcgtctctg
    13981 tcctggcgaa acgcccgaca ataacgtttc aaatgcagga aataacccac cacaaccaga
    14041 gcaatcaata caatccagta ccaggcgata aacatccttt tacccttaat atttaacaat
    14101 actaaacatt tagcgcataa at
//
LOCUS       DAZSNM010000101        14015 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZSNM010000101 DAZSNM010000000
VERSION     DAZSNM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14015
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.101"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="ACHW42_002476"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="ACHW42_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR8944266.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="ACHW42_002477"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="ACHW42_002477"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR8944267.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="ACHW42_002478"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="ACHW42_002478"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR8944268.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="ACHW42_002479"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="ACHW42_002479"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR8944269.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="ACHW42_002480"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="ACHW42_002480"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR8944270.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="ACHW42_002481"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="ACHW42_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR8944271.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="ACHW42_002482"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="ACHW42_002482"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR8944272.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="ACHW42_002483"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="ACHW42_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="ACHW42_002484"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="ACHW42_002484"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR8944273.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="ACHW42_002485"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="ACHW42_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR8944274.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="ACHW42_002486"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="ACHW42_002486"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR8944275.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="ACHW42_002487"
     CDS             complement(13538..13933)
                     /locus_tag="ACHW42_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR8944276.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..>14015)
                     /locus_tag="ACHW42_002488"
     CDS             complement(13952..>14015)
                     /locus_tag="ACHW42_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944277.1"
                     /translation="QWILAKAAAEQEKFRFRYEQ"
BASE COUNT         3425 a         3677 c         3427 g         3486 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 cgggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gacaaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagccagg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttctaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggct gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatggcgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccgaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgttggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgccgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgaat
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat ggcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attgg
//
LOCUS       DAZSNM010000102        13946 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZSNM010000102 DAZSNM010000000
VERSION     DAZSNM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13946
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.102"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(184..936)
                     /gene="tcyN"
                     /locus_tag="ACHW42_002489"
     CDS             complement(184..936)
                     /gene="tcyN"
                     /locus_tag="ACHW42_002489"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR8944278.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(933..1601)
                     /gene="tcyL"
                     /locus_tag="ACHW42_002490"
     CDS             complement(933..1601)
                     /gene="tcyL"
                     /locus_tag="ACHW42_002490"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR8944279.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1616..2602)
                     /gene="dcyD"
                     /locus_tag="ACHW42_002491"
     CDS             complement(1616..2602)
                     /gene="dcyD"
                     /locus_tag="ACHW42_002491"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR8944280.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2707..3507)
                     /gene="tcyJ"
                     /locus_tag="ACHW42_002492"
     CDS             complement(2707..3507)
                     /gene="tcyJ"
                     /locus_tag="ACHW42_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8944281.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3595..4146)
                     /gene="fliZ"
                     /locus_tag="ACHW42_002493"
     CDS             complement(3595..4146)
                     /gene="fliZ"
                     /locus_tag="ACHW42_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR8944282.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4192..4911)
                     /gene="fliA"
                     /locus_tag="ACHW42_002494"
                     /pseudo
     CDS             complement(4192..4911)
                     /gene="fliA"
                     /locus_tag="ACHW42_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(5076..6728)
                     /gene="fliC"
                     /locus_tag="ACHW42_002495"
     CDS             complement(5076..6728)
                     /gene="fliC"
                     /locus_tag="ACHW42_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFR8944283.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            6994..8400
                     /gene="fliD"
                     /locus_tag="ACHW42_002496"
     CDS             6994..8400
                     /gene="fliD"
                     /locus_tag="ACHW42_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR8944284.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8425..8835
                     /gene="fliS"
                     /locus_tag="ACHW42_002497"
     CDS             8425..8835
                     /gene="fliS"
                     /locus_tag="ACHW42_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR8944285.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            8835..9200
                     /gene="fliT"
                     /locus_tag="ACHW42_002498"
     CDS             8835..9200
                     /gene="fliT"
                     /locus_tag="ACHW42_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR8944286.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9278..10765
                     /gene="amyA"
                     /locus_tag="ACHW42_002499"
     CDS             9278..10765
                     /gene="amyA"
                     /locus_tag="ACHW42_002499"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR8944287.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10799..11212)
                     /gene="yedD"
                     /locus_tag="ACHW42_002500"
     CDS             complement(10799..11212)
                     /gene="yedD"
                     /locus_tag="ACHW42_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR8944288.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11399..12604
                     /gene="yedE"
                     /locus_tag="ACHW42_002501"
     CDS             11399..12604
                     /gene="yedE"
                     /locus_tag="ACHW42_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR8944289.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            12601..12834
                     /gene="yedF"
                     /locus_tag="ACHW42_002502"
     CDS             12601..12834
                     /gene="yedF"
                     /locus_tag="ACHW42_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR8944290.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12941..13611
                     /locus_tag="ACHW42_002503"
                     /pseudo
     CDS             12941..13611
                     /locus_tag="ACHW42_002503"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(13651..>13946)
                     /locus_tag="ACHW42_002504"
     CDS             complement(13651..>13946)
                     /locus_tag="ACHW42_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR8944291.1"
                     /translation="EMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQS
                     KFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3381 a         3629 c         3467 g         3469 t
ORIGIN      
        1 ttcaaactat cacataaata agatttatat ataatttata ttattcaggc aatgaattac
       61 ttttgcaagc catcgcattc tcttatgtta ttaatgagtt atgctgattt gttaagcagt
      121 tttatcacgc ttgaaatggc gtcccgcccc gacaggtgaa tcgtcggggc tgattttttc
      181 ttattattgc agcagaagct tctcgaggaa ctggcgggtg cgaggctgct ggggatcggc
      241 aaataacgct tttgctgccc cctgctcgac tatccgcccc tggtccataa agatcgcccg
      301 gtccgcaaca tcccgggcaa agctcatttc gtgtgtcaca atcaccatcg tgcgcttttc
      361 ctgcgccagc tggcggatgg tgttcaggac ttcgcccacc agctctggat ccagcgccga
      421 cgttggctcg tcaaacaaaa tcacctcagg acgcattgcc agtgcacgcg caatcgcaac
      481 acgctgctgt tgaccgccag acaaacgacg tggatagctg gtttctttac cggccagccc
      541 aactttcgcc agcagctcgc gagcgcgcgc cgtggcctct tctttcggtt cgcctttgac
      601 gatcaccggc ccttcaataa tgttttccag caccgtacga tgcggaaaca aattaaagtt
      661 ctggaagaca aacccgacgt gctgacgcaa ctggcgaatc agcgattttt gctgacttaa
      721 tgaacgtgca gtatcaatag tgatatcgcc aacggtgatc gttcccgctt ccggttgttc
      781 cagcagattt atgctacgta gcaatgtggt tttgccggaa ccactcggac cgataattgc
      841 caccacttcg ccaggcttta cctcgaggtc gataccgtgc agcaccgtct gaccgtggaa
      901 ttttttcacc aggttcttaa cttcaatggc actcattttg gttctctctc ctggcgatta
      961 agttgattct caaaatggtt ctgcaacgtc gataacacag tcgccatgat ccagtagatg
     1021 agcgaagccg ccaggtacat ggtgaacacc tccagcgtgc gcgaggtaat caactgcgcc
     1081 tgacggaaca gctccggcac ctggattgtc gcggccagcg acgtatcttt taccagactg
     1141 ataaagctgt tcgacagcgg cggcaacgcc acccgcgccg cctgtggcaa aatcgcgcga
     1201 cgcatggtct gccacggcgt cataccaata ctggcagcgg cttcccactg acctttatca
     1261 atagaagaaa tggcggcgcg cagcgtttcg gcggcatagg cggcagtgtt cagcgacaga
     1321 ccaatcattg ctgacggaat gggatctaat tcgataccaa actgcggcag gccgtaatag
     1381 atcataaaca gttgtgcgat gagcggcgta ccacgaaaaa tagagatata aaaacgcgcc
     1441 agccagcgca cgggccagat tggcgacagg cgcatcagcg cgagaataaa cccgagtagt
     1501 aagccaaaaa acatgccgcc gatactgagt tgcagggtat acccagcccc tttcaacagg
     1561 aacggcaagg aatcaataac cagttgtata ctttcttgca tgagcgttcc cggagctaaa
     1621 cgtggggatg ataggcgaac agcgcaggcg cgccgccggt atgaataaac agaatcggcc
     1681 cttcatcttt gaagcgtttc tgactgatac cgtcaatcag ccccgccatc gcttttccgg
     1741 tatacacagg atcaagcaga atgccttcca gacgcgccag caatttcact gcttccatgc
     1801 cttcgtcgtt cggcacgccg tagccaggtg caaaatagtc atcccagagt aaaatttccg
     1861 ctgatgcggt cagctccagt tctttcgcaa tcgcctgttg taggttaacc actttcggca
     1921 attgatcggc aacggaacgc gacacggtca cgccaatcag ttcgctttca ggcatcaggt
     1981 gttccagccc aacagccagt ccggcgtgag ttccggcact gcccgatgcg actaccaccg
     2041 acgaaatatt aaccgcccct tcacactgtt gcgcgatttc cagcgcactc tccacataac
     2101 ctagcgcgcc aagagcatta gaaccgccaa ccggaatgac atacgggcga aagccttgtg
     2161 cttcgactcg cgtcgccagt tcttccagtt gggcattggg atcggtcagt gcgtcgcaca
     2221 tttcaatctg ggtattgaac agatccagca acaaacgatt gccgttggtt aaatagtttt
     2281 ctgcggttgt gccaatagga ttttccagca gcgccacgca gtgcagaccg agtttcgccg
     2341 cgactgcggc agtctggcgc acatggttag actggatcgc gccggcagta atcagcgtat
     2401 cggcaccttc gcgcagggca tctgccgcga gaaattccag cttacgtaat ttattgccgc
     2461 ccattgccat gggggtgacc tcatcccgtt tgatgaaaat ttcccgtcca agataatcag
     2521 aaaagcgcgg cagatattcg agcggcgttg gcgcgccgat aaactccaac cgtggaaaac
     2581 gggttaaatt atgcagtggc ataacagcct ccgatgtgtg ttgttgtgat tttcttatta
     2641 tgcacgctga aaacgcgtaa ataaaaaagg cgctagtgaa agcgcccttt tttgtcatta
     2701 tgctgattat ttggtcacat cagcaccaaa ccatttttcg gaaagagctt gcagagtgcc
     2761 gtctttttgc atttccgcaa ttgcatcatt caccgctttc agcaggtctt catttccttt
     2821 acgcagcgcc acgccagact cctgacggga gaatgcttca ccggttactg ccagcgtatc
     2881 gttggttttc ttcaccagat ccagcgccgc cagacgatca acgaggatcg catcgatacg
     2941 ccctacgcgc agatcctgat atttggtcgg gtcatcatca taggtacgca catcgacacc
     3001 ctgaacattc tgccgcagcc actcttcata gttggtgccc agaccgacac ccactttttt
     3061 gcctttcaga tcagcagccg ttttaatggt gccttcgtta ccttttttca ccagcgcctg
     3121 aataccggaa atggtgtaag gggtagagaa atcgtatttt ttcttgcgct cgtcagaaat
     3181 ggtgacctga ttaatcacca catcaatgcg tttagagtcc agcgacgcca gcataccgtc
     3241 ccatttggtc ggttttagtg acgcctcaac gccaagatgt tttgccagct gttgggcaaa
     3301 ttccacttca aaaccggtta atttgccgtc atccccctga aaactgaacg gcggataagt
     3361 gccttccagc cctaccagca gcgtgccgct ctctttgact ttattaagca gaccttcatc
     3421 tgcaaaactt ttaacgctca tgcccgcaac cagcgccacg gccatcacac ccatcaatgc
     3481 ctgacgtccc agatgtgcta atttcatatt caccccgaat gttgttatgt ctgtttgcag
     3541 tgtagagcca ttgtttgtaa acacaaaaac aactccgcta catcttattc ttatttaata
     3601 tatatcagaa gcaggcaggc tggaggattt ctgcacaagt ccggtacagg tttggcgctg
     3661 atagtgttga tacttccgta atgcgatgcg gtaattgttg gtgctggtgg tgggcagcca
     3721 cggggcgagg atttcatcaa ggaaaccgtc ctgcagctga tcgagggaaa tattttgctc
     3781 atgtagatga ttacccaaac ggcgcagacg aacgacatat tcacgcaccg tacctgggct
     3841 catttcagtt tgctcaaaaa gaaatttctt aaagccgata atatcgaaaa aatcactctg
     3901 cgttttgcaa tgtaaatcac cgcaaaatcg gcacaatgcc gcccatgatt gttgttccgt
     3961 ttgccagcct ttttcatcaa gcagcgtgtc caggcgggaa atctcgattt tattcactat
     4021 tttgccgtcg cgaactaagg taatgcgatc gagtaattta cggcaatgcg cgcaatgggt
     4081 ctggctgtgt ttaaagtctt taagatagcg gcttaatggc cgtcttttca ggtgctgcac
     4141 catcattaag aactcctggt agtcaaagtt aaagtgcggc atttactgac gttataactt
     4201 acccagttta gtgcgtaacc gtttaatgtc ctggctgtgt aactgactga cccgcgattc
     4261 cccgacctcc agcaccgcgc caatctcttt gagattcagc tcttcctggt aatagagggt
     4321 taataccagt ttttcgcgct ccggcaacgt ttcgatggct tccatcaccc gctggcgcag
     4381 attactgtcc agcagttgtt gtagcgggtt ttctcgctga tgatcatcag taaccagttc
     4441 gatgctatcg ccgtgctctt cacgccactc atcgtaggag aagagctggc tgttattggt
     4501 gtcgagcagc atttgacgat aatcggcaat atcgatccct aaacgttccg ctacctcagt
     4561 ttccgtggcg ttgcggccaa gttcctgctc cagttgccct attgcctgtg ccacttcacg
     4621 cgcgttgcgt cgcacgctgc gcggcaccca gtcacggctg cgaagttcat ccagcatagc
     4681 gccacggata cgctacactg cgtaagttgt aaatgccgtt ccttgtaggg cgtcatagcg
     4741 ttcgacagca ttaagtaacc caatgccgcc cgcctgtagc agatcgtcaa gttccacgct
     4801 cgcgggcagt cgaacctgca ggcgcaatgc ttcgtgacgc accagcggga cataacgctg
     4861 ccacagcgag tgtttatcca ttacaccttc agcggtatag agtgaattca cgataaacag
     4921 ccctgcgtta tatgagttat cggcatgatt atccgtttct gcagggtttt taatcggacg
     4981 attagtgggt gaaatgaggg gttatttggg ggttacgcaa aaagccgaat gtaaaaaacc
     5041 ccgccggtgg cggggtttga gaggcattca gacgattaac cctgcagcag agacagaacc
     5101 tgctgcggta cctggttggc ttttgccagc acggagttac cggcctgctg gataatctgc
     5161 gctttcgaca tgttggacac ttcggtcgca tagtcggcgt cctgaatacg ggactgcgct
     5221 tcagacaggt tggtagtggt gttgttcagg ttggtgactg cggaatccaa acggttttgc
     5281 accgcaccca gggaggagcg gaatttgtcg atggagctga tggcttcatc cagagctttc
     5341 aggggatcgg ctgtcgtagc tgcttttgtt tcagcatctg tcgtcaattt accatcagca
     5401 tctttataaa ctgccttacc gctgccgtta gtcactgaac catcatcctg taggtaaaaa
     5461 tctacagtat cagatgtggt caacgcctcg ttgttatcaa cataaaccga atcaacacct
     5521 tttgttacag cgccagttgt gccgttgacg ctataacctg cagtagtgaa atcaacattg
     5581 tttcccgagg ttgatacagc tttatcgaaa gctgcctgat caacttttgc acctgttact
     5641 gaataagtaa tagtgtccgg cgtagtacta ttaccggtaa aggagataga ggtaccgcca
     5701 atgtcaatgg ttgtcgcttt ggtattattg gctgcaagtg tggacagact agcctcagtc
     5761 aaagaagcac cagagccgtt tttcgttaag cgcccagttg tatcaatgta gagtttatct
     5821 ccattgcttg ccgtaatttt accatcactg ctgatctgaa catcctgtgc agaaccatcg
     5881 atagtaatag tgcccttagc ggtatcacca actcccgggg tcaaatattt ctgaacatcg
     5941 gcagctgaag ccgttgtggt atcaaaagag tatgacttac ttgcactgtc atacttgtaa
     6001 ttcgttgcac tagccgccgt tccgaagccg ttattaatgg ttgcctgaac agtatcacca
     6061 tcactaactc cagccagcaa atcagacgct ttagcagcat cgtaacccgc actcacagta
     6121 tatttgttgc cgaccactgt tgcattagca gctaccaagt cagctttaga tgcagcagta
     6181 ttagccacag ccccgccacc attcacatta aacccattca gccccagcgt atcagagtca
     6241 attttcttca gatcaatagt gatagtctgg ccgtcattcg caccaacctg aattttcatc
     6301 gaaccgtctt ttgccagtac gttcacgccg ttgaactggg tctgaccgga tacgcggtca
     6361 atttcgtcga gacgggattt gatttcgtcc tgaatggagt ccagatccga atcagagtta
     6421 gtcccggtag aagcctgaac cgtcagctca cggatacgct gtaagttgtt gttgatttcg
     6481 gacagcgcgc cttcagtggt ctgtgcaaca gaaataccgt cgttggcgtt acgtgcagcc
     6541 tgagtcaggc ctttaatgtt agaagtaaaa cggttagcaa tcgcctgacc cgcggcgtca
     6601 tccttcgcgc tgttaatacg caagccagaa gacagacgct cgatagaact cgacagcgca
     6661 gactggttct tgttgatatt attttgagtg atcagcgaga ggctgttggt attaatgact
     6721 tgtgccatga ttcgttatcc tatattgcaa gtcgttgatt acgttttggg tttccacccg
     6781 tcggctcaat cgccgtcaac cctgttatcg tctgtcgtaa aacaaccttt agaatttttt
     6841 tcacaaacag ccattttttg ttagtcgacg aaatactctt ttctctgccc cttattcccg
     6901 ctattaaaaa aaacaattaa acgtaaactt tgcgcaattc aggccgataa ccccggtatt
     6961 cgttttacgt gtcgaaagat aaaaggaaat cgcatggcaa gtatttcatc gctgggagtc
     7021 gggtcaggtc tggatttaag ttcaatcctt gatagcctca ccgccgcgca aaaagcgacg
     7081 ctaaccccta tttcaaatca gcaatcgtcg tttaccgcta aacttagcgc ctacggtacg
     7141 ctgaaaagcg cgctgacgac tttccagacc gccaatactg cattgtctaa agccgatctt
     7201 ttttccgcca ccagcaccac cagcagcacc accgcgttca gtgccaccac cgcgggtaac
     7261 gccatcgccg ggaaatacac catcagcgtc acccatctgg cgcaggcgca aaccctgacc
     7321 acgcgcacca ccagagatga tacgaaaacg gcgatcgcca ccagcgacag caaactcacc
     7381 attcaacaag gcgacgacaa agatccgatt accattgata tcagcgcggc taactcatcg
     7441 ttaagcggga tccgtgatgc catcaacaac gcaaaagcgg gcgtaagtgc gagcatcatt
     7501 aacgtgggta acggtgaata tcgtctgtca gtcacatcaa atgacaccgg ccttgataat
     7561 gcgatgacac tctcggtcag cggtgatgat gcgctacaaa gttttatggg ctatgacgcc
     7621 agtgccagca gcaacggtat ggaggtctcg gtcgccgccc agaatgcgca gctgacggtc
     7681 aacaacgtcg ccatcgagaa cagcagcaac actatcagcg acgcgctgga aaacatcact
     7741 ctgaacctga acgatgtcac cacgggcaac cagacgctaa ccatcactca ggacacctcc
     7801 aaagtgcaaa cggcgattaa agactgggtg aatgcctata actcgctaat agataccttc
     7861 agcagcctga ccaaatacac cgccgtagat gcgggagctg atagccagag ttctagcaat
     7921 ggcgcactgc tcggcgactc cacgctgcgg acgattcaga cgcagttgaa gtcgatgctg
     7981 agtaataccg tcagttcttc cagctataaa acgctggcgc agattggtat cacgaccgat
     8041 cccagcgatg gcaaactgga actggatgcc gacaaactca ccgctgcact gaaaaaagat
     8101 gccagcggcg taggtgcatt gattgttggc gatggtaaaa aaaccggcat cacaaccacc
     8161 atcggcagca acctgaccag ttggctttcg acaacgggca ttattaaagc cgctaccgat
     8221 ggcgttagta aaaccctgaa taagttaact aaagactaca acgccgccag cgatcgcatt
     8281 gatgcgcagg tcgcgcgcta caaagaacaa tttacccaac tggacgtttt aatgacctcg
     8341 ttaaacagca ccagcagcta cttaacgcag cagttcgaaa acaacagtaa ttccaagtaa
     8401 tcattattca tcgggagaca ggtcatgtac gcggcaaaag gcacccaggc ctatgcacaa
     8461 attggcgtcg aaagcaccgt aatgagcgcc agccaacagc agttggtcac catgctattt
     8521 gatggagtgc tgagcgcact ggttagagcg cgactgttta tgcaggacaa caatcagcaa
     8581 ggcaaaggcg tctctttgtc aaaagcgatc aacatcattg agaacggact gcgggtgagt
     8641 cttgatgaag agagcaaaga cgaattaacc caaaacttaa ttgctcttta tagctatatg
     8701 gtcaggcgtt tgctgcaagc caatttacgc aacgatgtct cagcagtcga agaggtggaa
     8761 gcattaatgc gtaatattgc cgatgcctgg aaagagtctt tactctcccc ttcttcgatt
     8821 caggacccag tctgatgaac aatgcaccgc atttgtattt cgcctggcaa caactcgtcg
     8881 aaaaaagcca gctcatgtta cgcctggcaa cggaagaaca atgggacgaa ctcatcgcta
     8941 gcgaaatggc gtatgtgaat gcggtgcagg agatcgcaca cctgactgaa gaggttgccc
     9001 cgtccactac gatgcaggag cagctccgcc cgatgctgca cctgattctc gacaacgaaa
     9061 gcaaggtaaa gcagttatta cagattcgga tggatgaact ggcgaaactg gtcggtcagt
     9121 catcggtgca aaaatcggtg ttaagtgcct atggcgatca gggcggcttt gtgctggctc
     9181 cgcaggataa cctcttttga atcttaatga gccgatggct cgcgaataat ccgattacgg
     9241 ctacgcttct aatattcccc ttgaatggag tcgaagaatg cgtaatccca cgctgttaca
     9301 atgttttcac tggtattacc cggaaggcgg taagctctgg cctgaactgg ccgagcgcgc
     9361 agacggtttt aatgatattg gtatcaatat ggtctggttg ccgcccgcct ataaaggcgc
     9421 atcgggcggc tattcggtcg gctacgactc ctatgattta tttgatctag gtgagtttga
     9481 tcagaaaggc agcatcccta ctaaatatgg tgataaagca caactgctgg ccgccattga
     9541 cgccctgaaa cgtaatgaca ttgcggtgct gctggacgtg gtagtcaacc acaaaatggg
     9601 cgcggatgaa aaagaagcta ttcgcgtgca gcgtgtaaat gctgatgacc gtacgcaaat
     9661 tgacgaagaa atcattgagt gtgaaggctg gacgcgttac accttccccg ctcgtgccgg
     9721 gcaatactcg cagtttatct gggatttcaa atgttttagc ggtatcgacc atatcgaaca
     9781 ccctgatgaa gatggcattt ttaaaattgt taacgactat accggagaag gctggaacga
     9841 tcaggttgat gatgaactcg gtaatttcga ttatctgatg ggtgagaata tcgattttcg
     9901 caatcatgcc gtgacggaag agattaaata ctgggcgcgc tgggtgatgg aacaaacgca
     9961 atgcgacggc tttcgtcttg atgcggtcaa acatattccg gcatggtttt ataaagagtg
    10021 gatcgaacac gtacaggaag ttgcgccaaa gccgctgttt attgtggctg aatgctggtc
    10081 gcatgaagtc gataaactgc aaacgtatat tgatcaggtg gaaggcaaaa ccatgctgtt
    10141 tgatgcgccg ctgcagatga aattccatga agcatcgcgc atgggtcgcg actacgacat
    10201 gacgcagatc ttcactggga cgctggtgga agccgatcct tttcacgccg taaccctcgt
    10261 tgccaaccac gacacccaac cgttgcaagc cctcgaagca ccggtcgaac cgtggtttaa
    10321 accgctggcg tatgccttaa ttttgttgcg ggaaaatggc gttccttcgg tgttctatcc
    10381 ggacctctac ggtgcacatt acgaagatgt cggtggtgac gggcaaacct atccgataga
    10441 tatgccaata atcgaacagc ttgatgagtt aattctcgcc cgtcagcgtt tcgcccacgg
    10501 tgtacagaca ttatttttcg accatccgaa ctgcattgcc tttagccgca gtggcaccga
    10561 cgaatatccc ggctgcgtgg tggtcatgtc gaacggagat gatggcgaaa aaaccattca
    10621 tctgggagag aattacggca ataaaacctg gcgtgatttt ctggggaacc ggcaagagag
    10681 cgtagtgaca gacgaaaacg gcgaagcgac cttcttttgc aacggcggca gcgtcagcgt
    10741 gtgggttatc gaagaggtga tttaaatcca tccccggcgg caagccgggg agatttcatt
    10801 acggcagtgg cgtcggtaac ggcgctttat ccagcgcggc ggcacactct gccgtcgggc
    10861 gatccacacg ctccatcgtc ataccatcgt actcaatggt gttaccgtca cgctcaacct
    10921 cgtacagctc acgttttacg gtaacgttag tgagatcatc agacatcaat gtcaacttac
    10981 ccggcacggc aatcccccgc tgccactgac ggcaatccaa ggtatcgccc tctttagtga
    11041 caatcaggct gcctatcgct tccggactga ccagcgcacg ttgcggccct ttggtctgcc
    11101 agtagcccgc cagccagtcc ggcgcggggg ttttcacgac attgttatag ttttcgactt
    11161 cggcacagcc cgccagcaac atgagcgcgc ctgcaattgc gagttttttc atcattcgtc
    11221 ctgcatgaga agaaaagagg attgtggcat taaagctctg atgtcgccag cctttccagg
    11281 ttcagagaga aatttgatct aactattatt tttgtgaatt attaccccga aaataaattt
    11341 gcatggtgta tgattcgcgg ctcttgtttc aaaccttctg agttcagagg ctacattcat
    11401 gtcatggcag caattcaaac acgcctggtt gattaaattc tgggcaccca tccctgcggt
    11461 catcgcggcg ggtattctct ccacttacta ttttggcatt actggcacct tttgggctgt
    11521 cacgggtgaa tttacccgtt ggggcggtca gctcctgcag ctgtttggcg tccatgctga
    11581 agagtggggt tattttaaaa ttatccatct ggaaggatcg ccattaaccc ggatcgacgg
    11641 catgatgatc ctcggtatgt ttggcggctg ctttgctgct gcgctgtggg ccaacaatgt
    11701 caaactacgc atgccacgca gccgtatccg cattatgcag gccatcattg gcggcattat
    11761 cgccggtttt ggcgcgcgtc tggcaatggg ctgtaacctg gcggcgttct ttaccggcat
    11821 tcctcagttc tcgctgcatg cctggttctt tgccatcgcc actgccattg gttcatggtt
    11881 tggcgcgcgc tttaccctgc tgcccatctt ccgtattccc gtgaaaatgc agaaagtttc
    11941 tgccgcctca ccgctgacgc aaaaaccgga tcaggcgcgg cgtcgttttc gtctcgggat
    12001 gctggtcttt ttcggcatgc tgggctgggc gctgctcacg gcgatgaacc aaccaaaact
    12061 ggggctggca atgctgtttg gcgtcggctt tggtttactg attgaacgtg cgcaaatctg
    12121 ctttacttcg gcgttccgcg atatgtggat caccggacgt acccatatgg cgaaagcaat
    12181 cattatcggt atggcggtga gtgccatcgg gatcttcagt tacgtacagt taggcgttga
    12241 acctaaaatc atgtgggcgg gaccaaatgc ggtaattggt ggtttacttt ttggttttgg
    12301 catcgtgctg gcaggcggct gcgaaaccgg ctggatgtac cgcgcggtag aaggccaggt
    12361 gcactactgg tgggtcggtc tgggcaacgt gatcggctca acgattctgg cgtattactg
    12421 ggatgatttc gctccggcgc tggccaccga ctgggacaaa atcaacctgc tgaaaacttt
    12481 tggtccgatg ggaggcctgc tggtgacata tttgctgctg tttgctgcgc tgatgttgat
    12541 tatcggctgg gaaaaacgct tcttccgccg tgaggcaccg cagactgcta aggagatcgc
    12601 atgaaaaata tcgttcctga ttaccgcctt gatatggtgg gtgaaccctg cccttatccg
    12661 gcagtcgcaa cccttgaggc gatgccgcaa ttaaaaaaag gtgagatcct ggaagtggtg
    12721 agcgactgtc cgcagtcgat caataatatt ccactggatg cgcgtaatca cggctacacg
    12781 gtgctggata ttcagcaaga cgggccgacc attcgttatt taattcagaa gtaatccctc
    12841 attcccgctg tggcccacag cgggaacatc gttacctctg acacatagat tttacccgcc
    12901 ccctctccta ttcttttcag tatgatctca ggaggttgct atgtgtggac gctttgccca
    12961 atcccaaacg cgtgaggatt accttgcgct tctcgcggaa gatattgaac gcgatattcc
    13021 ctacgatccc gaacccattg gcagatacaa tgtcgcgccg ggaaccaaag ttcttctgct
    13081 cagtgaacgt gatgaacacc ttcatctgga tccggttttc tggggatatg ctcccggatg
    13141 gtgggataaa ccgccgctga ttaacgcccg cgtagaaact gcctcaacca gtcgcatgtt
    13201 taaaccgctc tggcaacatg gtcgggcaat ctgttttgcc gatggctggt ttgagtggaa
    13261 aaaagaaggc gacaaaaaaa gccttatttt atctatcgcg ctgacggaca acctgttttt
    13321 atggccgcga taggtagcat accgtttgag cgtggtgacg aagccgaagg atttttgatc
    13381 gtcactgcgg cggcagatca aggtctggta gatattcatg accgccgccc gctggttctg
    13441 tcgccagaag ctgcacggga atggatgcgg caagacattg gtggtaaaga agcctcagaa
    13501 atcgctacca gaagctgtgt gccagcaaac cagttcacct ggcaccctgt gtcgcgcacg
    13561 gtgggtaatg tcaaaaacca gggcgcggag ttaattcaac ctgtttgttg acatcctcca
    13621 cgccctgaat gacgaggacc cctgctacgt tcaggctgtc gcctgaatca tttcggtggg
    13681 ttcctgcttc aacgagcggc ctgactgcac catctctcca caggcaagaa cggcgtgccc
    13741 cgccgctaaa atgttacgag ctccatttac atcggcgttc gctgtatatc cacatacctg
    13801 gcatctgaat ttactttgtg acaggcgatt ttctttcgct gtatgaccac agcacgcgca
    13861 acgctggctt gtgtacgctg gcggaacagc aagcacctga ccgccacgcc agagctgctt
    13921 gtactcaagc tggcggcgca tttcat
//
LOCUS       DAZSNM010000103        13773 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZSNM010000103 DAZSNM010000000
VERSION     DAZSNM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13773
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.103"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            243..4361
                     /gene="pic"
                     /locus_tag="ACHW42_002505"
     CDS             243..4361
                     /gene="pic"
                     /locus_tag="ACHW42_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HFR8944292.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGDTVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4512..4754)
                     /locus_tag="ACHW42_002506"
                     /pseudo
     CDS             complement(4512..4754)
                     /locus_tag="ACHW42_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(4793..5743)
                     /locus_tag="ACHW42_002507"
                     /pseudo
     CDS             complement(4793..5743)
                     /locus_tag="ACHW42_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(5794..6369)
                     /locus_tag="ACHW42_002508"
                     /pseudo
     CDS             complement(5794..6369)
                     /locus_tag="ACHW42_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6373..6773
                     /locus_tag="ACHW42_002509"
                     /pseudo
     CDS             6373..6773
                     /locus_tag="ACHW42_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7176..7757)
                     /locus_tag="ACHW42_002510"
     CDS             complement(7176..7757)
                     /locus_tag="ACHW42_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944293.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(7804..11661)
                     /gene="sigA"
                     /locus_tag="ACHW42_002511"
     CDS             complement(7804..11661)
                     /gene="sigA"
                     /locus_tag="ACHW42_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR8944294.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(12022..13026)
                     /locus_tag="ACHW42_002512"
                     /pseudo
     CDS             complement(12022..13026)
                     /locus_tag="ACHW42_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            13011..13388
                     /locus_tag="ACHW42_002513"
                     /pseudo
     CDS             13011..13388
                     /locus_tag="ACHW42_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13427..>13773)
                     /locus_tag="ACHW42_002514"
     CDS             complement(13427..>13773)
                     /locus_tag="ACHW42_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8944295.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         3448 a         3239 c         3359 g         3727 t
ORIGIN      
        1 aggctgggcc atatcccacc ggcagaagca gaaaaagctt attatgcttc catcggaaat
       61 gatgatctgg cagcctgagt tcacagataa aacactctcc aggaaacccg gggcggttca
      121 gttcacaaaa acacattaat gcagtaacta tattttcctt tctggtgata acgtcgggtt
      181 atcattagct tcttcagcta ttttactttt atatcccttg taaacatcat ggagaatcca
      241 tagtgaataa agtttattct cttaaatatt gccccgtcac cggggggctt attgctgtct
      301 ctgaacttgc ccgcagggta ataaaaaaga catgccgaag attaacgcat attcttctgg
      361 ctggcattcc agcaatctgt ctgtgttact ctcagatatc ccaggcgggt attgtccgtt
      421 ccgatattgc ctatcagatt tatcgtgatt tcgccgaaaa caaagggctt tttgtacctg
      481 gtgccaatga tattccggta tatgataagg acggaaaact tgtgggaaga ctgggtaaag
      541 ccccaatggc cgatttcagc agtgtgagct caaatggcgt tgctacgctt gtatcgcctc
      601 agtatatcgt cagcgtaaag cataacggag gatatcggag tgtgagcttt ggtaatggga
      661 aaaatacata ttcccttgtt gaccgtaata accacccttc tattgacttc catgctccac
      721 gtctgaataa actggttaca gaagttattc cctcagcggt aacatcagaa ggaaccaaag
      781 ccaatgctta taaatacact gaacgttaca ccgcttttta tcgggtgggt agtggtacgc
      841 agtacactaa ggacaaggac ggaaatttag ttaaggttgc cggtggatat gcttttaaaa
      901 caggaggaac cacaggagtt cctctgatat ctgatgcaac aatagtctct aatcccgggc
      961 aaacttataa tcctgtaaac ggccctttac ctgactatgg agcccctggg gatagtggtt
     1021 ctcctttgtt tgcttatgat aaacaacaaa aaaaatgggt tattgttgct gtattaagag
     1081 catatgcagg tattaatggt gctacgaact ggtggaatgt cataccaaca gattatctga
     1141 accaggttat gcaggacgat ttcgatgccc ccgtagactt tgtttccgga ctgggccccc
     1201 tgaactggac atacgacaaa acatcaggca caggtaccct gagccagggc agtaaaaact
     1261 ggaccatgca cgggcagaaa gacaatgacc tcaatgccgg taaaaatctg gtattcagcg
     1321 ggcagaatgg tgcaattatc ctgaaagaca gtgtgactca gggtgccggt tatctcgaat
     1381 ttaaagacag ttacaccgta tctgctgaat ccggaaaaac atggacgggt gccggcatta
     1441 ttactgacaa ggggacgaat gtaacctgga aggtcaacgg cgttgccggt gacaacttgc
     1501 ataagctggg ggaaggaacc ctgaccataa acggaacagg tgtaaacccg ggaggactga
     1561 aaacgggaga cggtatcgtt gtacttaacc agcaggcaga cactgcaggt aatatccagg
     1621 ccttcagttc agtgaacctc gccagcggac gtccgaccgt ggtgctcggg gatgcccgtc
     1681 aggtcaatcc ggataacatt tcatggggat accggggagg taagcttgac cttaatggta
     1741 atgccgttac cttcacccga ctgcaggctg ctgattacgg ggcggtgatt acaaataatg
     1801 cacagcaaaa atcccagctt ttactggatc ttaaggctca ggatacaaat gtcagtgaac
     1861 cgacgattgg aaatatatcc ccctttggtg gtaccggcac tccaggaaac ctgtacagca
     1921 tgatactcaa cagccagacc cgcttctata ttctgaaatc tgccagctat ggtaacactc
     1981 tgtgggggaa cagcctgaat gatccggctc agtgggagtt tgttggcatg aacaaaaaca
     2041 aagcagttca gacagtaaaa gataggatcc tggccgggcg ggcaaaacaa cccgttatct
     2101 ttcatggtca gctgaccggg aatatggatg tcgccattcc acaggtgccg gggggaagaa
     2161 aggtaatctt tgatggtagc gtgaacctgc cggaaggtac cctgagtcag gacagtggca
     2221 ccctgatatt ccagggacat ccggttatcc atgcctccat cagtggcagt gcaccggtca
     2281 gcctgaacca gaaagactgg gaaaaccgtc agtttacaat gaaaacactg tcgctgaaag
     2341 acgctgactt ccatctttca cgtaacgcct cgctgaacag tgacattaag tcggataaca
     2401 gccatatcac actgggaagt gacagggcat ttgtggataa aaatgacgga acaggaaatt
     2461 atgtcattcc ggaggaaggt acctctgtcc cggacaccgt gaatgacagg agccagtatg
     2521 aagggaatat tacgctgaac cataactcag ccctggatat cggcagcagg ttcaccgggg
     2581 ggattgacgc ttatgacagt gccgtcagca tcacctctcc ggacgtcctg ttgacagccc
     2641 cgggtgcttt tgccggcagt tcactgacag tgcatgatgg cggtcatctt acagcactga
     2701 acggtctttt cagcgacggg catattcagg ccggtaagaa cggcaaaatc accctgagcg
     2761 gtacaccggt taaagatacg gctaatcagt atgcccctgc tgtatatctg acggacggat
     2821 atgacctgac cggcgataac gcagcactgg aaattacccg tggagcacat gcttccggtg
     2881 atattcatgc ctctgcggca tcaacagtta ccatcgggtc tgacacgccg gcagaactgg
     2941 cttctgcgga aacggctgca tcggcgtttg ccggcagtct tcttgagggc tataacgcag
     3001 cattcaatgg tgccataacc ggtggcaggg ctgatgtcag tatgcataat gcactgtgga
     3061 ctctgggtgg ggactctgcc atccacagtc ttaccgtcag aaacagccgt attagttctg
     3121 aaggagaccg tacattccgt accctgacgg tgaataaact ggatgcaaca ggcagtgatt
     3181 ttgttttgcg tacggacctg aaaaatgccg ataaaattaa tgtgactgaa aaagccactg
     3241 gttcagataa cagcctgaac gtcagcttta tgaataatcc tgctcaggga caggccctga
     3301 atattcctct ggtcacggca ccggcgggaa cttcagcaga gatgtttaag gccggcaccc
     3361 gggtgacagg tttcagtcgg gtgaccccaa ccctgcatgt tgataccagt ggtggcaata
     3421 cgaagtggat actggatggt tttaaagcgg aggctgataa agccgctgcc gcgaaggctg
     3481 acagtttcat gaatgccggg tataaaaact tcatgacgga agttaacaat ctgaacaaac
     3541 gtatgggtga cctgcgtgac acaaacggtg atgccggtgc ctgggcgcgc atcatgagtg
     3601 gtgccggttc tgcagacggt ggttacagtg ataattacac ccatgttcag gtcggctttg
     3661 acaaaaaaca tgaactggac ggtgtggacc tgtttaccgg tgtcacgatg acctataccg
     3721 acagcagtgc agacagccat gcattcagcg gaaagacgaa atcggtgggg ggcggtctgt
     3781 atgcttcagc attgtttgag tccggtgcct atatcgattt gattggtaaa tatattcacc
     3841 atgacaatga ttacacaggt aactttgcta gcctgggaac gaaacactac aacacccatt
     3901 cctggtatgc cggtgctgaa acgggttacc gctatcacct gacagaggac acgttcattg
     3961 agccgcaggc tgaactggtt tacggcgccg tgtccgggaa aacattccgc tggaaagacg
     4021 gtgatatgga cctgagcatg aagaacaggg acttcagtcc gctggttgga agaacagggg
     4081 ttgaactggg caagaccttc agtggtaagg actggagtgt gacggcccgt gccggaacca
     4141 gctggcagtt tgacctgctg aataatggag ataccgtact gcgtgatgcg tccggggaga
     4201 aacggataaa aggagagaag gacagccgga tgctgtttaa tgttggtatg aatgcgcaga
     4261 taaaggacaa tatgcgcttt ggtctggagt ttgagaagtc agcctttggt aaatataacg
     4321 tggataatgc ggtaaacgcg aatttccggt atatgttctg atgtgggggt aaagggctgc
     4381 cggaaggcag cctttctcag agactcttaa aatataaaac tgaaggtctt cggtaagggg
     4441 gagtgtatta gacgtcaact agttttgccg actggcgcga gcctgtttaa gattctgtgt
     4501 aaatgccttt ctcagaagtg accgtccagg cggtcaccga actcgataat aaagcggctc
     4561 attgccattc gccagtcctt caacggcatc gtccatttct gggacgcaga ctggattgcc
     4621 agccacacca cttttttcac cgagtcgtct gtcgggaaca ctttacgctt tttgatcgca
     4681 tggcggatca cgctgtttag cgactcgatg gcattcgtcg tatagatcac tttgcggatg
     4741 tccgttggat aagcggtaag cgtacagcga gggccgtatt gacggggatg tgttattcag
     4801 ctggcagtgc tatgcgccac ggaagcagtt cgctgacccg gttgaccggc cagtctgcta
     4861 tgacgccaag cacatggcga aggtagcttt ctggatccac gtcattcagt ttgcacgtcc
     4921 cgatcaggct gtacagtagc gcgccccgct caccaccatg gtcagagccg aagaacagga
     4981 agtttttacg acccagactg accgcccgca gggcattttc agcgatgttg ttgtcgattt
     5041 ccacccagcc attactgcag tacacgttca gggccttcca ctgtttcagt aggtatgcga
     5101 acgcttttgc cgtatccgag tgacgcgaca gtgttttcat ctgttgctgt atccggtcat
     5161 acagtgactg catcagtggc gcggctcttg cttttcttgc cgccagacgc tgttctgctg
     5221 aacatccccg gacttctgcc tcgatggtat acagttcacc gatacgctgc agggcttccg
     5281 tggtgatgtc ggttggcact cttgcatgca catcgtggat ttttctccgg gcatgggcca
     5341 tacacgcagc ttccgttatt ctgccggatt cgtataacgc ccggtaacca ccgtaagcat
     5401 ctgcctgaag cacaccgctg taaccggcta ggtgattctg tggatggata cctttccggt
     5461 ccgggctgta cgcgaaccag accgccgggg gcatctgtga accggcgtta cggtcatcac
     5521 ggacatagac ccacagccgg gcagttcttg ttttaccact gcccggctcc tggaccggaa
     5581 cggggatatc atccgcatga actttaccgg gcatcagtac atactggcgc aggacgtcat
     5641 acagcggctc cagcagttca gcaacggcac cggtccagcg acccagtgtg gcacggctca
     5701 gctccacgcc ctgacgacgg tatatttctg actggcggta taactgtcaa cgacggatga
     5761 aaagtggctc ttccgcaacc tgggtggaga ttttcatgcc agtggactca ttaccctctg
     5821 cctgagtagt tcaaatcctg ctcgaccata catctggcgt ttcagcatct tcttcaggcg
     5881 atttacatga ccttcgacaa caccattact ccagcgacta ctgattgcct cacatatagc
     5941 tgcagcatca gcttccatcc ccgccgccac gcgccgaaat tctgctgaac cgctttcgtg
     6001 gactcgagtg aaccaactgc ttagctgtga tttattttgg gttttcagaa tacggtagaa
     6061 ctcgagtgcc agttgctgcg ctattttcag ctcaggttct ttttcacaca tcagactaat
     6121 aaatcgggaa gcataatttt cttcatccct ggttattctc cagggcatca accagcggct
     6181 tacccgggag actgaaggaa gtctgacggc agtagtaacc ggtgggttcc agcctttacg
     6241 ccatttggca accgcatccc tgacgatggt ttcactgcct gtaaatcccc gggccaccat
     6301 ttcccgccat atccggctgg cgttataatt accactttct cgttgctctt ttaaccactc
     6361 cctccatggt gtcagcgaaa aacgaaaccg ctgttgaaat ccctggaaag ctggttgcgg
     6421 aaaagatgaa gacccagtcg cgacactcag agttggcgaa ggcgttcgcg tacgcactta
     6481 accagtggcc ggcactgacg tactatgcga acgatggctg ggtggaaatc gacaacaaca
     6541 tcgctgaaaa tgccttgcgg gcggtcagtc tgggtcgtaa aaacttcctg ttcttcggct
     6601 ctgaccatgg tggtgagcgg ggagcgctac tgtacagcct gatcgggacg tgcaaactga
     6661 atgacgtgga tccagaaagc taccttcgcc atgtgcttgg cgtcatagca gactggccgg
     6721 tcaaccgggt cagcgaactg cttccgtggc gcatagcact gccagctgaa taacacatcc
     6781 ccgtcaatac ggccctcgct gtacgcttac tcatcacctg ctattaatcc ccgagtcagt
     6841 acttcgcggc ctgacgcgat caccctactc agtacttcag agcccgcctc cggtacctgc
     6901 ctggcgatcg cccctgtgca acaaaataaa tcaaccgtta tcaaaccacg atcgttcgta
     6961 tgcgccgccg atgacgcgcg cctgaaaaac gcgtcaatat gagttccccg gaagcttacg
     7021 ataatacgct tatgaacatt acaacacgct catgcggaac ctggatgggg gaaggaacgg
     7081 tgaagctgtt agcgaatgac cggcgtttat acaaacgccg gtcatgatga atgatatttc
     7141 agaatatacc tcgggctaaa ataccgggac agagatcagg cgtgattaat ctgcagcatt
     7201 tttaataaca gaagtactga gcttacttta cctttgatga tgtaatagtt atttccgtcg
     7261 gtactcattt ttccctgaaa taatgtcacg gatgtttctt cctgctgatc ctgcaggcgc
     7321 agtcgtaagg tcatattttt aatgagctga gatccgggtg gcaaggggta tttgctgtca
     7381 gatactgggc gactgagtct gatgtggcgc gtatacagcc cgttttcgta cagggtggcc
     7441 tctgttatgg tgtcagtgac cagcatgtgg gagacaatgc tgtattcttt tttaaagcac
     7501 ccaccagaaa cggtgattgt aatgataatg gtcttctcga tcgagatgta aatacccgtt
     7561 tcgttatact gtccgtatcg gaaccatgag aactcagttg cattccagcc atgctccaga
     7621 atatgtgcat ttttttgcct gatagtttct ttttttattc gctcatgttt aaggcataac
     7681 aggcagataa ataatgtcag aaaaagaaag ctaataatga gagcaagagc gatatagtct
     7741 gtcacagggt aatacatatt ctctccggta gaagaagggc cgcaaacgcg gcccgggctg
     7801 ttatcagaaa gagtaacgga agttggcgtt gatcgcgtta tccacgttgt atttaccaaa
     7861 tgccgatttc tcaaactcaa gaccgaagcg aagattatcg cgaatttcgg cgttgagacc
     7921 aacattcatg agaatacgac cgtctttttc acctttgata cgtttctcac cggacgcatc
     7981 acgcagtacg gtttcaccgt tggcaaacag gtcaaactgg tagccaaggc cggcgcgggc
     8041 tgtgactttc cagtccttac cggagaagga tttacccaca tcaacaccgg tacgcccaat
     8101 cagcggatta aagtccttat ccttcatggt cacgctcatt ccgcgatcct gccagtcgaa
     8161 ccgtttacct gatacggccc cataaaccag ttctgcctgc ggctcaatcc aggagtcttc
     8221 tgttacatga tagcggtagc ctgcttcagc acccacatac aaggaatgag aactgtagtc
     8281 ttttgttccg agtccagcaa aggtcgccga gtactcatta tcatggtgaa catacttact
     8341 aatcaggtcg atataggcac cagagtcaaa tatagcggaa gcatacagac ctgccccgac
     8401 ggacttcgtc ttgccactga atgcattact gctggcatga ctgtcggtat acgtcatagt
     8461 cagaccagtg aaaaggtcaa gtccatccag ctcatgtttt ttatccacac caatctgcac
     8521 atgggtgtag ttgtcactgt atccaccaga agctgaacct gctccgctca tgatgcgggc
     8581 ccatgcaccg gcctcgccat tggtgtcacg cagatccccc attcgtttat tcaggttgtt
     8641 gacttctgtc aggaaggctt tgtaattacc ggacatatac gattttgctg caccggaaga
     8701 tgctgtattt tcagccacct tatatccggt cagggtccac gtggtatgtt cctgatcctc
     8761 tttcgtgctg atcacaggag atatgttgct gaaacctatg gcctgagtac tggctttaaa
     8821 tatctcttta ttgcttcctt ttggggctgt tatcaggtca acgtttaatc ctgagttatt
     8881 atttcccact ttctgtatat cgaccgacag actgttattg cttccggtta actgattttt
     8941 aacaatgagt ttatccgatt gatttacaga ctggctcaga tcagcccgca taatcacagc
     9001 agtattagtg gcatccagtg tgtttactgt cacagtatgg aaacccgaat tctctatgtt
     9061 actaaacaac aaaagactgt cgcgggtttt taatgattgc agggctgact gtccgtttac
     9121 tctccataag gtgttataca tgctggctga ggctgcagga gcatcaatac ctccggtata
     9181 agaagtatca aagccaccca gcaaacctgt agccagcggt gtgtgtgtgg ttttgtccag
     9241 gttatcttct ttatactggt caacaccaaa cagaatagat gcttttttgt ctgattttat
     9301 attaccattg acccaggcat aattttgagc ctttaacgtg gcaccatctt ctgtcatatt
     9361 atatccatct gacatataga ttgccgggct gtatttatta ttttctgtag gggtgcctgt
     9421 taatgtcagg gttccggcca gttcaatagc tttatctaca tacatttttc cctgggcggt
     9481 aacagtagct cctttgttga taatcaggtc tgtattaatt aaggataatg caccagtcat
     9541 agtaacagtt gatttatcac caacagttat tttgctgttt tgagcactca gactgccgtt
     9601 gaaactactt tcgcctgctg aatccccgga gcgaagctgt tgtctgaatc caaaaccttc
     9661 gccagtaatg tttcttcctg agaacatgtc aatgtatgca gttttatttc ccagaacaat
     9721 ttgagagttt ttagcctcta tgtgtccttt tacattagca tcccggccta tactcagggt
     9781 tgcgttttct aaatttaatt tccggaaatt aaatttacga ctttcccagt cgggctggga
     9841 aaaagaggct atctgattgt tggacttata ttccgtattg taacgtttat ttacagtgct
     9901 ttcctggtcc tttatggcag cagaatagtc tttttgacat aaaaaaggaa ttggacagtt
     9961 gttattgcct tctttaaata tggcatgttc tgtcgcatgt ccttgtaatg ttaactgtgc
    10021 attccggaca gagatatcgt ttttgatatc gactgagcca tcaaagatca gattggcatt
    10081 attgttatgt tgctgggtag tattgataat atgatttatg cttatattac ctgatacatt
    10141 cccatggtac atatatgcat cagtattagt aagtgataat gttgattgct tcacgtttga
    10201 gttcgttacg gttgttcccg catctgttgc tgcgattttc tgaaatgact ggtcatagcc
    10261 gtttagatcc agtgttccgc catttctggt aaaatagatt tcgccattac cgctttcgct
    10321 taaagcgtct ttggcattta tttttaccgt acctttacct ccggccatgt aaattttatt
    10381 gaaggctttt tcagcactaa ggatgacagt cccattacct attttaaggt tatttccctg
    10441 tgctattttt acatccagag taccactacc tattttatgc agattatctc cggcaacgcc
    10501 cttaacattc cattcaacgg tggtattatt atcaatatct atcccggcac ctttataaga
    10561 cttatctttt ccgtacactt tatatgtctg gttcttatcg aatataagcc cgccaatgcc
    10621 aagatcggta tcctgcttga gcgttaatac ccctcctcct gagacagaca ggtccttatc
    10681 ttttataatc gatgataact cttgtttttt accatccagc tgcatagtgt ttgtgaaatt
    10741 cttttttaaa ttgtttacca gaatattatt aaataacgag tagtttgtcc aggctatatt
    10801 ggtactactg ataaaaccaa ctgtactggt aacaccatgg aaaacccatt tttgttgttc
    10861 tttatcatat agataagaac cagaaccact atcgccaaaa attggtgagt ttttaaaagg
    10921 ggttctgttg gtataaatgt aactcttgtt tttccagtcc agattaaaaa gacttgcgct
    10981 taacaagtcg ggattatagt tttgtccaaa cagatattgt ttgccgttta atatcgttga
    11041 ggttgttccg gcacctgctc tgaagccaat tatttgttcc ttaccgttgt agtttacgcc
    11101 atatcttttc atcgcatctt cgggagaaag tgagaagtcg gcactctccg taacccctgt
    11161 tgtttccacg acgaatttat tcagacgatg aaccgaaaag tcgccagatg atactctgtt
    11221 acttgcttta tatgctgtct gatcccatga ctgtgttgtt attgcatgat gctgtgtacc
    11281 gttatgagtc gcagtaacac tgtacgcacc tccaattgat gttgttgcac ctttgttgga
    11341 aacagcagaa aaatcaggta caggtagttc tggaaaatga aatatctttc catctttcat
    11401 cattaattga acatttgttg cacccggtct gaactctcct ctgttttgag caaggtcaag
    11461 ataatcgcgg gtccatatat tatttatatc cagcattgct gcaaatgctg tttcgccata
    11521 acttccatat attgaggata aaataatacc gaggataact ttctttcttg atgtaccgac
    11581 actaactttc cgggtcagtt cagaaacagc aactaatcca cctgtaatat gactatattt
    11641 cagtgaataa attttattca tacgtgaact ccgatatttc tgagttgtgg ttatgggata
    11701 gcatttaatt acaacgggga actatcagtc atggtacgta actgataagg gtataatgat
    11761 aatcactgtc agttatattt gtatgacaga tgtaacaaaa tgaaaaaaaa gataagcaca
    11821 agtgattgat tggttagata ttgatcactt ggtgtttgta ttttgaattt tgcgatcaaa
    11881 atttgatctt tgttgtttcc ggtcaatcct ttacaggttt tctgtattat ccgggggagt
    11941 taagtctgtc gatgtccgac agacttttga tgatggaaac aacatgcagc gataatgtgc
    12001 atgctgtttt gcaggtggaa ctcagaaggt gaactggaac tgtgcgaccg caccgtaagt
    12061 gttgtccgtg ccgatcaggc ctgcaacatc aaggtgaacc acatcaaacg gtgagatccc
    12121 caccccggcc gtgaatgcac tgccgtcgtt accggccaga ttctgacggt aaccggcgcg
    12181 cagctgtgcc catttccagg cattaaattc tgtgccaatg gcggcaaact gacggttgct
    12241 gtcggaggtg aaaccgctgg ccggggtcag atccacatca aatgcagtgg tgaacatcgc
    12301 gttgtgccag gagacaccgg ccgtcacctg cgggcggatc ctgaaggttt ccgtgatacc
    12361 gttcacttct tttgtctcaa tactacggga gatcaggttc tgtgcgacca gtcccagcgt
    12421 ccagttgtca tccaggtccg tactggcacc gatgtcggcg ttgatgccat ttttcgtgtt
    12481 gtggtagcgg tcgcctttga aggcgctgct gtcataattt ctgaccagca cgttgtagtt
    12541 gaacaggtcg acgcgctgga atttgggggg gaagcccaga gaccatttct gcccggccgt
    12601 ttccagttcc gtagccagag caatacccac atcagtgatc agtgccgtgc gggcacaggc
    12661 ctgtgatgtc aggcggcttt tgtccacctc ctgcccggtg ctgtttgcca cctcttccag
    12721 gtaagtgagg tcagcatcag agactttacc gtcgacactg acggtgccgt gtgatttaac
    12781 catcagtgcg gcagagaggc gatcaccggg aagggccgcc acagcagaaa cgcccagctg
    12841 tgcggcggca tgtgtgttac ggaagtcccg gaggtgttct ttcaggcgtg cggcagcctc
    12901 aggaacaccg tggttacttt cgatggcgcg gtcgaaggcc ttccactcat cactgatccg
    12961 gtcggcgttg tcggtgatat tatccggatt ggagagctgc gcgccgacgg acggaagcag
    13021 gatgctgggc tcatgcgcga cgcaaaatcc acgatgtata tatcagtact aaaagcgcga
    13081 cggcggaaga agctctgaaa ctaatcggtg aactgtacgc catagagcac gatatacgcg
    13141 ggttaccggt gtctgaacgc ctggcggtca ggcaaatgca gagtagaccg ctcctgactt
    13201 ccctgtataa gctgatgcag gagaaagaac acacgttatc gaagaaatgt cgtctgggag
    13261 atgcgttccg gtatatcagg aagcactggg ctgcgttgtg caacttctgt gatgatggcc
    13321 tggctgaggt agataataat gcagcagaaa gagcgcttcg tgcagtctgt ctcgggaaga
    13381 aaactttttc gaggatcact cattccgtgt ctgactgctt cagggtttaa ttttgcttct
    13441 gctgactgaa tgatgattgg gcagcaagtg caatatccca cataaatcca gtcaactcac
    13501 gagccaccgc cgtaatggct acttgtgatt ttttcccttt tgctgtcaga gttcggtaac
    13561 gagaacacaa tcgttgttga gctttccatg caatatcttt ggaatattgt gttacagagt
    13621 ccggtcggta atatataagc catgctccaa cttttgcagg tgtacgataa gaccaggcgg
    13681 cttcgtaaag cagacgtctc agttcgctgt ttccaacttt tgttattcct ctgggacgaa
    13741 tactgtttcc actggaatat tcacctggca cca
//
LOCUS       DAZSNM010000104        13723 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZSNM010000104 DAZSNM010000000
VERSION     DAZSNM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13723
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.104"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="ACHW42_002515"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="ACHW42_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR8944296.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /locus_tag="ACHW42_002516"
     CDS             321..1952
                     /locus_tag="ACHW42_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR8944297.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="ACHW42_002517"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="ACHW42_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR8944298.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="ACHW42_002518"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="ACHW42_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR8944299.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="ACHW42_002519"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="ACHW42_002519"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR8944300.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="ACHW42_002520"
     CDS             6426..7055
                     /locus_tag="ACHW42_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR8944301.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="ACHW42_002521"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="ACHW42_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR8944302.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10059)
                     /gene="melA"
                     /locus_tag="ACHW42_002522"
                     /pseudo
     CDS             complement(8718..10059)
                     /gene="melA"
                     /locus_tag="ACHW42_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
     gene            10342..11250
                     /gene="melR"
                     /locus_tag="ACHW42_002523"
     CDS             10342..11250
                     /gene="melR"
                     /locus_tag="ACHW42_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR8944303.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11449..13716
                     /gene="adiA"
                     /locus_tag="ACHW42_002524"
     CDS             11449..13716
                     /gene="adiA"
                     /locus_tag="ACHW42_002524"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR8944304.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         3581 a         3559 c         3335 g         3248 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg agttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcca ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tacgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaacaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aatcgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcgc ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac tggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataactg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcttggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgatg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg ggttctcgca
     9661 aatttgccac agatgcggaa tggtacgtag cgcgcgcata ataccgcccg gccccaacgt
     9721 atcggcaatg gtttgttcca gaccatgccg cttacagacc tcgaaatcag tcaccgtgca
     9781 aggttcataa ccgccaatct gaaatgccac cacgacaaaa tcggcatcct ctaaggcttc
     9841 tttctgttgg gtgtggcagg tgattttgcc gctggcccct gctgaatcca tcagcttacg
     9901 caccacaata tgcgactctt ccaggcgggt ggggtcaatg tccatcaggg caatatgcgc
     9961 cgttttcagc gcctcgcgat ggaacacatc accaagaata tttttaacga aaatcgtcga
    10021 accagcgccg ataaatgtaa ttttgggtgc agacatcatg cagatctcct ggcttgcttg
    10081 aataacttca tcatggcagg cgaatctgca gaaaacctcc ggcttccagg ttgagcattc
    10141 ccataaactc agatcctgcg tgaagcagca gtaaatctga gtttatggga ataaattacc
    10201 tgaaaagtag agttttcggc ctcatgatgc tcggacagtc ccctcgcccc tctgggaaga
    10261 gggtgaaaac cgtgctccca ctcgcagtca tcctccctca ctcctgccat aattctgata
    10321 ttctaggaaa gagagccatc catgaataca gatacgttta tgtgcagcag cgacgaaaaa
    10381 cagacccgca gcccgctgtc gctgtattcg gaataccagc ggatggagat tgagtttcgc
    10441 gcaccgcata tcatgcccac cagccactgg catggtcagg tcgaagtgaa tgtgcctttc
    10501 gatggcgatg tggaatacct gatcaacaat gaaaaagtga atatcaatca gggtcatatc
    10561 acgctgttct gggcctgtac accgcaccaa ctaacagata ccggaacctg tcagagcatg
    10621 gcgattttta atctgccgat gcatctgttt ctctcctggc cgctggataa agacctgatt
    10681 aaccacgtca ctcacggcat ggtgatcaaa tcacttgcca cacagcaact tagcccgttt
    10741 gaagtacgcc gctggcagca ggaattaaac agtccgaatg agcaaattcg ccagctcgcc
    10801 attgatgaaa ttggcctgat gctcaagcga tttagcctct ctagctggga accgattctg
    10861 gtcaataaaa cctcgcgcac acacaaaaac agcgtctcgc gccatgcgca attttatgtt
    10921 agccagatgc tgggctttat tgccgaaaac tatgatcagg cgctgaccat caacgatgtg
    10981 gctgagcacg tcaaacttaa cgccaactat gcaatgggga tattccagcg ggtcatgcaa
    11041 ttgacgatga aacagtacat taccgcgatg cgcatcaacc acgttcgcgc gttactgagc
    11101 gataccgata aaagtattct cgatattgcc ctgacggcag gctttcgttc aagtagtcgt
    11161 ttttacagca cgttcggcaa atatgtcggc atgtcgccgc aacaataccg caaacttagc
    11221 caacagcgcc gccagacgtt tcccggctaa agacgatatc agtatcagcc aaaaaaatag
    11281 tttagccgtc gataataagc agtgaaaata atttaaaata atcacataac gtattgttta
    11341 taaaactaaa tatttcaaaa atgtttgttt ttcacgcgct ttacagcccg aaaaggccgg
    11401 aagatacttg cccgcaacga agattccttc ataaccgggt aagcaatgat gaaagtatta
    11461 attgttgaaa gcgagtttct ccatcaagac acctgggtcg gtaacgccgt tgagcgtctg
    11521 gcagatgctt taagccagca aaatgttacc gtgattaaat ccacctcctt tgatgatggt
    11581 tttgccattc tctcttcaaa cgaagccatt gactgcctga tgttcagcta tcaaatggag
    11641 catccggacg aacatcaaaa cgtcagacaa ttgatcggta agcttcatga gcgccaacaa
    11701 aacgtgccgg tcttcctgtt gggcgatcgg gaaaaagccc tcgccgcaat ggaccgggat
    11761 ctgctggagc ttgtcgatga attcgcctgg attctggaag ataccgccga ctttatcgcc
    11821 ggacgcgccg ttgccgcgat gacccgctac cgccagcagc tgttgccgcc actgttcagc
    11881 gcgctgatga aatatagtga catccatgaa tattcctggg cagcgccagg ccaccagggc
    11941 ggcgttggtt ttaccaaaac acccgccgga cgtttctacc atgactacta tggtgaaaat
    12001 ctgttccgta ccgacatggg catcgaacga acttccctcg gttctttgct tgaccatact
    12061 ggcgcatttg gcgaaagcga aaaatatgcc gcacgcgtat ttggtgccga tcgctcctgg
    12121 tcggtagtcg tcggtacttc cggctctaac cgcaccatca tgcaggcttg catgaccgat
    12181 aacgatgtcg tggtcgttga ccgtaactgc cataaatcca tcgaacaagg tttgatgctg
    12241 acaggcgcga aaccggtcta tatggtgcca agccgcaacc gctacggcat tatcgggcca
    12301 atctatccgc aggaaatgca acctgaaacc ttgcaaaaga aaatcagtga aagcccgctg
    12361 accaaagaca aagccgggca aaaaccgtct tactgcgtgg taaccaactg cacctatgac
    12421 ggcgtgtgtt ataacgctaa agaagcgcag gatctgctgg aaaaaacctc cgatcgtctg
    12481 cactttgacg aagcctggta cggctatgca cgtttcaacc cgatctatgc cgatcactat
    12541 gccatgcgcg gcgaacctgg cgatcacaac ggtcctaccg ttttcgccac ccactccatc
    12601 cacaaactgc tgaatgcgct gtcacaggct tcttatattc atgtacgtga aggtcgtggg
    12661 gcgattaact tctcccgctt caaccaggcc tacatgatgc atgccaccac ctccccgctg
    12721 tatgccatct gcgcatccaa cgacgtggcg gtgtcgatga tggacggcaa cagcggcctg
    12781 tcactgacac aggaagtgat tgacgaagcg gttgatttcc gtcaggcgat ggcgcggcta
    12841 tataaagagt tcaccgctga cggtagctgg ttcttcaaac cgtggaacaa agaagtcgtc
    12901 accgacccac aaaccggcaa aacctatgac tttgctgacg caccaaccaa actgctgacc
    12961 accgttcagg actgctgggt aatgcatccg ggcgaaagct ggcacggctt caaagatatt
    13021 ccggataact ggagtatgct cgacccaatt aaagtcagca tccttgctcc gggaatgggt
    13081 gaagatggtg aactggaaga aaccggtgtt ccggcggcgc tggtcactgc ctggcttggt
    13141 cgccacggca ttgtgcctac ccgcaccact gacttccaaa ttatgttcct gttctctatg
    13201 ggcgtaaccc gtgggaaatg ggggactctg gttaacaccc tttgctcctt caaacaccac
    13261 tatgacgcca acacaccgct ggcgcaggtg atgccggaac ttgttgaaca atatcctgac
    13321 acttacgcga acatggggat tcacgatctg ggtgacacca tgtttgcctg gctgaaagaa
    13381 aacaaccctg gcgcacggtt gaacgaagcc tattccggcc tgccggtggc ggaaatcacc
    13441 ccgcgtgaag cgtacaacgc gattgtcgac aacaatgtcg aactggtatc cattgaaaat
    13501 ctgccaggac gcatcgcggc aaactcagtt atcccgtatc cgccaggaat cccgatgctg
    13561 ctgtctggtg aaaacttcgg cgataaaaac agtccgcaag taagttattt acgctcgctg
    13621 caatcctggg accaccattt ccctagattt gaacacgaaa ctgaagggac tgaaattatt
    13681 gacggtattt accacgttat gtgcgtgaaa gcgtaaccac tat
//
LOCUS       DAZSNM010000105        13535 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZSNM010000105 DAZSNM010000000
VERSION     DAZSNM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13535
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.105"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            47..574
                     /gene="gntK"
                     /locus_tag="ACHW42_002525"
     CDS             47..574
                     /gene="gntK"
                     /locus_tag="ACHW42_002525"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR8944305.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            578..1918
                     /gene="gntU"
                     /locus_tag="ACHW42_002526"
     CDS             578..1918
                     /gene="gntU"
                     /locus_tag="ACHW42_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR8944306.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(1975..2568)
                     /gene="yhgN"
                     /locus_tag="ACHW42_002527"
     CDS             complement(1975..2568)
                     /gene="yhgN"
                     /locus_tag="ACHW42_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR8944307.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2760..3863
                     /gene="asd"
                     /locus_tag="ACHW42_002528"
     CDS             2760..3863
                     /gene="asd"
                     /locus_tag="ACHW42_002528"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR8944308.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            4136..6322
                     /gene="glgB"
                     /locus_tag="ACHW42_002529"
     CDS             4136..6322
                     /gene="glgB"
                     /locus_tag="ACHW42_002529"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR8944309.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            6319..8292
                     /gene="glgX"
                     /locus_tag="ACHW42_002530"
     CDS             6319..8292
                     /gene="glgX"
                     /locus_tag="ACHW42_002530"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR8944310.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            8310..9605
                     /gene="glgC"
                     /locus_tag="ACHW42_002531"
     CDS             8310..9605
                     /gene="glgC"
                     /locus_tag="ACHW42_002531"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR8944311.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9605..11038
                     /gene="glgA"
                     /locus_tag="ACHW42_002532"
     CDS             9605..11038
                     /gene="glgA"
                     /locus_tag="ACHW42_002532"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR8944312.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            11057..13504
                     /gene="glgP"
                     /locus_tag="ACHW42_002533"
     CDS             11057..13504
                     /gene="glgP"
                     /locus_tag="ACHW42_002533"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR8944313.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3105 a         3374 c         3742 g         3314 t
ORIGIN      
        1 cagttacccg taacattttt aattcttgta ttgtgggggc accactttga gcacgactaa
       61 ccatgatcac cacatttacg tcttgatggg cgtatcgggc agcggcaaat ctgcggtcgc
      121 cagtgaagtg gcgcatcaac ttcatgccgc gtttcttgat ggcgatttcc tccatccacg
      181 gcgcaatatc gaaaaaatgg cgtctggcga accgctgaat gacgacgatc gcaaaccctg
      241 gttgcaggcg ctgaacgact ccgcgtttgc tatgcagcgc accaataaag tgtcgctgat
      301 cgcctgttct gcactgaaaa agcactatcg cgacttgctg cgtgaaggta atccgaatct
      361 ctctttcatc tacctgaaag gcgattttga tgtgattgaa agtcgcctga aagcgcgcaa
      421 aggccatttc tttaaaaccc aaatgttggt gacgcagttt gaaacgctgc aggagccggg
      481 tgcggacgaa accgatgtac tggtggtgga tatcgatcaa ccgctggaag gtgttgtggc
      541 aagcaccatt gaggttatta aaaaaggcaa ataagtagtg actacattaa cgcttgtttt
      601 aacagcagta gggtctgttt tactgctgct gtttttagtc atgaaggcgc gtatgcacgc
      661 tttcctggct ttgatggtgg tgtccatggg ggctggcctt ttttccggta tgccgctcga
      721 taaaatcgca gcgacgatgg aaaaagggat gggaggcacc ctcggcttcc tggcggtggt
      781 tgtcgccctg ggagctatgt ttggcaagat cttatatgaa accggcgcag tcgatcagat
      841 tgccgtcaaa atgctcaaat ccttcggtca cagccgcgcg cattatgcca tcggccttgc
      901 ggggctggtc tgtgcgctac cgctgttctt tgaagtggcg attgttctgc tgattagcgt
      961 tgctttctca atggcgcgcc acaccggtac gaacctggtg aagctggtaa tcccattatt
     1021 cgcaggcgtg gcggccgcgg cagcgttcct ggtgcctggg ccagcgccaa tgctgctggc
     1081 atcgcagatg aatgccgact ttggctggat gatcctgatt ggcctgtgtg cggcaattcc
     1141 gggaatgatt attgccgggc cgctgtgggg taacttcatc agccgttacg ttgagctgca
     1201 tattcctgac gacatcagcg aaccgcatct cggcgaaggt aaaatgccat ctttcggatt
     1261 cagcctgtcg ctgatcctgt tgccgctggt gctggtgggg ctgaaaacca ttgccgcgcg
     1321 ttttgtgcca gaaggctcta ccgcttacga atggttcgag tttattggtc atccgtttac
     1381 cgcgattctg gttgcttgtc tggtagcgat ttatggcctg gcaatgcgtc agggcatgcc
     1441 aaaagacaaa gtgatggaga tttgcggtca cgcgctgcaa ccggcgggga tcattctgct
     1501 ggtgattggt gcaggcggcg tgttcaaaca ggtgctggtt gactctggcg taggtccggc
     1561 actgggcgaa gcgttaaccg gcatgggcct gccgattgct atcacctgct tcgtgctggc
     1621 tgctgcagtg cgcatcattc agggggctgc caccgtagcc tgtttaacgg cggtgggact
     1681 ggtgatgcca gtcattgaac aactgaacta ctctggtgcg caaatggcgg cactgtcgat
     1741 ttgtatcgct ggtggttcga ttgttgtcag ccacgttaac gacgctggtt tctggttgtt
     1801 cggtaaattt accggcgcga ccgaagccga aacgctgaaa acctggacca tgatggaaac
     1861 catcctcggc actgttggtg ccatcgttgg gatgattgcg ttccagctgt tgagttaagt
     1921 ttgttcgccc ggtagttgtg acgctaccgg gctcttttcg aaaaactctc ctcgttaccc
     1981 cttcatccac attcgaatgc cgtcgaggaa catctgagtt gccatcatca ccagaatcaa
     2041 tcccatcagg cgttcaagtg cgttcacccc tttctcgccc agcagacgta aaaatagcga
     2101 agactgtagc aggatgacaa aggtgccgcc ccaggccagc agcagagcaa tcaccagatg
     2161 tcccatctga ttcgggtact gatgagacaa caacatcagc gtggcgagaa tagtcggccc
     2221 ggcgactaac ggaattgcca acggcacgat aaatggctct tcacctgccg gaagcccgct
     2281 gctatttcct gaagcgctgg ggaaaatcat cttaatggcg attagaaaca gaatgatgcc
     2341 gccagaaatg gagacggttt ctgctcgcag gctgagaaat gccagaattt tctcacccgc
     2401 aaacaggaac accagcatca ccaggagagc aataagcaac tctcgcacca tgattgcccg
     2461 ccgtcttttc ggttcagtat gtttcagtac ggacatgaaa ataggcaggt ttccgagcgg
     2521 atccataatc aggatcaata aaactgctgc agaaatgatt tcattcataa ctcaaattcc
     2581 ctgataattg ccgcggactt tctgcgtgct aacaaagcag gataagtcgc attactgatg
     2641 gcttcgctat cattgattaa tttcacttgc gactttggca gctttttgta tggtgaaaga
     2701 tgtgccaaga ggagaccggc acatttatac agcacacatc tttgcaggaa aaaacgctta
     2761 tgaaaaatgt tggttttatc ggctggcgcg gtatggtcgg ctccgttctc atgcaacgca
     2821 tggttgaaga gcgcgacttc gacgccattc gccctgtctt cttttctact tctcagcttg
     2881 gccaggctgc gccgtctttt ggcggaacca ctggcacact tcaggatgcc tttgatctag
     2941 aggcgctaaa ggccctcgat atcattgtga cctgtcaggg cggcgattat accaacgaaa
     3001 tctatccaaa gcttcgtgaa agcggatggc aaggttactg gattgacgca gcatcgtctc
     3061 tgcgcatgaa agatgacgcc atcatcattc ttgaccccgt caatcaggac gtcattaccg
     3121 acggattaaa taatggcatc aggacttttg ttggcggtaa ctgtaccgta agcctgatgt
     3181 tgatgtcgtt gggtggttta ttcgccaatg atcttgttga ttgggtgtcc gttgcaacct
     3241 accaagccgc ttccggcggt ggtgcgcgac atatgcgtga gttattaacc cagatgggcc
     3301 atctgtatgg ccatgtggca gatgaactcg cgaccccgtc ctctgctatt ctcgatatcg
     3361 aacgcaaagt cacaacctta acccgtagcg gtgagctgcc ggtggataac tttggcgtgc
     3421 cgctggcggg tagcctgatt ccgtggatcg acaaacagct cgataacggt cagagccgcg
     3481 aagagtggaa agggcaggcg gaaaccaaca agatcctcaa cacatcttcc gtaattccgg
     3541 tagatggttt atgtgtgcgt gtcggggcat tgcgctgcca cagccaggca ttcactatta
     3601 aattgaaaaa agatgtgtct attccgaccg tcgaagaact gctggctgcg cacaatccgt
     3661 gggcgaaagt cgttccgaac gatcgggaaa tcactatgcg tgagctaacc ccagctgccg
     3721 ttaccggcac gctgaccacg ccggtaggcc gcctgcgtaa gctgaatatg ggaccagagt
     3781 tcctgtcagc ctttaccgtg ggcgaccagc tgctgtgggg ggccgcggag ccgctgcgtc
     3841 ggatgcttcg tcaactggcg taatctttat tcattaaatc tggggcgcga tgccgcccct
     3901 gttagtgcgt aatacaggag taagcgcaga tgtttcatga tttaccggga gttaaataga
     3961 gcattggcta ttctttaagg gtggctgaat acatgagtat tcacagcctt acctgaagtg
     4021 aggacgacgc agagaggatg cacagagtgc tgcgccgttc aggtcaaaaa aatgtcacaa
     4081 ccagaagtca aaaatccaat tggatggggt gacacaataa aacaggaaga caagcatgtc
     4141 cgatcgtatc gatagagacg tgattaacgc gctaattgca ggccattttg cggatccttt
     4201 ttccgtactg ggaatgcata aaaccaccgc gggactggaa gtccgtgccc ttttacccga
     4261 cgctaccgat gtgtgggtga ttgaaccgaa aaccgggcgc aaactcgcaa aactggagtg
     4321 tctcgactca cggggattct ttagcggcgt cattccgcga cgtaagaatt ttttccgcta
     4381 tcagttggct gttgtctggc atggtcagca aaacctgatt gatgatcctt accgttttgg
     4441 tccgctaatc caggaaatgg atgcctggct attatctgaa ggtactcacc tgcgcccgta
     4501 tgaaacctta ggcgcgcatg cagatactat ggatggcgtc acaggtacgc gtttctctgt
     4561 ctgggctcca aacgcccgtc gggtctcggt ggttgggcaa ttcaactact gggacggtcg
     4621 ccgtcacccg atgcgcctgc gtaaagagag cggcatctgg gaactgttta tccctggggc
     4681 gcataacggt cagctctata aatacgagat gattgatgcc aatggcaact tgcgtctgaa
     4741 gtccgaccct tatgccttcg aagcgcaaat gcgcccggaa accgcgtctc ttatttgcgg
     4801 gctgccggaa aaggttgtac agactgaaga gcgcaaaaaa gcgaatcagt ttgatgcgcc
     4861 aatctctatt tatgaagttc acctgggttc ctggcgtcgc cacaccgaca acaatttctg
     4921 gttgagctac cgcgagctgg ccgatcaact ggtgccttat gctaaatgga tgggctttac
     4981 ccacctcgaa ctactgccca ttaacgagca tcccttcgat ggcagttggg gttatcagcc
     5041 aaccggcctg tatgcaccaa cccgccgttt tggtactcgc gacgacttcc gttatttcat
     5101 tgatgccgca cacgcagctg gtctgaacgt gattctcgac tgggtgccag gccacttccc
     5161 gactgatgac tttgcgcttg ccgaatttga tggcacgaac ttgtatgaac acagcgatcc
     5221 gcgtgaaggc tatcatcagg actggaacac gctgatctac aactatggtc gccgtgaagt
     5281 cagtaacttc ctcgtcggta acgcgcttta ctggattgaa cgttttggta ttgatgcgct
     5341 gcgcgtcgat gcggtggcgt cgatgattta tcgtgactac agccgtaaag agggggagtg
     5401 gatcccgaac gaatttggcg ggcgcgagaa tcttgaagcg attgaattct tgcgtaatac
     5461 caaccgtatt cttggtgagc aggtttccgg tgcggtgaca atggctgagg agtctaccga
     5521 tttccctggc gtttctcgtc cgcaggatat gggcggtctg ggcttctggt acaagtggaa
     5581 cctcggctgg atgcatgaca ccctggacta catgaagctc gacccgattt atcgtcagta
     5641 tcatcacgat aaactgacct tcgggatgct ctacaactac actgaaaact tcgtcctgcc
     5701 gttgtcgcat gatgaagtgg tccacggtaa aaaatcgatt ctcgaccgca tgccgggcga
     5761 cgcatggcag aaattcgcta acctgcgcgc ttactatggc tggatgtggg catttccggg
     5821 caagaaactg ctgttcatgg ggaacgaatt tgcccagggc cgcgagtgga accatgacgc
     5881 cagcctcgac tggcatctgt tggaaggcgg cgataactgg caccacggtg tccagcgtct
     5941 ggtgcgcgat ctgaacctca cctaccgcca ccataaagca atgcatgaac tggattttga
     6001 cccgtacggc tttgaatggc tggtggtgga tgacaaagaa cgctcggtgc tgatctttgt
     6061 gcgtcgcgat aaagagggta acgaaattat cgttgccagt aactttacgc cggtgccgcg
     6121 tcatgattat cgcttcggca ttaatcagcc aggtaaatgg cgtgaaatcc tcaataccga
     6181 ttccatacac tatcacggca gtaatgcagg caatggcggc acggtacaca gcgatgagat
     6241 tgccagccac ggtcgtcagc attcactaag cctgacgcta ccaccgctgg ccactatctg
     6301 gctggttcgg gaggcagaat gacacaactc gccattggca aacccgctcc cctcggcgcg
     6361 cattacgacg gtcagggcgt caacttcaca cttttctccg ctcatgccga gcgggtagaa
     6421 ctgtgtgtct ttgacgccaa tggccaggaa catcgctatg acttgccagg gaacagtggc
     6481 gacatttggc acggttatct gccggatgcg cgcccgggtt tgcgttatgg ttatcgcgtt
     6541 catggcccct ggcaacccgc cgaggggcat cgctttaacc cggcgaagtt gttgattgat
     6601 ccttgcgcgc ggcaaattga cggggagttt aaagataacc cgctgctgca cgccggtcat
     6661 aatgaacctg actatcgcga caacgccgcc attgcgccga aatgcgtagt ggtggttgat
     6721 cactatgact gggaagatga cgccccgccg cgcacgccgt ggggcagcac catcatttat
     6781 gaagcccatg tcaaaggatt aacgtacttg cacccggaga tcccggtcga gatccgtggc
     6841 acttataaag ccctcgggca tccggtgatg atcaactatt tgaaacaatt gggcattacc
     6901 gcgctggaac tgctgccagt ggcgcagttt gccagtgaac cacgtctgca acgcatgggg
     6961 ctaagtaact actggggtta caacccggtg gcgatgtttg cgctgcatcc ggcgtatgcc
     7021 tgctcgccag aaacggcgct ggatgagttt cgcgatgcaa tcaaagcact gcataaagcg
     7081 ggtatcgaag tcattcttga tatcgtgctc aaccatagtg cggaactgga cctcgacggc
     7141 ccgttattct cgctgcgtgg gatcgataac cgtagctatt attggataag agaagacggc
     7201 gattatcaca actggaccgg ttgcggcaac acgctcaatt tgagtcatcc ggcggtggtg
     7261 gattatgcca gcgcctgcct gcgttattgg gtagaaacct gccacgtcga tggtttccgc
     7321 tttgatctgg cggcagtcat gggccgtacg ccagagttcc gtcaggatgc gccgttgttt
     7381 accgctatcc agaactgccc ggtgctctcg caggtgaagt taattgctga accgtgggat
     7441 atcgctccta gtggttatca ggtgggaaat ttcccgccgc tgtttgccga gtggaacgat
     7501 catttccgcg atgctgcccg tcgtttctgg ctacattatg atttgcctct gggggcgttt
     7561 gccgggcgtt ttgctgcctc cagcgatgtt tttaaacgta atggtcgtct gccgagtgcc
     7621 gcgattaatc tcgtcaccgc gcatgacggt tttacgcttc gcgactgcgt ttgcttcaac
     7681 cataaacaca atgaagcaaa cggagaagaa aatcgcgacg ggaccaacaa caattacagt
     7741 aacaatcatg gtaaagaagg gttaggcggt actcttgacc tggttgaacg gcggcgcgac
     7801 agcattcatg ccctgttaac aacgttgttg ctctcccagg gcacgccgat gttactggcc
     7861 ggtgacgaac atggtcacag ccagcatggc aataacaatg cctactgtca ggataaccaa
     7921 ttaaactggt tggactggtc gcaggcaagc agtggtttaa ccgcatttac cgccgcgtta
     7981 atccatctgc gcaagcgcat tcccgctttg gtggagaatc gctggtggga agaaggtgac
     8041 ggcaatgtcc gttggctaaa tcgatatgct caacctttaa gcacggatga gtggcaaaac
     8101 gggccgaaac agctgcaaat tctgctctcg gatcgctttt tgatcgcaat taacgccacg
     8161 cttgaggtaa cagagattgt tttacctgct ggggagtggc acgccattcc cccattcgct
     8221 ggagaggata acccagtgat tacggttgtc tggcagggac ctgcacacgg attgtgtgtg
     8281 ttccagagat gataaaaaag gagttagtca tggttagttt agagaagaac gatcacttaa
     8341 tgttggcgcg ccagctgcca ttgaaatctg ttgccctgat actggcggga ggacgtggta
     8401 cccgcctgaa ggatttaacc aataagcgag caaaaccggc cgtacacttc ggcggtaagt
     8461 tccgcattat tgactttgcg ctgtctaact gcatcaactc tgggatccgt cgtatgggcg
     8521 tgatcaccca gtaccagtcc cacactctgg tgcagcacat tcagcgcggc tggtcattct
     8581 tcaatgaaga aatgaacgag tttgtcgatc tgctgccagc acagcagaga atgaaagggg
     8641 aaaactggta tcgcggcacc gcagatgcgg tcacccaaaa cctcgacatt atccgccgtt
     8701 ataaagcgga atacgtggtg atcctggcgg gcgaccatat ctacaagcaa gactactcgc
     8761 gtatgcttat cgatcacgtc gaaaaaggcg cacgttgcac cgttgcttgt atgccagtac
     8821 cgattgaaga agcctccgca tttggcgtta tggcggttga tgagaacgat aaaattatcg
     8881 aattcgttga aaaacctgct aacccaccgt caatgccgaa cgatccgagc aaatctctgg
     8941 cgagtatggg tatctacgtc tttgacgccg actatctgta tgaactgctg gaagaagacg
     9001 atcgtgatga gaactccagc cacgactttg gcaaagattt gattcccaag atcaccgaag
     9061 ccggtctggc ctatgcgcac ccgttcccgc tctcttgcgt acaatccgac ccggatgccg
     9121 agccgtactg gcgcgatgtg ggtacgctgg aagcttactg gaaagcgaat ctcgatctgg
     9181 cctctgtggt gccggaactg gatatgtacg atcgcaattg gccaattcgc acctacaatg
     9241 aatcattacc gccagcgaaa ttcgtgcagg atcgctccgg tagccacggg atgaccctta
     9301 actcactggt atccggcggt tgtgtgatct ccggttcggt ggtggtgcag tccgttctgt
     9361 tctcgcgcgt tcgcgtgaat tcattctgca acattgattc cgccgtattg ttaccggaag
     9421 tatgggtagg tcgctcgtgc cgtctgcgcc gctgcgtcat cgatcgtgct tgtgttatcc
     9481 cggaaggcat ggtgattggt gaaaacgcag aggaagatgc acgtcgtttc tatcgttcag
     9541 aagaaggcat cgtgctggta acgcgcgaaa tgctacggaa gttagggcat aaacaggagc
     9601 gataatgcag gttttacatg tatgttcaga gatgttcccg ctgcttaaaa ccggcggtct
     9661 ggctgatgtt attggggcat tacccgcagc acaaatcgca gacggcgttg acgctcgcgt
     9721 actgttgcct gcatttcccg atattcgccg tggcgtgacc gatgcgcagg tagtatcccg
     9781 tcgtgatact ttcgccgggc atatcacgct gttgttcggt cattacaacg gggttggcat
     9841 atacctgatt gacgcgccgc atctctatga tcgtccggga agcccgtatc acgataccaa
     9901 cttatttgcc tataccgaca acgtattgcg ttttgcgctg ctggggtggg ttggggcaga
     9961 aatggccagc gggcttgacc cattctggcg tcctgatgtg gtgcatgtgc acgactggca
    10021 tgcaggtctt gcgcctgcgt atctggcggc gcgcgggcgt cctgcgaagt cggtgtttac
    10081 tgtgcacaac ctggcctatc aaggcatgtt ttatgcacat cacatgaatg acatccaatt
    10141 gccatggtca ttctttaata ttcatgggct ggaattcaac ggacaaatct ctttcctgaa
    10201 ggccggtctg tactatgccg atcacattac ggcggtcagt ccaacctacg ctcgcgagat
    10261 caccgaaccg cagtttgcct acggtatgga aggtctgttg caacagcgtc accgtgaagg
    10321 gcgtctttca ggcgtactga acggcgtgga cgagaaaatc tggagtccag agacggactt
    10381 actgttggcc tcgcgttaca cccgcgatac gttggaagat aaagcggaaa ataagagcca
    10441 gttacaaatc gcaatggggc ttaaggttga cgataaagtg ccgctttttg cagtggtgag
    10501 ccgtctgacc agccagaaag gtctcgacct ggtgctggaa gccttaccgg ggcttctgga
    10561 gcagggcggg cagctggcgc tactcggcgc gggcgatccg gtgctgcagg aaggtttcct
    10621 tgcggcggca gcggaatacc ccggtcaggt gggcgttcag attggctatc acgaagcatt
    10681 ttcgcatcgc attatgggcg gcgcggacgt cattctggtg cccagccgtt ttgaaccgtg
    10741 cggcttaacg caactttatg gattgaagta cggtacgctg ccgttagtgc ggcgcaccgg
    10801 tgggcttgct gatacggttt ctgactgttc tcttgagaac cttgcagatg gcgtcgccag
    10861 tgggtttgtc tttgaagata gtaatgcctg gtcgctgtta cgggctattc ggcgtgcttt
    10921 tgtactgtgg tcccgtcctt cactgtggcg gtttgtgcaa cgtcaggcta tggcaatgga
    10981 ttttagctgg caggtcgcgg cgaagtcgta ccgtgagctt tactatcgct tgaaatagtt
    11041 ttcaggaaac gcctatatga atgctccgtt tacatattca tcgcccacgc ttagcgtaga
    11101 agctcttaag cactctatcg cttacaagct gatgtttacg attggaaagg acccggtcgt
    11161 cgccaataaa catgaatggc tgaacgcaac gttatttgct gtgcgcgatc gtctcgtgga
    11221 gcgctggtta cgttcaaacc gtgcccagtt gtcgcaagaa actcgtcagg tttactacct
    11281 gtcgatggag tttttgattg gccgtacgct ctccaacgcc atgttgtcgc taggaattta
    11341 cgaagatgta cagggcgcac tggaagcgat ggggttaaat ctcgaagagc tgattgatga
    11401 agaaaatgac ccaggcctcg gtaacggtgg cctgggacgt ctggcggctt gcttccttga
    11461 ttctctggcg acgttagggt tgccggggcg cggttacggc atccgctatg actacggtat
    11521 gttcaagcag aacatcgtta acggtagcca gaaagagtcg ccagactact ggctggaata
    11581 cggtaacccg tgggaattca aacgccacaa cacgcgctat aaagtccgtt ttggtggtcg
    11641 cattcagcag gaaggtaaaa aaacgcgctg gattgaaacc gaagagattc tgggagtcgc
    11701 ttacgatcag ataatccctg gttacgacac cgacgcgacc aacacgctgc gtttgtggag
    11761 tgcgcaagcc agtagcgaaa ttaacctcgg taaattcaac cagggtgact acttcgcggc
    11821 agtggaagat aaaaaccact ccgagaacgt atctcgcgta ctgtatccgg atgactccac
    11881 ctactccggg cgtgagctgc gcctgcgtca ggaatacttc ttggtttcct cgaccattca
    11941 ggacatttta agccgtcatt atcagttgca taaaacctac gataacctgg cggataaaat
    12001 cgcgattcat ctcaatgata cccatccggt actgtcgatt cctgagatga tgcgtctgct
    12061 gatcgatgag caccaattta gctgggacga cgcgtttgag gtgtgttgtc aggtcttctc
    12121 ctacactaac cacacgctga tgagcgaggc gctggaaacc tggccggttg atatgctggg
    12181 taaaattctg ccgcgtcacc tgcagatcat ctttgaaatc aacgactatt tcctgaaaac
    12241 cttgcaggaa cagtatccga acgataccga tctgctggga cgggcgtcga tcattgatga
    12301 atccaacggt cgtcgtgtgc gtatggcctg gctggcggtt gttgtgagcc acaaagttaa
    12361 cggtgtatcg gaactgcact ctaatctgat ggtgcaatcg ttgtttgccg actttgcgaa
    12421 aatcttcccg ggtcgtttca ccaacgtcac caacggtgtg acgccgcgtc gctggctggc
    12481 ggtagcgaac ccatcgcttt cagccgtgct ggacgaacac ctgggccgta actggcgcac
    12541 cgaccttagc ctgcttaatg agctgcaaca acactgtgat ttcccaatgg ttaatcacgc
    12601 tgtgcatcag gcgaagctgg agaacaaaaa gcgtctggca gagtatatcg cccagcagct
    12661 gaatgtggtg gtgaatccaa aggcgttgtt cgatgtacaa atcaaacgta ttcacgaata
    12721 caaacgtcaa ttgatgaatg tgttgcatgt gattacccgc tataaccgca tcaaggccga
    12781 cccggatgcg aagtgggtac cgcgcgtgaa tatttttggc ggtaaggcgg cttcggccta
    12841 ttacatggcg aagcacatta ttcatttgat caatgacgta gcgaaagtga tcaacaacga
    12901 tccgcagatt ggcgataagc tgaaagtcgt gttcatcccg aactacagcg ttagcctggc
    12961 gcagttgatc attccggcgg cagatctgtc tgaacagatt tcgctggcag ggacggaagc
    13021 ttccggcacc agtaacatga agtttgcgct taacggtgcg ctgactatcg gtacgttgga
    13081 cggtgcgaat gtcgagatgc tggatcatgt cggtgctgac aatatcttta tttttggtaa
    13141 cacagcggaa gaagtggaag aactgcgtcg tcagggctac aaaccgcgtg aatactacga
    13201 gaaagatgag gagctgcatc aggtgctgac gcaaatcggc agcggtgtat tcagtccgga
    13261 agatccgggt cgctatcgcg atctggttga ttcgctgatc aacttcggcg atcactacca
    13321 ggtactggcg gattatcgca gctatgtcga ttgtcaggat aaagtcgacg aactctacga
    13381 gcttcaggaa gagtggaccg caaaagcgat gctgaacatt gccaatatgg gctacttctc
    13441 ttctgaccgt actatcaaag agtacgctga tcatatctgg catatcgatc cggtgagatt
    13501 gtaatttcac taataaatag aacggggcca aaggg
//
LOCUS       DAZSNM010000106        13411 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZSNM010000106 DAZSNM010000000
VERSION     DAZSNM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13411
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.106"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(24..278)
                     /locus_tag="ACHW42_002534"
     CDS             complement(24..278)
                     /locus_tag="ACHW42_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR8944314.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="ACHW42_002535"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="ACHW42_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR8944315.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="ACHW42_002536"
     CDS             1228..2694
                     /locus_tag="ACHW42_002536"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR8944316.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHW42_002537"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHW42_002537"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR8944317.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHW42_002538"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHW42_002538"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR8944318.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="ACHW42_002539"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="ACHW42_002539"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR8944319.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="ACHW42_002540"
     CDS             5709..7265
                     /locus_tag="ACHW42_002540"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR8944320.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="ACHW42_002541"
     CDS             7347..9161
                     /locus_tag="ACHW42_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR8944321.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="ACHW42_002542"
     CDS             9162..10256
                     /locus_tag="ACHW42_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR8944322.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="ACHW42_002543"
     CDS             10256..11281
                     /locus_tag="ACHW42_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8944323.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="ACHW42_002544"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="ACHW42_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR8944324.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="ACHW42_002545"
     CDS             complement(12876..13220)
                     /locus_tag="ACHW42_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR8944325.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
BASE COUNT         3222 a         3321 c         3352 g         3516 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccatgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta a
//
LOCUS       DAZSNM010000107        13384 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZSNM010000107 DAZSNM010000000
VERSION     DAZSNM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13384
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.107"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..2119
                     /locus_tag="ACHW42_002546"
                     /pseudo
     CDS             1..2119
                     /locus_tag="ACHW42_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            2129..5775
                     /locus_tag="ACHW42_002547"
                     /pseudo
     CDS             2129..5775
                     /locus_tag="ACHW42_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            5836..6243
                     /locus_tag="ACHW42_002548"
     CDS             5836..6243
                     /locus_tag="ACHW42_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944326.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            6785..7873
                     /locus_tag="ACHW42_002549"
     CDS             6785..7873
                     /locus_tag="ACHW42_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFR8944327.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            7884..10163
                     /locus_tag="ACHW42_002550"
     CDS             7884..10163
                     /locus_tag="ACHW42_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFR8944328.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            10156..11292
                     /locus_tag="ACHW42_002551"
     CDS             10156..11292
                     /locus_tag="ACHW42_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR8944329.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            11289..13292
                     /locus_tag="ACHW42_002552"
     CDS             11289..13292
                     /locus_tag="ACHW42_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944330.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3278 a         3521 c         3459 g         3126 t
ORIGIN      
        1 gttagccatg aagaaagtac taccaacctg ttggcaagga tgggttttca acgacggatc
       61 gctggtacat atattaaagc tcccgaagcg gtagttgagg catggctaaa tgaagattat
      121 tcaaccttac taagtgaatt taaggtgttt cattcaccta ccgggcatta ttggcagttg
      181 gggattttga caacattgcc gctggaggct ggagaaagca gtaaaagcat ggaatgccct
      241 tactctatct ccacataccg ataccgaata cgccatgtta cattttggac tcaaaggttt
      301 acctggttta gttaactcaa ttacacgcta tccacaggaa gccttgccca tcacgaatta
      361 cttcgcagcg agtgagctgg ctcctgccgt cgcccgtgcc ttcaacaaac tcaaaacgct
      421 acgcgaaaac gcccgcacct ggctgttgaa atacccggaa catgccctta ccggcctgct
      481 gcctgcggcg ctcggcaaag ccggtgaagc acaggataac gcccgcgctg ccttgcgtat
      541 gcttaccgaa aacggtcatc agccattact gcaagaaatc gcccggcgtt ataaccagtc
      601 ggaagtaaca gacgcggtga acgctctgct tgcgctcgat cccttagata atcacccgac
      661 aaaaatcccc actcttccga ccttttatca gccatcgatc tggacgcgcc cggtattaaa
      721 agcaaacgcc caatcactgc cagataacgc cctcctccac ctcggcgaaa tgctccgctt
      781 ccctcaggaa gaggctctgt atccgggatt attgcaggtg aaagacgcct gtaccgctga
      841 ctctctggct gaatttgcct gggatctgtt taccgcctgg cagaccgctg gcgcgccgtc
      901 gaaagagagt tgggcgttca ctgcgttagg cgttctcggt aacgatgaca ccgcccgcaa
      961 actgacgcca ttaatacgcg cctggcctgg tgaatcccag cataaacgcg ccaccgttgg
     1021 gttggatatt ctcgccgcta tcggtagtga tatcgccctg atgcagctta acggcattgc
     1081 tcagaaactg aaattcaaag cattacagga gcgggcaaaa gaaaagattg ccgacattgc
     1141 cgagagccgc gaactcacgg tagcggagct tgaagatcgg ttagcaccgg atctcggtct
     1201 ggatgataac ggttcgttgc tgctggattt tggcccacgc cagttcaccg tcagctttga
     1261 tgaaacctta aaaccgtttg tgcgtgatgt ttccggcagc cgcctgaaag acctgccaaa
     1321 accaaacaaa agcgattatg agtcacaggc gaacgatgct gttaaccgct acaaattgct
     1381 gaaaaaagat gcgcgtaccg tcgcggccca gcaggtagca aggcttgaat ccgccatgtg
     1441 cctgcgtcgc cgctggtcgc cggaaaactt ccaactcttc ctggttgtgc atccgctggt
     1501 tcgccactta acccgccgtc tgatttgggg cgtttatagc gccgacaacc agctactggc
     1561 ctgtttccgg gtagcggaag ataacagcta cagcaccgct gacgatgatc ttttcaccct
     1621 gccggaaggc gatatctcta tcggcattcc tcacgttctg gaaatatcac cgacggatgc
     1681 tgtcgccttt ggtcagcttt ttgccgacta cgaactgcta ccaccgttcc gtcagcttga
     1741 ccgtaacagt tacgccctga cagaagccga gcgcaacgcc agcgaactga cccgctgggc
     1801 aggcagaaaa tgcccgagcg gccgggtcat ggggctggca aataaaggct ggataaaggg
     1861 cgaaccacag gatggaggct ggatgatcaa acctctcggt cgctggtcgt taatcatgga
     1921 aatcgacgaa ggttttgcgg tgggtatatc accagccgaa cttagcgctg agcagctctt
     1981 aagcaagctg tggctatggg aaggcaaagc ggaaagctat ggctggggga gtaattcaac
     2041 acaggaagcg cagttctccg tactcgatgc catcaccgcc agcgagctaa ttaacgatat
     2101 tgaagccctg tttgaataag gaaagagcat ggacaaggaa ttaccgtggc tggcggataa
     2161 cgcccaactg gaactgaaat ataaaaaagg caaaacacca ctcagtcatc gcaattggcc
     2221 gggcgaacca gtgcctgtta tcactgaaag catcattcag acactgggtg atgaattgct
     2281 acaaaaagct gagaagaaaa aaaacattgt ctggcgttat gaaaattttt caccggagtg
     2341 gcagtccgcc attacgcagg ccatcaactt gatcggcgaa cacaaaccct cagtcccggc
     2401 ccgcacaatg gcggcactag cctttatcgc gcaaaatgac aaccaacagt tgctcgacga
     2461 aatcgtccaa caagaggggc tggaatatgc gactgaggtg gtgattgcac gccagtttat
     2521 tgcacggtgt tatgagagtg atcctctggt agtcacattg cagtatcaga acgaggacta
     2581 tggctatggt tatcgctcag aaacctataa cgaattcgat ctccgactgc gtaaacacct
     2641 ctctctggca gaggaaagct gctggcagcg ttgcgccgac aaactcattg ccgcactgcc
     2701 aggaataacc aaagttcgcc gcccttttat tgcgctgatc ctccccgaaa aaccagaaat
     2761 cgccaatgag ttggtaagcc ttgaatgccc gcggactcat tttcattcta aggagtggtt
     2821 aaaagttgtt gctactgacc ccaaagcggt gagaaaactc gaacgctact ggagccagga
     2881 tatatttagc gatcgagaag ccagctacat gtcgcatgaa aaccacttcg gctacgcggc
     2941 ctgcgctgcc cttttgcgcg aacaaggact ggcagtcgtt ccgcgcctcg cgatatatgc
     3001 ccataaagaa gattgcggca gtctgctggt acaaattaac catccgcaag tcatccgcac
     3061 cttgctgtta gttgctgata aaaacaaacc cagcctgcaa cgtgtggcta aatacagtaa
     3121 aaacttcccc catgcgacac tcgccgcact ggcagaactg ctggcgttaa aagaaccacc
     3181 agcccgccca ggttatccaa tcatcgaaga caaaaagctg cctgcacagc aaaaagctcg
     3241 agatgaatac tggcgtacgc tgttacaaac gctgatggca tcgcagccac aactggcagc
     3301 agaagtgatg ccgtggttaa gtactcaagc ccgggcagtg ctgaatagtt atttatcggc
     3361 accgcccaaa ccagttattg atagcaccga taacagccaa atgcctgaaa tcctcgtttc
     3421 accaccatgg cgtgggagaa agaaaaagac ttccatacgt cacttttcgc tgcctgatct
     3481 tacgcttgca ccgcaaacgt acaggtcgcc gaataaacac tggacgccgg aacagcaaac
     3541 tgcaattgac cattttagcg ccatgtcttt tgacgaacgt cttgctacga gaggagcgga
     3601 tatcttcttg cgggaattag gttttgacgt tcatagcctg gaatttaata agcgtcttct
     3661 taatggacag ttggatgcct ttgatgctca gcaactgcaa gaatacacat caataggact
     3721 attgcgtact tatcacgcgc atcaggccaa atctaaacag taccgggaag acgccaccaa
     3781 tgcattactg gcacaagata gcattgcgtt gatgggaggg tggcgaactt ttcagcacgg
     3841 attttactat ggtaatgaac agcgttgggg gatttggaat ctgtacctta tcgcgcagat
     3901 gccgcgcgaa atggcggtat cgtgctggca acaaattgtc gaggctgatt ttcattacac
     3961 cggggtggaa tatttgttaa gtgtgctggg taccgatgct ttgcctggat taaacaccgc
     4021 ctttgcccga catccaaaag agatatttcc gctgctgatt catttcggcg caacagaact
     4081 ggccctgccc attgcccgcg tctggcacct ttttgcgggc cagcgtaatc tggctcgcca
     4141 gtggatttta caatggccgg aacatacggc tactgcactt atccctcttg tcttcaccaa
     4201 atctagcgat aaaagcgaag ctgcattact tgccctgcgt ttactttacg aacatgggca
     4261 tggcgaatgg ctacaaactg tagccaatcg ttggcagcgt acagatctat ggcctgccct
     4321 ggaacaatta cttaaacaga gtccaataga gatttacccg gcacgcattc caaaagcccc
     4381 tgatttctgg catccgcaaa tgtggtccag gccgcgcctt atcactaata atcaacctgt
     4441 taccgatgac gctctggaaa ttatcggcga aatgctgcgc tttacccagg ggggacgttt
     4501 ttatagcggg ctggaacaac tgaaaacgtt ctgccagcca caaacgctgg cagcttttgc
     4561 ctgggatctc ttcactgcgt ggcaacaagc tggtgccccc gcaaaagaca actgggcatt
     4621 tctggcgtta agtctctttg gtgacgaaag cacggcacgg gatctaacga cacatatcct
     4681 cgcctggcca caagaaggca aatctgcccg tgcggtcagt ggcatgaaca tccttaccct
     4741 gatgaataat gatatggcgc tgatgcagct gcatcatata tcgcaacggg cgaaatcccg
     4801 ccccttacgt gataacgcgg cggaatttct tcaggtagtc gcagaaaatc gcgggctaag
     4861 ccaggaagag ttagcggaca gattagtccc aacactgggc cttaatgatc cgcaggcgtt
     4921 gagttttgat tttggtcccc ggcagtttac cgttcgcttc gatgaaaacc tcaacccggt
     4981 tatctttgat cagcaaaacg ttcgccagaa aagtgttccc cggttgcgcg ccgatgacga
     5041 tcaactgaaa gcgcccgagg cactggcccg actaaaaggg ttaaaaaaag atgctactca
     5101 ggtgagcaaa aacctgctcc cgcgtcttga agctgcccta cgtaccaccc gacgctggtc
     5161 gctggcagat tttcattctc tgtttgttaa tcatcccttt acccgtctgg ttacccagcg
     5221 attaatatgg ggggttatcc ggcaaatgaa ccgcgtcgtt tactcaacgc ctttcgtgtg
     5281 gccgcagagg gggagttctg caatgcgcaa gatgagccaa ttgacctgcc tgcggacgct
     5341 ctgattggca ttgcccaccc gttagaaatg acagcagaaa tgcgcagtga atttgcacag
     5401 ctttttgccg attacgaaat tatgccgcct tttcgccagt tgtcgcgccg cacggtgctg
     5461 ctcacacctg acgagtcaac cagtaacagc ctgactcgct gggaaggtaa atccgctacc
     5521 gttgggcaac ttatgggaat gcgatacaaa ggctgggagt caggttatga ggacgcattt
     5581 gtctatgacc tgggtgagta ccggctggtc cttaagtttt cacccggttt taaccactac
     5641 aatgttgata gcaaagcgct aatgagcttc cgttctcttc gagtgtaccg tgacaataaa
     5701 tccgtcactt ttgccgaact tgatgtgttt gatttgagtg aggcgttaag cgcacctgac
     5761 gtcattttcc attaaaacaa agcgggcagt gatgcgactg cccgttatct acacgactta
     5821 ccagcgggga aagcgatgat cgtgcaaaaa gagctggttg ctatttacga ttatgaggtc
     5881 cctgtacctg aagatccgtt ttccttcaga cttgagatcc ataaatgctc tgaattattt
     5941 acaggttccg tctaccgact ggaacggttc cggctacgtc caacatttca tcaacgtgat
     6001 cgagaggatg ttgacccgct aataaatgat gcgttgattt atataagaga tgagtttatt
     6061 gatgagcaaa aattacgagg cgaatcacct gaacctgtaa tagtaatttt taattgtaaa
     6121 ctacagaata tattcaacca acaaatagaa ttatatactc tagataattc aagtcgcagc
     6181 acatgcaact tgaagtatga cgagtatagc tttattaaag gggggaacgg tattccccct
     6241 tagatgcgtt tcaatttgcc cgttacttca acaaatcaat caaccgtaac agagccacta
     6301 atagctccag tacagcaatg agcacggtta acttttgtgt cagcgtgtcc atccgctctc
     6361 cttattaagg agcacaacac cagcacctta cccttacact gcctgtcgat actggtttac
     6421 gtttgttgag tcgccatgtt gcactccgga tcagagcact acctgcggca ccacccgtcc
     6481 ggtagccagg acttgaaata ggttcgatcc ggcgcaactt cccaacagtc aggattataa
     6541 atctcattat cttaattgca acactgtcca gaggtggtta taattcgttc gttggaacac
     6601 gatgttgcac aggcagtggt gttaggcctg aaaatagtaa ataaaagaac ctgcctcacc
     6661 agcagttttt tttatttact gtgatctgct ttccagatat ttttcgctca aacaactaat
     6721 gcgccaaaca tttattgcgc gtaaaatatc gtttatttca ttaatacatt tcagggatga
     6781 atatatgtca ccgcagaata atcatctgca gcgtccgcct gctgctgtgt tatacgccga
     6841 tgaactggca aaattaaaac aaaatgataa cgcaccttgc ccgcccggtt ggcagttaag
     6901 tttgcctgcg gcccgtgctt ttatccttgg cgatagcgcg caaaatatta gccgtaaagt
     6961 ggtgattagt ccctccgctg tcgaacgtat gttagtcact cttgccaccg gacgcggttt
     7021 gatgttggtg ggggaacctg gcaccgcaaa atctctcctg tctgaattac tggcaacctc
     7081 tattagcggc gatgccgggt taaccatcca gggcggcgca tctactaccg aagatcaaat
     7141 caagtacggc tggaactacg ccctgcttat caatcatggt ccatcaacgg aagcgctggt
     7201 ccctgccccg ctttaccagg gaatgcgcga cggcaaaatc gtccgttttg aagagataac
     7261 gcgtacaccg ctggaagtgc aagactgtct gttaggtatg ctttctgaca gggtgatgac
     7321 ggtgccggaa ctcactggtg aagccagtca gctctatgcg cgagaaggct ttaatattat
     7381 tgccactgcc aatacccgcg atcgcggcgt caatgaaatg agtgctgcgc taaagcgtcg
     7441 cttcgatttt gaaactgtct ttccgattat ggatttcgcc caggagctgg agcttgtcgc
     7501 cagtgcttcg gcgcgtttgc tggcccatag cggtattccg cataaagtgc ctgacgcggt
     7561 actggagtta ctggtccgca ccttccgcga tctgcgtgcc aacggcgaaa agaaaacgtc
     7621 aatggataca ctaacagcga ttatgtccac cgctgaagcc gtgaacgtgg cccacgccgt
     7681 aggcgtccgc gcctggtttt tagcgaatcg cgcaggtgag ccagccgacc tggtggactg
     7741 cattgccgga accatcgtca aagataacga agaagatcgc gctcgtctgc gccgttactt
     7801 cgaacagcgt gtcgccacac ataaagaagc tcactggcag gcttattatc aagcccgcca
     7861 ccgcctgccg tgaggaaaga tgcatgagcg agccgttaat tgtcggcatc cggcatcata
     7921 gtccggcctg cgcccggctg gtgaaatcgt taatcgaaag ccagcggcca cgatacgtgt
     7981 tgattgaagg ccctgctgat tttaatgacc gggtagacga actgttttta gcccaccagc
     8041 ttccggtagc tatttacagt tattgccagt atcaggacgg tgcagccccc gggcgtggtg
     8101 cctggacgcc atttgctgaa ttttcgccgg agtggcaggc gctacaagcc gatcgtcgta
     8161 ttcaggcaca aacttacttc atcgatttgc cttgctgggc gcagagtgaa gaagaggacg
     8221 attcgcctga tacgcaagag gaaagccaga ccttgctgct gcgtgccacc cgcatggata
     8281 acagcgacac cctgtgggat cacttgttcg aagatgaaag ccagcaaact gcattaccct
     8341 ctgcactggc gcactatttt gcccaactgc ggggcgactc ccccggcgat gctctaaatc
     8401 gtcagcgcga agcctttatg gcccgctgga ttggatgggc gatgcagcaa aataatggcg
     8461 acgtgttagt tgtctgcggt ggctggcacg ctccggcact ggcaaaaatg tggcgcgaat
     8521 gtccgcaggt aattaacacg ccagaattgc cgtcgctggc agatgccgtt acaggttgtt
     8581 atctcacccc ctacagtgaa aagcgccttg atgtgctggc aggatacctt tcaggaatgc
     8641 ctgccccggt ctggcaaaac tggtgctggc agtggggctt acagcaggcg ggagaacaac
     8701 tgctaaaaac ggttctcacc cgtttgcgcc agcacaagtt gcctgcttcg accgcggata
     8761 tggctgccgc tcatctgcat gcgatggcac tggcacagtt gcgcggtcat acactaccgt
     8821 tacgcactga ctggctggat gccatagcag gatcgctgat taaagaagcc ctgaatgcgc
     8881 cgttgccgtg gagctatcgc ggcgttattc atcccgatac cgatccgatt ctgctaacgt
     8941 tgatagacac attagcgggt gacggattcg gtaaacttgc cccttctacg ccacaaccgc
     9001 ctctgccaaa agatgtcact tgcgaactgg aacgtaccgc aatctctctt ccggcggagc
     9061 ttaccttaaa tcgctttaac cccaatggac tagcgcaaag tcaggtgtta catcggctgg
     9121 caatactgga gatcccaggg attgtacgcc agcagggaag cacactgaca cttgcaggca
     9181 acggtgaaga acgctggaaa ttaacccgcc cgcttagcca gcatgcggca ttgattgagg
     9241 ccgcctgctt tggtgccaca ctcctggaag ccgcacgcca taaattagaa gccgatatgc
     9301 tggacgcggg tggaatcggc agcattacca catgtcttag ccaagcggcg tcagcgggtc
     9361 tggcgtcctt cagtcaacaa ttactggagc aactcacatt attaatcgcc caggaaaatc
     9421 aatttgccga aatgggccag gcgctggaag tgctatatgc cttatggcgg ctggatgaaa
     9481 ttagcggtat gcaaggcgcg cagatattac aaacgacgtt atgcgcggct atcgatcgca
     9541 cgctgtggct gtgtgaatcc aacggcagac cggatgaaaa ggagtttcac gctcacttgc
     9601 atagctggca agcgttgtgt catatcctgc gcgatctaca tagcggtgtg caactccccg
     9661 gcgtttcgct ttctgcggcg gtagccttgc tggagcgaca cagtcaggcg attcatgtcc
     9721 tggcactgga tcgcggcgcg actcttggtg cgctgatgcg tctggaacat cccaacgcta
     9781 gtgccgaagc ggcgctgacg atgctggcgc agttatcccc ggcacaatcc ggcgaggcgc
     9841 tgcacggttt gctggcatta gcccgtcatc aactggcctg tcagccggca tttatcgccg
     9901 gtttcagcag tcatttaaat caactaagtg atgccgattt taccaatgcc ctgcccgatt
     9961 tacgcgcggc aatggcctgg ctaccgccac gagaacgcgg gacgctggcg catcaggtgc
    10021 ttgagcatta tcaactggcg caacttcccg tttcggcact gcaaatgccg ttgcattgtc
    10081 caccgcaagc cattgcacat catcaacaac tcgaacagca ggcactggca tcgctgcaac
    10141 actggggagt tttccatgtc tgaactgaac gatcttctga ccacccgtga gctacaacgc
    10201 tggcgattaa ttcttggcga agcggcagaa acgacgcttt gtgggctgga tgacaacgcc
    10261 cggcagatag atcacgcgct ggagtggctg tatgggcgcg atcctgaacg gctccagcgt
    10321 ggtgaacgct ctagtggatt aggtggctca aatctcacca ctcctgagtg gatcaacagt
    10381 attcacacgc tgtttccgca acaggtgatt gagcggctgg aaagcgatgc cgtactgcgc
    10441 tacggcattg aagatgtggt gacaaatctc gacgtgctgg aacgtatgca gccttccgaa
    10501 agcctgctac gcgccgtttt gcacaccaaa catctgatga accccgaagt actggctgcc
    10561 gcccgcagga ttgtgcgcca ggttgtggaa gaaattatgg cgcgactggc aaaggaagtt
    10621 cgtcaggctt tttctggtgt ccgcgatcgt cgtcgccgct catttattcc actggcgcga
    10681 aactttgatt tcaaaagtac tctgcgcgcc aacctgcaac actggcaccc gcaacacggc
    10741 aagttgtata tcgaattccc ccgctttaac agccgcatta aacgccaaag cgagcaatgg
    10801 caactggtct tactggttga tcaaagcgga tcgatggtcg attcggtgat ccactctgcg
    10861 gtgatggcgg cctgtttatg gcagttaccc ggcattcgta cccatctggt ggcgtttgac
    10921 acaagcgtcg ttgatctcac ggcagacgtt gccgatccgg tagagttatt aatgaaagtg
    10981 caattaggcg gcggaaccaa tatcgccagt gccatggagt atggtcggca acttattgaa
    11041 caacctgcaa aaagcgtcat tatcctcgtg agcgattttt atgaaggggg ttcatcatca
    11101 ttgctgacgc accaagtgaa aaagtgtgtc cagagcggca tcaaagtgct gggactggca
    11161 gcgctcgata gcacagcaac accttgctat gaccgcgata cggcccaggc gctggttaat
    11221 gtcggcgcac aaatagccgc catgacaccg ggcgagctgg catcatggct tgcggagaat
    11281 cttcagtcat gaattcacta cgtccggaat tattagaact gacaccgcag gcactgacgg
    11341 cgttaagcaa tgccggtttt gttaagcgca gccttaagga actggaaaat ggcaacgtcc
    11401 cggaaatcag ccatgagaac agcgctttaa tcgccacctt cagtgacggt gtccgtaccc
    11461 agctggcgaa cggccaggca ctgaaagagg ctcagtgcac ctatggggcc agcggcatgt
    11521 gccgtcatcg cgtgatgctg gtgttaagtt atcaacgact ttgtgctacc gcccagccta
    11581 cgggaaaaga agaggaatgg gatccggcaa tctggctgga agaactggct acccttcccg
    11641 atgccacccg caaacgcgca caggcgctgg tcgctaaaag catcaccatt gagttgttct
    11701 gtgcgccggg tgaaattccc tctgcccgct taccgatgag cgatgtgcgt ttttattccc
    11761 gcagcagtat tcgtttcgcc cgttgtgatt gtattgaagg cacactttgc gaacatgtcg
    11821 tactggcagt acaggccttt gtccaggcaa aagcgcagca agcggaattg actcatttaa
    11881 tctggcagat gcgcagcgaa cacgtcacat catctgacga tccgtttgcc agcgaagaag
    11941 gcaaaacgtg tcgtcaatat gttcaacaac taagccaggc attatggctg agcggcatca
    12001 gccagccgct tattcactac gaggccgctt tcagccgcgc gcagcaagca gcggaacgct
    12061 gcagctggcg atgggtgagt gaatcgctac ggcaactgcg cgccagcgtt gatgccttcc
    12121 acgcccgcgc cagccactat catgccggag aatgcttacg tcagcttgcg gcattaaaca
    12181 gtcgattaaa ttgcgcacaa gagatggccc ggagcgacag tgttggtgaa gttcctcctg
    12241 tgccgtggcg cacggtcgtt ggctctggca ttgccggaga agcaaagctt gatcatctgc
    12301 ggctggtgtc tttaggtatg cgttgctggc aggatattga gcattatggt ttacgcatct
    12361 ggtttaccga tcccgacacc ggcagtattt tgcacctttc gcgcagttgg ccgcgaagtg
    12421 aacaggaaga ctcaccggca gctacgcgtc ggctgtttag ttttcaggct ggcgcactgg
    12481 cgggtgggca aattgtttca caagcagcaa aacgcagtgc cgatggcgag ctgctgttag
    12541 ctacccgcaa ccgcttaagc agcgttgtac cgctgtcgcc tgatgcctgg caaatgttga
    12601 gcgcgccgtt acgccagccg ggcattgtgg ctttgcggga atatttacgc cagcgtcccc
    12661 ccgcctgcat acggcctctt aatcaggtcg ataacttatt tattctgccg gtcgctgagt
    12721 gtatttcgct cggttgggat agcagccgcc agacgctgga tgcgcaggta atcagcggcg
    12781 aaggtgaaga taatctgctg acgttatcac tgccagcctc agccagcgcg ccttatgcca
    12841 ttgaacgcat ggcggcgctt ttgcaacaaa cagacgaccc cgtgtgtctg gtttctggct
    12901 ttgtcagttt tgttgatggg caattgacac tggaaccaca ggtaatgatg acaaagactc
    12961 gtgcctgggc gctggacgca gaaactgcgc ctgtggtggt atcactacct tctgccagcg
    13021 ttctgcctgt gccgtctact gctcatcagt tgctgatgcg ctgccaggcg ttacttattc
    13081 aactgctcca taacggctgg cgctatcagg aacagagcgc tattaatcag gcagagttgc
    13141 tggcgaatga cctcaccgcg gtgggttttt atcggctggc acatgtgttg gcacaatttc
    13201 gtaatacaga aagcgaggca cgggtagaag caatgaataa cggtgttttg ctttgcgaac
    13261 aattattccc catgcttcag caacaaggat gaaatagtgc tttttactaa gagttctact
    13321 ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc ctggtgagag
    13381 tcac
//
LOCUS       DAZSNM010000108        13248 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZSNM010000108 DAZSNM010000000
VERSION     DAZSNM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13248
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.108"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHW42_002553"
                     /pseudo
     CDS             complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHW42_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHW42_002554"
     CDS             complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHW42_002554"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR8944331.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2163..3197
                     /gene="rhaT"
                     /locus_tag="ACHW42_002555"
                     /pseudo
     CDS             2163..3197
                     /gene="rhaT"
                     /locus_tag="ACHW42_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACHW42_002556"
     CDS             complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACHW42_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR8944332.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACHW42_002557"
     CDS             complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACHW42_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR8944333.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5240..6709
                     /gene="rhaB"
                     /locus_tag="ACHW42_002558"
     CDS             5240..6709
                     /gene="rhaB"
                     /locus_tag="ACHW42_002558"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR8944334.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6706..7965
                     /gene="rhaA"
                     /locus_tag="ACHW42_002559"
     CDS             6706..7965
                     /gene="rhaA"
                     /locus_tag="ACHW42_002559"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR8944335.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8270..9031
                     /gene="rhaD"
                     /locus_tag="ACHW42_002560"
     CDS             8270..9031
                     /gene="rhaD"
                     /locus_tag="ACHW42_002560"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR8944336.1"
                     /translation="MKGWDERNGGNLTLRLDDADIALYHDNFHPQPRYIPLSQPMPLL
                     ANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTNEAVPTSELPAHFL
                     SHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVG
                     ILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSGPTLDEAFGLIDTAEKSAQVLV
                     KVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            9041..9355
                     /gene="rhaM"
                     /locus_tag="ACHW42_002561"
     CDS             9041..9355
                     /gene="rhaM"
                     /locus_tag="ACHW42_002561"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR8944337.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9656..10102
                     /locus_tag="ACHW42_002562"
     CDS             9656..10102
                     /locus_tag="ACHW42_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR8944338.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10113..11564
                     /locus_tag="ACHW42_002563"
     CDS             10113..11564
                     /locus_tag="ACHW42_002563"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR8944339.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11554..12624
                     /locus_tag="ACHW42_002564"
     CDS             11554..12624
                     /locus_tag="ACHW42_002564"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR8944340.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12624..>13248
                     /locus_tag="ACHW42_002565"
     CDS             12624..>13248
                     /locus_tag="ACHW42_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFR8944341.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3158 a         3458 c         3491 g         3141 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc cagcttcttc tttcgtaccg
      601 tactgctgca tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc
      661 gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc
      721 gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat
      781 ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac
      841 accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc
      901 cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc
      961 ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt
     1021 tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga
     1081 cattcattta atcaataaaa gtgatcaaaa tcacatcaat tgaaacgctg ttttattttt
     1141 ataggaccat tgtttttatt ttggaattga tcacaaaaaa accaccgccg ttggcgatgg
     1201 ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg caaatgatta
     1261 ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt
     1321 gatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag
     1381 gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg
     1441 gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga
     1501 accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa
     1561 gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt
     1621 ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc
     1681 tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg
     1741 caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg
     1801 gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga
     1861 tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc
     1921 gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat caatgccgta
     1981 cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact
     2041 taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag
     2101 cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga
     2161 agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag
     2221 cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag
     2281 tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg
     2341 atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg
     2401 gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt
     2461 cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa
     2521 ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg
     2581 gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc
     2641 gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga
     2701 tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg
     2761 aagccgctgc cgcacttggc gtcgatccac tgtatgttgc tctgccaagc tatgttgtca
     2821 tcatgggcgg cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga
     2881 aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt
     2941 tactctcggc actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc
     3001 acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg
     3061 tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc
     3121 cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca
     3181 tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc
     3241 cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg
     3301 aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga
     3361 cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa
     3421 cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc
     3481 tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg
     3541 aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag
     3601 ccatttcgtt agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct
     3661 gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc
     3721 taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa
     3781 taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa
     3841 tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat
     3901 taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt
     3961 gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga
     4021 gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg
     4081 caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc
     4141 tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta
     4201 ctgtcgctga atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagt
     4261 agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt
     4321 tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca
     4381 tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt
     4441 gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc
     4501 ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc
     4561 atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac
     4621 ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat
     4681 cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga
     4741 tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca
     4801 ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga
     4861 tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac
     4921 ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc
     4981 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
     5041 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
     5101 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
     5161 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
     5221 aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc
     5281 cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga
     5341 aatccatcgt tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga
     5401 tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat
     5461 cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca
     5521 gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc
     5581 acaacagcaa ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt
     5641 caatacgctt tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat
     5701 tgctcacgct ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg
     5761 ggaatatacc aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga
     5821 gtcgctactg gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg
     5881 taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc
     5941 cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta
     6001 tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga
     6061 cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct
     6121 gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga
     6181 tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc
     6241 caatgacgat cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg
     6301 ggaaacggcg caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag
     6361 tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt
     6421 ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc
     6481 cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat
     6541 cggcatcgag ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt
     6601 cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta
     6661 tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg
     6721 gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag
     6781 gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt
     6841 tccggttttg aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat
     6901 ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg
     6961 attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc
     7021 tcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat
     7081 cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt
     7141 acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc
     7201 cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg
     7261 atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg
     7321 gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt
     7381 gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac
     7441 atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg
     7501 actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtaccgca gttgctgctg
     7561 cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc
     7621 caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt
     7681 gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg
     7741 aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg
     7801 gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgcggcag
     7861 gcggtctggg aaatgtattg ccaacatcac gatacgccag caggtagcga atggctggag
     7921 agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg
     7981 gcacgagcgc cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc
     8041 ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa
     8101 caccgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc
     8161 cgcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac
     8221 ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctagc tgaaaggctg
     8281 ggacgagcgc aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta
     8341 tcacgacaac ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact
     8401 ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga
     8461 tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct
     8521 ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca
     8581 ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac
     8641 caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact
     8701 gtgggaaggc agcaccgaat gtctggtagt attcccggat ggcgttggca ttctgccgtg
     8761 gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc
     8821 gctggtgttg tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt
     8881 tggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg
     8941 cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt
     9001 tacaccacta gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt
     9061 catgcaggta aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc
     9121 ggaactggaa gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa
     9181 agcgcgtaat ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt
     9241 agccagtact gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa
     9301 cccagataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc
     9361 caatgtgccg gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat
     9421 attacatctc ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat
     9481 gaataaccca ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc
     9541 aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc
     9601 acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc
     9661 agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct
     9721 gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac accagttcct
     9781 gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg gtgtcgcagt
     9841 gccgcacggt aaaagcgcct gcgttaaaca accgttcgta ttattcgccc gcaaagcgca
     9901 ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct gcctcggcgt
     9961 gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc gcaaaattat
    10021 tcaccaggaa tttattcatc aactgcaaca gggcgatacc gaccaggtgc tggccttgtt
    10081 aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttacgt attgtcgcga
    10141 tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc ctggaacaga
    10201 aagcccgttc tctcggccat accataaaag tggaaactca agggtccagt ggcgttgaaa
    10261 accgcttatc cagcgaagag attgccgctg ccgattacgt cattcttgct accgggcgtg
    10321 gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag attgccatct
    10381 ccctggcgtt gaaaaatatc gaccagattt tcagcgaatt accgacaaac tcgcagcttt
    10441 ttgccgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc agcgtgatga
    10501 gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg
    10561 tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg
    10621 cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc tttatgatcc
    10681 ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc gctccggcgt
    10741 ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat
    10801 tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg ttccgcaaag
    10861 tgcgccttgg caaagcatta caaccgctgc tcggttcaat gctgatcccg ttcgttactc
    10921 tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc gacctcatgg
    10981 gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg gcagcgtttc
    11041 tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc gcctggttct
    11101 tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc ggcgttgtcg
    11161 cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc
    11221 gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac
    11281 cggctatacc ttacgctctg gcagctccgt tgccgatgat taccgccaat acgctggcgg
    11341 gcggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca cctggtttgg
    11401 gtatctttga cccgctgatc ggcctgatgt cgccggtagg ttcgttttat ctggtgctgg
    11461 cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg tggttacgcc
    11521 gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact gcaacagttg
    11581 tgcgaagcca gcgccgttag cggcgatgaa caggaagttc gcgacatcct gataaacacg
    11641 ctggaacctt gcgttaatga aatcaccttt gatggtctgg gcagctttgt tgcccgtaag
    11701 ggaaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc
    11761 acccacatcg acgagagcgg ttttctgcgt ttcaccacca ttggtggctg gtggaatcag
    11821 tcgatgctca accatcgggt aaccatacgc acacacaagg gagtgaaaat ccctggtgtg
    11881 attggtttcg tcgcgcctca tgcgttaacg gaaaagcaaa ggcaacagcc gctgtcattt
    11941 gatgagatgt tcattgatat tggcgcaaac agtcgcgatg aggtggaaaa acgcggtgtg
    12001 gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga agataaagtg
    12061 gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact attgcagacg
    12121 gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga agttgggcta
    12181 cgcggggcac aaacctcggc ggaacacatt aaaccggatg tagtgatcgt gctggatacc
    12241 gctgtcgcag gcgatgttcc gggcattgat aacattaaat acccgctgaa actgggccag
    12301 gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact ggtagcagcg
    12361 ttaaaaaact gtgccgcaca taacgattta ccgctgcaat tttccaccat gaaaaccgga
    12421 gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc
    12481 ctgccaaccc gctatctgca cgctaacagc ggcatgattt caaaagccga ttacgatgct
    12541 ctgctcacgt tgatacgggg ttttctgacg accttaaccg cggagaaagt caacgcgttt
    12601 agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc
    12661 tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt tcaggcagga
    12721 ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc cgcattttcg
    12781 ccagtggcag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc
    12841 tgcaaaagca cgataacgac gatcggctgc tggcgctgtt attactgaat actttcactc
    12901 cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtagca gagcgtctgc
    12961 cccagttaaa acagcgttat gaacgcactt gttgcctggc cagccgccct ggtttgggcc
    13021 atttcattga tgagacagaa gagaaactcg ttatcttgct ggcgaacttg ctgcgcaaag
    13081 atccgttttt aattccgctg gcgggcataa cacgagacaa ccttcagcat ttatccacgg
    13141 cctgcgacaa ccaacaccgc tggccgctca tacagggtga ttatctctcc agcctgattc
    13201 tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaat
//
LOCUS       DAZSNM010000109        13247 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZSNM010000109 DAZSNM010000000
VERSION     DAZSNM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13247
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.109"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..316
                     /locus_tag="ACHW42_002566"
     CDS             <1..316
                     /locus_tag="ACHW42_002566"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944342.1"
                     /translation="EIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYDWY
                     AEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNEIE
                     SD"
     gene            complement(382..614)
                     /locus_tag="ACHW42_002567"
                     /pseudo
     CDS             complement(382..614)
                     /locus_tag="ACHW42_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(724..1494)
                     /gene="yafV"
                     /locus_tag="ACHW42_002568"
     CDS             complement(724..1494)
                     /gene="yafV"
                     /locus_tag="ACHW42_002568"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR8944343.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            1675..2121
                     /gene="ivy"
                     /locus_tag="ACHW42_002569"
     CDS             1675..2121
                     /gene="ivy"
                     /locus_tag="ACHW42_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR8944344.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2164..4608)
                     /gene="fadE"
                     /locus_tag="ACHW42_002570"
     CDS             complement(2164..4608)
                     /gene="fadE"
                     /locus_tag="ACHW42_002570"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR8944345.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4848..5426
                     /gene="lpcA"
                     /locus_tag="ACHW42_002571"
     CDS             4848..5426
                     /gene="lpcA"
                     /locus_tag="ACHW42_002571"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR8944346.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5632..6399
                     /locus_tag="ACHW42_002572"
     CDS             5632..6399
                     /locus_tag="ACHW42_002572"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR8944347.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6370..7110)
                     /gene="dpaA"
                     /locus_tag="ACHW42_002573"
     CDS             complement(6370..7110)
                     /gene="dpaA"
                     /locus_tag="ACHW42_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR8944348.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            7494..8015
                     /locus_tag="ACHW42_002574"
                     /pseudo
     CDS             7494..8015
                     /locus_tag="ACHW42_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            8191..8688
                     /gene="rayT"
                     /locus_tag="ACHW42_002575"
     CDS             8191..8688
                     /gene="rayT"
                     /locus_tag="ACHW42_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR8944349.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(8905..10617)
                     /locus_tag="ACHW42_002576"
                     /pseudo
     CDS             complement(8905..10617)
                     /locus_tag="ACHW42_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            10589..11374
                     /gene="lafU"
                     /locus_tag="ACHW42_002577"
     CDS             10589..11374
                     /gene="lafU"
                     /locus_tag="ACHW42_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR8944350.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            11445..12500
                     /gene="dinB"
                     /locus_tag="ACHW42_002578"
     CDS             11445..12500
                     /gene="dinB"
                     /locus_tag="ACHW42_002578"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR8944351.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTYGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            12552..12845
                     /gene="yafN"
                     /locus_tag="ACHW42_002579"
     CDS             12552..12845
                     /gene="yafN"
                     /locus_tag="ACHW42_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFR8944352.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            12848..13246
                     /gene="yafO"
                     /locus_tag="ACHW42_002580"
     CDS             12848..13246
                     /gene="yafO"
                     /locus_tag="ACHW42_002580"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFR8944353.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
BASE COUNT         3205 a         3461 c         3416 g         3165 t
ORIGIN      
        1 cgaaattaga ataagaattg ctggtaatac ggctgcgcct tgtttccatg tgctaaaaaa
       61 taagggatat aaagtaacaa tggaaactca tgcattatct gacgattgtt tagattgtgt
      121 atatgattgg tatgcggaaa aaaatggaca attttttagt gcaacgagtc cagaagagtt
      181 acttggatta gttgcaatgt gggaagttcg tggtaatgac tggagagcta atttgaatga
      241 gataaatact tatcaaaaag ccatagaaga atccccgttt tttgataaaa acggcaatga
      301 aatagagtct gactaaattt tattgatata gcaacgataa taaccggaaa tttcttccag
      361 atctttccgg ctttttatta tttaactttc gttcagcaca ctaaacatcc cgtctcgcat
      421 ccccttaact ggctgtttaa cctgataaag ttccctgcgc agttcctgta cttcgttgtt
      481 atcccaagca ggatcaaaca tccctccgag atcttcacgg taacgcccgt gccgatctca
      541 aatcccgctt ttctcagcca tttggcgctg attttgatca ccggcgtgtc gctgcggttg
      601 ggcagataac ccacggtaca gcgacgctgt gttttggaaa tggtgacttc tggcgtaata
      661 ttgtgtttaa gttaggggct ttcgtaatct agcccagccc ccggccctaa acacaacccc
      721 cactcaccac aacctaaact catccgcatc ccgccatgcc ggaaactttt ctctatattc
      781 ccgcaatgcc accatcgaca gctccgcatc aatacgcgtt gccttatgcg cgtcggcggt
      841 agcgataatc tcgccttgcg gattaatcac ccggctgtca ccgcgataat ggcagccgtt
      901 gccatcgctg ccgacgcgat tgcatcccgc cacatacgcc tgattctcaa tcgcgcgggc
      961 cgtcagcaat gcctgccagt gcagagagcg cggagctggc cagttggcga cgtacagggc
     1021 gaggtcataa tcgttgagat tgcgcgacca cactggaaaa cgtaagtcgt agcacaccag
     1081 cggcaaaata cgccagccgc gccattccac aatcactcgc gcattgcccg ctttataatg
     1141 cagatgctca tctgccatgc ggaacagatg acgcttatca taaaaatgta ccgtgccgcc
     1201 cggctcaacc agcaaaaagc ggttaaccga accagactcc gtttgtaatg caacactgcc
     1261 tgcaatcagc gcattgcact gctgcgcctt agctgtcatc cagttcacta cgtcatcttg
     1321 tgcaagcgac gaagctgccg cttccatggc aaagccgctg gtaaacatct ccggtagaac
     1381 gatcacatcg cgcccggtaa taccttccag ttgacgatca aaatggcgca ggttggcagg
     1441 accatccacc cacaccaacg gttgctgcaa aagcgtaatc ttcaaactag gcacggtgta
     1501 caactccttt atgcgaaggg ttttataact ttaacacctt atcaggcagt tgccttagcg
     1561 cagaataaat tgataacaaa tgctgatatt ggaaatatct gatttgcaaa ttatcgtgtt
     1621 atcgctaggc tttaggaggt taataacatg ggcaggataa gctcgggagg aatgatgttt
     1681 aaggcaataa cgacagtcgc cgcactggtc atcgccacca gtgcaatggc gcaggatgat
     1741 ttaaccatta gcagccttgc aaagggcgaa accaccaaag ctgcgtttaa tcagatggtg
     1801 caagggcata agctacctgc atgggtgatg aaaggcggta cttatactcc cgcacaaacc
     1861 gtaacgttgg gagatgagac gtatcaggtg atgagcgcgt gcaaaccgca tgactgtggc
     1921 tcgcaacgta tcgctgtgat gtggtccgag aaatctaatc agatgacggg gctgttctcg
     1981 gctattgatg agaaaacgtc gcaagagaaa ctcacctggc tgaatgtgaa cgatgcgctt
     2041 tcgattgatg gtaaaacggt gctgttcgcg gcgttgaccg gcagcctgga aaaccatccg
     2101 gatggcttta attttaaata attagcggat aaagaaacgg agcctttcgg ctccgttatt
     2161 caattacgcg gcttcaactt tccgcacttt ctccggcaac tttaccggct tcgtcgccag
     2221 ctcttccgga tcaaagtcat caacgttaat actgcgcaga cggctttctt ccgctttcac
     2281 cagaatagcg gcttcatctt tatcaatcag ccccttcgtc agcgcgttgt gcgccagttc
     2341 atccagacgg gtaaatggca gatttttacc cagctcttta cagatccgct gatgaatcgg
     2401 gtcggcggca atcacatcca ccaacgcctc ttccagtaaa ccaaccgggt tatgctcgct
     2461 tggcgtcagg tactgaccgc gaccaatacg tgaacgagtg gcgttcggca cttgtaaaat
     2521 cttcgccatt ttatgatcca gcttgtcaga cggtgccaga taatgacgtc cggtcgggaa
     2581 gatcaccaca ttcagcagcc cggcaaccac gcggttcggg aagttttgca gtaaattgtc
     2641 cattgcctgt tctgcctgat acagcgcatc ctgtacgccc cagtgcacca gcggcagatc
     2701 ggcttcatta cggccttcgt cgtcataacg cttcagaacg gcagaggcga ggtaaagctg
     2761 gcttaaaata tcccccagac gtgccgagat acgctcacga cgtttcaggc tgccgcccag
     2821 cagtgccatc gagacatcag aaagcagggc gaggttggcg ctcaggcggt tcaggtgctg
     2881 atagtagcgt ttagtggcat cgccggttgg cgtgctgctg gttaaaccgc gcgtcaggcc
     2941 cagccagaag ctgcgaactt tgttgctacc gacgtgaccg atatgtttga acaacagttt
     3001 atcgaacgcg ttgacgtcat tgttcttcgc cgcttccatc tcttccagca cgtacggatg
     3061 gcaacgaatc gctccttgtc cgaagatcat catgctgcgg gtcagaatgt tagccccttc
     3121 aacggtgatg gcaatcggtg cgccctggta agcacgcgcc aggaagttgc tttgcccgag
     3181 cataatgcct ttaccgccgg taatatccat cgcatcaata atcgactgct gcccgcggtg
     3241 ggtacagtga tacttaacga tagccgacag cacggcaggt ttttcgccga gcataatgcc
     3301 gtaggtaatc agcgatgccg cagcatccat tacataagcg ttcccggcaa tacgcgccag
     3361 cggctcttca atcccttcca tcttaccgat agagattttg aactgacggc gaatgtgaga
     3421 atacgcgccc gttgccagcg ctaccgattt cacgccgccg gttgagttgg aaggcagggt
     3481 gatgccgcgg cctaccgaga ggcactccac cagcatccgc cagccttgcc cggccatttt
     3541 cggcccgccg atgatgtaat cgatcggcac gaagacatct ttaccgcgcg tcggtccgtt
     3601 ctggaacggt acgttcagcg ggaagtggcg acgaccaatt tccacgcccg gcgtggtggt
     3661 tgggatcagc gcacaggtaa tgcctaaatc ttctgcaccg ccgagtaatt tttccgggtc
     3721 ggagagttta aacgccagcc caagcacggt cgcaatcggt gccagcgtaa tgtagcgttt
     3781 gttccaggtc agacgcatcc ccagcacctg ctggccctgc cattcgccca tgcagacaat
     3841 cccggtgtcc ggaatcgcgc ccgcatcgga acccgcttcc gggctggtca gtgcaaagca
     3901 ggggatctcc tgaccacgcg ccagacgcgg cagatagtga tctttctgct cgtcagtgcc
     3961 gtaatgttgc aacagttcgc ccgggcctaa tgagtttggc acgccgacgg taatcgccag
     4021 gatcccgctc acgccagaga gtttttgcag cacacgagac tgggcataag ctgagaactc
     4081 cagtccgccg tactcttttt tgataatcat cgcgaagaag cgatgctctt taaggtacgc
     4141 ccacaactcc ggtggcagat ccgccagctc atgagtaatc tggaaatcat tcgccatccg
     4201 gcaggcttct tctaccgggc cgtcgagaaa cgcttgctct tcagcggtca ggcgcggctg
     4261 cggatagtta tgcagctttt tccagtccgg cttgccctgg aacaagtcgc cctcccacca
     4321 ggtggtgccc gcatcaatcg cttctttctc agtgcgcgac atcggcggca tcaccttacg
     4381 gaaaccgcga aataccggcg cggaaatcat cgacttacgc ataggcgcaa agttaaatgg
     4441 cacgaggata atggtcagag gcaccagtac ccacgccgac cacagaccag caacgccgag
     4501 ggcggctgtc caggcgagca aaatcagact gctgataaat aagctcacgc ggtgatagaa
     4561 caacgcgccg agcaggacaa ccgtagcgag aatactcaaa atcatcataa cgaaaagccc
     4621 cttacttgta ggaggtctga ccacttgtga tgatatggtt gtagtggatg taaaaacatt
     4681 tagcaatgtg tttacaatat aattacaaca aagctcacat tgttgctgtt tttatccgca
     4741 cttcaggtca aaaagtcctg gtcatagcac ctgcccgtac ttctcgcttt tggcggtatc
     4801 cggtacactg cattttgtct attacattta tgctgaagga tatcctcatg taccaggatc
     4861 ttattcgtaa cgaactgaac gaagcggcgg aaacgctggc taacttttta aaagatgacg
     4921 ccaatattca cgccattcag cgcgcggcgg tcctgttagc agacagcttt aaagccggtg
     4981 gcaaagtgct ttcctgcggc aacggcggtt cccattgcga cgctatgcac tttgccgaag
     5041 agttgaccgg tcgctaccgt gaaaaccgtc cgggctaccc ggcgattgct atttctgacg
     5101 ttagtcatat ttcctgcgtc ggtaatgatt tcggtttcaa tgatattttc tcccgctacg
     5161 ttgaagcggt aggtcgcgaa ggcgatgtac tgctggggat ctccacctcc ggtaactctg
     5221 caaacgtgat caaagcgatc gcagcggcgc gtgagaaggg gatgaaagtg atcaccctga
     5281 ccggtaaaga cggcggtaaa atggctggca cggcggatat cgaaattcgc gttccgcact
     5341 ttggttatgc cgaccgcatt caggagattc acattaaagt gatccatatc ctgatccagt
     5401 taattgaaaa agagatggtt aagtaagtct ggcgtaggcc ggataaggcg tttacgccgc
     5461 atccggcact tgtgcgctga tgcctgatgc gatgctgacg cgtcttatca tgcctacaaa
     5521 tctgtacccg aaccgtaggc cgaataattc gtttacgccg catccgacct gaaaattctt
     5581 aaatcaatct tcgccggggt ccatgcgctc ccgctgttgt ggaggttacc catgtgcgaa
     5641 ttgctcggga tgagcgccaa cgtccctacc gatatctgct ttagtttcac cggacttgta
     5701 cagcgtggtg gtggaaccgg gccacataaa gatggctggg gcattacctt ttacgaaggt
     5761 aaaggctgtc gcacatttaa agatccacaa cccagcttta attcccccat cgccaaactt
     5821 gtccaggact acccgataaa atcctgttcg gtggtggctc atattcgcca ggctaatcgg
     5881 ggcgaggtgg cgctggaaaa tactcaccca tttacccgcg agttatgggg gcgtaactgg
     5941 acttatgccc ataacggaca actgacgggc tacaaatcac tggaaaccgg caacttccgc
     6001 ccggtcggtg aaaccgacag cgaaaaagcc ttttgctggc tgctgcataa attaacgcag
     6061 cgttacccgc gcacgccggg caacatggcg gcagtgttta aatatatcgc ctcactggcg
     6121 gatgaactgc gggcgaaggg cgttttcaac atgctgcttt cggacgggcg ctatgtaatg
     6181 gcgtattgct cgactaattt acactggatc acccgccgcg cgccgtttgg cgtggcaacg
     6241 ttgctggatc aggatgtgga aatcgacttc agctcgcaga ccacaccgaa tgatgtggtc
     6301 acggtgattg cgacacagcc gctgacgggc aatgaaacct ggcaaaaaat tatgccaggc
     6361 gaatggcgct tattttgcct cggggagcgt gtagtttgat gccagttgtg gctgcaccac
     6421 ttcgtggctt aacggcttgc tgaccacgta acggccattg gccacagaaa cggtcggtgg
     6481 cttacgggtt tgctcaaagt agtcgtagcc cggcttcagc tgctcccaga agtccttaaa
     6541 gttggaatat ttatggcgct tcatattggc gtcggtcatg cggaatgggt aaatactcac
     6601 ctgcacgctc ggctgaccaa acaccagcgc accagtaacg aactggaaga tctcatcaat
     6661 accctgattg gtcattgcgt agcagccgat ggatacacaa tcgccgtgga tcatcaggta
     6721 tttcccttca taaccatgcg cacggtcata agcattgggg aatccaatat taatcgcttt
     6781 gtagtaacgg ctgtctggtt ttaactgatt acgctggacg ctataaaacc cttccgggct
     6841 tttgaaatcg ccctgacgct gttttggccc taagccgccg gaatatttac agattttata
     6901 actgtcgagc agttgatatt gctcgcccat tttgacgtaa agatcgagcg tacgttcttc
     6961 cttgaagatc tggatgtaga caggggatcc catcaactgc tgcttatact ctttgctcac
     7021 tggcgtggtc gagctactgc tgcccagcaa accggcaaac gaaacgcacg ggatcaacaa
     7081 catcgcaaga attaatgcga ttttacgcat actgcttatt ccttgataaa acggttacac
     7141 acgccaggac ggcaaaatgg atcccaaatc ggaatagtct ggatttggaa ggctcacatt
     7201 atcaccaaaa gagttttacg caagcctgtc ggcgctgggt ttacaaattt agcacagggg
     7261 cagttttcaa tagtccgaaa agcgcatgta tcatttattt cctaatatta tgaaatctca
     7321 atagtaatat gcaaataagc aattaataat cccgtttact cctccggtct tttaaaattc
     7381 tcaccttcat tcagccggtt tatgtctttc atgtcttcgt tcttgctcgg cagatttttg
     7441 catccaggcg tgttttcgtt atgcgttttg ctccctttac tcgccagcgc caccacaacc
     7501 gggagcagat aaaacagcat ccagaatggt ttcctgcgcc gctcaaggcg agtgacagac
     7561 gctggcaggc gctggcggaa aacaaccact ttttaagcag cgatcacctg cataacatca
     7621 ccgaagtggc gattcaccgc ctggagcagc agcttggcaa gccttacgtc tggggcggca
     7681 cgcggcccga tcaaggcttc gattgtagtg ggttagtttt ttatgcctac aacaagatcc
     7741 ttgaggcgaa actcccgcgc acggccaatg agatgtacca ctaccgtcgg gcaacgattg
     7801 tggcgaataa cgacctgcgc cggggagatt tgctattttt ccatatccac agccgcgaga
     7861 ttgccgacca tatgggggtg tatctgggcg acggtcagtt tattgagtca ctgcgcaccg
     7921 gggaaaccat tcgggtaagc cggttagccg aacctttctg gcaggaccat tttttaggcg
     7981 cgcgcaggat tttgacggaa gagatgattt tgtaggccgg ataaggcgtt tacgccgcat
     8041 ccggcggttg tgcacggatg cctgatgcga cgctgacgcg tcttatcatg cctacaaatc
     8101 tgtgcgtttt tgcgagccgc tttcccgata taacacagcg tcattgtcgg cctttccttt
     8161 tcctcttacc gttgagaaaa aggagtcgct atgtcggaat atcgtcgtta ttacatcaag
     8221 gggggaacat ggtttttcac ggtaaattta cgcaatcgtc gaagccatct tctgaccacc
     8281 cagttccaga cgctccgtaa cgccattatt aacgttaagc gagacagacc ttttgaaatt
     8341 aacgcctggg tcgttttgcc ggagcatatg cattgcatat ggacattacc cgaaagcgat
     8401 gatgattttt cctctcgctg gcgggagatt aaaaagcagt tcacccatgc ttgcggattg
     8461 aaaaatatct ggcaaccgcg tttttgggag catgccattc gcaatacaaa agattatcgc
     8521 catcatgttg attatattta tataaatcca gagaaacatg gctgggtaaa gcaggtgagt
     8581 gattggccat tctcaacgtt ccatcgcgat gtcgcgcgag ggttatatcc catcgattgg
     8641 gcgggagaca taacggattt aagtgccggg gagcgtatca ttttataact gtgcgctggt
     8701 gccggatgcg cttacgctta tccggcctac aaatcgtgct ttaacggtag gccggataag
     8761 gcgttcacgc cgcatccggc agttatgcgc agatgcctga tgcgacgctg gcgcgtctta
     8821 tcatgcctaa aaacttgtgc ctgaacggta ggccggataa ggcgtttacg ccgcatccgg
     8881 caattgtgca ccgatgccgg attcttacat caacgccccc ataatcttca actccagctc
     8941 atccggcact tcgttatacg acagcacatg cagccccggc gcaaacaacc ttgcataacg
     9001 cggcagcaaa gggcgtagct gcggcggcac cagcagcacc ggatctttcc ccgccgcttt
     9061 catctgctct ttcacctgtg gcatcgtgct ctggaactgg ttgagcatat tcgggtcaac
     9121 cggcacgctg tcgagcatca ctttgccggc ctgttgcgcc tgattcacta cgttggtcag
     9181 cagattttcc agctcattat tcagcgtata caccgtcagc tcctgcttgc gaacgaacgg
     9241 atgagtaatg ctgcgccgca gcgccaggcg cacatcggcc gccagcagaa tatgatcttt
     9301 agtcaccgtg ctactggcga ccagcacggt ggcgatggtg acgatatcgc gcagggaaac
     9361 gccttcggtc agcagcgccc ggtacacttt cagcaattgg ctgtaattga gcgccgcgct
     9421 taaatcctcc gccaggcgcg gtgccatcga cgacaaacgg ttatgcaact gcgtgatgtc
     9481 atcatagtta aacaaatcag gaatatagct gcgcacaatc ttgttcacat gggtggcaat
     9541 cacactggcg ctgtcgatca cctgataccc catattcaga gccttcgctt tctgcgcagg
     9601 ctggatccag gtgaccggta tcccgtacgc cgggtcgttc cccagcacgc cgtcaatctc
     9661 gccgtaggtt tcgctggagg gcagcgccat cagtttatcc gccggaatat ccgcttcatc
     9721 agcctcaatg ccgttgatga aaatagcgta ctggctgggc ttgaggcgga agttttcccg
     9781 aatgcggatc tccggcagca gcacgccgtt accgtcggaa atcacctgcc gaacgccgcg
     9841 aatccgctgg gtgagcgggt tgccctgcgc tttgtccacc agcgccacca gcttgtagcc
     9901 gaggcttaag ctgatgggct cgatcagcgg aatagtttcc caactgacct gttgttcgct
     9961 ggtttcagtg atggtgcggg tcagcgtttc gaggcttttc tcttccgcct ccgccgcctg
    10021 cgggcgtttg ctcatccgcc agccggtaaa gccaagcagg gtgctgaaca gcaaaaacgg
    10081 cagatgcggc atccccggca ccaccgccag cacgaacatg atcccggtgg cggtgtagag
    10141 caccgacggg cttgccagca actggtggcg cacgtcatgg gcgatatcgc cgttgtcgct
    10201 gacgcgggtg acgataatcg ccgccgcggt ggagagcagc agggaaggga tctgcgccac
    10261 caggccgtcg ccgatggtca tcagcacgta ttgctggaag gcggcgtcgg cgctcaggtt
    10321 gtatttgaag atgccgatac agacgccgcc gctcaggttg atcgccagaa tcatcatccc
    10381 ggcgatggcg tctccgcgta caaacttcga tgccccgtcc atcgcgccgt agaaatcggc
    10441 ctcgctggca acatctttac gccgggtttg cgcctgcgcc tggttgatca atccggcgtt
    10501 aagatcggcg tcaatcgcca tctgtttgcc gggcatcgcg tccagggtaa agcgggcaga
    10561 aacctcggaa atacgctcgg cctctttggt gacgacgata aagttgattg tcaacagcgt
    10621 cagtaaatcc gagcgtgaaa gcattatcgc cgcgctgcac gggcagtcga tttttaacgg
    10681 tggtgggctg tcgccgttaa ataaaatcag cccgtctcat ccgccaaaac ctgccaccgt
    10741 ggcggtgccg gaagagacgg agaaaaaagc gcgtgatgtg aatgagaaaa cggcgctgct
    10801 gaagaagaaa tccgccaccg agcttggcga gttggcaacc agtatcaaca ccattgcccg
    10861 tgatgcgcat atggaagcga atctggagat ggagattgtt ccccagggat tacgcgtgct
    10921 gattaaagac gaccagaatc gcaatatgtt cgaacgcggc agcgcgcaga ttatgccgtt
    10981 ctttaaaact ttgctggtgg agctcgcgcc agtgttcgac tcgctctata acaaaattat
    11041 tattaccggg cataccgatg cgatggccta caaaaacaat atctacaaca actggaacct
    11101 ttcgggcgat cgcgcgcttt cagctcgtcg ggtgctggaa gaggccggaa tgccggaaga
    11161 taaagtgatg caggtaagcg caatggcgga ccagatgctg ctggacgcga agaatccgca
    11221 aagcgcgggc aatcggcgta ttgagattat ggtgctgacc aaaagtgcgt ccgatacgct
    11281 gtatcaatac tttggtcagc atggggataa agtggtgcag ccgctggtgc aaaagctgga
    11341 taagcagcag gtgctttcgc agcgaatgcg ctaaatgctg aatctttacg catttctcaa
    11401 aacctgaaat cactgtatac tttaccagtg ttgagaggtg agcaatgcgt aaaatcattc
    11461 atgtggatat ggactgcttt ttcgcagcgg tggagatgcg cgacaatccc gctctgcgcg
    11521 atatccctat tgctattggc ggcagccgcg aacgtcgggg ggtgatcagc accgccaatt
    11581 atcccgcgcg taaatttggc gtacgtagcg ctatgcctac tgggatggcg ctcaaattat
    11641 gcccacatct caccttgctt ccggggcgct ttgacgccta caaagaagcc tcaaatcata
    11701 tccgtgaaat cttctcgcgc tacacctcgc gcattgaacc gttgtcactg gatgaggctt
    11761 atctcgatgt caccgatagc gtccattgcc acggttctgc gaccctcatc gcccaggaaa
    11821 tccgccagac gatcttcaac gagctgcaac tgacggcatc tgcgggcgtg gcaccagtaa
    11881 agtttctcgc caaaatcgcc tccgacatga ataaacccaa cggtcagttt gtgattacgc
    11941 cggcagaagt tccggcattt ttacaaacct taccactgga aaaaatcccc ggcgtcggca
    12001 aagtctcggc ggcaaaactg gaagcgatgg ggctgcggac ctacggtgat gtacaaaagt
    12061 gtgatctggt gattctgctt aaacgctttg gcaaatttgg ccgcattttg tgggagcgta
    12121 gtcaggggat tgacgagcgc gacgttaaca gcgaacggtt acgaaaatcc gtcggcgtgg
    12181 aacgcacgat ggcggaagat attcatcact ggtctgaatg tgaagcgatt atcgagcggc
    12241 tgtatccgga acttgaacgc cgtctggcaa aggtaaaacc tgatttactg attgctcgcc
    12301 agggggtgaa attaaagttc gacgattttc agcagaccac ccaggagcac gtctggccgc
    12361 ggctgaataa agctgactta atcgccaccg cgcgtaaaac ctgggatgaa cgccgcggcg
    12421 ggcgcggtgt gcgtctggtg gggctgcatg tgacgttgct tgacccgcaa atggaaagac
    12481 aactggtgct gggattatga tgtatactat tatgtatatt ctggtgtgca ttattatgag
    12541 ggtatcactg tatgcatcga attctcgctg aaaaatcggt caatatcact gagttacgta
    12601 aaaacccagc taaatacttt attgatcaac cagttgcggt tctttctaat aatcgccccg
    12661 caggatatct cttaagtgcc agcgcattcg aagcgttaat ggacatgctt gctgaacaag
    12721 aggagaaaaa gcccataaag gcgcgctttc gtccaagtgc tgcaagatta gaggaaatta
    12781 cacgccgcgc taaacaatat cttaatgata tgacggatga tgatttcaat gactttaagg
    12841 aataaggatg cgggtattca aaacaaaact tattcgcctg caacttacag cagaggaact
    12901 tgatgcgtta acggcggatt ttatttccta taagcgtgac ggtgttttgc cagatatatt
    12961 tggtcgcgat gcactctacg acgactcgtt tacctggcca ttaatcaaat ttgagcgagt
    13021 tgctcatatt catctggcaa atgtgaataa tccatttccg ccacagttgc gccaattcag
    13081 cagaacgaat gacgaagcgc atttggtata ttgtcagggt gcgtttgatg agcaagcatg
    13141 gttgctcatt gccattctga agcctgaacc tcataaactg gctcgagata acaaccaaat
    13201 gcataaaatt ggaaaaatgg cagaagcgtt tcgcatgcgt ttttgaa
//
LOCUS       DAZSNM010000110        13098 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZSNM010000110 DAZSNM010000000
VERSION     DAZSNM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.110"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHW42_002581"
     CDS             complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHW42_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR8944354.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="ACHW42_002582"
     CDS             1131..1931
                     /locus_tag="ACHW42_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR8944355.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="ACHW42_002583"
     CDS             2009..2779
                     /locus_tag="ACHW42_002583"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR8944356.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHW42_002584"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHW42_002584"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR8944357.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACHW42_002585"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACHW42_002585"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR8944358.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="ACHW42_002586"
     CDS             5034..5756
                     /locus_tag="ACHW42_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR8944359.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACHW42_002587"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACHW42_002587"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR8944360.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACHW42_002588"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACHW42_002588"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR8944361.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="ACHW42_002589"
     tRNA            7149..7225
                     /locus_tag="ACHW42_002589"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="ACHW42_002590"
     CDS             complement(7356..8402)
                     /locus_tag="ACHW42_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFR8944362.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACHW42_002591"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACHW42_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACHW42_002592"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACHW42_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR8944363.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACHW42_002593"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACHW42_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR8944364.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="ACHW42_002594"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="ACHW42_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
     gene            complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACHW42_002595"
                     /pseudo
     CDS             complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACHW42_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137593242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3007 a         3392 c         3484 g         3215 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc tagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatttggttt cattgtgt
//
LOCUS       DAZSNM010000111        13066 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZSNM010000111 DAZSNM010000000
VERSION     DAZSNM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13066
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.111"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            248..631
                     /gene="secE"
                     /locus_tag="ACHW42_002596"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="ACHW42_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR8944365.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="ACHW42_002597"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="ACHW42_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR8944366.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="ACHW42_002598"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="ACHW42_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR8944367.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="ACHW42_002599"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="ACHW42_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR8944368.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="ACHW42_002600"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="ACHW42_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR8944369.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="ACHW42_002601"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="ACHW42_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR8944370.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="ACHW42_002602"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="ACHW42_002602"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR8944371.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="ACHW42_002603"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="ACHW42_002603"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR8944372.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..>13066)
                     /gene="thiH"
                     /locus_tag="ACHW42_002604"
     CDS             complement(12705..>13066)
                     /gene="thiH"
                     /locus_tag="ACHW42_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="in Escherichia coli this enzyme functions in
                     thiamine biosynthesis along with thiFSGI and iscS; with
                     ThiFSG catalyzes the formation of thiazole phosphate from
                     tyrosine, cysteine and 1-deoxy-D-xylulose-5-phosphate;
                     forms a complex with ThiG; contains an iron-sulfur center;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR8944373.1"
                     /translation="FPRLRPCTGGIEPASIMDERQLVQTICAFRLLAPEIELSLSTRE
                     SPWFRDRVIPLAINNVSAFSKTQPGGYADNHPELEQFSPHDDRRPEAVAAVLTAQGLQ
                     PVWKDWDSYLGRASQRL"
BASE COUNT         3246 a         3251 c         3562 g         3007 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgattg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgccgcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaagg
//
LOCUS       DAZSNM010000112        13029 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZSNM010000112 DAZSNM010000000
VERSION     DAZSNM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13029
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.112"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(54..1115)
                     /locus_tag="ACHW42_002605"
     CDS             complement(54..1115)
                     /locus_tag="ACHW42_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR8944374.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1328..2791
                     /gene="bepA"
                     /locus_tag="ACHW42_002606"
     CDS             1328..2791
                     /gene="bepA"
                     /locus_tag="ACHW42_002606"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR8944375.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            2812..3171
                     /gene="arsC"
                     /locus_tag="ACHW42_002607"
     CDS             2812..3171
                     /gene="arsC"
                     /locus_tag="ACHW42_002607"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR8944376.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3304..4005)
                     /locus_tag="ACHW42_002608"
     CDS             complement(3304..4005)
                     /locus_tag="ACHW42_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR8944377.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4100..5389)
                     /gene="uraA"
                     /locus_tag="ACHW42_002609"
     CDS             complement(4100..5389)
                     /gene="uraA"
                     /locus_tag="ACHW42_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR8944378.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5475..6101)
                     /gene="upp"
                     /locus_tag="ACHW42_002610"
     CDS             complement(5475..6101)
                     /gene="upp"
                     /locus_tag="ACHW42_002610"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR8944379.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6425..7462
                     /gene="purM"
                     /locus_tag="ACHW42_002611"
     CDS             6425..7462
                     /gene="purM"
                     /locus_tag="ACHW42_002611"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR8944380.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7462..8100
                     /gene="purN"
                     /locus_tag="ACHW42_002612"
     CDS             7462..8100
                     /gene="purN"
                     /locus_tag="ACHW42_002612"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR8944381.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8272..10338
                     /gene="ppk1"
                     /locus_tag="ACHW42_002613"
     CDS             8272..10338
                     /gene="ppk1"
                     /locus_tag="ACHW42_002613"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR8944382.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10343..11884
                     /gene="ppx"
                     /locus_tag="ACHW42_002614"
     CDS             10343..11884
                     /gene="ppx"
                     /locus_tag="ACHW42_002614"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR8944383.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(11923..>13029)
                     /gene="pdeF"
                     /locus_tag="ACHW42_002615"
     CDS             complement(11923..>13029)
                     /gene="pdeF"
                     /locus_tag="ACHW42_002615"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR8944384.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         3370 a         3381 c         3285 g         2993 t
ORIGIN      
        1 acgtcaggaa tcctgaaaaa tcagcccggc gaaacagttc gtcgggctga agattattct
       61 tgcgcgattt gcccatcggg ccaggcgtgg atcacggctt tgatcagcgt cgccaatgga
      121 atggcgaaga atacgcccca gaatccccac aaaccaccga agatcaccac cgataaaata
      181 atcaccagcg gatgcaggtt aaccgcttcg gagaacaaca ccggtactag caggttgccg
      241 tccagcgcct gaataatcag atacaccgcg aagcagctcc agaattcagt acctgcgcca
      301 aactggaata gcgccacgcc aaccaccgga atggtcacca caaatgcgcc aatgtacgga
      361 ataagaaccg agaagccgac cagcactgcc agcagcagcg aatagttcag cccgaagagc
      421 aagaacccca gccaggtggc gatccccacc acgatcatct ccagcacttt gccgcggata
      481 tagttggtga tttgttgatt catctccttc cacacctgtc ctgccagccc acggttacgc
      541 ggcagcaccc ggcgaacggc gttcagcatc tgctctttgt ctttcagcag gaagaagacc
      601 atcaatggca ccagcaccag atagacggct atggtcagca aaccgaccag cgaggcgagg
      661 gaaattttca ccaccgaatc gcccatggtc agcatccgac tgcgcatatt ttcggccatt
      721 gcatcaataa tgcccgcgtc cattaacacc ggatagcggc gcggcaacgt ggcggcaaag
      781 tcagaaagct tattgagcat ccccggcata tcgcggatta agtagatgcc ctgttgccag
      841 gcgataggca gtaccacgaa cgccatcagt agcaatatac cgacaaaaac caccaataca
      901 atcgacgtcg cccagcggcg ggagcagcca atagattgca ggcgcacggt tggccattcc
      961 agcaaataag ccagcacaat agccaccagc aacggagcaa gcaggccact aaagaaaaag
     1021 ataatgccaa atccggcaac taaaataacc agcaaggcaa tcgcttccgg gtcgctaaaa
     1081 cggcggcgat accattgcat caacatttcg agcataaaga ccttccctga acctcaagag
     1141 cgggattgcg atccgcaatt gtatcgaaat gtcacaaaaa agacttcgct ttttatgacg
     1201 gattcaggaa actgaaaagt catttgagtg ggctaatctt cgccgttaca ctcaaaggcg
     1261 gcgaggtggg aacgatattt cacagtatcg gtcaaatgac tatcttcaga aatacaggat
     1321 agaggttatg ttcaggcagt tgaaaaaaaa cctggttgca accctcattg ctgctatgac
     1381 cattggtcag gtagccccgg cgtttgccga cagcgcagac accttgccgg atatgggaac
     1441 ctccgcagga agcacgcttt ccattggtca ggaaatgcag atgggcgact attatgtccg
     1501 ccagctacgc ggcagcgcgc cgttaattaa tgacccgctg ttaacgcaat atattaattc
     1561 gctggggatg cgtctggttt cgcatgccaa ttcggttaag acaccgtttc atttttttct
     1621 gatcaacaac gacgaaatta acgcctttgc tttctttggc ggcaacgtgg tgctgcactc
     1681 tgccctgttc cgttattccg ataacgaaag tcaactggct tcagttatgg cgcacgaaat
     1741 ctcccacgtc acccaacgtc acctggcgcg agcgatggaa gatcaacaac gcagcgcgcc
     1801 gctgacctgg gtcggcgcgt taggttctat tttactggcg atggccagtc cgcaggcggg
     1861 gatggcggcg ctgaccggta cactggcggg aacgcgccag gggatgatca gtttcaccca
     1921 gcaaaatgaa caggaagcgg accgcattgg tattcaggtg ctgcaacgct cgggattcga
     1981 tccacaggcg atgccgacct tcctggaaaa attgctcgat caggcgcgtt actcctcgcg
     2041 cccgccggaa attttattga ctcacccgtt gccggaaagt cgtctggcag atgcccgcaa
     2101 ccgtgctaat cagatgcgcc cgatggtggt gcagtcgtcg gaagatttct atctggcgaa
     2161 agcgcgcaca ctggggatgt ataattccgg acgtaaccag ctcaccagtg atttgctgga
     2221 tgaatgggcg aaaggaaacg ttcgtcagca acgagcggcg caatatggtc gtgctttaca
     2281 ggcgatggaa gccaataaat acgacgaggc gcgtaaaacg ctgcaaccgt tactggcggc
     2341 agaacctggc aacgcatggt atctcgatct ggctaccgat atcgatcttg ggcaaaacaa
     2401 agccaatgag gcaatcaatc gcctgaaaaa tgcccgcgat ttacgcacca atccggtgtt
     2461 gcagctcaac ctggcgaacg cttatctgca aggcggtcaa ccacaagaag cggccaatat
     2521 tctgaatcgc tacaccttta ataataaaga tgacagcaac ggctgggatt tgctggcaca
     2581 ggcggaagcc gcgctaaata accgcgatca ggagctggct gcgcgagcag aaggttatgc
     2641 gctcgccgga cgactcgatc aggccatttc gctgttgagt agcgccagtt cgcaggtgaa
     2701 attaggcagc ctgcaacaag cgcgttacga tgcgcgcatc gaccagttgc gccagctgca
     2761 gaaacgcttt aagccttata ccaagatgta attcgcatta aaggaagaac aatgaccaaa
     2821 caggtaaaaa tctaccataa cccacgctgt tcaaagagcc gggaaacgct gaacctgctg
     2881 aaagaaaacg gcgtggagcc agaagtggtg ctctaccttg agacacccgc cgatgcggca
     2941 acgctgcgcg atttgctgaa aatgctgggg atgaatagcg cccgtgaatt gatgcgccag
     3001 aaagaggatc tttataaaga actaaacctg gctgacagtt cgcttagcga agaggcgctg
     3061 attcaggcaa tggtggataa tccgaagctg atggagcgcc cgattatggt cgcgaatggc
     3121 aaagcgcgga ttggtcgtcc accggagcag gtactggaaa tcgtgggtta agatgtgctc
     3181 catcgctagt tgaagcacat tgccggatgc gacgcttgcc acgtcttatc cggcctacga
     3241 atactatgga ttaacttcgc gtaggtcagg cgttcgcgcc gcatccgaca ataaacacct
     3301 tatctacaac ttcagaattt ctttcacaaa cggaatggtc agcttacgtt gcgcggtaat
     3361 cgacgcacga tccaactgat ccaacgtcat aaatagcgtg cgcatttctc tgtcgagccg
     3421 cttcagcaag aaacgcccca catcttccgg cagttcaaaa ccacgcaaac gcgcgcgtaa
     3481 ctgtagcgcc tgcaacttat cttcatcaga aagtggctgc aatttgtaga tctgccccca
     3541 gtcgagacgc gacgcgagat ccggtaatcc cagattcaac tgccgcggtg gacgatcgcc
     3601 ggtgatcaac aaccgtgttt tgcccgattc cagaattcga ttgtagagat cgaaaatcgc
     3661 catctcccac aactcatcgc ctgcaataca ctcaatgttg tcgatacaga ccagcgacaa
     3721 atgctccata ccgtcgagca cttccggaac aaaccaggtg cgtttatcca gcgggacata
     3781 gcccaccgca tcgccacgct gcgacaattc cgcgcaagcc gcgtgcagca gatggctgcg
     3841 ccccgcgcct tcgcgtgccc agagatagat gtaaccgcta tgttcctgac gcagcacgtt
     3901 ttgcagcgcg gccagtaaag aggagttatc ccccggccag aaacttgcaa aggtttcgtc
     3961 gtcaggaaga taaagtggca aagagagctg tgccggtgtg ttcagagata cctcaaccag
     4021 gatttcacaa aatcgcgaga agtttaccac agaatcccat gatgtttgaa ccgggcagca
     4081 acactgcccg gtcggtacat tatttgtctg ttatatccgc gtcttctgcg tccagcacca
     4141 cttcttcagg gcgcagcaca ctgatcagtt tgaaaatcaa acttaagccg ataccgacga
     4201 tggtcgccag cgccatacct tttaactccg ccgcgccgat gtttaccttc gcgccactga
     4261 cgccgatgat caaaatcacg gaagtcagga tcaggttctg tgctttgttg taatccactt
     4321 tcgattcgat caaaacacga ataccggaag caccaatgac accataaagc agcagcgaaa
     4381 cgccgcccat caccggcaat gggatcatct ggatagcggc agccagttta ccgacgcagg
     4441 aaagcaggat agcgaaaatc gccgccccgc cgataaccca ggtactgtaa acacgggtga
     4501 tcgccatcac gccaatgttt tctccgtaag tggtatttgg cgtagagcca aagaagccgg
     4561 agatcacggt cgacaggccg ttagcaaaca tcgaacggtg cagacctgga tcgcgcagca
     4621 gatctttttt gacgatatta gccgttacta ccaggtgccc tacgtgttcg gcaataacca
     4681 ctaacgccgc tggcagaata gtcagaatgg caaaccactc gaagcgcggc gtatagaggg
     4741 ttggcagtgc aaaccagtga gcattaataa tcggcgtggt atcgacaatt cccattgcga
     4801 aagagagcgc gtaccccacc agcacgccaa ttaaaatcgg gataattgcc aggaaaccac
     4861 gaaacagcac ggaacctaaa accgtgaccg ccagggtggt aatagagatg atgatggttt
     4921 tggagtttgg cgtttgccct tcagccggga gtaaacccgc cataccggca gctacgcccg
     4981 ccagctccag accgatgacg gcaacgattg cgcccattgc cgcaggtgga aacagcacgt
     5041 ccaaccagcc ggtccccgct ttcttcacga taaaagaaac caggcagaac agcacgccgc
     5101 acataataaa gccgcccagc gcgacttcat accctaacgg caacagtaac aataccggtg
     5161 aaataaaggc aaagctggaa ccaagataag ccggaatttt ccctttacag atgaagagat
     5221 acagcagcgt tccaataccg ttaaataaca gtacagtcgc cgggttaata tgaaataaga
     5281 cgggcaccag gacggttgca ccaaacatgg cgaacaaatg ttgcaaacta agcgggattg
     5341 tctgtaaaag tggcggtctt tcactcaccc cgatagcacg gcgcgtcata gtattatcct
     5401 ctgtattatg tgttataggc gctttactca aaaaaaagcc gactcttaaa gtcggcttta
     5461 attattttta ttctttattt cgtaccaaag attttgtcac cggcatcgcc gaggcccgga
     5521 ataatgtatc cgtgctcgtt cagtccctgg tcaatcgatg cagtatacag ttcgacgtcc
     5581 gggtgcgctt tttccagcgc agcgatacct tctggcgcag cgaccagcac cagaactttg
     5641 atgctgctgc agcccgcttt tttcagcagg tcgatggtcg cgataacgga accaccggtt
     5701 gccagcattg ggtcaacgat cagcgccata cgctcatcga tgttagaaac cagtttctgg
     5761 aagtacggta ccggctccag cgtttcttca ttacggtaca taccgacaac gctgatgcgc
     5821 gcgctcggaa cgttttccag cacaccgtcc atcataccaa gacccgcacg cagaattggc
     5881 acaacggtaa ttttcttacc tttgatctgg tcgatttcta ccgggccgtt ccagccttcg
     5941 atagttactt tttccgtttc gaggtcggcg gtcgcttcgt aagtcagcag gctacccact
     6001 tcggaagcga gttcgcgaaa gcgcttggtg ctgatatctt gctcacgcat cagtcccagc
     6061 ttgtgtttga cgagtgggtg tttgacttcc acgatcttca tactctttct cctttgaggg
     6121 gcagccacaa aaaaatcgac ggattatacc tcctttcttc agggcggcaa tattattttc
     6181 gttgacttta gtcaaaatga taacagtttg agataaagtt attttatatt caaatggtta
     6241 tgaaagaaga ttattccatc cgaaaactaa cctttgccct ggcacaagtc ttctttcgcc
     6301 gcgcgcccgg ggaaaagacg tgcaaaaagg ttgtgtaaaa cagtctcgca aacgtttgct
     6361 ttccctgtta gaattgcgcc gaattttatt tttctaccgc aagtaacgcg tggggaccca
     6421 agcagtgacc gataaaacct ctcttagcta caaagatgcc ggtgttgata ttgacgcggg
     6481 taatgctctg gttggaagaa tcaaaggcgt agtgaagaaa acgcgtcgtc cggaagtgat
     6541 gggcggtctg ggcggcttcg gtgcgctgtg tgcattgccg caaaaatatc gtgaacccgt
     6601 gctggtttcc ggcactgacg gcgtaggtac caagctgcgt ctggcaatgg acttaaaacg
     6661 tcacgacacc attggtattg atctggtcgc catgtgcgtt aatgacctgg tggtgcaagg
     6721 tgcggaaccg ctgtttttcc tcgactatta cgcaaccgga aaactggatg ttgataccgc
     6781 ttcagcggtg atcagcggca tcgcggaagg ttgtctgcaa tcgggctgtt ctctggtggg
     6841 tggcgaaacg gcagaaatgc cggggatgta tcacggtgaa gattacgatg tcgcgggttt
     6901 ctgcgtgggc gtggtagaaa aatcagaaat catcgacggc tctaaagtca gcgacggcga
     6961 tgtgctgatt gcactcggtt ccagcggtcc gcactcgaac ggctattcgc tggtgcgcaa
     7021 aattcttgaa gtcagcggtt gtgatccgca aaccaccgaa cttgatggta agccattagc
     7081 cgatcatctg ctggcaccga cccgcattta cgtgaagtca gtgctggagt tgattgaaaa
     7141 ggtcgatgtg catgccattg cgcacctgac cggcggcggc ttctgggaaa acattccgcg
     7201 cgtattgcca gataataccc aggcagtgat tgatgaatct tcctggcagt ggccggaagt
     7261 gttcaactgg ctgcaaacgg caggtaacgt tgagcgccat gaaatgtatc gcaccttcaa
     7321 ctgcggcgtc gggatgatta tcgccctgcc tgctccggaa gtggacaaag ccctcgccct
     7381 gctaaatgcc aacggtgaaa acgcgtggaa aatcggtatc atcaaagcct ctgattccga
     7441 acaacgcgtg gttatcgaat aatgaatatt gtggtgctta tttccggcaa cggaagtaat
     7501 ttacaggcaa ttattgacgc ctgtaaaacc aacaaaatta aaggcaccgt acgggcagtt
     7561 ttcagcaata aggccgacgc gttcggcctt gaacgcgccc gccaggcggg tattgcaacg
     7621 catacgctca tcgccagcgc gtttgacagt cgtgaagcct atgaccggga gttgattcat
     7681 gaaatcgaca tgtacgcacc cgatgtggtc gtgctggctg gttttatgcg cattctcagc
     7741 ccggcgtttg tctcccacta tgccgggcgt ttgctgaaca ttcacccttc tctgctgccg
     7801 aaatatcccg gattacacac ccatcgtcag gcgctggaaa atggcgatga agagcacggt
     7861 acatcggtgc atttcgtcac cgatgaactg gacggtggcc cggttatttt acaggcgaaa
     7921 gtcccggtat ttgctggtga tacggaagat gacatcaccg cccgcgtgca aacccaggaa
     7981 cacgctattt atccactggt gattagctgg tttgccgatg gtcgtctgaa aatgcacgaa
     8041 aacgccgcgt ggctggatgg tcaacgtctg ccgccgcagg gctacgctgc cgacgagtaa
     8101 tgcccccgta gttaaagcgc cagctctgcc gctggcgttt ttcaattcac ctgtaaatcg
     8161 caagctccag cagttttttt cccccttttc tggcatagtt ggacatctgc caatattgct
     8221 cgccataata tccaggcagt gtcccgtgaa taaaacggag taaaagtggt aatgggtcag
     8281 gaaaagctat acatcgaaaa agagctcagt tggttatcgt tcaatgaacg cgtgcttcag
     8341 gaagcggcgg acaaatctaa cccgctgatt gaaaggatgc gtttcctggg gatctattcc
     8401 aataaccttg atgagttcta taaagtccgc ttcgctgaac tgaagcgacg catcattatt
     8461 agcgaagaac aaggctccaa cgctcattcc cgccatttac tgggcaaaat tcagtcccgg
     8521 gtgctgaaag ccgatcagga attcgacggc ctctacaacg agctattgct ggagatggcg
     8581 cgcaaccaga tcttcctgat taatgaacgc cagctctccg tcaatcaaca aaactggctg
     8641 cgtcattatt ttaagcagta tctgcgtcag cacattacgc cgattttaat caatcctgac
     8701 actgacttag tgcagttcct gaaagatgat tacacctatc tggcggtgga aattatccgt
     8761 agcgatagca tccgttacgc gctgctggag atcccatcag ataaagtgcc gcgctttgtg
     8821 aatttaccgc cagaagcgcc gcgtcgacgc aagccgatga ttcttctgga taacattctg
     8881 cgttactgcc ttgatgatat tttcaaaggc ttctttgatt atgacgcgct gaatgcctat
     8941 tcaatgaaga tgacccgcga tgccgaatac gatttagtgc atgagatgga agccagcctg
     9001 atggagttga tgtcttccag tctcaagcag cgtttaactg ctgagccggt gcgttttgtt
     9061 tatcagcgcg atatgcccaa tgcgctggtt gaagtgttac gcgaaaaact gactatttcc
     9121 cgctacgact ccatcgtccc cggcggtcgt tatcataatt ttaaagactt tattaatttc
     9181 cccaatgtcg gcaaagccaa tctggtgaac aaaccactgc cgcgtttacg ccatatttgg
     9241 tttgataaag cccagttccg caatggtttt gatgccattc gcgaacgcga tgtgttgctc
     9301 tattatcctt atcacacctt tgagcatgtg ctggaactgc tgcgtcaggc ttcgttcgac
     9361 ccaagcgtac tggcgattaa aattaacatt taccgcgtgg cgaaagattc acgcatcatc
     9421 gactcgatga tccacgccgc acataacggt aagaaagtca ctgtggtggt tgagttacag
     9481 gcgcgtttcg acgaagaagc caacattcac tgggcgaagc gcctgaccga agcaggcgtg
     9541 cacgttatct tctctgcgcc ggggctgaaa attcacgcca aactgttcct gatttcacgt
     9601 aaagaaaacg gtgaagtggt ccgttacgca cacatcggga ccgggaactt taacgaaaaa
     9661 accgcgcgtc tttatactga ctattcgttg ctgaccgcag atgcgcgtat caccaacgaa
     9721 gtacggcggg tatttaactt tattgaaaac ccataccgcc cggtgacatt tgattattta
     9781 atggtgtcac cgcaaaactc tcgccgtctg ttatatgaaa tggtagaccg cgaaatcgcc
     9841 aacgcgcagc aagggctgcc cagtggtatc accctgaagc taaataacct tgtcgataaa
     9901 ggcctggttg atcgtctgta tgcggcctcc agctccggcg taccggttaa tctgctggtt
     9961 cgcggaatgt gttcgctgat ccccaatctg gaaggcatta gcgacaacat tcgtgccatc
    10021 agtattgttg accgttacct tgaacatgac cgggtttata tttttgaaaa tggcggcgat
    10081 aaaaaggtct acctttcttc cgccgactgg atgacgcgca atattgatta tcgtattgaa
    10141 gtggcgacac cgctgctcga tccgcgcctg aagcagcggg tgctggacat catcgacata
    10201 ttgttcagcg atacggtcaa agcacgttat atcgataaag aactcagtaa tcgctacgtt
    10261 ccccgcggca atcgccgcaa agtacgggcg cagttggcga tttacgacta catcaaatca
    10321 ctcgaacaac ctgaataacc ctatgccaat acacgataaa tcccctcgtc cgcaggagtt
    10381 tgctgcggtc gatcttggtt caaacagttt tcacatggtc atagcccgtg tggtagatgg
    10441 tgccatgcag attattggcc gcctgaaaca gcgggtgcat ctggcggacg gcctggggcc
    10501 agataatatg ttgagtgaag aggcaatgac gcgcggtttg aactgtctgt cgctgtttgc
    10561 cgaacggcta caagggtttt ctcctgccag cgtctgtata gtcggcaccc atacgctgcg
    10621 tcaggcgctg aacgccactg actttctgaa acgcgcggaa aaggtcattc cctacccgat
    10681 tgaaattatt tccggtaatg aagaagcccg tctgattttt atgggcgtgg aacataccca
    10741 accggaaaaa ggtcgcaaac tggttattga tattggcggc ggatctacgg aactggtgat
    10801 tggtgaaaat ttcgaaccta ttctcgttga aagccgccgg atgggttgtg tcagctttgc
    10861 ccagctttat tttcctggcg gggtcatcaa taaagagaat tttcagcgcg ctcgcatggc
    10921 ggcagcacaa aaactggaaa ctttaacctg gcaattccgt attcagggct ggaacgttgc
    10981 aatgggcgct tccggtacca taaaagccgc ccatgaagtg ttaatggaaa tgggcgagaa
    11041 agacgggata attaccccgg aacgtctgga aaaactggta aaagaaattt tacgtcaccg
    11101 taatttcgca tcgctgagtt taccgggtct ttccgaagag cggaaaacag tcttcgttcc
    11161 tggactggcg attttatgtg gtgtgtttga tgctttagcc atccgtgaac tgcgcctttc
    11221 tgacggggcg ttacgcgaag gcgtactgta tgaaatggaa ggacgtttcc gtcatcagga
    11281 tgtgcgtagt cgcaccgcca gcagcctcgc caaccagtat cacatagaca gcgaacaggc
    11341 ccgacgagtg ctggatacca ctatgcaaat gtacgaacag tggcgggaac agcaaccgaa
    11401 gctggcgcat ccgcaactgg aggcgctact gcgatgggcc gccatgctgc atgaggtcgg
    11461 gttgaatatc aaccacagcg gtttgcatcg ccactccgct tatattctgc aaaacagtga
    11521 cttgccgggt tttaatcagg aacagcagct gatgatggcg acactggtgc gctatcaccg
    11581 taaagcgatt aagctcgacg atctgccgcg ctttaccttg tttaagaaga aacagttcct
    11641 gccactgata cagctattgc gccttggcgt attactaaac aatcaacgtc aggcaaccac
    11701 cacaccgcca acattgacac tgattactga tgacagtcac tggacactgc gtttcccgca
    11761 tgactggttt agtcagaatg cgctggtact gcttgatctg gaaaaggagc aagaatactg
    11821 ggaaggcgtg gctggctggc ggttgaaaat tgaagaagaa agtaccccag aaatcgccgc
    11881 ttaatgcttt gcgggccgac gagaaatgtc ggcccgcatt attcaggcac tttcgcgaat
    11941 gggttcgatt tcattcagcg tatcaattaa cggttgcggc ttaccaataa gataaccctg
    12001 catataatcg atccccaaag agagcaccgc ctcgcggatc tcttcgtttt caacgtactc
    12061 tgccactacc cgcattttct tcattcgcgc cagatggcaa atcgacgcca caatctgata
    12121 atccagacta tttgacacaa tattgcggat aaagctgccg tcaattttaa gcagatcggc
    12181 attcacattt ttaagccgtg catagctggc gtagccggtg ccgaaatcat caatcgcaat
    12241 ctggcagcct aattcctgaa gatgctgcaa ggtgatttgc gcctgcttaa cattggtcag
    12301 agcattactt tcggtgactt caaaaataag ttgccacgct tcaatctgat atttagccag
    12361 caactgactg acttcaacag gaaaacgagc ctgacatacc gaggttggag acagattaat
    12421 agcaaaacgg tgagcgggca tcttcgctct gttttcagcc ataaattgca gcgtatgctc
    12481 aatgacccac atgtcgatac tcgacgataa accaaattcg tgcgcgaccg gcaagaagct
    12541 accggggccg atcagttcat cattctcacc tttcatgcgc agaagaattt catggtaaac
    12601 atcaccacgc ataccggtaa tcggctgggc catcaggaaa aaatggttgt gttccagcgc
    12661 ctgctgtagt cgattcatca tcgcgacttt atctttcaat tcgcgttgca aatacattgc
    12721 cccgcaacgc tgcatatttt ccggggcgtt ggtcacgatg gaaagttcgg cgaccgtatt
    12781 tagctctccc agcagcaggt agatatgatt cactggcgag cgcacatagc agtaactgac
    12841 gccaatctgc ggttgcatcg gcatcccatc ccaaaagaaa cgaaattgct tgagatgact
    12901 atccagtgcg gtaatgcgct cctggtgcga ttctgtattc agtcgcagcg cgagatcgtt
    12961 acccgaaagc tgataaacat cttcacccgg ttccagcaag ggtgacagcc agtgagaaag
    13021 tttttgctt
//
LOCUS       DAZSNM010000113        13004 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZSNM010000113 DAZSNM010000000
VERSION     DAZSNM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13004
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.113"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..309)
                     /locus_tag="ACHW42_002616"
     CDS             complement(<1..309)
                     /locus_tag="ACHW42_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFR8944385.1"
                     /translation="MAISDTKLRTIYGKPYSGPQELADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     Y"
     gene            complement(515..1234)
                     /locus_tag="ACHW42_002617"
                     /pseudo
     CDS             complement(515..1234)
                     /locus_tag="ACHW42_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1250..1582)
                     /gene="cusF"
                     /locus_tag="ACHW42_002618"
     CDS             complement(1250..1582)
                     /gene="cusF"
                     /locus_tag="ACHW42_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR8944386.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1606..2988)
                     /gene="cusC"
                     /locus_tag="ACHW42_002619"
     CDS             complement(1606..2988)
                     /gene="cusC"
                     /locus_tag="ACHW42_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR8944387.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3145..3828
                     /gene="cusR"
                     /locus_tag="ACHW42_002620"
     CDS             3145..3828
                     /gene="cusR"
                     /locus_tag="ACHW42_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR8944388.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3818..5260
                     /gene="cusS"
                     /locus_tag="ACHW42_002621"
     CDS             3818..5260
                     /gene="cusS"
                     /locus_tag="ACHW42_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR8944389.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5410..7647
                     /gene="nrfB"
                     /locus_tag="ACHW42_002622"
     CDS             5410..7647
                     /gene="nrfB"
                     /locus_tag="ACHW42_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFR8944390.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7634..10602
                     /gene="nfrA"
                     /locus_tag="ACHW42_002623"
                     /pseudo
     CDS             7634..10602
                     /gene="nfrA"
                     /locus_tag="ACHW42_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10603..11493
                     /locus_tag="ACHW42_002624"
     CDS             10603..11493
                     /locus_tag="ACHW42_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR8944391.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11676..12437
                     /gene="envY"
                     /locus_tag="ACHW42_002625"
     CDS             11676..12437
                     /gene="envY"
                     /locus_tag="ACHW42_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR8944392.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12483..12559)
                     /locus_tag="ACHW42_002626"
     tRNA            complement(12483..12559)
                     /locus_tag="ACHW42_002626"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12523..12525),aa:Arg,seq:tct)
     gene            12802..>13004
                     /locus_tag="ACHW42_002627"
     CDS             12802..>13004
                     /locus_tag="ACHW42_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFR8944393.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3183 a         3373 c         3301 g         3147 t
ORIGIN      
        1 gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa aataagtgcg
       61 tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc tggcatcctt
      121 caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat gccagcgata
      181 gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc catcagcatc
      241 agccaattct tgtgggccag aatatggttt accataaata gtacgcagtt ttgtgtcgct
      301 tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac atctggtaca
      361 caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg tagacatact
      421 caggtgaaaa aaaggttgtt tttcgagtta taacagacag ttaattaact acagtggaca
      481 atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg accacgttat
      541 ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca tcaatgggcg
      601 ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga atatccgcct
      661 ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc gccgtaccgc
      721 cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga atggtcaggt
      781 cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac accttgtcga
      841 taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa ctgacattcg
      901 tcgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt ttcaccccca
      961 gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct tcatcggcat
     1021 atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg aaccgcgtat
     1081 tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc attttttctg
     1141 cgtgcggttc cgcctagtca aaccaggtaa aacctgccgc tgaaataata ccgcccgcga
     1201 tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt tactggctga
     1261 ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac gccactttgt
     1321 cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc atggtcatct
     1381 ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg ctttccagat
     1441 caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg ctcatggttt
     1501 catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga ctgaacattg
     1561 cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt gctgccaacc
     1621 gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat tcaggtcaag
     1681 taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca gataacttac
     1741 cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg acgccagata
     1801 acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg catctgccac
     1861 ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct gctggcgaat
     1921 ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga tctcaatttt
     1981 aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat cgctgctggc
     2041 ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg cagcaccaat
     2101 attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc gctgcaataa
     2161 gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc tgtttactgt
     2221 ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat tattcgcctg
     2281 cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca ccccgcgagc
     2341 ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga caaacgcata
     2401 tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat acgccagctg
     2461 ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg cgcgctgggc
     2521 ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta agcgaccgaa
     2581 aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg tggctgaatc
     2641 gcctttaaga ttgccgctcc agctgccgct gccctcgcca ttgagctgtg ggtagcggtc
     2701 ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg ccatgcgcaa
     2761 atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat caacaaaaaa
     2821 ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt tctggctgag
     2881 tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca gtgaacaacc
     2941 agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag gctcataatt
     3001 tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg tgacaggaaa
     3061 atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta ctctagaatt
     3121 gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga aaagaaaacc
     3181 ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt ggccgacaac
     3241 gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat cctcgatatt
     3301 atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc caataaaggg
     3361 atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa ggggctggag
     3421 ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct ggcgcgggtg
     3481 cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca ggttgccgat
     3541 ctgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat cactctgacc
     3601 agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt gctgccccgc
     3661 tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa cgccattgat
     3721 gtggcggtga agctgctacg cggcaaaatc gacaacgact ttgagccgaa gctgattcaa
     3781 accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc catttcagcg
     3841 cccgttttcg ctggcaaccc gcctgacctt ttttatcagc caggccacca tcgcggcgtt
     3901 tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg agcaggacat
     3961 taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc ctgacgaaac
     4021 gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca acgtgttgat
     4081 ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc ctgatatccg
     4141 cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg tgtatctcct
     4201 ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac acagcaactg
     4261 gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt atacgctcta
     4321 catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga ataaacttat
     4381 tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg cggtacataa
     4441 aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct cgaaagatct
     4501 cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg tactgtcgtt
     4561 caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt tctcagcgga
     4621 tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg aaatcgccct
     4681 cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc tcgaagagct
     4741 gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg ataacaacca
     4801 gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag tgttcgattt
     4861 tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg acaaacgtca
     4921 ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt ctaatgccct
     4981 tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg atcaccaggt
     5041 gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac cgcgattgtt
     5101 tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta gcggtattgg
     5161 gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg taacgtcaga
     5221 tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca aacggcaact
     5281 tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg gtaaaaagct
     5341 gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc gttaatgtaa
     5401 ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg cttaaaagta
     5461 atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt ttttattgat
     5521 gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg ctacccgcga
     5581 atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat ggttccggcg
     5641 tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac gctcgactac
     5701 gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca acgtgatgtt
     5761 gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg tcctggtccc
     5821 accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt tgaacgtagc
     5881 gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat ttcaccgatg
     5941 gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat cccagtgtat
     6001 ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga gttttcagaa
     6061 ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc cagcgcaggc
     6121 gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg taacggtatt
     6181 gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct gaaagaaaaa
     6241 ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga acgcgagcag
     6301 cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga atatttcccc
     6361 gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat tgttttccag
     6421 ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct ctggcgcgac
     6481 cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat gatccagctt
     6541 ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct ggcatttcct ttctattttt
     6601 agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat ggttaaccgc
     6661 atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg cctgctttcc
     6721 gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg cgtgctgaaa
     6781 caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac gcatgacttc
     6841 cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct gctggaaaat
     6901 caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga aggtctacgc
     6961 ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc acaggcgctg
     7021 gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc gtcctcgctg
     7081 attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct gcgtctggaa
     7141 aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct ggcggccctg
     7201 acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca aattgtcaca
     7261 gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat gttgtacaat
     7321 gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg gcaatatgtg
     7381 ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa tcgttcagca
     7441 attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa gtttttggtc
     7501 accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca acgcgaatta
     7561 caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca ggttgcgcaa
     7621 ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt ctggtttact
     7681 gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca gcgcagaaga
     7741 gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata aggcgctgaa
     7801 ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata tacaccaaca
     7861 ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc attttggtca
     7921 tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag gagatgcccg
     7981 acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga ctgttgaaga
     8041 actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc gcagtgaagt
     8101 cgggcagaat gccctgcggc tggcacaatt acctgtcgcc agagcgcaac tgaacgatgc
     8161 gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc aacgggcaat
     8221 ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc agcagaacac
     8281 attaagcgcg gcagaacgcc gtcagtggtt tgacgtgctt cttgccgggc agctggacga
     8341 tcggatcctg gcactgcaat cacaggggat cttcaccgat cctcagtcat atattactta
     8401 cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc tcattgaaaa
     8461 taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt tatctaaata
     8521 cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg ataaccgcca
     8581 gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg acgcagcgca
     8641 aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg ctgtcagcgt
     8701 ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc agcaagaacc
     8761 gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg agcagtcacg
     8821 cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc gtgtcagcca
     8881 gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag caacgccggc
     8941 gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt ggcaaagtca
     9001 gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg atatgtcgcc
     9061 ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca cgctacccgg
     9121 tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct ggcaacatcg
     9181 tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg cggcctggca
     9241 gaaaatcagt cttcacgaca tgagcaatga tgatctgctc gccgccgcca acaccgctca
     9301 ggcagcggga aatggcgcag ctcgcgatcg ctggcttcaa caggcagaac aacgtgagct
     9361 gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag ctcagccaga
     9421 actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca actcttacgt
     9481 tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga gtgatttgcg
     9541 cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg gttacgcctt
     9601 gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc ataaagggct
     9661 tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc tggatgacat
     9721 gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc aggcataacc
     9781 ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca tgaggaggtc
     9841 ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg cgcaatgagt
     9901 accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag ctacggacaa
     9961 ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct gctctcagtt
    10021 tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt gaaaaatccg
    10081 atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt cctcgccgtc
    10141 gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct gcgcgccagc
    10201 gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg ttcaggctgg
    10261 tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat tcaggcgtgg
    10321 acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac tattgagcct
    10381 tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc gcagcttggc
    10441 ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc ctggccgcac
    10501 aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca acgtaacgga
    10561 gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa gttcattttc
    10621 gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg tattatctgg
    10681 caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct gatgagtcag
    10741 ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg cgatgcattt
    10801 acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca acagactggc
    10861 ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca gaaacagtcg
    10921 tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca gcaagccaga
    10981 ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc ggaaattgac
    11041 gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt aaacaacgcg
    11101 cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt tttcgccgga
    11161 aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac cggagttaat
    11221 gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg tgaacgttat
    11281 ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt ttatgaactg
    11341 tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc agaaattgcc
    11401 tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc tctgcgctat
    11461 ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt cgggtacgac
    11521 ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta aaaagcatgc
    11581 taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa gcatagatcc
    11641 tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc agtgaacctt
    11701 gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat gctacgttta
    11761 cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt gaactctcaa
    11821 cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat tatctgttgt
    11881 ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctagcaacc ccggttatcg
    11941 cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc aagcagcaat
    12001 ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta ttgtctaact
    12061 ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc agcgtccgcg
    12121 acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg cgaattgtcg
    12181 ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac gaaaatacca
    12241 gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg ttattgatgg
    12301 ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg tcgtacttta
    12361 tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc aaacagcgac
    12421 aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa atgaagaaga
    12481 aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc gttgctctat
    12541 ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga attatacggt
    12601 caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac gaccgcgtct
    12661 gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg cgtctgaatc
    12721 aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg aaaattgtcc
    12781 gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga tactcatcct
    12841 atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca gattgtcctg
    12901 aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt tgatttaatc
    12961 attatggata tagacttgcc cggaacagac ggttttacct tcct
//
LOCUS       DAZSNM010000114        12940 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZSNM010000114 DAZSNM010000000
VERSION     DAZSNM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12940
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.114"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(165..1616)
                     /gene="uxaB"
                     /locus_tag="ACHW42_002628"
     CDS             complement(165..1616)
                     /gene="uxaB"
                     /locus_tag="ACHW42_002628"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR8944394.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1843..3261)
                     /locus_tag="ACHW42_002629"
     CDS             complement(1843..3261)
                     /locus_tag="ACHW42_002629"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR8944395.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3415..3759)
                     /locus_tag="ACHW42_002630"
     CDS             complement(3415..3759)
                     /locus_tag="ACHW42_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR8944396.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3759..4685)
                     /gene="glsB"
                     /locus_tag="ACHW42_002631"
     CDS             complement(3759..4685)
                     /gene="glsB"
                     /locus_tag="ACHW42_002631"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR8944397.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4749..5690)
                     /locus_tag="ACHW42_002632"
                     /pseudo
     CDS             complement(4749..5690)
                     /locus_tag="ACHW42_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            5764..6879
                     /gene="yneK"
                     /locus_tag="ACHW42_002633"
     CDS             5764..6879
                     /gene="yneK"
                     /locus_tag="ACHW42_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR8944398.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            7029..8219
                     /gene="ydeA"
                     /locus_tag="ACHW42_002634"
     CDS             7029..8219
                     /gene="ydeA"
                     /locus_tag="ACHW42_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR8944399.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8244..8909)
                     /gene="marC"
                     /locus_tag="ACHW42_002635"
     CDS             complement(8244..8909)
                     /gene="marC"
                     /locus_tag="ACHW42_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR8944400.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9117..9551
                     /gene="marR"
                     /locus_tag="ACHW42_002636"
     CDS             9117..9551
                     /gene="marR"
                     /locus_tag="ACHW42_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR8944401.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9571..9954
                     /gene="marA"
                     /locus_tag="ACHW42_002637"
     CDS             9571..9954
                     /gene="marA"
                     /locus_tag="ACHW42_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR8944402.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9986..10204
                     /gene="marB"
                     /locus_tag="ACHW42_002638"
     CDS             9986..10204
                     /gene="marB"
                     /locus_tag="ACHW42_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR8944403.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10235..11134)
                     /gene="eamA"
                     /locus_tag="ACHW42_002639"
     CDS             complement(10235..11134)
                     /gene="eamA"
                     /locus_tag="ACHW42_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFR8944404.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11401..12516
                     /gene="ydeE"
                     /locus_tag="ACHW42_002640"
                     /pseudo
     CDS             11401..12516
                     /gene="ydeE"
                     /locus_tag="ACHW42_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            12643..12738
                     /gene="mgtS"
                     /locus_tag="ACHW42_002641"
     CDS             12643..12738
                     /gene="mgtS"
                     /locus_tag="ACHW42_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFR8944405.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            12738..12841
                     /gene="mgtT"
                     /locus_tag="ACHW42_002642"
                     /pseudo
     CDS             12738..12841
                     /gene="mgtT"
                     /locus_tag="ACHW42_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
BASE COUNT         3407 a         3305 c         2958 g         3270 t
ORIGIN      
        1 ggacaggggt atcgcttagt ttttcaattt cctatatgtc aagcttacaa cattaacagg
       61 cgctgcatgt gacacctgtt aatgataaag gaatatagga tgttgtaact aacatagtga
      121 aataagaaac ccggtcgaaa ccgggttcag aagtaacggt gttattagca caacggacgt
      181 acagcttcgc gcatcccttt ttcaagaatc gcatccaggt cattagcaac ctgctcgacc
      241 agaccaggca cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa cacaatcgct
      301 accagttcct gggtaccgat aacacggtca cgatgctggc tccagagttg ctgataacgt
      361 tccagccagt gtgcatcgtc ctgtaccgga tatgtttctc cgttacgctc accacgatag
      421 aacgcaatca atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc atttgccttc
      481 tgccctgcca gcagctgcgg caggatgcgg gtgcggaact tggtcatacc gttgagcgcg
      541 atagacagca gctgatgctt aatgtacggg ttacggaaac gcccggtgac tgcactggcg
      601 aaagattcca gttcatcacg aggcaaatcc agtaccggaa taatttcttc gtagatggct
      661 ttttcaacga atgcgcaaat ttcagcatcg ttcatcgctt cacctacggt atccagccct
      721 gcctggaacg ccaccggcac cagcgcggtg tgcgcaccgt tgaggattgc cactttgcgt
      781 tctttatacg gtttaatatc gtcaacaatc agcacgttga gcggatattt gtccaggcgc
      841 agctcagtcg ctaaggattt cggcccctga atcacaaaca ggtaaaagtg ttcagcggtg
      901 tcgaggaaac catcgtgata acccagttct tcttccagtt tagccacttc atcgcgcgga
      961 taaccggtaa cgatacggtc caccagcgta gaacagaagc tgttagcctg atccagccat
     1021 tgaataaatg cttctggcag tgcccactct tgcgcatagc gcagcaccag ttcacgcaac
     1081 gcgtcgccat tgtagtcaat caactcacac ggaatgatga tccaaccttt atccagcgca
     1141 ccgttgaaat ggctaaagcg ttcgaacagc agacgggtca gttttgccgg atagcttacc
     1201 gctggcgcgt catcgaattt atcgcccgca tggtagctga tacccgcttc ggtggtgtta
     1261 gagaaaacaa agcgcatttc cgggttgtgc gccagtttca ggaattcatc gtattcactg
     1321 tagacgctga tttcacgatt aaccgagcga atcagacgcg cgtcgctgac cgcttccccc
     1381 ttctcattca ggccacggat aatggtggtg tacagaccat cctgcgtgct cagtgacggc
     1441 gggaatgaag tttcaatcgg acgaacaacg accacgccag aattcagatc ggtgtgctca
     1501 ttcaggagat cgatttgcca gtcaacaaag gcgcgcagga agttaccttc accaaactga
     1561 atgatacgtt ctggatactg tgcaccggga aaatcgcgac gatttagtgt tttcacaatg
     1621 ggttcccttc tgattagtca cacaacccgt ttgaattggt gcgacaggtt agcaaacttt
     1681 aatacgccga acccctgttt tgatcaactc ctgatgatta atgagcagtt ttatgagaaa
     1741 agtgtggcgc ggatcatggt ttaatcgagg aaatacgcct tttcctggat cataaagtgg
     1801 tagaacacat tgcattcaaa tcgcgcggaa tgaataaaga tgtcagacaa cttcctcacc
     1861 gtaacgcata gtgctggtac ggttgcgccc atctttcttc gagcgataca gacacgtatc
     1921 ggcttcgacc atcaatttat taaaatcatc ggtcagcgtt cggtacgatg cgcgaccact
     1981 accgacgcca atacttaccg tgagataaag cgttttttgt tgccaggtga atggttgcag
     2041 ttcaacgcct ttacggattt tttccgccat tagcagacca tcgacaggat tcaccgacgg
     2101 cactgcaaca gcaaattctt cgccgcccat gcgcgccacc agccccttat caccgacaat
     2161 cttctgaata tgccgggcaa acacgcttaa cactttatcg ccacattcat gtccatagtt
     2221 atcgttaatg cttttgaaat agtcgatatc aagcagcatg accgtcagat gttgtgtctg
     2281 tttcagcgat ggacttttca gcgcctcata aagaccggac cgtgagtaaa cctgagtcag
     2341 aaaatcaaag tcggctcgca gcgcaacttg cttcattagc aaattgatcg ctgccacgct
     2401 aaaagaaacc ataattgggc atatcgccat cgtggcaata ccgagacgtg cggagaacat
     2461 ttccggaatg gagaacggcg aaccgaccga gatatcaatc accgaatttg ccaccagtac
     2521 gatttccacc gcaccggtga caaaggtcaa cagacatgtt acctgcggcg tatagcgcac
     2581 tgcacaccag attaatgcag gcagcgggaa cgccagactc cccgctccgc caatgactac
     2641 cgaggcaata accgacacaa taaacgcgat ggcgggcatc atctgttctg ctttaaagcg
     2701 cggcagcact ccaggaatag ccaacgtcag catacaaggc acgatcaaca cgcccgttga
     2761 gaattgctca ctgaaccagt ccgcaagcaa aggccagaaa tccagactat caatactgac
     2821 cgaaccaatc gccccgacaa tagcgcataa taatgcggca atcagacagt aattaaatag
     2881 ccgtaaagca cttactggct catacttatt tttccccaga cgcttatccc gcacgaccag
     2941 taaggcgacg gtaacaataa acatcatatt ggagaaattg ataaccagtg aaaccagtcc
     3001 ccattcggtg gttatggcat cataaaccag catcgccaca taactgatcg catagtaatg
     3061 cagtcgatta agccagacat agcgggcaaa cacccccgcc attacgccat tcagcggcca
     3121 gaaaagagaa agttcgtgaa ccagccgtaa ctctgcacca ataaaataga acagcgtggt
     3181 tagcgcaaaa atagcgatgc tattacgtag cagatgacct tcctggaaca aacggaaagt
     3241 tgaaatgggg tgtacatgca ttaattatct gtccatacaa ttgcgggtgg caattatctc
     3301 aacgtgataa gtaaattctc ataaaggata gcatgttcgc taacagcggc ggcaaataaa
     3361 ggtaccctgg gaatgctatt ttatcgtttt ccgcaattct catggctgat tcatctatac
     3421 aaccagccag tgatagatta ccgccacgat ataacgttgt tgttcttcgc ttaacgaaac
     3481 accctgcgga atgttatgcc acttagccag acaaccgcga cagcaggtcg cggtggcgtg
     3541 ctgagcgata aacaccggat gcccgcgcat gggggtttgc ttaccgtcat tagccggtaa
     3601 tgccggagca agacgtttag cgacaaaatc cgccgcatgt tgttcgatga ctggcgcgcc
     3661 tttctccagg caatactgac gctctttcat gcccagacga aagcgagagc gaaattttga
     3721 acgggataaa cgcgcgaaga gtggatcgag agactgcatt aataaaccga acgccctaac
     3781 tgtttcgtca attgttcaag gacggcgata cccgcaagcg agttacctgc atcatccagt
     3841 tccggactcc agactgcgat ggccatttca tgcggaacaa tcgccacaat accgccacca
     3901 acgccagatt tcgccggtag tcccacccgc caggcaaact cccccgcgtt ctggtacata
     3961 ccactggtcg ccatcagcgc gttaatttgc cgcgcctgca ttggcgtcac cacaggttca
     4021 tcaatatgaa tagctttccc ctgattagcc agaaagacaa acgtccgggc cagctctaca
     4081 cagctcattt tcagagcgca gtaatgaaag tagttttgca gaacggttgt cacgtcatga
     4141 tggaaattgc caaacgactt catcagccag gcgatagccg catttcgcgc ggaatgttca
     4201 aattcggaac gcgctaccac cgtatcgtat gaaatatcag acacaccgct taagccgcgc
     4261 acgacttcca gcatacgttg ccgtggtgcg cttagtcgcc cttgcaacat atcgcagacc
     4321 accagcgcac cggtattaat gaacggatta cgcggaatac cctgttccat ttccagttgc
     4381 actaaagaat tgaacggtga tccagacgga tctttgccga cgcgttgcca gatttcctct
     4441 tcggagtaat gacgcatggc gacaacgaga ctcagcactt tggaaataga ctgaatggaa
     4501 aaacgttctt gcgcgtctcc ggcctgaaaa agctgtccgt caacggtaca gatagcaatc
     4561 cccaatcggg aaccgtctac tgtagccagc gccggaatat aatccgcgac tttaccctga
     4621 ccaatgagcg gccgcacttg ccgcaagatg ttttctaaaa ttgcattatc catggcgact
     4681 gccactttct actcctggac cgcaggtctg aaaagacctg cgagtatatc agagctgaat
     4741 atgtcgcgtc agatccggtc tttccacact gtctggatat tacagaattc gtgtaagccg
     4801 aaatgggaaa gctcacgacc aaagccactc tttttcacgc caccaaaggc cactcgcgcg
     4861 tcgctggcac aataaccatt gataaacacc ccaccgcatt ccagacgtgc cgccatctgt
     4921 ctggcctgtg tttcgtcggt ggtaaaaatg gtcgctgaaa ggccgaactc actatcatta
     4981 gccagttcca gcgcatgttc tgcatctttc gcaatggtga ttgcagcaac ggggccaaac
     5041 atttcttccc gaaacgcggt catttctggg gtaacattcg ccagaaccgt tggcggatag
     5101 tagttacctg ctccagccat cttttccccg cccagtaaca aacgcgcacc ctgcgccagg
     5161 gttttctcca cctgatgatg cagctcatca cgtaaatcaa aacgagccat tggtccgaga
     5221 gcgttctctt catcacgggg atcgcccatt ttcaaggctg ccgcagctga cacaaaacgt
     5281 tcggtaaatg ccgaagcaat tccctcttcg ataataaagc gttttgccgc tgcacaaacc
     5341 tgtccggtat tctgataacg tccggctacc gccgctttaa ccgccagttc cagatcggca
     5401 tcgttaagca caataaatgg atccgaaccg cccagttcca gtacgcattt tttcagtgcc
     5461 gctccagcac gaacacttcc ggtcaccgtg acagcagcaa tgcgcgagtc tttaatcatc
     5521 tgactgacac cgtcgttgtc ggcattcagc cagccatata cgccttgcgg gattcccgca
     5581 tctttaaaca cttgggcaat gagctgagca cagcccatca cattcggcgc atgtttaagt
     5641 aagtagccgt tacctgcaag aatgatggga acagcgccac gcatcacctg cctttgatag
     5701 ttcttgcagc tcaaatgccc tatatagtga tgttccgatg acttatgact atatggggca
     5761 aatatggtta cgccagtaag catctgcaat tacatatctc ttcctgatga ttttcccgct
     5821 cgtaatattg cacctcaagt aaaagaagtt ttaaaggatt ttattgatgc actcagtacc
     5881 ataatatgtg atgaagagtg gcgcacgtct ttaaacacca actcagccac aaaaaagata
     5941 tttaacaacc ttgataactt atcatatatt cagaggactt cttttcgggg taacgacaca
     6001 ctatacaatg aaaaggttca gtttaaactc acttatcctg tcaagaatgg aagacacaaa
     6061 gaaaatattg aatttcaggt agtaataaat ttaagcccta tttatttaga taattttcgc
     6121 catgatggag aaattaatat tttttgcgct cccaacccaa agcctgtcac tatggggcgc
     6181 gtatttcaga ccggcgtcga gcgtgtgctc tttctgttta tgaatgattt tattgaacaa
     6241 tttccaatga tcaaccttgg cgcccccatc aaaagagcgc atacaccaca tattgaaccc
     6301 ctgcctccgg atcaccatac cgctgcagat tacttacgcc agtttgattt gcttgtcctg
     6361 aattttatct ctcgcggtaa ttttgtcata ctcccccgat tatggaataa ctctgaggtt
     6421 cacagatggt tcgtcaataa agatcctaac ttgatcaccg ccattctcga cataacggac
     6481 agcgaattaa aagaggattt attgcaaagc ctgatggatt cattaggttc taacaaacat
     6541 gtacaacccg aagtctgcat ctgcttctta tcccttttag cagaacaaga atctcctcat
     6601 tttcaggact tgtttttatt tttcgccaat atgttattgc actatcacca atttatgaat
     6661 cccaatgaaa gtgatctgaa tgacgtgttg atgccagcat cattaagtga tgataaaatt
     6721 atcaaacata tggcgcgcag gaccctcaaa ctgtttgtaa aaaatgaaac gccgccaaaa
     6781 gttactcacg aagacctggt gaaaaacagg cctcgctccc ctgtcagacc acctataccc
     6841 gcaaccgcca aaacgccaga cctccctgaa cgtcattaaa ccgtgatgtt accgactctc
     6901 tgactcggaa cagaatcagc gtcagagaaa cggaaaacgc gatccagatc acaaatgcat
     6961 tgtattcaca tcattaaccg ttttaagatc atttcatcac tttttcgcaa ctcacccgat
     7021 aatctgttat gacaacaaac actgtttccc gcaaagtggc gtggctacgg gtcgttacgc
     7081 tggcagtcgc cgccttcatc ttcaacacca ccgaatttgt ccctgttggc ctgctctctg
     7141 acattgcgca aagttttcac atgcaaaccg ctcaggtcgg catcatgttg accatttacg
     7201 catgggtagt agcgctaatg tcattgcctt ttatgttaat gaccagtcag gttgaacggc
     7261 gcaaattact gatctgcctg tttgtggtgt ttattgccag ccacgtactg tcgtttttat
     7321 cgtggagctt taccgttctg gtgatcagtc gcattggtgt ggcttttgca catgcgattt
     7381 tctggtcgat tacggcgtct ctggcgatcc gtatggctcc ggccgggaag cgggcacagg
     7441 ccttgagttt aattgccacc ggtacagcac tggcgatggt cttaggttta ccgctcgggc
     7501 gcattgtggg gcagtatttc ggctggcgaa tgaccttctt cgcgattggt attggggcgc
     7561 ttgttaccct tttgtgcctg attaagttac ttcccttact gcctagtgag cattccggtt
     7621 cattgaaaag cctcccgcta ttgttccgcc gcccggcatt gatgagcatt tatttgttaa
     7681 ctgtggtggt tgtcaccgcc cattacacgg catacagcta tatcgagcct tttgtgcaaa
     7741 acattgcggg attcagcgcc aactttgcca cggcattact gttattactc ggtggtgcgg
     7801 gcattattgg cagcgtgatt ttcggtaaac tgggtaatca atatgcgtct gcgttggtaa
     7861 gtacggcgat tgcgctgttg ctggtgtgtc tggcactgct gctacctgcg gcgaacagtg
     7921 aaatacacct cggggtgctg agtattttct gggggatcgc gatgatgatc atcgggcttg
     7981 gtatgcaggt taaagtgctg gcgctggcac cagatgctac cgacgtcgcg atggcgctat
     8041 tctccggcat atttaatatt ggaatcgggg cgggtgcgtt ggtaggtaat caggtgagtt
     8101 tgcactggtc aatgtcgatg attggttatg tgggcgcggt gcctgctttt gctgcgttaa
     8161 tatggtcaat cattatattt cgccgctggc cagtgacact cgaagaacag acgcaatagt
     8221 tgaaaggccc attcgggcct tttttaatgg tacgttttaa tgatttccag gatgccgtta
     8281 ataataaact gcacacccat acataccagc aggaacccca tcaggcggga gatcgcttca
     8341 atgccaccct tgcccaccag ccgcataatt gcgccggagc tgcgtaggct tccccacaaa
     8401 ataaccgcca ccaggaaaaa gatcagcggc ggcgcaacca tcagtaccca atcagcgaag
     8461 gttgaactct gacgcactgt ggacgccgag ctaataatca tcgctatggt tcccggaccg
     8521 gcggtacttg gcattgccag tggtacaaag gcaatattgg cactgggttc atcttccagc
     8581 tcttccgact tgcttttcgc ctccggtgaa tcaatcgctt tctgttgcgg aaaaagcatc
     8641 cgaaaaccga taaacgcgac gattaagccg cctgcaattc gcagaccggg aatcgaaatg
     8701 ccaaatgtat ccatcaccag ttgcccagcg taatacgcca ccatcatgat ggcaaatacg
     8761 tacaccgagg ccatcaacga ctgacgatta cgttcggcac tgttcatgtt gcctgccagg
     8821 ccaagaaata gcgcgacagt tgttaatggg ttagctaacg gcagcaacac caccagcccc
     8881 aggccaattg ctttaaacaa atctaacatt ggtggttgtt atcctgtgta tctgggttat
     8941 cagcgaaaag tataaggggt aaacaaggat aaagtgtcac tctttagcta accttgcatc
     9001 gcattgacca aaacttgaac cgatttagca aaacgtggca tcggtcaatt catttgactt
     9061 atacttgcct gggcaatatt accccctaca actaattact tgccaaggca actaatgtga
     9121 aaagtaccag cgatctgttc aatgaaatta ttccattggg tcgcttaatc catatggtta
     9181 atcagaagaa agatcgtctg cttaacgagt atctgtctcc gctggatatt accgcggcac
     9241 agtttaaggt gctctgctct atccgctgcg cggcgtgtat tactccggtt gaactgaaaa
     9301 aagtgttgtc ggtcgacctg ggagcactga cccgtatgct ggatcgcctg gtctgtaaag
     9361 gctgggttga aaggttgccg aacccgaatg ataagcgcgg cgtactggta aaacttacca
     9421 ccagcggcgc ggcaatatgc gaacaatgcc atcaattagt tggccaggac ctgcatcaag
     9481 aattaacaaa aaacctgacg gcggacgaag ttgcaacact tgagcatttg cttaagaaag
     9541 tcctgccgta aacaaaaaag aggtatgacg atgtccagac gcaatactga cgctattacc
     9601 attcatagca ttttggactg gatcgaggac aacctggaat cgccactgtc actggagaaa
     9661 gtgtcagagc gttcgggtta ctccaaatgg cacctgcaac ggatgtttaa aaaagaaacc
     9721 ggtcattcat taggccaata catccgcagc cgtaagatga cggaaatcgc gcaaaagctg
     9781 aaggaaagta acgagccgat actctatctg gcagaacgat atggcttcga gtcgcaacaa
     9841 actctgaccc gaaccttcaa aaattacttt gatgttccgc cgcataaata ccggatgacc
     9901 aatatgcagg gcgaatcgcg ctttttacat ccattaaatc attacaacag ctagttgata
     9961 acgtgacaac gtcactgagg caatcatgaa accactttca tccgcaatag cagctgcgct
    10021 tattctcttt tccgcgcaga gcgttgcgga acaaaccacg cagccagttg ttacttcttg
    10081 tgccaatgtc gtggttgttc ccccatcgca ggaacaacca ccgtttgatt taaatcacat
    10141 gggtactggc agtgataagt cggatgcgct cggcgtgccc tattataatc aacacgctat
    10201 gtagtttgtt ctggccccga catctcgggg cttattaact tcccaccttt accgctttac
    10261 gccaccgcaa gccaaataca ttgatataca gcccggtcat aatgagcacc gcacctaaaa
    10321 attgcagacc cgttaagcgt tcatccaaca atagtgccgc acttgccagt ccgactacgg
    10381 gcaccagtaa cgataacggt gcaacccgcc aggtttcata gcgtcccagt aacgtccccc
    10441 agatcccata accaacaatt gtcgccacaa acgccagata catcagcgac aagatggtgg
    10501 tcatatcaat agtaaccaga ctgtgaatca tggatgcgga accatcgaga atcagcgaga
    10561 caacaaagaa gggaatgatt gggattaaag cgctccagat taccagcgac atcaccgccg
    10621 gacgcgttga gtgcgacatg atctttttat tgaagatgtt gccacacgcc caactaaatg
    10681 ctgcagccag ggtcaacata aagccgagca tcgccacatg ctgaccgttc agactatctt
    10741 cgattaacac cagtacgcca aaaatcgcta aggcgatccc cgccaattgt ttgccatgca
    10801 gtcgctcccc gaaagtaaac gcgccaagca cgatagtaaa aaacgcctgt gcctgtaaca
    10861 ccagcgaagc cagtccagca ggcataccaa agttaatggc gcagaacaga aaggcaaact
    10921 gcgcaaaact gatggttaat ccatacccca agagcaaatt cagtggtact ttcggtcgtg
    10981 caacaaaaaa gatggccgga aaagcgacca gcataaagcg caaaccggcc agcatcagcg
    11041 gtggcatgtt atgaagcccc actttgatga ccacaaaatt tagcccccat acgaccacta
    11101 ccagtagcgc caacacccca tcttttcgcg acattctacc gcctctgaat ttcatctttt
    11161 gtaagcaatc aacttagctg aatttacttt tctttaacag ttgattcgtt agtcgccggt
    11221 tacgacggca ttaatgcgca aataagtcgc tatacttcgg atttttgcca tgcgtccgat
    11281 ggttaataac aacgacgagg caagaagggc gctggtagag cgtcgtaggg ataagttcat
    11341 ttgtctgtcc ggcagtgcgt ttcgttatgt tttattttag agatgtaaag aaatagcatg
    11401 gctacgctgc catttatgac catttacttg agtcgccagt acagcctgag tgtcgatcta
    11461 atcggttatg cgatgacaat tgcgctcact attggcgtcg tttttagcct cggttttggt
    11521 atcctggcgg ataagttcga caagaaacgc tatatgttac tggcaattac cgccttcgcc
    11581 agcggtttta ttgccattcc tttagtgaat aacgtgacgc tggttgtgct cttttttgcc
    11641 ctcattaact gcgcctattc tgtttttgct accgtgctga aagcctggtt tgccgacaat
    11701 ctttcgtcca ccagcaaaac gaaaatcttc tcaatcaact acaccatgct aaacattggc
    11761 tggaccatcg gtccgccgct cggcacgctg ttggtgatgc agagcatcaa tctgcccttt
    11821 tggctggcag ctatctgttc cgcgtttccc atgcttttca ttcaaatatg ggtaaagcgc
    11881 agcgagaaaa tcatcgccac ggaaacaggc agtgtctggt cgccgaaagt tttattacaa
    11941 gataaagcac tgttgtggtt tacctgctct ggttttctgg cttcttttgt aagcggcgca
    12001 tttgcttcat gcatttcaca atatgtgatg gtgattgctg atggggattt tgccgaaaag
    12061 gtggtcgcgg ttgttcttcc ggtgaatgct gccatggtgg ttacgttgca atattccgtg
    12121 ggccgccgac ttaacccagc taacatccgc gcgctgatga cagcaggcac cctctgtttc
    12181 gtcatcggac tggtcggttt tattttttcc ggcaacaacc tgctattgtg gggtatgtca
    12241 gctgcggtat ttactgtcgg tgaaatcatt tatgcgccgg gcgagtatat gttgattgac
    12301 catattgcgc cgccaggaat gaaagccagc tatttttccg cccagtcttt aggctggctt
    12361 ggtgccgcga ttaacccatt agtgagtggc gtagtgctaa ccagcctgcc gccttcctcg
    12421 ctgtttgtca tcttagcgtt ggtgatcatt gctgcgtggg tgctgatgtt aaaagggatt
    12481 cgtgcaagac cgtgggggca gcccgcgctt tgttgattta agtcgaacac aataaaaatt
    12541 taattcagcc ttcgtttagg ttatctctgc taatatcttt ctcattgaga tgaaaattaa
    12601 ggtaagcgag gaaacacacc acaccataaa cggaggcaaa taatgctggg taatatgaat
    12661 gtttttatgg ccgtactggg aataatttta ttttctggtt ttctggccgc gtatttcagc
    12721 cacaaatggg atgactaatg aacggagata atccctcacc taaccggccc cttgttacag
    12781 ttgtgtacaa ggggcctgat ttttatgacg gcgaaaaaaa ccgccagtaa accggcggtg
    12841 aatgcttgca tggatagatt tgtgttttgc ttttacgcta acaggcattt tcctgcactg
    12901 ataacggatc gttgacacag tagcatcagt tttctcaatg
//
LOCUS       DAZSNM010000115        12537 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZSNM010000115 DAZSNM010000000
VERSION     DAZSNM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12537
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.115"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            181..1314
                     /gene="wzz(fepE)"
                     /locus_tag="ACHW42_002643"
                     /pseudo
     CDS             181..1314
                     /gene="wzz(fepE)"
                     /locus_tag="ACHW42_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
     gene            complement(1311..2126)
                     /gene="fepC"
                     /locus_tag="ACHW42_002644"
     CDS             complement(1311..2126)
                     /gene="fepC"
                     /locus_tag="ACHW42_002644"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR8944406.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(2123..3115)
                     /gene="fepG"
                     /locus_tag="ACHW42_002645"
     CDS             complement(2123..3115)
                     /gene="fepG"
                     /locus_tag="ACHW42_002645"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR8944407.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(3112..4116)
                     /gene="fepD"
                     /locus_tag="ACHW42_002646"
     CDS             complement(3112..4116)
                     /gene="fepD"
                     /locus_tag="ACHW42_002646"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR8944408.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            4227..5459
                     /gene="entS"
                     /locus_tag="ACHW42_002647"
     CDS             4227..5459
                     /gene="entS"
                     /locus_tag="ACHW42_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR8944409.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGTAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            complement(5463..6419)
                     /gene="fepB"
                     /locus_tag="ACHW42_002648"
     CDS             complement(5463..6419)
                     /gene="fepB"
                     /locus_tag="ACHW42_002648"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR8944410.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            6711..7886
                     /gene="entC"
                     /locus_tag="ACHW42_002649"
                     /pseudo
     CDS             6711..7886
                     /gene="entC"
                     /locus_tag="ACHW42_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            7896..9506
                     /gene="entE"
                     /locus_tag="ACHW42_002650"
     CDS             7896..9506
                     /gene="entE"
                     /locus_tag="ACHW42_002650"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR8944411.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            9520..10377
                     /gene="entB"
                     /locus_tag="ACHW42_002651"
     CDS             9520..10377
                     /gene="entB"
                     /locus_tag="ACHW42_002651"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR8944412.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            10377..11123
                     /gene="entA"
                     /locus_tag="ACHW42_002652"
     CDS             10377..11123
                     /gene="entA"
                     /locus_tag="ACHW42_002652"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR8944413.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            11126..11539
                     /gene="entH"
                     /locus_tag="ACHW42_002653"
     CDS             11126..11539
                     /gene="entH"
                     /locus_tag="ACHW42_002653"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR8944414.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            11720..>12537
                     /locus_tag="ACHW42_002654"
     CDS             11720..>12537
                     /locus_tag="ACHW42_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFR8944415.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPV"
BASE COUNT         2808 a         3258 c         3591 g         2880 t
ORIGIN      
        1 taattatggg ttttttattt gtttgattta taggtttgat gaatatttct cttaaataga
       61 gtgaatgttg cattcagtgg aaggaattta caaacttcag cttcggataa ttctcaccaa
      121 ctggtaaagt gagcgccgtg gagattttcc cctattggac tcattttcag gttatacccc
      181 atgtcatcat tgaatattaa acagggaagt gacgctcatt tttctgagta tcctctggcg
      241 tcgcccagta ataatgaaat tgatttactt aatctaatcg aagttttatg gcgggccaaa
      301 aaaacggtca tggcggtcgt ttttgcattt gcctgcgcag gtttactgat ctctttcatc
      361 ctgccgcaaa aatggaccag ctcagccgtt attactcccg ccgaagccat tcagtggcag
      421 gacctggaga aaacctttac caaactccgc gttcttgatc tcgatgttaa tatcgaccgt
      481 ggcggtgcat ttaacctgtt tatcaagaag tttcagtctg ttagcttgct ggaagagtac
      541 ctgcgttcat caccttatgt gatggactaa ttaaaagagg cgaaaatcga tgaactggat
      601 ttgcatcgcg ctattgttgc attgagcgaa aaaatgaaag cggttgatga caatgctagt
      661 aagaaaaaag atgaaccgtc actgtataca tcctggacgc taagttttac cgcgccaacc
      721 agtaaagagg cgcagaccgt tttgagcggg tatatcgatt atatctctgc gttggtggtg
      781 aaagagtcga tagaaaacgt ccgtaataaa ctggagatca aaacccagtt tgaaaaagaa
      841 aaactggctc aggatcgcat taaaacgaaa aatcaacttg atgcaaacat tcagcgcctc
      901 aattattcac tcgacattgc caacgcggca ggaattaaaa agcccgtata cagtaatggt
      961 caggccgtta aagatgaccc cgatttctct atttccctcg gcgcagacgg tattgaacgc
     1021 aaactggaaa ttgaaaaagc ggttactgac gttgcggaac tgaacggtga attacgtaat
     1081 cggcagtatc ttgtcgagca attaacaaaa gcaaatgtca acgatgtgaa ttttacgccg
     1141 tttaaatatc agttaagccc gtcattgcca gtgaaaaaag acggtccggg taaggcgatt
     1201 attgtgatcc tttccgcgct gatcggcggg atggtggctt gtggtagcgt gttattgcgc
     1261 tatgcgatgg catccagaaa acaggatgcc atgatggcag accacttagt ttaactattt
     1321 gcggttgaag gtgccgttcg tccgagcggc accacaagcg gcattccggc taccggatcg
     1381 tcaatgatca tgcatcgcag accataaatg cgctcaatca gttcagcagt gacaatctcc
     1441 ttcggcgctc cctgagcaac aattttccct tcccgcaatg caatcaaatg gctggcgtaa
     1501 cgacaggcct gattaagatc gtgcagcacc gccgccaggg tatagccttt ctcgcggttc
     1561 agttcgctta acagttccag tagatcaatc tgatgactga tatccagcca ggtcgtgggt
     1621 tcgtcgagca gcataattgc cgtttcctgg gccagcacca tcgcgatcca cgctcgctgg
     1681 cgttgtccgc cagaaagggt atccacgctt tgatctgcca gatgagttat tcccgtggcc
     1741 tgcatcgctt tcgttaccgc ttcttcatcc tctttgcgcc agcgggtaaa cagcggttga
     1801 tgcggataac gtccacgcgc caccagctcc tgcacggtga tatcgcccgg cgtggtagca
     1861 ttttgcgcca acagaccaat ccggcgtgca acttctttac tggcgtaatg ttgaatgtgc
     1921 tcgccatcca gccagacatg cccgtgagca ggcgtcatca ggcggcttaa ggtacgcagt
     1981 aacgtggatt taccacagcc atttggtccg ataattgccg tgaagtgacc atcaggaatt
     2041 tctacagtca gattttccgc aacggtatat ttgccatatc ccagggttaa ctgttcgccg
     2101 cgcaaacggg ctactgattc ggtcattttt tgcgagactc ctgaattaac aagacgataa
     2161 ggtaaatacc gccgaggctg acggtaacga cgccaaccgg aagttgatac ggcataaaca
     2221 gttgttgggc gcagagaacg gccgccagca gtaacagcgc tccgcacagc gccgcctggg
     2281 tcagccccca gcgagcggtg ccgctaatgc gtcgggcaat gtgcggcgcg actaaagcaa
     2341 taaaggaaat cggcccggca agcgccgttg ccgcagcggt aagcaccact gcaaccagca
     2401 tcattaacag acgcgaacgc tcgacgctga cgcccagcgc gcaggcggta tcatcgccca
     2461 tctccagcaa gcgcattcgc cgcaccagca aggtggcggc aatgagcatc aatataatga
     2521 tgggtgcgga aggcgaggtt tttgcccacg tcaggccgtt gagcgatccg gcattccaca
     2581 aacctgctgt tagcgccgtt tctaaagacg ctttcagcaa cagccaggta ttaaaggcca
     2641 ccagcatggc gcgaacgccg ataccgataa taatcaaccg aaaggtgtcg ataccgttac
     2701 gccaggcgag cagccagacc aacagcgaag tgataatgcc gcccaccatt gctgccagcg
     2761 cgatagccgt cagatcctga ccaaacagca ccatcgccac cagcacgccg ctccacgccc
     2821 cggtgttaaa gcccattacg tcagggctgc cgagcgggtt acgcatcagc gactgaaaaa
     2881 tcgcgccgct gacgcccagc gccgcgccaa tcaacagcgc catcagcacg cgtggtaaac
     2941 gccattcggt gaccaccatc gtcatactgc gcggcgcatc gcccatcaac gcggcgaata
     3001 cctgcgagat ttccagcgtg acggcaccgc tgcgtaatcc ccagatacct gcaaccacac
     3061 aggcggaaat cagcagcaaa caggtgatga gtaatcggcg agagacgtaa atcatgcacc
     3121 acctcgcgtt ttacgtcgca cgaggaagat cagcaccggt gcaccaataa acgcactgac
     3181 cacagaaacg cgcagttcgc cgggcacaat cacccgcccg atgatatcgg caaacagcag
     3241 cagggcaggg gtagcaagta gcgtgacggg cagcgaccag cgatgatcgg cacctaccag
     3301 ccagcgcgcc atgtgcggca tcatcaggcc gataaaggca atcgggccaa ctaccgccgt
     3361 cgcactacca caaagcacgg taatcgccag cagaccaatc aactgtgtgc gcgccacgcg
     3421 actgcccagc gccgtcgcgg tgtcactgcc gaggctcaaa ctgttcagcg cgcgactcag
     3481 taatagcgca gttgctccgg cgatcagaac cgggatcagc accactttta aggtatgtag
     3541 attgcgaata tccagcgaac cggcttgcca gaaacgcagt tgatcgtaga cgtcaggatt
     3601 aagcagggcg atgccgctgg tcagtccttc cagcaccgcc gccagcgcca cgcccgccag
     3661 ggttaaacgc accggactta actgccctcc accctgactg ccggtaaagg caacaatcaa
     3721 tgaggccacc agcgccccgg cgaaggccat cgccagttgt tcctgtgcgg atgagtaacc
     3781 aaacagcgcc gcacccagca caatggcaaa gctggctccg gcgttcacgc caagcaagcc
     3841 ggggtcggca agtgggtttc gggtgagggt ttgcattaac gccccggcaa ggccaagcgc
     3901 gccgcctgcc agtaaaccgg caagggtacg cggcagccgc gcgtcgagca cgatggtgca
     3961 gtcggcgctc tggcaggtgc cggagaaggc ctccagcact acggaagcgg ggagtgattt
     4021 tgccccaatg agcaggctta atgccgtcgc gataatcagt aataacagca atccgggcac
     4081 ggcaatggcg cgtgtcacgg caacagaacc agatatacga acttccatga taatgaaatt
     4141 aattatcgtt atcgatctta tttggatatg ttaacatgtg cagcctaaga ataggtattt
     4201 aaaatatttg atggcaaggc attgtaatga ataaacaatc ctggctgctt aacctcagcc
     4261 tgttgaaaac gcacccggcg tttcgcgcag tattcctcgc tcgttttatc tcaattgtgt
     4321 ctctgggttt gctcggcgtc gcggtgccgg tgcagatcca gatgatgacc cattctacct
     4381 ggcaggtggg gctttcggtg acgctgaccg gcggcgcgat gtttgttggc ctgatggttg
     4441 gcggtgtgct ggcggatcgc tatgaacgca aaaaagtgat tttgctggcg cgcggcacct
     4501 gtggcattgg cttcattgga ctgtgcctta atgcactgct gccggagccg tcattgctgg
     4561 caatctattt acttggttta tgggatggtt ttttcgcatc acttggtgtt acggcgctac
     4621 tggcggcaac atctgcactg gtagggcgtg aaaacttaat gcaggccggg gcgatcacca
     4681 tgttgaccgt gcgcctgggg tcggtgattt cgcccatgat tggcggttta ttgctggcga
     4741 ccggcggcgt agcctggaac tacgggctgg cggcggcggg cacgtttatt accttgttac
     4801 cgttattaag ccttccggag ttgccaccgc caccgcagcc gcttgagcat ccgttgaaat
     4861 cattactggc aggatttcgt tttctgcttg ccagcccgct gctgggtggt ttattgacga
     4921 tggcgagcgc ggtgctggta ctgtatccgg cgctggctga caactggcag atgtcagcgg
     4981 cacagattgg ttttctctac gcggcgatcc cgctcggcac ggctattggt gcgttaacca
     5041 gcgggaagct ggcacatagt gcgcgaccag ggttattgat gctgctctcc acgctgggat
     5101 cgttcctcgc cattggtctg tttggcctga tgccgatgtg gattttaggc gtggtttgtc
     5161 tggcgctgtt cggctggctg agcgcggtta gttcgttgct gcaatataca atgctgcaaa
     5221 cgcaaacccc ggaagcgatg ttagggcgga ttaacggttt gtggacggcg cagaacgtga
     5281 cgggcgatgc cataggcgcg gcgctgctgg gtggtttggg cgcgatgatg acaccggttg
     5341 cttccgcaag cgcgagcggt tttggtttgt tgattatcgg cgtgttgtta ttgctggtgc
     5401 tggtggagtt gcgacgtttt cgccagacgc cgccgcaggt gacagcgtcc gacagttaat
     5461 gcttaaaaca gcgccttaag ccgttccagc acttgcatgg cgctgtagta atccaggcgg
     5521 aacgtctcgg ttcccagcgc ataaacctgc ttgttttgta ccgcaggcag gtgcgcgagc
     5581 agtggattgg cataaatcgc atcggcatct ttctgatcac cagcgaacag gaatagcgat
     5641 tcaccgttta accccgcagc cagattttcc ccaccaagct gaatgatgtc atggcgtttg
     5701 ccctggcttt ggctggcgtt taaccctgcg ggcaatttcg ccagcgtaaa gccgagttgt
     5761 tccagcatct gcccttgtgc tgattccggc gtccagagat tggcactgtg tgcagcggcg
     5821 gtatagacaa tggcagtgac cggctgcggc ggtaatttga tttgctcttt cgccgctgcc
     5881 agttgcttat caaactgtgc aatccgctct gccgcttgtt tctcatgccc ggtaatttcg
     5941 ccaagctgcg ttaacagcga ctgccagctt ttgtcgtcgt aattgatgat taatgtcggg
     6001 gcgatggtgg aaagctgatc atacagcgcc agcgccgaat ccccgccggt tgcgctaatt
     6061 aaaatcagat ccggcatttg cgcggcaacg gcttcggcgc tcggttcgcc gatatagagc
     6121 cgttgcagtt tgcgttcttt cgccaccttg ctccactggc gtaaaaagcc ctggtcatct
     6181 gcgacgcggt tattcggcgt ggtcgcgccg ctggcgatca ccggagcatc aatcgccagc
     6241 agtgatccgg tcagggtgac gctggtggaa acaatacgct gcggctggct ttccagggta
     6301 tgagtgcccc ggctgtcagt aatctgacgc ggccagtcgg cggcctgaac tacggctatt
     6361 cctgaaagca aaagtcctgt taatagaagg gcgttgcggt agagcggggc gagtctcaca
     6421 agtcagcttc ctgttattaa taaggttaag ggcgtaatga caaattcgac aaagcgcaca
     6481 atccgtccct ttggggagag ggggcaaaac ggcgcaggac atcacattgc gcttatgcga
     6541 attcatcaat aatgcttctc attttcattg taaccacaac cagatgcaac cccgagttgc
     6601 agattgcgtt acctcaagag ttgacatagt gcgcgtttgc ttttaggtta gcggccgaaa
     6661 atataagtga taatcattat taaagccttt atcattttgt ggaggatgat atggatacgt
     6721 cactggctga ggaagtacag cagaccatgg caacacttgc gcccaatcgc tttttcttta
     6781 tgtcgccgta ccgcagtttt acgacgtcag gatgtttcgc ccgtttcgat gaaccggctg
     6841 tgaacgggga ttcgcccgac agtcccttcc agcaaaaact cgccgcgctg tttgccgatg
     6901 ccaaagcgca gggcatcaaa aatccggtga tggtcggggc gattcccttc gatccacgtc
     6961 agccttcgtc gctgtatatt cccgaatcct agcagtcgtt ctcccgtcag gaaaaacaga
     7021 cctcagcccg ctgttttacc cgcagccagt cgctgaacgt ggtggaacgc taggcaattc
     7081 ctgaacaaac cacgtttgaa cagatgggtg cccgcgccgc cgcacttacc gccacgccgc
     7141 aggtcgacaa agtggtgttg tcacgattga ttgatatcac cactgacgcc gccattgata
     7201 gtggcgtatt gctggaacgg ttgattgcgc aaaacccggt tagttacaac ttccatgtcc
     7261 cgctggctga tggtggcgtc ctgctggggg ccagcccgga actgctgcta cgtaaagacg
     7321 gcgagcgttt tagctccatt ccgttagccg gttccgcgcg tcgtcagccg gatgaagtgc
     7381 tcgatcgcga agcgggtaat cgtctgctgg cgtcagaaaa agatcgccat gaacatgaac
     7441 tggggactca ggcgatgaaa gaggtactgc gcgaacgcag tagtgagtta cacgttccct
     7501 cctctccaca attgattacc acgccgacgc tgtggcatct cgcaactccc tttgaaggta
     7561 aagcgaattc gcaagaaaac gcactgactc tggcctgtct gctgcatccg acccccgcgc
     7621 tgagcggttt cccgcatcag gccgcgaccc aggttattgc tgaactggag ccgttcgacc
     7681 gcgaactgtt tggcggcatt gtgggttggt gtgacagcga aggtaacggc gaatgggtgg
     7741 tgaccatccg ctgcgcgaag ctgcgggaaa atcaggtgcg tctgtttgcc ggagcgggga
     7801 ttgtgcctgc gtcgtcaccg ttgggtgagt ggcgcgaaac aggcgtcaaa ctttctacca
     7861 tgttgaacgt ttttggattg cattaaggag cgaggatgag cattccattc acccgctggc
     7921 cggaagagtt tgcccgtcgc tatcgggaaa aaggctactg gcaggatttg ccactgaccg
     7981 acattctgac tcgccacgct gcgagtgaca gcatcgcggt tatcgacggc gagcggcagt
     8041 tgagttatcg ggagctgaat caggcggcgg ataacctcgc ctgtagtttg cgccgtcagg
     8101 gcattaaacc tggtgaaacc gcgctggtac aactgggtaa cgtcgctgaa ctttacatta
     8161 cctttttcgc gctgctgaaa ctgggcgtcg cgccggtgct ggcgctgttc agccatcagc
     8221 gtagtgaact gaacgcctac gccagccaga ttgaaccggc gttactgatt gccgatcgcc
     8281 agcatgcgct atttagcggg gatgatttcc tcaacacatt tgttgcagag cattcttcca
     8341 ttcgcgtggt gcaactgcac aacgacagcg gtgagcataa cttgcaggat gcgattaacc
     8401 atccggcaga gggttttact gccacgccat cacctgctga tgaagtggcc tatttccagc
     8461 tttccggcgg caccaccggc acaccgaaac tgatcccgcg cactcataac gactattact
     8521 acagcgtgcg tcgtagcgcc gagatttgtc agttcacaca acagacgcgc tacctgtgct
     8581 cgatcccggc ggctcataac tacactatga gttcgccagg atcgctgggc gtctttcttg
     8641 ccggaggaac ggttgttctg gcggccgatc ccagcgccac gctttgcttc ccgttgattg
     8701 caaaacatca ggttaacgtt accgcgctgg tgccgccggc agtcagcctg tggttgcagg
     8761 cgctggctga aggcgaaagc cgggcgcagc ttgcctcgct gaaactgtta caggtcggcg
     8821 gcgcacgtct ttctgccacg cttgcagcgc gtattcccgc tgagattggc tgccagttgc
     8881 agcaggtgtt tggcatggcg gaagggctgg tgaactacac ccgtcttgat gatagcgcgg
     8941 agaaaattat ccatacccag ggttacccaa tgtgtccgga cgatgaagtg tgggttgccg
     9001 atgccgaagg aaatccactg ccgcaagggg aagttggacg cctgatgacg cgcgggccgt
     9061 acaccttccg tggctattac aaaagtccgc agcacaatgc cagtgccttt gatgccaacg
     9121 gtttttactg ttccggcgat ctgatctcta ttgatccaga gggttacatc accgtgcagg
     9181 ggcgcgagaa agatcagatc aaccgtggcg gcgagaagat cgctgccgaa gatatcgaaa
     9241 acctgctgct gagccatccg gcagtgatct atgccgcact ggtgagcatg gaaaatgagc
     9301 tgatgggcga aaaaagctgt gcttatctgg tggtaaaaga gccgctgcgc gcggtgcagg
     9361 tgcgtcgttt cctgcgtgaa cagggtattg ccgaatttaa attaccggat cgcgtggagt
     9421 gtgtggattc acttccgctg acggcggtcg ggaaagtcga taaaaaacaa ttacgtcagt
     9481 ggctggcgtc acgcgcatca gcctgaagga gagaacacga tggctattcc aaaattacag
     9541 gcttacgcac tgccggagtc tcacgatatt ccgcagaata aagttgactg ggcctttgaa
     9601 ccgcaacgtg ccgcgttgtt aatccatgat atgcaggact attttgtcag cttctggggc
     9661 gagaactgcc cgatgatgga gcaggtgatc gcgaatattg ctgcgctgcg cgactactgc
     9721 aaacagcaca atatcccggt ttattacacc gcccagccga aagagcagag cgatgaagat
     9781 cgggcgctgt tgaatgatat gtgggggccg ggcctgaccc gctcgccgga acagcaaaag
     9841 gtggtggatc gcctgacgcc agatgccgac gacacggtgc tggtgaagtg gcgctacagc
     9901 gcgtttcatc gttctccgct ggagcaaatg ctcaaagaga gtggacgtaa ccagctgatt
     9961 attacaggag tatatgccca cattggctgt atgaccaccg caaccgacgc atttatgcgc
    10021 gatattaaac cgtttatggt ggcggatgcg ctggctgatt tcagccgtga cgagcatttg
    10081 atgtcgctga aatatgtggc cggacgttct ggccgggtgg tgatgaccga agaattactg
    10141 ccagcaccta tccccgccag caaagcggcg ctgcgtgagg tgatcctgcc gttgctggac
    10201 gagtccgatg aaccgttcga tgacgacaac ctgatcgact acggtctgga ttcggtgcgc
    10261 atgatggcgc tggcggcgcg ctggcgcaaa gtgcatggtg atatcgactt tgtcatgctg
    10321 gcgaaaaacc cgaccatcga cgcctggtgg aagctactct cccgcgaggt gaaataatgg
    10381 atttcagcgg taaaaatgtc tgggtaaccg gcgcaggtaa aggtatcggc tacgccacgg
    10441 cgctgacgtt tgttgaggcg ggagcgaaag ttacaggttt tgatcaagcg ttcactcagg
    10501 agcaatatcc ctttgcgacc gaagtaatgg atgttgccga cgccgggcag gtggcgcagg
    10561 tatgtcagcg actgttagct gaaacggagc gactggacgt gctgatcaat gcggcgggaa
    10621 ttttgcgcat gggcgcgacc gatcagctca gtaaagagga ctggcagcag acttttgcgg
    10681 ttaacgtcgg cggtgcgttt aacctgttcc agcaaaccat gaaccagttt cgccgtcagc
    10741 ggggcggggc gattgtcact gtggcgtccg acgccgcaca cacggcgcgt attggtatga
    10801 gtgcttatgg cgcatcgaaa gcggcgctga aaagcctggc gttgagcgtc gggctggaac
    10861 tggcgggtag cggcgtgcgc tgtaatgtgg tttcgcctgg ctccaccgac accgatatgc
    10921 aacgcacgct gtgggtgagc gatgacgccg aagaacagcg tattcgcggc tttggcgagc
    10981 agtttaaact cggcattccg ctggggaaaa tcgcccgtcc acaagagatc gccaacacga
    11041 ttttgttcct cgcctctgac ctcgccagcc atatcaccct acaggatatt gtggtcgatg
    11101 gcggctcaac gctgggggca taagcatgat ctggaaacgc catttaacgc tcgacgaact
    11161 gaacgccacc agcgataaca caatggtggc gcatctggga attgtgtata cccttctggg
    11221 agatgatgtg ctggaagccg aaatgccggt tgatacccgt actcatcagc cgtttggcct
    11281 gctgcatggc ggcgcgtcgg cggcgctggc tgaaacgctg ggatcgatgg ccggatttat
    11341 gatgacccgt gacgggcagt gtgtggtggg cacggaactt aacgccaccc atcatcgccc
    11401 ggtgtctgaa ggcaaggtac gcggcgtctg ccagccgctg catcttggga ggcaaaatca
    11461 gagttgggaa atcgtcgttt tcgatgaaca ggggcggcgt tgctgcactt gtcggctggg
    11521 tacggcagtt ttgggatgag cccgacagaa ttaaatgaga ttgcaggaaa actcggttaa
    11581 cggagtgatc gagttaacat tgttaagtta aatattggtt tcaactccga tttacatggt
    11641 taccgtgttg ttaaatcgta caaagatgtt atagaaacaa aatgtaacat ctctatggac
    11701 acgcacacgg ataacaacta tgaacaaatc agggaaatac ctcgtctgga cagtgctctc
    11761 tgtaatggga gcatttgctc tgggatacat tgctttaaat cgtggggaac agatcaacgc
    11821 gctgtggatt gtggtggcgt cggtctgtat ctatctgatc gcttaccgtt tttatgggct
    11881 gtatatcgcc aaaaatgtgc tggcggttga cccgacgcgt atgacgccag cggtgcgcca
    11941 taacgacggg ctggactatg tgccgacgga caagaaagtg ctgttcggtc accattttgc
    12001 ggccattgcc ggagcagggc cgctggtggg gccggtgctg gcagcgcaaa tgggctacct
    12061 gccggggatg atctggctgc tcgccggggt ggttctcgcc ggtgcggtgc aggatttcat
    12121 ggtgctgttt gtttctacgc gccgtgacgg tcgctcgctg ggtgagctgg tcaaagaaga
    12181 gatggggcca actgccgggg tgattgcgct ggtagcctgc tttatgatca tggtcattat
    12241 ccttgcagta ctggcgatga tcgtggtgaa agccctgact catagcccgt ggggaacgta
    12301 taccgttgcg ttcaccattc cgctggcgat ttttatggga atctacctgc gctatctgcg
    12361 tccgggccgc attggcgaag tgtcagtgat tggcctggtg ttcctgattt tcgccattat
    12421 ctctggcggc tgggtggcag aaagtccgac ctgggcaccg tactttgact ttaccggcgt
    12481 gcagctgacc tggatgctgg tgggctacgg ttttgtggcg gcggtgctgc cggtgtg
//
LOCUS       DAZSNM010000116        12510 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZSNM010000116 DAZSNM010000000
VERSION     DAZSNM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12510
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.116"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACHW42_002655"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACHW42_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR8944416.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     misc_feature    547..670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="ACHW42_002656"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="ACHW42_002656"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR8944417.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="ACHW42_002657"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="ACHW42_002657"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR8944418.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="ACHW42_002658"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="ACHW42_002658"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR8944419.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="ACHW42_002659"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="ACHW42_002659"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR8944420.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="ACHW42_002660"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="ACHW42_002660"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR8944421.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="ACHW42_002661"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="ACHW42_002661"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR8944422.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="ACHW42_002662"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="ACHW42_002662"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR8944423.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /gene="hisIE"
                     /locus_tag="ACHW42_002663"
     CDS             7139..7750
                     /gene="hisIE"
                     /locus_tag="ACHW42_002663"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR8944424.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="ACHW42_002664"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="ACHW42_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR8944425.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="ACHW42_002665"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="ACHW42_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="ACHW42_002666"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="ACHW42_002666"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR8944426.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..12258)
                     /locus_tag="ACHW42_002667"
     CDS             complement(11944..12258)
                     /locus_tag="ACHW42_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944427.1"
                     /translation="MKKASEYSELYGDNLIQLGLEDGIYFYKGMAIGDVFGLARYSDW
                     TISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYPKFKVLMDTPNGILIA
                     IK"
BASE COUNT         3187 a         3159 c         3107 g         3057 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggatcaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgagatatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
    12301 caagtattca gaataaaaat atttgaattc aaaatacaac gatcgccccg aaaaaaaata
    12361 atacacacaa ggataaaata tcgtcccaac ttgtaatttg taatgttgat atcttttatt
    12421 aaacttgcaa ttgagatcgc aacctgcaat actgacaatg ggtaaatggg tgataaatac
    12481 ctgtcacctt gcgtaacaga aaaccaaaat
//
LOCUS       DAZSNM010000117        12257 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZSNM010000117 DAZSNM010000000
VERSION     DAZSNM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12257
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.117"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..206)
                     /locus_tag="ACHW42_002668"
     CDS             complement(<1..206)
                     /locus_tag="ACHW42_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR8944428.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIG"
     gene            complement(199..1194)
                     /gene="hlyD"
                     /locus_tag="ACHW42_002669"
     CDS             complement(199..1194)
                     /gene="hlyD"
                     /locus_tag="ACHW42_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR8944429.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(1197..1868)
                     /gene="cecR"
                     /locus_tag="ACHW42_002670"
     CDS             complement(1197..1868)
                     /gene="cecR"
                     /locus_tag="ACHW42_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR8944430.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2097..3317
                     /gene="rhlE"
                     /locus_tag="ACHW42_002671"
     CDS             2097..3317
                     /gene="rhlE"
                     /locus_tag="ACHW42_002671"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR8944431.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSVAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGIRLMNKRKTA"
     gene            complement(3311..3409)
                     /locus_tag="ACHW42_002672"
                     /pseudo
     CDS             complement(3311..3409)
                     /locus_tag="ACHW42_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3491..3973)
                     /gene="ybiA"
                     /locus_tag="ACHW42_002673"
     CDS             complement(3491..3973)
                     /gene="ybiA"
                     /locus_tag="ACHW42_002673"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR8944432.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            4093..6244
                     /gene="dinG"
                     /locus_tag="ACHW42_002674"
                     /pseudo
     CDS             4093..6244
                     /gene="dinG"
                     /locus_tag="ACHW42_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
     gene            6272..7234
                     /gene="ybiB"
                     /locus_tag="ACHW42_002675"
     CDS             6272..7234
                     /gene="ybiB"
                     /locus_tag="ACHW42_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR8944433.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            7375..8460
                     /gene="hcxB"
                     /locus_tag="ACHW42_002676"
     CDS             7375..8460
                     /gene="hcxB"
                     /locus_tag="ACHW42_002676"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR8944434.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            8527..8586
                     /gene="ybiE"
                     /locus_tag="ACHW42_002677"
     CDS             8527..8586
                     /gene="ybiE"
                     /locus_tag="ACHW42_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR8944435.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(8689..8949)
                     /gene="ybiJ"
                     /locus_tag="ACHW42_002678"
     CDS             complement(8689..8949)
                     /gene="ybiJ"
                     /locus_tag="ACHW42_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR8944436.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(9214..9480)
                     /gene="ybiI"
                     /locus_tag="ACHW42_002679"
     CDS             complement(9214..9480)
                     /gene="ybiI"
                     /locus_tag="ACHW42_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR8944437.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(9554..10231)
                     /gene="ybiX"
                     /locus_tag="ACHW42_002680"
     CDS             complement(9554..10231)
                     /gene="ybiX"
                     /locus_tag="ACHW42_002680"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR8944438.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(10273..12257)
                     /locus_tag="ACHW42_002681"
                     /pseudo
     CDS             complement(10273..12257)
                     /locus_tag="ACHW42_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         2796 a         3179 c         3352 g         2930 t
ORIGIN      
        1 aagccaatca ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc
       61 agtgtggttt tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg
      121 gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg
      181 ttcagcgtga taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg
      241 cattccctgg cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag
      301 gtcggtacgc agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac
      361 gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt
      421 gcgccccggc tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg
      481 acgcgttagt gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac
      541 cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag
      601 ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc
      661 ctgttcacgg ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc
      721 ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct
      781 tttccacagt ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc
      841 ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag
      901 atcatactgc gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata
      961 cggcttgtga tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac
     1021 cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac
     1081 gttgccatac agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc
     1141 aaccacggcg gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac
     1201 aaactccttt gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg
     1261 atcagttcgg tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct
     1321 ttgccaagac ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg
     1381 gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc
     1441 ggactaatca cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg
     1501 gagataaact tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg
     1561 caggcgcgaa ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag
     1621 cgttcggctt cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg
     1681 gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc
     1741 tgcccggcct gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc
     1801 gccagtgcgg cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga
     1861 ttattcatcg cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg
     1921 gagttgtcca gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct
     1981 gcgtcacaaa aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct
     2041 tttcagtatc tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt
     2101 ctttcgattc tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc
     2161 gtgaacccac ccctattcag cagcaggcga tccctgctgt gctggaaggc cgcgacctga
     2221 tggctagcgc ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac
     2281 acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta
     2341 ccccgacccg tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc
     2401 tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt
     2461 tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc
     2521 aaaatgcagt gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc
     2581 tcgacatggg ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc
     2641 agaacctgct gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt
     2701 tgcacaaccc gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc
     2761 agcacgttca ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta
     2821 aagggaactg gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg
     2881 ctgagcagct caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag
     2941 gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga
     3001 ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aactatgaac
     3061 tgccaaacgt accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta
     3121 ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa
     3181 aactgctgaa aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa
     3241 tcaaagccga accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca
     3301 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg
     3361 aatttgcatg cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca
     3421 agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc
     3481 caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca
     3541 gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct
     3601 caaccagttt tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga
     3661 atttagcgcg aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa
     3721 gtggcttgga acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga
     3781 tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg
     3841 gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt
     3901 catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg
     3961 ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca
     4021 gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt
     4081 tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag
     4141 gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg
     4201 gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc
     4261 ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa
     4321 aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat
     4381 ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg
     4441 cgctacgttt gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat
     4501 ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt
     4561 gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat
     4621 atcgctatag atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac
     4681 cgtaactgtt actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa
     4741 gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta
     4801 ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg
     4861 gcccgggatg cgctggagat gagcgctgaa atcaccgtgc cctggtatcg actacagctg
     4921 gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca
     4981 ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc
     5041 tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt
     5101 gcgatgggcg aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc
     5161 gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc
     5221 cacgatattg tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt
     5281 gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg
     5341 accaaatggg cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg
     5401 ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt
     5461 gtcacctccg caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt
     5521 ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccccttta accactgcga
     5581 acagggcaaa attgttattc cccggatgcg ctttgagcct tccatcgaca acgaagagca
     5641 acacattgcc gaaatggcgg cctttttccg tgaacaggtg gagagcaaaa aatatctcgg
     5701 tatgttggta ctgtttgcca gcggacgggc gatgcagcgc tttctcgact atgtgacgga
     5761 tttacgcctg atgttgctgg tgcagggcga tcagccgcgt tatcgcttag ttgaactgca
     5821 ccgcaaacgc gttgccaacg gtgagcgtag cgtgctggtg ggcttacagt catttgccga
     5881 agggcttgat ttgaaaggtg atctgctcag ccaggtgcat atccacaaaa tcgcttttcc
     5941 gcccatcgac agcccggtgg tgatcaccga aggggaatgg ctgaaaagcc tcaaccgcta
     6001 tccgtttgag gtgcaaagcc tgccgagcgc ctcgtttaac ctgattcagc aggttgggcg
     6061 actgattcga agccacggtt gctggggcga agtggttatc tacgacaaac gcttgctgac
     6121 aaaaaattat ggcaagcgac tactggatgc attaccggta tttccgatag agcaaccgga
     6181 agtccctgaa ggtatagtta aaaagaaaga aaaaacgaaa tccccacgcc gtcggcggcg
     6241 ttaatgatgt gagtcaggta aggagtcgta aatggactat cgcaaaatca ttaaagagat
     6301 cgggcgcggg aaaaaccacg cgcgcgattt agaccgggat actgcgcgcg gtctgtatgc
     6361 tcatatgctc aatggtgaag tccctgacct tgagttgggc ggcgtattga ttgcgctgcg
     6421 tatcaaaggg gaaggggaag cagagatgct cggcttttac gaagccatgc aaaatcacac
     6481 catcaagctg acgccgcctg caggcaagcc gatgccgatt gtcatcccca gttacaacgg
     6541 cgcgcgtaaa caggctaacc tgacgccatt attggcgatt ctgctgcata aactcggttt
     6601 tcctgtggtg gttcacggag ttagcgaaga tccaactcgc gtgctgacag aaaccatttt
     6661 cgaactgatg ggtattacac caacgttgca cggcgggcag gcgcaggcga agctcgacga
     6721 acatcaaccg gtgtttatgc ctgtcggcgc gttttgcccg ccgctggaaa aacaactggc
     6781 gatgcgctgg cggatgggcg tgcgtaacag tgcacatacc ctggcgaaac tggcgacgcc
     6841 atttgccgaa ggtgaggcac tgtgtctttc cagcgtttcg catccggaat acattggacg
     6901 cgtcgcgaag ttctttagcg atatcggcgg gtgggcgtta ctaatgcatg gtactgaagg
     6961 cgaagtgtat gctaatccgc agcgctgccc gcagatcaat ctcattgacc gtgaagggat
     7021 gcgggtgctg tatgaaaaac aggaccctgc tggtagcgag ttactgccac aagcaaaaga
     7081 tccggaaacc acggcgcagt ggactgagcg ttgcctggct ggcagcgaag cgattcccga
     7141 atcgctgaaa atccagatgg cttgctgcct ggtggctacg ggtgaagcgg caactatcag
     7201 cgatggcctg gcgcgcgtta atcaggcatt ttaattttta tctcagccgg atgagccacg
     7261 tcatccggct tttaccccac cgcaatatga aattcctgca tctttattga ccttcccgcg
     7321 cccggcgtgc agcataaaaa aacaacaaac acataacata aacaggagtt aaccatggaa
     7381 agtggtcatc gctttgatgc tcagacgctg cacagtttta ttcaggctgt atttcgtcag
     7441 atgggtagcg aggaacaaga agcgaaatta gttgccgatc atttaatcgc ggcaaacctg
     7501 gcagggcatg attctcatgg tattggcatg atcccaagct atgtgcgttc ctggagtcag
     7561 gggcatctgc aaattaacca tcatgccaaa atcgttaaag aggcgggggc ggcagtcacg
     7621 ctcgatggcg atcgcgcatt cggtcaggtc gcggcacacg aagcgatggc gctggggatt
     7681 gagaaagcgc atcagcacgg tattgctgcc gtggcgctac ataactcgca tcatatcggc
     7741 cgtatcggtt actgggcgga gcagtgttca gcggcggggt ttgtctctat ccactttgtt
     7801 agcgtggtcg gtattccaat ggtcgcgccg ttccacggtc gcgacagccg ctttggcacc
     7861 aatcccttct gtgtggtttt ccctcgtaaa gataatttcc cgctgttgct tgattacgcc
     7921 accagcgcca ttgcatttgg caaaacccgc gtcgcctggc ataaaggcgt ccccgtgccg
     7981 ccaggttgcc tgattgacgt taacggcgtg ccgacgacca atccggcggt aatgcaggag
     8041 tcgccgttgg ggtcgctgtt gacctttgcc gaacataaag gctacgccct tgcagcgatg
     8101 tgtgaaattc ttggcggggc gctttctggc ggtaaaacga cgcatcagga aacgttgcaa
     8161 accagccccg atgccattct taactgcatg accactatca tcatcaaccc ggaactgttc
     8221 ggcgcgccgg attgtaacgc gcagaccgaa gcctttgccg agtgggtgaa agcctcgccg
     8281 catgatgacg ataagccgat tttgctaccg ggcgagtggg aagtgaacac gcgtcgcgaa
     8341 cggcaggagc aggggattcc attggatgcg ggaagctggc aggccatttg tgatgcggcg
     8401 cggcagattg gtatgtcgga agagacgttg caggctttct gtcagcagtt agccagctaa
     8461 aaaaaagccc gtccagtggc ggacgggcaa acaagggtaa cataggatca atgagggtta
     8521 gagcatatgc gtctgtcggc aaacagacag ggaaatactt gtgctggacg tagcgtaaac
     8581 gcctgatccg tccaacggtt cggattttgt aggcctgata agacgcggta gcgtcgcatc
     8641 aggcatctgt tgccggatgc ggcataaacg ccttatccgt cctacgaatt acttataaat
     8701 taccgcagta ccgcttaatt tattgttgtt ggtggcggaa gtgatgctgt atccactggc
     8761 accggctgcc gccgctttct cagccagttt cgcttccagg gcatcgaggg tggatgcgcc
     8821 atcggcagaa accacgccga ttttattcat gttctgtgcc tgggatgccg ttaccggttc
     8881 cgccgcgaac acgccaaatg acagagttga aagagccata gcagcaacaa cagtattgat
     8941 agttttcata attaatctct cgcaggtgat ttttgtataa gtgacgttgt ttcgtcgatg
     9001 tgatgagtat cacgtttttt tacgagagat aaaatcgaag agaattgacc gccttgttca
     9061 aataaattga ttgatatcta acatactgat aaaccattta tttgttgtac ttatggactt
     9121 gttttatgcc gcgtggcagt cacagcagcg ttaaaggcac ggaatgccac gcaaaataca
     9181 aattatactc gctatgcatc tcgccagttg tagttaacgt aactgtctgt ctttcgaacc
     9241 tctgcgatta tatcctgtat acgctggttt ttgtaaatct ttctcctgct gacaatgaat
     9301 acataagcgc acgccaggaa tggcttcccg acgggcttgc gggatggggg caccgcactc
     9361 ttcacattca tacaggcttt cgccacgcgg aatttcaccc cgagcgcggg caatcgcatc
     9421 ttcaattgta ctgttgatct gttcgttgac ggcgtcatcg ttagcccaag cggatgccat
     9481 atcgtcctcc ccatatcaat gcttgtacag ttaagtgtag ctaatatagg gacgaacctg
     9541 ggcagttcaa gcgtcagatc tccgaccatt cccgcagcag attatgataa agattgagca
     9601 gcgacaggat ctcttcattt tcaccatagc ggcttttcag cgactgaata tttttgtcca
     9661 gttcaaacag catggcgcgc tttttatcat cgcggatcat cgactggatc cacataaatg
     9721 atgccactcg tacgccgcgg gttacgggtg tcacgcaatg caggctgctg gagggataca
     9781 acaccagatc acctgccggg agttttaccc gatgttgtcc aaaggtgtca ttaacgacca
     9841 gttcgccgcc gtcgtagctt tgtggatcgc ttaaaaacag cgtggcagaa aggtcagtgc
     9901 gcatccagcc gttttgtgga tggctgcgta ctgcgccatc cacatggaaa ccataggttt
     9961 cattgttctg atagcgatta aacagcggcg tggaaagggt acgcggcaag gcagcggcaa
    10021 agaacaatgc atgttggtta accgcgttca gcacctcatt ttgcagtgcg gcgtataacg
    10081 cgctgcgggt gtctacttgc tgattgttct taacttgtgc accttgtgct ccggtggtga
    10141 cgcgtccatc cacccattcg gcttgttcca gttgttcgcg aaaatgggcg acgtcctgtg
    10201 gcgataacac gccgggaatg tggtacatca tacaagttct ccaaaaagtg gggcctgcgc
    10261 cccacatctg aatcagaaat gcatattggc tgtgagcaag aaggttcttg gctcgcccgg
    10321 gtgataacgg tagccgctct tgttgattga ggcgacgtaa tcggtatcaa acaggttgta
    10381 aacgtttagc tggaagtcga gattgcgatt aacccgatac cccagtttgg catcggcgac
    10441 ccagtaacct tctgtaaatg ctggcgttcc caccgcgccg tctgaacctt tatgtatact
    10501 gccgatatag cgtgcgcccg cgccaacaga gatatcatcg gttgcctgat attggctcca
    10561 taaggtgaag gcgtgctccg gggtatacgg cagcgatgag gaaccatcct gggcaacatc
    10621 tttgccgttt ttgatggttg ctttttgctg ggtatagccg ccaatcacct gccacgcggg
    10681 agtgatattc cctgccacgg atatctcata gccttcgacg cgtttcttac cgtattgcga
    10741 gtaagtcccg tcatcatttt gctcaacttc attttcgata tcagtacgga acagcgcggc
    10801 ggtgagcaac aggcgtttat ccagaacctg ccatttggtg ccaatctcgc tggtgttggc
    10861 tttttgcggt ttaaaatcgg tgcggttggc actgttacca ctgccagact gcgcaagggc
    10921 gaagttgttg ccgcccggag gctgctggga aacggcatag ttaatataga cattgccgtt
    10981 ttccgtcagg tggtacagcg ccccggcttt ccagttcacc agattgcccg acttggcggt
    11041 gtcgacggtg gtgactggag aaccttttgc cacaccagct gggcaggtga tggcaccgcg
    11101 tccgctgccg ccgcaggcgg tggcactgtc atattcagta tgataattat ccagacggat
    11161 cccgccgttc agctcaaaat cacgggtgat ttgcagcgta tcgaaggcgt aaattgcgaa
    11221 ggtatccgtc tgaccattgg cgtttgcgcc gttgcgcgtc aggccgccgg gatgaattct
    11281 gctgtcagga tgataaatat ttaccgccgg taacgtcacc ggattaacgc catagttagt
    11341 ctgcgtttca cgggtaaatt ccacgccggt actgacatca tgaccgatag aaccggtata
    11401 gaacgtcgag gtcaggttgg tctggttggt gagaatttta ttactcacat ctttggtatt
    11461 cgccgtgcgt gactaggtcc agctattcac atcgctggtg ggctgagtaa tattcgacgc
    11521 cccgcccata atcgccgtca tcaggtaatc ctgctttacg cgcgaccaac gggtagtatt
    11581 gcgaatggtg gtgttatcgt tgatgtcgtg ctcaaaacgc atggtggcgg tgtcggtggt
    11641 cgaatcgtcg taatcggaat ccgtgccgta aaagttatga gtatcaactt ttccggaatg
    11701 attcagggcc gccgctcccg cagatggggc agaatagccc ggcaaaccga tggtcggaat
    11761 gccgccgtct ggcgtgttgt gctgagtgac atgcagataa ttaagataca aacgatttgc
    11821 tgtaccaagg ccaaaagcga cagaaggggc gacgccgtaa cgctcatttt tgactttgtc
    11881 gcgtccggca tcgtgggttt tttcgcccat cacattcagg cgcaccgcgg tagtatcacc
    11941 aatgacctga ttgacgtcca gcgtgccgcg gcggaaccag gcgctgccaa tactggcgga
    12001 ggcgtcaatg ccggaatcat tgcgcggctg cttgctgatc atattgatcg agcctgtcgg
    12061 tgcgctgcgc ccgtagtcgg tgccggacgg ccctttaatc acttcgacct gctcggtatt
    12121 gaaggtgtcg cgcgagacgc tgccgatatc gcgaatacca tcaatataaa tactgttaga
    12181 ggtatcggca ccacgcatat aaatggcgtc gccagtggtg gagttaccgt tctcacccgc
    12241 aaaaaacgca cccacgc
//
LOCUS       DAZSNM010000118        12113 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZSNM010000118 DAZSNM010000000
VERSION     DAZSNM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.118"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..370
                     /locus_tag="ACHW42_002682"
     CDS             <1..370
                     /locus_tag="ACHW42_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944439.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="ACHW42_002683"
     CDS             complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="ACHW42_002683"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR8944440.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="ACHW42_002684"
     CDS             complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="ACHW42_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR8944441.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="ACHW42_002685"
     CDS             complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="ACHW42_002685"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR8944442.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="ACHW42_002686"
     CDS             complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="ACHW42_002686"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR8944443.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3448..5049)
                     /gene="tap"
                     /locus_tag="ACHW42_002687"
     CDS             complement(3448..5049)
                     /gene="tap"
                     /locus_tag="ACHW42_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR8944444.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5095..6756)
                     /gene="tar"
                     /locus_tag="ACHW42_002688"
     CDS             complement(5095..6756)
                     /gene="tar"
                     /locus_tag="ACHW42_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR8944445.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="ACHW42_002689"
     CDS             complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="ACHW42_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR8944446.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="ACHW42_002690"
     CDS             complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="ACHW42_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR8944447.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9394..10320)
                     /gene="motB"
                     /locus_tag="ACHW42_002691"
     CDS             complement(9394..10320)
                     /gene="motB"
                     /locus_tag="ACHW42_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR8944448.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10317..11204)
                     /gene="motA"
                     /locus_tag="ACHW42_002692"
     CDS             complement(10317..11204)
                     /gene="motA"
                     /locus_tag="ACHW42_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR8944449.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="ACHW42_002693"
     CDS             complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="ACHW42_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR8944450.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11912..>12113)
                     /locus_tag="ACHW42_002694"
     CDS             complement(11912..>12113)
                     /locus_tag="ACHW42_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR8944451.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2783 a         3388 c         2934 g         3008 t
ORIGIN      
        1 cactacgcta atgagttggg atgccgtgct ctatgatcta cgtagtgaaa aaattatcag
       61 taaagataat tacttgaccg acttaaaccg cagacatctt gaacttgttc ttcatactaa
      121 ccccaatcca agcggttcgg taacgaagat tttacgcact atttttacta agcaggtaaa
      181 gagtttaggg ccggaactct gtcatttctt gcaccatgaa ctgacgaaaa aacgttatga
      241 agtatggcat cactacgatg tactgcacta ccagtcatcg tcattttctc gttcacaatt
      301 taatgcattg ataaatttac tggcgcaaca atcacgcttc gctgatctaa aattatcaaa
      361 ctacaaataa ccatcctcca cgtactccct gaacgatacc cgccaccctg ctatcggctt
      421 ggcatcatcg cgcaggccga agttcaggca atacaaatca aaatccaaga ctatccaaca
      481 aatcgtccac ctgatcctga ctggctacca cgccggcttt gctggtatcg acctgaggtc
      541 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
      601 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaataa
      661 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
      721 taaagctggt atgcgcgggt acatctgcca gaaattgtcg tgtatctgtt accagttcac
      781 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
      841 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
      901 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
      961 tggcttccgc cgcttcggca atggcctgaa ccagccccag ttcccgcaaa ctgtcgcgca
     1021 gcatatgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     1081 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
     1141 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     1201 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     1261 aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc atccatattg
     1321 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     1381 tcgacaccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     1441 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     1501 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattgtt gccaacattt
     1561 gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     1621 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
     1681 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
     1741 ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc gaaggccgat
     1801 gacggttaac cgccgggccg tcgtgaattt tgatttgata atttgcgcca ctacgcgcca
     1861 gttccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaagaca cgttctccgt
     1921 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
     1981 tgaaaccggg cggcatatgt tgggtaatta acaatgccgg gctggaaagc ggcaacggtt
     2041 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
     2101 cagaactcaa caacggccct gccttcagcg ttgtcggtgc tgacaatggc ttatgtgctg
     2161 caaggctcgc ctttgctgcc gtacgcacct tttcagcaat catttcgcta tacgccagca
     2221 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc agctccagcg
     2281 cgcgcagcgt gacttctgat cctttgccgg tcaaggaaga aaccatcaca acgggcattg
     2341 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
     2401 ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcaacc agcggatcag
     2461 gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatctggc
     2521 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
     2581 tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc
     2641 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat
     2701 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
     2761 cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat caacatagtt
     2821 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
     2881 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga taccgcttct
     2941 ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc gtccgggcac
     3001 ggtgcccaat gtgtcagcca gagtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
     3061 cgccgcgctc catacgcgat cctcgccaga acggcgacgt gcgtgatccg cgagcagagg
     3121 gaaatgatga gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
     3181 ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac cgaaatccgt
     3241 cagtcccaga gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
     3301 agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
     3361 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
     3421 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
     3481 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
     3541 atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta ccagcgagga
     3601 gttctgctga gtcacctgat ccatctggct gacagcctgt gcaacctgct caatcccccg
     3661 ccgttgttct tccgacgccg aagcaatttc tcccataatg tcgttcacgc gggtcaccga
     3721 actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac cctgctggac
     3781 acgattgact gactcttcga tcagcccttt gatctctttt gctgcctgcg cgctacggct
     3841 ggcaagattg cgcacttcac ctgccactac cgcaaaacca cgcccctgct ctccggcgcg
     3901 agccgcttcc actgccgcat tcagggccag aatattggtc tggaaagcaa ttccgtcgat
     3961 aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca tggtgtgagt
     4021 catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt ttgccagttc
     4081 cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt gctccatact
     4141 ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga gatcgttatt
     4201 gcctgcgaca atctccgcga tcccaatgtg catctcatgg cttcccttac gcacatcact
     4261 caccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc
     4321 attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg
     4381 gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct
     4441 gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt
     4501 acgctggctt tgcgcactgg cggcttccag cacatggttg atctccagct gccaggcctc
     4561 aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag
     4621 ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact
     4681 tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca tcgccataaa
     4741 acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca tcaacgtttt
     4801 aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca gcgcggtact
     4861 ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc
     4921 gccgtgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc
     4981 aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg
     5041 attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaaaa ctcgtcaaaa
     5101 tgtttcccag tttggatctt gttcagtaat tcgcagtcgt ggctgtgccg gtggttgctc
     5161 actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacggaa
     5221 cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg cggcggcagc
     5281 tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg aaaccgccaa
     5341 tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat
     5401 gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg cgctttcgac
     5461 cagcaccgaa ccggtatcaa tgcgcgagac ggagtcttca atgagggctt tgatctcttt
     5521 tgccgccagg gcgctgcgac tggcaagatt acgcacttca cccgccacca cggcaaaacc
     5581 acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga ggatattagt
     5641 ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg aactatcggc
     5701 gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc
     5761 ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt
     5821 cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttcagt
     5881 acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc
     5941 tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct
     6001 ctgcgccagg tcacccattt cactgcgccc gtcaatggtc agggtattcg ccaggttacc
     6061 accggcgatt tcgcaaatgt gagcaataat ttttgccagc ggagtaagca acatacggcg
     6121 aatgccgtac cacgccacca gcagaatcaa taccaccacc agcgcgataa ccgccagttg
     6181 ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat acagtttttc
     6241 actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc cctgggttgg
     6301 ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt cagttaacgc
     6361 tgtgtaatag tttttatatt tttcatcaaa attacggctg gtagcgacca tttcaggtaa
     6421 cggtgccatg cttttgaatt ttttataatg cgtcgcggcc tgggccaatg ttttcctggc
     6481 gctatcgagc aattcaactt tggcgttact ttgttgattg gaggaatcca tcatcatccg
     6541 caccgctgaa cgactcaggt taatgcgcgt ttgcagcatt aaatcccagg ttgacgtcag
     6601 ctcgccctgc tgttcccgta attgattgga aaccacaaag ctcttctggc tatggtgaag
     6661 ggaagaaaaa aacaaactgc cggaaataag ctgtaacagt gcgaataccc ccagcaccat
     6721 taccaacagc gtgactacgc ggatacggtt aatcataagg caccttcctg aaaacaagtt
     6781 gatctcgtta tcggcaagaa ggggggaaac tttattgctg atgccacccg ccgcgaaatt
     6841 gaaataaaaa acccgatgcg cagatcatcg ggttcatttc aattgaggaa atcgggagaa
     6901 ttacgccact tctgacgccg cgctatctaa cagcgccatc tcttcgctgt tcagcagttt
     6961 ttcgatgttc accagaatca acatacggtc gcccagtgcg cccagtccag tgagatattc
     7021 tgttgaaagc gtcacggcaa actctggtgc cggacgaatt tgctccgccg tcaatgaaag
     7081 cacgtctgaa acgccgtcaa ccacgatgcc gaccacccgc tgtccgagat tcaggacgat
     7141 aactaccgtg ttgtcgttat agtccacatc cacctggctg aacttaattc gtaagtcaac
     7201 aatcggcaca ataacgccgc gcagattcgt gacgcctttg ataaacgctg gcgtgttcgc
     7261 aatccgtgtt acctgatcgt agccacggat ctcctgcact ttcaggatat caataccgta
     7321 ctcttcatca ccaagggtaa ataccagaaa ttcctggcct gacggctcgc tggccagctt
     7381 tgttacattc gtcataccgg tcatattgtt acctttttac tcattcaggc ggcggtgttc
     7441 gccatacgtt gttcgcggtt tatcgcctgc aaggcggaga catcaacaat cagtgccacg
     7501 ctgccgtcgc caagaatggt cgcagcagaa atgccgggga ctttgcgata gttactttca
     7561 aggtttttaa ccacaacctg gtgttgacca attaattgat ccaccagcaa ggcgtagcgg
     7621 cgaccgccac tttgtaagat caccacaatt ccctgggtgg cttcggtttt cgcgcccgcg
     7681 acgttgaaca ctttccacag ttcgacgatg ggcagatatt caccccgcac ttccagcacc
     7741 cgctcgccgc cggccagtgg atggagatcg gcttcacggg gttgcagtga ttccataaca
     7801 gcattcagcg gcagaatgaa aacttcatcc gcaacgcgta cggacatgcc gtcgaggatg
     7861 gccagcgtca gcggcagtaa aatgcggatc gtagtgccag taccctgctt cgactggatt
     7921 tcgacatgac cgcccatctc ctggatatta cgtttaacga cgtccatgcc gacgccgcgc
     7981 ccggagacgt cggtgacctg ctctgccgtg gagaagcctg gtgcaaatat cagcattgca
     8041 acttcgtcgt cgctcatgtt ttcgctgaca gtcaaacctt gcgaggccgc ttttgccaga
     8101 attcgctcac ggtttagccc cgccccatcg tcggtcactt caatgcaaat gttgccgccc
     8161 tgatgttcgg cagacagaat taaatttccg acgctgtttt tacctgcggc gagccgtttt
     8221 tctggcagtt caataccgtg atcgaggcta ttgcgtacca ggtgggtcag cgggtcgata
     8281 atgcgttcta tcaggctctt gtcgagctcg gtggaactgc ccaccagcgt cagttctacc
     8341 tgcttgccga gttttcccgc cagatcacgc accagccggg gatagcgact aaagacatat
     8401 tccatcggca tcatgcgaat cgacatcact gattcctgca aatcacgggc gttacgttgt
     8461 aactgcccca tgctggtaat caaatcacca tgattaaccg ggtccagttc gctggaacgc
     8521 tgggcaagca tggactgggt gataaccagc tcgccgacga ggttaattaa ttgatcaacc
     8581 ttttctaccg ctacacggat gctggtggat tcactgctgc gcgtcgtttt ttcccgctcc
     8641 acgcggccgg ttggcgcttg ttcggctgcc agtttaagca caggtggggt agctattttt
     8701 ggcgaaactt ctactgtttc aaaggtaatc tgatcggctt caatcacaaa acagagtacc
     8761 gctgtgatgt catcttcggc gatatcgccc ggtaatattg ccgagagaga atccgcccct
     8821 ttcaccacgt catttaacgt tgtcagatgc cccagctctt cttccagcag gtcgacttcg
     8881 ccggccttca ggcgcgaaag gataattcgt cgcggcaact gactgcgact ttgctcatct
     8941 tgcggttcac ttttggcaac cacacttaat cgggtcactg cggatggcgt ttcgcctttc
     9001 gcttctaatg ccagttgacg caaggcctgg cagatataat cgtagctggc ggcatccggc
     9061 tcttgcgact gtttataagc gtcgagctgt tcttgcatga tgtccttcgt ttccaaaaac
     9121 agattgataa tgtcggtgtt gagttgcatc tcacctcgtc tggcttcatc gagcaggttt
     9181 tccatcagat gcgtggtttc ctgcaaaacg ctgaagccaa aagttcctgc ccctcctttg
     9241 atcgagtggg cagcccgaaa gatggcattc aattgttcgg catctggcgc ttccggctgc
     9301 aaaaccagca gatgctgctc catatcagcc aacagttcgt ccgcttcatc aaaaaatgtc
     9361 tgataaaaat cgcttatatc gatgctcacg ctgtcacctc ggttcggctg atggcattgt
     9421 gggaacactg acctgtggtg caacctcagg tttttctagg gcgcttactg gctcattctg
     9481 gctttcggcg ttttcatgca aaatggcctg ttcggcttgt ttgttcagta ccaacaggct
     9541 gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt aagcgcatcg ttgccgccat
     9601 gccgacgaca cgtaacactt tgccgctatc caaccctccg accatcagtt cgcggcggga
     9661 tgcattggcc cgatcggcag aaagctccca gttgctgtaa cctttctcac cgctggcgta
     9721 gggaaaatca tcggtatgac ctgaaaggct aatacggttg ggaataccgt tcagcacagg
     9781 cgcgatggcg cgcagaatgt cgcgcatata gggttcgaca tcggcactgc cggttctaaa
     9841 catcgggcga ttctggctat cgatgatctg aatacgtaga ccttcctgga ccagatcgat
     9901 tttgagatgg agacgtaacg cccgcagttt cggatcggac tctatcaact ggtcgagatc
     9961 accccgcaat ttccgcaatc gactttgctc catgcgtttt ttcagctctt cgatgttcgg
    10021 ctgcttattc acttccccct ggctttgggt gtaatcatca ccaccgccgg gaattgggct
    10081 ttcactattg gaaatgcgat cgccgcccgt aaccgctgtc gccagtggag tccggaagta
    10141 ctccgcaatc tgaatcaact cttttgggct ggagatggag atcagccaca tcaccagaaa
    10201 aaaggccatc atcgcagtca taaagtcggc ataagcaatc ttccacgatc catgtgctgc
    10261 cccgtggctt ttggctttgc gtcgtttgac gacaataatc ggatgcgctt gattcttcat
    10321 gcttcctcgg ttgtcgtctg ttgttgcgga tttttcaccg cacgcacatg ctcttccagt
    10381 tcaataaacg acggacgttc gctggaatag agcgttttgc gaccaaactc aacggcgata
    10441 ggcggtgcgt aaccgttcag attagaaagc agagtgactt tgacgcactg catcattttg
    10501 ctggtttcgg cgcttttctg acgtaaaaca ctcgctagtg gggaaataaa tccgtaagcc
    10561 aataaaatac cgaggaaagt ccccaccatc gcatgtgcga taagcgcccc cagctcggcg
    10621 gcaggacgat cggctgaacc taacgcgtga acgcccccca ttacagccgc gacaatacca
    10681 aacgccggaa gtgagtcccc gaccagcgcc agactgttcg ccgggacttc tgcctcgctt
    10741 tcgtgcgtct caatctcttc atccatcagg gcttcgattt cgaaggtgtt catgtgaccg
    10801 ctgataatca ggcgcagata atcgacgata aaatcaagca tgacgctatc cgcgaggatg
    10861 cgtgggtagc tggcgaagat ctcgctctca cggggatttt caatatcacg ttccagcgaa
    10921 aacatcccca tctgccgcga tttcgccatc aaccgataaa gcagagccag cagatccata
    10981 tacattgctt tggtgtattt ggagcgacga aacagcaacg gcagcgcttt cagcgtgcct
    11041 ttaatcgctt tgccattatt gccgacgata aacgacccaa tccctgcacc ggcaataatc
    11101 accagttcag cgggttgata gagtgctcca aggcttccac cggtcatcaa ataaccgccg
    11161 aaaactgtac cgagaacaac caggtaacct aataagataa gcacgacatc atccttccac
    11221 tgttgaccat gacaggatgt tcagtcgtca ggcgttaacg cgcgattggg gaaaaaaaaa
    11281 gcagcggtac gtcgttaccg ctgctggaat gttgcgccac accgtatcag ttaaacagcc
    11341 tgtactctct gttcatccag cagttgtggg ataatatcgg caggattctg ggaaagttta
    11401 cgtcttttta ctgcccggga tggcggttga cataagctgc aggcaaagct gccaacaggc
    11461 tggtgagcgt gggtaataaa attgccgccg cagcagttgc agctggaaag ttgcagtaat
    11521 ccactttcaa caaaccgcac caatgtccag gcacgggtta atgccagcag tggtccttct
    11581 tctgcttgtg ggcactgttc aaggtataaa cggtaggctt tgatcaccgc atcgacgcca
    11641 ttacacaaac cggttttcag taaaaactgc catgcattac agaacatcga agcatgaacg
    11701 ttttgttccc aggtcataaa ccagtcggtt gagaatggca gcatgccttt cggcggtggg
    11761 cttccgcgca gttctttata aagttttatc aggcgtccgc gacttaactg tgtttcgctt
    11821 tccagcatct gcaaacgagc gcccagggtg atcaattcca ttgccagctg aatatcccgc
    11881 gcttcctgaa caatgctttt ttcactcatg atcaggccct tttcttgcgc agcgcttctt
    11941 caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta tgaatttgct
    12001 ggagatcgtc aacgcgggaa tcttgcgtca actgagtaat cgtctggtgg ctgtcaaaac
    12061 ggaagtgaca aaccagctga ttggtttctg ccagcttaac catttgcgga aga
//
LOCUS       DAZSNM010000119        12049 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZSNM010000119 DAZSNM010000000
VERSION     DAZSNM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12049
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.119"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(4..507)
                     /locus_tag="ACHW42_002695"
     CDS             complement(4..507)
                     /locus_tag="ACHW42_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR8944452.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            complement(552..1515)
                     /locus_tag="ACHW42_002696"
                     /pseudo
     CDS             complement(552..1515)
                     /locus_tag="ACHW42_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(1829..2965)
                     /gene="hemW"
                     /locus_tag="ACHW42_002697"
     CDS             complement(1829..2965)
                     /gene="hemW"
                     /locus_tag="ACHW42_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR8944453.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(2958..3551)
                     /locus_tag="ACHW42_002698"
     CDS             complement(2958..3551)
                     /locus_tag="ACHW42_002698"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR8944454.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(3559..3849)
                     /gene="yggU"
                     /locus_tag="ACHW42_002699"
     CDS             complement(3559..3849)
                     /gene="yggU"
                     /locus_tag="ACHW42_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR8944455.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(3846..4412)
                     /gene="yggT"
                     /locus_tag="ACHW42_002700"
     CDS             complement(3846..4412)
                     /gene="yggT"
                     /locus_tag="ACHW42_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR8944456.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(4430..5134)
                     /gene="yggS"
                     /locus_tag="ACHW42_002701"
     CDS             complement(4430..5134)
                     /gene="yggS"
                     /locus_tag="ACHW42_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR8944457.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            5152..6132
                     /locus_tag="ACHW42_002702"
     CDS             5152..6132
                     /locus_tag="ACHW42_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR8944458.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            complement(6308..6724)
                     /gene="ruvX"
                     /locus_tag="ACHW42_002703"
     CDS             complement(6308..6724)
                     /gene="ruvX"
                     /locus_tag="ACHW42_002703"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR8944459.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(6724..7287)
                     /locus_tag="ACHW42_002704"
     CDS             complement(6724..7287)
                     /locus_tag="ACHW42_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR8944460.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(7396..8346)
                     /gene="gshB"
                     /locus_tag="ACHW42_002705"
     CDS             complement(7396..8346)
                     /gene="gshB"
                     /locus_tag="ACHW42_002705"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR8944461.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(8359..9090)
                     /gene="rsmE"
                     /locus_tag="ACHW42_002706"
     CDS             complement(8359..9090)
                     /gene="rsmE"
                     /locus_tag="ACHW42_002706"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR8944462.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(9170..9877)
                     /gene="endA"
                     /locus_tag="ACHW42_002707"
     CDS             complement(9170..9877)
                     /gene="endA"
                     /locus_tag="ACHW42_002707"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR8944463.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(9972..10469)
                     /locus_tag="ACHW42_002708"
     CDS             complement(9972..10469)
                     /locus_tag="ACHW42_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR8944464.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            complement(10546..11940)
                     /gene="galP"
                     /locus_tag="ACHW42_002709"
     CDS             complement(10546..11940)
                     /gene="galP"
                     /locus_tag="ACHW42_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR8944465.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         2954 a         3089 c         3062 g         2944 t
ORIGIN      
        1 ccattaactg gcctcctgcg caggttgttt cagttgttga atgctttgat taataaaaca
       61 caaggcataa aacatcatta ccactccact taatggcaga atgatatata cgtatcccat
      121 cggtatttgc atagcggcag atacctgtga tgttgccagt gttttatcaa ttaatttcag
      181 gccaccagtc acaatgaccg aaccggcaaa acctaaaatg agaacgttaa tgactatact
      241 aagaagtaat tgtttgcgct tattcagggc gagagcaaat aaatcaatag agagatgtcg
      301 ctgcgcgccg acggtatatg cagcacctaa taatcctacc cagatcatca aaaagcgtgc
      361 cagctcatcg gttaatgtac ttggctggtt aagtacgtag cggctaaata cttgccagac
      421 tacgcataca accagagcaa gcattacggc gacagaaaac gtggcaatga cgcggtctac
      481 cgcaagtttc aggcggttaa atatcattgt aagcatcctg gaaaatcagg gccagaggct
      541 ggcccggaat attattgagc agcgttgtcg aatttctcta acaaagcagc ttgttttgga
      601 tcttttttga agtcgtcaaa cagaggttgc acggctttac ggaatggcgc tttatccact
      661 tttacaattt caccccccat agctttcgcc tgagcacgtg tgtcagcgtc aattttttcc
      721 cacaattttt gttgataagc ttcggatttc ttcgccgctg tttcgaggat ttgttgctgt
      781 tctggggtta atttattcca tgttttggtg gagataacca aaaagtcagg aattgaggcg
      841 tgttcatctt cagaaaaaac tttggcaatt tcaatatgtc gagtctgcat ccaggaaggc
      901 acgttatttt ctgcgccatc tactacacct tgctgcatcg cagtataaac ttcgccaaat
      961 gaaattggag tgggtgaacc acccatcagt tcaatcattt tgatggtggt cggacttggc
     1021 tgaacacgaa ttttcagtcc tttaagatct tctggctttg taatcggttt tttcgcatag
     1081 aaactgcgag tacccgctac ataagctgat aaagcaaaaa agcctttgtc ttttgttgag
     1141 tccataattt ctttaccaac ttcaccaaaa acaacttaat aagaacggca aattatagat
     1201 agcataaata ttatcaaaag actccagatc actggcggaa cctttggtca tatccagtgc
     1261 gccattttgc aacaattcca tagtttctcg cgcattaccc atctggctgc tgggataaat
     1321 acggatcacc atattacctt ttgacagctg ttttacctct ttcgccagct cttcaaaact
     1381 ctgatgtaca acatgacttc tttccaggtt gtgtgctaac tttaacgcca ctttttctgc
     1441 ggcgtttacg ccagtgctaa ccagagccag caaggatgcg cagactaatg agcgggtaaa
     1501 tgacctcatt ttcataaaaa aactccctgt tgaaggatgg aaattggttg atatctaatt
     1561 gtgcttatca gtgatttgtg cgacaaattg gttaacctgt ctgacaactt gtctatgttt
     1621 atcacaactt gaaataaagt cagaaattaa atattaaatt tgtgcgccag gttgtacata
     1681 tttataatca atgaatgatt agtaaatatt cactaaatat atataataaa tgtcgtcgat
     1741 atttattcag taagaaatat atttctgccg aggttaatta acggggggat aaaagaagtg
     1801 attaatgcgc ccgcaggcgc attaatgatt actcagcgag gaataattca agaagtgagt
     1861 ttaagaataa tttaccatgt tccgttatct gccagtaatc cgcacattcg gtgagataac
     1921 cctgggcaat cgcctcgtct aactgtgggc gaatcacatc ttcgcaaagc ccggtatacg
     1981 caataaactc cacgcgcggc gcggcctcca gcagacggaa gcgattcata aagaactcaa
     2041 acggcttatc tgcggcttcg acatcacgct ggctttccag ataccttcct tgcataaaac
     2101 cacgaggatg acgcgtttta gtggtacgca gaatgcgccc atccgggaag gtcactttgc
     2161 cgtgcgcgcc gcagccaata ccgatgtagt caccaaagcg ccagtagttg agattgtgct
     2221 ggcactgata accgggtttg gcgtaagcgg aagtttcata ttgctgataa cccgctgcgg
     2281 ttaataactg atgcccctgt tcgaaaatat cccacagcgc gtcatcgtcc ggcagcaccg
     2341 gcggtcgcga gccaaacagc gtattaggtt cgatagtcag ttgataccag gaaagatgcg
     2401 gcggattcag ttcaatggcc tggcgtagat cgccaagcgc ctcttccagt gattgatccg
     2461 gtagtccatg catcaaatca aggttaaagc tacgtaaccc taaaccgctc gccagctttg
     2521 ccgctcgttt cgcttcttgc gggccatgaa tacgcccaag tcgtttcagc ttttcttcgc
     2581 taaaactctg cacaccaata gagatgcggt ttacaccagc acgctgataa tcgacaaagc
     2641 gatccgcttc taccgtgcct gggttcgctt ccatagtaat ttctgcatcc gctgccagcg
     2701 gcaaacgcgc acgcacgccg tctagcagcg tttgcatcgc cgggccggaa agcaggctcg
     2761 gcgtaccacc gccaataaag attgtcttta cttcacggcc ctgagcgtaa gccacatcat
     2821 tgtccagatc gcaaagcaga tgctgaacat aatcgtcgtg cggcacttct cctttcaacg
     2881 cgtgagagtt gaaatcgcag tacgggcatt tctgcacgca ccacgggatg tgaatgtaga
     2941 gactcagcgg cggtaattta accattacgt aaagcgtcca gcagcagttt caacgcctga
     3001 ccacggtggg aaatggcgct cttttcttcg cgggtcagtt cggcggcggt tttcccttcg
     3061 gaaggtacga agaagattgg atcataacca aagccaccag tgcccgctgg ttcgcgagta
     3121 atcacgcccg gccagctgcc gtggcatacc agcggagtcg gatcttccgc gtgacgcaga
     3181 tataccagca cgcagtggaa acgcgcctga cgttggtcgt ccggtacgtc tttcagtgtt
     3241 tccaacagtt tttgcagatt cttttgatcg gtcgcgtctt caccggaata acgcgcggag
     3301 taaatacccg gcgcgccacc cagcgcatct accgccagac cagagtcgtc ggcaattgcc
     3361 ggtaaaccgg tcacttttgc cgcatggcgc gctttcagaa tcgcgttttc gataaaggtt
     3421 aggccggttt cttcagcgga atcaacgccg aggtctgttt gggccacgat atcaagacca
     3481 aagtcgctaa gcagcgacgc cagctcacgc actttaccgg cattgccggt tgcgaggaca
     3541 actttttgca taggatacct aattaattaa cgccgcgatt tctggcggga tttgttgcgg
     3601 attaatgatt ttaatttgtt tgtggcggcc aagttcgcct ttttcaatca ccacctggct
     3661 tttggcaacc cggaattgct taccgagaaa cttcaccaga tgactgttgg cctggccgtc
     3721 aaccggcggc gcggtaatgg cgactttaac ttcgtcgcca tgtaaaccga caatagaatc
     3781 acggctggct ttcggctgaa tatagagccg taaaaccaga ccgtcatcat taactgttac
     3841 ggcactcata acgccatcca cagccccggc agcagcatgt ttccggttgc ctgtaatact
     3901 tctgcgacac ccatattgat ggcatacagc agcagaacga ggatcatcgg cgagaaatca
     3961 atcccaccca ttgccggtag caagcggcga atcgggcgca gcagcggatc ggccagctga
     4021 atcagcacgt attcaatcgg gctacgaccc tggcttaccc agctcataat cgccatcacc
     4081 agcaggaccc agaaaatcag caagccgatg gttttcagca gaatcagtaa accggcaatc
     4141 cagatgattg gcaggaaggt caccacttca aacagcacaa tggctttgat aaaactgaga
     4201 atataggcaa ccagcagcga ggcgctgtca atgggaccca ttgccggaat aacgcggcgc
     4261 agtggcccga taattggctg cgctaccttc actacaaact gtgagaaggg ggtgtaaaaa
     4321 tcacaatgag cacactgcat ccagatgcgt aataacagca ccattgtata cagctcaatg
     4381 accgttgaaa gcaggaaagt caacgtattc atggcgttcc tttaattcct tattttttag
     4441 agtaatcacg cgcaccaaaa attgcagtgc cgatacgaac catcgtgcta cctgccgcaa
     4501 tggcggcttc catatcgtcc gacattccca gagagagcgt gtcgatatgc gggtagcgcg
     4561 ttttcagtcc ggcaaatgct acagccattt ggcgtgcaac ttcaaactgc cttacatatt
     4621 ctgactcaag cgcagggatt gccatcagcc cgcgcagacg taaacgcggt agttcagcga
     4681 ccgccgctgc cagctcgtcc agttctgcca gttgaatccc ggacttactg ttttcatcac
     4741 taatattaat ttgaatcagt acgttaagag ggggaagttc tgccgggcgc tgctcgttga
     4801 ggcgggtagc aatgcgcaaa cggtcgatgg tatgacacca gtcgaaatgc tctgccacta
     4861 ggcggctttt attagactgc aacgggccaa taaaatgcca ttctaatcct gttacgccca
     4921 gttcctgaaa gtggcgaatt ttatctaccc cttcctgaac gtagttttca ccaaattgac
     4981 gctgcccggc atcaatggct tctgcgatgg cgctcgcagg ttttgtttta ctgactgcaa
     5041 gcagcgtaat ttcttctgga gaacggccgc aacgcgttgc agccgctgag attttgtccc
     5101 ggacctgtgc caggttatgc gcaatatcgt tcattttccg aggatcttag tatgaatatg
     5161 gaagaaattg tggcccttag tgtaaagcat aacgtctcgg atctacacct gtgcagcgcc
     5221 tggcccgcac gatggcgcat tcgtggcaga atggaagctg cgccgtttga tgcgccggac
     5281 gtcgaagagc tactgcggga gtggctggat gacgatcagc gggcaatatt gctggaaaat
     5341 ggccagctgg attttgccgt gtcgctggcg gaaaaccagc ggttgcgtgg cagtgcgttc
     5401 gcgcaacggc aaggtatttc tctggcatta cggttgttac cttcgcactg tccacagctc
     5461 gaacagcttg gtgcaccacc ggtattgccg gaattactca agagcgagaa tggcctgatt
     5521 ctggtgacgg gggctacggg gagtggcaaa tctaccacgc tggcggcgat ggttggctat
     5581 cttaatcaac atgccgatgc gcatattctg acgctggaag atcctgtgga atatctctat
     5641 gccagccagc gatgtttgat ccagcagcgg gaaattggtt tgcactgtat gacgttcgca
     5701 tcggggttgc gggctgcatt gcgggaagat cctgatgtga ttttgctcgg agagctacgt
     5761 gacagtgaga caatccgtct ggcgctgacg gcggcagaaa ccgggcatct ggtgctggca
     5821 accttacata cacgtggtgc cgcgcaggca gttgagcgac tggtggattc atttccggcg
     5881 caggaaaaag atcccgtacg taatcaactt gcgggtagtt tacgggcagt gctgtcacaa
     5941 aagctggaag tggataaaca ggaaggacgc gtggcgctgt ttgaattact gattaacact
     6001 cccgcggtgg ggaatttgat tcgcgaaggg aaaacccacc agttaccgca tgttattcaa
     6061 accgggcaga aggtggggat gttaacgttt cagcagagtt atcagcagcg ggtgggggaa
     6121 gggcgtttgt gacgttgcca tgcccgatgc gcaagtttat agggcctata aaggcaataa
     6181 atgtctgatg cgacgctaac gcgtcttatc agacctacag ccgctgccag atgtgtgctg
     6241 gataaggcgt tcacgccgca tccggcaaga tggcggtaac gactatccgc caggctttta
     6301 gatcgcctta atatccctgc tcgaaatagc tttcgagaat aataaccgca gaggcagagt
     6361 caactttgcc tttgttgagc gcccgatagc cgccctgttc aaaaagaccg gaacgggctt
     6421 ccacagtgct aagacgctcg tcatggagct ttacttcaac accgaaacga ccatgaatac
     6481 ggttggcaaa tttgcgcgct ctggcggtta acggctgttc ggtgccgtcc atattcagcg
     6541 gcaaaccgac gatgatttcg tcaggctgcc actctttcag taaacgttcg atgaggttcc
     6601 agtccggcgt accgtcctgc gctttaattg caggcaaagg gcgagcggtg ccggtaatgc
     6661 gttggccgac cgctacgcca atgcttttgg tgccgaagtc gaaggcgagt aaggttccac
     6721 tcatcaggcg tgtcctgcca caccaggcat ggtgagaata tccacaccaa tcagttttgc
     6781 cgcctcgcgc cagcggtcgg caatcggcgt tttgaacaga atattcagat ccgccggggc
     6841 cgttagccac gcgttatcga gaatttcttg ttccagttga cctttttccc aggaggcata
     6901 gcccagagcc accaatacgt cagacggttg tttatcggtg ccgagcgttt ccagcacatc
     6961 gcgggaagtg gtcatcaccg tgttgtctga aatgcgaatg ctggaagcaa aattggaggg
     7021 cggagtatgc aaaataaacc cgcgatcttc agccagtggg ccgccgagca taaccggttt
     7081 atccagacgg attgattcat cacgcggctc cggcgtgatc ttcagctttt ccagaatccc
     7141 ttcaattttg agattttcca gcggcttgtt gacgatgatc cccattgcac cattggtatt
     7201 atgttcgcaa atgtagacca cggaacgacg gaaaatcgga tcctggagag caggcatggc
     7261 aataagaaag tgatgctgta aattcattgt cagaggttct gtttcctggt tcaaaaaagc
     7321 aacagcgccc agtatgcgtg gaaagaaggg cgctgtcact cagagtctca acgagatcct
     7381 tctcgctaag gtgggttact gctgctgtaa acgtgcttcg atggcatcca ttaacattcc
     7441 ggtgatcgac accggaaact ctgcttcaat ctcacgaata caggttgggc tggtgacgtt
     7501 aatttcagtc agacggtcgc cgatgatatc cagaccaaca aaaatcagcc ctttttcttt
     7561 cagcgtcggc ccgatctgac gggcgatttt ccagtcactt tccgtcagcg gacgaggttc
     7621 accgcgacca ccggcagcca gattgccacg ggtttcgccc ccctgcggaa tacgcgccag
     7681 gcagtacggt accggctcgc catccaccac cagcacgcgt ttgtcgccat ctttaatggc
     7741 tggcaggtaa ttttgcgcca tgcagtagca agtgccatgc tcagtcaggg tttcggcaat
     7801 cacgccgagg tttggatcgc cttctttcac gcggaaaatc gacgcgccgc ccataccgtc
     7861 cagcggctta agaatgatgt cgctgtgttt ctcccagaac gcttttagct gcgctttatt
     7921 gcgcgtaacc agcgtttctg gcgttaagtc agagaaccag gcggtaaaca gtttctcgtt
     7981 acagtcgcgc aggctctgcg gcttgttaac gatcagcgtc cctttctctt cggcacgttc
     8041 cagaatatag gtcgcgtaga taaactcggt atcaaacggc gggtctttac gcatcaggat
     8101 cacatcgaga tcggccagcg gcagatcctg ttcaccgacg aacgaaaacc actcttcgta
     8161 gttctgcttc acgttcagcg tgcgggtatg ggcgcgggct tcgccattga tcagatacag
     8221 atcgcccatc tccatatagt gaagttcgta accacgacgc tgtgcttcca gcaacatagc
     8281 gaaactggaa tctttcttga tgttgatgtt tgcgatgggg tccatcacga tgccgagctt
     8341 gatcattatt cttctccgtt agcccaaatc gccaaatcgt acttgtagcg cggtaatggc
     8401 ggtgagcgca gttgtctctg tacgcaaaac gcgaggtccc aacaggatat cagtaaattg
     8461 atagcgggca gtcatggcaa tttcatctgc cgataaaccg ccttccgggc caatcagcag
     8521 gcggacgcgt tcaaccggta aaggcaacgt attgatgctg ttactggcgc gcgggtgaag
     8581 attcagtttc agtccttcat cctgctctgc acaccaggct tccagatcca tcgctggacg
     8641 gatttccggc acccggttac gaccacactg ctcacaggca gcaattgcaa tcttctgcca
     8701 ctgctgaagc ttcttgttca gacgttcact atccagttta acgccgcagc gctcagaaaa
     8761 aagtggcgta atgaggctta caccgagttc gatcgatttc tggatagtaa attccatttt
     8821 ttcaccacgc gacatcacct gaccgaggtg aatatgcagc ggagattcgc gatcgtcgat
     8881 ctggccttcc agcaccttca cttccacgct ttttttgctg gcgctggtaa tttcggcgtc
     8941 aaagacctgg ttgctaccgt caaacaattg caacgcctgc cccggcccca tgcgcagtac
     9001 gcgcccgata tggttggcgg catcttcgca aagcgcgatg tgagaatggc tggtcagtgg
     9061 ttcaggatga taaatgcggg ggatacgcat agttaaaaat ccgcgtcgtc tctccacgcg
     9121 gttgtacgcg tggggtaggg gttaacaaaa agaatcccgc tagtgtaggt tagctctttc
     9181 gcgcctggca agcgcgttgc acatacgggt tatgattgcc ctgcaccttc gcgatgcgtt
     9241 catcgcgctc gcactcccag tcggtaaccg gatacatctt gttccatgcg ttgaacagct
     9301 gcgtttgctg gcgagagagt gtcaggttgt attggtcgcg catatagaag taggtgcgcg
     9361 caatggcacc gcgtgcacgc gctggtggtt cggcagcttt ttctttgaaa tcgaccttca
     9421 tggcgcattg accgtactgg ccttcgccgc cattccactg gctgtacata aaattgccgc
     9481 gatcgccatt cacctcgccg actgacggct gcaggttatg catatcgctt tccatcttgc
     9541 gatagaccgg atctttagcg cagtttttac gtccaccgtc ctgccagcac tggcgctggt
     9601 gaccgaattg ccaggcggga acgacgtgtt cccactctac gcggctggcg cggttttcat
     9661 ttttgcgcac ctgatagccg cacgattgca gatcaacaac gccttttttg ccctgccagt
     9721 taattttaca tccgcaataa aacgtaccgg gcgcatcagc gtggactttt accgccgcgg
     9781 ctttcgcctg agaaaaacta ttgatacctt cggccaacgt cgggccggaa aatgctgcgc
     9841 tcagtaccac cgcagcaata gacaaataac ggtacatcac ttactccgtg ttaaaacgag
     9901 ccagcaacgt agcgaaagtt gttttggtat gcaatcagcc agatcaggaa agttcctgat
     9961 agttcaggtg gttatttcgc aaccagctgt tcaccgcagt gaacacagcg atagatggcc
    10021 tcgccacgaa ctacgcgatt atggcggcgt acggtaagct gatgttcctg gcacttacag
    10081 cggtagggga aggtgttgcg acgcacggat tgcagttcga actgatgcgt acgacgggcg
    10141 ggaacgccca gcacgctttc catcatccac ttccactctt tgccatgtgg cgctacgcgg
    10201 ccgaaatgtt tccataccag caaatgtgcc agttcgtgcg gtaccacttc ttcaataaaa
    10261 gcttcactgt tttccaacag caaaacggga ttgaggcgga tttcatagct ttccagccag
    10321 gctgttccgg cggaggttcc gcgctgggtg taagagagtt ttggctccgg gtagttacgc
    10381 cctagcttca ggttggcctg ggcgagtttt tcccgcaggc ggcgcataac ggcctgttgg
    10441 atggcgatag ggagacggga tgttttcata gcggcagagg atagagcgaa gaaaagatga
    10501 ctgcaagagg cggcttcctc cgcgacggga ggaagcctga ggagattaat cgtgagcgcc
    10561 gatttcgcgc agtttacgac ctttcatcag attacgttca atatgttcca gcgaaacgtg
    10621 tttggtttcc ggtaccagcc acaatgtcag caggataaac agtacgttca gagccgcata
    10681 cacccagaag gtgttggcgt tacccagcgt gttgagcatg gtcaggaacg ttgcgccaac
    10741 gatcatgttg gcaatccagt tggtggcggt ggagcaggtg atgccaaaat cgcggccttt
    10801 cagcggctga atttcggagc acagtaccca aatcagcgga ccggcactca tggcaaaacc
    10861 gacaataaac atcagcagca tggcgatggc gaaatactgc gccgacggag agtggatacc
    10921 gatatgcatc attgtaccga gtacgcccat gccagcagcc atcaccagga agcccagcgt
    10981 tagcgttggt ttacgtcccc agcggtcaac aaggccgatt gcgataaagg tggcaagtac
    11041 gttggtcagg ccgacaatca ctgtccccca catttgctcg gtggtgttgg tataacccgc
    11101 cagttcgaag attttcggcg cgtaatacat gatgacgttc atcccggtga attgctgcat
    11161 tacctgtaac agtacgccaa ggaacaccgc gcggcggaag ttgctattct ctttaaacag
    11221 cgcccagcca ctctgtttaa cctgcaaact ttcacggatt tcatccagtt cgcgtttcgc
    11281 ttccgcgctg gtgtcacgca ggcgtagcag cacgcgttcg gcatcaacaa aacggcgttt
    11341 ggcggcaaac caacgtgggc tgtctggcag gaagaagaca ccaatcagca gcaaaattgc
    11401 cgggatgata atcacaccca gcatccagcg ccatgcaccg gtgtagctga aggcggtatc
    11461 agaaagataa gcaccgagga tcccgatagt gatcatcaac tgatacatcg agatcatact
    11521 gccgcgaatt ttttccggcg caatttctga caggtacagt ggtgcggtat aagaggccac
    11581 acccaccgcc agccccagca gaacgcgcga aagaatcagt acttcaacgt ttggcgcagc
    11641 cgcagagaac agtgaaccgg caacaaacag gattgcgccg atcatcaggc tctttttgcg
    11701 cccgagttta aaggagagcc agccgatgcc caccgcaccg actgccgcac cgaacatcat
    11761 ggagcttacg acccattctt gcgtgtgcga agttatctgg aattcatctg caataaacgg
    11821 cagtgcgcca gcaattacac cgatatccag gccaaagagt aatcccgcca gagcggcaag
    11881 gaagcagacg aaaaacgtca ttgccttgtt tgaccgcccc tgttttttag cgtcaggcat
    11941 gatgccctcc aatatagtta ttttttattg tgaattaaga taggtgagta cgacgtaaaa
    12001 agatgtgaag caaatcacat cagtgtaatc gcttacacta acatttatc
//
LOCUS       DAZSNM010000120        12029 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZSNM010000120 DAZSNM010000000
VERSION     DAZSNM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12029
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.120"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            81..350
                     /locus_tag="ACHW42_002710"
     CDS             81..350
                     /locus_tag="ACHW42_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFR8944466.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="ACHW42_002711"
     CDS             344..709
                     /locus_tag="ACHW42_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR8944467.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACHW42_002712"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACHW42_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR8944468.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="ACHW42_002713"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="ACHW42_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR8944469.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="ACHW42_002714"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="ACHW42_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR8944470.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="ACHW42_002715"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="ACHW42_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR8944471.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACHW42_002716"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACHW42_002716"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR8944472.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACHW42_002717"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACHW42_002717"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR8944473.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12029
                     /gene="malT"
                     /locus_tag="ACHW42_002718"
                     /pseudo
     CDS             9674..12029
                     /gene="malT"
                     /locus_tag="ACHW42_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
BASE COUNT         2822 a         3274 c         3024 g         2909 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgcca atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaata
//
LOCUS       DAZSNM010000121        11986 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZSNM010000121 DAZSNM010000000
VERSION     DAZSNM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11986
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.121"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..568
                     /locus_tag="ACHW42_002719"
     CDS             <1..568
                     /locus_tag="ACHW42_002719"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="penicillin-binding protein 5; removes C-terminal
                     D-alanyl residues from sugar-peptide cell wall precursors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR8944474.1"
                     /translation="EFTFNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATE
                     GQMRLISAVMGGRTFKGREAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRA
                     SLGVDKDVYLTIPRGRMKDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPL
                     VVLQEIPEGNFFGKIIDYIKLMFHHWFG"
     gene            679..942
                     /gene="ybeD"
                     /locus_tag="ACHW42_002720"
     CDS             679..942
                     /gene="ybeD"
                     /locus_tag="ACHW42_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR8944475.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            1043..1684
                     /gene="lipB"
                     /locus_tag="ACHW42_002721"
     CDS             1043..1684
                     /gene="lipB"
                     /locus_tag="ACHW42_002721"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR8944476.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            1943..2896
                     /locus_tag="ACHW42_002722"
     CDS             1943..2896
                     /locus_tag="ACHW42_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR8944477.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            3105..4070
                     /gene="lipA"
                     /locus_tag="ACHW42_002723"
     CDS             3105..4070
                     /gene="lipA"
                     /locus_tag="ACHW42_002723"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR8944478.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(4171..4374)
                     /gene="tatE"
                     /locus_tag="ACHW42_002724"
     CDS             complement(4171..4374)
                     /gene="tatE"
                     /locus_tag="ACHW42_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR8944479.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(4503..5291)
                     /locus_tag="ACHW42_002725"
     CDS             complement(4503..5291)
                     /locus_tag="ACHW42_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR8944480.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            5384..5767
                     /gene="crcB"
                     /locus_tag="ACHW42_002726"
     CDS             5384..5767
                     /gene="crcB"
                     /locus_tag="ACHW42_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR8944481.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(5821..6030)
                     /gene="cspE"
                     /locus_tag="ACHW42_002727"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(5821..6030)
                     /gene="cspE"
                     /locus_tag="ACHW42_002727"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR8944482.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(6205..6765)
                     /gene="pagP"
                     /locus_tag="ACHW42_002728"
     CDS             complement(6205..6765)
                     /gene="pagP"
                     /locus_tag="ACHW42_002728"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR8944483.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            7354..8739
                     /gene="dcuC"
                     /locus_tag="ACHW42_002729"
     CDS             7354..8739
                     /gene="dcuC"
                     /locus_tag="ACHW42_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR8944484.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(8780..9121)
                     /locus_tag="ACHW42_002730"
                     /pseudo
     CDS             complement(8780..9121)
                     /locus_tag="ACHW42_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(9459..9968)
                     /locus_tag="ACHW42_002731"
     CDS             complement(9459..9968)
                     /locus_tag="ACHW42_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR8944485.1"
                     /translation="MKSANEINQWMTLKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            10140..10376
                     /locus_tag="ACHW42_002732"
     CDS             10140..10376
                     /locus_tag="ACHW42_002732"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8944486.1"
                     /translation="MSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVRPWP
                     KGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            10351..11982
                     /locus_tag="ACHW42_002733"
                     /pseudo
     CDS             10351..11982
                     /locus_tag="ACHW42_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         3138 a         2812 c         2787 g         3249 t
ORIGIN      
        1 agaatttacg tttaacggta ttcgccagct gaaccgtaac ggcctgttat gggataacag
       61 cctgaatgtc gacggcatca aaaccggaca cactgacaaa gcaggttaca atcttgttgc
      121 ttctgcgact gaaggccaga tgcgcttgat ttctgcggtg atgggtggac gtacttttaa
      181 aggccgtgaa gccgaaagta aaaaactgct aacctggggc ttccgtttct tcgaaaccgt
      241 taacccactg aaagtaggta aagagttcgc ctctgaaccg gtttggttta gtgattctga
      301 tcgcgcttcg ttaggggttg ataaagacgt gtacctgacc attccgcgtg gtcgcatgaa
      361 agatctgaaa gccagctatg tgctgaacag cagtgaattg catgcgccgc tgcaaaagaa
      421 tcaggtcgtc ggaactatca acttccagct tgatggcaaa acgatcgagc aacgcccgct
      481 ggttgtgttg caagaaatcc cggaaggtaa cttcttcggc aaaatcattg attacattaa
      541 attaatgttc catcactggt ttggttaaaa attgaacact tgaaagtgta atttccgtcc
      601 ccatatacta agcatcagta aaaaaactcc cgccttctgg cgggagttgc tatttaatta
      661 cgttacgccg gagctgacat gaaaaccaaa cttaacgaac tgcttgaatt ccctactcct
      721 tttacttaca aagttatggg gcaggcgtta cctgagctgg ttgatcaggt ggttgaagtg
      781 gtacagcgcc atgcgccagg tgactacacc ccaacggtaa aaccaagcag caaaggcaac
      841 taccactcgg tatctatcac tatcaacgcc actcatatcg agcaggttga aacactgtat
      901 gaagaactgg gcaaaatcga tattgtccgc atggttctgt aactcgcttc tccgttaccc
      961 ggctcctggt cgggtaactc ccctctcctc gctgtgatat acttttcccc cacttttact
     1021 cattctccac ggagatgccg ttttgtatca ggataaaatt cttgtccgcc agctcggtct
     1081 tcagccttac gagccaatct cccgggctat gcatgaattc accgataccc gcgataatag
     1141 tacccttgat gaaatctggc tggtcgagca ctatccggta ttcacccaag gtcaggcagg
     1201 aaaagcggag cacattttaa tgccgggtga tattccggtg atccagagcg atcgcggtgg
     1261 gcaggtgact tatcacgggc cgggacaaca ggtgatgtat gtgttgctta acctgaaacg
     1321 ccgtaaactc ggtgtgcgtg aactggtgac cttgcttgag caaacagtgg tgaataccct
     1381 ggctgaactg ggtatagaag cgcatcctcg ggctgacgcg cctggtgtct atgtcgggga
     1441 aaagaaaatt tgctcactgg gtttacgaat tcgacgcggt tgttcattcc acggtctggc
     1501 attaaacgtc aatatggatc tttcaccatt tttacgtatt aatccttgtg ggtatgccgg
     1561 aatggaaatg gctaaaatat cacaatggaa acccgaagcg acgactaata atattgctcc
     1621 acgtttactg gaaaatattt tagcgctact aaacaatccg gacttcgaat atattaccgc
     1681 ttaattccat acatcaatgg cccaatttac actgggtcat tactcataat acctgctaat
     1741 gctttacatc tccttcgcgt ttgtatattt tctttatcac ttgcagaaat gcggtgccac
     1801 gccttgctcc gtaactcacc ccattgatta acctattgaa atggttgatt atggtgagca
     1861 aaagcgttcc gttccaatta gacataagat tattgtaatt ttttaaatat tatttttcca
     1921 tgaataaaat tggagtaagt gcgtggatag taataatcaa attgaaccct gtttaagcag
     1981 aaagtcgtca gaaggtaaac cacaaatatt tacaacctta cgaaatattg atcttaacct
     2041 tctgactatt tttgaagctg tatatgtaca taaagggatc gttaatgcag cgaaagtgct
     2101 taatctgacc ccctcggcaa tcagtcagtc tattcagaaa ctgcgcgtta tattccctga
     2161 cccattgttt attcgcaaag gccagggtgt cactcctacc gcatttgcga tgcatctaca
     2221 tgagtatatc agtcagggcc ttgagtccat tcttggcgcg ctggatatcg aaggaagcta
     2281 tgataagcaa cgaacgataa ctattgcgac cactccctcg gtcggagccc tggtccttcc
     2341 tgtcatctat cgggcgatta aaactcacta tccgcagctt ttactgcgca acccgcccat
     2401 cagcgacgcg gaaaaccaac tcagtcagtt tcaaaccgat ctcatcatcg ataacatgtt
     2461 ttgcaccaat cgtacggtgc aacatcatgt tctgttcacc gacaatatgg tgttaatttg
     2521 ccgcaaggga aatccactac tctctttaga agatgacaga gagactatcg acaacgctgc
     2581 gcatgtactt ctgttaccgg aagggcaaaa tttcagcggt ctgcggcaga gagttcaaga
     2641 gatgtttccg gaccggcaaa tcaatttcac cagctacaac attttgacaa tcgctgcact
     2701 ggttgccaac agtgacatgt tagcgattat tcccagccgt ttttataacc tgtttagtcg
     2761 ttgctggccg ctggaaaaac tgccttttcc gtccttaaat gaggagcaaa tagatttctc
     2821 tatccactac aacaaattca tcctgcgtga tcccatcctg cacggagtaa tagatgttat
     2881 ccgtaatgca ttttgaaaaa gtattgttac agccagatca cagcgcagaa cgataaaatg
     2941 gcacaatcca acaacaattt tacattttcg cgaccgcttt ggctgctttc aggtccgttt
     3001 caatgatata ctgccagtcg ttaattcaaa aatagttgat aattacaaca atctattgaa
     3061 ttgaaacgct ttccttcgta attcgcaact ggaacacgca cgctatgagt aaacccattg
     3121 tgatggaacg cggtgttaaa taccgcgatg ccgataagat ggcccttatc ccggttaaaa
     3181 acgtggcaac agagcgcgaa gccctgctgc gcaagccgga atggatgaaa atcaagcttc
     3241 cagcggactc tacacgtatc cagggcatca aagccgcaat gcgcaaaaat ggcctgcatt
     3301 ctgtctgcga ggaagcctcc tgccctaacc tggcggaatg cttcaaccac ggcacagcaa
     3361 cgtttatgat cctcggcgct atttgtaccc gccgttgtcc gttctgtgat gttgcccacg
     3421 gtcgcccggt agctcctgat gccaatgaac cagtgaaact ggcgcagacc attgccgata
     3481 tggcgctgcg ttatgtggtt atcacctccg ttgaccgtga tgacctgcgc gatggcggtg
     3541 cccaacactt tgcggattgt attactgcca ttcgggaaaa aagcccgcaa atcaaaattg
     3601 aaactctggt gccggatttc cgcggtcgaa tggatcgtgc tctggatatt ctgactgcaa
     3661 cgcccccaga tgtgttcaac cataacctgg aaaacgtacc gcgtatttac cggcaggtac
     3721 ggcctggcgc agattacaac tggtcgctga agctgctgga acgctttaaa gaagcgcatc
     3781 cggaaatccc gaccaagtct ggtctgatgg tgggtctggg tgaaaccaat gaagaaatta
     3841 ttgaggtaat gcgcgacctg cgccgtcatg gtgtgacgat gttaacgctg gggcaatatt
     3901 tgcagccaag ccgccatcac ctgccggttc aacgttacgt tagcccggat gagttcgacg
     3961 aaatgaaagc cgaagcgctg gcgatgggct ttacccatgc tgcatgcggt ccgtttgtcc
     4021 gctcttctta ccacgccgat ttgcaggcga aaggaatgga agttaagtaa tcctgtaata
     4081 attacttaca tctgtctgat aaaaaacccg ctttttgaag cgggtttttt gtatcgaaca
     4141 agatattgag ggagcgtcct gctcgccacg tcactcttta tgagagagct tttcagcctg
     4201 aagatcaacg tctgcgcctt ttttcgccgc agcatcgtca tcattcatcg ccttcttgaa
     4261 ccctttaatg gccgctccaa ggtctccgcc cagcgtacgt aacttcttag tcccaaacag
     4321 cagaacgacc agcgccgcaa ctaccagcag tttggtaata ctaatctcac ccatagatac
     4381 cttcttgaca tataaccggc aacagaacgc gccttaaaaa ccagagaata ttaacggtca
     4441 ttcgacgcgc gcacacaata gcaatctgta acaaggtgaa tcaagcgccg agtaaaaaaa
     4501 catcataata attgcggcgg cgcaaagcga cggttgttta agatgggcag ttgcgcgcgc
     4561 acctgacgca cacgttcggg cgtcacttcc gccataatga gtgcaggcat ttctgacgct
     4621 gccgcaatgg tgacgccaaa gggatctata atccggcttt gcccgatatt tttgttcccg
     4681 cactcccccg ccgccaccat ataacaggtg gtatccagcg cacgagcggc aagcaacgtt
     4741 gaccagtgat gctctttgag cggcccacga acccaggcgg caggaagtac caggatttca
     4801 gctccctgta aagcctgtgc cagcgccagc tctggaaagc gtaagtcata acaggtcatc
     4861 agaccgacct tcatcccttc cacctccagt aacggagcta tttcattacc agcatcaaca
     4921 tggcgtgatt cctgaatggc aaatgcatca tagagatgca gtttggcata acgggcgacg
     4981 atgtttcctg cctgaagtgc caccagcata ttccatgcgc gccccggcgt tgaaggaaga
     5041 tgaatcgtca gaattgtcgt catcatgtta cgtttacttt ctcgccgtaa acgtccgagg
     5101 aattcgcctt ccagcagctg tgctgattta accgatagat ctgcatcatg atcatcgcgc
     5161 gccagcaatg cttccggcag gacaaacagc gatacgtcgt tttccgccgc ctgcgccatc
     5221 aacgaggcac aaatctcagc gttcttttcc cacacagatg taacagcaaa ctgtcctgca
     5281 gcaaccagca ttgccacctc ctgtcagagt cgtaaaattc gccaacgacg attattcggc
     5341 gttacactta tcactcatac aaatcaaata gcaggatttt gcagtgttac aacttctttt
     5401 agcagttttt attggcggtg gtacgggaag cgtagcgaga tggctgttaa gtatgcgatt
     5461 taacccgctg catcaggcga ttccgttggg gacgctggca gcaaacctga ttggggcatt
     5521 catcatagga atgggattcg cctggttcag caggatgacg aacattgatc cagtgtggaa
     5581 agtattaatc accaccggat tttgtggcgg tctaacaacc ttctcaacat tttcggcaga
     5641 agtggtgttt ttgttacaag agggccgctt tggctgggca ttactgaacg ttttcgtcaa
     5701 ccttctgggg tcttttgcca tgaccgcact ggcattctgg ctgttttcgg cctcaaccgt
     5761 acactaaaga ccaaaaaaaa cccgctgatt aagcgggttt tgaattcttg ctgacgtatc
     5821 ttacagagcg attacgtttg cagcagaagg gcctttggca ccgttagtga tttcgaactc
     5881 tacgcgctga ccttcagcaa gagttttaaa accattagtc tggattgcag agaagtgtac
     5941 gaacacgtct ttgctgccgt cttccggagt aatgaaaccg aatcctttgg actcattaaa
     6001 ccacttaacg ttacctttaa tcttagacat caaaattacc tttacatgaa aaatagacac
     6061 aaatgctgtg tcggttatca gtacaccaat tgtggtacgc tttgtccagt gcaactttac
     6121 taaaaagtga taaaagtcgc gttatttttg atgttgctta ttttagttat tgattttaaa
     6181 gaagttacta aaacttcatt tgtctcaaaa ctgaaagcgc atccaggcaa agtacacatt
     6241 gccattgttg taggtacccg gaatgtaggt catctgaaaa gtcactgggc cataacccac
     6301 ggaggccaat ggcagtagaa ccgggagagg gatgtaattc cagttatcgc gtgccgttac
     6361 gccagcggtg aatcccagac ctaaatgaaa attttcatcc gccagcggtc gccaggtact
     6421 ttcccatccg tatccggcaa tcggttccca tttgttccac gagtccttaa atgccatggc
     6481 atacaggcca tgccagtttc ctttttcatc ccaacgcgac aggccaaaac cgccacccca
     6541 cggtcgctcg ttatagcgat cggttttttc tttgtcgtaa gcgaaacgtg catgccaggt
     6601 gatggcagga atatataaat cataatgttc aggctgttgc caggtttgtg caatattttc
     6661 tctaaacgtt gtcatcctct catctgcgtt agcaaaaact ttaccaacgc tgattaactg
     6721 aataaaaaca aaggaaaaga tagcgacata tttactcacg ttcatttgtg accataaaac
     6781 atttatcaaa aatctactac tagcatagca aagctacaat taacataacc ttaatagacc
     6841 caacataaag aataatctga atagctgctc tatttatttc cgcttgttca agttaaatat
     6901 ttccgcgcat cgattaaaga tgatgcgtcg ttttctgcga tgggaatagt caaaaaagaa
     6961 aaaccaagtc ttttttgatg acaaaatgca atcaaggaaa aattatttta tttttttaac
     7021 gagatacaac aatcatctta acgaagtata taatatatat cgtcaaccca tgctcatatt
     7081 agattatttt ttgagcgcac agccgcagct aacaccattg caattaacaa atttgcatca
     7141 atccaccatc aatttacaca cattattatg tgataattgc caaccgctaa atatgcgttt
     7201 tgttatctat gtataaaaac agcaacttca atgtcttaat ggcagttttt cttgatttta
     7261 atcagcattc atcgccaatt tattgggcat attttttcct taagctttag gaagttttta
     7321 ttatttactt tgggcctgga gacaggaaaa attatgctga cattcattga actccttatt
     7381 ggggttgtgg ttattgtggg tgtggctcgc tacatcatta aagggtattc cgccactggt
     7441 gtgttatttg tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg
     7501 ttaccgtcca gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa
     7561 atattactaa tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggcttt
     7621 gccgcttaca tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca
     7681 ttgcagtata ttaactcccc ttacctgctg atgattgccg cctattttgt tgcttgtctg
     7741 atgtcactgg ccgtctcttc cgcaaccggt ctgggggttt tgctgatggc aaccctgttt
     7801 ccggtgatgg taaacgttgg tatcagtcgt ggcgcagcag ctgccatttg tgcctccccg
     7861 gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca ggcttccgaa
     7921 atgtcgctga ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc
     7981 ggcatggcga tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct
     8041 catgaaatgt tagatgtcag tgaaatcacc accactgccc ccgcgtttta tgccattttg
     8101 ccgttcacgc cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac
     8161 atcatcacta ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc
     8221 tttaataccc agaaagtttt ctctggtctg gaagtggctt atcgcgggat ggcagatgcg
     8281 tttgctaacg tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc
     8341 atcggcttta ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc
     8401 ctgatgctgg tattggtgat tctgaccatg ctggcggcag tcacgaccgg ttcaggcaat
     8461 gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac
     8521 ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc
     8581 gtttctggcg tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta
     8641 aaacgcacct cggtgccggt gcttgttggg ctagtgattg ttatcgttgc tacagagctg
     8701 atggtgccag gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt
     8761 ttcctccggc tttatcatgt cacccactgt ggtatatgcg ttgtggtctg ccaactttgc
     8821 cgtgaacaat ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg
     8881 cagtggtgcg gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag
     8941 gctctttaaa cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggcagtt
     9001 cgtctttggg ttcaccgcga gcataggcat taaacatctc atcaatttgc ttctggctgg
     9061 cgctatcaat actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca
     9121 gtgtattgat tcacttgaag tacgaaaaaa ccgggagaac attggattat tcgggatctg
     9181 atgggattag atttggtggg gcttgcaagc ctgtagtaca aattttaggc gttaaccaat
     9241 gaaacgcgaa agatagtaaa aaattgcttt tgtttcattg aaaaaacgaa aaacaaaaac
     9301 actataaatc atttcaatca tagcttcaaa aaacgttcaa aaccgataac aaccaggctg
     9361 tcatcaaatg actcatatca caaatcagct catgccgttt aggtatgtta catgtgtgat
     9421 tatgtgaggt gaagtatgtt ttagctggct catggttgtt atacggcttt tttcgcatct
     9481 tgtggttcca acaaaggagc tacaggctct tcctgtttat gaacaccata cattgacaaa
     9541 atctcttggc gagtttctgg aggtaattta tgcagcgcca aagctgtttg tagtgtttgg
     9601 cgatcaatac ccagcgttgc caatagggtg tcaacggaga tggaggggcg ttcttgcatt
     9661 aatccggaaa gcccatattt agccatgttt agctctatct ctcggacttc attttctcct
     9721 agtttattga ttaacaggga gaacattttc aatgtatcag cattcatttc ttcgatagca
     9781 attttttgca tagccggtat tgccgggata tattcaaccc caggcccgcg acgcccttct
     9841 tgtgaacgct tcggccatcc ttctttcttt gctcgtttat gcaccccctg tattgtccca
     9901 ggcattccat ccagttcagt aatttgtttg agtgtcatcc actggtttat ctcatttgcg
     9961 cttttcataa actaagttta ccagatttat ctatataaaa atcaatggat tacaaaataa
    10021 accacattaa ctgatataaa ccggtttggt ttatttggta atgtataaaa cagataatca
    10081 agacgaatta tttgttttgg taacccttta tcatacgcaa tatggttggt gtgcgaaaga
    10141 tgagtaacga ttggcatcgg gccgacgtga ttgcggccct caaaaaaaca ggaacccctc
    10201 tggcaaaatt gtccagacgt tcaggttatt caaccgggac gcttgcaaat gcattagtca
    10261 ggccatggcc taaaggtgaa cgcattattg ctgacgcctt aaacatgaaa cctgaggaaa
    10321 tttggcctag tagatatcta agggatcaca atgagagagt ggatatcagc aattgaatgc
    10381 atgggtttat ctggtttacc tggttcatta atgggggttc acaaaagagc tcagagagaa
    10441 ggatggctca agagaagaag agaaggaatt agaggggttg cttacgaata ccatatatct
    10501 tctcttccta acattgctcg agctgaagtt ttgctccaac aaggaaaatt taaaaccagt
    10561 cagggatgtt ttgaaatcgc ccgccccacg ctggaagctc atgattatga tcgtgaggca
    10621 ctatggagca aatgggataa agccagcgat tcccagcgca gcctggctga aaaatggttg
    10681 ccctcaattc aggccacaga cgaaatgctg aaccagggga tttcaacgaa aacggctttt
    10741 gcgaccgttg cagggcatta ccaggtcagc gcatccactt tgcgggacaa gtattaccag
    10801 gtacagaagt ttgcgaagcc tgactgggcg gctgcacttg ttgatggacg tggagcatcc
    10861 cgtcgcaatg ttcacaaaag tgaatttgac gaggatgcct ggcagtttct gattgcagat
    10921 tatctgcgac cggaaaaacc cgccttccgc aaatgttatg agcgtctgga actggcagcc
    10981 cgcgagcatg gctggagtat tccctcccgt gccacggcct ttcgccggat tcagcaactg
    11041 gacgaggcaa tggttgttgc ctgtcgtgaa ggtgaacatg cactgatgca tctgataccg
    11101 gcacagcagc gaactgtgga acacctggac gccatgcagt ggatcaacgg cgacggttat
    11161 ctgcataacg tctttgtacg ctggtttaac ggtgatgtga tccgtccgaa aacatggttc
    11221 tggcaggatg tgaaaacccg aaaaattctg ggctggcgct gcgatgtgag cgagaacatt
    11281 gattcaattc gcctctcgtt catggatgtt gtgactcgct acagtatccc ggaggatttt
    11341 cacatcacca ttgataacac ccgtggtgct gcgaataaat ggctgacggg aggcgcgccc
    11401 aatcgctacc gctttaaggt aaaagaggac gatccaaaag gactgttttt actgatgggt
    11461 gcgaaaatgc actggacaag cgttgttgcc ggtaaaggct ggggccaggc aaaacctgtt
    11521 gaacgtgctt tcggtgttgg tgggcttgag gaatacgttg ataagcatcc ggcactggct
    11581 ggcgcatata cggggccaaa tccgcaggca aaacctgata actatggcga ccacgctgtt
    11641 gatgcagagc tgtttctgaa aacctttgcc gaaggtgtgg cgatgttcaa tgccagaaca
    11701 ggccgtgaaa cagaaatgtg cgggggcaaa ctctcgtttg atgatgtttt cgagcgtgaa
    11761 tacgccagaa cgattgtgcg taagccaacc gaagaacaaa aacggatgct gttactgcct
    11821 gccgaggcgg tgaacgtttc acgcaaaggc gagtttacgc ttaaagttgg cggctccctt
    11881 aaaggcgcga aaaacgttta ttacaacatg gcattaatga atgccggagt gaaaaaagtt
    11941 gtggtcagat ttgatccaca gcagttacac agcacggttt attgag
//
LOCUS       DAZSNM010000122        11924 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZSNM010000122 DAZSNM010000000
VERSION     DAZSNM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.122"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..2251)
                     /locus_tag="ACHW42_002734"
     CDS             complement(<1..2251)
                     /locus_tag="ACHW42_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HFR8944487.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTEGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFRDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTLYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="ACHW42_002735"
     CDS             complement(2275..2898)
                     /locus_tag="ACHW42_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFR8944488.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="ACHW42_002736"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="ACHW42_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="ACHW42_002737"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="ACHW42_002737"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR8944489.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEYIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /gene="ubiG"
                     /locus_tag="ACHW42_002738"
     CDS             7506..8228
                     /gene="ubiG"
                     /locus_tag="ACHW42_002738"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR8944490.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..11924)
                     /gene="yfaL"
                     /locus_tag="ACHW42_002739"
                     /pseudo
     CDS             complement(8356..11924)
                     /gene="yfaL"
                     /locus_tag="ACHW42_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2760 a         3306 c         2942 g         2916 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagacctt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtat tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt tataaaccgc caagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccagcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag ctatccagcc aggccccctg
     8881 cttctgatta ccgtgctgat accagcttga ggtcagccca acggcataac cgtggttctg
     8941 gttatcggcg cgagttccgg tcatattcga gcggctgtcg ccctgattat cactgtagcc
     9001 accaaccgcc ccaagcatcc actcgccatc cgtgccccag cgcccgctaa acaagccgcc
     9061 gctaagctgc accgtagaag tgtcttcctg ctgagccagt tgccctgccg ccgtgtaatg
     9121 ataacgtccg ccgataacac gtaaattcag cgtctgacca tcgccaccag cgtgatcgcg
     9181 tcgctccatc ataaacgcct gatttgccgc ccgcaggtta ttgagataac cgccaacttt
     9241 ggcattcagc accggctggt aagcaggcgt aggggccggg tcgggggttg ggtcaggatc
     9301 cggcgtggga tcaggatcgg gagtcgggtc tggctcaggt gacgatggcg gcgttacttc
     9361 ttgcgatcgc agataccagt cgttgttatc ttccactagc gtgtagtcat acgctcccat
     9421 gttgacgtag ccactgccag ccagactgaa ctgtgcattg ttttgaaact gcgtgggatc
     9481 agctgcgaaa tcaaccactt taatgcctgt cgatgtcggc tcaccaatcc ctgtaatgga
     9541 gttaaccacc acagttgtgt tgccagcagt attaccgttc atcaccaatt gatcgcttac
     9601 cgagtcatcg ccgtttaatt cgctgtcgaa tagtagcgta ccgccgccgg tataatcacc
     9661 gtttaccgtc agcgtatcgc cagcgacgcc attttgcagg ctaaccgcac cggacgtatt
     9721 ggtcagatca ccattaaccg tcacgtcagc aagataggtc aggctgtcag tttcaaggtt
     9781 ggtattaagc tcgcctgtca acgtcacgcc atcggtagca tttaccagcg tcccatcgcc
     9841 gttaaatagt gaggcattaa gggcgacgga agtatcctgc ccggtcagac gcaaaaccgt
     9901 accatcgctg atgtcgatag tgccgctgga agtagcatca attgactgaa tatcctgatc
     9961 cgcgccagta acgaacgttg ccccatcacc aacccacagt gacgaagcat aaggaaagat
    10021 atccgcaaca tcgcctttca gggtgccttc ttcgacacgt accgccgact gcgttgtacc
    10081 gctggcggtc agctccagcg tacccgcccc cgttttagta aatgtgctac cctcatcctg
    10141 atgctgcccg ctgctgtcag ccatcagtgc gccccactgc gtgtctaccc ccgcatcaac
    10201 tgccacttca ccgtcggcgc gcatttcaat atcacgccca tgctcagtcg tcgtagagcg
    10261 agtatcgaca ttggcagtaa tctccatcac gctttcttgt tacttatcgg taaagataac
    10321 ctggcggtta tagtgggtat ctccaagctg cgagttgtcg ctcaccatca gcgtaccgga
    10381 ggcgatttgt gttgtgccca aataactatt attgtttgcc agacttacct gcccgtcgct
    10441 cgcatcgacc accacgtaga cgccatcttt cccgtcgccg ccgatatgca aattgtcgcc
    10501 acctgcaaga tctaccacat cctgactgcc gataaccgtt ccgctgctgc cactgacttc
    10561 aaggccatca ttgtatgatg tgccgctctg ccaagtggag aaatcagaga gatcgagcac
    10621 accgccgttt aacacgatcg actgcggatc gtcctggaga gcggtaagat ctgccgcgtc
    10681 gccttccagc gaaagcaccg caccatcatc aacgactata tcgccggtta gcgccatcga
    10741 ctgcgcccct gccagcacgt agctgccgtt ttgcgcaatg gtgagctgac cagcaccttc
    10801 gatgatgcca gcaaaactgc cctgattaac agtaacgttg ccaccagcat cgatatttaa
    10861 agtgccattc tgaaagcccg tcaatgcgtg cacaaaagtt tgttgggtcg agccaacgtt
    10921 tagctcagcc tgattctgat actgatcaat actccctatc gtcagaccgt agcagtcttg
    10981 cggatcgtcc tggcaatgcg tatcgccgac attcatcagg gagttgctgc gacccagggt
    11041 aacttcaccg ttttcaatct gcatctcacc agtaaagtca ttgttatctg cattaagtac
    11101 cagatcgccg gaacctgttt tggtgattaa cccggtacca gcaatagagt caacagctcc
    11161 gtcattctct gtattgccga taaccagcgt ttttccgtcg gcaatatcaa aggtaacttc
    11221 acttaagccg agatacataa agccacccgc cgcagtggag ggaccatctc catagcctgc
    11281 tgcgctattg ttctcatcga ctaacacgcc gccgttctgg ctgtagctgt catcaacaga
    11341 aatatcaata agatagggag ccgtcgcgct attggtatat atcgccccgc cataaccttc
    11401 ggctgtgtta tttgtaaagg cggtgttatt tattatcgta taacctgaag gatgcttgct
    11461 gtcgctatta ttatcggtaa catcgattgc cccgccatcg ccatcactgt aacttgttga
    11521 tgtatatgcc tggttattgt taaaaacatc atcacttaaa tagacgtcat tattaatggt
    11581 ataaattgcg ccacctttgc catcattagc gatattattg atggcgttag tgacacgtaa
    11641 atcgacggca ccagtatcat tagtaccaga agaatagatt gcgccaccat agccgcctgc
    11701 gacgttaccg gaaaaaataa catcagtaag attcagcgtt gagttttctt tagcaaatat
    11761 ggccccgccg ttattatatt ctcctgtaac ggtgttattg gcaaacagag tcattccagt
    11821 ttcattttct ggtaatagtg aaaattccgc tccttgttgc aaaaatacgg ccccaccgct
    11881 ggcattattg gtcatatccg aaaaaaccag ccattgccca tcgg
//
LOCUS       DAZSNM010000123        11904 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZSNM010000123 DAZSNM010000000
VERSION     DAZSNM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11904
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.123"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(140..880)
                     /gene="cysQ"
                     /locus_tag="ACHW42_002740"
     CDS             complement(140..880)
                     /gene="cysQ"
                     /locus_tag="ACHW42_002740"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR8944491.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            1070..3013
                     /gene="cpdB"
                     /locus_tag="ACHW42_002741"
     CDS             1070..3013
                     /gene="cpdB"
                     /locus_tag="ACHW42_002741"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR8944492.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3139..3519)
                     /locus_tag="ACHW42_002742"
     CDS             complement(3139..3519)
                     /locus_tag="ACHW42_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR8944493.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3608..4468
                     /gene="qorB"
                     /locus_tag="ACHW42_002743"
     CDS             3608..4468
                     /gene="qorB"
                     /locus_tag="ACHW42_002743"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR8944494.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            4577..5542
                     /locus_tag="ACHW42_002744"
     CDS             4577..5542
                     /locus_tag="ACHW42_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR8944495.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5650..6312
                     /gene="ytfE"
                     /locus_tag="ACHW42_002745"
     CDS             5650..6312
                     /gene="ytfE"
                     /locus_tag="ACHW42_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR8944496.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6357..7769)
                     /gene="cycA"
                     /locus_tag="ACHW42_002746"
                     /gene_synonym="dagA"
     CDS             complement(6357..7769)
                     /gene="cycA"
                     /locus_tag="ACHW42_002746"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR8944497.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(8077..8697)
                     /gene="fklB"
                     /locus_tag="ACHW42_002747"
     CDS             complement(8077..8697)
                     /gene="fklB"
                     /locus_tag="ACHW42_002747"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR8944498.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8916..9461
                     /locus_tag="ACHW42_002748"
     CDS             8916..9461
                     /locus_tag="ACHW42_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HFR8944499.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(9445..10092)
                     /locus_tag="ACHW42_002749"
     CDS             complement(9445..10092)
                     /locus_tag="ACHW42_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR8944500.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            10304..11095
                     /locus_tag="ACHW42_002750"
     CDS             10304..11095
                     /locus_tag="ACHW42_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFR8944501.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            11105..>11904
                     /locus_tag="ACHW42_002751"
     CDS             11105..>11904
                     /locus_tag="ACHW42_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR8944502.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         3012 a         3085 c         2993 g         2814 t
ORIGIN      
        1 aacgctcatt attaaaatgt aacttactag caattgaatt tttgcacttt aaatacaatc
       61 tctttttatt tataactttt taggttggga taaatcagga tggcagaatc aggaaataca
      121 ctgtttctgc catctgaatt tagtaaatgg acactctgaa ccccggattc aggaacgact
      181 cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg aacgtgcgct ccggcagctg
      241 cagccacagc atgtccggcg gcggtgtccc aaatattcgt tggtccgaag cgcgggtaca
      301 gctgcgcctg tccttccgcc accaggcaga atttcagcga agagccgatg gacgtggtct
      361 gatgttcgcc aagctgttgc agatactctt tcagctccgc atctgcatgg gaacggctga
      421 taaccaccag cggcgggcgc gcatcgcgga cctgaatcag cttgcgcaca ccgcactctt
      481 ctttccaggc cttgccttct gccgcgctgt acattacgtt cattaccggc gcatacacca
      541 cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt aacggtgaat tcgccattac
      601 gtttaataaa ctctttagta ccatccagcg ggtctaccag ccagtaacgc tgccagtgct
      661 gacggacttc ccaaccggga ggatcttctt cagaaaggac cggaatatcc ggtgccagcg
      721 tacgtaaacc gtccatgata acggtgtgag cggcaatatc cgctgccgtt accggagaat
      781 tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc gtagacctgc ataatggcat
      841 cgcctgcatt ccgtgcaagc tggcatactt gatctaacat ttctccacct cgtctctgtg
      901 agcggtgtta acttattgtt ttacttatac cctatcgtta atgaatgcgc caactgtgat
      961 agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc actgttctat aagtaaggcg
     1021 tttattctcc cttttctttc gtattcccga tgataaaagg atgtccctga tgattaagtt
     1081 tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg aatgcagcga cggtcgatct
     1141 gcgtatcatg gaaaccactg atctgcatag caacatgatg gatttcgatt attacaaaga
     1201 caccgccacg gaaaaattcg gactggtacg tacggcaagc ctgattaacg atgcccgcaa
     1261 tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg attcagggga gtccgctggc
     1321 cgattacatg tcggcgaaag gattaaaagc aggtgatgtt catccggttt ataaggcgct
     1381 gaatacgctg gattatacgg tcggtacact cggcaatcat gaatttaact acggtctgga
     1441 ttacctgaaa aatgcgctgg cgggagcgaa attcccttat gtaaatgcca acgtcattga
     1501 cgccagaacc aaacagccaa tgtttacacc gtatttaatt aaagataccg aagtggtcga
     1561 taaagacgga aaaaaacaga cgctgaagat tggctatatt ggcgtcgtgc cgccgcaaat
     1621 catgggctgg gataaagcta atttatccgg aaaagtgacg gtgaatgata ttaccgaaac
     1681 cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc gatgtcattg tcgttctggc
     1741 gcattccggg ctgtctgccg atccgtataa agtaatggcg gaaaactcag tttattacct
     1801 cagtgaaatt ccgggcgtta acgccattat gtttggccat gctcacgccg ttttcccggg
     1861 taaagatttt gctgatatcg aaggggctga tatcaccaaa ggcacgctga atggtgttcc
     1921 ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg gtcgacttac aactcagtaa
     1981 tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa gcacggccga tttacgacat
     2041 cgccaataaa aaatccctcg cggcggaaga cagcaagctg gtagaaacac tcaaagccga
     2101 tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt aaatctgccg acaatatgta
     2161 tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa gtcgtgaaca acgcgcaaaa
     2221 agcgtatgtc gaacattaca ttcagggcga tccggatctg gcaaaactgc cggtgctttc
     2281 agctgccgca ccgtttaaag ttggtggtcg caaaaatgac ccggcaagct atgtggaggt
     2341 ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt tatctctacc ccaatacgct
     2401 gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg ctggagtgct ccgccggaca
     2461 gtttaaccag attgctccta acagcacgaa accacagtca ctcatcaact gggatggttt
     2521 ccgcacttat aactttgatg ttattgatgg tgtgaattat cagattgatg ttacccaacc
     2581 cgcccgctat gacggcgagt gccagatgat taatgccaat gcagagcgga ttaagaacct
     2641 gacctttaat ggcaagccga ttgatccgaa cgctatgttc ctggttgcca ccaataacta
     2701 tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc catatcgctt ttgcttcacc
     2761 ggatgagaac cgctcggtgc tggcagcgtg gattgctgat gagtcgaaac gtgcggggga
     2821 aattcatccg gcggcagata acaactggcg tttagcaccg atagctgccg ataagaaact
     2881 ggatatccgt ttcgaaactt ccccgtcaga taaagccgca gcgtttatta aagagaaagg
     2941 gcagtatccg atgaataaag tcgcgaccga tgatatcggg tttgcgattt atcaggtgga
     3001 tttgagtaag taaaacactt ctttttcggc ctataaatca accgcatccg gcatttattg
     3061 gcggatgcga tgctgccgta tcttatccac cctacaagcc atgaaccgta gaccggataa
     3121 gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc accgccaaca cctcgggcaa
     3181 attcaactca atccagtccg ccagtgcagc aaccttttcg ctcacctgct cgcccagcgg
     3241 cgtgaggcta tattccacat gcggcggcac caccggatac gcgatacggt taagaaaacc
     3301 atcctgttct aacgcctgta acgactgcgc aagcatcttt tcactcaccc caccgatttt
     3361 gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc gccaccagaa tcaacacccc
     3421 ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg cactgttccg caaagagatt
     3481 accctctttc agttgttgtg acaggctaac ctgactcatt tcatacttac ctttttgcac
     3541 gtacttacta aaagtaagtt taggtgttag cgtatttaaa cacaagacaa aacgatggag
     3601 acttcccatg atcgctatta ctggtgccac tggccaactt ggtcactata ttattgaatc
     3661 cttgatgaaa acggttcctg ccagccaaat agtggctatc gttcgtaatc cggcaaaagc
     3721 ccaggccctg gcagcacaag gcattaccgt gcgtcaggct gactacagcg atgaagccgc
     3781 actgacatct gcacttcagg gagtggaaaa actactgctg atctcttcca gcgaagtggg
     3841 tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca aaggcggctg gcgtgaaatt
     3901 tatcgcttat accagcctgc tacatgcaga tacctccccg ctcggcctcg ccgatgagca
     3961 catcgagacg gagaaaatgc tggctgattc tggcatcgtt tacaccctgc tgcgcaacgg
     4021 ctggtacagc gaaaactacc tcgccagcgc cccggcagca ctggaacacg gcgtatttat
     4081 cggtgcggct ggcgatggca aaatcgcctc agcaacgcgg gcagattatg cgacagctgc
     4141 ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt tacgaactgg cgggcgatag
     4201 tgcctggacg ttgacacagt tagcggcaga gctgaccaaa cagagcggca aacaggttac
     4261 ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg aaaagcgtcg gactgcccga
     4321 cggactggcg gatatgctgg cggattctga cgttggcgca tcgaaaggtg gtctgtttga
     4381 taacagcaaa acgcttagca aattgattgg ctgcccaacg acaacgttag ccgaaagcgt
     4441 aagccatctt tttaatgtta ataactagtt aattaaagtg gcatcctccc gcatcctctc
     4501 tgataatgac gggatgccgg gagcaatcat gtctgcttcc tgaacgtttc ttctgacaga
     4561 ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc ctgttagcag ggttgatgtg
     4621 ggggcttatt tttgtcgggc cgttgatcgt gccggaatac ccggcgatgt tgcagtcgat
     4681 ggggcgttat ctggcgttag ggttaattgc gctacccatt gcctggctgg gacgcgtgcg
     4741 tctgcgtcag ttggcgcgtc gggactggct taccgccttg atgctcacta tgatgggcaa
     4801 cctcatttat tacttctgcc ttgccagtgc cattcaacgt actggcgcgc ctgtttccac
     4861 gatgattatc ggcaccctgc cggtggtgat tcctgtcttt gccaatctgc tttatagcca
     4921 gcgcgacggc aaactcgcgt ggggaaaact cgccccggca ctgatttgta ttggcatcgg
     4981 cctggcgagt gtgaatattg ctgagttaaa ccacggactc cccgattttg actgggcacg
     5041 ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc tgctgggcat ggtatgccct
     5101 gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat ccgatgatgt gggcgacggc
     5161 gcaggcgctg gtcacgctgc cggtttctct catcggctat ctcgtcgcct gttactggct
     5221 gaatatacaa acgccggact tctccttacc ttttggcccc cgtccgctgg tgtttattag
     5281 tctgatggtt gcgatagccg tgctttgctc atgggttggc gcactctgct ggaacgtcgc
     5341 cagccagcga ttaccgacag tgattctcgg gccgctgatt gttttcgaaa cgctggcagg
     5401 tttgctgtac acctttttac tccgccagca aatgccgccg ctaatgacgc tgagcggtat
     5461 cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca aaaccagaaa aacctttaac
     5521 tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta aataagcctc tgctacgtaa
     5581 gggttatagc ttttgcctta aaggtgtatt taaaatacat cttatcttat taagaatgag
     5641 gtatcagcta tggcttatcg cgaccaacct ttaggtgaac tggcgctctc tatccctcgc
     5701 gcttcggctc tgtttcgtaa atatgatatg gattactgct gtggcggtaa gcagacgctg
     5761 gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca ttgaagctga actggcaaag
     5821 cttgctgaac aaccgattga gaaagactgg cgtagcgccc cgctggcaga aatcattgac
     5881 catatcatcg tgcgctacca cgatcgtcac cgcgagcagc tgccggagct gatcctgcaa
     5941 gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg tgccaaaagg gctgacaaaa
     6001 tacctgacca tgctgcatga agagctttcc agccacatga tgaaagaaga gcagatcctc
     6061 ttcccgatga tcaaacaagg catgggcagc caggcaatgg ggccaatcag cgtaatggaa
     6121 agcgagcacg atgaagcggg cgaactgctg gaagtgatca aacacactac caataacgtc
     6181 acaccgccgc cagaagcctg caccacctgg aaagcgatgt ataacggcat taatgaactg
     6241 attgatgacc tgatggatca catcagtctg gaaaacaatg tactgttccc acgcgcccta
     6301 gcgggtgagt gatctaaaag ctggatggca ttgcgccatc cagcatgata atgcggttat
     6361 ttccgcagtt cagcagcccg cttcttacca ataaacagcc agcccaaccc cagcgcgata
     6421 aaccacagcg gggtaaccag cagcgcctgg cgagtgtcat cttccagtgt cagcaacacg
     6481 accacgaaca caaagaacgc catacatacc cagcacatca gcttgccgag cggcatcttg
     6541 tagatcgact tttcatgcag atgaggacgc tgtttgcggt acaccaggta agagcaaagg
     6601 ataatcgtcc agacgaacat aaacagaatc gcggaaacgg ttgtaatcat cgtgaacgcg
     6661 ccaatcacgc taggattcac atacaacatc accacgccgc cgagcagaca gatacacgag
     6721 aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt tagcgaacgc tttcggcgca
     6781 acaccttcct gcgccagacc aaacagcata cggctggtag agaagacacc gctgttagcg
     6841 gaagacgccg cagaggtcag caccacaaag ttgatcacgc tggcagccgc aggcaaacct
     6901 accaacacaa acagctcaac gaacgggctt ttctccggga ctaccgaact ccacggtgtc
     6961 acggacataa tcacaatcag cgcgaagacg tagaacataa tgatacggat cggaatggag
     7021 ttaatcgcgc gtggcagtga tttctccgga tctttggttt ccgcagctgt tgtacctacc
     7081 agctcaatcc ccacgaaagc gaaaaccgct atctggaatc cggcaaagaa gccacttaaa
     7141 cctttcggga accagccgcc gtcattccac aaatgcgcga atgacgcttc cacaccggtc
     7201 ggtgactgaa agtgcatcgc caccatgacc aggccgacga caatcagcga cacgatggcg
     7261 acgattttga tcatcgcaaa ccagaactcc atctcaccga acattttcac ggtggcaagg
     7321 ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga cccagtcgga gagatcgggg
     7381 aaccagaact gagcataagc cgtgatcgcc accacgtctg ccataccggt tacaacccag
     7441 cagaaccagt aagtccagcc ggtgaaatat cccgcccacg gccccagtaa atcggaagcg
     7501 aagtcactaa aagatttgta ttccagattc gaaagcagca attcccccat tgcccgcatc
     7561 acgaaaaaga gcataaaacc aatgatcata taaacgaaaa tgatcgacgg cccggcaagg
     7621 ctaatcgttt tgccggaccc cataaacaac ccagtaccaa tggcacctcc aatggcaata
     7681 agctgaacat gtcggtttgt gagattgcgc cgtagcgact gttcagccgg agcctgatca
     7741 tcggcaacga cttttacctg atctaccatg ttttttcttc ctgtacctgt ctgtgttgtt
     7801 caggctctac ggcctttagt cagtctatga tacgacgaat ctgtatcggg actcatcgat
     7861 attaggtaac aatacgcggg atgaatacta agatttagat ctaatgttaa ttttatgttt
     7921 aaagtgagtg tcatatcacg gtgataacgc gaaacagctc acaaaaatac acgcttacgg
     7981 ggtctttgca acacaaaata gcgccgtttg gcaaactgaa gggtttattg ctgaatgcct
     8041 gctcccctct ggttcgaggg gaacagaatg cgctgcttag aggatttcca gcagttcgac
     8101 ttcaaacacc agggtgctga acggagggat ggatgcacct gcgccgcgct cgccatatgc
     8161 cagttcctgc gggatagtca gttcccattt agaacctacc ggcatcagag tcagtgcttc
     8221 aatccagcca gggatcacgc cattaaccgg gaattcagcg ggttcaccac gagcaacgga
     8281 gctgtcaaac acggtgccgt cgatcagttt accggtgtaa tgaacacgaa cgcggtcggt
     8341 acgtgccgga attgcgcctt caccctggtt gatcacgcgg aattgcaggc cagattcggt
     8401 gctattcaca ccttcttttt tggcgttttc ttccaggtat ttcacgcctt cagcagccat
     8461 cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg tggatttcac gcagcgcgcg
     8521 atgcaccaca tcaaccggca cagccggatg tttgccttcc agcgcatcgg caatacctgc
     8581 aaccagtgct tctggcagca gcccttccag gccagattca ctcagttgtt gccctacctg
     8641 caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca aaagttgggg tggtcatcgt
     8701 atttcctttc ttagggtata aaagtagcgg gcagcatatc agccacactt ctttgggtaa
     8761 aactttgtcg caggaatgat gacaaatccc ggggaaaaag aaacaatagc aatatcccgt
     8821 atgaatataa taaaggcggg tttaccacgg acatcatagc gttagcttat tcataggcta
     8881 tgattgagga acaagacgtg gagcaggagg aaaccatgcc cgggcgcttt gaactaaaac
     8941 caaccctgga gaaagtctgg cacgcgccgg acaattttcg cttcatggac ccgctgccgc
     9001 cgatgcatcg tcgggggatc attattgccg ccattgtatt ggtggtcgga tttctgcttc
     9061 catctgatga tacgcccaac gcgtcggtgg tgacgcgtga agcgcagtta gatattcagt
     9121 cgcaatcaca accgccgaca gaagaacagc cgcaaactac ccaaacgcaa ccattccagc
     9181 cagatagcgg aattgataat caatggcgct cttaccgtgt cgaacccggt aaaacgatgg
     9241 cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt ctatgcaatg gcgcaggtag
     9301 aaggcgcggg taagccgctg agtaatttgc aaaatgggca gatggtgaaa atccgccaga
     9361 acgccagcgg cgtggtgacg ggcttaacca ttgatacggg gaataatcaa caggtcctgt
     9421 ttacccgcca gccggatggc agttttattc gtgcgcggta gatgaagggt caaccaaaag
     9481 ccagctaagg attgtctctt tgatgacctt acgtctggct tccagtgcct ggggggaaac
     9541 catactgata tgataaacta ggccaagcgt atgctggttg atcaaataat atcccccaag
     9601 cgcagcaata ttaatattca cctgcaatgg gtcgatacct ggcttaaaaa tattgtattt
     9661 cttcccttca tccaataagg attccattaa ctgcaagtga gcatggttaa tttctaagag
     9721 acgctgtgat tttgcataat gcacaccttt actttgattt tcactatgca caatttttaa
     9781 aaaccaagga ttagcaatat aataatccca tgtaaaatca ataattttct caatggcttt
     9841 ttgtggcgga agatctgaga aatcaagggc cttttctttt tgtcgaatat cgttccaggt
     9901 atattccagc acttcgataa ataaattttc tttattacca aagtggtgat aaaccgtctg
     9961 tttactacac cctgctgcct ttacaatatt atctactctg gccccttcat aaccatattc
    10021 ggcaaattca ttaaccgcac atagcagcaa tttttccttc aggctctgta cgttgctggt
    10081 atcaatatac ataacgcttc cccacgacct cttcgtggtg actatcataa cctgatttga
    10141 tttcagcttc agaaaaaagg gcaaaaactt gtactttcat caggttatca aatgtcctgt
    10201 gatcgccatc gcatatcaac caactagatg gtttatcttt ttatcctcca gtcgcaagct
    10261 aatcatcacc aaggaaacaa aattcaaaaa agagagctat tgtatgaaat ccactcgtcc
    10321 tgttgccgtg attaccggag ccactcgcgg tattggaaag ggctgtgcgc aggagcttgc
    10381 tcgtggcggg tttaacctgc tgattaacga tcgtcccgat gccgacagcg tagaaaaact
    10441 gcatatcaca caacaagaat gtttggcgga aggcgtggaa gtgatttgct tccctgctga
    10501 tgtcggtgat ctatctctgc atgaggagat gctcgacgcg gcgcaaaacc agtgggggcg
    10561 gctggactgt ctgctcaata acgcggggat ttcagtgaaa aaacgcggtg acctcctcga
    10621 cctcgaacca gacagcttcg atcaaaacat tgccattaac acccgcgcgc cgttcttcct
    10681 tgctcaggca ttcagtaagc gcttactggc acagccgaaa ccggaagcgg agctgccgca
    10741 ccgctcgatt attttcgtca gttccatcaa cgccatcatg ctggcgatga atcgcggcga
    10801 atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt ctgtttgctg ctcgcctttg
    10861 taatgaacaa attggcgttt atgaagtgcg ccctggtctc atcaaaactg atatgactat
    10921 tccggcaacg gcttattacg acgaactgat tgccaaaggt ctggtgccgt ggggacgttg
    10981 gggttatccg gctgatattg cttccaccgt ccgcgcgatg gcggaaggta aactgattta
    11041 cacctgtggt caggcggtcg caatcgacgg cggcttaagt atgccgcgct tctgaggctg
    11101 aatcatgaga gatctgttac aacgcccgga tttattcagt atcaataccg cgacactggg
    11161 ctataaaacg ccgctacccg ccattattga tgcctgcgcc gcacgcggta ttggcgcaat
    11221 tgccccctgg cgcagggagt tgcagggtga agatctccag caaatcaccc gccagcttgc
    11281 cgccagtaac atgagcgttt ccggtttgtg ccgtagcact tactacaccg cgccgacact
    11341 ggcggaacgc aaactggcga ttgatgataa ccgccgggcg ctggacgacg cagcggtgct
    11401 taacgccgcc tgttatatgc aggtggttgg cggtctgcca acgggcacta aagatctata
    11461 tgaagcgcga gaacaagtga aacaagggat tcgccagttg ctgccacatt cgaaagatgt
    11521 cggcgtgcca attgcgctgg agccactgca cccgatgaca gctgccgatc gctcttgcct
    11581 gtgcacgtta cgtcaggcgc tggactggtg tgatgaactc gatcccgatg gtgagtttgg
    11641 cctcggcgtg gcggtggatg tttatcacgt ctggtgggat ccggatctcg ccagtcagat
    11701 cctgcgtgcc ggaaaacgca tcctcgcgtt tcatgtttcc gactggttaa tgccgaccac
    11761 tgatctggtc aatgaccgtg gtatgccggg agatggcgtc atcaatattc cgtcaattcg
    11821 tcggctggtt gaaaacgccg gatttaatgg tgctattgaa ctggaaatat tctcgcctta
    11881 ctggtggcag aaagatatta acag
//
LOCUS       DAZSNM010000124        11895 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZSNM010000124 DAZSNM010000000
VERSION     DAZSNM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11895
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.124"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            263..688
                     /locus_tag="ACHW42_002752"
     CDS             263..688
                     /locus_tag="ACHW42_002752"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR8944503.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            675..1124
                     /locus_tag="ACHW42_002753"
     CDS             675..1124
                     /locus_tag="ACHW42_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR8944504.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            1185..1760
                     /locus_tag="ACHW42_002754"
     CDS             1185..1760
                     /locus_tag="ACHW42_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR8944505.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            1856..2755
                     /gene="yfeX"
                     /locus_tag="ACHW42_002755"
     CDS             1856..2755
                     /gene="yfeX"
                     /locus_tag="ACHW42_002755"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR8944506.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(2844..2930)
                     /locus_tag="ACHW42_002756"
                     /pseudo
     CDS             complement(2844..2930)
                     /locus_tag="ACHW42_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2935..4359)
                     /gene="murP"
                     /locus_tag="ACHW42_002757"
     CDS             complement(2935..4359)
                     /gene="murP"
                     /locus_tag="ACHW42_002757"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFR8944507.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(4363..5259)
                     /gene="murQ"
                     /locus_tag="ACHW42_002758"
     CDS             complement(4363..5259)
                     /gene="murQ"
                     /locus_tag="ACHW42_002758"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR8944508.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            5423..6281
                     /gene="murR"
                     /locus_tag="ACHW42_002759"
                     /pseudo
     CDS             5423..6281
                     /gene="murR"
                     /locus_tag="ACHW42_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
     gene            6410..7201
                     /gene="ucpA"
                     /locus_tag="ACHW42_002760"
     CDS             6410..7201
                     /gene="ucpA"
                     /locus_tag="ACHW42_002760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR8944509.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            7505..8521
                     /gene="cysP"
                     /locus_tag="ACHW42_002761"
     CDS             7505..8521
                     /gene="cysP"
                     /locus_tag="ACHW42_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR8944510.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            8521..9354
                     /gene="cysT"
                     /locus_tag="ACHW42_002762"
     CDS             8521..9354
                     /gene="cysT"
                     /locus_tag="ACHW42_002762"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR8944511.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            9354..10229
                     /gene="cysW"
                     /locus_tag="ACHW42_002763"
     CDS             9354..10229
                     /gene="cysW"
                     /locus_tag="ACHW42_002763"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR8944512.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            10219..11316
                     /gene="cysA"
                     /locus_tag="ACHW42_002764"
     CDS             10219..11316
                     /gene="cysA"
                     /locus_tag="ACHW42_002764"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR8944513.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            11450..>11895
                     /locus_tag="ACHW42_002765"
     CDS             11450..>11895
                     /locus_tag="ACHW42_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HFR8944514.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYA"
BASE COUNT         2753 a         2983 c         3379 g         2780 t
ORIGIN      
        1 gcacctggcg gcgcgaagtg agtgttttta gtggtttaaa agtgctcata cggcctgagt
       61 tgtaataata aagttccaga tgttatatcg tatcgcgtaa tccgttacga cggtttgctg
      121 ttgagaattg ttttactttt catttcaata gttgacaact ccccattatg ccactttttc
      181 aaccgatttt cgtcagatat tggctaattc gaccgttcac gccataatca gccttttaac
      241 ccgcaaaaag gtaacgaata ccatggagat acgcgtattt cgccaggaag atttcgaaga
      301 ggtcatcacc ctttgggagc gttgcgactt gctgcgtccg tggaacgatc cggaaatgga
      361 catcgagcgt aagatgaacc atgacgtcag tttgtttttg gtcgctgagg taaacggtga
      421 agtggtcgga acggtgatgg gcggttatga cgggcatcgc gggtctgctt attatcttgg
      481 cgtgcatcca gagtttcgtg ggcgtggaat tgccaatgcg ttgcttaacc ggctggagaa
      541 aaagctgatt gctcgcggct gcccgaaaat tcagatcaac gtgccggaag ataacgacat
      601 ggtgctcgga atgtatgaac gcctgggata tgaacacgcc gacgtgctga gtctgggtaa
      661 gcgtttgatt gaagatgaag agtactgagt tccatcctgt ccattatgat gcgcacggac
      721 gcctgcgttt acccttgctc ttctggcttg tgctattgct tcaggcgcga acctgggtgc
      781 tgtttgtcat tgccggtgcg tcgcgtgagc agggtactgc gctgctaaat ctgttttatc
      841 ccgatcacga taatttctgg ctggggctga ttcccggtat tccggcggtg ctggcgtttt
      901 tgttgagcgg tcggcgggct tcgtttcctc gcacctggca tgtgctctat tttctgttgc
      961 tgttggcgca ggtggtttta ctttgctggc aaccgtggct gtggctgaac ggtgaatccg
     1021 ttagcggtat cggtctggcg ctggtagtgg cggatattgt ggcgttaatc tggctgctga
     1081 ccaatcgacg tttacgcgct tgttttaatg aagaaaaaga ataacggcac tttttggtga
     1141 atttgcactc caagcaacgt tattgaataa ccaaaggaag tgacatgaaa tcgctgcgtt
     1201 taatgttatg cgctatgccg ttgatgctga ccggctgttc cacgatgtcg tcagttaact
     1261 ggtctgccgc taacccgtgg aactggtttg gatcgtccac caaagtgagc gagcagggcg
     1321 tgggtgaatt aacggcgtcc acaccactgc aagaacaagc cattgctgat gcgcttgatg
     1381 gcgattatcg cctgcgcagc ggaatgaaaa ccacgaacgg caacgtggtg cgcttttttg
     1441 aagtgatgaa aggcgacaac gtggcgatgg tgattaacgg cgatcaaggc acgatcagtc
     1501 gcattgacgt gctggatagc gatattcctg ctgacaccgg tgttaaaatc ggtacaccgt
     1561 ttagcgacct ttacagcaaa gcatttggca attgccaaaa agctgatggt gatgataatc
     1621 gtgccgtcga atgtaaagcc gaaggcagtc aacatattag ctaccagttc agcggggaat
     1681 ggcgtggtcc tgaagggtta atgccttcgg acgataccct gaaaaactgg aaagtcagta
     1741 aaattatctg gcggcgttaa tttgcgtctg aacaaaccgc cgtcgcgaaa aaacacgtaa
     1801 aatagcgctc aacaatgcca cggattgcgt ggcattctta ttttcaggag gaacaatgtc
     1861 tcaggttcag agtggcattt tgccagaaca ttgccgcgcg gcgatttgga tcgaagccaa
     1921 cgtgaaaggg gaagttgacg ccctgcgtgc ggccagtaaa acatttgccg acaaactggc
     1981 aacttttgaa gcgaaattcc cggacgcgca tcttggtgcg gtggttgcct ttggtaacaa
     2041 cacctggcgc gctctgagcg gcggcgttgg ggcagaagag ctgaaagatt ttccgggcta
     2101 cggtaaaggc cttgcgccga cgacccagtt cgatgtgttg atccacattc tttctctgcg
     2161 tcacgacgta aacttctctg tcgctcaggc ggcgatggaa gcctttggtg actgcattga
     2221 agtgaaagaa gagatccacg gcttccgttg ggtggaagag cgtgacctga gcggctttgt
     2281 tgacggtaca gaaaacccgg cgggtgaaga gacgcgccgc gaagtggcgg ttatcaaaga
     2341 cggcgtggat tccggcggca gctatgtgtt tgtgcagcgt tgggagcaca acctgaagca
     2401 gctcaaccgg atgagcgttc acgatcagga gatgatgatc gggcgcacca aagaggccaa
     2461 cgaagagatt gacggcgacg aacgtccgga aacctctcac ctcacccgcg ttgatctgaa
     2521 agaagatggc aaagggctga agattgttcg ccagagcctg ccgtacggca ccgccagtgg
     2581 cactcacggt ctgtacttct gcgcctactg cgcgcgtctg cataacattg agcagcaact
     2641 gctgagcatg tttggcgata ccgatggcaa acgtgatgcg atgttgcgtt tcaccaaacc
     2701 ggtaaccggc ggctattatt tcgcaccgtc gctggacaag ttgatggcgc tgtaagcgtt
     2761 tctgattcat tgattaaagg ccaggcagta acattactga ctggcctttt tattactgct
     2821 ttaacgccgc atatacttga tcaactacct tctgtcagaa cagggtattt tgcggcgctg
     2881 acgctacaac taacagccag cagagaaaga tagagcattg tccgtttcat aatatcagtc
     2941 cagattgacg ttacggcagc caaagagcgt ggtgaaaata aacccgcaaa cccatgccac
     3001 cagaatcccg ccagcataaa ccgccattgc cggaaggatg ccttgtgcgg aagtcatcag
     3061 cggcagcgcc accagaccag acggcccaaa ggcgctgttt aagcccatcg gcagacccca
     3121 ccaggctatc aatccgataa acaaaccgcc cgccgcgccg cctaaacagg cggtaacaaa
     3181 cggcttcatg cggggcaggg tgacaccgta aatcagcggt tcaccaacgc ccagcaggcc
     3241 gggaataatc gccccgcgta cctgactgcg tagcgcactg tgcggttgcg cccgccagta
     3301 gagtgccagc gccgcgccca cctggcccgc gcctgccatt gaaaggatgg gaaataagct
     3361 gttgaatccc tggctgtcca ttaacgcgag gtaaacagga ataaagccct gatgcacgcc
     3421 aaataccacg gcgatcagga acagcccggc taaaaccgca caaccgaacg gattactgtt
     3481 caggtgcata aacagccatg acataccttc gaataaccag ccacccagcg gcataatgat
     3541 caggtacgca agcgtagcgg tgatcagcag ggtgattaac gaggtcagca gcatgtcgag
     3601 atcgtccggc ataaagcggc gcaccatgcc ttcaatgcgg gcgcaggccc acgcggcaat
     3661 cagcacgccg ataatattgc cgcgcggatc gatgggcaga ccaaagaaat cgtgaaaacc
     3721 ggcgtagtaa ccggtggttg cagcggggtt ataaccgagc aaaaagagtg cggcaataat
     3781 tgcgccattg acgcccgtgc cgccaaatgc ctgggcggcg ttatagccca ccagaatcac
     3841 taggaaggtg aacaaacctt tgctgaacac cttcataaaa ttcagcgcgt cgggtagcgt
     3901 gccctgagca tctgccggaa cgtgcatcac cgtggcaatt aacgtcgcta tccccagcag
     3961 cagaccggcg gcaataaaac cggggatcag cggcgtgaag atggtggcga atttggcgag
     4021 aaattgttgc acgccggagg tttgtttggc ttttaactga cgtttgttct gggcggcgat
     4081 ttctgcggca tcctgtaccg gtgcatcgcc cagcagctcg ctcatcgctt ttgcggcacg
     4141 atgcgcttta ccaggcccaa aaacgacctg cacctggtcg ctggtcaaaa tgacgccctt
     4201 cacgccttcc agcgttttga tattgggatc aaccagtgaa ctgtcatgta cacccagacg
     4261 caggcgcgtc atacagttac cacaactggc gatatttccc ggtccgccga cacgggtaag
     4321 aatggtgttc agaagttcac tgctgatctc tttggccatg ggttattcct tgtctaaaac
     4381 ctgacgaata aagccgccgt gttgatccag gcgtttttta gcttctgcgg catcgagatt
     4441 tttcagcacc atcacaatgg ccgttttaca gttgcgctcg caggcaatta acgccgcttc
     4501 cgcttgttcg gcattacatc cggtggcgtt tttaacgata ttgacctggc gcacatgcag
     4561 tttttcgttg gtggcgacca catcgaccat caggttgccg aacactttgc cggatttaat
     4621 catcagcccg gtggaaagca tattgagcac cagtttctgc gctgtacctg ctttcatccg
     4681 cgaagaaccg gtaacaactt cggcacctac aatcggtgta atggcaaact cagcggtggt
     4741 tgaaacggcg ctccccggat tacaggaaat tcccactgtg cggcagccga gctggcgtgc
     4801 gtattccagt ccggcaatca catacggcgt gcgaccgctg gcagcaatgc caaccaccac
     4861 atcctgtgcc gttaaattaa tatttttcag atcgttaaca ccaccttccc ggctatcttc
     4921 agctccttcc accgcgtgct gaatggcata ttcgcctcca gcaatcaaac caaccaccag
     4981 acccggtttc acgccgtagg tgggcggaca ttcgctggca tccagaatcc ccagacgacc
     5041 ggatgttccc gcaccgagat aaatcagacg accgccgccg ctgacctggg cgtggataac
     5101 atcgatcgcc gcggcgatat ccggcaggac gcgatcaacg gcaggcggta cggttttatc
     5161 ttcatcgttg ataatccggc acatttccag cgtcgatacg cggtcaattt cagccgaggc
     5221 ggtgttcgag ccttcagtaa tcatcttttc tagttgcatc ggtggtgacc ttactattta
     5281 cgatcaaata attgcagatg tatggaatca tatatcccga attgacagac ttaaaggaat
     5341 cattgagtct acactcgcag agtgtgaaga gcgtcataag aacttaagcg gaatttttgc
     5401 tgccagccca ggagagtgga agatgttgta cctgacgaaa atacgcaatg cggaaagtga
     5461 atttaccggg aacgagcaaa aaattgctga ctttctgcgg gccagagtga gcgagctgaa
     5521 atccgtctct tcacggcaga tggcgaaaca gctcggtatc agccagtcga gcattgtgaa
     5581 atttgcccag aagctcggcg cgcaaggatt tactgaattg cgtatggcgt taattggcga
     5641 atacagtgcc agccgggaga aaacgaatgc cacggcgctg cacctgcaca gttcgattac
     5701 cagtgatgat tcgctggaag tgattgcccg caaactgaat cgcgaaaaag agctggcgct
     5761 ggagcaaacc tgtgcgttgt tcgactacgc gcggctgcaa aaaatcattg aggtcatcag
     5821 taaagcaccg tttatccaga taacgggcct gggcggatcg gcgctggtgg ggcgcgatct
     5881 gtcgttcaaa ctgatgaaaa ttggctatcg cgtggcctgc gaagccgaca cgcacgtaca
     5941 agccaccgtt tcccaggcgt tgaaaaaagg cgatgtacaa atcgctatct cttacagcgg
     6001 cagtaaaaaa gagattgtct tgtgcgcaga agcggcacga aagcaggggg caacggtgat
     6061 tgccattact tctctggctg actctccgct gcggcggctg gcgcatttca cgctcgatac
     6121 cgtttccggt gaaaccgaat ggcgtagctc gtcgatgtct acccgcacgg cgcaaaattc
     6181 tgttaccgat ttactgtttg ttggtcttgt gcaacatcaa tgatgtggcg tcattgaaaa
     6241 tgattcagcg cagcagtgag ctaacacaac gcctgaaatg atgcataaag cagcgactgg
     6301 attgagattt tcctgaatta gtgagctgat ccgcagcaat attttgttta tcctgtattt
     6361 tcagagggaa tggagtgtaa cgctctgtat taacaaggag agcattaaaa tgggtaaact
     6421 cacgggcaag acagcactga ttacgggcgc attgcaggga attggcgaag gaattgccag
     6481 aacttttgca cgtcatggcg cgaacctaat cttgctggat atctcccctg agatcgaaaa
     6541 gctagcggac gaactgtgtg gtcgtggtca tcgctgtacg gcggttgtcg ccgatgtgcg
     6601 tgacccggcg tcggtagccg cagctatcaa acgcgcgaag gaaaaagaag ggcgcattga
     6661 tatcctggtg aataacgcag gcgtttgtcg tctgggcagt ttcctcgata tgagcgatga
     6721 agatcgcgat ttccatattg atatcaatat taaaggcgta tggaacgtca cgaaggcggt
     6781 gctgccggag atgattgcgc gcaaagatgg tcgcattgtg atgatgtctt cagtcactgg
     6841 tgatatggtg gccgatcctg gcgaaacggc gtatgcctta acgaaagcgg cgattgttgg
     6901 cctgactaaa tcgctggcgg tggagtacgc gcaatccggt attcgcgtta acgccatctg
     6961 cccgggatac gtccgcacgc caatggcgga aagcattgcc cgccagtcga acccggaaga
     7021 tccagaatcg gtgctgactg aaatggcaaa agcaatcccg ctgtgtcgcc tcgccgatcc
     7081 gctggaagtc ggcgaactgg cggccttcct cgcatcggat gaatccagct atttaaccgg
     7141 tacacagaat gtgattgatg gcggcagcac actgccggag acggttagcg tcggtatctg
     7201 attcacctct gtttcctccc tgcatgtgtg gggaggattt cgtcttgaac taagttcacc
     7261 aggctatttc atttgtcatt ttggccccgg gcagtgctcg aaatcctcac gtactatgtg
     7321 tacgctccgg tttctccgcg ctgtccgtgt ccaaactgcc tgcaacaatt acgcctgttg
     7381 aaccaagttc ttattccctt tttaacttcc aaatcaccaa acggtatata aaaccgttac
     7441 tcctttcacg tccgttataa atatgatggc tattagaaag tcattaaatt tataagggtg
     7501 cgcaatggcc gttaacttac tgaaaaagaa ctcactcgcg ctggtcgctt ctctgctgct
     7561 ggcgggccat gtacaggcaa cggaactgct gaacagttct tatgacgtct cccgcgagct
     7621 gtttgccgcc ctgaatccgc cgtttgaaca gcaatgggca aaagataacg gcggcgacaa
     7681 actgacgata aaacaatctc atgccgggtc atcaaaacag gcgctggcga ttttgcaggg
     7741 cttaaaagca gacgttgtca cttataacca ggtgaccgac gtacaaatcc tgcacgacaa
     7801 aggcaagctg atcccggctg actggcagtc gcgcctgccg aataatagct cgccgttcta
     7861 ctccaccatg ggcttcctgg tgcgtaaggg caacccgaag aatatccacg actggaacga
     7921 cctggtgcgc tccgacgtga agctgatttt cccgaacccg aaaacgtcgg gtaacgcgcg
     7981 ttatacctat ctggcggcat ggggcgcagc ggataaagct gacggtggcg ataaagccaa
     8041 aaccgaacag tttatgaccc agttcctgaa aaacgttgaa gtgttcgata ccggcggtcg
     8101 tggcgcaacc accacttttg ccgagcgtgg cttgggcgat gtgctgatta gctttgaatc
     8161 ggaagtgaac aacatccgta aacagtatga agcacagggc tttgaagtgg tgatcccgaa
     8221 aaccaatatt ctggcggaat tcccggtggc gtgggtcgat aaaaatgtgc aggccaacgg
     8281 tacggaaaaa gccgcaaaag cctacctgaa ctggctctac agcccacagg cgcagaccat
     8341 catcaccgac tattactacc gtgtgaataa cccggaagtc atggacaaac tgaaagataa
     8401 attcccgcag accgaactgt tccgcgtgga agacaaattt ggctcatggc cggaagtgat
     8461 gaaaacccac ttcaccagcg gcggcgagtt agacaagctg ttagcggcgg ggcgtaagta
     8521 atgtttgcag tttcttcaag acgcgtgctg ccgggcttta ctttaagcct tggcaccagt
     8581 ctgctgtttg tgtgcctgat attgctgctg ccgctctctg cgctggtgat gcagctttct
     8641 gagatgagct gggcgcagta ctgggaggtg atcaccaacc cgcaggtggt cgcggcctac
     8701 aaagtgacgc tgctgtcggc gtttgtggca tcgattttta acggcgtttt cggtctgctg
     8761 atggcgtgga ttttaacacg ttatcgcttt ccgggccgca cgctgcttga tgcgctgatg
     8821 gatttaccct ttgcgctgcc aacggcagtc gccggtttaa cgctggcctc gctcttttcc
     8881 gtaaacggtt tttacggtga atggctggcg aagtttgata tcaaagtcac ctatacctgg
     8941 cttggtattg cggtggcgat ggcctttacc agcattccgt ttgtggtgcg taccgtgcag
     9001 ccggtgctgg aagagttagg cccggaatat gaagaagcgg cggaaacgct tggtgcaacg
     9061 cgctggcaga gtttctgcaa agtggtgctg ccggagcttt ctccggcgct ggtggcgggc
     9121 gtggcgctgt cgtttacccg tagtcttggt gaatttggcg cggtgatttt tatcgccgga
     9181 aatatcgcgt ggaagacgga agtgacgtcg ctgatgattt ttgtgcgctt acaggagttt
     9241 gattacccgg cagcgagcgc gattgcttcg gtgatcctcg cggcatctct gctgctgctg
     9301 ttctcaatta acactctgca aagtcgcttt ggtcggcgtg tggtaggtca ttaatggcgg
     9361 aagttaccca attgaagcgt tatgacgcgc gcccgattaa ctggggcaaa tggtttctga
     9421 ttggcatcgg gatgctggtt tcggcgttca tcctgctggt gccgatgatt tacatcttcg
     9481 tgcaggcatt cagcaagggg ctgatgccgg ttttacagaa tctggccgat ccggacatgc
     9541 tgcacgccat ctggctgacg gtgatgatcg cgctgattgc cgtaccggta aacctggtgt
     9601 tcggcattct gctggcctgg ctggtgacgc gctttaactt cccgggacgc cagttactgc
     9661 tgacgctact ggacattccg tttgctgtat cgccggtggt tgccggtctg gtgtatctgc
     9721 tgttctacgg ctccaacggc ccgctcggcg gctggctgga tgaacataat ctgcaaatta
     9781 tgttctcctg gcccggaatg gtgctggtca ccatctttgt gacgtgtccg tttgtggtgc
     9841 gcgagctggt gccggtgatg ttaagccagg gcagccagga agacgaagcg gcgattttgc
     9901 ttggcgcgtc cggctggcag atgttccgtc gcgtcacatt gccgaatatt cgctgggcgc
     9961 tgctttacgg cgtggtgctg accaacgccc gcgcgattgg cgagtttggt gcagtgtcgg
    10021 tggtttccgg ctcgattcgc ggcgaaacac tttcgctgcc gttacagatt gaattgttgg
    10081 agcaggacta caacaccgtc ggctccttta ccgccgccgc gctgttgacg ttgatggcga
    10141 ttatcaccct gtttttaaag agtatgttgc agtggcgcct ggagaatcag gaaaaacgcg
    10201 cgcagcagga ggagcatcat gagcattgag attgccaata tcaagaagtc ttttggtcgc
    10261 acccaggtgc tgaacgatat ctcactggat attccttctg gtcagatggt cgcgttgctg
    10321 gggccgtccg gttccgggaa aaccacgctg ctgcggatta tcgccgggct ggagcatcaa
    10381 accagcgggc atattcgctt ccacggcacc gacgtgagtc gcctgcacgc gcgcgatcgt
    10441 aaagtcggtt tcgtgttcca gcattacgcg ctgttccgcc atatgacggt attcgacaat
    10501 atcacttttg gcctgacggt gctgccgcgt cgcgagcgcc cgaatgccgc agccatcaaa
    10561 gcgaaagtga caaaattgct ggaaatggtc cagcttgcgc atctggcgga tcgttatccg
    10621 gcgcagcttt ccggcggcca gaaacagcgt gtggcgctgg cgcgcgcgct ggctgtagaa
    10681 ccgcaaattc tgctgcttga tgaaccgttc ggcgcgctgg atgcgcaggt gcgtaaagag
    10741 ctgcgtcgct ggctgcgtca actgcatgaa gagctgaaat tcaccagcgt gtttgtgacc
    10801 cacgaccagg aagaggcgac cgaagtagcc gatcgtgtag tggtgatgag tcaggggaat
    10861 attgaacagg ctgatgcgcc gaatcaggta tggcgcgaac cagcgacccg ttttgtactc
    10921 gaatttatgg gcgaagtgaa ccgcctgcag ggaaccattc gcggcgggca gttccatgtt
    10981 ggcgcgcatc gctggccgct gggctacaca cctgcgtatc aggggccggt ggatctcttc
    11041 ctgcgcccgt gggaagtgga tatcagccgc cgtaccagtc tcgattcgcc gctgccggta
    11101 caggtactgg aagccagccc gaaaggtcac tacacccaat tagtggtgca gccgctgggg
    11161 tggtacaacg aaccgctgac ggtcgtgatg catggcgacg atgccccgca gcatggcgag
    11221 cgtttattcg ttggtctgca acatgcgcgg ctgtataacg gcgacgagcg tattgaaacc
    11281 cgcgatgagg aacttgctct cgcacaaagc gcctgatagg ttgagtgaat gttaaacgcc
    11341 cggaggcgct tcccgcgatc cgggcttttt aatggcaagg tttgtaacct gtaggcctga
    11401 taagacgcgc aagcgtcgca tcaggcaaca ccacgtatgg atagagattg tgagtacatt
    11461 agaacaaaca ataggcaata cgcctctggt gaagttgcag cgaatggggc cggataacgg
    11521 cagtgaagtg tggttaaaac tggaaggcaa taacccggca ggttcggtga aagatcgtgc
    11581 ggcactttcg atgatcgtcg aggcggaaaa gcgcggggaa attaaaccgg gtgatgtctt
    11641 aatcgaagcc accagtggta acaccggcat tgcgctggca atgattgccg cgctgaaagg
    11701 ctatcgcatg aaattgctga tgcccgacaa catgagccag gaacgccgtg cggcgatgcg
    11761 tgcttatggt gcggaactga ttcttgtcac caaagagcag ggcatggaag gtgcgcgcga
    11821 tctggcgctg gagatggcga atcgtggcga aggaaagctg ctcgatcagt tcaataatcc
    11881 cgataaccct tatgc
//
LOCUS       DAZSNM010000125        11656 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZSNM010000125 DAZSNM010000000
VERSION     DAZSNM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11656
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.125"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..309
                     /locus_tag="ACHW42_002766"
     CDS             <1..309
                     /locus_tag="ACHW42_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR8944515.1"
                     /translation="RFINTDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDH
                     GKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM
                     "
     gene            complement(508..858)
                     /locus_tag="ACHW42_002767"
                     /pseudo
     CDS             complement(508..858)
                     /locus_tag="ACHW42_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(1061..2074)
                     /gene="intI1"
                     /locus_tag="ACHW42_002768"
     CDS             complement(1061..2074)
                     /gene="intI1"
                     /locus_tag="ACHW42_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR8944516.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2232..2705
                     /gene="dfrA17"
                     /locus_tag="ACHW42_002769"
     CDS             2232..2705
                     /gene="dfrA17"
                     /locus_tag="ACHW42_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR8944517.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            2836..3624
                     /locus_tag="ACHW42_002770"
     CDS             2836..3624
                     /locus_tag="ACHW42_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFR8944518.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            3830..4177
                     /locus_tag="ACHW42_002771"
     CDS             3830..4177
                     /locus_tag="ACHW42_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR8944519.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            4171..5010
                     /gene="sul1"
                     /locus_tag="ACHW42_002772"
     CDS             4171..5010
                     /gene="sul1"
                     /locus_tag="ACHW42_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR8944520.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            5138..5341
                     /locus_tag="ACHW42_002773"
     CDS             5138..5341
                     /locus_tag="ACHW42_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944521.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            5497..6702
                     /gene="chrA"
                     /locus_tag="ACHW42_002774"
     CDS             5497..6702
                     /gene="chrA"
                     /locus_tag="ACHW42_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFR8944522.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            6713..7018
                     /locus_tag="ACHW42_002775"
     CDS             6713..7018
                     /locus_tag="ACHW42_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR8944523.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            7245..8009
                     /locus_tag="ACHW42_002776"
     CDS             7245..8009
                     /locus_tag="ACHW42_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFR8944524.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(8502..9086)
                     /gene="mphR(A)"
                     /locus_tag="ACHW42_002777"
     CDS             complement(8502..9086)
                     /gene="mphR(A)"
                     /locus_tag="ACHW42_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFR8944525.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(9086..10324)
                     /gene="mrx(A)"
                     /locus_tag="ACHW42_002778"
                     /gene_synonym="mrx"
     CDS             complement(9086..10324)
                     /gene="mrx(A)"
                     /locus_tag="ACHW42_002778"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFR8944526.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(10321..11226)
                     /locus_tag="ACHW42_002779"
     CDS             complement(10321..11226)
                     /locus_tag="ACHW42_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR8944527.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            complement(11348..>11656)
                     /locus_tag="ACHW42_002780"
     CDS             complement(11348..>11656)
                     /locus_tag="ACHW42_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR8944528.1"
                     /translation="RFINTDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDH
                     GKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM
                     "
BASE COUNT         2451 a         3350 c         3409 g         2446 t
ORIGIN      
        1 cgattcatca acacggataa agcgcccgcc tatggtcgcg cgcttgctct gctcaaacgc
       61 gaaggccggt gcccgtctga cgttgaacac cgacagatta agtaccggaa caacgtgatt
      121 gaatgcgatc atggcaaact gaaacggata atcaacgcca cgctgggatt taaatccatg
      181 aagacggctt acgccaccat caaaggtatt gaggtgatgc gtgcactacg caaaggccag
      241 gcctcagcat tttattatgg tgatcccctg ggcgaaatgc gcctggtaag cagagttttt
      301 gaaatgtaag gcctttgaat aagacaaaag gctgcctcat cgctaacttt gcaacagtgc
      361 ccccaatgcg ctgtccagtc atggaattcc ctgcaaaatg tcagggaaga ctctatgacc
      421 ttcaacgaga tatgtcaata aattcaaaat tcaatcctat cctgacgcaa tttacacatg
      481 gcatctgaca tcaggttagg gtatgcctca acctgacggc ggcgaatcac aagcgtccgg
      541 tttgacgctg gtttcggtcg cagtgctggc ccgcgcctgg aagcgctgat agcactccag
      601 cccgcagaaa tgctccacgt actcggcccc ttccggcgtg aaggcggcat cgagcgggat
      661 ttccttgcag cacacgcagc aactggtgct cggttcattg gcgttcatgg tggtgttcct
      721 ccattggttg acgaagccga cgaaggccgc cgccggcatc ggcctggcga acaggaaacc
      781 ctgcaccgtg tcgcaacccg cctgccgcaa ccacgcaagg cagtcgggtg tttccacacc
      841 ctcggctacc acctccattg tcgttttcag aagacggctg cactgaacgt cagaagccga
      901 ctgcactata gcagcggagg ggttggatcc atcaggcaac gacgggctgc tgccggccat
      961 cagcggacgc agggaggact ttccgcaacc ggccgttcga tgcggcaccg atggccttcg
     1021 cgcaggggta gtgaatccgc caggattgac ttgcgctgcc ctacctctca ctagtgaggg
     1081 gcggcagcgc atcaagcggt gagcgcactc cggcaccgcc aactttcagc acatgcgtgt
     1141 aaatcatcgt cgtagagacg tcggaatggc cgagcagatc ctgcacggtt cgaatgtcgt
     1201 aaccgctgcg gagcaaggcc gtcgcgaacg agtggcggag ggtgtgcggt gtggcgggct
     1261 tcgtgatgcc tgcttgttct acggcacgtt tgaaggcgcg ctgaaaggtc tggtcataca
     1321 tgtgatggcg acgcacgaca ccgctccgtg gatcggtcga atgcgtgtgc tgcgcaaaaa
     1381 cccagaacca cggccaggaa tgcccggcgc gcggatactt ccgctcaagg gcgtcgggaa
     1441 gcgcaacgcc gctgcggccc tcggcctggt ccttcagcca ccatgcccgt gcacgcgaca
     1501 gctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc
     1561 ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt
     1621 gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct
     1681 cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc
     1741 gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct
     1801 tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc
     1861 gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt
     1921 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     1981 gtaaactgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     2041 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgtac
     2101 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     2161 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttagccatta
     2221 agggagttaa attgaaaata tcattgattt ctgcagtgtc agaaaatggc gtaatcggta
     2281 gtggtcctga tatcccgtgg tcagtaaaag gtgagcaact actctttaaa gcgctcacat
     2341 ataatcaatg gctccttgtc ggaagaaaaa catttgactc tatgggtgtt cttccaaatc
     2401 gcaaatatgc agtagtgtca aagaacggaa tttcaagctc aaatgaaaac gtcctagttt
     2461 ttccttcaat agaaaatgct ttgaaagagc tatcaaaagt tacagatcat gtatatgtct
     2521 ctggcggggg tcaaatctat aatagcctta ttgaaaaagc agatataatt catttgtcta
     2581 ctgttcacgt tgaagtcgaa ggtgatatca aattccctat aatgcctgag aatttcaatt
     2641 tggtttttga acagtttttt atgtctaata taaattatac ataccagatt tggaaaaaag
     2701 gctaacaatg cgttgcagca ccagtcgctt cgctccttgg acagctttta agtcgcgtct
     2761 ttgtggtttt gctgcgcaaa agtattccac aaagccgcaa cttaaaagct gccgctgaac
     2821 ttaacgttag gcatcatggg tgaatttttc cctgcacaag ttttcaagca gctgtcccac
     2881 gctcgcgcgg tgatcgagcg ccatctggct gcgacactgg acacaatcca cctgttcgga
     2941 tctgcgatcg atggagggct gaagccggac agcgacatag acttgctcgt gaccgtcagc
     3001 gccgcaccta acgattcgct ccggcaggcg ctaatgctcg atttgctgaa agtctcatca
     3061 ccgccaggcg atggcggaac atggcgaccg ctggagctaa ctgttgtcgc tcgaagcgaa
     3121 gtagtgcctt ggcgctatcc ggcgcggcgt gagcttcagt tcggtgagtg gctccgccac
     3181 gacatccttt ccggaacgtt cgagcctgcc gttctggatc acgatcttgc gattttgctg
     3241 accaaggcga ggcaacacag ccttgcgctt ctaggcccat ccgcagccac gtttttcgag
     3301 ccggtgccga aggagcattt ctccaaggcg cttttcgaca ctattgccca gtggaatgca
     3361 gagtcggatt ggaagggtga cgagcggaac gtcgttcttg ctcttgctcg catttggtac
     3421 agcgcttcaa ctggtctcat tgctcctaag gacgttgctg ccgcatgggt atcggagcgt
     3481 ttgcctgccg agcatcggcc cctcatctgc aaggcacgcg cggcgtacct gggtagcgag
     3541 gacgacgacc tagcaatgcg cgtcgaagag acggccgcgt tcgttcgata tgccaaagca
     3601 acgattgaga gaatcttgcg ttgagcggca tgtgcgaaaa gtgcatcgac ccgcgccgag
     3661 ggcatctgat gcctaactcg gcgttcaagc ggacgggctg cgcccgccgc tcaactatgc
     3721 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     3781 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgaaaggctg
     3841 gctttttctt gttatcgcaa tagttggcga agtaatcgca acatccgcat taaaatctag
     3901 cgagggcttt actaagcttg ccccttccgc cgttgtcata atcggttatg gcatcgcatt
     3961 ttattttctt tctctggttc tgaaatccat ccctgtcggt gttgcttatg cagtctggtc
     4021 gggactcggc gtcgtcataa ttacagccat tgcctggttg cttcatgggc aaaagcttga
     4081 tgcgtggggc tttgtaggta tggggctcat aattgctgcc tttttgctcg cccgatcccc
     4141 atcgtggaag tcgctgcgga ggccgacgcc atggtgacgg tgttcggcat tctgaatctc
     4201 accgaggact ccttcttcga tgagagccgg cggctagacc ccgccggcgc tgtcaccgcg
     4261 gcgatcgaaa tgctgcgagt cggatcagac gtcgtggatg tcggaccggc cgccagccat
     4321 ccggacgcga ggcctgtatc gccggccgat gagatcagac gtattgcgcc gctcttagac
     4381 gccctgtccg atcagatgca ccgtgtttca atcgacagct tccaaccgga aacccagcgc
     4441 tatgcgctca agcgcggcgt gggctacctg aacgatatcc aaggatttcc tgaccctgcg
     4501 ctctatcccg atattgctga ggcggactgc aggctggtgg ttatgcactc agcgcagcgg
     4561 gatggcatcg ccacccgcac cggtcacctt cgacccgaag acgcgctcga cgagattgtg
     4621 cggttcttcg aggcgcgggt ttccgccttg cgacggagcg gggtcgctgc cgaccggctc
     4681 atcctcgatc cggggatggg atttttcttg agccccgcac cggaaacatc gctgcacgtg
     4741 ctgtcgaacc ttcaaaagct gaagtcggcg ttggggcttc cgctattggt ctcggtgtcg
     4801 cggaaatcct tcttgggcgc caccgttggc cttcctgtaa aggatctggg tccagcgagc
     4861 cttgcggcgg aacttcacgc gatcggcaat ggcgctgact acgtccgcac ccacgcgcct
     4921 ggagatctgc gaagcgcaat caccttctcg gaaaccctcg cgaaatttcg cagtcgcgac
     4981 gccagagacc gagggttaga tcatgcctag cattcacctt ccggccgccc gctagcggac
     5041 cctggtcagg ttccgcgaag gtgggcgcag acatgctggg ctcgtcagga tcaaactgca
     5101 ctatgaggcg gcggttcata ccgcgccagg ggagcgaatg gacagcgagg agcctccgaa
     5161 cgttcgggtc gcctgctcgg gtgatatcga cgaggttgtg cggctgatgc acgacgctgc
     5221 ggcgtggatg tccgccaagg gaacgcccgc ctgggacgtc gcgcggatcg accggacatt
     5281 cgcggagacc ttcgtcctga gatccgagct cctagggatc gcctcagaaa acggaaaata
     5341 aagcacgcta agccgtaagt aagcgtgctc ctgtgaaagc cacagctaaa actgcgtagt
     5401 acacatagag gtattttttt ctagatttat atcgaatttc gttatcgaat ttcgcttatt
     5461 attgatcaac tcgttgcaga tcatgaaggc gaaagtatga cgaataaccc taccgatgac
     5521 agcagaccat ggtcggtctt tcttattttt ctgcggcttg gattgacatc ttttggcggc
     5581 cccattgcgc acttgggcta cttccgcgcc gaatttgtca cacggcggcg ctggctctcc
     5641 gaacggagct atgctgactt ggtcgcgctt tgtcagttct tgccagggcc tgcaagcagc
     5701 caggtcggca tagcggtagg actgtctcgg gctggataca gcggggcgct ggctgcttgg
     5761 gctggcttca cgctgccgtc tgccatagcc ttgatccttt ttgcgctcgg catctccagc
     5821 tatggcgatt acgtctcgca gggcgcgttg catggcttaa aagtggtggc tgtggccgtg
     5881 gtcgctcaag cagtatgggg catggcgcgt aacctatgca cggatgggct gcgagtcacc
     5941 atcatggcaa ttgctacctg cgtcgtttta cttgtgccgt ccgcgtgggg acaggttggc
     6001 gtgattgcta tcgcaggcat cgcaggccgg ttattgttca agccagcgaa agttgttgag
     6061 catgaccccc tacctatcac ggtcagtcac cgggccggcg tgctttggct ctcgctgttc
     6121 tttgtcttgc tgattggcct gccggtgttg gccgaactga tgccaagtca aaccatggca
     6181 atggtggatt ccttctatcg tgtcggatca ctggtgttcg gcggtggtca cgttgtgctg
     6241 ccattactgc aagccgaagt ggtgccctcc ggctgggtca acaatgaatc ctttctcgcg
     6301 gggtacgggg cagctcaagc ggtgcccggc cctttgttca cgttcgccgc gtttcttggt
     6361 gcctcgatga acaccgcccc gtcgggctgg atcggcggca ttgtgtgtct gctggctatc
     6421 ttcgcgccct cgttcttgct ggtcgtcgga tcaatgccat tttgggagcg tttgcgccgc
     6481 aatacaggca tccaagctgc gctggccggg atcaatgccg ctgtagtcgg cttgctgctg
     6541 gccgcgctgt atcagcctgt atggactagc gccatctttc agccgcaaga cttcggcttg
     6601 gcattagttg cccttgtcgc acttatgttc tggaagctcc cgccgtggct ggttgtcctc
     6661 ggtagcggtg cagctgggtg gctgttgagc gtcgctctgt gaggcacaaa aaatgactga
     6721 caaagacctc tacggcggtt tgatccgcct gcacatcctt caccatgcag ccgaggaacc
     6781 tgtctttggg ctggggatca tcgaagagct acgccgacac ggctacgaga tgagcgctgg
     6841 caccgtgtac ccgatgctgc acggcctgga aaaaaaaggc tatctgacct cacgccacga
     6901 acgcaccggg cgacgtgaac ggcgtgttta tgacatcact gaacaaggca gaactgcact
     6961 tgctgatgcg aaaacaaagg tcaaggagct gttcggagag ttggtagaag gtggttgagt
     7021 ttttgcttct atgtcaggtt gagctatacc ctaactggat tgtcattttc agaagacgac
     7081 tgcaccagtt gattgggcgt aatggctgtt gtgcagccag ctcctgacag ttcaatatca
     7141 gaagtgatct gcaccaatct cgactatgct caatactcgt gtgggctctg ttgcaaaaat
     7201 cgtgaagctt gagcatgctt ggcggagatt ggacggacgg aacgatgacg gatttcaagt
     7261 ggcgccattt ccagggtgat gtgatcctgt gggcggtgcg ctggtattgt cgctatccga
     7321 tcagctatcg cgaccttgag gaaatgctgg cggaacgcgg catttcggtc gaccatacga
     7381 cgatctatcg ctgggtccag tgctacgccc cggagatgga gaagcggctg cgctggttct
     7441 ggcggcgtgg ctttgatccg agctggcgcc tggatgaaac ctacgtcaag gtgcggggca
     7501 agtggaccta cctgtaccgg gcagtcgaca agcggggcga cacgatcgat ttctacctgt
     7561 cgccgacccg cagcgccaag gcagcgaagc ggttcctggg caaggccctg cgaggcctga
     7621 agcactggga aaagcctgcc acgctcaata ccgacaaagc gccgagctat ggtgcagcga
     7681 tcaccgaatt gaagcgcgaa ggaaagctgg accgggagac ggcccaccgg caggtgaagt
     7741 atctcaataa cgtgatcgag gccgatcacg gaaagctcaa gatactgatc aagccggtgc
     7801 gcggtttcaa atcgatcccc acggcctatg ccacgatcaa gggattcgaa gtcatgcgag
     7861 ccctgcgcaa aggacaggct cgcccctggt gcctgcagcc cggcatcagg ggcgaggtgc
     7921 gccttgtgga gagagctttt ggcattgggc cctcggcgct gacggaggcc atgggcatgc
     7981 tcaaccacca tttcgcagca gccgcctgat cggcgcagag cgacagccta cctctgactg
     8041 ccgccaatct ttgcaacaga gcccgccgtg ctagtctgct cggtgatggt ggagtgaagc
     8101 caacccgcaa tcgggttatg aatctgcatc gcgattcgca atcagctgtc tcttgagcat
     8161 gtcgaactcc tgcgatgtta tctcgccgtg ctcgtgctgc tacaccaact tgtccaactc
     8221 atccgccaca ccgctcccca ccacctgcga tggccaggtt gtttgctggg cgcattggcg
     8281 gagtatcgtc tcgacccggg acacccacac cggcacaacc ttacgggagt gattcactgt
     8341 caaagaatcg gcccggtgct ctgacgcaag tatcgggatg gtcaccattt gtaagccgta
     8401 gacctgagtg gtgatcaaga cttcgatacc accgaccgta ccggtactaa tcgacgacgg
     8461 tcgtattcgt cgcctgccgc agggactctg cacacctccg tttacgcatg tgcctggagg
     8521 agttggaaat cgtcgtgttc gggaaacatt aaacacagga tggcagcgat ctgagccagc
     8581 acatgatcag ctagctcacc atccggatcg acggcccact gcatcgtcgc gccagcgatg
     8641 accgagtgca ggagcaactc agctgccgca ggagcacctg ggggcagtcg cttgcggatc
     8701 ccctccacca ccgcgcggtt ccgctggatc gcaagcgtgc gtagctccgg cacctggagc
     8761 tcgtaccagg agatgagata gttcaccgag aagtcgttgc gagtgttcat gctccgaacg
     8821 agcacctgca aaaattccca gagcccttgc ggccctgcgc ctatcggtat cgcattcagg
     8881 taatgccgca cctgctcgac gccgcgctcc atcatcctca ccagcagcgt atcgcggttg
     8941 gtgaagcgct ggattaacgc tgcgcgggag agccccacct cctttgctac tccgctgagc
     9001 gtgaactcta tgggaccgca acgcttcagc actacggtgg cggcctcgag tacctcgtca
     9061 tcggacttga gcttggggcg gggcatcagt gttcaccttc tgtatgggtt ggggcggagg
     9121 ctgtggctgc cgccgccatt gtagcaaatt gaagacggag cgagagtaga gccacgagcc
     9181 ccgcaaacac ggccgataca acgaggccag ggagcggacc agcaaggtcg acaaacgcgc
     9241 cggccgcaag cataaccatg ggcgaggctg acagcatcac cgccgagacc gtgccgagta
     9301 cccggccgag aagttctggc ggcgtgcggt tgtagatggc agcgttgaga atgggagaga
     9361 ctgagccggt cagcagtccc acgagcgcgc ccaacaacat cagcaccggc acgcctggca
     9421 actgtgaaag cagaagcgag cccaccgcag agccacaaaa tgccaccgcc agccagttct
     9481 gcgctgatat ccgggcgccg accgacgcat gaatggcaat gccaaggaga ccaccagccc
     9541 ccatcattga ggagaacagc ccgagctctg ctacttggcg tcctgcatct acaaacagcg
     9601 caggcatgat gacgctgccg ttggcgccaa cgatgcccac gaagatcatc actataccaa
     9661 agagagggcg cagcaggggt tcgctccaga gaaaagcgac gccggcgcgc atggagagag
     9721 tcgccgtcgt ggtcatcgtc cgagcggcac gcgcgggaag cacccacgcg ccgagcagac
     9781 ctgcaaggac ggagcagaac gccgtcagcc cgagcgttgg cgcagcgcca agcaggccga
     9841 ttgcggcccc cccaagggcc gggccaccta gaatcgcgac gttcccgatc accgctttca
     9901 gtgacgagac gcgctcaacg gagagcccgg cgacgtggcc gagtttgggc agctcactgt
     9961 cctgcgcggc cataccgggt gcgtcgaacg cggcaccgag caccacgcaa gcgatcagcc
    10021 cagtgttcga gagggcgcca acggcatcga gcagtgggat gctcgccatg gccacgccgc
    10081 ccaccacacc cgagatcaat gcgacgggcg cgcgcccgaa ccgatcgacg aggccaccac
    10141 caacccacgc gccgatgatg gtcgcgatga cgctgctagc ggccgtggcg cccgcccagg
    10201 ccgcgctctt tgtatgagac aggacgaacc atggaagcgc gagggccgcc accgcgttgc
    10261 cgatccggaa gagaaaggtc gccgcgaaca gcgtcgcgag cgggctatat cgacgttcgc
    10321 tcattccgct gcggcgagct gcgccttcgc cgcagcgagg tactcttcgt tacccgagtc
    10381 gagggcgaag agtgcgtagg tgaccgcccc gaacgcaagg cgctccgcga tgtggtgggc
    10441 gagccgcggc cacacccggc caccggccgc ttcatacgtg aggaggagct tcgcgagccc
    10501 ctcttcacca aagaccataa ggtgcgcggc catgtcgatg gcagggtcat caacgcgggc
    10561 ctcgctccag tcgatcatcc cgctgacgcg ctccgtgttg tcgatgagca catggcccac
    10621 gtagagatcg ccatgcacca ccacggagaa atctggccac gacgaatcgt cgtcgagcca
    10681 gcgctgccac cggtggaggc gcttgtcgtt caccacgaac tcgcgtcgga cgcggtcaac
    10741 gtcgtcggcc accttctgac gggcctgcgt cggtgtacgg atgagcatcc ccgcatccac
    10801 ggcggcggaa atggggacgg catgcagggc ggcgagcgcg gtcgcgaagc tctccgcgaa
    10861 gacctccgag tcctgcggca cgacccagtc gggcgtggac gaaccaggct ggatgaccat
    10921 cgcagtcgag tcttcgagca tgggataggc aacgagctcg gcgttggcca cgcgccagtc
    10981 cggcaccgcg aacggcaggc gattcttgag cattgccagc acccgcgcct ctggttcgac
    11041 cttcgcgctt acctcggctc ggcgcgggat gcgcagcacc caccgacgtc catcgtcgac
    11101 ggtggcgatc acgatcctat agtcgagccc aagctcattg acagtcagcg ggccatggag
    11161 cttgagccca tgtcgggctg caagtgcgta cagttgggag gtatcggcgg tcgtgactac
    11221 ggtcatgatt cactcctgag ggcttgacgg gtttagccac ctaaatgtaa cagtcacgtc
    11281 ggttatattc aatccggcac tgttgcaaag ttagcgatga ggcagccttt tgtcttattc
    11341 aaaggcctta catttcaaaa actctgctta ccaggcgcat ttcgcccagg ggatcaccat
    11401 aataaaatgc tgaggcctgg cctttgcgta gtgcacgcat cacctcaata cctttgatgg
    11461 tggcgtaagc cgtcttcatg gatttaaatc ccagcgtggc gccgattatc cgtttcagtt
    11521 tgccatgatc gcattcaatc acgttgttcc ggtacttaat ctgtcggtgt tcaacgtcag
    11581 acgggcaccg gccttcgcgt ttgagcagag caagcgcgcg accataggcg ggcgctttat
    11641 ccgtgttgat gaatcg
//
LOCUS       DAZSNM010000126        11655 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZSNM010000126 DAZSNM010000000
VERSION     DAZSNM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.126"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..794
                     /locus_tag="ACHW42_002781"
     CDS             <1..794
                     /locus_tag="ACHW42_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shiE"
                     /protein_id="HFR8944529.1"
                     /translation="SETKAAYRCFSNDKVSANKIMTPHKENITERAQQFKRVLVLQDT
                     TELNYSGQKEKQGVGPKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKN
                     TRQEICNDLLHKKHVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFER
                     AAQTPGIKADWLVRMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQ
                     ARTLPDMLSSDAGPLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="ACHW42_002782"
     CDS             complement(744..1937)
                     /locus_tag="ACHW42_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8944530.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="ACHW42_002783"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="ACHW42_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR8944531.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="ACHW42_002784"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="ACHW42_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR8944532.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="ACHW42_002785"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="ACHW42_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR8944533.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="ACHW42_002786"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="ACHW42_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR8944534.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="ACHW42_002787"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="ACHW42_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR8944535.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="ACHW42_002788"
     CDS             complement(10573..10716)
                     /locus_tag="ACHW42_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944536.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10955..>11655
                     /locus_tag="ACHW42_002789"
     CDS             10955..>11655
                     /locus_tag="ACHW42_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR8944537.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQW"
BASE COUNT         2956 a         2897 c         2992 g         2810 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggcaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tggga
//
LOCUS       DAZSNM010000127        11628 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZSNM010000127 DAZSNM010000000
VERSION     DAZSNM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11628
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.127"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1038
                     /locus_tag="ACHW42_002790"
     CDS             <1..1038
                     /locus_tag="ACHW42_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR8944538.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1039..3204
                     /locus_tag="ACHW42_002791"
     CDS             1039..3204
                     /locus_tag="ACHW42_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFR8944539.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3218..4129
                     /locus_tag="ACHW42_002792"
     CDS             3218..4129
                     /locus_tag="ACHW42_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFR8944540.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4129..5424
                     /locus_tag="ACHW42_002793"
     CDS             4129..5424
                     /locus_tag="ACHW42_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFR8944541.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5469..5699
                     /locus_tag="ACHW42_002794"
     CDS             5469..5699
                     /locus_tag="ACHW42_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944542.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5677..6177
                     /locus_tag="ACHW42_002795"
     CDS             5677..6177
                     /locus_tag="ACHW42_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFR8944543.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6177..7595
                     /locus_tag="ACHW42_002796"
     CDS             6177..7595
                     /locus_tag="ACHW42_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFR8944544.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7595..8443
                     /locus_tag="ACHW42_002797"
     CDS             7595..8443
                     /locus_tag="ACHW42_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFR8944545.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8443..8898
                     /locus_tag="ACHW42_002798"
     CDS             8443..8898
                     /locus_tag="ACHW42_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFR8944546.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8901..9593
                     /locus_tag="ACHW42_002799"
     CDS             8901..9593
                     /locus_tag="ACHW42_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR8944547.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9603..10934
                     /locus_tag="ACHW42_002800"
     CDS             9603..10934
                     /locus_tag="ACHW42_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR8944548.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10935..>11628
                     /locus_tag="ACHW42_002801"
     CDS             10935..>11628
                     /locus_tag="ACHW42_002801"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR8944549.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLT"
BASE COUNT         3115 a         2904 c         3055 g         2554 t
ORIGIN      
        1 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
       61 gatggcattt caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt
      121 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt
      181 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
      241 atgtctgacg ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac
      301 atgaccatct atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca
      361 tatcccgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
      421 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
      481 tacgtaattg gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg
      541 tgggataagg aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
      601 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct
      661 catgatggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac
      721 gctggattta tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag
      781 ccaggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc
      841 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
      901 aagcttaacg acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca
      961 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
     1021 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt
     1081 gacgcagact ggatggcgag cgatgaagcc agaaccgagg cgacaaatga cctgtatttt
     1141 agccgagtgt cgcaatggga tgactggcta tcaaactaca ctaccctgca atatcgcgga
     1201 caattcgatg ttgttcgccc ggaggtcagg aaactggtcg cagagatgcg ccggaaccct
     1261 gtcgacgttc tcttccgacc caaagacggc gctaatcctg atgcagccga tgtattgatg
     1321 gggatgtatc gtactgatat gcgccataac acggcaaaga ttgccgttaa cgttggcgtt
     1381 cgtgagcaga tagagtcagg cgttggtgca tggcgtctgg tcacgcagta cgaagacaac
     1441 gatccaacaa gcaacaatca ggtaatccga cgcctgccaa tccatgaagc ctgctcacac
     1501 gtcatatggg acgccaacag caagcagatg gataagagcg acgctaagca ctgcacggtg
     1561 attaacgcct tgtcgcgcaa tggctggaaa gagttcgcag aggattacgg tattgacccg
     1621 gataccctgc catctttcca gaatcctaac gatacatggc tattcccgtg ggtgtcgaat
     1681 gatgtcgtct acgtcgctga gtattacgag gtagaagagg agaaggagaa ggtcttcatc
     1741 taccgcgacc cgctgacagg tgagccggtc agctattacc agcaggatat caaagacgtc
     1801 atcgacgacc tggctaatcg tggattcatt aaggtagcag agcgcaaggt gaagcgtcgg
     1861 cgtgtgtata agtcgatcat cacctgtacg cagatactga aagaccgcga gaagatagcc
     1921 ggagagcata ttccaatcgt tccagtgtat ggcgaatggt cattcgctgg tgacaaggag
     1981 tgctacgagg gtgtggtaag gctgacgaaa gacggtcaac gccttcgtaa catgattatg
     2041 tcgttcaacg ccgatattgt tgctcggtca ccgaagaaga aaccgacctt cttccctgag
     2101 caaatcgaag gctacgaata catgtacggc ggaaatgatg actatccgta ctatctgcag
     2161 aacaggactg atgaaaacgg taacgacctg ccgattggtc caatctccta catggaaaac
     2221 cctgaagtgc cgcaagccaa cgcttacatg cttgaggctg ccaccaacgc agtgaaagaa
     2281 gtggctagtc ttggcgtgga tgcgcaggcg gcaaatggtc aggtcgcttt tgataccgtc
     2341 aatcaactga acatgcgggc agaccttgag acatacgtgt ttcaggataa cctggctacc
     2401 gcaatgcgac gtgatggcga gatttatgcc tcaatggtca acgatattta tgacgttcct
     2461 cgtcatgtaa cgctgacact tgaagatgga agcgagaaag acgttcaact ctatgcgcaa
     2521 gttgtcgatt accagtccgg caatgtggtc acactcaacg acattcgcgg tcgatatgag
     2581 tgctatacag acgttgggcc atccttccag agtatgaagg aacagaaccg cgcagagatt
     2641 caggagttgc tcaccaaggt tccgcaaggt actccagagt tccagatgct gatgctgcaa
     2701 tacttcacgc tgcttgacgg taaaggcgtc gagatgatgc gagagtacgc gaacaagcaa
     2761 ctggtgatga tggggctgaa gaaaccagaa acacctgaag agatggagat ggtgcagcag
     2821 gcacaacaac agccgcagca gccatcagca gagcaaattc aggcgcaggg tatccttctg
     2881 caaggtcagg ctgaattgct caaggcagag aaccaacagg cgcagattca ggttgaagct
     2941 gccaaggttg aagcccaaaa ccaactcaac gccgcgaaga ttgcagaaat attcaacaat
     3001 atggacctcg acaagcaggc agaactgcgt gagtacctca ggctcgtagg tcaattccag
     3061 caacagcgca gcaaagatgc tcgtgctaac gctgagctgc ttcttaaaga tgcagaccag
     3121 actcattcac aacgcatgga tttcgcgaat cttatgcgtc aagttcaaat cccctccggc
     3181 ggagtagccg agacacctca ataagagaga gttaatcatg gaccaaacca ccgacattca
     3241 ggcttctgaa gaattaaccc tgcccggcaa tcatgcagcg gcatctgctg atggcttagt
     3301 tgtcgataat gccaacgaca gcgcaggtca ggaagaaggc tttgagattg tccggaaaga
     3361 cgatgagaaa ccaaaacaag acccggcaac taatgctgaa tttgcccgtc gccgcatcga
     3421 acgcaaacgc cagcgtgagc ttgagcagca gatggaagcg gttaagcgtg gagagttgcc
     3481 ggagcacctg cgggtgaacc ctgagttacc aaaacaacca gaccctaacg attatctttc
     3541 cgaagatgca ctggctaagt acgactatga ccagagccgc gcactggctg ccttccagca
     3601 ggcaaacagt gaatggcaga tcaaggctat ggacgcacga agccaggctg tcgccgagca
     3661 gggtcgcaaa actcaggagt tcacccagca atcagcgcaa tacgtcgagg cggcccgtaa
     3721 gcactacgag gcagcggaaa agctcaatat ccctgactat caggagaaag aggatgcatt
     3781 catgcaactg gtgccgccag cagtcggtgc cgacatcatg cgcctcttcc cggagaaatc
     3841 cgccgctctc atgtatcacc ttggtgctaa tcctgagaaa acacgccagt tgctggcgat
     3901 ggacgggcaa tccgcgctga ttgaactcac tcgactgtca gaacgtttaa ctctcaagcc
     3961 tcgagccaag cctgtttcag aagccccgtt acctgatgaa cccattcagg gacatgctgt
     4021 tgctgcaaat atctctgcga ttgaaaagca gatggaggcg gcagcaaaca aaggggatgt
     4081 agagacgtac cgcaagctca aggcgcaact gaataaagga attcgataat ggcattaaat
     4141 gaaggtcaac tggtcacgta tgctctggat gaaatcatcg gaaccgtcca gaacctgacg
     4201 ccaatggcgt caaaagtgac aaaatacccc cctccggcag aatccatgca gcgttcaagc
     4261 aacaccgtgt ggatgcctgt tgagcaggaa gcgccaaccc agactggctg ggatttaact
     4321 ggcaacgcaa ccgggattct ggaactctcc gtgaagtgca acatgggcga tccggataac
     4381 gatttcttcg agcttcgtgc agatgacctg cgtgatgagc gttcttaccg tcgccgcatc
     4441 caggcatccg ccaaaaaact ggcgaataac attgagtcag cgattgccaa acaggcaact
     4501 gaaatgggct cgcttgttgt tcacgatacc cgcgcaattg gtccatcgac tggcctgcct
     4561 ggctgggatt ttgtgtctga tgcagagcga ctgatgttct cccgtgagct aaaccgcgac
     4621 atgggcatca gttacttcct gaaccctgac gattaccgca aagcaggccg caacctggta
     4681 gatggtgaca tcttcgggcg cgttcctgaa gaagcatatc gaaacggtac tattcagcgt
     4741 cagattgctg gctttgatga aattcttcgc tcaccgaaac ttccggcagt taccaagtca
     4801 accgcgactg gtgtaactgt ttctggtgcg cagaagttta agccgcaggc atacaccctt
     4861 gataccgatg gtaacaaaga gaacgtcgac aaccgtgttg caacggtgac cgtatcctcc
     4921 accaccggat ttaagcgcgg cgacaaaatc agcttcattg gtgtgaagtt cctgtctcag
     4981 atggcgaaga atgtgctgac tgatgacgcg actttctcaa tcacccgtgt gatcgatggt
     5041 actcacatcg aaatcacgcc gaagccgatt gcgctggatg acgcgtcact gacaaaagaa
     5101 gagaaggctt acgctaacgt aaacacctct cttgctgata ccactccggt aaacgttctg
     5161 aacgtggcaa caaccaccgc taacgtgttc tgggctgatg actcaatccg cctgctgtct
     5221 cagccgatcc cggtaaccca tgaactgttt gctggcatga aaacgtcttc cttcagcatt
     5281 ccaggtattg gtgttaacgg catcttcgca acgcagggtg atatcaacac tctgtctggt
     5341 aagtgccgta ttgctgtgtg gtattcagca tgtgctgtac gaccagaggc aattggtgtt
     5401 ggtctgccta accagaccgc gtgataacca gagggagctt cggctcccgt ttttatctgg
     5461 agacaagcat gacacacatg atctttcgtc atggcgacat gaagaagtgg aaaggcgttg
     5521 gctacgactt tgaaatcgtg aaagccgaag agcttcagga atatctggat gctggctggt
     5581 tttcacatcc tgatgacctt ttgaaggatg ttgcagagcc agagccagaa gaaaaacagc
     5641 gtaaaaagcc tggtcgaaaa cctaaggcgg cagcagatga acctgacaac gaagggtgat
     5701 ttagttcttg cggcattacg taagctcggt gttgcatcaa atgccacgtt aaccgatgtc
     5761 gaaccgcagt ctatggaaga cggcgtcaac gaccttgaaa tgatgatggc tgaatggctt
     5821 ggcggtgatg cgtcacctgg tatcaacgtt ggctacattt ttgctgatgc agatgttgct
     5881 ccggatccgg gcgatgagca cggtttatca aataacgcta tcaatgccgt cattttcaac
     5941 cttgcctgcc gaattgctcc agattatgcg ctggaagcgt ctgcaaaact tataaccact
     6001 gccagatacg ggaaagagcg actcgtcaaa ctgtctgcaa tggacagggc aaaagcagct
     6061 aaatgtaagt ccggttatcc aaaccgtatg cctgttggta gtggtaacca gttggcgaag
     6121 tggaatggtt ggaattactt ccaccgaaag gaaccttgcg ataacgggag cgaataatgc
     6181 ctattcagca acttccgctt atgaaaggtg tcggcaaaga cttccgaaac gccgactata
     6241 tcgactatct gccagtgaat atgctggcta cacccaaaga aatcctgaac agcagcggat
     6301 atcttcgctc attcccgggc attgccaaac gttctgacgt gaacggtgta tctcgcggcg
     6361 tcgagtacaa catggcgcag aatgctgttt atcgcgtgtg tggcggcaag ctgtacaaag
     6421 gagaaagtga agtcggtgat gttgccggaa gtggtcgtgt atcaatggcg catggtcgga
     6481 catcacaggc ggtaggcgtt aatggtcaac tggtcgagta tcgctatgat ggcacggtca
     6541 aaaccgtctc aaactggcct acagacagtg gattcactca gtatgagtta ggttcagttc
     6601 gcgacattac gcgcttacgt gggcgttatg cgtggtcaaa agacggaact gattcatggt
     6661 ttatcactga ccttgaagac gaatctcatc ctgaccgtta cagcgcacaa tatcgtgccg
     6721 agtctcagcc tgacggcatc atcggtatcg ggacatggcg agacttcatc gtctgctttg
     6781 gttcatcgac tattgaatat ttctccctga ctggcgcaac caccgttggt gccgctttgt
     6841 atgttgcaca accatcgctg atggtgcaga aaggcattgc cggaacttac tgcaaaacgc
     6901 cattcgctga ttcgtatgcg tttatcagca atccggcaac gggtgcgccg tctgtgtata
     6961 tcatcgactc cggtcaggtg tcaccaattg ccagcgcgag cattgagaaa atcctccgct
     7021 cctacactgc tgatgaactg gctgatggtg tgatggaatc gctgcgattt gatgctcatg
     7081 agttgctgat tatccacctt ccgcgtcacg tcctcgtgta cgacgcatct tcaatcgcca
     7141 atggcccaca atggtgtgtg ctgaaaacag gtctgtatga cgatgtgtac cgcgctatcg
     7201 acttcgttta cgaaggcaat cagataacgt gcggcgataa gctggaatct gttaccggga
     7261 aactgcaatt cgatatcagc agccagtacg acaagcaaca ggaacacctg ctgtttactc
     7321 cactcttcaa agcggataac gccagagtgt tcgaccttga ggttgaatcg tcaactggcg
     7381 ttgcgcagta cgccgaccgc ctttttctct ctgcaaccac tgactgcatc aattacgggc
     7441 gtgagcagat gattgagcag aatgaaccgt tcgtttacga caagcgtgtt ttgtggaagc
     7501 gagtagggcg catcaggaaa aatgtcggct tcaaattgcg cgttatcacg aagtcacctg
     7561 tcactctgtc tggcgctcag ataaggattg agtaatggct gattcgaatc tcaatgtgcc
     7621 ggtaatcatc caagctacgc ggctcgatac atcagtcctt ccacgcaata tcttctcgca
     7681 gtcgtatctg ctttacgtta tcgcacaggg cactgatgtt ggtaacgtgg ctaacaaagc
     7741 caacgaggcc ggacagggcg cttatgatgc acaggtcagg aacgatgagc aggatgtgat
     7801 tctcgctgac catgagcagc gaatttctgc tgcggaagca acgcttgtta atcatgagga
     7861 gcgaatcaga caggcagaat caactcttca ggaccatgaa acacgaatag ctcagaatga
     7921 aagcgatatt tcgtcgcttg acacaagagt tcagtcgctg gagtcgcagg tttcagacca
     7981 tgaaacgcgc atcgatgctc tggagtatgc aaccacacgc aagaagtcag aggttgttta
     8041 ctctggtgta tctgtaacca tcccgacagc gccgaccaac cttgttagcc tgctgaaaac
     8101 gctcacaccg tcatccggct cgttggcacc attcttcgac accgttaaca acaagatggt
     8161 tgtgttcaac gagaacaaaa ccctgctctt caagttgtcg attgtcggca cgtggcccag
     8221 cggaaccgcc aacaggtcaa tgcagctaac cttttccggc tctgttcctg acacactggt
     8281 cagcagtcgt aatgcggcga caacaaccga caacatcctg ttagctacgt tcttcagcgt
     8341 ggataaagac ggctttcttg ccacaaatgg cagtacgtta accattcagt cgaatggtgc
     8401 ggcgtttact gccacaacca tcaagataat cgcggagcag tgatgattac attcaaacca
     8461 acgcgaaaca tcgacctgat agaagcagtc ggaaatcacc ctgacattat cgccgggagc
     8521 aacaacggtg atggatacga ctacaaacct gaatgccgtt actttgaggt gaacgtgcac
     8581 ggtcagtttg gcggcattgt ttactatcag gagattcagc cgctgacatt agattgccac
     8641 gccatgtacc tgccagagat tcgcggcttc agcaaggaaa ttgggctggc gttctggcga
     8701 tacattctga ctaacaccac cgttcagtgc gtaacatcgt tcgctgcacg caaattccga
     8761 cacggtcaga tgtactgcgc aatgattggc cttaagcgtg taggaaccat caagaaatac
     8821 ttcaaaggcg tggatgacgt gactttttac agcgccacac gcgaagaact aatcgacttc
     8881 ctgaatcacg ggagatagcc atgttatatg catttaagct gggcagaaaa ctgcgcggcg
     8941 aggaacctta ttgccctgaa aaaggcggga aaggtggcag ttctgataaa agcgcaaagt
     9001 atgcagcaga agcccagaag tatgctgcag acctgcaaaa tcagcagtgg cagacgatca
     9061 tgaaaaacct tgctccgttc acgcctcttg cggagcagta tgttaaccag cttcagaacc
     9121 tttccagttt agaaggtcag gggcaggcac ttaatcagta ttacaactct aagcagtata
     9181 aagaccttgc agggcaggcg cgttaccaga gtcttgctgc tgcggaggcg acgggtggac
     9241 ttggttcgac agccacaagc aatcaactgg ctacgatcgc gccgacactc ggtcagtctt
     9301 ggttatcaaa tcagatgagc aattacaaca atctggcaaa cgttgggctt ggtgcgctgc
     9361 aaggtcaggc aaacgccggg cagacgtacg ccaacaacat gagcagcatt gcacagcaaa
     9421 gcgcagctct tgccgctgct aatgccaata aaccatcaag tcttcagact gcaattagcg
     9481 gtggcacgtc tggtgcgatt gccggtgcag gtcttgccag ccttttggga acatcaacgc
     9541 cttggggcgc tggtattggt gctggtatcg gattgcttgg ctcgttgttt taagggggaa
     9601 tcatggctac ttggcaagga acaaacggcg gattgttggc tggtatcggc ggcgtcaact
     9661 caaacgctcc gagcgtaaat gacatcggca atacgcttca gcttatcagg cagaacaatg
     9721 atattgagcg ttcaggcgct aacaatgttg ggctgactgc tttgcaaggt ctttcaggta
     9781 ttgcgggtgt ttttcagcag gaaaagcagg ctcagcggca gaaagaattt cagcaggcgt
     9841 acgctaatgc ttatgcgtct ggtgatcgcg gtgctttgcg tcagttggct actcaatatc
     9901 cagaccagat tgaatccgtt cgtaaaggca tgggattcat tgatgaagag cagcgtaatt
     9961 ctatcggcac cttagcggct ggcgcacgcc ttgcgtcatc gtctccagaa gcaatgcaat
    10021 catggctgca aaacaacgcc ggtgagttag ctcgtgttgg cgttaatcct catgacgtcg
    10081 ctcagatgta ccaacagaac ccgcggcagt tcggcgaatt tgtcgatcac ctggggatga
    10141 acagtctcgg gcccgaaaaa tactttgacc tacaggataa aatgcagggt cgccaggtta
    10201 ccatgcgcgg tcaggatctg gattcgcaaa ccgccgctcg gaatcaggca atcacaatgc
    10261 gcggacaaga tatccaggcg aatttaggtc agcagcgcat taatctggac gcagaaacaa
    10321 accgcattaa caacgaaaat aagcgccttg accggatgct atcagcagaa actaacgacc
    10381 tgaagcgcca ggaaatacag agccgcatag cagccaacaa ccagcagttg cagcagaagc
    10441 agcaagcgct aaatgatggc tacaaagacg gcatcaacac cctcacaacc agcatgttca
    10501 ctctgaacga tatcgttagt tctccttcac ttaagagcat tacaggctta cgtggagtaa
    10561 tccccaacgt tccaggctca caggctgcag acactcaggc acgacttgat acctttaaat
    10621 cacaagcata cctgacagcg gttcaggcca tgcgaggcat gggcgcactt tctgatgccg
    10681 agggcaaaaa gctcgaccag gctgttggtt cgctgcagaa ctcgcagagc gaggagtcct
    10741 ttcgtcgcaa cgctggcgtc atcctgaaca cgctcaacca gaagcgtaat gaggcggttg
    10801 gtaagtacgt tcagcaaaac ggtatcaagc gagtggaagc gcctcaggct tctatagatt
    10861 acctgaagca gcaccccgag ctgtcaatcg acttcattaa tcgctacgga tatcttccat
    10921 ctttggggca gtaaatggct aattaccgtg atttgttaga gcaggctggc gcacgttacg
    10981 gtgtgccaga agggttgatg actgcactgg gtgccaagga gtcttcttac aaccctgccg
    11041 cagtaagctc cgccggggct gtaggattga ctcaggtcat gcctgggaca tggcgtgata
    11101 tgggttatac cgatgagcaa atgcaaaacc ccgaatatca ggctgacgct ggcgcgcgct
    11161 atctggcaaa gatgtaccag cagtttggta actggcgtga ctctcttcag gcttatcacg
    11221 acggtcccgg caacgttatg aaggcaaagc gtggtgaata tacgccagga cctgaaggcc
    11281 gcggttacgt tgatgatcgc tttgctcaat gggctggtga cccggtgaca gactcaacag
    11341 tcgaacagcg cgccacctct gcaaaggtac atcctcagca agaccctaac aacccgtttg
    11401 cacaactgga agtacagtca tccgaacaag tatcggcatc aggtgtgcag tcagacccaa
    11461 ataatccatt tgctcagatt gagcagcagg cagccagtca gcagccacct caacccgtaa
    11521 gttctgtcgc accgaaacct gttcagcaac aaacagttaa tcaggccaat aatgcaccaa
    11581 cacgggaaga accatcattg atgcaacaag ctggcgattg gctcactg
//
LOCUS       DAZSNM010000128        11478 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZSNM010000128 DAZSNM010000000
VERSION     DAZSNM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11478
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.128"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..422)
                     /locus_tag="ACHW42_002802"
     CDS             complement(<1..422)
                     /locus_tag="ACHW42_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8944550.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVAN"
     gene            complement(548..3691)
                     /gene="sbcC"
                     /locus_tag="ACHW42_002803"
     CDS             complement(548..3691)
                     /gene="sbcC"
                     /locus_tag="ACHW42_002803"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR8944551.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(3688..4890)
                     /gene="sbcD"
                     /locus_tag="ACHW42_002804"
     CDS             complement(3688..4890)
                     /gene="sbcD"
                     /locus_tag="ACHW42_002804"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR8944552.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            5080..5769
                     /gene="phoB"
                     /locus_tag="ACHW42_002805"
     CDS             5080..5769
                     /gene="phoB"
                     /locus_tag="ACHW42_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR8944553.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            5827..7122
                     /gene="phoR"
                     /locus_tag="ACHW42_002806"
     CDS             5827..7122
                     /gene="phoR"
                     /locus_tag="ACHW42_002806"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR8944554.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            7529..8848
                     /gene="brnQ"
                     /locus_tag="ACHW42_002807"
     CDS             7529..8848
                     /gene="brnQ"
                     /locus_tag="ACHW42_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR8944555.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            8924..10297
                     /gene="proY"
                     /locus_tag="ACHW42_002808"
     CDS             8924..10297
                     /gene="proY"
                     /locus_tag="ACHW42_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR8944556.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            10453..>11478
                     /gene="malZ"
                     /locus_tag="ACHW42_002809"
     CDS             10453..>11478
                     /gene="malZ"
                     /locus_tag="ACHW42_002809"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR8944557.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHML"
BASE COUNT         2413 a         2932 c         3141 g         2992 t
ORIGIN      
        1 aaattggcga ctgtcatccc ggaaaccatc cccgccacgg cggcggtgac ttttccgggt
       61 ttgataattt ttgataacac gatcgctccg acgccaaaaa atgcgccatg cggaaagccg
      121 gataccagcc gaccaatggc gagcatcagg taagacgaag agagcgtgaa catggcgttg
      181 ccaatgacgc acaacgccac cagaaacaac aagatatgtt tgagtgagta gcggctggaa
      241 aagagtgcga tgattggcgc accgaccacc accaccagtg cataatacga gatcatatgc
      301 ccggcagcag gaatcgaaat tcctacgtta tgcgccagct ccgtgagcac gctcataatg
      361 ccaaattcgg ccatccccaa accaaacgtg cccagagcca aagataaaat gacttttttc
      421 ataccaccat cacaaccacc agaacggcta ccaatcaccg tagccaataa agtgatagct
      481 tgcaggataa cgaccagcaa ggccaagaga taattcaccc ctctgtattc attatcctgc
      541 tgaatagtta tttcactgca aacgtacttt ccagtttgct gtagcccagg ccgttgatct
      601 ttttcacttt gatctgcacc ggaatacgct ctttcatcgc ttctacgtgg ctaatcacac
      661 cgatggtttt gccactggcg ttcagggcat ccagcgcatc aagggcggta tccagcgttt
      721 cgctatccag cgtgccaaaa ccttcatcaa ggaacagcga gtcaatacgt gttttatggc
      781 tgaccagatc cgaaagcgcc agcgccagcg ccagactaac gaggaaactt tcgccgccgg
      841 aaagggtacg ggtatcgcgt accgcatctg cctgccaggt atcaacaacc tcgacttcca
      901 gcgcctcgct ggctttgcgc tgtaacagat agcgcacgtg cagccgggta agttgctgat
      961 tagcgagatg gactaaatta tccagcgtca gcccctgggc aaacttgcgg aatttatcgc
     1021 cctctttgga acctattagc gaattcagat atccccagtc ctcaacctgc tgcgtcattt
     1081 gagcaatttg ctgcattaag gtttgttgtt gctgacggtt atctgcatcc tgcttcagct
     1141 gctggcgaat ctcgccttga ctcgtggtgt tttcacgcaa cttttggtga gtttgcgcta
     1201 actcttgctg aatctgctcc accgtcacag tgagagccaa cccgtcaggt cggtgttgtt
     1261 gatgctgtgc cagcgtttct gctgtctgag tgaccagcgt ttgcgcctga cggcgctggt
     1321 tttccagatt ctgcttgagc tgttccagct gcgttagtgt ttgttcatcc attagcgccg
     1381 caaggaacgc ctgctgatcg tcaaagacgc tggcctgtag cgcggtgtca aactgcgctt
     1441 gggctttttg cagactttgc gccgccagaa catcctgttg ctgtaacgtc tgctgctggc
     1501 tgtgtaatgc gagacattgt tcatgaacct gccgccagtt atccagcgcc acagtttctt
     1561 cgctgtgcgg gagatcatca ctttgcggca acgtttccag aatcggtgtc agctgctgaa
     1621 tacggttttg cagcgcggtt aattcgttct ggcgttgctg ccagctctgc gcttcttgct
     1681 gacgtgtcgc caaccagctc tcttcttcat cttcctgtgg caatgtcagt gcataacccg
     1741 ccaatgccgt taagagctgc tgctgacgtt gttcaatttg ctgttgatac tggataattt
     1801 gctgattatg cgcggcaatc tgcccttgta attcatgtcg ttggctgagt aaccgcagct
     1861 ggcgttcgtg ctcatcttgt gcatccagcc acggttgaat atcgtcctgt ggctgcaagg
     1921 tgatattgag gctggccgtg acggcttgcc attgttgagt aagtgcttgc tcatcttgtc
     1981 ggaggctttg cgcttcgttt tcatcgcgct gaagctgctt cgttaatgca tccagttgcc
     2041 cgcgcagcgc cgcaccttct tcgccgagct ttttaacttc gttttccagc gccagtaatc
     2101 gagactgatt aacaccaggc tccagcgcct gatacgcctc gaccgccggg tggctggtgg
     2161 aaccacaaag tggacaaggc tgacccgcct gtaactgtgc acgctgggct tccagcgttt
     2221 tgatgcgcgc ttcctgctcg caaatggttt tcacatcggc aagttgctgc gtcttttctt
     2281 tataacgctg gcgcatttcg ttaagtgcga cattacgttg cgtctgttcc agagtgacat
     2341 tctggatagt gaccattaac tgcgccagac gtttttgttg gggaacaatc tgcccatgca
     2401 gcgcgaccag gcgctgacgc agtgggcgtt gctcggcatg ttgcgccagg gcggtagcaa
     2461 cttcatcggc ggttaacgtc aacgtgatcg ccgcaagcgc attaagtttt tgctcagcat
     2521 gggttaactg ttgctgccat tgccgcagat gctcgcgatc gctggtttgt tgggagaact
     2581 gcgcacgcca acccgccagt tcgttgttcc actgacggaa gcggtcgtgt tcctgtaacc
     2641 aggtattcag gctttgttgc tgctgctgta attctgctga ctgcttcgcc gcgtggtggc
     2701 gaatgctcgc gcgaagcgcc attgtgctct gtaagcgagt atttacttct tcaatctgct
     2761 ggcgaatatg cgccagcgcc gcgctgtgtt ctgcgatgcg ttcccagtgt ggacgaagat
     2821 ttcgtgccgg ttgtgccaga ctaagcgccg ccagttgagg ttgcgctttt tcttcttcgg
     2881 ctaacgcctg ttgcaaggcc tgttgacggc ggctggcttc ttgctgcaat tcgtcctgac
     2941 gcgttaacca gtttagcgat tgttgttctt gctgctgcgc ggtaattaac tgtttttctt
     3001 cgtcagtaag tacctgcaaa ctcgctgtca gcgattgcac ttgttccggc gtgagcaacg
     3061 tgacgccgct ggcctgcgct tgcagcttct ccagctcagt gcgggccgat ttgtgctgct
     3121 caaaaaccat cgccgagatt tgcccgtaga tttcagtgcc ggttaactcc tcgagcaatt
     3181 ccgcgcgttc tttgggtttg gcattcagga aggcagcaaa ttgcccctgc gaaagcagca
     3241 tcgaacgggt gaagcgcccg taatccagcc cggttaacgt cgctgtcagt tccagcttat
     3301 ctttcacttt gtcggcgaga attttgccgt cggcgcagcg cgccagctct acgcgaggca
     3361 cctgcaaatt accgtccggc tggttacgcg cccgattctg gctccagaac gcgcgatacg
     3421 cttcaccttt cacttcaaac tccacctccg ccagacattc agcggtatcg cgggtcatga
     3481 gatcattttg cgattgtgaa acgttagaga gacgcggagt ttcgtgatac agcgccagac
     3541 aaatggcgtc cagcagggtg gttttccccg caccggttgg gccggtaatg gcaaacagcc
     3601 cgttgctggc gaacggctcg cgggtgaaat caatcttcca ttcgcctttt aatgagttca
     3661 ggtttttcag gcgcaggctg agaattttca tgcttcgtgt tctccggcga gggtacgcaa
     3721 cgtcgtggtg aaaagatgct gcagacgttg ctgctgcgat tcatccaggt cttccagtgc
     3781 cagacggcga ttgaacacct cttcgacgct gagttcgctg agggtttcac gctgttggct
     3841 ggctaacaca cgctcgcgct gttcacgact ccgacgtacc agcaatactt cgacaggcaa
     3901 tgattcggtt aatgcctgga ttttgcgctg aatatcatgc agatactcat cagtagtgat
     3961 ttcgatatcc agccagacag gtggctcctg cgatacatcg cgccactgtt ccagctgtgc
     4021 ggtaatcgac gccagatcgc ctttcagcac cgccatgggt tgagttaccg gtacgttcag
     4081 gttttccacg ctctctaatt tgccgtttga aaatgtcacc agatggacat atttactctt
     4141 accgcattca tcaaaactca gtggaatggg ggaaccgcaa tagcgaacat gttccatgcc
     4201 gccaataatc tgtgcgcggt gaatatgccc gagcgcgatg tagtcggctg gtggaaagtt
     4261 ttgtgccgga aacgcgtcca gcgtgccaat ataaatgtca cgcacggcgt cacttttact
     4321 ggaacccacg gtcgttaaat gtcccgtggc gatgatgggc agaggctgat cgccgcgcag
     4381 tttgcaggca tcggcatagt gttgttggta ataatcggta atcgctgcca gtaaatgctg
     4441 ctgtttttca ataccgttaa gccccgcctg gctggaaata atgtcacgcg ggcgtaaaaa
     4501 cggaatgggg cacagcactg cgcctggcgt cccgtcgcga cgaggcaaga tttgcggcgc
     4561 atgtccggcg ctggcgacca cggttgtatt gaggaacgcc atgatatcgc gcgattcatt
     4621 cagcgtggcg accgagtcat ggtttcctgc cagtaccacc agatgacagc cagtttgctg
     4681 taaattgaca acaaaacggt tgtataacgt gcgggcgtaa ctgggcggcg agccggtatc
     4741 gaaaacatca ccagcaacaa taatcgcatc cacctgatgg gtttgtgctg tctccagcag
     4801 ccagtcaaga aaagcctgat gttcagcttc gcggctttta ctgtagaagt tctggccgag
     4861 atgccagtct gaggtgtgaa ggatgcgcat aacggttccc tggcgaaaaa gcatggtcgc
     4921 gattataccc aaacagatgt gccatttgct tttttctgcg ccacggaaat caataacctg
     4981 aagatatgtg cgacgagctt ttcataaatc tgtcataaat ctgacgcata atgacgtcgc
     5041 attaatgatc gcaacctatt tattacaaca gggcaaatca tggcgagacg tattctggtc
     5101 gtagaagatg aagctccaat tcgcgaaatg gtctgcttcg tgctcgaaca aaatggcttt
     5161 cagccggtcg aagcggaaga ttatgacagt gctgtgaatc aactgaatga accctggccg
     5221 gatttaattc tccttgactg gatgttacct ggcggctccg gtatccagtt catcaaacac
     5281 ctcaagcgcg agtcgatgac ccgggatatt ccagtggtga tgttgaccgc cagaggggaa
     5341 gaagaagatc gcgtgcgcgg ccttgaaacc ggcgcggatg actatatcac caagccattt
     5401 tcgccgaagg agctggtggc gcgaatcaaa gcggtaatgc gccgtatttc gccaatggcg
     5461 gtggaagagg tgattaagat gcagggatta agtctcaacc cgacatctca ccgagtgatg
     5521 gcgggcgaag agccgctgga gatggggccg acagaattta aactgctgca cttttttatg
     5581 acgcatcctg agcgcgtgta cagccgcgag cagctgttaa accacgtctg gggaactaac
     5641 gtgtatgtgg aagaccgcac ggtcgatgtc cacattcgtc gcctgcgtaa agcactggag
     5701 cccggcgggc atgaccgcat ggtgcagacc gtgcgcggta caggatatcg tttttcaacc
     5761 cgcttttaac gccttgctca tcggacgcag agcagggctt atgatttctt aactggagta
     5821 tcttacgtgc tggaacggct gtcgtggaaa aggctggtgc tggagctgct actttgctgc
     5881 ctcccggctt tcatcctggg tgcatttttt ggttacctgc cctggttttt gctggcatcg
     5941 gtaacaggac tgcttatctg gcatttctgg aatttattgc gcctttcatg gtggctgtgg
     6001 gtggatcgca gtatgacccc gccaccgggg cgtggtagct gggaaccgct actatacggc
     6061 ttacaccaga tgcagctgcg aaataaaaaa cgccgccgtg aactgggcaa tctgattaaa
     6121 cgctttcgta gcggcgcgga gtcgctgccc gacgcggtgg tgctgaccac ggaagagggc
     6181 ggtattttct ggtgtaacgg tctggcgcaa caaattcttg gtttgcgctg gccggaagat
     6241 aacgggcaga acatccttaa cctactgcgt tacccggagt ttacgcaata tctgaaaacg
     6301 cgtgattttt ctcgcccgct caatctggtg ctcaacaccg ggcggcatct ggaaattcgc
     6361 gtcatgcctt atacccacaa acagttgctg atggtggcgc gtgatgtcac gcaaatgcat
     6421 caactggaag gggcgcggcg taactttttt gccaacgtga gccatgagtt acgtacgcca
     6481 ttgaccgtgt tacagggtta cctggagatg atggatgagc agccgctgga aggcgcggta
     6541 cgcgaaaaag cgttgcacac catgcgcgag cagacccagc ggatggaagg gctggtgaag
     6601 cagttgctaa cgctgtcgaa aattgaagcc gcgccgacgc aattgctcaa tgaaaaggtt
     6661 gatgtgccga tgatgctgcg cgttgttgag cgcgaggctc agactctgag tcagaaaaaa
     6721 cagacattta cctttgagat agataacggc ctcaaggtgt ctggcaacga agatcagcta
     6781 cgcagtgcga tttcgaacct ggtctataac gccgtgaatc atacgccgga aggcacgcat
     6841 atcaccgtac gctggcagcg agtgccgcac ggtgccgaat ttagcgttga agataacgga
     6901 ccgggcattg caccggagca tattccgcgc ctgaccgagc gtttttatcg cgttgataaa
     6961 gcgcgttccc ggcaaaccgg cggtagcgga ttagggttag cgatcgtgaa acatgcggtg
     7021 aatcatcacg aaagtcgcct gaatattgag agtacagtag gaaaaggaac acgtttcagt
     7081 tttgttatcc cggaacgttt aattgccaaa aacagcgatt aatccgcctt tgtcatcttt
     7141 tattgccata agccagccga tgctggctta ttttctttgc agtcaaaata cgggcgttag
     7201 attttacaac ggttggtgat tttttgttcg catgattagc catgtctttt tcacggaaat
     7261 agtgttttat actggttggt gatttcttat cgctatatac ctctggtttt tagatccctc
     7321 cttgctttaa aacgttataa gcgtttaaat tgcgcttcag gtgctgtcat actgactgca
     7381 ttaacgcggt aaatcgaaaa actattcttc gccgcgcctg gttgggagta tttcccgcta
     7441 aaattgttta aatataccgc tgtatcatcc ccagggattg gcacaaaaat ttaacgttac
     7501 aacaccacat ccacaggcag tatgatttat gacccatcaa ttaagatcgc gcgatatcat
     7561 cgctctgggc tttatgacat ttgcgttgtt cgtcggcgca ggtaacatta ttttccctcc
     7621 aatggtcggc ttacaggcag gcgaacacgt ctggactgcg gcattcggct tcctcattac
     7681 tgccgttggc ctgccggtat taacggtagt ggcgctggca aaagttggcg gcggtgttga
     7741 cagcctcagc acgccaatcg gtaaagtcgc tggcgtactg ctggcaacgg tttgttacct
     7801 ggcggtgggg ccgcttttcg ctacgccgcg tacagctacc gtttcctttg aagtggggat
     7861 tgcgccgctg acgggtgatt ccgcgctgcc gctgtttatc tacagcctgg tctatttcgc
     7921 tatcgttatt ctggtttcgc tctatccggg caagctgctg gataccgtgg gcaacttcct
     7981 tgcgccgctg aaaattatcg cgctggtcat cctgtctgtt gtcgcgattg tctggccggc
     8041 gggttctatc agcacggcga ctgaggctta tcaaaacgct gcattttcta acggcttcgt
     8101 taacggctat ctgaccatgg atacgctggg cgcaatggtg tttggtatcg ttattgttaa
     8161 cgcggcgcgt tctcgtggcg ttaccgaagc gcgtctgctg acccgttata ccgtctgggc
     8221 tggcctgatg gcgggtgttg gtctgacttt gctgtacctg gcgctgttct gtctgggttc
     8281 agacagcgcg tcgctggtcg atcagtctgc aaacggcgct gctattctgc atgcttacgt
     8341 tcagcacacc tttggcggcg gcggtagctt cctgctggcg gcgttaatct tcatcgcctg
     8401 tctggtcacg gcggttggcc tgacctgtgc ttgtgcagaa ttctttgccc agtacgtacc
     8461 gctctcttat cgtacgctgg tgtttatcct cggcggcttc tcgatggtgg tgtctaacct
     8521 cggcttaagc cagctgatcc agatctccgt accggtgctg accgctattt atccgccgtg
     8581 tatcgcactg gttgtattaa gttttacacg ctcatggtgg cataattcgt cccgcgtgat
     8641 tgctccgccg atgtttatca gcctgctttt tggtattctc gacgggatca aagcatctgc
     8701 attcagcgat atcttaccgt cctgggcgca gcgtttaccg ctggccgaac aaggtctggc
     8761 gtggttaatg ccaacagtgg tgatggtggt tctggccatt atctgggatc gcgcggcagg
     8821 tcgtcaggtg acctccagcg ctcactaaat cactgaacat ttgttttaac cacggggctg
     8881 cgatgccccg tggtttttta ttgtgttgat gggttaggaa ttgatggaaa gtaagaataa
     8941 gctaaagcgt gggctaagta cccgccacat acgctttatg gcactgggtt cagcaattgg
     9001 caccgggctg ttttacggtt cggcagacgc catcaaaatg gccggtccga gcgtgttgtt
     9061 ggcctatatt atcggtggta tcgcggcgta tatcattatg cgtgcgctgg gggaaatgtc
     9121 ggtacataac ccggccgcca gctctttctc gcgttatgcg caggaaaacc ttggcccgct
     9181 ggcaggttac attaccggat ggacctactg ttttgaaatc cttattgtcg ccatcgccga
     9241 tgtgaccgct tttggtatct atatgggtgt cttgttcccg acggtgccgc actggatttg
     9301 ggtactgagc gtggtgctga tcatttgcgc cgtaaacctg atgagcgtga aggtcttcgg
     9361 tgagctggaa ttctggttct cgttctttaa agtcgccacc atcatcatca tgattgtcgc
     9421 cggtttcggc atcatcatct gggggattgg caacggcggg caaccgaccg gtattcataa
     9481 cctgtggagc aacggcggct tcttcagtaa cggctggctt ggcatggtaa tgtcgttgca
     9541 aatggtgatg tttgcttacg gtgggatcga aattatcggg attaccgccg gtgaagcgaa
     9601 agatcctgag aaatcgattc cgcgtgcgat taactccgtg ccgatgcgta ttctggtgtt
     9661 ctacgtcggt acgctgttcg tcattatgtc tatctacccg tggaatcagg ttggcactgc
     9721 cggtagcccg ttcgtactga cgttccagca tatgggcatt acctttgccg ccagcattct
     9781 taactttgtt gtgctgaccg cttcgctgtc ggcaattaac agtgacgtat ttggcgtagg
     9841 ccgtatgctc cacggtatgg cagagcaggg cagcgcaccg aaaattttca gcaaaacgtc
     9901 gcgtcgcggt attccgtggg ttacggtgct ggtgatgact accgcgctgc tgtttgcggt
     9961 gtatctgaac tacatcatgc cggaaaacgt cttcctggtg atcgcttcgc tggcaacctt
    10021 cgccacggta tgggtgtgga ttatgatcct gctgtcgcaa atcgccttcc gtcgccgttt
    10081 gccgccagaa gaagttaagg cgctgaaatt taaagtgccg ggtggggtag caacgaccat
    10141 cggcgggctg attttcctgc tctttattat cgggttgatt ggttatcacc cggatacgcg
    10201 tatctcgctg tatgtcggtt tcgcgtggat tgttgtgctg ttgattggct ggatgtttaa
    10261 acgccgccac gatcgtcagc tggctgaaaa ccaataatac ttgcgttctg taagccggtt
    10321 caggaggaaa tcctgatccg gctattccga aagttatccg cccccgtcct cctcccccaa
    10381 atatccttca gatgatgagt gatcctgcat taggctatgg caaggtgatc agattttcat
    10441 cacaggggaa ttatgatgtt aaatgcatgg cacctgccgg tgcccccatt tgttaaacaa
    10501 agcaaagatc aactgctcat tacactgtgg ctgacgggcg aagacccacc gcagcgcatt
    10561 atgctgcgta cagaacacga taacgaagaa atgtcagtac caatgcataa gcagcgcagt
    10621 cagccgcagc caggcgtcac cgcatggcgt gcggcgattg atctctccag cggacaaccc
    10681 cggcggcgtt acagtttcaa actgctgtgg cacgatcgcc agcgttggtt tacaccgcag
    10741 ggcttcagcc gaatgccgcc ggcacgactg gagcagtttg ccgtcgatgt acctgatatc
    10801 ggcccacaat gggctgcgga tcagattttt tatcagatct tccctgatcg ttttgcgcgt
    10861 agtcttcctc gtgaagctga acaggatcat gtctattacc atcatgcagc cggacaagag
    10921 atcatcttgc gtgactggga tgaaccggtc acggcgcagg cgggcggatc aacgttctat
    10981 ggcggcgatc tggacgggat aagcgaaaaa ctgccgtatc tgaaaaagct tggcgtgaca
    11041 gcgctgtatc tcaatccggt gtttaaagct cccagcgtac ataaatacga taccgaggat
    11101 tatcgccatg tcgatccgca gtttggcggt gatggggcgt tgctgcgttt gcgacacaat
    11161 acgcagcagc tgggaatgcg gctggtgctg gacggcgtgt ttaaccacag tggcgattcc
    11221 catgcctggt ttgacaggca caatcttggc acggggggag cttgtcacaa ctctgaatcg
    11281 ccctggcgcg actggtactc gtttagtgat gatggcacgg cgctcgactg gcttggctat
    11341 gccagcttga cgaagctgga ttatcagtcg gaaagtctgg tgaatgaaat ttatcgcggg
    11401 gaagacagta ttgtccgcca ctggctgaaa gcgccgtgga atatggacgg ctggcggctg
    11461 gatgtggtgc atatgctg
//
LOCUS       DAZSNM010000129        11125 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZSNM010000129 DAZSNM010000000
VERSION     DAZSNM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11125
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.129"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..579
                     /locus_tag="ACHW42_002810"
     CDS             <1..579
                     /locus_tag="ACHW42_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR8944558.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="ACHW42_002811"
     CDS             597..1235
                     /locus_tag="ACHW42_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8944559.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHW42_002812"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHW42_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR8944560.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHW42_002813"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHW42_002813"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR8944561.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="ACHW42_002814"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="ACHW42_002814"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR8944562.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHW42_002815"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHW42_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR8944563.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="ACHW42_002816"
     CDS             complement(5552..6613)
                     /locus_tag="ACHW42_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR8944564.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="ACHW42_002817"
     CDS             complement(6610..8007)
                     /locus_tag="ACHW42_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR8944565.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNASFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="ACHW42_002818"
     CDS             8163..9161
                     /locus_tag="ACHW42_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR8944566.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHW42_002819"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHW42_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11125)
                     /locus_tag="ACHW42_002820"
                     /pseudo
     CDS             complement(10217..11125)
                     /locus_tag="ACHW42_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         2781 a         2982 c         2633 g         2729 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gacgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccg
//
LOCUS       DAZSNM010000130        11120 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZSNM010000130 DAZSNM010000000
VERSION     DAZSNM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11120
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.130"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..209)
                     /locus_tag="ACHW42_002821"
     CDS             complement(<1..209)
                     /locus_tag="ACHW42_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR8944567.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHW42_002822"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHW42_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR8944568.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHW42_002823"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHW42_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR8944569.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHW42_002824"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHW42_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR8944570.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHW42_002825"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHW42_002825"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR8944571.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHW42_002826"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHW42_002826"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR8944572.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHW42_002827"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHW42_002827"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR8944573.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="ACHW42_002828"
     CDS             complement(4598..5320)
                     /locus_tag="ACHW42_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8944574.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHW42_002829"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHW42_002829"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR8944575.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="ACHW42_002830"
     CDS             6520..6738
                     /locus_tag="ACHW42_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR8944576.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHW42_002831"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHW42_002831"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR8944577.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="ACHW42_002832"
     CDS             complement(7472..7672)
                     /locus_tag="ACHW42_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR8944578.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHW42_002833"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHW42_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHW42_002834"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHW42_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR8944579.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..>11120
                     /locus_tag="ACHW42_002835"
     CDS             10101..>11120
                     /locus_tag="ACHW42_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR8944580.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSR"
BASE COUNT         2734 a         3044 c         2667 g         2675 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc
//
LOCUS       DAZSNM010000131        11102 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZSNM010000131 DAZSNM010000000
VERSION     DAZSNM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11102
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.131"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            110..1009
                     /gene="lysO"
                     /locus_tag="ACHW42_002836"
     CDS             110..1009
                     /gene="lysO"
                     /locus_tag="ACHW42_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR8944581.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1153..2805
                     /gene="hcp"
                     /locus_tag="ACHW42_002837"
                     /gene_synonym="priS"
     CDS             1153..2805
                     /gene="hcp"
                     /locus_tag="ACHW42_002837"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR8944582.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            2817..3785
                     /gene="hcr"
                     /locus_tag="ACHW42_002838"
     CDS             2817..3785
                     /gene="hcr"
                     /locus_tag="ACHW42_002838"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR8944583.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3918..5666
                     /gene="poxB"
                     /locus_tag="ACHW42_002839"
     CDS             3918..5666
                     /gene="poxB"
                     /locus_tag="ACHW42_002839"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR8944584.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGT
                     PTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLM
                     GDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITG
                     IRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIIS
                     GRGDEVIELAKTNWLR"
     gene            5703..6704
                     /gene="ltaE"
                     /locus_tag="ACHW42_002840"
     CDS             5703..6704
                     /gene="ltaE"
                     /locus_tag="ACHW42_002840"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR8944585.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6715..8145
                     /locus_tag="ACHW42_002841"
     CDS             6715..8145
                     /locus_tag="ACHW42_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR8944586.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8244..9257
                     /locus_tag="ACHW42_002842"
     CDS             8244..9257
                     /locus_tag="ACHW42_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR8944587.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9254..10084)
                     /gene="amiD"
                     /locus_tag="ACHW42_002843"
     CDS             complement(9254..10084)
                     /gene="amiD"
                     /locus_tag="ACHW42_002843"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR8944588.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10081..10404)
                     /locus_tag="ACHW42_002844"
     CDS             complement(10081..10404)
                     /locus_tag="ACHW42_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR8944589.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(10615..>11102)
                     /locus_tag="ACHW42_002845"
     CDS             complement(10615..>11102)
                     /locus_tag="ACHW42_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR8944590.1"
                     /translation="FINCNFFNVDMIRVNLSKCLFHECSMHGVKIKPWLPVKWTKELI
                     SDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFLEIIFSLKNKYCIKLLSAQDRAF
                     IYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRYRPQSQDGEDFRSHLQYKLPLSA
                     H"
BASE COUNT         2559 a         2930 c         2908 g         2705 t
ORIGIN      
        1 cccctctgat gcataacatt tccttttaat tgcaacttta ctcgcccttc cgctttcagt
       61 agtgtgtcat cgcgcatttc gacttcttct ttgcaaggac tggattttca tgttttctgg
      121 gctgttaatc attctggttc ccctgattgt gggttacctc attccgcttc gccaacaagc
      181 tgcgttaaaa gctattaatc agctattaag ctggatggtt taccttattc tcttttttat
      241 gggtatcagt ctggcgtttc tcgataacct cgccagtaac ctgttggcga ttctgcatta
      301 ttctgccgtc agtattaccg ttattttact gtgtaatatt gccgccctga tgtggctgga
      361 gcgaggcctg ccgtggcgca accaccatca gcaagaaaaa ctcccgtcgc gtattgcgat
      421 ggcgctggag tcgctaaaac tgtgcggcgt agtagtgatt ggttttgcca ttggtctgag
      481 tggactggct ttcttacaac acgcgaccga agccagtgaa tacacgttaa ttttgctact
      541 tttcctcgtt ggtattcagt tgcgcaataa tggcatgacc ttaaagcaga ttgtccttaa
      601 tcgccgggga atgattgtcg ccgtggtggt ggttgtcagt tcattaattg gtggtttaat
      661 taacgccttt attcttgatc tccccatcaa taccgcgctg gcaatggcct ccggtttcgg
      721 ctggtattct ctttccggta ttttattgac cgaatctttt ggtccggtaa tcgggagcgc
      781 ggcgtttttt aatgatctgg cccgtgaact gattgctatt atgttgatcc ctgggctgat
      841 tcgccgcagc cgctctactg cactgggctt atgcggtgcc acatcaatgg atttcaccct
      901 gcccgttctt caacgtactg gcgggctgga tatggtcccg gcggcaattg ttcacggttt
      961 tattcttagc ctgttagtgc cgatcctcat cgcctttttc tctgcgtaat acctctctgg
     1021 cggtagttcc ctgccgccaa aattgcgcta aatcaatctc ccttaaagtt gcatgaaaaa
     1081 tcccttttat ccccgcgtta agcgtcttac ccttaaacat gtatattaaa tataacttta
     1141 aaaggtgtga tcatgttttg tgtgcaatgt gaacaaacta tccgtactcc ggcagaaaac
     1201 ggctgctcat acgcgcaggg gatgtgtggt aaaacggcgg aaacttctga ccttcaggat
     1261 ttactcatcg cggcgctgca agggctttct gcctgggcgg taaaagcgcg tgaatacggc
     1321 atcatcaacc acgatgttga cagcttcgcg ccacgtgcct ttttctcaac cctgaccaac
     1381 gttaacttcg attctccgcg tattgtcggc tacgctcgtg aagcgattgc cttgcgcgag
     1441 gcgctgaaag cacaatgcct ggctgtagat gccaacgcgc gcgtcgataa cccgatggct
     1501 gacctgcaac tggtgagcga tgatctcggc gagctgcaac gtcaggcagc agaatttacc
     1561 cctaacaaag ataaagcggc gattggcgaa aacattctcg gcctgcgtct gctgtgcctg
     1621 tatggcctga aaggtgcggc ggcctatatg gaacacgcgc atgttctcgg tcaatacgac
     1681 aacgatattt atgcccagta ccataaaatc atggcgtggc tggggacctg gcctgccgat
     1741 atgaacgcgc ttcttgagtg ttcaatggaa atcggccaga tgaacttcaa agtgatgagc
     1801 attctggatg caggcgaaac cggtaaatac ggtcacccga cgccaaccca ggtcaacgtc
     1861 aaagcgacgg cgggtaaatg cattctgatt tccggtcacg atctcaaaga tctctacaac
     1921 ctgctggaac agaccgaagg cacgggcgtt aatgtctaca cccacggcga aatgctgcct
     1981 gcgcatggct acccggagct gcgtaaattc aagcatctgg tcggtaacta cggcagcggc
     2041 tggcagaatc agcaagtgga gttcgctcgt ttccctggcc ccatcgtgat gacctcgaac
     2101 tgcatcatcg acccaaccgt aggcgcttat gacgatcgta tctggacccg cagcatcgtt
     2161 ggctggcctg gcgtgcgtca tctggatggc gaagatttct ctgcggtcat cgcccaggcg
     2221 caacaaatgg cgggcttccc gtacagcgaa attcctcacc tgatcaccgt gggctttggt
     2281 cgccagacgc tgcttggtgc agccgatacg ctgattgatc tggttagccg tgaaaaactg
     2341 cgtcatatct tcctgcttgg tggctgtgac ggcgcacgcg gcgagcgcca ctacttcacc
     2401 gatttcgcca ccagcgtgcc ggatgactgc ctgatcctga ccctcgcctg tggtaaatat
     2461 cgctttaaca aacttgagtt tggcgatatc gaaggtctgc cgcgtctggt agatgcaggt
     2521 caatgtaacg atgcttactc agcaattatt ctggctgtca ctctggcaga gaaactgggc
     2581 tgtggtgtga acgatctgcc gctgtcgctg gtgctctcct ggtttgaaca gaaagcgatt
     2641 gtcattctgc tgacgctgct ttctctgggc gtgaaaaata tcgtcaccgg cccgactgca
     2701 cctggtttcc tgacaccaga cctgctggcg gtgctgaacg agaaattcgg cctgcgttct
     2761 atcaccactg ttgaagaaga catgaagcaa ctgttgaacg cgtaaggagt ttgtcgatga
     2821 cgatgccaac gaatcaatgc ccgtggcgga tacaggttca tcacattacg caagaaacgc
     2881 cggatgtgtg gacgatttcc ctgatttgcc acgatcacta cccatatcgc gccgggcaat
     2941 atgcactggt cagcgtgcga aactcagcgg aaacgctgcg tgcttacacc atttcctcca
     3001 cgccaggcgt gagtgaatat atcaccctga ccgtgcggcg gattgatgac ggtgtcggct
     3061 cccagtggct gacgcgcgat gtaaaacgcg gtgattatct ctggctttcg gacgcgatgg
     3121 gggaatttac ctgcgacgat aaagcagaag ataaattcct gttgctggcg gcaggctgcg
     3181 gcgtcacgcc gattatgtcg atgcgtcgct ggcttgcgaa gaaccgtcca caggccgatg
     3241 tgcgggtgat ctacaacgtg cgtacgccgc aggatgttat tttcgccgat gagtggcgta
     3301 actatccggt aacgctggtg gcagaaaata acgttaccga aggctttatc gctggtcgcc
     3361 tcactcgcga actgctggca gatgtacctg acttagcttc acgtaccgtg atgacctgcg
     3421 gcccggctcc gtatatggat tgggtagagc aggaagtgaa agcgctcggc gtgacgcgtt
     3481 tctttaaaga gaaattcttc accccagtag cagaagcggc gaccagcggt ctgaaattca
     3541 ccaaactgca accggcacga gaattttacg ccccggttgg caccacgcta ctggaggcgc
     3601 tggaaagcaa taacgttccg gttgtcgctg cctgccgtgc gggtgtttgc ggctgctgta
     3661 agacaaaagt ggtttccggt gaatatacgg tgagcagtac aatgacgctg accgacgccg
     3721 aaatcgctga aggttacgta ctggcctgct cctgccatcc gcagggggat ttggttctcg
     3781 cataatcgcc ttatgcccga tgatattcct ttcatcgggc tatttaaccg ttagtgcctc
     3841 ctttctctcc catcccttcc ccctccgtca gatgaactaa acttgttacc gttatcacat
     3901 tcaggagatg gagaaccatg aaacaaacgg ttgcagctta tatcgccaaa acactcgaat
     3961 cggcaggcgt gaaacgcatc tggggagtca cgggcgactc tctgaacggt cttagcgaca
     4021 gtcttaatcg catgggcacc atcgagtgga tgtccacccg ccatgaagaa gtggcggcgt
     4081 ttgccgctgg cgctgaagca caactttcag gagaactggc ggtctgtgcc ggatcgtgcg
     4141 gcccaggcaa cctgcacttg atcaacggcc tattcgattg ccaccgcaat aacgttccgg
     4201 tactggcgat tgccgctcat attccctcca gcgaaattgg cagcggctat ttccaggaaa
     4261 cccacccaca agagctattc cgcgaatgta gtcactattg cgagctggtt tccagcccgg
     4321 agcagatccc acaagtgctg gcaattgcta tgcgcaaagc ggtgcttaac cgtggcgttt
     4381 ccgttgttgt attaccgggc gacgtggcgt taaaacctgc gccagaaggg gcaaccacgc
     4441 actggtatca tgcgccacag ccagtcgtga tgccggaaga agaagagtta cgcaaactgg
     4501 cgcaactgct gcgttattcc agcaatatcg ccctgatgtg tggcagcggc tgcgcggggg
     4561 cgcataaaga gttagttgag tttgccggga aaattaaagc acctattgtt catgccctac
     4621 gcggtaaaga gcatgtcgaa tacgataatc cgtatgatgt tggaatgacc gggttaatcg
     4681 gattctcgtc aggtttccat accatgatga acgccgacac gttagtgcta ctcggtactc
     4741 agttccccta ccgtgcgttc tacccgaccg atgccaaaat tattcagatt gatattaatc
     4801 cagccagcat cggcgcgcat agcaaggtag atatggcgct ggtcggcgat atcaaatcaa
     4861 ccctgcgcgc attgctgcca ctggtggaag aaaaaaccga tcgcaagttt ctggataaag
     4921 cgctggaaga ttaccgcgat gcccgcaaag ggctggaaga ttaccgcgat gcccgcaaag
     4981 ggctggacga tttagctaaa ccgagcgaga aagccattca cccgcaatat ctggcgcagc
     5041 aaattagtca ttttgccgcc gatgacgcca tctttacctg tgacgtcggc acgccaacgg
     5101 tatgggcggc acgttatctg aaaatgaacg gcaagcgtcg cctgttaggt tcgtttaacc
     5161 acggttcgat ggctaacgcc atgccgcagg cgttgggtgc gcaggcaacc gagccggaac
     5221 gtcaggtggt cgccatgtgc ggcgatggcg gttttagcat gttgatgggc gatttcctct
     5281 cagtagtgca gatgaaactg ccggtgaaaa ttatcgtctt taataacagc gtgctgggct
     5341 ttgtggcgat ggagatgaag gccggaggtt acctgacaga cggtactgag ctgcacgaca
     5401 ccaactttgc ccgcattgcc gaagcgtgcg gcattacggg tatccgtgta gaaaaagcct
     5461 ctgaagttga tgaagccctg caacgcgcct tctctatcga cggtccggtg ttggtggatg
     5521 tggtggtcgc caaagaagaa ttagccattc caccgcagat caaactcgaa caggccaaag
     5581 gttttagcct gtatatgttg cgcgcaatta tcagcggacg cggcgatgaa gtgatcgaac
     5641 tggcgaaaac gaactggcta aggtaaaaag ggtggcattt cccgtcataa taaggacatg
     5701 ccatgattga tttacgcagt gataccgtta cccgaccgag ccgcgccatg ctcgaagcga
     5761 tgatggccgc cccggttggg gacgacgttt acggagacga ccctaccgtt aatgctctgc
     5821 aggactatgc agcagagctt tccggtaaag aagccgccat ttttctgcct accggcactc
     5881 aggccaacct ggtcgctctg ctcagtcact gcgaacgtgg cgaagagtat attgtcggtc
     5941 aggccgcgca taactatctg tttgaagccg gtggcgcagc ggtgctgggc agtattcagc
     6001 cgcaacccat cgacgcggct gccgacggca cgctaccgct ggataaagtg gcgatgaaaa
     6061 tcaaacccga cgatatccat ttcgcccgca ccaaattact cagtctggaa aacacccaca
     6121 acggcaaagt gctgccgcgt gaatacctga aagaagcatg ggaatttacc cgcgagcgca
     6181 atctggcgct gcatgttgac ggtgcgcgca tctttaatgc cgtggtggct tacggctgcg
     6241 aactgaaaga gatcacgcaa tattgtgatt cgttcaccat ttgcctgtcg aaaggtcttg
     6301 ggacgccagt cggttcatta ctcgtcggta atcgtgatta cattaaacgt gccatccgct
     6361 ggcggaaaat ggccggtggc gggatgcgtc agtccgggat tctggctgcc gccgggatgt
     6421 atgccctgga aaataacgtc gcacggttgc aggaagatca cgacaacgcc gcctggatgg
     6481 cggagcaact gcgtgaagca ggcgcggatg tgatgcgtca ggacactaat atgctgtttg
     6541 ttcgcgtcgg ggaagaaaat gctgccgcgt taggcgaata catgaaagcg agaaacgtgc
     6601 tgattaacgc ctcgccgatt gtccgcctgg tgacgcatct tgacgtctcg cgcgaacaac
     6661 tggcggaagt cgccgcccac tggcgcgcat tcctggcgcg ttaaggagag agacgtgccg
     6721 caacgcattt tagttctcgg tgccagtggc tacattggtc agcatctggt gcgcacactc
     6781 agccagcaag ggcatcagat cctagcggcg gcacgtcatg tcgacaggct tgcaaagctg
     6841 caactggcaa acgtcagttg ccataaagtc gatctcagct ggccggataa ccttccggcc
     6901 ctgttgcagg atatcgatac cgtctatttt ctggtgcaca gcatgggcga aggcggcgat
     6961 tttatcgctc aggagcgcca gctggctctc aacgtccgcg atgcgctacg tgaagtacca
     7021 gttaagcaat taatctttct cagttcgttg caggccccgc cacatgagca gtcggatcat
     7081 ctgcgagctc gtcaggctac ggcggacatt cttcgtgaag cgaatgtacc agtgaccgaa
     7141 cttcgggccg gaattatcgt tggcgcaggt tcagcggcgt tcgaagtcat gcgcgatatg
     7201 gtctacaacc tgccagtgtt aacgccgcca cgctgggtac gttcacgcac cacgcccatc
     7261 gcgctggaaa acttgctgca ctatctggtg gcgctgttag accatccagc caacgaacac
     7321 cgcatcttcg aagccgccgg accagaggtg ctcagttatc agcaacagtt tgaacatttt
     7381 atggcggtga gcggtaagcg ccgctggttg atccccatcc ccctccccac ccgctggatt
     7441 tcggtgtggt ttctcaatgt gattacttcc gtaccgccca ccaccgccag ggcgttgatt
     7501 caggggctga aacacgatct gctggcagat gataccgcgc tacgtgcact catcccacaa
     7561 cggctgatcg ctttcgatga cgcggtacgt agcacgttga aagaggaaga aaaactggtc
     7621 aactccagcg actggggcta cgacgctcag gcctttgccc gctggcgacc ggagtacggt
     7681 tatttcgcca aacaggcggg gtttaccgtt aaaacgtccg ccagccttgc ggctttatgg
     7741 ctggtggtga accaaatcgg cggtaaagag cgttatttct ttggcaatat tttgtggcag
     7801 acacgggcgt tgatggaccg cgcgatcggt cataatctgg cgaaaggccg cccggagcgc
     7861 gaatatttac agaccggcga tgcggtggat agctggaaag tgattgtcgt tgaaccgaaa
     7921 aagcaactta cgttgttatt tggcatgaaa gcgccggggc tgggacgact gtgttttacc
     7981 ctggaagata aaggtgacta tcgtactatc gatgtccgcg ctttctggca tccgcacggt
     8041 atgccggggc tgttttactg gttattgatg atccccgcgc atctgtttat ttttcgcgga
     8101 atggcaaaac aaatcgccag actggcagaa caaagcacag attaatcgac aaaacctgcg
     8161 ttattctttc atgtttccca ttgcatcatg ggggaaatca cggaagaatg cgcacgtcat
     8221 tttctcaaca acagtggatt gatatgaagg tactggttac cggcgccacc agcggcttag
     8281 gtcgaaacgc ggtagagttt ttatgccaga aaggcatcag cgtgcgtgcg agcggtcgca
     8341 acgaagcaat gggtaaattg ctggaaaaaa tgggcgcaga gtttgttccg acggatctga
     8401 ccgagctggt ttcatcacaa gctaaagtga tgctcgcggg cattgatacg ctgtggcact
     8461 gctccagctt tacctcaccc tgggggacac aacaggcttt cgatctggct aacgttcgcg
     8521 ccactcgccg cctgggtgaa tgggctgtcg cctggggtgt gcgtaacttt attcatatct
     8581 cttccccctc cctgtacttc gattatcacc accatcgcaa tattaaagaa gattttcgcc
     8641 ctcaccgctt cgccaacgag tttgcccgca gcaaagcagc cagcgaagaa gtgatcaata
     8701 tgctttcgca ggcgaatcca caaacgcgct ttactattct gcgcccacaa agtctgttcg
     8761 gaccgcacga taaagtcttt attccccgtc tggcgcatat gatgcaccac tacggcagta
     8821 ttctgttacc gcatggcggc agtgcgctgg tcgatatgac ctactatgaa aatgccgtgc
     8881 acgcaatgtg gctggcaagc caggaagcct gcgataagct accttccggg cgtgtgtaca
     8941 acatcaccaa cggcgagcat cgcacactgc gcagcatcgt gcagaagctg atcgacgagt
     9001 tgaatattga ctgtcgtatt cgttccgtcc cttacccgat gctggatatg atcgcccgca
     9061 gcatggagcg tttaggccgc aagtcagcaa aagagccgcc gctgacccac tacggcgcct
     9121 ccaagttgaa ttttgacttt acgctggata ttacgcgtgc gcaggaagag ttaggttatc
     9181 agccggtcat caccctggat gaaggcatcg agaaaactgc cgcctggcta cgcgaccacg
     9241 gaaaactgcc gcgctaatcc tgcccgtatt tctccagcaa tgcttcggca atcgcctgag
     9301 tttctgcatc cgcttcgccg ttatataacg tcgggcggaa atgcatctgg aatgccataa
     9361 tcacgcgccg ctgctcgcgc ggtgtcatat caggttttac gtcataaccg tagcgcgcca
     9421 aaagctccag caatgacgcg gtatctaccg gtgtgtgcgg cgcgcgcccg gcaaggtaaa
     9481 agttcacccg ctgcgcatcc ggccaggcac caatcccctg ctgcgccagt tgctgccagg
     9541 gaaataatgg ccccggatcg tctttgcgct gcggtgcgat atccgcatgt gccactacgt
     9601 tttccggctt gatgtgataa cgggcaataa tatctttcgc cagcggaata agcgcctgaa
     9661 tctgtgcctg ttcaaacggg gcaaaatatt tcactccgga tgatttttgc cagccacggt
     9721 tttccagctc aatgccaata gaggtgtcgt taaggcgcgt tgccccgcgc caggcgctaa
     9781 tccccgcatg ccaggccagt tcttgttccg gcaccagttg ccagatgcgc ggtttaccgt
     9841 tgtatcgcgg tggcaccgca gggatcagat aatgcgagct gacctgctta tcagtcagtg
     9901 tcgccagcga gctatcaaaa tcatctgcgg tgtagtggat caccagcact ttaatgcgcg
     9961 gatacgccgc ctgcgcctgg tgtcgggtat caagctgata tccctctttc tcgacaatgc
    10021 ctttttcgcc tgcacaccct gccagtaaca gagcggcagc gaccagccag aagactcttc
    10081 tcatcgacgc gttttcaccg ccgtaccgct gacgctaacc atcagcatac tgccgttttg
    10141 cccgaccgtt tcgtagtcga tatcaatacc aactacggca tcggccccca gcgcccgcgc
    10201 ctgggagcct aattcctcaa aggcgatctc ccgtgcttta cgcagttctt tttcatacgc
    10261 accggaacgt ccgccaacga tatcgcggat accggcaaag aaatcacgga aaatattggc
    10321 acctaaaatc gcttcgccgg tcaccacacc gcaatattca acgatggtca ggccttccag
    10381 agttggggtt gtagaaaatt gcatgatcga tctccttgtt tgttaagcct tcgatctcta
    10441 aagcattatc ggactgatat gccatgttgg gaagaaggtt ttgatcagtt taggaaatca
    10501 tctcattgtg tcaaaaacac ccacttaagt ctttacgatt atccgttgca taacccgata
    10561 ttggtttcat tatagggtac cgcttacctg tcttattatt ttgcaccaac aacattaatg
    10621 agcggaaaga ggcaacttat attgtaaatg actcctaaag tcttccccat cttgtgactg
    10681 aggtctatac cgctggattg ctttttggtc agccgccgca aaacaagaaa gtaataagat
    10741 ctcaatgctt ttgtcattaa tataagcgaa gatcattctt gcaaactcgt atataaacgc
    10801 ccgatcctga gcgcttaata gtttaataca atatttattt ttaagagaga aaataatttc
    10861 aaggaaagca tccgcagcaa ccttcacgtt attcccggcg ttcatattat atatatccct
    10921 tgcacagata gagtacagag atagtaaaca gccatacaaa taatcactta ttaattcttt
    10981 tgtccatttt acaggcaacc agggttttat cttgacacca tgcatactac attcatgaaa
    11041 aagacatttg ctgagattaa ctcttatcat atcaacattg aaaaaattgc agttaataaa
    11101 tt
//
LOCUS       DAZSNM010000132        11016 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZSNM010000132 DAZSNM010000000
VERSION     DAZSNM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.132"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..594
                     /gene="ais"
                     /locus_tag="ACHW42_002846"
     CDS             <1..594
                     /gene="ais"
                     /locus_tag="ACHW42_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR8944591.1"
                     /translation="FCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNKTLA
                     RLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFDLYS
                     SNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNHCLT
                     YIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(633..1058)
                     /gene="nudI"
                     /locus_tag="ACHW42_002847"
     CDS             complement(633..1058)
                     /gene="nudI"
                     /locus_tag="ACHW42_002847"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR8944592.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1337..1879
                     /locus_tag="ACHW42_002848"
     CDS             1337..1879
                     /locus_tag="ACHW42_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR8944593.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            1966..3168
                     /locus_tag="ACHW42_002849"
     CDS             1966..3168
                     /locus_tag="ACHW42_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR8944594.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3232..3693
                     /locus_tag="ACHW42_002850"
                     /pseudo
     CDS             3232..3693
                     /locus_tag="ACHW42_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3734..4684)
                     /locus_tag="ACHW42_002851"
     CDS             complement(3734..4684)
                     /locus_tag="ACHW42_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR8944595.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(4877..6067)
                     /gene="glpC"
                     /locus_tag="ACHW42_002852"
     CDS             complement(4877..6067)
                     /gene="glpC"
                     /locus_tag="ACHW42_002852"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR8944596.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6064..7323)
                     /gene="glpB"
                     /locus_tag="ACHW42_002853"
     CDS             complement(6064..7323)
                     /gene="glpB"
                     /locus_tag="ACHW42_002853"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR8944597.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7313..8941)
                     /gene="glpA"
                     /locus_tag="ACHW42_002854"
     CDS             complement(7313..8941)
                     /gene="glpA"
                     /locus_tag="ACHW42_002854"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR8944598.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9214..10572
                     /gene="glpT"
                     /locus_tag="ACHW42_002855"
     CDS             9214..10572
                     /gene="glpT"
                     /locus_tag="ACHW42_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR8944599.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            10577..>11016
                     /gene="glpQ"
                     /locus_tag="ACHW42_002856"
     CDS             10577..>11016
                     /gene="glpQ"
                     /locus_tag="ACHW42_002856"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR8944600.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHT"
BASE COUNT         2519 a         2750 c         2972 g         2775 t
ORIGIN      
        1 ttttgccgct catcattgaa gtcaaaaaaa tatttcatca ttttactggc gctcgctgca
       61 attgccggac tgggtactca tgccgcctgg agtagcaatg gtttgccacg tatcgacaat
      121 aaaacattgg ccagactggc acagcagcac ccggttgtcg ttttgtttcg ccatgctgaa
      181 cgttgcgatc gttcaaccaa tcaatgcttg tcagataaaa caggtattac ggttaaaggt
      241 acccaggatg cccgtgaact gggcaacgct tttagcgctg acatctctga tttcgatctt
      301 tattccagta ataccgtccg gaccattcag tcggcgacct ggttttccgc gggtaaaaag
      361 ctgacggtag ataaacgact tcttcagtgc ggtaatgaga tttatagtgc aattaaggac
      421 ttacaaagca aagcgcctga taaaaatatc gttattttca cccataatca ttgcctgaca
      481 tatattgcta aaaataagcg tgatgcgata tttaaacctg attatctgga tggtttagtc
      541 atgcatgtgg aaaaaggcaa agtttatctg gatggggaat tcgttaacca ctaaattttc
      601 aatctgacag ccagtgatgg ctgtcatcat tgttaaagaa gacctttcaa acgtaacgtt
      661 tttcgggtgg caacatttaa atcataatgc accagatctt caggttttac ccacgcgtag
      721 tcctgaaact cttcgtttat tttcacttct cggttggcag aaacgcagtc aaaaatcagg
      781 taaatcatat aaatctcttc cttgcgacca tctgcatatg tcttggtgcg aatatcatcg
      841 ctgaaggtcc acggcgtgat ttctgtcaaa agcagctgtt ctcccagttc ttcgcgaatt
      901 tcgcggcgta gtgcctcttc aattcgttcg ccagactcca cgccgccacc cgaaagcgcc
      961 cattgaccgg ggaaaacgcc gcgatcgtcg gccattttgc acagcaaata agcaccatca
     1021 ttttgaatca aagggcatac aatagtccgt tgtcgcacag gggtattcct tataaataaa
     1081 aagaaaccat gagaatacgg taactcagaa atatgtgaaa gtgacagtaa tctcttttgc
     1141 aatgataact gcacttatta ttatttttgt gtgtatcggt aaagtgtgtt tattttttcg
     1201 gcgtttaatt atttaagaag tatttagtta caataagtga ctagatccct tttttcgctc
     1261 attgaatcct ctgtgccagg cagtacaatc ccgcagcttt gggaattttg attaattgct
     1321 tgtgggaaag gggataatga aaaaaattgc gctagcaggt ctggccggaa tgctgttggt
     1381 ttctgcatcg gtcaatgcaa tgagcatcag cggccaggcg ggtaaagaat acaccaatat
     1441 tggtgtcggt tttgggactg aatcgacggg cctggcttta agcggtaact ggacacataa
     1501 cgacgacgac ggtgacgtcg cgggcgtggg gctggggttg aatctgcctc tcgggccgtt
     1561 aatggcgacc gttggcggaa aaggcgtgta caccaacccg aattacggcg atgaaggtta
     1621 tgccgcagcg gtaggaggtg gtttgcagtg gaaaattggc aacagcttcc gtttgtttgg
     1681 cgagtattac tactctccgg attcgctctc cagcggtatt caaagttatg aggaagcgaa
     1741 tgctggcgcg cgttacacca ttatgcgtcc agtcagtatt gaggcgggtt atcgctacct
     1801 gaatctgtcg ggtaaagacg gtaaccgcga caacgctgtg gctgacggcc tgtatgttgg
     1861 ggttaacgcc agtttctgat cccttccggc gcggcattca gtttcttact acgccttatc
     1921 tccctttgcg ttatagtgat tcgataacaa aagcaggaga taaccatgtt aaaagtggaa
     1981 atgctatcca ccggggatga agtgttatac gggcaaatcg ttgacactaa cgctgcctgg
     2041 ctggccgatt ttttctttca tcaggggctg ccattatctc gccgcaatac ggtgggggat
     2101 aaccttgatg acttagtcac cattcttcgc gaacgtagtc agcacgccga tgtgctgatc
     2161 gttaacggcg ggctgggacc gaccagcgat gatttaagcg cactcgccgc tgcgacagca
     2221 aaaggtgaag gcctggtgct gcatgaagcc tggctcaaag agatggaacg ctatttccac
     2281 gaacgtggac gagtaatggc accgagcaac cgtaaacaag cggagctgcc tgccagtgct
     2341 gaatttatca ataacccggt aggcaccgcc tgtggttttg ccgtgcagct taatcgttgc
     2401 ctgatgttct ttactcccgg cgtaccgtca gaatttaagg tgatggtcga gcacgaaatc
     2461 ctgccgcgcc tgcgcgagcg tttttcttta ccacagccgc cggtttgtct gcgtttaact
     2521 acctttggtc gttcggaaag cgatctggca caaagcctgg acactctaca actgccgccg
     2581 ggcgtaacaa tgggctatcg ctcctcaatg ccgatcatcg aactgaaact caccggaccg
     2641 gcaagcgagc aacaggcgat ggaaaaactg tggctggacg ttaaacgtgt tgccggacag
     2701 agcgtgattt tcgaaggcac cgaaggactg cccgcgcaga tcagtcgcga attgcaaaac
     2761 cgccagttca gcctgacgtt gagcgagcaa ttcaccggtg gtttattggc tttgcaactt
     2821 tctcgcgcag gtgctccatt gctggcgtgt gaagtgattc cttcacagga agaaaccctg
     2881 gcgcaaactg cgcactggat tacagaacgg cgggccaacc attttgccgg gctggcactg
     2941 gctgtttcgg gtttcgagaa cgagcatctc aactttgcgc tagccacgcc agacggcact
     3001 ttcgctctgc gtgtgcgttt cagcactaca cgctacagcc tggctatccg tcaggaagtg
     3061 tgcgcaatga tggcactgaa tatgctacgc cgttggttaa acggccagga catcgctagt
     3121 gagcatggct ggattgaggt tgttgagtcc atgactttat ccgtctgatt tcgccactct
     3181 gtgccggttt actgcaccgg cacattcccc ctccggcaaa atttgtccaa ccctccctac
     3241 ggtgagcgtg gtgttggggc cagtgtggca cgggctgcgc gctggggacg cattgagaat
     3301 tacatggcgc aagtcaacga ttcgctttgt ctgttagtgc aggtggaaag taaaacggca
     3361 ctggataacc tggacgagat cctcgacgtc gaagggattg atggcgtgtt tattggacca
     3421 gcggatcttt ctgcgtcgtt gggctacccg gataacgccg ggcacccgga agtgcagcga
     3481 attattgaaa ccagcattcg gcggatccgt gctgcgggta aagcggctgg ttttctggct
     3541 gtggctcctg atatggcgca gcaatgcctg gcgtggggag cgaactttgt cgctgttggc
     3601 gttgacacga tgctctacag cgatgccctg gatcaacgac tggcgatgtt taaatcaggc
     3661 aaaaatgggc cacgcataaa aggtagttat tgatatcaaa ggcccatggg gatcggctgt
     3721 gggcctgtgt taattagtgg ttattcgctg ccagatcggc ttcgcttagc tgggtggccg
     3781 cgagcacttg gtcacgatca atgccctgtt ccagcatgcg caaagcaatt ttaatggctt
     3841 gttcatgcat accttcctgc aaaccttcta atttaccttg ctgcaaacct actaatttac
     3901 cttcttgcca gccaatttga tgtccttcct gccgtagccg ttcagcaata gtcattaatc
     3961 tctccttttg tagtggtgaa cgttcggcaa tctcctgaat aaaacgggtg aaacgggagg
     4021 tatcgccgca ttgcagcaga taattaaaca gtgtttgtag ctggctgtca ttagtgaagc
     4081 ctctaaccaa aagcgtggtg atcctgtcga ccatgccgat taaatcgtgg tcgcgaatat
     4141 gcttttgaat cagttccagc agagctatac gccgatgttg catgatctca tcgtcaggta
     4201 cgatggtgat atccaccaac ggaaaagctt cggtgtacaa ctgccgggca agttgcggat
     4261 cgtcaaactc atccagccag ttaagtgagt acgggtaggg cgatgtttcg ccatgataaa
     4321 acagcaatgg caccaccagc ggaactctgt catagccttt atctaggtga ctctgcatgg
     4381 cggcagtggc atagcgcatt ggccgaaaag ccatattctt ttctgcagag ctttgatgtt
     4441 caatcacgca gtagatatag ccgtcaccgt cgctggtttc cacggaccac aaaacatccg
     4501 agtagtaagc gcgtaaactt ttttcaataa aactagtggg ttccaggcgt aaggtttgca
     4561 ggttgcaaag cttgcgcagt ggttctggta aatgtatttc gagaaaatcc cgtgcggttt
     4621 cgggtgtgaa tataaaggtt ttaaataccg catcatgcgg ggaggaggtt gttgattcgg
     4681 tcatggttgt ccgtcacctg atatttatga tgacgcgaca ttacggttat cccaggcctt
     4741 tgacatctgc cagtttcagc ctttgcgaga cgttttcaga ggaattttca ctgcgttgca
     4801 acgcatacat tgggcacgga atcgtgtacg caaaaatgcc cggtaaccac atgcttaccg
     4861 ggcatcagaa gggagtttaa gccagcgcct gggccagtag cgtaatcgga tgttcgcagc
     4921 gaagacttgt ggacatctct atctgccatt tacaggtttc gcagtcggtg accaccagat
     4981 ctgcgccgct ttcttctatc tggcggaaca gtggtgcgcc gatggcttgt gaggtggggt
     5041 agttctcttt tttgaaaccg taagtacccg caataccgca gcactgggaa tccagcaccg
     5101 ttaactcaag ccccgggatg ttacgcaaca gctccagggt gtagagcgtc cagcccattt
     5161 tttccatatg gcacggagtg tgataaacca ctttcagcgg cagcggtttc agcggtaacg
     5221 ttttgccttc gtccagcttg cgccacagcc agcgggttgc cagttcgata tgatcgcgca
     5281 agcctttgtt gtcgacattc agcacttccg ggtattcgtc gcgcagggca aatgtacagg
     5341 ttgaggaggt ggcaatcacc ggaatgcctt ttactcccac agcttcgcgg atcgactcta
     5401 cattcgtaat tgcctgtttg cgtgctttat cggtaaagcc gttggcgatt agcggtacgc
     5461 cgcagcattt ttctttgctg agcagttgta caccggtacc cattgcgttg agcactttaa
     5521 ttaaatcttt acctaactgc ggatggttgt agttaacgaa gcagccgtga aagaaagcga
     5581 cctggtcttt atattgtgct tgctgagccg ctatgctgcg ataccagcga cggaacgtgc
     5641 cgaaggagta tttcggtagc gtgcgacgat gatcgatttt taacgccgca tcaagcagct
     5701 gccgcaccgg tttcagcgag gtagcggtgt tgacgattgg tgcgaacggc gtggaaacgc
     5761 tacccatcag gtcggtatga ctcaacacaa aattacgcag cgacgggcgc gtggtgtcat
     5821 atttcgcccg cgcgcgctgg ataatatcgc caatcttcac atcggacggg caggcgactt
     5881 cacaacgttt gcagttgatg caatatttca gcgcctcgtc atacagtgcg ccatctttca
     5941 aacgcagacg ctcgccatcc ggcccggctt gttttggccc tggataaccg ggattcaccc
     6001 ggctcaccgg gcaggcggtg gtgcagacgg tgcacttaat gcagttttcg aagctggtgt
     6061 cgttcattgt tggcctcctg cgcgttgggc aatctgttga gcggcatgta aagcactgac
     6121 ggcacaaaca ccgccgccgc atccctgggc gatgggatca aatccgccca gcaccgaacc
     6181 gatggcaaac aggttctcaa tggtttgccc tgcctgcgac gggcgtagcg tctcatcagt
     6241 ggttacaccg aactgctgcc acggttgcgg cgcaaaaaaa tcgcccttat accattcacc
     6301 ccgcgtggcg gtttgtagca catcaaggcc gagaatcggc tcgcgaatgc cgttacgttc
     6361 cgctactagt ccgccactaa agaaactgcc gctggcgaga accgcgaaac gtggacgtag
     6421 cggaatatcg gcgtgattgc gggtccagat ttcattcact acgccatttt tacaggtcac
     6481 ttttttcact tcatcgcccg gcatccacac tccaccttgg cgcacaaact ggcgctgtaa
     6541 ctggttttgc agacgaatgc ccagcacgga aggcggcagc gttggcaaaa gcatcagtga
     6601 acaaggtagt ttttcattca accaacgcca cagtttgtca tcggccaggc cgaagcaggc
     6661 gggcatcagg atcatttcgc aggtattggc gacaggaata agcgcatcaa gtaacagcgg
     6721 ccagttttct tcattatcaa ggaaacgggc gatattcacc gcgcgaaatt cggtggcgtt
     6781 attgcgcagg acatccagtt ccggcagctc tatttctgcg gtttcaaccg aaagatccag
     6841 ttcgcgcaac gaagccgccg caagatgcgc ctggaaatcc atcagcccgc ttatccccac
     6901 tacacatatc ttcttcgcgg gtagcggcca gacgggaact tctggcgaac ttaaccaggt
     6961 tgcacgcaaa gtgccgagcg gcgtaatccg ctggtgagcc agttctacgc tgccctgcaa
     7021 ttgcgcaccg ctttcagcga ttaatgcctg cgcctggcaa gcgagatcga gcacgcgttg
     7081 tggcccgaga agagagtaag gatgtgcagg tgcctgctga cgcaaagatt ccagtccact
     7141 gtgaatgtct gccacgggtt gaccatctgg cagatggctc agcaaatcca gcgaaccgga
     7201 tgagaaatgc aacgcgcttt gaccacgcgt gacaatggca cagcgcaggc cgtgtttttg
     7261 cagttgcagg ccacagagta atccggcgag gccgccgccc ataatgacag tatcaaagcg
     7321 catctttctg ctccttctcc agaccacaca gtccctgata aacccagcgg gtaaattcgc
     7381 tttcgcgcag tgcgtctccc caggcgatgg gttgcacgcc tttccagcgt tcgttaagga
     7441 aggtggaaag ttgctcgata gattgcgcgg acgtcgtgac gttaaaccgt tgcagcagtc
     7501 cggcagcgcg gcaggcgcag agttcgccct ggcaggtgcc catccccaca cgggtacgac
     7561 ggcgtaaatc cagcaggcta ttaacgttta aattttctac cgcgtactgc acttcacccg
     7621 cagttaccgc ttcgcactca cataccaggc tacggtgtag acggccttcg ctcagccagg
     7681 caggcgtgcg atcgccatga cgataaaccg cagaaccgcg cagcggggca ggcagggaga
     7741 tgactttacg caaggtaact tcagcgggtt cttgtgaacc aggcagtgcc agatcggcag
     7801 tcgtacaggg gcgcgtgttg cccagtttgc ggcataccgc gtcggtagcc cattcagcca
     7861 tcagccgata ggtcatcagt ttgccaccgg tgatggtgat aaatccgtcc agaccatcgc
     7921 gttcagcatg gtcgagcagc acgatgccac ggctgacgtt acgtccgctc gggtcgtcat
     7981 cgctggcaac cagcgggcgc acgccagaat aggcccgcaa aatgcgcgtt ttcgccatca
     8041 cgggggccag tttttcccct tcacgcagca gaatatcaac ctcttctgcc gtcactcgat
     8101 tatcgtcaat ctcgttgtaa tcaatacgta aagaggtggt accaatcagc gaaatggtat
     8161 cgccaggcac cagaatatcg gcgtcggaag gtttacggca gcggttgatc acatgctggt
     8221 taatgcggtg atccatgatc agcagcgatc ctttcgccgg gaacatgcga atgcgcagat
     8281 cggcatattc ggcaatgtgt tgcccccaga tcccagcggc attaaccacg acaggtgcat
     8341 gaagggcctg agtttcgccg gtgagatggt tacgtacacg aacaccgcac accgtcgcgc
     8401 cttcacgaat cagccccgtg acttcatgag cggtaaggat aacggcaccg tgttctttgg
     8461 catccagcat gtttgctgcg gtcagacgaa atggatcaac ggtgccatcc ggaactttca
     8521 ccgcgccaat cagtgccggg ttaacggcag gttcgataat gcgcgcttgc tgcgggtcta
     8581 tagcttctgc gctgatccct gcttcttcgc aggcgcgaat aaaagtggcc tggaatgaga
     8641 ggtcatcttc cggcagggtg ataaacaggc cgttggttgg ttcaacgcag tgacgtgcaa
     8701 tgcgtttcag gatctggttt tcactaatgc attcgcgggc cgattccgca tcggttactg
     8761 catagcgcgc accgctgtgc agcaggccgt ggttacgccc ggtggcaccg gttgcgatgt
     8821 cgtggcgctc aaccaaaatc acgcgcagcc cgcgcagggc acagtcgcgg gcaatcccgg
     8881 ctcccgttgc gccgccgcca atgataatca cgtcacttga ttgcgagtcg cgagttttca
     8941 ttgttatccc tctgaagttc gttttttacc atttagccat agtaaaaaca tgaattgttt
     9001 gatttcgcgc atattcgctc atgattcgaa agtaaaacgt gatttcatgc gtcattttga
     9061 acattttgta aatcttattt aataatgtgt gcggcaattc acatttaatt tatgaatgtt
     9121 ttcttaacat cgcggcaact caagaaacgg caggttctct cactgaatca ggctgttaat
     9181 cataaataag accacgggcc acggaggcta tcaatgttga gtatttttaa accagcgcca
     9241 cacaaagcgc gcttacctgc cgcggagatc gatccgactt atcgtcgatt gcgctggcaa
     9301 attttcctgg ggatattctt tggctatgcg gcttactatt tggttcgtaa gaactttgcg
     9361 cttgctatgc cttatctggt tgagcaggga ttctcacgcg gtgatttagg ttttgccctt
     9421 tcggggatct cgattgctta tggattttcg aaattcatca tgggttcggt atcggatcgc
     9481 tcgaatccgc gcgttttcct gcccgcaggt ttgattctgg cggcggcagt gatgttgttt
     9541 atgggctttg tgccatgggc gacgtcgagc attgcggtga tgtttgtact gttgttcctc
     9601 tgcggttggt tccaggggat ggggtggccg ccgtgtggtc gtactatggt gcactggtgg
     9661 tcgcagaaag aacgtggcgg cattgtgtca gtgtggaact gtgcgcacaa cgtcggtggt
     9721 ggtattccgc cgctgctgtt cctgctgggg atggcctggt tcaatgactg gcatgcggcg
     9781 ctctatatgc ctgctttctg cgccattctg gtggcattat tcgcctttgc gatgatgcgc
     9841 gataccccgc aatcctgtgg cttgccgccg atcgaagagt acaaaaatga ttatccggac
     9901 gactataacg aaaaagcgga acaggagctg accgcgaagc aaatcttcat gcagtacgtg
     9961 ctgccgaaca aactgctgtg gtacatcgcc atcgccaacg tgttcgttta tctgctgcgt
    10021 tacggcatcc tcgactggtc accgacttat ctgaaagagg ttaagcattt cgcgctggat
    10081 aaatcctcct gggcctactt cttctatgag tatgcgggta ttccgggcac cctgctgtgc
    10141 ggctggatgt cggacaaagt cttccgtggt aaccgtgggg caaccggcgt cttctttatg
    10201 acactggtga ccatcgcgac tatcgtttac tggatgaacc cggcaggtaa cccaaccgtc
    10261 gatatgattt gtatgattgt tatcggcttc ctgatctacg gtcctgtgat gctgatcggt
    10321 ctgcatgcgc tggaactggc accgaaaaaa gcggcaggta cggcagcggg ctttaccggg
    10381 ctgtttggtt acctgggcgg ttcggtggcg gcgagcgcga ttgttggcta caccgtggac
    10441 ttcttcggct gggatggcgg ctttatggta atgattggcg gcagcattct ggcggttatc
    10501 ttgttgattg ttgtgatgat tggcgaaaaa cgtcgccatg aacaattact gcaaaaacgc
    10561 aacggaggct aatggcatga aattgaagct gaaaaacctt agcatggcga tcatgatgag
    10621 cactatagtc atgggaagca gtgcaatggc ggcggacagc aacgaaaaaa tagtcatcgc
    10681 ccatcgcggt gccagtggat atttgccgga gcatacgctg ccagcaaaag cgatggcgta
    10741 tgcgcaggga gcggattatc tggaacagga tttggtgatg accaaagacg accatctggt
    10801 tgttctgcat gaccattatc tcgatcgtgt tactgatgtt gccgatcgtt tcccggatcg
    10861 ggcgcgcaaa gacggtcgtt actacgcgat agatttcacg ctggatgaaa ttaagtcgct
    10921 gaaatttacc gaaggtttcg atattgaaaa cggtaaaaaa gtacagactt atccggggcg
    10981 tttcccaatg ggtaagtccg acttccgggt gcacac
//
LOCUS       DAZSNM010000133        10966 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZSNM010000133 DAZSNM010000000
VERSION     DAZSNM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10966
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.133"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            758..841
                     /gene="dinQ"
                     /locus_tag="ACHW42_002857"
     CDS             758..841
                     /gene="dinQ"
                     /locus_tag="ACHW42_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR8944601.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHW42_002858"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHW42_002858"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR8944602.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHW42_002859"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHW42_002859"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR8944603.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="ACHW42_002860"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="ACHW42_002860"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR8944604.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHW42_002861"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHW42_002861"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR8944605.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="ACHW42_002862"
                     /pseudo
     CDS             complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="ACHW42_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            7850..7903
                     /locus_tag="ACHW42_002863"
     CDS             7850..7903
                     /locus_tag="ACHW42_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944606.1"
                     /translation="MVLNFVLAGDFDCVRRS"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="ACHW42_002864"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="ACHW42_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR8944607.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="ACHW42_002865"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="ACHW42_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR8944608.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="ACHW42_002866"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="ACHW42_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR8944609.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
BASE COUNT         2712 a         2805 c         2919 g         2530 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    10801 aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt
    10861 atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg
    10921 aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaac
//
LOCUS       DAZSNM010000134        10926 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZSNM010000134 DAZSNM010000000
VERSION     DAZSNM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.134"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            157..504
                     /locus_tag="ACHW42_002867"
                     /pseudo
     CDS             157..504
                     /locus_tag="ACHW42_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            557..952
                     /locus_tag="ACHW42_002868"
     CDS             557..952
                     /locus_tag="ACHW42_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR8944610.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="ACHW42_002869"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="ACHW42_002869"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR8944611.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="ACHW42_002870"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="ACHW42_002870"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR8944612.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACHW42_002871"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACHW42_002871"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR8944613.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="ACHW42_002872"
     CDS             complement(5323..6423)
                     /locus_tag="ACHW42_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR8944614.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="ACHW42_002873"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="ACHW42_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR8944615.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="ACHW42_002874"
     CDS             complement(7571..8137)
                     /locus_tag="ACHW42_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR8944616.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="ACHW42_002875"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="ACHW42_002875"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR8944617.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(10128..10172)
                     /locus_tag="ACHW42_002876"
     CDS             complement(10128..10172)
                     /locus_tag="ACHW42_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944618.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            10263..10751
                     /locus_tag="ACHW42_002877"
     CDS             10263..10751
                     /locus_tag="ACHW42_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR8944619.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2669 a         2788 c         2605 g         2864 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgtgtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgc
//
LOCUS       DAZSNM010000135        10896 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZSNM010000135 DAZSNM010000000
VERSION     DAZSNM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10896
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.135"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..492)
                     /locus_tag="ACHW42_002878"
     CDS             complement(<1..492)
                     /locus_tag="ACHW42_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR8944620.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(523..1215)
                     /gene="fimC"
                     /locus_tag="ACHW42_002879"
     CDS             complement(523..1215)
                     /gene="fimC"
                     /locus_tag="ACHW42_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR8944621.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="ACHW42_002880"
     CDS             complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="ACHW42_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR8944622.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2458..3324
                     /gene="folD"
                     /locus_tag="ACHW42_002881"
     CDS             2458..3324
                     /gene="folD"
                     /locus_tag="ACHW42_002881"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR8944623.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3326..3538
                     /gene="ybcJ"
                     /locus_tag="ACHW42_002882"
     CDS             3326..3538
                     /gene="ybcJ"
                     /locus_tag="ACHW42_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR8944624.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3646..4155
                     /locus_tag="ACHW42_002883"
     CDS             3646..4155
                     /locus_tag="ACHW42_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR8944625.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="ACHW42_002884"
     CDS             complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="ACHW42_002884"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR8944626.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5750..6244
                     /gene="ppiB"
                     /locus_tag="ACHW42_002885"
     CDS             5750..6244
                     /gene="ppiB"
                     /locus_tag="ACHW42_002885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR8944627.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6247..6969
                     /gene="lpxH"
                     /locus_tag="ACHW42_002886"
     CDS             6247..6969
                     /gene="lpxH"
                     /locus_tag="ACHW42_002886"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR8944628.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7087..7596
                     /gene="purE"
                     /locus_tag="ACHW42_002887"
     CDS             7087..7596
                     /gene="purE"
                     /locus_tag="ACHW42_002887"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR8944629.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7593..8660
                     /gene="purK"
                     /locus_tag="ACHW42_002888"
     CDS             7593..8660
                     /gene="purK"
                     /locus_tag="ACHW42_002888"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR8944630.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8797..9690)
                     /locus_tag="ACHW42_002889"
     CDS             complement(8797..9690)
                     /locus_tag="ACHW42_002889"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR8944631.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9687..10334)
                     /locus_tag="ACHW42_002890"
                     /pseudo
     CDS             complement(9687..10334)
                     /locus_tag="ACHW42_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10337..>10896
                     /locus_tag="ACHW42_002891"
     CDS             10337..>10896
                     /locus_tag="ACHW42_002891"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HFR8944632.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFV"
BASE COUNT         2617 a         2850 c         2781 g         2648 t
ORIGIN      
        1 agcgctactc aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc
       61 aaagttaatt gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg
      121 ctgggctaac tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt
      181 aaaacagggc attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac
      241 gatatcctgt gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc
      301 aggttgatga ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa
      361 taaaaatgcc gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag
      421 agatgaataa caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt
      481 gggtattttc ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc
      541 tctgaccggg gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac
      601 tcctgagggt aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt
      661 ttggctgccc acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt
      721 gatatagtta ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg
      781 taattgaatt ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt
      841 cctgccgttc aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag
      901 agactcacga tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct
      961 tttgggttca ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct
     1021 gctgttttca atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca
     1081 taccgcagtc tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc
     1141 tccggcagca tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt
     1201 tattttagtc atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat
     1261 aaagaaatga ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg
     1321 catgatacaa agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg
     1381 gtacattact ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc
     1441 gtatctcata ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt
     1501 gcctttataa cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt
     1561 tgagaagcta ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc
     1621 gacacccgtt gctgttgttg tatttgtact ggatacaatg gccaataaat tatcattgat
     1681 tgcatcagcc tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc
     1741 gttcaactga atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc
     1801 ggtacgatat tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc
     1861 agcattcacc agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact
     1921 acccgtagcg gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata
     1981 tattccttca atctcattta tcgactccac atccgtatat aaccgattac tttatttaag
     2041 acactgatag tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca
     2101 ctctattcta aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcac
     2161 cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg
     2221 tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg
     2281 ccatcatgta cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac
     2341 cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct
     2401 tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
     2461 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
     2521 aaagttcagg ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
     2581 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc
     2641 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
     2701 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
     2761 ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac
     2821 ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc
     2881 accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
     2941 gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc
     3001 gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa
     3061 aatgccgatc tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc
     3121 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
     3181 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc
     3241 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
     3301 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
     3361 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
     3421 cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa
     3481 aatcgtcgcc ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc
     3541 cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc
     3601 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
     3661 acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt
     3721 gccggaatca tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt
     3781 gcttacggca atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt
     3841 gtcccgctct tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc
     3901 tggctgtttt taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc
     3961 ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag
     4021 gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg
     4081 attatttccg aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg
     4141 tggcgcagac gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga
     4201 cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca
     4261 cgtgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt
     4321 aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct
     4381 tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt
     4441 gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac
     4501 agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct
     4561 tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag
     4621 aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg
     4681 cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca
     4741 tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc
     4801 attccggagt gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg
     4861 atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg
     4921 ttacccagct gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg
     4981 ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc
     5041 atccggttgc gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga
     5101 tcctgacgcg acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgctg
     5161 ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct
     5221 gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa
     5281 tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca
     5341 ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta
     5401 tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga
     5461 ccgatatgac agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg
     5521 tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa
     5581 gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg
     5641 aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat
     5701 atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt
     5761 ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt
     5821 taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt
     5881 tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac
     5941 caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc
     6001 aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga
     6061 taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc
     6121 tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtaa
     6181 cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga
     6241 gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc
     6301 accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt
     6361 cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag
     6421 atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc
     6481 aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg
     6541 gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg cgacacgctg
     6601 tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa
     6661 atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg
     6721 aacagcaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct
     6781 gtggtcagtg cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc
     6841 ccggcggtgc atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc
     6901 tggcatacgg aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt
     6961 ccgttttaaa aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc
     7021 tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta
     7081 agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc
     7141 gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac
     7201 gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc
     7261 gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc
     7321 atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg
     7381 agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg
     7441 ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg
     7501 actcacgata aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa
     7561 gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg
     7621 ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg
     7681 tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga
     7741 tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga
     7801 accgcgatgt gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc
     7861 tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt
     7921 ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg
     7981 gtcaatggag tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat
     8041 gtattgtcga gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct
     8101 ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc
     8161 gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc
     8221 tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca
     8281 ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga
     8341 cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc
     8401 cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg
     8461 tgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc
     8521 gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg
     8581 cgacgctgga agccttaatc ccgctgctgc cgccagagta tgccagcggc gtgatttggg
     8641 cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct
     8701 tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat
     8761 caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc
     8821 ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg
     8881 gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc
     8941 aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca
     9001 gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc
     9061 ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc
     9121 cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa
     9181 ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa
     9241 atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc
     9301 tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga
     9361 aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct
     9421 ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata
     9481 cggttctacc tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt
     9541 gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact
     9601 ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt
     9661 gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc
     9721 cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc
     9781 gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg
     9841 aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga
     9901 agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac
     9961 caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct
    10021 ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga
    10081 cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa
    10141 agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc
    10201 ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga
    10261 atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg
    10321 aattgcgtgc ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct
    10381 ttcctctttt ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg
    10441 gctggaaacc cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt
    10501 cgatgaagtg gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt
    10561 tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg
    10621 cgcgctggcg gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg
    10681 tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg
    10741 gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc
    10801 caaaggaaat agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc
    10861 actaactccg cgtcaggcta taaaagcctt tgttga
//
LOCUS       DAZSNM010000136        10860 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZSNM010000136 DAZSNM010000000
VERSION     DAZSNM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10860
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.136"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1714
                     /locus_tag="ACHW42_002892"
                     /pseudo
     CDS             1..1714
                     /locus_tag="ACHW42_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(1785..3209)
                     /gene="lpdA"
                     /locus_tag="ACHW42_002893"
     CDS             complement(1785..3209)
                     /gene="lpdA"
                     /locus_tag="ACHW42_002893"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR8944633.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(3534..5414)
                     /gene="aceF"
                     /locus_tag="ACHW42_002894"
     CDS             complement(3534..5414)
                     /gene="aceF"
                     /locus_tag="ACHW42_002894"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR8944634.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(5429..8092)
                     /gene="aceE"
                     /locus_tag="ACHW42_002895"
     CDS             complement(5429..8092)
                     /gene="aceE"
                     /locus_tag="ACHW42_002895"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR8944635.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(8253..9017)
                     /gene="pdhR"
                     /locus_tag="ACHW42_002896"
     CDS             complement(8253..9017)
                     /gene="pdhR"
                     /locus_tag="ACHW42_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR8944636.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            9558..>10860
                     /gene="aroP"
                     /locus_tag="ACHW42_002897"
     CDS             9558..>10860
                     /gene="aroP"
                     /locus_tag="ACHW42_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR8944637.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIP"
BASE COUNT         2479 a         2960 c         2725 g         2696 t
ORIGIN      
        1 gtctgacgct gccgctgtta cgcttcctgc aacggtttcc gcacctcccg taatacccgc
       61 agtcgtcaaa tccgcattca gcactgcaca aatagatcaa tgggtcgcac ccgtcgcgct
      121 gtatcccgac gccctacttt cgcaggtgct gatggcatca acctatccga caaacgttgc
      181 tcaagcagtg caatggtcgc acgataatcc acttaaacaa ggcgatgctg ctattcaggc
      241 ggtatctgac cagccgtggg acgccagcgt taaatcactg gtggcctttc cacaattgat
      301 ggcattgatg ggcgaaaacc cgcaatgggt gcaaaacctg ggcgatgctt ttctggcgca
      361 gccgcaggac gtgatggact cggtacagcg attgcggcaa ctggcgcaac aaaccggctc
      421 gctgaagtca tcaaccgaac agaaagttat taccacaacg aagaaagctg taccggtaaa
      481 acagacagtc acggcacccg tcataccatc caataccgtt ttaactgcca accccgtcat
      541 tacagagccg gcaacaaccg tcatttccat tgagcccgcc aatcctgatg tggtctatat
      601 tcccaactac aacccaaccg tggtttacgg gaactgggcc aatactgcgt atccgccggt
      661 ttatctgcca ccaccagccg gagaaccgtt tgttgacagc tttgtgcgcg gattcggcta
      721 tagcatgggc gttgctacca cgtacgcact attcagcagc atcgactgag atgacgacga
      781 tcatgaccat catcatcatg acgatgatga ttatcatcac cacgatggcg gtcatcgtga
      841 cagtaatggc tggcaacaca acggcgacaa catcaatatc gacgtcaaca atttcaaccg
      901 tatcaccggt gagcatctta ctgataagaa tatggcatgg cggcacaatc caaactaccg
      961 taatggtgtg ccctatcatg atcaggatat ggcaaagcgg tttcatcaaa ccgatgtcaa
     1021 cggcggaatg agtgccacgc agctacctgc tccaacacgc gacagccagc gtcaggcggc
     1081 agcaagtcag tttcagcaac gaacacacgc cgcccccgtc attacacgag atacccaacg
     1141 tcaggcagcg gcacagcggt ttaatgaagc tgaacactat gggagctatg acgacttccg
     1201 cgacttcagc cgtcgccaac cactgaccca gcagcaaaag gacgccgctc gtcagcgtta
     1261 tcagtcggcc tctcctgagc agcgccaggc agtccgcgag aaaatgcaga ctaacccgca
     1321 gatccagcag cgaagggagg cggcgcgcca gcgtattcag tccgcctcgc ctgagcagcg
     1381 ccaggcggtt cgcgagaaaa tgcagactaa cccgcagaac cagcagcgaa gagaggcagc
     1441 gcgtgagcgt attcagtctg caactcccga acagcgccag gtgtttaagg aaaaagtaca
     1501 gcagcgccca ctgaaccaac agcaacgtga taacgcccgc cagcgtatac aatcggcatc
     1561 acctgaacaa cgtcaggttt ttcgggagaa agttcaggag agccgcccac aacgtctaaa
     1621 cgacagtaac catactgtca ggctgaataa cgagcaacgg tcagcagtac gcgaacgtct
     1681 ctctgaacgt ggagcaagaa gactggaaag gtaaattgca gacgtaaaaa aagcggcgtg
     1741 gttagccgct tttttaattg ccggatgttc cggcaaacga aaaattactt cttcttcgct
     1801 ttcgggttcg gcaggtcggt aatgctacct tcgaacactt ctgccgccag gcccacagac
     1861 tcgtgcagag tcgggtgcgc gtggatggtc agtgcgatgt cttcagcatc acaacccatt
     1921 tcgattgcca ggccgatttc acccagcagc tcgccgccgt tggtaccaac aatcgcacca
     1981 ccgatcacac ggtgagattc tttgtcgaaa atcagcttgg tcataccgtc tgcgcagtca
     2041 gaagcgatag cacgaccaga agcagcccac gggaaggtgg cggtttcata gctgatgcct
     2101 ttctctttcg cttctttctc agtcagacct acccatgcaa cttctggttc ggtataggcg
     2161 atggacggga taactttcgg atcgaagtag tgtttcttac cggcgataac ttcagcggca
     2221 acgtgacctt cgtgaacacc tttgtgtgcc agcatcggct gaccgacgat atcgccgata
     2281 gcaaagatgt gcggtacgtt ggtacgcagc tgtttgtcaa cgcggatgaa accacggtcg
     2341 tcaacttcca cgccagcttt gcctgcgtcg aggtttttac cgttcggcac acgaccaatc
     2401 gctaccagca cggcgtcgta acgctgcggt tcagctggtg cttttttgcc ttccatcgtc
     2461 acataaatac cgtcttcttt cgcttcaacg gcggtaactt tggtttccag catcaggttg
     2521 aatttcttgc tgatacgctt ggtgaagact ttaacgatgt ctttgtcagc tgccgggata
     2581 acctggtcga acatttcaac cacgtcaatc tgtgaaccca gcgcgtggta aacggtgccc
     2641 atttccagac cgatgatacc gccacccatt accagcaggc gttctggtac ttctttcagt
     2701 tccagcgcgt cagtggagtc ccagatacgc ggatcttcat gcggaataaa cggcagttgg
     2761 atcgggcgag aacccgctgc aatgatcgca ttgtcgaagt tgatcacggt tttgccgttc
     2821 tcaccttcaa cttccagggt gttagccccg gtgaatttac ccagaccgtt gaccactttg
     2881 actttgcggc ctttcgccat accagccaga ccaccggtca gctgattgat cactttctct
     2941 ttccaggtac gaatcttgtc aatatcggtt ttcggttcgc cgaagacgat accgtgttca
     3001 gccagcgctt tggcttcttc gataactttt gctacgtgca gcagtgcttt agaagggata
     3061 cagccgacgt tcaggcaaac accgccaagg gtgttgtaac gttctacgat tacggtttcc
     3121 agacctaaat cagcgcaacg gaaggcagcg gagtaacctg cggggcctgc cccaagtacc
     3181 acgacctgag ttttgatttc agtactcatc atgacctcta tatatttatc tccggcggtc
     3241 atacccgtcg tctttcaggc cgcagcggcg ttagcttcac gccctcaccc cagtcactta
     3301 cttatgtaag ctcctgggga ttctggcgct tgctaccttg ctgcacccag aaatccatag
     3361 ggtatgtgaa gtaataccct aaccaccacc gggtcgttct atccgtcggt attttacaaa
     3421 attgttaaca atttttaaac aacaaacggc aaccgatttg tctattcgct aataacctca
     3481 atacattcat gagattacca gaaaaaagcc ggccgttggg ccggctcttt tacttacatc
     3541 accagacggc gaatgtcaga cagcgtgttg ttaatgatgg taatgaaacg ggcaccatca
     3601 gcaccgtcga tcacgcggtg gtcgaaggag agagaaatcg gcagcatcag acgcggcacg
     3661 aactctttac cattccacac cggctccatc gcggacttgg aaacgccgag gatagccact
     3721 tccggcgcgt tcacaatcgg cgcgaagtgg gtagtaccca ggccgccgat gctggagatg
     3781 gtgaagcaac cgccctgcat ttcgcccgca gtcagcttac cgtcacgcgc tttcttagaa
     3841 atagtcatca gctcgcgaga cagctcgatg atgcctttct tgttgacgtc tttgaatacc
     3901 ggaacaacca gaccgttcgg ggtatccacc gccacaccga tgttgatgta tttcttcagg
     3961 gtcagacgct gaccgtcttc cgacagcgaa ctattgaagc gaggcatctg ctcaagagct
     4021 gcagcaacgg ctttcatgat gaagacaacc ggggtgatct tcacatccag cttacgtttc
     4081 gccgcttctt cgttctgctg tttacggaac gcttccagct cggtgatatc ggttttgtcg
     4141 aagtgggtaa catgcgggat catcacccag ttacggctca ggttagcacc agaaattttc
     4201 tggatacggc ccagttccac ttcttcgatt tcgccaaact tgctgaagtc caccttcggc
     4261 cacggcagca tgccagggat accaccgcca gtcgccgccg gagctgcttc tgcacgtttg
     4321 atagcttctt tcacgtaagc ctgaacgtct tcgcgcagga tacgaccttt acggccagtg
     4381 cccttcactt tcgccaggtt aacgccaaac tcgcgtgcca gacggcggat cagcggggtc
     4441 gcgtgaacgt aagcgtcgtt ttcagcaaat tcagatttgc cttccgcttt cgcagccgga
     4501 gcttcagctt ttgctgccgg agccggcgct gccgcttcct gtttcgcagg agctgccgca
     4561 ggcgctgcgc cttcaacttc gaagatcata atcagcgaac cagttttcac tttatcgcca
     4621 acgttgactt tcagttcctt cacgacgcct gcaaacggag ccggaacttc catagaagct
     4681 ttatcgcctt ctacggtaat cagtgactgt tcagcggcaa ctttgtcgcc cactttcacc
     4741 atcacttcgg tcacttcaac ttcgtcaccg ccgatatccg gaacgttaac ttctttcacg
     4801 ccagctgctg gtgcaggggt cgctgccgga gtcgcttcct gtttagccgc cggagctgcc
     4861 gcgcctgctt cacccgcgac ttcgaagacc ataatcagcg agccggtaga cactttgtca
     4921 cccacgttca ctttgatctc tttcacggta cctgcaaacg gagccggaac ttccatagaa
     4981 gccttgtcgc cttctacggt gatcagcgac tgttcagctt caactttatc gccaactttc
     5041 accaggattt cggtcacttc aacttcgtcg ctgccgatat ccggaacgtt aacgtctttt
     5101 gccgccgcag ccgcgggtgc tgctgccgga gctgcttctt tcttctcttc tgcctgagca
     5161 ggtgcagcgt ctgctgcacc gtcggcggaa tcgaaaatca taatcagtgc gccggtctgg
     5221 gttttatcgc caacagagac tttgatctct ttaacgatac ccgcctgcgg agatggaact
     5281 tccatagagg ctttgtcgcc ttctacggtg atcagcgact gttcggcttc aactttgtcg
     5341 cccactttga ccaggatctc ggtgatttca acttcatcag ccccgatgtc cggtactttg
     5401 atttcgatag ccattattct tttacctctt acgccagacg cgggttaact ttatctgcat
     5461 cgatgttgaa tttggcgatt gcgtcagcaa ccactttctt atcgatttcg ccacgtttag
     5521 ccagttcgcc cagcgccgca accacgacat aagaagcatc aacttcgaag tggtgacgca
     5581 ggttctcacg gctgtcggaa cgaccgaagc catcagtacc cagtacgcga tagtcgtcag
     5641 ccggtacgta agtacggacc tgctcagcga acagtttcat atagtcggta gatgccactg
     5701 ccggcgcgtc gttcatcacc tgagcgatat acggaacgcg cggagtttcc agcggatgca
     5761 gcatgttcca gcgttcacaa tcctgaccat cacgcgccag ctcggtgaag gaggtcacgc
     5821 tataaacgtc agaacctacg ccgtaatctt tcgccaggat ctctgccgct tcacggacgt
     5881 gacgcaggat ggaaccggag cccagcagct gaactttacc tttgctacct tcaatggttt
     5941 cgagtttgta gataccttta cggatacctt cctcagcacc ttccggcatt gccggcatgt
     6001 ggtagttttc gttcagcgta gtgatgtagt agtaaacgtt ctcttgtttt tcaccgtaca
     6061 tacgctccag accgtcatgc ataatgacag caacttcgta agcgtaagcc gggtcgtaag
     6121 agatacagtt cgggatagtc agcgactgaa tgtggctgtg accatcttcg tgctgcagac
     6181 cttcaccgtt cagggtggta cgaccggaag taccgccgat caggaagcca cgcgcttgct
     6241 ggtctccagc cgcccagcac agatcgccga tacgctggaa gccgaacatc gagtaataga
     6301 tgtagaatgg gatcatcggc agattgttcg tgctgtaaga ggtcgccgct gccagccagg
     6361 aacaccctgc gcccagctcg ttgatccctt cctgcagaat ctgacctttc tcgtcttctt
     6421 tatagtaagc aacctgctcg cggtcctgcg gggtgtactg ctgaccgttc gggctgtaaa
     6481 taccaatctg acggaacaga ccttccatac cgaaagtacg cgcttcgtcg gcgatgatcg
     6541 gtaccagacg atctttgatc gacttgttct tcagcatcac gttcagagca cgaacgaaag
     6601 cgatagtggt agagagctct ttgctctgct cttccagcag cgcgccgaag tcttgcaggc
     6661 tcggcagctc aagcttctcg gtgaagttcg gctgacggct tggcagataa ccgtgcagtt
     6721 tctgacgctg agcgtgcaga taggtatgct cttcagaacc ttccgggaag gtgatgtacg
     6781 gcagtttttc gatatctgca tcagacaccg gcacattgaa acggtcgcgg atgtgacgca
     6841 cgccgtccat gttcattttc ttaacctggt gcgcgatgtt tttaccttca gccgcgtcgc
     6901 ccatgccgta acctttaatg gtatgagcaa ggattactgt cgctttgcct ttggtttcct
     6961 gcgctttctt gaatgcagcg tagattttct tcggatcgtg accaccacgg ttcagtgccc
     7021 agatctgctc gtcagtccag tctgcaacca gtgctgcggt ttcaggatat ttaccgaaga
     7081 agtgttcacg aacgtacgca ccatctttcg atttgaaggt ctggtagtcg ccgtcaacgg
     7141 tttcgttcat cagctggatc agtttaccgc tggtatcttt acgcagcagt tcatcccaac
     7201 ggctacccca catcactttg atcacgttcc agccagcacc ttcgaagatg ccttccagtt
     7261 cgttgatgat cttgccgtta ccggtgaccg ggccgtcaag acgctgcagg ttacagttga
     7321 taacgaaaac caggttatcc agtttttcac gggtagcgat ggtgatcgca cctttggatt
     7381 ccggttcgtc catttcaccg tcgccgagga acgcgtaaac ggtctgttta gaggtatctt
     7441 tcaggccacg gtgttccaga tatttcagga atttagcctg gtaaatagca ccaatcggac
     7501 ccagacccat agatacggtc gggaactgcc agaattccgg catcagtttc gggtgcggat
     7561 aggaagagag gccattgccg tgaacttcct gacggaagtt atccagctgc tcctgagtca
     7621 gacgaccttc caggaaagca cgggcgtaaa cgcccgggga gatgtggccc tggaagtaaa
     7681 ccaggtcgcc gccatcctgc tcgttgcgtg cacggaagaa gtggttaaag cacacatcat
     7741 aaatggttgc ggaagactgg aaggacgcca tgtggccgcc cagttcgagg tcttttttcg
     7801 acgcacgcag caccgtcatg atggcgttcc agcggatagc tgaacgaata cggcgttcca
     7861 gttccagatt acccggatac tccggttgtt cttcaacggg gatggtgttg atgtagttgc
     7921 tgatacctgt gcctgcggct acgtttacac cgcctttgcg ggcttcagca agcagttggt
     7981 cgatcagata ctgagcacgc tcaacacctt cttcacggat gaccgattcg atcgcctgga
     8041 gccagtcgcg agtttcgatc ggatccacgt catttgggaa acgttctgac atgggttatt
     8101 ccttatctat ctaataacgt tgagttttct ggaacctgtc ccattgaact ctcgccagaa
     8161 agctcaataa gacaggttct acgtttagtt accgcgcttt tatatgcgct tgatttacaa
     8221 catcttctgg ataattttta ccagaaaaat cactaattct ttcgttgctc cagacgacgc
     8281 agagaacgct cacggcggct ctcttcacgg cttctgtcga gcaaaatttc ttcgataaag
     8341 gccagatggc gatgcgatgc ttcgcgcgct tcttccggct taccgaccat aatcgcttca
     8401 aatatgcggg tgcggtgact actcaccagc ggcagcatct cgcgacgcga atagagcaat
     8461 tcgaagttct ggcggacatt ctgggccaac atcggctcca tacaccttag cagatgaagc
     8521 agaaccacat tgtgggccgc ttcggtgacg gcaatctgat actggagtac ggcgtttgat
     8581 tccgcgtcca gatcgccaga ctgctgcgcc agctctatgg cgtggtggag ttcacggatg
     8641 cgttccttgt cttcatcggt actacgcagc gcggcgtaat aagcggcgat accttccagg
     8701 gcgtgtcgtg tttcgagcaa gtcatactgt gattcaggat ggtcggagag cagctccacc
     8761 agcggatcgc tgaagctttg ccataggctg ctctggacaa aagtgccgcc accctggcga
     8821 cgaagcaaca agcccttcgc ttcgagacgt tgaatcgcct cacgcaagga gggacgggag
     8881 acgtcgaact gttttgccag ttcgcgttcc ggtgggagtt tttcgcccgg gcggagagtg
     8941 ccttcgagga tcaaaaactc cagttgctgc tcaatcacat cggagagttt tggttggcgg
     9001 attttgctgt aggccatgag ttcctgtctt aagccacttg ccgaagtcaa ttggtcttac
     9061 caatttcatg tctgtgacgc taaagtaaca aagtattcac cttatgtcca tacaggtttt
     9121 gattgaaatc atgaaactgt gcacatttta acaacttgac atatataacg tttcaaagtt
     9181 gtaactatgc acaaatgtag actttacgta ggaaagggat tttttaacga ttattaaact
     9241 ataaaaatcc gaattgaacc gattcactta ccaatttttt gatttttaag tcaataaaaa
     9301 cgaatttaaa ttcactctgc atattgagag gggttgaggc tgagctttac aaacggtttc
     9361 tttttaagca actcatcttc aaccatgcat aaagcgggtg cattcgctgc cgcataccat
     9421 tattcttgat ctgacggaag tctttttgta acaattcaaa cctccttgat gtaaacaaat
     9481 caatacaaca aacggaattg caaacttaca cacgcatcac tgcgtagatc aaaaaaacaa
     9541 tcaccgcacg aggtttcatg atggaaggtc aacagcacgg cgagcagcta aagcgcggcc
     9601 ttaaaaaccg ccatattcag cttatcgcgc tgggtggcgc gatagggaca gggctattcc
     9661 taggtagcgc ctccgtaata cagtccgcag ggccagggat tatcctgggt tacgccattg
     9721 ctggttttat cgcctttctg atcatgcgtc agctgggtga aatggtggtc gaagaacctg
     9781 tcgcaggctc ctttagccac tttgcttata aatactgggg cagttttgcc ggtttcgcct
     9841 ctggctggaa ctactgggta ctgtacgttt tagttgccat ggcagagctg actgccgtgg
     9901 gtaaatacat tcagttctgg tatccggaaa tcccaacctg ggtttctgcc gccgtattct
     9961 ttgtggtgat taacgccatc aacctgacca acgttaaagt gtttggcgag atggagttct
    10021 ggtttgccat tatcaaagtt attgcggtag tagcgatgat catcttcggc ggctggctgc
    10081 tgttcagtgg caacggcggc ccgcaggcga gcgttagcaa cctgtgggat caaggtggtt
    10141 tcctgccgca cggcttcacc ggactggtga tgatgatggc gattatcatg ttctcgttcg
    10201 gtggtctgga actggtgggg atcaccgcag cagaagctga taacccggag caaagtatcc
    10261 cgaaagcaac taaccaggtt atctaccgca tcctgatttt ctatattggt tcgttagccg
    10321 ttctgctctc actgatgccg tggacccgcg ttaccgccga taccagtccg tttgtgctga
    10381 tcttccacga gttaggcgat acctttgtgg cgaatgcgct gaacatcgtg gtactgactg
    10441 cggcgctctc cgtgtacaac agctgcgtat attgcaacag ccgtatgctg tttggtctgg
    10501 cacaacaggg gaacgcgcca aaagcgctgg cgtctgtcga taaacgcggc gtaccggtta
    10561 acaccattct ggtgtctgcg ctggttacag cattgtgcgt attgattaac tatcttgctc
    10621 cggaatccgc atttggcctg ttaatggcac tggtggtatc cgcactggtg atcaactggg
    10681 cgatgatcag tctggcgcat atgaagttcc gtcgcgccaa gcaggaacaa ggcgtggtaa
    10741 ctcgcttccc tgctctgctt tatccgctgg gtaactggat ctgcctgctg tttatggcgg
    10801 cggtactggt gattatgctg atgaccccag gaatggcgat ttcggtatac ctgatcccgg
//
LOCUS       DAZSNM010000137        10842 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZSNM010000137 DAZSNM010000000
VERSION     DAZSNM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10842
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.137"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..191
                     /locus_tag="ACHW42_002898"
     CDS             <1..191
                     /locus_tag="ACHW42_002898"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944638.1"
                     /translation="PPHVKALPDTITQTSIHRTLQFIEFARSCRPDKAFTPHPAINRR
                     RLPNGRFFTYSPPSNVPA"
     gene            complement(157..1392)
                     /locus_tag="ACHW42_002899"
     CDS             complement(157..1392)
                     /locus_tag="ACHW42_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR8944639.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1539..1615)
                     /locus_tag="ACHW42_002900"
     tRNA            complement(1539..1615)
                     /locus_tag="ACHW42_002900"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Pro,seq:tgg)
     gene            complement(1658..1744)
                     /locus_tag="ACHW42_002901"
     tRNA            complement(1658..1744)
                     /locus_tag="ACHW42_002901"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1708..1710),aa:Leu,seq:cag)
     gene            complement(1765..1840)
                     /locus_tag="ACHW42_002902"
     tRNA            complement(1765..1840)
                     /locus_tag="ACHW42_002902"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1805..1807),aa:His,seq:gtg)
     gene            complement(1899..1975)
                     /locus_tag="ACHW42_002903"
     tRNA            complement(1899..1975)
                     /locus_tag="ACHW42_002903"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1939..1941),aa:Arg,seq:ccg)
     gene            complement(2078..3463)
                     /locus_tag="ACHW42_002904"
     CDS             complement(2078..3463)
                     /locus_tag="ACHW42_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8944640.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3654..4394)
                     /gene="wecG"
                     /locus_tag="ACHW42_002905"
     CDS             complement(3654..4394)
                     /gene="wecG"
                     /locus_tag="ACHW42_002905"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR8944641.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4397..5749)
                     /gene="wzyE"
                     /locus_tag="ACHW42_002906"
     CDS             complement(4397..5749)
                     /gene="wzyE"
                     /locus_tag="ACHW42_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR8944642.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5746..6825)
                     /gene="rffT"
                     /locus_tag="ACHW42_002907"
                     /gene_synonym="wecF"
     CDS             complement(5746..6825)
                     /gene="rffT"
                     /locus_tag="ACHW42_002907"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR8944643.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6822..8072)
                     /gene="wzxE"
                     /locus_tag="ACHW42_002908"
     CDS             complement(6822..8072)
                     /gene="wzxE"
                     /locus_tag="ACHW42_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR8944644.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8074..9204)
                     /gene="rffA"
                     /locus_tag="ACHW42_002909"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8074..9204)
                     /gene="rffA"
                     /locus_tag="ACHW42_002909"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR8944645.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9209..9883)
                     /gene="rffC"
                     /locus_tag="ACHW42_002910"
                     /gene_synonym="wecD"
     CDS             complement(9209..9883)
                     /gene="rffC"
                     /locus_tag="ACHW42_002910"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR8944646.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9861..10742)
                     /gene="rfbA"
                     /locus_tag="ACHW42_002911"
     CDS             complement(9861..10742)
                     /gene="rfbA"
                     /locus_tag="ACHW42_002911"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR8944647.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10761..>10842)
                     /locus_tag="ACHW42_002912"
     CDS             complement(10761..>10842)
                     /locus_tag="ACHW42_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944648.1"
                     /translation="WYLANESWWKQVQDGSYQGERLGLKG"
BASE COUNT         2816 a         3090 c         2640 g         2296 t
ORIGIN      
        1 tgccaccgca cgtaaaggct ttgccggata cgatcaccca aaccagtatc cacagaacgc
       61 tgcaatttat tgaatttgca cgatcatgta ggccggataa ggcgtttacg ccgcatccgg
      121 caatcaaccg caggcggctt ccaaatggcc gcttttttac ttattcacca ccatcaaatg
      181 tgcctgcata atgtcactgg ccgggcgacc gtgcgccagc aaatcagcca ttgctttaag
      241 atatggcggt agatggcgga aataacgctg atacccggca cacaaataat tcagccccgg
      301 tttgccgctg gcatcgagca tgaagcggtg tttcgggcag cctccccaac atgcttttaa
      361 cacgttacaa ctgcgacact gcgccggtaa ttgcttaaat ttatcttcac caaacacctg
      421 ctgttgcggg gaatcgatca tttctgcaat tgtttgctga tgcatattcc ccagccgata
      481 ttgcggataa acatagtgat cgcaggcgta aacgtcgccg ttgtgctcaa caatcaccga
      541 gcgcccacag gttggctgat gatggcaaac cgcacccggc gcaccgacaa aattggcaaa
      601 cgcccattcg atattcatca cgaaaatctt gccgacgtcg cgtttgatcc agtggtcaaa
      661 aatcgccacc agaaattcac caaattcatc ggggcgcaca gaccattccg tcagttcccc
      721 ctgaatatca ccaggcgcat gtagtttcag tccatcgcgg acagctgttt catcagccag
      781 gcgctcgacc accggaataa actggatgaa ttcgactccc gcatcgcaca aaaaatcata
      841 aacctgtaac ggttgctgcg cgctggtgcg gttgacgcag accagcacgt tatagtcgac
      901 atgatgtttt tgcaggagcg tcagggcacg catcaccagt ttatgcgtgg gtctgccgcc
      961 tttggtcacg cgatattgat tgtggatctc agccggacca tccagcgata acccaacaag
     1021 aaaattattt tctgccagaa atgcacacca ttcgtcatcg agcagcacgc cgttagtctg
     1081 gaaactgtta cttatcttcc tgccagcacc atatttcgcc tgtagcgcca cggcacggcg
     1141 gtaaaactcc agcccgagta gcgttggttc gccgccctgc caggtaaaag ccacttcgtt
     1201 ttgcagttcg ctggcggcga tatagtgacg gatatacgct tccagcgtgt catcgtccat
     1261 atgcgtgact ggcttttcgc ggtaaaggga ttgtttttcg agataaaaac agtagtcaca
     1321 gttcagatta caatcggaac cgctcggttt cgctatcaca tgaaaagcac gcgttggaac
     1381 ctgttgcagc atggtcgctc cttgcctgaa gtcatccttc aatacgttaa gggcgaaatc
     1441 ataccgtggg gcaatgagtt attacgggcg tttattgcga agggagggtg acgaaatgca
     1501 cagaaaacaa aaaaccccgc cgaagcgggg ttcaaaattg gtcggcgaga gaggattcga
     1561 acctccgacc cactggtccc aaaccagttg cgctaccaag ctgcgctact cgccgaaata
     1621 ctgctttttg aatttttagt tcaattcttt aaagtcgtgg tgcgaggggg gggacttgaa
     1681 cccccacgtc cgtaaggaca ctaacacctg aagctagcgc gtctaccaat tccgccacct
     1741 tcgcattgtc acaacttcta ataatggggt ggctaatggg attcgaaccc acgacaactg
     1801 gaatcacaat ccagggctct accaactgag ctatagccac cactacaaat cttgttacgc
     1861 ggtattacta ccaccgcagc tcaagcgctg ggactaaatg gcgcgcccga caggattcga
     1921 acctgagacc tctgcctccg gagggcagcg ctctatccag ctgagctacg ggcgcttagc
     1981 gccgttgcgg gggtggataa tacggacttc ccacccctct gtctagtccc tttttaaata
     2041 aattgctcgt ttggttacgg tttgtgcgtt ttgctgctta ttcctccagt ttatgcgctt
     2101 taccgtggcg attaagccca aaaactttat aaatcgccgt caccgccagc ataaagatga
     2161 tgccgacaaa cagcgacata cgcgtatctt cattaaagta catgccgatc aaaacgcaaa
     2221 tcaggaatgc cattgttacg tagttggccc acgggaacag gattgagcgg aacggatggc
     2281 tggcaatcgc cgctttatgt gcacgccgaa aacgcagctg gcttatcaat atcacaaacc
     2341 atggcaccat ccccggaagc acgctggcac tgtagacgta gacaaacaca cgctgcggat
     2401 tgggaatgat gtagttcagg catgagccaa ttagcagaat agcaatagat actgccacac
     2461 ccgcaaccgg tacgccgtga cgggaaactt ttgccattgc cgccggtaac tgacggtttt
     2521 tcgccagtgc gtagagcata cgtccgcaac tgtacatgcc gctgttacag ccagagagcg
     2581 cagccgtcag caccacaaag ttgataatgc ccgccgctgc ggtaataccg attttggcaa
     2641 aagtcagtac gaacgggctg ccgttgctgc caatttcatt ccacgggaag atggtgacga
     2701 taacgaaaat cgcgcctacg tagaaaatca ggatccgcca cagcactttg cctacggcac
     2761 tgcgcagcgt cacctgcgga ttcttcgctt caccggcagt aatgccaatc agctccacgc
     2821 cctggtagga cgccaccaca atacacagag cggtcaggaa ccctttccag ccacccgcaa
     2881 agaaaccgcc atgctctgtg agattgctaa aaccaatcga ctgcccgcca ttgccaaagc
     2941 caaagaaaat cacgcccagg ccaatgacaa tcatcacgat aatcgtggtg actttgatca
     3001 tcgcgaacca gaactcgatt tcgccgtaca accgtaccgc cgccagattc gccaacgcca
     3061 ccagcgccac cgcgatcaat gcgggtatcc actgcgccat ctccggggac caaaactgga
     3121 cataaacgcc aatggcggtg atttctgaga tccccaccgc catccacata aaccagtaag
     3181 accaggcggt gagatagcca aaaaacgggc tcatataacg atgcgcataa acggcgaacg
     3241 aaccggtaac cggttcgagg aacaacattt cgcccattga acgcatgatg aaaaaaacga
     3301 acagcccggc gatgatatag gccaacaata cggatggccc ggcccatttc agggtactgg
     3361 cggcccccat aaacaggccg acgccaatgg tgccccccag ggcgatgagt tcgatatgtc
     3421 gagcttccag cccacgctgt agctctggtt tgttatctgc cataaatcct cgtgttgtgt
     3481 ttgcatgctt tccggtgtta ccggttatcg ttatgggtac atcgagtgtt gcgaatgttt
     3541 tcgtaattca ggagaaatgg caaataaagc attaaaaatt tgaatgcttt gtgtaataaa
     3601 aaagcagaca ggcgacgaag tgaccactcc gtcgctttac aaagagagga aaatcagagg
     3661 ttgccggtgt agtgccagcg taaataacgc agcaaacgaa gctgacgctt aatgcggctc
     3721 ggctgcgaaa gcaggcggta gagccactcc agtcccagcg tttgccagat tttcggtgcg
     3781 cgttttacgt gaccggtgaa aacatcgtaa gtcccgccaa cgcccatata cagcgcatct
     3841 ggatgcacca gacggcagtc gcgcatgaag atctcctgct ttggcgatcc catcgcaacg
     3901 gtgacgattt gcgctccgct ggcatgaatg cgttcaaaca gcgcctgacg ctgctcgggt
     3961 ttaaaataac catcctgact gccaacgata ttcacattcc attggttgcg cagtttagct
     4021 tcagtttgcg ccagcacttc aggtttaccg cccacaagaa ataccggcgt cccttctttg
     4081 cctgcgcgcg ccatcagctc ttcccagaga tcggcaccgg caacgcggga aacctgcgcc
     4141 tgcgggtact ttttacgtac tgaacgtaca atgctgatgc catccgcata tttaaattcg
     4201 gcagcgttaa ttaactccct gacctcggcg ttatcttcaa tggtcagcat tttttcggca
     4261 ttaatggcaa ccagcgttcc ctgcttaagc tgcccgtcag caaacagata atcgagggca
     4321 tgctgcatat cacgccaacc aatcaactgt aagccacgca gcgtataggt cggtgccgtg
     4381 gtgttgttat tcattgttat ccttcaacct gcgtccggag cgatgatttt gtacgtttat
     4441 gaatgagtcc ggcgctttca aaaagccagt acaacagttt tgcgatcatc agacatgcgc
     4501 cgaagaccac gataaaaaag actacgcgtg agacaaacga atccagccct tcacgtgcca
     4561 gcacgatcat attgaagatc gccccaaagc agaaactgtg caatatcgca gccttatagc
     4621 gattagtctc gcggttgccc agctcataca gccagtcgaa ccatttgatg atcagtccaa
     4681 ccacaatcgc cccgagtggg atgaacaacg cgccgcccat caccaccagt gagcctataa
     4741 gcgtaggcga gatcgccagt ccggagtggt tattcagtac ttcccaggta aagtaattgg
     4801 ctgagttcag caccatactc gggcgccccg gccacagcca ggaagggata aagacataga
     4861 aatcgcggac aattggagcc aggccctgga agtcgatgtt gtcgtagttc tgcaacagca
     4921 acgccagatt ctcccacggt gaaaaggtgt cgcgagtgag atagagaaac gtatagaacg
     4981 cttcatcgcc gctcacattc attccatagc gttttaacgc cagccagaac atgccaacaa
     5041 tccccagcac gcccgccgcc gccagcatcc acaacgaaat ccagccgcga ataatgccaa
     5101 taaacaggaa gatagcgaat gcgatgatga tattggcgcg agtgccgccg acaatcatat
     5161 aagtcagcaa gccaaaggcg accgtgctga cgaggaaaaa cagccacgct ttgctgtcct
     5221 ggcgcagaaa gtagaccacc agcatcgccg ggatgaaaaa gtaaaagaag cgttttaacg
     5281 ccacgccgga gacttcactg gaaaagatct gactgtagga gttaagccgg aacagcaaaa
     5341 agccgttgtg catgaagaag atgccgacgc ttaccagcgc gatacccatc aggatcaccc
     5401 acgtaagatt ggtctccacg cggttcatgg taaacagcgg acggcgcggt acatcagcaa
     5461 cgcgtttgcg taggcgggtt ttgtaggtga catagtaaac cgcgtagaag cagcccgcag
     5521 aaagcaacac ctgcaacaag atttctggcg gcgcgacacc aacatcaaag cgaaatacca
     5581 gcacgctggt cagcgggaag ccgaagaaaa aggtgagcaa aaacagcaat gaaaagaaga
     5641 cattgaagtt aaagcgcaca cggcgaaact caaaccaggt cagcgtggca ataaacagcg
     5701 tgcagagcag ccagaccaca aacaggccac tgaattgcag cagactcatg cgacctcccc
     5761 ggcggcaatc gccaacgccc gctgccagcc ttgtagatag ttagggctaa agaaggcaat
     5821 ggtgttttta tccgccgacg ccaactggcg ctgcgcttca cgcacaatat cctcgttgag
     5881 atcgtcggta gtaaacagca ctggcaaatg ctgttccgtc atatcctgcc agaacggatt
     5941 ttccctgtta agcacacaag gaatgcccgc ctgaatcagt aagcaaagcg taccaatgcc
     6001 ctgctggcgg gcaaaaataa agtaaccaag atcgcactga cgaagtagcg ccagataggc
     6061 gtcaaattcc agtttttcgc tcaaaatttg cagattttct tcgctgaata actccagccc
     6121 cgcctgacga acttcctcaa tgtacgcttc gttattaggc ggatatccca tcggcaccac
     6181 cacttttacc gtatcgccaa attgctgatg aacggcacgt aaagcggcga catgctcatt
     6241 gctgcggtcg ccggagttcc ccaccagaat ggtcattttt ccttcacgtt gccgatcgtt
     6301 cgccatcgta ttgagagaag ggtccatccg cgtcgggaag tacagcagtt ctccccgcac
     6361 ctttgggtgt gttttggcaa aaaagctcaa atcgccgcgg gtggcaaata cacagccgac
     6421 tcgcttttgc gccaggcgac gtagtgggta aaaaagctta tatctcaagc cactggaaag
     6481 ctcatacagg tctgcccccc agatatgcca gaaaaactgg ctgggcttaa tgccaccact
     6541 cagcagagcc agccacagtg tgggattgaa ctgaccgtgg aaaaaaaatc gctgctgacg
     6601 gttagctttt gctttcgcga tgaccgcttc cgccagcgat tttttcccag ggaaaaattg
     6661 cacagaaagc gccggacagc tatcacttaa gccgtcgtcc ttgccaacaa ccataaactc
     6721 gcgcgcgtgc tcgctcgtcg cggccagcgc gtcattgaaa aaccgcaaaa cggttcggtt
     6781 atggtgaggg atatccgatc ccagtacgtg aatcagtaca gtcatgcccg cctacgccag
     6841 agtaaaaaca cgccacaaca aagagaaaaa tagacgatat aagttgccat atatgcctgc
     6901 gccgcgccca gtgcaccatg cgcagggatt agccagtggg caaataccat caataaagtg
     6961 aactggctga cttccgccag aatataaaac cgcagtgacg ctttggcgat caccagataa
     7021 ccaaagacat aagcgcccac ttttaacaca tcacccacta actgccaggc aaagagatcg
     7081 cgcatagcgg taaatttatt cgacaacagc aaccagatag caaaatcacg cagcaaccag
     7141 acggtaaaac tcgctgccgc cactgccggt aagacgaatt tcagcgattt aaccacttcc
     7201 cgggtgatat cgcgcttttc cgttagccgc gacaacgtgg gcagcaaata tacgctgaac
     7261 gatgccgtaa taaattgcag gtaggcatcg gaaatactgc tcaccccttg ccagatcccc
     7321 acttcatccc agctatactg cgccgccagc agtttacgca tcatgatgta agcaacaggc
     7381 aaggtcaccg acgtaatcaa cgccatgagc gtaaatttgc tcaactgccc tgccagaccg
     7441 ttatcccagc tgggtttcag atagcttaac gggatgacac cacgtttaat caacatgatg
     7501 gcggcaggaa ttaccaccag cgcgggaatc agcgccagac ccagcaacgc cccttcataa
     7561 ccgcccaaac ggtaactgac gtagtacgcg agaacgccaa tcaagctgcc gacaatcagc
     7621 gataacgcat tacctgcggc atcgcgaaag cctttcatca gcgccagtaa caggttgccc
     7681 caggcgatcc ccatttgcac cagcgccact aaacgcacca gcccctgata gtcggtatta
     7741 ccaaacaatc cctggctgat tggcgcagct gccagcacaa aaaccagcgc catcagcgta
     7801 gagaaaccaa gtaccatcgc tgatgaagtg ccgaccacgc ggcgcagctg ttgcggatta
     7861 tcatggtact gggcaacgta tttggttaca ccgttaaaga tgccagcccc ggcaagcacg
     7921 ccgagcacgg taatcaactg gcggaaattt gccgccagcc caagccccgc cggaccaaat
     7981 gacaccgcca gcaacttacc gaccagtaac ccggcaccaa tcttgaccag tgtactggcc
     8041 gccgtccaca aggacgcttt tgccaacgac atatcaggag aagtagttca acaaagtcgc
     8101 aattaccgta cgctgattga cgggcgacag gttgtagaac agcggcaggc gcagcaggcg
     8161 ctcgctctct ttggtggtgt agcgatcttc accgtggaac tcaccaaagt gttcccccgc
     8221 agggcaaccg tgcagcggaa tgtaatgaaa caccgccatg atttccgctt ctttcagaaa
     8281 gttaatcaac gcgctccggt catcaatatc ccgcagttta atgtagaaca tatgcgcgtt
     8341 ctgcacgcag ccatcgggaa tcgacggcag ctcgatacgc ccggctttcg ccagaggcgc
     8401 taacgtatcg tagtagtttt gccacagcgc cagacgttgc tggttgatac gatccgctgc
     8461 ttccagttgc gcccacaggt acgcagcttg cagatcggac atcaaatagc tggagccaat
     8521 atcgcgccag gtatatttat cgacctgacc acggaagaac tggctgcggt tagtgccctt
     8581 ttcacggatg atctcggctc gttcgattaa cgctttatcg ttaatcagcg tcgcgccgcc
     8641 ttcaccaccc gccgtgtagt ttttggtttc atggaagcta aagcagccaa tatgaccaat
     8701 ggttcccagt gcacgccctt tgtaagtgga catcacgccc tgagcggcat cttccaccac
     8761 aaacaaatta tgctttttcg ccaacgccat aatggtgtcc atttcgcagg ccacacccgc
     8821 gtaatggacc ggcacgataa cgcgcgtttt gtcggtgatc gctgcttcaa tcaacgtttc
     8881 gtcgatgttc atggtgtccg ggcgaacatc cacaaagacg attttcgcgc cacgcagcac
     8941 aaaggcattg gcggtggaga caaaggtgta gctcggcatg atcacttcat cgccaggctg
     9001 gatatcgagc agcagcgccg ccatctccag cgaagcggtg caggacggcg tcagtaacac
     9061 tttggcgctg ccaaaacgtt gctccagcca ctgctggcag cgacgggtaa aaccgccatc
     9121 gccacacagt ttgccgctac ccattgccga ctgcatatag tcgagttcgg ttcccaccac
     9181 cggcggtgcg ttaaatggaa tcatgtgatc acctgtataa ccagtacgcg gtgctttcta
     9241 cattcgcacc actttgtatg tatcgtttaa gcgcggcggt gttgcccatt tgggtcgcca
     9301 cccgcaaagt tgttttaccg cgagcatacg cccagtttag cgccgtttgc atcagctcag
     9361 cacctgcacc gcgtccagcc agcaggccaa ttcgcgcatc tgtcgcattg agttcccgta
     9421 aagagacata gccgcgaata tcgccggacg ccgcacgtag aatcagacat tgatgatcaa
     9481 aggtgccgcg cacggcattt tcaatccact gtgcataaaa gcgaccgctg gcgtcaggcg
     9541 cataccacgg cgcacgaaaa cggctttgcg caaatgcggc gctagctaac tgacgtaatg
     9601 cgggaatatc ggtctcttgt gccacgacag caccgctatc actgacattg ttcacgggta
     9661 gcgccaaatc aacttcacct tctaccaggg agaatcccag ctgttgcagg gcatccagtt
     9721 cccccgtatt tgatgccgca attttggcct gcacccgtga ccatggcgct aaggcgtctg
     9781 acgtcaggag cggtgcttca gacgtaatgc gcacgatggc gctgttaaca ccaaagaagg
     9841 cgttttccca ggttagtggc tcaatactgg cgcggacggg cacgaagtaa ctccagcaga
     9901 tattggccgt agccagtttt cgctaatgaa ctggcagcac gcttcacacc ctcgtcatcg
     9961 agccagccgt tacgccaggc gatctcttcc aggcaggcaa tcttaaagcc ctggcgtttt
    10021 tctactgtct gcacaaaagt gctggcctca atcaggctgt cgtgagtgcc ggtatccagc
    10081 caggcaaatc cgcgcccgag cagttcaacg gtcaggttgc ccgcctcgag gtacatctgg
    10141 ttgatggagg taatctccag ttcaccgcgc tccgacggct tcacctgctt tgcgtactcc
    10201 acgactttac tgtcgtagaa ataaagcccg gtcaccgccc agtttgactt cggctgtttt
    10261 ggcttttctt caagcgagat agcgcggaaa ttgtcgtcaa attccaccac gccaaagcgt
    10321 tccgggtcca tcacctgata gccaaaaacc gtcgcccctt ccgtgcgcgc cgcaacatga
    10381 cgcagcttcg gactgaagcc ctgaccgaag aagatgttat cgcccagcac cagacaagaa
    10441 ggttcaccat taaggaaggt ttcaccgatg ataaaggcct gcgccagacc gtccgggctg
    10501 ggctgttcgg catattccag ctggataccg aactcactac catcgcccag caggcgctgg
    10561 aaataacctt tatcttccgg cgtagtgatg atgagaattt cgcggatacc ggccagcatc
    10621 agcaccgaca gcggatagta aatcattggc ttatcgtaaa tcggcaacag ttgcttcgat
    10681 acgccgcgcg taatcggatg caatcgggta ccggaaccgc ccgccaggat aatacccttc
    10741 atgtttgcct ccgctgaaaa ttagcctttc agacctaaac gctcaccctg atagctgccg
    10801 tcctgcacct gcttccacca gctttcatta gccagatacc ac
//
LOCUS       DAZSNM010000138        10829 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZSNM010000138 DAZSNM010000000
VERSION     DAZSNM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10829
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.138"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            61..822
                     /gene="adiY"
                     /locus_tag="ACHW42_002913"
     CDS             61..822
                     /gene="adiY"
                     /locus_tag="ACHW42_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR8944649.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            959..2296
                     /gene="adiC"
                     /locus_tag="ACHW42_002914"
     CDS             959..2296
                     /gene="adiC"
                     /locus_tag="ACHW42_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR8944650.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2400..4043
                     /gene="eptA"
                     /locus_tag="ACHW42_002915"
     CDS             2400..4043
                     /gene="eptA"
                     /locus_tag="ACHW42_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR8944651.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            4040..4708
                     /gene="basR"
                     /locus_tag="ACHW42_002916"
     CDS             4040..4708
                     /gene="basR"
                     /locus_tag="ACHW42_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR8944652.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4709..5809
                     /gene="pmrB"
                     /locus_tag="ACHW42_002917"
     CDS             4709..5809
                     /gene="pmrB"
                     /locus_tag="ACHW42_002917"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR8944653.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5785..5874)
                     /gene="pmrR"
                     /locus_tag="ACHW42_002918"
     CDS             complement(5785..5874)
                     /gene="pmrR"
                     /locus_tag="ACHW42_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR8944654.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5986..7488)
                     /gene="proP"
                     /locus_tag="ACHW42_002919"
     CDS             complement(5986..7488)
                     /gene="proP"
                     /locus_tag="ACHW42_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR8944655.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7752..8630)
                     /locus_tag="ACHW42_002920"
     CDS             complement(7752..8630)
                     /locus_tag="ACHW42_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR8944656.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFDDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(8627..>10829)
                     /gene="crfC"
                     /locus_tag="ACHW42_002921"
     CDS             complement(8627..>10829)
                     /gene="crfC"
                     /locus_tag="ACHW42_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR8944657.1"
                     /translation="SQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLALPWSQPNIAE
                     RHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPMTALPTLIRH
                     TPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRALMQRIENGVA
                     FEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVEFVHLAGLES
                     YPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISDEEVREAILA
                     VGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVSSMWGYLANR
                     ARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQLWEDSLFAQ
                     PIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQLRQNIHQVE
                     ESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQDDSEPLSEIR
                     TRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELSESGFRPGFH
                     FPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPDWGRGNEKSP
                     TETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFASCLTELQTRL
                     RESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAERY"
BASE COUNT         2702 a         2845 c         2671 g         2611 t
ORIGIN      
        1 tcccggtatc gctttgcttt agcgagaact ggtcttttat tcgcactcag gagtacatgt
       61 atgaggattt gcagcgacca accttgtatt gttttattga ctgaaaaaga tgtctggata
      121 agggtgaatg ggaaagaacc tattagcctt aaagctaacc atatggcgtt attaaattgt
      181 gaaaataata ttatcgacgt ctcctctctt aacaacactt tggttgctca tattagtcac
      241 gacatcatca aagattacct ccggtttctg aataaagatc tctcgcaaat accagtatgg
      301 caacgtagcg ctacgcccat actcaccctg ccatgcctga cgccagacgt ctttcgcgtt
      361 gccgcgcaac acagcatgat gcccgcagaa actgagtcag aaaaggaacg aacacgcgca
      421 ttattattca cggtgctatc ccgttttctc gacagtaaaa aattcctttc attaatgatg
      481 tatatgttac gtaattgtgt aagtgacagc gtttatcaaa ttattgaaag cgatattcac
      541 aaagactgga atcttagtat ggtagccagt tgtttatgtc ttagcccaag tctgttaaag
      601 aaaaagttga aaagcgaaaa caccagttat agccaaataa tcaccacctg ccgcatgcgt
      661 tatgccgtaa atgaattaat gatggacggt aaaaatatct ctcaggtatc acagtcctgc
      721 ggctacaaca gtacgtcgta ctttatttct gtctttaaag acttctacgg tatgacgccg
      781 ctgcattatg ttagtcagca cagagaacgc actgtcgcct gatttttaac cttaacgaag
      841 agctatatta ataacggcat cagcgataac ccggtcgata ataattcaac tatcgaatgc
      901 aggcgtatga tatgacgtaa tttattgtca cgaagctcgc cttcgcagga gtttaattat
      961 gtcttcggat gctgatgctc acaaagtggg cttaatcccc gtcaccctga tggtgtcggg
     1021 gaatattatg gggtcaggtg tttttctgtt acctgcaaac ctggcctcca ctggcgggat
     1081 tgccatttat ggatggttgg tgacgattat cggtgcgctg gggctctcga tggtatacgc
     1141 caaaatgtcg ttcctcgacc caagtcctgg tggttcttac gcttacgccc gccgctgctt
     1201 tggcccgttt ctcggttatc aaaccaacgt cctctactgg ctggcctgct ggatcggcaa
     1261 tatcgccatg gtggtcattg gcgtaggata tttaagttac ttcttcccga ttctgaaaga
     1321 tccattggta ttaaccatca cctgcgtcgt ggtgctgtgg atcttcgtcc tgctgaacat
     1381 tgtcggtccg aaaatgatca cccgcgtgca ggccgttgcc actgtgctgg cgctgatccc
     1441 catcgtcggg attgccgtat ttggctggtt ctggttccgt ggtgaaacct atatggcggc
     1501 atggaacgtc agcggcctgg gcaccttcgg tgcgattcaa agtaccctta acgttacgct
     1561 gtggtcgttc atcggtgtgg aaagtgcctc cgttgccgca ggtgtggtga aaaacccgaa
     1621 acgcaatgtc cctatcgcca ccattggtgg ggtattgatt gccgccgttt gctatgtgct
     1681 ctccaccacc gcgattatgg ggatgatccc taatgccgca ctgcgcgttt ctgcctcgcc
     1741 attcggtgat gccgcacgga tggcgttggg tgacaccgct ggggccattg tttccttctg
     1801 cgcagctgcg ggttgcttag gttcactggg cggctggacg ttgctggcgg gtcaaacggc
     1861 gaaagccgct gccgatgacg gactgttccc accgattttt gcccgtgtaa ataaagcggg
     1921 tacgccagtg gcggggctga ttatcgtcgg tattttgatg accatcttcc agctcagcag
     1981 catttcacca aacgcgacca aagagttcgg tctggtttct tccgtctcgg tcatctttac
     2041 actggtgcca tatctttaca cctgtgcggc gttactgctg ctcggccacg gtcactttgg
     2101 taaagcacgc ccggcatatc tggctgttac taccattgcc ttcctctact gcatttgggc
     2161 cgtggtgggg tccggggcga aagaggttat gtggtcattt gtcaccctga tggtcatcac
     2221 cgccatgtat gcgctgaatt acaaccggct acataaaaac ccgtatccct tagatgcacc
     2281 aataagcaaa gattaattcc ccttaatcca gcaaacataa aagccaacct taagaactta
     2341 aggttggctt aattttgctt tgcgagcata tgcgcacttt gttcgatgga aacaccgtga
     2401 tgttgaagcg cctactaaaa agaccctctt tgaatttact cgcctggcta ttgttggccg
     2461 ctttttatat ctctatctgc ctgaatattg ccttttttaa acaggtgttg caggcgctgc
     2521 cgctggattc gctgcataac gtactggttt tcttgtcgat gccggttgtc gctttcagcg
     2581 tgattaatat tgtcctgaca ctaagctctt tcttatggct taatcgacca ctggcctgcc
     2641 tgtttattct gattggcgcg gctgcacaat atttcataat gacttacggc atcgtcatcg
     2701 accgctcgat gattgccaat attattgata ccactccagc agaaagttat gcgctgatga
     2761 caccgcaaat gttattaacg ctgggattca gcggcgtgct tgctgagctg attgcctgct
     2821 ggctaaaaat caaacctgcc acctcgcgtc tgcgcagtgt tcttttccgt ggagccaata
     2881 ttctggtttc tgtactgctg attttgctgg tcgccgcact gttttataaa gactacgcct
     2941 cgttgttccg caataacaaa gagctggtga aatccttaag cccctctaac agcattgttg
     3001 ccagctggtc atggtactcc catcagcgac tggcaaatct gccgctggtg cgaattggtg
     3061 aagacgcgca ccgcaacccg ttaatgcaga acgaaaaacg taaaaatttg accatcctga
     3121 ttgtcggcga aacctcgcgg gcggagaact tctccctcaa cggctacccg cgtgaaacta
     3181 acccgcggct ggcgaaagat aacgtggtct atttccctaa taccgcatct tgcggcacgg
     3241 caacggcagt ttcagtaccg tgcatgttct cggatatgcc gcgtgagcac tacaaagaag
     3301 agctggcaca gcaccaggaa ggcgtgctgg atatcattca gcgagcgggc atcaacgtgc
     3361 tgtggaatga caacgatggc ggctgtaaag gtgcctgcga ccgcgtgcct caccagaacg
     3421 tcaccgcgct gaatctgcct gatcagtgca tcaacggcga atgctatgac gaagtgctgt
     3481 tccacgggct ggaagagtac atcaataacc tgcagggtga tggcgtgatt gtcttacaca
     3541 ccatcggcag ccacggtccg acctattaca accgctatcc gccgcagttc aggaaattta
     3601 ccccaacctg cgacaccaat gagatccaga cctgtaccaa agagcaactg gtgaacactt
     3661 acgacaacac gctggtttac gtcgactata ttgttgataa agcgattaat ctgcttaaag
     3721 aacatcagga taaatttacc accagcctgg tttatctttc tgaccacggt gaatcgttag
     3781 gtgaaaatgg catctatctg cacggtctgc cttatgccat cgccccggat agccaaaaac
     3841 aggtgccaat gctgctgtgg ctgtcggagg attatcaaaa gcggtatcag gttgaccaga
     3901 actgcctgca aaaacaggcg caaacgcaac actattcaca agacaattta ttctccacgc
     3961 tattgggatt aactggcgtt gagacgaagt attaccaggc cgcggatgat attctgcaaa
     4021 cttgcaggag agtgagtaaa tgaaaattct gattgttgaa gacgatacgc tgttattgca
     4081 gggactgatt ctggcggcgc aaaccgaagg ctacgcgtgc gatggcgtga caaccgcgcg
     4141 gatggcggaa caaagccttg aggacggtca ttacagcctg gtggtactgg atttagggtt
     4201 acccgacgaa gatggactgc attttctcgc ccgtatccgg cagaaaaaat acaccctgcc
     4261 ggtactgatc ctcaccgctc gcgatacgct gaccgacaaa atcgccgggc tggatgtcgg
     4321 tgccgatgac tatctggtga agccttttgc gctggaagag ttacacgccc gtatccgcgc
     4381 cctgctacga cgccataata atcagggcga aagtgagctg attgttggca atctgacgct
     4441 gaatatgggt cgccgtcagg tatggatggg cggtgaagag ttgattctga cgcccaaaga
     4501 atatgctctg ctgtcacggt taatgctcaa agcaggcagt ccggtacatc gggaaattct
     4561 ctacaacgac atctataact gggacaatga accctcgacc aacaccctgg aagtgcatat
     4621 ccacaatctg cgcgacaaag tgggcaaagc ccgtatccgc accgtgcgcg gctttggcta
     4681 tatgctggtc gcgaatgagg aaaactaatt gaatctgatg cgttttctgc gccgacctat
     4741 atcgctgcgc caacggctga tattgaccat cggggccatt ttgttggtgt ttgagctgat
     4801 cagcgtcttc tggctatggc atgaaagtac cgagcagatt cagctgtttg agcaggcgct
     4861 acgcgacaat cgcaacaacg atcgccacat catgcgtgag atccgcgagg cggtcgccag
     4921 cctgattgtc cccggcgtct ttatggtcag cctgacgcta tttatctgct atcaggcggt
     4981 acgccgcatc acccgcccgc tggcggagct gcaaaaagag ctggaagcgc gcaccgccga
     5041 caacttaacg cccattgcca ttcacagcgc caccctcgaa atcgaagcgg tggtttcggc
     5101 gttgaacgat ctggtcagtc gcctgaccag cacgctggat aacgaaaggt tgtttaccgc
     5161 tgacgtcgcg cacgaactgc gaacgccact ggcgggggtg cgtttgcatc tggaactgct
     5221 ggcgaaaacg catcacattg atgtagcacc gttagtggca cggcttgatc agatgatgga
     5281 gagcgtctcc cagctgctgc aactggcgcg tgccggacag tcattttctt ccggtaatta
     5341 tcaacatgta aaactgctgg aagatgtcat tctcccctcg tatgacgaac tcagtaccat
     5401 gctcgaccag cgacagcaaa ccctgctatt gccagagagc gccgccgaca tcaccgttca
     5461 gggtgatgca accctgctgc ggatgttatt gcgaaacctg gtagaaaacg cccatcgtta
     5521 cagcccgcaa ggcagcaaca ttatgattaa gctgcaagaa gatggcggag cggtcatggc
     5581 agttgaagat gaaggaccag gtattgatga gagtaaatgc ggggagttga gtaaagcgtt
     5641 tgtacgtatg gacagccgtt atggcgggat tggtctgggg ttaagtattg tcagccgcat
     5701 tacacagttg catcacgggc agtttttcct gcaaaaccgg caagagactt ccggcacgcg
     5761 ggcctgggta cggctgaaga aagatcagaa cgtggcaaac cagatataaa gaaagctgct
     5821 gaccaccagc acgctgaaca cggtagttaa actttcataa acacggtttt tcattgcact
     5881 ctcctccgtt aacctggagg agagtatgcg cgtcagagat taagtgaacc ttaagagttc
     5941 aattggcctg ataagacagc gtcacatcag gccatccgtt tcagcttatt catcaattcg
     6001 cggatgttgc tgcaccaggc gggtacgttt cgcctgcaaa tcggcaatct cgtggtcaat
     6061 attatcgatt ttctgctcga tattatcgta atgctcgacg agaatttcct tcgcttcctg
     6121 tatatctgac gccgccggtg tcgcaccttt caacggacga tttgccgtct ctttcatggt
     6181 tacgccggtg attaaaccaa tcaccgccac taccatcagg taataggcag gcatcatcag
     6241 attctgcgag ctttcgacca gccaggccgc cagtgttggc gtcagaccgg caaccagcac
     6301 cgaaatatta aatgccgccg ccagcgcgct gtaacggata tgcgtcggga acatcgctgg
     6361 caaggtagaa gccataacgc ccataaagca gttaaggatc accgccagca tcagtaaccc
     6421 ggcaaaaatc aggccgatga cgttactgtt aatcagaata aacgccggga tcgccaacac
     6481 aaacagtgca acactaccaa gtagcacaaa cggacgacgg ccaaaacggt cactcagcaa
     6541 gcccatcacc ggctggacaa acagcatacc gatcataatg gcgataataa tcagcacccc
     6601 gtggtcttcg gagtaatgca ggttatgcga caaataactc ggcatatagg tcagcaacat
     6661 gtagtaagtc acgttggtgg caattaccag accaatacat gtcaacaggc tgcgccagta
     6721 tttagtggca atctctttaa acgagacttt cgggccatcc tgcaaacctt cgcggtcgcc
     6781 ctgttccagt ttatcaacat gctgctggaa cgccggagtt tcttccagcg catggcgcag
     6841 gtaaagcccg ataatcccta acggcagagc aataaagaac ggaatacgcc agccccagtc
     6901 gaggaagttc gcttcgccga caatggtcga aattaacacc accacgcccg cacccagcac
     6961 aaacccggca atagaaccga agtccagcca gctgcccata aagccacgtt tacggtcagg
     7021 ggagtattcc gcaacaaata tcgacgcccc ggtatattca ccaccgaccg agaaaccttg
     7081 tgccatctta cagatcaaca gcagaatcgg tgcccaaata ccaatcgtgt cgtaggacgg
     7141 tattaagcca atacagaacg tactgatcga cataatcaca atagtgatag cgaggatctt
     7201 ctggcgacca tatttatcgc ccaacatacc aaagaagagt ccgccaagcg gtcgaatcag
     7261 aaagggaacg gagaaagtgg caagtgcagc aaccatctgc acgctggggt cagcccccgg
     7321 gaaaaaaact ttacctaatg cgtaagcaac aaaaccataa acaccaaaat caaaccattc
     7381 cattgcatta cccagtgatg ctgcggtaat ggctttacgc agtttaccgt catcaataat
     7441 ggtgacatca cgaagggtaa tcggttttac ttttttcctt ttcagcatag ctttcctcgc
     7501 agagttggcc catttccgca aacgccagaa aaatcagcgc gacggccgca aaaggggagc
     7561 gggaattgca ggatgcaatc tctgtaacaa gcgtagcggg tttgcttaca ccctccggtt
     7621 aaggaatgta caatcaaacc agttgacgcc tgaattgggc agttaatgac ctttttaccc
     7681 taccagtgtt taaatttgtg atcaacttca cacatggatt tcgtttagga ctcattgatg
     7741 taactgatta tttacgccaa atttacagag aagatttcct cgaccattgc ccccaccaaa
     7801 cgctgcgctg aacagagtcg tggcatcccc agcgcgacag tttgccagcg ttgggtcact
     7861 gaccagcaaa cgggttgttt atctgccgtg gtccactttc ccagccaact taaaaaatca
     7921 gcatgatccc cgcccgtcgg aacggtcgaa gaggcaaacc actgatggta atagtgacgc
     7981 gtagtcggtg ccgcattgat actctctgcc agatgttgtg tcgctttctc gcgcaaatca
     8041 cccagcaaca acaccaccgc ttgcgggctt cccagacggc aggcatcgtc aaatgcaccc
     8101 cagcgcaact cctccagaac gataaaacag cgcccaggca acgaccaggc gttatacgcc
     8161 ccagcacgcc agcgggtaaa aatttgctcg ctctgatcac ctgcctgcac cagtaggcct
     8221 cgttgcgtta acgccttttc cttccagcga caacgggctg aaaaccactc atacaaggcg
     8281 gcaatttgct gggggagctc cgggaaagca gagtgctggc ggtgatccat tttttgcagc
     8341 agcgccagat gctgcgcgat ctgcgtcagc gccagatgac cgggatccag catcgacgcc
     8401 agtttttcca ccattgtcag tcgcaggcta agcggcgcat ttaacatgcc gccgttcgtc
     8461 catgtctgaa gctgcacacg cgaaagaagt tcctgcatca gtcgttcacg aaactgctgc
     8521 tgatgggctt gaggatggcg ggcatcatcg ccctgcgcca gatccactaa aaagcgcgga
     8581 tgaaccgact ccagcacgcg accggggccg tccagtaacg tcttggtcaa tatcgttctg
     8641 ccgtgaaaag tgtttgaata tcatcgcgta acagctgagc gtcggtgtaa atccagctgt
     8701 gagtcatcac agtctgctgc aattgctgct gcatcagcct gaccaccgat tcattttgtt
     8761 gacgcagagc cagactttcg cgtaaacgcg tctgtaattc tgtcaaacat gaagcgaact
     8821 cagcgaaaaa agtattcatg cctgccgtaa cagattcatc gacctgctct gccagaactt
     8881 tagccatttg ttggcaatag agatcgactt ctgcgcttaa tgctcgttgc aacacactgt
     8941 aatcaaccgt ttctgtcggg gatttctcat ttccccgtcc ccagtcgggc tgattcaacc
     9001 agcgcgaaaa agtctcacgc acaacgccta aacgcgtgct ctgctcgtcc gttgcttcct
     9061 ggcgcgaaat gacttcactg aacagctggc gagtgttgaa gtggggaact acgccgtgaa
     9121 aaacaggaaa atgaaaccca ggacgaaacc ctgactcgct caattccatt ttgacttgtt
     9181 gctcaatggg gcgaataaca tcggttaaca cccggcaaag ggtggattcc atctcggcaa
     9241 aacgcagcgt aaagtcgcga ctgatggtgt tctgcgccgt ctgtaacagt gtctcacagc
     9301 gggtacgaat ctcgcttaac ggctccgaat catcctgaaa caaggcggct aactgcgcat
     9361 tcaccgcatc ttgctgttga cgcagaaagt ggttggcgga ggtcagggcc agctcgattt
     9421 catgtttaat ctcgccgctc acctgcgcct gattgagttg caatagctgc aaactttctt
     9481 cgacctgatg gatattttgc cgcaattgtt cacaagcgac gtttaacccg tgcgcacgaa
     9541 aatccaggta ttcccgcgcc tgctgcgcgt aattcaacag tttatgcgca gcagatcgca
     9601 gagcatacaa tgaggcgtta gcgtaagcgg catgaagcaa cgcctgaatt ggctgggcga
     9661 acagcgaatc ttcccacaac tgatcggcag catgacgaat atgttcgagg tccgccagat
     9721 cggcatgacg ccagcgcctg ccgagcgcgg catgggcaaa atcttccacc cagcgttgtt
     9781 gctctggcgc tggtaactta ccgttgttgg ctaactcata gcgcgcccga ttcgccaggt
     9841 agccccacat cgacgacacc gggaatatct gctgtggcgt aatacagcct ttcatcagcg
     9901 tcccggaaat cagtgcccgc acctggtcgg cgtcgtcact gttacgatcc tgttgatcga
     9961 acttattgac cagcacatac agcggcaccg attgccccac cgccaaaatc gcctcacgga
    10021 cctcttcatc ggagatcgat ttcagttgcg tataatccag caccgccagt accgccgagg
    10081 cgcgtgccag ctgctggtta agcatttttt gcagatgcgg ttgcccggct tcatttggcc
    10141 cgggggtatc cagtaacgtc aactgaccgg gataactttc cagccccgcc agatggacaa
    10201 actccacttc aatcacggga atatgctcaa tggcggcgta agcagaaaaa ggaaaatcga
    10261 cgtccagcgc cttcgccagt cgcactaaat cattcaaact tttcagacaa tgaaaaatag
    10321 gctgggcacc cagataatat ttttcgaaag cgacgccatt ttcgatccgc tgcataagcg
    10381 cacgcatatc tttatctatt tccagcacat cggtcagatg cttaatatcg caatcacgca
    10441 ggcgctgttg taattgttga attaaacaat cgattggcgc gacatgtgaa aaatgcagta
    10501 ccggttcctt ttgcccgggc gtatggcgaa taagcgtcgg cagcgcagtc attgggcgat
    10561 tacgattagg cagaacctcc gtaccaacaa tggcattaat ggtggttgat ttccctgctt
    10621 tcatggtacc gacaattgcc agcaccattt ccagtcggga aattttacac aactcattat
    10681 tcagcatcgc gtgacgttcg gcgatattag gctgactcca gggtaaagcc agttgtggcg
    10741 cgtcgtctcc gggtacagag agaggcattt tttccagtaa ctgcaactgt tggcgagaaa
    10801 gctgtaacag gcgttcagcc tcctgactt
//
LOCUS       DAZSNM010000139        10817 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZSNM010000139 DAZSNM010000000
VERSION     DAZSNM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.139"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..877
                     /locus_tag="ACHW42_002922"
     CDS             <1..877
                     /locus_tag="ACHW42_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8944658.1"
                     /translation="AVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRS
                     VLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSDTLS
                     LFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIVHEPLMAMI
                     PYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQ
                     EVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQS
                     PAARNFRIHLLNALR"
     gene            1012..2232
                     /gene="mlc"
                     /locus_tag="ACHW42_002923"
                     /gene_synonym="dgsA"
     CDS             1012..2232
                     /gene="mlc"
                     /locus_tag="ACHW42_002923"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR8944659.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            2357..3052
                     /gene="bioD"
                     /locus_tag="ACHW42_002924"
     CDS             2357..3052
                     /gene="bioD"
                     /locus_tag="ACHW42_002924"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR8944660.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3005..4297)
                     /gene="clcB"
                     /locus_tag="ACHW42_002925"
     CDS             complement(3005..4297)
                     /gene="clcB"
                     /locus_tag="ACHW42_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR8944661.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4456..5070)
                     /gene="dmsD"
                     /locus_tag="ACHW42_002926"
     CDS             complement(4456..5070)
                     /gene="dmsD"
                     /locus_tag="ACHW42_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR8944662.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(5113..5967)
                     /gene="ynfH"
                     /locus_tag="ACHW42_002927"
     CDS             complement(5113..5967)
                     /gene="ynfH"
                     /locus_tag="ACHW42_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR8944663.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(5969..6586)
                     /gene="ynfG"
                     /locus_tag="ACHW42_002928"
     CDS             complement(5969..6586)
                     /gene="ynfG"
                     /locus_tag="ACHW42_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR8944664.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(6597..8635)
                     /locus_tag="ACHW42_002929"
                     /pseudo
     CDS             complement(6597..8635)
                     /locus_tag="ACHW42_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(8696..9730)
                     /locus_tag="ACHW42_002930"
                     /pseudo
     CDS             complement(8696..9730)
                     /locus_tag="ACHW42_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            9788..>10817
                     /locus_tag="ACHW42_002931"
     CDS             9788..>10817
                     /locus_tag="ACHW42_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR8944665.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVE"
BASE COUNT         2671 a         2762 c         2808 g         2576 t
ORIGIN      
        1 tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata tttcgcaacc
       61 gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac tgctggcacg
      121 aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag atagtcggca
      181 aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc agggtgaagc
      241 gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg tgtccgatac
      301 gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg aaatgaacac
      361 tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc tgcgtaacac
      421 agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta tggcgatgat
      481 cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac tggctgaact
      541 ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga ctgggctgta
      601 tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca ctcaggaggt
      661 gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt caattttgcc
      721 tgcgtcattt aaacgtgttc agctcaacga aatgcgctgg gtgccgattg ctgaagagga
      781 tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc cagctgcgcg
      841 taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc gaaaaagccc
      901 gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta ttgaagtgct
      961 tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc ggtggttgct
     1021 gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc ggtttatcgc
     1081 ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc gcagctggct
     1141 cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt gcgagagctg
     1201 gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt tgaaactgaa
     1261 gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc tctgcgcgat
     1321 ctgagcagca aactggtggt ggaagagtcg caggaactgg cgttaaaaga tgacttgcca
     1381 ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca gaaaaaactt
     1441 gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga aaatggtatt
     1501 gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga ggcgctggag
     1561 cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac gatggcagag
     1621 gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat cgatcacaac
     1681 gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag tagtctcgtg
     1741 gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg gaaccacggc
     1801 tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct gcgtctcaat
     1861 caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt gtgtcaggcg
     1921 gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc gcatgtaggg
     1981 cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat tggctcaccg
     2041 ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg tcagcaggcc
     2101 cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa ccagggcacg
     2161 atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt gattcgtctg
     2221 ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca atttgggcca
     2281 ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt tgtgaagcat
     2341 aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca cttctgtagg
     2401 gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa aaagggttgc
     2461 gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc gtaataaaga
     2521 tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag ttaatcctat
     2581 cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt acaccctcat
     2641 ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag aagggactgg
     2701 cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag tgcaggaaca
     2761 actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg cactgctaac
     2821 agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta accgaatcaa
     2881 cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac ttccggcacc
     2941 gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg ggcaatacat
     3001 ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt aacgtccgcg
     3061 aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga tactccccgg
     3121 tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc gccaacagtg
     3181 tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg aaccataatc
     3241 ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc ggtgtaaaga
     3301 caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta caaaggaaga
     3361 tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg gtgctatagc
     3421 cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac ccgcctagtg
     3481 ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag gcgttcatta
     3541 acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata atcagcgcat
     3601 agtcacgagc ctgaaccgtc actgagagtt gtacgttgta gagtaacgcg tcgctatgat
     3661 taatcagatt gctaaccagc catgccacga tggcggaaat aatcaccggg ccgagagagg
     3721 ccaacatcat agtgccaaac agcacttcgg ctataaataa actgccagca agcggggcac
     3781 gataggccgc agccattccc gccgcggccc cacaggcgat ccataatttc cattcctggc
     3841 gtggcgtaaa acgttgggca aaacaggagg cggcaagggc agctaaaaga atcatcgcac
     3901 cttcgcgacc aattgcactg ccgctggtta ctaccagcag cgaggcaagc gatttaacca
     3961 ggcttgctgc gtaatcgaac tgtccatcgg tttgcaacgc ttccatgtaa tcggtcggcg
     4021 catgagggcg ttgttgggta aatttctgcc agcccatcag caacaaaccc gccgccagtc
     4081 cgccgagcgc cggagttagc agccgtcgcc aggaggaaag gtttgtcgct gcattgacca
     4141 gactgccgga gtcattattg aggaacaacc actccagtag cagcatcgca tgacgaaacc
     4201 cggcaacggc aaagacagcg agaataccga cgactgttgc gataagcaga cggcggaaca
     4261 tagtgcgcag atccggatag atgtgtagat gatgcatgag cagaaatggg caatgaaaca
     4321 cgggcgacta ttttgcggca aaagaggcca accgcaaaag cagtgcgtgc tttaagagat
     4381 aaaaaaacgt gacattgtaa cgtattattg ccgaatcgtg cgtaatcgtc accatccggc
     4441 aatattacgg tgatcctatc gaaagagtgg tttaaccgcg acaggaatca acagttgtga
     4501 ttgccattgc gccagcgtta agcgagccag ttcacccagt gcacggtaga aagggtgttc
     4561 tgctttttcg ataaaaacat caagaaaacg tgttgaccac ggaaaaaggt gccatgccag
     4621 caattcttcg cattctgtct ggcgaccatt ctccgccaac cacgcagcca tcaacagcag
     4681 tgaaccaaaa tgatcttccg gttcgttttg cttcatttca aactgaatgc ctttctcgcg
     4741 catccactga cgaagtgcca atgttgaatc gccaaacagc acagattcgc gatccagcca
     4801 gaccgacccc cacggcggag acggcagtgc ccacgggccg acaaacaaac gctgccaggc
     4861 ctgggcgtga gtctcttcac actgtgtctg aaaagccgtc accagcggcg ctaatgacgc
     4921 ttctggtaga ggccactgag tttcccagcc gtcactggtg agtaccgcaa ccagaggtgc
     4981 ggcttccgca ctctctggag cgtaataaaa cagcgcgccc aacacgcgcg ccgcgacaga
     5041 aaaattatct tgctgtgaaa aatgggtcat tcctaacatc ctgaaatgtg cgggttgccc
     5101 cgcaccaatt tgttaacctg caatagccat tcctactgtc atgtgcaggc cataaaagag
     5161 cacacgcccg atcatctcac caacaagtat gaggattagc cccagaacaa gtccggcaac
     5221 gtgaggttca cggcgacgaa tgagcggaca aagccagcat ccaagacctg cgcataacaa
     5281 taccacacgc cagacctgta aggaggcgta atctggtacc agtgcgctgg cttgttgcac
     5341 ggatgagtga atagacgcca gagacaaacc ttgcagcaca atcacctcgg cacaagcgat
     5401 cagcgccaga acgctaatga tggcaaatgg tgtggtatta aatgtaacgc gggctgcccg
     5461 cagaattgcg gcagcaagta ttggaccgct caacaatacc gtcaggaaga aagccagcgt
     5521 agtgtaaccg ttatgccagg ttggcacggt gtcgatttga tacacgcagg tcatcatcca
     5581 gacgaaaatg acgccgagcg ccatgctgac aagtaaccag agtttcccca acgcttgcgg
     5641 cattttaccg atgaccgcca ccagccacca tagtccgcca acggcaaaaa atatggaacc
     5701 ggcagcaatc tcgttactca gaccagaagc tccgattcga ttcagcgagt taaacgctcg
     5761 cagtggcgat cccaggtgca tgacagaggc aataaacccg atgcccatca acagccagag
     5821 gaaaaacatg ccacgaacaa tgtgctgacg gtctgcgtca tttttggcgg cgaaccagcc
     5881 aattccgcta acaatcagcg cgcctactac gcattgaccc aatacggtaa agataaccag
     5941 tggccattca tgccatccat ttcccatttt acacttcctc cggattagcc agataaccag
     6001 tggtatcgcc ggtcgggcgg ctgttggcgt taggtttgat aacgatattg ggttttgtga
     6061 aatgcgcgcg cggcagtggc gcgacggcag ccagagtgcc gtgcttctgg cgcagctctt
     6121 caattggacc gaactcaagt gcacgcagcg ggcaggattc gacgcatatg ggttgtttcc
     6181 cctcggcgac gcgcgaataa caaccatcgc acttcgtcat gtgccctttt tcagcattgt
     6241 actgtggtgc gccgtacgga caagccatgt ggcagtagcg gcagccgata caaacatctt
     6301 catcaaccac cacaaagcca tcttcacgct tatgcattgc gccgctgggg cagacttttg
     6361 tacatgcggg gtcatcgcaa tggttacagg agatggagag ataataagcg aacacgttct
     6421 ggtgccagat gccattatct tcctgccagt cgccgccagc atattcataa atacggcgga
     6481 aactgacttc cgggccaaga tctttaaaat ctttgcacgc cagttcgcag gttttacagc
     6541 cagtacagcg gctggaatcg ataaaaaatc catattgtgt ggtcatgggc tactccttaa
     6601 accttttcga tctggacaag attgctgtgc gacgggtttc cctttgccag cggtgaaggg
     6661 cggtgagagg tcagaatatt gatactgccg ccgtgatcga cccggtcacc aaacatatcc
     6721 gctttaagcc acgcaccttg cccgatggcg gtaacgccag gcagaatacg cggagtcact
     6781 tttgcggcaa tcagcatttc tccattattg ttaaataccc gcacggtatc gccatgacgg
     6841 ataccgcgtg cctgagcatc aatggggttg atccacacct cttgtgggca ggcctgctgt
     6901 aacacatcaa tattgccgta gctggagtgg gtacgcgctt tggtttggcg acttcactca
     6961 gcatccagta gatgggtttg cgttcaaatt ttgctgaggt tgcgggttgg gcgaggatca
     7021 catagcccat attccctgca gattcatgag agataaggtc ttcttgctct gttggcatca
     7081 ggtcaggcaa caggatatcg caatacttag ccgaggccgt catgaagtgg tcaatgccaa
     7141 caatcatctc gcacttgctg tcatcctgaa gcacctcatg ggtgtgattg atgtcgccat
     7201 gttgattgat caatgtgtta ctggcgtagc accataaaaa cttgatgggg acatccagtt
     7261 tttcttttcc acgaacacca tcacgggtcg cggtcatttt cgtaccatgg tcgatggcat
     7321 ctgtccatgt aaaaacggaa atctgcgttt taacaggatt ctcgagcatc gggaaccatt
     7381 ctacccccag atcccagcta ccttcgcgta cacctgagtt gccgccgttt atgccgacgt
     7441 taccggtaag aacggaaagc atggcaatag cgcgggacgt ttgttcgccg ttggagtgtc
     7501 gttgtggccc ccaaccctga caaatataag caggttttgc tgaaccgatc tctcgtgcca
     7561 actggataat tttttctgcc gggatgctgg tgatttttgc tgcccattcc ggcgttttag
     7621 ctatgccgtc aggcccttcg cccagaatat aggctttata atgcgcgtta cgtggtgcgt
     7681 tggcgggcag cgttttttca tcgtaaccaa cacaatattt gtcgagaaat ggctgatcga
     7741 ccatgttttc agtaatcagt acccaggcaa tcgcacaggc cagtgcgcca tcggtgccag
     7801 ggcgaatggg cagccattca tcttcacgcc cggcagctgt gtcgttatag cgtggatcga
     7861 tgacgatcat gcgtgcgttt gaacgttcgc gggcttgctc gacgtagtaa gtgacaccac
     7921 cgccgctcat ccgcgtttct gccgggttat ttccgaacat aacgaccagt ttcgtattgg
     7981 cgatatcatc cgggctgttg ccatcattgg caccgaacat ataactcatt gcggcactga
     8041 tctgtgcggt actgtagctg ccatagcgac tgagaaaacc cccgcaagag ttcatcagac
     8101 ggtacgggac gttcgagttg gtgatatttc cgccatctac gcctgttccg tacaggacat
     8161 gtacagcctc attgccgtaa tctttcagga tccgccgaag attatcactg atggtatcca
     8221 gggcttcgtc ccaacttatc cgttcaaatt taccttcacc gcgctcgccg acgcgcttca
     8281 tgggatattt caacctgtca ggatgattca tccgtcggcg gatagagcgc ccgcgtaaac
     8341 atgcccgaac ctgatgatta ccgtaaacgt cgtcacctgt cgtatcagac tccacccagt
     8401 acacggtgtc atctttcaca tgcaaacgta gcagacagcg gctcccgcag ttaacggtgc
     8461 aggaactcca gaccgttttc tcttctaccg gagcctctgc cgcccggacc atttgggaaa
     8521 atggcagagt gaaagcactg cttgccagcg cgagactgcc aagtgcggag gttttcatca
     8581 gacttctatg gctgatttca gccttcatga gcgcctctgt ggtatggatt ttcatcatta
     8641 ctcacttatt gcttttcaaa caaaatgtca tgccagaatt tatggttgta gtgggttata
     8701 ttttttcgat ctcgaccaga ttagtgtgct gcgggtttcc cttcgccagt ggtgaagggc
     8761 gcagagtggt tagcgtattc acacatccgc catggtcgat tttatcgcca gacatattgg
     8821 gctcgtgcca ggctccctgg cccatagcgc taactccagg gagaatgcgt ggtgttactt
     8881 tggctggtag ccgaacttcg ccacgatggt taaacacccg caccatatcg ccgttggcaa
     8941 tcccacgttt ctgtgcatct atagggttga tccacacctc ctgacggcag gctgccttca
     9001 ggacatcaat gttgccgtag gtcgagtgag tacgggattt gtaatggaaa ccaaacagtt
     9061 gcagtgggaa ggttctacgt tcaggggagt cccagccttc aaaggttgag gcataaactg
     9121 gcagtgggct tatcacttca tctttttcca gttcccaggt acgggcaatt tccgccagct
     9181 tgctggaata aatttcaatc ttaccggaag gcgttttaag tggatttgcc tcggggtcgt
     9241 cacgaaatgc tttgtaggcg acaaaatggc cattgggatc tttacgctta tagataccca
     9301 tttttttcag ttcgtcgtaa gacggtaacg ccggatcttt ggcaagcatt ttggcgtaca
     9361 gatgttgtaa ccattgttcc tgcgtgcgac cttctgtgaa cttttgatag acgtcaggtc
     9421 caagacgttt cgcgacttca ctcaggatcc agtaaatcgg tttacgttcg aatttttcgc
     9481 tggtgactgg ctggaggaaa atgagatatc ccatgttacc ggcgtagtcg ttaggaataa
     9541 tatcttcctg ctcaacggtc atcaggtctt ccagcagaat gtcggcatat tttgccgatg
     9601 aggtcataaa gttttcgatg accacaatca tttcgcattt cgattcgtcc tgcagaattt
     9661 catgcgtttt gttgatgtca gaatgctgat taacgagggt atttcccgcg tagttccaga
     9721 tggactccat tgccagacgg atggatggac ttgaagtagt tgactcaatg ctacttaagg
     9781 tcctgatatg actgacatta atcatgataa cactgtatat ccatccagtt ctttctctgg
     9841 gtctccctat cggtatcagc aagtctacac ctcaagccct gacacaaggc tgacacaaat
     9901 caatctactc agcgtagcta agcctcagtt agtccgcaga gccaatggtc gatatactat
     9961 ccgcttcaga ctcaagggac agaccacacc gtttctttca gtatcgaccc gcagcacaga
    10021 caggagagta gccacaatgc gtcagagaga gcttgcagcc accgctaagg ccttcatgtt
    10081 ggataggcct gaagtctcac tacaggagct tacagagcat ctccggtcta tggctgagca
    10141 gttcctgaca gacgctagtg acgattactg gaatggactt gaggtcgcta ctctggtcga
    10201 cgagaagtcc aacctgaagg agctagccgc tacgcaagcc ttgagtctgg accagcagaa
    10261 gggcataagg ttagcgctgg aagtcctgac cgcagcacag cagagagtcg acactggtga
    10321 tacctctggt ctgattaagc tgattgatga taataacctt actgatgact caactatagg
    10381 cgactctacg tcaattctga ataatgagca gggggataga ccggcagttt tcacacagga
    10441 gcgccagtca tctgtagttt tttcaagcct agtgtcctcg ctgctggctg agaaggtcca
    10501 gaccctgaag acatccagct acaaggacct ctcgtcctca ctcaatactg tctcccggtt
    10561 cctgcctgaa gacatggacc tgatgtcccg ctctgggtgg ctggctgtca gggactctat
    10621 gctggcttct gaggtgagac catcgacaat caacaagctg ctgaccaaag cgaagatgtg
    10681 tctcgattat ggcctgatga atgggcaact tgagggccgc aaccctattg aacggatgaa
    10741 gctgaccaag gatatcgact ccaagcgcag agcgttcaca gacgaggagc tggagagact
    10801 tctggtccgt gttgagt
//
LOCUS       DAZSNM010000140        10660 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZSNM010000140 DAZSNM010000000
VERSION     DAZSNM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10660
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.140"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACHW42_002932"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACHW42_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR8944666.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="ACHW42_002933"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="ACHW42_002933"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFR8944667.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="ACHW42_002934"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="ACHW42_002934"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR8944668.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="ACHW42_002935"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="ACHW42_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR8944669.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="ACHW42_002936"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="ACHW42_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR8944670.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="ACHW42_002937"
     CDS             4025..4615
                     /locus_tag="ACHW42_002937"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR8944671.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="ACHW42_002938"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="ACHW42_002938"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR8944672.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="ACHW42_002939"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="ACHW42_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR8944673.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="ACHW42_002940"
     CDS             complement(6551..6658)
                     /locus_tag="ACHW42_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFR8944674.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="ACHW42_002941"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="ACHW42_002941"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR8944675.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="ACHW42_002942"
     CDS             7648..7875
                     /locus_tag="ACHW42_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR8944676.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACHW42_002943"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACHW42_002943"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR8944677.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10658)
                     /locus_tag="ACHW42_002944"
     CDS             complement(9447..10658)
                     /locus_tag="ACHW42_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HFR8944678.1"
                     /translation="MKQAWNDIKNEYEKNQTYSITLFENTLVCFMRLYNELRRKVNEE
                     DTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSGGITYTIQYVQDVDIV
                     RVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLDNENMSIQPEQINMPG
                     ISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAILVLKEHEYSWALGSHK
                     TQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQSDNKIQGVGSFMSWSF
                     LFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRIKAVKNFLAVLSDSIC
                     SLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPPELFSQAYKLKNKEMN
                     AQLDYLNRYAT"
BASE COUNT         2513 a         2728 c         2686 g         2733 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc attatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 gactctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat
//
LOCUS       DAZSNM010000141        10608 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZSNM010000141 DAZSNM010000000
VERSION     DAZSNM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10608
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.141"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..801
                     /locus_tag="ACHW42_002945"
     CDS             <1..801
                     /locus_tag="ACHW42_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR8944679.1"
                     /translation="NQFNTNNHFLHHPKVDSELTRRYEYARLDTENIYLLPLARGNNH
                     NYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKITSALDEIYGG
                     ICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYAGTTIGYISTL
                     PASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGNDPIIGIEAYP
                     GTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(942..1715)
                     /gene="uxuR"
                     /locus_tag="ACHW42_002946"
     CDS             complement(942..1715)
                     /gene="uxuR"
                     /locus_tag="ACHW42_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR8944680.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1930..3390)
                     /locus_tag="ACHW42_002947"
     CDS             complement(1930..3390)
                     /locus_tag="ACHW42_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR8944681.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3471..4655)
                     /gene="uxuA"
                     /locus_tag="ACHW42_002948"
     CDS             complement(3471..4655)
                     /gene="uxuA"
                     /locus_tag="ACHW42_002948"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR8944682.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            4995..6338
                     /gene="gntP"
                     /locus_tag="ACHW42_002949"
     CDS             4995..6338
                     /gene="gntP"
                     /locus_tag="ACHW42_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR8944683.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6589..7491)
                     /gene="fimH"
                     /locus_tag="ACHW42_002950"
     CDS             complement(6589..7491)
                     /gene="fimH"
                     /locus_tag="ACHW42_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR8944684.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7511..7954)
                     /locus_tag="ACHW42_002951"
     CDS             complement(7511..7954)
                     /locus_tag="ACHW42_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8944685.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8027..8557)
                     /gene="fimF"
                     /locus_tag="ACHW42_002952"
     CDS             complement(8027..8557)
                     /gene="fimF"
                     /locus_tag="ACHW42_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR8944686.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8567..>10608)
                     /gene="fimD"
                     /locus_tag="ACHW42_002953"
     CDS             complement(8567..>10608)
                     /gene="fimD"
                     /locus_tag="ACHW42_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR8944687.1"
                     /translation="IGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKW
                     QHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVI
                     HGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFT
                     VPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLA
                     DRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQ
                     LVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTV
                     TQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRD
                     QMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSY
                     SVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANG
                     VTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTL
                     ADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSS
                     GIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2527 a         2757 c         2630 g         2694 t
ORIGIN      
        1 aaccaattca atacaaataa tcacttcctt catcatccaa aagtcgacag tgagttaaca
       61 cgtcgatatg aatatgcacg cctggatacg gaaaatatct atcttctgcc tcttgctcgt
      121 ggcaataacc ataattacga tggaaaaagc gttgtcgaaa ttagaaaact taatattggc
      181 aaacagtcct ggccatttaa ttatgtgaca gatacatgca gagaattcaa tggtattaca
      241 acaacaggac gtatgctcta tcgaaacttg aaaatcacct cagctttaga cgaaatatat
      301 ggtggtatat gtaaaaaggc gcatgctgct acagagttag cagaaggttt acggctaaat
      361 ttatttatga agagcccttt cgatcctgtt gaggactata cagttcatga aataacatta
      421 ggccctggtt gcaatgttcc tggttatgcc ggaaccacga taggatatat ttcaaccctt
      481 ccagcttctc aggcaaaaag atggaccaat gagcagccaa gaattgatat ctatattgat
      541 cagataataa ctgtctcagg ggtagcaaat tcttccggat tcgcccttgc cgcattactt
      601 aatgctaata ttgagatggg aaatgaccca atcattggta tagaagccta tcctggtacc
      661 gcggagatcc attcaaaaat gggatataac gttatccccg gtgatgaaga tgcgccactc
      721 aaacgcatga ctctgcaacc gtcatctctt ccagaactgt ttgaattaaa aaatggtgaa
      781 tggaattata ttggtaaata gccactgaga agaatgagta ctaatattca ggttatacag
      841 atatttaaac aaaaccctgc aatttacaaa gtaatactca ttgcgacttc ttaatttaag
      901 aaagcgcaag gaacgtttac ccttgcgctt attataataa gtcaggcgtc gactttatcc
      961 agcggccatg aatcaaagag atagccgtca aaataaatat cgtcaacgtt cgagaattcc
     1021 agcagacgtt gcttaacgtt ttccagatgc tgccacattg ccagcttcgc cgctcgggca
     1081 tcttttttga ttaacgcggc gaggatctgt ttgtgatcgc ccaaccactc tttgcgatac
     1141 aggctgtcat ccagatggct gtgcaactgg atccacattg gattgttttc ccgccactgc
     1201 caggactgac ggaataactc caccagcatg ctgttatgcg ttgcttcagc aatggcgaga
     1261 tggaactgca tgtcaccgct ttcgctgcta cccggcgcgc tggaagccag ttcacgctct
     1321 tccagttgca atgcctgacg cattttgacg atatcttcgc gggtagcctg cagtgcggca
     1381 aactcggcga tgttactttc cagtaactga cgcgcctgta acagctcaaa aggacccgca
     1441 tcattgcaga cgttggcatc cggactgtct gtgttgtggc tgcctgagct atcaagcaca
     1501 tagataccgg caccccggcg cacttccacc agccctttga tctccagcat gatcagcgct
     1561 tcacgtacca ccgtccgcgt gacatcaagc atttctgcaa tttcacgctc cggcggcagc
     1621 cgttcgccag gattgtacgg cgtctttatg atcagatcgt ggatcatcgc cccgacttcc
     1681 tggtaaggtc tttgcgcaga ggtgacagat ttcatggtgt tgtgtccgtt aggtgcggtt
     1741 aatcaaattt ttgccggatg cggcgtgaac gccttatccg gcctacaacg ggcaatttta
     1801 cggcccccgt taatctcaaa ctataccatt agcactggaa gttggcacgt acaggtgggc
     1861 aaaaaaacga aaactggtca accaaatatc agtttgattg accagtaagt ctgtagggta
     1921 atcagttagt tacagcgcag ccacacactc gcgcgcaccg cgttcgcaca gctgctgata
     1981 tgccgacgtc accgcgccaa caaagtcggc attctgcggc agatcatcgg caaaaatacc
     2041 gctcaggccc agcagcgctt tcacgcggtc tgcgccctga tactgcgcgt taatcttctg
     2101 gaactcggcc agcatcgggt cgaccacgtc aatggcatta ccctgctcat ccacgccctg
     2161 ggtgtaaccc atccagccag ccacgcccag cgccaggtga cgccagctgc cgccgttttg
     2221 caggtgcaga cgcaccgggt ccagtagacg ctgcggtaac ttctggctgc cgtccatcgc
     2281 aatctgccag gtacggtgac gcagagacgg gttgctgaaa cgctcgatca gcagcgtcgc
     2341 ataggcgttc aggtccgtac cttccggcat cgacagcgtt ggcgcttgtt cctgcatcat
     2401 cagggcaaag gccgctttgc gataatccgg gttagtcatg gtgtcggcaa tggtttcata
     2461 gccgccgagg taaccgaggt acgccaggaa agagtggctg ccgttcagca tacgcagctt
     2521 catcatttcg aacggcacaa cgtctgcaac gaactgtgtg cccactttat cccagtccgg
     2581 gcgaccatta acgaagttat cttcaatcac ccactgacgg aacggttcgc aggcaatggc
     2641 gcacgggtcg taaacaccca gctggtcagc aatttcctgt aaggtttctg gcgtcgccgc
     2701 cggaacgatg cggtcaacca tggtgcacgg gaaggtgacg ttctcttcaa tccatgccgc
     2761 tagctgcggg tcacgcgcct gagccagccc cagtaccgcg accttcgcca catggccgtt
     2821 ttcacgcacg ttatcgcagg acatcaccgt aaatgctttc agcccttttt cacgacgcag
     2881 acgcaaggct tcgacgatgt aaccaatcgc ggacttcggc gcagtcgggt tttccagatc
     2941 gtgcttgatc agcgggttat tgagatccag ttgaccgctt gccgcatcag cgcagtagcc
     3001 tttttccgtg accgttagag agacaatcgc cgtttgcgga cgcgccatcg cgttgagaat
     3061 accttcgcag ccgtcgattt ccggatgcag cgcttctttc atcgaaccga taattttcag
     3121 ctcggtgctt tctgcgcctt tttccgctac ggtgtacagc agttgctgtt ttttcaggtt
     3181 ttcgatcagc atgcggtcgt tgcctggcat caggttaact tcgcagatgc cccagtcgct
     3241 gtcggtgctt tccagcagat gatgggtata cagcgcctgg tgcgcgcggt gaaacgcccc
     3301 gcaaccgaga tgcacaatgc gtgattccag acgagaatga tcccatgacg ggcgggcaac
     3361 cggcagattg ctgtcaacaa tagtagtcat cgtaaactcc agaatccgtc ttcagagagt
     3421 gacggcaagg aagaaggaac cggcacggcg cgcagccatg ccggtgggta ttaacggata
     3481 aagaaagcgc gctggatcgc cagttcgaca ccgcgaactt cggccaggcc tttcagacga
     3541 ccaattgcgg agtaaccagg gttggttttc ttcttcaggt cgtccagcat ctgatgaccg
     3601 tggtccggac gcatcgggat caggtcttct ttgccttccg ctttacgacg gtgttcttct
     3661 tcaacaatcg ctttcaccac tttgtacata tcaacgtcac cgttcaggtg cgccgcttcg
     3721 tggaaggttt tcgggttatc ttcacgcatg gtggagcgca gatgggtgaa gtaaatacgc
     3781 ggaccgaact gcttgatcat atcaaccaga tcgttgtcag cacgcacgcc gtaggaaccg
     3841 gtgcacatgg tgaaaccgtt cgccatgctg tttacggtat caaccatcca ctgcatatct
     3901 tcaatggtgg aaacaatgcg cggcaagccg aggatcgggc gcggcggatc gtccgggtga
     3961 accgccatac gcacgccaac ttcttcagca actggaataa tcgctttcag gaagacggca
     4021 aagttttcgc gcagtttggc tttgtcgata tctttgtaca gctccaggtg cttacggaac
     4081 tggtcgaggg tatacccttc ttccgcgccc ggcagaccgg caatgatgtt acgcgtcaga
     4141 cgcgctttgt cttcgtcgct catagtggcg aagcgttcag ctgcctgagc aatttcttct
     4201 tcggtgtaat ccgcttccgc gcctggacgt ttcaggatat gcatttcgaa tgcagcgaat
     4261 tcgatctggt cgaagcgcag agctttggag ccgtctggca gcacgtattc gaggtcagta
     4321 cgggtccagt cgagcaccgg catgaagttg tagcacacgg tgcgaatgcc acactgcgcc
     4381 aggttgcgca gagtctgctg atagttagcg atccactgct catagttgcc agtgtgggtt
     4441 ttgatatctt cgtgaattgg tacgctttct acgacagacc acaccaggcc tgcgtcttca
     4501 acgatcgcct tgcgtttgag gatctcttct acggaccata cttcgccgtt cgggatatgg
     4561 tgcagcgcgg taaccacgcc agttgcgccc gcctgacgga catcagctaa agaaaccgga
     4621 tcgtttgggc cgtaccagcg ccaggtctgt tccatgattt ttcctctttc acatagtggg
     4681 ttccacagca aagagacgcg gaaccttgga gttgtttagg tatccggatg aacaaccaga
     4741 ccgcagccgt ggcgttgcat cggatgagat ggcgtataag ttctaccttt tggtaagaca
     4801 tgtcaaccag aattctgaaa ttggttaacc gcgtcgcaag aatttcactc tcatggttgt
     4861 tgctgcaaat tgcagcaaag ggtaatcgat ggttattgat cagaggatgt gacattcatc
     4921 gcaacaatgg ttgaccaatt tacataacat gtcgcccaaa ataacaacgg ttcaaccaag
     4981 taaggtaaaa cgttatgcat gtgcttaaca ttctctgggt ggtattcggc attggcctga
     5041 tgctgatact gaatttgaag ttcaaaatca attcaatggt ggctttgttg gtggcggcgc
     5101 tgtccgtcgg gatgctggca ggcatggatt tgatgtcgct gctgcacacc atgaaagcgg
     5161 gcttcggcaa cacgctggga gaactggcta tcatcgtggt gttcggtgcg gtcatcggta
     5221 aattgatggt cgactccggc gcggctcacc agatagcaca tacgctgctg gcgcgtctcg
     5281 gtctgcgcta tgtacagctg tcggtgatta tcatcggcct tattttcggt ctggcgatgt
     5341 tctatgaagt ggcctttatc atgttagcgc cgctggttat tgttattgcc gccgaagcta
     5401 aaattccgtt cctgaaactg gcgatcccgg cagtagcagc tgccactacc gcacattcac
     5461 tgttcccacc gcagccgggt ccggtggcgc tggtgaatgc ttatggcgcg gatatgggga
     5521 tggtttatat ctatggcgta ctggtgacga tcccaagtgt aatctgcgca ggtctgatcc
     5581 tgccgaagtt cctcggcaat cttgagcgcc caacgccatc attcctgaaa gcagatcaac
     5641 cggtagatat gaataatctg ccctctttcg gcgtttcgat tctggtgccg ctgatcccgg
     5701 cgatcattat gatctctacc accatcgcca atatctggct ggtaaaagat acccctgcct
     5761 gggaagtggt taactttatt ggttcctcgc cgattgcgat gtttattgcg atggtggttg
     5821 cattcgtact ctttggcacc gcgcgtggtc atgacatgca gtgggtgatg aacgcttttg
     5881 aaagcgcggt gaagagtatt gcaatggtga ttctgatcat cggtgcgggt ggcgtgctga
     5941 agcagaccat catcgacacc ggcattggcg acaccatcgg catgttgatg tcccacggca
     6001 atatctcgcc ctacattatg gcatggctga tcactgtgct aattcgtctg gcgacgggtc
     6061 agggtgtcgt ttcggcgatg accgccgccg ggattatcag tgctgcaatc cttgatccag
     6121 caaccggtca gctggttggc gtgaatccgg cgctgctggt actggcgacg gctgcgggtt
     6181 ccaacaccct cacccacatt aatgatgcat ctttctggtt gttcaaaggt tactttgacc
     6241 tgtcagtaaa agacacgttg aaaacctggg gtctgctgga gctggtcaac tccgtggttg
     6301 ggctgattat tgtgctgatt attagcatgg tagcgtaaaa gaacaccaga gcctgccaat
     6361 ggcaggctca gactgatgac taaagtctaa aaatgtgccc ggacagtccc ctcgcccctc
     6421 cggggagagg gttagggtga ggggaacaga ccagtaaagt gcgctttgtt tatgccagat
     6481 gcgacgctga cgcgtcttat ctggcctaca aagggctaac gtgcaggttt tgtaggtctg
     6541 atagcgtagc gcctcaggta ccagcattag caatgcgctg taattttttt attgataaac
     6601 aaaagtcacg ccaataatcg attgcacatt ccctgcagtc acctgccctc cggtacgtgc
     6661 gtaatttgcc gttaatccca gacttaccgc cgaagttcct actgctccta acgataccgt
     6721 gttattcgct ggaataatcg taccgttgcg cgtcaactgt acgccgacgc cctgcgctgg
     6781 tgaaaacgac gcggtattgg tgaaaatcga gttgcccgca tctgcggttg tgccggagag
     6841 gtaatacccc aggttttggc ttttcgcaca ataaacggta agaggaattg gcactgaacc
     6901 agggtagtcc ggcagagtaa cggtgacatc acgagcagaa acatcacaac cgccagtggg
     6961 caccaccaca tcattattgg cgtaaatatt ccacacaaac tgaaaatcat cgctgttata
     7021 gttgttggtc tgtcgcaaaa taagcacggc aattaatgaa ccagctttaa tagccacccc
     7081 gcccgcactg ctcacaggcg tcaaataaag cgccaccggc cacggcttat ccgttctcga
     7141 attataaaca acccgcggcg tttcgctggt ggtcggaaat ggatagctac tgccactata
     7201 ttttacggtc ccggaaaaat tagataacac gccgccgtaa gccgagcctc gttgcagtgt
     7261 gacatagtct gtaatggttt ccggataatc gttatggcaa aagatttgcg tcgaaagatc
     7321 tacgaccagg ttttgcccca cattcacgac aggcgcaagg tttacataaa cattagcgct
     7381 gccaccgcca atagggatag cggtaccatt ggcggtttta caggcgaatg accaggcatt
     7441 taccgaccag cccatcagca gtacagcaaa cagggtaata gctcgtttca ttacaattat
     7501 ctcttcgggt tcagctgtag gtataggtga tgctaatcac tgcctgaatg gttccctgag
     7561 tggctccgcc atttaccgtc aatgctctga cctgtaacgg gaagtgcgct gattgtgagg
     7621 aatcatccac ctgaactgtt ttggttgcgc cagtattcaa tgtgttgcca ctgtcatcct
     7681 gtagctctaa ctggatgttt tgcgcggtcc cctggttttt ataatatccg gtgctgtcgg
     7741 ctgccccgct gaagctggca gtgaccctcg acgttcccac cggacaatta gtcaactcaa
     7801 gcgcaacatc atgccaggcc gatgccgccc cggcagacat aagactgaaa gaataaagat
     7861 cgccgagatc taccgtggca ttggtggtgg aaaccgtaca cggtttggcg acgaccttac
     7921 cgttcaccgt gatggtgaca tcggctgcct gtatcgtcgc acttgcgagc gccaacattg
     7981 ccgccaatac atacccacgt ttgcaccatt tcatgagcat ctccagttac tgatattcaa
     8041 gagtgaaggt agccgtagca ttgatatgcc ccgcagtgac aggcacctgt gtcgccatta
     8101 gccgggcgta aaaattcagc gtatttggtt tacccggcgt cagggtcgtc cacgaaatcg
     8161 cagacgatgg agcattaagg ggtatttgat tttgctgctc attcagaagc tgtattccca
     8221 gtcccgcagc cgctgacacc gtattttcaa gtgcaagcag gttggcattg tggctatctg
     8281 caacgccggt aaacccaact tttacggcag aaacggcatt accacagggt gacagcaaaa
     8341 tacgaaatgg aacgacagga gtcgtcgcgc caatgttgtt aaattgcttc gccgcgtttt
     8401 ccatcagatc aacagtaaaa ttggttgatt cagcggccac actacagccg ttatctctga
     8461 catagccgcg gatagtaatc gtgctatccg cagccaaaac gcgacttacc gccagccaca
     8521 aaaaagcgca cagaagataa aaaggtttgt ttctcatcac gcccccttaa cgacattcag
     8581 ctgatagctg ggttaataac tgctgctgac tctctggtgg cagttgataa ttggcgacac
     8641 agtgagcatt ttcctcttct ccccatttca cctgaacttt tcccgctaaa ggcattccgc
     8701 tgaggtaaac ctgaccatta tccgcaacaa tgccgctact ctggctactc tctgatgtca
     8761 ccatcgcccc aaacggcagc ggcttattat tgtgagttag cgtcatgagc agttttatcc
     8821 caacgcgcgc tttaaactct gctcgcacga tcgccccacg agtgggaaca acgttagcga
     8881 ccgcgttatc taaatcgacg ttatcagcca aggtattggt atccagcgcc actctatttt
     8941 cccgatattc agtggcataa ggcagcacgg cataaccacg ccagtcggta cgcacccccg
     9001 tctggttttc gacttttgca tcttttgcgc caggcgcttt aacaagcacc accgtatcgt
     9061 ttaacggctg ccccagcgtt acgccattgg catgagccaa taccccaccg ctgactccgt
     9121 aatagagctg cttaatatca tcgctatggc tgtaaccgat attggcattg ccgtaaccac
     9181 cgcgataatt cagcgtggcg tagcctgtac ttccgctatt accatcgcct cccccggcat
     9241 agccggtttg cacgctataa ctgaggttgt tgtcttccag caacgtaccg tatacaccag
     9301 ccagattggt catccgaccg ttgagatcgt gtgacatgct gtagctggca ctggcatgtc
     9361 gccactgaga tttactgtca gaacgcagcc agtggctgaa aggaatattg acgttaagcg
     9421 ctaacatctg atcccgtcct ttttgccagg cgtttttcgt caggctatag ctgagcgtcc
     9481 agttgatatc ttcgaacgca gtatttaatc cagcctggaa ttgctcatcg acattactcg
     9541 ttccccaata agtttgatgg ctaccactca aatacagtgt tgatgtgcgc ccgagttgct
     9601 gagtaacggt gagctgtaat ttcccgcgtt tgttataagc gaggttgtaa tagtcggtga
     9661 atttcggctt aacctgaata actccatcct gtgtttcgat gttgtagcca ttcattcgac
     9721 tgtatgttgt atcagcgaaa ttaaaatatc cgctggtcga ataacggtaa cccactaact
     9781 gaatattcgt gcctgattca ttgagcgatt tgttatagag aaaacgcacc gattgtccgt
     9841 catgctgact gtcatcggga agtgtggaat tagcctgcgt catatccaca gacagagcgc
     9901 ccagtgcctc catgtttttc ccgataccga aattaaaagc acgataacga tccgccagtt
     9961 gcgttccacc atatattgtc cagccagccg gaaggccgtg gagtaatgta ctctggaaaa
    10021 agcggggttt ttcctgctgc gcatttccac tacggtattc tcctgccgta atggaataac
    10081 gagtatgccc ttcacgttgc aaaagcggga ctgacgaata gggtacggta aaaatctgcg
    10141 tgctgccgtc agcctctttg atcgttacct gcaagtcacc actattacct gcggcataga
    10201 tatcgttgat ggtaaaaggc cccggtggca ccgtactatt ataaatgcca tatccatttt
    10261 gtttaatagt gacctgtgca gtaccacgag caataccgtg gatcaccggg gcaaatcctc
    10321 tttggctatc gggtaacata ttgtcatctg aggccaattg tgcgccgcga aagttaatac
    10381 catcgaaaat atcgccctgg gtataaccat cacccaacgt cagccgggaa cgtaaaggaa
    10441 ttatgtctcg ctcaatccag gtaatgatat gctgccattt atttttgcta cctgatgaac
    10501 tgtcgctact gttataactc caggtggtat tgtcgcgtaa acgccacgca ccaatattta
    10561 acccactctg taggtttaaa tatgcataat ggctgttacc cccaatcc
//
LOCUS       DAZSNM010000142        10597 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZSNM010000142 DAZSNM010000000
VERSION     DAZSNM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10597
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.142"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(73..1062)
                     /gene="araF"
                     /locus_tag="ACHW42_002954"
                     /pseudo
     CDS             complement(73..1062)
                     /gene="araF"
                     /locus_tag="ACHW42_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            1859..2362
                     /locus_tag="ACHW42_002955"
     CDS             1859..2362
                     /locus_tag="ACHW42_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR8944688.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(2442..2693)
                     /gene="yecJ"
                     /locus_tag="ACHW42_002956"
     CDS             complement(2442..2693)
                     /gene="yecJ"
                     /locus_tag="ACHW42_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR8944689.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(2808..2894)
                     /gene="azuC"
                     /locus_tag="ACHW42_002957"
     CDS             complement(2808..2894)
                     /gene="azuC"
                     /locus_tag="ACHW42_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR8944690.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            3157..3480
                     /locus_tag="ACHW42_002958"
     CDS             3157..3480
                     /locus_tag="ACHW42_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR8944691.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            3651..4148
                     /gene="ftnA"
                     /locus_tag="ACHW42_002959"
     CDS             3651..4148
                     /gene="ftnA"
                     /locus_tag="ACHW42_002959"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR8944692.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(4185..4424)
                     /locus_tag="ACHW42_002960"
     CDS             complement(4185..4424)
                     /locus_tag="ACHW42_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR8944693.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            4614..5825
                     /gene="tyrP"
                     /locus_tag="ACHW42_002961"
     CDS             4614..5825
                     /gene="tyrP"
                     /locus_tag="ACHW42_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR8944694.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(5887..6552)
                     /gene="yecA"
                     /locus_tag="ACHW42_002962"
     CDS             complement(5887..6552)
                     /gene="yecA"
                     /locus_tag="ACHW42_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR8944695.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(6748..6834)
                     /locus_tag="ACHW42_002963"
     tRNA            complement(6748..6834)
                     /locus_tag="ACHW42_002963"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6798..6800),aa:Leu,seq:taa)
     gene            complement(6847..6920)
                     /locus_tag="ACHW42_002964"
     tRNA            complement(6847..6920)
                     /locus_tag="ACHW42_002964"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6886..6888),aa:Cys,seq:gca)
     gene            complement(6975..7050)
                     /locus_tag="ACHW42_002965"
     tRNA            complement(6975..7050)
                     /locus_tag="ACHW42_002965"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7015..7017),aa:Gly,seq:gcc)
     gene            complement(7202..7750)
                     /gene="pgsA"
                     /locus_tag="ACHW42_002966"
     CDS             complement(7202..7750)
                     /gene="pgsA"
                     /locus_tag="ACHW42_002966"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR8944696.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(7807..9639)
                     /gene="uvrC"
                     /locus_tag="ACHW42_002967"
     CDS             complement(7807..9639)
                     /gene="uvrC"
                     /locus_tag="ACHW42_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR8944697.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(9636..10292)
                     /gene="uvrY"
                     /locus_tag="ACHW42_002968"
     CDS             complement(9636..10292)
                     /gene="uvrY"
                     /locus_tag="ACHW42_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR8944698.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            10434..10505
                     /locus_tag="ACHW42_002969"
                     /pseudo
     CDS             10434..10505
                     /locus_tag="ACHW42_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT         2669 a         2657 c         2404 g         2867 t
ORIGIN      
        1 cataacgtct ccgtgaatca ctggtcgttc acaccccctc tgcatcatgc agaggggaat
       61 ttttccggca aattacttac cgcctaaacc ttttttctcc agttcttctt taaagttgtc
      121 acgcgtgatc agtaccacgt ctgtaacttc ggtaaatttt ggcgtttcaa cgtcttttgc
      181 tacccagttg taaagcattt cgctggattt atagccatgt acgtccgggc ttggcagcag
      241 ggaaccgtag aagccggttg cctgtgcttt agacagttcg cttactgcat ccacaccgtt
      301 aataccaata ccgatgatat cggctgcttt aaagccctga ccttcggttg cacgtacgcc
      361 gccaagtacg gtgctgtcgt tcataccgac gatcagccaa tgtttaactt ccggatgttg
      421 aaccagcatt gagttggcag cgtcaaatgc ccccgggatg tcgttagatt tggtaggtac
      481 ctgataaatt tgtttttccg ggaatcctgc agctttcagt gcgtccatag aacccgttgt
      541 acggcggcgg gcggtatcca gttcgttggc ggtaatcgcc atcactgcgc tttctttgac
      601 atcccagcca cgtttctgca tctctttata cagttcctag ccctgacgtt cgccaatttt
      661 agtcgccgcc atcatcacca gaggaacggt atccattggc ttacctttgg cgttaacaaa
      721 ctggtcatcc actgcaatga ctttcatatc gtagccacgc gctttcgcga cgatggcaga
      781 gccgagtttc gggtccggag tacaaataac gaaacctttt gcgccactgg ctgccaggct
      841 gtcgatcgcg ttcaatgttt tttcgccatc cggcacggca atcttaataa cctcaaaccc
      901 taaatccttc ccggctttat cggcaaactt ccattcggtc tggaaccacg gctcttccgg
      961 ttgcttcacc agaaaaccga gcttcaggtt ctccgccata gcggattgtg acataacggc
     1021 tgccagacca atggctgcca gggctttagt aaatttgtgc atggttctct ccagctttag
     1081 tgtcgttttg tgtagggcaa aaacgaatga cattcgttaa attaatcgga aaacaaagca
     1141 ttacctttta tctaaaagat aagtgaccgt gttgacatag ttttagcgag aaattaattc
     1201 tccataggag agcaatatca catcgcagaa ttacagtgag aacgtgcata aatttagcgg
     1261 gaaaagacat aaggaaaagc caatttgtca gacaaattgt tgaatgcaca gcagattaat
     1321 ccataagatt agcctggaaa tccttgttgt cttcggtacc catgcgggat gtcttctttt
     1381 taaccagtca ataggccgca ttacctggcg ttgagttttt gaaatggtgt aataaccaca
     1441 actcaaagat gtggaaaatg cgcgtcattc atttcgccat taattatcac tgtgctcatt
     1501 aattaacaga acacgtataa tgagagccat ctcgcaaaaa tgaaaaaacg ttttataaaa
     1561 tcatcacttc atcatgaatt caaattcatc gattaatatc aacaagatac aaaaagcact
     1621 atcattaaaa ttcattgcat ttacattgat ttcaccaatg aaatgtaaaa atatataaac
     1681 ttgatgattt aagcattttc ttataaccgt tcagacgtta ttcttatttc agatcatcgt
     1741 cagaattgac tccacgatca catttcggac cggcagaaag gaattattct gcaaacagta
     1801 attatggtgt tttgatttat cttgcacctc tccacttctg gatataagga tattaggtat
     1861 ggcaaccgct ggaatgcttc tcaaactcaa ctctcaaatg aaccgcgagt tttacgcatc
     1921 caatctctac cttcacctga gtaactggtg ttctgaacag agtctgaacg gcaccgccac
     1981 tttccttcgc gcccaggcac agagtaatgt gacccaaatg atgcgcatgt ttaactttat
     2041 gaagagtgtc ggcgctaccc ccatcgttaa agccattgat gttccaggtg aaaaactgaa
     2101 ctctctggaa gaactgttcc aaaaaacgat ggaagaatac gagcaacgtt caagcactct
     2161 ggcgcagtta gccgatgaag cgaaagaact gaatgatgat tcaaccgtca atttcctgcg
     2221 cgatctggaa aaagaacagc agcatgatgg cctgttgctg caaaccattc ttgatgaagt
     2281 gcgcagtgcg aaacttgcgg gtatgtgccc tgtgcagacc gaccaacatg ttctgaatgt
     2341 cgtgtcacac cagctgcatt gatcatacat cggcgctaat acattgcgcc gatgaagttt
     2401 ttgagaaacc gctgcctcat ctgtttgaag cagcggtttt tttaatggga ttcaccctgt
     2461 ggggtaaact tgagttcaat cagcgcgatg gctttttgga ttgcccgcat ggtgaccggg
     2521 tctgcggcag cgggatgatg agtaaagtcg atattcttca gctgactgga cattttttca
     2581 cgaacttcaa cgggcgcgat tacatcgaga acatccagaa tttgtttgat aaccaactgg
     2641 caagctacca catcagaaac cagttcctga tcggcattca gcggctggga catcgtaaac
     2701 tcctgatagc attttgaaag ccgttatagt agcggcttca catcttcagc gatagtcaca
     2761 tccaccgtca tcaggacaca aaaaaacctg ccggagcagg ttttttgtta tcggaacata
     2821 ttgcctggcg gtacgtcttt gaacgtcttg caatagttat tgaacatact tttcaggatt
     2881 ttgcgcagtt tcatcgcggc actccgacca tttgttatac aggtgttatt gtctttgcgc
     2941 ttataatatg acaaccatca caaaaatcaa tctttatgtg atacaaatca cataaatacc
     3001 cctttaatgt tataaaaatg ataatcaaaa aacagccccc ctatttctga cacctacaga
     3061 tggcaagaaa tagcgcctgc caggcgtctt ttccggccat tgtcgcagcg ctgtaacgcg
     3121 taaaatagtg ctttctctta ctcttctggc tggaccatga gacttctgat tctgactctt
     3181 tcattaataa cgctggcggg gtgtacggtt actcgccagg cccatgtgag cgaagttgac
     3241 gcggcaacag gtattgtacg gttggtttat gaccaggctt ttttgcagca tgcccatacc
     3301 gatcgttatg tcagtcgcgg gatcgccgat cgcgcatgcc agcaggaagg ctatacccac
     3361 gcgatcccct ttggtcagcc agtaggcaac tgtagccttt ttgcgggttc cttatgtttg
     3421 aatacggaat tcactttgtc atatcagtgc catcattccg ccttccctgt ctttctctaa
     3481 atattttaat cagcgagggg atcttcgctg attaaagaaa tagcggtaat gcgttttatt
     3541 cccattcgta tttttaataa ttgaagttta tgttttacct tttgcaaata ataaaataac
     3601 aaattatagt gacgccacag caacaaatat aacctttgtg gagcactatc atgctgaaac
     3661 cagaaatgat tgaaaaactt aatgagcaga tgaacctgga actgtactct tcactgcttt
     3721 atcagcaaat gagcgcctgg tgcagctatc ataccttcga aggtgctgcc gcgttcctgc
     3781 gccgtcacgc ccaggaagag atgacgcata tgcagcgtct gtttgattac ctgactgata
     3841 ccggcaattt accgcgtatt aataccgttg aatctccgtt tgctgaatat tcctcacttg
     3901 atgaattatt ccaggaaacc tataaacacg aacaattaat cacccagaaa attaacgaac
     3961 tggctcatgc tgcaatgacc aatcaggact acccaacatt taatttcctg cagtggtatg
     4021 tttctgagca gcatgaagaa gagaaactgt tcaaatcgat tattgataaa ttaagcctgg
     4081 caggcaaaag cggcgaaggt ctgtatttta tcgacaaaga actctctacc ctcgacacac
     4141 aaaactaatg ctctccggcg gcaggtttct gccgccgttt caaatcagtg acggcaaatc
     4201 ttactctgat cggtcgaaaa accttcttct gaaggtataa atttgccttt tgctgccaga
     4261 aacgctacca gctctcccgc tgtcacccct tctgccgagc acgtgtgaaa acgtgcctgt
     4321 tcaccaaaac gcgctttaat cgcagcttcc agactggcat gcgtatattg ctcgcctgat
     4381 tcaatcatca tatttaacac ttcatgaccg tgaatagagt ccatcgttcc tcctcaaaaa
     4441 aagcctagcg tagcgattac cgcttatgaa gactttgcgc cagcgcagga ctgaatgctt
     4501 ttattgtaca tttatattta cacaatatgt aacgtcggtt tgacgaagca gccgttatgc
     4561 cttaacctgc gccgcagata tcactcataa agatcgtcag gacagaagaa agcgtgaaaa
     4621 acagaaccct gggaagtgtt tttatcgtgg cgggaaccac aattggcgca ggcatgctgg
     4681 caatgccgct ggctgcggcc ggtgttggtt ttagcgttac gttaatcttg ttgattgggc
     4741 tttgggcgtt gatgtgctac acggcgctat tactgctgga ggtgtaccag catgttccgg
     4801 cagataccgg tctgggcacg ctggcaaaac gctatctggg acgctacggt caatggctga
     4861 cgggcttcag tatgatgttc ttaatgtatg ctctgactgc agcatacatc agcggtgccg
     4921 gtgaattgtt ggcctccagc atcagcgact ggacaggtat ttcaatgtcg gcaaccgctg
     4981 gcgtgctgtt gttcactttt gttgccggtg gcgtggtttg tgttggaaca tcgctggtcg
     5041 atttatttaa ccgttttctg ttcagcgcca aaattatttt tctggtggtg atgctggtat
     5101 tgctgctacc gcatattcac aaagtgaatc ttttaaccct gccgttgcaa caggggctgg
     5161 cactgtctgc aatcccggtg atttttacgt cgtttggttt tcacggtagc gtgccgagta
     5221 ttgtcagcta tatggatggc aacattcgta agctacgctg ggtgtttata atcggtagtg
     5281 ccatccccct ggtggcatat attttctggc aggtggcgac gcttggcagc attgattcaa
     5341 caacctttat gggattgctg gctaatcatg ctggattaaa cgggctgtta caggcgttac
     5401 gcgaaatggt ggcctctccg catgttgagc tggcggtgca tttatttgct gatttagccc
     5461 tcgccacgtc atttctcggc gttgcgttag gcttatttga ttatctggct gatttgtttc
     5521 agcgttcaaa taccgttggt ggacgattgc aaactggtgc aattaccttt ctgccgccgt
     5581 tggcgtttgc actgttttat ccacgaggat ttgtgatggc gctgggttac gccggtgtgg
     5641 cgctggcggt actggcattg attatccctt cactattgac ctggcaaagc agaaagcaca
     5701 atcctcaggc gggttaccgg gtcaaaggtg gtcgtccggc gctggtggtg gtgtttctct
     5761 gtggtattgc tgtgattggc gtgcaatttt tgattgcggc agggttgtta ccagaagtgg
     5821 ggtgatcaga tagcctcaaa ttccttattg ggtgccagaa ttaacgctga cacccaatgt
     5881 gacctcttaa tgcaggcagc actgcttaaa tttcttacca ctgccgcacg gacaaggatc
     5941 gttacgcccc ggtttctctt ctgctttgat cggttgctga acagcttttt cctgcggatg
     6001 cgccatccag tatgcatgta aatcaagcgc cgccagtcga atggcatcta cgctctcttc
     6061 aaacgcttct ggcgacattt tctctacccg ctcgaagttt tcctcagtac cgtgcagcgc
     6121 aatcgcctcc agcgctggtt ttaaagaatc gggcaacgtt gaccagtcag aaagtgccac
     6181 gccccgcata tagccaaagc accactcctc aacaatcgtc agctcgctgc catcaacttc
     6241 tcgcaagccg aataacggct caaactgctc cgggaattcg ttcagacgct ctgcggtatc
     6301 ggccatatgt tgaaaagcca ggttcataaa gcgcgtcatc tctttctctg acgcccaacg
     6361 cggcacatag tcagccccac cccacacggc aaccagccac tgttctggtt caatctcttg
     6421 cggagaactc aacaccgccg tcaataaacc gtccagctcc gccacatcaa ggatggcgtg
     6481 gtcagtgttg tatttggtca gaatatcgtc cagccattcc aactcacttt cgtttaacgg
     6541 tcccgttttc atacgctttt ccttgtggat ctcaactcgc cagcacctat cttacatgcc
     6601 ggtccgtatc agagatactt tttgagtggc tttgccggtg attaaaaatt aaggagggtg
     6661 taacgacaag ttgcaggcac aaaaaaacca cccgaaggtg gtttcacgac actgcttatt
     6721 actttgattt tattcttatc tttcccatgg tacccggagc gggacttgaa cccgcacagc
     6781 gcgaacgccg agggatttta aatcccttgt gtctaccgat tccaccatcc gggctcggga
     6841 agaaagtgga ggcgcgttcc ggagtcgaac cggactagac ggatttgcaa tccgctacat
     6901 aaccgctttg ttaacgcgcc aaattcttca ggcctttcag ccagacatcc gcttgacgcc
     6961 gatgtctttt aaactggagc gggaaacgag actcgaactc gagacctcga ccttggcaag
     7021 gtcgtgctct accaactgag ctattcccgc attcatcaag caatcagtta atcacttgat
     7081 tttattatcg tctggcaatc agtgccgccg ttcgatgcgt tgcattctac ttacctggcg
     7141 cgatgagtca acgatatttt tcaccacttt tgatcgtttg ctgaaaatta cgccgaaacg
     7201 atcactgatc aagcaaatct gcacgcgcag cgctcaaata ttgcaacatt gaccacagag
     7261 tcagtaccgc agccacaaag aaaagtgcaa taccgacgta ctcaacccaa atgttcggac
     7321 gccacagcag ccaggccaac gccaccatct gggcagtggt tttcactttc ccaatccagg
     7381 agacggccac gctactgcgt ttacccaact ccgccatcca ttcgcgtagc gcagaaataa
     7441 taatttcacg ggcgatcatc gttgccgccg gtaatgtcac ccaccagctg tgataatgct
     7501 cggttaccag caccatggcg atagccacga gaactttatc tgccacaggg tcaaggaaag
     7561 caccaaaccg ggtactctgg ttccagcggc gtgccagaaa accatcgaac cagtcagtca
     7621 ccgccgcgac gcagaaaatg agcgcggcgg caaacggcga ccaggtgaca ggcagataaa
     7681 agaccaatac aaagaatggg ataaggatga cacggaacag tgtaagcaac gtagggatat
     7741 taaattgcat aatgacgggt aactatctgt tgtcagtaag attaccccta tgttgctaca
     7801 gagacatcaa tgtttcaacg accagaagat cttttctgcc agaccttgcg aaatacccgg
     7861 cacttttgca atttcctcga cgctggcgtt acgtaaacct tgcaaaccgc ccatatattt
     7921 caacaacatt tgccgacgtt ttggcccgac gccttcaatg gtttccaggg aactggtatt
     7981 tttgaccttc gcccgttttt tacggtgccc gccaatcgcg tgatcatgtg attcatcgcg
     8041 aatatgctgg ataacatgca gcgcgggtga atctggcggc aaactaaatc cctcaccttc
     8101 cggctcaaag aacagcgttt ccagtccagc cttacgatct gctcctttgg caacgccaag
     8161 tagcagcgga tgatttttat cccatgagac atccagttcg gcgaagacat ttttcgcctg
     8221 cgcaagctgg cctttgccgc cgtcgataag gatcacatcc gggatcttac tgtcgtcaat
     8281 ggctttacca taacgccgac gcagcacctg attcatcgcc gcataatcat cgcccggcgt
     8341 gatgcctgta atgttatagc gccgatactc cgcacgcagc gggccgttag catcaaacac
     8401 cacacaggaa gcgacggttt gttcgcccat ggtatggctg atgtcaaagc actccatccg
     8461 cttcacttcc ggcaatttca acacgctggc aagcgcggtc agtcgctggt gaacggtaga
     8521 ttgctgcgaa agtttgctgg ttaaggccgt cgccgcattg gtgcgcgcga gtttcagata
     8581 acgcgcccta tcgccgcgag gtttggtttg aacattaatc ttgcgtcccg ccagttctga
     8641 aagggaatcg gcgagcagcg ttttatcgct aagattaaaa tcgagcagga tctcacccgg
     8701 taaggtgcgc atctggctgc cttgtaaata gaactgacct acgaaggttt ctaccacctc
     8761 gctcagttcc gtaccgccag gcactttcgg gaaatagctg cggctgccga gcactttgcc
     8821 ctgacgaatg aacaatacgt ggacacaagc catgcccgca tcgaacgcca caccaataac
     8881 gtcaaggtcg tcgccggtat tggaaacaaa ctgtttttcg gtgacgcgtc gcaccgcctg
     8941 aatttggtcg cgaatacatg ccgcttcttc aaactccaga ttctggctgg cagtttccat
     9001 acggctaatg agttgcgtaa gcacctgatc atctttgcca gacaaaaaca ggcgcacata
     9061 ctcgacctgc tgagcgtatt cttcttcact caccagtcct tcaacgcacg gtcccagaca
     9121 gcgccctatc tggtattgca gacacggacg cgagcgattg cgataaacac tattttcgca
     9181 ctggcgaatg gggaaaatct tttgcagtag cgccagtgtt tcacgtacgg catagccatt
     9241 cgggaacggg ccgaaatatt cacctttggc atgctttgca ccacgatgca tcgccagacg
     9301 cgggtgggta tcgccactca ggaagataaa aggataagat ttatcatcgc gtagcaaaac
     9361 gttgtaacgc ggctgataga gtttgatgta gttgtgttcc agcaacagcg cttcggtttc
     9421 tgtatgcgta accgttacat caatttgctg gatctgggcg accagcgctt cggttttgcg
     9481 cgaagcgagg ttgctgcgga aatagctgga aagccgtttt ttcaggtctt tcgctttgcc
     9541 gacatagata accgtaccac cagcatcgta catgcgataa acgcctggct ggctggttac
     9601 ggtttttaaa aacgcttttg cgtcaaactg atcactcact gacttgataa tgtctccgca
     9661 ttacacagac catggcgaat tgccaggtga gtcagctcaa catcgccatg aatgtttagt
     9721 ttactgaaca tacgatagcg gtagctgttc accgttttcg gactgagatt gagctgttct
     9781 gagatctcat tgaccttctg gcccttagtg atcatcagca taatctgcaa ttcacgttca
     9841 gacaaactgg caaatgggct ttctgttttt tctggttcga tctggcttaa cgccatttgt
     9901 tgagcgatat cagaagcaat gtaacgctgc cctgaataga cagaacgaat cgcactcacg
     9961 acttcctgcg gagccgcgcc tttgctgagg tagccagcag cacctgcctg catgactttc
    10021 gctggtaaag ggttttctgt atggaccgta agcatgatga ttttaacatc agctgtggaa
    10081 cgcgcgattt tacgcgtcgc ctcaagaccg ccaatgcccg gcatactcat gtccattagc
    10141 accacgtcaa cggcatttgt ccggcaccac ttaacggcgt cttcaccgca cgatgcctct
    10201 ccgacgactt ttataccctt aatatcttcc agaatgcgtc gtatccctgc gcgcaccagt
    10261 tcgtggtcat caacaagtag aacgttgatc aaaggaatat ctccagaaat agggataacg
    10321 ctactgatag ttagtcattc gtatattagc ggtttttatt gcaactttga aacgttaaaa
    10381 atggtgggta ttcgattttt ctctcgtttt tgtcaattcg attgtccaca gttatggtca
    10441 cgcccgtcac caacggctta ccggatcgtt tcacagtaag ccctacgatg aaatgtgaca
    10501 aaaattgact ttattcagca aaaatgaaaa tcagcccgct gaatatgtaa cattaattaa
    10561 cccgaacaat acgggcaaaa aacattcatg ataaaaa
//
LOCUS       DAZSNM010000143        10489 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZSNM010000143 DAZSNM010000000
VERSION     DAZSNM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10489
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.143"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            74..523
                     /gene="alaE"
                     /locus_tag="ACHW42_002970"
     CDS             74..523
                     /gene="alaE"
                     /locus_tag="ACHW42_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR8944699.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(560..904)
                     /locus_tag="ACHW42_002971"
     CDS             complement(560..904)
                     /locus_tag="ACHW42_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR8944700.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1056..1385
                     /locus_tag="ACHW42_002972"
                     /pseudo
     CDS             1056..1385
                     /locus_tag="ACHW42_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
     gene            1633..1878
                     /gene="nrdH"
                     /locus_tag="ACHW42_002973"
     CDS             1633..1878
                     /gene="nrdH"
                     /locus_tag="ACHW42_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR8944701.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            1875..2285
                     /gene="nrdI"
                     /locus_tag="ACHW42_002974"
     CDS             1875..2285
                     /gene="nrdI"
                     /locus_tag="ACHW42_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFR8944702.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2258..4402
                     /gene="nrdE"
                     /locus_tag="ACHW42_002975"
     CDS             2258..4402
                     /gene="nrdE"
                     /locus_tag="ACHW42_002975"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR8944703.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4412..5371
                     /gene="nrdF"
                     /locus_tag="ACHW42_002976"
     CDS             4412..5371
                     /gene="nrdF"
                     /locus_tag="ACHW42_002976"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR8944704.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5726..6928
                     /gene="proV"
                     /locus_tag="ACHW42_002977"
     CDS             5726..6928
                     /gene="proV"
                     /locus_tag="ACHW42_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR8944705.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6921..7985
                     /gene="proW"
                     /locus_tag="ACHW42_002978"
     CDS             6921..7985
                     /gene="proW"
                     /locus_tag="ACHW42_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR8944706.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8043..9035
                     /gene="proX"
                     /locus_tag="ACHW42_002979"
     CDS             8043..9035
                     /gene="proX"
                     /locus_tag="ACHW42_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR8944707.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9227..10406
                     /locus_tag="ACHW42_002980"
                     /pseudo
     CDS             9227..10406
                     /locus_tag="ACHW42_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         2489 a         2674 c         2687 g         2639 t
ORIGIN      
        1 tgcagatggc aatgagatcc actgctttca tctccattaa catcccatta cgcttttatt
       61 aaggagcatt agcatgttct caccgcagtc acgcttgcgt catgcagttg cagatacgtt
      121 cgcgatggtt gtttactgtt ctgtcgtgaa catgtgtatt gaagttttcc tctccggaat
      181 gagcttcgaa cagtcttttt attccagatt ggtagcgatt ccggtgaaca tcttaattgc
      241 atggccatac ggtatgtacc gcgatctgtt tatgcgcgcg gcacgcaaag ttagcccgtc
      301 gggctggata aaaaatctgg ctgatatcct ggcttatgtg acgttccagt caccggtgta
      361 tgtggcgatc ttgttagtgg tgggcgcaga ctggcatcag attatggcgg cggtcagttc
      421 aaacatcgtt gtttcgatgt tgatgggggc ggtttatggc tacttcctcg attattgccg
      481 ccgactgttt aaagtcagcc gttaccagca ggtaaaagcc tgactcttct tttcgcgact
      541 ggcatcgcca gtcgcgtctt taactggctt cgccaaataa accattcaaa taacgttcaa
      601 gcgcaacacg cgaactaaag ccatgcggga taccgtaatg ctcgtgtcgc tcgcctgcta
      661 aatagagcgg aaattgctgg taatgatcgc aagttttaat atcggaagcg ggttcagcca
      721 gtgttgaact gcgttctttt aatgttggat gaattaccag agcggtacgt cccattcgcg
      781 cttcgcgatt aacatagaca taattttccc cacggcggta accatacgct ttctgggtta
      841 ccacatcgac agtaaaaccg actttttcaa gtacgcgcgc cacctcgtct ggtcgtaaat
      901 acatatttga tcctcgttat tttcaaccgc gggcctacct tacctgattg cgcattcaca
      961 acgctttcag aaaagtccat aaagccgcga tgaacctcag ttaagaaaat tatggtctac
     1021 actgaaaatt acatcgaatt ctaatggggg atcatatgtt taaccgaccg aaccgcaacg
     1081 acgttgatga tggcgtgcag gatatccaga atgatgtcaa tcaattagct gacagcctcg
     1141 aatctgtatt gaagtcctgg ggcagcgacg ccaaagggga agctgaagcc gcacgcagta
     1201 aagctcaggc gttgctgaaa gaaaccaggg cgcgaatgca tggtcgtact cgcgtccagc
     1261 aagccgcgcg cgatgccgtt ggctgagcgg attcttttgt tcgtgaaaga ccctggtgta
     1321 gcgtgggtac agcagctgcg gtaggtattt ttatcggcgc actgttaagc atgcgcaaat
     1381 cgtagtgcaa aaatgataat aaatacgcgt ctttgacccc gaagcctgtc ttcggggttt
     1441 ctttttgcct ggtgaatcac aaaaatcccc ctcccccgtc acgctcatat ccagggtaat
     1501 ttcgatcact atttgctata tattgtgtag ttgaatcttt tttcaactac atctagtatc
     1561 tctgtatcaa cagagagaca acccgacgcg tatcatcgcg ccgtatcttc attttaaacg
     1621 gaaatacgaa tcatgcgcat aactatttat actcgtaacg attgcgttca gtgccacgcc
     1681 accaaacggg cgatggaaaa ccggggcttt gattttgaaa tgattaatgt cgatcgcgtt
     1741 cctgaagcag cggaagcgtt gcgtgctcag ggctttcgtc agttgccggt agtgattgct
     1801 ggcgatctta gctggtctgg tttccgtccg gacatgatta accgtctgca tccagcgcca
     1861 cacgcggcca gtgcatgagc cagctcgtct acttctccag cagctccgaa aacacgcagc
     1921 gttttatcga acgtttaggt ctgcccgcgg tgcgcatccc gctcaatgag cgggaacgga
     1981 ttcaggtaga cgagccttac atcctgatcg tgccctctta cggcggcggc ggtacggctg
     2041 gcgcggtgcc acgacaggta attcgctttt taaacgacga gcacaaccgg gcgttgcttc
     2101 gcggcgttat tgcttctggt aatcgcaact ttggtgaggc gtatggccgc gccggagatg
     2161 tgattgcccg gaaatgcggc gtgccgtggc tgtaccgttt tgaactcatg ggtacgcaaa
     2221 gcgatatcga aaacgttcgt aaaggagtaa ccgaattttg gcaacgacaa ccgcagaatg
     2281 cctgacgcag gaaacgatgg attaccacgc gctgaatgcg atgcttaatc tttacgatag
     2341 cgcaggtcgc attcagttcg ataaagaccg ccaggccgtt gacgccttta ttgcgacgca
     2401 tgtgcgtccg aacagtgtga ccttcagtag ccagcagcag cgcctgaact ggctggtcaa
     2461 cgaaggttac tatgatgaaa gcgttcttaa tcgctactct cgcgactttg tcattacgct
     2521 gtttgcccac gcacacacca gcggttttcg tttccagaca ttcctcgggg catggaagtt
     2581 ttacaccagc tatacgttga agacattcga cggtaaacgt tatctggaag attttgccga
     2641 tcgagtcacg atggtggcgc tgacgctggc acaaggcgat gagacgctgg cgttgcaact
     2701 gaccgatgaa atgctgtcag gacgctttca gccagccacg ccaacattcc tcaactgcgg
     2761 taagcagcag cgcggcgaac tggtttcctg ttttttgctg cgtattgaag acaatatgga
     2821 gtcgattggt cgggcggtaa attccgcact gcagctgtcg aaacgcggcg gcggcgtagc
     2881 atttttgctg tcgaatctgc gggaagcggg cgcgccaatt aaacgtattg aaaatcaatc
     2941 ttctggcgta attccggtga tgaaaatgtt ggaagacgca ttttcctatg ccaaccaact
     3001 cggcgcgcgt cagggggcag gtgcagtcta tttacatgct catcatcccg atattctgcg
     3061 ttttctcgac acgaaacggg aaaatgccga cgaaaaaatc cgcattaaaa cactgtcgct
     3121 gggcgtggtg atcccggata tcactttcca tctggcaaaa gagaatgcgc agatggcgct
     3181 gttttcgccc tatgacgttg agcgagttta tggcaagccg tttgccgata tcgccattag
     3241 cgaacactat gacgaactgg ttgccgatga acgcatccgc aaaaaatacc tcaacgcccg
     3301 tgatttcttc cagcgactgg cagaaatcca gtttgagtcc ggctatccct acatcatgta
     3361 tgaagacaca gtaaaccgtg ctaaccctat cgccgggcgc ataaatatga gtaatctctg
     3421 ctcagaaatt ttgcaggtta acagcgcctc agagtatgac gagaatctcg actatgcccg
     3481 cacaggccat gatatttcct gcaatttagg ttcgttgaat attgcgcaca ccatggattc
     3541 ccccgatttt tcccgcacgg tagagactgc cgtgcgcggt ttaacggcgg tgtcagatat
     3601 gagtcatatc cgcagcgtgc cgtccatcga agccggaaat gccgcctcgc acgccatcgg
     3661 actggggcag atgaatttac atggttatct ggcgagggaa ggcatcgctt atggttcgcc
     3721 ggaagccctg gatttcacca atctctattt ctataccatc acctggcacg cactgcgtac
     3781 ctcgatgttg ctggcgcgcg aacgcggtga aaccttcgcc gggttcaaac agtcacgcta
     3841 tgccagtggt gaatatttca gccaatatct gcaaggtaac tggcagccga aaacggcgaa
     3901 agttggcgaa ctgtttgccc gcagctgtat tacgttacct acccgtgaga tgtgggcgca
     3961 actgcgcgac gacgtgatgc gctacggcat atacaaccag aatctccagg cggtgccgcc
     4021 aaccgggtct atctcttata tcaaccatgc tacgtcgagt attcatccga ttgtggcgaa
     4081 agtagagata cgcaaagagg gcaaaacagg acgcgtttac taccctgccc cgtttatgac
     4141 taacgaaaat ctggcgctgt atcaggacgc ttacgaaatt ggcgcagaaa agatcatcga
     4201 cacctacgcg gaagcgactc gccatgtcga tcaggggctg tcgctgacgc tgtttttccc
     4261 cgataccgcc accactcgcg atatcaacaa agcgcagatt tacgcctggc gcaaaggtat
     4321 caaaacgctc tattacatcc gcctgcgtca gatggcgctg gaaggcactg aaattgaagg
     4381 ctgcgtctcc tgtgcacttt aaggaatatc tatgaaactc tcacgtatca gcgccatcaa
     4441 ctggaacaag atatctgacg ataaagatct ggaggtgtgg aatcgcctga ccagcaattt
     4501 ctggctacca gaaaaggtgc cgctgtcgaa cgatattccc gcctggcaga cattaactgt
     4561 cgtagaacaa caactgacga tgcgcgtttt tactggcctg acgctgctcg acacgctgca
     4621 aaatgttatc ggcgcgcctt cgctgatgcc cgatgcgctc acgcctcatg aagaggcggt
     4681 attatcgaat atcagcttta tggaagcggt tcatgcccgc tcttacagtt cgattttctc
     4741 gacgctgtgc cagaccaaag atgtcgatgc cgcctacgcc tggagtgaag aaaacgcacc
     4801 gttgcagcga aaagctcaga ttattcagca acattatcgc ggtgatgatc cgctgaaaaa
     4861 gaaaatcgcc agtgtgtttc ttgaatcttt tttgttctat tccggtttct ggctgccgat
     4921 gtatttttcc agccgcggaa agctgaccaa taccgcggac ctgatccgtc tgattatccg
     4981 cgatgaagca gtccacggtt actacatagg ctataaatat cagaaaaaca tggaaaagat
     5041 atctctggga caacgtgaag agttgaagag tttcgccttc gatttgttgc tggaactcta
     5101 cgacaacgag ttgcaataca ccgatgagct gtacgccgaa accccgtggg ctgacgatgt
     5161 gaaagcgttt ctctgttaca acaccaataa ggctttgatg aatctgggct acgaaccgtt
     5221 atttcccgca gaaatggcgg aagtgaatcc ggcaatcctc gccgcgcttt cgccgaatgc
     5281 cgatgaaaat cacgatttct tttccggttc aggctcctct tatgtgatgg ggaaagcggt
     5341 tgaaacagaa gatgaagact ggaatttctg agggtgttat tttcaaaaat atcactaccc
     5401 gcagcaggga aataattccc gccaaatagc tttttatcac gcaaatattc tgtagtgatc
     5461 tacactgata ctctgttgca ttattcgcct gaaactacaa tattcaggcg ttttttcgct
     5521 atctatgaca aaaaatatca acttttctcg atttgctctc agcccttata tcacggaaaa
     5581 ttccggcgat ttgctcgcat caatattcat gccacatttg ccctcagggg ttgcctcaga
     5641 ttctcagtat gttagggtag aaaaaagtga ctattcccat tgggtaatat atcgacataa
     5701 acaaataaag aaatcattct attgcatggc cattaaatta aaaattaaaa atctttataa
     5761 aatatttggt gagcatccac agcgagcgtt caaatatatc gaacaaggac tttcaaaaga
     5821 acaaattctg gaaaaaactg ggctatcgct tggcgtaaaa gacgccagtc tggccattga
     5881 agaaggcgag atatttgtca tcatgggatt atccggctcg ggtaaatcca caatggtacg
     5941 ccttctcaat cgcctgattg aacccacccg cgggcaagtg ctgattgatg gtgtggatat
     6001 tgccaaaata tccgacgccg aactccgtga ggtgcgcaga aaaaagattg cgatggtctt
     6061 ccagtccttt gccttaatgc cgcatatgac cgtgctggac aatactgcgt tcggtatgga
     6121 attggccgga attaatgccg aagaacgccg ggaaaaagcc cttgatgcac tgcgtcaggt
     6181 cgggctggaa aattatgccc acagctaccc ggatgaactc tctggcggga tgcgtcaacg
     6241 tgtgggatta gcccgcgcgt tagcgattaa tccggatata ttattaatgg acgaagcctt
     6301 ctcggcgctc gatccattaa ttcgcaccga gatgcaggat gagctggtaa aattacaggc
     6361 gaaacatcag cgcaccattg tctttatttc ccacgatctt gatgaagcca tgcgtattgg
     6421 cgaccgaatt gccattatgc aaaatggtga agtggtacag gtcggcacac cggatgaaat
     6481 tctcaataat ccggcgaatg attatgtccg taccttcttc cgtggcgttg atattagtca
     6541 ggtgttcagt gcgaaagata ttgcccgccg gacaccgaat ggcttaattc gtaaaactcc
     6601 tggcttcggc ccacgttcgg cactgaaatt attgcaggat gaagatcgcg aatatggcta
     6661 cgttatcgaa cgcggtaata agtttgtcgg cgcagtctcc atcgattcgc ttaaaaccgc
     6721 gttaacgcag cagcaaggtc ttgatgcggc gctgattgat gcgccgttag cagtcgatgc
     6781 acaaacgcct cttagcgagt tgctctctca tgtcggacag gcaccctgtg cggtgcccgt
     6841 ggtcgacgag gaccaacagt atgtcggcat catttcgaaa ggaatgctgc tgcgcgcttt
     6901 agatcgtgag ggggtaaata atggctgatc aaaataatcc gtgggatacc acgccagcgg
     6961 cggacagtgc cgcgcaatcc gcagacgcct ggggtacacc gacgactgca ccggctgacg
     7021 gcggtggtgc tgactggctg accagtacgc ctgcgccaaa cgtcgagcat tttaatattc
     7081 tcgatccgtt ccataaaacg ctgatcccgc tcgacagttg ggtcactgaa gggatcgact
     7141 gggttgttac ccatttccgt cccgtcttcc agggcgtgcg cgttccggtt gattatatcc
     7201 tcaacggttt ccagcaattg ctgctgggta tgccagcgcc agtggcgatt atcgttttcg
     7261 ctctcatcac ctggcagatt tccggggtcg gaatgggcgt ggcgacgctg gtctcgctga
     7321 ttgccatcgg cgcaatcggt gcctggtcgc aggcaatggt gactctggcg ctggtgttaa
     7381 ctgccctgct gttctgtatc gtcatcggtt tgccgttggg gatatggctg gcgagaagtc
     7441 cgcgagcggc gaaaattatt cgtccactgc ttgatgccat gcagaccacg ccagcgtttg
     7501 tttatctggt gccaatcgtc atgctatttg gtatcggtaa cgtgccgggc gtggtggtga
     7561 cgatcatctt tgcgctgccg ccgattatcc gtctgaccat tctggggatt aaccaggttc
     7621 cggcggatct gattgaagcc tcgcgctcat tcggtgccag cccgcgccag atgctgttca
     7681 aagttcagtt accgctggcg atgccgacca ttatggcggg cgttaaccag acgctgatgc
     7741 tggccctttc tatggtggtc attgcctcga tgattgccgt cggcgggctg ggtcagatgg
     7801 tacttcgcgg tatcggtcgt ctggatatgg ggcttgccac cgttggcggc gtcgggattg
     7861 tgatcctcgc cattatcctc gatcgcctga cgcaggccgt tgggcgcgac tcacgcagtc
     7921 gcggcaaccg tcgctggtac accactggcc ctgttggtct gctgacccgc ccattcatta
     7981 agtaactctg cacttgcccg gtgacgccgg gcattatcac cctgccaaaa aaaggaataa
     8041 caatgcgaca tagcgtactt tttgcgacag cgtttgccac gcttatctct acacaaactt
     8101 ttgctgccga tctgccgggc aaaggcatta ctgttaatcc agttcagagc accatcactg
     8161 aagaaacctt ccagacgctg ctggtcagtc gtgcgctgga gaaattaggt tataccgtca
     8221 acaaacccag cgaagtggat tacaacgttg gctacacctc gcttgcttcc ggcgatgcaa
     8281 ccttcaccgc cgtgaactgg acgccactcc atgacaacat gtacgaagct gccggtggcg
     8341 ataagaaatt ttatcgtgaa ggggtatttg ttaacggcgc agcacagggt tacctgatcg
     8401 ataagaaaac cgccgaccaa tacaaaatca ccaacatcgc acaactgaaa gatccgaaga
     8461 tcgccaaact gttcgatacc aacggcgacg gtaaagcgga tttaaccggt tgtaaccctg
     8521 gctggggctg cgaaggtgcg atcaaccacc agcttgccgc gtatgaactg accaacaccg
     8581 tgacgcataa tcaggggaac tacgcagcga tgatggccga caccatcagt cgctacaaag
     8641 agggcaaacc ggtgttttat tacacctgga cgccgtactg ggtgagtaat gagctgaagc
     8701 cagggaaaga tgtggtctgg ttgcaggtgc cgttctccgc actgccgggc gataaaaatg
     8761 ccgataccaa actgccgaat ggcgcgaatt acggcttccc ggtcagcacc atgcatatcg
     8821 ttgccaacaa agcctggacc gagaaaaatc cggcggcagc caaactgttt gccattatgc
     8881 agttgccagt ggccgatatt aacgcccaga acgccattat gcatgacggc aaagcctcag
     8941 aaggcgatat tcagggccac gttgatggct ggatcaaagc ccaccagcag cagttcgatg
     9001 gctgggtgaa tgaggcgctg gcagcgcaga agtaattttt attcgggcgg ataaggcgtt
     9061 ttcgcctcat ccgccgctct gtacacaatg cctgatgcga cgctgacgcg tcctatcatg
     9121 cctacaaacc acatcgcaca tttcaacaag ctatctttca tcccatattc atcaacatcc
     9181 gctattattg atttccagct taatcatcac ctgatgaaca aaaataatga ctaaacctaa
     9241 tcatgagctt agcccggcgc tgatcgtgct gatgtctatc gccaccggtc tggcggtagc
     9301 cagtaactat tacgcccagc cattgctcga caccatcgcg cgtaactttt ccctttccgc
     9361 cagttcggca ggctttattg ttaccgccgc gcagttgggc tatgccgcag gtctactgtt
     9421 tcttgttccc ctcggtgata tgtttgaacg ccgccgcctg attgtctcga tgaccttact
     9481 ggcagcgggc ggtatgttaa tcactgccag cagtcagtca ctggcgatga tgatcctcgg
     9541 tacggcatta accggtttat tctcagtcgt ggcacaaatt ctggttccgc tggcagcgac
     9601 gctggcttca ccggataaac gcggcaaagt ggttggcacc attatgagcg gtctgctgtt
     9661 ggggatattg ctggcacgaa ccgttgccgg gttactggca aacctcggcg gctggcgcac
     9721 cgtcttttgg gttgcttcgg tgttaatggc actgatggcg ctggcattat ggcgtggtct
     9781 gccacaaatg aaatcagaaa cccacctcaa ctacccacag ttgttgggtt ccgttttcag
     9841 catgttcatc agcgataaga tcctgcgcac ccgcgcgttg ctgggctgca tgacctttgc
     9901 caacttcagc attctctgga cctcaatggc ctttttgctt gccgctccac cttttaacta
     9961 cagcgatggt gacattggtc tgtttgggct tgcgggagct gccggagcgt tgggcgctcg
    10021 tccggcgggc ggttttgccg acaagggcaa atcgcaccac aactttcggt ctgcttctgc
    10081 tgttactttc atggctggca atctggtttg gtcacacttc cgtactggcg ttgattatcg
    10141 gcattctggt gctggacctc accgtacagg gcgtgcatat cactaaccag acggtaattt
    10201 atcgaataca tcctgatgcg cgtaatcgcc tgaccgcagg ttacatgacc agctacttta
    10261 ttggcggtgc cgccggttcg ctaatttcag cctcagcctg gcaacatggc ggttgggctg
    10321 gcgtttgtct ggctggcgcg acgattgccc tggttaactt actggtctgg tggcgaggtt
    10381 ttcatcgtca ggaagccgca aattaagcaa tcagcaaccg ttttcgtggc tttacactta
    10441 taagggtgtt aagaagccca tcagtctgat aaggttaaga tattcattc
//
LOCUS       DAZSNM010000144        10464 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZSNM010000144 DAZSNM010000000
VERSION     DAZSNM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.144"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            3..203
                     /gene="cspG"
                     /locus_tag="ACHW42_002981"
     CDS             3..203
                     /gene="cspG"
                     /locus_tag="ACHW42_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR8944708.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            214..402
                     /locus_tag="ACHW42_002982"
     CDS             214..402
                     /locus_tag="ACHW42_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR8944709.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            377..607
                     /gene="ymcE"
                     /locus_tag="ACHW42_002983"
     CDS             377..607
                     /gene="ymcE"
                     /locus_tag="ACHW42_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR8944710.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            597..770
                     /locus_tag="ACHW42_002984"
     CDS             597..770
                     /locus_tag="ACHW42_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR8944711.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(819..1892)
                     /locus_tag="ACHW42_002985"
     CDS             complement(819..1892)
                     /locus_tag="ACHW42_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFR8944712.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1994..4705)
                     /gene="torS"
                     /locus_tag="ACHW42_002986"
                     /pseudo
     CDS             complement(1994..4705)
                     /gene="torS"
                     /locus_tag="ACHW42_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4788..5816
                     /gene="torT"
                     /locus_tag="ACHW42_002987"
     CDS             4788..5816
                     /gene="torT"
                     /locus_tag="ACHW42_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR8944713.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5789..6481)
                     /gene="torR"
                     /locus_tag="ACHW42_002988"
     CDS             complement(5789..6481)
                     /gene="torR"
                     /locus_tag="ACHW42_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR8944714.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6568..7740
                     /gene="torC"
                     /locus_tag="ACHW42_002989"
     CDS             6568..7740
                     /gene="torC"
                     /locus_tag="ACHW42_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR8944715.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7740..10280
                     /gene="torA"
                     /locus_tag="ACHW42_002990"
                     /pseudo
     CDS             7740..10280
                     /gene="torA"
                     /locus_tag="ACHW42_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10277..>10464
                     /gene="torD"
                     /locus_tag="ACHW42_002991"
     CDS             10277..>10464
                     /gene="torD"
                     /locus_tag="ACHW42_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR8944716.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2523 a         2685 c         2789 g         2467 t
ORIGIN      
        1 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
       61 atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat gaattccgca
      121 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
      181 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
      241 ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga gcgaaaccct
      301 ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa cgtcgcgctg
      361 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcgtgg
      421 agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa gcgataacct
      481 gttgattatt gaatctttcg ggaagatggc ttataacatt tcttacctga ccagggtacc
      541 gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg ttaacagtga
      601 atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa
      661 tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc atcgcacgag
      721 aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga ttttgtgaac
      781 atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct
      841 tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac tcccgcatac
      901 attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat taaccagcca
      961 gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt caatgttgag
     1021 gctaccggac atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga
     1081 attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc acaatcgata
     1141 caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac gcccatcagc
     1201 gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc
     1261 aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcatttta
     1321 acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg agcgggcatc
     1381 aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg cagaggaata
     1441 tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt
     1501 aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga
     1561 ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga
     1621 cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata
     1681 cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg cacccaatac
     1741 cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt cgctttacgc
     1801 caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc
     1861 cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac
     1921 caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc gcgttcaggt
     1981 ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa
     2041 ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg ctacgcattc
     2101 ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctgtcct
     2161 ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt ttaaataatg
     2221 ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg agttgcgaga
     2281 catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt aactgaccga
     2341 gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg gtacgctggc
     2401 gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct
     2461 gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa tccaccagtg
     2521 cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaatag
     2581 caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt
     2641 tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg gctgtttttg
     2701 gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg
     2761 gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca
     2821 gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg
     2881 ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc
     2941 catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg gcattgctca
     3001 ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg ctcggtacat
     3061 catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt
     3121 gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact gaaacatttt
     3181 tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc agcaacgatt
     3241 cgccagagtc ggtaatcgcc tgcaaatcat cacgctgggc gttaagtgcg gggttatctg
     3301 ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg
     3361 ccgtcagaaa cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt
     3421 gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt
     3481 cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg
     3541 tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca
     3601 gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gaatgagcga taaaccacgc
     3661 gccagaggat cataatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt
     3721 gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgtgc agctcaatgg
     3781 tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata ttattttggg
     3841 cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc aacagatcgc
     3901 tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc ggatcttcaa
     3961 tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg
     4021 gtgcattttt ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag
     4081 cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca atcagccgat
     4141 cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct
     4201 gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgctccagg cgtgcgatct
     4261 catcggcggc agctatctgc tggctgagtt gccgttgttg ctggcgtagt tgcagacgcc
     4321 gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc tgttgttcaa
     4381 tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc
     4441 tttatgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca
     4501 ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca
     4561 tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag cgcaggttgt
     4621 accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga
     4681 gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag gttgagtcgc
     4741 aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
     4801 tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac ctgttgcgct
     4861 ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc aaacgcgtat
     4921 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
     4981 gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct
     5041 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
     5101 ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag gtagcaaatc
     5161 tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc cgcgttggtg
     5221 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
     5281 aaccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt aaggagatgg
     5341 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
     5401 gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa cgccacccag
     5461 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgta
     5521 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
     5581 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
     5641 cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
     5701 cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc tcgctgtcac
     5761 cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
     5821 caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga cgaattaaca
     5881 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgcagcagac
     5941 gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt tcatactctg
     6001 cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc
     6061 aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt cgcgcgaggt
     6121 cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc agcggtttgg
     6181 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
     6241 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
     6301 cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga ttctgcataa
     6361 tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc tgagtgaagt
     6421 aggattgtaa tcgcgcctgg gtaaccggct tatcttcaac aataacaatg tgatgtggca
     6481 tcagagggtt ttactcattc tgttcatatc tgttcatatt tgctcattaa gatcgcttca
     6541 ctaaaccata attctacagg ggttattatg cggaaactct ggaacgcgct acgccgaccc
     6601 agtgctcgtt ggtcggtact ggcgctggtc gcaattggga ttgtgattgg cattgcgctg
     6661 attgtattgc cacacgttgg gatcaaagtc accagcacaa ccgaattttg tgtcagttgc
     6721 cacagtatgc aaccggtgta tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc
     6781 ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg atattccagg catggtgaag
     6841 cgcaaactgg aagcgagcaa cgacatttac cagaccttta ttgcccactc cattgataca
     6901 cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa
     6961 gaaaacaact cggcaacctg tcgctcctgc cataactacg atgcgatgga tcatgccagg
     7021 cagcatcctg aagcggcacg tcagatgaag gtggcagcga aagataatca atcctgcatc
     7081 gactgtcata aaggtattgc ccaccagtta ccggatatga gtagcggctt ccgtaagcag
     7141 ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca cgctgtactc tattgatatt
     7201 aagccaattt atgcggcgaa aggcgataaa gaagcctctg gttctctgct gcctgcttcg
     7261 gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa ttgaaattac cggctggacg
     7321 gaaagcgccg gacgtcagcg tgtactcacc caattcccag gtaaacgcat ctttgttgcc
     7381 tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg agaaaaccac cgttgccgac
     7441 accaataccg agtggagcaa gttgcaggcc accgcgtgga tgaagaaagg cgacatggtg
     7501 aacgatatca aaccgatctg ggcctatgcg gattcgttgt acaacggcac ctgtaacgag
     7561 tgccacggcg caccggaaat cgcccacttt gacgctaacg gttggatcgg cacgctcaac
     7621 ggcatgattg gctttaccag tctcgataaa cgtgaagaac gcactttgtt gaaatatctg
     7681 caaatgaatg cgtctgacac cgcaggtaag gctcacggcg ataagaagga agaaaaataa
     7741 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
     7801 taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc gcgccgtgca actgcggcgc
     7861 aagcggcgac tgaggctgtc atctcgaaag agggcattct taccgggtcg cactgggggg
     7921 ctatccgcgc gatggtgaag gatggtcgct ttgtggcggc aaaaccgttc gaactggata
     7981 aatatccgtc gaaaatgatc gccggattgc cggatcacgt acacaacgcg gcacgtattc
     8041 gttatccgat ggtacgcgtg gactggctgc gtaagcgcca tctgagcgac acctcccagc
     8101 gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag
     8161 aactggaacg cgtacagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc
     8221 aatcgacggg gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg
     8281 gtaatagcgt tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc
     8341 gcgtagtcgg ttcaatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga
     8401 acagcaaaac cattgtgctg tggggctccg atttgctgaa aaaccagcaa gcgaactggt
     8461 ggtgcccgga tcacgatgtt tatgaatatt acgagcagct aaaagcgaaa gtcgccgccg
     8521 gtgaagttga ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc
     8581 gcgagcatgt gaagcacatt gcggttaacc cgcaaactga cgtgccgctg caactggcgc
     8641 tggcacatac gctgtacagt gaaaacctgt acgacaaaaa cttcctcgct aactactgtg
     8701 tgggttttga gcagttcctg ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg
     8761 ccgcatgggc tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc
     8821 agatggcggc gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg
     8881 gtgaacagtg ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc
     8941 caggtggtgg ttttggctgg cactacaacg gcgcaggcac gccggggcgt aaaggcgtta
     9001 ttctgagtgg tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca
     9061 aaggctacag cagcactatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga
     9121 aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg
     9181 ccggaactaa cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca
     9241 agctggaaac ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata
     9301 tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact
     9361 ccaaccgtgg cattatcgcc atgaaacagg tggtgccgcc gtagttcgag gcgcgcaacg
     9421 actttgatat tttccgcgag ctgtgccgtc gctttaatcg tgaagaagcc tttaccgaag
     9481 ggctggacga aatgggctgg ctgaaacgca tctggcagga aggtgtacaa caaggcaaag
     9541 gacgcggcgt tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt
     9601 ttgaccatcc gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac
     9661 cgctgggcac gccgagtggc ctgattgaga tctactcgaa aaccatcgcc gatatgaact
     9721 acgacgattg tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc
     9781 ccggctcgca gaagtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact
     9841 cgcagctatg tgagtcggaa acgctgcgtc agcaatatac ggtagcgggt aaagagccag
     9901 tgttcattaa cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg
     9961 tctttaacgc tcgcggtcag gtgttggcag gagcagtagt ttctgaccgc tatgcacccg
    10021 gcgtggcgcg aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg
    10081 cgctgtgcaa atacggtaac cccaacgtgc tgaccatcga catcgggact tcgcagctgg
    10141 cgcaggcgac cagtgcgcac actacgctgg tggaaattga gaagtacaat ggaacagtgg
    10201 agcaggtgac ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg
    10261 cgtcgcaggt gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg
    10321 gctggcgcag ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc
    10381 gcagatggct gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa
    10441 tgatctggaa aacagtattg ccac
//
LOCUS       DAZSNM010000145        10399 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZSNM010000145 DAZSNM010000000
VERSION     DAZSNM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10399
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.145"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHW42_002992"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHW42_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFR8944717.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="ACHW42_002993"
     CDS             complement(1433..1759)
                     /locus_tag="ACHW42_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFR8944718.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="ACHW42_002994"
     CDS             1894..2235
                     /locus_tag="ACHW42_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFR8944719.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="ACHW42_002995"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="ACHW42_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="ACHW42_002996"
     CDS             complement(2833..3543)
                     /locus_tag="ACHW42_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFR8944720.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="ACHW42_002997"
     CDS             3651..3956
                     /locus_tag="ACHW42_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFR8944721.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..5568
                     /locus_tag="ACHW42_002998"
                     /pseudo
     CDS             4155..5568
                     /locus_tag="ACHW42_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(6299..7288)
                     /locus_tag="ACHW42_002999"
     CDS             complement(6299..7288)
                     /locus_tag="ACHW42_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944722.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYVLQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7373..7864)
                     /locus_tag="ACHW42_003000"
     CDS             complement(7373..7864)
                     /locus_tag="ACHW42_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944723.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(8185..8685)
                     /locus_tag="ACHW42_003001"
     CDS             complement(8185..8685)
                     /locus_tag="ACHW42_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944724.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8675..9190)
                     /locus_tag="ACHW42_003002"
     CDS             complement(8675..9190)
                     /locus_tag="ACHW42_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944725.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9621..9989)
                     /locus_tag="ACHW42_003003"
     CDS             complement(9621..9989)
                     /locus_tag="ACHW42_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944726.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
BASE COUNT         2677 a         2626 c         2569 g         2527 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc ccctatcttc tattgcacta ccgcactaga
     6001 caactagtac aaaacaaagg cccagaccat tggtccagac cgtagttagt taatcacagt
     6061 gacaagccac cacctgaagt cctgctgata ttcagtccag acttacctct gctcatggtc
     6121 ttacctccct cggtctcctg accttgggta tcgactacag cttgctgagc gtcatctgga
     6181 ggagctacag gaggagctac aggaggcggg accgaagccc caccttgagt cccaccacca
     6241 cctgtcaggc ccttggcgat ggacctgaag cctttactaa tgctcttacc cattggtctt
     6301 acgcttcggt cttggtgaac ttcaggcgtt tgactacagg caggacggca gtagctgagc
     6361 cagtgcccat cttgatggtc gtagcagctt cagcgacaac gaacacttta ccgaggattt
     6421 ccaagtcgtg ggactgagag tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt
     6481 cgtgacggaa gcagccgaag ctcagggccg tgaagcgttt agccagcatg aatacaccct
     6541 tgtcttcacc gttgtaatag gcgatgtctg tccccagcat atcgacgata gcgtcttcac
     6601 cggagaagcc ttgcttagca gccagacggg acagtgcttt cagttggctc tcatgagcga
     6661 taataccgta gtcagacacc tcagaaccga aagaagactc tgtgttctca tacagagcgt
     6721 ccatgatagc gtctgcctta gcggtcaggc taccagtcag gacaggaagc tctacggtct
     6781 tcagcagctt agaggattcc agaacgtcga ccatgcgagc gttgatggta cggaccacat
     6841 tctcacagat gttcatagcg tcctgctgaa gtgtgccctg agtgaatccg gtggatgact
     6901 tggcgtccca gagcttggtc tgctcagaga tgagaatagc ctgctcgatt ggttcgacca
     6961 cgatttcttg tccagacgga acggtcacga cttcaggtcg cttgccacca cggacaggaa
     7021 cgaccacaga gcggtcagcc agagtgtgag caatgattgg ggtcggtaag ctgttacggg
     7081 tcggcaggac ttctacaggg aacgggatgt tctcttgagc gtctttaagt acaccgttca
     7141 gcatagcctt aaccgtctta gctaactgga ggacatagac ctgcgggtca gcgttagtcg
     7201 gcagaccttc attgacctga atgttgtcgg tatcggtgat gcgtactggc tcaatgccaa
     7261 actggaaagc gtctttaatc gtagtcataa taatcaatct cctaaattag tttaaatact
     7321 gtgagtcctc aaaactgagg agtccctaac atcgggtcag ggactgtgtg ttttacaagc
     7381 tgtccatcca gttaccgcct gactcaaggt cagcgacaat agcggcacgg gagcgaccag
     7441 cggaatgggt agcactgcgg aggctctcac cataggtccg gcgttcagct ttgacctgtt
     7501 cgcggcgctg tagctctttc tgccactctg catcggtcat gttctcttca cgcatacgct
     7561 cagcctgaag tcgttgctct ttagcgatac gctcagcttc agctcgtttg ttcatctggc
     7621 ggcgaccata ggcggtcatc aagtcattac tgaagaagtt gccaatggtc acgccagctt
     7681 cagccagagc ttccagtaac tccattgcct cgcggctggt agacggcagg cctgacaggt
     7741 agcccggaat gagggtcttg atagcgagcg acagcggata gggctgaccg tccagactca
     7801 cagtgttttc ataaaggttg atgtcggtga ctgacagctt ccagttgttg ttgttgaaca
     7861 tcataggtaa ttctcctctt gagagttgag actatcggta tcgcagacgc agtagcgcct
     7921 acgggtctcc ccgcatcggt catctcaaaa ggtttaaaat taggtttggt atggtcagga
     7981 ctgcttagca aaggctcggc gtaactgatt gtctaaatca cggtccacca gcatagctat
     8041 cagctcgtct cgactgtagt aagggcgctc agggtcttcg actccaagtc tcagcagaga
     8101 cagcagatga cgtgtgggca ggccacggta acgaagggtc gctttgccga taggtgtacg
     8161 agggtcaatc atagccatga ttacttacct ccactcacag cgaccagcgg tcacagcttg
     8221 atagtctgaa tactcagcct gtgagacttc acgtccggcg atgtagaggc ctgtgccatg
     8281 acgctggatg aattccttgg tctccggctt gatgtcctca gcagacgcta cagcagactg
     8341 cacggtggcg ctggaggtca tagacacgaa ggctcgggtt actgccagtc cgtacccaag
     8401 gtccaaccac cgaccgaact ctatagcggc tactgggtca gccagccacg aggtccctaa
     8461 gtgacccatg ttaatctttt taacaaaccc gttttctggg ttggttagac ttctaccagc
     8521 cttggtcctc atgaagcgag caacctcggt actacggtta tcttctacct taccaatctg
     8581 aatgcctacc cggcgtatct catccagtcg aaacaatccg tcctgactgg tgatgtcctg
     8641 accgaagata tgaatggtgt attcctgctt attgtcagaa gtcataagag gcacctcccg
     8701 ccagagcttc ggcaaaggac ttgaagcgct gttcttggtc agctttcgcc tgaaggcgtt
     8761 cggcatcagg tgggaccagc ttatcaaagg tgccgttttc gtaatcttca gttaggacct
     8821 gaaggcggcg ggaccatttg ctctccacct gcatcatcgc gttagagatg tgcttcaagt
     8881 cggcatcaga gcgtgacgct atccaatcca tctcttctga gtcaccctca caggtgtaag
     8941 gattgttgaa atagaagatg tcctgcaggc cacgcttcag ctcttcctcg gtgtgtgtct
     9001 ggaggtatga ctggataggc tccgcgtaca tctcccggat tttctcatag tcccagtctt
     9061 ccgcctgggc ataccatcgg gataacttca gcaggtctaa gttatagcga cagcaatcga
     9121 cactggactc tgcattggtc agcaggatga ttaagaactg gcggttagtt actggtaatt
     9181 cggtattcat acaaagatgc tcctttggta aagcactttc tgtctccaca tggagtccgc
     9241 accattcaga cgcatcctaa acggacccat cagaaagtgc ttaacgaaag tgaatggtta
     9301 aatgttgacg gtctggagtg accatgttct ccatgtacgt ataaaggtgc gaccgcatgt
     9361 caggacgagc tgtaaggact ccagatggtg agcggtgata gacttaagcc aatgtagtga
     9421 gtagacgagc tactaaccga gacgactaaa gaacgctaac gagctggagg tcgatacagg
     9481 aggtctgtca gaggcggtca cgggccttga ttacttagga gagatactta atgtcggttg
     9541 accagatgct cgcatgttct gacgcatcag actcagaggc ggaggctggt ctccctatag
     9601 tgatagttta gggtccgtaa tcatgaacca gctttactgg cgtggtactg gacggattta
     9661 ctggcgctga cgacggtagc atagacgtag cgagcgtagc gagttgaaac cataggttca
     9721 cccaaggcaa cacgcttacg gctcagggac tcctgcacag cgcttacagt taatgtgtcg
     9781 tgcgttagta gttgggtcag aatcaagtat gggctgacag gcttggtatt gcttgccatt
     9841 gattcgttca ggcgaccagc accagtaagg acatcatcca gccaaccact caggtcgccc
     9901 tgaccatcga atgacagcag gtccccgcag tgctgctgca agtaggcttt ggtgaacgtg
     9961 ttgtcgtgag ttgcgattgg tcgtgccata gagtatctcc tgtagttgaa ttagttggtt
    10021 cccctatagt gataatcaag tccatcactg gtgatgcata cagtagcgcg acagaatgtg
    10081 actcacaggt tgaaccgaat gtcaaggact aaagacgcct caggaattaa ggaagtatat
    10141 ataaagagag accagaggcg actcacagtt aaacaggaat gtcaagagga gaccatagga
    10201 atttggaggg actggagttt aggacgcagt ttaaataaga ccacagacag acttagagtc
    10261 ggacagccga aggcggacca gtgggcctat agtcaggagg gattatgatt attatcaccc
    10321 tccctaactt cagtcagact tcagtgaccc aaggtctcaa ccatcaagcc gtatatggtc
    10381 tccaccttgg gtctcccta
//
LOCUS       DAZSNM010000146        10380 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZSNM010000146 DAZSNM010000000
VERSION     DAZSNM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10380
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.146"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            2..1369
                     /gene="dcuC"
                     /locus_tag="ACHW42_003004"
     CDS             2..1369
                     /gene="dcuC"
                     /locus_tag="ACHW42_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR8944727.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACHW42_003005"
     CDS             complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACHW42_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR8944728.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACHW42_003006"
     CDS             complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACHW42_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR8944729.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACHW42_003007"
     CDS             complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACHW42_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR8944730.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACHW42_003008"
     CDS             complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACHW42_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR8944731.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACHW42_003009"
     CDS             complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACHW42_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR8944732.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5324..5722
                     /gene="zapG"
                     /locus_tag="ACHW42_003010"
     CDS             5324..5722
                     /gene="zapG"
                     /locus_tag="ACHW42_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR8944733.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            5876..7223
                     /gene="degQ"
                     /locus_tag="ACHW42_003011"
                     /pseudo
     CDS             5876..7223
                     /gene="degQ"
                     /locus_tag="ACHW42_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            7313..8380
                     /gene="degS"
                     /locus_tag="ACHW42_003012"
     CDS             7313..8380
                     /gene="degS"
                     /locus_tag="ACHW42_003012"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR8944734.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACHW42_003013"
     CDS             complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACHW42_003013"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR8944735.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            9815..10285
                     /gene="argR"
                     /locus_tag="ACHW42_003014"
     CDS             9815..10285
                     /gene="argR"
                     /locus_tag="ACHW42_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR8944736.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2531 a         2644 c         2606 g         2599 t
ORIGIN      
        1 aatgttcggc ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa
       61 aaactacaaa cctcaggtgg tgctggctgc cgcaggtgtc ttcctgatga tgtgcggagt
      121 ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga
      181 catttataat gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat
      241 tatggcggtg ggcggttatg cccgctacat ggagcgcata ggagccagtt gcgcgatggt
      301 gagcttgcta agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac
      361 ttacgtcatc ggccaaatca tggcgcagtt tattaccagc gcctccggtc tgggtatgtt
      421 gctgatggtc accttatttc cgacgctggt gagtctggga gtaagtcgcc tctctgcggt
      481 ggcggttatc gcaaccacga tgtccattga gtgggggatt ctggaaacga actccatttt
      541 tgcagcccag gtcgcgggaa tgaaaattgc cacatacttc ttccactacc agcttccggt
      601 cgcctcttgc gtcattatct cggtggcgat ctcccacttt ttcgtgcaac gcgcttttga
      661 caaaaaagat aaaaatatca atcacgaaca ggcagagcaa aaaactctcg ataatgtccc
      721 gccgctctat tacgccattt tacctgtgat gccgttaatc ctgatgctcg gctcgctgtt
      781 cctcgcccac gtcgggctga tgcagtcaga actgcatctg gtggtggtga tgttactaag
      841 tttgacggtg acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga
      901 cgatgtgcag gcgttttttg acggcatggg tacgcagttt gtcaacgtgg taacgctggt
      961 ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt ggcactgttg atgcggttat
     1021 caggggtgcg gagcattctg gtctgggcgg tattggcgtg atgattatta tggcgctagt
     1081 cattgccatt tgtgccattg tgatgggctc tggcaatgcg ccgtttatgt catttgccag
     1141 tcttattccg aatatcgcag ccggactaca tgtaccagcg gttgtaatga ttatgccgat
     1201 gcattttgcc acgacgctag cgcgcgcggt ttcgccgatt actgcggtgg tggtcgttac
     1261 gtcaggaatt gcaggcgttt cgccttttgc ggtggtgaag cggacagcga tccccatggc
     1321 agtcggtttc gtggtgaata tgattgccac aatcacgcta ttttattaag tcattaaaaa
     1381 gacaaaacag gccgcctggg cctgttttgt attacttcac aacgcgtaat gccggtcgac
     1441 caccgcgtgg tggctgcgga ggttcatcgt caggatgagt gtcatcatcg tgatctggct
     1501 tgtcgccatc aataaccgac ataacggttt cgttgtctgc cgatgcctct tcatcattca
     1561 tgatgctggt atcttcatcg taggcagctt caggctcaaa catcgtgcct gcgccatttt
     1621 cacgggcgta gatagccagc acggcagcca gcggcacaga aacctgacgc ggaatgccac
     1681 caaagcgcgc gttaaagcgc acctcatcat tcgccagttc cagattgccg acagcacgcg
     1741 gcgcaatgtt gagtacgatt tgcccgtcac gcgcatattc cataggaacc tgcacgccag
     1801 ggagcgtcac atccaccacc aggtgcggcg tgagctggtt atccagcaac cactcataga
     1861 atgcacgcag cagataggga cgacgtggtg ttagctgtga caaatccata cagattaact
     1921 ccggcccaga cgcatttcac gttctgcttc agttaaagaa gcaaggaaag agtcacgctc
     1981 aaagacgcgg gtcatatagc ctttcagctc tttcgcaccc gggccgctga actcgatgcc
     2041 cagttgcggc agacgccaca gcagcggagc aaggtagcaa tcgaccaggc tgaactcatc
     2101 gctcaggaag tacggcttct gaccgaagac cggcgcaatc gccagcagtt cttcgcgcag
     2161 ttgcttacgt gcggcatctg cttcagaagc tgaaccgttg atgatagtgt tcatcagcgt
     2221 gtaccagtct ttttcgatgc gatgcatgta cagacggctt tcaccgcgag ctaccgggta
     2281 aacaggcatc agtggcggat gcgggaaacg ctcatccaga tattccataa tgatgcgaga
     2341 ttcccacagg gtcagctcac gatccaccag ggtcggaacg ctctgattcg ggttgaggtc
     2401 aatcagatcc tgaggcggat tgtccttttc cacgtgttcg atctcgaaac ttacaccttt
     2461 ctcagccagc acaatgcgga cctgatggct atagatgtca gtaggaccgg aaaacagcgt
     2521 cattaccgaa cgtttgttgg cagcgacagc catgaaaacc tccaggtata gtcagaattt
     2581 ttactgctac cagccaccag gtggccagtc agaagttgtg ttacccaata aggaacgact
     2641 ctctttgttc gaaaatcaaa caaaaaatga gcaatacccg acatttgggc agaaaattgg
     2701 atgatagttt accagatttt gcgacctttg tggtgagtgg atgccggaaa tggggaaaaa
     2761 gagatgcgct ttagtctgaa atagttgact tagaccctta ttggcgatgt ggtttttgtt
     2821 ttacctgtct ttcagacggc agcaaaaagc aacttcccat tttttacgct gattcagaat
     2881 ttagccataa aaaaacccgc cgaagcgggt tttttcgcaa attgttttct gccgtagcag
     2941 aagccaatta acgtttggag aactgcggac gacgacgtgc tttacgcaga ccgactttct
     3001 tacgttcaac ctgacgagcg tcacgagtaa cgaagccagc tttacgcagt tcagaacgca
     3061 gggactcgtc gtattccatc agagcgcggg tgataccgtg acggatcgca ccagcctgac
     3121 cagagatacc accaccttta acggtgatgt acaggtccag tttctcaacc atgtcgacca
     3181 gttccagcgg ctgacgaact accatgcggg cagtttcacg accgaagtac tgttccagag
     3241 aacgttggtt gattacgatt ttaccgttgc ccggtttgat gaaaacgcga gctgcggaac
     3301 ttttgcggcg accagtgccg tagtattgat tttcagccat tgcctataat cccgattaga
     3361 tgtcaagaac ttgcggttgc tgtgccgcgt ggttgtgctc gttacccgcg taaactttca
     3421 gtttacggaa catagcacga cccagcgggc cttttggcaa catgccttta accgcgattt
     3481 caatcacacg ctcaggacgg cgagcaatca tctcttcaaa ggtcgcttgt ttgataccac
     3541 cgatgtggcc ggtgtggtga tagtacactt tgtcagtacg cttgttgccg gttacagcaa
     3601 ctttgtcagc gttcagaacg atgatgtaat caccggtatc tacgtgcgga gtatattccg
     3661 ctttgtgctt accgcgcagg cgacgagcca gttcagtagc cagacggccc agagttttac
     3721 cggtcgcgtc aacaacatac cagtcgcgtt ttacggtttc tggtttagct gtaaaagttt
     3781 tcattaaaag cttacccaat aaatagttac acgttggtga acacccaaac gtcttcaatt
     3841 gttgaggttc acacgacaaa gtccggcaaa cctacccctt cgaatagcct atgccagcac
     3901 acaaaaagtt ttgggaaaaa aactttcttg taacgtgggg tcgcaggatt atagagaagt
     3961 cggggtcaaa gatcgacccc tttttgtgat ttgtggcagg ttttaacccg ccaaatgctc
     4021 gcgcttcaga tactcttcgc tttgcatctc ttgcagacgt gacaggcaac gctggaactc
     4081 aaacttcagc cgctcaccct gataaatttc atacagcggc acttctgcac tcaccactaa
     4141 tttgacatgg cgctcgtaaa actcatccac cagcgcaata aagcgccgcg cttcgctctc
     4201 catcaaccgc gtcatgactg gcacatcaaa caacattact gtgtgaaaca ggcgtgagag
     4261 cgcaatatag tcatgctgac tgcgggcgtc gacgcacagc gtagtaaaag aaaccgccag
     4321 cgtctggttc tcgacgccca ttgtcgccaa tggccgatgg ttgatttcta acgtcggtga
     4381 attttctcgt ttcacccccg ccagcgccaa ccatagttta tccatctgcg cccgggtttc
     4441 atcgttgagt ggcgaaagcc acagatgcgc ctgagtgagt gtacgcagtc gataatcaac
     4501 accagcgtcc acgttcatta catcacaatg ctgtttaatg gcatcgattg caggcagaaa
     4561 acgcgcacgt tgcaggccat ttcgataaag ttcgtccggc ggaatatttg acgtcgctac
     4621 cagggtaata ccgcgggtga acagggcttt catcagaccg ccaagtagca tggcgtcggt
     4681 aatatcagaa acaaaaaatt cgtcaaaaca gagcacgtca gtttcggctt taaagcgatc
     4741 ggcaataatt tccagcggat cggtctgccc ctgtaaggca gttagctctt cgtgcacccg
     4801 cagcataaaa cggtgaaagt gcaggcgctg tttccgctct cccggcaggc tttgatagaa
     4861 aaggtccatc agccaggttt tcccgcgtcc tacaccgccc cacatatata agccacgcac
     4921 tggcgtatgc tttgtgtctt cgcgtttacc ccacagctta ccgacccgcg ccattagccc
     4981 actcgtcctg ggggctggtg gcctgctatt gatgagttct tgataaataa tttccaggcg
     5041 gctgacggcc tctttttgaa cgtcgtcggg ttgatggctg ccttcattga gcgccttcag
     5101 gtattgcgat gttggggtaa cgctttgcat gatcttattg ttattccttg aataatcggt
     5161 gcgccgttgt tcacggttga cgaaaaaaag gccgttctac actacgcgat atgcagtcgg
     5221 gattccactt ctgtggaatt aacggttata gtggcataat cagccgcagg catggagcct
     5281 gaagccaaca ccctacggaa acaaaagaca acgggagatg ttcatgacct gggaatatgc
     5341 gctaattggg ttagtcgtcg gcatcattat tggtgctgtg gccatgcgtt ttggtaatcg
     5401 taaactacgc cagcaacagg cgttgcagta cgaactggaa aagaataaag ctgaactgga
     5461 cgagtatcgc gaagagctgg ttagccactt tgcccgcagc gcggaattac tggataccat
     5521 ggcgcacgac tatcgccagc tgtatcagca catggcaaaa agctccagca gcctgctgcc
     5581 ggaactgtct gctgaagcaa acccgttccg taatcgtctg gcagagtctg aagccagcaa
     5641 cgatcaggca ccggtgcagg tgcctcgcga ctattctgaa ggcgcatccg gcctgctgcg
     5701 tactggcgcg aagcgcgact aatttatttt tcgggcgcag ccattgcgcc ctcctcttct
     5761 ctccctcccc gactatcatt taatctggtg tctcattgtt agccgtctga aaattcaata
     5821 acatcaaact gttttgaatc tcttttctta tcattcaggt acgagagcag gaataatgaa
     5881 aaaacaaacc cagctgttga gtgcattagc gttaagtgcc gggttaactc tctcggcgtc
     5941 atttcaggcc gtcgcgtcga ttccaggcca ggttgccgat caggcccctc tccccagtct
     6001 ggctccaatg ctggaaaaag tgcttccggc agtggtgagc gtacgggtgg aaggaacggc
     6061 cagtcaggga cagaaaatcc cggaagaatt caaaaagttt tttggtgatg atttaccgga
     6121 tcaacctgca caacccttcg aaggtttagg ttccggtgtc atcatcaacg ccagtaaagg
     6181 ctatgtgctg accaacaacc atgtgattaa tcaggcacag aaaatcagta ttcagctcaa
     6241 tgatgggcgc gagtttgatg caaaactgat tggtagcgat gaccagagcg atatcgccct
     6301 gttacaaatt caaaacccga gcaaattaac gcaaatcgct attgccgact ccgataaatt
     6361 gcgcgtcggt gattttgccg tagcggtcgg taacccattt ggccttgggc aaaccgccac
     6421 ctctggcatt gtttccgcat taggccgcag cgggttgaat cttgaaggtc tggaaaactt
     6481 tatccagaca gatgcttcca ttaaccgcgg taactccggc ggtgcactat taaaccttaa
     6541 cggtgagtta attggcatca acactgcaat ccttgcgcct ggcggcggga gcgtcgggat
     6601 tggatttgcc atccccagta atatggcgcg aacactggcg cagcagctta tcgactttgg
     6661 tgaaatcaaa cgcggtttgt taggcatcaa aggcaccgag atgagtgccg atatcgccaa
     6721 agccttcaac cttgacgtgc agcgtggcgc gtttgtcagc gaagtgttgc caagttctgg
     6781 ctcagcgaaa gcgggcgtca aagcgggcga tattattacc agcctcaacg gcaaaccgct
     6841 gaatagcttt gctgagttgc gctctcgtat cgcgactaca gagccgggca cgaaagtgaa
     6901 gcttggcctg ctgcgtaacg gcaaaccact ggaagtagaa gtgacgcttg ataccagcac
     6961 ctcttcgtcg gccagcgctg gaaggtgcaa cgttgagcga tggtcagcta aaagatggcg
     7021 gcaaaggtat taagatagat gaagttgtca aaggaagccc agctgctcag gctggcttgc
     7081 aaaaagacga tgtgatcatt ggcgtcaacc gcgatcgggt gaactcgatt gctgaaatgc
     7141 gtaaagtgct ggcggcaaaa ccggccatca tcgccctgca aattgtacgc ggcaatgaaa
     7201 gcatctatct gctgatgcgt taatgtcgta aaccgggcat caggctgacg tgtgatgtcc
     7261 ggttaactcg tggtatgctg ctgccgttcc cttttttaat gacgcctcca tcatgtttgt
     7321 gaagctctta cgttccgttg cgattggatt aattgtcggc gctattctgc tggttgccat
     7381 gccttcgctg cgcagcctta acccgctttc cactccgcaa tttgacagta ccgatgagac
     7441 gcctgccagc tataatctgg cggttcgccg cgccgcgcca gcggtggtta acgtttacaa
     7501 ccgtggtttg aacaccaact ctcacaacca gcttgagatc cgcaccctgg gatccggtgt
     7561 aatcatggat caacgcggtt atatcatcac caataaacac gtcatcaacg acgccgatca
     7621 gatcatcgtc gccttacagg atggacgtgt atttgaagca ttgctggtgg gatctgactc
     7681 tctaaccgat ctggcggtac ttaaaattaa tgccactggc ggtttaccta ccattccaat
     7741 taatgcacgt cgcgtaccgc acattggcga cgtagtactg gcgatcggta acccgtacaa
     7801 cctcgggcag accattaccc aagggattat tagtgccacg ggtcgaatcg gtctgaaccc
     7861 gaccgggcgg caaaacttcc tacaaaccga tgcttccatt aaccacggta actctggcgg
     7921 cgcgctggtg aactcgctgg gagaactgat gggcattaac acgctgtcgt ttgataagag
     7981 taacgatggc gaaacgccgg aaggtatcgg ctttgcgatt cctttccagt tagcaaccaa
     8041 aattatggat aagctgatcc gcgatggtcg cgtgatccgc ggctacattg gtatcggcgg
     8101 acgcgagatc gcaccactgc acgcgcagag cggtggtata gatcaactgc aagggatcgt
     8161 ggttaatgaa gtgtcacctg acggcccagc ggcgaatgcg ggtattcagg tcaacgatct
     8221 gattatttcg gtggataaca aaccggccat ctctgctctg gagacgatgg atcaggtggc
     8281 ggaaattcgc cctggttcgg tgatcccggt tgtagtgatg cgtgatgata agcagttaac
     8341 gctgcaggtc accattcagg aatatccggc aaccaattaa gtcgagtgct caaaacaaaa
     8401 accggagtct gtgctccggt tttttattat tcgctaatca attacttatt aacgaactct
     8461 ttgcccaggg cgatatcttt cttgagcgta tccagcatac cttccagcgc gttctgttca
     8521 aatgcgctca gggtaccgat agatttacgc tcttccacgc cgtttttacc cagcagcagc
     8581 ggttgagaga agaaacgggc gtactgaccg tcgccttcaa cgtaggcaca ttcgacaacg
     8641 ccttgttcgc cctgcagtgc acgaaccaga gacagaccaa aacgtgcagc tgcctggccc
     8701 atagacaggg ttgcagaccc gccaccggct ttcgcttcaa ccacctcagt acccgcgttc
     8761 tggatacgtt tggtcagatc agccacttcc tgctcggtaa aactaacgcc aggaacctgt
     8821 gacagcagcg gcagaatggt aacaccagag tgaccgccaa taaccggcac ttcaacttcg
     8881 cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg aacgaatgat atccagcgtg
     8941 gtaacgccga acagtttgtt tttgtcataa acaccggctt ttttcagcac ttcagcagca
     9001 attgcaactg tggtgttaac cgggttagtg ataataccaa tgcacgcttt cgggcagttt
     9061 ttcgcaactt gctgtaccag gtttttcacg atgccggcgt taacgttaaa caggtcggaa
     9121 cgatccatac ccggtttacg cgctacgcct gcagagataa gaacgacatc tgcgccttcc
     9181 agcgccggag tcgcatcttc accagaaaaa cctttgattt tcacagcagt agggatatgg
     9241 ctcagatcga cggccacacc gggagtcact ggagcgatat catacagaga gagttctgaa
     9301 cctgaaggca gttgggtttt taacagtagt gcaagcgcct ggccaatacc gccagcagcg
     9361 ccgaggactg cgactttcat cctaaactcc ttattatatt gataaactaa gatatgttgc
     9421 tccgctgccg cgaccttaat ccacaaaatt gccgtttaca atgaccacat ctcaagaatg
     9481 tgtagtcacg caagtttagc gtttatgcat ttaattgccg taatcaggaa cctaacttac
     9541 gtaattaaca gtcgcttatg cattagcgca acattccaac aggtggtgac aatataccct
     9601 accgttcagc caaaacaaca tcaatttgat aacaattaat ttacttttaa acagaatttg
     9661 catgccgtga cgcaggcatg tttctcaata acgaaatttg ataaaatccc gctctttcat
     9721 aacattattt cagccttctt cagggctgac tgtttgcata aaaattcatc tgtatgcaca
     9781 ataatgttgt atcaaccacc atatcgggtg acttatgcga agctcggcta agcaagaaga
     9841 actagttaaa gcatttaaag cattacttaa agaagagaaa tttagctccc agggcgaaat
     9901 cgtcgccgcg ttgcaggagc aaggctttga caatattaat cagtctaaag tctcgcggat
     9961 gttgaccaag tttggtgctg tacgtacacg caatgccaaa atggaaatgg tttactgcct
    10021 gccagctgaa ctgggtgtac caaccacctc cagtccattg aagaatctgg tgctggatat
    10081 cgactacaac gatgcagttg tcgtgattca taccagccct ggcgcggcgc agttaattgc
    10141 tcgcctgctg gactcactgg gcaaagcaga aggtattctg ggcaccatcg ctggcgatga
    10201 caccatcttt accacccctg ctaacggttt caccgtcaaa gacctgtacg aagcgatttt
    10261 agagctgttc gaccaggagc tttaatctct gccccgtcgc ttctggcggc ggggaaaatg
    10321 ttgcttatcc ctctcattcc ccttctttct cctgcgatac atttaacgaa tagtgcgttt
//
LOCUS       DAZSNM010000147        10252 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZSNM010000147 DAZSNM010000000
VERSION     DAZSNM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10252
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.147"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            269..358
                     /locus_tag="ACHW42_003015"
                     /pseudo
     CDS             269..358
                     /locus_tag="ACHW42_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            660..1655
                     /gene="pgl"
                     /locus_tag="ACHW42_003016"
     CDS             660..1655
                     /gene="pgl"
                     /locus_tag="ACHW42_003016"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR8944737.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1696..2649)
                     /locus_tag="ACHW42_003017"
                     /pseudo
     CDS             complement(1696..2649)
                     /locus_tag="ACHW42_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2834..3886
                     /locus_tag="ACHW42_003018"
     CDS             2834..3886
                     /locus_tag="ACHW42_003018"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFR8944738.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3962..5395
                     /locus_tag="ACHW42_003019"
     CDS             3962..5395
                     /locus_tag="ACHW42_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFR8944739.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5578..7758
                     /locus_tag="ACHW42_003020"
     CDS             5578..7758
                     /locus_tag="ACHW42_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR8944740.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7899..9182)
                     /locus_tag="ACHW42_003021"
     CDS             complement(7899..9182)
                     /locus_tag="ACHW42_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR8944741.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9317..>10252)
                     /locus_tag="ACHW42_003022"
     CDS             complement(9317..>10252)
                     /locus_tag="ACHW42_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8944742.1"
                     /translation="TEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILAS
                     RGIKQKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTL
                     SDAFREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMEL
                     AVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCK
                     EILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQ
                     ISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2505 a         2385 c         2571 g         2791 t
ORIGIN      
        1 actacaacat ctccaactat tttagccagc ctgactactt gacttggtaa tatttgttgc
       61 taataatcat gtataaaata agcttatata catagtaact tcagcaaagg atttagtaat
      121 gatcaaaatc atatctcaat gtactgtttt tttctttaaa aaaacagtac attctatttt
      181 ataatgactg tgtattccca tggaaggaga tcacccaatt ctcccccccg acttttgacg
      241 cagtgtcttg ttaacagggg gattgaccat gcttattggc tatgtacgcg tatcaacaaa
      301 tgaccagaat accgatctac aacgtaatgc gtttatttgt gccggaagtg agttgattgt
      361 tgcgcgaatg ggaagttggc cagaagttgt tatggcatag tgtttacggt cgttgataca
      421 ggtaaacaaa agcggggaga gtcatactcc ctgaatagtc gcgaaaatat ctgtggcatt
      481 gtcaaattcg tgtcggtgac taatatttta gtaatatgcc attcgaaatg gagtgctaac
      541 ttattgattt aattgatgtg cgatattgta ttttatcgtg ttttgttgtc tgttttttat
      601 ccagtaagtg ttgttatatg tatgaaagat aagggattta tcatttttga ggtgtgttca
      661 tgaagcaaac agtttatatc gccagccctg agagccagca aattcacgtc tggaacctga
      721 atcatgaagg cgcactgacg ctgacacagg ttgtcgatgt gccggggcag gtgcagccga
      781 tggtggtcag cccggacaaa cgttatctct atgttggtgt tcgccctgag tttcgcgtcc
      841 tggcgtatcg tattgccccg gacgatggcg cactgacctt tgccgcagag tctgtgctgc
      901 cgggtagtcc gacgcatatt tccaccgatc accaggggca gtttgtcttt gtaggttctt
      961 acaatgcggg taacgtgagc gtaacgcgtc tggaagatgg cctgccagtg ggcgtcgtcg
     1021 atgtagtcga ggggctggac ggttgccatt ccgccaatat ctcaccggac aaccgtacgc
     1081 tgtgggttcc ggcattaaag caggatcgca tttgcctgtt tacggtcagc gatgatggtc
     1141 atctggtggc gcaggaccct gcggaagtga ctaccgttga aggggccggc ccgcgtcata
     1201 tggtattcca tccaaacgaa caatatgcgt attgcgtcaa tgagttaaac agctcagtgg
     1261 atgtctggga actgaaagat ccgcacggca atatcgaatg tgtccagacg ctggatatga
     1321 tgccggaaaa cttctccgac acccgttggg cggctgatat tcatatcacc ccggatggtc
     1381 gccatttata cgcctgcgac cgtaccgcca gtctgattac cgttttcagc gtttcggaag
     1441 atggcagcgt gttgagtaaa gaaggcttcc agccaacgga aacccagccg cgcggcttca
     1501 atgttgatta cagcggcaag tatctgattg ccgctgggca aaaatctcac cacatctcgg
     1561 tatacgaaat tgttggcgag caggggctac tgcatgaaaa aggccgctat gcggtcgggc
     1621 agggaccaat gtgggtggtg gttaacgcac actaaccgct gatttaaccg gcgcagtctc
     1681 tcctgcgccg gtgtattaac ttatctcctg taacgcatgt ctctggcgtt cgacgatatt
     1741 ggtccacaaa ttgtctttat cgtcagtcca caaattaatc agcaaggaaa aaaagcgttc
     1801 agctgccgga gaaagcacgg catctttgcg gcgaataatc cccaatgtcc gacgtataac
     1861 gggttccacc agtgggatac caataatcga agaatagggc gcatggggca ttgccagacc
     1921 aggaagcgcc gaaatcccca gtcctgcctc cactagtcct aatgacgtcg aaagatgacg
     1981 cacttcgtaa aaccagtcca gcttccaggg cttgtcggct agctgttgtt ctatcaacaa
     2041 tcggttgccg ctggaggaac gtacgccaat cattttgtaa cccactaact cctgccattc
     2101 tacaagctac tttttggcca atggatggtc acgccgacag gccagcacga atggctcgtt
     2161 gaccagtggg gtaaaatcaa tggatgaatt tgtgacgttg ttcatgttta tgccaaagtc
     2221 tgattcgtta cacagcaccg attccatgca attattcgtg ccttgttcca gaatccgcac
     2281 tttaatattg ggatacagct cattaaattt accgattgcc agcggtaaaa aataaaatac
     2341 tgcggtcgga atacacgcta atgtcaccat accacgatga taagcattca tatcacgaat
     2401 attaaaaagc gtttcatcga atttctttat taattcccgc gcctccggga gcaacctttt
     2461 tcctgctttt gttaacgtaa ccttgcgagt tgtacgctca aatagttgaa tatgtaaatc
     2521 ttcctccatc ttttttattc ttcgcgttaa tgcaggttgc gtaatattga gtaatttagc
     2581 ggcattgtta aatgaactgg actctgccag tatgacaaat gccttcatac ttgataattc
     2641 atgcttcatt attactccgg aaaatggaag cgacgatttt gggggggctg gtcgttaaaa
     2701 attttaactg catttagcca acttaaatta atgaaaaaat gttattaatc gttgagctaa
     2761 agtcattaga gatgctttgt ccttaatgta accatatcgc aataagttat gtttttaaat
     2821 tgagggcatt attatgaaaa aaataccctg cgtgatgatg cgaggtggaa cctcgagggg
     2881 cgcgttcctg ttagcggaac atttacccga agatcaaacg cagcgcgata aaatattgat
     2941 ggcaattatg ggttccggta acgatctgga aattgacggt attggcggcg gtaatccact
     3001 gaccagtaaa gtcgccatta ttagccgttc cagcgatccg cgtgctgatg tcgattatct
     3061 gtttgctcaa gttatcgtcc atgagcaacg tgtcgatacc acgcctaact gcggcaatat
     3121 gctgtctggc gtaggggcat tcgccattga aaatggtttg attgcagcga cttcgccagt
     3181 tacccgtgta cgtatccgca acgtcaatac gggtacgttc atcgaagctg atgtgcaaac
     3241 gccaaatggt gttgtcgagt acgagggtag cgccagaatt gacggcgtac cgggtactgc
     3301 cgcaccggtt gcgctcactt tcctgaatgc cgctggaacg aaaactggaa aagttttccc
     3361 gactgataat cagattgatt attttgacga tgtcccggtg acctgtatcg atatggcgat
     3421 gccagtcgtc attattccgg ctgaatatct gggaaaaaca ggttatgaat taccggcgga
     3481 actggatgcc gacaaagcat tattagcccg cattgaatct atccggctac aagcgggtaa
     3541 agcaatgggc ttaggtgatg tcagtaatat ggttatccct aaacctgtgc ttatttctcc
     3601 agcgcagaaa ggcggggcaa ttaatgtgcg ttattttatg ccgcattctt gccatcgcgc
     3661 gctggcgata accggtgcta ttgctatttc cagtagttgt gcattggaag gcaccgtcac
     3721 ccgacaaatc gtcccttctg taggatatgg caatatcaat attgaacacc ccagtggtgc
     3781 gctcgacgtt catttaagta atgaaggtca ggatgccacg acgttacgcg catctgttat
     3841 tcggacaacc agaaaaatat tttccggtga agtttatctt ccctgaaaaa attcgttgtc
     3901 aggataagga caatcaataa aggacttctg tatgagtcat acagaaagaa caggatttta
     3961 aatgaataag aaatcgttat ggaagctaat tctgatatta gcgatcccat gtattattgg
     4021 ttttatgcca gctccggcag gattaagcga actggcgtgg gtgctttttg gtatttacct
     4081 ggcggccatt gtgggtctgg ttatcaagcc tttcccggaa cctgtcgtac tgttaattgc
     4141 cgttgctgcc tcaatggtgg tggtcggtaa cttatccgac ggtgcgttta agaccaccgc
     4201 cgtattaagc ggttactctt caggtactac ctggctggtg ttctcggcgt ttaccttaag
     4261 cgccgcattt gtgaccaccg gtttaggtaa acttattgcc tatctgctga ttggtaaaat
     4321 cggtaacacc atgctgggtc tgggttacgt tacggtattc ctcgatctgg tactggctcc
     4381 ggcaacaccg tctaacaccg cgcgtgcggg cggcattgtg ttaccgatca tcaacagcgt
     4441 ggcggtggct ttggggtccg aaccggaaaa aagtccgcgt cgtgtcggac attacctgat
     4501 gatgtccatt tacatggtca ccaaaaccac cagctatatg ttctttaccg caatggcggg
     4561 gaacattctg gcgctgaaaa tgatcaacga cattctgcac ctgcaaatta gctggggtgg
     4621 atgggcgctg gccgccggat tgccgggcat cattatgctg ctggtcaccc cgctggtaat
     4681 ttacaccatg tatccaccag aaattaaaaa ggtggataac aaaaccatcg ctaaagcggg
     4741 ccttgccgaa ctgggaccga tgaaaatccg cgaaaaaatg ctgctcggtg tctttgtgct
     4801 ggcgctactg ggctggattt tcagtaagtc tctgggggtt gatgaatcca ctgtggcaat
     4861 cgttgttatg gcgaccatgc tgctgctggg tatcgttacc tgggaagacg tggttaaaaa
     4921 taaaggcggc tggaatacct taatctggta cggcggtatt atcggcttaa gctccttatt
     4981 atcgaaagtt aaatttttcg aatggttagc tgaagtcttt aaaaataacc tggcatttga
     5041 tggtcacggt aacgttgctt tcttcgttat tattttcctc agcatcatcg tgcgttattt
     5101 cttcgcttcc ggtagtgcct atatcgttgc tatgttacct gtatttgcca tgctggcgaa
     5161 cgtctccggc gcaccgttaa tgttaaccgc gctggcactg ttgttctcca actcctatgg
     5221 cggcatggtt actcactatg gcggcgcggc aggtccggtc atctttggcg tgggttacaa
     5281 cgatattaaa tcctggtggt tggtcggtgc ggtactgacg atattaacct tcttggtgca
     5341 tatcaccctc ggcgtgtggt ggtggaatat gctgatcggc tggaacatgc tgtaaatata
     5401 ctcgtcatac ttcaagttgc atgtgctgcg tctgcgttcg ctcaccccag tcacttactt
     5461 atgtaagctc ctggggattc actcacttgt cgccttcctg caactcgaat tatttagagt
     5521 atatccattt attatctttc tgcgcacttc acggtgcgca gatatctgga gcatttgatg
     5581 atcaagttat ctgaaaaagg cgtgtttctc gccagtaata acgaaataat tgccgaagaa
     5641 catttcaccg gcgaaattaa aaaagaagaa gcaaaaaaag gcactattgc ctggtctatt
     5701 ctctcttctc ataatacgtc cggaaatatg gataaactta aaattaagtt tgattcatta
     5761 gcctctcacg atattacctt tgttggtatt gtacagaccg ctaaagcgtc cggaatggaa
     5821 cgtttcccgc tgccgtatgt gctgaccaac tgccataact cactctgcgc cgtcggcggc
     5881 accattaacg gtgatgacca tgtttttggt ttatcggcag ctcagcgtta tggcggtatt
     5941 tttgtgcctc cgcatattgc ggtcatccat caatatatgc gtgagatgat ggctggcggg
     6001 ggcaaaatga tcctcgggtc agacagccac acccgttacg gtgcattagg gacaatggca
     6061 gtcggtgagg gtggcggtga gttggtaaaa cagctgctta atgacacctg ggatatcgac
     6121 tatccgggcg tggttgcggt gcatctgacc ggaaaacctg cgccgtatgt ggggccacag
     6181 gatgtggcgc tggctatcat tggcgcggtg ttcaaaaacg gttacgtcaa aaacaaagtc
     6241 atggagttcg ttggaccggg cgttagcgcg ctctctaccg atttccgtaa cagcgttgac
     6301 gtgatgacca cggaaaccac ctgtttaagc tccgtctggc aaactgatga agaagtgcat
     6361 aactggctgg cgctgcacgg tcgcggtcag gattactgcc agcttaaccc tcaaccgatg
     6421 gcgtactacg atggctgcat cagcgttgat ttaagcgcca tcaaaccaat gattgcgctg
     6481 ccgttccacc cgagcaacgt gtatgaaatc gacacactga accagaacct gaccgacatt
     6541 ctgcgtgaga ttgaaattga gtccgaacgc gtggcgcacg gtaaagccaa actctcgctg
     6601 ctggataaag tggaaaatgg tcgcctgaaa gtgcagcagg ggattatcgc gggctgttct
     6661 ggcggtaact acgaaaacgt catcgcggcg gcgaatgcac tgcgcggtca atcctgtggc
     6721 aatgacacct tctcgctggc ggtttacccg tcatcacagc cggtgtttat ggatctcgcc
     6781 aaaaaaggtg tggtagcaga tttgattggc gcaggcgcaa tcatcagaac cgcattctgt
     6841 ggcccatgct ttggcgcggg cgatacgcca atcaacaacg gtttgagtat tcgccacacc
     6901 acgcgtaact tcccgaaccg cgaaggctct aagccagcca atgggcagat gtcagcggtg
     6961 gcgttgatgg acgctcgttc tatcgctgcg actgcggcaa acggtggcta tttaacctct
     7021 gccagcgaac ttgattgctg ggacaacgtg ccggagtacg ccttcgatgt aacgccgtat
     7081 aaaaaccgtg tttatcaggg ctttgtgaaa ggggcaactc agcaaccgct gatttacgga
     7141 ccgaacatta aagactggcc ggaattgggt gcgctgactg acaatatcgt cctgaaagtg
     7201 tgctcgaaga tcctcgacga agtcaccacc accgacgaac tgattccttc cggtgaaacc
     7261 tcttcttatc gttcaaatcc gattggtctg gcggggaatg tcagcgagct gacagaggtg
     7321 tttgcgcgca ttaagcagat tgctggtcag gagcatattg atccgctgca aactgaaatt
     7381 ggcagcatgg tctatgcggt gaaaccaggc gatggttctg cgcgtgaaca ggcggcgagc
     7441 tgccagcgtg tgattggcgg tctggcgaat attgccgagg agtacgcgac taaacgctac
     7501 cgttctaacg tcatcaactg ggggatgtta ccgctgcaga tggcggaagt gccaaccttt
     7561 gaagtggggg attacattta catccctggc attaaagcgg cgctggataa tccgggtacg
     7621 acgtttaaag gttatgtgat ccatgaagat gcgccggtaa cggaaattac gctttacatg
     7681 gaaagcctga cggctgaaga gcgcgagatt atcaaggcgg gtagtttgat taacttcaat
     7741 aaaaaccgtc agatgtaaaa agcgccatgt gaatgtaggt cgcattcggc acttattgtc
     7801 ggatgcgatg ctggcgcatc ttatccgacc tacaaatcgc atcgaaccgt aggccggata
     7861 aggcgtttac gccgcatccg gcaaatagtt aatagctctt acttcttctc ctctgcaacc
     7921 actttgctgc ccacgccgcg gttattgtat tcccacatgc ggttgtagtt agtgtcattc
     7981 agattgcgct gtatttcgtc gttatcatct acgctgccgg tattacccgc aaacggacga
     8041 ttagagatca ccgcatcggc ccacggttta gccgtgttaa aaccttcgtt gatggcgcta
     8101 tcacggatca ccacctgacc gttggtatta gcatcaacat ccagcgagcg gcccagttgc
     8161 gccacgccat caccggaagc attgaaacgg ctgtttacgg cgaggaaacc gtaatagatg
     8221 ttagacagcg tagccggtgc aaacacatat gcttcttgct gggtacgtga gttcaccacg
     8281 cggaattcgg tgttatcgaa caccactgcg ccgcgaccag aaacgatatc cacatcccct
     8341 tcaatatagc tgttggtcac cagcgtacgc ggctgacggt tggtttccag acggttctgc
     8401 acaccgctgt tggtgacaaa gaaggtgttc tgacgaccga gaatgttaac gttgttaatc
     8461 tgtacttggt caccgtcagt acgcagtgct accgccggat ggttacctgc atctacgcta
     8521 tcgcccagcg tgttttcgat ggtcagattt tgcagttgca gtccattgtt ttgtgaccag
     8581 aagaccgcag aacacagaac accgatactg tcgctgcgtt tgctctggca gctatcgtac
     8641 atataccacg ctggtttacc tggcatatat ttgccgcgcg ggttgacgtc gtgacgccag
     8701 tcggcagggc tcatgccccc atcaagggaa agcccaatct tcacatcaat cggtttttca
     8761 cctgtaccgt acagagtgat tccacctgga gcagcaggga catacaccgt tccctgatac
     8821 tcaccaggca tcacggcaat atactggcgc ttgttggtgc gcttgataat tgccgcatct
     8881 accgccgcct gaatcgtggt atgcgttaca ccttgagtac ccgccgggcc gacaacaaag
     8941 tcaggttgcg caggcagggt tatcggggac ggattccacg ctgcagcacc tggtgtcagg
     9001 gatgcaaaat agtgttgcgc atcgaaattc agcgcttctt ttgccgacag aatcgggcga
     9061 gaagaggtac caggcgcagt ttgatcagaa ggacgttgat cgggtggtgt tgagctacag
     9121 gcggtcagcg tcacgccaaa agccaatgcc agcgccagac gggaaactga aaatgtgttc
     9181 acaggttgct ccgggctatg aaatagaaaa atgaatccgt tgaagcctgc ttttttatac
     9241 taagttggca ttataaaaaa gcattgctta tcaatttgtt gcaacgaaca ggtcattatc
     9301 agtcaaaata aaatcattat ttgatttcaa ttttgtccca ctccctgcct ctgtcatcac
     9361 gatactgtga tgccatggtg tccgacttgt gcccgagaag atgttgagca aacttatcgc
     9421 ttatctgctt ctcatagagt cttgcagaca aactgcgcaa ctcgtgaaag gtaggtggat
     9481 ccccttcgaa ggaaagacct gatgcttttc gtgcgcgcat aaaatacctt gatactgtgc
     9541 cggatgaaag cggttcacga cgagtagatg caattatggt ttctccgcca agaatctctt
     9601 tgcatttatc aagtgtttcc ttcattgata tcccgagagc atcaacatgc aatgttgtag
     9661 ggatggcaat ttttacgcct gttttgcttt gctcgacata aagatatcca tctacgatat
     9721 cagaccactt catttcgcat aaatcaccaa ctcgttgccc ggtaacaaca gccagttcca
     9781 ttgcaagtct gagccaacat ggtgatgatt ctgctgcttg ataaattttc aggtattcgt
     9841 cagccgtaag tcttgatctc cttacctctg attttgctgc gcgagtggca gcgaccgggt
     9901 ttgttgttat atggccttca gctattgcct ctcggaatgc atcgctcagt gttgatctga
     9961 ttaacttggc tgatgccgcc ttgccctcgt ctatgtatcc attgagcatt gccgcaattt
    10021 cttttgtggt gatgtcttca agtggagcat caggcagccc cctccttatt gctttaattt
    10081 tgctcatgta atttatgagt gtcttctgct tgattcctct gctggcgagg attttttcgt
    10141 agcgatcaag ccatgaatgt aacgtaacag aattatcact gttgattctc gctgtcagag
    10201 gcttgtgttt gtgtcctgaa aataactcaa tgttggcctg tatagcttca gt
//
LOCUS       DAZSNM010000148        10053 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZSNM010000148 DAZSNM010000000
VERSION     DAZSNM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10053
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.148"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..445
                     /gene="mxiC"
                     /locus_tag="ACHW42_003023"
     CDS             <1..445
                     /gene="mxiC"
                     /locus_tag="ACHW42_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR8944743.1"
                     /translation="NHRHTIVNFVEQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIR
                     TSDVIFMKKLESSSLLKDGSLSAEQLLLTLLYIFQYPSESEQILTSVIEVSRASHEDS
                     VVYQTYLSSVNESPHDIFKSESEREIAINILRELVTSAYKKELSR"
     gene            458..2518
                     /gene="mxiA"
                     /locus_tag="ACHW42_003024"
                     /gene_synonym="virH"
     CDS             458..2518
                     /gene="mxiA"
                     /locus_tag="ACHW42_003024"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFR8944744.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            2531..2932
                     /gene="spa15"
                     /locus_tag="ACHW42_003025"
                     /gene_synonym="spaK"
     CDS             2531..2932
                     /gene="spa15"
                     /locus_tag="ACHW42_003025"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFR8944745.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            2936..4228
                     /gene="spaL"
                     /locus_tag="ACHW42_003026"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             2936..4228
                     /gene="spaL"
                     /locus_tag="ACHW42_003026"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFR8944746.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            4309..4659
                     /gene="spa13"
                     /locus_tag="ACHW42_003027"
                     /gene_synonym="spaM"
     CDS             4309..4659
                     /gene="spa13"
                     /locus_tag="ACHW42_003027"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HFR8944747.1"
                     /translation="MYRDVEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVK
                     KHIIQYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLI
                     LDELSQEDMKYGIR"
     gene            4646..5524
                     /gene="spa32"
                     /locus_tag="ACHW42_003028"
     CDS             4646..5524
                     /gene="spa32"
                     /locus_tag="ACHW42_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HFR8944748.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            5518..6399
                     /gene="spa33"
                     /locus_tag="ACHW42_003029"
                     /gene_synonym="spaO"
     CDS             5518..6399
                     /gene="spa33"
                     /locus_tag="ACHW42_003029"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HFR8944749.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            6404..7054
                     /gene="spa24"
                     /locus_tag="ACHW42_003030"
                     /gene_synonym="spaP"
     CDS             6404..7054
                     /gene="spa24"
                     /locus_tag="ACHW42_003030"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HFR8944750.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            7069..7329
                     /gene="spaQ"
                     /locus_tag="ACHW42_003031"
     CDS             7069..7329
                     /gene="spaQ"
                     /locus_tag="ACHW42_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HFR8944751.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            7329..8099
                     /gene="spa29"
                     /locus_tag="ACHW42_003032"
                     /gene_synonym="spaR"
     CDS             7329..8099
                     /gene="spa29"
                     /locus_tag="ACHW42_003032"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFR8944752.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            8106..9134
                     /gene="spa40"
                     /locus_tag="ACHW42_003033"
                     /gene_synonym="spaS"
     CDS             8106..9134
                     /gene="spa40"
                     /locus_tag="ACHW42_003033"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HFR8944753.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            9156..9389
                     /locus_tag="ACHW42_003034"
     CDS             9156..9389
                     /locus_tag="ACHW42_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator YmgB/AriR"
                     /protein_id="HFR8944754.1"
                     /translation="MGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKDIIN
                     VLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT         3146 a         1437 c         1968 g         3502 t
ORIGIN      
        1 taatcacaga catacaattg tgaattttgt agagcagtca ctgattgtag acatggattc
       61 tgagaaaccg agctgtaatg cttatgagtt tggttttgtg ttatctaaat taattgcaat
      121 taagatgatt agaacttcag acgtaatttt tatgaagaaa ctggaatcct caagcttgct
      181 aaaagatggc agtttaagtg cagagcagct attgctaact ttattatata tttttcaata
      241 tccaagtgaa agtgagcaaa ttcttacttc tgttatagaa gtatcacgag ccagtcatga
      301 ggattctgta gtgtatcaaa catatctatc ttctgttaat gaaagtcctc atgatatatt
      361 taaaagtgaa agtgaaagag aaattgcgat caatattcta cgagagcttg tcacaagtgc
      421 atacaagaaa gagctttcta gataacagga gataaaagtg atccagtctt ttcttaagca
      481 agtaagtact aagcctgaat taattatatt agtgcttatg gttatgatca ttgctatgtt
      541 gatcatacca ttacctacat accttgttga ttttttaata gggcttaata ttgtacttgc
      601 tattctcgtt tttatgggat cattttatat tgaaagaata ttgagttttt ctacattccc
      661 ttcggttttg ttgataacga cattatttcg tctcgcttta tcaataagta ctagtcgact
      721 aattttagtt gatgcggatg cgggtaaaat tattactaca tttggccagt ttgtcattgg
      781 tgatagtctg gctgtaggtt ttgttatttt ttcaatagta actgttgtgc agttcattgt
      841 tataacaaaa ggatctgaaa gggttgcgga ggtagctgct cgcttttctc ttgacggtat
      901 gccggggaaa caaatgagca ttgatgcgga tttaaaggct ggaattattg atgccgcagg
      961 agctaaagaa agacgcagta ttctggaacg tgagagtcaa ttgtatggtt catttgatgg
     1021 agcaatgaag ttcattaaag gtgatgcaat tgctggcatc attattattt ttgtcaattt
     1081 aataggaggt atttctgttg gtatgagtca acatggaatg tccctatctg gtgcattatc
     1141 tacttatacc atactaacta ttggtgacgg attggtctct cagattcctg ctctgttaat
     1201 ttctataagt gcaggattta tcgttacaag agttaatggt gatagcgata atatgggacg
     1261 taacataatg tcccagatat ttgggaatcc ttttgttctc attgttacat cagctcttgc
     1321 cttggcgata gggatgttgc caggttttcc gttttttgtt tttttcctga tagcagttac
     1381 tttgacggct ttattctatt ataaaaaggt cgtagaaaaa gaaaaaagtc tgtctgagtc
     1441 tgattctagt ggctatactg gtacatttga tattgataat agccatgact catctttggc
     1501 aatgatagaa aatctggatg ctattagttc agaaaccgtt cctttaattt tattatttgc
     1561 cgaaaataag ataaatgcaa atgatatgga aggtcttatt gaaaggataa gaagtcagtt
     1621 ctttattgat tatggtgtca ggcttccaac tattttatat agaacaagca atgagcttaa
     1681 ggtcgatgat attgttttgt taataaatga ggtgcgtgcc gatagtttta atatatattt
     1741 tgacaaagtg tgtattacag atgaaaatgg agatatagat gctctaggta ttcctgttgt
     1801 ttcaacttca tataatgaac gtgttatttc ttgggtagac gtttcatata cagaaaatct
     1861 aactaatatt gatgctaaaa ttaaaagtgc tcaagatgaa ttttatcacc agttgtcaca
     1921 agctttatta aacaacataa atgagatttt tggtatacaa gaaacaaaaa atatgttaga
     1981 tcagtttgaa aatcggtatc ctgatctatt aaaggaagtc ttccgacatg tgactataca
     2041 gagaatttct gaggtattac aaagattgct tggagaaaat atttctgttc gcaatttaaa
     2101 acttattatg gagtctttgg cgctttgggc tcccagagaa aaagatgtca taacattagt
     2161 tgaacatgtc cgtgcatcac tttctaggta tatttgtagt aaaatagctg tttctggtga
     2221 gattaaagtt gtgatgcttt ccggatatat tgaggatgca ataagaaaag ggataaggca
     2281 aacctctggt ggctctttct tgaatatgga tatagaggtt tcggatgagg taatggaaac
     2341 tttagcacat gctttgagag aattgagaaa tgcaaaaaaa aatttcgttc ttttggtatc
     2401 agtagatata cgtaggtttg ttaaaagact tatagataac agatttaaga gtatactcgt
     2461 tatatcgtat gctgagattg atgaagcata taccattaat gtattaaaga ctatttagtg
     2521 aggtttaaat atgagtaaca ttaatttagt tcaattagtt agagatagtc ttttcacgat
     2581 tggttgtcct ccatcaatta ttacagattt agacagccat tcagcgatta ccatttcttt
     2641 ggatagtatg cctgctatta atatagctct tgtcaatgag caggttatgt tatgggctaa
     2701 ttttgatgca ccaagtgatg taaaacttca gtcatctgct tataacatat taaatttaat
     2761 gttaatgaac tttagttata gcattaatga gctggtagag cttcatcgtt ctgatgagta
     2821 cttacagtta agagttgtta ttaaagatga ctacgttcac gatgggattg tttttgcaga
     2881 gattctgcat gagttttatc agaggatgga aatcttaaat ggggtcttat aatcaatgag
     2941 ctatacaaaa ttgctcactc aattatcttt tcctaataga atctcggggc caatcttgga
     3001 aacaagtctt agcgatgttt cgattggtga gatttgtaac attcaggctg gaattgaaag
     3061 taatgaaatt gttgcaagag ctcaggttgt aggatttcat gatgaaaaaa caatattaag
     3121 cttgattgga aattctcgtg gactttcacg gcaaacgttg attaagccca ctgcccagtt
     3181 tcttcatacg caagtcggcc gtggattatt gggagcagta gtcaatcctt taggggaggt
     3241 tactgataag tttgctgtta cagataacag tgaaattctt tatcgacctg tagataatgc
     3301 tcctccgcta tatagtgaaa gggctgcaat tgagaagcct tttttaacag gtattaaggt
     3361 tattgattct ttactcacgt gtggtgaagg acagcgaatg gggatttttg cgtcagctgg
     3421 ttgtggcaaa acttttctca tgaatatgct cattgaacat agtggtgctg atatatatgt
     3481 tattgggtta attggtgagc gaggtcgaga ggttactgaa acggttgatt atttgaaaaa
     3541 ctctgagaaa aaaagcaggt gtgttttagt atatgcaact tcggattact cttcggttga
     3601 tcgttgtaat gctgcatata tagccactgc tatagccgaa ttttttagga ctgaaggaca
     3661 taaagtagcg ctttttattg attcattaac aaggtatgcc agagcattac gtgatgtggc
     3721 cttagccgct ggagaatcac ctgccagaag aggctatccg gtttcggttt ttgatagctt
     3781 acccagactt cttgaaaggc caggaaagtt aaaggcaggt ggctctatta ctgcatttta
     3841 cactgttctt ttggaggatg atgattttgc tgatccatta gctgaagagg taagatccat
     3901 tttagatgga catatatatt tgagcagaaa tctagcccaa aaaggacaat ttcctgcaat
     3961 tgattcctta aaaagtataa gcagggtatt tacacaggtt gttgatgaaa aacatcgtat
     4021 tatggccgct gcatttcggg agctactttc tgaaatagaa gagctaagga caattattga
     4081 ctttggtgaa tacaaaccgg gggagaatgc ctctcaggat aaaatctaca acaaaatatc
     4141 tgttgttgag agttttctga agcaagatta tcgactcggt tttacttatg agcagacaat
     4201 ggagcttatt ggtgaaacaa ttagataagg ttttaaaaat caaagataaa tatcaacggt
     4261 ctgttaaatt aattgaagca cacatattaa cactaaaaaa aaaactcttt gtatcgagat
     4321 gtggaggcat tagataaaag gattatatac ttcctgcaac tggagaatga cttagaacct
     4381 gtaggcgctc agagtgttag tcagcttttc aatacaagac gtaagatcgc tatagtaaaa
     4441 aagcatatta tccaatatca gtctgaacga attcttctaa agggaagaat tgaagaaatt
     4501 caaaaagata ttgatgaggc aaatgcttct aaaagaaaac tgcttcataa agagagtaaa
     4561 atatgtaaac ggattggctt gattaaaaga aataactttg ccaaacagtt gatacttgat
     4621 gagttatcac aagaggatat gaagtatggc attagataat ataaacctaa attttagtag
     4681 tgacaaacag atagaaaaat gtgagaaact atcttcaata gataatattg atagtctcgt
     4741 tttgaagaag aagaggaagg ttgaaattcc tgagtactct ttaattgcat ctaactattt
     4801 cactattgat aagcactttg aacataagca tgataaagga gaaatttata gtggcattaa
     4861 aaatgcgttc gaacttagaa acgaacgagc gacatattct gatattccag aatcaatggc
     4921 cattaaagaa aatattttga taccagatca agatatcaaa gcaagggaaa aaataaatat
     4981 cggcgatatg agggggatct tttcatataa taagagtgga aatgcagaca agaacttcga
     5041 aagaagtcat acttcttctg taaaccctga taatctgcta gaatctgata atagaaatgg
     5101 tcaaattggt ttaaaaaatc atagcttgtc tattgataag aatattgctg acatcatttc
     5161 tttactaaat ggaagtgttg ctaaatcatt tgagctgcct gtaatgaata aaaatactgc
     5221 agacataacc ccatccatgt cattgcaaga aaaatcaata gttgaaaatg ataaaaatgt
     5281 ttttcaaaaa aatagtgaaa tgacttacca ctttaaacag tggggggctg gacattctgt
     5341 tagtatttca gtggagtctg gttcttttgt tctaaaaccg tcagatcaat ttgtaggaaa
     5401 taaacttgac ttaattttga aacaagatgc tgagggtaat tacagatttg atagcagtca
     5461 acataataag gggaataaaa ataatagtac aggatataat gaacagagtg aagaagaatg
     5521 ctaagaatta aacattttga cgctaacgaa aaactacaga ttttatatgc aaagcaactc
     5581 tgcgagcgtt tttcgattca gacattcaaa aataaattta caggcagtga aagtttagtc
     5641 actcttactt ccgtgtgtgg ggattgggta attcgtattg atacattatc ttttttgaaa
     5701 aaaaaatacg aggtattttc aggattttct acacaagaat ctttactgca tttatcaaaa
     5761 tgtgtcttta tagagtcgtc atctgtattt tcgattccag aactgtctga taagattact
     5821 ttccggatca cgaatgaaat ccagtatgca actactggga gtcatttatg ctgtttttcc
     5881 tcttctttag gtattattta ttttgacaag atgccggtat tacgtaatca agtttctctt
     5941 gactcattgc atcatctttt agagttttgc ttaggttcat ctaatgtaag gctggctact
     6001 ttaaaaagaa ttcgcactgg tgatataatc atagttcaga aactttataa tttattattg
     6061 tgtaatcaag ttattattgg ggattatatt gtgaatgata ataatgaggc aaaaattaat
     6121 ctgtcagaaa gtaatggtga gtcagaacac acagaagttt ctttggcatt attcaattat
     6181 gatgatatca atgtaaaagt ggactttatt cttttagaaa aaaatatgac aatcaatgaa
     6241 ctaaaaatgt atgtagaaaa cgaattattc aagtttcccg atgacatagt taaacatgta
     6301 aatattaaag taaatggttc tttggttggg catggggaac ttgtttctat tgaggatggt
     6361 tatggtatcg agattagttc ttggatggta aaggagtaat ctcatgctga gtgacatgtc
     6421 cctcatcgct acactttctt tttttacgct attaccattt ttagttgctg ctggtacctg
     6481 ttatataaag ttttctattg tttttgtaat ggtacgaaat gcacttggtt tgcagcaggt
     6541 tccatcaaat atgactctca atggtattgc attgattatg gctttgttcg tgatgaaacc
     6601 cattattgaa gctggttatg aaaattatct aaatggccct caaaaatttg atacaatatc
     6661 tgatattgtc cgtttttctg attctggctt gatggaatat aagcaatact tgaaaaagca
     6721 tacagatctt gagttagcac gttttttcca aaggtctgaa gaagaaaatg ctgatttaaa
     6781 gagtgcagag aataatgatt attcattgtt ttccctcttg cctgcatatg cgctaagtga
     6841 aatcaaagat gcgttcaaaa taggttttta tttatatttg ccatttgttg ttgttgatct
     6901 tgttatttca agcattttac ttgcacttgg tatgatgatg atgagtccaa tcacaatatc
     6961 agtaccaata aaacttgtat tatttgttgc gcttgatggg tggggtatcc tgtctaaggc
     7021 cttgattgaa caatatatca atattcctgc ttagatgatg gaggtaatgt gtctgatata
     7081 gtttatatgg gtaataaggc tctttattta atccttatct tttccttatg gccagtaggt
     7141 atagctacgg ttattggatt atctattggt ttgttacaga cagtgactca actccaagag
     7201 cagacacttc cttttggtat aaagcttata ggtgtctcaa tatctttgct acttctttct
     7261 ggatggtatg gtgaggtttt attgtctttt tgtcatgaaa taatgttttt aattaagagt
     7321 ggggtttgat ggacatttca agctggttcg aaagtattca tgtgttttta atactcctga
     7381 acggcgtttt ttttagattg gctccattat ttttctttct tccattttta aataacggta
     7441 taatttctcc atctattaga atacctgtga tttttcttgt tgcatcagga ttaattactt
     7501 ctggtaaggt agacataggt tcttctgttt ttgaacatgt ttatttcctt atgttcaagg
     7561 aaataattgt tggcctcctt ctctcttttt gcttgtctct tcccttttgg atatttcatg
     7621 ctgttggtag cattattgac aaccagcgtg gggcaacgct tagtagttca attgatcctg
     7681 ccaatggtgt tgatacgtct gagttggcaa aatttttcaa tcttttttct gcagttgtat
     7741 ttctatacag tggtggtatg gtctttattt tagaatccat acaattgtct tataatatat
     7801 gcccgttatt ttctcaatgt tctttccgtg tctcaaatat cttaacattt ctgactttat
     7861 tggcaagtca ggctgttatt ttagccagtc ctgttatgat agtattgtta ctatcagaag
     7921 tattacttgg tgtattatcg agatttgctc cgcagatgaa tgctttttcc gtatcattaa
     7981 ctattaaaag tttacttgca atatttatta ttttcatctg ttcttctact atttactttt
     8041 ctaaagttca atttttcctc ggtgaacata aatttttcac aaatctattt gttagataaa
     8101 atattatggc aaataaaaca gaaaagccga cacctaaaaa actaaaggat gccgcaaaaa
     8161 aaggacagtc atttaaattt aaggatttaa cgactgttgt tattattctg gtagggacat
     8221 ttactataat atcattcttt tccttaagtg atgtaatgct cttatacaga tatgtaataa
     8281 ttaatgactt cgaaattaat gagggtaaat acttttttgc agtggttatt gtctttttta
     8341 agataattgg cttcccactt tttttctgtg ttctttcggc tgtgttgcca acattggttc
     8401 aaacaaagtt tgttcttgcg actaaagcta tcaagattga tttttcagta ttaaaccctg
     8461 ttaaagggtt aaaaaaaata tttagtataa agacaataaa agaatttttc aaaagcattc
     8521 tgcttcttat tattctagca ttaacaacct atttcttttg gattaatgac cgaaaaataa
     8581 ttttttctca ggtgttttct agtgttgatg gcttatatct tatttggggg aggctgttta
     8641 aggatataat attatttttc ttggcatttt ctattcttgt tattattctt gactttgtga
     8701 ttgagttcat tttatacatg aaagatatga tgatggataa acaggagata aaaagagaat
     8761 atatagagca agagggacac tttgagacaa agtcgagaag gcgtgagttg catatcgaga
     8821 ttctttcaga gcagactaaa tctgatatac gtaattcaaa attagtggta atgaacccga
     8881 ctcatattgc aattggtatt tattttaatc cagaaatagc gcctgcacct tttatttctc
     8941 tcattgaaac taaccagtgt gccttggctg tcagaaaata tgcaaatgaa gttggtatac
     9001 cgactgtgcg tgatgtgaaa ttagctagaa aactatataa aacacataca aaatatagtt
     9061 ttgttgattt tgaacacttg gatgaagtcc tacgtcttat tgtttggctt gagcaggttg
     9121 aaaacactca ttaataagta aaggagatgt gttatatggg agttaatttc tgtaataaaa
     9181 taggtattga tcagtctgaa tttgaaatag aatcttccat cattaactcc attgctaatg
     9241 aggtattgaa cccaatatct ttccttagca ataaggatat aataaatgtt ttactcagaa
     9301 agatttcatc tgaatgtgac ctggttagaa aagacattta tcgttgtgct ctggagttgg
     9361 tcgttgaaaa aacacctgat gatttataat cattcataat aactcttttg ctgatataaa
     9421 tatttttgtt gaagatattt atgtagtcac aggatcaatt gtagaaaatt gttatgtttt
     9481 tgttaggaac tgcactaaat aatatatgat aagacagcaa aaacgactga ccataatcct
     9541 tcttttatta ggagttgata agagagacta ttcatcctgt aatgttaaaa ctctgttata
     9601 tagtattcgc gattatgcaa agtctgtaaa tgatcatgag atactaaccg agagtaatcg
     9661 tttattatct cattgcattt cagatagtaa cggagctttc tttaaaagta gtaaatacgt
     9721 tccccttaag tacttacgta aaaggagaat agcacgaaag ataccaaatg attagcactt
     9781 tgctagtaag cttgctggta aactatattg accgttgggt ttgttttgag actgtaattt
     9841 aaattgtgta attgcctgtt tttgatatgt ttactccaac aacggagaca ggtaaattat
     9901 ggacgaaaag aagcttaaag cccttgcggc tgaactggct aaaggcctca aaactgaggc
     9961 cgacctcaat cagttttccc gtatgctgac gaaatatccc ggctcttaaa acgatagtgg
    10021 ctcaggcttt catttttcag cgttccccag aag
//
LOCUS       DAZSNM010000149         9999 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZSNM010000149 DAZSNM010000000
VERSION     DAZSNM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9999
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.149"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..315
                     /locus_tag="ACHW42_003035"
     CDS             <1..315
                     /locus_tag="ACHW42_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR8944755.1"
                     /translation="GLLKLKVKTNSSSSVLVGPLFGSSAPAPVTVGNTAAPAVAAPAP
                     VPVKKSEPMLNDTESYFNTAIKNAVAKGDVDKALKLLDEAERLGSTSARSTFISSVKG
                     KG"
     gene            544..2124
                     /locus_tag="ACHW42_003036"
                     /pseudo
     CDS             544..2124
                     /locus_tag="ACHW42_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            2347..2844
                     /gene="ubiC"
                     /locus_tag="ACHW42_003037"
     CDS             2347..2844
                     /gene="ubiC"
                     /locus_tag="ACHW42_003037"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR8944756.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            2857..3729
                     /gene="ubiA"
                     /locus_tag="ACHW42_003038"
     CDS             2857..3729
                     /gene="ubiA"
                     /locus_tag="ACHW42_003038"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR8944757.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(3884..6307)
                     /gene="plsB"
                     /locus_tag="ACHW42_003039"
     CDS             complement(3884..6307)
                     /gene="plsB"
                     /locus_tag="ACHW42_003039"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR8944758.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            6478..6846
                     /gene="dgkA"
                     /locus_tag="ACHW42_003040"
     CDS             6478..6846
                     /gene="dgkA"
                     /locus_tag="ACHW42_003040"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR8944759.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            6956..7564
                     /gene="lexA"
                     /locus_tag="ACHW42_003041"
     CDS             6956..7564
                     /gene="lexA"
                     /locus_tag="ACHW42_003041"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR8944760.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            7637..8962
                     /gene="dinF"
                     /locus_tag="ACHW42_003042"
     CDS             7637..8962
                     /gene="dinF"
                     /locus_tag="ACHW42_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR8944761.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            9078..9287
                     /locus_tag="ACHW42_003043"
     CDS             9078..9287
                     /locus_tag="ACHW42_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR8944762.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(9329..9844)
                     /gene="zur"
                     /locus_tag="ACHW42_003044"
     CDS             complement(9329..9844)
                     /gene="zur"
                     /locus_tag="ACHW42_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR8944763.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         2426 a         2296 c         2585 g         2692 t
ORIGIN      
        1 ggcttactga aactgaaagt caaaacgaac tccagctcca gcgtgttggt aggaccgcta
       61 tttggttctt ccgctccagc tccggttacg gtaggcaaca cggcggcacc agctgtggct
      121 gcacctgctc cggtgccggt gaagaaaagc gagccgatgc tcaacgacac ggaaagttat
      181 tttaataccg cgatcaaaaa cgctgtcgcg aaaggtgatg ttgataaggc gttaaaactg
      241 cttgatgaag ctgaacgcct gggatcgaca tctgcccgtt ccacctttat cagcagtgta
      301 aaaggcaagg ggtaattacg ccccacagtg ctgattttgc aacaactggt gcgtctcctg
      361 gcgcaccttt ttttatgctt ccttcctggg atatgagcga ttttttatag tacttcactt
      421 cttcttcact aagaatgtcc attatttcaa tgcattatca gagattcttt tcctttcgcc
      481 ggtagtgtct ggacgctcag gctacttttc caggttattt tatttctgta ttacagaggt
      541 tttatgatag gtcatatcac aaattctggc ggcaataact ctttgatgaa acatgatgtg
      601 gtgcaaggaa ataatatagt agatcttgat ttactacgta atttcaatgg ggtgacaggt
      661 ttaaacagag attactttat ttatatcagt gatatatttt taaatataaa acaacggaac
      721 gaaaaaaatc atgcaataaa tatgttacgt gaagtctcaa ttagtaatga tactataagt
      781 gtaaaattct acagaaatga aaaaatagaa tgcgcttgta attttctgat ggataaagat
      841 gcgcaggggt atatcgacct gtctgatttt gatttaacaa attgtcattt taaaggtgac
      901 gttatttcta aggtgtcttt tttatcatca aatctacaac atgtaacttt cgaatgtaaa
      961 gaaattgaga attgcaattt tactaaggca acagttgata atgtcatatt taaatgtcga
     1021 cgtttacaca atgtgatttt tctcaaaacg agtggcgaat gtgttgattt tagccaaaat
     1081 attcttgata cagttgactt ctcgcagagt caacttggtc atagtaattt tcgcgaatgt
     1141 cagattagaa attcaaactt cgataattgt tatctttacg cttcgcactt caccagagca
     1201 gagtttctgt ctgccaaaga aatatcattt attaaatcga atttgacagc tgttatgttt
     1261 gatcatgtgc gaatgtcgac agggaatttt taagattgca ttacagaaca attggaatta
     1321 actattgatt attcagatat atttgggaat gaagatctcg atggttatat caataacatt
     1381 ataaaaatga ttgatacatt gccagataat gcaatgatat tgaaatccgt tctggcagta
     1441 aaactggtga tgcaattaaa aattctcaat attgttaata aaaactttat tgagaatatg
     1501 aagaaaacat ttagccattg tccttatata aaagatccaa ttatacgtag ttatatacat
     1561 tctggggaag atgacaagtt cgataatttt atgcgtcaac atcgattcag taaggtgaat
     1621 ttcgataccc aacagatgat ccattttatt aacagattta atatgaataa agggctggtt
     1681 gataaaaata acaatttttt tatccaactt atcgatcagg ccttacgatc aacggatgat
     1741 atgatcaaag caaatgcctg gtatctttat aaagagtgga ttcgtagtga tgatgtttca
     1801 cctatattta tagaaactga agaaaagtta agaaccttta acactaataa attaacacga
     1861 aacgataata tatttatcct gttctcctct gtcgatgatg ggcctgttat ggtggtaagc
     1921 tcccagcgct tacatgatat gttgaatcct acaaaagata ccaattggaa ttccacgtgt
     1981 atctataaat ccagacataa gatgttgcct attaatctta ctcaggaaac acttttcagc
     2041 tccaaatctc atggtaaata tgcgcttttc ccaattttta ctgcgagttg gcgagctact
     2101 cgtataaaga ataaaggtat ttaagtaaag gaaaacatca ccgttcctag catcatgggc
     2161 ggtgatgccc ctatggttgc cctcgccagc acgggcatcg gcaaagcgta aggttcaaca
     2221 gcgttttacc acttcatgcg attgttgcgt ttttgttgcg cattagatca cttaatttgc
     2281 tttacatctc ccgtaaacac ttttctgcga tacaatgcct ttacgttatg taacggagag
     2341 ttcggcatgt cacaccccgc gttaacgcaa ctgcgtgcgc tgcgctattt taaagagatc
     2401 cctgccctgg atccgcaact gctcgactgg ctgttgctgg aggattccat gacaaaacgt
     2461 tttgaacagc agggaaaaac ggtgagtgtg acgatgatcc gcgaggggtt tgtcgagcag
     2521 aatgaaatcc ccgaagaact gccgctgctg ccgaaagagt ctcgttactg gttacgtgaa
     2581 attttgttat gtgccgatgg tgaaccgtgg cttgccggtc gtaccgtcgt tcctgtatca
     2641 acattaagcg ggccggaact agcgttacaa aaattgggta aaacgccgtt aggacgctat
     2701 ctgttcacat catcgacact aacccgggat tttatcgaga taggccgtga tgccggactg
     2761 tgggggcgac gttcccgcct gcgattaagc ggtaaaccgc tgttgctaac agaactgttt
     2821 ttaccggcgt caccgttgta ctaagaggaa aaaaatatgg agtggagtct gacgcagaat
     2881 aagctgctgg cgtttcatcg cttaatgcgt acggataagc caattggcgc gttactgctg
     2941 ctctggccaa cattatgggc gttgtgggtg gcgacaccgg gcgttcctca gctctggatc
     3001 atggcggtgt ttgtcgcggg tgtctggctg atgcgcgctg ctggttgcgt ggtgaatgat
     3061 tatgctgatc gcaagtttga tggtcatgtt aagcgcacgg cgaaccgacc acttcccagc
     3121 ggcgcggtaa cagagaaaga ggcgcgcgcg ctgtttgtag tgctggtact gatttcgttt
     3181 ttactggttc tgacgctgaa tacgatgacc attctgttgt cgattgccgc tctggcattg
     3241 gcgtgggtgt acccgtttat gaagcggtat acccatctcc cgcaagtggt gctgggcgcg
     3301 gcgtttggct ggtcgattcc aatggctttt gccgctgtga gtgagtcggt gccattgagt
     3361 tgctggttaa tgttcctcgc caatattctc tgggcggtgg cttacgacac gcagtatgcg
     3421 atggttgacc gcgatgatga tgtgaagatt ggcattaaat ccacggcaat cctgttcggc
     3481 caatacgata aattgattat tggtattttg cagattggcg tactggcact gatggcgatc
     3541 atcggtgagt taaatggctt aggctgggga tattactggt caattgtggt ggctggcgcg
     3601 ctgtttgttt atcaacaaaa actgattgcc aaccgcgagc gtgaagcctg ctttaaagca
     3661 tttatgaata ataactatgt tggtctggta ctgtttttag gactggcaat gagttactgg
     3721 catttctgat gatgtaaaaa agccggatga tcatccggct ttcttctggg ttgcctgatg
     3781 cgttgcgctt ctcaggccta cccggtgaat cgcaatttat tgaatttgca ggtcatggta
     3841 ggccggataa ggcgttttcg ccgcatccgg caattctctc tgattaccct tcgccctgcg
     3901 tcgcactctc aatcgtcaaa cgcacgtctg atgtaatcaa ctccgccagc aactgataaa
     3961 ccttcatcgt ttctgctggt tcggcatcgc cgctatcgct gatataccct tcatcacgca
     4021 gcgtcagcac cagagaactg aacaccgcct tgtcgaagaa ctccggcgcg ttgatgccgt
     4081 gcagcacgga gagacgttgc gcaacggtgc ggctctcttt ctccagcgta ccgcggttga
     4141 tcgacgggtt ggcactcaac aaccagaagg tgatggcata acgttgcagc gtttcgcgcg
     4201 cgcctgcggc cagcagctgt agggtgcgag aatgcgccgg gttgatatgc aactcatcat
     4261 cttgcagggt aatcagcccc tgacgttgca tctcatttgc cagcgcatca ataacgtccg
     4321 gcaactcgtc gcgatcccag cgcaggaaca gctccgcttt cagcattggg taaagcacat
     4381 tgacgtgctc catcaatacg tcgcgggaga tgtggcgatg ctgggtgaca attgccgcca
     4441 tcagcgaagg cagcaccaac atatgcgcaa tgttgttgcg atagtaggtc atcagcaccg
     4501 cttgctcgcg tggcagaatg atgatgtcgc cgatagtgtc tttctcgact tcaaacttgt
     4561 tcatttgcag cgcgtgatcg ataagctcgc tggcgctggc tgaaggaacg gtagagtccg
     4621 tggagtaagg tacgttgcgc atcagatcca ggtagcagtt gagttgctcg gttaactgct
     4681 cgcgggtgag tgagcgctga cgtgatgcca gtagcgcagt acagcacagg ttcatggcgt
     4741 ttgccgcgcc tgcgttgtta atgcgtacca tcagatcggc agcaatatta ttgaccgtcg
     4801 gcgttaacca ggccggacgc accgcttcga tgggatcgat agattcacgc cagtctggta
     4861 cgtgctggtt aaggtaggtc atcaacggca ttggttcacc gaagttgacg taaccctgac
     4921 cgagattacg cagcttgctt aaaccgcgca gcatctgcgg caggctctct ttctctttcg
     4981 tcgcgccgcg cagttctttg gcgtaagtac ccacttccat gacgtgctca taaccgatat
     5041 agatcggaat cagcgtaatc ggacgcgtgc cgccacgcag catcgcctga atggtcatcg
     5101 acagcgtacc ggttttcgga tccagcaaac gccccgtgcg ggaacgaccg ccttccacga
     5161 agtactcgac ggaataacca cggctgaaca gttcgccgag atactcacgg aaaacggtgg
     5221 aataaagttt attgccttta aaagtacggc gaataaagaa cgcccccaga cggcggaaaa
     5281 tcggcccggc cggccagaag ttcaggttga tcccggcggc gatatgcggc ggcaccagtc
     5341 cctggtggta cagcacgtaa gaaagtagca ggtagtccat gtgactgcgg tggcaaggca
     5401 catacaccag ctcatgaccg tcgtgggcca gctggcgaac gcgctcggcg ttatggacgt
     5461 tgatgccctg ataaaatcgg ttccaggtga agcccagaat gcggtcagtc aggcggatca
     5521 tctcgtaaga gaaattcgcc gcaatctctt ccatcagcgc aatcgcgttc tgctgcgctt
     5581 tttcatggga gatttttttg ctgcgcgctt catcttctac cgctttggca atggcgcggg
     5641 aggcgagcag cttattaaac agatcctgac gagcaggcag acgtgggcct acggcagcca
     5701 gacgttgacg ggcaaagtgc atacgcgcca cgcgcgccag tttctgagcg atagttttat
     5761 ccgtgccgtg ttcatccgcc atacggcgca gcgacactga cggcgagaaa cgcacaaaac
     5821 tgtcgcgacc gagccacagt acagcgaaaa atttctgtac gccgttaagc atacgcagcg
     5881 gcgggttcac ttcgcctttt tcacgccccg gcgcgcgacc aaacatcacc gatactggca
     5941 ccatctgcac atccagattt gggttgctac ggtgcaaatc gagatagtcg tggaaaagct
     6001 taatagactc ttctttcggc gtgtaatagg tgaatacacg cggcccgccg tgaatgaaca
     6061 catagcgcgg cagtagcgtg ccgtcgattt ccagcggctc taacgggtca ggcaaatcat
     6121 gtgccagaca ctgggcgcgc aacgtcagca aatctgcttt cgagttgtac ggtaaaacgt
     6181 acataattgg acgagaggta tccagcccca gttccggggc aggatctgcc ggaatagact
     6241 tgctttttac caggatgctt aatggtaaat tcagtaattt gtagtaaatt cgtggccagc
     6301 cggacataaa cgatgtaaag cctctggtta ataatgcaaa tgcgcggcaa ggatagcaga
     6361 aagtcatggg aaattctgtg gtatccgctc atgtttcgcg cggcgttacg caaacccgaa
     6421 tcatcggatt taacggtaca ctgatattga cgctcataat gtaaaaaggt tctttcaatg
     6481 gccaataata caactggatt cacccgaatt atcaaagctg ctggctattc ctggaaaggt
     6541 ttacgcgctg catggatcaa cgaagcggca ttccgtcagg aaggcgtagc ggtattgttg
     6601 gcggtggtca tcgcctgctg gctggatgtg gacgcaatta cccgcgtgct gcttatcagc
     6661 tccgtgatgc tggtgatgat tgtggaaatc ctcaatagcg ccatcgaagc agtggttgac
     6721 cgaattggct ctgaatacca tgagctttcc ggacgcgcaa aagatatggg atccgccgcg
     6781 gtgctgattg ccattatcgt cgccgtgatt acctggtgca tcctgttatg gtcgcatttt
     6841 ggataaccct tccagatttc gataaatctc tggtttattg tgcagtttat ggttccaaaa
     6901 tcgccttttg ctgtatatac tcacagtata actgtatata cacccagggg gcggaatgaa
     6961 agcgttaacg gccaggcaac aagaggtgtt tgatctcatc cgtgatcaca tcagccagac
     7021 aggtatgccg ccgacgcgtg cggaaatcgc gcagcgtttg gggttccgtt ccccaaacgc
     7081 ggctgaagaa catctgaagg cgctggcacg caaaggcgtt attgaaattg tttccggcgc
     7141 atcacgcggg attcgtctgt tgcaggaaga ggaagaaggg ttgccgctgg taggtcgtgt
     7201 ggctgccggt gaaccgcttc tggcgcaaca gcatattgaa ggtcattatc aggtcgatcc
     7261 ttccttgttc aagccgaatg ctgatttcct gctgcgcgtc agcgggatgt cgatgaaaga
     7321 tatcggcatt atggatggcg acttgctggc agtgcataaa actcaggatg tacgtaacgg
     7381 tcaggtcgtt gtcgcacgta ttgatgacga agttaccgtt aagcgcctga aaaaacaggg
     7441 caataaagtc gaactgttgc cagaaaatag cgagtttaaa ccaattgtcg ttgaccttcg
     7501 tcagcagagc ttcaccattg aagggctggc ggttggggtt attcgcaacg gcgactggct
     7561 gtaacatatc tctgagaccg cgatgccgct tggcgtcgcg gtttgttttt catctctctt
     7621 catcaggctt gtctgcatgg cattcctcac ttcatctgat aaagcactct ggcatctcgc
     7681 cttacccatg attttctcca atatcaccgt tccgttgctg ggactggtcg atacggcggt
     7741 aattggtcat cttgatagtc cggtttattt gggcggcgtg gcggttggtg caacggcgac
     7801 cagctttctc tttatgctgt tgctgttttt acgcatgagc accaccgggc tgactgcgca
     7861 ggcttatggt gccaaaaatc ctcaggcatt agcccgtgcg ctggtgcaac cgttgctgtt
     7921 ggcgttgggg gccggggcgt taattgcgct gttgcgtacg ccgattatcg atctggcgct
     7981 gcatattgtt ggcggtagtg aagcggtcct ggaacaggcg cggcgctttc ttgaaatccg
     8041 ctggttaagc gctccggcgt cgctggcgaa tctggtatta ctcggttggt tactcggcgt
     8101 gcaatatgcc cgtgcgccag taattttgtt agtggtcggc aatatcctca acattgtgct
     8161 ggatgtctgg ctggtgatgg ggctgcatat gaacgtgcag ggcgcggcgc tggcgacggt
     8221 tattgcggaa tatgcaacat tgctgattgg tctgctaatg gtgcgtaaaa tcctcaaact
     8281 acgcggaatt tccggcgaaa tgctgaaaac tgcctggcga ggaaacttcc gtcgcttgct
     8341 ggcgcttaac cgcgatatca tgctgcgttc gctgttgttg caactctgtt tcggcgcgat
     8401 caccgtactt ggtgcgcgac tggggagtga cattatcgct gttaacgcgg ttctgatgac
     8461 gctactcacc tttaccgcct atgcgcttga tggttttgcc tacgcggttg aagcgcactc
     8521 cggtcaggca tacggtgcgc gcgacggtag ccagttgctg gatgtatggc gggcagcgtg
     8581 ccgccagtcg gggattgtag cgttactatt ttcggtggtt tatttgctgg caggggaaca
     8641 catcattgcg ttgctgacgt cgttaaccca gattcagcag ctggctgacc gctatcttat
     8701 ctggcaggtg attttgccgt tggttggcgt ctggtgttat ctgctggacg gcatgtttat
     8761 aggcgcaacg cgtgccgccg aaatgcgtaa cagtatggcg gtggccgccg caggttttgc
     8821 gctgacgctc cttacgctgc cgtggctggg taatcatggt ttgtggctgg cattaaccgt
     8881 ctttctggcg ttgcgcgggc tttctctggc ggctatctgg cggcgtcact ggcgcaacgg
     8941 tacctggttt gccgcaacgt gacggttaaa aattctgaat aaattatcct aagccaaatt
     9001 gctgactaca cttaatctca cgttcagaag aaaagtgaac gtactctcat tcacaaccta
     9061 acgatgaggt cttgattatg aataaagatg aagccggcgg taactggaaa cagtttaaag
     9121 gtaaagtgaa agagcaatgg ggcaaactga ccgatgatga tatgacgatc attgaaggta
     9181 aacgtgatca actggtcggt aaaatccagg aacgttatgg ttatcagaaa gatcaggcag
     9241 aaaaagaggt cgtggattgg gaaacccgca atgaatatcg ctggtaatta atccctcctg
     9301 cccgacgtgt acaaggatgt acgccctctt aacgcggttt ctttttcacc tgcacagagt
     9361 gatcatgctg gcactgttca ggatgacgac acgcttccac ttccacgcat gccgcacaga
     9421 gcccatgtgc ttcaatcaca ttatgccgca gggcaaaccc catttttgcc gccagcgtat
     9481 gcataatgtc ttccacgcct tctgcacact cttctttcac cgcgccgcag cgatcgcaaa
     9541 taaacatggc tgacgtatgc gtgggctggt cgaacagatg acagagcaca taactgttgg
     9601 tggattccac cttatgcaca aaaccttgct caagcagaaa atccagcgcg cgataaaccg
     9661 ttggcggctt ggcttgcggt tcagcttcgc gcagtaaatc aagcagatca taagcgctga
     9721 tagcgccatc ctgcagactc atcaggcgca acacttccag gcgctgtggg gtcaggcgca
     9781 cattacgctg cgcgcagatt ttttcagcct gcgctaataa ctcctgcgtt gtggtctttt
     9841 ccatcgggca cctcaaagtg ggggttaaga atgtccttac tttaccatgt tccaggaaaa
     9901 acaccgagaa cccttattgt tgccgtaatg ttgattttct gttttgtagg taaggtgttg
     9961 tgttgcctta tcgtaccatt atcaacacga taataatta
//
LOCUS       DAZSNM010000150         9913 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZSNM010000150 DAZSNM010000000
VERSION     DAZSNM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9913
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.150"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(46..2325)
                     /gene="maeB"
                     /locus_tag="ACHW42_003045"
     CDS             complement(46..2325)
                     /gene="maeB"
                     /locus_tag="ACHW42_003045"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR8944764.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2614..3564
                     /gene="tal"
                     /locus_tag="ACHW42_003046"
     CDS             2614..3564
                     /gene="tal"
                     /locus_tag="ACHW42_003046"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR8944765.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3561..5576
                     /gene="tkt"
                     /locus_tag="ACHW42_003047"
     CDS             3561..5576
                     /gene="tkt"
                     /locus_tag="ACHW42_003047"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR8944766.1"
                     /translation="MNQPRSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLW
                     NDFLKHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPG
                     HPEIGYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGC
                     LMEGISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHE
                     IDGHDPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALA
                     RQKLGWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSG
                     GLPKDWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTI
                     WKGSVSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARM
                     AALMKARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKL
                     AVERHNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEI
                     TLQAAEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYK
                     YVGLKGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5679..6722)
                     /locus_tag="ACHW42_003048"
     CDS             complement(5679..6722)
                     /locus_tag="ACHW42_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR8944767.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(6848..7423)
                     /gene="nudK"
                     /locus_tag="ACHW42_003049"
     CDS             complement(6848..7423)
                     /gene="nudK"
                     /locus_tag="ACHW42_003049"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFR8944768.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7491..9470)
                     /gene="aegA"
                     /locus_tag="ACHW42_003050"
     CDS             complement(7491..9470)
                     /gene="aegA"
                     /locus_tag="ACHW42_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR8944769.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9676..>9913
                     /locus_tag="ACHW42_003051"
     CDS             9676..>9913
                     /locus_tag="ACHW42_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HFR8944770.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALH"
BASE COUNT         2334 a         2699 c         2544 g         2336 t
ORIGIN      
        1 cgcttaccct gaatattcag ggtaagcgtg agagttaaga atgaattaca gcggttgggt
       61 ttgcgcttca accacggcca gcgccaccat attgacgata cgacgcaccg atgcgatcgg
      121 cgttaacacg tgaaccggtt tcgccacgcc catcagcacc ggaccgacag tcacaccttc
      181 cgagctggaa acgcgcagta agttgtaact aatgcgggca gcttccatgt tcggcatcac
      241 cagaatattg gcagaacctt tcaaagggct gtccggcata cggtcgttgc gaatcgcttc
      301 caccagcgct gcatcgccgt gcatttcacc atcaatcatc agatctggcg cacgatccct
      361 gaccagttcc agcgcctgac gcattttgct cgacgacggg cagtcagaag aaccaaagtt
      421 ggagtgcgac aacagagcaa cgcgcggctc aataccaaaa cgacggacag tttctgccgc
      481 catcaaggtg atctccgcca gctcttctgc gtctggttcg tcattgacgt aggtatcggc
      541 aataaaggtg ttaccactcg gcagcagcag cgcattcatt gcacctgcgg tgtgaacgcc
      601 atcgcgataa ccaaagacat ttttcaccac actaaaatgt tcatgataat cgcccaccgt
      661 accgcagatc attgcatcgg cttccccacg ctgaaccatg atcgcgccga tcactgtcgg
      721 gttactgatc agcgcacgct gcgcctgttc ctgagtgaca ccgcgacgct tcatgatctg
      781 gaagtattcg gtccagtact ctttaaagcg cggatcggat tcgttattga cgatttcaaa
      841 atcaacgccc gctttgatct gcaagcccag tttctgaatg cgcatttcga tcacgttcgg
      901 acgaccgata aggatcggtt tcgccagtcc cagcgttacc agttcctgag tcgcatgcag
      961 aacgcgcgcc tcttcccctt ccggcagaac tacgcgcttc ggcgctttgc gagcctggga
     1021 gaaaatcggc ttcataaaca ggttggtttt gtaaacgaac tcagtcagct tgtcgatgta
     1081 gacgtcgaaa tcagcaatcg gacgcgtcgc cacgcccgac tccatcgccg cttttgcgac
     1141 cgcaggagcg atcttaacga tcaagcgcgg atcaaacggt tttggaatga tgtattccgg
     1201 accaaagctc agatcctgaa cgccatacgc tgaagccacc acttcactct gttctgcatg
     1261 ggccagttct gcaatcgcgc gtaccgccgc cagtttcatc tcttcgttga tggcggttgc
     1321 gccaacgtcc agcgcgccac ggaagatgaa cgggaagcaa agaacgttgt tcacctggtt
     1381 cgggtagtcg gaacggccgg tacagatgat ggcatccgga cgcacttctt tcgccagcgg
     1441 cggcagaatt tccggttccg ggttcgccag cgccaggatc attggtgccc gagccatttt
     1501 cttcaccatt tcctgggtca gcactttcgg gccggaacag cccaggaaaa tatccgcacc
     1561 ttcaatcaca tcatcgaggg tacgtttgcc gtcatccacc accgcatatg cggctttggt
     1621 ttccgccata tttggctcac ggccctgata gataacgcct tttgaatcgc aaaccacgat
     1681 gttatgtttt tgcagcccca gcgctaccag caggttcata caggcgattg ctgcggcacc
     1741 cgcgccggaa accaccatcc gcacgtcgga gatgtttttc tccaccacgc gcaagccgtt
     1801 gaggatggcg gcagtgctga tgattgccgt gccgtgctga tcgtcgtgga ataccggaat
     1861 attcatccgc tcgcgcagtt tctgttcaat atagaaacat tctggcgctt taatgtcttc
     1921 gaggttgatg ccgccgaagg tcggttcgag cgcggcgacg acttcaataa atttgtccgg
     1981 gtcgagttca tcaacttcaa tgtcaaatac atcaatccca gcgaatttct taaatagaac
     2041 gcccttgcct tccatcaccg gtttgcctgc cagcgcgcca atgttgccta accccagcac
     2101 cgccgtaccg ttagagatca ccgcaaccag gttaccgcgc gcggtatatt tgtaggcttt
     2161 tagcgggtct ttttcgattt caagacaagg tgcggcaacg cctggtgagt aggccagcgc
     2221 cagatcgcgc tgcgttgcca gaggcttggt tggagaaacc tggattttcc ctggaactgg
     2281 aaattcatgg aaatcaagtg cactttgttt taactggtca tccatttggt tgttcctttc
     2341 acgtaacgtt cacaaataaa gtgtgtgggc aacagcccct gcccacaacg tggcgcacat
     2401 tattaccctg ccggagtcta cagactttga gcaagtccaa actctcacca ttaatataat
     2461 gttttggtaa taatcctata acactgatgt tacctgctta atccagcaat accatgcctg
     2521 tctgctatgc ttttttgatg cgtttagcga aatttctcag aagtgtgaat taacgcactc
     2581 atctaacact ttacttttca aggagtattt cctatgaacg agttagacgg catcaaacag
     2641 ttcaccactg tcgtggcaga cagctgcgat attgagtcca ttcgccatta tcatccccag
     2701 gatgccacca ctaatccttc gctgttactc aaggctgccg gattatcaca atatgagcat
     2761 ttaatagacg atgctatcgc ctggggtaaa aaaaatggca agacccagga acaacaggtg
     2821 gtcgcagcgt gtgacaaact ggcggtcaat ttcggtgctg aaattctcaa aatcgtaccc
     2881 ggttgcgtgt caacagaagt tgatgcacgc ctctcttttg ataaagaaaa gagtattgag
     2941 aaggcgcgcc atctggtgga cttgtatcag caacaaggcg ttgagaaatc acgcattttg
     3001 atcaaactgg cttcgacctg ggaaggaatt cgcgcggccg aagagctgga aaaagaaggt
     3061 attaactgca acctgacgct gctgttttct tttacccagg cgcgggcctg tgcggaagca
     3121 ggcgtttttc tgatttcgcc gtttgtaggg cgtatttatg actggtatca ggcacgcaag
     3181 ccgatggacc cgtatgtggt ggaagaagat ccgggcgtta aatcggtgcg caatatctac
     3241 gactactata agcaacacca ctatgaaacc attgtgatgg gcgcgagctt ccgtcgcacc
     3301 gaacaaatcc tcgccttaac cggctgcgat cgactgacta tcgcaccgaa tttgctgaag
     3361 gagctgcagg aaaaagtttc tccagtggta cgtaaattaa tcccgccttc tcagacgttc
     3421 ccacgcccag cacccatgag cgaagcggag ttccgttggg agcacaatca ggatgcgatg
     3481 gcggtagaaa aactgtctga aggcattcgt ctgttcgctg ttgatcaacg caaactggaa
     3541 gatcttcttg ccgccaaact ataaaccagc cacggagtcg aaaagacctt gccaatgcga
     3601 ttcgcgcact cagtatggat gctgtacaaa aagccaattc tggtcatccc ggcgcgccga
     3661 tgggcatggc tgatattgcc gaagtgctgt ggaacgattt tcttaaacat aatcctaccg
     3721 acccaacctg gtatgatcgc gaccgcttta ttctttccaa cggtcacgcg tcgatgctgc
     3781 tctacagttt gctacatctg accggttacg acctgccgct ggaagaactg aagaacttcc
     3841 gtcagttgca ttcgaaaacc ccaggccacc cggagattgg ctatacgcca ggcgttgaaa
     3901 ccaccaccgg cccgcttgga caaggtttgg cgaacgccgt cgggctggcg atagcagagc
     3961 gtacactggc ggcgcagttt aaccagccag accatgagat cgtcgatcac ttcacctatg
     4021 tgtttatggg cgacggctgc ctgatggaag gtatttccca cgaagtctgt tcgctggcag
     4081 gcacgctggg actaggcaag ctgattggtt tttacgatca caacggtatt tccatcgacg
     4141 gtgaaacaga aggctggttt accgacgata cggcaaaacg ttttgaagcc tatcactggc
     4201 atgtgatcca tgaaatcgac ggtcacgatc cgcaggcggt gaaggaagcg atccttgaag
     4261 cgcaaagcgt gaaagataag ccgtcgctga ttatctgccg tacggtgatt ggctttggtt
     4321 cgccgaataa agcaggtaag gaagaggcgc acggcgcacc actgggggaa gaagaagtgg
     4381 cgctggcacg gcaaaaactg ggctggcacc atccgccatt tgagatccct aaagagattt
     4441 atcacgcctg ggatgcccgt gaaaaaggcg aaaaagcgca gcagagctgg aatgagaagt
     4501 ttgccgccta taaaaaggct catccgcaac tggcagaaga gtttccccga cggatgagcg
     4561 gtggtttacc gaaggactgg gagaaaacga ctcagaaata tatcaatgag ttacaggcaa
     4621 atccggcgaa aatcgctacc cgtaaggctt cgcaaaatac gcttaacgct tacgggccga
     4681 tgctgcctga gttgctcggc ggttcggcgg atctggctcc cagcaacctg accatctgga
     4741 aaggttctgt ttcgctgaag gaagatccgg cgggcaacta cattcactac ggggtgcgtg
     4801 aatttggcat gaccgctatc gccaacggca tcgcgcacca cggcggcttt gtgccgtata
     4861 ccgcgacgtt cctgatgttt gttgaatacg cccgtaacgc cgcgcggatg gcggcactga
     4921 tgaaagcgcg gcagattatg gtttataccc acgactcaat tggcctgggc gaagatggtc
     4981 cgacgcacca ggctgttgag caactggcca gcctgcgctt aacgccaaat ttcagcacct
     5041 ggcgaccgtg cgatcagatg gaagcggcgg tgggctggaa gctggcggtt gagcgccaca
     5101 acggaccgac ggcactgatc ctctcaaggc agaatctggc ccaggtggaa cgtacgccgg
     5161 atcaggttaa agagattgct cgtggcggtt atgtgctgaa agacagcggc ggtaagccag
     5221 atattattct gattgccacc ggttcagaga tggaaattac cctgcaagcg gcagagaaat
     5281 tagcaggaga aggtcgcaat gtacgcgtag tttccctgcc ctcgaccgat attttcgacg
     5341 cccaggatga ggaatatcgg gagtcggtgt tgccttctaa cgttgcggct cgcgtggcgg
     5401 tggaagcagg tattgccgat tactggtaca agtatgttgg tctgaaaggg gcaattgtcg
     5461 ggatgacggg ttacggggaa tctgctccgg cggataagct gttcccgttc tttggcttta
     5521 ccgccgagaa tattgtggca aaagcgcata aggtgctggg agtgaaaggt gcctgatggt
     5581 gatcgccgga tgctgtatgt tcccctcacc ctaaccctct ccccagaagg gcgaggggac
     5641 ggtccgtgcg ctttttaggg ccgcatctgc gattttgttt aacgggtgat ccacaacgtg
     5701 ggcctggcgt ctggcccatg ccagttatcg caggtaggtt ctgcggcgta acgcaccagg
     5761 cgaaaacgct gaccgtcaaa gcgccagcgc gcctgaatgc cacaatcgct taatccgcgc
     5821 cctttcgcta aggtcaccag ttcacgcgat ttctcatcaa atgttgcgtt catcagttcc
     5881 agttcattcg tctcctggcc gttgttgaac ggcaaacgca accgaaccgg gcgcgaggct
     5941 agcggctttt tacgcgacac aatccatgcc aaatcaatgg tgttataggc ccctgcctca
     6001 cagctaatca tcatcagtgc tttatcatcg gtcagcgcag tgacattcac ctcacgacgt
     6061 aacggatcaa gcgagcagcg cagaccattc atccgccagt ttccataatc cagcaaatcg
     6121 ttacgttctt cgagtgagag tggcgtcggc gttgggttaa ccaccgcgac ctctttcagc
     6181 gcaggcgcgg gcggtacgct gagaggcggt tcgtcccctt tcttgatcca cgcggtttca
     6241 ctgccaacgc gcttttgctg agcatcaata aacaacaacg ccgcttttaa gccgctcaga
     6301 gaaatggtct gattgccgtc gcgtaaggtg atcgccttcc cttcctgaat tatctgcaaa
     6361 aacgcggtga tggttgccgt atcatcggtt actaataacc atggtgaaat ccgccacttg
     6421 tcaccactta atgccaacgg ctcaccatct aatagcagcc gtggcgctat ctccccttca
     6481 gacgcatccg gcgacttcaa cccaccgcgc tcaatacgta aaacggcatc ggtatgcgcc
     6541 ccagcgctgc ggctcagggt catcaccagt ccattatgat cgcccgtatt gcgcgccaca
     6601 caaaaatttt gattattaca ggtgacctgc cagtcggaaa acgcccgttg cgctggtgcc
     6661 gcccacacca aagacgtcgg caacaaagcg aaaaaaaaga gaaggaaaat gcgatagcgc
     6721 atggacggta cgaccccaga agttcaaaca aacagcacaa gtcgtatctt cgttgcgcag
     6781 gtagcgcggc tcaatcggat ttgtcggatt gagccaataa taaacaacga aatattattg
     6841 ctatttttca gtccattaaa tgtgacattt gcaaatagtt aagcaataac accgtcttac
     6901 cgtcacgtat ctcgccggtt ttgatcatct ccagcgcctg gctgaacggc agctcgagca
     6961 cttcaatatc ttcatcttca acaccgccac cggcgttagc gcgctgattg tcactgtatt
     7021 cggcgataaa aaagtggatt agctcagtca caccgccagg cgacatatac agttcaaata
     7081 atttgcgcac ttcgcccact tcatagcccg tctcttcaat cgcttcttta cgaatgcaca
     7141 cttccggttc gtcgttatcc agcagcccgg cgcaggtttc aatcagctgc ccgctttcat
     7201 tgccattaac ccaggtagcg acacggaact gacgaatcag aaccacgctc tttttcttcg
     7261 cgttgtacag gaggatcgtc gcaccattac cgcgatcgta aacttcacgt ttatggcgga
     7321 taacttcgcc atctttgcgc gtgagatcgt aagtaatgtt gtgcagggtg aaatagttat
     7381 cggagagaat tttgtcttta atgagggtga tttgttgcgt cataccgact ccacagcgcg
     7441 aaatgaacaa ttatattacg ctgtgaagtc gggtttgtct gcgcaggcta tcagtgagat
     7501 ttgactgatt ttacccccag ccagtcaata atcccctgtg ccgcatgacg tccttctgcc
     7561 attgcggtaa ccaccagatc cgcaccacgc acggcgtcgc caccggcgaa gattttcgga
     7621 ttggtggtct ggtaacggta ctggctttcc acatccgcga tgatgcggcc ccatttgtct
     7681 accgttacac cgtgcgactc cagccacggc atcccgtgtg gattgaagcc aaacgccata
     7741 atcaccgcat cggctggcat gacaaattca ctgccctcaa tcggtacagg tcgccgacgc
     7801 ccctgggcat ccggctcgcc aagacgcgtg cgcaggaaac gaattccgca aacgtgacct
     7861 tgctcattca gctcaagcgc caccggctgg acgttaaatt cgaagttggc cccctcttcg
     7921 cgggcgttct tcacctcttt cttcgagcct ggcatgttag cttcatcacg acgataagcg
     7981 caggtgacgt tactcgcgcc gtggcgcagc gcagtacgca cacagtccat cgcggtgtcg
     8041 ccgccgccca gtaccacgac gttaagtccg gcggtattga taaacggctc ttccggtagc
     8101 tcttcgagcc ccatcacctg tttagtgttg gcaatgagga acggcagcgc gtcataaacg
     8161 cccggcgcat cttcattggg taaacccgct ttcatggaac ggtaagtgcc tacgccaacg
     8221 aagaccgcgt cgtattgttc caaaagcaaa tccaaagaga catctttacc cacttcacaa
     8281 ttgagttcga agtgaatccc catcgcgctg aagatttccc gacggcgtgc cagcagggat
     8341 ttatccagtt tgaaagaagg aatgccgaaa gtgagcaagc caccgatttc tggatggcga
     8401 tcgtacaccg tcaccgccac gccattgcgg gtcagaacat ccgcacaggc cagccctgcc
     8461 ggacctgccc cgataatcgc cacccgcttg tccactttgg tgacatgact taagtcagga
     8521 cgccaacctt tcgccaacgc ctgatctgaa atatagcgtt caatgttgcc gatagttacc
     8581 gcgccgtgct catcgcgaat agtacaggca ccttcacaca aacggtcttg cggacaaacg
     8641 cgtccggtaa tttccggcag ggtgttggtc tggtgagaaa gctcgacggc ggcgtcgatg
     8701 tttccggctt tcaccagttc aatccactgc ggaatatggt tatgcagcgg gcaggtccat
     8761 tcacaaacgc tgtgctcgcc gaacttaagg cagcgcgagg cttcccgttg tgcctggtcg
     8821 gcgcgaaatg gcagataaat ttcatcaaaa ccggttttgc gcgcttcaat cgccagttta
     8881 tccggctcgc cacgcgcggg cgttgcctgc atttgttcga ctttactcat taccggcatt
     8941 tcttgtgcag cggtactggc atgccacggt tgatgctcct gacgtgcggt gcgcaagcgg
     9001 cgggatttcg ccatgccgga gagtgcgacg tcagtgacca gttgcagcgc gtccgccggg
     9061 caattctcga cacaggcagg accgttttcg cgccctgcac aaaggtcgca tttatgcgcc
     9121 gtggctttta cttttcctgc cgcgacgggt gtcaggacga tttgcatcgt accaaacgga
     9181 caggccacca cgcaggattt acagccaata cacttttgct gattgacctg aatgctgtca
     9241 tcaacgtggc tgattgcgcc attagggcag ctacgggcgc agggcgcatc ttcacaatgg
     9301 tgacaggtca ctgcactacg ttgctgctga tgtttgataa ccgtaattcg gggatgaaaa
     9361 tggtgttggc tcaggacatg ttgctcatca ttgtgagcca tgacacaggc gatttcacaa
     9421 gcatgacaac ccagacattg ctgactgttg gccataataa aacgattcat aacgaccttc
     9481 ttttttggtt gtaaaaacct tattctttat atgagtgttg ttattacccg acttacaggg
     9541 gaaccggcaa tgttcatgtg cccagaataa gtaactattt cgctataaac tgtggcagat
     9601 caaataatcc catcactgac taaattgcgt ttcagcgaac tggaacatta atgatttttt
     9661 gtggagaaga cgcgtgtgat tgttaaacga cccgtctcgg ccagtctggc ccgggccttt
     9721 ttttacattg tgctgctgtc gattctttcc acgggtatcg ctctgctaac tctggcgagc
     9781 agtttgcgcg acgctgaggc tatcaatatt gccggatcgc tgcgtatgca gagttaccgc
     9841 ctgggctacg acttgcaaag tggcagtcca caactcaatg cacatcgcca gttgtttcag
     9901 caggcactgc att
//
LOCUS       DAZSNM010000151         9763 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZSNM010000151 DAZSNM010000000
VERSION     DAZSNM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.151"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            383..1501
                     /locus_tag="ACHW42_003052"
     CDS             383..1501
                     /locus_tag="ACHW42_003052"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR8944771.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="ACHW42_003053"
     CDS             1567..1815
                     /locus_tag="ACHW42_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFR8944772.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="ACHW42_003054"
     CDS             1880..2248
                     /locus_tag="ACHW42_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR8944773.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHW42_003055"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHW42_003055"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR8944774.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFAAPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHW42_003056"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHW42_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR8944775.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHW42_003057"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHW42_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR8944776.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHW42_003058"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHW42_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR8944777.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHW42_003059"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHW42_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2388 a         2705 c         2369 g         2301 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccgc gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccagattaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagctg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg cag
//
LOCUS       DAZSNM010000152         9757 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZSNM010000152 DAZSNM010000000
VERSION     DAZSNM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.152"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            38..778
                     /locus_tag="ACHW42_003060"
     CDS             38..778
                     /locus_tag="ACHW42_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR8944778.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            837..1223
                     /locus_tag="ACHW42_003061"
     CDS             837..1223
                     /locus_tag="ACHW42_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR8944779.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1232..1561
                     /locus_tag="ACHW42_003062"
     CDS             1232..1561
                     /locus_tag="ACHW42_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR8944780.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1533..4574
                     /locus_tag="ACHW42_003063"
     CDS             1533..4574
                     /locus_tag="ACHW42_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR8944781.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4574..4903
                     /locus_tag="ACHW42_003064"
     CDS             4574..4903
                     /locus_tag="ACHW42_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8944782.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4903..5601
                     /locus_tag="ACHW42_003065"
     CDS             4903..5601
                     /locus_tag="ACHW42_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR8944783.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5606..6349
                     /locus_tag="ACHW42_003066"
     CDS             5606..6349
                     /locus_tag="ACHW42_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR8944784.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6313..6888
                     /locus_tag="ACHW42_003067"
     CDS             6313..6888
                     /locus_tag="ACHW42_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR8944785.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSDGWYQVRIAG
                     RDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVAIWMPGVGIM
                     ASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            6949..>9757
                     /locus_tag="ACHW42_003068"
     CDS             6949..>9757
                     /locus_tag="ACHW42_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR8944786.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPA"
BASE COUNT         2281 a         2313 c         3176 g         1987 t
ORIGIN      
        1 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
       61 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
      121 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
      181 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
      241 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      301 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      361 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      421 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      481 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      541 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      601 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      661 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      721 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      781 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      841 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      901 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      961 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
     1021 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
     1081 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
     1141 gggttctgtc gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg
     1201 cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     1261 gttccggcgg gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga
     1321 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     1381 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga
     1441 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     1501 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     1561 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     1621 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     1681 agactcaggc ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag
     1741 cccatgcgca gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg
     1801 aacaaacccg tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg
     1861 ccagggcact ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca
     1921 gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac
     1981 aggccagaaa cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca
     2041 cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt
     2101 ttatccgcca gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc
     2161 gcgccagggc ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg
     2221 agcgggcagg aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc
     2281 tgggggtgtt aatcagtcag atggcgcgag gcaattttgg tgcgctgagg ggatccggga
     2341 taacgctggc taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga
     2401 ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg
     2461 acggccggaa ggagggcgag aaatttaacc gtcagctggc gctgacggga cattatgccg
     2521 gtgtcactgt cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc
     2581 aacatgcggc agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg
     2641 atattggtat ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca
     2701 gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc
     2761 tggacaatgc gctgcatttt ttgaatgcca ctcagcttga gcagatacgc gtccttgggg
     2821 agcaggggcg gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg
     2881 gtaaacgcac atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct
     2941 tgtctgactg gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc
     3001 tggatgcgca gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga
     3061 caaacgcctc cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa
     3121 aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac
     3181 agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac
     3241 gacatcagcg ggaaattgcg cgtattaatg ccatgcagta cgccgaccag gctgtcaggg
     3301 atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc cattaagaaa aatacacggg
     3361 caacccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcag caggcacagg
     3421 tggaagggca gattgctgct gccagacagt cagcaggcat tgccactgaa aggatgactg
     3481 aagcgcataa acagcttctg gccctgcagc agcgcatcag cgacctggac gggaaaaagc
     3541 tgacggcaga tgaaaagagt gtgctggccc gtaagaatga gctgattcag gcgctgacgc
     3601 tgctggatgt gaaacagcag gagctgcaga aacagacagc gcttaacgac ctgagaaaaa
     3661 aaacggttca gctgaccagc cagctggcag acaaagaacg tgcactgcgt gagcagcaca
     3721 atctggatat tgccactgca ggtatggggg ataagcagcg gcagcgctac caggcacagt
     3781 tgcgcatccg gcaggaatac cggcaacagt tgcaacagct tgagaatgac agtcgccaga
     3841 aaggcactta cgggacggag gactaccgga gggctgagga ggtgctgaag gggagcctga
     3901 agcgacaact gaatgaaaac aaacgctact ggcaggaact ggaagtggcg cagggcgact
     3961 ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc ggatgcggat aatgcggcgg
     4021 gaacggcaga acagatgttt acagtggcat tcagcagtgc cggtaatgca ctggcgacat
     4081 tctgtaccac cggtaagctg aattttaaat ccttcacctc ttccctgttg tcagatatgg
     4141 ccagaattat ggcacagatg gccatgatgc aggcggtaaa gggcgtcggt tctttattcg
     4201 gcttcacgac taatgctgat ggcggtgttt accagtctgc tgatttgagt cgctacagtg
     4261 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     4321 aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc tgatggtaag ctgggggttg
     4381 tggcggatac tggtggttca ggcatggtga tgtttgcccc gcagtacaac attgagatca
     4441 ataacgacgg cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgacctcg
     4501 ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg tgatggtggc cggttaagtg
     4561 gagcataccg gtaatggaga cgtttcactg gaaagtgcgc ccggatatga atgtggtatc
     4621 agagccgaaa gtggtgacag tgaagctggg cgatggttat gaacagcgtc gtgcggcggg
     4681 actgaataac cagttgtcga cttacagcgt gacgatacgt gttcgtaaag gtgaacaccc
     4741 atctttaaaa gcctttctgg aacggcacgg tggcgtccgc gcatttcagt ggacgccacc
     4801 ttatgactgg aaactgatca gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt
     4861 atgggtgact gtaacggccg attttgaaca ggtggtgaac tgatgcagga tatccggcag
     4921 gaaacactga atgaatgcac ccgtgcggag cagtcagccc gggtggagct ctgggaaatc
     4981 gatctgacag aggtcggtgg tgagcgttat tttttctgta atgagcagaa cgaaaaaggt
     5041 gagccggtca cctggcaggg gcggcagtat caggcatacc ccattcaggg gtcggggttt
     5101 gaactgaacg gcaggggctg tgctgcccgt ccgacactga cggtctctaa cctgcacggc
     5161 atggtcaccg ggatggcgga agatctgcag agtctggtcg gcggaacggt ggtccggcgt
     5221 aaggtttacg cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat
     5281 ccggaacagg aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagcgcggtc
     5341 agtgcctctt ttgtgctggc cacaccaacg gagacggatg gcgcggtttt cccggggcgt
     5401 atcatgctgg cgaatacctg tatgtggact taccgttctg atgagtgtgg ttacacgggc
     5461 agggcagtgg ctgacgagtt cgacaaacca acaacggata tccggaagga caaatgcagc
     5521 aagtgtatgc gcgggtgtga gttgcgcaac aataccggta atttcggcgg tttcctttcc
     5581 atcaataaac tttctcagta aatccatgac acagacagaa tcagcgattc tggcgcacgc
     5641 ccggcgatgt gcgccagcgg agtcgtgtgg cttcgtggtg agaacgccgg agggagacag
     5701 gtatcttccc agcgagaata tctccggtga gccggaggaa cggttccgga tggctccgga
     5761 ggactggctg cgggcacaaa tgcagggtga gattgtggca ctggtccaca gtcatcccgg
     5821 tggtctgccc tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg
     5881 gtggctggtc tgccggggga cgattcacaa gttccgctgt gtgccgcatc tcaccgggcg
     5941 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     6001 ggggattgag atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct
     6061 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc
     6121 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
     6181 cggcgatggt gagctgctgc accatattcc tgaacaactg agtaaacgag agaggtatac
     6241 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggaatggc acgcatctgc
     6301 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct
     6361 gaagccatcc gcgcactggc cacacagctc ccggcgtttc gtcagaaact gagtgacggc
     6421 tggtatcagg tgcgcattgc cgggcgtgat gcaggtgaaa ccgaattgtc tgcccgtctt
     6481 aatgagccgc tggcaaatgg tgcagtgatc cacatagtac cgcgtctggt gggagctaaa
     6541 agtggcggtg tgtttcaggc ggtgctgggg gcagctgtta tggcggttgc tatatggatg
     6601 ccgggggtag gaattatggc gagtaatctg ctgttttctc tcggtgccag tatgacgctt
     6661 ggcggtgttg cacagatgct ggcaccgaaa gccagaactc cccgtacaca gacaacggat
     6721 aacggcaaac agaacacctg tttctcctca ctggataaca tggttgccca gggcaatgtt
     6781 ctgcctgttc tgtacggtga aatgcgcgtg gggtcgcggg tggtatctca ggagatcagc
     6841 acggcagacg aaggggatgg tggtcaggtt gtggtgattg gtcgctgatg caaaatgttt
     6901 catgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat gggtaaaggc
     6961 agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc tgaaatccac gcagttgctg
     7021 agtgtgattg atgccatcag cgaagggccg gttgacggtc cggtggatgg attaaaaagc
     7081 gtgctgctga acggtacgcc ggtcctggat agcgagggga agacaaactt ttccggtgtt
     7141 acggtggtgt tccgcgccgg cgagcaggag cagacaccgc cggaggggtt tgaatcttcc
     7201 ggctcagaga ctgtgctggg tacggaagtg aaatacgaca cgccgatcac ccggaccatc
     7261 acgtcggcaa acattgatcg tctgcgcctg accttcggtg tgcaggcact ggtggaaacc
     7321 acctcaaagg gggaccggaa tccgtcggaa gtccgcctgc tggttcagat acagcgtaac
     7381 ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacttc gcagtatctg
     7441 gcctcggtgg tggtgggtaa cctgccgccg cgcccgttta atatccggat gcgcaggatg
     7501 acaccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc gtacaccgaa
     7561 atcatcgatg tgaaacagtg ctacccgaac acggcgctgg tcggcgtgca ggtggattca
     7621 gagcagttcg gtaaccagca ggtgagtcgc aattatcatc ttcgcgggcg cattctgcag
     7681 gtgccgtcga actataaccc gcagacgcgg caatacagcg gtatctggga cggaacgctt
     7741 aagccagcat acagcaacaa catggcctgg tgtctgtggg acatgctcac tcatccgcgc
     7801 tacggcatgg ggaaacgtct tggtgcggca gatgtggaca aatgggcgct gtatgtcatc
     7861 ggccagtact gcgaccagtc agtgccggac ggatttggcg gcacggagcc gcgcatcacc
     7921 tgtaacgctt acctgaccac acagcgtaag gcgtgggatg ttctcagtga tttctgctcg
     7981 gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca ggaccgaccg
     8041 tcggataaag tgtggaccta taaccgcagt aatgtggtga tgccggatga tggcgcgccg
     8101 ttccgctaca gcttcagcgc cctgaaggac cgccataatg tcgttgaggt gaactggatt
     8161 gatccggaca acggtcatga gacggcgaca gagcttgtgg aggacacgca ggccattgtc
     8221 cgttacggtc gtaacgtcac gaagatggat gcctttggct gtaccagccg gggacaggcg
     8281 caccgcgccg ggctgtggct gattaaaacg gaactgctgg aaacgcagac cgtggacttc
     8341 agcgtcggcg cagaagggct tcgccatgta ccgggtgatg tcattgaaat ctgcgatgat
     8401 gattatgccg gtatcagcac cggtggtcgc gtactggcgg tgaacagcca gacccggacg
     8461 ctgacgctcg accgtgaaat cacgctgccg tcctccggta ccacgctgat aagcctggtt
     8521 gacggaaatg gcaatccggt cagcgtggag gttcagtccg tcaccgacgg cgtgaaggtg
     8581 aaagtgagcc gggttcctga cggcgttgca gaatacagcg tgtgggggct gaagctgccg
     8641 acgctgcgcc agcgcctgtt ccgctgcgtg agtatccgtg agaacgacga cggcacgtat
     8701 gccatcaccg ccgtgcagca tgtgccggaa aaagaggcca tcgtggataa cggggcacac
     8761 tttgacggtg accggcgcgg cacggtgaat ggtgtcacgc cgccagcggt gcagcatctg
     8821 accgccgaag tcaccgcaga cagcggggaa tatcaggtgc tggcgcgatg ggacacgccg
     8881 aaggtggtga agggtgtgag cttcctgctt cgcctgaccg tggcagcgga cgacggcagt
     8941 gagcggctgg tcagcacagc caggacgacg gaaaccacat accgcttcag gcagctggcg
     9001 ctggggaatt acagtctgac agtccgggcg gtaaatgccc gggggcagca gggcgatccg
     9061 gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg tcactattga actgataccg
     9121 gggtattttc agataacggt ggtcccgaaa ctggctgtat atgacccgac ggtgcagttt
     9181 gagttctggt tttcggaaaa gcggattgcg gatatcaggc aggttgaaac cagcgcccgc
     9241 tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc gggccatgat
     9301 tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcatttgt ggaggccgtc
     9361 ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt ataaagggtt gatcaataaa
     9421 acgcatctcg gcaaggagtt gtggacgcag attgataacg gtcagcttgc gccggacctg
     9481 actgaaatca ggacgtccat tacgaatgtc agcaatgaaa tcacgcaaac cgtcaataaa
     9541 aaactggaaa atcagagtgc tgcaatccag cagatacaga aagttcaggt tgatacaaat
     9601 aataacctga acagcatgtg ggctgtgaag ctgcaacaga tgaaggacgg acgcctttat
     9661 attgcgggta tcggtgccgg tattgagaat acgccagcag gaatgcagag tcaggtgctg
     9721 ctggcggcag acaggattgc gatgattaat cctgcga
//
LOCUS       DAZSNM010000153         9686 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZSNM010000153 DAZSNM010000000
VERSION     DAZSNM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9686
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.153"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(44..214)
                     /locus_tag="ACHW42_003069"
                     /pseudo
     CDS             complement(44..214)
                     /locus_tag="ACHW42_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHW42_003070"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHW42_003070"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR8944787.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="ACHW42_003071"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="ACHW42_003071"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR8944788.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="ACHW42_003072"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="ACHW42_003072"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR8944789.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="ACHW42_003073"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="ACHW42_003073"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR8944790.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="ACHW42_003074"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="ACHW42_003074"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR8944791.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6242
                     /locus_tag="ACHW42_003075"
     CDS             5109..6242
                     /locus_tag="ACHW42_003075"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR8944792.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6331..6864
                     /gene="blc"
                     /locus_tag="ACHW42_003076"
     CDS             6331..6864
                     /gene="blc"
                     /locus_tag="ACHW42_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR8944793.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="ACHW42_003077"
     CDS             complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="ACHW42_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR8944794.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="ACHW42_003078"
     CDS             complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="ACHW42_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR8944795.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="ACHW42_003079"
     CDS             complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="ACHW42_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR8944796.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7788..8354)
                     /gene="efp"
                     /locus_tag="ACHW42_003080"
     CDS             complement(7788..8354)
                     /gene="efp"
                     /locus_tag="ACHW42_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR8944797.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8396..9424
                     /gene="epmB"
                     /locus_tag="ACHW42_003081"
     CDS             8396..9424
                     /gene="epmB"
                     /locus_tag="ACHW42_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR8944798.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2481 c         2466 g         2274 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg
     5881 atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa
     5941 ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca
     6001 gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta
     6061 agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     6121 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     6181 aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt
     6241 aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac
     6301 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
     6361 acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta
     6421 gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat
     6481 caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
     6541 ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa
     6601 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
     6661 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
     6721 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
     6781 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
     6841 tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg
     6901 attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg
     6961 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt
     7021 aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata
     7081 acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact
     7141 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaatttgggg
     7201 ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
     7261 attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca
     7321 aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc
     7381 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     7441 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     7501 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     7561 catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     7621 gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta
     7681 cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     7741 gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     7801 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc
     7861 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     7921 tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta
     7981 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat
     8041 ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     8101 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     8161 gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     8221 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     8281 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     8341 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     8401 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     8461 tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     8521 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     8581 catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga
     8641 gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc
     8701 tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     8761 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     8821 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     8881 ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact
     8941 ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc
     9001 ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct
     9061 ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa
     9121 attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga
     9181 taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta
     9241 ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga
     9301 agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact
     9361 ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca
     9421 gtaactgatt aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg
     9481 cctacatcgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt
     9541 tacgccgcat ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct
     9601 ttgcacgttg cataaccttt aattattcat atgcattgct gttgttactt tttatatagc
     9661 taacaccaat aattaacctt tctttt
//
LOCUS       DAZSNM010000154         9622 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZSNM010000154 DAZSNM010000000
VERSION     DAZSNM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9622
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.154"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..141)
                     /locus_tag="ACHW42_003082"
     CDS             complement(<1..141)
                     /locus_tag="ACHW42_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR8944799.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTL"
     gene            complement(155..814)
                     /gene="pgmB"
                     /locus_tag="ACHW42_003083"
     CDS             complement(155..814)
                     /gene="pgmB"
                     /locus_tag="ACHW42_003083"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR8944800.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(811..3078)
                     /gene="ycjT"
                     /locus_tag="ACHW42_003084"
     CDS             complement(811..3078)
                     /gene="ycjT"
                     /locus_tag="ACHW42_003084"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR8944801.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(3075..4129)
                     /locus_tag="ACHW42_003085"
                     /pseudo
     CDS             complement(3075..4129)
                     /locus_tag="ACHW42_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(4139..4914)
                     /locus_tag="ACHW42_003086"
                     /pseudo
     CDS             complement(4139..4914)
                     /locus_tag="ACHW42_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            complement(4932..5984)
                     /locus_tag="ACHW42_003087"
     CDS             complement(4932..5984)
                     /locus_tag="ACHW42_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR8944802.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6015..6857)
                     /locus_tag="ACHW42_003088"
     CDS             complement(6015..6857)
                     /locus_tag="ACHW42_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR8944803.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(6844..7725)
                     /locus_tag="ACHW42_003089"
     CDS             complement(6844..7725)
                     /locus_tag="ACHW42_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR8944804.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(7746..9029)
                     /locus_tag="ACHW42_003090"
                     /pseudo
     CDS             complement(7746..9029)
                     /locus_tag="ACHW42_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(9043..>9622)
                     /gene="ycjM"
                     /locus_tag="ACHW42_003091"
     CDS             complement(9043..>9622)
                     /gene="ycjM"
                     /locus_tag="ACHW42_003091"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFR8944805.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         2285 a         2672 c         2412 g         2253 t
ORIGIN      
        1 cagggtggtc gatttaccgc agcccgacgg gccgacaaat acgatgaact ctttatcggc
       61 aatttccagg ttgaagtcct tcaccacatg cacctggtta tcgtagattt tttgaatatg
      121 ttgtaacgaa agctgagcca tgttgattcc tttgctatac gttttgccag aaggccgata
      181 accgcgacca ggtcagggag tccgttgagg gcaacagtaa ttgcgccccg gttaagcccg
      241 caccgatccc caccgagcgc ataccgctgg cgttaatggc gtcaatgccc gcctgcgcat
      301 cttcaatgcc gatacatgcc tgcggcggca cgcccagccc ggcacaggca gcgagaaaga
      361 tttccggatc cggtttcgag tttttaagtt gggaagcatc cgcgcagaag gtgaaaaact
      421 cacgcagctc cagcgccgct aaaatcgtcg gcgcattcag ggagacggaa gccagcccta
      481 ccggaatctg ctgtgcacgg agctctgcca gcaaattgcg aataccgggc agcacagcat
      541 taaccgtcag ctcgcgcagt gagtggacgt aaagcagatt tttgcgatac gccagttgcg
      601 cccgctcctg cgggttaaag tcgccctctt tgcccccgtg ttgcagaatg cgccgcagag
      661 actcatcgcg gctgatccct tttagagatt cgttaaactg cgcatcaata ctgatgccaa
      721 tttcagcggc aatctgctgc cacgcctgga aatgcagatg cgcggtatcg gtgattacac
      781 catccagatc gaaaattacc ccttgcagtt tcattcatcc tcctgatgcg tggtagcggt
      841 cccattgaag ggcaaaataa aatcacccaa acagaaaaca gattcttcag ccacggatat
      901 aagctgaccg tccaaacgca gtgaaacggg cgcagaagtt cgaatcgcaa tacgctgcgc
      961 gtcgagagtg acctgtaatt cgcagccctg ccagaacaga gggaaagaca actgttgcca
     1021 ctgctcaagt aacgccggat ttagatgcaa ttcaccgtca cgcacgctca ccccggcaaa
     1081 accctgaatc gcccccagcc agatagcgcc agttgcggca gcatggatac catcatcaca
     1141 actatgcgga tcagcaccaa gatcgatttc agtcccctcg cgccagaact gataactttg
     1201 ggtcagcagg ccacagcgtg cagcaacaat gccgtggatt gctttactta atgacgagtc
     1261 gtgaatagtg cgcggttcat aaaattgcag attggcaaga cacgatgccg atgagaactg
     1321 ctctggcagc atgtaattga gcatcaccac atcagcttgt ttgaggatct gcatctcgtt
     1381 cacttctgcg cgtgaataat ccagcagaat ggtttgcttc cccgccgccg ctttgtattt
     1441 cgccagatta atcgccggct tagtcataaa cgaatcatcc tgcggcaaaa caccgtcggg
     1501 ctgaatttct ggcagccgca gttctttaag gaacatttcg gcacgatgaa taaacgcgtc
     1561 gtcactacag ccaaactggc gggcaatact cagcgcctgc tggacgttgt aatacaccat
     1621 atagctggtg aaggcgttat tattgacatg ttcggtatat tcgtctggcc caataacatc
     1681 atgaatttcc agacggtcgt taaccctcac cgcgcggcta atccagaact ttgcagtttc
     1741 cagaagtagc gccatgcctt catgcgcaat gaaactttca tcccccgtgg tctgccagta
     1801 ttgaataacc gcccaggcga tatcggccac cagatgatgt tccgcctgcg ccgaggccac
     1861 tttttgccgc agcccggtgc gaatgttaat ggcggcaaat tccggcgtct cttcttcgcc
     1921 gctgcgcgcg ctttcccacg gaaatagcgc gccctgccag ccgttgcgtc gcgctttctc
     1981 ctgcgcgcct ggcaagttgt gccagcgata acgcagtaaa cttcgggcaa ccgtcggatc
     2041 gctaaacaga tggaacggca gcaaaaatac ttctgtatcc cagaaaacgt ggcctttgta
     2101 gccttcgccg gttaagcctt ttgccgcaat actgctgcgc tcgccgtgag ccggcgtcat
     2161 gatgcgcaga tgataaagcg cataatctaa cgcttgctga tcgtgcgcat cgccgccatt
     2221 taccgtgata cggcgtttct gccaccattg acgccagttt tctgttgatg ctgcaagatg
     2281 ttggtcgtaa ctctgttgcg cgcacatttc aagctggcga agcgacacgc tgccccactc
     2341 gtctaaagca gcttgcctgt catcccgcca gtcgatccac accagttttt gcaacgtcac
     2401 tgtctcgcct gcatgaagct gcgtactggt atgttgcagt aaacggcgct ctttggcggt
     2461 atagcattgc tgcacatcac cgctcacctg gcagcaacaa ctgatggcca catcactgcg
     2521 cccatcctgg gtggtgtaga tcccctgcat caaatgctga ccaaacaccc gcacctgggt
     2581 ttcgtcgaga tgctggcgac cgtggttggt ttgcgtggcg tcaatgcctg ttgaaatcag
     2641 cactgacgcg tcggcgtcca gtggcataat agtgatttcc agcgcaatga gcggcagttg
     2701 gtctgccgaa acaaagcgac ggctggcgat ggtgtaacct gcgccgttgc tggtacgcca
     2761 gacaacgcta cggcgtaatt ctccactggc aaagtcaagt tcacgctgcc aggcttcgcg
     2821 ggataacgag aaaacctcac catttatggc aatctccatc cccaagatat caggcagatt
     2881 caccagttcg ttgatttcac cttttcccgc ccgatgatac agccccgcca gatacatccc
     2941 tcgcgtctgg cgcgtgtaat cttcttcatg gctggcgcga agcccaagat agccgttccc
     3001 ctgcgccatc agcgatgcat acttgttcag ggtatgctgg ctgaaatggg gttctgataa
     3061 cgttactggc ctggtcataa ttccacacgc gtccctgttt ctgcggattg atataacgcc
     3121 gccaccagtt gctggatgat gtacccctgc tcggcgtcgg ctatcatcac gggcttgccc
     3181 tgtacgtgat tgataaaggc ttccatgctg cgcaaatggc ggttgtcgtc tgccatttcc
     3241 cgttgcatca gcgtcattaa ttcaccgttg ttatcggtgt agatatgtgc tggaaacagc
     3301 gtcgcaccgg ctttatcacc acaaaagctg acgttcataa tcaactgttc gcggatgttg
     3361 agtgcaaatg acgtttccag ccacagaatg ccgccgttat gaaattcaat ggtgccaaac
     3421 agcgaatctt cgacgctgta agttgccgga tcccactcgc caaattgacc acagctcttt
     3481 tgcgtgccga tcttttgaaa actatgcgca ttcacgcttt tcaccgccgg aaagcccagc
     3541 acatacatcg cagcatccag catatgaatg ccgatgtcga tcagcaggcc accaccctgc
     3601 agttctttat tggtaaagac accccagccg ggaacgccgc agcgacgcag ggcgcgggcg
     3661 gtggtaacgt aaatctctcc caaaacgccg ttggtcacct gttcacgcag ctgttgcgta
     3721 tcgagcgcaa acgatggtga aagtcgtagg ccagcacctt gcccagtttg cgcgcggtat
     3781 cgcacatttc ccacgcctgt tctgtcgtca tggcgggcgg tttttcgcac atcacatggc
     3841 agcctgcttc cagtgccatc agggtatgtt cgtaatgaaa acggttaggt gagccgacgc
     3901 taaccacatc aggtttcacc gccagcagca tggcctgcgg atcgtcccac acggatgcat
     3961 tcccgtattt ttctgccagc gcctgcgcct gggaaaggtg gctgtcacag acagccacca
     4021 gttccagatc gttgcgggtg cagtagtacg aagcatgaac cttatccgcc acctggcccg
     4081 cgcctattat cgcgacccgc agcggagagc ttgtcattgc acttttcaca aagacctctt
     4141 agcaggtacg caaccaggcc aacgaatcac ggtacgcctg ggcaggatct tctgcccgga
     4201 tacgcccttc atacaccaca taaccctgat agttatcggc gcgcagctgt tcaaacagcg
     4261 cgtggaaatc gagggtgcca ctgcccggct ggtaacgatg gttatcggca atatgcacat
     4321 gaccgagcaa gtcacggttg tcgtgcagcg cctgcgccag gttatcttct tcgatgttca
     4381 tgtgatagaa atcgccgata atctgcacat gtttaagatt gttttcgacg atgtaacgac
     4441 gagcatcggc gagggtgttg atcatatgat cctgatagcg gtttaacggt tcgagataca
     4501 ccacggttcc ggtacgcgcg gcgacctgtt ccagtacgcg cagggaatca ctcaccagtt
     4561 tgcggtcgcc gtccaggcta cgcggcgagg tcatcggcgg taagcggaag gtaaacatgc
     4621 cccacgctgc cggaaccccc ccacttctgc cagcgcctcg agaatgcgtt cgatctgctt
     4681 taagccatta agacgacgct cttcgataaa gtcaccaatc cagccgtcat agccgccgca
     4741 ggcggtggtc actggtaaac cggtttcttt gatcgccgct ttgacttctt cgatgttgtt
     4801 gaccagcagt ttgccgtcaa tctcaaaacc atcgaagccc atctctttga tataacgaaa
     4861 tctctcgcgg atattttccg gaaagaatgc ctgattctgt gtgccgattt tcattttttc
     4921 attcctttga attaaaacgt aacgcccatt ttgatgctct gttccggatg ctgatcgaca
     4981 tactgcatgt agctttccgg gctgttggca aaggtcacta ccgggtcgat taaatcttcg
     5041 caattgagat aaccgttcat cagcagttcc cagcaggttt cttcaatacg cttacggctc
     5101 cagcgcggat aatccgggtt cggttcgctg cacgcgcggg agaaaacaat tttggcgtta
     5161 ttgaaatgcg cttcgcgtcc gaggttaaaa ccttcagcaa atggtttggc aaacgcgaca
     5221 taggagatgg tgccgccgta ggccaggccg cgcagcgccg attgcagcgc gtcggcgtaa
     5281 ccgctggttt cgataatcac atcagccccc tgcttgccgg tcagcgtttt gatctctttg
     5341 cctacgtcag tgccaatggg gttaaggcag aaatcagcac cgtggcgacg ggcaatatca
     5401 cagcgatggg cgataggatc gacgccaatt accaccgatg cgccagcgcg tttcgccagt
     5461 tggatggcaa tttgaccgat cgcgccaagc cctaccacca cgacaaaatc ccctacgcgt
     5521 acgttggcat cgcgcacgcc gctcatggca aactgcgccg ggtcgtaaca gacggcgttt
     5581 ttccaggagc tgccttgcgg cattttgcgc agcttgtagt tattcactgc gttaatgatg
     5641 accgtctcgg agagcgggcc gtagccgcat acgctgtcgc ccaccgcgta gtctgtaacg
     5701 tcgctgccgc actcgataat gtcgccaacc accatgttgc caagctggaa tttgccaaac
     5761 tcaatgccgc gcggcgcatc tgccggacgc ggggtgaaca tctgccactc gccgttaaag
     5821 tcttcatcga taaacgggct ggcggcgcgg aagtcgacca cttccgttcc gtgtttcggt
     5881 gcgccgaaac gcacgcggat cttcacttca ttggctaaaa tcgcccgatc ttcatactca
     5941 accagcgcag caacacgcgg tgctgtggct actaactttt tcatgtaaaa ctccttgtaa
     6001 agaaactggc cgctttagcc cttcacgcca ccggcggtca aaccactctt aataaaacgt
     6061 tccaaaagcg catacataat caccaccggc aatgcggtca ccagcgaggc cgccatcatc
     6121 cgtccccaga tgtaatcagg cgtactgaac agcgcgttca ggcccactgg taaggtgaaa
     6181 ttgctggcgc tggagaggaa aatcgacgca aacagatagt cgttccacgc caccataaag
     6241 cagtagacga acaccgatat cagcccggac atcgccaggg gcacggtgat acggaagatg
     6301 atttgcagac ggttgaggcc atccatcatc gccgcttctt cgatctcatc agggatggtg
     6361 tcgaagtaac ttttcagcat aaacacggcg gtgggcaggg tttgcgtcac catggtaatg
     6421 atcagcgcca tctcggtgtc gtaaatacca agcgcggtga tgattttgaa cagaggcacc
     6481 accagcaaaa tgccagagaa catatacacc gtgtaaaagc tggcgttgat ggtcatccgg
     6541 cctttaaagc gcaggcggga aagcgcgtaa gctccgagaa tgccgagaaa taccgccacc
     6601 acggaggaaa ccaccgacac caccatgctg ttacggaagt agtcgacaaa cgggaaaatc
     6661 atcgggttaa aaatgtcgac gtaatgctcc agcgtccact gttgcggcag cagcgtagga
     6721 tgcagcgata tcgcctcttt cgcgcttttg aacgaggtca tcagcatcac aaaaaacgga
     6781 aacagcgtga tgatgagaaa cagcgccagc ccgcagtaaa aaccgatacg actgagcgtg
     6841 cgtttatttg ttgccattga ggttcacccg tttcctggtc agcagaatga cagcgaaaat
     6901 aatgatgaag agcaccaccg agatcgccgc cgctttccct aagtcattaa aggcaaaggc
     6961 ggttttgtag agatagacac caagaatatc gaccttggtt gtcagcagat aaacatcagc
     7021 gaacatgtag aacatccaga tggtgcgcag ggtcaccacc gtcgccagga ccggcataat
     7081 tgcgggcagc gtgacgatgc gaaaccgctg ccaggcatta gcgccatcca tctctgccgc
     7141 ttcgtacagc gatttgtcga tggtctgcaa aatcgccaga aacgagataa aggcatacgg
     7201 gaagtagcgc cagatggcga acagcaccac cagcacaaag ctactgcccg gattatcaaa
     7261 ccacagcggt gcctgctcat agagatgcag aagatcgacg ccgaggtagt tcacaatgcc
     7321 gtagccgttg ttgaacatgt atttccaggc aaacaccagc gaaatggacg gcgttacgta
     7381 ggagaggatc accagcgaac gcgcggtttt gcgcaggcgg aactcgcggt tgaaaaacat
     7441 cgccacggcc agcccgagca cggtgctgcc cgccaccacc agcgccgtat accagaccgt
     7501 catccacagc gagtgccaga agccgggatc ggagagaatg cgcacatagt tactcacccc
     7561 aacaaacgtt gactcgatgt tgggattgag cggcagacgt aaaaaactga tttcgatatt
     7621 cgacaccatc ggccacgcca ccagaccgcc cagcagtaat aagctgggcg cgagaagcag
     7681 cagcgcaaac ggcatatcgg aacgacctga aaaaagcctg ttcattgcgg attcctgtcc
     7741 tgggtttagt gctgttcgat cagttcatcc agctttttct ggctcgcttg cagcgtgcca
     7801 gggagatcgg ctttacccac ggtgacgtta tgcaccatgg agctcaccac accagaaccc
     7861 gtcacatcgc ccatgcgggt aaagttttta tcccctactg cgccaaagac ctgaatattt
     7921 ggcagttcac cgattagctg attcggtagt tcacccagcg ccttaataac gtcgttgtct
     7981 ttccaggtgg cggtagtgac aaccgcttta ttcaccggca gcgcagcacc tggcgacatc
     8041 atcacccagt cggcaatgtt gtccgcctgc tccataaagg tgacaaattt ttctgctgct
     8101 tcggtctttt gtccggcggt aatggtcagc gaggtcaaca tgccgtagac cgcagagttt
     8161 ttctcggttg gcaccacgaa accgacgttt ttcgggtcgc cttctttaat cacagccgga
     8221 aggatatagg tggagtaaat cgccatcggc gcggtgccgt tcataaaggc gtctttcact
     8281 tccatgatgt cgttagaacc cggcatggtg ttggcagcaa ggtcgcggta ataggtcagc
     8341 gcctgcatca tctctagtgt atcaagggtg attttgcctt cggcgttaaa gacgttggcc
     8401 tagttggata acgcaaactg ggagaaggat tgttccgtca gcacgctttc tgccgtaggc
     8461 agcgcaatac cgtatttttt attcgccggg tcattcagtt tctgtgcaac gtccagcagc
     8521 tgttgccagt ttttcggctc ctccagcccc gcttttgcca gcacatcttt gcgataccag
     8581 atgccgccaa tccaggcgct gacaggaaca ccggtccatg cgctaccatc ttcggtacgc
     8641 acaatacgca gtacgccatc gtaaaacgcg ccttcaccaa cgttgctgat gaccgtggca
     8701 accgctttgc gatcgataag ctgctcttta tccataactt tggcgtagtc atggctggtt
     8761 tcgatcactt ccggcagcga gccgctacgt gaaagggtga tgactttggt gttatgagcg
     8821 tcttcttcca cgggcacttg tttaacggtg atacctgggt tttctttttc aaaacgggca
     8881 atcaatttgc tgataactgc ctgacgctcc tgctccaccg aagaatgcat aaattcgatg
     8941 gatacatggg ttttattttc ttctttacag cctgaaatca gggcgcagga aaccagtgct
     9001 gataacagca cgatttttga tttaatcatg agatgttcct ttttatttaa tccacataac
     9061 ctgccagggg cgcagcgtta tttcattacc caatatatca acttcactaa tcagatcccg
     9121 accatgcaga tcagtaatat ttacatgctg aatattttta ctgacattaa acaatccagt
     9181 cagacaatta ccatcagcgt tacttctttg aatacgcatt acggatgaat taaccgtatc
     9241 gatggtaaaa ttattatccg gatgaaactc gttatggctg cgacgaagtg taattaaacg
     9301 cgacaactca tgatataccg catgccttaa tgtagcttca tcgttcagtt ctcgtgttat
     9361 ctcttcactg tgatattttt tacggttaat cgcacggtta tatccgagtt tttcgacacc
     9421 tgcgtaatca ttacgcgagc caagaatact ttgaatatat atcgcgggta caccggggaa
     9481 acttaacaaa atcgcatggg caaggataaa cctggtgcaa cgttcttcat cgctactctc
     9541 gcggcggctt aacgcattca tataggtgac attcatctca tagggactgc gcgtgccgtc
     9601 gggattattt ttccagttta ct
//
LOCUS       DAZSNM010000155         9575 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZSNM010000155 DAZSNM010000000
VERSION     DAZSNM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9575
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.155"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..719)
                     /gene="cfa"
                     /locus_tag="ACHW42_003092"
     CDS             complement(<1..719)
                     /gene="cfa"
                     /locus_tag="ACHW42_003092"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR8944806.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFE"
     gene            complement(1010..2221)
                     /gene="punC"
                     /locus_tag="ACHW42_003093"
     CDS             complement(1010..2221)
                     /gene="punC"
                     /locus_tag="ACHW42_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR8944807.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            2334..3266
                     /gene="punR"
                     /locus_tag="ACHW42_003094"
     CDS             2334..3266
                     /gene="punR"
                     /locus_tag="ACHW42_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR8944808.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            complement(3263..4288)
                     /gene="purR"
                     /locus_tag="ACHW42_003095"
     CDS             complement(3263..4288)
                     /gene="purR"
                     /locus_tag="ACHW42_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR8944809.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            4587..4676
                     /gene="cydH"
                     /locus_tag="ACHW42_003096"
                     /gene_synonym="ynhF"
     CDS             4587..4676
                     /gene="cydH"
                     /locus_tag="ACHW42_003096"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR8944810.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            4842..6011
                     /locus_tag="ACHW42_003097"
     CDS             4842..6011
                     /locus_tag="ACHW42_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8944811.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(6157..6738)
                     /gene="sodB"
                     /locus_tag="ACHW42_003098"
     CDS             complement(6157..6738)
                     /gene="sodB"
                     /locus_tag="ACHW42_003098"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR8944812.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(6866..7693)
                     /gene="mepH"
                     /locus_tag="ACHW42_003099"
     CDS             complement(6866..7693)
                     /gene="mepH"
                     /locus_tag="ACHW42_003099"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR8944813.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            8028..8375
                     /gene="grxD"
                     /locus_tag="ACHW42_003100"
     CDS             8028..8375
                     /gene="grxD"
                     /locus_tag="ACHW42_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR8944814.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(8489..8899)
                     /gene="tnpA"
                     /locus_tag="ACHW42_003101"
                     /pseudo
     CDS             complement(8489..8899)
                     /gene="tnpA"
                     /locus_tag="ACHW42_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            8922..>9575
                     /locus_tag="ACHW42_003102"
     CDS             8922..>9575
                     /locus_tag="ACHW42_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR8944815.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     "
BASE COUNT         2391 a         2303 c         2471 g         2410 t
ORIGIN      
        1 tgctcgaaca tccccacaga aacaatacga tcaaactggt cgttcaggtc acgataatct
       61 tgcagcaaaa tggtgacatc caggccttta cagcgttcct gagccatttt ttgctgttcg
      121 gcagaaatgg tgacgccgac cacgcttacg tcatagttag atgccatgta gtgcgctagt
      181 ccgccccagc cgcagccaat atccagtacg cgcatccctg gttttaactg caatttttca
      241 caaatcattt tgagcttcgc ctgctgggca gattccagat tatcggcatc tttccagtaa
      301 gcgcaggaat attgcatgaa gggatcaagc atgcggctga acaagtcatt acccaaatcg
      361 taatgctcct tgccgactat ccaggcacgt tttttactct gcagattgaa gagacgagcg
      421 ccggcaatac gcagcgtgtc tttgaaatga tgggggagtt ggttctcgag acctgcgcgt
      481 aagactttgc taaaaaacat atccagtcgg tcacattccc accagccatc catataactt
      541 tcgcctaacc ccaaagagcc ttcctgcaga acgcgtttaa aaaaatcggg gtttttcaca
      601 cgaatatccg ccggggcaga accgttaatg gctataccgg cacggctaag taattcgttg
      661 gcgatacggt accagttgtc atccggtaca ctgacttctt ctatacacga tgaactcata
      721 gtttctccat cactggtcgg tgatcagaac cttaaaacag cgtagacgct ttttgggctt
      781 tgtgagaaat ctcgcagaaa aaaccgcgag tctataatcc gacgtagaac agagggaaag
      841 gaggaaccct tgccgaaatg accatccata gtgagtcggg aacgatcctg tccccgtaaa
      901 aacataatat ttatcgtagc tttaacaact cgaaattagt ataggccgct aatttcaatc
      961 atacaacaaa attttgttag cagcctaagt ataagtatat cgatatagat cagtgtgatt
     1021 cgctatgagc gacttcggca ttgccatgat tcgggcagtc aacttcttca caacgttgca
     1081 tcatgtaacc cagcgcgacc agcactactg ttgataacat cacgctggtg gtggtgagca
     1141 atggcgtgct gatactaata agccaggaaa ctaccagact tgcgaggaag cacagaccca
     1201 gttgcagagt gttctgcaac gctgcggcgc gaccggttgc atgtgggaag ggacgcagcg
     1261 cctgggcgac aacaataggg tagatcgcgc cattggcaat cgccatcaca cagaatggga
     1321 tcaggatttc gaccagcgac acatggctaa tgaagcccgc agcccaggtc gcaatgacgc
     1381 tgacagcaaa cagcaccagc aaccacggta ataactgctt gccttgccat ttctgcagtg
     1441 cggcgcgaca gccataacca ccaatcagaa acgcgatagt ttgcgggaca taacttaaac
     1501 caataactgc cgggctgtag cccatttcac taaggatgaa cggtgaaccg gtcagccatg
     1561 caaaaaaact ggctgaacag gctgcgtata tcagcacgtt gccgcgatag gttttagaac
     1621 gtagcaggtc ggtaaaggtc agaccatcct gactattgtt acgggccttc gtcgtgggtt
     1681 tgagccagaa aataggcaga atcagcacca cggtaatggc aaacagggtg gcgaaaatcg
     1741 cctgccagga aaaatggacc agcagccagc ttcctaacag gggagccagt gccggagaca
     1801 gacccaccag cggcatgatg gtcgcaaaaa tacggttgac tttctgtgaa ggataataat
     1861 ctgtcactaa tgcttgccag ataaccgccg cggcgcagac ccccacagcc tgtacaaaac
     1921 gcaataccag cagcgtggcg gcgttttcta cccacagcat ccccagacta cctaacgcaa
     1981 aaattgtcag gccgattaat aacaccggtt tacgaccata acggtcggag agtggccccc
     2041 acagaagctg ggctgcggca aaaccggcaa ggaacagact aaggctggca ctgacagcag
     2101 acgcaggcgt ttgcaggtcg gcctgtatgg cggcgaaagc aggcagatac atatcggttg
     2161 ccagaaaacc gagtacgctc aaacccgcca gccagactaa aaatcttttc ccaggttgca
     2221 tgtatattct ctaaatctct caattaaaca ccgccgcaaa gtgtagggag tgaaattagt
     2281 gttgtgaaac ggtaatattt gatgactggt ttcaaaaatt ttgcaggcag actatgtggt
     2341 cagaatattc actcgaagtt gttgatgcgg tagcgcgtaa tggtagtttt agcgctgcgg
     2401 cacaggagct gcatcgtgtt ccttctgcgg tcagctatac cgtgcgtcag ctggaagagt
     2461 ggctggcggt accgctcttt gaacgacggc accgtgatgt ggagctgacc gctgctggag
     2521 cgtggtttct caaagaaggg cgctctgttg tcaaaaaaat gcagatcacc cgccagcaat
     2581 gtcagcagat agcgaacggc tggcgcggtc agttagctat cgcagtggat aatattgtca
     2641 ggccagaacg tacacggcag atgatcgttg atttttatcg ccattttgat gatgtcgaac
     2701 ttcttgtctt tcaggaagtg ttcaacggtg tctgggatgc gctttccgac gggcgcgtgg
     2761 aactggcaat tggcgcaaca cgcgcgattc cggtaggcgg tcgttatgcc ttccgggata
     2821 tggggatgct aagctggagt tgcgttgttg ccagtcacca cccgctggcg ttgatggatg
     2881 gcccgttcag cgatgatacg ttgcgcaact ggccgtcgtt ggtgcgcgaa gacacctcgc
     2941 gaacgctacc taaacgtatt acctggttgc tggataacca aaaaagactt gtcgtacctg
     3001 actgggaatc atcggcaacc tgtatctcgg caggattatg catagggatg gtgccaacac
     3061 atttcgccaa accgtggctt aatgagggga agtgggtagc acttgaactg gaaaacccct
     3121 tcccggattc ggcatgttgt ctgacatggc agcaaaatga tatgtcgcct gcgttaacct
     3181 ggctgcttga gtatctcagc gatagtgaga cgctgaataa ggagtggctg cgggagccgg
     3241 aagagactcc cgcaacgggt gattaacgac gatagtcgcg gaacgggccg tcagccacgg
     3301 agcggcgttc aatcaaacgc ggatgcactt caatggactg cggttcttca cgtttgttga
     3361 cgatacgatc caacagcatg ttgaacgctg tttcacccag cgaatctttt ggctgatgga
     3421 tcgtggtcag cgccggcgta aaatagcgcg cgttgcgcac gttatcataa ccgatcagcg
     3481 aaacatcctg cgggacgcgc aggcccattt catcagcagc acaaagtgcg cccattgcca
     3541 tgatatcgcc accacagaag acggcagtag ggcgatgcgg ctgcgacagg atttgctgca
     3601 tggcgcgata accggattca ggttcaaagt caccctgcac aatccagctt tccggcacct
     3661 tgatcatcgc ttcttccatc gccttcataa aaccggcaag gcggcctgcg ccggtgttac
     3721 gttccagcgg gccggggatg acgccgattt cgcggtgacc gcgttcaatc agataacgcc
     3781 cggccatgta gccgccttcg aacgcgttat caatgaccgc atcggtgaag tcagcttttg
     3841 cttcacccca gtccatcacc accattggga tatggcgata ctcttccagc atcgccagca
     3901 acggctctgg gtactcagaa cacatcacca gcagaccatc gacgcgtttt tgcgccatca
     3961 tcgacagata agcccgctgt ttctcaagat tgttccacgc attacccaga atcagggtgt
     4021 aacctttctg gaagcaattt ttttcaactg cttcaatgat ctcggcaaaa taggccgctt
     4081 cgctgctggt cgccagcaaa ccgatagact tggtgtggtt aaccttcagg ctacgcgcca
     4141 ccgcgctagg ggagtagtgt aattctttaa tcgctgccca cacggcgttg cgcgtttctt
     4201 cagcgacgaa acgtgttttg ttgatcacgt gtgacacagt tgtagtggaa acgtttgctc
     4261 gtttcgctac atcttttatt gttgccattc catttcactc cagaccctaa cttcagctcc
     4321 tgcaaaatcg caaggtaaac gtttgccttt acacacctta cgcacaacaa aagtgttgcg
     4381 gtacgccgga aaaacgacac ggagcgtcag gaaggggtca atggccagta cgcaaatagt
     4441 gtaagcgtgg aattttggcc gatcttgacg aaaaggggaa gtggtaaaaa cacttatcat
     4501 tataaatgac gggagatttt ttcatcacag tgtgtaaaaa tgcgtaatat cactttttgt
     4561 gtggtaataa aaaaggagaa aacttgatga gcaccgacct taaattttca ctggtaacaa
     4621 cggttatcgt cctcggtttg atcgtagccg tgggtttgac tgccgcgctg cactgatttc
     4681 ctctcgaggg agcgcgttct ccctcgcatc tccttcctgt ttgttagcaa aatggcaaaa
     4741 gtgttttctc tttttgttat tttcattttt ttgtgattgc tgtcatgctt tcgccacttt
     4801 aatgaatatc gcgtaatgac gcgaagaggt ggagataagg catgaaaatt aactatccgt
     4861 tgctggcgct ggcgattggc gcgtttggta tcgggacaac ggagttctcg ccaatgggct
     4921 tgttgcccgt cattgcgcgc ggtgtggatg tctcgattcc cgctgccgga atgttaatca
     4981 gtgcctatgc agttggcgta atggtgggcg cgccgctgat gacgcttcta ctttctcatc
     5041 gtgcccgccg cagtgcgttg attttcctga tggcaatttt cacgctaggc aacgttcttt
     5101 ccgccatcgc gccggattat atgaccctga tgctttcacg tattttgacc agcctgaatc
     5161 acggagcatt ttttggtttg ggttcagtcg tggccgcaag cgtggtgcca aaacataaac
     5221 aggccagcgc agttgccact atgtttatgg ggttaaccct ggcaaatatc ggtggcgtgc
     5281 cggcggcgac ctggttgggt gaaaccatcg gctggcggat gtcatttctg gcaacggcgg
     5341 ggctgggagt gatttcaatg gtaagtctgt tcttctcatt acctaaaggt ggtacagggg
     5401 cacgacctga agtgaaaaaa gagctggcgg tattaatgcg tccgcaggtg ctgtctgcat
     5461 tgctgacgac ggtactggga gctggtgcaa tgtttaccct ctacacctat atctctccgg
     5521 tactgcaaag tattacccac gcaacaccgg tgttcgtcac ggcaatgctg gtgctgattg
     5581 gtgtcggatt ctctatcggt aactatctcg gcggcaaact ggcagatcgt tcagttaacg
     5641 gcacgttgaa aggctttttg ttgctgctga tggtgattat gctggcaatc ccgttcctgg
     5701 cccgcaatga gttcggcgca gctattagca tggtggtgtg gggcgcagca acctttgcgg
     5761 ttgtacctcc gttacagatg cgcgtgatgc atgtcgccag tgaagcgcca ggtctgtctt
     5821 catcagtcaa tattggtgcc tttaatcttg gaaatgcgct gggagcagct gctggtggtg
     5881 cggtaatttc cgctgggctg ggatacagct ttgtgccggt gacgggggcg attgtcgcgg
     5941 gactggcatt attgctggtg tttatgtcag ccagaaaaca acctgaaaca gtttgcgttg
     6001 ctaacagcta attaaacaaa aacgcagaag gataaccttc tgcgttacag ttgctgacaa
     6061 cgtgcgattt atcttgccga atgcgctgtt aacgcctcat ccgcgttggc atggtaagcg
     6121 aagcgcatca ggcaatgctg catttgccat cagttattat gcagcgagat ttttcgctac
     6181 gaattcccag ttcaccagcg cccagaagtg ctccagatag ccaggacgtg cattgcgata
     6241 gtcgatgtaa taagcgtgtt cccagacatc aacggtcagc agcggagtcg catcggtggt
     6301 cagcggagta cccgcgttag aggttgaaac gatagccagt ttgccatcgc tgtttttcac
     6361 cagccaggtc cagccagaac caaagttttt gatcgctgca tcagtaaact gcgctttgaa
     6421 atcggcaaag ctgccaaaag atgcggcgat agcttcagcg acttttccag tcggttcgcc
     6481 accggcgttc ggtgccaggc agttccagta gaaagtatgg ttccagacct gagctgcgtt
     6541 gttgaatacg ccaccttcag agctgcgaat aatctcttcc agtgatttac cttcaaacgc
     6601 ggtaccttta atcaggttgt tcaggttagt gacataagtc tgatgatgct tgccgtagtg
     6661 atactcgata gtttccgcag aaatgtgcgg tgccagagca tctttagcat atggtagtgc
     6721 aggtaattcg aatgacattg ctactctcct ttattattaa tttgcatacg tacaatagcc
     6781 ttattgtgcg tatggatagg gtagcaaatt aaaaggtgag acaaaagatg aacttaccct
     6841 gttgccgtaa caacagggta aagttttagc gaagtgtttt tggggtcatt acccgacgag
     6901 cgccaacata gtggcgctgc cagtagtctt cactgagaga agtgatttga atttcctgac
     6961 ctgtgcgcgg tgactgaata aatttgccgt tgccgacata cacgccgaca tgatcggctg
     7021 taccgcgtcc ctgagtacgg aaaaagacca ggtcgccgtt tttcagctca ctacgttcga
     7081 taggtcctgc atcacgcagg tgatacattt cattcgccgt acgcggaata cgaattttca
     7141 ccaaatcttt ataagcgtaa taaaccaggc cgctgcaatc aaaaccggta cgcggtgagc
     7201 tgccgcccca acgatatggc ttaccaattt gctgcatcag tttattcatt gccactttcg
     7261 tggctttttg caccttcgct ttgtgcgcgt cggcaagggt tcctgacgcg gcatttttga
     7321 ctttcacaca atgcgattta tagcccttac gtttggtaca cttttcggta aagctggctg
     7381 aggccgtttt attgacggcg tgtttgctgc gagttttggc gtttttagaa gcggtaatgg
     7441 aagatttttt aacagaagaa gcggttttgc ttttggtcat acttttactg gcggcttttt
     7501 tcgctgtttt ctggctggct ttctgggtct ttttggtggt tgccgtgctt ttctttttat
     7561 ctgctttttt ggtgatatga gtggtagcag aactctccct ggcttgcttt gaagcatggg
     7621 ccataggcgt taaaggcagg gtggtaaaaa gtaaagcaca gagcgtgatc gaaatacggt
     7681 ttatccgcgc cactgagcgt ccctctgtta aggcaggtct atcatgcctg cgtctgattt
     7741 acaaaacttg atgattctaa tgcatagaag aagagaaagt taatagcttt tattgttaaa
     7801 aaatacgttt cgttggcgag cgcaaaaaaa tgcagcagtg aaagctaaaa atggttataa
     7861 atacgccagt ttgccgcgca tcagagaaga aacgaatatt tgaaatgcaa caaatagagc
     7921 aaagggataa aatagcaaaa gcgtgatgaa aatgttattt tccgatgccg cactgacccg
     7981 ctacaatgtc agattactgc cgtaaagaag tttgaggaag caagacaatg agcaccacta
     8041 tcgaaaaaat ccaacgccag attgctgaaa acccgatcct gctgtacatg aaaggttcac
     8101 cgaaactgcc gagctgcggt ttctctgccc aggcagtcca ggcgcttgcc gcatgtggcg
     8161 aacgttttgc ctatgttgat attctgcaga atccggacat tcgtgcggaa ctgccgaaat
     8221 atgctaactg gccgaccttc ccgcaactgt gggttgacgg cgagctggtc ggcggttgtg
     8281 atatcgtgat cgaaatgtat cagcgtggcg aactgcagca gctgatcaaa gaaactgccg
     8341 ctaaatacaa gtctgaagag ccggacgcgg aataatccgt cctgcaatac tgacggatgc
     8401 gaacgggcat ccgtcagtcc tttatccggt gcgccgtaaa acctcgtcct tcagggcggg
     8461 gatataaggc gcggttttcc acctgaccag gtgtttgctg ttgttcaatg tactggcgga
     8521 tgacggatat tggcgcacct tcgcagctac tggcaaaata gccaggactc cacaaaacgc
     8581 ctttgtagta atacctgact gcaatatctg gtcgatctcg tcgcagtaac ctattcgata
     8641 tgcctttgag gctgtttacc agactggata tcgccagttt gggaggatag ttaattaaca
     8701 aatggacgtg atctggttcg ccatccattt caaccagttc agctgcaaaa tcagcacata
     8761 catttgaaaa gtaagtgcgt agtttttctg tcgtgtcatg gtcaaaaatc tggcgtcggt
     8821 atttggtgac aaagaccagg tgaacatgca tcaggaaaac acaatgcctt ccacgccgga
     8881 tatcggtttc ttttttcaca gaccaaagta tcatttgacc tgtgaaacga ttacaggcat
     8941 ttaaattcca gttaagaccc ggtggtcaac aggagcgtga aatgaggcgc ttcgcaggcg
     9001 catgtcgttt cgttttcaat cgtgctctgg cgcttcagaa tgagaatcat gaggccggta
     9061 ataaatacat cccttacggg aaaatggctt cctggctggt tgagtggaaa aacgccactg
     9121 aaacgcaatg gcttaaagat tcgccctcac agccattgca acagtcactg aaagaccttg
     9181 agcgggctta caaaaacttc ttccagaagc gggctgcttt tccccgattc aaaaagcggg
     9241 gacagaatgc tgcattccgc tacccgcagg acgttaagct cgatcaggaa aacagccgta
     9301 tttttctgcc gaaacttggc tggatgcgct accggaatag ccgtcaggtc acgggagttg
     9361 tgaaaaatgt cactgtcagc cagtcctgcg gtacgtggta catcagtatt cagacagaaa
     9421 gtgaagtatc aactccggtt cacccttcag catcaatgat cgggctggat gctggcgtgg
     9481 ctaaactcgc cacgctgtca gatggcacag tctttgagcc tgtaaacagt tttcaaaaaa
     9541 accagaagac gctggcgaga cttcagcgac agtta
//
LOCUS       DAZSNM010000156         9529 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZSNM010000156 DAZSNM010000000
VERSION     DAZSNM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9529
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.156"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1095)
                     /gene="groL"
                     /locus_tag="ACHW42_003103"
     CDS             complement(<1..1095)
                     /gene="groL"
                     /locus_tag="ACHW42_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR8944816.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKL"
     gene            complement(1139..1432)
                     /locus_tag="ACHW42_003104"
     CDS             complement(1139..1432)
                     /locus_tag="ACHW42_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR8944817.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            1708..2964
                     /gene="yjeH"
                     /locus_tag="ACHW42_003105"
     CDS             1708..2964
                     /gene="yjeH"
                     /locus_tag="ACHW42_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR8944818.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(2980..3456)
                     /locus_tag="ACHW42_003106"
     CDS             complement(2980..3456)
                     /locus_tag="ACHW42_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR8944819.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            3793..5229
                     /gene="aspA"
                     /locus_tag="ACHW42_003107"
     CDS             3793..5229
                     /gene="aspA"
                     /locus_tag="ACHW42_003107"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR8944820.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            5347..6648
                     /gene="dcuA"
                     /locus_tag="ACHW42_003108"
     CDS             5347..6648
                     /gene="dcuA"
                     /locus_tag="ACHW42_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR8944821.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            6764..7102
                     /gene="cutA"
                     /locus_tag="ACHW42_003109"
     CDS             6764..7102
                     /gene="cutA"
                     /locus_tag="ACHW42_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR8944822.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            7078..8775
                     /gene="dsbD"
                     /locus_tag="ACHW42_003110"
     CDS             7078..8775
                     /gene="dsbD"
                     /locus_tag="ACHW42_003110"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR8944823.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWLIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            8812..9387
                     /locus_tag="ACHW42_003111"
     CDS             8812..9387
                     /locus_tag="ACHW42_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8944824.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2246 a         2449 c         2386 g         2448 t
ORIGIN      
        1 cagtttttca cggtcgtagt cagaagttgc ttcttcaatc tgctgacgga tctgagcaac
       61 acggccctgg attgcagctt cttcacccac gccatcgatg atagtggtgg tgtctttgtt
      121 gatcacaaca cgtttagcct gacccaggtc ttccagggtt gctttttcca gctccatacc
      181 gatctcttca gagattacgg taccgccagt cagggttgcg atatcctgca gcatagcttt
      241 acgacgatcg ccgaagcccg gagctttaac cgcagcaact ttcacgatgc cacgcatggt
      301 gttaacaacc agagttgcta gcgcttcgcc ttcaacatct tcagcgatga tcagcagcgg
      361 tttgcctgct ttggcaacag cttccagaac cggcagcatt tcgcggatgt tggagatttt
      421 cttgtcagcc agcaggatga acgggctttc cagttctact gcgccagttt ccggcttgtt
      481 gatgaagtaa ggagacaggt agccacggtc gaactgcata ccttcaacca cgtccagttc
      541 gtcctgcaga ccggtaccgt cttcaacggt gataacgcct tctttaccga ctttgtccat
      601 cgcttcagcg atcagtttac ctacggtttc gtcggagtta gcggagatgg taccaacctg
      661 agcaatcgct ttagaatcag agcacggtac ggacagcgct ttcagttctt caactgcagc
      721 ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg ttcatgcccg cagcaacagc
      781 tttcagacct tcagtgatga tagcctgagc cagtacggtt gcagtggtgg taccgtcgcc
      841 tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc tgcgcaccca tattttcgaa
      901 cttgtcttcc agttcgattt cacgagcaac ggaaacacca tctttggtga tggtcggtgc
      961 accgaaagat ttatccagaa ctacgttacg gccttttgga ccgagggtaa ctttcactgc
     1021 atctgccagt acgtttacgc cgcgcagcat tttcacacga gcgtcgttac cgaattttac
     1081 gtctttagct gccattatct ttattcctta aattcgtatg ttcagtgtcg tgcgcggatt
     1141 acgcttcaac aattgccaga atgtcgcttt cggacatgat caacacttct tcattgtcga
     1201 tcttctcgga tttcacaccg tagccatcgt tgaaaataac gatgtcgcca actttcacgt
     1261 ccagcggctt cacttcgcca ttttcaagga tacggccatt gccgacagcc agcacttcgc
     1321 cgcgggtgga tttagccgct gcagagccgg tcagaacgat gccgccagca gatttagttt
     1381 caacttcttt acgcttgacg atcacgcgat catgcaatgg acgaatattc attgataact
     1441 ctcctttgag aaagtccgta tctgttatgg gtgacgccgg agcttacgtg gtttcccggc
     1501 tggtgaccag agaaatgggg atgaggcttc gccccttcaa gggggaaaaa gaaaaaaaat
     1561 tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct cacatttcat cgcaatttct
     1621 tcatcgccgt cataagcgaa tctgattgtg ctaccatcga aaatctacgc agttgcccaa
     1681 aatttgggcg caatcggata tcaacccatg agtggactca aacaagaact ggggctggcc
     1741 cagggcattg gcctgctatc gacgtcatta ttaggcactg gcgtgtttgc cgttcctgcg
     1801 ttagctgcac tggtagcagg caataacagc ctgtgggcgt ggcccgtttt gattatctta
     1861 gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc actatcccag cgcaggcggc
     1921 gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg agcgagtcac cggctggctg
     1981 tttttatcgg tcattcccgt gggtttgcct gccgcactac aaattgccgc cggattcggc
     2041 caagcaatgt ttggctggca tagctggcaa ctgttgttgg cagaactcgg tacgctggcg
     2101 ttggtgtggt atatcggtac tctcggtgcc agttccagtg ctaatctaca aaccgttatt
     2161 gccggactta tcgtcgcact gattgtcgct atctggtggg cgggcgatat caaacctgcg
     2221 agtatcccct tccccgcgcc aggaaatatc gaacttaccg ggttatttgc tgcgttatca
     2281 gtgatgttct ggtgttttgt cggtctggaa gcatttgccc atcttgcctc ggaatttaaa
     2341 aatccagagc gtgattttcc tcgtgctttg atgattggtc tgctgctggc aggattagtc
     2401 tactggggct gtacggtagt cgtcttacac ttcgacgcct atggtgaaca aatggcggcg
     2461 gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg taggagcgtt atggattgcc
     2521 tgcgtaattg gctatctggc ctgctttgcc agtcttaaca tttatataca gagcttcgcc
     2581 cgcctggtct ggtcgcaggc gcaacataat cctgaccact acctggcacg cctctcttct
     2641 cgccatatcc cgaataatgc cctcaatgcg gtgcttggct gctgcgtggt gagcacgttg
     2701 gtaattcatg ccttagagat caatctggac gctcttatta tttatgccaa tggcatcttt
     2761 attatgattt atctgttatg catgctggca ggctgtaaat tattgcaagg tcgttatcga
     2821 ctactggcgg tggttggcgg gctgttatgc gttctgttac tggcaatggt cggctggaaa
     2881 agtctctatg cgctgatcat gctggtgggg ttatggctgt ttctgccaaa acgaaaaacg
     2941 ccggaaaatg gcataaccac ataatccggc gtttcgacat taatcctggc gatcgtcttt
     3001 atgatcaagg cggtcgcggt catcatcctt tcgctgatac tcaccatcaa aagtattacc
     3061 gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga gaaaagcgca aatgtggcat
     3121 caacttcact gtcagatgct tttgcaccgg cggcaataaa agcagaagac cgaggaagtc
     3181 ggtgaaaaag cccggtaata aaagcagcaa accggcaata atcagcgaaa cacttttaat
     3241 catctccgcc gcagggtttt cacccgccgc cattttttgc tgcatcagca cgaaattctt
     3301 aaagccctgg ttacgtacca gtgacatacc gataactgac gtgaatataa ccagcacgag
     3361 ggtcagcaat acccccaata catgggcaac ctgaataaag attgaaatct caatatagac
     3421 ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt ttctcctgta atagcagccg
     3481 gttaaacccg gctacctgaa tgggttgcga atcgccttca gcttatattg tggtcattag
     3541 caaaatttca agatgtttgc gcaactattt ttggtagtaa tcccaaagcg gtgatctatt
     3601 tcacaaatta ataattaagg ggtaaaaacc gacacttaaa gtgatccaga ttacggtaga
     3661 aatcctcaag cagcatatga tctcgggtat tcggtcgatg caggggataa tcgtcggtcg
     3721 aaaaacattc gaaaccacat atattctgtg tgtttaaagc aaatcattgg cagcttgaaa
     3781 aagaaggttc acatgtcaaa caacattcgt atcgaagaag atctgttggg taccagggaa
     3841 gttccagctg atgcctacta tggtgttcac actctgagag cgattgaaaa cttctatatc
     3901 agcaacaaca aaatcagtga tattcctgaa tttgttcgcg gtatggtaat ggttaaaaaa
     3961 gccgcagcta tggcaaacaa agagctgcaa accattccta aaagtgtagc gaatgccatc
     4021 attgccgcat gtgatgaagt cctgaacaac ggaaaatgca tggatcagtt cccggtagac
     4081 gtctaccagg gcggcgcagg tacttccgta aacatgaaca ccaacgaagt gctggccaat
     4141 atcggtctgg aactgatggg tcaccagaaa ggtgaatatc agtacctgaa cccgaacgac
     4201 catgttaaca aatgtcagtc cactaacgac gcctacccga ccggtttccg tatcgcagtt
     4261 tactcttccc tgattaagct ggtagatgcg attaaccaac tgcgtgaagg ctttgaacgt
     4321 aaagctgtcg aattccagga catcctgaaa atgggtcgta cccagctgca ggacgcagta
     4381 ccgatgaccc tcggtcagga attccgcgct ttcagcatcc tgctgaaaga agaagtgaaa
     4441 aacatccaac gtaccgctga actgctgctg gaagttaacc ttggcgcaac agcaatcggt
     4501 actggtctga acacgccgaa agagtactct ccgctggcag tgaaaaaact ggctgaagtc
     4561 actggcttcc catgcgtacc ggctgaagac ctgatcgaag cgacctctga ctgcggcgct
     4621 tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga agatgtccaa aatctgtaac
     4681 gacctgcgct tgctctcttc tggtccacgt gccggcctga acgagatcaa cctgccggaa
     4741 ctgcaggcgg gctcttccat catgccagct aaagtaaacc cggttgttcc ggaagtggtt
     4801 aaccaggtat gcttcaaagt catcggtaac gacaccactg ttaccatggc agcagaagca
     4861 ggtcagctgc agttgaacgt tatggaaccg gtcattggcc aggccatgtt cgaatccgtt
     4921 cacattctga ccaacgcttg ctacaacctg ctggaaaaat gcattaacgg catcactgct
     4981 aacaaagaag tgtgcgaagg ttacgtttac aactctatcg gtatcgttac ttacctgaac
     5041 ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa tctgtgccga aaccggtaag
     5101 agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg aagcggaact tgacgatatt
     5161 ttctccgtac agaatctgat gcacccggct tacaaagcaa aacgctatac tgatgaaagc
     5221 gaacagtaat cgtacagggt agtacaaata aaaaaggcac gtcagatgac gtgccttttt
     5281 tcttgtgagc agtaacttaa aaataacaat ctaatatcaa cttgttaaaa aacaaggaag
     5341 gctaatatgc tagttgtaga gctcatcata gttttgctgg cgatcttctt gggcgccaga
     5401 ttggggggaa taggtattgg ttttgcaggc ggattggggg tgctggttct tgccgctatt
     5461 ggcgttaaac ccggtaacat cccgttcgat gtcatttcca ttatcatggc ggttatcgcc
     5521 gctatttctg ccatgcaggt tgctggcggt ctggactatc tggttcatca gacagaaaag
     5581 ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac cgatcgtgac ctatttcctg
     5641 actatctttg ctggtactgg caacatctct ctggcgacac tgccagttat cgctgaagtt
     5701 gcgaaggaac aaggcgttaa accttgccgt ccgctctcta ctgcagtggt atccgcgcag
     5761 attgcgatca ccgcatcgcc aatctcagcg gcagtggttt acatgtcttc cgtgatggaa
     5821 ggtcatggca tcagctacct ccatctgctc tccgtggtca tcccgtccac cctgctggcg
     5881 gttctggtga tgtccttcct ggtcactatg ctgttcaact ccaaactctc tgacgatccg
     5941 atttatcgca agcgtctgga agagggcttg gttgaactgc gcggtgaaaa gcagattgaa
     6001 atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc tgggcgtagt tggcgtggtt
     6061 atctatgcaa tcatcaacag cccaagcatg ggtctggttg aaaaaccgct gatgaacacc
     6121 accaacgcaa tcctgatcat catgctcagc gttgcaactc tgaccaccgt tatctgtaaa
     6181 gtcgataccg acaacatcct caactccagc accttcaaag caggtatgag cgcctgtatt
     6241 tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt ccaacaacat cgactggatc
     6301 aaagataccg ctggtgaagt gattcagggt catccgtggc tgctggccgt catcttcttc
     6361 tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa aagcactgat gccgatggct
     6421 ctggcactga acgtttcacc gctgaccgct gttgcttctt tcgctgcggt gtctggtctg
     6481 ttcattctgc cgacctaccc gacgctggtt gctgcggtac agatggatga cacgggtact
     6541 acccgtatcg gtaaattcgt cttcaaccat ccgttcttca tcccgggtac tctgggtgtt
     6601 gccctggccg tttgcttcgg cttcgtgctg ggtagcttca tgctgtaatg atcaatcgcg
     6661 gggcgttcac gccccgcttt ctttcccgcc gactaacatc cttcccccgt ccgttgtata
     6721 gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg tttatgcttg atgaaaaaag
     6781 ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg gatgaagcga cagcccagga
     6841 tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc gcgaccttga tccccggcgc
     6901 tacctctctc tattactggg aaggtaagct ggagcaagaa tacgaagtgc agatgatttt
     6961 aaaaactacc gtatctcacc agcaggcact gctggaatgc ctgaagtctc atcatccata
     7021 tcaaaccccg gaacttctgg ttttacctgt tacacacgga gacacagatt acctctcatg
     7081 gctcaacgca tctttacgct gattctgcta ctttgcagca cttccgtttt tgccggatta
     7141 ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc aagcctttgc ttttgatttt
     7201 cagcaaaacc aacatgacct taatctgacc tggctgatca aagacggtta ctacctctac
     7261 cgtaaacaga tccgcattac gccggaacac gcgaaaattg ccgacgagca gctgccgcaa
     7321 ggcgtctggc atgaagatga gttttacggc aaaagcgaga tttaccgcga tcggctgacg
     7381 cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt taactgtcac ctaccagggc
     7441 tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa ccgttccgtt aagcgaagtg
     7501 gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc agcaagagca gcacaccgcg
     7561 caattgccct tttccgcgct ctgggcgttg ttgatcggta ttggtatcgc ctttacgcca
     7621 tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc tgggtggtaa acagcggctc
     7681 tccactgcca gagcattgtt gctgaccttt atttatgtgc aggggatggc actgacttac
     7741 acggcgctgg gtctggtagt tgccgccgca gggttacagt tccaggcggc gctacagcac
     7801 ccatacgtgc tcattggcct cgccatcgtc tttaccttgc tggcgatgtc aatgtttggc
     7861 ttgtttactc tacaactccc ctcttcgctg caaacacgtc tcacgttgat gagcaatcgc
     7921 caacagggcg gctcacctag cggtgtgttt gttatggggg cgattgccgg actgatctgt
     7981 tcaccatgca ccaccgcacc gcttagcgcg attctgctgt atatcgccca aagcgggaac
     8041 atgtggccgg gcggcggcac gctttatctc tatgcgttgg gcatgggcct gccgctgatg
     8101 ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg gcccgtggat ggaacaagtc
     8161 aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct tcctgctgga gcgagtgatt
     8221 ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg tcgcattctt tggctgggcc
     8281 tttatcacca gcctacaggc taaacgcggc tggatgcgcg tggtgcaaat aatcctgctg
     8341 gcagcggcat tggttagcgt gcgcccactt caggattggg catttggtgc gacgcatacc
     8401 gcgcaaactc aaacgcatct caactttaca caaatcaaaa cggtagatga gttaaatcag
     8461 gcgctcgttg aagccaaagg caaaccggtg atgttagatc tttatgccga ctggtgcgtc
     8521 gcctgtaaag agtttgagaa atacaccttc agcgacccgc aggtgcaaaa agcgttagca
     8581 gacacggtct tacttcaggc caacgtcacg gccaacgacg cacaagatgt ggcgctgtta
     8641 aagcatctta atgtccttgg cctaccgaca attctctttt ttgacggaca aggccaggag
     8701 catccacaag cacgcgtcac gggctttatg gatgctgaaa ccttcagcgc acatttgcgc
     8761 gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg aggaaaatag cgtgcaacgt
     8821 gaagatgtac tgggagaagc cctgaaatta ttagaattac aagggattgc caacaccacg
     8881 ctggagatgg ttgctgaacg tgtggattat ccactggacg agctacgccg cttctggcca
     8941 gacaaagagg caatcctcta cgatgcgctg cgttatctta gccaacagat agatgtctgg
     9001 cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac aaaagctgct ggcacgttat
     9061 caggcgttat cggagtgcgt taaaaacaac cgctatccgg gctgtctatt tatcgctgcc
     9121 tgtacgtttt atcccgatcc tggccaccct attcatcaac tggccgatca gcaaaaaagc
     9181 gctgcctacg atttcaccca cgaactgtta accacgctgg aagttgacga tccggcgatg
     9241 gtagcaaagc agatggaact ggtgctggaa ggctgtttaa gccgaatgct ggtgaatcgt
     9301 agccaggcgg atgtcgacac tgcacatcgg ctggcggaag atatcctgcg cttcgcccgc
     9361 tgccgccagg gtggtgcatt gacctgacag aaacacagaa aagaagcgat ttgccgcaat
     9421 cttaagcagt tgaatcgctt ttactgaaat taggttgacg agatgtgcag attacggttt
     9481 aatgcgcccc gttgcccgga tagctcagtc ggtagagcag gggattgaa
//
LOCUS       DAZSNM010000157         9444 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZSNM010000157 DAZSNM010000000
VERSION     DAZSNM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.157"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1154
                     /locus_tag="ACHW42_003112"
     CDS             <1..1154
                     /locus_tag="ACHW42_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944825.1"
                     /translation="EDMCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKY
                     SAKDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNV
                     PKNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACE
                     AHSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFN
                     QSDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVM
                     GCKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKD
                     LFDIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1833..2051
                     /locus_tag="ACHW42_003113"
     CDS             1833..2051
                     /locus_tag="ACHW42_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFR8944826.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2269..2511
                     /gene="yiiF"
                     /locus_tag="ACHW42_003114"
     CDS             2269..2511
                     /gene="yiiF"
                     /locus_tag="ACHW42_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR8944827.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="ACHW42_003115"
     CDS             complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="ACHW42_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR8944828.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="ACHW42_003116"
     CDS             complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="ACHW42_003116"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR8944829.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="ACHW42_003117"
     CDS             complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="ACHW42_003117"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR8944830.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="ACHW42_003118"
     CDS             complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="ACHW42_003118"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7777..7779),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR8944831.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8558..9391
                     /gene="fdhD"
                     /locus_tag="ACHW42_003119"
     CDS             8558..9391
                     /gene="fdhD"
                     /locus_tag="ACHW42_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR8944832.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2312 a         2315 c         2448 g         2369 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggcta caccattcag attatgtaaa taaatatccc
     1621 tgaatataat atattcatag cacccataac tcatcctggg cgataactaa accatttttg
     1681 agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct cgcaaaaaca
     1741 aaatgacatg caaaaatccg cattttttta aatgcttttc cggcgtaatt ttcgcacata
     1801 cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
     1861 tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat
     1921 ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta
     1981 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
     2041 ataaggaatg aaaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt
     2101 attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca tcgtatctgg
     2161 aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcggcaa
     2221 gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat gaactctctg
     2281 gcaggtatag atatgggcag aattttactc gatttatcga atgaggtgat taagcaactt
     2341 gatgatctcg aggtgcagcg taatcttcct cgtgcggacc tattaaggga agcggtagat
     2401 caatacctga taaatcaatc gcaaacagca agaaccagtg ttcctggcat ctggcaaggg
     2461 tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg aggaatggta atgggaaaaa
     2521 atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg
     2581 ccttatccgg cctacaaagt catgcaaatt caatatattg aagtttattg gtaggcctga
     2641 taagcgcagc gcatcaggca atcatacgct tgtcatcagt gacataccca taaccttatc
     2701 gatgtgttgg agggaagaac gaggcaaaca gacaattgcg gcatgatctt gaatgggtaa
     2761 cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg cgacctgcgc
     2821 cacccggaaa acgtcagaga ttactctcct tcacccggaa acaggaacgg gttaatggaa
     2881 ctacgggcgt agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct
     2941 gccacggctt caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccgcag
     3001 tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt
     3061 ttgccgctct gctcgcagtt gctgcatttt acgcgcacta cgtgccattc ggtttcacac
     3121 aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc
     3181 ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg
     3241 ccggggatca gattggccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc
     3301 gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc catatcttcc
     3361 agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt
     3421 tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc
     3481 agaacgtgaa tatccagcgg gggcttgccc tgtgcgctgg cttctttaat gcgcgcggtc
     3541 agatccatct ccagcggatg gtcgtacagc accactttct gggcgtgggc gataagcgca
     3601 gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg
     3661 cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta
     3721 cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct
     3781 tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc
     3841 tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga
     3901 tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga
     3961 atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc
     4021 tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt
     4081 tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc
     4141 aaaagatatc atcccgattg attaggtttt gatgccagta acggaaaaac atgatgatga
     4201 acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc
     4261 ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg
     4321 ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca
     4381 cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc
     4441 ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg ccgcaaaggt
     4501 agccgcaaag ccaacagccg cgagcggttt ccagatgcct ttccagaatt tcacggtttc
     4561 gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag
     4621 cacgtacatg acgtgtgtac caccgacgcc tgccggatcg tacagacccg cattgtcgta
     4681 accgcgggtt ttcagctcag caacgcgctc gctcgccagc gttttcatcg actctttcgt
     4741 gccgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac
     4801 cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg
     4861 aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa
     4921 gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcaggca
     4981 gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt
     5041 ctgctccact tccgagaagc gcattaccgt ccacgatttg gcgcttaaat cattggggtt
     5101 gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc
     5161 cacctgacag gctttacagc cgatacaggt ggtaacgtcg atgagtttcg ccacttcttc
     5221 ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat
     5281 atcttgagat tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa
     5341 ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg aatggcgtta aagtattggc
     5401 aataaagcct tttttagcta cgccttcata gccccagtga atcggaatac cgatggtatc
     5461 gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt
     5521 gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc caagtttatt
     5581 cgccagcgac tccccgattt ccacaaactg ctctggctgc aaaatcgcgt tcaacagcgc
     5641 gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa
     5701 cttatcggct ttacccaatg cttcggcgtc gtctttaaag atacgtgcag ctgggttcga
     5761 gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc
     5821 cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg
     5881 catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg gaatatccca
     5941 gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc
     6001 ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc
     6061 ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt
     6121 gccttccggc gtccagctac cggcgaaaat ccagcagcca caggaggttg taccgtcatc
     6181 gcgcaattgg gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc
     6241 cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt cttccgactt
     6301 cggctcatgc ggaatggcgt agttccaggt catgttcagc acctgatccg ggttcgcgcc
     6361 accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga tctcaccgtc
     6421 agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc agcgacctga
     6481 gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga acacttcggt
     6541 ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt
     6601 ttcagtgttc agtgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac
     6661 tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac
     6721 cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg
     6781 caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat
     6841 catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg
     6901 ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt
     6961 catgtaacct ggcagactct gcgacagtag ccccaggtcc gtcagcccct gaatattgga
     7021 gtgaccgcgc agggcgttaa cgccgccgcc ggccatcccc atattgccga gcagcagctg
     7081 gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc
     7141 atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt
     7201 caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga
     7261 aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt
     7321 ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact tgcgtttttc
     7381 cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct
     7441 ggcgttggta taggcttcgg tgtattcgtg gttgaatttt tcattgttca gcaggtacag
     7501 caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc
     7561 taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat
     7621 tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat
     7681 caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc
     7741 aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac
     7801 cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga
     7861 cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc cttcggcgtt
     7921 ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc
     7981 ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt
     8041 cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc
     8101 gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt
     8161 tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt
     8221 attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct
     8281 gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa
     8341 gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt
     8401 tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc
     8461 aaaggtccag aattggctca atttccctcc atctaggaag ggtttgtaac agaataccat
     8521 aatgttggtg tgtatgttct tatctggtta agagaaagtg aaaaaaacac agcgaaaaga
     8581 aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga
     8641 tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc tggtctacaa
     8701 cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg
     8761 tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca tggatgtcgt
     8821 tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct ttatggggtt
     8881 gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gcgtggagca
     8941 acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt ttgatctcaa
     9001 caaactggat gatgcattac gtcatctcaa cgattttcag ccagtggggc gactgactgg
     9061 ttgtactcac gccgctgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga
     9121 cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag
     9181 ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc
     9241 ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc ttgctgtaga
     9301 agtggctgag cgctgtaatc tgacactggt aggtttttgt aaaccgggtc gggcaacggt
     9361 ttatacccat ccgcagcgtt tgagcaatta attaaaataa gtcctgcgat atatattaaa
     9421 ttgcaggact tattcatttc gtga
//
LOCUS       DAZSNM010000158         9439 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZSNM010000158 DAZSNM010000000
VERSION     DAZSNM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9439
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.158"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..778
                     /gene="amiA"
                     /locus_tag="ACHW42_003120"
     CDS             <1..778
                     /gene="amiA"
                     /locus_tag="ACHW42_003120"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR8944833.1"
                     /translation="AIAKDEPLKTSNGHSKPKAKKSGGKRVVVLDPGHGGIDTGAIGR
                     NGSKEKHVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEIAHKHGADLFMSIH
                     ADGFTNPKAAGASVFALSNRGASSAMAKYLSERENRADEVAGKKATDKDHLLQQVLFD
                     LVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVLVETSFITNP
                     EEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSKKR"
     gene            782..1681
                     /gene="hemF"
                     /locus_tag="ACHW42_003121"
     CDS             782..1681
                     /gene="hemF"
                     /locus_tag="ACHW42_003121"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR8944834.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(1687..2739)
                     /gene="eutR"
                     /locus_tag="ACHW42_003122"
                     /pseudo
     CDS             complement(1687..2739)
                     /gene="eutR"
                     /locus_tag="ACHW42_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(2785..3285)
                     /gene="eutK"
                     /locus_tag="ACHW42_003123"
     CDS             complement(2785..3285)
                     /gene="eutK"
                     /locus_tag="ACHW42_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR8944835.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(3298..3957)
                     /gene="eutL"
                     /locus_tag="ACHW42_003124"
     CDS             complement(3298..3957)
                     /gene="eutL"
                     /locus_tag="ACHW42_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR8944836.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(3967..4035)
                     /locus_tag="ACHW42_003125"
                     /pseudo
     CDS             complement(3967..4035)
                     /locus_tag="ACHW42_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5139..5930
                     /locus_tag="ACHW42_003126"
     CDS             5139..5930
                     /locus_tag="ACHW42_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944837.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            6650..7045
                     /locus_tag="ACHW42_003127"
     CDS             6650..7045
                     /locus_tag="ACHW42_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR8944838.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            7063..7326
                     /locus_tag="ACHW42_003128"
     CDS             7063..7326
                     /locus_tag="ACHW42_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944839.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            7349..7891
                     /locus_tag="ACHW42_003129"
     CDS             7349..7891
                     /locus_tag="ACHW42_003129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944840.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            8030..8317
                     /locus_tag="ACHW42_003130"
     CDS             8030..8317
                     /locus_tag="ACHW42_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944841.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            8356..8562
                     /locus_tag="ACHW42_003131"
     CDS             8356..8562
                     /locus_tag="ACHW42_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944842.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            8874..9439
                     /locus_tag="ACHW42_003132"
                     /pseudo
     CDS             8874..9439
                     /locus_tag="ACHW42_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816255.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
BASE COUNT         2618 a         2289 c         2296 g         2236 t
ORIGIN      
        1 agccattgcc aaagacgaac ctttaaaaac cagcaacgga cacagcaagc cgaaagccaa
       61 aaaatctggc ggcaaacgtg tcgttgttct cgatccaggc cacggcggaa ttgataccgg
      121 agcgatcgga cgcaacggtt cgaaagaaaa acatgtggtg ctggcgattg ctaaaaacgt
      181 ccgttccatt ttgcgtaatc atgggattga tgcgcgttta acgcgttctg gcgatacgtt
      241 tatcccactt tacgatcgcg ttgaaatcgc ccataaacat ggcgcagatc tgtttatgtc
      301 aattcatgcc gatggcttta ccaacccgaa agctgccggt gcttcggtat ttgccctctc
      361 taaccgtggg gcaagtagcg caatggcgaa atacctgtct gaacgcgaaa accgcgccga
      421 tgaagttgcc ggtaaaaagg cgactgacaa ggatcaccta ttgcagcaag tactgtttga
      481 tctggtgcaa accgatacta ttaaaaacag cctgacgctt ggctcgcata ttctgaaaaa
      541 gattaagccg gtgcataaac tgcacagccg caacaccgaa caagccgcgt ttgtggtgtt
      601 gaaatcgccg tcggttcctt cggtgctggt ggaaacctcg tttatcacca acccggaaga
      661 agagcggctg ttaggcacgg cggcgtttcg tcagaaaatc gccacagcga ttgctgaagg
      721 cgtgatcagt tatttccact ggttcgacaa ccagaaagca cattcgaaaa agcgataagt
      781 tatgaaaccc gacgcacacc aggttaaaca gtttctgctc aaccttcagg atacgatttg
      841 tcagcagctg accgccgtcg atggcgcaga atttgtcgaa gatagttggc agcgcgaagc
      901 tggcggcggc gggcgcagtc gggtgttgcg taatggtggt gttttcgaac aggcgggcgt
      961 caacttttct cacgtccacg gtgaggcgat gcctgcttcc gccaccgctc atcgcccgga
     1021 acttgccggg cgcagtttcg aggcgatggg cgtttcactg gtagtgcatc cgcataaccc
     1081 gtatgttccc accagccacg cgaatgtgcg tttttttatt gccgaaaaac cgggtgcaga
     1141 gcccgtttgg tggtttggcg gcggcttcga tttaacccct ttctatggtt ttgaagaaga
     1201 cgccattcac tggcaccgca ccgcccgtga cctgtgccag ccatttggtg aagacgttta
     1261 tccccgttac aaaaagtggt gcgacgatta cttctacctc aaacatcgca acgaacagcg
     1321 cggcattggc gggctgttct ttgatgatct gaacacgcca gatttcgacc actgttttgc
     1381 ctttatgcag gcggtaggca aaggctacac cgacgcttat ttaccaattg tagagcgacg
     1441 taaagcgatg gcctacggcg agcgcgagcg caattttcag ctctaccgtc gcggtcgtta
     1501 tgtcgagttc aatctggtct gggatcgcgg cacgctgttt ggcctgcaaa ctggcgggcg
     1561 caccgagtcg atcctgatgt caatgccgcc actggtacgc tgggagtatg attatcagcc
     1621 aaaagatggc agcccagaag cggcgttaag tgagtttatt aaggtcaggg attgggtgta
     1681 actccctcac ccccattccc gcatccgctg atgcaacgtc aacgacggct tctcggcaaa
     1741 cagctgctgg taatccgtgg caaattgccc cagatgccag aatccccact gcatggcggc
     1801 gtcttttacc gttgtgcttt gcgaccacgg gcttatcagt tcgcggcgta cgacgttcag
     1861 gcgaatgcgt ttcagccacg cgtttgggcc aatgcctaaa atagcgtgaa acgcgttttg
     1921 tagcgtgcgg cgactgacat gcagttgatt acacaaatcc agcaccgtca ccggttcgga
     1981 catgttttcc agcacatatt cacgggcgcg ggaaagcaac cgacggtaac tctgatgact
     2041 gatgctttcc gccgtcacca ttggttgcgc ttcttccagc atcgccccca tcgccattag
     2101 caaattatcc cccagcactt ttcgcactgc aggctgatgg agattttccg ggttctcgca
     2161 aaacgtcgcc agcgcctgtt ggacaaagcc ccacagcgcg gctttatgct gctctttcac
     2221 ttccagcgcc gactggctac gcagcatatg taatacccga tccggattgt gcaaaaagtt
     2281 agcctgacag gtgatgacat cttcagacag cactacgccc aggatcgtat aatcgtccgg
     2341 cgtacttaat tcaaactcgg ttccaccggg gcgcgtcgct atttctgcgc ttcccagaca
     2401 ttgcgaaccg ataaatccct gctcaccgcg cgtcgccgga atgccaaacc agaacgagtt
     2461 cggccagacc aggcacgact gacgcagcgc cagaccggtg tattcacgaa aaacctgaat
     2521 atcatcgagt agaatttccg taaattcacc atgaaacttg cccggatgca gctgatcgta
     2581 aatctgctgc taggcggtaa tcgtcaaagc atgttcatag acatccgttg tctgtcgttg
     2641 atgaacatta tccacttcga ccttcggcgt gagcttcagg ttttcgggta agggttcatg
     2701 ataaagatgg tgcaaattgg ctgtacgggt ctttttcatg atgttaatgc cgggtgttgt
     2761 aggacacccg gcacctccga caggttaatg gggcttgaga cgataacgac tactgcgttt
     2821 acgtaacgtc ccggcagaaa agagctgttc gagcgcattt ctggcttttt ccagcggcca
     2881 gccaaagtgg gcggccactt ctcctgccgt cattccctga catactgatg ttaacagcgc
     2941 caacaactcg tcagcagatt ccgcgacgat aactggcgcg tcgggttcct ttacgggttg
     3001 cttcggctgg cggttaaagc cagtaaccag ccactgggtg tcatcgtctg gccgaccgat
     3061 ctccttacgg ctgatgacac gcccggtacg catcgcggca gcacaacctg cgtccagcgc
     3121 cgcacgacac gccgccagat cgccttccac caccagcgtt aagcgaccag ggtcaagcac
     3181 ttcgtgactg agcagacgaa cgttagctgc tttgagcatg gcatccgctg catctatcgc
     3241 ggcgaccatg ccgtccactt ccagcaatcc cagtgcattg atcatcggca acctccgtta
     3301 cgcacgctgg attggattac gcgcgatttc cagcactgca tcggtaaagg cgttacaggc
     3361 tgctttacac gcggcctggc taccggttaa aaatgctgcc gagtagttgg tttcagacgg
     3421 tggcgggacg taggtcacca gctgtacgtc ggcagatttc aacgcggcat caatgccgta
     3481 ggtcgcttcc agcggcggtg ccaccagata cgccatcgga tcgccaagcg tgatcccggc
     3541 ggttgatgaa agataagaac cggtacgcga aaccacatgc gccaggaagg cggtgttttc
     3601 tgcgtcattg gcccactgga aagctgcgcc attttcaata tgtgcaacca tcgcatccag
     3661 accggcacgc acttccgccg ggtttggccc accaagcata atcagcactt caccggcagt
     3721 cggtgacggg ccgtgtgccg ctcccgcata cagcgagcga ccatatacca cttcgaccat
     3781 cgcttgtttg gtcgcttcgt cagccgcaat gtaggttacg tcgtcagaat cggcagaaat
     3841 cagcccgagg ctacgaatat gcggcggcaa ttttagttca cgcgcaaatt cagcgttaac
     3901 ggaggcaatc acccgcatgg cggttaccga cggtcgaatc aaatctaaag ctggcatgat
     3961 gcctccttat cgggtcatgt tgatgccgga tgctttctgc tccagcatac gtttcgccaa
     4021 atccacaatg acggcacagc agcacgaaag agaagcaaaa gtcagctacc tggtacacta
     4081 ggcagcgggt cagcaaaggg gggcaaatgc cccttttttc atgtcagata atcagatcat
     4141 tacctcaata aactaataat cacatcgcgg ttacaaccag tttgatagtg atagctcaaa
     4201 cccagacgtg gcgctggctg caagcaaaat aagccgcaaa aatgcgaccc catgtttact
     4261 agccgcaaaa atgcggtcct atttgccaga accttttatt attcaattgg ttagtgcaag
     4321 aaaaagcgtg attactgttc aagaagtatc cagaaaggtc atgattttga cgctgaaaaa
     4381 aaataggtgg gcatttttgc ccactcacta gccgcaaatt tgcccactca ctagccgcat
     4441 ttttgcggcc caaagtaata aatacagaga aataataaat acagatcagc gccaagattt
     4501 ttcgtctttt cggcggaatc atgagactag tgcttacgtc ctgataacct ccagagcaga
     4561 ccttaacttc tgtgcagcag gactacgctc attgccgctc ggctaggtca aatcctcacg
     4621 ataaagctgt tcggattgtg accataatgt taaaggactc tttaaatcat tccttgacga
     4681 attaactagc tggcttaagc ggaatagcat gcaggataaa aacaaaacca gcttagcagt
     4741 ggggagagct tcgcttcgct cgtctcctgg ggccaatgct caatgaaacg acgtaaggca
     4801 cggagcgctc accataggta gacacataaa catctgagag cgcaccttct atcgcgctac
     4861 gcgctcaata aaaaccaacg acaccgccac actgcaagac caatggtttt tatcgcgtat
     4921 acagaccaga caggaaacag cagcaaagca gcgtatagcc aggtgcagac agcacactca
     4981 cacgacgcac agcaggctta agtgatcact caggaaactt tatccccgcc gccgatcacc
     5041 gcgaactatc atgaccgcgc caaattcgaa aggaatggcc catagaacgc gatgaacaca
     5101 cataagcgct acacttactt aacgtcgcca cagagcgatt gagagcgctt aaaatcgatg
     5161 cgatttcatt aaatttttct cactcgcctg aatggtggcg atcaggactg atcgcaacat
     5221 tgccccgcct cgatattgag cgcataatag atacatacat taagcggaaa agagataagt
     5281 ttattgtgaa aatcaggggg ttacatatca tgcgcatatc agaacaacaa cggcagatcc
     5341 tcgactatct ggcaaaaaac gaatcaatca ccgcacgaca agccgcagat ctcgtttatg
     5401 gtggcaatgt tacccgcgtc caggttgacg ccgcccgccg ctcactcatg acgatggtta
     5461 gtaatgggct aatcaggaaa caaggccgcg ctttcgtagc tgaaagacat atcaatcacg
     5521 cttatagcca ggacgttgaa gaattgcgcc gccagatcct ttgggtattc agcaaacagg
     5581 gtcaggagta tttcctttct gaacacccaa atttcgccgc caaagagtta acagtaagcg
     5641 gattgtcgtg cgcactggat tatcttgtcg gtgaaggttg cgttacttac aagcagctat
     5701 ggcacgccct gaatacgctt gtagaagatg gtttactcat tgccacgcgt gagaacatcg
     5761 aatactggat aatgccgcgt tcaggtcgcc gcaatcatgc acggtatatt tcaggcaaca
     5821 gatacagaac gccggacttt aatccgacac aggaagatcg ggaccgtgcc gaaatgatgt
     5881 gcagggaaca ggagaaaaga attatggata agttattttc gatgcagtga acacaagcgg
     5941 gcgcaatgcc cgtttttatt gccttattcc acgcctgacg aatcaatttg atcacacctg
     6001 agaaaatgca tagccagaag ttaaggccgt tatattgcga ttgggagcgt tttaaacggt
     6061 atcaatttaa ttaattctga tgtggtgatt tgcaggcaaa aaaaacccgc cggagaagcg
     6121 ggaaatagag acatatcgtt gtagttatta acagatcact ttgaggtcga gggcggctaa
     6181 ccctcgctaa tattatcccg ctattacctt gcgggtgcaa ataatctttt attgctttga
     6241 tgatttaata aatagcactt tttgctaaca aaaaccgctg tcaatatatg gccaaaaatg
     6301 tcaacagaaa aaataaggtt tataatatta agtatcacat gggtaatggg ttcagggaat
     6361 cggaatccac cgcctcacgg aaattcaaaa tttgcttctt ctttcctccg tgggcggctt
     6421 ctttttcgta tcgaggtatt tatgaaagca ataacattag aacagttcgc agcaatggcg
     6481 aatgtatcga ccagaacgct gaggcgctgg ctaaaaaaac catcaaccgg agtccctttg
     6541 ccatttaccc cacgcggtac tcgccaatta ttctgggagc acgaatgcaa agaatggttg
     6601 tacagaacag aacgataata atcacgcatc acattttgag gtaaacatca tgaccaatat
     6661 cgaaagatta gaacgctgtg ccgatattat gcgacgcaga tggatttatg accctgaagc
     6721 gggtttattg tttagccgtg aaacaaaaga cgttatcaaa ggcagcatga ccaataaagg
     6781 gcatttggtt gtaacggtac atgaaggtaa attcaaagtg acactcacct atcagaaagc
     6841 ctgctatgta tacacatacg gcgcttatga cgagacatta tatgaagtgc accacgttaa
     6901 tttgaacaaa caggataacc gcatcaagaa tattcgcctg atgccgaaag aaaagcaccg
     6961 ccggatacat aaaaacgtcc gcaaacttat tcggctgggc cacacacaat ggcttcaacc
     7021 gcaattaatt gaacaatcac tataaacaca ggagtaaata ccatgcattc attagacagt
     7081 tattttcagc gcacgacagc ccctaaatca gccgcccagg aacgccgtga ggaattccat
     7141 gagaaagtga tgcgttctgc tgattatatt gcggacaagt tcgtggaaac ggttcgtcca
     7201 cttgttgatg aagtagctga taaacttcag tctgaaatgc cggaagatat ggaaggcact
     7261 gcaaagcggc gactcatttg cgaattaagt cgtcgttttg gcgtgtcaat aagcgctttt
     7321 aaataatcga aaaaaggggg gaaagataat ggacgcattt gaggtactgg caaataaagg
     7381 cgctgaactg gtagcacagc gtgataaaac cgccaacgaa ggggaacgcg cactgctaaa
     7441 taaacagatt aaagcgatcc gtatggccca gtttaaatta atcagtaatg aagttatcga
     7501 aacaattaaa ccgcagatcc cacaaatcgt tgctgacgct attaaagccg ccggactgga
     7561 gaaacgcatt gccgggctaa aaacgaacgc agagaaaatg gagatgtaca aacagagcgg
     7621 tgctccgaat cccgatgagt acttctcacc ggatgttgag tttatggcgc aggttgagga
     7681 acgtctgggt agctgtctca ccgaggaaca gcgtcgctat tttgatggag tggatagttc
     7741 ggcaggtatt gacctgaact cgtatttcgg tcgtgaaatt gagcattttg atgccgcccc
     7801 gtctatgccg gagccagaac cagaaatggc caccgatcct gaagctgagc aacggaaacg
     7861 cgtattcaat gcgataggga ttcggtactg atcggataaa agaaagccgg gaattacccc
     7921 ggctgactta acaatgtgac caatgaacaa atcggacaag acaaacagga taagcagtct
     7981 cgaaacaatg ttcgggaact atattagcac aagtccggag aaaatcatca tgttggcact
     8041 acgcatcatg cagggcatcg ctaagaccct cgcagaacat gtcttagacc tgaaacattc
     8101 gccactgagc aaacaggcca tgaagcgcca aacgcttcgt ctgtgggctg aatattcgct
     8161 tggaactatc aataaaatca tcgacatgaa atcggggccg agcaaccaga gcgcggagga
     8221 aatggagttc atcaggcgtt taattctcat tcgcagagac attcatagcc agttacacag
     8281 cgtgggaatc gatattaatg atgggacggg cgattaattt tcggacaagt actatgcgcg
     8341 cgcgaggagc tgaacatgaa gcaagatatt gcagacaggc tggagatact cgaaggccag
     8401 cgggcagaag cgaaacaact acgtaagcag gccagaagag cgcacaggaa caacgaagca
     8461 gagttactaa ccaaatacat cagtttcacc aattactgca tctacgagtg ttacaaggaa
     8521 gatgcagagg actggctgga ttcattgcca gaacagtact gagcagaata acaccacgtc
     8581 atgttgttct gaggtgagca tatcacctct gttcaggtgg ccaaatttag tgtgccactt
     8641 cagcatcacc gttgaaatca caataacgat ttttttgtaa atatgctctc acatatgtag
     8701 gaatagacca ggttggcatt gaaacacatc taccatgacc ataataaagt aattttttga
     8761 tatgtttatg gttttatatt aattaaaata tatctaaatt tgatagtttt aaaattgcaa
     8821 aatccagatt gtgtttgtaa tgtttaatct gattgtattg agtggcgttt attatgaaaa
     8881 ataaatcagg taattcttgt tatagagaag cagtcgatat tcatagctca cttgagcttt
     8941 catttatatt gcataaatat catttgcgtg tagatgagaa gaaatctcga aatgtattca
     9001 gcgccatacg tgatgagctc atccttgatg gggattcacg acaaaactta gctactttct
     9061 gtcaaacatg gcttgatgat gaaatccgtg agttgcttaa tttagctatt gataaaaaca
     9121 tgatttataa agatgaatat ccacaaatgg cggaacctga aggtcgctgt gtgcgtatgc
     9181 tttccgattc gtggagtttc cccgattcgc gacacacctt aggttgctca accattggtt
     9241 catctgaagc tgctatgctc ggccgcttgg cgttaaaatg gcagtggtgt aaaaaacgcg
     9301 aagtacaagg taaatcaacc gaacctgatt tgcgatcctg tgcaaatatg ctggcataaa
     9361 ttctgccgtt actgtgatgt gaaattgtgt gaaattccac ttgatgatca tcacctgata
     9421 atgaatgcta aagaggttt
//
LOCUS       DAZSNM010000159         9413 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZSNM010000159 DAZSNM010000000
VERSION     DAZSNM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9413
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.159"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..331)
                     /locus_tag="ACHW42_003133"
     CDS             complement(<1..331)
                     /locus_tag="ACHW42_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944843.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            493..549
                     /gene="ysgD"
                     /locus_tag="ACHW42_003134"
     CDS             493..549
                     /gene="ysgD"
                     /locus_tag="ACHW42_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR8944844.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="ACHW42_003135"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="ACHW42_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR8944845.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="ACHW42_003136"
     CDS             complement(1692..2072)
                     /locus_tag="ACHW42_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFR8944846.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..2466)
                     /gene="yigG"
                     /locus_tag="ACHW42_003137"
     CDS             complement(2086..2466)
                     /gene="yigG"
                     /locus_tag="ACHW42_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HFR8944847.1"
                     /translation="MLRMFIPTSNGKISRHRYIFLFILINFIFAFLIIFFNDGEAGFL
                     VIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITMIAEDFACSGNGS
                     MIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="ACHW42_003138"
     CDS             complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="ACHW42_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR8944848.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3503..3970)
                     /gene="yigI"
                     /locus_tag="ACHW42_003139"
     CDS             complement(3503..3970)
                     /gene="yigI"
                     /locus_tag="ACHW42_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR8944849.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4135..5004
                     /gene="pldA"
                     /locus_tag="ACHW42_003140"
     CDS             4135..5004
                     /gene="pldA"
                     /locus_tag="ACHW42_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR8944850.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5137..6966
                     /gene="recQ"
                     /locus_tag="ACHW42_003141"
     CDS             5137..6966
                     /gene="recQ"
                     /locus_tag="ACHW42_003141"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR8944851.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7030..7650
                     /gene="rhtC"
                     /locus_tag="ACHW42_003142"
     CDS             7030..7650
                     /gene="rhtC"
                     /locus_tag="ACHW42_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR8944852.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7712..8332)
                     /gene="rhtB"
                     /locus_tag="ACHW42_003143"
     CDS             complement(7712..8332)
                     /gene="rhtB"
                     /locus_tag="ACHW42_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR8944853.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8443..>9413
                     /gene="pldB"
                     /locus_tag="ACHW42_003144"
     CDS             8443..>9413
                     /gene="pldB"
                     /locus_tag="ACHW42_003144"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR8944854.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIV"
BASE COUNT         2360 a         2321 c         2401 g         2331 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggcaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatgcgcca ttataggtcc tagatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctggctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaaa taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc ggctatcatt gtgatgatag agatgatata
     2221 tactgctaat gtaccaaaaa cataaatttt tatatatatg aaaccactat cacggagtcg
     2281 ttggcaattc atgttgatga cgagataatg gagtgcgatg gtagagacta taacaagaaa
     2341 acctgcttcc ccatcgttaa aaaagataat aagaaaggca aaaatgaaat ttattaaaat
     2401 aaataaaaat atatatcggt gtctggaaat cttaccgtta gatgttggga taaacatacg
     2461 taacataaat tttacatcct tgtgtgagtc tcctgtcagc atggcaatat gcccactctc
     2521 atacaagagt cggcatattt tttcagaata tatttatttt ttatttggac gttctacgct
     2581 gcgtataaat cgcgtccatc acaaaaattg ccagcgccac ccaaataaag gtgaaagtca
     2641 ccatcttatc ggcacccggt ttttcaccat aaaacgtcac agccagcagg aacatcagcg
     2701 tcgggccaat gtactgaaaa aagcctaacg ttgagagacg caggcgcgtg gcagcggcag
     2761 taaaacacaa cagcggtacg gtagtgacaa taccggcggc gatcagcagt aaattcagcg
     2821 acatcgggtt ttgccccata tggctggttg aactgtcggc aatagcaaac aggtaaatcg
     2881 ccgccacggg cagcagccac atggtttcaa ttaacatgcc ggtttgtgct tctaccgcaa
     2941 tcttcttgcg caccaggccg tagaaggcaa aactaaatgc cagccccagc gcgataatag
     3001 gtagcgaacc aaaagtccac agctggacca acacgccaca tatcgccaga atcaccgcca
     3061 gccattgcat ccggcggaat cgctcgccga ggaaaatcat ccccagcaca atgttcacca
     3121 gcgggttaat aaagtaacca aggctcgctt ccagcatatg gtgattgttc accgcccaga
     3181 taaacagtag ccagttgcca ccaatcagta cggcagagac tgccagcata aaaattttct
     3241 gtggcgtctg aatcagcgtt tttaaatagg accactggcg gcaaatgctc atcagcacca
     3301 ccataaagaa aaacgaccag atcacgcgat gcgtcaggat ctcatcggcg ggcacgtagt
     3361 aaatcagctt gaagtatgct ggcgcaatac cccaaataaa ataagcggca agagcgagta
     3421 atacgccctg ccgcgtttgt tttgcatcca tcgggaatac tcatttttaa ttggtaacag
     3481 cagtttacct gcttttatgt cttcaaccta ccatataggt ggcggtggca ctggcaatat
     3541 aaagctgttc ttcattgtgt aattcaacgc gggcgacggc gactttattg cctgcacgca
     3601 acaggctact ggtagcagta aaacgctcgc ccctgcctgg gcgcagataa tcaacgcgaa
     3661 gatcaatggt ccccatccgc gatagccgct ggcgtagttc atcttcactg atggtttcgt
     3721 ggcgggttaa ggtacttccc acgcacacca gaccggcggc gacatccagc gccgacgcaa
     3781 tgactccgcc gtgcaaaatg ctttgcgccc agttacccac catcatcggc tgatttttaa
     3841 aggccagctg tgcgaactct ttttcgtaac gctccagttc catccccaat gcgcggttaa
     3901 atggcatgtg ataaacaaac atctcaccca ctaatttcag ggcttgttca gcggtcagta
     3961 cggcagacat atcatcctta cacttcattg gttaatgaaa tgttgatttt atgcttcttt
     4021 gttgttggtt tctactttag gaagggataa ctaacggcaa tggagttaag tatgtaaaat
     4081 agcccgcaga aaaacattca ccttatcaat aattcgttac ggagaacacg accgatgcgg
     4141 actctgcagg gctggttgtt gccggtgttt atgttgccta tggcagtata tgcacaagag
     4201 gcaacggtga aagaggtgca tgacgcgcca gcggtgcgtg gcagtattat cgccaatatg
     4261 ctgcaggagc atgacaatcc gttcacgctc tatccttatg acaccaacta cctcatctac
     4321 acccaaacca gcgatctgaa taaagaagcg attgccagtt acgactgggc ggaaaatgcg
     4381 cgtaaggatg aagtaaagtt tcagttgagc ctggcatttc cgctgtggcg tgggatttta
     4441 ggcccaaact cggtgttggg tgcgtcttat acgcaaaaat cctggtggca actgtccaat
     4501 agcgaagagt cttcaccgtt tcgtgaaacc aactacgaac cgcaattgtt cctcggtttt
     4561 gccaccgatt accgttttgc aggttggacg ctgcgcgatg tggagatggg gtataaccac
     4621 gactctaacg ggcgttcaga cccgacctcc cgcagctgga accgccttta tactcgcctg
     4681 atggcagaaa acggtaactg gctggtagaa gtgaagccgt ggtatgtggt gggtaatact
     4741 gacgataacc cggatatcac caaatatatg ggttactacc agcttaaaat cggctatcac
     4801 ctcggtgatg cggtgctcag tgcgaaagga cagtacaact ggaacaccgg ctacggcggc
     4861 gcggagttag gcttaagtta cccgatcacc aaacatgtgc gcctttatac tcaggtttac
     4921 agcggctatg gcgaatcgct catcgactat aacttcaacc agacccgtgt cggtgtaggg
     4981 gttatgctaa acgacttgtt ttgatgaacg gttgagtggt tggcaaatct ggaatccagc
     5041 atccaggatt accctctcag agactaaaag cattgcagtt tctcgcgcag gcgctgaaaa
     5101 tagcgcctgt ttttatttca ggcaatcggg gtgaatgtgg cgcaggcgga agtgttgaat
     5161 ctggagtccg gagctaaaca ggttttacaa gaaacctttg gctaccaaca gtttcgcccc
     5221 ggccaggaag aaattatcga cactgtgctt tccggtcgcg attgcctggt tgttatgccc
     5281 actggtggcg gaaaatccct ttgctatcaa attcctgcct tattgctaaa cggccttacc
     5341 gtggttgttt caccgctgat ttcgttgatg aaagatcagg tggatcaact acaggccaac
     5401 ggcgtggcgg cggcgtgcct taactcgacg caaacccgcg aacagcaact tgaagtgatg
     5461 acaggctgcc gcaccgggca aattcgctta ctgtatatcg ccccggaacg cctgatgctg
     5521 gataactttc ttgagcatct ggcgcactgg aatccggtgt tattagccgt cgatgaagcg
     5581 cactgtatct cccaatgggg tcacgatttc cgcccggaat atgccgcgct cggtcagttg
     5641 cgccagcggt tcccgacgct gccgtttatg gcgctgaccg ccacagccga cgacaccacg
     5701 cgccaggata tcgtgcgcct gctggggctg aacgatccgc tgattcaaat cagcagtttt
     5761 gaccgtccga atattcgcta catgctgatg gagaaattca aaccgctcga tcagttgatg
     5821 cgctacgtgc aggaacagcg cggtaagtct ggcattatct actgcaacag ccgggcgaaa
     5881 gtagaagaca ccgctgcgcg cctgcaaagc aggggtatta gcgcggcggc ctatcatgcc
     5941 gggctggaaa ataatgtccg cgccgacgtg caggagaaat tccagcgcga tgatctgcaa
     6001 attgtggtgg cgacggtggc gttcggcatg ggcatcaata aaccaaacgt tcgcttcgtg
     6061 gtccactttg atattccgcg caatatcgaa tcctattatc aggaaaccgg tcgcgccggg
     6121 cgtgatggcc tgcctgcgga agcgatgctg ttttacgatc cggctgatat ggcgtggctg
     6181 cgccgttgtc tggaagagaa gccgcagggg cagttgcagg atatcgagcg ccacaaactc
     6241 aatgcgatgg gcgcgtttgc cgaagcgcaa acttgccgtc gtctggtatt gctgaactat
     6301 tttggcgaag ggcgtcagga gccgtgcggg aactgcgata tctgcctcga tccgccgaaa
     6361 cagtacgacg gttcaaccga tgctcagatt gccctttcca ccattggtcg tgtgaatcag
     6421 cggtttggga tgggttatgt ggtggaagtg attcgtggtg ctaataacca gcgtatccgc
     6481 gactatggtc atgacaaact gaaagtctat ggtatgggcc gtgataaaag ccatgaacat
     6541 tgggtgagcg tgatccgcca gctgattcat ctcggcctgg tgacgcaaaa tattgcccag
     6601 cattctgccc tacaactgac agaggccgcg cgcccggtgc tgcgcggcga atcctctttg
     6661 caacttgccg tgccgcgtat cgtggcgctc aaaccgaaag cgatgcagaa atcgttcggc
     6721 ggcaactatg atcgcaaact gttcgccaaa ttacgcaaac tgcgtaaatc gattgccgat
     6781 gaaagtaatg tcccgccgta cgtggtgttt aacgacgcaa ccttgattga gatggctgaa
     6841 cagatgccga tcaccgccag cgaaatgctc agcgttaacg gcgttgggat gcgcaagctg
     6901 gaacgctttg gtaaaccgtt tatggcgctg attcgtgcgc atgtcgatgg cgacgacgaa
     6961 gagtagtcag cagcataaaa aagtgccagt atgaagactc cgtaaacgtt tcccccgcga
     7021 gtcaaatgta tgttgatgtt atttctcacc gtcgccatgg tgcacattgt ggcgcttatg
     7081 agccccggtc ccgatttctt ttttgtctct cagaccgctg tcagtcgttc ccgtaaagaa
     7141 gcgatgatgg gcgtgctggg cattacctgc ggcgtaatgg tttgggctgg gattgcgttg
     7201 cttggcctgc atttgattat cgaaaaaatg gcctggctgc atacgctgat tatggtaggc
     7261 ggtggcctgt atctctgctg gatgggttac cagatgctac gcggtgcact gaaaaaagag
     7321 gctgtatctg cgcctgcacc acaggttgag ctggcgaaaa gcgggcgcag tttcctgaaa
     7381 ggcttactga ccaatctcgc caatccgaaa gcgattatct attttggttc ggtattctca
     7441 ttgtttgtcg gcgataacgt tggcactacc gagcgctggg gcattttcgc gttgattatt
     7501 gtcgaaacac tggcgtggtt taccgtcgtt gccagtctgt ttgccctgcc gcaaatgcgc
     7561 cgtggttatc aacgtctggc gaagtggatt gatggttttg ccggggcgtt atttgccgga
     7621 tttggcattc atttgattat ttcgcggtga tgccagacgc gtcttcagag taagtcggat
     7681 aaggcgttta cgccgcatcc gacattattt ttcacgcatg cctcgccgat gctaacagcg
     7741 ctcccaccag cataaacaac gagccgaaaa tcttattcag cgccttcatc tgctttggtc
     7801 ctttaatcca tagagcaatc ctttgagcaa gggtggcgta accgatcatc acaataatat
     7861 cgaccacaat agtggtgacg ccgagcacga tatactgcat cagttgcggc tgttgcggca
     7921 tgatgaattg cggaaatagc gccgccagaa acacaatact tttgggattg gtgagattca
     7981 caaaaactgc gcgctggaac aaatgtcgac gcgattgagt agaggccagc gatttaagat
     8041 caattgcgcc agcggcgcgc cactgctgga ttcccagcca aatcaagtaa gccgcgcctg
     8101 cccactttaa cacttcaaac gcaatcactg agcgggaaaa tagcgtcccc aaccccacgc
     8161 caaccagcac aatatgaatc gccagtccgg tctgaagccc agcaatagac gccaccgcgc
     8221 cgcgataacc gtggttgagc gaggtggtca tagtgttgat tgcaccagag cctggcgaca
     8281 ggcttaaaat gatcgatgtc agcaggtagg caaaccacca ttctaaggtc atgatgaact
     8341 cccggtgtgt ctatttttgt gccacaatac gctactgtcg cagcgttgtg tcaggcacgc
     8401 ttaaaaaaac gattttacgt ggtttaagag gcagattacc cgatgtttca gcagcaaaaa
     8461 gactgggaaa caagagaaaa cgcgtttgct gcttttacca tgggaccgct gactgatttc
     8521 tggcgtcagc gtgatgaagc agagtttact ggtgtggatg acattccggt gcgctttgtc
     8581 cgttttcgcg cacagcacca tgaccgggtg gtagtcatct gcccggggcg tattgagagc
     8641 tacgtaaaat atgcggaact ggcctacgac ctgttccatt tggggtttga tgtcttaatc
     8701 atcgaccatc gcgggcaggg acgttccggt cgcctgttag ccgatccgca tctcgggcat
     8761 gttaatcgct ttaatgatta tgttgatgat ctggcggcat tctggcagca ggaggttcag
     8821 cccggtccgt ggcgtaaacg ctatatactg gcacattcga tgggcggtgc gatctccaca
     8881 ttatttctgc aacgccatcc aggtgtatgt gacgccattg cgctaactgc gccaatgttt
     8941 gggatcgtga ttcgtatgcc gtcatttatg gcacggcaga tcctcaactg ggccgaagcg
     9001 catccacgtt tccgtgatgg ctatgcaata ggcaccgggc gctggcgcgc gttgccgttt
     9061 gctatcaacg tactgaccca cagcagacag cgatatcgac gtaacttacg cttctatgct
     9121 gatgacccaa cgattcgcgt cggtgggccg acctaccatt gggtacgcga aagtattctg
     9181 gctggcgaac aggtgttagc cggtgcgggt gatgacgcca cgcaaacgct tctcttacaa
     9241 gctgaagagg aacgcgtggt ggataaccgc atgcacgacc gtttttgtga actccgcacc
     9301 gccgcgggcc atcctgtcga aggaggacgg ccgttggtaa taaaaggtgc ttaccatgag
     9361 atcctttttg aaaaggacgc aatgcgctca gtcgcgctcc acgccatcgt tga
//
LOCUS       DAZSNM010000160         9412 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZSNM010000160 DAZSNM010000000
VERSION     DAZSNM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.160"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            70..222
                     /gene="hokE"
                     /locus_tag="ACHW42_003145"
     CDS             70..222
                     /gene="hokE"
                     /locus_tag="ACHW42_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR8944855.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            573..725
                     /gene="hokE"
                     /locus_tag="ACHW42_003146"
     CDS             573..725
                     /gene="hokE"
                     /locus_tag="ACHW42_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR8944856.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(847..1467)
                     /gene="entD"
                     /locus_tag="ACHW42_003147"
     CDS             complement(847..1467)
                     /gene="entD"
                     /locus_tag="ACHW42_003147"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR8944857.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="ACHW42_003148"
     CDS             complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="ACHW42_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR8944858.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4125..5327
                     /gene="fes"
                     /locus_tag="ACHW42_003149"
     CDS             4125..5327
                     /gene="fes"
                     /locus_tag="ACHW42_003149"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR8944859.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5330..5548
                     /gene="ybdZ"
                     /locus_tag="ACHW42_003150"
     CDS             5330..5548
                     /gene="ybdZ"
                     /locus_tag="ACHW42_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR8944860.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5545..9390
                     /gene="entF"
                     /locus_tag="ACHW42_003151"
     CDS             5545..9390
                     /gene="entF"
                     /locus_tag="ACHW42_003151"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR8944861.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2049 a         2477 c         2598 g         2288 t
ORIGIN      
        1 cctggggctg ccactccaaa ccagacaatt tggatggtag tcccttcttc gcatggaggt
       61 aatataaaca tgctgacgaa atatgccctt gtggcagtca tagtgctgtg tttgacggta
      121 ctgggattta cgcttctggc cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat
      181 attgagttca gggctgttct cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa
      241 tccccgcata tccggttgtc aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg
      301 cggtcagtga tggctgcggg cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga
      361 agtccgaaac aggactcact gtataaataa acagctattt tgctaaggaa gggtaagata
      421 acggcgggtg cctgaagctt tccggtttca ggttaactct gaggtttgga aagatgaagc
      481 cccaagagat atttctatca atcctggggc tgccactccg aacctcaaca actcggatgg
      541 tagtcccttc ttcgcatgga ggcaatataa acatgctgac gaaatatgcc cttgtggcag
      601 tcatagtgct gtgtttgacg gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg
      661 agttcaccgt aaaggaacgt aatattgagt ttaaggctgt tcttgcttac gaaccgaaga
      721 agtagccgtt gtgcggggag taatccccgc atatccggtt gtcaggtcag agtggtaagg
      781 cacctgcttt acactttcgc ccgcagtcag tgatggctgc gggcgaatcg taccagatgt
      841 tgtcaattaa tcgtgttggc acagagttat gactattttt tcttttatct gccagtgcac
      901 agcaaacatc tcattctcac gatgaatgat gacctgctgt ttattccagc taattatctg
      961 atagtccaga aaacctgcat cagtttggat ctcacttgcc ttaaatgcgc tctctttggc
     1021 ggaaaatgca agtgtcagcg ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg
     1081 ttctgctggt gtaataatgt tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc
     1141 ttcgatatca acgccaatcg gttgacggga aaccacggcc aatgccgtag ccccgcagtg
     1201 gctaatactg ccgtatacct ccgcaggcca caccggttgg cgtagctcgc cgattgcggg
     1261 cacacattta tagccatatt cccgcaaggc ataaacagca gcgatccgtc cggctaaatg
     1321 ctctgtttta cgtttacgtc cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa
     1381 atcctgctca caaaaattcg ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa
     1441 ggggagggag gtatgcgtag ttttcatatc gaccatattc gagactgatg acaaacgcaa
     1501 aactgcctga tgcgctacgc ttatcaggcc tacgtgatcc tgcaatatat tgaattggca
     1561 agatctttgt aggccggata aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt
     1621 gtcaacaatc tgacgttagc atcagaagtg agtattaatg ctcatatacc acgtacgtcc
     1681 cggctcgtta taggtatacg cgccggcacc ggcgatatag ttggctcctg ccagatcgcc
     1741 cgtggtctgg gcattacctg cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc
     1801 ggtcagactg acattcttcg tcacatccca ggtcgcgctc agacccacaa tgctgtaagg
     1861 actgatttct ttggtttccg ggccaaccgc tggctgacct ttatagttgt acttcttcgg
     1921 ctgctgcttg ccgtaccagg tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca
     1981 gctcagcgtt gagttcaacg tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt
     2041 gttttcactc ttcagcatat aagtgatgtt attggtccac atcaccgttt cgctaactgg
     2101 tacgtttaac gatccttcca gaccttcaac cactgctttc ggcacgttat cccactgata
     2161 gagatcagtg ccgactgcgt tttgccctac agccacatag cccgcttcaa tcttattgcg
     2221 ataatcgtta cggaaccagg tgacgcccgc cagccagccg tcgcgtttga actccagacc
     2281 aatctctttg ttgatgctgg tttctacttt caggtcatcg ttaccttgca gatagcagcc
     2341 gcccgcgctg gcatagcagc cctgaccttt actgtagaga atgtagttcg ggttagtctg
     2401 gtataggctc ggcgctttat aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc
     2461 taaaccttgc gatatgttca gcgccgggct ccagttattg ccgacaatac tgtgatgatc
     2521 gaaacgcagc cccggcgtta cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa
     2581 cagcgagaaa atttctgctt ttgaatacgg gctacggtcg gtggcactca cgccatcaat
     2641 agcgccaccg gtattcgttc cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg
     2701 ttgctgattc cactccgtac ccagcgtcag cgtctggtta acgaggaaat caatcggcag
     2761 gttaacttcg ctgtgcagca tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc
     2821 tttttcgtta aatttccctt cggtaccgcc cgccagacct tccggaatac gcgagttacg
     2881 ggtgtgttca tactgcaccc agttgctggt ggtcacgccg ttatcccagc caccgttcca
     2941 ggtcagcgcg tagttctggc gatacaggcg gttggtttca tcgccatatt tcgagcgagt
     3001 gtaagcgtca gagttggtgt tctgggtatc gcccgcatac aggttaccct ggcggctgta
     3061 acccgcttcc agttccagcg attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat
     3121 atctttattg atcacccctt cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg
     3181 cgcggactga tggccctggt tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc
     3241 atacaagcgg aagctgaatt cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt
     3301 ggtggcacct tcctctttat gttctggcgc attgaaatat gcgtcccagg agccgtgcca
     3361 ctcgccgctg ccttttttgg taatgatgtt aaccacgccg cccgccgcgc cgttgccata
     3421 acgcgcagct gccggaccac gcagaacttc aatacgttca atcatttcag gtggcaccca
     3481 ggaggtatca ccacgggtat cgcgctcgcc acgccagccc tgacgcaccg agttacggct
     3541 gcttaccggc ttgccgtcaa tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc
     3601 aatctgtcgg ttattcccac gctgaccact ggtggagtta ccggtcaggt taacgcctgg
     3661 catggtacgg atgatctccg acacatcgcg ggcaaccggg tttttgcgga tttcatctgc
     3721 ggtgatggtc gaaacgccag gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt
     3781 atcgtcatgt gaaacaggtg tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc
     3841 cagattgacc aacaaggcca gggaatgaat cttcttgttc attgttttat tcctgcattt
     3901 ttgccacgaa ttgcaactgt cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc
     3961 gaaaaccttt gatcctgaaa gacacgcagt gcagttggtt aattaatgtc cgcgcttccc
     4021 acggcgcgcc attacgctat tgcaaatgca aatagttatt aataacatta tcaatatatt
     4081 tctgcaatca atgaaaaatt gcacagtaaa catggggtta tggtgtgacg gcgttaaaag
     4141 taggaagtga gagctggtgg cagtcgaaac atggcccgga atggcagcgt ctgaatgacg
     4201 aaatgtttga ggtcactttc tggtggcgtg atccccaagg ttctgaagaa tactcgacga
     4261 taaagcgcgt atgggtctac atcactggtg tgaccgatca tcatcagaac agccagcccc
     4321 agtcgatgca gcgaattgca ggtactgatg tctggcagtg gacgacacaa ctcaatgcca
     4381 actggcgcgg cagctactgc tttattccca ccgaacgcga tgacattttt tctgcaccat
     4441 cccccgatcg cctcgaatta cgtgaaggct ggcgaaaact attaccccag gcaatagccg
     4501 atccgctgaa tccacaaagc tggaaaggcg ggctagggca cgctgtttct gcactcgaaa
     4561 tgccgcaagc gcctctgcaa ccgggatggg attgtccgca agcgccagaa ataccagcca
     4621 aagaaattat ctggaaaagt gaacggttga aaaattcacg tcgtgtatgg atttttacca
     4681 ccggcgatgt aacagcagaa gaacgcccgc tggcagtttt gctcgatggc gaattttggg
     4741 cgcaaagtat gcccgtctgg cctgtgctga cttcgctgac ccatcgtcag caacttcctc
     4801 ccgccgtgta tgtgttgatc gacgctatcg acaccacgca ccgcgcccac gaactgccgt
     4861 gtaatgcgga tttctggctg gcagtacagc aagagttatt acccctggtg aaagttattg
     4921 ccccttttag cgatcgcgcc gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt
     4981 ccgcgctgta tgccggactg cactggcctg aacgctttgg ctgtgtatta agccagtcag
     5041 gatcgtactg gtggccgcat cggggcgggc agcaagaggg cgtgttactt gaaaagctaa
     5101 aagctggtga agttagcgcc gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc
     5161 cgatgatcat gcgggccaat caggcgctgt atgcgcaatt acaccctata aaagaatcca
     5221 ttttctggcg tcaggttgac ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc
     5281 aggggttaat cgacctctgg caaccacttt tccatgacag gagttgaata tggcattcag
     5341 taatcccttc gatgatccac agggagcgtt ttacattttg cgcaatgcgc aggggcaatt
     5401 cagtctgtgg ccgcaacaat gcgccttacc ggcaggctgg gacattgtgt gtcagccgca
     5461 gtcacaagag tcctgccagc agtggcttga agcccactgg cgtactctga caccggcgaa
     5521 tttcacccag ttgcaggagg cacaatgagc cagcatttac ctttggtcgc cgcacagccc
     5581 ggcatctgga tggcagaaaa actgtcggat ttaccctccg cctggagcgt ggcgcattac
     5641 gttgagttaa ccggagaggt tgatgcgcca ttactggccc gcgcggtggt tgccggatta
     5701 gcgcaagcag atactttgcg gatgcgtttt acggaagata acggcgaagt ctggcaatgg
     5761 gtcgatgatg cgctgatatt cgaactgcca gaaattatcg acctgcgaac caatattgat
     5821 ccgcacggta ctgcgcaggc attaatgctg gcggatttgc aacaagattt gcgcgtcgat
     5881 agcggtaaac cactggtctt tcatcaactg atacaggtgg cagataaccg ctggtactgg
     5941 tatcagcgtt atcaccattt actggtcgat ggcttcagtt ttccggccat tacccgccag
     6001 atcgccaaca tttactgcgc attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg
     6061 cctttcgctg atgtagtgga agagtaccag caataccgcg aaagcgaagc ctggcagcgt
     6121 gatgcggcat tctgggtgga acagcgtcgc caactgccac cgcccgcgtc actttctccg
     6181 gcacctttag cggggcgcag cgcctcggca gatattttgc gtctgaaaat ggaatttacc
     6241 gacggggaat tccgccagct ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc
     6301 cttgcgctga cggctttgtg gctggggcga ttgtgcaatc gcatggacta cgccgccgga
     6361 tttatcttta tgcgtcgact gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac
     6421 gttttgccgc tgggtattca tattgcggcg caagaaacgc tgccggaact ggcaacccga
     6481 ctggcaaccc gactggcagc acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc
     6541 gaacaaattg tccgtgacag cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta
     6601 ctcaatatta aggtatttga ttaccaactg gatattcctg gtgttcaggc gcaaacccat
     6661 accctggcaa ccggtccggt taatgacctt gaactggccc tgttcccgga tgaacacggt
     6721 gatttgagta ttgagatcct cgccaataaa cagcgttacg atgagccaac gttaatccag
     6781 catgctgaac gcctgaaaat gctgattgcc cagttcgccg cggatccggc gctgttgtgc
     6841 ggcgatgtcg atattatgct gccaggtgag tatgcgcagc tggcgcagat caacgccact
     6901 caggttgaga ttccagaaac cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca
     6961 ccggatgctc cggcgctggc agatgcgcgt tacctgttca gctatcggga aatgcacgag
     7021 caggtggtgg cgctggcgaa tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg
     7081 gcggtggcac taccgcgctc ggtctttttg accctggcac tccatgcaat agttgaagct
     7141 ggcgcggcct ggctaccgct ggataccggc tatccggacg atcgcctgaa aatgatgctg
     7201 gaagatgcgc gtccgtcgct gttaattacc accgacgatc aactgccgcg ctttagcgat
     7261 gttcccaatt taaccaacct ttgctataac gccccgctta ccccccaggg cagtgcgccg
     7321 ctgcaacttt cacaaccgca ccacacggct tatatcatct ttacctctgg ctccaccggc
     7381 aggccgaaag gggtaatggt cgggcagacg gctatcgtca accgcctgtt gtggattcaa
     7441 aatcattatc cgctcacagg cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat
     7501 gtctcggtgt gggagttttt ctggccgttt attgccgggg ctaaactggt gatggctgaa
     7561 ccggaagcgc accgcgaccc gctcgctatg cagcaattct ttgccgaata tggcgtaacg
     7621 accacgcact ttgtgccgtc gatgctggca gcatttgttg cctcgctgac tccgcaaacc
     7681 gctcgccaga gttgcgtgac gttgaaacag gttttctgta gtggtgaggc cttaccggct
     7741 gatttatgcc gcgaatggca acagttaacc ggcgcgccgt tgcataacct gtatggcccg
     7801 acggaagcgg cggtagatgt cagctggtat ccggcttttg gcgaggaact ggcacaggtg
     7861 cgcggcagca gtgtgccgat tggttatccg gtatggaata cgggtctgcg tattcttgat
     7921 gcgatgatgc atccggtgcc gccgggtgtg gcgggtgatc tctatctcac cggtattcaa
     7981 ctggcgcagg ggtatcttgg acgacccgat ctgaccgcca gccgctttat tgccgatcct
     8041 tttgctcctg gtgaacggat gtatcgtacc ggagacgttg cccgctggct ggataacggc
     8101 gcggtggagt acctcgggcg cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa
     8161 ctgggcgaaa tcgatcgcgt gatgcaggcg ctgccggatg tcaaacaagc cgttacccac
     8221 gcctgtgtga ttaaccaggc ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat
     8281 ctggtatcgc aatcgggcct gccgttggat accagcgcat tgcaggcgca gcttcgcgaa
     8341 acattgccgc cgcatatggt gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc
     8401 gccaacggca agctggatcg caaagcctta ccgttgcctg aactgaaggc acaagcgcca
     8461 gggcgtgcgc cgaaagcggg cagtgaaacg attatcgccg cggcattcgc gtcgttgctg
     8521 ggttgtgacg tgcaggatgc cgatgctgat ttctttgcgc tgggcggtca ttcgctactg
     8581 gcaatgaaac tggcagcgca gttaagtcga cagtttgctc gtcaggtgac gccggggcag
     8641 gtgatggtcg cgtcaaccgt cgccaaactg gcaacgatta ttgatggtga agaggacagc
     8701 tcccggcgca tgggattcga aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg
     8761 ttttgtttcc atccggcgtc cggttttgcc tggcagttta gcgtgctctc gcgttatctc
     8821 gatccacaat ggtcgattat tggcattcag tcgccgcgcc ctcatggacc catgcagaca
     8881 gcgacaaacc tggatgaagt ctgcgaagcg catctggcaa cgctacttga gcaacaaccg
     8941 cacggcattg cggcgcggct gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg
     9001 gatacctggc cgccagagac gcaaaactgg caggaaaaag aggctaatgg tctggacccg
     9061 gaagtgctgg cggagattaa ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt
     9121 acttcaacgg agttgtttac caccattgaa ggcaactacg ctgatgctgt gcgcctgctg
     9181 acgactgctc atagcgtacc gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg
     9241 cttcaggaag gtatgagccc cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc
     9301 tatcgtcagg attgtgcgca tgtggatatt atctctccag gggcgtttgt aaaaattggg
     9361 ccgattattc gcgcaacgct aaacaggtaa attaatatta tctataaacc ca
//
LOCUS       DAZSNM010000161         9392 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZSNM010000161 DAZSNM010000000
VERSION     DAZSNM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.161"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="ACHW42_003152"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="ACHW42_003152"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR8944862.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="ACHW42_003153"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="ACHW42_003153"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFR8944863.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="ACHW42_003154"
     CDS             3115..3399
                     /locus_tag="ACHW42_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR8944864.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACHW42_003155"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACHW42_003155"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR8944865.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACHW42_003156"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACHW42_003156"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR8944866.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACHW42_003157"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACHW42_003157"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR8944867.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="ACHW42_003158"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="ACHW42_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR8944868.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2245 a         2479 c         2388 g         2280 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaaa ta
//
LOCUS       DAZSNM010000162         9351 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZSNM010000162 DAZSNM010000000
VERSION     DAZSNM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9351
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.162"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(52..531)
                     /gene="xdhC"
                     /locus_tag="ACHW42_003159"
     CDS             complement(52..531)
                     /gene="xdhC"
                     /locus_tag="ACHW42_003159"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR8944869.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="ACHW42_003160"
     CDS             complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="ACHW42_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR8944870.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="ACHW42_003161"
                     /pseudo
     CDS             complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="ACHW42_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4129..4884
                     /gene="actS"
                     /locus_tag="ACHW42_003162"
     CDS             4129..4884
                     /gene="actS"
                     /locus_tag="ACHW42_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR8944871.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4963..5036
                     /locus_tag="ACHW42_003163"
     tRNA            4963..5036
                     /locus_tag="ACHW42_003163"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4995..4997,aa:Gly,seq:ccc)
     gene            5171..6781
                     /locus_tag="ACHW42_003164"
     CDS             5171..6781
                     /locus_tag="ACHW42_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8944872.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6813..7232
                     /locus_tag="ACHW42_003165"
     CDS             6813..7232
                     /locus_tag="ACHW42_003165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944873.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKVSWR"
     gene            8351..9097
                     /locus_tag="ACHW42_003166"
     CDS             8351..9097
                     /locus_tag="ACHW42_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR8944874.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
BASE COUNT         2383 a         2309 c         2384 g         2275 t
ORIGIN      
        1 ttaaacacat aacctcagtg aatccggcct gaattcaggc cggatatcct tttacttcgt
       61 tttctcgcaa tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt
      121 tcccgccagt ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag
      181 cattgccgtg gtagccataa tcaggccagg cgtacaaaac ccgcactgca ctgtgccgga
      241 tttcgcataa gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt
      301 gcggatctct tttccttcag cccaggcggc aaggtataag caactgtcta ttgctgtgcc
      361 gtcgaccaac accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag
      421 cagtccttgt tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa
      481 aggcatcccg ttaatggtgc attcggtggt aattgtttcg ctgtgattca ttgcaatttt
      541 ccccccgccg cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc
      601 agacgaaact ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat
      661 tcgctgatag cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca
      721 tgttggcagc gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa
      781 ttgccgttat ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg
      841 cgcatggcat atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga
      901 aaggcgacga ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca
      961 ttaatcggga cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt
     1021 agcgttggcg tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta
     1081 gcgacgttac ggatctgcgg tccagcaatg aacgtggccg cagcacataa cgccgggaga
     1141 tgacgttgag ttataggatc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt
     1201 agcgagccat cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca
     1261 atatggcgat aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccaccggcg
     1321 agcagtttgg cctgcgggtt gtcagccagc aggttgatgg catcggcaag ggttgctgcg
     1381 cgatggtaag aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa
     1441 ctcttcatat aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat
     1501 cttcaccgcg ttacgaatag cagcggcaac aggaattatg ggtggctcac ccagtgactt
     1561 atgtccgtat gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag
     1621 atccggcatg gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt
     1681 agcatcgatg atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg
     1741 tacctgacct tctgccagca gtggattaag aatatgccct gaatcatgaa cgttgaggat
     1801 gcggttgatg gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc
     1861 aaacgccggt gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg
     1921 ttcagggtgg tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg
     1981 tcgttcaacc agcacgatat ggccttttat cagggtcaaa ttcatcgctg actgatgtag
     2041 catgactgcg gcgtgagcga tgattttctc ttttaataat agtgccgcac tgcgcagcgc
     2101 aggcgcggca acatagctct ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacgtc
     2161 ggtatcttgt gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac
     2221 catttgcgag aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt
     2281 gatggttcca tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca
     2341 ggtgttagag gtgtagctaa aacaggcgac gccaacgccg cggcgcaaat tgccttgctg
     2401 gttctggcat tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca
     2461 ctccggcaac cctgcgctgc aaatacgttt gcccgtgagc ggattagcat ctccttcgcg
     2521 ggcggcgttg cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc
     2581 aagcatagac tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc
     2641 cgaggggagg ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag
     2701 gtaagcgact ttattccccc ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc
     2761 ggtgttagac agaacatcca gactataacc tttcaaggtt ccgtcgcggt tcacgcccat
     2821 ttgcccgtca atagtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct
     2881 aagggaaact ttcaccggaa tgccgccaag cttgctggtc aggaatgccg ccattggctc
     2941 ttccagtaca tcctgtttat taccaaaacc gccaccgaca aacggtttga tgactcgtac
     3001 gcatgaccag ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg
     3061 ggtgctggaa acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct
     3121 ttccatgtga caatgttgaa taacgggagt ctgatagtgc ccctgtacct ggtagtcggc
     3181 ggcatcgatt gtttgttgga cattgcccgt cgacatcgtg ctttgtttca gtaaattgcc
     3241 gccgttatgg attggtgcag cgtcttctgt cagcgccgct tctggcgtgg tgataacggg
     3301 taattcttgc cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg
     3361 ggccacgacg atggcaacgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg
     3421 atcggcggta tcgcgcttgt tttcgtcaag tgtctaggca tgcccagctg tagcgaatgg
     3481 aatatcaggc acatcttccc aagtaaaaat cgccagtacg cccggtaaac ttctggcttg
     3541 ttcatcatta atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcgta
     3601 acacatgccc gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat
     3661 ggcatcgacg cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat
     3721 cccctcgatt tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta
     3781 cgccagaaac gacgataagc gttaggggag gaaggaagtg tgatgacgtt aaaaaaacac
     3841 agatatgata cgaaacagga ctctgaatag ggacagtgat acgtatcaat atgagtggcg
     3901 ataaaatcaa tgtgataaat caggattatt acgcatcagg atgggaaata gggtaaaaag
     3961 tcaggaactt cgatacctct acagcgaata ttttgctgat gatacttccc tttgccaaat
     4021 gaaattaacc tctttctcta ttaccacgtt tttccagaag caagagattg ggtatcaaga
     4081 ggctggctgc tatgatatgg cgtcttgttt tttaagcgag gaaagatttt gagtgcggga
     4141 cgcctgaata aaaaatctct gggtatcgtg atgtttttat cggttggact gctattggcg
     4201 ggctgttcgg gtagcaaatc atccgataca ggaacgtatt ccggctccgt ttacaccgtg
     4261 aaacgggggg atacgctata tcgtatttcg cgcaccacgg gaaccagcgt aaaagagctg
     4321 gcgcgactga acggcatttc ccccccttac accattgaag ttggtcagaa actaaaactg
     4381 ggtggggcga aaagtagcag tagtacacgt aaatcaaccg ccaaatcaac gactaaaacc
     4441 gcatcggtta caccgtcatc agcggtaccg aaatcttcct ggccgccagt agggcaacgt
     4501 tgttggttat ggccaacgac agggaaagtt atcatgccgt attcgacagc agatggcggc
     4561 aataaaggga ttgatatctc agctccacgg ggtacaccta tttacgccgc gggtgcagga
     4621 aaggtggtgt atgtgggcaa ccagctgcgt ggctacggta atctcatcat gattaaacac
     4681 agtgaagatt acattacggc ttacgcccat aatgacacga tgctggtaaa taatgggcaa
     4741 agcgtgaagg ctgggcaaaa aatcgccact atggggagca cggatgcggc atctgttcgc
     4801 ctgcatttcc agattcgtta ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg
     4861 cagggcagca agccaaaatg ctgatggcga attaatcagc agtcagcagc gcggcacttg
     4921 ctaaggagag cgtaaggttt ataatgcctt acgcatctcg aagcgggcgt agttcaatgg
     4981 tagaacgaga gcttcccaag ctctatacga gggttcgatt cccttcgccc gctccatgac
     5041 acttcttaaa gtctactaac ttactgaatc ctaagggata cggcttaacg ccttccgata
     5101 gttttactca ctgtaaccaa cactgtgact aactttgttg aagtagtgag cagacctaag
     5161 gtctcagtac atgctcaagt caagaaccta cctttatcag cgtaatggtg tcttttacat
     5221 ccgtcttagg atgaagacca ctagtcgcct gaccgcttcg cttccttctc acaaccgtta
     5281 taaactagcg tcagtatctt tacggaccaa ggatagacgt accgctatgg ctcactcacg
     5341 gcacatcaag tcagcactta aagcaatcca tgcagacaac cctaacgcct cttatgagga
     5401 gctacgggag cacctgaaga ccattgtcga gtgggaactt agtgtcagcc gtgatgacct
     5461 gaacgacccg gagtcctacc agctttacgt tgaccagtac gatgacatta agtccaacct
     5521 tcgggaagct gtagcgaccg agcgtctgac tgtagaccag catcgctata ttaatgacgt
     5581 tatcggtgtg cttaaggcct gtcaggatag actcaatggc gatagctctg gcctattgtc
     5641 ttaccttgag cctgagactg gcagtctaag accgtccgtc tccctatctg tattggcgga
     5701 acctgaagtc cctgaaccta aagcactgac cctcgcgtct ctcattgagc agtacgagca
     5761 ggagaacgcc cagaactgga agccagcgac cctaagcgaa aaccgagcgt cacactctac
     5821 gctaatcgag atattcgact atctggatat tcaggatgtc ggcaaggcga cccgtgcaga
     5881 tatgctcaga gttcgtgaag tcctccagca gctaccgaag aaccgcaagc aacgctttaa
     5941 gtctatgccg ctgtctgact tgctgaaccg ggagtctaag actgactgtc tggacgtcgt
     6001 taccattaac aataaatatc ttatcaagat ggctgctgtg tttaagtggg cggtacgcaa
     6061 cgacttgata gctaagaacc tgaccgaagg tcttgagctg aaagtccctc agcgtaaagc
     6121 ctccgacgct cgtgatgcgt tcagtcctga gcaggtaggg caactactgg tcgcagctaa
     6181 agcgtactcg cagaagacct caggtaagcc ataccattat tacgtcaccg ctctggctgc
     6241 gattacaggt gcgagactta acgaggtggc ccaacttcag gttaaagatg tcaggaccac
     6301 tgaggctggg accgttttta tccatatcaa cgaagacgat agcagcctgc cgggtaagag
     6361 tatcaagaac gcccacagtg accgctgtgt cccgttggta gatggtgctt atggttttgt
     6421 attggctgac tttatgtcac tcgtggaaga ccgtagaaag actgagggtg ataatgctat
     6481 ggtgttcaat ggcctcaagc taatgaagaa cggctacggt gagcaggtta gcaagtggtt
     6541 taaccgaacg ttgctgccca aagtcctcgc tgaccgtagc ggcttagctt ttcactcgtt
     6601 tagacataca gttgcgaccc agttaaaaca acatggggtc gagttagcgt atgctcaggc
     6661 gattatgggt catagctcag ggtctataac ttacgaccgt tacgcgaaag aggtcgaggt
     6721 ggaaacgctg aaagaaaaat tagcggagtc attgtcagta aaaaaaatag acggaaagta
     6781 atcgcatagg gatagcggaa tgtgaggacg taatggtcaa agaattttta gttagcaaag
     6841 aaaataagcc ttcatatgaa tgtatgaata ctgctgaaac ttgtttgggg ctggctggaa
     6901 tggcttcgga agattggcaa cgtgagcatc attgccgcac aggtataata tttactgctt
     6961 ttgctattga ggcaatgttc atcttctata gaaaacaggt tgaccccggc tacgataaga
     7021 cgcagaaaga gtgcagaaag acaatgcaca agaatacgtt aaaattgtgc ggtataaaca
     7081 attatatggg gacaaaacct taccaaatta tcaaagagtg tcttgaggta agagacgcta
     7141 ttgctcacgg cgattcgtat acttcgagct ttaatttctc ggcggatcat ttggataacc
     7201 aagacgatat tgcaaaagtg tcctggcggt aaactctgaa caatttcggg gattaactgt
     7261 tgagcgcctt gcaaaataca ttgaaatggc aaagcatatt gaccatgaga tttctgataa
     7321 tggttacagg ccttctgaag gttaccttcc agcagccgag cgcagaagtc tgatgttagc
     7381 ttttggcgtc agtggtgtgt caatgtggcc cagtgcttga gcgataatac acctcactat
     7441 agaggaatgg acccttaact atcactatag gggagagacc ctaagtcccc accttaatag
     7501 cagtcacgac ccatacacac attcattaag tctcaggctt ccggtagcgg accgattgga
     7561 aaccatccgc agacgcctag tgttaaatca ccttggcctg agaccattcc ttttaacgta
     7621 agctatcttc ccttttgaca ggcggggagc agtagaccac atccgacttt aagttggctc
     7681 tgtgataatc gctgcgcgga taactgaaga accaagacac cattactgag gtctgccatc
     7741 catttgtgga caggcggaaa ctcaaagagg ttagatcagt ggtgtctgag gttggagctt
     7801 tgagtcttga ccagcaggct ggaggagacc atgagtttat cttgttatgg attgtgttat
     7861 cacaattcca ataagaagaa ctttaagtcc agcctccact cccgccaacc cgaagtaaaa
     7921 ttatggtcgc tatgatgtgg tctccatgct tcgggtctta ctggaagtac tgaacacttt
     7981 cgcgtcaagt tctccgaagt tcattcctgt ttgactaaac tatcactata gggaccatat
     8041 ctcgcctctg acctcctgag agacggcaag cgagaaaccg tggtccacgc tagcaagcta
     8101 gccaccagcc taaggcaaga cagcctctgg tcagcctgtg tgttctttgg tctcgtgact
     8161 taagtctatc accacacacg ctcggacatg ctgtcgaacc ttcatctcta tggtcatcgc
     8221 agaccactaa ccccaattac ctcaagtcat tctgtttgcg tcctgttgcc tgctggtctc
     8281 cctttacgtg ggggcttgag taggtcagtg gggtgcgact tcagggtgca aacaaaagga
     8341 gtacacgtaa atgtcccaac aagaaatcag catcattaac ctcgaccagc ttgtgtccat
     8401 gacctcagtt gaaattgctg agctgacagg aaaagaacat aaacacgttt tacgagatat
     8461 tcgtaatatg gtcgaagagc ttaacggggc caaaactgag cactgctcaa ctttgagcag
     8521 cgagcttaac gggtccaaat ttgggctcgt tggcgaggaa gtatacaagg acgcgaaagg
     8581 tgagagccga acaatgtatc gtctcgaccg taagcatact ttcattctgg tcgctggcta
     8641 ttccgttcac ctccgcgcca agtgttacga ccatattcag acgttagagc gtcgagtcct
     8701 tcaactggaa gaccagaaga agcgagcagc cattcagtcc gctaaccgcc gaggcgtgac
     8761 ttggggcgac tactgcaaaa cctatggtct gcccgctcag aagctaatga ccgcacttct
     8821 ccagcatcgt ggcctctttc gtaagaaccc aatcagtaac gagtggtctg taaatccgaa
     8881 gtattccgac tgctttcgta tcattaagcc gtcagaccag aagttctcag caggtgggta
     8941 taacttccgg ttcaacgcta aggggctgga ggtcttcggt aagcctgaga tggttgacaa
     9001 gatgcgaggc atccttattg cattcactgg caccgaccag cagaagcaag agcacctcct
     9061 gaagctggca caatccggta aggtggaggg aatctaagat gatgattgac ccgcgcacac
     9121 ctgaggggcg catgactctc cgttaccgtg gctaccggac tgaagtcctc ctcagagagc
     9181 ttggtctaga cccggaagat gagacccgcc agcaccaatc ccgagacgag ctaatagccc
     9241 aactggtcgc tatgaaactg ccgcttaacc gttagtagct cacctactca atacctaatt
     9301 taaacacctt ttgagatgac cgatgcgggg agacccgtag gccttactgt g
//
LOCUS       DAZSNM010000163         9344 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZSNM010000163 DAZSNM010000000
VERSION     DAZSNM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9344
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.163"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..2156
                     /locus_tag="ACHW42_003167"
     CDS             <1..2156
                     /locus_tag="ACHW42_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR8944875.1"
                     /translation="TLEYLNCITNIINACGVYPQDLKDREIISTFHAEKVINDLLKND
                     YKISLSPDTTYRELNKAAQSSITAPDRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEH
                     FTHIISDKPSPKDKYVAYAINIPDYELAADVYNINVTSPSGQQETFKILINPEHLRQT
                     LERKSLTAVQKSQCEIITPKKPGEAILHAFNATYQQIRENMSEFARSHYGYIQIPPVT
                     TFRADGPETPEEEKGYWFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYT
                     FNFMGIGGSGKNDLDYPSADIRAKISKDIESGIPGYWQGQVLIPFIKDPVTKERKQIH
                     PEDIHLTAKDFEASKDNISKDEWKNLHALKEKSLNGMPKTTPKSDQVIMLQNQYVREM
                     RKYGVRGLRYDAAKHSKHEQIERSITPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYL
                     VTCQLNEEQMSSLLYERDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSE
                     RRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTE
                     IKNYDGPTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSR
                     GDSVNINHSPHDGLVIINKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPP
                     CKVFTLRGGSLNINIPRRSALLLGKTGEPPNYLYL"
     gene            2492..3424
                     /gene="glaH"
                     /locus_tag="ACHW42_003168"
     CDS             2492..3424
                     /gene="glaH"
                     /locus_tag="ACHW42_003168"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR8944876.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3444..4712
                     /gene="lhgO"
                     /locus_tag="ACHW42_003169"
     CDS             3444..4712
                     /gene="lhgO"
                     /locus_tag="ACHW42_003169"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR8944877.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4735..6183
                     /gene="gabD"
                     /locus_tag="ACHW42_003170"
                     /pseudo
     CDS             4735..6183
                     /gene="gabD"
                     /locus_tag="ACHW42_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            6191..7477
                     /gene="gabT"
                     /locus_tag="ACHW42_003171"
                     /pseudo
     CDS             6191..7477
                     /gene="gabT"
                     /locus_tag="ACHW42_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            7715..9115
                     /gene="gabP"
                     /locus_tag="ACHW42_003172"
     CDS             7715..9115
                     /gene="gabP"
                     /locus_tag="ACHW42_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR8944878.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9136..>9344
                     /locus_tag="ACHW42_003173"
     CDS             9136..>9344
                     /locus_tag="ACHW42_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR8944879.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMS"
BASE COUNT         2367 a         2315 c         2420 g         2242 t
ORIGIN      
        1 atacattaga atatctaaac tgtataacaa atattattaa tgcttgtgga gtttatcctc
       61 aggatttaaa agacagagaa attatatcaa cttttcacgc agaaaaagtc attaatgatc
      121 tgttaaaaaa cgattataaa atttcccttt ctccagatac aacttaccga gagctgaata
      181 aagctgcaca gagtagcatt acagcgccag acaggatagg agaagggaaa acatgggttt
      241 atcaacgaga tacaatggtt gaaagaggtg ataacagtgg tgttcatcag tatggtccag
      301 ctgaacattt cactcacatt atatctgaca aaccgtcccc aaaagataag tatgttgcat
      361 atgctattaa cattcctgac tatgagctag cagccgatgt atataatatt aacgtgacgt
      421 caccttccgg acagcaagaa acatttaaaa tactaatcaa tccagaacat ctacggcaaa
      481 cacttgagcg taaatctctt actgctgttc agaaatcaca atgtgaaatc atcaccccca
      541 aaaaacctgg cgaagcgatt cttcatgctt ttaatgccac ctaccagcaa atcagggaaa
      601 atatgtctga gtttgcacgt tcccattatg ggtatataca aatccctcca gtgacaactt
      661 tccgcgccga cggaccagaa actcccgaag aagaaaaagg ttactggttt cacgcttatc
      721 aacccgaaga tctttgtacc atccataatc caatggggga tttgcaggat tttatcgcat
      781 tggttaaaga tgctaaaaaa tttggtatcg atatcattcc tgattatacc tttaacttta
      841 tgggaatcgg gggaagtggt aaaaatgacc tggattatcc ctctgctgat atacgagcga
      901 agatcagtaa agatatagaa agtggtatcc ctggctattg gcaaggtcag gttttgattc
      961 cattcattaa agatccagta acaaaagaac gtaaacaaat ccatccagaa gatatacatc
     1021 tcactgcaaa agacttcgaa gcaagtaaag ataacatctc taaggatgaa tggaaaaacc
     1081 tccatgcatt aaaagaaaag agtttaaatg gaatgcctaa aacaaccccc aaaagtgacc
     1141 aggttattat gttgcaaaat caatacgttc gtgaaatgcg aaaatatggc gtacgaggtt
     1201 tacgttatga tgcagcaaaa cactcaaaac atgaacaaat agaaagatca ataaccccac
     1261 cgcttaaaaa ttataatgag cgattacaca atactaactt atttaaccca aaatatcatg
     1321 aaaaaaccgt tatgaattac atggaatatc tggtaacttg tcagttgaat gaagaacaaa
     1381 tgtcatcgct gctttatgaa agagatgatt taagcgccat tgatttttca ttgctcatga
     1441 agacgataaa agccttttca ttcgggggag atctcctaac ccttgcatca aaaccgggtt
     1501 ccacaatctc aagcatccca tcagaaagac ggatattgat taacattaac cacgattttc
     1561 ctaacaatgg caatcttttc aatgactttc tatttaacca tcaacaagat gaacaattag
     1621 caatggcata tatggccgct ctcccgttca gcaggccttt agtttactgg gatggccagg
     1681 tattaaaatc aacgactgaa attaaaaatt atgatgggcc cacgcgtgtc ggtggtgagg
     1741 cgtggcttaa taaaggttgc tctacctatc agcagctcta caatgaattc cacgcattat
     1801 atatagataa agcaggaata tggagcgcat ttgagggtgt atttgcaact aaaaacgttc
     1861 tggcctttag tcgtggggat tctgtgaaca ttaatcactc tcctcatgat ggactagtta
     1921 taataaataa aggaaacgaa gaagttgaag gtacctggcc taacaaattg caacctggaa
     1981 tatataaaaa catggggagt aatagcgtta acattattat taataatacc cgaaaaatta
     2041 tccccccttg taaagtattt acgcttagag gcggatctct aaatatcaat attcctaggc
     2101 gtagcgctct tcttttaggg aaaacaggag aaccgccgaa ctatctctat ttataattta
     2161 atgttatatc tgccccgata aaacggggca gataatatgt ttagtttact aacggtcatt
     2221 ttgtagtgaa gccatttact gttttttatc gaccagataa tctgttctct aatgttaact
     2281 ccccctaacc tgttgcttta gttattcatt tcctgtctca ctttgcctta ataccctacg
     2341 ttaaatgtta ctaatttgtt gcttttgatc acaataagaa agcaatatgt cgcttttgtg
     2401 cgcatttttc agaaatgtag atatttttag attatggcta cgaaatgagc atcgccatgt
     2461 caccctacat ctcataagag gatcgcttct gatgaatgca ctgaccgccg tacaaaataa
     2521 cgctgtcgat tcaggccagg actatagcgg attcactctc atcccgtcgg cgcaatcccc
     2581 gcgtctgctg gaactcacct tcaccgaaca gacgaccaaa cagtttctcg aacaggttgc
     2641 cgaatggccc gtgcaggcgc tggagtacaa atcgtttctg cgttttcggg taggcaaaat
     2701 tcttgacgat ctgtgtgcga atcagctgca accattgctg ttgaagaccc tgctaaaccg
     2761 tgctgaaggt gcgctgttga tcaatgcggt aggtatcgat gatgtggcgc aggcagatga
     2821 gatggtgaag ctggcaacgg cggtagcgca tctgattggt cgctcaaatt tcgatgcgat
     2881 gagcggtcag tattacgcgc gtttcgtggt gaaaaatgtc gataactcag acagctatct
     2941 gcgtcagccg caccgcgtga tggaactgca caacgacggc acgtatgtcg aagagatcac
     3001 cgattacgtg ctgatgatga aaatcgatga ctgggaacat ctggaccact atttccgcca
     3061 tccactggcg cgtcgcccga tgcgctttgc cgcgccgccg agcaaaaacg tcagcaaaga
     3121 tgttttccat ccagtgttcg acgttgatca acagggtcgc ccggtgatgc gctatatcga
     3181 ccagttcgtc cagccaaaag attttgaaga aggcgtgtgg ttgagcgagc tttccgatgc
     3241 cattgaaacc agcaaaggca ttctttctgt acccgttccc gttggcaaat tcctgttgat
     3301 aaacaacctg ttctggctgc acggtcgcga ccgctttact ccgcacccgg atctgcgccg
     3361 tgaactgatg cgtcagcgtg gctatttcgc ttacgccact caccactacc agacgcatca
     3421 gtaagcgcaa aggaattgag cggatgtatg attttgtgat tattggcggc ggcatcatcg
     3481 gcatgtcgac cgccatgcaa ctgattgatg tttatccgga tgcccgcatt gcgttgctgg
     3541 aaaaagagtc cggcccggcc tgtcaccaga cgggccacaa cagcggcgtg atccatgccg
     3601 gggtctatta cacgcccggc agcctgaagg cgcagttttg cctggcgggg aaccgtgcca
     3661 ccaaagcctt ttgcgatcaa aacggcattc gctatgacaa ctgcggcaag atgctggtcg
     3721 ccacgtctca gctcgaaatg gaacggatgc gcgccttatg ggaacgcaca gcggcgaacg
     3781 gtatcgagcg cgagtggata aacgccgtgg aactgcgcga gcgcgaaccg aatatcaccg
     3841 ggcttggtgg catttttgtg ccgtccagcg gcattgtcag ctaccgcgaa gtgacggcgg
     3901 caatggcaaa aatcttccag gccagaggcg gcgagattat ttataacgcc gaagtcagcg
     3961 ccctcagtga gcataaaaac ggcgtggtga tacgcacccg tcagggcggt gaatatgaag
     4021 catcgacgct gattagctgt tccgggctga tggctgaccg gctggtgaaa atgctcggcc
     4081 tcgaaccggg ctttattatc tgcccgttcc gtggcgagta tttccgcctt gcgccggagc
     4141 ataaccagat tgttaaccac ctgatttacc ccattcccga ccccgcgatg ccatttttgg
     4201 gcgtgcatct cacccgcatg atcgacggca gcgtgaccgt cgggccaaac gcggtgctgg
     4261 ctttcaaacg cgaaggctat cgcaagcgcg acttctcatt tagtgacacg ctggagattt
     4321 tgagctcgtc ggggattcgc cgggtgctgc aaaaccatct acgctcagga ctgggcgaga
     4381 tgaaaaactc gctgtgcaaa agcggctatc tgcggctggt gcaaaagtat tgtccccggc
     4441 tttcgttaag cgatctccag ccctggcccg ccggtgtgcg ggcgcaggca gtatcgccgg
     4501 acggcaagct gattgacgat tttctgtttg tcaccacccc acgcacgatc cacacctgca
     4561 atgcgccctc cccggcagcg acatcagcaa ttcctattgg tgcgcatatt gtcagcaagg
     4621 tacaaacgct gttggcaagc cagagtaacc ccggacgcac gctgcgagcg gcacgtagtg
     4681 tggatgcctt acacgccgca tttaatcaat aacctttgaa aacaggacgt aacaatgaaa
     4741 cttaacgaca gtaacttatt ccgccagcag gcgttgatta acggggaatg gctggacgcc
     4801 aacaatggtg aagtcatcga cgtcaccaat ccggcgaacg gcgacaagct gggtagcgtg
     4861 ccgaaaatgg gcgcagatga aacccgcgcc gctatcgacg ccgccaaccg cgccctgccc
     4921 gcctggcgtg cgctcaccgc caaagaacgc gccaccattc tgcgcaactg gttcaatttg
     4981 atgatgcagc atcaggacga tttagcgcgc ctgatgaccc tcgaacaggg taaaccgctg
     5041 gctgaagcga aaggcgaaat cagctacgcc gcctccttca ttgagtggtt tgctgaagaa
     5101 ggcaaacgta tttatggcga caccattcct ggtcatcagg ccgataagcg cctgattgtt
     5161 atcaagcagc cgattggcgt caccgccgcc atcacgccgt ggaactttcc ggcggcgatg
     5221 attacccgta aagccggtcc ggcgctggcg gcaggctgca ccatggtgct gaagcccgcc
     5281 agtcagacgc cgttctctgc gctggcgctg gcggaactgg cgattcgcgc gggcattccg
     5341 gctggcgtgt ttaacgtggt caccggttcg gcaggcgtgg tcggtaatga actgaccagc
     5401 aacccgctgg tacgcaaact gtcgtttacc ggttagaccg agattggccg ccagttaatg
     5461 gaacagtgcg cgaaagacat caaaaaagtg tcgctggagc tgggcggcaa cgcgccgttt
     5521 atcgtctttg acgatgccga tctcgataaa gccgtggaag gcgcgctggc ctcgaaattc
     5581 cgcaacgccg ggcaaacctg cgtctgcgcc aaccgtttat acgtgcagga cggcgtgtat
     5641 gaccgctttg ccgaaaaatt acagcaggcg gggagcaaac tgcacatcgg cgacgggctg
     5701 gagaaaggcg tcaccatcgg gccgctgatc gatgaaaaag cggtagcaaa agtggaagag
     5761 catattgccg atgcgctgga gaaaggtgcg cgtgtggttt gcggcggtaa agcgcacgaa
     5821 cgcggcggca acttcttcca gccaaccatt ctggtggacg ttccggccaa cgccaaagtg
     5881 tcgaaagaag agacgttcgg ccccctcgcc ccactgttcc gttttaaaga tgaagccgat
     5941 gtgattgcac aagccaatga caccgagttt ggtcttgccg cctatttcta cgcccgtgat
     6001 ttaagccgcg tcttccgcgt cggcgaggcg ctggagtacg gcatcgtcgg catcaatacc
     6061 ggcattattt ccaatgaagt ggccccattc ggcggcatca aagcctcggg tctgggtcgt
     6121 gaaggttcga agtatggcat cgaagattac ttagaaatca aatatatgtg catcggtctt
     6181 taactggaga atgcgaagga acagcaataa agagttaatg cagcgccgca gtcaggcgat
     6241 tccccgtggc gttgggcaaa ttcacccgat tttcgctgac cgcgcggaaa actgccgggt
     6301 gtgggacgtt taaggccgtg agtatcttga tttcgcgggc gggattgcgg tgctcaatac
     6361 cgggcacctg catccgaagg tggtggccgc ggtggaagcg cagttgaaaa aactgtcgca
     6421 cacctgcttc caggtgctgg cttacgagcc gtatctggag ctgtgcgaga ttatgaatca
     6481 gaaggtgccg ggcgatttcg ccaagaaaac gctgctggtt acgaccggtt ccgaagcggt
     6541 ggaaaacgcg gtaaaaatcg cccgcgccgc caccaaacgt agcggcacca tcgcttttag
     6601 cggcgagtat cacgggcgca cgcattacac gctggcgctg accggcaagg tgaatccgta
     6661 ctctgcgggc atggggctga tgccgggtca tgtttatcgc gcgctttatc cttgcccgct
     6721 gcacggcata agcgaggatg acgctatcgc cagcatccac cggatcttca aaaatgatgc
     6781 cgcgccggaa gatatcgccg ccatcatgat tgagccggtt cagggcgaag gcggtttcta
     6841 cgccgcgtcg ccagccttta tgcagcgttt acgcgctctg tgtgacgagc acgggatcat
     6901 gctgattgcc gatgaagtgc agagcggcgc ggggcgtacc ggcacgctgt ttgcgatgga
     6961 gcagatgggc gtggcgccgg atcttaccac ctttgcgaaa tcgatcgcag gcggtttccc
     7021 gctggcgggc gtcaccgggc gcgcggaagt gatggacgct gttgcaccgg gcggtctggg
     7081 cggcacctat gccggtaacc cgattgcctg cgtggctgcg ctggaagtgt tgaaggtgtt
     7141 cgagcaggaa aatctgctgc aaaaagccaa cgatctgggg cagaagctaa aagacggatt
     7201 gttggcgata gccgaaaaac acccggagat cggcgacgta cgcgggctgg gatcgatgat
     7261 tgccatcgag ctgtttgaag atggcgatca cagcaaaccg gacgcaaaac tcaccgccga
     7321 gatcgtggca cgcgcccgcg ataagggcct gattcttctc tcctgcggcc cgtattacaa
     7381 cgtgctgcgc atccttgtac cactcaccat tgaagacgct cagatccgtc agggtctgga
     7441 gattatcggt cagtgttttg atgaggcgaa gcagtagcac cgtccccatg caggaggcga
     7501 cgctgcgcgt cttgtccggc ctacgagaat ccaagtcgga taaggcgttt acgccgcatc
     7561 cgacaatctg tacgtgaaca ggaataaatc tatgtcggcc ggataaggcg gagccgctct
     7621 ccggcaaaaa gaatcaataa caattaaacg cgtgacccga cgcgggaaat gtcggggcgc
     7681 tctcccaagt gacacacttt cgagaggatt caggatgggg caatcatcgc aaccacacga
     7741 gttaggcggc gggctgaaat cacgccacgt caccatgttg tctattgccg gtgttatcgg
     7801 cgcaagtctg tttgtcggtt ccagcgtcgc catcgccgaa gcggacccgg cggtattact
     7861 ggcctatctg ttcgccggat tactggtggt tatgattatg cggatgctgg cggaaatggc
     7921 agttgccacg cccgataccg gttcgttttc cacctatgcc gataaagcca ttggccgctg
     7981 ggcgggctat accatcggct ggttgtactg gtggttttgg gtactggtta tcccactgga
     8041 agccaacatc gccgccatga tcctgcactc gtgggttcca ggcattccca tctggttatt
     8101 ttccctcgtc attaccctcg ccttaactgg cagtaattta ttaagcgtta aaaactacgg
     8161 cgaatttgag ttctggctgg cgctgtgcaa agtcatcgct atcctggcct ttattttcct
     8221 tggtgcagtc gcaattagcg gtttttaccc ttatgccgaa gtgagcggga tctcaagatt
     8281 gtgggatagc ggcggcttta tgcccaacgg tttcggtgcg gtattaagcg cgatgttgat
     8341 caccatgttc tcgtttatgg gcgcagaaat tgtcaccatt gccgccgcgg aatccgacat
     8401 gccggaaaaa catattgtcc gcgccaccaa ctcggttatc tggcgtattt ctatcttcta
     8461 tttgtgctct atttttgtcg tcgtggcatt aataccgtgg aatatgccgg ggctgaaagc
     8521 cgttggatct tatcgctcgg ttctggaatt gctcaatatt ccccatgcga aattaatcat
     8581 ggactgtgtg atattacttt ccgtaaccag ttgcctgaac tcggccctgt ataccgcctc
     8641 aaggatgctc tactccttaa gccgtcgcgg tgatgctccc gcggtaatgg gtaaaatcaa
     8701 ccgcagtaaa accccgtacg tggcggtgtt actctccacc ggcgcagcat tcttaacggt
     8761 ggtggtgaac tattacgcgc ctgcgaaggt atttaaattt ctgatcgaca gctccggcgc
     8821 tatcgccctg ctggtttatt tagtcatcgc cgtttcacag ttgcggatgc gcaaaattct
     8881 gcgagcagaa ggaagcgaaa ttcgcttgcg aatgtggctt gatccctggc tcacctggct
     8941 ggtcatcggc tttattacct ttgtgttggt agtgatgcta ttccgtccgg cgcaacagtt
     9001 agaagtgatc tccaccggct tattagcgat agggattatc tgtaccgtgc caattatggc
     9061 tcgctggaaa aagctggtat tgtggcaaaa aacacccgtt cataatacgc gctgataatg
     9121 atcaggagtc acaccatgac cattacgtct ctggatggct atcgctggct gaagaacgat
     9181 attattcgcg gtaattttca accggatgaa aaattacgga tgagtttact gacatcgcgt
     9241 tatgcacttg gcgttgggcc gttgcaggaa gcactttctc aactggtggc ggaacggctg
     9301 gtcacggtgg tgaatcaaaa agggtatcgg gtggcgtcta tgtc
//
LOCUS       DAZSNM010000164         9328 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZSNM010000164 DAZSNM010000000
VERSION     DAZSNM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.164"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            77..1420
                     /locus_tag="ACHW42_003174"
     CDS             77..1420
                     /locus_tag="ACHW42_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR8944880.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            1645..3300
                     /gene="opgD"
                     /locus_tag="ACHW42_003175"
                     /gene_synonym="mdoD"
     CDS             1645..3300
                     /gene="opgD"
                     /locus_tag="ACHW42_003175"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR8944881.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            3440..3664
                     /locus_tag="ACHW42_003176"
     CDS             3440..3664
                     /locus_tag="ACHW42_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR8944882.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            3727..4263
                     /gene="rimL"
                     /locus_tag="ACHW42_003177"
     CDS             3727..4263
                     /gene="rimL"
                     /locus_tag="ACHW42_003177"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR8944883.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4258..5238)
                     /gene="ydcK"
                     /locus_tag="ACHW42_003178"
     CDS             complement(4258..5238)
                     /gene="ydcK"
                     /locus_tag="ACHW42_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR8944884.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            5362..6354
                     /gene="tehA"
                     /locus_tag="ACHW42_003179"
     CDS             5362..6354
                     /gene="tehA"
                     /locus_tag="ACHW42_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR8944885.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            6351..6944
                     /gene="tehB"
                     /locus_tag="ACHW42_003180"
     CDS             6351..6944
                     /gene="tehB"
                     /locus_tag="ACHW42_003180"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR8944886.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            7247..7903
                     /locus_tag="ACHW42_003181"
     CDS             7247..7903
                     /locus_tag="ACHW42_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFR8944887.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(7921..8427)
                     /gene="tnpA"
                     /locus_tag="ACHW42_003182"
                     /pseudo
     CDS             complement(7921..8427)
                     /gene="tnpA"
                     /locus_tag="ACHW42_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            8435..9328
                     /locus_tag="ACHW42_003183"
                     /pseudo
     CDS             8435..9328
                     /locus_tag="ACHW42_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
BASE COUNT         2410 a         2196 c         2321 g         2401 t
ORIGIN      
        1 cagtttgtaa attcttcccg ccagagtgaa tgcggttacc tacactccag attactgacc
       61 actggaggca gacactatgg cgaacagcat cacggcggat gagattcggg aacagttttc
      121 gcaggcaatg tcagccatgt accagcaaga agttccgcaa tatggcacgc tgctggaact
      181 ggtagctgat gtgaatctgg ctgtgctgga aaacaatcct caactgcacg aaaaaatggt
      241 aaatgcagac gagctggcgc gactgaatgt tgaacgtcat ggggcgattc gcgttgggac
      301 tgcacaagag cttgctactc ttcggcggat gtttgccatt atggggatgt acccggtgag
      361 ctattacgat ctctcgcagg caggggtgcc ggtacattcg acagcatttc ggcccattga
      421 tgatgcttct ctggcgcgta atcccttccg cgtttttaca tccttactcc gccttgagct
      481 tatcgagaac gaaattttgc gccagaaagc ggcggagatt ctgcgtcagc gcgatatctt
      541 caccccacgt tgtcgacaac tgttagagga atatgagcag cagggcggtt ttaacgaaac
      601 acaggcacag gagtttgtgc aggaagccct ggaaacgttt cgctggcacc agttagcaac
      661 ggtagatgaa gaaacctatc gcgccttgca caacgaacat cggttgattg ctgatgtggt
      721 ctgttttcct ggatgccata tcaaccacct gacgccacgt acgctggata ttgaccgggt
      781 gcagtcgatg atgcctgaat gcggaattga acccaaaatt ctcatcgagg ggccgccgcg
      841 ccgcgaggta ccgattttac tacgccagac cagctttaaa gcactggaag agacggtgtt
      901 gtttgcgggg cagaaacagg gcacgcatac cgcgcgcttt ggtgaaattg agcagcgtgg
      961 cgtggcatta acgccgaaag ggcgacaact gtatgatgat cttctgcgta acgctggaac
     1021 cgggcaggat aatctcactc accaaatgca tttacaggaa accttccgca cttttcctga
     1081 cagtgagttt ttaatgcgtc agcaagggtt ggcatggttc cggtaccgtc tgacgccttc
     1141 gggtgaggcg catcgtcagg cgattcatcc tggagacgat ccacagccct taattgaacg
     1201 tggttgggta gtggcgcaac ccatcaccta tgaagatttc ttgcccgtta gcgcggcggg
     1261 gatcttccag tcaaatctgg gtaatgaaac gcagacacgc agtcacggta atgccagtcg
     1321 cgaagcattt gagcaggcgt tgggttgtcc ggttttggat gagttccagc tttaccagga
     1381 agcggaagaa cgcagtaaac gtcgctgtgg tttgctttaa attctgacca tccgcctttg
     1441 caaaaatttg cctgatttta caaacgaatc aggctcatcc catcgacata aaaaaaatgc
     1501 cgatttatgc atattctctc agttcaacaa ttggattatt aataaatatt gtctagagtg
     1561 agcggtcata aataagcact ttcttgccgc tgaaaacgac cagcgcggga ccattcacaa
     1621 caccagaagg actcactttc aggtatggat cgtagacgat ttattaaagg ttcaatggct
     1681 atggccgccg tgtgcggtac cagcggcatt gcttctcttt tttctcaggc agcattcgcg
     1741 gcagattctg atattgccga cgggcaaacc cagcgttttg acttctccat tctacagtca
     1801 atggcgcacg acttagcgca aacagcgtgg cgtggtgcgc ctcgtccgtt acctgacacg
     1861 ctggcgacaa tgacgccgca ggcttataac agtattcaat acgacgccga aaaatcgctc
     1921 tggcataacg ttgagaaccg tcaactggac gctcagttct tccatatggg aatgggattc
     1981 cgtcgccgcg ttcgtatgtt ttctgtagat ccagcaacac atctggcgcg tgaaattcac
     2041 tttcgcccgg agttgttcaa atacaacgat gcaggtgttg ataccaaaca attagaaggg
     2101 caaagcgatc tcggctttgc cggttttcgc gtgtttaaag cccccgaact ggcgcgccgt
     2161 gatgtagtat catttctcgg cgcgagttat ttccgcgccg ttgatgatac atatcaatac
     2221 ggtttgtcgg cccgcggcct ggcgatcgac acttacaccg acagtaaaga agagttcccc
     2281 gactttaccg ccttctggtt tgatacggta aaaccggggg caactacctt taccgtttat
     2341 gcgttgctcg atagcgccag cattactggt gcctataagt tcactatcca ttgtgagaaa
     2401 agtcaggtga ttatggatgt ggaaaatcac ctgtatgcgc gcaaagacat taaacagctg
     2461 ggcatttcgc cgatgaccag tatgttcagc tgcggtacta atgaacgtcg gatgtgcgat
     2521 acaattcatc cgcaaattca tgactctgat cgtctgtcca tgtggcgggg caacggcgag
     2581 tggatttgcc gtccgctgaa taatccgcaa aaattgcagt tcaatgctta caccgacaac
     2641 aacccgaaag ggtttggttt attgcaactg gatcgtgact tctcccatta tcaggacatt
     2701 atgggctggt ataacaaacg cccaagtctg tgggtggaac cgcgtaacaa gtggggtaag
     2761 ggcaccatcg gcctgatgga aatcccaaca acgggcgaaa cgctggataa cattgtctgc
     2821 ttctggcagc cagaaaaagc tgtaaaagca ggtgatgagt ttgcattcca gtatcgtctg
     2881 tactggagtg cgcaaccgcc tgttcattgc ccattagcgc gcgttatggc gacgcgtacc
     2941 ggcatgggcg gtttcccgga aggttgggcg ccaggtgaac actatcccga aaaatgggcg
     3001 cgtcgttttg ccgtcgattt cgttggtggt gatctgaaag ctgccgcgcc aaaaggcatt
     3061 gagccggtga ttacgctttc cagtggggaa gcgaagcaaa tcgaaattct ctatattgaa
     3121 cccatcgatg gttatcgtat tcagtttgac tggtatccga cttcggactc cactgatccg
     3181 gtcgatatgc ggatgtatct acgttgtcag ggggacgcta tcagtgaaac atggctgtat
     3241 cagtatttcc cgccagcgcc ggataaacgt cagtatgttg acgaccgcgt gatgagttaa
     3301 tcgttttttc ttcggcacct tcttcgggag gtgccgtctg gttaaacacg atcccgctcg
     3361 catttttccc taagttaaat gagtaatctg atggtgtgta tttcagatac accgtgtcag
     3421 ccactaacag ggagtgcgta tgtttccaga ataccgagat ttaatatccc gtctgaaaaa
     3481 cgaaaatcct cgctttatgt ccttgttcga taaacacaat aaacttgatc atgaaattgc
     3541 cagaaaggaa ggttccgacg gtcgagggta caatgcggaa gtggtccgca tgaaaaaaca
     3601 aaagctacag ttaaaagatg agatgctcaa aatcctgcag caggagagcg tcaaagaggt
     3661 gtaaactttc ctaagccgcc agccaggcgg cttttttaac aacagcatgg attgactgga
     3721 gataagatga ctgaaacgat aaaagtaagc gaatcacttg aattacatgc tgttgcggaa
     3781 agtcacgtca cacctcttta tcagttaatc tgtaaaaata aaacctggtt acagcagtcg
     3841 ctaaactggc cgcagttcgt tcagagtgaa gaggacacgc gaaaaacggt gcagggtaat
     3901 gtgatgttgc atcaacgcgg ctatgccaaa atgttcatga ttttcaaaga agatgaactt
     3961 atcggcgtta tctcgtttaa tcgtattgaa ccactgaata aaactgctga aatcggctac
     4021 tggctggacg aatctcatca ggggcagggg atcatttctc aggcgctgca gacattgatt
     4081 catcattacg ctcagtctgg tgaacttaga cgcttcgtga tcaaatgtcg ggtggacaat
     4141 ccgcaaagca accaggtcgc tttgcgcaat ggttttatcc ttgaaggttg cctgaaacag
     4201 gctgagttcc tgaatgatgc ctatgatgat gtgaacttat acgcgcgtat tatcgattca
     4261 taataaccct gccagcggcg ttcgcgtaat gcgctcctcg ccgttaatga cttttggacc
     4321 acggacatga accgtgtcgc catcaaaggc ttcaacgacg gcgtggtcgg ttaactccac
     4381 atggttttca attatcaccg cacctgtaat acggctctca ccttgtatta caacgtgctc
     4441 gtcgagcaga atcggtccac cacgtaccac ggcatttccg ccaatcaaga catgatgttt
     4501 taacacacaa ttaccttcca caatggcata ttccgccacc tgcgaactgt aatgaatcgt
     4561 cggaatggca tcttcttcta tgccagcttt cacctgcgcg tgaccgtaga ctttagcgca
     4621 atcgcatagc cagacgttgt tctcttcatt accttcaaca ctggcaaaat caaaaacttc
     4681 ggcgcgatgt tcaataaaag cataccggac gacggcatcg ccataaattt gtgcctggtg
     4741 gacaatacgc gaggcgctaa cccttgcgcg atcataaatt tggagcagga gttgatggtc
     4801 gggcgttaag ccttgtgcgg cgacgatcat agaatgttga tcaattaagg cgtgaccaaa
     4861 tattctacat tgcacgtaaa ctaatgagtc atgaatggtg acactgtcac ttatatatgc
     4921 accctggctg atttcactat tatcgatcca gacgttatcc gttgcgtaaa cctctcccca
     4981 cagcacgctg gtgccggtga tacgcgtatt accggaaatc accgttccgc cgaacgcaat
     5041 agcattctgg tcgtaaatcc agcaatttcc ttcctgtgct aacaccgttt cgcgatcgat
     5101 ccagccgcca gcggttcctg ctatcacatc gttaaaatcg ctaatggcga taatctgccg
     5161 taataacaca ctttttttag tgccatcttc ctgataacta aaggctcgct gttcttcact
     5221 taggcgatat ttgcgcatag cgttttccca caggtgactt actataaccg tagcaaattc
     5281 tgcggctctg gctatgctcg agaaaattcc ataaaatgca tttcaaatat actttataaa
     5341 ttaaacaaaa tgagtaagaa gatgcagagc gataaagtgc tcaatttgcc ggcaggctac
     5401 tttggtattg tgttggggac gatagggatg ggatttgcct ggcgctatgc cagccaggtt
     5461 tggcaggtca gccactggtt aggggatggg ctggtgattc tggcgatgat catctgggga
     5521 ttattgacta gcgcatttat tacccgactc atacgctttc cgcatagcgt gctggcggaa
     5581 gttcgccatc cagtgctgag cagttttgtg agtttgtttc cggcaacgac gatgctggtg
     5641 gcgattggtt ttgttccgtg gtttcgccca ctggcggtgt gcatgttcag ttttggtgtc
     5701 gtggttcagt tggcttatgc cgcctggcaa actgcgggat tatggcgcgg atctcaccct
     5761 gaagaagcta ccacgcctgg actgtatctg ccgacagttg ccaacaactt tatcagcgca
     5821 atggcctgtg gtgcgttggg ctacaccgac gccggtctgg tgtttttagg cgcaggcgtt
     5881 ttctcatggc taagccttga accggtgatc ttgcagcgtc tgcgcagttc gggagaatta
     5941 cccacggcac tgcggacatc actcggcatt cagctcgctc ctgcgctggt ggcctgtagt
     6001 gcctggctga gcgtcaacgg cggcgagggt gacacgctgg cgaaaatgct tttcggttat
     6061 ggactgctgc aactgctgtt tatgctacgt ctgatgccat ggtatctctc ccagccattt
     6121 aatgcttcat tctggagttt ctcgttcggc gtatctgcac tggcaaccac cggtttgcat
     6181 ctggggagtg gcagcgataa tggatttttc catacgctgg cggtgccgct gtttatcttt
     6241 accaatttta ttattgcaat actgctcatc cgtacttttg cgcttctgat gcagggaaaa
     6301 ttgttagtca gaaccgagcg cgccgtttta atgaaagcag aggacaaaga atgatcattc
     6361 gtgacgaaaa ctattttact gataaatatg aattaacccg cacacactct gaagtactgg
     6421 aagcggtgaa agtggttaaa ccgggtaaaa cgctggatct gggctgtggc aatggtcgta
     6481 acagtcttta cctggcagcc aatggttatg atgttgacgc atgggataaa aatgccatga
     6541 gtatcgccaa cgtcgagcgc attaaatcca ttgaaaatct ggataattta cacacccggg
     6601 tcgttgatct gaataacctc acatttgatg gacagtacga ttttattctt tcgactgtgg
     6661 tgctgatgtt ccttgaggct aaaaccatcc cagggttgat tgccaatatg caacgttgca
     6721 ctaaacctgg tggttacaac ctgattgtgg cggcgatgga taccgctgat tatccatgta
     6781 ccgtcggctt cacgtttgcc ttcaaagagg gagaattacg tcgatattac gaaggctggg
     6841 agagggtgaa atacaatgaa gacgtcggcg agctgcaccg caccgacgcc aacggtaatc
     6901 gtattaaact gcgtttcgcc acgatgctgg cacgtaaaaa atgacccggt aagcacaaaa
     6961 cgcgtgaaaa ttccccacgc tgagatgatt tactgttctt cttttcggta agcatatttt
     7021 ttatcgaagg gatgtgaaat taatcacagt agtcgaagtt ttttagcagc ttaacttact
     7081 gaaatttaac tactgatgat tgacttagcc cctttttcgg cattgactat gtcgtctgaa
     7141 aaggggctga aaaatttatt ttcaccaaca ctttttttgc cacaacacga agcggcgctt
     7201 tttgctataa cttagaaagt aatataatca tctcaggaaa ctattcatgc gtaccacatc
     7261 atttgcgaaa gttgcagctt tatgcggctt gttggctctg tctggttgtg cctctaaaat
     7321 cacccagcca gataaatata acaattactc tgatttaaaa gaaacaacct cagcaacagg
     7381 taaacctgtt ttacgttggg tagacccgag ttttgatcaa agcaaatatg acagcatcgt
     7441 ctggaaccca atcacttatt atccggtacc aaaaccttct acccaggtag ggcagaaagt
     7501 tctggataaa attttgaact ataccaacac cgaaatgaaa gaagcgatag cgcagcgtaa
     7561 accagtggtt accaccgctg ggccgcgtag tctgattttc cgtggggcca ttaccggtgt
     7621 agataccagc aaagaagggc tgcaattcta tgaagtggtt cctgttgcat tagtggttgc
     7681 ggggacgcaa atggctacag gccaccgtac catggatact cgcctctatt ttgaaggtga
     7741 gctgattgat gcagcgacta ataaaccggt tatcaaagtc gttcgtcagg gcgaaggtaa
     7801 agacctgaat aacgaaagta cgccaatggc tttcgaaaat attaaacaag ttattgatga
     7861 catggcgacc gatgccacca tgtttgacgt taacaaaaag tagttccaga cgcgccatcg
     7921 ttagatggcg ctttttatcc ggtgcgccgt aaaaccccat ccttcagggc ggggatataa
     7981 ggcgcggttt tccacctgac caggtgtttg ctgttgttca atgtattggc ggatgacgga
     8041 tattggcgca cctccgcagc tactggcaaa atagccagga ctccacaaaa cgcctttgta
     8101 gtaatacctg actgcaatat ctggtcgatc tcgtcgcagt aacctacccg atacgccttt
     8161 gaggctgttt accagactgg atatcgccag tttgggaggt tagttaatta acaaatggac
     8221 gtgatctggt tcgccatcca tttcaaccag ttcagcttca aaataagcac atacatttga
     8281 aaagtaagtg cgtagttttt ctgtcgcgtc atggtcaaaa atctggcgtc ggtatctggt
     8341 gacaaagact aggtgaacat gcatcaggaa aacacaatgc cttccacgcc ggatatcggt
     8401 ttcttttttc acagaccaaa gtatcatttg acctgtgaaa cgattacagg catttaaatt
     8461 ccagttaaga ccaggtggtc aacaggagtg tgaaatgagg cgcttcgcag gcgcatgtcg
     8521 tttcgttttc aatcgtgctc tggcacgtca gaatgagaat cataaggccg gtaataaata
     8581 catcccttac gggaaaatgg cttcctggct ggttgagtgg aaaaatgcca ctgaaacgca
     8641 atggcttaaa gattctccct cacagccatt gcaacagtca ctgaaagacc ttgagcgggc
     8701 ttacaaaaac ttcttccgga agcgggctgc ttttccccga ttcaaaaagc ggtgacagaa
     8761 tgatgcattc cgctacccgc agggcgttaa gctcgatcag gaaaacagcc gtatttttct
     8821 gccgaaactg ggctggatgc gctaccggaa tagccgtcag gtcacgggtg ttgtgaaaaa
     8881 tgtcactgtc agccagtcct gcggtacgtg gtacatcagt attcagacag aaagtgaagt
     8941 atccactccg gctcaccctt cagcatcaat ggtcgggctg gatgctggcg tggctaaact
     9001 cgccacgctg tcagatggca cagtctttga gcctgtaaac agttttcaga aaaaccagaa
     9061 gacgctggcg agacttcagc gacagttaag ccgcaaggtc aaattcagca acaactggca
     9121 gaagcagaaa cgcaaaatac agcgactgca ttcctgtatc gcaaatatcc gcagggacta
     9181 ccttcacaaa gtcacaacgg ccgtcagcaa aaaccacgca atgatagtca ttgaggattt
     9241 gaaggtcagc aacatgtcaa agtcagcagc gggtacggtc agccagccgg ggcgcaatgt
     9301 ccgggcaaaa tcaggtttaa accgttca
//
LOCUS       DAZSNM010000165         9291 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZSNM010000165 DAZSNM010000000
VERSION     DAZSNM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.165"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="ACHW42_003184"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="ACHW42_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR8944888.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="ACHW42_003185"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="ACHW42_003185"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR8944889.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHW42_003186"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHW42_003186"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR8944890.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHW42_003187"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHW42_003187"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR8944891.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHW42_003188"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHW42_003188"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR8944892.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHW42_003189"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHW42_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR8944893.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6799)
                     /gene="nth"
                     /locus_tag="ACHW42_003190"
     CDS             complement(6164..6799)
                     /gene="nth"
                     /locus_tag="ACHW42_003190"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR8944894.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="ACHW42_003191"
     CDS             complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="ACHW42_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR8944895.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="ACHW42_003192"
     CDS             complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="ACHW42_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR8944896.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="ACHW42_003193"
     CDS             complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="ACHW42_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR8944897.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2384 a         2326 c         2357 g         2224 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg
     5821 cggcgatatg ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta
     6601 gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa
     6661 cactgatatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac
     6721 tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca
     6781 gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc
     6841 agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat
     6901 cagtcccaag ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt
     6961 gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc
     7021 aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt
     7081 tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc
     7141 gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc
     7201 taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac
     7261 cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag
     7321 cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag
     7381 agcgttagtg gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag
     7441 tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta
     7501 ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc
     7561 gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa
     7621 atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc
     7681 cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt
     7741 ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag
     7801 cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg
     7861 tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt
     7921 taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc
     7981 aaataacgtc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt
     8041 gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt
     8101 tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta
     8161 gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg
     8221 atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag
     8281 ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag
     8341 catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa
     8401 ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg
     8461 aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat ttacccattg
     8521 ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga
     8581 atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc
     8641 aatacctaag cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac
     8701 ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct
     8761 ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa
     8821 cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc
     8881 cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt
     8941 cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac
     9001 gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca
     9061 accaaaaaac cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat
     9121 aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat
     9181 ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc
     9241 cgcggcaact gcggctttgc gcggatcgac ctgttcttct ggttccgcat t
//
LOCUS       DAZSNM010000166         9242 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZSNM010000166 DAZSNM010000000
VERSION     DAZSNM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.166"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="ACHW42_003194"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="ACHW42_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR8944898.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="ACHW42_003195"
     CDS             1828..3147
                     /locus_tag="ACHW42_003195"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR8944899.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3524..4594
                     /gene="dosC"
                     /locus_tag="ACHW42_003196"
                     /pseudo
     CDS             3524..4594
                     /gene="dosC"
                     /locus_tag="ACHW42_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            4594..5211
                     /locus_tag="ACHW42_003197"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="ACHW42_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="ACHW42_003198"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="ACHW42_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /gene="ddpC"
                     /locus_tag="ACHW42_003199"
     CDS             5815..6711
                     /gene="ddpC"
                     /locus_tag="ACHW42_003199"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR8944900.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="ACHW42_003200"
     CDS             6708..7682
                     /locus_tag="ACHW42_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8944901.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="ACHW42_003201"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="ACHW42_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="ACHW42_003202"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="ACHW42_003202"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR8944902.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2259 a         2257 c         2317 g         2409 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
     9241 ag
//
LOCUS       DAZSNM010000167         9070 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZSNM010000167 DAZSNM010000000
VERSION     DAZSNM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.167"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..923
                     /locus_tag="ACHW42_003203"
     CDS             <1..923
                     /locus_tag="ACHW42_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8944903.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHW42_003204"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHW42_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR8944904.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHW42_003205"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHW42_003205"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR8944905.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="ACHW42_003206"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="ACHW42_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR8944906.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="ACHW42_003207"
     CDS             4446..4805
                     /locus_tag="ACHW42_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR8944907.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHW42_003208"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHW42_003208"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR8944908.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="ACHW42_003209"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="ACHW42_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR8944909.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="ACHW42_003210"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="ACHW42_003210"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR8944910.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2066 a         2196 c         2448 g         2360 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg
//
LOCUS       DAZSNM010000168         9012 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZSNM010000168 DAZSNM010000000
VERSION     DAZSNM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.168"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHW42_003211"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHW42_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR8944911.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHW42_003212"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHW42_003212"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR8944912.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHW42_003213"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHW42_003213"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR8944913.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHW42_003214"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHW42_003214"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR8944914.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="ACHW42_003215"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="ACHW42_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR8944915.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /gene="smg"
                     /locus_tag="ACHW42_003216"
     CDS             5545..6018
                     /gene="smg"
                     /locus_tag="ACHW42_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR8944916.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="ACHW42_003217"
     CDS             6047..6589
                     /locus_tag="ACHW42_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR8944917.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="ACHW42_003218"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="ACHW42_003218"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR8944918.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="ACHW42_003219"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="ACHW42_003219"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR8944919.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="ACHW42_003220"
     CDS             7986..8243
                     /locus_tag="ACHW42_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR8944920.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="ACHW42_003221"
     CDS             complement(8219..8773)
                     /locus_tag="ACHW42_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR8944921.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2254 a         2188 c         2249 g         2321 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatggcaggg
     4441 agataaggat ggtcgatata gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taataccatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatggta aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgctttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaga agatcaaaaa aatacttgtg caaaatattg
     8941 ggatccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag ag
//
LOCUS       DAZSNM010000169         8947 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZSNM010000169 DAZSNM010000000
VERSION     DAZSNM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8947
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.169"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="ACHW42_003222"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="ACHW42_003222"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR8944922.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="ACHW42_003223"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="ACHW42_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR8944923.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHW42_003224"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHW42_003224"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR8944924.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="ACHW42_003225"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="ACHW42_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR8944925.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="ACHW42_003226"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="ACHW42_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR8944926.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHW42_003227"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHW42_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR8944927.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHW42_003228"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHW42_003228"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR8944928.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..>8947
                     /locus_tag="ACHW42_003229"
     CDS             8747..>8947
                     /locus_tag="ACHW42_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFR8944929.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPA"
BASE COUNT         2273 a         2239 c         2248 g         2187 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttatgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcg
//
LOCUS       DAZSNM010000170         8896 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZSNM010000170 DAZSNM010000000
VERSION     DAZSNM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8896
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.170"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..398
                     /locus_tag="ACHW42_003230"
     CDS             <1..398
                     /locus_tag="ACHW42_003230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR8944930.1"
                     /translation="VVDRNLKPEGIFLLHTIGSKKTDLNVDPWINKYIFPNGCLPSVR
                     QIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPEIADNYSERFKRMFTYYLNA
                     CAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(438..1079)
                     /gene="ribE"
                     /locus_tag="ACHW42_003231"
     CDS             complement(438..1079)
                     /gene="ribE"
                     /locus_tag="ACHW42_003231"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR8944931.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            1294..2667
                     /gene="mdtK"
                     /locus_tag="ACHW42_003232"
     CDS             1294..2667
                     /gene="mdtK"
                     /locus_tag="ACHW42_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR8944932.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(2708..3964)
                     /locus_tag="ACHW42_003233"
     CDS             complement(2708..3964)
                     /locus_tag="ACHW42_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR8944933.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            4272..4348
                     /locus_tag="ACHW42_003234"
     tRNA            4272..4348
                     /locus_tag="ACHW42_003234"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4306..4308,aa:Val,seq:gac)
     gene            4353..4429
                     /locus_tag="ACHW42_003235"
     tRNA            4353..4429
                     /locus_tag="ACHW42_003235"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4387..4389,aa:Val,seq:gac)
     gene            4537..4842
                     /locus_tag="ACHW42_003236"
     CDS             4537..4842
                     /locus_tag="ACHW42_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR8944934.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            4968..6390
                     /locus_tag="ACHW42_003237"
                     /pseudo
     CDS             4968..6390
                     /locus_tag="ACHW42_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(6402..7214)
                     /gene="ydhT"
                     /locus_tag="ACHW42_003238"
     CDS             complement(6402..7214)
                     /gene="ydhT"
                     /locus_tag="ACHW42_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR8944935.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(7218..8003)
                     /gene="phsC"
                     /locus_tag="ACHW42_003239"
     CDS             complement(7218..8003)
                     /gene="phsC"
                     /locus_tag="ACHW42_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR8944936.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(8000..8668)
                     /locus_tag="ACHW42_003240"
     CDS             complement(8000..8668)
                     /locus_tag="ACHW42_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR8944937.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(8732..>8896)
                     /locus_tag="ACHW42_003241"
     CDS             complement(8732..>8896)
                     /locus_tag="ACHW42_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944938.1"
                     /translation="EAAIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCE
                     WFEVEQFQNP"
BASE COUNT         2164 a         2322 c         2076 g         2334 t
ORIGIN      
        1 cggtggtgga tcgtaatttg aaaccggaag gtatattcct gctccatact atcggttcga
       61 aaaaaaccga tctgaatgtt gatccctgga ttaataaata tatttttccg aacggttgcc
      121 tgccctctgt acgccagatt gctcaatcca gcgaacccca ctttgtgatg gaagactggc
      181 ataacttcgg tgctgattac gatactacgt tgatggcgtg gtatgaacga ttcctcgccg
      241 catggccaga aattgcggat aactacagtg aacgctttaa acggatgttt acctattatc
      301 tgaatgcctg cgcaggtgct ttccgcgccc gtgatattca gctctggcag gtggtgttct
      361 cacgcggtgt tgaaaacggc cttcgagtcg ctcgctaaag gctattctat cgccccctct
      421 tcgggggcga gttcagatca ggcttctgta cctggttgat tcatggcatt ttctcgtgcc
      481 gccagcacac gttctaccgt atctaccact gcctgagttt gtggatcgat ttcaatgttg
      541 acgcgtgcgc caagtttttt cttcccaaga gtcgtgcgtt ccagtgtttc cggaattaaa
      601 tggacgcaaa aacgcgttgg cgtgacttcg ccgacggtca ggctaatacc gtcgatgcca
      661 ataaatcctt tgtacaggat atatttcatc aactgactat cctggacttt aaaccagatc
      721 tggcgattat tttccgaggt taatattttc gccacttcag cggtggtcat aatatgacct
      781 gacattaagt gtccgccaat ttcatcactg aatttcgccg cacgctcaac gtttacccaa
      841 tcccccactt ttaaatcgcc aagattggta atgcgtaacg tttctttcat caggtcaaaa
      901 ctgacatggt tgccgttaat ttccgtcacg gtcaggcagc aaccgttatg cgccacggaa
      961 gcaccggttt ccaggccgtc cagcatgtgg tcgggtaact ccaccacatg cgtacgaaaa
     1021 tttggtttct cgtcaatcga caccagtttt gcggtgccct gtacaatccc cgtaaacata
     1081 cttacaactc ctgaaatcag ttaagacatt ctgttcagca caatagcagg tggaaaacgc
     1141 ccttaccagt gaaggggtaa gaatggctat tttttcactg gagaattaat aaatcctcgc
     1201 tacaatagac tgaatttccc ctgcttcttc tttttgctgc ccattcaggc ggctttttag
     1261 tctctcatat aactacaaat aaaaggtgtt cacgtgcaga agtatatcag tgaagcgcgt
     1321 ctgttattag cattagcaat cccggtgatt ctcgcgcaaa tcgcccaaac tgcgatgggt
     1381 tttgtcgata ccgtgatggc gggcggctat agtgccaccg acatggcggc ggtcgctatc
     1441 ggtacttcta tctggcttcc ggcgatcctc tttggtcacg gactgctgct ggcattaacg
     1501 ccggttatcg cgcaattaaa tggttccggt cgacgtgagc gcattgcgca tcaggtgcga
     1561 caaggtttct ggctggcagg ttttgtttcc gttctcatta tgctggtgct gtggaatgca
     1621 ggttacatta tccgctacat ggaaaacatc gatccggctc tggcggacaa agccgtgggt
     1681 tatctgcgtg cgttgttgtg gggcgcgccg ggatatctgt tcttccaggt tgcccgtaac
     1741 caatgtgaag gtctggcaaa aaccaagccg ggtatggtaa tgggctttat cggcctgctg
     1801 gtgaacatcc cggtgaacta tatctttatt tatggtcatt tcggtatgcc tgagctcggt
     1861 ggcgttggtt gtggcgtggc tactgcggcg gtgtattggg tcatgttcct tgccatggtt
     1921 tcttacatta aacgcgcccg ctccatgcgc gatattcgta acgaaaaagg caccgctaag
     1981 cccgagcctg cggttatgaa acgactgatt caactcggtt tgccgattgc gctggcactg
     2041 ttctttgaag tgacactgtt tgccgtcgtg gctctgttag tgtctccgct cggtattgtt
     2101 gatgtcgcag gacaccagat tgccctgaac tttagttcac tgatgtttgt gcttccaatg
     2161 tcgctggcgg cagcggtaac tatccgcgta ggttatcgtc tgggtcaggg ctcaacgctg
     2221 gatgcgcaaa ccgctgcgcg gaccgggctt atggtgggtg tctgtatggc aaccctgacg
     2281 gccattttca cggtttcact gcgggagcaa atcgccctgt tgtacaacga caatcccgag
     2341 gttgtaacgc tggctgcgca tttgatgttg ctggcggcgg tatatcagat ttctgactca
     2401 atccaggtga ttggcagtgg gattttgcgt ggttataaag atacgcgttc cattttctat
     2461 atcaccttta cagcttactg ggtgctgggc ttgccaagcg gctatattct ggcactgacc
     2521 gatctggtcg ttgaacctat ggggccagca ggcttctgga taggctttat tatcggcctg
     2581 acatcggcag ccattatgat gatgctgcgt atgcggttcc tgcaacgtat gccgtcagcc
     2641 atcattctgc aacgagcgtc ccgctaataa agacaaggcg caaccttcac gggttgcgcc
     2701 tgtgttttta cgcaggctgg agcgttgcgc caatcccgtc ttcgtctggc tgtaatttca
     2761 gagcgttaca cagagtttgc cgactggcat ctgttatctc aacgcgtata tccccgttaa
     2821 cttccccttt cacattgatg aagtcaggtg aaatttcttt cgcactggtt tgcaggcaaa
     2881 tcgctccctc tccgcgcagg cgtttcactt tcagttgcac atgcgttgcc gcaccagtga
     2941 tcagccacag ttttccgcca ttcagattga tgttaccaac aatgaaagtg acgctgtcac
     3001 cttttaccgt actgccgtcg agaatcaaca caccgccctg ctgtacagta atcgttgatt
     3061 gttgcagctc tgcgccataa ccaataatca ttgaagcgcc gtccagtaat gcaacactcc
     3121 cggtcagttc aaccggtgcg cgatcttcct ctacgacaaa attttcgtcc gcgctggtgg
     3181 gtgacaacag gatcactgtt ccaccttgta ttaccgcatt ttccagcgta ccggcatgca
     3241 cttcagcccg cccccccaca ttcaccgaca ggttttgcgt cttaccggaa ctgtagagct
     3301 gaaggccatc cgtccccaga cgataaatgg atccattctc aacaatagtg tcagagtctt
     3361 caccaccgag attattcatg gtgccaccag caagcaacgt atcactggct ttcccggcca
     3421 gcataaaaac gccgccctga ttaatctcac aattgctggc ttctccactt ggatacacca
     3481 acaattcacc accttcatct accacgaccg ctgaggttgt cccattaacc aacaggccgc
     3541 cctcttgatc gagaatgatt ttttccgcgc gatgtccttc cagtacacgc aggttaccgc
     3601 cattttccag caacaaaccg caggcataac cctgatcaac gctgaattca ccttcgggat
     3661 ggcggccatt aaccgtagcg agcgtagaga gactaattgc gcctccctcg gactgaacaa
     3721 tatctgtggc aatggcaccg tcgctgaccg atagcgtgcc attgttaccg tactgtgtgc
     3781 atttggcgat cccaccggtt gccactttca accagccagc ccccgtgacc acggtatttt
     3841 cggctcttcc gccagcttca acaatcaact cgccttcagt aagttgcgtc gcgccaagga
     3901 cctcgccggt cttaacaatt tgcacattcc cgtcgctcat caagtttttc gcatcagatc
     3961 ccatgattta ttcctttgct gcatctgtgt gcctttattg ctacctaagt gtaaaggcta
     4021 cggaggattt atccacgaca gatttgagat ggtggcaaac aactctgttt aaactctgat
     4081 acacgaatta ttgggttgta tcagatgtaa atgcgatcct gaataaaaat cgcccttgca
     4141 aatcaacaaa atatagccaa tttgcttaaa agctctgcaa acggtcgaaa tagcgcagaa
     4201 aattacgttt tgcctcttgc cacctttcca ctctgccgct aatattcgtc cccgttgtca
     4261 cctacaacgt tgcgttcata gctcagttgg ttagagcacc accttgacat ggtgggggtc
     4321 gttggttcga gtccaattga acgcaccatc ctgcgtccgt agctcagttg gttagagcac
     4381 caccttgaca tggtgggggt cggtggttcg agtccactcg gacgcaccag attttcttaa
     4441 tctggtcttt tcctttttcc ctctgtttct tctctgtatc caatacgtta aaagatttac
     4501 actgtcttcg tatgcgttat cagaaggaga atcgctatgg caactttgtt acaacttcat
     4561 tttgctttta atggcccatt tggcgacgca atggctgagc agcttaaacc acttgctgag
     4621 tcgattaatc aggaacctgg ttttctgtgg aaggtatgga cagaaagtga aaagaaccac
     4681 gaagccggtg ggatctacct tttcactgat gaaaaaagcg cccttgccta tctggaaaaa
     4741 catactgccc gactgaaaaa tctcggcgtt gaggaagttg tcgccaaagt tttcgatgtc
     4801 aatgagccac ttagtcaaat caatcaggca aaactcgcct gacagaattt aatcaagggc
     4861 ggttagcgcc cttttcatcc ctgtctgaaa tttctcaaat tctaaaaatc tcaaccaaac
     4921 ttatctgata acactaaatt cgaaagaatg cgtacaggta agtaacaatg aaaaaaattg
     4981 ctattgtggg tgccgggcct acggggatct acaccttatt ctcgcttcta cagcaacaaa
     5041 ctccactttc tatttctatc ttcgagcagg ctgacgaggc cggtgtcggg atgccataca
     5101 gtgatgagga aaactcaaaa atgatgctgg caaatattgc cagtattgaa ataccgccga
     5161 ttaattgtac gtatctcgaa tggctacaaa agcaagaagc cagccatctc cagcgttatg
     5221 gcgttaaaaa agaaaccttg cacgatcgtc agtttttacc gcgaattctg ctgggcgaat
     5281 atttccgcga tcaattttta cgattagtag accaggcacg acagcaaaaa tttgcagtgg
     5341 ctgtttatga atcatgccag gttaccgatc tgcaaattac aaatgctggc gtcatgctcg
     5401 ctacaaatca ggatttaccc agcgagacgt ttgatttagc ggtgattgcc acgggtcacg
     5461 tctggcctga tgaagaagaa gcaaccagaa cgtattttcc cagcccgtgg tcaggcctga
     5521 tggaagcaaa ggtcgatgcg tgtaacgtgg gtattatggg aacatccttg agcggactgg
     5581 atgcggcaat ggcagtggct attcagcatg gttcgttcat tgaagatgat aaacaacacg
     5641 tcgtttttca ccgcgataac gcaagtgaaa agctaaatat tacgttgatg tcgcgcacgg
     5701 gtattttacc cgaagccgat ttctattgcc ctattcccta cgagccctta cacatcgtca
     5761 ccgatcaggc attaaatgct gagattcaaa aaggtgaaga gggccttttg gatcgggtat
     5821 ttagattgat agtagaggaa atcaagtttg ctgatccaga ctggagccaa cgcatagcct
     5881 tagagagcct gaatgtcgat tcctttgctc aagcctggtt tgccgagcgc aaacaacgcg
     5941 acccatttga ctgggcagaa aaaaatctcc aggaagtcga acgcaataaa cgagaaaaac
     6001 atactgttcc ctggcgttat gtcattctgc gcctgcatga agccgtacag gaaattgttc
     6061 ctcatctgaa tgaacacgac cataaacggt tcagtaaagg ccttgccagg gttttcatcg
     6121 ataattatgc ggcaatccct tcagagtcta ttcgtcgact actggcctta cgtgaagcgg
     6181 ggatctgaaa tccctgatgt tggcgaagat tatacgttac agcaacccga agatattcgt
     6241 ggacgcgtag cgttcggcgc gttgccctgg ttgatgcacg accagccttt cgttcaggga
     6301 cttacggcat gtgcagaaat tggtgaggcg atggctcggg cggtcgtaaa gcctgcatcc
     6361 cgtgctcgtc ggcgtctttc gtttgattaa aggtaaagct atcagcactc aacaaactga
     6421 tgctgttcat taagacgata gcggactccg gttcgaatat tgttttcacc ttcaatggcg
     6481 acggcgaagc gaactctttc gccgtcatga tatgccagtg cggcgcttcc ccctagcccg
     6541 agaataattg agtcgaccac gcccgtgctc actaccgttg aattttcacc gctggcaatg
     6601 atgtgtacat tgtcgccgct gttggcaatt ctggcgtttg cgccaaagct ggcgatttgc
     6661 accaggtcac cattactggc gacatgacac cgttcaccca atgtacaaac gcgtactcgc
     6721 attccggtat tcgcaatccg actactatct ccagcgctgc ttatccgcga agaattaccg
     6781 gttacagcaa cccgtgcgcg ttcaccggag ctgccaatgt gactgttaaa gccgacactt
     6841 ccaatccgca cgctataacc catactggca atttgcgcgg catacccagc ggttgcgatt
     6901 cgggcgttat cctcacaaca gccgatgcga gcatgatcgt cgctgcgggc aaactgactg
     6961 caaaatggta tctctccggg gtctgtggcc agtttttgca tagaggaaac ttcctgatgc
     7021 gcgaagtttt catccgccag ccattttttc cacgcatact caaccagact ctccgcccag
     7081 tccgtatatc cttcctcaat cagtgcatga cgaatatcgg cataactacc gccgcgtggg
     7141 aaatggcgca ggaaccagcg atacatcacc gctccgatac gccattctcg caggtctgct
     7201 cgtgtaatga tcattcgtta gtgccggtga tagccatcga ccatgctttt aaaggtttcg
     7261 tgtggcgtac gccccgtggt gcaaagataa agatgaccga agataaaaaa gaggcttata
     7321 aatgccagag caaaatgcgc ctgcaataac cagtatctta cgccaggaaa cacatctccc
     7381 acggcttgcg gatagagaca caacagcccc gtcaatagta acaacggcag caatccatac
     7441 atgacaccaa cataggcgac ctgctgtaag ggattaaatt tagactgtgt tgttgccggg
     7501 aaaggatgtt cttccccctg cataatgcca aacaaataaa atcgcgtttg ttttgccgct
     7561 cgttccagcc acccctgacg acgaatgcga tagtggtgac cattatcccc aacggcattg
     7621 atcagcacaa agccgagcca gcatgccagt aacaaaaatc cgcaaacttc atgcaccgca
     7681 accagacttt taaccgcagt tgcgcccacc atcgcaaagt gatttatcag cccacttgcc
     7741 agcaacaata caaagagtaa cgcattcgac cagtgccata atctgaccgc tttgctgtaa
     7801 agataaacct tctcaccatg atcggtggct gatttcttca cgccacgagc acgaagcaag
     7861 gcgtgcagcc ccagcaccaa ccacatccca accagcaata ctccggcaat gatcaaccac
     7921 accggccaaa attcgggagt gaactgtggc acatagttcg ctaactggct ctgaaactgt
     7981 tcagcatgtt gcgacgggtt catacatttt cctttttaat caaatgttga ccgaaccggc
     8041 gatacagatg cggttttccg gcaccaggta gctgatattg atagtattta ttatcctgca
     8101 accacgcctg aatttctggg ctatcttcgc gaccaaagat caatgcgtgt tccggacagg
     8161 cgctgacgca aatgggcgga aagccttttg ccagtcggga ctcagcgcag aaatcacatt
     8221 tatctgccac tttagtgaca gggttaaggt agcgaaccag gtacggacaa gcgccaatgc
     8281 aataactgca gccgatgcac tgcgattttt ctacccgcac gatcccctgc tcatcacgcc
     8341 acgatgcccc cgtcgggcat acgtcgatgc acggagcatc ttcgcagtgc tgacatgact
     8401 ggcgaaaaaa gtgatactgc gtttcgttgt cgttatcggt gactggaatg tgtgctatcg
     8461 ataaacggct tccctgagcg gggacgtggt tagttttacg gcatgcgcgg gcgcaaatat
     8521 tacagccatt gcaccgtgac tcgtcatgaa tcatggcata ccgaacttcc ttttcttgcc
     8581 tcgtgttcgc taacagagaa gacgcagaac cagtaaaaaa gattactgtt cccatcccca
     8641 gaacaaattt gcgtcgagtg aaggacatct gattatcccc taatgcagtg gctgcaactg
     8701 tccaccctat ccatgacatc tccctgaaat ttcaggggtt ctgaaactgc tctacctcga
     8761 accattcgca taagccataa gccttgccat aggtcataaa tcgctcgcta aacaagtaca
     8821 acaccgccgt cgatgactcc acctgccgta tatcggcata ttcaggtgca acatccatcg
     8881 cggcgatcgc ggcttc
//
LOCUS       DAZSNM010000171         8861 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZSNM010000171 DAZSNM010000000
VERSION     DAZSNM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8861
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.171"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..396)
                     /gene="yhaJ"
                     /locus_tag="ACHW42_003242"
     CDS             complement(<1..396)
                     /gene="yhaJ"
                     /locus_tag="ACHW42_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFR8944939.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITE"
     gene            501..1202
                     /locus_tag="ACHW42_003243"
     CDS             501..1202
                     /locus_tag="ACHW42_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR8944940.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1225..1389
                     /gene="yhaL"
                     /locus_tag="ACHW42_003244"
     CDS             1225..1389
                     /gene="yhaL"
                     /locus_tag="ACHW42_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR8944941.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1601..2911)
                     /gene="cyuA"
                     /locus_tag="ACHW42_003245"
     CDS             complement(1601..2911)
                     /gene="cyuA"
                     /locus_tag="ACHW42_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR8944942.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2939..4270)
                     /locus_tag="ACHW42_003246"
     CDS             complement(2939..4270)
                     /locus_tag="ACHW42_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8944943.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(4545..5909)
                     /gene="tdcG"
                     /locus_tag="ACHW42_003247"
     CDS             complement(4545..5909)
                     /gene="tdcG"
                     /locus_tag="ACHW42_003247"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR8944944.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(5981..6370)
                     /locus_tag="ACHW42_003248"
     CDS             complement(5981..6370)
                     /locus_tag="ACHW42_003248"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR8944945.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6384..8678)
                     /gene="tdcE"
                     /locus_tag="ACHW42_003249"
     CDS             complement(6384..8678)
                     /gene="tdcE"
                     /locus_tag="ACHW42_003249"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR8944946.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(8712..>8861)
                     /locus_tag="ACHW42_003250"
     CDS             complement(8712..>8861)
                     /locus_tag="ACHW42_003250"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="catalyzes the formation of propanoyl phosphate from
                     propanoate and ATP; TdcD also has acetate kinase
                     activities and functions in anaerobic threonine
                     catabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate/acetate kinase"
                     /protein_id="HFR8944947.1"
                     /translation="NNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHLGKVNA
                     PAEFA"
BASE COUNT         2200 a         2478 c         2145 g         2038 t
ORIGIN      
        1 ttctgtgatg attgccagtt gggtattggc ttttgccgcc agtttgtcga ttaacgggaa
       61 aaaggcaggt gtcggtacca gcgcttcggt cacaatggtg agatgcgttt cccaaccgcg
      121 cgcgagagct tccgcatcag tagtcagttt atctgcggct tccagcaaaa cgcgccctcg
      181 ctccagcaac atcctcccga cattggtgaa tttggtgcga tggcccgagc ggtcaaacag
      241 caccacatcc agctcttctt ccagtttttg catggtgtag ctaagtgcgg aaggcacgcg
      301 tcccagctca tccgctgccg ccgcaaaact gccccggcga tcgatcgcat ccataacccg
      361 tagtgcttcc agcgttaatg ccctttcttt ggccatttcg ttctcattca ggaaatttga
      421 acataccggg cagaatatct ggctaacaat gtagcgtcca gccccttacc ataaaaggaa
      481 gtaaagagag gtcaagaatt atgattacta cccgaactgc caggcagtgt ggacaagcag
      541 actacggatg gttgcaggcc cggtatactt tttcctttgg acactacttc gacccgaaat
      601 tgttaggcta tgcctccctg cgtgtgctta accaggaagt gctggcccca ggtgccgcct
      661 ttcagccgcg aacctatccc aaagtcgata ttttaaatgt gattctggat ggagaagcag
      721 agtatcgcga cagcgaaggc aatcatgttc aggccagcgc cggagaggcg ttgctgctct
      781 ctacccagcc gggtgtcagc tatagcgaac acaatctcag caaagacaaa ccgttaacgc
      841 gaatgcagct ttggctggat acctgcccgc agcgagagaa tccgctgatt caaaagctgg
      901 cgcttaatat ggacaagcag caattaatcg cctcgccaga gggtgcgatg ggaagcctgc
      961 aattacgcca acaagtgtgg ctgcaccata tcgtgctcga caaaggcgaa agtgcgaatt
     1021 tccagttgca tgggccacgc gcgtatttgc aatcgattca cgggaaattt catgcgctta
     1081 cgcatcatga agagaaagcg gcgctaacct gcggtgatgg ggcgtttatt cgtgacgagg
     1141 ctaacattac gctggttgcc gattccccac tgcgcgcttt gctgatagat ttgcctgtct
     1201 agttgttttt acagggagat gatgatgagt aaaaaatcga ccaaaaagcg ccagccagcg
     1261 aagcccgtgg tggcgaaaga acctgctcgc accgccaaaa attttggcta tgaagagatg
     1321 ttgagcgagc tggaagctat cgtcgcggat gctgaaacgc gtttagccga ggatgaagct
     1381 accgcgtaaa gacaggttgc cggatgcggc gtaaacgcct tatccggcct acaaaacaca
     1441 acaacatcaa caaattaaac accccgtagg ccggataaga tgcgccagca tcgcatccgg
     1501 catcaggtac aaaacacaac aacatcaaca agttgagcac cccgtaggcc ggataaggtg
     1561 cgccagcatc gcatccggca caatactcaa aacctggcgt ttatctggcc ttgctcgcca
     1621 taatctcgat aatctgccga tccgtttgct gcatcgaatg gcttgctaac gcacacaggt
     1681 tggcaatcga ctgctcaaca tcatgcgcca cgatcccttc attgccggtc acggcggtat
     1741 catccagcgc cattaacacc gctttccacg cagccgaagc actggtcgaa accttcatcg
     1801 cgcagctgtt cgacgcacca tcgcaaatca tgccgctgac atcgccgatc atactgctga
     1861 tcgccatcga gatagtttca taacgcccat ccaccagcca tgccatcccg gcggcggccc
     1921 ccattgctgc ggtcgttgcg gcacaaagtg cagacaaacg cggtaactgg ttatggatgt
     1981 aaattgcgct caaatgagaa agcatcagcg cacgcgccag tcgttcatca tccgctccga
     2041 agtgttctgc tacaaccacc acaggcatta ttgcagtgat cccctggtta cccgagccgg
     2101 agttactcat agccggaagc gtagcgccgc ccatacgcgc atcggatgcc gcgctggtac
     2161 gaatcacaat ggatgaagag agatctttcg ccagcaaacc gcgctcgcac tgtttttcca
     2221 gcgtcgcgcc aatatgcagc ccccacttac cgctcaaacc ttcctgcgat aacgcacaat
     2281 ttagcttcgc ggaatcgaga ataaagcgga tcgccgcaaa tgggacttca ttgacgaact
     2341 tcaggatctc agccagcgtc gttctggaaa gcaccgtcag cggcgactct tgctcgccct
     2401 ctgccacaca cgcctggtgg gtaaacacca caccattgtg cgtctcaata tgcacaatgt
     2461 tggtatgccc gccgacgatg gtgacacacg cccacttctc accgttccag actttggcgc
     2521 gtgagaagag gatttcattg caaggttcct ggatcttaac agagactttc cccgccgcca
     2581 gcagtgcttt ggcatcggta attgcctgcg cagttgcgtc tttcagcact tccagcccgg
     2641 cgttggcatt tccacctaac gcccccagcg ccgccgcaat cggcagcccc accattcccg
     2701 tgccgggaac ggtgacgccc agaccgttct tcatcagatt tggcgaaacc caggcttcta
     2761 cacgttcaac cggaccttcc agttctgccg cagcaaccgc cgccgccagc gccagtgaaa
     2821 tcggttcagt acatcccagc gccggtttta cttcctcctg aacggcgagg atgtaacgct
     2881 gccataacgg atttaaagtc gaatcaaaca tattaaaaac cttaaaattt caggtaaatc
     2941 aagaaaatgc caggaacaga gaaacacaaa gcaacaaacc agtgacgata atcaggtaca
     3001 gagacatccc tttgtatttg tgcaatgccg gtactttgta aaccagccac gccgggatca
     3061 ggcaccctac catgccgaaa atcgggctac agatagaggt gaagctcaac accggtgcgt
     3121 tcagtacgat ggcgctccag gccagcaaaa tggcgaaaat catgatgccg cgctgaacaa
     3181 gattttcgtt aatcttctcg gcaggcatct tgcgacgcag gatgttcatt acgatccctt
     3241 gcgttgcttc gcgaaagcct aaatagacgc caaagaatgc agtcatgaca gcaaagatat
     3301 tgagaatgac gctgaccact ttcacccacg ctgcgccgtc accgctaata aactgtgcgg
     3361 caatcgccag cgcggaaata ttctgctcgt aggcttttac cgcttcgtca tgtcccatcg
     3421 ccagcgtgaa cgacacggcg tagaaaaaga cggtgacaaa caaaatgcca aacgcgatat
     3481 tcattgcccg caatgcttta tgccgcgcca cttcaattga tttttcccgc gagcgataag
     3541 agatcaccat cgggcttaac gtctggataa acagaatcga cgtcagggta aacggcagcg
     3601 taataatggc gtttttcacc agcagcccca gcggcggtag cgaaccgacg ttatacagat
     3661 gccacattcc caccatcgac acgcccagcg cagcgactac cagcagcttg gtcagcacca
     3721 tgtcggtcga aattttgaat aacaatttct cgccgcgtga agagatcgcc accagaatgc
     3781 aaatcagtac cagaccataa aagggactgt ctgacagcaa cccttccgtc acgccgaagg
     3841 tatgcagata ggaagcacta tcgttggtga tggcggtgga atagacgaac atccaaatca
     3901 ccagcattac gaaatagagc gcacctaaca ggatgcccca gtttttacct aaataaccgc
     3961 taatgacact cgggtaatct ttacattctg gtgattctgc cagcgtatta ataaacaacc
     4021 gctgaaacag atacattgcc gggtaaccaa tcaccgatga gagcaaaaat acccacaatc
     4081 ccatcaaacc gacctgcacc gggagaaaaa caatccccgc gccaatcgcc atcccgatac
     4141 tcataatcac ccagccggtg tcagtactgt cgaatttgat cgcttctcgc cactcgctct
     4201 cactcattcc agcacgaccc gccggggtcg aagcgtctgc aatgacgcct ttattcgatg
     4261 caatttccat aatttctcgc tcaatatttt gtagggctta ttattttttt ccgagccgca
     4321 tcaaggcgat atgcggtcta cgtttttgca ggcgagttat tagaataaag aaatgatacg
     4381 cgcgaggtca gagaaaatct tcacaatcaa gactttaaaa taacggagat ggataagaat
     4441 tttctactaa attaatcaca agagaatatt cattctctat ttgcggcaga tcacaaaaaa
     4501 aaggtgcaca tttgtgcacc caaggatgaa agctgacagc aatgtcagcc gcagaccact
     4561 ttaatggcca gtcctccgcg tgatgtttcg cggtatttat cgttcatatc tttgccggtt
     4621 tcatacatcg tctcgatcac tttatcgagt gaaacacgcg gtgccgaggt gcggcgcatc
     4681 gccatccgcg cggcgtttac tgctttcacg gcattaatgg cattacgttc aatgcacggg
     4741 atttgtacct gtccggcaac cggatcgcag gtcagcccaa ggttatgctc catcgcgatt
     4801 tccgccgcat tgcatacctg cgccggactg ccgcccagta gttcagttaa ccctgccgct
     4861 gccattgaac aggccacgcc aatctccccc tgacagccga cctccgcgcc agagatggag
     4921 gcgttcattt tatacagcgc gccaatagcc cccgctgcca gaaaatagcg ggcaattgac
     4981 cgctcgttta ccggacgacg gaacttatcg taataagcca gcactgccgg aataatgccg
     5041 cacgcaccgt tagtcggtgc cgttaccacg cgcccgccag ctgcgttttc ttcactaacc
     5101 gccagcgcgt acatgttgat ccagtcgatg acattcatcg gatcgttaga gatgttatcg
     5161 ctagaaacca gctgacgacg cagcgctacg gcacgacacg gcacattgag cggaccaggc
     5221 agcacgcctt cagtgttcat cccgcgttca ataccgtcat gcatcacttg ccagatacgg
     5281 gcaaaccctg cgtcaatttc cgctttgctg cgcagcgcca gctcgttgtg catcatcagg
     5341 ccagatatag acaggccgtt gtaatcacac attttcagca gttcacctgc tgagtggaaa
     5401 tcgtaaggta cggacgtttc gacatcgtgc gacaggccga agtgttcttc ttcgacaata
     5461 aacccgccgc cgacagagta ataggtttta cttaatagcg cttcctgtcc cttccaggca
     5521 gtgatccgca ttccgttctc atggcgaggc aacatttcgg gatggaagat aatgttcttt
     5581 gctacaggaa aatcaacaat atgcgcacca gatgccactg gcagccgccc gctgcgcgtt
     5641 actaactcta taaatgcagg gatctcatca atgacaacat cctgcggact gtttcctgcc
     5701 agtcccatga taatggcgac atccgtggca tggcctttgc ccgtcagtga caacgacccg
     5761 tacagatcga ccacaatatg gctcgtcgcg gttaataagc cgctactttc cagccgatca
     5821 ataaaacttt ttccggcatt cattggcccc acggtatgcg aactggaggg accaatccca
     5881 attttgaaaa tatcgaatgc actaatcata tccacaccct cggattgccg ttcagtgaag
     5941 tggagcggaa cgaccttgcg accgtcccgc tcacgaggct ttacgcacta cgtactgcga
     6001 tagcttcaat ttccagcttc acatctttcg gcaaacgcgc aacctgcaca cagctccggg
     6061 tcgggtaggt cgcctgatgc tcatcgaaga actgcttata gacttcattg atggtggcaa
     6121 aatcattcag atcggtgatg aacacggtca tcttgatgat atcgcccacg ctcagcccgg
     6181 cagcaaccac gatcgctttg acgttttcga ggcttaaacg cgcctgatct tgcacatcag
     6241 ccgggatctc accggtctgc gggcaaaccg gaatttgccc ggaggtgaag accatgctgc
     6301 ctaaatcaac gccctgaaca taagggccga ttgcgcctgg ggcacgttgc gtttcgataa
     6361 tctttttcat acatcctccg gcgtcagagc gcctgggtaa aagtacgtga aataacatcc
     6421 tgttgctgtt cacgggtcag tgcgttgaag cgcacggcgt agccagagac acggattgtc
     6481 aggttaggat atttttccgg gtgctcgatg gcatccagca gcatttcccg attcattacg
     6541 ttgacgttga ggtgttgacc gccttcgaca tccgcttcgt ggtggaaata cccatccagc
     6601 aggccgacaa ggttggtttt acgtactgga tcttctttgc ccagcgccgc aggaacgatt
     6661 gagaaggtgt acgagatccc atctttggcg taggtgaacg gcagtttcgc caccgacgtc
     6721 aatgaggcca cggcaccttt gcggtcacga ccatgcatcg ggttagcgcc cggcgcgaac
     6781 ggtgttccgg cgcgacgacc gtccggcgta ttaccggttt tctggccgta caccacgtta
     6841 gaagtgatag tcagaatcga ctgggtaggg acggcgttgc gataggttgg cagcgcttta
     6901 attttcttca taaagcgttc aaccaggtcg caggcaatgc tgtctacgcg ctcgtcgttg
     6961 ttgccgtact gcggatattc accgtcgatt tcaaagtcca ccgccaggcc gttttcgtca
     7021 cggattggtt tcacgcgggc atatttgatg gcagacaggg agtccgtcgc caccgacagg
     7081 cccgcgatgc cgcatgccat agtgcgatag acatcacgat cgtgcagcgc catcagcgaa
     7141 gcttcgtagc tgtacttgtc gtgcatgtag tgaatgatat tcagcgcgct gatgtactgc
     7201 accgccagcc agtccatgaa gtgatcgagg ctgtccatca ctttgtcgta atccagcacg
     7261 tcgtccatca gcggtgctgt tttcggcccg acctgaatct tcagcttctc gtccaccccg
     7321 ccgttaattg cgtagagcag cgttttcgcc aggttagcgc gtgcaccaaa gaactgcatt
     7381 tgcttaccaa tcaccattgg gctgacgcag caggcaatcg cgtaatcgtc gctgttgaag
     7441 tcagtacgca tcagatcgtc attttcatac tgcaaggaag aggtgacgat cgacacctgc
     7501 gcggcatatt ttttgaaggc aatcggtaat tcttccgacc aaagaatggt caggttaggt
     7561 tccggtgccg gccccatagt gtgcagggtg tgcaaatagc ggaaggagtt tttggtcacc
     7621 agcgtacgac cgtccagccc catcccgccg atcacttccg tcgcccagat tgggtcgccg
     7681 gagaacagcg aatcaaattc cggtgtacgc aggaagcgta ccatacggat cttcatgatg
     7741 aagtgatcga tcagttcctg tgcctgctgc tcattgagga cgccagcttt aaagtcgcgc
     7801 tcaatgtaga tatccaggaa cgatgccgtg cgccccagcg acatcgcgcc gccattttgc
     7861 gatttcactg ccgccagata agcgaagtag agccactgca ccgcttcctg tgcattctgc
     7921 gccgggcgag agatatcaaa gccatatttc gccgccattt cctgaatctg caacagcgca
     7981 tgacgatgct ctgccagctc ctcacgcaga cggatggtgg cttccagatc ctcgcctttt
     8041 tccagacgag actggagatc ggcaaattgc agttcgcgtt cacgcaccag ataactgatg
     8101 ccatacagcg ctacgcggcg atagtcaccg ataatgcgcc cacgaccata gccatctggt
     8161 aaaccggtca gcacgccaga tttacggcag cgcagcatat ccggtgagta aacatcaaat
     8221 acgccctggt tatgggtttt acgcagatcg gtaaacagat attcaaattc actgtccatt
     8281 tcacggccat aggcgtggaa tgaactttta atcatattaa tgccaccgaa cgggtgtagc
     8341 gcacgtttca acggcgcatc cgtttgcagg ccaacaattt tttccagcgg ctggttaata
     8401 tatcccgcat catgagcggt aattgtggtg gcaatattgg tatcgaaatc aaccggcgcg
     8461 tgggttgcgt tttcgatacg gatgccttcc attacctttt cccacaattc cgtggtggca
     8521 ggcgtcgctt cggcgagaaa agattcatcg ccttcatacg gtgtatagtt atgttgaata
     8581 aaatcgcgga cattaatttc gcttttccag tccgtacctt taaagccaag ccatgcgtca
     8641 gcgtacagct tatcgctggt atcaatatct accttcatga aaaataatct ctctacaata
     8701 cttcaactaa attatgcaaa ttctgcgggc gcgttaactt tacctaaatg aatggcatcc
     8761 aaagcaatca ttttttcttc gttagtcgga ataacgacgc aaatgacatg cgcattttca
     8821 ctggaaacaa ttcgctcgcc aaaagagtta gaacgattat t
//
LOCUS       DAZSNM010000172         8802 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZSNM010000172 DAZSNM010000000
VERSION     DAZSNM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.172"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..2142
                     /locus_tag="ACHW42_003251"
                     /pseudo
     CDS             1..2142
                     /locus_tag="ACHW42_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(2111..3848)
                     /gene="aceK"
                     /locus_tag="ACHW42_003252"
                     /pseudo
     CDS             complement(2111..3848)
                     /gene="aceK"
                     /locus_tag="ACHW42_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4032..5336)
                     /gene="aceA"
                     /locus_tag="ACHW42_003253"
     CDS             complement(4032..5336)
                     /gene="aceA"
                     /locus_tag="ACHW42_003253"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFR8944948.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5366..6967)
                     /gene="aceB"
                     /locus_tag="ACHW42_003254"
                     /pseudo
     CDS             complement(5366..6967)
                     /gene="aceB"
                     /locus_tag="ACHW42_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7236..8165)
                     /gene="metA"
                     /locus_tag="ACHW42_003255"
     CDS             complement(7236..8165)
                     /gene="metA"
                     /locus_tag="ACHW42_003255"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR8944949.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8322..8765
                     /locus_tag="ACHW42_003256"
     CDS             8322..8765
                     /locus_tag="ACHW42_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR8944950.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2296 a         2135 c         2144 g         2227 t
ORIGIN      
        1 ataatacagc ccagacaaat gaacaccaaa ccctgagtga attgttttat aaagaactcg
       61 aggataaatt ttctggcaag gagctggcga cgcctctatt aaaaagcttc tcagagaact
      121 gtagacaaaa tgttcgtcat atctttagca acaaggattt tgtcattaaa ttttccacgt
      181 ccgtcttaca agctgataag aaagaaatta cgatgattaa taaaaacgaa aacacgacac
      241 tcactcaaac cattgctaca atatttgaag aatacctaat ggaaatttta cctcaacgct
      301 cagacactct tgataaacaa gaattaaacc aaaaatcaga tagaaaagaa aaagaattcc
      361 caagaattaa acttaatggt caatgttatt tcccggggcg ccccaaaacc gtatagtata
      421 ccgacacatt gctgcacaat atattaatga tatttatcag aatgttgatt acaaacccca
      481 tcaagatgat tactcttcag ctgaaaaatt tctcactcac ttcaacaaaa aatgcaaaaa
      541 ccagactttg gcgttgattt ccagccgtcc tgaggggcgt tgcgttgctg cctgcggtga
      601 tttcgggcta gttatgaaag catattttga caagatggaa tcaaatggca tcagtgttat
      661 ggcagccata ttactggtgg ataaccatgc tttgacggtc cggctaagaa taaagaacac
      721 aactgaagga tgtacccatt acgtggtttc ggtttatgat cctaatgtaa ctaacgataa
      781 aataagaatt atgagcgaaa gcaaagagga tattaaacac tattctctga tggattttat
      841 gaatgtagat tatagcctcc tgaaatggtc aaatgatcat gttattaacc aatctgttgc
      901 aataattcca gcacttccga aagaacagct attgatgtta aaaggaactg tggatgaaat
      961 aacccctcca ttatcaccag caacgatgaa tttgctaatg gcaattggtc agaatcacca
     1021 acttacgcaa ctgatgattc agctccagaa aatgccagaa ctacatagaa cagaaatgtt
     1081 gactgcctat aatagtatta acctccccgg tttatatttg gctataaatt atggcaatgc
     1141 ggatatcgtt gagactattt tcaattcatt gtcagaaaca ggatatgaag ggttactctc
     1201 gaaaaaaaat ctcatgcata ttctggaggc aaaagataaa aatggttttt ctggattatt
     1261 tttagcgata tcacgtaagg ataaaaatgt tgtaacctcg attctgaacg tcttacctaa
     1321 actggccgca acacatcatt tagataatga acaggtgtat aaattcctga gtgccaaaaa
     1381 tagaacgtcc agccatgttt tataccatgt tatggcgaat ggtgatgccg acatgctgaa
     1441 aatttttttg gtcgcgttac ctttgttaat tcgcacatgt catttgacta aagaacaggt
     1501 actcgatctc ctgaaggcaa aggattttta tggttgccca agactatacc tggcgatgca
     1561 aaatggacat agcgatatcg tgaaagttat tctcgaagca ttgccctgtc tggcccagga
     1621 aattaacatt tcagcttccg atattgtaga tcttctgacc gctaaaagtc ttgcgcgcga
     1681 cacgggtttg tttatggcca tgcagcgcgg acatatgaac gttattaata ctatttttaa
     1741 cgcattaccc actcttttta atacgtttaa attcgataaa aaaatatgaa gcccctcctc
     1801 ctggcaaata attctaatga atatcccggt ttgttttcag cgatacagca taaacaacaa
     1861 aacgttgtag agacggttta tcttgcttta tctgaccatg cacgcctgtt tggatttacc
     1921 gctgaagata ttatggattt ttggcaacac aaagcgccac aaaaatactc tgcctttgag
     1981 ttggcttttg aatttggtca ccgggttatt gctgaattaa tccttaatac attaaataag
     2041 atggctgaaa gctttggctt tacggataac cctcgataca ttgcggagaa aaattatatg
     2101 gaagctttac tcaaaaaagc atctccccat accgtacgct aaatctttgc ctgcgccgat
     2161 acgcataaac atcttccaca tgcccttcac gtatgcggtt ttgtagtgcg cgccagtaat
     2221 cagcgcggaa caggtcggcg tgcatctctt caaacagcgg accaatacgc gggtcggcgc
     2281 atagccagtg gcgaaactct tccgggaaaa catcgcccgg cgagacgctg taccacggtt
     2341 cgctggcaag ttcgtcttcc ggatagcgcg gcggcgggat gtcgcggaaa ttcacttccg
     2401 tcatgtagca aatttcatcg taatcataaa aaaccacacg cccgtgacgg gtgacaccga
     2461 agtttttaaa cagcatgtcg ccagggaaaa tgttagcagc ggcaagctgg cgaatagcgt
     2521 taccgtattc ttcaatggcg tcgcgcaact gctgaccttc cacttgttcc agccagatat
     2581 tgagcggcac catccgccgc tcgattatag agatggcgaa ttacaatctg ctcaccaaga
     2641 tcggtgattt tttccgctgc ttcctggagc agtaattcca ttaataccgg ggaaatatgc
     2701 cgcttctcca gcacaaagtt ttcaaactcc tgggtgtccg ccattcggcc cacacggtcg
     2761 tgctctttca ccagttgata gcaggcacga acgtgagcgg cagacatctc tttctgcggc
     2821 gcgaacttgt ctttgatgac tttgaatacc cgatcaaagc ccggcagcgt aaacaccaac
     2881 atcaccattc cacgaatacc cggcgcttca atgaactgct cattacagcc ctgtagataa
     2941 acgagatatt cgcggtagct ttcggttttg gcgtgcttct ggcagccgat agccatatac
     3001 aattcagcag tggttttacc tggcagaatt tcccgtagcc actcgaccag cgctgcgggc
     3061 agcggtgcat aaaccataaa ataagaacgc gcaaagccaa aaacaatgct cgcttcggcg
     3121 gtcgtcgtca ggcaggtatc aataaataac tcgccgtcgt ccgtctggtg gatcggcagt
     3181 aagaagggca acgtgccgga aggcgtgatc agtttgccca ccagccaggc ggctttattg
     3241 cggtaaaaca gttcgttcgc cacctgtaaa tgactttccg cgaggttgtc tgtccccagc
     3301 gtttccgtca gatggcgaat gatgtaatga atgtcacggc ttttattctg ccagcgcagg
     3361 cgcagcggca ggtcgctgat aacgcgcatc agtagagatt cccagccgtg atcggggtga
     3421 aagtctttcg ccagcgggcg gggaatggta cgaaagcggc gctctggctg agagctaaag
     3481 ataaaaagcc gctcgggagt aagcgagcgg tggtcaaata accgacagta cacggagtta
     3541 aaaaagctct ccgcaatctc gaagcgcggg taatccggca acagccgcgt gtaatgctct
     3601 ttaacgcgca gtaaaaatgc cgcgtccgta ctttggccgt tagtaatgca gcgcagttgc
     3661 tccacgacca gaccaacgtg atgatcgtaa agatggatac ggtttttcat cgcctgctgg
     3721 acagcatgcc agtcggcctg ttcgaaacgc tgctgcgccc cggaagtcac ttcgaggaat
     3781 cgaccatact gagcgtcgaa gccttgcaaa atggtttgag caatcagtaa ttccaggcca
     3841 cgcggcattt agcgccctca tcaggagcag agaattgccg gatgcggcgt aaacgcctta
     3901 tccagcctac gttcggcaac ggttgtaggc atgataagac gcgcaagcgt cgcatcaggc
     3961 atcgtgcacc gattgccgga tgcggcgtaa atgccttatc cggcctacag tcagcaacgt
     4021 ttgtaggttg cttagaactg cgattcttca gtggagccag tcagcgcggt gactgaagac
     4081 gtgccgccct gaataatagt cgtcacttta tcgaagtaac ctgtacccac ttcctgctgg
     4141 tgagatacga aggtatagcc atctttcgcg gcggcaaatt ccggctgctg cactttctca
     4201 acatagtgct tcataccttc gccctgcgca taagcgtttg ccaggtcgaa catgttaaac
     4261 cacatgctgt ggatacctgc tagggtgatg aactggaact tgtagcccat atccgacagc
     4321 tgctgctgga agctggcaat agttttgtcg tcgaggtttt tctgccagtt gaacgacggc
     4381 gagcagttat aagccagcag tttgcccgga tatttcgcgt ggatagcttg tgcaaagcga
     4441 cgcgccagtt ccagatccgg cgtggaggtt tcacaccaga ccaggtcagc atatggcgca
     4501 tacgccaggc cacggctgat cgcttgctca atgcccgcat gagtacggaa gaagccttca
     4561 ctggtacgct cgccggtaat aaattcgctg tcatacgggt cgcaatctga ggtgatcaga
     4621 tccgccgcat cagcatcggt acgtgcaacc agcagggttg gaacgcccgt cacgtcagct
     4681 gccagacgcg ccgcgaccag tttctgaata gcttcctgag ttggcactaa aactttgccg
     4741 cccatgtgac cgcatttctt cactgacgcc agctgatctt cgaagtgaac tgccgcagca
     4801 ccggcttcaa tcatcgcttt catcagttca aaggcattca ggacaccgcc aaaaccggct
     4861 tccgcatcgg caacgatcgg caggaagtaa tcgacatagc gcggatcgcc cggctcaatg
     4921 cccacggacc attggatctg atcggcacga cggaaggtgt tgttgatccg ctccaccaca
     4981 gctggcaccg agtttgccgg atagagcgac tgatccggat acatgctggc cgccaggtta
     5041 gcgtccgccg ctacctgcca tcccgacaga tagactgctt caatacccgc tttcgcctgt
     5101 tgcagcgcct gaccgccagt cagtgcaccg aggttgttga tgtagccttt tttcgactca
     5161 ccgtgcagca gacgccacat tttcgctgcg cccagttgcg ccagcgtgca ttcaggattg
     5221 actgaaccgc gtaatttcac cacatcttcc gcactgtatg ggcgagtaat gccttcccaa
     5281 cgcggttgag tccactcttt ctgtaattct tcaatttgtt gtgtacgggt tttcatgtgc
     5341 agatgctcca tagttatgtg gtggtttacg ctaacaggcg gtagcctggc agggtcagga
     5401 aatcaattaa ctcatcggaa gtggtgatct gttccatcaa gcgtgcggca tcgtcaaaac
     5461 ggccctggga gaaacgttct tcgcccagtt cgctggcaat gactttcatc tcttcgccca
     5521 gcatctggcg gaacaaggct ttggtcaccg gtttgccatt gctcaacgtt ttttgatgat
     5581 ggatccactg ccagatcgag gtacgggaaa tttcagccgt cgccgcatct tccatcaggc
     5641 cataaatcgg cacgcagccg ttgccggaga tccacgcttc gatgtactgc acagccacgc
     5701 gaatgttagc gcgcatacct tcttcggtgc gttcaccatc acaaggtgcc agcagctgat
     5761 cggcagtaat cggcgcgtct tgttcgcgca atacttcaag ctgattttta cgggagccga
     5821 gaatgtcgtt gaataccgcc attgccgtgt ccgcaaggcc tgggtgagcg atccatgtgc
     5881 catcgtgacc gttattggct tccagcgctt tatccgcttt tactttgttg agcacctggt
     5941 tattacgctc ttcatctttg ctcggaataa acgcggccat gccgcccatc gcaaaagcac
     6001 cgcgtttatg gcaggtttta atcagcaggc gtgagtaagc attcaggaat ggtttatcca
     6061 tcgtcacggc ctgtctgtct ggcaggacac gatcgggata gtttttcaac gttttgatat
     6121 agctgaagat gtaatcccag cgaccgcagt tcagaccaac aatatggtcg cgcagcgcgt
     6181 gaaggatttc gtccatctgg aacacggcgg gcagcgtttc aatcagcaac gtcgccttga
     6241 tggtgccgcg cggcagatta aagcgatctt ctgcatagct gaagacttcg ctccaccagg
     6301 ccgcttcctg ctaggactgg gttttcggca gatagaaata gggaccgctg ccctttgcca
     6361 acagtgcctg atagttgtgg aagaaataga gcgcaaaatc aaacaggcta ccggggattg
     6421 cctcaccacg ccaggtgaca tgtttttccg gcaagtgcag accgcgtacc cgacaaatca
     6481 aaaccgctgg attgggcttg agctgataaa ttttgcctgc ttcattggta tagctgatgg
     6541 taccgttaac cgcatcgcgc aggttaattt gcccgtcgat cactttgttc cagtctggtg
     6601 ccagtgaatc ttcgaaatcg gccataaaga ctttcacatt ggcgttcagt gcgttgatca
     6661 ccatcttgcg ctctaccggg ccagttatct ctacgcggcg gtcttctaag tccgcaggaa
     6721 tcccgcgaat tttccaatca gcatcgcgaa tggaagccgt ttccgaaata aaatcaggca
     6781 acgttccgtt atcaatatct tgctgctgct gaatgcgcgc tgccagaagt ttattgcgtt
     6841 gtggcgtaaa atgcgtcacc agctcagtca gaaattctac cgcttcggca gtaagaattt
     6901 gcttctcctg ctcgccatac ggccttgtga aagccagttc atcggttgtt gttgcctgtt
     6961 cagtcatcgt gcagctcctc gtcatggatc cgaaaacttc ccctctgtga acgaaggaac
     7021 gttgtgcgct tttcgttcag cacaactaag actactcatt taaaatgcaa aatcaaaaac
     7081 aatttccatt ttaattaaaa atacttgata acttattgat aacaatatga ataaaattta
     7141 ttcacttgct gaggtgcgtt tcggaaataa aaaaggcacc cgaaggtgcc tgaggtaagg
     7201 tgctgaatcg cttaacgatc gactatcaca gaagattaat ccagcgttgg attcatgtgc
     7261 cgtagatcgt atggcgtgat ctgatagacg taatagttga gccagttggt aaacagcaaa
     7321 ttaccgtgac tacgccagct cgctcgcggt ttgttttgcg gatcgttgtg cgggaaatag
     7381 ttatacggta catccgggtc tagtccggct tccacatcgc gtaaatattc ctgcgccagc
     7441 gtttgcgcat catattcggg atggcccgtc acaaaggcaa tgcgcttatc tttgctggca
     7501 aacagatatg catccccttc ttccgtctct gccagaattt ccagatcggt gtaatcacga
     7561 atcaacgctg ccggaaagtc agcatagcgc gaatgcggtg ccaggaatga atcatcaaag
     7621 ccacgcgtca gaagcgcatg aggatggaga atatgatgct cgtaaacgcc agagagtttg
     7681 tcggtgcgag tttgcttagg aatgccgtag aggatattga gcgcggcctg taccgcccag
     7741 cagacaaaca gcgtcgaggt gacgtgatct ttcgaccact ccagcacctg tttgatctgc
     7801 ggccagtaag cgacatcatt aaactccacc aggcccagcg gcgcaccggt tacaatcaga
     7861 ccgtcaaagt tctgttcctg aatatcttca aagttacagt agaagttgtt cagatgctct
     7921 gcgggcgtgt tgcgcgattc acgggaatcg atgcgcaaca gctgaatatc gacctgcaaa
     7981 ggtgagtttg aaagcaggcg cagaaactga ttttcagttt caatcttctt cggcatcagg
     8041 ttaaggatca gaaccttaag tggacgaatt tcctgaccag acgcacgaga agttgtcatc
     8101 acaaagacgt tttcttcacg caagaaattg acggcgggta gctcgtccgg cacacgaatc
     8161 ggcataacct gattacctca ctacatacgc ttatacgttt agacatccag atagctgaag
     8221 ataaccagaa aatttgccaa tgtcgagcct gcatgttgaa ggtgagaaag tttcaaggaa
     8281 aaggctacgt tagaatataa gaatgacgaa aaggagagaa aatggttatt agtattcgcc
     8341 gctcacggca tgaggaaggg gaggaactcg ttgcgatttg gtgtcgatct gtcgatgcca
     8401 cccacgattt tttatcagca gagtatcggg ccgagctgga agagctggtg cgttcctttc
     8461 tgccggaagc accgttgtgg gtcgcggtta atgaacggga gcagccggtt gggtttatgt
     8521 tgttaagtgg gcagcatatg gatgcgctgt ttatcgatcc tgatgtgtgc ggctgcggcg
     8581 tgggtcggat gctggtggag catgcgctct cgatggcacc ggaactgaca accaacgtta
     8641 atgagcaaaa tgagcaggcg gttgggttct ataagaaggt gggttttaag gttacgggac
     8701 gctctgaggt ggacgatttg gggaaaccgt atccgttgct gaatctggcg tatgtggggg
     8761 aataagccgc ctctgccgtg cctgcccaag ctggagaaga cg
//
LOCUS       DAZSNM010000173         8759 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZSNM010000173 DAZSNM010000000
VERSION     DAZSNM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8759
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.173"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..438
                     /locus_tag="ACHW42_003257"
     CDS             <1..438
                     /locus_tag="ACHW42_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR8944951.1"
                     /translation="QKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQ
                     FAVQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYA
                     KAKLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="ACHW42_003258"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="ACHW42_003258"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR8944952.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="ACHW42_003259"
     CDS             complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="ACHW42_003259"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR8944953.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="ACHW42_003260"
     CDS             complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="ACHW42_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR8944954.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4440..5147)
                     /locus_tag="ACHW42_003261"
     CDS             complement(4440..5147)
                     /locus_tag="ACHW42_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR8944955.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="ACHW42_003262"
     CDS             complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="ACHW42_003262"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR8944956.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6005..6208)
                     /locus_tag="ACHW42_003263"
     CDS             complement(6005..6208)
                     /locus_tag="ACHW42_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR8944957.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6367..7875
                     /locus_tag="ACHW42_003264"
                     /pseudo
     CDS             6367..7875
                     /locus_tag="ACHW42_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7872..>8759
                     /locus_tag="ACHW42_003265"
     CDS             7872..>8759
                     /locus_tag="ACHW42_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR8944958.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEP"
BASE COUNT         2032 a         2388 c         2250 g         2089 t
ORIGIN      
        1 cagaaggtgg cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc
       61 gtgttttatg tcgcgggaga taaagtcggc atcgttcgtc tggatgcttt taaagccagt
      121 aaagatattc agtttgcggt gcagtcagga ccaatgttga tggaaaacgg tgtaattaat
      181 ccgcgtattc atcccaacgt cgcctcacgt aaaattcgta acggtgttgg gattaataaa
      241 catgggaatg ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt
      301 tatgccaaag cgaaactgaa cgttgagcag ctgctttatc ttggtggcac gatttcacat
      361 atgtatatga aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc
      421 gtcgagcgga aaggttagaa tgatagcttt gtgtcagata ctaaatatgc tgctgatgcc
      481 ggatgcggcg taaacgcctt atccggccta catgacgttg caatttattg aatctgaatg
      541 attttttagg ccaaataagg tgcgcagcac cgcatccggc aacgttacac cgactccagc
      601 cgggcgtaag ctgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg
      661 ctatgttcac cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgt
      721 acacgctggc cgagcttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga
      781 tggctgaccg ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc
      841 aactcgccaa taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt
      901 tccgcgtagg tcagcgtcag tttctgcatc gcgcgggtta cgccaacgta ggccagacgg
      961 cgttcttctt ccagacgccc gccttcatcc agcgacatct ggcttgggaa catgccctct
     1021 tccataccga cgataaacac ctgcgggaac tccagacctt tcgccgagtg catggtcatc
     1081 agttgtaccg catcctgcca ggtatccgcc tgcccttcgc ctgcttccag cgccgcatgg
     1141 gataggaacg cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc
     1201 gttgccgtca ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc
     1261 tgctcgtaca tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc
     1321 ggcatatcgg cagtttcctg cgctaaggcg tcgatcagtt ccataaaccg ctgtaaggcg
     1381 ctggcagcac gtccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt
     1441 gttaactggc gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc
     1501 cgcgttggcg tattcaccac acgctcaaag gccgcgtcgt cgttacggtt ggcaatcagg
     1561 cgcagatacg agagcgcatc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa
     1621 atacggtacg gcatactcgc ctgcaacagt gcttcttcca gcacacgcga ctgggcgtta
     1681 ctgcggtaga gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg
     1741 cggttaacca caaaacgcgc ttcatcgagt tcgttaaaag cgcaatagag ggaaataggc
     1801 tcaccgtccg cgccatcggt ccacagtttt ttacccagac gcccgttatt gttttcaatc
     1861 agggcgttag cggcgctcag aatattgctg gtagagcggt agttttgctc cagacgaata
     1921 atttcggcac cggggaaatc attaaggaaa cgctgaatat tctccacctg cgccccgcgc
     1981 cagccgtaga ttgactggtc gtcatcaccg acgatcatca ctttgccggt gtcgcccgcc
     2041 agcaggcgga tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggata
     2101 ttggtaaaac gttcgcggta gtgttgtagg atatgcggct tgttaagcca cagttcgtga
     2161 gcgcgcagca gcagctcggc gaagtccacc aggcccgcgc gatcgcacgc ttcctgatac
     2221 gcctgataca ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc
     2281 ggacgcaggc cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac
     2341 tgcttctcgt cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttcg
     2401 ctatcgagga tctggaaatc ctgcggcaga ttggcgtcca tatggtgcgc acgcagcagg
     2461 cggtgcgcca gcccgtggaa ggtgcctacc cacatgccgc cctggctggt gcccatcagt
     2521 tgtccgatac gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc
     2581 gagtatggcg agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc
     2641 gtcttaccac tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct
     2701 tcgcgttgtt tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg
     2761 taaaaatagg ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca
     2821 gatttgctga ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgtggca
     2881 gtaaacggct gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct
     2941 gcattccgct gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga
     3001 tctcgccgat cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt
     3061 ttgagcgccc gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca
     3121 gttccggctg ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt
     3181 ttaaggtgtc gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa
     3241 tcattgctgc gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg
     3301 cttgttcaat cgaacgaaaa cgccaacgtg tcacgtcgtg ataaatctcg ggttccgctt
     3361 cccgtaccgc ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat
     3421 gataattttg cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat
     3481 cgtaaagggt atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa
     3541 aacgcattat ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg
     3601 ttgaaaatca agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagtagctc
     3661 ctgcacacca cgaagatcgc cgctcgactc cagcatatgc gtagcgaacg agtgacgtaa
     3721 tttatgtggg tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt
     3781 ctgcacatta cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc
     3841 gctaccaaac aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc
     3901 aatcggcagg cggcgctctt tgctgccttt ccccatcacc cacacttcgc cggactccag
     3961 gtcgaggtgt ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat
     4021 cacttccagc attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tatccagcag
     4081 ccgattcata tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga
     4141 aactcctttc gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct
     4201 acgtagcgca gaaagccgta acgccagact tgctgctccc agccctttac ggcgactgcg
     4261 tacagcaaaa ttgcgtacca tcgctgcatc gcattgttgc cagctttgca ggccgttttc
     4321 gctggcaaaa ttgatgatcg cctcaagctg acgctggtag ttaagtaggg ttatcgggct
     4381 aagctgacgc tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt
     4441 catacgcgtt caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt
     4501 aactgcgttc cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacaaca
     4561 cccaaatcag catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc
     4621 ggtagcacca ccagcagctc cggtccgtta agcggcccaa gatagtgctg ttcctgcccc
     4681 aaacgctgaa tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc
     4741 gacggcgcac ctaagcgcca gcgatctgga aacaggcgca gactcgcacc agctaggccg
     4801 agatcgcgcg cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg
     4861 agactgcgct gcaggtagag taggcgataa aacagccctt cgttggcgat agcctgctcc
     4921 atcaacaacg ccatgttctc ttccagaaca tgaatgtgat tacgtgcgcg ggccatgtgc
     4981 cactcgacca acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg
     5041 cgcgcattgc ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca
     5101 tcaagctccg tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc
     5161 catcgtagac atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg
     5221 cgatatcaag acgaccgccg gggagttcca cgcgtacttc ttcgtccagc aaaccttgct
     5281 gaatccctac cgccaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc
     5341 cacgctcata aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat
     5401 tggcgcgctc cggaaaacgc tcgtgacttt ccagaacagg accaagcgtt tctaccgccg
     5461 cggtatcgac atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc
     5521 ataagattgt ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa
     5581 acggcacggc ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat
     5641 cggtgacggt cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg
     5701 tcagtccttt cagacgcaca aaacgggcaa agcagcgcgc accgttaccg cactgcgcca
     5761 cttcactgcc atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg
     5821 gctcaaccac cagcagctgg tcaaacccta cccccaggtg ccgatccgcc aggcgacgaa
     5881 tcagctccgg tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc
     5941 caaggccatg cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg
     6001 acttttagta attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca
     6061 ccgtggattg cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag
     6121 gcgggaaata gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata
     6181 cagtgagtgc cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg
     6241 ctttctatca tcgcaggaga aggcgtaaaa gcaagcgttt agcgactctt tatgaagtct
     6301 gaaaagtgag gaaatgctgc gagtataacg cttactcgtg aggatttatg ttctgctggc
     6361 agtttcgtga tcaacacctc gcgccaggat ggctttatgc actatcgcaa tgtggtttgt
     6421 ttgttgtctt gttcattgtt tttatcgtcg gcatggggat gtcggctttc cgcacctgaa
     6481 cataatattt accagaaaca gggaaaaggc gtggtgtacc tgcgccctta taaagaaaca
     6541 aatctttctc tacctgacgt gaattacaag agtttgcgtc ggttacccaa tttattaata
     6601 gacccgacaa cactagacga atgggataaa gaaccgcctc tgactgattt gacaaccgac
     6661 tacctctatg agggcgcaca ggcctggtat cctcattatt catggcatag cgatggtcgc
     6721 aatatcctct acgcgggtga agttgtgcaa aaccctcccg gcacacctcc cgtcgatgtc
     6781 gcctcgttca aggcatgggg tgattttgcc gcagataaac acagtctcta ttttgaaggc
     6841 aagcgcaccg atgacaatgg tggtggaaat agcttggata tcaaaacgct gcatcaggtg
     6901 gaattccggc ctccctggga tcctgactta ctggggctga tattacgtga cgctaatttc
     6961 ttgtatatca atgggcatcg cctcgctgac cccgagagtt tccgtgtgct ggcgcagaag
     7021 tcctgggatc agcgaggaaa gttttccacc acgtttaacc cctgcattgc cgtaccattt
     7081 ggcccctggg ataccctggc tcgtacacgg accaaaatcc tgctcaacgg cgaacagctt
     7141 gatgccgacc cggacacctt ttccgtcgtg cgctggatgc ccggctcact cttgacctgg
     7201 cgtgataaaa acgggctgca gcgtaaagtc ctcgacaagg aaaatctggc gtgggatgaa
     7261 gatttaacaa agcactgtct ggatttttct ctgctggaaa agaaagtgtt ctggcgtaaa
     7321 ggacctgctt gtaaacagga agaattaccc ggactcgatc cggagcagtt tcaacccatc
     7381 agtgatgctg tcgcccagca tcaggactcg ctttatacca tcatcgaaac agagtctggt
     7441 aaccgtaagc tggagatcgt gaaacttgat gatcccaatc ttattatcaa caaacgtttc
     7501 aacgccggga aacgccacgg ctatttactt acgcgtgccg aagggtagcc ataccattcc
     7561 ggtttacacg tgtttgaatc tgacggaccg ctgatcttac tggataaccg ttctccggat
     7621 gaacgcgaag cccatcttaa tgaccatccc tttttgcgca gatggtatgc ccgcgataac
     7681 cgctacattt acagctttga tggcgcgcag ctctggcgat accgcaccgc tgatccgaaa
     7741 caagttcgct taatctggaa ggaacaacat tcgggatatg tctatggcgt aaattacaaa
     7801 acgggatatc tggacggaaa aattactgat gacggcgaat ttattcctgc cccgcgcaat
     7861 gaggcgacaa aatgaagggt caaataatca cagttgcact catcctgttg ggacacacat
     7921 ttctgacgcc tgctgtacag gccatagggt tcgactatta caatgatcac ggtgttatgt
     7981 cttacggtaa aggttattgg ggggataaga aaatcattgc ccagtttccg gagatgagca
     8041 gggccggttt gcgccttgtg aaaaatatct ctgggaaaac gggttatata ccttccgatg
     8101 aaataagcat agaaaatgag gaataccact gggtgacaga cggccgcgtt attctctggc
     8161 gtggcaaaat cgttagcaat ccaccgggaa cactcactgt cgatattgcc agctttcagg
     8221 ctatgggccg cttcgcggtc gataaatata gcctctattt tgacggacag cgcaccgaaa
     8281 gtaatagcgg cgcgtcacgt gttgagctgg cgacactaaa agctatcgaa ggtaactcta
     8341 ccacgctggt ggatagtaaa aatctctact tgtccggtcg acgtcagggg agcagcagtg
     8401 atgttactgt attagaaaaa agatggtggg gtattaatcc acgtcttatg agtgtaaaca
     8461 gaaattcata ttccaatgat ctgcttatcc gcagtgggca gaatatttat ttaaatggcg
     8521 ttcaccttac ggcgaatgca gactcatttg agataattcg ctggatacct cattcactgc
     8581 tggtttttcg cgacaataag ggtctgcatc gttatccctt tggtcaatta tcaggcaaag
     8641 cgatacccgt agatgatgac gtctcttttg aagtagggga aagtcgcgtt cgctggcgta
     8701 aacagctcac gcccgaccgt cagtggagca agtggataga cctaccaggt attgaacct
//
LOCUS       DAZSNM010000174         8664 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZSNM010000174 DAZSNM010000000
VERSION     DAZSNM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8664
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.174"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(81..272)
                     /locus_tag="ACHW42_003266"
                     /pseudo
     CDS             complement(81..272)
                     /locus_tag="ACHW42_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            270..594
                     /locus_tag="ACHW42_003267"
                     /pseudo
     CDS             270..594
                     /locus_tag="ACHW42_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(624..3536)
                     /locus_tag="ACHW42_003268"
     CDS             complement(624..3536)
                     /locus_tag="ACHW42_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFR8944959.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            4019..4861
                     /locus_tag="ACHW42_003269"
     CDS             4019..4861
                     /locus_tag="ACHW42_003269"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR8944960.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4864..5952
                     /gene="capU"
                     /locus_tag="ACHW42_003270"
     CDS             4864..5952
                     /gene="capU"
                     /locus_tag="ACHW42_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR8944961.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5957..6907
                     /gene="virK"
                     /locus_tag="ACHW42_003271"
     CDS             5957..6907
                     /gene="virK"
                     /locus_tag="ACHW42_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR8944962.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6972..7916
                     /gene="lpxM"
                     /locus_tag="ACHW42_003272"
                     /gene_synonym="msbB"
     CDS             6972..7916
                     /gene="lpxM"
                     /locus_tag="ACHW42_003272"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR8944963.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(8070..>8664)
                     /locus_tag="ACHW42_003273"
     CDS             complement(8070..>8664)
                     /locus_tag="ACHW42_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFR8944964.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT         2288 a         1968 c         2066 g         2342 t
ORIGIN      
        1 gagagtggta aaggagaggc cctggaaata ccccacagcc ccgaaaaaga gccagtcagt
       61 tgcttaactg actggcatta aggccgccgg ggacgttttt ttaccaccgg gagtttgggc
      121 ataacggtgc tttcagtaag tacgacaggt actgtggagg cacttccgta aagaagggca
      181 tcaagctcct gctccgacat acctgcaggc aaaggccatg aaagccctga tttccggaag
      241 cgagcgaaca gtccacaaac tgttgaacgt ggtgtcagcg gcggacaggg ataagccctc
      301 gtcagcagtt ccgtcagcac tgcgacagcg ttctttaccc ggtcaaaaca gcgttgcggt
      361 gcaccacgcc tgacggatga actgcgtgct cagggttacc actttaacgt aaaaaccgtg
      421 gcggcaagcc tgcgccgtca ggggctgagg gcaaaggcct cccggaagtt cagcccggtc
      481 agttaccgcg cacatggcct gcccgtgtca gaaaatctgc tggagcagga tttttacgcc
      541 agtggtccga accagaagtg ggcgggagac atcacgtact tacgtacaga tgaagttcgg
      601 ttacatcctg tcagcacgga acctcacgct ttttgagcgg cacgacggat cccccccacc
      661 cagtatccga cagacttttc actgcgttcg taaaaaccct tcttccctcc cagcatatcc
      721 actaattccg gtgacatatc ctgcagataa gtctgcaaat catgccccag atcttccggt
      781 gactgcaact cataaaccgc gcgccacaca tcacccttcc ttattttaag ccccgggtta
      841 agaatatact gtccgtggtt cagcctcaga aaaagctttt ttccataagg atttttgctg
      901 ttaacttcat gttgagagag ctggctgctg atataactcc ttcgtttgcg gcgttccggc
      961 agcaacgctt caggaagatg aaccacacac tctgccagtc gtgccgcact gaaggcttcc
     1021 gaaaaaaaca gagcacttcc cagatgcgtg tggaacagcg cctgcatcag gatcaccaga
     1081 aacattgcca tctgccggtt gtccagtttg atgaggcgtc cttccacctg cagtgacaga
     1141 ctttcaggtt tcagcagggt atacaactgc gcacttttct gttgcgcata acggggcgta
     1201 ctgaccgccg ctgacagaac ttgatgataa gccgccagcc cgtcattacc ggtcagcaag
     1261 ggatctgcac cacgctccag cagtaactga accagtgcaa tattacctgc ccgggctgct
     1321 gacatcagcg gagtaagatt aaacggcgaa cggtgatcaa caccataacg ttcaatatcc
     1381 cgcaacacgg caaccgggtt attcattgag tatggccgga aatacttcag ctccagtaac
     1441 tgcagagtct tcgttctggg ctgccttgtg ggcttaaaat cttcccggta cagtgcatta
     1501 tacagcggga acagggaata aatcagactg tactccagcg cctgcagttg tatggtttta
     1561 tcggctgtac ctgcgaggat atgctgtttc cactgtcgtg catcctctgc ggtgataatc
     1621 tgccatggca tctcagatgt ctgcaggatc cggctacgga tggtgtccgc ctgctcctgc
     1681 ttcccctgtt tttcaagcag atgtgcggtt ttctgccact catcacggct ggaaatctct
     1741 tcctgcagat tcagaggctc ttcctgatgc gtgagcgcaa gcagactcca cagaggatgc
     1801 tccacctgct gttcaacaag atacacattg tgtttagcac gggtgagcgc aacatacagc
     1861 gcgttggtaa aaaatttata gatttccgct gaacgatcct gcctgtcccg cggacgggaa
     1921 taacgggcct cgccctcaag atcagccggt gtaagaccct cgcaaatatc atcaaatgcc
     1981 tgccgggcag cgcttactat attgtaaagg atcaccgttt cgtattccag ccccttaacc
     2041 tcctgaaccg aaaagaccag cggcgtactg aaccagcatc gcgcctgtgc tttctgttcc
     2101 ggatgcatca caatgaccgc tacacgattc gaaagtgagg ttttggcatt cagttcctgc
     2161 tttgtctctt ccctatcgtc cagcaaccgg atggtccctt ctgcctgacc gcagcttctg
     2221 acaaaatggt gactttcccg atcaatagca ctaaagcgga cctgtttgag tctcagcaac
     2281 cggttagcca gtgcagtgac gtggtgcccg ttacggtaat tcgcctgcag gatattaacg
     2341 gttgtgtcgt ttccctgttg ctgccggaaa aagagacttt tgagcgaact ccatgagaaa
     2401 aagctcggat gaactatctg attggcatca ccacagagta aaaactgacc gggatgacgt
     2461 agcgttttca gtaccagttg cagttgaaca ggcgtcatat cctgaacttc atcaacaaaa
     2521 atgacatcat aacggggagt tgccaacgaa agatactgat gactgagcag attactgtca
     2581 tactgatgcg actgtttcag ccatgcgata taacgttcaa acaggacata gaccgtcggg
     2641 cggtcctcca tgccatagag cgactgccgt atccccagtg acagatacgc ctcccgggac
     2701 agcggcccat tcccggaggc aaccgcaccg atcacgcccc tgaactcctc atacagggta
     2761 tgggcagccc ccagtgcggc gatagccctg ttgcggggca gccagtcgct gaacgcactg
     2821 aaagtcacct ccctgccttc cggaatgcgc agagtttcca gaaattccgt cagcgaaaga
     2881 aagtcaatat tctgtacctc actcccttcc cctgaagcgt catagagagt acgcgctttt
     2941 tccaccagaa atgaggaaag gctgagatac agtatatcac cagcagcctg cttcattttt
     3001 tccagtacca gcgccgtctt accacttccc gccgagccaa caatcaccag cggtagcggg
     3061 tgttcgtaca gtgcctgctg atcatcatca aaggacagca ttttatccag gcggtggaaa
     3121 cgcccatgag acggattgat atacgtcagc gcttcagtcg cgatatcaac aggatccggc
     3181 actggttgtt gttgtaagcg cccttcatca atcaccacgt ttctgcgaag aaaacgtgaa
     3241 gtgttatagg catgattgcg aatatattcc agaacaaggc agatagtttc accgtggtag
     3301 cgatacagcg aaaacagtaa tcggtcccgg atactgagcc gggcgcggta aagattggtg
     3361 tcaattttac gaacatcagc ctgaacaaag ttatcggtgg caatagcctg tgcaaatttt
     3421 ttataacccg ggatcgtgtc aggattcaga gtatgataaa gacagatttt catggctgcc
     3481 ggtttccgtc gtgcaaaaaa catgctgatt ttacgaagga atgccttact gaacaacctt
     3541 gcccggaata ccccccgaaa tcagatggaa caatcagaaa aatgctctgc agttatggaa
     3601 taaagaaggt ggggtggtac gccgggaggg cgatccgctg gaaatggcgc agtagctgca
     3661 ggcctgtcat gatgcaggaa tagaagttcg tgtacaggtt aatgaaagcg tcaccccgta
     3721 aataaactct gcgctgaaat tccttttttt cattggagta cctgtgttgt tctgaggtga
     3781 acatatcacc tctgttcagg tagccaaatt cagtgtgcca cctcaaggtt cttagtgagt
     3841 cgtttttaaa agatatgata atatatcatt gtttatgaat tgttgatgtt taatttagta
     3901 aattatatca aatatgccat gggtttgcta tgttattcat ccagtatctg gaaataaaat
     3961 gcaccagctt tattgggttt tccttttgtc tctgcttgct aggtgccaga aactttaaat
     4021 gttaaacgaa gggggcattt tgttcaaagc caatcatgtt ccggtattaa tgtatcatca
     4081 tgtcagtcat tgccccggac tggttacact ttctcccgtg acattccgga agcaaataaa
     4141 atggttggct gaaaacaact ggaaaacact gtcatcagat gaactggagt ttttttatcg
     4201 gggcggaaaa ctcccccgga aaagcgttat gttgacattt gatgatggat accttgacaa
     4261 ctggtttcag gtatatcctt tattaaaaga gtttaatctc aaagcacata tttttttaat
     4321 taccggtttt attgggaatg gtccggttcg tcactctcct ggaaaggaat attctcatcg
     4381 cgattgcgaa catcaaatag cgacagggaa tgctgataat gttatgcttc gctggtctga
     4441 agtaaacgaa atgttgcaat ctggtctggt cgaatttcat gttcatacgc atactcatac
     4501 acgctgggat aaaaagttca gttcacgaga agaacagtgt aaacatcttc ggcaggactt
     4561 gttgagcggg agggaatatt tgaaagaaat gacaggaaaa tgcagtaaac atttgtgttg
     4621 gccggaaggt tattataaca aggattatat tcaggtcgct gaagaacttg gattctatta
     4681 tttatataca acagaaagaa gaatgaatgc tcctgctaaa ggaactacac ggataggaag
     4741 aataagcacc aaagaacggg aaagttgcgc atggctgaaa agacgacttt tttattatac
     4801 aaccccgttt ttttcatcgt tgctggcctt tcataagggg ccaagattac cggatgatta
     4861 ataatgaata tactatttac ggaatcatca ccgaatatag gtggtcagga attacaggct
     4921 gttgctcaaa tgaaggccct gaagaaaatg gggcattcag ttctgcttgt ctgcagggaa
     4981 aacagcaaaa ttgcttttga agccagtaaa ttggggattg atatcacatt cgcgttattt
     5041 cgaaacagtc ttcacatccc tactgcatgg agattactcg gaatagttca tggttttcag
     5101 cccaatgcaa tcgtttgtca cagtgggcat gatagcaata ttgttggttt agtacggtta
     5161 ttcactagga aacatccatt cagaattatc aggcaaaaga catatttgac acgaaaaaca
     5221 aaagtttttt caataaatca tttttgcgat gaggtgattg ttcccggaac aagtatgaag
     5281 acacatctgg agcaggaagg atgtcgaacc cgggttactg ttgtgcctcc aggctttgat
     5341 ttccagaaat tatacgttga ttcccgaaac agtttgcctc caaatgttct ttcttggctg
     5401 gcgtcccgaa ggggatgccc cgttattgct caggtaggaa tgttgcgccc ggaaaaaggg
     5461 cacgaattta tgttgaattt actgttccat ttaaaaatga atggacgaca gttctgttgg
     5521 ttgattgtgg ggtctggttc gcctgaactg cgggagcatt tacagtatca gattgacagt
     5581 atgggcatgc atgatgatgt ttttattgct gacaatgttt ttcctgccgc ccccgtatat
     5641 cgggttgcca gtctggtggt tctgccttca gaaaacgaat cgtttggtat ggtactggca
     5701 gaagcatcgg cattttctgt gcctgtactg gccagtcaga ttggtggaat ccctgatgtt
     5761 attcagaata accagaccgg gacattgtta ccagcaggta ataagcacgc atggatgtgc
     5821 gccctgaatg atttttttaa tgaccctggg cgtttttatc agatggctcg ccaggcaaaa
     5881 caggatatag aagagcggtt tgatattaat aaaactgcgt taaaaatact cacattagcg
     5941 aagcacaagt aacaatatgt tttctgtaag taacttatca tttatcggtt tccttaaaag
     6001 gattgttttt tcctcagatt cactcccggg gaagtgggaa cacagaaaat tccggttcat
     6061 gtacattttg cgatgtgcta taaatccggt tgccagtatt cgatattatt acgaactgcg
     6121 ttccttgcag tgcattgagg atattctggc aatacaaccc acgttgccag caagaattca
     6181 tcgaccttat ttgcataaag gggggcgagc ctggtcccgg gggcaatata ttcttgagca
     6241 ttatcgcttt gtgcaaaatt tgccagaaaa atattctgaa tttttgtttc cacaaaaatc
     6301 ggtatcatta gtccaattca tcgggaaaga tggagaggat tttgatatcc agtgttctcc
     6361 cagtggtttt gaccgggaag gagagttgat gttgtcattg tttttcaata aaatagtgat
     6421 tgcccggtta acattttcag tcattctgac tcaaaatgga cacaccgctt ttattggcgg
     6481 attacaggga gcccccaaaa ataccggacc tgatattatt cggtgcgcaa cccgggcctg
     6541 ttatgggctt ttcccaaaac ggattatttt tgaagcattt tgtgcgttga tgaaagcttg
     6601 taatgtctct gagtgtctgg cagtcagtga gcacagtcat gttttccggc aattgagata
     6661 ctggtatcag aaacgcaaaa cctttgttgc tgtctacagt gatttctggg agtctgtagc
     6721 agggaaaacc tgtggtgact ggtacaaatt accaacacag gtggttcgaa agccgctaag
     6781 caatatagct agtaaaaaac gctctgagta tcgaaaacga tacgctttgc tggattatat
     6841 ccatgaaact gctatccgct ctttggatgc atatcctgta aactcagaac atcaggactt
     6901 aaattaaggt ttgatgtatt ctctgaataa aatattaatg atgattttgg taggggcatt
     6961 cgcactaaat aatgaaaaaa tacaaatccg agtttattcc tgaatttaag aaaaattatc
     7021 tttcccctgt ttactggttt acatggttcg ttttgggaat gattgcaggt atttcaatgt
     7081 ttcccccttc attcagagat cctgtcttgg ccaaaatagg gcgttgggtg ggcagattga
     7141 gcagaaaagc tcgtcgcagg gcgacgatta atttatcgct ttgtttcccg gaaaagagtg
     7201 atacagaacg ggaaataatt gtcgacaata tgtttgccac agcattgcaa tccatagtga
     7261 tgatggcaga actggcaatt cgtggtccgg aaaagttcca gaaacgagtg ttctggaaag
     7321 gcctcgaaat tcttgaggaa attcggcaca ataacagaaa tgtgattttt ctggttcccc
     7381 atggctggag cgtggatatt cctgcaatgt tgctggcagc tcagggggaa aaaatggccg
     7441 ccatgtttca tcagcaacga aatccagtga ttgattatgt ctggaattct gtacggcgta
     7501 aattcggggg gcgcttacat tcccgggagg atgggataaa accatttatt cagtcagtac
     7561 gccagggata ctgggggtat taccttccag atcaggatca tggtcctgaa tacagtgaat
     7621 ttgctgattt ttttgcgacc tataaagcga cattaccaat tattggacgt ctgatgaaca
     7681 tcagtcaggc tatgattata ccgcttttcc cggtttatga tgaaaaaaaa catttcctga
     7741 ctattgaagt tcggccacca atggatgcat gcattgccag cgcggacaat aaaatgattg
     7801 cccgacaaat gaacaaaaca gtggaaattt tggtggggtc acatccggaa cagtatatct
     7861 gggttttaaa attgttaaaa acgcgcaaat caaacgaagc ggacccgtac ccttgaaatt
     7921 ataaagtaca ggtatttcca ctagttgttt cttacaggtt accaatcgaa acacatcccc
     7981 cttccgtgtg ttgcccgttt ggcacggagg actgtgtaga tgcagttcgt taaatttaat
     8041 ttatgcctaa atagctgcgc ttaataccgc tacacttttg ccagcccatg tttgcctccg
     8101 gggaatgggc tgacggtttc cataaaatgg cgaacttttt tcaacagttg ccacattgag
     8161 cggcactgat gattacgcgt tattgtgtcg tgaagtgcct gccatagccg ttcaacatga
     8221 ttcacccatg gcgagtaaac cggctgataa atgcccctga acttcgggtt ctccttcagc
     8281 cagctctgtg tttcccggct tttgtggata atgtagttgt ccacgatcag cgtgatggtt
     8341 ttcgcacgac ggtatgtcgc tttaagccgc ttcagcaggc tgatgaacag cgccgaactt
     8401 ttgctgttgc cgcccacata gctgacttta cctgtcccgc tgtgcagcgc tccggccaga
     8461 taatattttt cattctgtcc cggcgtgacc acccgttttt gctgtccgcg cagttgccag
     8521 tccgcaccga ttttgggatg aagatggata tccacttcat cttcataaaa gaccggatgc
     8581 tctgcgctgc attcgtccag tgctttatgg attgctgcca tcttttcatc tttatgcggg
     8641 tcacggatac gcagagttgg cgca
//
LOCUS       DAZSNM010000175         8518 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZSNM010000175 DAZSNM010000000
VERSION     DAZSNM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8518
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.175"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..265
                     /gene="csgC"
                     /locus_tag="ACHW42_003274"
     CDS             <1..265
                     /gene="csgC"
                     /locus_tag="ACHW42_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR8944965.1"
                     /translation="LEHLCCSEVSISPLFRWPNSVCHFKGSSGQSQTKQEKTLSLPAN
                     QPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQWTPSSEKS"
     gene            386..697
                     /gene="ymdA"
                     /locus_tag="ACHW42_003275"
     CDS             386..697
                     /gene="ymdA"
                     /locus_tag="ACHW42_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR8944966.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            792..1325
                     /gene="ymdB"
                     /locus_tag="ACHW42_003276"
     CDS             792..1325
                     /gene="ymdB"
                     /locus_tag="ACHW42_003276"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR8944967.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1267..2748
                     /gene="clsC"
                     /locus_tag="ACHW42_003277"
     CDS             1267..2748
                     /gene="clsC"
                     /locus_tag="ACHW42_003277"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR8944968.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2756..3913)
                     /gene="mdoC"
                     /locus_tag="ACHW42_003278"
     CDS             complement(2756..3913)
                     /gene="mdoC"
                     /locus_tag="ACHW42_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR8944969.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4307..5842
                     /gene="mdoG"
                     /locus_tag="ACHW42_003279"
     CDS             4307..5842
                     /gene="mdoG"
                     /locus_tag="ACHW42_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR8944970.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPLPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5835..8378
                     /gene="mdoH"
                     /locus_tag="ACHW42_003280"
     CDS             5835..8378
                     /gene="mdoH"
                     /locus_tag="ACHW42_003280"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR8944971.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2153 a         2016 c         2220 g         2129 t
ORIGIN      
        1 attggagcac ctgtgttgtt ctgaggtgag catatcacct ctgttcaggt ggccaaattc
       61 agtgtgccac ttcaaaggca gttcagggca aagtcagacg aagcaagaaa agaccctctc
      121 attgcctgct aatcaaccca ttgctttgac gaagttgagt ttaaatattt ccccggaaga
      181 tcgggtgaaa atagttgtta ctgtttctga tggacaggca cttcatttat cacaacaatg
      241 gacgccctct tcagaaaagt cttaatttgt tgaaatatcg agcataagat gaatctggag
      301 agaatggtct gctgcgaatc agccaacctg aaagtatgga taacacaacc ttcaaggatg
      361 actaatcatt gaggaaatag aataaatgtt cagacctttt ttagactctc ttatgctcgg
      421 cagtttgttt tttcctttta ttgccattgc aggaagcacc gcgcaagggg gcgtgatcca
      481 tttttatggc caaattgtgg aaccggcatg tgacgtcagc acccagtcat cacccgtaga
      541 aatgaactgc ccacagaatg gttctattcc gggcaaaacc tactccagca aagcgttaat
      601 gagcggcaat gtcaaaaacg cgcaaatagc ctcagtaaaa gtgcagtatc ttgataaaca
      661 gaaaaagctg gctgtaatga acatcgaata taactaagtt tctgaacaac tcacgttgct
      721 gagcagaaaa atgcgattta accaaaaagc ctgctgtaca cttaagaaac aagaaggtgt
      781 aaggaggcgt tatgaaaacg cgtattcatg ttgtgcaggg tgatattacc aaactggccg
      841 ttgatgtgat tgtgaatgcg gctaatccgt cattaatggg aggcggcggc gtcgatgggg
      901 ccattcatcg cgcagcgggt ccggccctgc tggatgcctg tttaaaagtc aggcaacagc
      961 agggcgattg ccctacgggg catgccgtta ttacgcttgc aggcgatctt cccgctaaag
     1021 ccgtagtaca caccgtcggg ccagtctggc gtggtgggga acaaaacgaa gaccagcttt
     1081 tgcaggatgc ctatctgaat agcctacgac tggtggcggc aaacagctat acgtcagtgg
     1141 cttttcctgc aatcagtact ggggtttata gttaccctcg tgcggcagcg gctgaaatcg
     1201 cagttaaaac cgtttcagaa tttattaccc gtcacgcttt acccgaacag gtatactttg
     1261 tctgttatga tgaagaaaac gcccacctct acgaaagact ccttacccaa caaggagatg
     1321 aatgatttgc cccggctggc gagcgcggtg ctgccactgt gttcgcaaca tcccggtcag
     1381 tgtggtcttt tccctctgga gaaaagtctg gatgcgtttg ccgcccggta tcgtctggcc
     1441 gaaatggcag agcatacgct ggatgttcag tattacatct ggcaggacga tatgtcgggg
     1501 cggttactgt tttccgccct gttagccgca gcaaagcgtg gcgttcgcgt ccgtttgttg
     1561 ctggacgaca acaatacgcc tggacttgac gacattttgc gcttgcttga cagtcatcca
     1621 cgcattgaag tccggctttt taatcctttc tcgtttcgct tgctacgtcc gcttggatat
     1681 atcaccgact tttcccgtct caatcgccgg atgcacaata aaagtttcac tgtcgatggc
     1741 gtggttaccc tagtgggagg acgaaatatt ggtgatgcct attttggtgc aggggaagag
     1801 ccactttttt cggatttaga tgtcatggca ataggccccg tggtagagga tgttgccgat
     1861 gatttcgccc gctactggta ttgcaaatcg gtttcaccct tacagcaggt gctggatgtc
     1921 ccggaaggtg aaatggcgga tcgcatcgag ttacccgcct cctggcataa cgatgccatg
     1981 acgcatcgtt atttacgcaa aatggaatcc agtccattta taaatcatct ggttgatgga
     2041 acgttgccgc ttatctgggc gaagacacgt ttattaagtg atgatccggc gaaaggggag
     2101 ggcaaggcaa aacggcattc actgttaccg cagcgcctgt tcgatatcat gggctcaccc
     2161 agtgaacgca tcgatattat ctcttcctat tttgtaccga cacgcgcagg tgtggcgcaa
     2221 ctcttacgga tggtgagaaa aggggtaaag attgcgatcc taaccaattc tcttgccgct
     2281 aacgatgttg ctgtcgtcca tgccggatac gcgcgctggc gcaaaaaatt gctccgctat
     2341 ggcgtggaat tatatgaact caagccgacg cgtgaacaaa gcagtacgtt acacgatcgc
     2401 ggcataaccg gtaattccgg tgccagcctg catgctaaaa cttttagcat cgatggtaaa
     2461 acggtgttta tcggctcttt caatttcgat ccgcgttcaa cattgctcaa tactgaaatg
     2521 ggcttcgtga tagagagcga aacgctggca cagttaattg ataaacgctt tattcagagc
     2581 cagtacgatg cggcctggca gctccgtctg gacaggtggg gacggatcaa ctgggttgat
     2641 cgtcatgcaa agaaagaggt tattctcaaa aaagaacccg ccaccagttt ctggaagcgg
     2701 gttatggtca gactggcgtc gatattgccc gtggaatggt tattgtaaat gtggcttaac
     2761 gggctggtgc tttatcgttc tcacgcttaa caaccggttt accagagaac aaaaacttca
     2821 gtaacgggat gcgtaaatga atttcataca gaattatcgc aatccctact acgaatatca
     2881 ggccacagag aaaaccaagc cagttggagg tgatgtgcgg tgtaatgtat gcgccgaaaa
     2941 acagcgttaa cgggtggtga accagataga taaacagcga tgcgttaaca aaataagtca
     3001 cccgcgctga ctggaagtta agcaaacggt ggccaaagga gaagaccaca ttcaccatcc
     3061 acaggccgag gaccatggtg atcaccgact cggtttcgta catccaggca tcgccactgc
     3121 catagcgctg gttgagtaaa taagcgacaa acgccaatgc tgcagcgagg gtacagccac
     3181 gagacggcgt ggtaaacaag gctttaagat gagggaaaat gaaagccagt gcgccgagga
     3241 taaagaacgg taaataaaat agcgtttgca tgacaataaa attgaacatg ccattactca
     3301 gaatgggtgg atacacaata aaaatcgttc ttcttattac cgcatagccg atgccgaggc
     3361 ataaaaaaat caccgatagt tttaccatcg agaatttttt attcgtttta tcagaatttt
     3421 ctaaattatt tctgatgcgc ttaaatatcc atacacacag cgtcgtcatg actactaaca
     3481 ccagtaaaaa ccacaggtgt gatattaatt cccaggccaa cgtattatat ttgtcataca
     3541 atgatagccc aggccaactt tccgctttcc ctttgacata ttgcagcata ataaattgcg
     3601 gtaatgtcag tagggggatg gctgttaaca tcgggatacc tacacgttcg acacgtactt
     3661 tccaccattt tttcaaggga tagcgtaaaa aaagcatgta ggaaaagtat ccggatataa
     3721 cgaaaaatac ctgcatgcgg aacgagtgga tgaagtcatt aaaaagggtt agccacaatg
     3781 acggttcggc gctattcaca tgccatgtat ggctcgaata gattaaagaa atatgaaaag
     3841 ggatccctaa caacatcagc caggcgcgga tggagtcgag gaaatattca cgttgcgcgg
     3901 gtactggatt catatatggt taactaatct cggatttttc gtcttatccc tgtcgggtta
     3961 tgcctttagg cttgttgcca tagcgacacc gacctgaccg cgccaggcgc aggcttcaag
     4021 gtttttatgc atagcatcat cgctaccact aaccagaatg gaagcgtctg taagacggtt
     4081 gataaataaa tttgctggca aaccctacac gaagtcgatg cttctgtctt taggagaagc
     4141 acggaaagtg aaaacggttg caatcaggtg cttaatccat gagccagcgt gctgaacgat
     4201 accgggattc agttgtcgga atggctggtt atccattaaa atagatcgga tcgatataag
     4261 cacacaaagg gggaagtgct tactaattat gaaacataaa ctacaaatga tgaaaatgcg
     4321 ttggttgagt gctgcagtaa tgttaaccct gtatacatct tcaagctggg ctttcagtat
     4381 tgatgatgtc gcaaagcaag ctcaatcctt agccgggaaa ggctatgagg cgcctaaaag
     4441 caacttgccc tccgttttcc gcgacatgaa atacgcggac tatcagcaga tccagtttaa
     4501 tcatgacaaa gcgtactgga acaatctgaa gaccccattc aaactcgagt tctaccatca
     4561 gggtatgtac ttcgataccc cggtcaaaat aaatgaagtg actgccaccg cagtcaaacg
     4621 aatcaaatac agcccggatt atttcacttt cggcgatgtt cagcatgaca aagacacggt
     4681 aaaagacctt ggttttgccg gttttaaagt gctttacccg atcaacagca aagataaaaa
     4741 cgatgaaatc gtcagcatgc tcggggccag ctatttccgc gtgattggtg caggtcaggt
     4801 ttatggcctt tctgcccgcg gcctagcaat tgataccgct ttgccatcgg gtgaagaatt
     4861 tccacgcttc aaagagttct ggatcgagcg tccaaaaccg actgataaac gtttaaccat
     4921 ttatgcattg cttgactcgc cgcgtgcgac aggtgcttac aaattcgtgg ttatgccagg
     4981 gcgtgacacg gttgtggatg tgcagtcgaa aatctatctg cgcgataaag tcggcaaact
     5041 gggggttgca ccgttaacca gtatgttcct gtttgggccg aaccaaccgt tgcctgcaaa
     5101 taactatcgt ccggagttgc acgactctaa cggtctgtct atccatgctg gtaatggcga
     5161 atggatctgg cgtccgttga ataacccgaa acatttagcg gtcagcagct tctccatgga
     5221 aaacccgcga ggctttggtc tgttgcaacg aggtcgtgat ttctcccgct ttgaagatct
     5281 cgatgatcgt tacgatctcc gtccaagcgc atgggtgact ccgaaagggg agtggggcaa
     5341 aggcagcgtt gagctggtgg aaattccaac caacgatgaa accaacgata acatcgtcgc
     5401 ttactggacg ccggatcagc tgccggagcc gggtaaagag atgaacttta aatacaccat
     5461 caccttcagc cgtgatgaag acaaactgca tgcgccagat aacgcatggg tgcaacaaac
     5521 gcgtcgttca acgggggatg tgaagcagtc gaacctgatt cgccagcctg acggtactat
     5581 cgcctttgtg gtcgatttta ccggcgcaga gatgaaaaaa ctgccagagg ataccccggt
     5641 cacagcgcaa accagcattg gtgataatgg tgagatagtt gaaagcacgg tgcgctataa
     5701 cccggttacc aaaggctggc gtctggtgat gcgtgtgaaa gtgaaagatg ccaagaaaat
     5761 cactgaaatg cgtgctgcgc tggtgaatgc cgatcagacg ttgagtgaaa cctggagcta
     5821 ccagttacct gccaatgaat aagacaactg agtacattga cgcaatgccc atcgccgcaa
     5881 gcgagaaagc agcattgccg aagactgata tccgcgccgt tcatcaggcg ctggatgccg
     5941 aacaccgcac ctgggcgcgg gaggatgatt ccccgcaagg ctcggtaaag gcgcgtctgg
     6001 aacaagcctg gccagattca cttgctgatg gacagttaat taaagacgac gaagggcgcg
     6061 atcagcttaa ggcgatgcca gaagcaaaac gctcctcgat gtttcccgac ccgtggcgta
     6121 ccaacccggt aggccgtttc tgggatcgcc tgcgtggacg cgatgtcacg ccgcgctatc
     6181 tggctcgttt gaccaaagaa gagcaggaga gcgagcaaaa gtggcgtacc gttggtacca
     6241 tccgccgtta cattctgttg atcctgacgc tcgcgcaaac tgttgtcgcg acctggtata
     6301 tgaagaccat tcttccttat cagggttggg cgctgattaa tcctatggat atggttggtc
     6361 aggatttgtg ggtttccttt atgcagcttc tgccttatat gctgcaaacc ggtatcctga
     6421 tcctttttgc ggtactgttc tgttgggtgt ccgccggatt ctggacggcg ttgatgggct
     6481 tcctgcaact gcttattggt cgcgataaat acagtatatc tgcgtcaaca gttggcgatg
     6541 aaccattaaa cccggagcat cgcacggcgt tgatcatgcc tatctgtaac gaagacgtga
     6601 accgtgtttt tgctggcttg cgtgcaacgt gggaatcagt aaaagccacc gggaatgcca
     6661 aacactttga tgtctacatt ctgagtgaca gttataaccc ggatatctgc gtcgcagagc
     6721 aaaaagcctg gatggagctt atcgctgaag tcggtggcga aggtcagatt ttctatcgcc
     6781 gccgccgtcg ccgcgtgaag cgtaaaagcg gtaatatcga tgacttctgc cgtcgctggg
     6841 gcagccagta cagctacatg gtggtgctgg atgctgactc ggtaatgacc ggtgattgtt
     6901 tatgcggcct ggtgcgcttg atggaagcca acccgaacgc cgggatcatt cagtcgtcgc
     6961 cgaaagcgtc cggtatggat acgctgtatg cgcgctgtca gcagttcgcg acccgcgtgt
     7021 atgggccact gtttacagcc ggtttgcact tctggcaact tggcgagtcg cactactggg
     7081 gacataacgc gattatccgc gtgaaaccat ttatcgagca ctgcgcactg gctccgctgc
     7141 cgggcgaagg ttcctttgcc ggttcaatcc tgtcacatga cttcgtagaa gcggcgttga
     7201 tgcgccgtgc aggttggggg gtctggattg cttacgatct cccgggttct tatgaagaat
     7261 tgccgcctaa cttgcttgat gagctaaaac gtgaccgccg ctggtgccac ggtaacctga
     7321 tgaacttccg tctgttcctg gttaagggta tgcacccggt tcaccgtgcg gtgttcctga
     7381 cgggcgtgat gtcttatctc tccgctccgc tgtggtttat gttcctcgcg ctctctactg
     7441 cattgcaggt agtgcatgcg ttgaccgaac cgcaatactt cctgcaacca cggcagttgt
     7501 tcccggtgtg gccgcagtgg cgtcctgagc tggcgattgc actttttgct tcgaccatgg
     7561 tgctgttgtt cctgccgaag ttattgagca ttttgcttat ctggtgcaaa ggaacgaaag
     7621 aatacggcgg cttctggcgc gttacattat cgttgctgct ggaagtgctg ttttccgtgc
     7681 tgctggctcc ggtacgcatg ctgttccata cggtcttcgt tgtcagcgcg ttccttggct
     7741 gggaagtggt gtggaattca ccgcagcgtg atgatgactc cacttcatgg ggcgaagcgt
     7801 tcaaacgcca cggctcacag ctgctgttgg ggttagtgtg ggctgttggg atggcgtggc
     7861 tggatctgcg tttcctgttc tggctggcac cgattgtctt ctcgttgatc ctgtcaccgt
     7921 ttgtttcggt gatttccagc cgtgccaccg ttggtctgcg caccaaacgc tggaaactgt
     7981 tcctgatccc ggaagagtat tcaccgccgc aggtgctggt tgataccgat cggttccttg
     8041 agatgaatcg ccaacgctcc cttgacgatg gctttatgca cgcagtgttt aacccgtcat
     8101 ttaacgctct ggcaaccgca atggcgacag cgcgtcaccg cgccagtaag gtgctggaaa
     8161 tcgcccgtga ccgccacgtt gaacaggcgc ttaacgagac gccagagaag ctgaatcgcg
     8221 atcgtcgcct ggtgctgcta agcgatccgg tgacgatggc ccgtctgcat ttccgcgtct
     8281 ggaattcccc tgaaagatat tcttcatggg tgagttatta cgaagggata aagctcaatc
     8341 cactggcatt gcgtaaaccg gatgcggctt cgcaataaaa acgtagttgc ctgatgcgct
     8401 acgcttatca ggcctacatc gttcctgcaa tttattgatt ttgcaagatt ttgtaggtcg
     8461 gataaggcgt tcacgccaca tccggcaaaa acaacgagcc aataaaaata ccggcgtt
//
LOCUS       DAZSNM010000176         8448 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZSNM010000176 DAZSNM010000000
VERSION     DAZSNM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8448
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.176"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            44..397
                     /locus_tag="ACHW42_003281"
     CDS             44..397
                     /locus_tag="ACHW42_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR8944972.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(434..2512)
                     /gene="flhA"
                     /locus_tag="ACHW42_003282"
     CDS             complement(434..2512)
                     /gene="flhA"
                     /locus_tag="ACHW42_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFR8944973.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="ACHW42_003283"
     CDS             complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="ACHW42_003283"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR8944974.1"
                     /translation="MNNIQLAHGSGSQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="ACHW42_003284"
     CDS             complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="ACHW42_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR8944975.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="ACHW42_003285"
     CDS             complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="ACHW42_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR8944976.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="ACHW42_003286"
     CDS             complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="ACHW42_003286"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR8944977.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="ACHW42_003287"
     CDS             complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="ACHW42_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR8944978.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6415..6876
                     /gene="hycA"
                     /locus_tag="ACHW42_003288"
     CDS             6415..6876
                     /gene="hycA"
                     /locus_tag="ACHW42_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR8944979.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7001..7612
                     /gene="hycB"
                     /locus_tag="ACHW42_003289"
     CDS             7001..7612
                     /gene="hycB"
                     /locus_tag="ACHW42_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR8944980.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7609..8448
                     /locus_tag="ACHW42_003290"
                     /pseudo
     CDS             7609..8448
                     /locus_tag="ACHW42_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
BASE COUNT         1891 a         2289 c         2278 g         1990 t
ORIGIN      
        1 attaagatgt aatataaatg catcttaatg aggtggaggc agcatgtccg gcaagcgtat
       61 ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca
      121 ggcgtcagcg gagccggagc attacgaggc gttgtttgtt tacgcgcaaa agcggctgga
      181 taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca
      241 gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg
      301 gcgtcatccg atcttaaccg tgcgtcatct catcgatgac aagcgtccgg tgccagaact
      361 gccggaaaaa tatcgtccga aaaaacctcg tgaatagtat gccggacagg cagatctgtc
      421 cggcaatttg cagttaaatc aatgccgatt tatcaattcc cagccgcttc atccgtgaca
      481 gcagggtcgt gcgtttcagc cccagacgtt gcgcagcgcc tttaggcccg gcaaccacgc
      541 tgttggtttc tttcagcacg cgcacaatca actgatattc atcttcgccc tcctgggcga
      601 caaccgttgc ggcaggcggc gtttcaggct ccggtaaagc aatatctggc aatgacagct
      661 gcagcacgtt gccgcgtgtt agcaatactg cgcgctcaat gacgttttcc agttcgcgta
      721 cgttacccgg ccactccatg ttgctcaagg tgcgcagcgt ctcggcagga atgctgtcga
      781 tattgcgccc cagacgacgg gcaattttga aggtaaaggc tttcgccagc agcggaatat
      841 cttccggacg ctcgcgtagt ggcggcaggt gaatcgggaa tacgttcagg cggtaataga
      901 gatcgctacg gaactctcgg tcggcgacca tttttttcag atcgcggtta gtcgcggcaa
      961 ttaaacgtac gtccgtctga atgattttgt tgctgccgag acgttcaaac tcctgttcct
     1021 gcaatacacg cagcaacttc ggctgtaact ccagtggcat atcgcccact tcgtcgagga
     1081 acagagagct tttatccgcc agttcaaaac gaccaatgcg ctgggcgttg gcaccggtaa
     1141 aagccccacg ctcatgacca aacagatcgc tttccagcaa tccggcaggc atcgccgcgc
     1201 agttcatttt gaccatgcgg cgattattac gcccactgag attatggatc gcacgggcaa
     1261 tcagctcttt acccgagcca gtttcaccga ggatcagcac ggtactgtca ctttgcgcca
     1321 ccatttcaac ttgtttaagc acgctgtaca tggcttcgct gcggccaata atctcgccaa
     1381 attcactatc aacattgttg agctgctcgg tcagggcgag gttttcatca accagccgtt
     1441 ctttcagacg atggatttcc tgataggcga gggcgttatc gacagcgatt gccacacgtt
     1501 cggcaatctg gcgcagcaaa ttcagattgg tagtggtaaa cactttctct tcgcattgcg
     1561 ccagtttcag cacgcccagc atggtgtcgc cagacatcag cggtaacagg cacaaggttt
     1621 gaatctggtt gccccaggtg tcgaacaaca tgcgttcata gggggctaaa tcgtcccgct
     1681 cgtagagatt gatcagcagc atctctttac ttttgaacac gcgttcggtg agggttccgg
     1741 cttcatcgac ttcgctctgt tcgtgggcgg gatgttgttt atcaagatag tgagtggagt
     1801 agatgttgag tttgttttta cggtggctgc gtaagacaat gctgatatcg tcaatgtcga
     1861 aatagtaatg gatttctttg gcgacttcgc tgaccagttc gtccatatcc aggcgggaaa
     1921 gcaccgcgtt ggtaatggca accaggatgc ggaagttatc gcgctcccgg cataacaact
     1981 catagtcgac attgttgacg acgcggctct ggatttgttc ggtgacgaca gaaacgatct
     2041 gcgtaaatgt ttgcagacga ttgaactctt tttcgctcca gggacgctcg tcataacgaa
     2101 taaattcaca gccaccaaaa atatgccctc ccgccgccag tggcatcagg caatagtgac
     2161 caaatttggg gtatagccca cctgcggcca gctgcggcca ggtttcacaa aattcttcgt
     2221 aactgcaatg cagcgtatca gggcgcgaca aaatgctgcg taccggaccg tgtgccagaa
     2281 cagtttcgtc ttcatattta atgggggtgt ctttttcacg cgacgcgtaa taagacgcac
     2341 gttgagtctg cgcttgccac aacacaatag ccgcgttgtc ggcgagcgca gaacgcttta
     2401 ccagttgcga aagagcctca cacagcgagg ccagatcggg ctgctgcaat agtgtccgag
     2461 tgatgtcgaa caacccttgc tgtccgagat cactcatcgg tgtatatgac attttgttct
     2521 tccaggaaag caccgcaaag cggtgcaaaa ggtcattgac taactatagg agatttagaa
     2581 ttttattagc atatacgcgg aagcggttcg gcgtgtggta aatcgagggt tcgtttcacg
     2641 ccatacagac cggcaagacg aacaccttta cgttccacca cttcaccaat cagcgccgcg
     2701 tctttcccca gtggatggga atgtaacgct gccagcactt gctctgccgc gttgcgttca
     2761 acggcgatca ccagtttgcc ttcgttggca aagttcaggg cgtccagtcc cagcaattcg
     2821 caaacgccgc gcacggcagg tttaaccggc agcgctgatt cagaaatttc aataccacaa
     2881 ccgcaggctg ccgcgaactc atgaaccacc gcgtttacac caccacgggt ggcatcacgc
     2941 agcgctttca cgccgggaat gtcacgcagc gtctgaataa gcggcgtcag caccgcgcag
     3001 tcgctgacca gttcgccatc cagccccagc tgctcacgca ggttaaggat agtcgccccg
     3061 tggtcgccga gtgtaccgct aaccagcaat acatcgcctg cggttagcgt ctgtgcgccc
     3121 cagtgaatat tcgccggaat tgcgcccatg ccagcggtgt tgataaacag tttatctgcc
     3181 gcgccgcgct gcaccacttt agtatcgcca gtaacgatgg caatgcctgc cgtgcgggcg
     3241 gtttctgcca tgctggtcac tacggctttc agtgtctcca tcggcaatcc ttcttcgagg
     3301 ataaagccac aggagagata gcgcggaata gcgccactgc ccgcaacgtc attcgcggtg
     3361 ccgcaaatcg ccagcttgcc gatattaccg ccaggaaaga acagcgggtc aatgacgtaa
     3421 ctgtcggtgg agaacgccag acggtcgcct tccgctacca gctgcgccag atcaagacgt
     3481 gcctgatctt cctgctctgc cagccacggg ttggcaaagg cttccataaa caggctgttg
     3541 attaattgct gcatcgcctg gctgccgctg ccgtgggcga gttggatatt attcacgctt
     3601 cactctcctg ctgacgatac tgataccacg cggcgcacgc tccttcggag gagaccatca
     3661 gtgcaccaaa tgcggtttga ggattacagg tgttaccaaa cagcgggcat tgatgcggct
     3721 tacatttgcc cgttaatacc tcaccacaac gcgcgcgcgg gtcatcgcag acctgctgcg
     3781 gtgccgggcg gaaatgtgct tcggcatcga atcgttgata atccggcgtc aggtgcacgc
     3841 cagaagattc aatcacgcct aagccgcgcc attcgctgtc gccgttgaca cagaacacat
     3901 cggcaatcgc ctgttgcgcc agtaggttac cggcatccgg taccacccga cgatactgat
     3961 tctctacctt gctgtgggcc gctattttct gctccaccag catgaccacg ccttgcagta
     4021 gatcaagggg ttcgaaacca gcgaccacca gcggacgatg aaaatcgctg gcgataaaat
     4081 tataggcatc agtgccgata accatactaa cgtggcccgg cgcgaggaac gcgtcgatac
     4141 cgttatccgg ctgttccagc aaactgcgca gcgtcgggat aagcgtaatg tgctggcaga
     4201 agaagtaaaa attctgcaca tcacgcgctt tcgcctgttg cagagtgatg gcggtggtcg
     4261 gcatggtggt ttcaaaacct aagccgaaga acaccacttt gcgggttgga ttctcctgcg
     4321 ccagtttcaa cgcatccatc ggcgagtaaa cgatgcgcac atcggcaccg cgtgcttttg
     4381 cctgcaacag cgatccctgt ttccctggta cgcgcatggc gtcgccaaag gtacagaaga
     4441 tgacttccgg atggctggca atctccacgc aggtgtcgat tctacccatc ggcagtacgc
     4501 acaccgggca acccggaccg tggataaact caacgttttc aggcagtaac tggtcgaggc
     4561 cgaatttaaa aatggcgtgg gtatgaccgc cacacacttc cataatccgc agagggcgtt
     4621 cggcggtgta agagagatgt gaagcacgtt cgcgcagatg ctcaattaac tgcatcacct
     4681 gttccggcgc gcgatattca tcaacaaaac gcattatttt tcctcgccat acaacagcgc
     4741 gccgacatcc ggctcaacgt caaacatgtt ttgtaaggcg tcgagagtgt cgcgtgcttc
     4801 ggcttcatta attacgctca tggcaaagcc aacgtgaacc agtacccact ggcccacgcg
     4861 cggctgaccg ttttcatcgc agctgccgac taacgttaaa tcgacatcgc gctgaatgcc
     4921 gcagacgtcg actttcgcct ggttgccgtc aatggtgcgg atctggccgg gaacgcctat
     4981 gcacatcgct gtgtctccag ccagttcagc cactggtcca tcccttcgcc gctggtggcg
     5041 gaaataagga tgatttcaat ttctggattg acttcgcggg cgcaggcgat gcacttctca
     5101 acgtcaaagt tgagatacgg caacaggtca actttgttga gcagcatcag cgaggcggcg
     5161 gcaaacatat gcggatattt cagcggtttg tcttcacctt cggtaacgga aagcaccgct
     5221 actttgtgtt tttcaccgag atcgaagctg gccgggcata cgaggttgcc aacgttttcg
     5281 ataaacagaa taccgttatc gtccagtggc agacgcggtg cggcgtcggc aatcatctgt
     5341 gcgtcaagat ggcagccttt accggtgttc acctgaatcg ctggtgtacc ggtagcgcga
     5401 atgcgtgcgg catcgttcac ggtttgctgg tcgccttcaa taaccgcgca cggaacgctg
     5461 tctttcaggc gcattaaggt ttccgtcagc agggtggttt taccggaacc agggctggaa
     5521 accaggttga gcaccagttg cttgcgggca gcaaagcgcg cgcggttgcg ttcagccaga
     5581 cggttgtttt tgtccagcac gtcaatttcg acttccagca tccgacgctg gctcatgccc
     5641 ggtgtgtgag tgcccgcttc gccatgaccg taatgcaggt cgccttcttc agtctggctg
     5701 ggggtaaatt caggcgcttt aatgccggtg attttcatct gcgggcgtgc cgccggggca
     5761 aatggcgcgc tacgaaacgc ggaatgaggg ttatgttcat caccctcgat atacaggttg
     5821 ccttcaccgc aaccgcatgt tgtacacatc gctcactcct ggtctatttc tatccgccga
     5881 atctgtaaac cgtcgtctgc cacaatctgt agcatgtcac catgacactg tggacagcgg
     5941 cggacgcgct gggtcagtag cgtcacatac tgttggcatg tttcacacca gcattcggcc
     6001 tcttgttctt cgaggtgcag tttacaacct tccgccacgc tgccgcggca aaccagatca
     6061 aaacaaaagg caagagagct ggtttcgaca caagaaaatg cgccaatttt gagccagacc
     6121 ccagttacgc gttttgcgcc gtgttttgcg gcctgctgtt cgatcaattc cagtgcccgt
     6181 tggcagaggg ttatttcgtg catatcgcct cccattaact attgccagct acaagcaata
     6241 attgcgccag tgttgattat ccctgcggtg aataatgtcg atgatgtcga aatgacacgt
     6301 cgacacggcg acgaaattca tctttagctt aaaaatctct ttaataacaa taaattaaaa
     6361 gttggcacaa aaaatgctta aagctggcat ctctgttaaa cgagtaacct gacaatgact
     6421 atttgggaaa taagcgagaa agccgattac atcgcacagc ggcatcgtcg cctacaggac
     6481 cagtggcaca tctactgcaa ttcgctggtt caggggatca cgttatcgaa agcgcgcctg
     6541 catcacgcca tgagctgcgc gccggacaaa gaactctgtt tcgtcctttt tgaacatttt
     6601 cgtatttacg tcaccctggc ggatggcttt aacagccaca ccatcgagta ttacgtcgaa
     6661 acaaaagatg gcgaagacaa acagcggatt gcgcaggcgc aactgagcat tgacggcatg
     6721 attgatggca aggtcaacat ccgcgatcgc gaacaggttc tggaacacta tctcgaaaaa
     6781 atcgctggcg tttacgacag cttatacacc gccattgaaa acaatgtgcc ggtgaattta
     6841 agccaactgg taaagggaca aagcccggca gcatgagctg aggctttgcc cgttttgcag
     6901 gcgttacgcc tgtttgggga tgggcgtgtc gatgagtgtc gaaaatgaca tttcatcggc
     6961 atgttttcgt caaaaatgac aatcacctga ggaatgcctg gtgaatcgtt ttgtaattgc
     7021 cgactccacg ctctgtatcg gctgccacac ctgtgaggcc gcctgttcag agacgcatcg
     7081 ccagcacggc ctgcaatcaa tgccgcgcct gagagtgatg ctcaatgaaa aagaatctgc
     7141 gccgcagctc tgtcaccact gtgaagatgc accctgcgcg gtggtctgcc cggttaacgc
     7201 catcacccgc gttgatgggg ccgtgcagtt gaatgaaagt ctgtgcgtaa gctgcaaact
     7261 gtgcggcatc gcctgcccgt ttggcgcaat tgaattttcc ggcagccgtc cgctggatat
     7321 tccggcaaac gccaataccc cgaaagcgcc accggcaccg cctgctccgg cgcgtgtcag
     7381 cacattgctt gactgggtgc caggtattcg cgcgatcgcc gtcaaatgtg acctttgtag
     7441 ctttgatgaa caaggtccgg cctgtgtgcg gatgtgcccg actaaagccc tgcatctggt
     7501 ggataacacc gatatcgccc gcgtcagcaa acgtaagcgt gagctgacct ttaacacgga
     7561 ctttggcgat ctcaccttgt ttcagcaggc tcaaagtgga gaggctaaat gagcgcaatt
     7621 tccctgatca atagcggcgt ggcatggttt gtcgccgccg ctgttctggc atttctcttt
     7681 tcaaaaagcg ctaagtggct ggatagccgg aattggcggc gcggttggta gtctgtatac
     7741 ggcagccgcg ggcttcactg tactgactgg cgcggttggc gtgagcggtg cgctgtcgct
     7801 ggtaagctac gatgtgcaaa tctccccgct taacgcgatt tggctgatta ccctcggcct
     7861 ttgcggtctg tttgtcagcc tctataacat tgactggcat cgccacgcgc aggtgaagtg
     7921 caacggcttg cagatcaata tgttgatggc tgccgccgtc tgcgccgtca tcgccagcaa
     7981 cctcggcatg ttcgtggtaa tggccgaagt catggccttg tgtgcggtgt tcctcaccag
     8041 caacagcaaa gagggcaaac tgtggtttgc gctgaggcgt cttggcactc tgctgctggt
     8101 gattgcttgc tggctgctgt ggcagcgtta tggcacgctg gatctgcgcc tgctggatat
     8161 gcgtatgcaa cagctgccgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
     8221 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
     8281 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
     8341 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
     8401 cggcatgatc accgcgttcg tcggtggtct gtatgcgttg atggaaca
//
LOCUS       DAZSNM010000177         8374 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZSNM010000177 DAZSNM010000000
VERSION     DAZSNM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.177"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..956
                     /gene="espL1"
                     /locus_tag="ACHW42_003291"
                     /pseudo
     CDS             1..956
                     /gene="espL1"
                     /locus_tag="ACHW42_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1128..1235
                     /gene="yniD"
                     /locus_tag="ACHW42_003292"
     CDS             1128..1235
                     /gene="yniD"
                     /locus_tag="ACHW42_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR8944981.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="ACHW42_003293"
     CDS             complement(1288..2046)
                     /locus_tag="ACHW42_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR8944982.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2333..3262
                     /gene="pfkB"
                     /locus_tag="ACHW42_003294"
     CDS             2333..3262
                     /gene="pfkB"
                     /locus_tag="ACHW42_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR8944983.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3363..3653
                     /gene="ghoS"
                     /locus_tag="ACHW42_003295"
     CDS             3363..3653
                     /gene="ghoS"
                     /locus_tag="ACHW42_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR8944984.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3759..4619
                     /locus_tag="ACHW42_003296"
     CDS             3759..4619
                     /locus_tag="ACHW42_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR8944985.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4660..5196)
                     /locus_tag="ACHW42_003297"
     CDS             complement(4660..5196)
                     /locus_tag="ACHW42_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR8944986.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5343..6011
                     /gene="hxpB"
                     /locus_tag="ACHW42_003298"
     CDS             5343..6011
                     /gene="hxpB"
                     /locus_tag="ACHW42_003298"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR8944987.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6174..6764
                     /locus_tag="ACHW42_003299"
     CDS             6174..6764
                     /locus_tag="ACHW42_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR8944988.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6897..8287
                     /gene="tcyP"
                     /locus_tag="ACHW42_003300"
                     /pseudo
     CDS             6897..8287
                     /gene="tcyP"
                     /locus_tag="ACHW42_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8291..>8374)
                     /locus_tag="ACHW42_003301"
     CDS             complement(8291..>8374)
                     /locus_tag="ACHW42_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8944989.1"
                     /translation="YESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         2171 a         2004 c         2009 g         2190 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttctagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattagcca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     2221 tatatatatt tatctgcaaa attttaaata aagctctaat aaatcatatt gttaatttct
     2281 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
     2341 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
     2401 tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa
     2461 cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg
     2521 cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt
     2581 agaagccaaa gactggaccc ggcagaattt acacgtacat gtggaagcaa gcggtgaaca
     2641 gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
     2701 gcaagttctg gaaattgaat ccggggccat cctggtcata agcggaagcc tgccgccagg
     2761 tgtgaagctg gaaaaattaa cccaactgat ttccgctgcg caaaaccaag ggatccgctg
     2821 catcatcgac agttctggcg aagcgttaag tgcagcactg gcgattggta acatcgagtt
     2881 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccaga
     2941 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt
     3001 ttcccttggt ccacaaggag cgctgggtgt cgatagtgaa aactgcattc aggtggtgcc
     3061 accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac
     3121 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
     3181 gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat
     3241 ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa
     3301 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     3361 ctatggccag tggcgatctt gtccattatg tcataaccgt catgttgcat gaggatacat
     3421 tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga
     3481 cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc
     3541 aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag
     3601 atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     3661 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     3721 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     3781 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     3841 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     3901 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     3961 gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc
     4021 tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc
     4081 agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata
     4141 actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg
     4201 ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca
     4261 atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     4321 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     4381 ttttcgaccc tgcctgcgac tggggtgacc gagagtgcga cctagcgatg ttaccgctgc
     4441 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     4501 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaagattat
     4561 ttggcggtca gcatttggtt attgctcagc agtcattgga tagattatta gcagcatgat
     4621 atgggttgag gataatggcc gctccgtgcg gccttttgat taaataaacc caagcagaga
     4681 aaagaacaca taacctgcag caatacaaat aaccggcagg atatatagtg agaaaaactg
     4741 caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc
     4801 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     4861 cgcctggcga ctcatccggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggat
     4921 gaaaatcacc cagaacataa cgttcacacc accattaaaa ttaggggtcg gggagttata
     4981 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagcaaa
     5041 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag
     5101 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     5161 caaaacagca atgcgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt
     5221 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     5281 aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg
     5341 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     5401 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtagata
     5461 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     5521 tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     5581 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     5641 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     5701 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     5761 cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     5821 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     5881 cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     5941 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     6001 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     6061 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     6121 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     6181 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     6241 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     6301 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     6361 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     6421 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     6481 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     6541 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatcctg gttccccaaa
     6601 agggcaacca actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc
     6661 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc
     6721 agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc
     6781 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     6841 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     6901 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6961 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     7021 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     7081 tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc
     7141 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     7201 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     7261 tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     7321 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatcaga
     7381 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     7441 ccaatccgac gtcaattatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     7501 tgaaactgct gaaggatgat gcgccgaaag gggaacgcgt cttaaccgct atcgataccc
     7561 tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     7621 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     7681 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat ggcattctgc
     7741 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     7801 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     7861 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     7921 gtcagaacgg ctgcgccggt ttgtatccgg caatgctgcg gtgatggttg cgcctacggt
     7981 tggcattaac ccgctgggcc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     8041 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     8101 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     8161 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     8221 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     8281 ccgttaattc ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta
     8341 aatgttccat cgcggcgatg catactgact cata
//
LOCUS       DAZSNM010000178         8344 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZSNM010000178 DAZSNM010000000
VERSION     DAZSNM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.178"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(435..641)
                     /locus_tag="ACHW42_003302"
     CDS             complement(435..641)
                     /locus_tag="ACHW42_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR8944990.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="ACHW42_003303"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="ACHW42_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR8944991.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="ACHW42_003304"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="ACHW42_003304"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR8944992.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4489
                     /gene="kdpB"
                     /locus_tag="ACHW42_003305"
                     /pseudo
     CDS             2739..4489
                     /gene="kdpB"
                     /locus_tag="ACHW42_003305"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="ACHW42_003306"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="ACHW42_003306"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR8944993.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="ACHW42_003307"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="ACHW42_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..>8344
                     /locus_tag="ACHW42_003308"
     CDS             8037..>8344
                     /locus_tag="ACHW42_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFR8944994.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     "
BASE COUNT         1793 a         2129 c         2401 g         2021 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggctatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgttaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgagg catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtt
//
LOCUS       DAZSNM010000179         8113 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZSNM010000179 DAZSNM010000000
VERSION     DAZSNM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.179"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(471..1400)
                     /gene="virB"
                     /locus_tag="ACHW42_003309"
     CDS             complement(471..1400)
                     /gene="virB"
                     /locus_tag="ACHW42_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFR8944995.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(1617..1853)
                     /locus_tag="ACHW42_003310"
     CDS             complement(1617..1853)
                     /locus_tag="ACHW42_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFR8944996.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(1857..3758)
                     /gene="ipaA"
                     /locus_tag="ACHW42_003311"
     CDS             complement(1857..3758)
                     /gene="ipaA"
                     /locus_tag="ACHW42_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFR8944997.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(3767..4765)
                     /gene="ipaD"
                     /locus_tag="ACHW42_003312"
     CDS             complement(3767..4765)
                     /gene="ipaD"
                     /locus_tag="ACHW42_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFR8944998.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(4816..5964)
                     /gene="ipaC"
                     /locus_tag="ACHW42_003313"
     CDS             complement(4816..5964)
                     /gene="ipaC"
                     /locus_tag="ACHW42_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFR8944999.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKPTQILYTDISTKQTQSSS
                     ETQKSQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKD
                     LSEMSHTLTPENTLDISSLSSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSPQTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRTYELNTLSAQQKQNIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(5927..7669)
                     /gene="ipaB"
                     /locus_tag="ACHW42_003314"
     CDS             complement(5927..7669)
                     /gene="ipaB"
                     /locus_tag="ACHW42_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFR8945000.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(7675..8112)
                     /gene="ipgC"
                     /locus_tag="ACHW42_003315"
     CDS             complement(7675..8112)
                     /gene="ipgC"
                     /locus_tag="ACHW42_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFR8945001.1"
                     /translation="MSTAVIDAINSGATLKDINAIPDDMMDDIYSYAYDFYNKGRIEE
                     AEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKNDYTPVFHTGQ
                     CQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
BASE COUNT         2173 a         1325 c         1587 g         3028 t
ORIGIN      
        1 ggaagattag gagaataaaa cagtaattat atttgagaaa tctgctaata tattcttaaa
       61 gcttttttga tactgtgaac gaagtctgtt aatggtggat tttttgttta tcaccatgta
      121 aaaaaaattg agtagtaaaa gcgttgactg ctaatcctcg ctgtttttat tgaaacgatt
      181 aaaaatacgc tgaatatttt cggccagtca ctcatattca cataaaattt tgaccatcat
      241 gccgcatccc ttagttgcac aattcatttt caatgatatg aatattacta atttcaccag
      301 ataattttct cgtggtcagc agggtatgag ttttcaccgt aattttaagg aaacacagtg
      361 cgtggggatt gaataacaga tagccgacgg aattacccca ccggctgaaa gtcaagttca
      421 acgaagattt ttattatctg aattgggcag tttacatcag tgttcgatgt ttatgaagac
      481 gatagatggc gagaaattat atcccgaata gcttcatcta tttctctctg aactgttttg
      541 tttattttag ataatgtaaa tattagagtt ctgtttgttt tattctcttt tctttttatg
      601 taagtccgtt tgtctttaga aataaataac gagtcaactt ttagcgaagg gttttgcttt
      661 ctgtttttgc ttttctttat gatgtttaaa atttcttttt tatatatgtc cgggggcaaa
      721 tttgtatcaa ggtcttttat ttcttccttt aatattggta gtgtagaact aagagattca
      781 ttagcctttt caagtccttt ataataattg aataatatct tgtagtcatt aaagttaagt
      841 tctgacgcga ttggaaatag agatattatt tcctgtggaa cgcttgctgc ctgaaaggca
      901 cgagtgactt tcgcgcgaga cagattctct tttttggcta tgtctttata ggacatccct
      961 gatactttca gaaaattaag accaatacca agttctcgga tgctatgctc ttttgctgtt
     1021 tgtatatcgt ttgctagttt tctggcatca agagtagata tatattcttt tgaatataga
     1081 acttcaagat ctgctcctgc atatattgca gatgctcttc tacgagtgcc atccagaatt
     1141 tcaattctac catcaatctc ccttcctatt acagggaaga attgttgtag ctttatagtt
     1201 tttatgatat cggctaatga ttcttctgtt agggattctt gatcgcgtac attcgttttt
     1261 tggttgacga aggttaaatc ttggatcctt ttatgaggaa tcttggcttt gataaaggaa
     1321 actttattac cagaatggag tgtaaactct ttcttttggc cttcctttat acttaacaag
     1381 tcgttgcaca aatccaccat atcacaccct gtttattcat attgatttat attgacaaaa
     1441 gttaaatgca gtgtgagcaa gattgtatgt aactgttgat gatagtcaac ggtagtctta
     1501 aaaattacta ttttaatagc tggattgatg caatttatct ttattggata aaagaataat
     1561 attcttgtgg agctctgatg tgagagctcc atagatgaaa aaatgcttat caattactat
     1621 attgattttg tactattttt gactacagaa ataagatctc caatagtaat aatgtgttct
     1681 aatgtagatt catctatacg tagattgaac tcaaaactaa tcatatccag catttcaatc
     1741 agagaaagag aatcggcaga aagatcttca ataaatttag tttcttcaga tagtttgctg
     1801 tacgctatac catagcaaaa ggccactatt gataatattt tttcttttat catattttaa
     1861 tccttattga tattctttaa tacttttgat agggaagtgg ttacatcttt tgctgcttta
     1921 tatatattat tgttttcagc tgtaatatca ttgttatttt ttaaatcaga tatatcatct
     1981 gtaagtaatt ctgcagaggt atcgtcaatt tttgacaata ccttgctgag ggcgctagat
     2041 acttcttttg ccttttcata gatcgcatga tttttatcaa ttgtatctgc gttggtttct
     2101 gttacgtagt ttcttatatt tgcatctgta acttttaatt tgttgtttat tctatgttct
     2161 gttgtggatt ttgtaatgtc ctttgatttt tcctgtaact ctcgttcttg tgttcctgat
     2221 ttagttccaa tgagagatat aagattactt agggagtttg ttactttttt tgaagcttca
     2281 aatatcgttt cccgtgttaa atatgtgtct ccatttcttg ataaaaaatc agtctgaagt
     2341 acttttgaat cagtgtttga gttgaattta ttgatttgcg aagctactct atgagaaaga
     2401 gaagcgctac ttttagttgt atctgatgag ttatcattac tgcttgtcac tccttccggg
     2461 tggagagggg gggtggttga agagttctgt atgttcagag gcctgtgtcc ccgagaaaga
     2521 gagttatttg cagatctttc ttgactactc gtagttaaca ggtctgaacc tattccatcc
     2581 gaattatctg gagtatggtt gaaagccttt tctaaagctg aattatttgc aaatgtctgg
     2641 acagtatctg atatagagtg tggtagaacg gaagttactt tttgaactag tgctctttgt
     2701 ggatttagta atgagtttcc gttacgggat aatagatcgt ttgtctgaga agtaggagac
     2761 tgtgcatcat tttctgggtt ctcgtgatag ctattgtcat atactctgtt gtcaaaaact
     2821 ctattatcgt aagttctatt atcattattt atatgatagt gaattacagg ttgggatgtt
     2881 ttctcattgg ctccgggggt tggtccgcca tctggcatat cttcgggagg tgtagggagg
     2941 ccatcttgga taggatgcga atcttttata tcttctaaaa tatcattaag gacttttctc
     3001 aacagctcag gaaacacggc tactgtattt aagtctcttg acagtttacc tataccaaat
     3061 cctttactat caatagtatt tttacgttga ttgcgcaaac tatcaaaggc aagttttgcc
     3121 tgtgcgtcaa ctaaatcatc tagtttgaga gatgaaacag tttcattact taactttaat
     3181 tcagcaagaa tattattctt gataaatgat gatacttcag tattgaggag tttttgtccc
     3241 aatgataaag cttcaccaga tgtgtgttgt tgtgctaata gtgcaagtct gtgctcaatg
     3301 ttttgtcgta attgatggag tttcttattc tttgccgacc atggtccaaa ggcagaatct
     3361 tgggataatt tatcttttat aattagttcc gaatactgtg cgacaatttt ttgaagtccc
     3421 ttttcgtttt caaaattaag accattttga tgtactaagg agtacaaaac atttgaaaat
     3481 tcgagaatct cgcttttggc atacgcattc attgagttgg aataaatgct tcttaacaca
     3541 tcttctttcc ctgaggaaga aaataaaaaa tcaagacatt tctgattaaa agacgttgca
     3601 aagttttctt tttcagacac tgatgctggt gtttttagag aagtttgcga actatggata
     3661 tcggatattt tgctttttaa ctcgctgtat tctgttgatg atggtgaagt tgccttatat
     3721 aagaatgttg gcgcttgagt attatttaca ttatgcatgg cgcacctcag aaatggagaa
     3781 aaagtttatc tgtatctgta catgagctta ttgtactact caaaaccttt actaaattat
     3841 caaaaatact attggcatta ctgtattttt gaactaaagt ttgaagatta tttttcattg
     3901 tttcatcttc ggcagagaat ccggcattcc atgcctgata ttttgcatta tctagcacaa
     3961 cctcgccatt tccacctaga ttatctaagc tttttaacat attgtctatt ggggtcatgt
     4021 ttatactgac aacatatccc ccgttttttt gagatacctt gccgattgtt ccacctaatt
     4081 ctgtaagcca tttatttgct tgttcctgac taacagtatt atttgctgga tatagcggtt
     4141 tatctttata tttttccttg agttcttcca atgccttttt aagcgagttg acttgtaatt
     4201 tcacggagtt tccgtcgtta cctccgggag agatccagcc ggcaagactg gaaagaacag
     4261 cgctaaaatc ttgatacatt tgagtatatg aactaacggc atgttcatat actttcagat
     4321 actgttcatt aatatcattg atggagtttg caattttagc ccacagttct ctatgagata
     4381 tcatttgatc tccatcaagc tcggcttctt tcggagctga atgtaataat tctcttgcgt
     4441 ctttattaat tggatattcg tgcctggaaa gaatatctaa tagttgagcg gatttattta
     4501 catccatgct aatctgagat gaatgtaatg ctatttcttc tagcgatgtt ttagtcaacg
     4561 ttttttgtga aagctctttc tttaatgcct gatttgttgt tctgatatta tgaagggtgt
     4621 cgttgagcat agtaagggaa cttacaggat gagaactggt cgttgttttt atatcagaat
     4681 taactgtttc ggttgatgaa ccgttggtat tgtttggact gaatgatgag gtggaaatac
     4741 tattagtcag agttgttata ttcatggtta tttccttaca ggaagagcca tatattatta
     4801 tggctcttgg atatattaag ctcgaatgtt accagcaatc tgactggctg tcgaattctt
     4861 tgattggttg atgctgtcaa ttatattcaa taatttttgt attaattgat ttgtcgcttg
     4921 ggatgcttct ttagatactt gggatgcttc ctctgcttgt ttactgctgg cctgactgat
     4981 tagttgttct tcttcttcaa gagcagatgc ataacgccct cctgatgtgg atatattacc
     5041 agcaacggct gatgtttcca ttgttgcacg gccaatgttt tgtttttgct gcgcagaaag
     5101 ggtattgagc tcgtaagttc ttctctgggt gtcaatttta tcctgcaaag aaatatcggg
     5161 agaactaaga gaagttttat gttcagttga cagggatata ttatctggcg ccagtttatt
     5221 tttacctaaa aattttgtgc tagagttagt ttgtggtgag gtgatatttg tatctatttg
     5281 tttatttaac cctaatttag aacctgcaag ctctttttca agagattgag cagtggcaag
     5341 gttctttctt aaggctcctt tttggtcgct aatccctgaa tgcgtttttt tggcaccgat
     5401 acccgttata cctacttgtg tgactgctcc agtaatgctt gatgataggg ctgccaggcc
     5461 ttgccgaaca atgttctctg cagctgattt tgtagcatcg aacgcaatca atgacaattg
     5521 agagcccaat ttagtttctg cagtgcggag agcagaaagt agaacggcta cactaataat
     5581 taaagaaaca gcattagaag aaagactgga aatatccaga gtgttctctg gagtaagggt
     5641 gtgactcatc tctgaaagat ctttcatctt tttgtcagta aggcgagcaa tgatttctga
     5701 atcatgctga acctgctctg ataactttaa aagttgatca tcatttaatg tggttgttaa
     5761 tacgggattt ttaccgacat taagtggaat atgcgctgca atctgctgat aattttgtga
     5821 tttttgtgtt tcggaagaac tttgagtttg ttttgtggat atatctgtat ataaaatctg
     5881 ggttggtttt gtgttttgaa tttccataac attctcctta tttgtatcaa gcagtagttt
     5941 gttgcaaaat tgcttttgca acatcagttc tattagcctg agagttggac attgaggcta
     6001 atagatctgc aattacttcc tgcaattggc cgaatttttc tattgcttca ctgatatatt
     6061 ttgacagttg ttcaacttga tatttcgata atgtcaggtc tgctagattt gtcgacgcgc
     6121 tgttctggaa aacagcagaa gcgacacttc ctcccgcttg tgtggcagag ttaacacttt
     6181 ctcctaataa aactgcttgg tttaaatggg tggaaataat ttgtttggat attacttcat
     6241 cacccgctgc accaagaaat ttatttaatc tggcaacagc attagtgatt aaatcgtcca
     6301 gttgagaaga aaaattcttg agaaactttg gtataaggtc tgtgagggtt ttacctatta
     6361 ttttgccaat attttctgca agttttgctg ctgcctgttt accaacagtg gctacgagaa
     6421 cgactgctgc aactaggaca agagcgcctg cgattgcccc cagaatagag ccaatcattt
     6481 tggctttttt cgagtcgacg cccaagcctt cgagcatttt tgtaaatgca tctgaaagga
     6541 gtttgattaa gggttcaatg actgctttca tgatcggatt cagggcttgt tccatgaagg
     6601 aattgccggt cgctgcttgt actatagcat ccgtaaccat aagagctaaa ccaacagctg
     6661 ccagtgctag agaggctcct ccagaaaaag ctgctgcaac aacactaacg atagttaaaa
     6721 gtgccccaag tattttccca acacaaccca ttactctgtt gagttcttct gctttacgta
     6781 cttcagcagc atactcatca gattttctct ccatttcagt ttttcttgat tcttggagag
     6841 actggaatag agccagatca ttttttaaag attcttcatt attttttcca actagttgaa
     6901 taaaggttgc catcaattga gtaacactgg caagtccggt taatgatttc tgctgggttg
     6961 atagctgttc agcagatgct gtgtttgaaa atgcagaaaa agagtctatt tctttttcga
     7021 gttgcattga tttatctgta agtttgctat gaattgacag ggttttctgc tcaatcaatg
     7081 tcctgtcttt aactgctgcg tcttttttga tagtgagttg tatttcttcc cggcttaatt
     7141 ttttcttttc tggtgactct gggtcgagtt cggataatct tgtttgaatt tggttaattt
     7201 tattttctag gtcttttatt ttagaatctg cgttttttag tttattaatt tgtttttcat
     7261 agtctctggt tagtccttca gtttcagata gaagagtgtt aattttatcg gagaattcta
     7321 ggtttttttg ctgtcttgcc tgttgctggg acttccaagc agtaatttta tttgttaatg
     7381 cagttaaaga tttttcaccg agtatttgaa taaggtttcc aattaaaagc gttagttggg
     7441 aacttgcatt taatgacttt ggtgctttaa gttcagggat taatatattt gaagttgagt
     7501 gtgcattagt tgtattaata ttttggttag cagtaagatc agcaattgta agagaaaata
     7561 atttgttagc tgcatcattt gcagcttgga tagtattgtc tccaagctca gtggaagcca
     7621 atattttggc aagaggaaaa ccagtggttg tggtgcttac attatgcata ataattactc
     7681 cttgatatcc tgaattgcgt ccaagtatga ttgtgctttt atttttaatt tttcatcatt
     7741 gctgtgttga attacgagtt cgaagcactc tttagctttt aagggggctt tcaaccgaag
     7801 ctggcattgt ccagtatgga atactggtgt atagtcattt tttcctaatg caaaagcgac
     7861 agcataaagg tctgctgctt gttggaactg ttcttttatc tgataaatag ctgcgagtcc
     7921 cataatgtag tctacattgt aaaagtcgta tatacataaa aacctgaaga aaacttcagc
     7981 ttcctctatt cttcctttgt tgtaaaagtc ataagcatat gaataaatgt catccatcat
     8041 atcatcagga attgcattaa tatctttcag tgtagcgcca gagttaattg catcaattac
     8101 tgcagtagag atg
//
LOCUS       DAZSNM010000180         8102 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZSNM010000180 DAZSNM010000000
VERSION     DAZSNM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.180"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..409)
                     /gene="aspC"
                     /locus_tag="ACHW42_003316"
     CDS             complement(<1..409)
                     /gene="aspC"
                     /locus_tag="ACHW42_003316"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR8945002.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSV"
     gene            complement(594..1682)
                     /gene="ompF"
                     /locus_tag="ACHW42_003317"
     CDS             complement(594..1682)
                     /gene="ompF"
                     /locus_tag="ACHW42_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR8945003.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(2283..3683)
                     /gene="asnS"
                     /locus_tag="ACHW42_003318"
     CDS             complement(2283..3683)
                     /gene="asnS"
                     /locus_tag="ACHW42_003318"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR8945004.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(3852..5054)
                     /gene="pncB"
                     /locus_tag="ACHW42_003319"
     CDS             complement(3852..5054)
                     /gene="pncB"
                     /locus_tag="ACHW42_003319"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR8945005.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            5320..7935
                     /gene="pepN"
                     /locus_tag="ACHW42_003320"
     CDS             5320..7935
                     /gene="pepN"
                     /locus_tag="ACHW42_003320"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR8945006.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
BASE COUNT         2083 a         2012 c         2032 g         1975 t
ORIGIN      
        1 agacgctctt atggttcggc cagcttgggt tgctcaccca cacacgctta acgctggtat
       61 tttttgccag gaagtcggca gccacgcgta gtgcgccagt gccacccgga gtctgtgccg
      121 tgcgagcacg tttgtcattg atcagggcgc tacctttacc aaacagcagt tcctgagtgc
      181 agcgaccaaa ttcaaggatg ccgtcaatgc cgaggtaatt tttggtggtt tcattttcga
      241 gcagatactg ttcagccttt ttcacgctgg tcagtaccgg ggttttgccc gtctcatctt
      301 tatagacacc aatcccgagg ttaattttgc cgggacgttc atcggcacga aacagatcgg
      361 ccaggcccag aatcgggtcg gcaggagcgg cggtaatgtt ctcaaacatg acgaggttcc
      421 attatggtta cagaagggaa gtccgctatc agggtaacgg gagatttaca aaattccaac
      481 tattactgat gaaaacgcag gctgtttttg caagacgtga gattgctctg gaaggcataa
      541 aaaaaacagg accaacgtcc tgttttttcg gcatttaaca aagaggtgtg ctattagaac
      601 tggtaaacga tacccacagc aacggtgtcg tctgaaccta cgcccagttt gttgtcagaa
      661 tcgatctggt tgatgatgta gtcaacatag gtggacatgt ttttgttgaa gtagtaggtt
      721 gcgcccactt caaagtagtt caccagatca acatcaccga taccttctac gtctttcgct
      781 ttagatttgg tgtaagcgat ggacggacgc agaccgaaat cgaactggta ttgcgcaact
      841 aacagaacgt cttgcgtttt gttggcgaag ccgctggtgt ttgtaaattt attagtgatc
      901 ggcgtagcgt tacgggtttc accgtagttc gctgccaggt agatgttgtt cgcgtcgtac
      961 ttcagaccag tagcccactg ttcagctttt ttaccgttgc caagaggttg agcttcttgc
     1021 aggttggtac ggtcagctgc accataagca ccaacgatac caaagccttc gtattcgtag
     1081 ctgatagaac cgccaacacc gtcgccgtta gaacggcgtg cagtgtcacg ctcgttttta
     1141 cccaggtact gaacagcgaa gttcaggcca tcaaccagac caaagaagtt ggagttacga
     1201 taggtagcaa cgccgccaac acgaccaacg aagaagtcat cgctgtatgc agtatcacca
     1261 ccaaattctg gcagcatatc ggtgtaaccc agtgcatcat aaaccacacc gtagttacgg
     1321 ccgtaatcga aagaaccaac gtcagcgtat ttaagacccg cgaatgccag acgcgttttg
     1381 ttaccagttt gagcgtcagc gccttcagag ttgttaccct ggaagttata ttcccactga
     1441 ccataaccgg tcagatcgga attgatttga gtttcccctt taaaaccaag acgggcatag
     1501 gtcatgtcgc cattgccacc gtaactgttt tcaccgttac ccttggaaaa ataatgcaga
     1561 ccaacagttt taccgtacag atctactttg ttgccatctt tgttatagat ttctgcagcg
     1621 tttgcagtac ctgctactaa cagagcaggg acgatcactg ccagaatatt gcgcttcatc
     1681 attatttatt accctcatgg tttttttatg acacctgcca ctgccgtcaa taagttctgt
     1741 caataaaaat ttacggaact attgatgaga gtttggtgtc tttatgtgtc tgcaggcatc
     1801 tttccattca aactaacgtt tcgctaccgt gaaagtgcta caaagataaa gatttagttt
     1861 caaaaagaaa aaatatgtaa ccaaaagtaa aaattaagga gctttgtgaa caccgtcata
     1921 tttccatagg aacgtgatga tatttacagc aatttttatc tatttatatg atttacttat
     1981 atttaaatta actaaacgga aattttgttt ctgatggaaa ctttatcgac ctggcacaaa
     2041 atcctctttc agacatccag aatgccaaaa aatagtatga aaattgtgct atcagctaag
     2101 aaaaaactaa tccgcaataa gagtacgggt tattttttgt gcgtaaatgt tgcaatgtaa
     2161 tattccgtaa caggatgatc gttatggcgg gggtgcaaat agattgcttg acttaacaaa
     2221 cagataactt gacagaaagg ataaaaaaga gccagcaggc gctggctctt tgaggaagaa
     2281 aatcagaagc tggcgttgcg cggagtacgt gggaacggaa tcacatcacg cacgttttgc
     2341 acgccagtta cgtaagcaat caggcgttca aaaccaagac cgaaacctga atgcggaaca
     2401 gtaccgtagc gacgcagatc gcgataccac cagtaatctt ctttattcag gcccatttcc
     2461 agcatacgct cgtccagaac gtccagacgt tcttcacgct gggagccacc aatgatctca
     2521 ccgatgcccg gagccagaac gtccatagcc gcaacggttt taccgtcttc gttgaggcgc
     2581 atatagaacg ctttaatatc tttcggatag tttttaacca ctaccggtgc tttaaagtgt
     2641 tcttccgcca gataacgctc atgctcagaa gagagatcga cgccccagta aaccgggttt
     2701 tcaaacttcc tgccgcagtt ttcgagaatg gtcactgcgt cggtgtaatc cacctgcgcg
     2761 aaatcggctt caatgaagcg ttccagacgt gaaacggcat ctttatctac gcgttcagcg
     2821 aagaatttca tgtcgtctgc gcgttcttcg agaaccgctt tgaagacata tttcagcatg
     2881 gcttcagcca gacccgcgat atcgttcagg ttagcaaacg ccacttccgg ctccagcatc
     2941 cagaattccg ccaggtgacg gctggtgttg gagttttcag cacggaaagt cgggccgaag
     3001 gtataaattt tggataatgc gcaagcgtag gtttcgccgt tcaactggcc agatacggtc
     3061 aggaaagact ctttaccaaa gaagtctttg tcgaaatcca ctttgccctg atcgttacgc
     3121 ggcaggtttt ccagatccag cgtagaaacg cggaacattt cgcctgcacc ttcggtatca
     3181 gatgcggtaa tcagtggcgt tgaaacccag aagaatccct gctcgttaaa gaagcgatgc
     3241 agcgcctgcg ccagcgtatg gcgaacgcgc gcgacggcac caatcaggtt tgtgcgcgga
     3301 cgcaggtgag cgacttcacg cagatactca atgctgtggc gttttgccgc catcgggtaa
     3361 gtgtctggat cttcaaccca accagcaact tcaaccttgc tggcctgaat ttcaaattgt
     3421 tgcccctggc ccggcgatgc cacgacttta cccgtcacaa tgaccgagca gccggtggtc
     3481 agacgcagga cgtcttcatt gtaattgggc agagaattat tgatgacagc ctgtacagga
     3541 tcaaagcagg aaccgtcata aacggcgagg aaggagatgc cagcttttga atctcggcgg
     3601 gtacgtaccc atccgcgtac ggtgacttcg ctgtcaacgg ctacgcggcc ctggagtacg
     3661 tcggctacag gcacaacgct cataatattc tctctgttaa tagtcggaaa aaataaacac
     3721 ttgtccaccc gtaaatgggg gggataccta tgttacctgg catctgcaat cagacaagca
     3781 gaattcgcaa atgcagcgaa agatttcgga attaaaggta aaaaagggag ccgattagct
     3841 ccctgatgat attaactggc ttttttaata tgcggaaggt cgaacgcttt gcgcagcgcc
     3901 cgaacaaacg ctttatcatg gcagatagtt ttgccagggc tgtcagaaag tttcgccacc
     3961 ggtttaccgt tacactctac caacttaatc acaatattca ggggttttac ctgggggata
     4021 tcgcaggtca ggcgagtccc aataccaaaa cttaattgca cgcgggaaga gaagtggcgg
     4081 tatagctcaa ccgctttgcg taaatccaga ttgtcagaga aaaccagcgt tttactctgt
     4141 ggatcaattc ccagcttttc ataatgtgca atggcttttt caccccattc aaccgggtcg
     4201 ccagagtcat gacgcaggcc ctgataccga ctagcgaact cgacaccgaa atcacgcagg
     4261 aaagcatcca tagtgatgca gtcggttaat gcaatgccaa gttggtcggg atactcttcc
     4321 agccaggcag caagtgcagc tcgctggctg ttggctagat ccgggctgat ttgctgatgt
     4381 gcctggaacc attcgtgtgc ctgtgttccc atcggcgtga gggaaagccg acgcgccaga
     4441 tcgtagttgc tggtgcccac gaaccaggat tcctgttgca gacgcttaac gatggtttct
     4501 tgtacttcgc gagaaaaacg gcgacgggtg ccaaaatcca tcagatggaa gcgcgacata
     4561 tcaagaccgg cggttaacgc cgagaagtcg actaatttgc tttccagcgt gtcgagggct
     4621 tgcgcaacgt cggcctgcgg tgagcgatag cgatgtacca tttcactgat aaccgccagc
     4681 aaaggaactt cccagaggat gacttcacgc cacggaccgc ttaagcgaat atccagcttg
     4741 ccattatcgt tggacacggt gacttgttcc gggttaaagc ggaactcgcg taaccagtta
     4801 agatagtcgg ccttaaagaa aggcagggca gagagccact gatattcatc atcctgcagg
     4861 cgcaggtgct gcatcgcctg aatctgttca cgaatagcat cggcataaat acccagcaga
     4921 tcgtcacctc ggcaacgaaa ctccgccgcg acatgcacat cgtaatagtg atgaaacacg
     4981 gcttgctgca tatgcaactt ataagcatct gtatccagca acgagtgcag aacaggagaa
     5041 gcgaattgtg tcataggtgc gcagtagcgt cctcgtacag gagcgtttag tacaataaac
     5101 atcttcagga actgctggag tataccttgt ttcgcaattt attgaacccc gatcacacca
     5161 tatgccacct ttctggtcga tggcattacg cggtgtatgt tataaaaatg tagcaataaa
     5221 ggcgtttgta cctgaaaaga tgaagattct gcatagcgcg atttacgcaa caggaataga
     5281 ctgaacacca gactctataa aagatgctaa aggttattta tgactcaaca gccacaagcc
     5341 aaataccgtc acgattatcg tgcgccggat taccagatta ctgatattga cttgaccttt
     5401 gacctcgacg cgcaaaagac ggtcgttacc gcggtcagcc aggctgtccg tcatggtgca
     5461 tcagatgctc cgcttcgtct caacggcgaa gacctcaaac tggtttctgt tcatattaat
     5521 gatgagccgt ggaccgcctg gaaagaagaa gaagagggcg cactggtcat cagtaatttg
     5581 ccggagcgtt ttacgcttaa gatcattaat gaaataagcc tggcggcgaa tacggcgctg
     5641 gaagggcttt atcagtcagg cgatgcgctt tgcacccagt gtgaagccga aggtttccgc
     5701 catattacgt attatctcga ccgcccggac gtgctggcgc gttttaccac caaaattatt
     5761 gccgataaaa tcaaatatcc cttcctgctt tccaatggta accgcgttgc gcaaggcgaa
     5821 ctcgaaaacg gacgccattg ggtacagtgg caggacccgt tcccgaaacc gtgctacctg
     5881 tttgcgctgg tggcaggcga ctttgatgta ctgcgcgata cctttaccac gcgttctggt
     5941 cgcgaagtag cactggagct gtacgtcgat cgcggcaacc ttgatcgcgc gccgtgggcg
     6001 atgacctcgc tgaaaaactc catgaaatgg gatgaagaac gctttggcct ggagtatgac
     6061 ctcgacatct atatgatcgt cgcggtggat ttcttcaata tgggcgcaat ggagaataag
     6121 ggtctgaata tctttaactc caaatatgtg ctggcccgca ccgacaccgc caccgacaaa
     6181 gattacctcg atattgaacg cgttatcggc catgaatatt tccataactg gaccggtaac
     6241 cgagtgacct gccgcgactg gttccagctc agcctgaaag aaggtttaac cgtcttccgc
     6301 gatcaggaat tcagctctga ccttggttcc cgcgcagtta accgcatcaa taatgtgcgc
     6361 accatgcgcg gattgcagtt tgcggaagac gccagcccga tggcgcaccc aatccgcccg
     6421 gacatggtca ttgagatgaa caacttctac accctgaccg tttacgagaa gggcgcggaa
     6481 gtgatccgta tgatccacac cctgctgggc gaagaaaact tccagaaagg gatgcaactc
     6541 tatttcgagc gtcatgatgg tagcgcagcg acctgtgacg actttgtgca ggcgatggaa
     6601 gatgcgtcga atgttgatct ctcccatttc cgccgttggt acagccagtc cggtacgccg
     6661 gttgtgaccg tcaaagacga ctacaatccg gaaactgagc agtacaccct gaccatcagc
     6721 cagcgcacgc cagccacgcc ggatcaggca gaaaaacagc cgctgcatat tccgtttgcc
     6781 atcgaattgt atgacaacga aggtaaagtg atcccgttgc agaaaggcgg tcatccggtg
     6841 aattccgtgc tgaacgtcac ccaggcggaa cagacctttg tttttgataa tgtctacttc
     6901 cagccggtgc ctgcgctgct gtgcgaattc tctgcgccag tgaaactgga atataagtgg
     6961 agcgatcagc aactgacctt cctgatgcgt catgcgcgta atggtttctc ccgttgggat
     7021 gcggcgcaaa gtttgctggc aacctacatc aagctgaacg tcgcccgtca tcagcaaggg
     7081 cagccgctgt ctctgccggt acatgtggct gacgctttcc gcgcggtgct gctcgatgag
     7141 aagattgatc cggcgctggc ggcagaaatc ctgacgctgc cttctgtcaa tgaaatggct
     7201 gaactgttcg atatcataga cccgattgct attgccgaag tacgcgaagc actcactcgt
     7261 actctggcga ctgaactggc ggatgagctg ctggctattt acaacgcgaa ttaccagagc
     7321 gagtaccgtg ttgagcatga agatattgca aaacgcactc tgcgtaatgc ctgcctgcgc
     7381 ttccttgctt ttggtgaaac gcatctggct gacgtgctgg tgagcaagca ataccatgaa
     7441 gcaaacaata tgaccgatgc gctggcagcg ctttctgcgg cggttgctgc acagctgcct
     7501 tgccgtgacg cgctgatgca ggagtacgac gataagtggc atcaggacgg tctggtgatg
     7561 gataaatggt ttatcctgca agccaccagc ccggcggcga atgtgctgga gacggtgcgt
     7621 ggtctgttgc agcatcgctc atttaccatg agcaacccga accgtattcg ttcgttgatt
     7681 ggtgcgtttg cgggcagcaa cccggcagcg ttccatgccg aagatggcag cggttaccag
     7741 ttcctggtgg aaatgcttac cgacctcaac agccgtaacc cgcaggtagc ttcacgtctg
     7801 attgaaccgc tgattcgcct gaaacgttat gatgccaaac gtcaggagaa aatgcgcgcg
     7861 gcgctggaac agttgaaagg gctggaaaat ctctctggcg atctttacga gaagataact
     7921 aaagcactgg cttgatactt ttgagccaaa atgcctgatg cgacgcttga cgcgtcttat
     7981 caggcctaca actccgttga aattgctggt tttgtaggct ggataaggcg ttcacgccgc
     8041 atccggcagc cgtgcccaac tgcctgatgc gatgcttgac gtgtcttatc aggcctacaa
     8101 gt
//
LOCUS       DAZSNM010000181         8085 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZSNM010000181 DAZSNM010000000
VERSION     DAZSNM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.181"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..724
                     /gene="ygaZ"
                     /locus_tag="ACHW42_003321"
     CDS             <1..724
                     /gene="ygaZ"
                     /locus_tag="ACHW42_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR8945007.1"
                     /translation="PQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLGFSPLE
                     SVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRIIQRLQK
                     SKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGAFSGSG
                     LLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIPVAILA
                     GIVCGCLTALIQAFWQGAPDEL"
     gene            714..1049
                     /gene="ygaH"
                     /locus_tag="ACHW42_003322"
     CDS             714..1049
                     /gene="ygaH"
                     /locus_tag="ACHW42_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR8945008.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            1140..1670
                     /gene="emrR"
                     /locus_tag="ACHW42_003323"
     CDS             1140..1670
                     /gene="emrR"
                     /locus_tag="ACHW42_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR8945009.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            1797..2969
                     /gene="emrA"
                     /locus_tag="ACHW42_003324"
                     /pseudo
     CDS             1797..2969
                     /gene="emrA"
                     /locus_tag="ACHW42_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            2986..4524
                     /gene="emrB"
                     /locus_tag="ACHW42_003325"
     CDS             2986..4524
                     /gene="emrB"
                     /locus_tag="ACHW42_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR8945010.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(4588..5103)
                     /gene="luxS"
                     /locus_tag="ACHW42_003326"
     CDS             complement(4588..5103)
                     /gene="luxS"
                     /locus_tag="ACHW42_003326"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR8945011.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(5253..6809)
                     /gene="gshA"
                     /locus_tag="ACHW42_003327"
     CDS             complement(5253..6809)
                     /gene="gshA"
                     /locus_tag="ACHW42_003327"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR8945012.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(6882..7310)
                     /locus_tag="ACHW42_003328"
     CDS             complement(6882..7310)
                     /locus_tag="ACHW42_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR8945013.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(7307..7873)
                     /gene="yqaB"
                     /locus_tag="ACHW42_003329"
     CDS             complement(7307..7873)
                     /gene="yqaB"
                     /locus_tag="ACHW42_003329"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR8945014.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         1898 a         2144 c         2036 g         2007 t
ORIGIN      
        1 tccacagcct gctcctggtt cggcgacctt catggaagga tgcaaagaca gtttaccgat
       61 tgttattagt tatattccgg tggcctttgc gttcggtctg aatgcgaccc gtctgggatt
      121 ctctcctctc gaaagcgttt ttttctcctg catcatttat gcaggcgcga gccagttcgt
      181 cattaccgcg atgctggcag ccgggagtag tttgtgggtt gctgcactga gcgtcatggc
      241 aatggatgtt cgccatgttt tgtatggccc gtcactgcgt agccgtatta ttcagcgtct
      301 gcaaaaatcg aaaaccgccc tatgggcgtt tggcctgacg gatgaggttt ttgccgccgc
      361 caccgcaaaa ctggtacgca ataatcgccg ctggagcgag aactggatga tcggcattgc
      421 cttcagttca tggtcatcgt gggtatttgg tacggtaata ggggcattct ccggcagcgg
      481 cttgctgcaa ggttatcccg ccgttgaagc tgcattaggt tttatgcttc cggcactctt
      541 tatgagtttc ctgctcgcct ctttccagcg caaacaatct ctttgcgtta ccgcagcttt
      601 agttggtgcc cttgcaggcg taacgctatt ttctattccc gtcgccattc tggcaggcat
      661 tgtctgtggc tgcctcactg cgttaatcca ggcattctgg caaggagcgc ccgatgagct
      721 atgaggttct gctgcttggg ttactggttg gcgcggcgaa ttattgcttc cgctatttgc
      781 cgctgcgcct gcgtgtgggt aatgcccgcc caaccaaacg tggcgcggta ggtattttgc
      841 tcgacaccat tggcatcgcc tcgatatgcg ctctgctggt tgtctctacc gcaccagaag
      901 tgatgcacga tacacgccgt ttcgtgccca cgctggtcgg cttcgcggta ctgggtgcca
      961 gtttctataa aacacgcagc attatcatcc caacactgct tagtgcgctg gcctatgggc
     1021 tcgcctggaa agtgatggcg attatataac ccacaagaat catttttcta aaacaataca
     1081 tttactttat ttgtcactgt cgttactata tcggctgaaa ttaatgaggt catacccaaa
     1141 tggatagttc gtttacgccc attgaacaaa tgctaaaatt tcgcgccagc cgccacgaag
     1201 attttcctta tcaggagatc cttctgactc gtctttgcat gcacatgcaa agcaagctgc
     1261 tggagaaccg caataaaatg ctgaaggctc aggggattaa cgagacgttg tttatggcgt
     1321 tgattacgct ggagtctcag gaaaaccaca gtattcagcc ttctgaatta agttgtgctc
     1381 ttggatcatc ccgtaccaac gcgacgcgta ttgccgatga actggaaaaa cgcggttgga
     1441 tcgaacgtcg taaaagcgat aacgatcgcc gctgcctgca tctgcaatta acggaaaaag
     1501 gtcacgagtt tttgcgcgag gttttaccac cgcagcataa ctgcctgcat caactctggt
     1561 ccgcgctcag cacaacagaa aaagatcagc tcgagcaaat cacccgcaaa ttgctctccc
     1621 gtctcgacca gatggaacaa gacggtgtgg ttctcgaagc gatgagctaa cgcgtcatct
     1681 cgctcaaaaa tccagattta taaaagaaaa aatgaccggc cagcatcgca acatgctggc
     1741 ctttttggca agcaggtcgg ctcagccgat gagttaagaa gatcgtggag aacaatatga
     1801 gcgcaaatgc ggagactcaa accccgcagc aaccggtaaa gaagagcggc aaacgtaagc
     1861 gtctgctcct ccttctcacc ttgctcttta taattattgc cgtagcgata gggatttatt
     1921 ggtttttggt actgcgtcat ttcgaagaaa ccgatgacgc atacgtggca gggaatcaga
     1981 ttcaaattat gtctcaggtg tctggcagcg tgacgaaagt ctgggccgat aacaccgatt
     2041 ttgtaaaaga aggcgatgtt ctggtcactc tcgacccgac agatgctcgc caggcgtttg
     2101 aaaaagccaa aactgcactg gcttccagcg ttcgccaaac ccaccagctg atgattaaca
     2161 gcaaacagtt gcaggcgaat attgaggtgc aaaaaatcgc cctcgcgaaa gcacaaagcg
     2221 actacaaccg ccgtgtgccg ctgggcaatg ccaacctgat tggtcgcgaa gagctgcaac
     2281 acgcccgcga cgccgtcacc agtgcccagg cgcaactgga cgtcgctatt caacaataca
     2341 atgccaatca ggcgatgatt ctggggacta aactggaaga tcagcctgcc gtgcaacagg
     2401 ctgccaccga agtacgtaac gcctagctgg cgctggagcg tacccgtatt gtcagtccga
     2461 tgaccggtta tgtttcccgt cgtgccgtgc aaccgggtgc gcaaattagc ccaacgacgc
     2521 cgctgatggc ggtcgttcca gccaccaata tgtgggtgga tgccaacttt aaagagacgc
     2581 agattgccaa tatgcgcatc ggtcagccgg tcaccatcac caccgatatt tacggcgatg
     2641 atgtgaaata caccggtaaa gtggttggtc tggatatggg cacaggtagc gcgttctcac
     2701 tgcttccggc gcaaaatgcg accggtaact ggatcaaagt cgttcagcgt ctacctgtgc
     2761 gtatcgaact ggaccagaaa cagctggagc aatacccgct gcgcattggt ttgtccacgc
     2821 tggtgagcgt caataccact aaccgtgacg gtcaggtact ggcaaataaa gtacgttcaa
     2881 caccggtcgc ggtaagtacc gcgcgtgaaa tcagcctggc acctgtcaat aaactgatcg
     2941 acgatatcgt aaaagctaac gctggctaat ccagaggtgc gtgtgatgca acagcaaaaa
     3001 ccgctggaag gcgcgcaact ggtcattatg acgattgcgc tgtcactggc gacattcatg
     3061 caggtgctga actccaccat tgctaacgtg gcgatcccca ctatcgccgg gaatctgggc
     3121 tcatcgctca gccaaggaac gtgggtaatc acttctttcg gggtggcgaa tgccatctcg
     3181 atcccgctta ccggctggct ggcaaagcgc gtcggggaag tgaaactgtt cctttggtcc
     3241 accatcgcct ttgctattgc gtcttgggcg tgtggtgtct ccagcagcct gaatatgctg
     3301 atcttcttcc gcgtgattca ggggattgtc gccgggccgt tgatcccgct ttcgcaaagt
     3361 ctattgctga ataactaccc gccagccaaa cgctcgatcg cgctggcgtt gtggtcgatg
     3421 acggtgattg tcgcgccaat ttgcggcccg atcctcggcg gttatatcag cgataattac
     3481 cactggggct ggatattctt catcaacgtg ccgattggcg tggcggtggt gttgatgaca
     3541 ctgcaaaccc tgcgcggacg tgaaacccgc accgaacggc ggcggattga tgccgtgggg
     3601 ctggcactgc tggttattgg tatcggcagc ctgcagatta tgctcgaccg cggtaaagag
     3661 ctggactgtt tttcatcaca ggaaattatc attcttaccg tggtggcggt ggtggctatc
     3721 tgcttcctga ttgtctggga gctgaccgac gataacccga tagtcgatct gtcgttgttt
     3781 aagtcgcgca acttcaccat cggctgcttg tgtatcagcc tcgcgtatat gctctacttc
     3841 ggcgctattg ttctgctgcc gcagttgttg caggaggtct acggttacac ggcgacctgg
     3901 gcaggtttgg cctctgcgcc ggtagggatt attccggtga ttctgtcgcc gattatcggc
     3961 cgcttcgcgc ataaactgga tatgcggcgg ctggtaacct tcagctttat tatgtatgcc
     4021 gtctgcttct actggcgtgc ctataccttt gaaccaggta tggatttttg cgcgtcggcc
     4081 tggccgcagt ttatccaggg gtttgcggtg gtctgcttct ttatgccgct gaccaccatt
     4141 acgctgtctg gtttgccacc ggaacgactg gcggcggcat cgagcctctc taactttacg
     4201 cgaacgctgg cggggtctat cggcacgtcg ataaccacga ccatgtggac caaccgcgag
     4261 tcgatgcacc atgcgcagtt gactgagtcg gtaaacccgt tcaacccgaa tgcccaggcg
     4321 atgtacagtc aactggaagg gcttgggatg acgcaacagc aggcatcagg ctggattgcc
     4381 cagcagatca ccaatcaggg gctgattatt tccgccaatg agatcttctg gatgtcagcc
     4441 gggatattcc tcgtactgct ggggctggtg tggtttgcta aaccgccatt tggcgcaggt
     4501 ggcggcggag gcggtgcgca ctaagtacaa ctaagccagt tcatttgaac tggctttttt
     4561 caattaattg tgaagatagt ttactgacta gatgtgcagt tcctgcaact tctctttcgg
     4621 cagtgccagt tcttcgttgc tgttgatgcg tacctcacgt tccagaatgc tacgcgcaat
     4681 atcctgcgct tcctgcaacg agtgcatctg gtaagtgcca cactggtaga cgttcagttc
     4741 cgggatctga ttctgatcct gcactttcag cacgtcttcc attgccgctt tccaggcatc
     4801 agcaacacgc tgctcatctg gcgtaccaat cagactcata taaaaaccgg tgcggcagcc
     4861 cattggcgag atatcgataa tctctacacc attaccgtta agatggttac gcataaaacc
     4921 agcaaacagg tgctccaggg tatggatccc tctttctggc atcacttctt tgttcggcac
     4981 gcagaagcgc agatcgaaca cggtgattgc gtcgccatgc ggggtgttca ttgttttcgc
     5041 cacccgaact gcaggcgctt ccatccgggt atgatcgact gtgaagctat ctaacaacgg
     5101 catttagcca cctccggtaa tttttttaaa aattttctga actctttctt cccaggcgag
     5161 tctgagtata tgaaagacgc gcatttgtta tcatcatccc tgaattcaga gatgaaattt
     5221 tggccactcc cgagtggcct ttttcttttc tgtcaggcgt gtttttccag ccacaccgca
     5281 aacggttcgg tatcagcggt ttccatttcc tgctgacggc gttcagatgc ctcgcgctcg
     5341 gctacaaaat cctcttcgcg cagaatttcc agcggctctt cacgcagcag attacggtag
     5401 gcttctgcaa acgctttgcc tgttccgcca ataccagtat caatcataga ccttaagata
     5461 cgggcagaga aagtcagatc gggattatcg aagcaggcaa ccagttcatc acacactttc
     5521 tgatacgctt cgccgccgtt aatgctatcc agcgtttgcg cgacgcgttt cagatcgcgg
     5581 aacagatctt tacccacctg cagtaacggg aactgcgcgg tttcgcagcc gatacccagc
     5641 gtcaggcccg gtttgcgacc ttcaaggatc acccggttcc agttaacgcg cgtacaggca
     5701 agttcgctac tgctcatttc cggtgcatca gccagcgcac accagaccat aaacaggtcg
     5761 aggaatcgca cctgctgttc atctacacca atcggcgaga acgggttgat gtccagcgaa
     5821 cgcacttcaa tatattcaat gccgccacgt aacagcgcat cagaaggtga ctcgccgctg
     5881 cgggtaacgc gttttggacg aatcggcgca tacagttcgt tttcaatctg cagcacgttg
     5941 ctattgattt gcagcctctt accgtctttc tcaataccaa tcttcgcgta ctcttccgat
     6001 ggcgttttga ttgcctgttt aaggcccgct acgtactcgt aaagatcgtt gaaggtaata
     6061 ccaagattgc tttgcgattt attggtatag ccgagatcgc tcaaacgaag agaggtcgca
     6121 tacggcaggt aatacatacc gcactcggtt ttctcaaacg gcagcgacgt tggttttcct
     6181 tgcaggaaag aagaacaaat cgccggagat gcaccaaaca gataaggaat aacccaaccg
     6241 aaacgatagt aattgcggat aacgcggaaa tagcccgcag aaattttctc tttggcatca
     6301 gcgcccgaga tatcaccgca cttcgcttgc cagaatgcca ttggcaaaga gaaattgtag
     6361 tgcacgccgg aaatggtttg catcagcgcg ccgtagcgat ttttcagccc ttcacgatac
     6421 agcgttttaa agcgtccggt gttagaagtg ccgtactgtg ccagttcgat gtcctgacct
     6481 tctgcgatgt agcatggcat acttaacggc cacatccgct catcgcccat attgcgcgcc
     6541 gtataacgat gcagatcgcg cataaaggtc agcatatgtt caatatcacc atccactggt
     6601 gtaatgaatt ccagcaatgc ttccgcaaaa tcggtagtaa tccatttgtg cgtcagtgcg
     6661 gaacctaatg cttcaggatg acctgttgtt gccagtgtgc catcagcatt aacacgcaaa
     6721 gtttcgcgct ccagcccacg ctgtatcccc tttaacgcct gaggatgttt ttccagccag
     6781 gccagcgcct gtgatacgtc cgggatcaaa ttgacctccc gcctgtcaaa atcgttttaa
     6841 ttagcataac tgtaatggtg accatatgtg caggcctaca attagtgcca ccacatcatg
     6901 ccctgaacgg tcgctgctgc aactgcaaca tagcgtaacg ctttaccaag gcacaaaaaa
     6961 aagattactg gcccccagga gatgcgcatc catcccgcta acagacacag taaatcgcca
     7021 accacgggca tccagcttaa taatagcgtg actgcaccat agcgtttcag ccagccggta
     7081 gctttctctt gccagcgcga tgttttacgc aatggaaaga aacgcccaag gataacgtta
     7141 gttaaccctc caaggctatt acccattgtt gctgttaaaa ctaaaaccca gggatgactg
     7201 atcccggaaa gcaacattgc caccagcacg acttcggagt tgccgggtaa tagtgtagcg
     7261 ctgagaaaac tactggcaaa caacgagaaa agcgataacg cttcactcac agcaagcgaa
     7321 catccacagc gtccatgcct gctgcacggg ccgcctgaat accgaaatcg gcatcttcaa
     7381 agaccacaca ctgcgtcggt tgcacgccca tacgctgcgc gcacaacaaa aatgtgtctg
     7441 gcgcgggttt atggtgtttg acgtgatcag cagcgacgac ggcgtcaaaa taacggcgta
     7501 atcccaggtg cgccagcaat gcctcagcga tggcgctttc actccccgtt cctacagcca
     7561 ttgggcgacg accatgccaa cttttcacca cttcaacaag aggaagcggc tcgacactat
     7621 ccagcagcat acttcttact gcttctgttt tttcacgcgc taacgcatgc gggtcgagat
     7681 cggcctgatt cagctcaata attgcctgag caatacgcca ggtgggcgat ccattaagcg
     7741 caatcatcgc ctgaatatcg tactgaagac cgtagtgccc taatacttcg cgccacgctt
     7801 tacggtgcgt aggcgccgta tccaggattg tgccatccat atcaaaaatt aaacctgcat
     7861 aacgctcgta catggtcttc tcgcaagtcg aaaaatatga cgctacttta gcgtaattgc
     7921 ttgattttgt cgctgatggc gaaggagaag aaaattcagg aaatggataa agtagttatt
     7981 tagaaatgag atatttttga aggaaatttt ttggagaata tggtgcatcc gggaggattc
     8041 gaacctccga ccgctcggtt cgtagccgag tactctatcc agctg
//
LOCUS       DAZSNM010000182         7881 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZSNM010000182 DAZSNM010000000
VERSION     DAZSNM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7881
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.182"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="ACHW42_003330"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="ACHW42_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHW42_003331"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHW42_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR8945015.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHW42_003332"
     CDS             complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHW42_003332"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR8945016.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHW42_003333"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHW42_003333"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR8945017.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="ACHW42_003334"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="ACHW42_003334"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR8945018.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7783..7858
                     /locus_tag="ACHW42_003335"
     tRNA            7783..7858
                     /locus_tag="ACHW42_003335"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7816..7818,aa:Gly,seq:gcc)
BASE COUNT         1785 a         2041 c         2134 g         1921 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagcac
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagata
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat agctcagttg
     7801 gtagagcacg accttgccaa ggtcggggtc gcgagttcga gtctcgtttc ccgctccaaa
     7861 atttaaaagt gctgtaaggc a
//
LOCUS       DAZSNM010000183         7857 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZSNM010000183 DAZSNM010000000
VERSION     DAZSNM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7857
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.183"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            17..817
                     /gene="yigL"
                     /locus_tag="ACHW42_003336"
     CDS             17..817
                     /gene="yigL"
                     /locus_tag="ACHW42_003336"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR8945019.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            893..1792
                     /gene="bioP"
                     /locus_tag="ACHW42_003337"
     CDS             893..1792
                     /gene="bioP"
                     /locus_tag="ACHW42_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR8945020.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(1680..2633)
                     /gene="metR"
                     /locus_tag="ACHW42_003338"
     CDS             complement(1680..2633)
                     /gene="metR"
                     /locus_tag="ACHW42_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR8945021.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            2869..5130
                     /gene="metE"
                     /locus_tag="ACHW42_003339"
     CDS             2869..5130
                     /gene="metE"
                     /locus_tag="ACHW42_003339"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR8945022.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(5169..5984)
                     /locus_tag="ACHW42_003340"
     CDS             complement(5169..5984)
                     /locus_tag="ACHW42_003340"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR8945023.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            6246..7007
                     /gene="udp"
                     /locus_tag="ACHW42_003341"
     CDS             6246..7007
                     /gene="udp"
                     /locus_tag="ACHW42_003341"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR8945024.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            7148..>7857
                     /gene="rmuC"
                     /locus_tag="ACHW42_003342"
     CDS             7148..>7857
                     /gene="rmuC"
                     /locus_tag="ACHW42_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR8945025.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARS"
BASE COUNT         1796 a         2005 c         2177 g         1879 t
ORIGIN      
        1 gaggtttaaa tttcttatgt accaggttgt tgcgtctgat ttagatggca cgttactttc
       61 tcccgaccat acgttatccc cttacgccaa agaaactctg aagctgctca ccgcgcgcgg
      121 catcaacttt gtgtttgcga ccggtcgtca ccacgttgat gtggggcaaa ttcgcgataa
      181 tctggagatt aagtcttaca tgattacctc caatggtgcg cgcgttcacg atctggatgg
      241 caatctgatt tttgctcata acctggatcg cgacattgcc agcgatctgt ttggcgtagt
      301 caacgacaat ccggacatca ttactaacgt ttatcgcgac gacgaatggt ttatgaatcg
      361 ccatcgcccg gaagagatgc gcttttttaa agaagcggtg ttcaaatatg cgctgtatga
      421 gccgggatta ctggagccgg aaggcatcag caaagtgttt ttcacctgcg attcccatga
      481 acaactgctg ccgctggagc aggcgattaa cgctcgctgg ggcgatcgcg tcaacgtcag
      541 tttctctacc ttaacctgtc tggaagtgat ggcgggcggc gtgtcgaaag gccatgcgct
      601 ggaagcagtg gcgaagaaac tgggctacag cctgaaggat tgtattgcgt ttggtgacgg
      661 gatgaacgac gccgaaatgc tgtcgatggc ggggaaaggc tgcattatgg gcagtgcgca
      721 ccagcgtctg aaagatctgc atccggagct ggaagttatt ggtactaatg ccgacgacgc
      781 ggtgccgcat tatctgcgta aactctattt atcgtaatcg ttctttattt ggtcagttgt
      841 caacctgata cttcgctaca atggatactc gttaatcaaa gagttttcca ttgtggcgtt
      901 acttatcatc accacgattc tgtgggcctt ctcctttagc ttttatggcg agtaccttgc
      961 ggggcacgtc gatagctatt ttgcggtgct ggtgcgcgtt ggcttggcgg cactcgtttt
     1021 tctgccgttt ctgcgtaccc gtagcaatag cctgaaaacg gtcggcctgt atatgctggt
     1081 gggcgcgatg cagctcggcg tgatgtatat gctgagtttc cgcgcttatc tctacctgac
     1141 ggtctccgaa ttgttgctgt tcaccgtact gacgccgctc tacatcacgc tgatttatga
     1201 catcatgagt aagcgccgtc tgcgctgggg ctatgctttt agcgccttgc tggcggtgat
     1261 tggtgccggg attattcgct atgatcaggt caccgaccat ttctggactg gcttgttgct
     1321 ggtgcaactc tccaatatca cttttgccat cggcatggtg ggttacaaac gcctgatgga
     1381 aactcgcccg atgccgcagt ataacgcctt tgcgtggttc tatcttggcg cgttcctggt
     1441 ggcggtaatt gcatggttct tgctgggaaa tgcgcagaaa atgccgcaaa ccacgttgca
     1501 atggggcatt ctggtgtttc ttggcgtggt ggcttccggg attggctact ttatgtggaa
     1561 ctacggcgcg acgcaggtgg acgccggaac gctgggcatt atgaataata tgcacgttcc
     1621 ggcagggctg ctggtaaacc tggctatctg gcaccaacag ccgcactggc caacgtttat
     1681 tacaggcgcg ctggtgatcc tggcctcact gtgggtgcat cgtaagtggg tcgctccgcg
     1741 ctcttcacaa acggcagatg atcgcaggcg tgattgcgcg ctgagcgaat aaacgcttcc
     1801 gtaactggct gacgctgctc gccatcgcgc acggcggcgt acagtcggct ccacaagcct
     1861 tcgcccaggg ttttggtcac caccagaccc tggcgctcaa aactctctac tacccaatgc
     1921 ggtagcgcgg caatacccat ccgcgcggca accatttgaa tcaacaataa ggtgttatcg
     1981 acgcttttca gtgacgggct gacgcctgcc ggctgaagaa aatgccgcca gacatccagt
     2041 cgactacgct gcaccggata aattaatagc gtctcgctgg cgagatcttc cggtgtaata
     2101 cgcgtttttg ccgccagtgg atggtcaggt gctaacacca ggcgtacttc ataatcgaac
     2161 atcggcgaat aatgcaggcc actgcgcggc aggatatcgg aggtcatcac cagatccagc
     2221 tcgccctgtt gcaacgcggg ctgcgggtca aacgtcacgc ccgatttaaa atccatctct
     2281 acctgcggcc agttcttatg gaaatttcct aaagcgggtg tcagccactg aatacagcta
     2341 tggcactcaa tggcaatgcg cagacgcgtc tgctgcggtt cattgcaggc ttgcagtgcc
     2401 tggctgattt gcggcagcac ctggtttgcc agttgcaaca ggatttctcc ctgcggtgta
     2461 aagcgtagcg gctggctctt acgcacaaat agccggaagc caaggcgttg ttccagatcg
     2521 ctaaactggt gagacagggc ggattgcgtc tgatgcaacg ttgccgcagc ggctgcgagc
     2581 gagccgcagt tccgcaacgc ttgtagcgtt ttcaggtgtt ttacttcgat catgaaagtc
     2641 cttcacttcg gcatgaataa tttgcgcttg aggaatatac agtaaccgcc aattatggat
     2701 gtgtaaacat ctggacggct aaaatcattc gtcttttaaa tttatggtgc gttggctgcg
     2761 tttctccacc ccggtcactt acttcagtaa gctcccggcg atgaataaac ttgccgcctt
     2821 ccctaaattc aaaatccata ggatttacat ataattagag gaagaaaaat gacaatattg
     2881 aatcacaccc tcggtttccc tcgcgttggc ctgcgtcgcg agctgaaaaa agcgcaagaa
     2941 agttattggg cggggaactc cacgcgtgaa gaactgctga cagtagggcg tgaactgcgt
     3001 gctcgtcact gggatcaaca aaagcaagcg ggtatcgacc tgctgccggt gggcgatttt
     3061 gcctggtacg atcatgtact gaccaccagt ttgctgctgg gtaatgttcc gccacgtcat
     3121 cagaacaaag acggttcggt agatatcgac accctgttcc gtattggtcg tggacgtgcg
     3181 ccgactggcg aacctgcggc ggcagcggaa atgaccaaat ggtttaacac caactatcac
     3241 tacatggtgc cggagttcgt taaaggccaa cagttcaaac tgacttggac gcagctgctg
     3301 gaggaagtgg acgaggcgct ggcgctgagc cacaacgtta agcctgtgct gctggggccg
     3361 gttacttacc tgtggctggg gaaagtgaaa ggtgaacagt ttgaccgcct tagcctgctg
     3421 aacgacattc tgccggttta tcagcaagtg ctggcagaac tggcgaaacg cggcatcgag
     3481 tgggtacaga ttgatgaacc cgcgctggta ctggaactac cacaggcgtg gctggacgca
     3541 tacaaacccg cttacgacgc gctccaggga caggtgaaac tgctgctgac cacctatttt
     3601 gaaggcgtaa cgccaaatct cgacacgatt actgcgctgc ctgttcaggg gctgcatgtg
     3661 gaccttgtac atggtaaaga taacgttgtt gaactgcaca agcgcctgcc ttctgactgg
     3721 ctgctgtctg cgggtctgat caatggtcgt aacgtctggc gcgccgatct tactgagaaa
     3781 tatgcgcaaa ttaaggacat tgtcggcaaa cgtgatttgt gggtggcatc ttcctgctcg
     3841 ttgctgcaca gccccatcga cctgagcgtg gaaacgcgtc ttgatgcaga agtgaaaagc
     3901 tggtttgcct tcgccctaca aaaatgccat gaactggcac tgctgcgcga tgcgctgaac
     3961 agtggtgaca cggcagctct ggcagagtgg agcgccccaa ttcaggcgcg tcgtcactct
     4021 actcgtgtac ataatccggc ggtagaaaag cgtctggcgg cgatcaccgc ccaggacagc
     4081 cagcgtgcga atgtctatga agtgcgtgct gaagcccagc gtgcgcgttt taaactgcca
     4141 gcgtggccga ccaccacgat tggttccttc ccgcaaacca cggaaattcg taccctgcgt
     4201 ctggatttca aaaagggcaa tctcgacgcc aacaactacc gcacgggcat tgcggaacat
     4261 atcaagcagg ccattgttga gcaggaacgt ttgggactgg atgtgctggt acatggcgag
     4321 gccgagcgta atgacatggt ggaatacttt ggcgagcacc tcgacggatt tgtctttacg
     4381 caaaacggtt gggtacagag ctacggttcc cgctgcgtga agccaccgat tgtcattggt
     4441 gacattagcc gcccggcacc gattaccgtg gagtgggcga agtatgcgca atcgctgacc
     4501 gacaaaccgg tgaaagggat gctgacgggg ccggtgacca tactctgctg gtcgttcccg
     4561 cgtgaagatg tcagccgtga aaccatcgcc aaacagattg cgctggcgct gcgtgatgaa
     4621 gtggccgatc tggaagccgc tggaattggc atcatccaga ttgacgaacc ggcgctgcgc
     4681 gaaggtttac cgctgcgtcg tagcgactgg gatgcgtatc tccagtgggg cgtagaggcc
     4741 ttccgtatca acgccgccgt ggcgaaagat gacacacaaa tccacactca catgtgttat
     4801 tgcgagttca acgacatcat ggattcgatt gcggcgctgg acgcagacgt catcaccatc
     4861 gaaacctcgc gttccgacat ggagttgctg gagtcgtttg aagagtttga ttatccaaat
     4921 gaaatcggtc ctggcgtcta tgacattcac tcgccaaacg taccgagcgt ggaatggatt
     4981 gaagccttgc tgaagaaagc ggcaaaacgc attccggcag agcgcctgtg ggtcaacccg
     5041 gactgtggcc tgaaaacgcg cggctggcca gaaacccgcg cggcactggc gaacatggtg
     5101 caggcggcgc agaatttgcg tcgggggtaa atccaaaccg ggtggtaata ccacccggtc
     5161 ttttctcatt acagcgactt cttcccacca tactgcttaa accattccag catacgctgc
     5221 cagccatctt tcgcagattc ggcatgatag ctcgggcgat aatcagcgtt gaatgcatgc
     5281 ccggcatccg ggtacacgat aatctctgct ttcgcattag cagcccgcag cgcctggcgc
     5341 atcgtttcaa cgctctcctg cggaatgctg ttatcctgac caccatataa gccgagaacc
     5401 ggcgcgttaa gatcggttgc gatatcaaca ggttgtttcg gtgaattcag cgacttgtcg
     5461 cccgtcagtt tgccgtacca cgccactgcg gcttttagct gtggattatg cgcggcatac
     5521 agccaggtga tacgtccacc ccagcagaat ccggtgatca ttaaacgatg aacatcgccg
     5581 ccgttgcggg aagcccaact ggcgacatga tcgagatcgg ccagcacctg cgagtcaggc
     5641 acttttgcta ccagaccgct aagcaacgtg gggatatcgg caaaatcatt cggatcgcct
     5701 tcgcggaagt aaagttcagg tgcgatagcc agatacccct ccagcgccag acggcgacaa
     5761 atgtcgcgga tatgttcatg cacgccaaaa atttcctgca ctacaatgac cactggcagt
     5821 gggccatcgc tttgctttgg tctggcatgg taagcaggca tgttatcccc ttgtgaaggg
     5881 atagaggtga agcccgccac aatcgcgtcg tccggggtct gaacgatggt cgaagcgaga
     5941 ggcgatgcag caggtgcaaa tccagattgt tgtgttgttg ccatggtatt ctccgtaccc
     6001 ttataaaaat gttgcgcaat gttaactata gtcagcatgc aacaaatcac attgcctgaa
     6061 tcggctcatc ttttatgcag tcctgcagaa tgaagagtga tttatgtgat atgaatcact
     6121 tttggtgggt aaatttatgc aacgcatttg cgtcatggtg atgagtatca cgaaaaaatg
     6181 ttaaaccctt cggtaaagtg tctttttgct tcttctgact aaaccgattc acagaggagt
     6241 tgtatatgtc caagtctgat gtttttcatc tcggcctcac taaaaacgat ttacaagggg
     6301 ctacgcttgc catcgtccct ggcgacccgg atcgtgtgga aaagatcgcc gcgctgatgg
     6361 ataagccggt taagctggca tctcaccgcg aattcactac ctggcgtgca gagctggatg
     6421 gtaaacctgt tatcgtctgc tctaccggta tcggcggccc gtctacctct attgctgttg
     6481 aagagctggc acagctgggc attcgcacct tcctgcgtat cggtacaacg ggcgctattc
     6541 agccgcatat taatgtgggt gatgtcctgg ttaccacggc gtctgtccgt ctggatggcg
     6601 cgagcctgca cttcgcaccg ctggaattcc cggctgtcgc tgatttcgaa tgtacgactg
     6661 cgctggttga agctgcgaaa tctattggcg cgacaactca cgttggcgtg acagtttctt
     6721 ctgatacctt ctacccaggt caggaacgtt acgatactta ctctggtcgc gtagttcgtc
     6781 actttaaagg ttctatggaa gagtggcagg cgatgggcgt aatgaactat gaaatggaat
     6841 ctgcaactct gctgaccatg tgtgcaagtc agggcctgcg tgccggtatg gtagcgggtg
     6901 ttatcgttaa ccgcacccag caagagatcc cgaatgctga gacgatgaaa caaaccgaaa
     6961 gccatgcggt gaaaatcgtg gtggaagcgg cgcgtcgtct gctgtaattc tcttctcctg
     7021 tctgaaggcc gacgcgttcg gccttttgta tttttgcgta gcgcctcgca ggaaatgcct
     7081 ttccaactgg acgtttgtac agcacaattc tattttgtgc gggtaagttg ttgcgtcagg
     7141 aggcgttgtg gatttctcaa tcatggttta cgcagttatt gcgttggtgg gtgtggcaat
     7201 tggctggctg tttgccagtt atcaacatgc gcagcaaaaa gccgagcaat tagctgaacg
     7261 tgaagagatg gtcgcggagt taagcgcggc aaaacaacaa attacccaaa gcgagcactg
     7321 gcgtgcagag tgtgaattgc tcaataacga agtgcgcagc ctgcaaagta ttaacacctc
     7381 tctggaggcc gatctgcgtg aagtaaccac gcggatggaa gccgcacagc aacatgctga
     7441 cgataaaatt cgccagatga ttaacagcga gcagcgcctc agtgagcagt ttgaaaacct
     7501 cgccaaccgt atttttgagc acagcaatcg ccgggttgat gagcaaaacc gtcagagtct
     7561 gaacagcctg ttgtcgccgc tacgtgaaca actggacggt ttccgccgtc aggttcagga
     7621 cagcttcggt aaagaagcac aagaacgcca taccctgacc cacgaaattc gcaatctcca
     7681 gcaactcaac gcgcaaatgg cccaggaagc gatcaacctg acgcgcgcgc tgaaaggcga
     7741 caataaaacc cagggcaact ggggcgaggt agtattgacg cgggtgctgg aggcttccgg
     7801 tctgcgtgaa gggtatgaat atgaaaccca ggtcagcatc gaaaatgacg cccgctc
//
LOCUS       DAZSNM010000184         7846 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZSNM010000184 DAZSNM010000000
VERSION     DAZSNM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.184"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..593
                     /gene="minD"
                     /locus_tag="ACHW42_003343"
     CDS             <1..593
                     /gene="minD"
                     /locus_tag="ACHW42_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR8945026.1"
                     /translation="IKDKRTENLYILPASQTRDKDALTREGVAKVLDDLKAMDFEFIV
                     CDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGILASKSRRAENGEEPIKE
                     HLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVLRASNQGEPVILDINAD
                     AGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            597..863
                     /gene="minE"
                     /locus_tag="ACHW42_003344"
     CDS             597..863
                     /gene="minE"
                     /locus_tag="ACHW42_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR8945027.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1235..1579)
                     /locus_tag="ACHW42_003345"
                     /pseudo
     CDS             complement(1235..1579)
                     /locus_tag="ACHW42_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1614..1733)
                     /locus_tag="ACHW42_003346"
     CDS             complement(1614..1733)
                     /locus_tag="ACHW42_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR8945028.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1694..1879)
                     /gene="ymgJ"
                     /locus_tag="ACHW42_003347"
     CDS             complement(1694..1879)
                     /gene="ymgJ"
                     /locus_tag="ACHW42_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR8945029.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            1980..2153
                     /gene="ymgI"
                     /locus_tag="ACHW42_003348"
     CDS             1980..2153
                     /gene="ymgI"
                     /locus_tag="ACHW42_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR8945030.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            2155..2499
                     /locus_tag="ACHW42_003349"
     CDS             2155..2499
                     /locus_tag="ACHW42_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR8945031.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            2503..2838
                     /locus_tag="ACHW42_003350"
     CDS             2503..2838
                     /locus_tag="ACHW42_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR8945032.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(2895..5216)
                     /locus_tag="ACHW42_003351"
     CDS             complement(2895..5216)
                     /locus_tag="ACHW42_003351"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR8945033.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(5333..5542)
                     /locus_tag="ACHW42_003352"
     CDS             complement(5333..5542)
                     /locus_tag="ACHW42_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945034.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(5942..6161)
                     /gene="ymgF"
                     /locus_tag="ACHW42_003353"
                     /pseudo
     CDS             complement(5942..6161)
                     /gene="ymgF"
                     /locus_tag="ACHW42_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
     gene            complement(6293..7816)
                     /locus_tag="ACHW42_003354"
     CDS             complement(6293..7816)
                     /locus_tag="ACHW42_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR8945035.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2190 a         1839 c         1675 g         2142 t
ORIGIN      
        1 taattaaaga taagcgtact gaaaatctct atattcttcc ggcatcgcaa acacgcgata
       61 aagatgccct cacccgtgaa ggggtcgcca aagttcttga tgatctgaaa gcgatggatt
      121 ttgaatttat cgtttgtgac tccccggcag ggattgaaac cggtgcgtta atggcactct
      181 attttgcaga cgaagccatt attaccacca acccggaagt ctcctcagta cgcgactctg
      241 accgtatttt aggcattctg gcgtcaaaat cacgccgcgc agaaaatggc gaagagccta
      301 ttaaagagca cctgctgtta acgcgctata acccaggccg cgtaagcaga ggtgacatgc
      361 tgagcatgga agatgtgctg gagatcctgc gcatcaaact cgtcggcgtg atcccagagg
      421 atcaatcagt attgcgcgcc tctaaccagg gtgaaccggt cattctcgac attaacgccg
      481 atgcgggtaa agcctacgca gataccgtag aacgtctgtt gggagaagaa cgtcctttcc
      541 gcttcattga agaagagaag aaaggcttcc tcaaacgctt gttcggagga taagttatgg
      601 cattactcga tttctttctc tcgcggaaga agaacacagc caacattgca aaagaacggc
      661 tgcagattat tgttgctgaa cgccgtcgca gcgatgcaga accgcattat ctgccgcagt
      721 tgcgtaaaga tattcttgag gtcatttgta aatacgtaca aattgatcct gagatggtaa
      781 ccgtacagct tgagcaaaaa gatggcgata tttctattct tgagctgaac gtgaccttac
      841 cggaagcaga agagctgaaa taagcccgct gtaaaaacgc atttatcttc aaggcagagt
      901 catttctgcc ttgagttttt catccctctc atccacgttg tggtaaagcg gcgagtattc
      961 ttgctgatac tcctcattgc tatttccacc tcccccttta caccttaagg ctgttcccac
     1021 gcacagaaca aaatgtttat gcctggttga gtaaataacc atgttattag tgtgtatata
     1081 ctgggagatg ataaccccgc aatttcatta ttcattgttc tcccatagga tgaggtttcc
     1141 cgccgtaact ggcgggcttt ttttgcccca gaattttctc tttcaacatc ctgtaaacga
     1201 aaactgcgcc gaagcgcagt ttaatcgttg atggtcagaa cgaccagttc acaccagcca
     1261 ccgcgttcca cggggactcc acaccggcac catggctata ccccaccccg aaatgcccgc
     1321 ttaacgtact gctgaatgag gctttaatac ctgcctggta tattccacgt ctgcccgaca
     1381 aatcattgac aaaattaccg tcactattca ctttcacccg attatcatcg acaaattctt
     1441 tgcgcacagc cgccttcagg cagggttcaa tttccatacc gttccccaga cgcatgttgt
     1501 aactcagcgt tgcacccagt tcacgatata tactgcgggt atcgactgat ttcgattcca
     1561 tgccattgga taaatgatag tagcgtctaa gtgacctaag ctctgctgca acatcagccc
     1621 gcaggcacca gaccagggga ttcatcctga agagacagcg caggtgtatt gtgttcaccg
     1681 ctcaccaaag acatcatgat gaaatgatga tatcccgcat aagaaagagg cattttttac
     1741 acgcagtgcg ctgaagtgta gttggataaa aaatcactct tttcagataa tactgtcgta
     1801 cgcttatctt tagacaatga ggccgtcgga aaaataaaat gactaatgta atcgttgctg
     1861 atgaatatct tctgctcaca taaaaatcac acaataatat tgataaatca gattatctta
     1921 cctttacaac attgcgttaa cagtcaatga cacttcttga ataaaaatat tgtcaatcaa
     1981 tgagttattc ctcatttaag attattctca ttcacgtaaa aaacataatt cctatcatca
     2041 cagccacgct cattttgaat tatctgaata atagcgagcg ttctttagtt aagcagatct
     2101 taattgaaga cgaaattatt gtgtgtgcaa cttacttaat accggacatc taagttgaag
     2161 aaaaaaatac ttgccttcgg gcttatctca gcattgttct gctccacgcc agcgatagcg
     2221 gatatgaacc gcaccacgaa aggtgcattg ctaggcgcag gcgttggttt acttactggc
     2281 aatggtgtta atggcgtact gaaaggtgca gctgttggcg ctggtgttag tgcagtaaca
     2341 gaaaaaggcc gcgacggtaa aaatgcgcgt aaaggtgcaa aagtgggtgc cgccgttggt
     2401 gctgtcactg gcgttctgac cggcaatggc ctcgaagggg cgattaaagg tgccgtgatt
     2461 ggcggtacgg gtggtgcaat tctgggtaaa atgaaataag gaatgatgat gaaaaaattt
     2521 gctttactgg cgggcctgtt tgttttcgca ccaatgacct gggcgcagga ctacaatatt
     2581 aaaaacggtt tgccgtcaga aacctatatt acttgcgcag aagccaacga aatggcaaaa
     2641 actgacagcg cacaggttgc agaaattgtt gcggtaatgg gtaatgccag cgttgccagc
     2701 cgtgatttaa aaattgagca atctccggaa ctgagcgcca aagtggtaga aaaactgaat
     2761 caggtttgcg ctaaagaccc gcaaatgctg ttgatcacgg caattgatga caccatgcgt
     2821 gctatcggca aaaaataata tttcatatgt accgcgtcga tgaatacgac gcggtttttt
     2881 agctaacggc gggatcagaa cttatatttc aggcccacca tcactgcggt gtcattgtag
     2941 ccattatcac ccagctgcac gcccacattc ccccagacgc tggccgctgg attcaggttg
     3001 ccatttacgc caagtttaat ttcccccaaa ttactcaccc catcttgttc gactttcaca
     3061 ccgttcattg agacagcaaa atctttactg ttatggatcc agttcatttc aacaaaaggt
     3121 tcaaagtcgt caccgtgcgg accgctgttt ttctcctgag tacgaataaa ggtgcggaag
     3181 ccgagtcggg tctggatgtt gttatttgcg tcattctcaa tacgtgagcc attggcttcc
     3241 gtgtgcgcat tatgatcgac gcccatccag atagcctgtg cctgtggctg gaagatccac
     3301 gtatatttcg ccgcttccag accaaaggat ttattcaggg caaaaccgta acccgcttcc
     3361 agcgagacgg ttgcaccttt cgcgctatag gattcatagg ctaattcatc ccctttcacg
     3421 gagtttttaa accagctata ttgcgcccag gagtcaatgt atgcgccttt tttactgatg
     3481 tcatcggcaa accaagtggc atacagccct gcgctatagc ctctgacact ccctttcgaa
     3541 cgataatccg acacgctgga atgagttaag ttgtagtcgc gggcataacc agccatcact
     3601 cccaaacgcc aactgtcact atcggtaaaa ccaccggtta acagatcgcc ccctaactgc
     3661 gagacgtagc gatgcgaggt cgttctcaac tgtccgttgc tgtcacgcca ggcattatgc
     3721 ccgccaattt gacgtagcca aagtcggctt gaacgctcct gttcagttac aggatcgatg
     3781 tagcgcgttt cacccgcacg gtcgttcaga cgcataacaa aaagagtgtt agcggctgcc
     3841 aggttggcga cgtaacttcc agcttcgggg cgcaggacgg gggtaggcgc aacagtaggc
     3901 gctgaacttc ctccggagtt tgtttcaccg ccgctcgttg cctggaagtt agtcagatac
     3961 cagtttttat tggtcgcttc ggtattacct cgggttaatg aatattcgta ggcaccggcg
     4021 aaaatcctcg aatccttaat aaattcccca tttgattccc cctcaacgct gataatctct
     4081 atcccattta acgtgtaagc tccgctgcct cctgcattgg taactttaac tcgggttgtt
     4141 ccagaggtat ttccattaat aacgacttta tccgtagctg aattatcatc atccaattca
     4201 gttctgagat gcaatacgcc attattacca acgtaatttt cagttattgt taatcttgtt
     4261 ggttcgacat cccgtccatc tgcccgggaa atatagaccg tactattatc aaccgtgagg
     4321 ttgctcaccg ttgagtcagc tttaatatcc caggtactat tatctgaaag cgacagatag
     4381 gtatgagtgt tatcatctgt tgaaatatta gctgaacccg ttatttttga attagttgca
     4441 tcgacggtca aatgaccttt aatattattg ataaaaacat ctgcagccga tgatacctca
     4501 ctatccgtaa tggtcacgtt actcgtctca tctttatggt agaggatttc atttgctgac
     4561 cgtagtgagc taccagataa aatatctacc gttgcttcct gaccatttat ggaagactcc
     4621 agcacaattc cagacccctg atgcgcgact gcagatgtat ttttcagcgt caccgtgccg
     4681 ccattataaa ttttagcaat cgcggcgtca ggacccgttg tataaatttc tccctggtta
     4741 aatgaaactg tcccctggcg ggcagcgatg gcatgagcat tgtccccatg cgtggtgata
     4801 tcaacatcgg ttaatgtaac gctggcattg gtataatcaa gcgtgtcacc agccttgacg
     4861 acagtgccat tagcgtacgc ggcataactt tctgttccgt gggttgtgat ctcccctttt
     4921 gccatatcca gttttgctat ggcattattt tcattacttc ctgtatcggc agtactttct
     4981 tcatcaagag aagatacaca cgcacacgcg cttccgggag atgcacacgc aagaacgccg
     5041 actgcgtgat tacccgcagt aacaataaat gtatcatctc cgtcagaaaa agagggggta
     5101 accgaaccgt cagagttgac tcttattatg gtgtcagtat tcaggtttcc actcgtttga
     5161 cttagcaaac caattgagta atcccccgtt gtgacaatcg tgtttttacc ctgcaaaata
     5221 acagcaccgc caaattcact tacgattgcg ctagctgtta atgctgggct tagaggacta
     5281 ttgtcagaag gatataaacc gcccgttgtt acaatgacgc cattgttaat ggttaactta
     5341 ctaccctctc ctgcaacgga tacccgtaac ttccattacc cgttgttata actagcgcat
     5401 tatcaatatc ttttactttt ccatctttta ttgccacagc atgtttatat tcatcgtgct
     5461 gaataaactg ttcagcatat gcattaccca ccgccagagc aataagtccc aaactaaacc
     5521 cggggagttt tttcagtttc atagattaac tatccattca ttggattaac aaacattcct
     5581 ggtcaaataa cctatgagct cagattacca aaacggaggg taaagacaaa aacaaaagca
     5641 caaaaaattt aaatagataa acagcgacaa tatataaatg ataaataagt cggcattatc
     5701 atttttcacc ttattttcat gacattgatc actttggggt gactattttg tttaacaaat
     5761 aactcttcga catcaaaatt attaatagtt gccatggtaa ttatttcggt gatatataaa
     5821 aacaactgtg ataacaccaa ggttgacaac tatttgcgca ataattcctt tccttcaatg
     5881 atccaggcgt tcactgccag caacccattc agccgcatac tgacagtgag aaactgaaag
     5941 attattgctg atccctgtgg ctaacgagtc tttctcttaa atcgattaac cagattgatc
     6001 gccagccaaa caagggcatt aagcaaattt atcgtcacaa gcacttctcc cagcccggta
     6061 ctgggttttg caataaggat gaccaggagg gtgatcagca taaatgcaat ggaaaagatg
     6121 atatagagag tgaaataatc cagattatta ctattattca tgctactaat ggcaatcctg
     6181 tcaaaacttt caaacaatta tagcaaacaa aataatgaac aatagtaaca agaggcaacg
     6241 agctaattag ctgtctataa ttacaccgca ctatggcctt actgatcaat tttcactcaa
     6301 ccacaacctt caccttcggt ttagaaagga taattttgac caggtcgatg tatgtaacag
     6361 gtttatagaa ataataaccc tgctgaaatg tgatattatt ttggttcaga tagccaagtt
     6421 gttctttcgt ttcgacacct tcagcgacga tactgatcga aagtttacgc gccagatcca
     6481 atacacaatc cagaattcgg gttgattctg ggtctgcgtt tacgcggcca acgaaactgt
     6541 gatcaatttt gatataatca atatgcaggt catgaagata agaaagccct gagtagccag
     6601 taccgaaatc atccagcgcg atgacaaaac cattttcatg cagaatgttc aaccgctgaa
     6661 ccagactttc atcaacattc aatggctcac gctcggtgac ttccagaaca aggtttaaat
     6721 cgcggcgggt aaaactgtca cggaaattta aacactcgtc gacaaacgtc ggcgaaataa
     6781 tatgcgaagc gctaaaatta attccaatat gaaaaccttc cggcagttta ctcgcgatag
     6841 cgttcatctg tctggcaacc tgattcatca ggctttgtgt aagcggaacg attaatccgg
     6901 atttttcagc aagtggaata aatgccgcgg gtgaaatata tccaccgtga ggttgtttcc
     6961 agcgggctaa cacctcaact ccccgcaatg tcccttcccg accatttacc acaggttggt
     7021 aaaaagggac gatctcccca ttatttattg cccgtcgcag gatctcttca ggcgttgcgc
     7081 ttttattgaa atatctatca agcaggaagg cagcggcgct ggcgattaac agaataaata
     7141 ttaaaatccc aaagccttta tcgataagtc tgtagaagct aaagagtggc ggcgggttaa
     7201 agataatagt gaaatgatat tttgtggagt gaaccttctc ggtataggga taatgtccat
     7261 taagcgtcat cacatcagcc gttggtccaa taatgtcatc cgccacgcgt aatacatacc
     7321 ttaccccttt caagggtaca ttaagcgccc cgcgaatatg ttgatcactt atcgtaacca
     7381 aaatgcgcgt atctgcaaat tggctctgat agagcaggat aggtaatcta ttaacggtgt
     7441 cgattgccgg agccaacagt aaattactgt ccgggacaac aggaatacga gacagcagga
     7501 cccgattccc aggcagggat gtacaccaga ctattccctg tttgagaatg atgattgtgc
     7561 gcaggtgagg tttcagagct gcttcagtgc caagctgata ttgctcctcg aggtcgcatt
     7621 ccttcccggc aatgttaacc gctgtttgag tcgcctgtga cgcttcttca agtatatgat
     7681 tgatattgtt ggcggcatat cttgctcccg ccaggtactc tgcttttgcg gaataccaga
     7741 gctggatgtt caaaatagcg acaccggtaa taaacaagca tatcgcaacg aggatgggta
     7801 tgagtgtatt gcgcatttgc ttgtcccggc caaagggttt aataca
//
LOCUS       DAZSNM010000185         7820 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZSNM010000185 DAZSNM010000000
VERSION     DAZSNM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.185"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(349..834)
                     /gene="sixA"
                     /locus_tag="ACHW42_003355"
     CDS             complement(349..834)
                     /gene="sixA"
                     /locus_tag="ACHW42_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR8945036.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(1037..3181)
                     /gene="fadJ"
                     /locus_tag="ACHW42_003356"
     CDS             complement(1037..3181)
                     /gene="fadJ"
                     /locus_tag="ACHW42_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR8945037.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3181..4491)
                     /gene="fadI"
                     /locus_tag="ACHW42_003357"
     CDS             complement(3181..4491)
                     /gene="fadI"
                     /locus_tag="ACHW42_003357"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR8945038.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4671..4955)
                     /locus_tag="ACHW42_003358"
     CDS             complement(4671..4955)
                     /locus_tag="ACHW42_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR8945039.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5327..6667
                     /gene="fadL"
                     /locus_tag="ACHW42_003359"
     CDS             5327..6667
                     /gene="fadL"
                     /locus_tag="ACHW42_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR8945040.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            7033..>7820
                     /gene="yfdF"
                     /locus_tag="ACHW42_003360"
     CDS             7033..>7820
                     /gene="yfdF"
                     /locus_tag="ACHW42_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFR8945041.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERL"
BASE COUNT         2075 a         2000 c         1782 g         1963 t
ORIGIN      
        1 acatataatg cgattggcat ttatccactt ttgacaatat aatatataaa cactatagcc
       61 agaatttaat caagtttcaa ttacatcctg cctgattatt caactctatt ttctctatta
      121 tctgtattaa taaatgacaa tgagtgtcaa acagatatta ttcatttcaa catgtaactt
      181 tgggcaaaat caagatacgc gtagaagaat aatttccgaa tgaatgtaga aagtgtttta
      241 tttatagata aggagagtct tcgatagtag tgaggttgac agatatccgc tgcctaatga
      301 gcaattatgc ccaatgaatc tacctcattg ggcataattt ggaacacatc agatagcttt
      361 tgccatcttc agattacacg ggctcatctg ccagttgaat gtaccgtttc cgctttcatc
      421 aagcgttacg ctggcaatgg ccgaggtggt aaacataggc ggcgtttcgc ccggacataa
      481 ctcggcaacc agatacccga ctaacggtaa gtgagagatc accagtaccg aagcaacacc
      541 ttcattggtc aacgcctgca aataggcact gaccagacca acatcgccgc agggcgttaa
      601 ctccggcaga acttctgcac tggaaggcag gttcagacaa tcccctactt cttccagtgt
      661 ttgctcggct cgcaggaacg ggctcaccag aacgcgttcg atttccactt tttgaccttt
      721 cagccagttc gccatcaggc gagattcgtc acaaccatta gtggtcagag gacgaaccga
      781 atcactggcg gaatcgaggg ctgcgtcgcc gtgacgcatg ataaaaactt gcatattgca
      841 ccgcttttgt taaccagatt cgcccgtttt ctttcatctg aaaccagaga aagaaaacgg
      901 tggccggcat tgtgccttat ccattcaccg aatgaaacgc tgttttttac ctcaatggcg
      961 taagtatagt caatatgcgt ttacattttg accaacactc cgccattcag cgcggattca
     1021 tatagctttg accttcttat tgcaggtcag ttgcagttgt tttccaaaaa ctttccccac
     1081 gcgcgcccat ctcgaccaaa cgctcgcaag gggtaaaacg ggaaccatac tgcgtggcca
     1141 gtcgttgcat tattgcaacc acttcgcccg cgccgagaga atcgatatag cggaacggtc
     1201 caccgagaaa tggcggaaaa ccaatgccaa ataccgcacc aatatccccg tcacgcacgc
     1261 tacggataac ctgctcatca acacaacgta ctgcttcatt cagcatcaac atcacacacc
     1321 gttcagcaac ctgcggtgcg gagagtcgcc cctgcccttg tgcgccaatg agcgggtaaa
     1381 tggcaggatc gacctgtttt ttgcttttac gccctttctg accataaaga tagaaacccc
     1441 gaccattttt tctgcctttg cgatcgtcgt tcaaaattga agaaacaaca tttgcaggcg
     1501 cgctaaaacg ttctccataa gcggcttcca gtacaggaat aattttagtc ccggtgtcga
     1561 ttcctacctc atccaaaagt tggattgggc ctaccggaaa accaaatttc actagcgcgg
     1621 catcaatgtg ctctacccgt tcaccttcgg tcagcatgcg gatagcttca ttaatgtaag
     1681 gcgctaagat gcgattgacg taaaacccgg ctttgtcacg cacgacaatg ggcgttttgc
     1741 cctgtttttt cgccagtttt accgtagtgg cgatggtttg cgccgatgta cccgcatgag
     1801 gaataatctc taccagcggc attttttcca ccggactgaa gaaatgcagg ccaataactt
     1861 gctcaggtcg cgcggcgtga gcggcgatat caccaatcgg taaagatgac gtgttcgaag
     1921 caaaaatggt atgagcggcg caattttgct ccacttccgc caccatctgt tgttttaatt
     1981 cgagattttc aaacaccgct tcaataatca gatcgcgatg ggcaaagccg cgatagtccg
     2041 tcgttccgga gattaatgcc agctgtttgt cacgttcgct ggctttgaga tgacgacggc
     2101 gaactttgcc ctccagctga tcccaactgt acttcagcgc atgatttatg ccctgcgggt
     2161 tgatatcttt aattctgacc ggaagccccg ctttacaagc agtgacataa gcaataccgc
     2221 cgcccatcaa gccaccacct aaaattccca cgctgtttaa tggcgcaggc ggtgcatcac
     2281 taccgggatc tttcttcacg tccgtactgg caaaaaagat actacgcaaa gcttgcgatt
     2341 gcggcgtcat cgccagttcg ccaaaggcgc gagcttcagc gtcatagccg ctgctggtgc
     2401 cctgcgctaa tcccgtttca acaacctcca ggatgcgttc tgtcgccgga taattgcctt
     2461 gagttttgtg ttctgttttc ttgccgacca ttttgaacag cagcgcacga cctaacggcc
     2521 ccgccagaat acgctcgcgt acaggtagag ggcgggaaga tgggcgatcc ttcttcgcca
     2581 gctcaacagc ggcttccagc aaaatggagt gcggaacaac gtcatccacc agccccagct
     2641 ttaatgcctg tttcgcccga agttgttttc cggtgagaat catctctaat gctgtactga
     2701 cgcctatcag acgcggtaaa cgctgggtgc cgcctgaacc gggtaacaat ccaagttgta
     2761 cttcaggcaa accgagcacc gttttaggat cgtcagtaca aacgcgaccg tggcacgcca
     2821 gcgccagctc cagcccaccg cccaggcaag cgccatgaat agccgcgata accggaatgg
     2881 gcaaagcatg aatctccgcc atcaactgtt ggccctgccg cgccagtgct tccgcttctt
     2941 gcgccgtttt gcagttgcca atcatgttga tgtctgctcc ggcgataaaa ttgtccggtt
     3001 tcgcggagat aaacaccacg ccgcgcaact ctttgttttc acggagttgc ttaataatgg
     3061 cgcgcacctg cgaggcaaat tccgccttca gggtattcat tttctcatcc ggtacgtcga
     3121 tggtgatgat agcaatgttg tccagacgaa cattaagagt aaacgctgat gccatttcca
     3181 ttattccgcc tccagaacca ttgccgcgcc aagcccaccg gcagcacagg cggtaactaa
     3241 accaaatcca ccgccgcgac ggcgaagttc atgcaatgtc tgggtaatca tccgcgcgcc
     3301 ggtcgccgcg aagggatgtc cgtaagcaat cgaaccgcca agcacgttaa atttgctatc
     3361 gtccacttcg ccagtggcat gtgcacgccc cagtacttca cgagcaaaac gttcactacc
     3421 cagcaactga atattcgcca gtgtctgagc tgcaaaggct tcgtgcatat cgatcaatgt
     3481 cagttcgctc atcgtcaaac cggcacgttc cagcgccagc ggtgttgacc aggctggacc
     3541 gagcaacatg tcctgccaga catcaatcgc agtaaatgcg tagctgcgca gataccccag
     3601 cggcaccagc cctaattctt tcgcccggga ttcagtcatc aggatcaccg ctgccgcgcc
     3661 atcggtcagc ggcgtactgt ttgccgccgt taccgttccg tgtttgcgat caaacgccgg
     3721 gcgcagcttt gcgtaatcgg caagcgagga attaccgcga atattgttgt cttcgacaag
     3781 cggttgttta taaggaggga taaaggcagt catcacctct tctttgagtt ttccgtctga
     3841 ccatgcctga gcggcacgct gatgcgaacg gtgcgctaat gcatcttgct gttctcgggt
     3901 gatgccgtag gttttcgcca tttgttcggc ggtgtcgccc atccgcaagc cagtagaata
     3961 ttctgctacc gcaggcggta cgggcattaa gtcgcgcaaa cgcaggcgag agaagagttt
     4021 caggcgctgg ctcatggttc gagctttgtt gacatcaacc agcacgtacg ccagtttttt
     4081 actgacgcca attggcaata cggaagagga atctgcccca ccggcaatcc ccgctcggat
     4141 agttcccgcc atcaggcttt ctgcgacgtt tgcaaccgct tggaaactgg tagcgcaagc
     4201 gcggctgacg ctgtaagcat cggtatgcac attcattccg gtaccgagaa caatttcacg
     4261 cgcaatgttg ggggcttcag gcatttgtac gacctgacca aagaccagct gttcaatcac
     4321 ttcagcgggg atctcgctgc gtgccagcag ttcgcctacc accatcttcc ctaaatcaac
     4381 cgtgggaatg ccatgaaaag ccgtcgcctg gcgggcaaaa ggcgtacgta aaccgctaac
     4441 aatggcgata cgatcgccct ggcgggtaac cagtggtaaa acctgaccca taacactccc
     4501 ctgtaaaaaa ataaataaag tggtctgacc tgatcatagt cttaaccatt tttttacatt
     4561 tagccaagcg gagaaaaagg aaagtgggag ctatgacaca gtgaagacag aagaaagaaa
     4621 agaaaacgcc cctgccagca actgacaggg gcgtacgtac ccgagaggaa ttaacgcaga
     4681 cccagctgga agatcagcat ttcagcttcg caggcaaaag tgaaatcgat atccaggcgc
     4741 acaccgtcag actcttcagt gaaagtcggg gagattttgc acggttcgga tttcacgcta
     4801 cgggattttt cagtcagcgc cgccagcgtt tgttctgctt ctgcgcggtt tgcaaacacg
     4861 cggctgtaag acgcggtgca gtcggagttg tccataatgg tgccaacatc catacagcag
     4921 caaaccgggg tttcatcagc actacattta ctcatcgttg atttcctctg tatgtgcacc
     4981 caaggtgcca gataaacgtt gtggatattt tacgcttccg gaaagtgctg ctccagttgt
     5041 taattctgca aaatcggata agtgaccgaa atcacactta aaaatgatct aaaacaaaat
     5101 tcacccgaat ccgcgagtgc gcaacctcca aattttgcca gctggatcgc gtttcttaga
     5161 tcatatttga aaaaagatgg aaacatactt gcaacattct agctggtccg acctatactc
     5221 tcgccactgg tctgatttct aagatgtacc tcagacccta cacttcgcgc tcctgttaca
     5281 gcacgtaaca tagtttgtat aaaaataaat cattgaggtt atggtcatga gccagaaaac
     5341 cctgtttaca aagtctgctc tcgcagtcgc agtggcactt atctccaccc aggcctggtc
     5401 ggcaggcttt cagttaaacg aattttcttc ctctggcctg ggccgggctt attcagggga
     5461 aggcgcaatt gccgatgatg caggtaacgt tagccgtaac cccgcattga ttaccatgtt
     5521 tgaccgcccg acattttctg cgggtgcggt ttatattgac ccggatgtga atatcagcgg
     5581 aacgtctccg tctggtcgta gcctgaaagc cgataacatc gcgcctacgg catgggttcc
     5641 gaacatgcac tttgttgcac cgattaacga ccaatttggt tggggcgctt ctattacctc
     5701 taactatggt ctggcaacag agtttaatga tacttatgca ggcggctctg tcgggggtac
     5761 aactgacctt gaaaccatga acctgaactt aagcggtgcg tatcgcttaa ataatgcatg
     5821 gagctttggt cttggtttca acgccatcta cgctcgcgcg aaaattgaac gtttcgcagg
     5881 cgatctgggg cagttggttg ctggtcagat tatgcaatct cctgccgggc aaactcctca
     5941 agggcaagca ttggcagcta ccgccaacgg tatcgacagt aataccaaaa tcgctcatct
     6001 gaacggtaac cagtggggct ttggctggaa cgccggaatc ctgtatgaac tggataaaaa
     6061 taaccgctat gcactgacct accgttctga agtgaaaatt gacttcaaag gtaactacag
     6121 cagcgatctt aatcgtgcgt ttaataacta cggtttgcca attcctaccg cgacaggtgg
     6181 cgcaacgcaa tcgggttatc tgacgctgaa cctgcctgaa atgtgggaag tgtcaggtta
     6241 taaccgtgtt gatccacagt gggcgattca ctatagcctg gcttacacca gctggagtca
     6301 gttccagcag ctgaaagcga cctcaaccag tggcgacacg ctgttccaga aacatgaagg
     6361 ctttaaagat gcttaccgca tcgcgttggg taccacttat tactacgatg ataactggac
     6421 cttccgtacc ggtatcgcct ttgatgacag cccagttccg gctcagaatc gttctatctc
     6481 cattccggac caggaccgtt tctggctgag tgcaggtacg acttacgcat ttaataaaga
     6541 tgcttcagtc gacgttggtg tttcttatat gcacggtcag agcgtgaaaa ttaacgaagg
     6601 cccataccag ttcgagtctg aaggtaaagc ctggctgttc ggtactaact ttaactacgc
     6661 gttctgataa cgcgttcgcc tggataaagt cacctgctat gcaggtgact ttaactcccc
     6721 cacttcaccg aaagtagttt cctcattgct tgcctcctga gttttgcaaa cagcctgatg
     6781 gcagcttgcc ctttcaaatc agtaagtggt atccacagac aaacctgaag ggaaatgcat
     6841 attttcaagc gttctgctcg tccttctcaa agagtttact tttctgcatt tccaggatac
     6901 tcaacccccc tggctattgt gcgcttatac actcaaatta aagataggtt ctaaataaat
     6961 gagcgttttt tgatagtcta tttcattagg taatatatat ttgtaacaaa tcaatcaaca
     7021 tggaataaaa tcatgctacc atctatttca atcaacaata ccagcgcagc ttacccagaa
     7081 tccatcaatg aaaataacaa tgatgaaatt aatggattag tacaagagtt caaaaacctt
     7141 tttaatggta aggaaggaat aagcacctgt attaaacatc tacttgagct tataaaaaac
     7201 gccatacgag taaacgacga tccttataga tctaatatta ataattcctc agttacttat
     7261 attgatattg gctccaatga tacagaccat attactattg gtatcgacaa ccaagaacca
     7321 atagaattac ctgcgaacta taaagacaaa gaactcgtcc gtactatcat taatgacaac
     7381 atagttgaga agactcatga tatcaataac aaggaaatga tcttcagcgc attaaaagaa
     7441 atatatgatg gagatcctgg ttttatcttc gataagatat cacacaaact cagacatacg
     7501 gtaacggaat ttgatgagag cgggaaaagc gaaccaactg acttatttac ctggtacggt
     7561 aaagataaaa aaggcgactc tctcgctatt gtaattaaaa ataaaaacgg aaatgattac
     7621 ttatctctcg gttactacga tcaggacgac taccacattc aaagaggaat tcgtattaat
     7681 ggtgatagtc tcacccaata ttgtagtgaa aacgccagga gtgcttcagc gtggtttgaa
     7741 agcagtaaag ctatcatggc agaatcattt gcaactggtt ccgatcatca ggttgtaaac
     7801 gagctcaacg gggaaagact
//
LOCUS       DAZSNM010000186         7757 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZSNM010000186 DAZSNM010000000
VERSION     DAZSNM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.186"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..856)
                     /locus_tag="ACHW42_003361"
     CDS             complement(<1..856)
                     /locus_tag="ACHW42_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR8945042.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNV"
     gene            964..1242
                     /locus_tag="ACHW42_003362"
     CDS             964..1242
                     /locus_tag="ACHW42_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945043.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="ACHW42_003363"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="ACHW42_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR8945044.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="ACHW42_003364"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="ACHW42_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR8945045.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="ACHW42_003365"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="ACHW42_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR8945046.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="ACHW42_003366"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="ACHW42_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR8945047.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..4880
                     /locus_tag="ACHW42_003367"
                     /pseudo
     CDS             4234..4880
                     /locus_tag="ACHW42_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4922..5452)
                     /locus_tag="ACHW42_003368"
     CDS             complement(4922..5452)
                     /locus_tag="ACHW42_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR8945048.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5608..6174)
                     /gene="slp"
                     /locus_tag="ACHW42_003369"
     CDS             complement(5608..6174)
                     /gene="slp"
                     /locus_tag="ACHW42_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR8945049.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6421..7644)
                     /locus_tag="ACHW42_003370"
     CDS             complement(6421..7644)
                     /locus_tag="ACHW42_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945050.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
BASE COUNT         2243 a         1529 c         1623 g         2362 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggttac gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg
     4381 aaatatggtt ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt
     4441 gctgctcagg tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac
     4501 caaaatattg taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg
     4561 ttattggcag cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc
     4621 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
     4681 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
     4741 ctgatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
     4801 ttcatgccac aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga
     4861 aaggcgtttc aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa
     4921 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
     4981 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct
     5041 ccagaatatc ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
     5101 gcgaaagtga cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
     5161 aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt
     5221 taatattaaa gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga
     5281 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat
     5341 gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa
     5401 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
     5461 cctggtgcgg acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt
     5521 ataataataa tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg
     5581 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
     5641 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
     5701 atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
     5761 catattcact tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg
     5821 aatggtaccg aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct
     5881 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc
     5941 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
     6001 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
     6061 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
     6121 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
     6181 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
     6241 tgaggtgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag
     6301 ttcaattccg ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt
     6361 gttttaaaat tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata
     6421 ttatggtatt atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc
     6481 tatttccacc ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg
     6541 gtatgaaatc ttttcgggtt gaagtgtctc gtaatgaaat tctatgaatt catttttaga
     6601 cattgttgcc catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg
     6661 tttataacat atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt
     6721 cactaccttt gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa
     6781 agagttgtca ttaatagtta acaatgtaga aaacaaacca ttgatttgtt tgtcaagatg
     6841 ttgttcatat ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa
     6901 taaatttgcg tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt
     6961 taggactagc ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc
     7021 aagggcttcc atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa
     7081 gtacaagctt tgaatgtctc ttatatcagt tggtaagtca gctcgttgcc cagcaaaatt
     7141 tttattatcg gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag
     7201 aattttagtt atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac
     7261 agaatcctta tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg
     7321 ttgcgtaagt ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac
     7381 cacaactttt gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg
     7441 ttggagttgt tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt
     7501 ggaattaatg ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt
     7561 tttaacatta tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat
     7621 acctggggta aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt
     7681 ttttaaattg gataattaga acaggaatgg attttcttct acacacaata aatataatgc
     7741 gttatatctt cctgata
//
LOCUS       DAZSNM010000187         7744 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZSNM010000187 DAZSNM010000000
VERSION     DAZSNM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.187"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="ACHW42_003371"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="ACHW42_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFR8945051.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="ACHW42_003372"
     CDS             1102..2562
                     /locus_tag="ACHW42_003372"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR8945052.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="ACHW42_003373"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="ACHW42_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR8945053.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="rspR"
                     /locus_tag="ACHW42_003374"
                     /gene_synonym="ydfH"
     CDS             complement(2979..3665)
                     /gene="rspR"
                     /locus_tag="ACHW42_003374"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR8945054.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="ACHW42_003375"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="ACHW42_003375"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR8945055.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="ACHW42_003376"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="ACHW42_003376"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR8945056.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="ACHW42_003377"
     CDS             complement(6727..7245)
                     /locus_tag="ACHW42_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR8945057.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7521..>7744
                     /gene="ydeI"
                     /locus_tag="ACHW42_003378"
     CDS             7521..>7744
                     /gene="ydeI"
                     /locus_tag="ACHW42_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR8945058.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKG"
BASE COUNT         1940 a         1811 c         1957 g         2036 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcttggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc gccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa
     7441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
     7501 aacgcaatta aggagttagt atgaaatttc agtccattgt attagcaagt tttcttgtca
     7561 tgccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc
     7621 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt
     7681 tcgcaaaaaa catgcacgat ggcgcttcgg tttcactacg cggtaatttg atttctcaca
     7741 aagg
//
LOCUS       DAZSNM010000188         7714 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZSNM010000188 DAZSNM010000000
VERSION     DAZSNM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7714
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.188"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..200)
                     /locus_tag="ACHW42_003379"
     CDS             complement(<1..200)
                     /locus_tag="ACHW42_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="involved in methylation of ribosomal protein L3;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR8945059.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTAR"
     gene            366..917
                     /gene="smrB"
                     /locus_tag="ACHW42_003380"
     CDS             366..917
                     /gene="smrB"
                     /locus_tag="ACHW42_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR8945060.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(1021..1911)
                     /locus_tag="ACHW42_003381"
     CDS             complement(1021..1911)
                     /locus_tag="ACHW42_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR8945061.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(1898..2422)
                     /locus_tag="ACHW42_003382"
     CDS             complement(1898..2422)
                     /locus_tag="ACHW42_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8945062.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(2419..2889)
                     /locus_tag="ACHW42_003383"
     CDS             complement(2419..2889)
                     /locus_tag="ACHW42_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8945063.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(2886..3434)
                     /locus_tag="ACHW42_003384"
     CDS             complement(2886..3434)
                     /locus_tag="ACHW42_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8945064.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(3439..4161)
                     /locus_tag="ACHW42_003385"
                     /pseudo
     CDS             complement(3439..4161)
                     /locus_tag="ACHW42_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(4181..6821)
                     /gene="yfcU"
                     /locus_tag="ACHW42_003386"
                     /pseudo
     CDS             complement(4181..6821)
                     /gene="yfcU"
                     /locus_tag="ACHW42_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(6903..7466)
                     /locus_tag="ACHW42_003387"
     CDS             complement(6903..7466)
                     /locus_tag="ACHW42_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8945065.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT         2006 a         1914 c         1776 g         2018 t
ORIGIN      
        1 cgcgcggtgc gcatatcttc cgggatatcc agcggcaggt agagcgatgg taacaccagc
       61 tgtacggctt catcccacgg gttatcggtg ccgtgaccgt accagatatt tgccgcgctg
      121 aagcggctta ccgaccagcg caatatgtcc tgaatggttt gcagctcatt tactgcttca
      181 tcaacgaaaa ttttatccac gtattcctcc agggcatgat cgcaataatt tcggcggcta
      241 gtttgccatg aagatgacga taaatcagca ttcacgcgcg gtgagtgagg aaaaatacgt
      301 ttaaaacgat cgattgtgct acgagtcggg taaactgtag gaaaattaga aatagagaca
      361 gataaatgaa aaagaaaaca acacttagcg aggaggacca ggctctgttt cgccagttga
      421 tggcggggac tcgcaagatt aagcaggaca cgattgtcca ccgaccgcag cgtaaaaaaa
      481 tcagcgaagt gccggtgaaa cgcttgatcc aggagcaggc tgatgccagc cattatttct
      541 ccgatgagtt tcagccgtta ttaaataccg aaggtccggt gaaatatgtt cgcccggatg
      601 tcagccattt tgaggcgaag aaactgcgcc gcggcgatta ttcaccggaa ttgtttttgg
      661 atttacacgg tctgacgcag ctgcaggcca agcaggaact gggcgcgttg attgccgcct
      721 gtcgccgcga acatgtgttt tgtgcctgcg tgatgcatgg gcacgggaag catattttga
      781 agcaacaaac accgctgtgg ctggcacaac atccgcatgt gatggcattt caccaggcac
      841 cgaaagagta cggcggtgat gcggctttgt tggtgttgat tgaagtggaa gagtggttgc
      901 cgccggagtt gccctgaatg ctggcagcaa atgccggagc agcgtaaacg ccttatccgg
      961 cctacaaata tgtgataatt caataaattg caattcactt cgtaggcctg ataagacgca
     1021 tcagagcaat actacgaact tcagttcatt caatacatac tgaaaacgac acgtcgaatc
     1081 aatcggttgc ccactactgg tattcacatt caccacaata tcgtgtagta ctttatccgt
     1141 taccggatga cgagttggcg taaccgttac cgttgattta tcacaaccgt aagaggtaaa
     1201 ggttaacaat tctgataccg cgcggcagta tgctcctctg ccatcattat cagaaaaatt
     1261 actgccatca cagagtctgc cgccttccat caggtaggtc gaaagcgtgg tggtcactgt
     1321 tgaccccgtg gactgaattc gtaacgcctt attatggctg acataatttt cactccagga
     1381 gatatcgtat gaagcgaccg gtatcgttgg gcatattgtg ttatgcactg taggggccgt
     1441 atagcttcca aatccgggta aatctgtata ggaataaaag gatgcgatcc aagggcaacc
     1501 ataatcatca gatgcaacgg taattttaaa cgtcatatat gcacaacgaa aaattttcgt
     1561 atctatgcca gggcaatttg agttattaat tgtatcaaag tcagtcagta ccaaagttgg
     1621 agctgaaccc gacccagacc aggaaataac ccgcaacgta tcgcctttat acgcaaaaga
     1681 atcatatttc ccatatgcaa cctcctttgg cgttatcacc gtaacattga aaacactgct
     1741 cgctacagca ccggttacct tgtccatggc ataataaact ctcacactgt caaaactcgc
     1801 actcataagt ttatctccag cataactctt agatgcgccg acaacactca gaacgagcag
     1861 cattaggctc gcgaaaatta tggtccattt tttcatttca gaaatactca aaggttgccg
     1921 tcactgtggc actaaagtct ccggacgtta catctcgccc gctctttgcc tgtaaccagg
     1981 tattaaaatt taggctgtta tttccctgat tcagcgggag cgtgacgcct gttgtattgt
     2041 tgattggcac cgatgtgcct gccgcagttt caataccaat cccagcgccg gatgctgatg
     2101 acgaggagtc aaaggccaga aagccgggta gagcactatc ttctgttcct gtcaacgtta
     2161 ctttgacatt aaacaatgta gaactatgac aatctttcaa ttgaaacacc aatggcactc
     2221 gctctgactg cccaaggttc attaaatcac tggcggcaat ggcaggaaaa tgtacttctg
     2281 ccaactggct accattaatc actggcgtac acgatttact gattaaattg ccggtaaagt
     2341 gcaaattatc tcctgctatt gtggttccac tgcccagcaa catcaggcta ctaataaaaa
     2401 acaacgtacg ctttttcttc attggtaatc tacctgtaac gtggcccagg cctcaaagtc
     2461 cgcctctggc agaatgacac cactcttttt tactggcaca gccgttaaca ccggtaaatc
     2521 ggtttcattc accacaagtg gcgtattgat cgtaaaactc tgccctgcct gtttgagctg
     2581 aatgcctaaa ccagtgatat tgctgcttac tgcagcatta tcaaaatcgc ttggtgtgcc
     2641 actccagctc aaggtcattt ccattaatgg gtcgcgaacc gccgtatcgc aggtaatgga
     2701 atacggcacg gtttgctcat aattgctgcc attaatgtca tcaataagca catcctgaaa
     2761 agttatttca atggtttgag cgttgctaat cgtgcagtta ggcggctgca ctaaataacc
     2821 gtgaaaagag acgtttgaga gtgccatcga gcaaaagccc cacagcaaaa taagcgagat
     2881 ccgtttcatt ggtaatccac cacaattgtg gcactggcgt taaattcagc tgccgctaac
     2941 gttgtaccac tctgtttcac cggcacagct tccagaacgg gaactcccga ggaaaaatta
     3001 aatggcaacc atgagccaga ggttaagtcg agtattgtgt gatcactact tttctgaacc
     3061 cgaataccaa acccaggaat acccgtgctg ataactgttt caccgttaat cacgaccgtt
     3121 gtcgcctgca tctgtagacg caggtcatcc attgccagat tgttacaaac aagcgagtag
     3181 tcaatgggac gtttataatc aaccctattg atcttattaa tgaatacatt accaaactca
     3241 actgccgcac catttaccgt acaaggagga ggggcatcga caagcacccg taaattcata
     3301 cttactgact tactcgctgc aactgcaaag ccgctaccaa cacaacatat cagtaaccca
     3361 gttaccgccc agacagaaca agatcctttc atactggctc tcccggcctc agcctttgcg
     3421 gctctgctct tcatcgacct agcaactgtt accgttgcaa gtgaaaaaga gcggcatccg
     3481 agcgccatag tcgttgacat aggtcagtac cggcacgcta cccatcttca cattcagcgg
     3541 tgcagaggtt ttcggcgaca tcactattgg cgagaaaccg gctgccggat tgccattttt
     3601 ctgtgtgctg gcattactga aaatgacgta atacgacgtc gggttattga cggtaaattc
     3661 ctgaccgttg cgggttaaag taatcttata ttgctagggg tttttcatgc tgacgttctc
     3721 cagcgccttc ggtcgccaga acaacttaat acgcgtttgt agtgctattt gcagcgtgtt
     3781 ggctttgttt gattttggcg ggatctcacg cacgttgaag tagaacaggc tttcacgatc
     3841 ggcaggcaat ttgttgatat ccggcattcc ctgcactttt acctgcccgt tcatcattga
     3901 atcgatgcgt tgcaccggtg gcagaacagt taacggtgaa ctgatcttgt tacccttttc
     3961 atcttcgatc cagctttggg ccagataagg cagtttcgga tcgttattgc gcaacgtcac
     4021 gctgattgat ttgtcgcttt cattaaatat caaacgggta cgatctggcg ttacagaagc
     4081 ctgggcagac aaactggcag cgctaagcag taacgctagc gtcgtagcac ccaatttggc
     4141 agagcaaagt aaatcagaca tggatcattc cttaatcacg ttattggtta gctgaaacag
     4201 aaacaggggg attggtgggt gtcacctgtt gcgtctgctc ctgaatgaca ggctttatgt
     4261 catcaggaac aaccggcgcg acgttgcctt tatgctggca tggcagtaac aagccgttaa
     4321 acagatcggc aggcagcggg tccggcaggt tgatatcgca atgcgcaaca ccactccaga
     4381 acacactcat gtgttcgcca ggtttcactc cagccagata aacattgccg tcatcatcga
     4441 caaggcccac tgtctgctcg ttatcatttt ttacttctgc gccaaacgga ggatggctgc
     4501 cgtcttgtaa acgcagcacc gccatcgctt tttgaccact gatgacggca aatttgcggt
     4561 agccaatcgc cccttcagtt agcgtggctt gtaccaccga ctgggttgct tcggcgtttt
     4621 ccggcagttt gttgaggtcg atatacgcct gattgcggta atagttattg acgtcagaaa
     4681 cgacggcttt accaaacata ttggtataaa cagcagcacc gttaccttct accggaacat
     4741 cggcaacgcc atcggcatca atcaacaagc gtgtcccgcc catattctgg gtacggtgaa
     4801 gtgcgccacc gtgggcagta agcgttgcgc cgccctgtaa cgacaagccc gcagaggtgt
     4861 attgcccttc atggtagttc gcactgaggt caacctgcgc cagcgaacca tcatggctgt
     4921 aatagccgtc aacgctggtg tgtttgtcac tggtgccgac gttcaactga tagtgagtcg
     4981 cgtcatcgac acggctgaaa taaccgacct gactgctgtc cgtcccactg ccatagtacc
     5041 gttatagcta acggtactgt tgtcgcccca cggcatactg agcgaaatgt acatgccttt
     5101 gtcggcctgg ttgtcatact cgtaacggta gccagtcatc gagatgctga cattacgaat
     5161 actgcccata ttgaaatagt gcgagagcat gatgttgtag tttgtctgct cctcgcgatc
     5221 ccagtaggta tggcgggtat agttgagata aaccgaaaca cccgcatcgc ggaagttctg
     5281 gttataagtg gcggtgtaca tctctttgtc gttgcccgtg cgtaccattc cgctgtcact
     5341 ggcatccaga tactcgctca tggtcataaa gttctcttcc gagaagcgat atccagcaaa
     5401 agtaacgcgg ctgttgagct ggtcaaagtc tttggaataa ctcacacgga aggagttacc
     5461 gtccagcgaa cctttgccat aagcggtgtc tttatccagt ttggtgtgcg agtgagtaac
     5521 atcaaacgcg accgcgccga atgtagacaa atcgcgaccg acaccaagcg ccgcagactg
     5581 atagttttca tctcccagtg cgccgccata taacgaccag ccgttagcga tcccccagga
     5641 agcttcagca ccagaaaaaa attcaccctc gacatggtgt ccccactctt gcggacggcc
     5701 catcatgatc ttatagcgaa cctgacctgg acgagtgagg tatggcatcg aggcggtgct
     5761 gatgtcatat tcctgcacct ggccgttctg ttcttcaatg cgaatatgca acgtaccgga
     5821 gacagaatca ccaagatcct gaatacgaaa cggtccagcc ggaacctgcg tttcgtaaat
     5881 cacacgcccc atctggctga cggtcacttt tgctgtggtg tgtgccacgc cggaaatgtc
     5941 tggcgcgtag ccgcgcagat tgggaggcaa catttgatcg tcagtactga cactgccacc
     6001 aacatagtta aaaccatcaa aaatatcgga tctgaggtaa tcctcgccca gcgccagttt
     6061 ggcttttaat gatggtaacg cccgcctggc atagtagcga ctccactccc atttttttga
     6121 gtttcatcgc cgctgaattc atcgtcatca ttactgcgag tatgttgata gttagtctgc
     6181 cagtcagcac gcatacgcca cggccctagg ttaaccccaa ccgtcccgtt gccgctgatc
     6241 tcgttactat catcaccgcc attttcttcg tgccgggttt gtgcgttgat gctgtagtcc
     6301 gcgacgatcc cggagatgcc gtcatcccaa cgtgaaggcg gatccctgtc gggataagta
     6361 tattcgaggt aagcctgtgg cagtgaaatg actaatgcgg actggcttaa atcagcctta
     6421 atttccatgc cttccagttg accggatttc aggcatttag catcgtggct ccattgcaga
     6481 tttttcgcca cgtcttcttt taaaccaaac tgcgctacca gttccggtgt cagacaagca
     6541 tagcttttgc tcgcgtcatc ttcaccagca taccagtaaa tatcgtactc ttccgccaat
     6601 ggctgtttat ttagttgaac ctgtaaattg tattttccgg gctcaacata tccctggctg
     6661 gaaaaacgct tcatatcaat ttttgtgtcg ccttttaatt ccagaaaacg ggaatcaaac
     6721 tgaatgtcgt cttcagccca gacactgcta taactccctg acattgccag cgcaatgcac
     6781 cacggaagaa tccgtaatcg aaaaagagaa tggtcaggca tacacgtttc tatccgtttt
     6841 tattcttgtt cggtcaaaat acatttaaat cccccatagc agtgtcgcta tgggggtatg
     6901 ccttacaggt aagtaatctg gaaggtggta tttgcttcaa aattacccag atctggcgca
     6961 tcagctgcgc cgaccagcca ggcgttaaag ttcagtgtct gcttcgcttt gcctttgttg
     7021 gtagaagtgt ctttcactat tttctggtca atacccatgc cgcttttgta agaggtaccc
     7081 agagagtcac cgatcgccag gctaacattg ttaaatgccg caccggtatc ggtattgaaa
     7141 atggtgtaat aattgccgct atttgcagaa gaaacggtac cagtaaaggt agtggtcatt
     7201 gtttcctgag tatcaaatac gcaatcttgc agacgaatct ggaaactttt cggcgtggtg
     7261 gtgccgttat ttttcagttt ttcggcaccg atatcaccca tatcaacttc cagtttgtga
     7321 tcgtccggaa cgattgaaca cactgagtct tcaatggtgc cgtaaaaacg tgcagtaccg
     7381 ttgttacccg cagcgattgt tgccgtagag gtgaacacgc ccatcaccat tgccgcagca
     7441 atagctgttt taacaaactt actcattatt tgtccttaaa ctataaataa ttaaaaatat
     7501 aaacattgca atacattgat tcagtcaata gccaatgttt atacccagcc attgaatacg
     7561 gcatgcgacc caaaaagaac aggtcgtaga aaattgtatt cattggtgtg gggccgcata
     7621 gtgatgtttc cacacccctg atccaattta attttactaa agagtaaatg tagatttaat
     7681 taatatattg acagaagaaa ggttatctta agga
//
LOCUS       DAZSNM010000189         7693 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZSNM010000189 DAZSNM010000000
VERSION     DAZSNM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7693
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.189"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="ACHW42_003388"
     CDS             complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="ACHW42_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HFR8945066.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="ACHW42_003389"
     CDS             complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="ACHW42_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR8945067.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="ACHW42_003390"
     CDS             complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="ACHW42_003390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR8945068.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="ACHW42_003391"
                     /pseudo
     CDS             complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="ACHW42_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="ACHW42_003392"
     CDS             complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="ACHW42_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR8945069.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="ACHW42_003393"
     CDS             complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="ACHW42_003393"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR8945070.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6041..7180)
                     /gene="wza"
                     /locus_tag="ACHW42_003394"
     CDS             complement(6041..7180)
                     /gene="wza"
                     /locus_tag="ACHW42_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR8945071.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7489..7638
                     /locus_tag="ACHW42_003395"
     CDS             7489..7638
                     /locus_tag="ACHW42_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945072.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
BASE COUNT         1795 a         2013 c         1929 g         1956 t
ORIGIN      
        1 cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aacccggaat
       61 acagccagag aaatcaatgc taacggtaat tttttcttaa tcgcctcttt attcagatat
      121 tgaaatgccc actccagcaa ataaaacagg ataaaggtca taacccctga aaacgatccg
      181 gataatatta tccctgcgag aatcagggca tctgttttag gcgttttgat accaaactgt
      241 ttgatgctga gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt
      301 gctgtcgtgc gcttgccgcc gaatttaatg aaattcagca catagctgtt gctgtaaatc
      361 agatatttcg aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc
      421 tccaccgccg ccagcgccac caccagtccg accaccagat aaaagaaacg taatatctta
      481 cgatggttgt gtggcgaaat tgttttaaaa cgaatactcc acaccatgcc aataatgatc
      541 acaatataga caaacagcat agttgaagtg acgtatttac cggcatccaa cgactgacca
      601 aacaggtagt taaacgccgt gagtcccgcg ccaatcccta aggcaatcat caatttttta
      661 atgctgatgc gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg
      721 gggaaacttt cgccaagctg ggcgattttg acgttaacca gcaggtagat cagtggcagc
      781 agcaggtagc tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact
      841 gtaggcggcg cgactgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc
      901 gatttccggt ttgcttaact gtaccagttg cagcacctct tcttcactga cggttttacc
      961 accggatttt tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc
     1021 aatcgatagc gcctcacaca aaatcagcgg gtagttatcg acacgagaac tgaacaccag
     1081 cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt
     1141 aaccacgttg ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc
     1201 gcccagcgcc atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc
     1261 atgcgccacc actgcgattt tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc
     1321 cagaatcgct tcggttgcca tatcaatgcc attattgata atccggcaac gccctggccc
     1381 gtacagacta ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag
     1441 cgccagcatc tcgcggaata actggcgttt gcccgccacc agctggtgcg cgcgatcaat
     1501 cttcaccggc ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc
     1561 gcaaccgtcg gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac
     1621 cagggtgaca tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctgttaag
     1681 attcaaccag tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt
     1741 aatggtgcga tataactcat tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa
     1801 cgcaatgttc gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg
     1861 atggctgacg ctctctttgc cgcctttgcc gtagccgtag acaaaatgtg acgccagccc
     1921 ctgttgcagc gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag
     1981 tcgcacatta aattgcagaa tattcattta atcaccttca ctcgcgcttt ttctcccacc
     2041 accagcgcgt tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg
     2101 ttaccaagcg tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca
     2161 atgtgtggac atgccatgtt atcggcaccc cgattgccga tagtgacgcc gtggcgaatg
     2221 gtgaaatcat cccccgccac cacgttttta ttgatcacta cggcgtaacc gtgatggata
     2281 gtaaagcggc ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg
     2341 ataatgcgat acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg
     2401 cgccacaccg aacaaaaatg agcgacgcga taggcaagta ccatgcagca cgggcgtaaa
     2461 ctccagctgt tggcgcgcag atcttccagc atctttagcg cccccgaata ccatcagcca
     2521 gacgtttgcc gttacgcacc gacagcagcg tcaacaaggt gcgccaggtc atacgtttat
     2581 tgcggatctg gtagagggta aacagctgat attttttact ggcgcggtcg aatttgtctt
     2641 tgtgcttgcg gtaaaagtgg aagtatccgg agaatttttt cggtgacgag gtgatctgca
     2701 tttcgccgtg attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccgt
     2761 actccaccac catccgcagg aatatgtcgt aatcctgtgc tgctttcagt tcggtatcga
     2821 acaaacactc tttgaaacgc catgcccagg taaagacctg attgccaatg atattacgtt
     2881 tatagaacag gcggcgtgag tacggcgatt tgggatacag tggtaagctc gccggttggg
     2941 aataaacttc tccctagcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt
     3001 gctgtttatg ggcgaggaag acgctcaggc ggttgggcgt ccattcatca tcatcatcga
     3061 tcccggtgat gtattcccct tgcgccagca taatcgcctg gttacgcacc gcgcacgccc
     3121 cgctgttaat gtcgttgtgg atgtaagtga tacgcggatc gttgagggca gtgacgtact
     3181 gttgcagttg ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt
     3241 agtcctggcg caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggttg
     3301 gcatatagat tgagatcagc ggattgtctt tcataggttg ttctcccggc ttatgagcta
     3361 gtttgcttgc cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag
     3421 gcgtcgttat ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc
     3481 gctggcgcgg cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc
     3541 aaagcggctc agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat
     3601 taacgtggtt ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg
     3661 cgtatcaatc aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg
     3721 ttcgctcatt aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa
     3781 tttggcaata gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatctc
     3841 cgacagccca ttgacattat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc
     3901 gcagtcgatc aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca
     3961 gacaaaggtt ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc
     4021 ctgcatcatc gcgaagtgca gactggtacg cagactacgg atggcttcaa tcgccagatc
     4081 ggttggattc cccactgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt
     4141 tttgacgcta tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat
     4201 tccgtgttct tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag
     4261 cacacccacg atagagagca tcaggccaag gataatcgcg ccgaggataa tcagcccttt
     4321 cttcggtttt agcacgccag gctgggtgat tgccgggtca ataatgcgca catcgccgac
     4381 ggtgctggct tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg
     4441 ctgaccagac tcgacatcgc gggtcagacg gacaatttcc tgctggtttt tcggcatcgc
     4501 cgttacgcga ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag
     4561 cgtgcggtac gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt
     4621 cagttcgttc aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttcaag
     4681 cggcagatca acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc
     4741 gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc
     4801 cgccgatttg cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc
     4861 gcggatctgt tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc
     4921 gttctccgtt accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt
     4981 ggtgacggta aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc
     5041 ttcttttttc agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag
     5101 ggtgtagttt ttgtcatcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg
     5161 gcggttaaag gtagtcactt tcaccgtctc gttctgacgc cccatcaggc gatcccagcc
     5221 cgccccgaaa atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac
     5281 cgttttacca agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg
     5341 tttgttggct aatgccgagc cgatatcctg caccagcgaa ttgccgctgt tttgctcgat
     5401 ttgtaccagt gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca
     5461 gagggcaaat acgacggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac
     5521 caggcgacca atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt
     5581 ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt
     5641 tcaagtaatg tgtacaccgc tgcaaacgtt tcccggcttt tgtgatacgg atcggggatt
     5701 tcacattcgt tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc
     5761 tcgcataagc gttcgatatg gcgcttatcc atggtcaaaa tcaggtcata gttgcgacac
     5821 aggcggcggc tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg
     5881 acgctgatag cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc
     5941 actttcagtt ccgggtgata acgttgcagt aagcgttccg ccatcgggga acggcaaata
     6001 ttgccgacac agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc
     6061 tggctgtatc cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac
     6121 ggttccagcg ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga
     6181 attctgtcgc catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa
     6241 tcttgccgtt acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt
     6301 tggaggtggt catgtcgata ccttcggcat tggccagagc ttcagtcagg gtcatgccgc
     6361 taaagtccat tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat
     6421 catcattacg cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt
     6481 cgccgttttg catcagcgcc tgtaaagaaa tgcgttcttc acgaccattg tgtgttagca
     6541 ccacgttgcg ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa
     6601 tggtcagtgg tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat
     6661 aggccttttg tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg
     6721 tcgctaagcg cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa
     6781 ccttgccgat atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc
     6841 tggagctgcg gtactgacca gctggcgtgg tgagttccgg gtgatcccag acggtgacat
     6901 taagaacgtc ccccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca
     6961 tattggggcg cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg
     7021 gataaacatt caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt
     7081 tgcccatcgt cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg
     7141 acaccaataa tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc
     7201 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
     7261 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
     7321 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
     7381 tagctaccaa tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga
     7441 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
     7501 tattggctcg ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
     7561 acaattcggc aattggctat ttttaagaat tatattaagt atcattcaat atggttttta
     7621 ggagtttctt taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat
     7681 aatgttttat tat
//
LOCUS       DAZSNM010000190         7662 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZSNM010000190 DAZSNM010000000
VERSION     DAZSNM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.190"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1159)
                     /gene="glpD"
                     /locus_tag="ACHW42_003396"
     CDS             complement(<1..1159)
                     /gene="glpD"
                     /locus_tag="ACHW42_003396"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR8945073.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVL"
     gene            1349..1675
                     /gene="glpE"
                     /locus_tag="ACHW42_003397"
     CDS             1349..1675
                     /gene="glpE"
                     /locus_tag="ACHW42_003397"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR8945074.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            1720..2550
                     /gene="glpG"
                     /locus_tag="ACHW42_003398"
     CDS             1720..2550
                     /gene="glpG"
                     /locus_tag="ACHW42_003398"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR8945075.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            2567..3325
                     /locus_tag="ACHW42_003399"
     CDS             2567..3325
                     /locus_tag="ACHW42_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR8945076.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(3307..4905)
                     /gene="rtcR"
                     /locus_tag="ACHW42_003400"
     CDS             complement(3307..4905)
                     /gene="rtcR"
                     /locus_tag="ACHW42_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFR8945077.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            5086..6312
                     /gene="rtcB"
                     /locus_tag="ACHW42_003401"
     CDS             5086..6312
                     /gene="rtcB"
                     /locus_tag="ACHW42_003401"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR8945078.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            6316..7332
                     /gene="rtcA"
                     /locus_tag="ACHW42_003402"
     CDS             6316..7332
                     /gene="rtcA"
                     /locus_tag="ACHW42_003402"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR8945079.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(7375..>7662)
                     /locus_tag="ACHW42_003403"
     CDS             complement(7375..>7662)
                     /locus_tag="ACHW42_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR8945080.1"
                     /translation="RHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSG
                     YSNEQIAGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
BASE COUNT         1760 a         1951 c         2161 g         1790 t
ORIGIN      
        1 gcagcacgct ctctttcgtc cacgccgggc caataccctg ataatacggc gtcagttttt
       61 ccagcgcatg ttctgccagt tttcggtagg tggtcagctt accgccgaat accgacagca
      121 gcggtgcttt gccgttttca tcatggatat caagggtgta atcacgggta atggcttgcg
      181 gcgagtcgga ctcatcatca cacagcggac gcacaccgga gtaggtccag acgatatcgt
      241 cacggcttag ctgcttttta aagtgcgcgt tatacacttt cagcaggtaa ttgatttcac
      301 tctcttcaat cttcactgct ttcggatcac ctttgtattc cacatcggtg gtgccgatga
      361 tggaaaactc gtccatccac gggatcacga acacaatgcg tttatcttcg ttttgcagaa
      421 tgtaggcctg cttttgggta tgcacgcgcg gcaccacaat atggctgcct ttgatcaggc
      481 gaatgccata aggcgaaggc agatgcatcc cctcgtcgaa gaactgtttc acccacgggc
      541 cggtggcgtt aaccaggccg cgcgcttgcc agctatattt tttgccggta tcgatatctt
      601 ccgcttccac aatccacagg ccgttttcgc ggcgagcaga ggtggcgcga gtccgagtaa
      661 gcacttcgcc gcctttacgc accaccatct gggcgttggc gagtaccaga cgggcgtcgt
      721 ctacccaaca gtcagaatat tcgaatccgc gcttaatttc cggttttaac actgaatttg
      781 cgccaaaacg caaaccagtt gatcccggca ggctggtgcg tttacccaga tgatcgtaca
      841 taaacagacc aatgcgaatc atccacgccg ggcgcagatg cggacgatgt ggcaggcgaa
      901 aacgcatcgg gaaggcgata tgcggagcca ttttcagcag cacttcacgt tcagccagcg
      961 cctcgctgac caggcggaat tcatagtgct caaggtagcg caggccaccg tgaatgagtt
     1021 ttgaactggc ggaagaggtc gcgcaagcga gatcctgcgc ctccagcatc agcacggata
     1081 aaccgcgtcc agcggcgtct gccgcgatac cagcaccatt gatgccgcca cctatcacaa
     1141 tcagatcttt ggtttccatg ctgccctcat tcactttcgt taaagctcat aaatgttcgt
     1201 tatcgaacat attagcaaag aatcgcgctt tgggtaacat tgaaaaaaca ttttagagtg
     1261 atatgtataa cattatggcg tttatctgcc gcttcgacgt aaactgtgcg gtaaatttgc
     1321 ccacttgttt gtaaagaaag agagacgcat ggatcagttc gaatgtatta acgttgccga
     1381 cgcgcaccag aagttgcagg aaaaagaggc ggtgctggtc gatattcgcg atccacagag
     1441 tttcgctatg gggcatgcgg tgcaggcttt ccatttaacc aacgacacgc tgggcgcgtt
     1501 tatgcgtgat aacgactttg acactccggt gatggtgatg tgttatcacg gcaatagcag
     1561 caaaggggcg gcgcagtatc tgctgcaaca gggctacgat gtggtctata gcattgacgg
     1621 cggctttgaa gcctggcagc gacagtttcc cgcagaggtg gcgtacggcg cgtaacgctt
     1681 tatactgtcc ccttttgtgt ggaataagcg acagcaacga tgttgatgat tacctctttt
     1741 gctaaccccc gcgtggcgca ggcgtttgtt gattacatgg cgacgcaggg tgttatcctc
     1801 acgattcaac aacataacca aagcgatgtc tggctggcgg atgagtccca ggccgagcgc
     1861 gtgcgggcgg agctggcgcg ttttctcgaa aacccggcag atccgcgtta tctggcggcc
     1921 agctggctgg caggccatac cggcagtggc ctgcattatc gccgttatcc tttctttgct
     1981 gccttgcgtg aacgcgcagg tccggtgacc tgggtgatga tgatcgcctg cgtggtggtg
     2041 tttattgcca tgcaaattct cggcgatcag gaagtgatgt tatggctggc ctggccattc
     2101 gatccaacac tgaaatttga gttctggcgt tacttcaccc acgcgttaat gcacttctcg
     2161 ctgatgcata tcctctttaa cctgctttgg tggtggtatc tcggcggcgc ggtggaaaaa
     2221 cgcctcggta gcggtaagct aattgtcatt acgcttatca gcgccctgtt aagcggctat
     2281 atgcagcaaa aattcagcgg gccgtggttt ggcgggcttt ctggcgtggt gtatgcgctg
     2341 atgggctacg tctggctacg tggcgaacgc gatccgcaaa gtggcattta cctgcaacgt
     2401 gggttaatta tctttgcgct gatctggatt gtcgccggat ggtttgattt gtttgggatg
     2461 tcgatggcga acggagcaca catcgccgga ttagccgtgg gtttagcgat ggcttttgtt
     2521 gattcgctca atgcgcgaaa acgaaaataa ttccagggat ttataaatga aacaaacaca
     2581 acgtcacaac ggtattatcg aactggttaa acagcagggt tatgtcagta ccgaggaact
     2641 ggtggagcac ttctccgtca gcccgcagac tattcgccgc gacctcaacg agctggcaga
     2701 gcaaaacctg atcctgcgcc atcatggcgg cgcggcgctg ccttccagtt cggttaacac
     2761 gccgtggcac gatcgtaaag ccacccagac cgaagaaaaa gagcgcatcg cccgcaaagt
     2821 ggcggagcaa atccccaatg gctcgacgct gtttatcgat atcggcacca cgccggaagc
     2881 ggtggcgcac gcgctgctca atcacagcaa tttgcgcatt gtcaccaaca atctcaacgt
     2941 tgctaacacg ttgatggtaa aagaagattt ccgcattatt ctcgccggtg gcgaattacg
     3001 tagccgcgat ggcgggatca ttggcgaagc gacgctcgat tttatctccc agttccgcct
     3061 tgatttcggc attctgggga tcagcggcat cgacagcgac ggctcgctgc tggagttcga
     3121 ttaccacgaa gttcgtacca agcgcgccat tattgagaac tcgcgccacg ttatgctggt
     3181 ggttgaccat tcaaaatttg gccgtaatgc gatggtcaat atggggagca tcagcatggt
     3241 tgatgccgtc tacaccgacg ccccgccgcc agtaagcgtg atgcaggtgc tgacggacca
     3301 ccatattcaa ctggagctgt gctgatcctg cacggcttcc cacgtcagac caaaacgcgc
     3361 caggtatttg cgtagccgat ccgcgtcatt gacgctggct ttgccctggc gcgaaacatc
     3421 aaaaagctgt cgtccggcag cagaaagcga ctttgcctgg cggcagatag cgataacgtg
     3481 ttccagttgc atacggtcga agagatcgat gttttctgcc tctgcgccca gcaacgccgt
     3541 aagcgcggag gggcggctct cctgccagtt atagcgcaga cggtttatct catcttccac
     3601 cacgtccaga gtgatgcgtc cgctagtggc aaaggtggcc atccgcgtga cgctggcaga
     3661 aagttcgcga aagttaccgc gccatgttgc ctggggagat gttgcaaagg ccagccaggc
     3721 acgccgcgct tcggtgttaa aacgcacgct gtcaccagtg agtgtggcgt ggcgctccac
     3781 ttcataatcc aggttcggtt caatatcttc ctggcgctgg cgtagacccg gcagggtgaa
     3841 ggtccagaga ttgatccgcg cgtacagatc ttcgcggaat ttgccttcgg caaccagctg
     3901 gcgcaaatcg cgcaccgttc cggcgataag ctgaaaatca ctgctcacct ggcgatcgct
     3961 gccaaacggg taaaaggttt tctcttcaat agctttcagc agcattgcct gttcgtctgc
     4021 gcccagttcg ccaatctcat caagaaacaa cattccgccg ttggcgctgc gtaataatcc
     4081 ttcacgagat tcccgcgccc cggtaaacgc gcctttcaca tgaccaaaca gcgtcgacat
     4141 cgcggtatcg ccgcgcaggg tggcgcagtt cacttccaca aacgcgccgc taaactgatg
     4201 ccgtgcctgt tttaactcga agatgcgtcg cgccagaaat gacttacctg cgccggtcgg
     4261 gccgttaagc agaatcggcg cgcgggattt aatcgccact ttttcgatct gctcaatcat
     4321 gcggttgaag tgggggttac gcgtggcaat accggatttg aggaaatcga gcgtttgctg
     4381 ccgttcctcg gcaaagcggc tggcgatggc gttataacgg cttaaatcga gatcgataat
     4441 cgtcacttca cctgggccgc gcggctgttc ttttttgcgc ggagttgaag attgtatcaa
     4501 ccgggcgggt agataacgcg cttccgccag cagaaaccag caaatctgcg cgacgtgggt
     4561 gccggtggtg atgtgaatta aatagtcttc tttttctggc tgaaactcgt aaccacgggc
     4621 gaaatcatgc aggcaggcgt aaacctcttc gaaatcccac gggttatgca gttcaatctc
     4681 aacgcccacc acttctgttt ctggcgaaac gctggcgata tcgcgtttaa gcgtttcaaa
     4741 tagcgagcgc gagcgggcat cgtgcaataa ttccagtcga tcgatgacca acgattcttg
     4801 ctggtatata cagagtgacg gacgccattt tgaccagcgc tgactgccgc gtccggcata
     4861 atccagcacg gtaccgacaa agccaaaagc cactgtttta cgcatcttag atatccttat
     4921 aaaaggcgat atttcattat cggtaaatcg gctttgcggg aaatctccgt ttacggatat
     4981 aaagatataa tctttttaga aacaataaat taatctgctt atttaatttt ctggcacgac
     5041 ggttgcaatt atcaggaccg caaacaacga aaagagaaaa acaaaatgaa ttacgaatta
     5101 ctgaccactg aaaatgcccc ggtaaaaatg tggaccaaag gcgtgccggt agaagccgat
     5161 gcgcgtcagc aacttattaa tacggcgaag atgccgttta ttttcaaaca tattgcggta
     5221 atgcctgatg tacacctggg taaaggttcc accattggta gcgtgatccc gaccaaaggg
     5281 gcgattattc cggcggcggt gggcgtggat attggctgtg gaatgaacgc gctgcgtacc
     5341 gcgttaacgg cggcagacct gcctgaaaac ctggcggagc tgcgtcaagc gattgaaacg
     5401 gccgtaccac acgggcgtac taccgggcgt tgtaaacgtg ataaaggcgc atgggaaaac
     5461 ccgcctttta acgtcgatgc gaaatgggct gagctggagg ccggttatca gtggttaacg
     5521 caaaaatatc cgcgtttcct taacactaac aactataaac acctgggaac gctgggaacg
     5581 ggtaaccact ttattgaaat ctgccttgat gagtcggagc aggtgtggat tatgctgcac
     5641 tccggttcac gcggaattgg taacgccatc gggacttact ttatcgatct ggcgcaaaaa
     5701 gagatgcagg accaacttga gacgttgccg tcgcgtgatc tggcgtactt tatggaaggt
     5761 acggaatact ttgatgatta cctaagagcc gtggcctggg cgcagctttt cgccagcctt
     5821 aaccgcgatg cgatgatgga aaatgtggta acggcgttgc agagcattac gcagaaaacg
     5881 gtcagacagc cacaaacgct ggcgatggaa gagatcaact gtcaccacaa ctatgtgcaa
     5941 aaagaacagc tctttggtga agagatctac gtgacgcgta aaggtgcggt gtctgcgcgt
     6001 gctggtcaat atggaattat tcccggttcg atgggagcaa aaagctttat cgtccgtggg
     6061 ctgggaaatg aagagtcgtt ctgttcgtgc agccacggtg ccgggcgggt aatgagccga
     6121 actaaagcga aaaaactgtt cagcgtggaa gaccaaattc gtgccaccgc gcatgtggaa
     6181 tgccgtaaag atgccgaagt gatcgacgaa atcccgatgg cgtataaaga tattgatgcg
     6241 gtgatggcgg cacaaagcga tctggtggaa gttatctaca ccctgcgtca ggtggtgtgc
     6301 gtaaaaggat aaatgatgaa aaggatgatt gcgctggatg gcgcacaggg cgaaggcggc
     6361 gggcagatcc tgcgctcggc gctgagcctg tcgatgataa ccggccagcc atttaccatc
     6421 actggcattc gagccggacg ggcgaaaccg gggctgctgc gccagcatct gaccgcggta
     6481 aaagcggctg cggaaatttg tagggcaacg gtggaaggcg cggagctggg atcgcagcgt
     6541 ctggtcttcc ggcctggcac cgtgcgcggc ggcgattacc gctttgctat cggtagcgcc
     6601 ggaagttgta cgctggtgct gcaaacggtg ctgcccgcgc tgtggtttgc cgatggacct
     6661 tcgcgtgttg aagtgagcgg cggcaccgat aacccgtcgg cgccgcctgc ggattttatc
     6721 cgccgggtgc tggagccgct gctggcgaaa ataggaattc atcagcaaac cacgctgtta
     6781 cgtcacggtt tttatcctgc cggaggcggt gtggtggcaa cggaagtctc gcccgtggca
     6841 tcgtttagca ccttgcaact tggcgagcgc gggaacattg tgcagatgcg tggagaagtt
     6901 ctattagctg gtgtgccgcg ccatgttgct gagcgtgaaa tcgctacact ggcggggatt
     6961 ttttccctgc atgaacagaa tattcataac ctgccgcgcg accaggggcc gggtaatacc
     7021 gtctcgctgg aagtcgaaag tgaaaatatc actgaacgct ttttcgtcgt cggtgaaaag
     7081 cgcgtcagtg ccgaggtggt agccgcgcag ttagtgaaag aggtgaaacg ctacctggca
     7141 agcccagcgg cggtggggga atatctcgcc gaccaactgg tactgccgat ggcgctggcg
     7201 ggcgcgggag aatttacggt cgcccatcct tcatgccatc tgctgaccaa tatcgcggtg
     7261 gtggagcgtt tcttgccggt gcggtttggt ttgatagaaa cagatggcgt aacgcgggtg
     7321 agtattgaat gatgtaaaga tgtaaaccgg atccaatgca ttatccggct aaactcacac
     7381 cccataaccc atcatcttca gcagtttctg cgcgtgctgc acggcagcct gacggtgggc
     7441 gacgccgagt ttttggtaca gattgcggat atgcgtcttg atggtggttg ccgccacttc
     7501 cagttcgccc gcaatttgtt cattgctgta accggagtag atcagcccca ggacctgcca
     7561 ttcacgttgg gtcaacgggc tggtgcggat tagttctggc acttccggat gatttagcag
     7621 acgttcaacg aaattctcat cgaaatgggc gaatttatgc cg
//
LOCUS       DAZSNM010000191         7641 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZSNM010000191 DAZSNM010000000
VERSION     DAZSNM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.191"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..85
                     /locus_tag="ACHW42_003404"
     CDS             <1..85
                     /locus_tag="ACHW42_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8945081.1"
                     /translation="RKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            103..1262
                     /gene="rbsA"
                     /locus_tag="ACHW42_003405"
                     /pseudo
     CDS             103..1262
                     /gene="rbsA"
                     /locus_tag="ACHW42_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1267..2232
                     /gene="rbsC"
                     /locus_tag="ACHW42_003406"
     CDS             1267..2232
                     /gene="rbsC"
                     /locus_tag="ACHW42_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR8945082.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2257..3147
                     /gene="rbsB"
                     /locus_tag="ACHW42_003407"
                     /pseudo
     CDS             2257..3147
                     /gene="rbsB"
                     /locus_tag="ACHW42_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3273..4202
                     /gene="rbsK"
                     /locus_tag="ACHW42_003408"
     CDS             3273..4202
                     /gene="rbsK"
                     /locus_tag="ACHW42_003408"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR8945083.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4206..5198
                     /gene="rbsR"
                     /locus_tag="ACHW42_003409"
     CDS             4206..5198
                     /gene="rbsR"
                     /locus_tag="ACHW42_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR8945084.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5164..6591)
                     /gene="mdtD"
                     /locus_tag="ACHW42_003410"
     CDS             complement(5164..6591)
                     /gene="mdtD"
                     /locus_tag="ACHW42_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR8945085.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6614..7306)
                     /locus_tag="ACHW42_003411"
     CDS             complement(6614..7306)
                     /locus_tag="ACHW42_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR8945086.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1949 a         1849 c         2011 g         1832 t
ORIGIN      
        1 acggaagtca ctgtcgttct caaaatcggt ggagctgcat gacaaggtca tcgggcatta
       61 tctgaacata aaacactatc agtaagttgg agtcattacc ggaaaaccat gtatgtcgaa
      121 gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagtt agtgggcgat
      181 ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt tgagtcgaaa
      241 gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga atccctgttc
      301 cgcgtcatcc gcgagctgaa atcgcaaggc cactgtattg tctatatctc ccaccgcatg
      361 aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgatgggca atttattgct
      421 gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt gggccgcaag
      481 ctggaagatc aatatccaca cctggacaaa gcgccaggag atatccgcct gaaagtcgat
      541 aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg cgaaattctt
      601 ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct ctacggcgca
      661 ctgccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac ccgttcaccg
      721 caggatggcc tggcaaacgg cattgtgtat atctccgaag accgtaaacg tgacggttta
      781 gtgctgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta cttcagccgc
      841 gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt cattcgtctg
      901 tttaatgtga aaacgccgtc gatggaacag gcaattggtc tgctttccgg tggcaatcag
      961 caaaaagtgg cgattgcccg cggtctgatg acacccccaa agtattgatc cttgatgagc
     1021 ctacccgtgg cgtagatgtc ggcgcgaaaa aagagatcta tcaactgatt aaccagttca
     1081 aagccgatgg cttgagtatc attctggtgt catcggagat gccagaagta ttaggcatga
     1141 gcgatcgcat catcgtcatg catgaagggc atctcagcgg ggaatttact cgtgagcagg
     1201 ccacccagga agtgttaatg gctgtcgctg tgggcaagct taatcgcgtg aatcaggagt
     1261 aaaaaaatga taacccagac tgtctctggt cgccgttatt tcacgaaagc gtggctgatg
     1321 gagcagaaat cgcttatcgc tctgctggtg ctgatcgcga ttgtctcgac gttaagcccg
     1381 aactttttca ccatcaataa cttattcaat attctccagc aaacttcggt gaacgccatt
     1441 atggcggtcg ggatgacgct ggtgatcctg acgtcgggca tagacttgtc ggtaggttct
     1501 ctgttggcgc taaccggcgc agttgctgca tctatcgtcg gcattgaagt caatgcgctg
     1561 gtggctgtcg ctgctgctct cacgttaggt gccgcaattg gtgcggtaac cggggtgatt
     1621 gtagcgaaag gtcgcgtcca ggcgtttatc gctacgctgg ttatgatgct tttactgcgc
     1681 ggcgtgacca tggtttatac caacggtagc ccagtgaata ccggctttac tgagaacgcc
     1741 gatctgtttg gctggtttgg tattggtcgt ccgctgggcg taccgacgcc agtctggatc
     1801 atggggattg tcttcctcgc ggcctggtac atgctgcatc acacgcgtct ggggcgttac
     1861 atctacgcgc tgggcggcaa cgaagcggca acgcgtcttt ctggtatcaa cgtcaataaa
     1921 atcaaaatca tcgtctattc tctttgtggt ctgctggcat cgctggccgg gatcattgaa
     1981 gtggcgcgtc tctcctctgc acaacccacg gcggggactg gctatgagct ggatgctatt
     2041 gctgcggtgg ttctgggcgg tacgagtctg gcgggcggaa aaggtcgcat tgttgggacg
     2101 ttgatcggcg cattaattct tggcttcctt aataatggat tgaatttgtt aggtgtttcc
     2161 tcctattacc agatgatcgt caaagcggtg gtgattttgc tggcggtgct ggtagacaac
     2221 aaaaagcagt aataacgact acaggacatc ttgaatatga acatgaaaaa actggctacc
     2281 ctggtttccg ctgttgcgct aagcgccacc gtcagtgcga atgcgatggc aaaagacacc
     2341 atcgcgctgg tggtctccac gcttaacaac ccgttctttg tatcgctgaa agatggcgcg
     2401 cagaaagagg cggataaact tggctataac ctggtggtgc tggactccca gaacaacccg
     2461 gcgaaagagc tggcgaacgt gcaggactta accgttcgcg gcaccaaaat tctgctgatt
     2521 aacccgaccg actccgacgc agtgggtaat gctgtgaaga tggctaacta ggcgaacatt
     2581 ccggttatca ctcttgaccg ccaggcaacg aaaggtgaag tggtgagcca cattgcatct
     2641 gataacgtac tgggcggcaa aatcgccggt gattacatcg cgaagaaagc gggtgaaggt
     2701 gccaaagtta tcgagctgca aggcattgcc ggtacatccg cagcccgtga acgtggcgaa
     2761 ggcttccagc tggccgttgc tgctcacaag tttaatgttc ttgccagcca gccagcagat
     2821 tttgatcgca ccaaaggttt gaacgtaatg cagaacctgt tgaccgctca tccggatgtt
     2881 caggctgtat tcgcgcagaa tgatgaaatg gcgctgggcg cgctgcgcgc actgcaaact
     2941 gccggtaaat cggatgtgat ggtcgtcgga tttgacggta caccggatgg cgaaaaagcg
     3001 gtgaatgatg gcaaactagc agcgactatc gctcagctac ccgatcagat tggcgcgaaa
     3061 ggcgttgaaa ccgcagataa agtgctgaaa ggcgagaaag ttcaggctaa gtatccggtt
     3121 gatctgaaac tggttgttaa gcagtagttt taatcaggtt gtatgacctg atggtgacat
     3181 aaatacgtca tcgacagatg aacgtgtaat ataaagaaaa gcagggcacg cgccacccta
     3241 acacggtggc gcattttatg gacatcccga atatgcaaaa cgcaggcagc ctcgttgttc
     3301 ttggcagcat taatgctgac cacattctta atcttcaatc ttttcctact ccaggcgaaa
     3361 ccgtaaccgg taaccactat caggttgcat ttggcggcaa aggcgcgaat caggctgtgg
     3421 ctgctgggcg tagcggtgcg aatatcgcgt ttattgcctg tactggcgat gacagcattg
     3481 gcgagagcgt tcgccagcag ctcgccactg ataacatcga tattactccg gtcagcgtga
     3541 tcaaaggcga atcaacaggt gtggcgctga tttttgttaa tggcgaaggt gagaatgtca
     3601 tcggtattca tgccggcgct aatgctgctc tttccccatc gctggtggaa gcgcaacgtg
     3661 agcgtattgc caacgcgtca gcgttattaa tgcagctgga atcaccactc gaaagtgtga
     3721 tggcagcggc gaaaatcgcc catcaaaata agactatcgt tgcgcttaac ccggctccgg
     3781 ctcgcgaact tcctgacgaa ctgctggcgc tggtggacat tattacgcca aacgaaacgg
     3841 aagcagaaaa gctcaccggt attcgtgttg aaaatgatga agatgcagcg aaggcggcgc
     3901 aggtactgca tgaaaagggt atccgtactg tactgattac tttaggaagt cgtggtgtat
     3961 gggctagcgt gaatggtgaa ggtcagcgcg ttcctggatt ccgggtgcag gctgtcgata
     4021 ccattgctgc cggagatacc tttaacggcg cgttaatcac ggcgttgctg gaagaaaaac
     4081 cattgccaga ggcgattcgg tttgcccatg ctgccgctgc gattgccgta acacgtaaag
     4141 gcgcacaacc ttccgtaccg tggcgtgaag agatcgacgc atttttagac aggcagaggt
     4201 gacgcttggc tacaatgaaa gatgttgccc gtttggcggg cgtttctacc tcaacagttt
     4261 ctcacgttat caataaagat cgcttcgtca gtgaagcgat taccgccaaa gttgaagcgg
     4321 cgattaaaga actcaattac gcgccatcag ctctggcgcg tagcctcaaa ctcaatcaaa
     4381 cacataccat tggcatgttg atcactgcca gtaccaatcc tttctattca gaactggtgc
     4441 gtggcgttga acgcagctgc ttcgaacgcg gttatagtct cgtcctttgc aataccgaag
     4501 gcgatgaaca gcggatgaat cgcaatctgg aaacgctgat gcaaaaacgc gttgatggct
     4561 tactgttact gtgtaccgaa acgcatcagc cttcgcgtga aatcatgcaa cgttatccga
     4621 cagtgcctac cgtgatgatg gactgggctc cgttcgatgg cgacagcgat cttattcagg
     4681 ataactcgtt gctgggcgga gacttagcaa cgcaatatct gatcgataaa ggtcataccc
     4741 gtatcgcctg tattaccggt ccgctggata aaactccggc gcgcctgcgg ttggaaggtt
     4801 atcgggcggc gatgaaacgt gcgggtctca acattcctga tggctatgaa gtcactggtg
     4861 attttgaatt taacggcggg tttgacgcca tgcgccaact gttatcacat ccgctgcgtc
     4921 ctcaggccgt ctttaccgga aatgacgcta tggctgttgg cgtttaccag gcgttatatc
     4981 aggcagagtt acaggttccg caggatatcg cggtgattgg ctatgacgat atcgaactgg
     5041 caagctttat gacgccacca ttaaccacta tccaccaacc gaaagatgaa ctgggggagc
     5101 tggcgattga tgtactcatc catcggataa cccagccgac ccttcagcaa caacgattac
     5161 aacttactcc gattctgatg gaacgcggtt cggcatagat ttacgctgtc ttttgatcaa
     5221 attattacca tcggttgttt tcagaagcat gaacattgct gctgaagcaa cagtaataat
     5281 gcccatcgtg ataaacgtat agtggaattg ttcgacagtc gttgtgcctt ccatcccttc
     5341 ataaatgcga aggacggccg cacttacagc aacgcctaaa ctaatcgaca gttgctgcgt
     5401 gaccgccaga acactgttac cgctgctggc gttgtcatcg gtcagatcgg caagtgtgat
     5461 ggtattcatc gcggtaaact gcgtcgacat agccatccct aatataaaca acggcaagat
     5521 cagcatccat atcgccattg ccggtgattg caaagagaac tgagcgatca ttagcccaat
     5581 aatcaccgtg atccccacta atgtatggcg atagcccaga cgacgtaaga cttgggtaac
     5641 catcgatttt gcaataatgg aacctaacgc tgtcggtgcc atcatacagc cggcaataaa
     5701 cgcctgataa ccaaatccta cctgtaacat caatggcata aggaacggta cacagccggt
     5761 ccccagacgg gttgcaatat tgcctacgat accgatcgag aaagtgcggg ttttaaataa
     5821 atctaatgaa attaatgggt ttggcgtacg tcgcgcatgg agaatataga gaagcagtaa
     5881 cccgatgctg gtgacaatta ccgtcaaggc aatccagctg gcgacaatct tttccccgaa
     5941 tagctctatt ccgcttgaaa agagaacaag gctgaggcca aacagcaaaa agccagtgat
     6001 atcgaatctg cgtcgtgcgg tggtgaaatt gggcatatgt ttgcgcgcgt aaagaaggcc
     6061 cgcaatacct atggggatat taattaaaaa tatccagtgc caggttgccc aggtgaccag
     6121 cacgccgcca agaacggggc ctaaaattgg ccccaccaga cccggcatgg cgacaaaatt
     6181 caataccgga agaagttcat tacgaggata agcacgcagt aaggccggcc gagcaacagg
     6241 catcatcatt gcgtcgccta tcccttgaat aacccggaag acaaccagct gtggtagcga
     6301 attagaaagt gcgcaggcca gagaacccaa cgtgaacaga ctcacggcaa gggtaaaaat
     6361 gcgacgcgta ccgaagcgat cggctagcca tccgcttacc ggaataagca tcgccaccgt
     6421 cagcgtataa ctgatgatgg ctgattgcat cgcgagagga gaacgattaa ggctatgagc
     6481 gattgcgggt aaggcggtat tcagaatagt ggcatcaagt gcctgcatga agaaggccat
     6541 cgccgcgatc cacggcaaac ccgacatact acgcttcttt ttatcgctca ttcaatgtcc
     6601 tgttatcggg ttatcactta tcaggtgagc gtagcagcgc ctgacaagct ttaaatgccg
     6661 cgtcgccatc gctttggata atcgcatcga caatcgcctg gtgcagatcc agctttatca
     6721 ctgtgtcgct ggtaattgac gtgaagtaag tgtgataaac cgaatggaat agcgaggcga
     6781 atgaggtcaa aaacggattg gcgctcattt catagatatg ctcatgccag gccatatcga
     6841 cttcgatcca gcgttcacgg cgaaagttct cttttaatgc cgccatttcg gccattaacg
     6901 tattgagatg cgccttctgt tccgcggtgc caaccgttgc tgccagtagg caggcttgcg
     6961 gctccagaca gatacgcata accagaaagt gatcgatgac ctgatgaaag ttctcttctg
     7021 ttatccacca agtaagcaat tcctgatcaa gaaaattcca gttcgattgt ggcatgaccc
     7081 gagtaccaat tcgcggtcgc ggtaaaacca tcccttttgc cgttaacgtt ttgaccgctt
     7141 cgcgtaccgc tgtacgactc actccaaatt gctcgcccag ctcaatctca ccgggcaaaa
     7201 tggtgccggg ttcatattca ccttttaaga tccgttgcgc cagcttctca gccagaacat
     7261 acgaaaggtt tttctgtgca gctaactgtt gtgcgcttaa aggcattact tatcttcctt
     7321 tttcttttta ttcctcctta gtatgccacc aggaagtgtg attacggttg caaaaacggc
     7381 aaattgcttg ttttatggca cattaacggg gtgttttgct gaaaaaatgc gcggtcagaa
     7441 aattatttta aatttcctct tgtcaggccg ggataactcc ctataatacg ccaccactga
     7501 cacggaacaa cggcaaatac gccgccgggt cagcggggtt ctcctgagaa ttccgacaga
     7561 aaaagcaaaa ataaatgatt gactctgtag cgggaaagcg tattatgcac accccccccg
     7621 cgccgctgag aaaaagcgaa g
//
LOCUS       DAZSNM010000192         7598 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZSNM010000192 DAZSNM010000000
VERSION     DAZSNM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.192"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            102..1157
                     /gene="phoE"
                     /locus_tag="ACHW42_003412"
     CDS             102..1157
                     /gene="phoE"
                     /locus_tag="ACHW42_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR8945087.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1196..1597)
                     /gene="crl"
                     /locus_tag="ACHW42_003413"
     CDS             complement(1196..1597)
                     /gene="crl"
                     /locus_tag="ACHW42_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR8945088.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1655..2899)
                     /gene="frsA"
                     /locus_tag="ACHW42_003414"
     CDS             complement(1655..2899)
                     /gene="frsA"
                     /locus_tag="ACHW42_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR8945089.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2991..3449)
                     /gene="gpt"
                     /locus_tag="ACHW42_003415"
     CDS             complement(2991..3449)
                     /gene="gpt"
                     /locus_tag="ACHW42_003415"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR8945090.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3711..5168
                     /gene="pepD"
                     /locus_tag="ACHW42_003416"
     CDS             3711..5168
                     /gene="pepD"
                     /locus_tag="ACHW42_003416"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR8945091.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5225..5839)
                     /gene="prfH"
                     /locus_tag="ACHW42_003417"
                     /pseudo
     CDS             complement(5225..5839)
                     /gene="prfH"
                     /locus_tag="ACHW42_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5901..6944)
                     /locus_tag="ACHW42_003418"
     CDS             complement(5901..6944)
                     /locus_tag="ACHW42_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR8945092.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            complement(7190..>7598)
                     /locus_tag="ACHW42_003419"
     CDS             complement(7190..>7598)
                     /locus_tag="ACHW42_003419"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8945093.1"
                     /translation="QLCAIFIRAVTMTVSQHYSLQQIAAWAQIDESRWKEKLAKSQVR
                     VAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIKSESELTVDASITAKP
                     FFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
BASE COUNT         1953 a         1928 c         1860 g         1857 t
ORIGIN      
        1 atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca acggggctct
       61 cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag agcactctgg
      121 cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa atatataata
      181 aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat atgagtgata
      241 acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt gaaactcaaa
      301 ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga aataaagcgg
      361 agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa tataaagatt
      421 tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa gcctggaccg
      481 atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt atgaccaaac
      541 gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcgttatc gatggcctga
      601 acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag caaaacggcg
      661 atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc attagtgggg
      721 cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc acaggcaagc
      781 gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat ctggcaactt
      841 tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat aagacacaga
      901 actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg ctgggttatg
      961 tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc aattatatcg
     1021 acgtcggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat tataaaatca
     1081 accaactgga tagcgataat aagctgaata ttaataatga tgatactgtc gcggttggga
     1141 tgacgtatca gttttaatga atattgccgg atgtgatgca tccggcacat ttcactcacg
     1201 ccgttaactt caccggttca tcacgaaaat cattcgccgg ttccagcttc agattcagcg
     1261 tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc agtcgttcaa
     1321 ccacttcagt atcttttacc ggaacactct tccagtcgcc tgctttatcg aacagaccaa
     1381 actggtagct gtaggtaaaa cgggattcct gcgcttccag ctccatccac cagccccaga
     1441 attcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa tcgaaaaaga
     1501 atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta aattttttga
     1561 tcaatctgct cttcgggtgt ccactcggta acgtcattgc tatctcctgt tgtgatgcaa
     1621 ctgttttacc aaattggcaa aatttagcaa atttttaaca caagcgtttt tcgatccagc
     1681 cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat gggatctcta
     1741 ataatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc gggctgaacg
     1801 gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgcccc agcaatcctt
     1861 gcacttttaa tgaatagcga ttcagctcca cgcgtaacgc ttcgtcggaa gcatcatgca
     1921 tccccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc tggcacttaa
     1981 aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc agacgcggcg
     2041 attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca aaggccgcga
     2101 cgcgagtgtg atccacccac ggtacgttag gcagcgcctt taagacgtgc tgatgcaaca
     2161 ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc atatcaatag
     2221 tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa tagtccgtct
     2281 gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg cctttcggca
     2341 tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc tgccgcatcg
     2401 tgcccggcag acgctgggcg gcctcttcat aggcgcggtt tgataaagcc tgcgcttgct
     2461 cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac gtagccgcat
     2521 gcagccagtg tcgaccactg agttgcggat cttcttccgt acaggctttt tgttgccaca
     2581 ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca atcacggtat
     2641 cgtataaatc atcgtcggta cgttcagcat cgctcatcac aatacgtgcc tggacgtcga
     2701 ggatttcacg tgggtcaata ccgcgccaga tccacatcag acggttaatc atgcgatacc
     2761 agtgagggat ggttttacca tcaagagccg actgcacagg cggttgtgcg ccgtgattaa
     2821 agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac agggtttcgc
     2881 tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac tattgtaacc
     2941 tgcctgaagt taaaaagaac aacgcccggc aatgccaggc gttgaaaaga ttagcgaccg
     3001 gagattggcg ggacgaatac gacgcccata tcccacggct gttcgatcca ggtatcttgc
     3061 gggatatcaa caacatagtc atcaaccagc ggacgaccag ccggttttgc gaagatggtg
     3121 acaaagtgcg cttttggata catttcacga atcgcaacag cagtaccacc ggtatccacc
     3181 aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgtttcag cactttaagc
     3241 tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg acgaataccc
     3301 agttcacgcg ccagtaacgc ccccggtacc agaccgccac ggcttacggc aataatgcct
     3361 ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat ctgcaacatg
     3421 tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt atctacggct
     3481 taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg cgcactaaaa
     3541 accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga cgcgggataa
     3601 agtggtattc tcaatacata tctcgcaagc ctgtcttgtg ttgacaacat tttctgctaa
     3661 ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac gtgtctgaac
     3721 tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt tctattcctc
     3781 acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca aaagagaaag
     3841 gtttccatgt cgaacgcgat caggtaggta atatcctgat tcgtcaacct gctaccgcag
     3901 gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg ccgcagaaaa
     3961 ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt gatggcgaat
     4021 gggttaaagc gcgcggcacc acgctgggtg cggataacgg cattggtatg gcctctgcac
     4081 tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg ctgaccatga
     4141 ccgaagaagc cggtatggac ggtgcgttcg gcttacagag caactggttg caggctgata
     4201 ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt gcggggggta
     4261 tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt tttgaaacct
     4321 tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc cacgttggcc
     4381 tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcagaa gaactggacc
     4441 tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt gaagcctttg
     4501 cgaccattgc tgtcgcagct gataaagtcg acgcactgaa atctctggtg aatacctatc
     4561 aggagatcct gaaaaacgag ctggcagaga aagagaaaaa tctggccttg ttgctggact
     4621 ctgtagcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt attcgtctgc
     4681 tgaacgccac cccgaacggt gtgatccgca attccgacgt ggcaaaaggt gtggtcgaaa
     4741 cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt cactgcctga
     4801 tccgttcact aatcgacagc ggtaaagact acgtggtgag catgctggat tcgctgggta
     4861 aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag ccggacgcta
     4921 attctccggt gatgcatctg gtacgtgaaa cctatcagcg cctgttcaac aagacgccga
     4981 acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg tatccggaaa
     5041 tggacatggt ttctatcggg ccaactatca ccggtccaca ctctccggat gagcaagttc
     5101 acatcgaaag cgtaggtcat tactggacac tgctgactga actgctgaaa gaaattccgg
     5161 cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc agtttcatcc
     5221 ttcattatcc ttcgataaaa gccatccctg taaacgttcg tcgcgggttg ccacgttcaa
     5281 tctggtgatg gaacattcgc cgctgtgatt tcagcaccgc gctattttcc tattgctgct
     5341 gatccagctt ccaggcaatc agcaatcgcg ccagccgttt gttagcatgc tggctgcgct
     5401 ctgactgaac cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc gccgagtcgg
     5461 ttttattgac atgttgaccg cccggccccg acgaacgcag cgtctcatac cggatcgcat
     5521 cggattgctc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag tttttacgcc
     5581 cgtgatgtgg gcgatacggg ctcgggcaaa tccactgaat agtgccacac cagctttcgc
     5641 ttaacgccca tgcgttgtcg ccatccagag aaatcagcgc cgaacgcagc gtgtcagagt
     5701 agcggcctgt ttctgtttcc agcaccgtta ccgcgacgtc ttgtcgggca gcttctttaa
     5761 ttagcctgtc cagcgccttt ttcactgcga gacaacattc ttccggcccc tgcgctgagg
     5821 agagttatag caagatcatc cttttttccc tccactgttt ttgagcgtta gcaccggacg
     5881 cagccgcgcg acaggaataa ttaatccggc ctgccctgac attgcaccac actttcagcc
     5941 gatttatagg cttgtggcgc ttcttcgaag atgagttgtt tatcgcgaca aattacccga
     6001 ctgccaagtt cagtacgcga gagttgcgtc gctgtgtatt tcgctgccag acgcccttta
     6061 cactcggtgc gcccccattt acgcccagcc ccatgcgcca gcgaatgcaa tgtttcctca
     6121 ctcctgacgg gctgaaccag cctggagtaa tcaccgcgtg aaccgggaat aaccaccaga
     6181 ccacagtcat caggcgtggc acctttacga tgtaagaccc cttgttgatc accgattcga
     6241 cacgcgctaa caaagttatg cgccacatcc agaaccggac ttccggtggc cttaacctgt
     6301 tgcattatgc gcaaagcgat caggtggcga ttaaaacgcg caaacgccag cgcgtcgtca
     6361 ctgccttcag gcaaaccatg atgcgaaaac gaggcaatat gccgctgtaa aatagactgc
     6421 cctagccccc tcgagccgct atgaaccagc agttgcagat gctgcgcatc cagacctgcc
     6481 agtgcaaaca gttcagcgtt gatgatttga tcaacttgtt gcagttcagc gaaatgattg
     6541 ccaccaccga tagagccaag cgagctgcgc cacggatgct gtgcaaatgc tgacggcagg
     6601 ttttcctcca gccagctttc ttcagcaacg tcatccaaag ctgataatcg cttttcaaac
     6661 ttatcggcgt tgtatttgcg agcgagaata tctgtttgcc atagcgccat gccacagccg
     6721 atatcattgc cgaccagtgc tgggtaaaaa cggcctgcgg agaagaacgc tgcgccaatc
     6781 gggtagccgc gtccggggtg taaatctggc atccccacta cgcgctgcat gttgggtaaa
     6841 tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc atcagatgcg
     6901 atggtaaata ccgcatcgga taagggacga atataattgc ccatatacca ttccgtaaat
     6961 agactcgtca tacttcaagt tgcatgtgct gcgacttgaa ttattgagag taacaattaa
     7021 aaaatcagga aatggcagac gaatccaggg caagaaaaaa agaattaatc ctggaaaggt
     7081 ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt ttgtggaata
     7141 agaaaacgcg cacatactag gcgggcgtta ttttcattgc aagctggatt taatgttgcg
     7201 gtttataacg catataaaaa ttaataaacc acgctccccg gcattcaacg cgctgctgct
     7261 taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctt gcgtccaccg
     7321 taagttcgga ttcagactta ataaaaggtt ttaacaaagc gctggcaacc ccacggcggg
     7381 tgtattcagg gtcaacaaat aacatatcga tatagtgttc aacgcaggta ataaagccga
     7441 ccagttttgc attaataact gcaacccgca cttgtgattt cgcgagtttc tccttccagc
     7501 gagattcgtc aatctgcgcc caggcggcaa tttgttgtag tgaataatgc tgactgacgg
     7561 tcatcgtaac cgctctaatg aaaatagcgc atagttgc
//
LOCUS       DAZSNM010000193         7581 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZSNM010000193 DAZSNM010000000
VERSION     DAZSNM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.193"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(592..2427)
                     /locus_tag="ACHW42_003420"
     CDS             complement(592..2427)
                     /locus_tag="ACHW42_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945094.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            2528..2815
                     /locus_tag="ACHW42_003421"
     CDS             2528..2815
                     /locus_tag="ACHW42_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8945095.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            2787..4316
                     /locus_tag="ACHW42_003422"
     CDS             2787..4316
                     /locus_tag="ACHW42_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFR8945096.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(4486..5703)
                     /locus_tag="ACHW42_003423"
     CDS             complement(4486..5703)
                     /locus_tag="ACHW42_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8945097.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(6070..7056)
                     /gene="ycdU"
                     /locus_tag="ACHW42_003424"
     CDS             complement(6070..7056)
                     /gene="ycdU"
                     /locus_tag="ACHW42_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFR8945098.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(7053..7581)
                     /locus_tag="ACHW42_003425"
                     /pseudo
     CDS             complement(7053..7581)
                     /locus_tag="ACHW42_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         2363 a         1758 c         1404 g         2056 t
ORIGIN      
        1 cccggaacta actaacctaa ccagacagat gatcactgta taaaaacttc agttaatact
       61 catttgtttt gtcgactcag tatataatga gtgaccagta atcaggataa tcacacttac
      121 ataacctgta aaaaagaaca agaagaataa gccactaaca taatgcaaaa tggcatctga
      181 atcgatcgca gtttggcatt cgaatcgatc atgatttggc ccaatccgtg atcgaataga
      241 gcggaatacg caacataatt caccagacaa gagcttacac cgaattacag attatgtatt
      301 aatgtcaaaa ctggcatatt ttacagatac aatgaacatg caacccatca atctaatagg
      361 ggcggtatgt acatctgccc atgaatggga tggcgctaag aatacaaaac aattagacat
      421 agatcacaaa aacacaccat tctaattgaa aaacaaaaca aacaaataaa tcatactatt
      481 atagaatttt ctcttgccag acatagataa tacccctgct aattttgcta atggttagca
      541 aaattagcag ggtatataaa gattattgac ccaaaacaca ctcagatgaa ttcacgacag
      601 ctcaggaggg ttattcctga tgtcatcttc acaaataatg gttaacgcat catatatcat
      661 tctgcgtctg ttcctgtttt cggccttatc gccatacaac atcatcacat ttttaatttc
      721 atcgaccggt gatttcacat agtgaactaa tacaccaagc ttctcaacac attgcagtaa
      781 agtatatttc ttactgcctg tgaaaagccc tatcatagtt tctttacgtt cgcgactgga
      841 taatactttg ttttccataa tgacttctgc cagcgtctta ccatcataaa catactcttt
      901 atctatcaga atatttatct tatccagcac cttctctacg tcatcaagca aacctgtaat
      961 atatgggggc gcaatatatg gtttcgtact gatcttttca agcatacaat tatacatctt
     1021 aataacacgc atcagggtaa aattgttcgc atcatactgt atttcaccct ccttatatac
     1081 aggatcaata agtggtattt tttcaataaa tgcatcctga taggctttta catggtcaag
     1141 ccaataaaac aacgagccat actttatatt ttttctattt actttaacct ccagtgctgt
     1201 ccgaagcaac gataatgccc ccctgacata tcctgaaggt aattcaccaa acaatccttt
     1261 tctttccatg tcatcataat gctgcagcac atcattaatt ttatttttcc ttatcagaca
     1321 aatgtacacc gcaagtgttt cgtagcagct ggtcacatga acagcctcat tctgcaactg
     1381 cccaaaatag cttataatct ttttcagata tgcgtcatca caaccattct tatcattaat
     1441 attaaataca agatctttca gacgggaaat atattcctga gatttatcat caagtgaaac
     1501 actataacga gacaatgttc cttctgcact gttttgaaac ttataaaaca tttccagtaa
     1561 tttttttgca taaggcgttg tatcaaagct ggtaaagtca taaagatatt gcgcagcgta
     1621 accatacaaa ccatgaatcc agagtccttc tctcaccaac actctgagat aatatgcaag
     1681 gctttgaagt atcttcgtct ttcgttgtat atcaagtaag acacttaatg aataaaatgc
     1741 attatagaat ttatcaatat tcctgagcac tttctccagt tcatccttat ccagcttctt
     1801 ctcccggtaa tcctttttat attcattttt gatagccatg atatattgtt gcatcaggct
     1861 cttgcttttc ccgttatcct tccgtatagt tcctgtatat ttccccagag catacagctt
     1921 tccattttca tgaaaatctt ccttgttgaa tcccagttca gccagatatt cccgaagata
     1981 acgtttaaca gcagcttcat cgctttctgt atcagcgtga tcaagacaag gacaatacga
     2041 tttctgtaaa aaattataaa tatgataata aaaagcagag tcattctctg gtctgtatgt
     2101 attatacata tctacaacaa gaaaaataac cccaaaagtg cgcagacaat agctgcgaag
     2161 gaattcaggt tttagtgtgg aatgcactgg ataagaattc atcatgtaat aaaaatgttc
     2221 atatcgggca tatatgatcc gccgttgctc ctgactgaac accccttcag catcaataat
     2281 acgactgatc tcattataac gttgatccca gaatttttga tgcatttcaa aattggtact
     2341 cttccgcttg tgtgccggat tctcgccttc cttaaacaac cccaaaacag tacaaatatc
     2401 atacaacata tttgtaaatg tgcgcatatg ctgtgctctc cggttggttt gcattttatc
     2461 gcagaggaca ttctgagctt aacgtatcaa actgacacaa cgacatgcaa tgcatatgga
     2521 gaacaatatg tcaaatcaaa ccggacagct ttacacgcct tcacgcatcc tgcgtatggc
     2581 tgaacttacc cgactgctgg gtatatcaag atctactatt tacgtgaaaa taaatccggc
     2641 ctcgaaatac cacgacccta cctttcccaa accaataagg cttggtagtg catccgtagg
     2701 ctggcgggca tctgccatcg atgagtggct gatgttacat acggcaccag cctggtctga
     2761 acctgaaaac aaactaaagg aacttaatga tgacgacaaa actatcactc cctgaacttg
     2821 acacaactaa actgcctgac aggtgccagg cattacttga tgaaatgcat gaagaaacag
     2881 gaatacgccg tgaaatactt ctgtctgtta tgctgacggt catggctgcc tcggtccagg
     2941 atacgcatga agttgaactt tccggagggc aacgtaccag ccttcagata tttatgtgtc
     3001 tttcatcagc atcaggcagt ggaaaaacct ctgcctgcgc aaaattaatc gctcctgttc
     3061 atgaaacaga agaagaacta catcaggcat atatcgacga taaaaaaaac tatgaccgaa
     3121 tgatggaaat gtggacaaca gataaaaaaa tcctggagcg gagatataaa aaggaaattg
     3181 aaagatcccc ggaaaatgct gctgctgcac gagcagcact tgaaaagtgt atagcaaata
     3241 aacccgaacc tcccgtacaa caggttctca tcgtgaatga cgcgacacca gaagggatag
     3301 ctttgaaact cagccagtca ccctctcttc tgttgctgtc tgatgaagga ggtactattc
     3361 tggataagcg tttcgaacgc aaatcggcac tgtacaacac attatggagt gggcaaccag
     3421 tcaacgttga acgggcatcc agacccgggt tccggctgaa tgatgtccgt ctcactatac
     3481 ttatactgac tcaaccggta atatttaata agttttttac tctcactggc gatcagatcc
     3541 ggggcaatgg ctttctggcc cggttgctat tttgcgaacc cggagcaatt aaaataatga
     3601 cgacagaacc gcatactgcc actcctgttg tgacgcaaca atgtgcaagc tgttttcgta
     3661 agaaaagttt tggcagtcag atcagagact ctctgagagc atccagagaa agacgtgcaa
     3721 aaagtaaaga gcgcatctgc atgacattat caaatgcagc atcccgcgca ctggagcgct
     3781 ttcacaaaga aaatatggat actgtcagac agaatcctca tatgggtaca tttgaagaca
     3841 ttattgtcag aaaaagagag caggttgtca ggatagcagc acttctggaa ctggagaaag
     3901 atccagactc gacagtaata acgctggaaa gtactaatag cgccatttat ctcattgaat
     3961 tttatttcaa gcatcttata tataaacttg aatcccttcg ggaaatatcc ccggctgaga
     4021 aacttgataa atggcttcag aaaagaatta tcacaacagc aggttacata ttccagaaaa
     4081 gttatatttt acaatatgcc ccatatgctc tcaggaaaaa atgtgttctg gatgaagcac
     4141 ttgacatact ggcggaacaa aggaaaataa ggattgatga taacctggtt gtttatatag
     4201 gaaacacgat tacaccatcc gaactggcaa aaaaactaaa tattcctgca ttcgatgccg
     4261 gagttttcat ttgtgatcat caaaacatac tgaagtatca ccgaaacaga ctatagatac
     4321 tatcggacaa aaaataaatt atcacaagtg tattattcgt tataataatg aataatcgtt
     4381 atctgagtgc aatgccaggt tttcattacg aactggcatt gcatttattt ttcaaaaaag
     4441 aaagcagtaa ccatttggct actctgagta tgccagtcca tgaaactaag cgaattcctt
     4501 agtgttttta gcaagttgag acatcacata atccgcccat gcctgcatca tcggtacacg
     4561 ctgctccagt agatcagtac gatgatatgc cgcctcaacc ttatttttca gcgtatgagc
     4621 gagcgccctt tccgccagat cccgcgaata cccctgttca ctacaccagt ccctgaatgt
     4681 tgagcgaaaa ccatgtgccg tggcaactcg ccccggaatg tcactgacgg ctttcttttt
     4741 acgcagaaaa cttgtcaaca ccatatcgga aaggatctgc tgctttctgg gtgaagggaa
     4801 caccagttca tcatgcaggc cacgtatatt ttccagaatg taaatagcct gccgggataa
     4861 aggaacacga tgctgtagcc tagctttcat tctttctgca ggtatagtcc atacccgctt
     4921 atgaaaatca atttcagccc agcgcattcc cctggcttcg cccgagcgag ttgctgtaag
     4981 tatcaccatt aataacagtg cgcgggtaac attataaggt tcatcactat acacactggt
     5041 cgccacaaaa agcggtaact gcctccaggg cattgcgggt tggtgttcat cacgtcctct
     5101 tgtctgctga ggaagcaaat ggtcaaccac atcaacagga tttgccacac aaaaaccgtg
     5161 cgcccatccc cactgcataa caacatgaat gcgctgttta acccggcttg ccgtttctga
     5221 caaggttaac cagactggac gcagtgtttc tgccacgtcc gcagccgtaa tcgaatccag
     5281 cgtttttgct cccagttgag gaaacgcgta attctcaagc gtcgataacc actgccttac
     5341 atgctttgga ttctcccatc caggagacag ttctgcatgt acacgcctgg ctgcatcggc
     5401 aaatgttggg ataacgactt tctcagattc agccttttta atctccagag gatcatcacc
     5461 tgcagcaagt tgctctcgca ttatccgggc agtacgtgca gcttcagcaa tactgacctc
     5521 tgggtaagtt cccaatccag cattacgtct tttttgtgtc accggactta cataacgaaa
     5581 aacccatttc ccccgcccct ttactgaaga aggatgaagg gtcagtccgg taattccccc
     5641 atggggcaat ggtttgtcat caggtttgat atgtcttgct ttcgtatccg tcaatactgc
     5701 catacgctaa ttcccgtcac tctggtatgc catccagtat gccattagtc cctcgcttca
     5761 ctaagaatac attagataac atcggacaac aaaaagtgta atacattgtt tataaagaaa
     5821 ttatataaca cccctggatt aagtcagatt tatttcaggc ggtccccctc accgccatat
     5881 ttagagaaga gcgcgtacga aagtacgggc ttttttttcg tatattgtac acaccgggtg
     5941 cccccgccca cgtctgttag ggcgagggga aattgtgcac atcaactgtc tggttactca
     6001 gtcaacaatc aaaatgcgta agatagtaca gaacatatat gcgttatctg gtcacttcct
     6061 gtaacttcct cacttattaa aatgccccca acggaaatac atgtcacatg ccatcataaa
     6121 atcgttatgc cagaggaatc gacaatggaa aaaatagtaa attaagcacg gtacatagca
     6181 gagtaaaaaa taggcttcaa accaaatccg ccattccaca agcgttgcca ataccgacac
     6241 cagcgtaatc cacatcattt caataacccg caatatgaat aaccgaagcc aaagcggctt
     6301 ggcaaaaaag gtcgacaacg tattttcact ggagaaaaac agatcgattt cctgcaaaaa
     6361 aaacctgccc agaaaagcgg gccgcttgga gaagcgcaga aaaaacagat taatcgccaa
     6421 cactacgcca caaagaatga gcaatgcaat tatcgattta aacgttaacc actgcccctc
     6481 tgtcacaaaa gaatcactgt tacgcagcgg gctgatagta agcaggttaa caaccgttga
     6541 gatgcacacc gaaagcagca catagcgagt cagactttta tattttactt ttttcgctac
     6601 tgaaatagcg acctgcttga cataactggt gcggatcatc cgaaacagca gaaagatgac
     6661 caacggccca agtaacgaaa tcaggaacag cgcatataac gagggtagaa gctggtatag
     6721 cccccaactc attgcactca acatgattaa cgacagaaat cctgccacca gaggttgtgt
     6781 aagaaataat tctattttgt ggttctgctt tttcttaaac cacgaaaaat cgatattctt
     6841 cgtgacgcat ttataagccc agtacgcctt gagatcaaaa ataaaattat acagcaccag
     6901 ggccagaatc ccactaacta caccaatatc ctctgatgga aaagtaaacc ctgcattttt
     6961 ccaataaaag cctatcagaa taagtccaat agcataaatg tatatcagat ttcgtttatc
     7021 ctgaaagcga ataaaagtta aataatcagg aatcatttga atacctttgc atcactaaat
     7081 gctttacgac aatgcataac tcgttgctga tccacgccag caaaatcatt taacatattc
     7141 cccaaatagg gcttattact tatttcaagg aaagctaccg gggttggcat attcagaata
     7201 tgtatcgaga gattcagtcc atttcaccgt atgagttaag tgtaatgaga gattttcttt
     7261 gataattgac ggagcgacct cgctttttgc tgttacgttc atcagaacct gatgctctgg
     7321 cgaagcgata tccagtccgg caagataatc gcgcattgcc tgaacgccgt cttccatcaa
     7381 acgcgtgtgc caggcaccgc tcacaccgag tttaaccggt tcgtatccag cggccatcag
     7441 cagcgtggta aattcattca acgcagcctg cgttccccca ataacctgct ggcgcggcgt
     7501 gttatcacag ctaatatcca gcgcaatgcc tgattccgta atcatcgtct gcagttgttc
     7561 gcggttgatg cctttaaccg c
//
LOCUS       DAZSNM010000194         7565 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZSNM010000194 DAZSNM010000000
VERSION     DAZSNM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.194"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(88..1559)
                     /gene="yeeO"
                     /locus_tag="ACHW42_003426"
                     /pseudo
     CDS             complement(88..1559)
                     /gene="yeeO"
                     /locus_tag="ACHW42_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            1720..1795
                     /locus_tag="ACHW42_003427"
     tRNA            1720..1795
                     /locus_tag="ACHW42_003427"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1753..1755,aa:Asn,seq:gtt)
     gene            complement(1833..2783)
                     /gene="cbl"
                     /locus_tag="ACHW42_003428"
     CDS             complement(1833..2783)
                     /gene="cbl"
                     /locus_tag="ACHW42_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR8945099.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2885..3802)
                     /gene="nac"
                     /locus_tag="ACHW42_003429"
     CDS             complement(2885..3802)
                     /gene="nac"
                     /locus_tag="ACHW42_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR8945100.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4130..4205
                     /locus_tag="ACHW42_003430"
     tRNA            4130..4205
                     /locus_tag="ACHW42_003430"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4163..4165,aa:Asn,seq:gtt)
     gene            complement(4261..5196)
                     /gene="ldtA"
                     /locus_tag="ACHW42_003431"
     CDS             complement(4261..5196)
                     /gene="ldtA"
                     /locus_tag="ACHW42_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR8945101.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5258..6337)
                     /gene="cobT"
                     /locus_tag="ACHW42_003432"
     CDS             complement(5258..6337)
                     /gene="cobT"
                     /locus_tag="ACHW42_003432"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR8945102.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6349..7092)
                     /gene="cobS"
                     /locus_tag="ACHW42_003433"
     CDS             complement(6349..7092)
                     /gene="cobS"
                     /locus_tag="ACHW42_003433"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR8945103.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7089..>7565)
                     /gene="cobU"
                     /locus_tag="ACHW42_003434"
     CDS             complement(7089..>7565)
                     /gene="cobU"
                     /locus_tag="ACHW42_003434"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR8945104.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2086 a         2048 c         1639 g         1792 t
ORIGIN      
        1 ggcgaatatt agcgatgccc acccacaatg tcaaagcctg ttttttaaat ttgaaatcgt
       61 ttgctgaaat aatctgcatt ttgtcgttta ttccgacaca actggctttt tttcacactt
      121 ttgcggctcg gatcgagggt atttccacag ccaacgtcca gtaaccattc gccagtaaaa
      181 cagcacggcc cgcacagccc agtcggcaaa cattcccatc cagacaccaa ccacacccca
      241 gccaagcatg attccctgca cataaccgac tacaacccgg caaccccaca tgctcaacat
      301 agaaacccac atggcgtaac gggcatcacg agcaccctta aatccagcgg gtagcaccca
      361 tgaggcggac caaataggca taaataaagc atttagctaa atcagaatca caacgacgtg
      421 tttaacctgt ggatcctggg tgtaaaacga tgccataacc ccggcaaagg gagccgttag
      481 ccaggcgatg gccgttaatc caagagtgga cagccagaac acatgccgca actgaatctc
      541 tgcttgcgct atctgcccta cccccaacct tcggcctgta atgatcgtag aagcagaacc
      601 gagcgcactt ccgggtaagt tgataagagc cgcaattgag aacgcgataa aatttccggc
      661 aataacgctg gtccccatcc cggcaacgaa catttgggtt aataaccgac cactggtaaa
      721 taacactgat tcgacactcg ctggaatacc aatccccatg acttcccaga taatgctaaa
      781 attcagcggt ttaaaatagc tctttaacga aatccttagc gcaggattaa aaccaatcgc
      841 cagcacccac aaaattgcaa ctgcgccaat ataacgagaa atggttaaac ccaacccagc
      901 cccgaccagg agaaaaggcc gtaaatcaat atgccgctaa taataatatt aagaatattc
      961 aggctaccgt taatcagtag cggtattttc gtattccctg caccacgaag tgccccgcta
     1021 ccaataagag tgatggcagc ggctggataa ctgagtacgg tcagctccag ataagtcaac
     1081 gccagtgctt taacttctgt cgtggcatca cccgcgacga aatcaataat ttgttcgcca
     1141 aaatgatgaa ttagcgttgc caacagtacg gcaaacaacg tcatgatcac caatgactgc
     1201 cgcgtcgcca ccctcgctcg tcgtcgatcc cgcttaccga gactaaatgc tacaacgaca
     1261 gtagtaccaa gatcgatagc tgcaaaaaaa gccataatga ccatattgaa gctgtccgcc
     1321 aaccccacgc cggccatcgc atcttttccc agccaactga ccagaaaagt gctcaggacc
     1381 cccatcaaca ggacacaggc gttctccatg aagataggaa cagcaagcgg ggttatctcg
     1441 cgctagaaca acactttgta gctcttgcgt ttagagtgcc agcgagtgcc gtgaacaacc
     1501 tggcgtaaag cagaggagat attcaaagcc gaccttaatt gcagaaagtg aaaccacatt
     1561 tcaaatgatg agggagaatc agcaaagctg caaagatttt cgccaacaaa ttgtctgcaa
     1621 atgcaacaaa ctgttgatag aaacggcaaa cagttgggaa atttaaaaat cgggtttgac
     1681 aaaatatttt tcgccgttaa gatgtgcctc aacagcgatt cctctgtagt tcagtcggta
     1741 gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattt
     1801 ctgaaaagcc cgcttttata gcgggctttt tgctatatct gataatcaat ttcctcttca
     1861 ctgttttcca tcacctggcg cttgatatcc tcaactgaca gtcctgcatt acaaagttcc
     1921 agaaagcgcc aaacatagtt acgctgaagt tgtcctcgct tcagtcccaa ccagacagta
     1981 ttagcatcga aaagatgccg cgtatccagg cggattaaat tctcttcctc ttgttcgccg
     2041 ctggattgtt cggcaactaa tccgatccca agcccaagag caacataggt tttaatgaca
     2101 tcagaatcct gtgcacttaa tacaatatct gccggcaaac ctttgcgggt aaatgcgtca
     2161 tcagtgcgtg agcgccccgt aattccctgt cggtaagtaa ttaacggcca cttcgctatt
     2221 gattccagcg tcaatggtgt aatttgcgtt aagggatgat cgagtggaac aagcaaacta
     2281 tggtgccaac aaaaccatgg gaaggcgacg agctgcgggt cattactcaa acgctcgctg
     2341 gcgataccaa tatcagcttc gccattttgc aacaacgtcg caatttcctg tggcgaaccc
     2401 tggattagct cgagccgaac ctccgggaaa agttcgcgaa aagctttaat gacctctggc
     2461 aagctataac gtgcctgagt atgcgtcgtt gcaatagtga gaacaccaga cgtatcgttg
     2521 gtaaacaggt ctgcaagccg acgaacatta ctggcttcat tcagaatacg ttctgcaatg
     2581 accagtaatg ctttgcccgg ttcagtcatc cccagcagtc gcttacctcg tcgaacaaat
     2641 atttcgatgc caaattcatc ctccagttcc cgaatatgac ggctaacgcc tgactgtgag
     2701 gtaaaaagca tattcgcaac ctctgtcagg ttgtaatcct gacgtgcagc ctcgcggatt
     2761 atctttagtt gttggaaatt cacggtaaac tccgggcagt tcagatatcc cgttattgtt
     2821 aaagtctaat gcccggcata acaaataata aaaacccgca tcttattcca tcccgatata
     2881 acgcttagct caccaattgc cactgccttt tttccatcac tggagaacta atcactgaca
     2941 ttaacaactc tttcactgcc tgtgcctgtg gtgacaagtt tgctctggcg ggtaaattta
     3001 aggacaaaga gagactcatt gaaggagtgg taatgcgtga catccaccca tttactgcgc
     3061 cacataacga acgcgcagcc gattcaggta atactgcaac gcccatgccg ctggcaatcg
     3121 ctgcggtaag cgtggcaata gactcaattt caccaatgac ttttgccgtg agccgccgta
     3181 gggaaaaagc ctcatcaaca cgaagtctaa cagcgctgta atcactgggg agaaaaaggt
     3241 tcatttgcgc aatagcatta acatcaacgt tttgccccgg gcaatcttga gttcctacca
     3301 ggaaaagatc ttctttcagc aaagcctgac tggatacccc agccacaggg gaatgctcat
     3361 aaatcaccgc catatcgagt tggtgattta tcagtttttc gttaagcact gcaccactat
     3421 tttcatgaag atagataacg acctccggga attcagcgcg aaccgcctgt aataagggca
     3481 tggtgatgga tgacgcagcg gttcctggcg caaagccaat cgagacttgc cccgataatg
     3541 actgaccaac gttatgcacc gccagttggg cctgctcaca ctgacgtaaa atggcccgtg
     3601 catgggtata gagaattttt ccggcgtctg ttggcgtaac gccccgtttt gtgcgaatca
     3661 aaagctgttg atttaactca ccttccagta tggcaacctg ttggctgagc gctggttgtg
     3721 cgatatgcaa tacttcagca gcctgggtca ggctaccaat atctacaatt tttacgaagt
     3781 atttcaggcg tctgaagttc atgttgcctc cggtttttaa gaatcggccc aagtgccgcc
     3841 attacttaca accagtatgc aagatgcctg ccagttctat tttggcgttg atgcgcaagc
     3901 taaccaactg tcaaataaga gattatgata gattcgtcat tggccctttt tatcagcagt
     3961 cgcattcacc tgccctataa aaggagggta tgcaccacga tggttcatta cccaataaga
     4021 ttgaaagctc accactttgt tgaaattggc agcaaacaaa caaaaaaaat gcatttcacc
     4081 ctttgacatc accatgcact gccattaata tgcgccccgt tcacacgatt cctctgtagt
     4141 tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg
     4201 agccaaattc aaaaaagcct gctttcgagc aggcttttta ctttctaatt accaacgctc
     4261 ttaaaacatc tgtcttgaac cagaactaat ttgcacaggc attcccgatc gatgttgcaa
     4321 cgcagcattt gcgcgattta catcaacttc ttgcccgttg ataaacgccc gcaaagatgg
     4381 ggttaccggc aatggcactt ttcggtcaga ctcatattct gcacgattgc gcgacagcgg
     4441 ctcatgaact tccagccact ttgaaccatc cggttccgtt gtgtatttca ctggctgatc
     4501 aataatttgt acacgagtgc ctacaggaac attatcaaac agatatttga tatcgtcatt
     4561 acgcagacga atacagccct gacttacacg gagcccaata ccaaaattgg cattggtacc
     4621 gtggatggca tacaacctgc caatataaat cgcgtacagc cccatggggt tatcgggccc
     4681 cgcaggaaca aatgcgggca aactctcccc tcgtttcgca tattcgcgcc ggatgttcgg
     4741 cgttggcgtc caggttggtg cttcttgttt acgttcaacg gtagtcaccc agttacgcgg
     4801 ggtttctcgc ccagcctggc cgataccaat aggaaagact tccactgtat tactgtctgg
     4861 cggatagtaa taaagacgca tttcagcgac gttaaccaca atccctttac gaacagtggc
     4921 gggcaaaatc agttgctgcg gaatggtgag ttgtgagcca ggcttcggca aaaaaacatc
     4981 agcgcccgga ttcgcttcca gcatattact taacccttgc ccgtattgtg cggcaaaagt
     5041 ctccagcggc tgagtattgt gatcaggaac agttacagta agcgactgcc ccactaaacg
     5101 gctaccctct ggaggtaatg gataagttac cgccaggcta ttttggctgg caaaaagcag
     5161 agcaaatgag caaagaatat ttacacgacg catcatgtcc ctttcctaag tcgcgaatac
     5221 tatccgataa gtatagcttt tatcagactt ttcgttttta gctgttcaaa tcagaagtcg
     5281 tattccccgg tagaacaata ttactggcag caagttcgcc catgttgttg tatatcgcac
     5341 aagcagcttc gatgatggac atcgccagag ctgcaccact cccctcacct aaacgcatat
     5401 ccatattgag ataaggctcc agccccaaat gcgagagcgc tatacgcgcg cctttttctg
     5461 ccgacaagtg agaaggaatg agatacggtt tgattgcagg agacatctgg caggctgcga
     5521 gcgcagcagc ataagaaaga aatccatcca gcaacacggg taaaccgcag gaagcagcac
     5581 ctaacatcac tccagctatt ccgaccaaat caaatccacc cacttttgcc aggacatcaa
     5641 caccatcctg aggatttggt tgattcaacg taatcgcccg acgcacaaca tcaattttat
     5701 tagccagttt atctgtcggc aggtttgcgc caatcccaac cacttcttca ggatcccggc
     5761 cagtgattgt gctgactatt gccgctgccg gtgtcgtgtt tgccatcccc agttcaccta
     5821 caccaaacag cgtgacaccg tttttcgcca gctcccgcgt ataacatatg acgtccaaaa
     5881 gcaacttttc agcctgacgg cgactcattg ccggagctga agcaatattg ccgctacctc
     5941 gtgcgacacg catgttgata agcccgggga taggctcagc agtatcaata ccaacatcaa
     6001 ttacgtggac gttagcgccc gcttgtgctg ccagcacaca cacgccggtt gttccacggg
     6061 tcatattttc agcctgtatg gctgtcactt cttttggaga aatagcgacc ccttcctccc
     6121 agacgccgtg atcggcacac ataaccagta ccgctttttt gcccacatgc ggtatgccat
     6181 tcacccccgg cattcctgcc agttgtatgg caagcgcctc cagctttcct aggctaccaa
     6241 caggtttgaa taacccgtca acatgccgtt gtgcacgcga catagcggca gaattgatgg
     6301 ctgggatcgt attcagtaaa tcggcaatta tttgcatgtc acgtttcttt atagcagtgc
     6361 cagcaggaag accagttcac caagctctat ggctgctccc agcgtatcgc ccgtttgccc
     6421 gcccagcgta cgtttgagaa gctgaccgag gatgaaaatc gccaccatcg tcaccaccat
     6481 agcagccaca ccatgcatac ccagcaataa tacggcagca aaaatagcgg ctaagccgag
     6541 tgtgacgcag gtttgtcgcc catcaatttt gccaatgaac acattgccaa gcccttcttc
     6601 ccgcgcataa cgatgacgat acatcaataa tgcggcagtc ccgcggctga ccgcacatgc
     6661 tgccgccagc gatgcaagga tcggttcgcc acgcagagcc aactcgctta acaccagaat
     6721 ctttgccagt accacaaaaa tcaatgccag accgccgtgg gtgcctaaac ggctatcacg
     6781 catgatttcc aacatgcgat cgcgactacg tgcagaaaat acgccatcac aggtatcagc
     6841 gaggccgtcc agatggaatt ccccggtcat cagcgccagc acaagtacgc taaacagtgc
     6901 cgccagtggt gcgccacacc atgcctgcag caccatgaag accagcccgc taatcgcgcc
     6961 aagcaataat ccaatcaaag gaaaagtaat aataccgcga gaataatgtt cgaaatccag
     7021 tccctgggac cagcgacgcg gtacaggcag acgcgtaata aatgagagca tcgcccaaaa
     7081 taatttgctc atttgatttt tactccaata cccgaaacca ccagccatac ttcatttgcc
     7141 gcagcggcca actgctgatt tacccgcccg gcaatatcac gaaaatgtcg tgccagacga
     7201 ctctccggca caatccccat tcccacttcg ttagtcacta atacaacctt tgcggggcaa
     7261 cgttggcagg cagcaatcaa cgactgaatc tcagcattaa tcgcctgttc catcgcctga
     7321 taatcccatt catcagggtc tttatcgccg ccataatcaa acaacagatt agtcaccatt
     7381 gtggtaacgc attccagcaa cacagcctca ttcgggttaa tgtctgcatg aattaattca
     7441 tcaagatgtt gccagcgctc cactgtgcgc cagtgctccg ggcggctttg ccgatgatgt
     7501 tctatccgtg cagccatctc atcatcaagg atttgcgagg tagcgatata cagaacctgt
     7561 gaaga
//
LOCUS       DAZSNM010000195         7508 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZSNM010000195 DAZSNM010000000
VERSION     DAZSNM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.195"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            252..1649
                     /gene="ulaA"
                     /locus_tag="ACHW42_003435"
     CDS             252..1649
                     /gene="ulaA"
                     /locus_tag="ACHW42_003435"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFR8945105.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1665..1970
                     /gene="ulaB"
                     /locus_tag="ACHW42_003436"
     CDS             1665..1970
                     /gene="ulaB"
                     /locus_tag="ACHW42_003436"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR8945106.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1980..2444
                     /gene="ulaC"
                     /locus_tag="ACHW42_003437"
     CDS             1980..2444
                     /gene="ulaC"
                     /locus_tag="ACHW42_003437"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR8945107.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2458..3108
                     /gene="ulaD"
                     /locus_tag="ACHW42_003438"
     CDS             2458..3108
                     /gene="ulaD"
                     /locus_tag="ACHW42_003438"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR8945108.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            3118..3972
                     /gene="ulaE"
                     /locus_tag="ACHW42_003439"
     CDS             3118..3972
                     /gene="ulaE"
                     /locus_tag="ACHW42_003439"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR8945109.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            3972..4658
                     /gene="ulaF"
                     /locus_tag="ACHW42_003440"
     CDS             3972..4658
                     /gene="ulaF"
                     /locus_tag="ACHW42_003440"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR8945110.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4787..5062)
                     /gene="yjfY"
                     /locus_tag="ACHW42_003441"
     CDS             complement(4787..5062)
                     /gene="yjfY"
                     /locus_tag="ACHW42_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR8945111.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5389..5784
                     /gene="rpsF"
                     /locus_tag="ACHW42_003442"
     CDS             5389..5784
                     /gene="rpsF"
                     /locus_tag="ACHW42_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR8945112.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5791..6105
                     /gene="priB"
                     /locus_tag="ACHW42_003443"
     CDS             5791..6105
                     /gene="priB"
                     /locus_tag="ACHW42_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR8945113.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            6110..6337
                     /gene="rpsR"
                     /locus_tag="ACHW42_003444"
     CDS             6110..6337
                     /gene="rpsR"
                     /locus_tag="ACHW42_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR8945114.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6379..6828
                     /gene="rplI"
                     /locus_tag="ACHW42_003445"
     CDS             6379..6828
                     /gene="rplI"
                     /locus_tag="ACHW42_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR8945115.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6899..7462)
                     /locus_tag="ACHW42_003446"
                     /pseudo
     CDS             complement(6899..7462)
                     /locus_tag="ACHW42_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         1797 a         1813 c         2047 g         1851 t
ORIGIN      
        1 ttgccaggga agttgttgtt gattttgagt ggggaaagat ttaatggaat gtgtagttaa
       61 attaactaaa tgaatttaaa taaataattg tttcgttgtg tgaatcccac tctatccatg
      121 tggaattatt tgcgggtcgc gtcacattta atcataaata atcttgttgt gattactttt
      181 gaaaattaga gtgagtgcac aacattccgg gtgtgcggaa tacccggtta cctcttcttc
      241 aggagatcgt tatggagatc ctctacaaca tctttaccgt gttttttaac caggtcatga
      301 ccaatgcccc gttgttgctg ggtattgtga cctgtctggg ctacatccta ctgcgcaaaa
      361 gtgtcagcgt tattattaaa ggcacgatta aaaccataat tggtttcatg ttgttgcagg
      421 cagggtccgg catcctcacc agcaccttca aaccggtggt ggcgaaaatg tccgaagtct
      481 acggcattaa cggcgcaatt tccgatactt acgcgtcgat gatggcaacc atcgaccgca
      541 tgggcgatgc ctatagctgg gtaggttacg cggtgttgtt agcgctggcg ctgaacatct
      601 gttacgtgct gctgcgtcgc attaccggta ttcgtaccat catgctgact ggtcatatca
      661 tgttccagca ggcggggctg attgccgtta cgttgtttat tttcggctac tccatgtgga
      721 caaccattat ctgcacggcg attctggttt cgctctactg gggcattacc tccaacatga
      781 tgtacaagcc gactcaggaa gtgacggacg gctgcggttt ctccatcggt caccagcaac
      841 agtttgcatc attaattgcc tataaagttg cgccgttcct cggcaaaaaa gaggaaagcg
      901 ttgaagacct caaactgcca ggctggctga atattttcca cgacaacatc gtctccacgg
      961 cgattgtcat gaccatcttc tttggtgcca ttctgctctc cttcggtatc gacaccgtgc
     1021 aggcgatggc aggcaaagtg cactggacgg tctatatcct gcaaaccggt ttttccttcg
     1081 cggtggcgat cttcatcatc actcagggtg tgcgcatgtt tgtggcggaa ctctctgaag
     1141 cgtttaacgg tatctcccag cgcctgatcc ctggtgcggt tctggcgatt gactgtgcgg
     1201 ctatttatag cttcgctccg aacgccgtgg tctggggctt tatgtggggc accatcggtc
     1261 agctgattgc ggttggcatc ctggtcgcct gcggttcctc gatcctgatt atcccaggct
     1321 ttatcccgat gttcttctcc aacgccacca tcggcgtgtt cgctaaccac ttcggcggct
     1381 ggcgtgcggc gctgaagatt tgtctggtga tggggatgat cgaaatcttt ggttgcgtct
     1441 gggtggtgaa acttaccggt atgagtgcct ggatggggat ggcggactgg tcgattctgg
     1501 caccgccgat gatgcagggc ttcttctcca tcggtatcgc ctttatggcc gtcatcattg
     1561 taattgcact ggcttatatg ttcttcgctg gccgcgcact gcgcgcagaa gaagatgcag
     1621 aaaaacaatt ggcagaacag tctgcttaat aaggagtttt gattatgacc gtacgtattc
     1681 tggctgtgtg tggcaacgga caaggcagtt ccatgatcat gaagatgaaa gtggaccagt
     1741 ttttaaccca atcaaacatt gaccacacgg taaatagctg cgcggttggc gaatacaaaa
     1801 gcgagttgag cggcgcggat atcatcatcg cttctacgca cattgcgggc gaaatcaccg
     1861 tgaccggcaa caaatacgtg gttggcgtgc gcaacatgct ctctcctgcc gactttggcc
     1921 cgaaactgct ggaagtgatc aaagcgcatt tcccgcagga tgtgaagtaa ggacgcgcca
     1981 tgaaattaca tgattcgctg gcggagaata aatccatccg cctgcaggct gaagctgaga
     2041 catggcagga cgcagtaaaa atcggcgttg acctgctggt ggcggcggat gttgtcgagc
     2101 cgcgttacta ccaggcgatt ctggatgcgg ttgaacagca cggtccgtac tttgtgctcg
     2161 caccaggcct ggcaatgccg cacggtcgcc cggaagaggg cgttaagaaa accggttttg
     2221 cgctggtgac gctgaaaaag ccgctggagt tcaaccacga agataacgac ccggtggata
     2281 tcctcatcac catggcggcg gtcgatgcca acacgcacca ggaagtgggc atcatgcaga
     2341 tcgtcaacct gtttgaagat gaagaaaatt tcgaccgttt acgtgcctgc cgtaccgagc
     2401 aggaagtact ggatctcatt gaccgcacca acgcggcagc ttaagaagga attgaagatg
     2461 tcattaccga tgttgcaagt cgcgctggac aaccagacta tggatagcgc ctacgaaacc
     2521 actcgcctga ttgccgaaga agtcgacatt atcgaagtgg gtaccattct gtgcgtgggc
     2581 gaaggcgtgc gtgcggttcg cgatctgaaa gcgctctacc cgcacaaaat cgtgctggca
     2641 gacgccaaaa ttgccgatgc gggcaaaatt ctttctcgta tgtgcttcga agccaacgct
     2701 gactgggtga ccgtgatttg ttgtgcggat atcaacaccg ccaaaggtgc gctggacgta
     2761 gcaaaagagt ttaacggcga cgtgcagatc gaactgaccg gttactggac ctgggaacag
     2821 gcgcaacagt ggcgcgacgc aggtattcag caggtggttt atcaccgcag ccgtgacgcg
     2881 caggccgctg gcgtggcgtg gggtgaagcc gatatcactg cgatcaaacg tctttccgat
     2941 atgggcttca aagtcaccgt caccggtggc ctggcgctgg aagatctgcc gctgttcaaa
     3001 ggcattccaa ttcatgtgtt tatcgccggt cgcagcatcc gtgatgccgc ttctccggtt
     3061 gaagccgcgc gtcagttcaa acgttccatc gccgaactgt ggggctaagg agcggatatg
     3121 ttgtccaaac aaatcccgct tggcatctat gaaaaagcgc tccccgccgg ggagtgctgg
     3181 ctggaacgcc tgcgactggc aaaaacgttg ggcttcgatt ttgtcgagat gtcggtagat
     3241 gaaactaacg agcgcctgtc gcgccttgac tggagccgcg aacagcgtct ggcgctggtg
     3301 aatgctattg ttgaaaccgg cgtgcgtgtg ccgtccatgt gcctttctgc ccatcgtcgt
     3361 ttcccgttgg gcagcgaaga tgacgcggtg cgggcgcagg ggctggagat tatgcgtaaa
     3421 gccatccagt tcgcccagga cgttggcatt cgcgtgatcc agctggcggg ctatgacgtt
     3481 tactatcagg aagccaataa cgaaacgcgt cgtcgtttcc gtgacggcct gaaagagagc
     3541 gttgagatgg cgagccgcgc gcaggtgacg ctggcgatgg agatcatgga ttatccgttg
     3601 atgaactcca tcagcaaggc gctgggttac gcgcactatc tcaacaaccc gtggttccag
     3661 ctctacccgg atatcggcaa cctgtcggcg tgggacaacg atgtgcagat ggagttgcag
     3721 gccggaatcg ggcatatcgt cgcggtgcat gtgaaagaca ccaaaccggg cgtcttcaaa
     3781 aacgtgccat ttggcgaagg tgtggtggat ttcgaacgtt gcttcgaaac gctcaaacag
     3841 agtggctatt gcgggccgta cctgattgag atgtggagcg aaacggcgga agacccggcg
     3901 gcagaagtgg tgaaagcgtg tgattgggtg aaagcgcgca tggccaaagc aggcatggtg
     3961 gaggcggcat aatgcaaaag ctaaaacagc aggtgtttga agccaacatg gatctgccgc
     4021 gctatgggct ggtgaccttt acttggggca acgtcagcgc tatcgaccgc gaacgcgggc
     4081 tggtggtgat caagcccagc ggcgttgcct acgaaaccat gaaagcagac gatatggtgg
     4141 tggtcgatat gagcggcaag gtggtggaag gggagtatcg cccatcttcc gacactgcga
     4201 cgcatctcga actctaccgt cgttacccgt cgcttggcgg cattgtccat acccactcca
     4261 ctcatgccac cgcatgggcg caggcggggc tggcgatccc ggcattaggc accacgcacg
     4321 ctgactactt ctttggtgat atcccgtgta cgcgtggatt aagcgaagaa gaggtgcagg
     4381 gtgagtatga actgaacact ggcaaagtga ttatcgaaac gctgggcaac gccgagccgc
     4441 tgcatacgcc gggaattgtg gtgtatcagc acggaccgtt cgcctggggg aaagatgccc
     4501 acgacgcggt gcataacgcg gtagtgatgg aagaagtggc gaaaatggcg tggattgcgc
     4561 gcagtattaa tccacaactc aatcatatcg acagctacct gatgaataaa cacttcatgc
     4621 gtaagcacgg tcctaacgct tattacgggc agaagtaaaa cacgcgctgc ggaaatttcc
     4681 ttcctcggga gataactggt ctaattccgc agccgttttt caaaaaaagc cccctgcgaa
     4741 gggggcaaag caaactatgg caatgtttcg ttggttatac ctggtgctag cgataaatat
     4801 ccgcgctggc gtgcatattg ccgttactcc ccggttcccg catcagaatt acgtggtagt
     4861 acgttgcgcc ttgcgcatct gtttcttcat ttaatgcctg acgtgcttcg ctttcagtgg
     4921 cgaaattatg attgatataa atcacgccta agctttgcac atcgtccata tttctggcct
     4981 ggtggttatt aatttcaatg gctgcccatg tatttgcact tagcaaaagc acagccagaa
     5041 gggctaaaac tcgactgaac atagatacct cctcgacggc tgactttgtg tgctctcctc
     5101 tgtgatgatc ttctgattta attttaatca atgataaaga agttgatggt gaccatttct
     5161 gatgcagttg ttcaaaaaaa cactgtgacg gaatgtgatg aacttcaaat cagcggttta
     5221 gaggttattt gcgaaagggg agatttattt ctgctccgcc cttgagttta gcggggtatg
     5281 ccagtactat aacgcgtcat ttttcagccg acctttaaca cgttccttgc ctccccggga
     5341 ttcggctgac ccagacagga ggctgaataa tccgtaagga gcaattcgat gcgtcattac
     5401 gaaatcgttt ttatggtcca tcctgaccag agcgaacagg ttccgggcat gatcgagcgc
     5461 tacactgctg ccatcactgg tgcagaaggc aagatccacc gtctggaaga ctggggccgc
     5521 cgtcagctgg cttacccgat caacaaactg cacaaagcac actacgttct gatgaacgtt
     5581 gaagctccgc aggaagtgat cgatgagctg gaaactacct tccgcttcaa cgatgccgtt
     5641 atccgcagca tggttatgcg taccaagcac gctgttaccg aagcatctcc gatggttaaa
     5701 gcgaaagacg agcgccgtga gcgtcgcgat gatttcgcaa acgaaaccgc tgatgatgct
     5761 gaagctgggg attctgaaga gtaatttctg atgaccaacc gtctggtgtt gtccggcacc
     5821 gtgtgcagga ctccccttcg aaaggtcagt ccatcaggaa ttcctcactg ccagttcgtg
     5881 cttgagcatc gttctgtgca ggaggaagcc ggctttcacc ggcaggcatg gtgtcaaatg
     5941 cccgttattg ttagcggaca cgaaaaccag gccattactc acagtataac ggtcggcagt
     6001 cgcataaccg ttcaggggtt catttcatgc cacaaggcaa agaacggact gagcaaaatg
     6061 gttttgcatg ccgagcagat tgaattgata gattctggag actagccata tggcacgtta
     6121 tttccgtcgt cgcaagttct gccgtttcac cgcggaaggc gttcaagaga tcgactataa
     6181 agatatcgct acgctgaaaa actacatcac cgaaagcggt aagattgtcc caagccgtat
     6241 caccggtacc cgtgcaaaat accagcgtca gctggctcgc gctatcaaac gcgctcgcta
     6301 cctgtccctg ctgccgtaca ctgatcgcca tcagtaatcg gtcacggtcc attaatacga
     6361 ctttgagagg ataaggtaat gcaagttatt ctgcttgata aagtagcaaa cctgggtagc
     6421 ctgggtgatc aggtaaacgt taaagcgggc tatgctcgta acttcctggt accgcagggt
     6481 aaagctgttc cagctaccaa gaaaaacatt gaattcttcg aagcacgtcg cgctgaactg
     6541 gaagctaaac tggctgaagt tctggcagct gctaatgctc gcgctgagaa aatcaatgca
     6601 ctggaaactg ttaccatcgc gtctaaagct ggcgacgaag gtaaactgtt cggttccatc
     6661 ggtactcgcg acatcgctga cgctgtaact gcagctggcg ttgaagtggc taagagcgaa
     6721 gttcgtctgc cgaacggcgt tctgcgtacc actggcgaac acgaagtgag cttccaggtt
     6781 cacagcgaag tattcgcgaa agtgatcgta aacgtagtag ctgaataatt cgttattcaa
     6841 cgagacgtaa aaacgccgac cattggtcgg cgttttgctt tctatttttc gtcaggcatt
     6901 aatttcgcaa gtagatccat tcaccattct cttgtgtcca taagtcagga tggtttgctg
     6961 gcgttagatg catacgatca ctccttatgt ctcggtcctg cggcactcgc tcgaaaccta
     7021 aacccgagta gattgcggct gaatttgata aggaacgtag accaattttc aaatcatggg
     7081 gtaaatcaga atacgcgttt agcaatgcag caatgatgaa tccgagggca ttagaaacgc
     7141 ctgtaacagt gtgaagttgg tcgatataaa gatcgattgt tggtagttca gatgagcagc
     7201 aattaggcat ttgggcagga agggttacaa cgtatcctat cgtttggccg gtatagtctg
     7261 gagtactata accaggtccc aaaacaacct cgtgtattgt gtatttactg ttgggttcga
     7321 aggattcgac atcttgcaat aaatatttca gttctgcggt taacctcgca cccgcgtaag
     7381 ctttattgta tttctgggcg tgaatattat ctaatgtagc ttcatggttc catccaaaag
     7441 aattatcacc attcattgtt actgaatcgc cacgggttta acagacacct cagagtcatt
     7501 taagatgg
//
LOCUS       DAZSNM010000196         7469 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZSNM010000196 DAZSNM010000000
VERSION     DAZSNM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.196"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="ACHW42_003447"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="ACHW42_003447"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR8945116.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /gene="malI"
                     /locus_tag="ACHW42_003448"
     CDS             complement(721..1749)
                     /gene="malI"
                     /locus_tag="ACHW42_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR8945117.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /gene="malX"
                     /locus_tag="ACHW42_003449"
     CDS             1924..3516
                     /gene="malX"
                     /locus_tag="ACHW42_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR8945118.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="ACHW42_003450"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="ACHW42_003450"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR8945119.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4802..5803
                     /gene="add"
                     /locus_tag="ACHW42_003451"
     CDS             4802..5803
                     /gene="add"
                     /locus_tag="ACHW42_003451"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR8945120.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5839..6879)
                     /locus_tag="ACHW42_003452"
     CDS             complement(5839..6879)
                     /locus_tag="ACHW42_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR8945121.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7122..7247
                     /gene="blr"
                     /locus_tag="ACHW42_003453"
     CDS             7122..7247
                     /gene="blr"
                     /locus_tag="ACHW42_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR8945122.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
BASE COUNT         1772 a         1938 c         1836 g         1923 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgctattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccacctc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cggcgttgtt aaccaggata tcaactttac ccagattact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccaggc
      421 aaatgcctga ccatccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgtgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccattaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctac caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4201 gcgttgctga catcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc aatcgtggat
     4741 ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa agagtgcgac
     4801 catgattgat accaccctgc cattaactga tatccatcgc caccttgatg gcaacattcg
     4861 tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg cacaatccct
     4921 tgaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg tgagctttct
     4981 gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc gccgcgtggc
     5041 atttgaaaac attgaagatg cagcccgtaa cggcctgcgc tatgtcgagc tgcgtttttc
     5101 accaggctac atggcaatgg cacataagct gcctgtagcg ggtgttgtcg aagcggtgat
     5161 cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta tcggcattat
     5221 gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt tagcccaccg
     5281 tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg gaagtctgtt
     5341 cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc atgcaggcga
     5401 agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg agcgtattgg
     5461 acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg agcaacaaat
     5521 tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag agctggctgc
     5581 acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca ctgacgatcc
     5641 cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag ctgctgggtt
     5701 atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt tcctcagcgc
     5761 agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat ggatggtgca
     5821 aattgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg cggattcgat
     5881 gcccagctca atcaactcca ttacctggat tgcctggctt gccggaaccg gattttcacc
     5941 atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc caggcaccgt
     6001 caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc catcacgcat
     6061 atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt cttcctgtgg
     6121 atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc gtgctgactc
     6181 agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg acaagatggc
     6241 gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta ccgtcatgct
     6301 gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta aatcgtacca
     6361 gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg gacggaagcg
     6421 gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga gcaaaccttt
     6481 tagcgtcaag aaatcgctat cccaacggcg gttatggaat acagacagca cacgccccag
     6541 gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa agggtttatc
     6601 aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat gggtatcgtt
     6661 gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct tcggctcaga
     6721 gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc tggagattac
     6781 cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt tgctcgcata
     6841 accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc tcattgaagt
     6901 atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac ctgccagcaa
     6961 taagggatgt tgtttaactt aagtcaaaaa aatagcgaat tttccaacga caaaagctaa
     7021 atatcgcaaa aacctcagta aaaatcttgc tggagctatt attgctaagt aacatttacc
     7081 ccctgaagtt aatggatcaa tcaagagaga tgtgggctgt aatgaatcgt cttattgaat
     7141 taacaggttg gatcgttctt gtcgtttcag tcattcttct tggcgtggcg agtcacattg
     7201 acaactatca gccacctgaa cagagtgctt cggtacaaca caagtaagct ctgcacttgt
     7261 ggagcgacat gctgcccgtc cgggtgcatg ttttcacttg tcggatatta aaccaggaat
     7321 ttattatctt gttcgatgtt gttggtgatt gtcagggata gtaaagcgcg ttactctttt
     7381 cagagcagtt tttcatccct gaatatttgc catcagcgca ttcttattat tgtaataaca
     7441 caagcgttca cttttggtga aattttatc
//
LOCUS       DAZSNM010000197         7446 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZSNM010000197 DAZSNM010000000
VERSION     DAZSNM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.197"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            164..649
                     /locus_tag="ACHW42_003454"
     CDS             164..649
                     /locus_tag="ACHW42_003454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945123.1"
                     /translation="MRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIMPETKFTLLNVT
                     K"
     gene            646..1032
                     /locus_tag="ACHW42_003455"
     CDS             646..1032
                     /locus_tag="ACHW42_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945124.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1057..3270
                     /locus_tag="ACHW42_003456"
     CDS             1057..3270
                     /locus_tag="ACHW42_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFR8945125.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTHDVSE"
     gene            3293..3738
                     /locus_tag="ACHW42_003457"
                     /pseudo
     CDS             3293..3738
                     /locus_tag="ACHW42_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3760..7318
                     /locus_tag="ACHW42_003458"
                     /pseudo
     CDS             3760..7318
                     /locus_tag="ACHW42_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459289.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         1884 a         1755 c         2188 g         1619 t
ORIGIN      
        1 aacaaatatt gatggcaata tcattataca taaggatggt aggaggacgt atttattaaa
       61 tcaaaatact ggttcgatta cggctgtgat aagatggaag tatgagtagt ataaggataa
      121 taccgttaca gatgactgga ctatatggga aaaagctatt tttatgcgag cgatatattt
      181 atctgttcag caggcatgga atgggaaaat aacttattct gtttccggtg aatctgagtt
      241 cgctaaaaaa tttcagggta aagctttgcc ttttgatgta agaataattt ctgcatctca
      301 gaatgaagac tggttggtta ttgcaacaaa ggttttgcct ggtgctgatt taagaacata
      361 tgttgatttt aaaaacagta cggttcatgt tgattcagcg ggcctggaaa aagtagcgaa
      421 atgtattaac tgtaataata cgttgcaagt aaatatacct catgaagccg gacatgtact
      481 tggttactta gatgatgatt atgattcttc gtctccgtat gttggagata ttagtgggtt
      541 gatgaatgtg ggaatggagc tttgggaacg ataccttaaa aacgcaacta taacgttgaa
      601 tatcataatg cctgaaacta aatttacttt attgaatgta accaaatgaa atttttctgt
      661 attatagtga ttttcctttc aataaattac atagctatag tgaatgtttt caagtcttct
      721 gcagttgctc aatccaccaa atattatgaa gaaaatgata tacatgatgg aagtaatgac
      781 atctttttta tgaagaaata tgggccgcct gctcatgagt tcgtgaggcc acgatataaa
      841 tttgttggtg agtattatat cggcttgaat caaacttatc gcataacaga tcctgaattt
      901 caagatgttc ctattaagga aatgttctgg aatttgcagg gggatttgaa tctgacatgt
      961 tggtttcatt ataaagacgg gcaatggaga gtgattactt atattttttg gccaccaggg
     1021 gctagatttt aatccggtat taaaggagca aatatcatga gttttgtatc tacgggtaat
     1081 aaaccagttg gaaacggtgg acccgtcatc accacaccac caatagctgg cgaaagtggc
     1141 ggtatgagca caggttcagc tgtaaccgat gtatctggtg ccgccgagga gatggcagaa
     1201 caggctgcgg cggacctgtt tggtgcgctg ccggaaccgt caggtctggt taaagccgca
     1261 gtagctgctg cgcaggctgc cgctgcagca gcagggatct cggatatggc tggtgcagtt
     1321 caggatgcag cggcgtcgct tgcagccggt gctccggggg cgcataacgt cacagtaagt
     1381 ggtagtgcgg tgccgccgca gatgcttttg tttgcaggca tgaatggttc ggaagggctt
     1441 gggaatctgt tttcttacac agtacagctt aaaacgctgg acgccctgaa cctggggtac
     1501 gtttcccccg ctgctaacct gccgcttcag ctcatggtgg gcaaagacct gtgcgtcagt
     1561 atcgaactgg acggcggcgg aaaacggtat atcagcgggc tggtgacggc ggcgagggtg
     1621 gtcggccatg agagccgttc ggtaacgtat gaattacgca ttgagccatg gctgaagctg
     1681 ctgacccata ccagcgacta caaagcgttc cagaacaaaa ccgtggtgga tattctcgat
     1741 gaagtactgg atgaatattc ctttccggtg gaaaagcggc tggtggaaaa ttatccaccc
     1801 cgcgcctggc aggtgcagta tggtgaaacc gacttcgatt tcatccagcg cctgatgcag
     1861 gaatggggga tttactggtg gtttgagcac agcgagaaca gccacacgct ggtgctggtg
     1921 gatgccatca atggtcataa agcgtgccct gactcgccgc tggtggagtg gcaccaggaa
     1981 gggctgaaac tggataagga atttatccac accattacgg ccagtgagag gctgcgtact
     2041 ggaaagtggg tgatggacga ttttgatttt atgaagcctc gttcgctgct taaaagtacc
     2101 gtggccagtc cgcgtgatac cggtcatgct gaatacgagc attacgaatg gcctggtgat
     2161 tacttcacta cgggcgaagg cgagatgctg acgcgcattc gtatggaagc gcagcgcagc
     2221 ccaggcagcc gggcacatgg ggccggtcat atccgtaccc tcatgaccgg gtacaccttt
     2281 acgttgatga atcacccgac ggcagaaatt aatcaggaat atctgctggt acagaccact
     2341 ctgtttttac gggataatgc gcagcacagc ggtcaggatc agcattttac ctatgtcacc
     2401 acgtttgaac tgcaccccac cagcgaagtt taccgcccac aacgaaccct gagcaaaccc
     2461 cacaccaaag gcccgcagag cgccatcgtc accggcccgg cggggcagga aatctggacg
     2521 gataaatatg gccgggtgaa ggtgcagttt ggctgggacc gttacggaaa gaatgacgaa
     2581 aacagttcct gctggatccg cgtcagctat ccgtgggcgg gtaaaggatt cggcatgatc
     2641 cagatcccgc gtattggcca ggaagtactg gtggatttca aaaacggcga tccggatttg
     2701 cccatcatcg tggggcgcac ctataaccag gacaccatgc cgccgtgggg gctgccgggc
     2761 atggcgtcgc agagtgggat attcagccac tcattgcagg gagggccgac taacggaaat
     2821 atgctgcgct tcgatgataa aacgggtgcg gaagaggtga agttccatgc ggaaaaagac
     2881 ctcaatacca cggtaaaaaa caatgaaacc cacacggtca tggtcgatcg cataaaaacc
     2941 attgtcaaaa atgaaaccat cagcgtggga gaagaccgta atgccaccat cacaaaaaat
     3001 gatggtcttt ccgtcaaact ggctcaaacc atcaatgtcg gaacgacata tcgtctggat
     3061 gttggcgatc aatttaccct tcgttgcggc aatgcagcgc ttgtattaca caaggatggc
     3121 tcgattgagt tttgcggaaa acaactgtta ctgcacacca gcgatgttat gcaactcatt
     3181 ggcagtggca ttgatatgaa tccggatggc ggcacagcca tgactgcgga tgatattgcc
     3241 ccgttaccta cccacgacgt ttctgaataa aaaatataaa aggaattttt ctatgaccag
     3301 cctttaccgt attcaggagg gctgctttgc cctgcctgaa acttttcttg atcgcaccgt
     3361 caatatcttc gtcccatcgg gcaatgaaag ggcaactccc agtctgaata ttttccgcga
     3421 tacgctcagg ccagatgaaa atctgaccac ctatattgac cgccagatag ccttgatgaa
     3481 aaaaaaaacc taggccagca ccgggtactt tcgcggggaa cggcactggc gggaactggc
     3541 gaagctgcca tgacaggtga acagattctc gcaactcatc gttctggaaa aacagaagtg
     3601 taccagcgtc aggccgggtt tatcaccggg ccagcaaagg tactaatgtt gacgctgacc
     3661 actcagcgtc cttttgatga tcacaccgac cagctatgga ctgcatggtt gactagtttt
     3721 cagcctgcta aaagctaaaa aaaacaggga ggttttgtca tgtatgaagc cgcacgggtg
     3781 gatgacccca tctaccacac cagcgccctt gccgggtttt tgataggggc catcataggt
     3841 atcgccatta ttgccgtcgc cagctttgct ttttttacct gcggcttttt ggccgggctg
     3901 attttgagtt ttctggctga tcagatagct tccggagtcc tgcaactggg tgaggctatt
     3961 gggcgctcga tacattccac agtgggaaaa attctgaccg gttctgataa tgtcagcacc
     4021 aacagtcgcc cggccgcccg ggccgtactg agtacggtga tatgcgaaga ccattccccg
     4081 gaaatccgca ttgcccaagg ttccggcaac atctacatca acagccagct gcccgtaagg
     4141 atgaccacac tgagtgtgat gcagtgattg aggacggctc ccccaatgtc tttctcggcg
     4201 gcggtacgca gacggtactg gaaatcagcc cggaaatccc tgactggctg cgtcaggtgg
     4261 tggatgtgct gtatgtggtc gccggaatgc ttggcgggct ggctggcgcc tggcgtcagg
     4321 cggcaaagat ggggtcgaaa tttggtacaa aatgtgcggc aaaatttatt ggtggcgaaa
     4381 tggtcgggat ggccatctct gacacagtca tgggattgtt cagcaatccg gtagatgtca
     4441 ccactgggca aaaaattttg ctgccggaga cggattttac cctgccagga cgcctgcctg
     4501 tcacctgttc gcgcttttac gccagccacc tggaaactga aggattgctg ggcagaggat
     4561 ggcggctgaa ctgggagata aacctgcgtg aggatgaaac atatatcacc ttcatcggtg
     4621 tacagggtcg ggaactgagt tatccgaaag agatgctgat accgggccac cagatattcg
     4681 acccggaaga gcagttttac ctgagccgtc tgcatgacgg atgctatgtg ctgcattaca
     4741 ccgactgcag ctattacgtg tttgatgaat ttgatgacca cggcgtcgcg ccgttacttt
     4801 ttatggagac gccgtaccgg cagcgtattg ctttcgggcg tgagaacggc aggctggtgc
     4861 gggtggcctc cagcagcgga caccatctgt tattgcaccg cacgatgacg caagcagggg
     4921 aacgcctgtc gcatattgaa cttttgaagg gcgggcgtcc cggtaacctg gtggaatatc
     4981 gttacgacga taacgggcaa ctgactggcg tggtgaaccg ggctggtgtg acggtgcgcc
     5041 agtttgccta tgaaaatggc ctgatgacag agcatcgcaa cgcgacgggc tttacctgta
     5101 cctaccactg ggaagagatt gaaggtttcc cgcgcgtggt ggaacacacg accagcgacg
     5161 gcgaggatta ccggttccac tatgattttg ctggcgggca gacggtggta accggcaggc
     5221 cggagcagaa gtggcagtgg tggtttgatg aagagacgta tgtgactgcg caccggacgc
     5281 cgggcggcgg aatgtaccgc ttcacgtaca acgaaaacca ttttccggta gcggtggaac
     5341 tccccggcga acgcagggta acgctggaat acgacaccct gagccgggtg gtgaaagaga
     5401 cggacccggc aggccgggtg acacagacgc agtggaacgg cagttttgct gaaatcacgc
     5461 gccgggcgct ggatgacgac cacgtatgga aggcggatta caacgaacac ggccaggtga
     5521 tacgggagac ggacccggaa gggcgcgtca cgcggtacgg gtatgatgat caggggctgc
     5581 cggagacggt ctgccacccc ggtaaacagc aggaccgtta cacctggaac gcgctggggt
     5641 tactgagcag ccaccgtcgc atcacgggca gtgtgcagtc ctggcagtat acgcagcgcg
     5701 ggatgctggc gcgccatacg gatgaagaga aacgggagac gcgctggcag tacaccccgg
     5761 aagggctggt tgccagtctg agcaacggca acggggctca gtaccggttc agctacgacg
     5821 gcgacggacg gctgacggga gaacagcgcc cggacggcct gataaggatg tttgcgctga
     5881 atgcggacgg gtttccggtc atcatcccga cgcagggcac agagggcggg gtgcgcaatg
     5941 aacagcagga gcgtgatgcg ctgggccgcc tgctgcgcag tgacacgcag cacagtacac
     6001 ggaccttcag ctacaaccgt cttgaccaga taacggaagt gacgctcacc cccacggaag
     6061 aaggggaacg gctgcaccac atgcaggcgg acacggtgcg ttttgcgtat gaccgcagcg
     6121 ggtggctgac ggcagagcac agcgtgcacg gcagtatcaa ataccggcgc gatgcgctgg
     6181 gcaatcccac cgatatcacg ttgccggacg ggcagcatct cagtcatctt tactacggca
     6241 gcgggcatct gctccagacg gcactggacg gtatcacggt cagtgaatat gagcgtgaca
     6301 gcctgcaccg tcaggtgata cgcacgcagg ggaaactggc gacgttcagc ggctacaacg
     6361 ccgacaaccg gttaagctgg cagcgcagcc tgccaggtgg cagccagaat ccacagcagg
     6421 cggtactccc ccggcgcaga acgacagcgt gacggcgcgg gactatatct ggaacgcaga
     6481 cggtgaagtc ggcggcatca atgacaaact gcgcggttgc ctggtgttca gctatgaccg
     6541 cagcggctgg ctgaccagcc gcacggggca gatgtatgac catgaccatt attattacga
     6601 caaagcgggc aacctgctga cggatgagta tcagggagcg gtgatggata accgcctgcc
     6661 gggttacggg cgtgaccgct accgctataa cgaatggggc gaactgacag agcgcaggga
     6721 ccagcagctg gagtggaacg cgcagggcca gttaacgcgg gtcatcagca gcaacagcga
     6781 gacgcggtat cagtacgatg cgctgggcag gcgcatcagc aaggcgacca gcaatctcca
     6841 taccgaccgg ggagaacgaa gccggacaac gtttgtgtgg gaagggttcc ggctgttaca
     6901 ggagacaacg tggcagggaa agcggacata cctgtacgat gcggaacagc cgtacacgcc
     6961 ggtggctgcc atcacggggc gcggagagag ccagaaaatc tggtattacc acacggacct
     7021 gacgggcacg gtacacgagg taaccgcgcc ggacgggacg ctggtgtggg cgggctacca
     7081 ggcgggattt ggtgaaaacc ggggcgacat cagcaacagc ggggcgtact ttgagcagcc
     7141 gcagcggctg ccggggcagt actttgacga agaaacaggg ctgcattaca atctgttcag
     7201 gtattatgcg ccggagtgcg ggaggttcgt aagccaggac ccgataggat taaacggtgg
     7261 gttgaactta tatgcttatg cgcctaatcc gttaggttgg atcgatccgc taggactgaa
     7321 ttctttagga atagattcaa ataatatatt tagaggagat ggtaactata aaggtggcgg
     7381 aattggacag ccaaagagtg gcacaatatc agcacaggac attattgatc atctctaaga
     7441 aaaagg
//
LOCUS       DAZSNM010000198         7399 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZSNM010000198 DAZSNM010000000
VERSION     DAZSNM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.198"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..199
                     /locus_tag="ACHW42_003459"
     CDS             <1..199
                     /locus_tag="ACHW42_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HFR8945126.1"
                     /translation="ILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGW
                     VIVVLVVALNIWLLVGTALGL"
     gene            complement(340..666)
                     /gene="ypeC"
                     /locus_tag="ACHW42_003460"
     CDS             complement(340..666)
                     /gene="ypeC"
                     /locus_tag="ACHW42_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR8945127.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(781..2037)
                     /locus_tag="ACHW42_003461"
     CDS             complement(781..2037)
                     /locus_tag="ACHW42_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR8945128.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            2241..3206
                     /gene="glk"
                     /locus_tag="ACHW42_003462"
     CDS             2241..3206
                     /gene="glk"
                     /locus_tag="ACHW42_003462"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR8945129.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            3425..3751
                     /locus_tag="ACHW42_003463"
     CDS             3425..3751
                     /locus_tag="ACHW42_003463"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR8945130.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            3773..5020
                     /locus_tag="ACHW42_003464"
     CDS             3773..5020
                     /locus_tag="ACHW42_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFR8945131.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            5035..5637
                     /gene="ypdF"
                     /locus_tag="ACHW42_003465"
                     /pseudo
     CDS             5035..5637
                     /gene="ypdF"
                     /locus_tag="ACHW42_003465"
                     /inference="COORDINATES: protein motif:HMM:NF007310.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            5598..5705
                     /locus_tag="ACHW42_003466"
     CDS             5598..5705
                     /locus_tag="ACHW42_003466"
                     /inference="COORDINATES: protein motif:HMM:NF019360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945132.1"
                     /translation="MARGAAARQSQRQDCCSGRVCHSRFWCAVSGLLLI"
     gene            5702..6118
                     /locus_tag="ACHW42_003467"
     CDS             5702..6118
                     /locus_tag="ACHW42_003467"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFR8945133.1"
                     /translation="MTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQ
                     QIDDAARRVITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIY
                     LLGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            6118..7155
                     /gene="ypdE"
                     /locus_tag="ACHW42_003468"
     CDS             6118..7155
                     /gene="ypdE"
                     /locus_tag="ACHW42_003468"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR8945134.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            7180..>7399
                     /locus_tag="ACHW42_003469"
     CDS             7180..>7399
                     /locus_tag="ACHW42_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFR8945135.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEE"
BASE COUNT         1690 a         1887 c         2050 g         1772 t
ORIGIN      
        1 gattctggtt atgagtcagg tgctgttaag ttttggtatc gccctggcgc tggttccact
       61 gctgattttc accagtgaca gcaagttgat gggcgatctg gtgaacagca aacgcgtaaa
      121 acagacaggc tgggtgattg tagtgctggt cgtggcgctg aatatctggt tgttggtggg
      181 gacggcgctg ggattgtagt tgaatgagcg tcgcatctgg cactattggc ggatgcggcg
      241 taaacgcctt atccgcccta cgcgttaaag agccggtttg taggcctgat aagacgcgct
      301 agcgtcgcat caggcattgt gctccattgg aggacgcgtt taatggcggt gcccatgtcc
      361 gcggcctttt ccgcgatgat cgtcacgatc gcgccagcct tcacgatagc cacgctcata
      421 tgctttacgc ttatcccagc cacgatgata gccattatca tgacgccacc agcggttttt
      481 gcgccattca taattgcgat gccagtaatc acgatctcgc cagtgaccac cgtcccagta
      541 attaccgtaa tgatcgcgat cgccaatttg taatttgatc gatggcagta gggtgatttc
      601 acctgcattt gctgcaagcg gggtaaatgc catcagggcg gccgccagaa acagtgacct
      661 gaacattgtt attctccttc acgatcgaag ccgtcagcgg cctgttaacg caatattacg
      721 gggaggtaaa gccccgtatc atcgccataa ctcttaaatc ataaaggaga gcattttttg
      781 ctaaacatta tcgtggggtg gctgttgctt cggacgattg accatcatca tcggcttacc
      841 tgctaataac agccatgccg gaagcatgac gatacagagc agcggtagca atgtggtatt
      901 gggtacaacg actgccgcca taaaaagact taaccagcca tcgcgtgtta ccaccagcac
      961 gatgccgaga atagcgcagg aaacggttat tgccgctgga acggcgggaa cgtgctcatg
     1021 cagcatcaac cctaatgcca cgccgacaaa cactgccggg aagattcgcc caccgcgaaa
     1081 gccactggcg gcagcaacga ccagggcggc aagtttaatt accgccagca aaaagtaatc
     1141 gctggtgctg aaagcctgat ttgccaccat ctgctgcatc tcatccagcc ctttaaacag
     1201 cgaaactggt ccaccaataa cccccagaat tccgagaata aatccgccaa tacccagcac
     1261 gagcaccgga tttttcattt gattcatcat cgcgtgcaac cgtggtaagc accatacggc
     1321 gaccatccct gctgcgatgg caatcgccgc gacaattgca ccgctgagaa tatcggtcat
     1381 ttccatctgt ccgtaatgcg caatgggcag tgaaaaatgg ggatggaaaa ataatccggt
     1441 agtaagtgca ccagctgctg ccgccattaa cggcgcaaaa agacgatccc atagcggaac
     1501 ttcactactg ccatttaagg tttgcgaaaa tatcaacgcc gccgcaacag gtgtaccaaa
     1561 cagcgcgccg atggttccgg cagaggctaa aatagtccac tccattcggt tgacgcgcgg
     1621 taacagacga gcgccaatgg cgacggcaag ggcgatattg acggtcatga tcggatgttc
     1681 cggccccagg ctgacgccgc cagcaagacc gagaattaat gctacgataa gtctaggtag
     1741 cgcagagggc ggaaccggtg cgccgatcag cggttcacag gcggggtctg gtccggcatg
     1801 accctggctg aaacggataa ccaaccccac cgcaataccc gttagcgtta atacaccgat
     1861 gatccagagg ggtgaatcct gggctatccc cagagttccc ggcagtcgtt gccagagcaa
     1921 attctgtaat accgaggcga ttttcatcac cacaatcaga ataagactgg acgcaatccc
     1981 aattgccacg gcggggagcg ataataacaa catggttctg gctcgcggat ggagcatgtt
     2041 ctattcctta tgcggggtca gatacttagt ttgcccagct tgcaaaaagg catcgctgca
     2101 attggtgctg aaacgataaa gtaattgtgt gacccagatc gatatttaca gggagcctgc
     2161 ctttccggcg ttgttgttat gcccccaggt atttacagtg tgagaaagaa ttattttgac
     2221 tttagcggag cagttgaaga atgacaaagt atgcattagt cggtgatgtg ggcggcacca
     2281 acgcacgtct tgctctgtgt gatattgcca gtggtgaaat ctcgcaggct aagacctatt
     2341 cagggcttga ttaccccagc ctcgaagcgg tcattcgcgt ttatcttgaa gaacataagg
     2401 tcgaggtgaa agacggctgt attgccatcg cttgcccaat taccggtgac tgggtggcaa
     2461 tgaccaacca tacctgggcg ttctcaattg ccgaaatgaa aaagaatctc ggttttagcc
     2521 atctggaaat tattaacgat tttaccgctg tatcgatggc gatcccgatg ctgaaaaaag
     2581 agcatctgat tcagtttggt ggcgcagaac cggtcgaagg taagcctatt gcggtttacg
     2641 gtgccggaac ggggcttggg gttgcgcatc tggtccatgt cgataagcgt tgggtaagct
     2701 tgccgggcga aggcggtcac gttgattttg cgccgaatag tgaagaagag gccattatcc
     2761 tcgaaatatt gcgtgcggaa attggtcatg tttcggcgga gcgcgtgctt tctggccctg
     2821 ggctggtgaa tttgtatcgc gcaattgtga aagctgacaa ccgcctgcca gaaaatctca
     2881 agccaaaaga tattaccgaa cgcgcgctgg ctgacagctg caccgattgc cgccgcgcat
     2941 tgtcgctgtt ttgcgtcatt atgggccgtt ttggcggcaa tctggcgctc aatctcggga
     3001 catttggcgg cgtgtttatt gcgggcggta tcgtgccgcg cttccttgag ttcttcaaag
     3061 cctccggttt ccgtgccgca tttgaagata aagggcgctt taaagaatat gtccatgata
     3121 ttccggtgta tctcatcgtc catgactatc cgggtcttct cggttccggt gcacatttac
     3181 gccagacctt aggtcacatt ctgtaaatcc ttcctgttat atcgggaggt aactctcccg
     3241 ataatctttt aaatcataca gttaattcaa tttttctttg tgtcccctca caaggccgac
     3301 ctgcgtcaca cttccgtaca gcgggattaa ttctccagta aatgcattat ttgtctggta
     3361 acggcgattt gttttgcacg ttcataattt cactagtcaa ctatcactga acgaggaatt
     3421 aacgatgagt aagaaactga ttgccttatg tgcctgccca atgggcctgg ctcacacttt
     3481 tatggccgct caggtgctgg aagaagcggc ggtagaagcc ggttatgaag tgaaaattga
     3541 aacccagggc gcggacggca tccagaatcg cctgacggcg caggatatcg ccgaagcgac
     3601 catcatcatc cattccgtgg cagttacccc ggaagataac gaacgtttcg aatcacgcga
     3661 cgtttatgaa atcactttgc aggacgcaat taaaaacgct gcgggcatca tcaaagaaat
     3721 cgaagagatg attgcctctg aacaacagta atattcgaca gggataattg ttatggccat
     3781 taaaaaacgc agtgcaaccg ttgtgcctgg cgcatccggt gcggcagcgg cagttaagaa
     3841 tccgcaggcc tctaaaagca gcttctgggg tgaactcccg cagcatgtga tgtcagggat
     3901 ttcacgcatg gtgccgacct taattatggg cggtgtgatc ctcgctttca gccagctgat
     3961 tgcttatagc tggcttaaaa ttcctgctga gatcggcatc atggatgctc ttaatagcgg
     4021 gaaattctcc ggtttcgacc tctccttact taaatttgcc tggctgtcgc agtccttcgg
     4081 cggcgtgttg tttggttttg ccattccgat gtttgctgca tttgtggcga actctatcgg
     4141 tggcaaactg gcgttcccgg ctggttttat cggcgggttg atgtctaccc agccgacgca
     4201 actgctgaac ttcgatccca gcactatgca atgggcgacc tcttcgccgg tgccgtccac
     4261 cttcattggt gcgctgatta tttctatcgt tgccggttac cttgtgaaat ggatgaacca
     4321 gaaaatccag ttaccggatt tcctgttagc gttcaaaacc acatttttgc taccgattct
     4381 ttccgccatt tttgtcatgc tggcgatgta ctacgtcatc actccctttg gtggctggat
     4441 caacggcggt atccgtactg tgctgactgc cgcaggtgag aagggcgcgc tgatgtacgc
     4501 aatggggatc gccgcggcga cagcaatcga ccttggtggc ccgatcaaca aagccgcagg
     4561 tttcgttgcc ttcagcttta ccactgacca cgtattaccg gtcaccgctc gttctatcgc
     4621 tatcgttatt ccgccgattg gtctgggtct ggcgaccatt attgaccgtc gtttaaccgg
     4681 caaacgcctg ttcaacgctc aactttatcc gcagggtaag accgcgatgt tcctggcctt
     4741 tatggggatt agtgagggtg cgattccgtt tgcgctggaa agccccatca ccgctattcc
     4801 gtcatatatg gtcggcgcga ttgtcggctc aaccgcagct gtctggctgg gcgcagtgca
     4861 atggttcccg gaatccgcta tctgggcatg gccgctggtc actaacttgg gcgtttatat
     4921 ggcggggatc gcgctggggg caatcattac tgcgctgatg gtggtgttcc tgcgtctgat
     4981 gatgttccgc aaaggcaaat tgttaatcga tagcctgtaa gaaggaaaat ccagatgaca
     5041 ttactcgctt cgctgcgcaa ctggcttaag gtgcaacaac tggatgcggt gcttctctcc
     5101 tcacggcaga acaaacagcc gcatctgggg atctccaccg gatcaggcta tgtggttatt
     5161 agccgtgaaa gtgcgcacat tctggtagat tcgcgctatt tcgttgaggt ggaagcccgt
     5221 gcgcaaggct accagctgca tttgcttgac gcaacgaaca cgcttaccac tatcgtcaat
     5281 caaatcattg ccgatgagca gttgcaaacg ctcggttttg aaggccagca ggtgagttgg
     5341 gaaaccgcgc accgctggaa gtctgaactc aatgcgaaac tggttagcgc cacgccggat
     5401 gtgctgcggc aaatcaaaac gccagaggag gtggagataa tccgcctcgc ctgtgggatt
     5461 gccgatcgcg gagcggagca tattcgccgc tttattcagg cggggatgag cgagcgcgag
     5521 atagccgctg aactggagtg gtttatgcgc cagcagggcg cagaaaaagc ctcttttgac
     5581 accattgtcg ccagcggctg gcgcggggcg ctgccgcacg gcaaagccag cgacaagatt
     5641 gttgcagcgg gcgagtttgt cactctcgat tttggtgcgc tgtatcaggg ctactgctga
     5701 tatgacgcgc accttgctgg tgaatggcga aggggtgagc gccgaatctc acccgctgtt
     5761 tgacgtctat cagattgtcc tgcaggcaca gctcgcggca atctctgcaa ttcgccccgg
     5821 cgtgcgctgc cagcagattg acgacgccgc gcgccgggtc attaccgagg ctggatttgg
     5881 cgactatttc ggtcataaca ctggtcacgc tatcggcatt gaagtccatg aagatccgcg
     5941 tttttcaccg cgggacacca cgacgctaca gccaggcatg ttgctgaccg tggagccggg
     6001 gatttatttg ctagggcaag ggggcgtgcg catcgaagat gttgtgctgg ttaccccgca
     6061 aggcgcagaa gtgctctacg ccatgccgaa aacagtgttg ctcacgggag aggcataatg
     6121 gatttatcgc tattaaaagc gttgagcgag gcagatgcga tcgcctcctc ggaacaggaa
     6181 gtgcggcaga tcctgctgga agaagcggct cgcctgcaaa aagaagtgcg atttgatggt
     6241 ctgggatcgg tgctgatccg cctcaatgaa tcgacaggtc cgaaggtgat gatctgcgcc
     6301 catatggacg aagtgggatt tatggtgcgc agcatctccc gcgaaggcgc tattgacgtg
     6361 ctgccggttg gcaacgtgcg catggctgcc cgccagctgc agccggtgcg catcaccacc
     6421 cgtgaagagt gcaaaattcc aggcctgctt gacggcgacc ggcaggggaa tgacgttagc
     6481 gccatgcgcg tggacattgg cgcgcgcacc tatgacgaag tgatgcaggc gggaattcgt
     6541 ccaggcgatc gcgtcacctt cgataccact tttcaggtcc tccctcacca gcgggtgatg
     6601 ggcaaagcct ttgatgatcg cctcagttgc tatctgctgg tgacgttact acgtgagctg
     6661 cacgacgccg aactgcctgc ggaagtgtgg ctggtggcaa gttccagcga agaggtggga
     6721 ttacgcggcg ggcaaactgc tacccgcgcg gtgtcgccgg acgtcgccat tgtgcttgat
     6781 accgcctgct gggcgaaaaa ctttgattat ggcgcggcta accatcgtca gattggtaac
     6841 gggccgatgc tggtgttaag cgataaatcg ctgattgcgc cgccaaaact taccgcgtgg
     6901 attgaaaccg tggcggcaga aattggcgtt ccgttgcagg cggatatgtt cagtaacggc
     6961 ggcacggacg gtggagcggt gcacttaacc ggtactggcg tacccacatt ggtaatgggg
     7021 cctgccaccc gccacggaca ttgcgcggca tcgattgccg attgccgcga cattttgcag
     7081 atggagcaac ttttatctgc ccttattcaa cgtcttacgc gtgagacggt tgttcaactg
     7141 acggatttca gatgatctcc tgattaaccc ggagcggtta tgttaacgat tcaatttctc
     7201 tgtcctttgc ctaacggtct acatgctcgt ccggcgtggg aacttaaaga acagtgcagc
     7261 cagtggcaaa gcgaaatcac ctttatcaac catcgccaga acgcaaaggc tgatgctaaa
     7321 agctcgctgg cgttgattgg aactggcacc ttatttaatg acagttgcag cctgaacatt
     7381 agcggcagcg atgaagagc
//
LOCUS       DAZSNM010000199         7375 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZSNM010000199 DAZSNM010000000
VERSION     DAZSNM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.199"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1541
                     /locus_tag="ACHW42_003470"
     CDS             <1..1541
                     /locus_tag="ACHW42_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR8945136.1"
                     /translation="PTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVD
                     TYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGV
                     YSNTDAQDGIPLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFT
                     TFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVV
                     DRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKR
                     ILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKG
                     KLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWI
                     TSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVEN
                     NQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKAL
                     SEAK"
     gene            1593..2750
                     /locus_tag="ACHW42_003471"
     CDS             1593..2750
                     /locus_tag="ACHW42_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR8945137.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="ACHW42_003472"
     CDS             complement(2716..2838)
                     /locus_tag="ACHW42_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945138.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3041..4726
                     /locus_tag="ACHW42_003473"
                     /pseudo
     CDS             3041..4726
                     /locus_tag="ACHW42_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4764..7124
                     /locus_tag="ACHW42_003474"
     CDS             4764..7124
                     /locus_tag="ACHW42_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR8945139.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7181..>7375
                     /locus_tag="ACHW42_003475"
     CDS             7181..>7375
                     /locus_tag="ACHW42_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945140.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
BASE COUNT         2163 a         1615 c         1568 g         2029 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     2941 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3001 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3061 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3121 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3181 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3241 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3301 aatatccgca aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3361 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3421 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3481 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3541 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3601 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3661 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3721 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3781 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3841 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     3901 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     3961 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4021 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4081 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4141 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4201 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4261 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4321 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4381 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4441 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4501 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4561 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4621 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4681 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4741 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4801 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4861 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     4921 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     4981 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5041 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5101 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5161 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5221 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5281 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5341 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5401 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5461 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5521 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5581 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5641 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5701 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5761 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5821 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     5881 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     5941 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6001 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6061 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6121 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6181 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6241 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6301 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6361 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6421 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6481 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6541 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6601 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6661 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6721 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6781 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6841 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     6901 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     6961 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7021 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7081 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7141 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7201 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7261 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7321 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattg
//
LOCUS       DAZSNM010000200         7372 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZSNM010000200 DAZSNM010000000
VERSION     DAZSNM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.200"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(466..1662)
                     /gene="hemY"
                     /locus_tag="ACHW42_003476"
     CDS             complement(466..1662)
                     /gene="hemY"
                     /locus_tag="ACHW42_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR8945141.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1665..2876)
                     /gene="hemX"
                     /locus_tag="ACHW42_003477"
     CDS             complement(1665..2876)
                     /gene="hemX"
                     /locus_tag="ACHW42_003477"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR8945142.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(2898..3638)
                     /gene="hemD"
                     /locus_tag="ACHW42_003478"
     CDS             complement(2898..3638)
                     /gene="hemD"
                     /locus_tag="ACHW42_003478"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR8945143.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3635..4576)
                     /gene="hemC"
                     /locus_tag="ACHW42_003479"
     CDS             complement(3635..4576)
                     /gene="hemC"
                     /locus_tag="ACHW42_003479"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR8945144.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4963..>7372
                     /gene="cyaA"
                     /locus_tag="ACHW42_003480"
     CDS             4963..>7372
                     /gene="cyaA"
                     /locus_tag="ACHW42_003480"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR8945145.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINF"
BASE COUNT         1652 a         1942 c         1915 g         1863 t
ORIGIN      
        1 tattgatgct gtttttagtt ttaacggcaa ttaatatatg tgttattaat tgaatgaatt
       61 ttatcattca taataagtat gtgtaggatc aagctcaggt taaatattca ctcaggaagt
      121 tattactcag gaagcaaaga ggattacaga attatctcat aacaagtgtt aagggatgtt
      181 atttcccgat tctctgtggc ataataaacg agtagatgct cattccatct cttatgttcg
      241 ccttagtgcc tcataaactc cggaatgacg cagagccgtt tacggtgctt atcgtccact
      301 gacagatgtc gcttatgcct catcagacac catggacaca acgttgagtg aagcacccac
      361 ttgttgtcat acagacctgt tttaacgcct gctccgtaat aagagcaggc gtttttttat
      421 gcatcagaaa ggtcccggag gtgctttcct ccgggtgaga aggaactact gtggcgggtt
      481 attctgcaac gttaacatca aaccgtcgcg acgcatcgct gcagcttctt ccggcttgtg
      541 cagtctgtcc agcgcgtcgg caagccatgc gtaatcgtag gcgtccggac gttgtttcag
      601 cgccgcgcgg aaggcgagcg atgcttcctg ccattcaccg tgcttcatca gtgactgacc
      661 caatgtgctc cacaacagcg ggcgatcacc gacgtttttg atttgctggc gtagcacttt
      721 ttccagctgt tccgggttat tggttttcag acgcgggatc ggcagcagta ggcgatcgtc
      781 atactggcgt ttcaggccgt cgatgataat ttgctgggca gtatcatgat cgtcacattc
      841 aataagatgt tccgccattg ccacctgcaa cgctacctga tgacgcgttt tccggctttg
      901 gtttttccac cagttacgca aaccttcact accgttatcg gcacgcgcct gatccatcag
      961 gccaatccat gcctgttgtt ccagcattgc acgatgttct tcatcaccaa catgggcttt
     1021 cgccattgat gggatgatat ccagcagaga actccatgcc cctgtgcgga tatacgcctg
     1081 ttccgccaga cgtaatactt caggatggcg tggcgtaact tccagcagct tatccacgcc
     1141 gtggcgtgca gcatggtttt cattacgggc cagttgcaga cgtacacggg tgatttctac
     1201 cggaatggtg tcattgccgg ccagctccgc tgcgcgttcc agatgttggt tggcgcgtgc
     1261 ttcatcacca cgttgttgcg cggcttcggc agccagtaga tagttcacca ccggttgttc
     1321 agcgtgatcg gcatttttcg ccatcagctt ttcaacttgc tgataatcac cttccgccag
     1381 tttcagcagc gcctgttcgg tctgcttacg cgcacggcga cgcttacgtc cgacaaacca
     1441 tccacgggtg tgcgcgccag tgcggaagat ccgccgcagt agccactcaa tggcaaacag
     1501 cactaccatc gccagaatca atatgatcgc caggcccgtg acgctggttt cgatattgta
     1561 gttgtcggtc tggatcagca cataaccctg atgaccggca atcatcgggc caaccactat
     1621 ccccgcaatc agcaaaacaa agagtaataa cacttttagc atggttattc tccttgcggc
     1681 gcggctgccg gagtatcacc ttgcggtgca ggtgcaggtg caggtgcagg tgcaggtgca
     1741 ggtgcaggtt tagcttccgt tgtccccgct gccggttgtg ccagcaggtt acgcacgcga
     1801 gtctgcatca gtttttccag catcgcctgg ctttgcaggg tttccggaag atccatcgag
     1861 atattttgct ggcttaactg gtccacctcg tcgaggaacg ctttggtggt ggcatcatca
     1921 gtatcgtagt aagcacgtac ccaggtggag acgttctcca gcgcctggcg ataagtctct
     1981 tcctgatgac gcggtacagc ttgtgctgcg accagcaggc gagagcgaat attttcgcgc
     2041 agatagatat cctgatttgg cgctaacagc ggtacggcgg tgtcatcacg acggcgaatc
     2101 gtaatgaagt tgtccataaa gttctgccag cttttttgca gattgatacg ccattcgctg
     2161 atggaactgg aaagctcttc accgtctgaa tccatcggcg aaccatcgct gtcattatcg
     2221 gccagacgca ggttatctac ctgatttgaa agctgattaa gcttaaggat gatgccgtca
     2281 taatccacct gcgatactgc agaaaggctg gcgatatcat cggtaattgc ccgacgaacg
     2341 gtaatcagac tcgggtcatt catatccgcc aggctggcgt ctgcactttt cagcaacgct
     2401 gcagaggtcg tgacgtcctg atcgctccac agcttccgtc cggcgagttt caccagaaaa
     2461 tcggcctgag ccagcagcca ggttttagca tcgctgccgg aaatggtggc gatcttttgt
     2521 tggacttcat ccaactgttt tgccagcgtt tcttgctgac gattcgcctg cttaagctgc
     2581 gcagcttgtt gcttaataat gccttccagc tcggcttttt ggctctcctg ggctttttgc
     2641 aatgccgtca gttggttagc cagggcatcg ctggtggcgg tctgattgac ggcctgttgt
     2701 ttaccccagc catacaaacc gacacccgcc gccagagcaa tagcgatagc caccgcgctg
     2761 agaatcaatg cagtattgtt cttactcttt ttttctgttg cgacaggttg tgacgtggtg
     2821 tccacggcct ccctggtctc ttcaaccacg gcggaggttt tttcttgttc cgtcattatg
     2881 gcttcctgtt atgagagtta ttgtaatgcc cgtaaaagcg catcgttgtc agcgttatcg
     2941 gcgaccttaa tgtcttgcca gcccagttcc cgggcgagtt tcgccaaacg ctcactgacg
     3001 accaatagtc gacagtgtag taaccagtgc tcacgatacc attgtgggat cagcgaccag
     3061 agttgctgca acatttcacc gctggtaaca acgaccatcg tcacctcgcg ggattgccag
     3121 cgcatcgctt cttctgcacc atcgtaatgg attgcgcatc gttgataaca ttcacaaaaa
     3181 gtgacctcag caccgcgcac cgtcagggta tccccaatta gctcacgacc gccattgcca
     3241 cgtaatatca gcgcacgttt gcccgcaata ttttgtaatt caggtaattg tagcaagact
     3301 tcgctgattt cccgatcctg tgggtagaga atcttctgtc cgcttacggt atgtagcgcc
     3361 agtgcggtgg tgcgtccaat ggcgaaataa gtaggtagtt ggggccattt acgatcttgc
     3421 tgatgcagct gtgattgggc aaaagcaacc gcgtgttgcg agagggcaaa caacagatcg
     3481 ctctccccca gcgctgccag ttgatcagca agttgcggta attgtcgacc cggagaaaac
     3541 tcaatcagcg gaaaatgcca ggccacctgc cccagtgtgc gcagacggct cactaactct
     3601 tctccagcgg gagacgggcg ggtgaccagg atactcatgc cggggcctct ccgttataga
     3661 cttcagcgag gatctcgcgc gcgccgttat tcagtagctc ttctgccagc gaaatcccca
     3721 tttgttcggc atcttgcggc gcaccgcggc gttcaccgcg aataatctgc gaaccgtccg
     3781 gcgcgccgac cagcgcacat agccagattt cgccatcaat aagctcggcg tagctaccaa
     3841 ttggcacctg acatccgcct tcgagacggg tattcatggc gcgttctgcg gtaacgcgca
     3901 gtgcagtttc gtggtgattc agcgcggcaa gcagctcgcg agtgcgtgta tcatcaaggc
     3961 ggcattcaat accgactgca ccttgtccta ccgccggaag agaaatctcg ggtggtaacg
     4021 cggcgcgaat gcgggactcc agacctaaac gttttagtcc ggctacggca agaatgatgg
     4081 catcgtattc gccgttatct agtttgctca ggcgagtgcc gacgttgccg cgcagggagc
     4141 ggataatcag atccgggcgg cgttcagcca gttggcactg gcgacgtaaa ctggacgtcc
     4201 cgacgatact gcctgctggt aacgcatcca gactgtcata gttattggac acaaaggcat
     4261 cgcgaggatc ttcacgctcg caaatagtga ccagccccag accttgcggg aattcaaccg
     4321 gcacatcttt cattgaatgt acggcgatat cagcgcgatt ctccaggagc gcgacttcca
     4381 gctcttttac aaataagcct tttccgccta ctttcgccag cggcgtatca agaatcacat
     4441 cgccgcgcgt caccatcggt accagttcaa cgaccaggcc cggatggctc gccatcaact
     4501 tgtctttgac atagtgtgcc tgccagagtg caagtgggct ttggcgtgtg gcaattctta
     4561 aaacattgtc taacatgctt gttaccgtca ttatcatccg tggtccatcc taacatcctt
     4621 gccagagtga tgtcagtgtt gtggtgaaac gtagacgcct gcgcaaaccg taaaatgagg
     4681 tctggcagtg gatcctgaca ggcgtttcac gccgtcgtaa taaggaattt acagagaata
     4741 aacggtgcta cacttgtatg tagcgcatct ttctttacgg tcaatcagca aggtgttaaa
     4801 ttgatcacgt tttagaccat tttttcgtcg tgaaactaaa aaaaccaggc gcgaaaagtg
     4861 gtaacggtta cctttgacat acgaaatatc ccgaatgccg cgtgttaccg ttgatgttgg
     4921 cggaatcaca gtcatgacgg gtagcaaatc aggcgatacg tcttgtacct ctatattgag
     4981 actctgaaac agagactgga tgccataaat caattgcgtg tggatcgcgc gcttgctgct
     5041 atggggcctg cattccaaca ggtctacagt ctactgccga cattgttgca ctatcaccat
     5101 ccgcgaatgc cgggttacct tgatggtaac gttcccaaag gcatttgcct ttacacgcct
     5161 gatgaaactc aacgccacta cctgaacgag cttgaactgt atcgtggaat gtcagtacag
     5221 gatccaccga aaggtgagct tccaattact ggtgtataca ccatgggcag cacctcgtcc
     5281 gtagggcaaa gttgttcctc tgacctggat atctgggtct gtcatcaatc ctggctcgat
     5341 agcgaagagc gccagttact acaacgtaaa tgcagcctgc tggaaagctg ggccgcctcg
     5401 ctgggtgtgg aagtcagctt cttcctgatt gatgaaaacc gcttccgtca taatgaaagc
     5461 ggcagcctgg ggggcgaaga ttgtggctcc acccagcata tactgctgct tgacgaattt
     5521 tatcgtactg ccgtgcgtct cgccggtaag cgtattctgt ggaatatggt gccgtgcgac
     5581 gaagaagagc attacgacga ctatgtgatg acgctttacg cgcagggcgt gctgacgcca
     5641 aatgaatggc tggatctcgg tggcttaagc tcgctttctg ctgaagagta ctttggtgcc
     5701 agcctttggc agctctacaa gagtatcgat tccccataca aagcggtgct gaaaacactg
     5761 ctgctggaag cctattcctg ggaatacccg aacccacgtc tgctggcgaa agatatcaaa
     5821 cagcgtttgc acgacggcga gattgtatcg tttggcctcg atccatactg catgatgctg
     5881 gagcgtgtta ctgaatacct gacggcgatt gaagatttca cccgtctgga tttagtacgt
     5941 cgctgcttct atttaaaagt gtgcgaaaaa ctcagccgtg aacgcgcctg tgtaggctgg
     6001 cgtcgcgcag tgttgagcca gttagtgagc gagtggggtt gggacgaagc tcgtctggca
     6061 atgctcgata accgtgctaa ctggaagatt gatcaggtgc gtgaggcgca caacgagctg
     6121 ctcgacgcga tgatgcagag ctaccgtaat ctgatccgct ttgcgcgtcg caataacctt
     6181 agcgtctccg ccagtccgca ggatatcggc gtactgacgc gtaaactgta tgccgcgttt
     6241 gaagcattgc caggtaaagt gacgctggta aacccgcaga tttcacccga tctctcggaa
     6301 ccgaatctga cctttattta tgtgccgccg ggccgggcta accgttcagg ttggtatctg
     6361 tataaccgcg cgccaaatat tgagtcgatc atcagccatc agccgctgga atataaccgt
     6421 tacctgaata aactggtggc gtgggcatgg tttaacggcc tgctgacctc gcgcacccgt
     6481 ttgtatatta aaggtaacgg cattgtcgat ttgcctaagt tgcaggagat ggtcgccgac
     6541 gtgtcgcacc atttcccgct gcgcttacct gcaccgacac cgaaggcgct ctacagcccg
     6601 tgtgagattc gccatctggc gattatcgtt aacctggaat atgacccgac agctgcgttc
     6661 cgcaatcagg tggtgcattt tgatttccgt aagctggatg tcttcagctt tggcgagaat
     6721 caaaattgcc tggtaggtag cgttgacctg ctgtaccgca actcgtggaa cgaagtgcgt
     6781 acgctgcact tcaacggcga gcaatcgatg atcgaagccc tgaaaactat tctcggcaaa
     6841 atgcatcagg acgccgcacc gccagatagc gtggaagtct tctgttatag ccagcatctg
     6901 cgcggcttaa tccgtactcg cgtgcagcaa ctggtttctg agtgtattga actgcgcctt
     6961 tccagcaccc gccaggaaac cgggcgtttc aaggcgctgc gcgtttctgg tcaaacctgg
     7021 ggcttgttct tcgaacgcct gaatgtatcg gtacagaagc tggagaacgc catcgagttt
     7081 tatggcgcta tttcgcataa caaactgcac ggcttgtcag tgcaggttga aaccaatcac
     7141 gtcaaattac cggcggtggt ggacggcttt gccagcgaag ggatcatcca gttctttttc
     7201 gaagaaacgc aagacgagaa tggctttaat atctacattc tcgacgaaag caaccgggtt
     7261 gaggtgtatc accactgcga aggcagcaaa gaggagctgg tacgtgacgt cagtcgcttc
     7321 tactcgtcat cgcacgaccg cttcacctac ggctcaagct tcatcaactt ca
//
LOCUS       DAZSNM010000201         7323 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZSNM010000201 DAZSNM010000000
VERSION     DAZSNM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.201"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..78
                     /locus_tag="ACHW42_003481"
     CDS             <1..78
                     /locus_tag="ACHW42_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945146.1"
                     /translation="FDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(160..900)
                     /gene="pflA"
                     /locus_tag="ACHW42_003482"
     CDS             complement(160..900)
                     /gene="pflA"
                     /locus_tag="ACHW42_003482"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR8945147.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="ACHW42_003483"
     CDS             complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="ACHW42_003483"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR8945148.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3429..4286)
                     /gene="focA"
                     /locus_tag="ACHW42_003484"
     CDS             complement(3429..4286)
                     /gene="focA"
                     /locus_tag="ACHW42_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR8945149.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4693..6453)
                     /gene="ycaO"
                     /locus_tag="ACHW42_003485"
     CDS             complement(4693..6453)
                     /gene="ycaO"
                     /locus_tag="ACHW42_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR8945150.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6583..7209
                     /locus_tag="ACHW42_003486"
                     /pseudo
     CDS             6583..7209
                     /locus_tag="ACHW42_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
BASE COUNT         1888 a         1762 c         1794 g         1879 t
ORIGIN      
        1 ttcgatgggg aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat
       61 atggtagatg cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa
      121 aaagcggcaa ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc
      181 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
      241 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
      301 gagaagttcg attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc
      361 cgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt
      421 tttgttcgcc agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt
      481 ttggtggatc tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag
      541 cagttcatca atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg
      601 aatgccttct tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc
      661 ttcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac
      721 cacttccttc atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc
      781 gcggttatga caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat
      841 acctgggccg tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat
      901 tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta
      961 cgtcgagtct gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt
     1021 gctaaaaaag gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca
     1081 gtcaaatcta attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct
     1141 ttagtcagcg agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat
     1201 ttttccgggt tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg
     1261 tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg
     1321 ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag
     1381 gagataccat ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca
     1441 gcacctttct ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca
     1501 cgacgaccgt ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc
     1561 agaacagact gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg
     1621 aaacgttcta ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc
     1681 gggtattcgc cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta
     1741 actttcgcat atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca
     1801 cacgccatgg tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg
     1861 tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag
     1921 tccatgaagt gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc
     1981 ggttcagact tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg
     2041 tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg
     2101 atcatcgggc ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc
     2161 aggtcatcgt tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc
     2221 ttgaagttca gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc
     2281 cccatagtgt acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca
     2341 tcgaggccca taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca
     2401 tcgtattccg gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc
     2461 atttcctgcg cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca
     2521 tccaggaagg tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca
     2581 gccaggtagc cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag
     2641 atgtcgtagc cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca
     2701 gcgatttctt cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc
     2761 tgtagagagg tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca
     2821 acgcgacggt agtcaccgat gatacggcca cggccataag catctggcag accggtcaga
     2881 acaccggatt tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg
     2941 tgagttttac ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac
     3001 gctttgcagg aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc
     3061 ggagcttcag tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg
     3121 tgagaggtga tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt
     3181 tccagtttaa caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca
     3241 gccaggaagg actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg
     3301 tttacttcat tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca
     3361 ttaagctcgg acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc
     3421 gacaaccatt aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc
     3481 aacaaaccac cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag
     3541 ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc
     3601 ggagatgcga agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg
     3661 tgctcaaaac cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc
     3721 atcaggctgc ggccagaata actcatccat actgccagac ataccatcag gtttgccagg
     3781 ataccaagac agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg
     3841 acgtttagtc cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt
     3901 acaaacagca gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac
     3961 tgacaccagg tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag
     4021 agatcggctc cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc
     4081 agttttgcca tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg
     4141 aatgcgattg agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt
     4201 ttcgttgctt tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa
     4261 agatcaaaag ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat
     4321 actaacaaag cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc
     4381 ccgtaactcg catggttttt atgcaaatac ggagtaacta tttgattatc caaaataaaa
     4441 ataaatttta aaaattaaca aatgagttga attttttccg catcctccgc taaaacagtt
     4501 aattaaaagg gagcatcagg cgaataaagt aacaatatcg atcgtattta ttaaatacaa
     4561 attaccgata tttaacctta taattacaat tattttatta atgcaaatat atgtaaagcg
     4621 gggcattaaa aaaacgcccc gtaatataac tcagactaat cattaagcct acattgcgta
     4681 ggctatttga ttttattttg cccagaatgc tgctttggcg cgctgcagct tttcgtaggc
     4741 cttcaacaac gactgatgtg cagcaaacgc gtgcagatcg ctatctaccg gttgcaggcc
     4801 gtaaaacgcc gcttcgccgc tcattgccgc actggcggct tctacggcat ctgcgccgta
     4861 catgcgaaca aacgcattca gatattgcag cggctggcga tcttcttcct gtgccagtaa
     4921 taacaacgtt tgcaggcagc gataatagtt ggcgcgttcc ggactaaata ctgatgagtt
     4981 aaactccatc gtccattcgg tccagaccag agcctgttcc agatcgccac cagccagcgc
     5041 cagcatggct tttaattcac cgatacgcag ggtgtaccaa ccgttatccg acccggtcgc
     5101 cagacccaac agctcacgca cgcgggtaaa gtcatcaaaa ccttcttcat ccagttgctc
     5161 gatgaggttc aggtaatctt ctttttccca ctcgctgcct ggtagcgaaa gaatcgtttc
     5221 acgtaaatgg ctgcccatac tgttattcgc gagccacaga tcttcagccg gataaatatc
     5281 ggacatgcca ggcacgataa tacggcaagc ataaacgccc agatgctcgt aatcggcaat
     5341 ataaacttct ttatcttctt tgttgaagat agccatcagc gtagcaaact cttcttccgt
     5401 ggtgccggag aaattccagt ccacaaacgg ataatcggca tcctgcttga acaggtccca
     5461 ggagattaaa ccgctggaat cgataaagtg cgtttcgagg ttggtatgtt cagcgacttc
     5521 ttcatcatcg aaggttggcg gagtaaacac atccaaatct ttcaggccac gaccttgcag
     5581 cagctcggtc acggtacgtt ccagtgctac gccaaaatca ggatgcgcac cgaaagaggc
     5641 aaagcaggtg ccgtttgcag gattaaacag taccacgcaa atcaccggat actggccgcc
     5701 aagggaacca tcatatgcga agatcgggaa accttctgtt tccagtgttt cgatcgcttc
     5761 aaccaccgct gggtaacgcg ccagcacgtc tgccgggatt tccggcaagc tgatgctttc
     5821 agcaataatg cggtttttca cgtagcgttc gaaaacttcg gacaacccct gaacgcgtgc
     5881 ttcgttgcgg gtattgccag cggacatacc gttagaaacg tacaggttac caatgatatt
     5941 catcggaata taaacggtct gattgtcgga ctggcgcgta aacggcagac cgcaaatacc
     6001 acgatcttcg ttaccggatt gtaggtcaat cagcatactg ccggtcaatt cattctccgg
     6061 atcgtaaaat gcgcgcagac ggtcatcgag cagcccttct ggcacatcgt cattttcgct
     6121 cagtgggaac catttttcgt tgggataatg cacaaatgga ccgttggcga tggtttcgcc
     6181 cagccagaaa tcagcaaaaa agtagttggt tgagagacgc tcgaaatatt caccgagtgc
     6241 agaagccagc gccgctttct tggttgcgcc tttaccgttg gtaaaacaca gtgcgcactc
     6301 tttgtcgcga atatgtacag accagacgtt aggcacggga ttcagccagg aggcctcttc
     6361 aatctgaaag ccgaggtctg aaagtttttg ctggaagcga gcgatggaat cttccagagc
     6421 ggcatctttg ccggggataa atgtttgcgt catgaaaatc actttagtcg tacggaaagc
     6481 gcgcaataat acgggtttta tctcaaaggc gctatcaccg ccgccatgcc gatgaactgt
     6541 tgactatgct tttagcggat aaaccacgtt aagagcataa aaatgaaggc gttcgatctc
     6601 caccgtatgg catttgataa agtgcctttt gatttccttg gcgaagttgc actacgtagt
     6661 ctttatacct ttgtactggt tttttgttcc tcaaaatgac cggaagacgc ggtgtgcgac
     6721 agatgtcgct gtttgaagtg ttaatcattc tcacgctggg ttcagcggcg ggagatgtgg
     6781 cgttttatga tgatgtgccg atggtgccgg tacttatcgt ctttatcact ctggcgttgt
     6841 tataccgcct ggtaatgtga tggcgagctg gcctggtcga aactcaataa ctccaacatg
     6901 acggaatttg agttctttat ggagctacga ttgcgtggcg tggagcagct ggggcaggta
     6961 cgtctggcga ttctcgaaac caacgggcaa atcagtgtct atttctttga agatgacaag
     7021 gtgaaaccgg gtttacttat tttacccagt gattgtacgc agcgttacaa agtggtgccg
     7081 gagtcggcgg actatgcctg cattcgttgt agtgaaatca ttcatatgaa cgcgggggaa
     7141 aaacaattat gtccgcgctg tgcaaatcca gaatggacga aggcaagtcg ggcaaaacgg
     7201 gtgacctgac agtaaaaaca tcggcttttt gctaataatc cgagagattc ttttgtgtga
     7261 tgcaagccac atttttgccc tcaacggttt tactcattgc gatgtgtgtc actgaatgat
     7321 aaa
//
LOCUS       DAZSNM010000202         7315 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZSNM010000202 DAZSNM010000000
VERSION     DAZSNM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.202"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..460)
                     /gene="folK"
                     /locus_tag="ACHW42_003487"
     CDS             complement(<1..460)
                     /gene="folK"
                     /locus_tag="ACHW42_003487"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR8945151.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAF"
     gene            complement(457..1854)
                     /gene="pcnB"
                     /locus_tag="ACHW42_003488"
     CDS             complement(457..1854)
                     /gene="pcnB"
                     /locus_tag="ACHW42_003488"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR8945152.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTT"
     gene            complement(1914..2810)
                     /gene="gluQRS"
                     /locus_tag="ACHW42_003489"
     CDS             complement(1914..2810)
                     /gene="gluQRS"
                     /locus_tag="ACHW42_003489"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR8945153.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWMLTAVPESAIVNSTFSNASC"
     gene            complement(2877..3332)
                     /gene="dksA"
                     /locus_tag="ACHW42_003490"
     CDS             complement(2877..3332)
                     /gene="dksA"
                     /locus_tag="ACHW42_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR8945154.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3510..4214)
                     /gene="sfsA"
                     /locus_tag="ACHW42_003491"
     CDS             complement(3510..4214)
                     /gene="sfsA"
                     /locus_tag="ACHW42_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR8945155.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4229..4759)
                     /gene="thpR"
                     /locus_tag="ACHW42_003492"
     CDS             complement(4229..4759)
                     /gene="thpR"
                     /locus_tag="ACHW42_003492"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR8945156.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4833..7262
                     /gene="hrpB"
                     /locus_tag="ACHW42_003493"
     CDS             4833..7262
                     /gene="hrpB"
                     /locus_tag="ACHW42_003493"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR8945157.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1651 a         1908 c         2072 g         1684 t
ORIGIN      
        1 caaatgctct tgtatgtaag acttcacgca acgtctcccc atcaggaaac gccagctccg
       61 gtgcaatctc aaacagcggc cacagcataa atccacgatt cttcatatcg tagtgcggaa
      121 cggtcaggcg ttcggtattt atcacttcat taccaaacag catgatatcg agatccagcg
      181 tgcgtggtcc ccagcgttca gctttacgaa cacgaccctg ttgcagttct atgcgctgtg
      241 tatggttgag taactcttcc ggggcgggcg aagtttcaag cgccacggcg gcgttgaggt
      301 aatcgggttg atcttgcggc cccagcggtg gggtgcggta aaacgaagaa acggcaagaa
      361 tgcggctttc agggatatcg cctaatgctt tcagggcagc attgacctgc tccagcggag
      421 aggccagatt gctgcctatg gcaatatacg ccactgtcat gtggtaccct cacgacgcgg
      481 tgcgcgtttg cgtggacgac gagtacgacg acgcggtgac ggttcttcat ccagctcgtt
      541 aagcatccct ttttgatctg gtggtgcgga aacctggaac tcaccccacc atttcaccag
      601 acgctgcagt tcagcattac gttcaacttc agctcgcaag gccaacaggt cataagccgc
      661 acggaactta ggatgctcca gcagtttcca tgcgcgttta ccctgacgac gggacatacg
      721 caactgcaac tgccagatat cgcgggttaa cgtcgtcaga cgtttcggga ttgccagtga
      781 acggcaggct tcgtccagca cgtcgttcat cgccagcgcg aaagcgtcgt gataggtcag
      841 gccgctttcc tgggcgatct tctgtgccgt ctccagcagt gggtaccaga acatggcggc
      901 aaacaagaac gccgggttca cgcgcatatc gttatggata cgcgtatcgg tattcttcag
      961 cacctgttca atgatccgct ccatcgggct gtcgccattt tccgtgaagt agcgggtaat
     1021 ggtcgggaac agcggctgga acagatgata ttcacacaac agcttatagg tttcgtaacc
     1081 gtagcccgct tgtagcagtt taagcgattc ttcaaacagg cgtgccggtg ggatatcgtt
     1141 cagcagggta gcgaggcgag ggatcggttc tgcggtttcc gggctgatgc gcatacccaa
     1201 tttggcggca aaacgtaccg cgcgcagcat acgtaccgga tcttcacggt agcgcgtttc
     1261 cgggttacca atcagacgga taacgccgtc cttcagatcc ttcataccac caacgtaatc
     1321 acggacggta aaatccgcta cgctgtaata caggctgttg atagtgaaat cgcggcgctg
     1381 ggcgtcttct tcgatggagc cgaaaatgtt gtcgcgcagc aacatgccgt tttgcccgcg
     1441 ttgggaggtc gtgcggtcgc tgacgttacc ttcgtggtgt ccacggaagg tcgcaacttc
     1501 gataatctcc gggccaaaca ttacatgagc cagacggaaa cggcgaccca ccaggcggca
     1561 gttacggaac agtttgcgca cctgctcagg cgtggcgtta gtggttacgt caaaatcttt
     1621 cggctttttg ccaagtaaca ggtcgcgcac gccgccgcca accagccagg cttcgtatcc
     1681 cgctttgttg agcctgtaca ttaccttcag ggcattttca ctgatatctt tgcgggaaat
     1741 agcatgctgc tcacgcggga tcaccgtcac ctgtggacgg gcgactgcct gttcagcctc
     1801 gctttcctcg cggcttagca ccttgcggca aaaattagcg actcgggtaa aaatagtaca
     1861 cctcggtagt gtcaaacatc attcaggaca aaaaaatagc ggctaatcat agctcagcat
     1921 gacgcatttg agaatgttga atttacaatt gccgactcgg gcacggcggt cagcatccag
     1981 tttttgacgg ctgactgaag gatttgctcg acgctgaaat cctgccagtg tgcttctgcc
     2041 tgctgcccca gaaattgaag tgccgcgatt agtaccgggc gtggatcgcc tttcggcaac
     2101 gcaggcgcat gattctgctt ggaaagttta gcgccttgtg gattaagcgc cagcggcaga
     2161 tgaatgtaat ctggcacttt ccagccaaaa agctggtaca gcgggatttg ccttactgtt
     2221 ggttcaatca gatcagcccc acgcactatt tctgtaacgc cctggaaatg atcatcaacc
     2281 acaacagcca ggttgtaggc gaacaaccca tcacggcgat gaatgataaa atcttcccgt
     2341 gccagttttt cgtcggcgtg aataatgcct cgcagcaggt cagtaaattg cgtgaccgga
     2401 tgctgctggc ggatacgcac tgcggcgttg tctggtccat gatgcaacac ccggcaatga
     2461 ccgtcgtaaa taccgccaat gctttgaata cgcgcacgcg tacaggtgca gtaataactt
     2521 agtccttgtt catgtaacca ggcgagtgct tcacgatagg cgtcgtgacg ttgcgattgc
     2581 cagagaacat cgccgtccca gtgcagaccg taatgttcca gctggcgcag gatagtttct
     2641 gcggcaccgg gaacttcacg aggcgggtcg atatcttcta tgcgtaccag ccagcgacct
     2701 tgccgggcgc gagcctgcaa atagctgccg agcgcggcga tcagagagcc aaaatgaagc
     2761 tcgccggaag gagagggggc gaagcggcca atatactgtg tgtctgtcat ctctttgaac
     2821 aaaaaataag gcgggagcat ttcccgcctg tggtaaacgt gatggaacgg ctgtaattag
     2881 ccagccatct gtttttcgcg aatttcagcc agcgttttgc agtcgatgca cagatcggct
     2941 gtcgggcgcg cttccagacg gcgaatacca atttcaacac cgcaggattc gcagtagccg
     3001 aaatcttcgt cttccacttt tttcagcgtc ttctcgatct ttttgatcag cttacgctcg
     3061 cgatcgcggt tacgcagttc gaggctgaac tcttcttcct gggctgcacg gtctaccggg
     3121 tccgggaagt tggctgcttc atcctgcata tgtgtaacgg tgcgatcgac ttcatccctg
     3181 agttgattac gccatgcttc cagaatacga cggaagtgcg ccagctgggc ttcattcata
     3241 tactcttcgc ccggcttctc ctgatatggt tccaccccag cgatggcgag aatactcagg
     3301 gacgatgttt tacggttttg cccttcttgc atgttgcttc tccttaacac gcactatcga
     3361 tccccatgtt cgggggaaaa atgaggccgc tataaatagc agatgctttt ccggatagca
     3421 attatctaaa cgtaacactt gacaactgtg tgaggaaaag cgtatttgcg cacgcgacca
     3481 gaatgtaaat taaccagtta cttactttac tacaatgtaa ccggcagtga ttttttaaga
     3541 gccatgcctt cagcagaaat ttccgctttg taagccagaa tttctacccc cctctgttga
     3601 gcttctgaca atagttgcgc gtatttctca tcgatgtggc gcgcgggtga aaaccgtgta
     3661 atggctgaat gcagcacggc gaaaaaaata accgcacgct ggccttcagc cgctacgctc
     3721 atcaactccc gaaggtgttt ctgacctcgt tcagtgaccg catcgggaaa atatccctgt
     3781 tcgttctccg ctaacgtaac cgatttcact tcaatatagc agtctggacg cgaatccgcc
     3841 tgcaacataa agtcaatacg gctgcgttcg gagccgtatt ttacttcgct tttcagcgag
     3901 ctatagcctg acagttctga aattgattca ttaaggatag cctctttcgt caacctgtta
     3961 gcccaaagcg tattgacgca aataaatgcg ccgctctggc tttgagttaa ttcccaggtg
     4021 tgtgggtatt tccgtttggt gttgtctgaa gtcgaatacc agacggtatc gccaggcgtt
     4081 gcacaaccgg tcatcgcacc cgtattcggg cagtgtagcg ttaattcgcg accatcgggt
     4141 gtgatcacat cggctaaaaa acgtttgtaa cgctgaatta gcgtcgcgcg ctgtagaggg
     4201 ggagaaaatt ccattgcgac aatccttgtt attgcgttag cgcccagcgt tttagcggcg
     4261 tgtagcgtgt gcgtccacgg gcaaacgagg aggcgtaaag ggtgaactcc gtcaccgcat
     4321 aagaccagtt aaaacccggc ggcgggaatg tcaccgcctc gctggcgtcg cgcaataagg
     4381 taatatgtgg atgaaacgga cgattgcttt gaaaacaacc gctgcgggca gcctgtgaac
     4441 ggagcatatt cgccagctgg attaagccgc gtggaggctg acgcatccct aaccacacca
     4501 cacgcgaacg cagccattgt ccggcgtcat caagcgtgag tgtgaaacca ggttgacgaa
     4561 tccgtccggc taaaagagaa agcgccttct ctttctctgc gctcacttcg cctaaaaatg
     4621 ccagagtcag gtgcaaatta tcggcggcga ccggacgtcc cgcctcaggt gggaagtgtg
     4681 tggcgcgcca atggataatc tgttcgcgga tttctgcagg taagtcgata gcaaagaaca
     4741 gacgttgcgg ttcagacatg tgaggcactc ggttatgaat taccgcgatg ctacaatgtg
     4801 gcgcgaagaa tgttaaccct ctggagcgtt ttgtgtcgtc gttgcccgtt gctgccgtct
     4861 tacctgaatt acttaccgct ctcgattgtg cgccgcaggt attattaagt gcgccgaccg
     4921 gggccgggaa atcaacctgg ctgccgctgc aactgctggc gcatcccggc attaacggga
     4981 aaattatcct gctggagccg cgtcgtctgg cggcgcgtaa cgtcgcgcaa cggctggcgg
     5041 agctgcttaa cgaaaagcca ggcgataccg ttggctaccg gatgcgtgcg caaaactgcg
     5101 tcgggctgaa tacccgcctg gaagtggtta ccgaaggcgt gctgacgcgg atgatccagc
     5161 gtgacccgga actgagcggt gttggattgg tgatccttga tgagtttcat gagcgcagtt
     5221 tgcaggcgga tttggcgttg gcgctgttac tcgatgtgca acaaggtctg cgtgatgacc
     5281 ttaaactgct gattatgtcg gctacgctgg acaatgaccg cttgcagcaa atgctgccgg
     5341 aagcgcccgt cgtcatctca gaagggcgct cgtttccggt tgaacgccgt tatttaccgc
     5401 tgcccgcgca tcagcgtttt gacgaagccg ttgcggtagc caccgccgaa atggtgcgtc
     5461 aggaaagtgg atcattactg ttatttttac ctggcgtcgg agaaattcag cgcgtgcagg
     5521 aacaactggc ttcgcgcatc ggcagtgatg tggtgctctg cccgctgtat ggcgtgttgt
     5581 cactgaacga tcagcgaaaa gcgatcctcc cggcaccgca agggatgcgc aaagtggtgc
     5641 tggcgaccaa tattgctgaa accagtttaa ccatcgaagg tattcgtctg gtggtggatt
     5701 gtgcccagga gcgtgtggcg cgctttgatc cgcgcacggg gcttacgcgg ttgattactc
     5761 aacgcgttag tcaggcatcc atgacgcagc gtgccgggcg cgccgggcgt ctggagccgg
     5821 gtatcagcct gcatttaatc gccaaagaac aagcagaacg cgccgcggcg caaagtgagc
     5881 cggagatctt acaaagcgat ctttccggtt tgctgatgga attactgcaa tggggatgca
     5941 gcgatccggc gcagatgagc tggctggatc aaccgccaac ggtgaatcta ctggccgcga
     6001 aacgcctgtt acaaatgctg ggggcgctgg agggtgaacg gcttagtgcg caagggcaaa
     6061 aaatggcagc gctgggtaac gatccgcgtt tagcggcaat gctggttaac gcaaagagcg
     6121 atgacgaagc tgctaccgcg gcaaaaattg ccgccattct cgaagagccg ccacggatgg
     6181 gcaatagtga cctgggcgtg gcgttttcgc gcaatcaacc agcctggcag caacgtagtc
     6241 agcaactgtt aaaacgctta aacgtacgcg gcggtgaggc agacagttcg cttatcgcgc
     6301 cgctacttgc cggggcgttt gccgatcgca ttgctcatcg ccgtgggcaa gatggacgct
     6361 atcaactggc aaacagcatg ggagcgatgc tcgatgccga cgacgcgcta agccgccacg
     6421 aatggttgat cgcaccgtta ttattgcagg gcagcgcctc gccggatgcg cggattttac
     6481 tggcgctacc ggtcgatatt gatgaattag tacaacgctg tccgcagctg gtgcagcaat
     6541 ctgacaccgt ggaatgggat gacgcgcaag gtacgctgaa agcctggcgt cggctacaaa
     6601 tcggtcagtt gacggtgaaa gtgcagccgc tggcgaaacc gtcagaagac gagttgcatc
     6661 aggcgatgct taatggcatc cgtgataaag gtttaagcgt gctcaactgg acggcggaag
     6721 cggaacagct acgcttgcgt ttgttatgcg ccgcaaagtg gttgccggaa tatgactggc
     6781 cagcggttga tgatgaaagt ttgttggcga cgctggaaac gtggctgctg ccacatatga
     6841 ccggcgtaca ttcactacgc ggcctgaaat cactcgacat ttatcaggca ctacgcggat
     6901 tacttgattg gggaatgcag caacgtctgg atagtgaatt gcctgcgcat tacactgtgc
     6961 cgacgggaag ccggatcgcc attcgttatc atgaagataa cccgcccgcg ctggcggtga
     7021 gaatgcagga gatgtttggt gaggccacta atccgacgat cgcccagggg cgcgtgccgc
     7081 tggtgctgaa gttgctttcc cctgcccaaa ggccgctgca aatcacgcgt gatttggggg
     7141 ccttctggaa aggggcgtat cgtgaggtgc aaaaggagat gaaagggcgt tatcccaaac
     7201 acgtctggcc ggacgacccg gcaaacacgg caccgacgcg acggacgaaa aagtattcgt
     7261 aaggttggtt ttctccctct ccctgtggga gagggccggg gtgagggcat cagcg
//
LOCUS       DAZSNM010000203         7278 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZSNM010000203 DAZSNM010000000
VERSION     DAZSNM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.203"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..854)
                     /gene="bcr"
                     /locus_tag="ACHW42_003494"
     CDS             complement(<1..854)
                     /gene="bcr"
                     /locus_tag="ACHW42_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFR8945158.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSG"
     gene            complement(882..1577)
                     /gene="rsuA"
                     /locus_tag="ACHW42_003495"
     CDS             complement(882..1577)
                     /gene="rsuA"
                     /locus_tag="ACHW42_003495"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR8945159.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            1727..3487
                     /locus_tag="ACHW42_003496"
     CDS             1727..3487
                     /locus_tag="ACHW42_003496"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR8945160.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            3612..3896
                     /gene="rplY"
                     /locus_tag="ACHW42_003497"
     CDS             3612..3896
                     /gene="rplY"
                     /locus_tag="ACHW42_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR8945161.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(4035..5042)
                     /gene="yejK"
                     /locus_tag="ACHW42_003498"
     CDS             complement(4035..5042)
                     /gene="yejK"
                     /locus_tag="ACHW42_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR8945162.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            5224..5451
                     /locus_tag="ACHW42_003499"
     CDS             5224..5451
                     /locus_tag="ACHW42_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR8945163.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            5471..7231
                     /gene="yejM"
                     /locus_tag="ACHW42_003500"
     CDS             5471..7231
                     /gene="yejM"
                     /locus_tag="ACHW42_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR8945164.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
BASE COUNT         1820 a         1929 c         1819 g         1710 t
ORIGIN      
        1 cccgagcgga acatatttaa cgcgccaatg cggcggacga agcggctgtt aaagatggtc
       61 atcacgaaca gaaaaacaat gtttagcgca aagtaataac caaagttttc cggcgcgacg
      121 tggttaattt caatataaac aaacggtccg gcgcttaaga atgagaacat cccggcaaag
      181 ctgaaaccac tggcaagcat gtagctcagg acacgtttat ggcggaacag cgccgcaaag
      241 ttaccaatag tggtacgaat gtgaaatggc tgacgacgct ccggtggtaa ggtttcttta
      301 atcaggaaga aaatcattgc cgaagccaga atcgccgcta atgccaggat ccagaagatg
      361 taatgccagc tcagccacac cagcacccag ccgccaacta tcggtgccat cagcagtgca
      421 atggtcgtca ccagcatgac aaacgacatc atccgcgaga actcttcttt cgggtaaata
      481 tcacgcatca aagcgttgat aaccacgctg gccgcagccg cagccagccc gtggaagaaa
      541 cgcatcacaa tcagctgatc gatggtttgt gccaacgcac acgccaccgc ggcggcggca
      601 aacaccagcg tcccgccaag aaccaccggc ttacgcccga agctgtctgc catcggcccg
      661 tagattaact gccccaacgc aaagcccaga atataagtac tgagggtcat ctgcgtactg
      721 cccgccgata cgccaaactg cgctgaaatt accggtagcg cgggcagata catatcaatc
      781 gacagcggca tcaacatggc cagcaggcca aggataaaaa caatagcaaa cgacgaatgc
      841 tgtcgggtgg tcacaacggg ctcctgaaag tcattgaaaa gttagacgac gctggcaatt
      901 tcttcttcag ttaacggacg atattcaccg ggggctaaat cagcatccag cgtaataccg
      961 ccaatacgtt cacgatgcag ctcaaccacg tggttaccca cggcggcgaa catgcgtttc
     1021 acctgatgat aacgcccttc gctgatggtc agacgaacct gcgttggggt aatcacttca
     1081 agcaccgccg gcttagtgag atctttttcg ttatgcagct gcacgccttt agcaaattgc
     1141 tctgccgtat cgtcagctac aggtgattcc agtgtcacca gataggtctt ctcgcaatga
     1201 tggcgcggag aagtaatgcg gtgcgaccac tgaccatcat cagtcatcag caccagaccg
     1261 gtggtatcga tatccagacg tcctgcggcg tgcagtttcc acgctaccgg ttcatccaga
     1321 aaatagagca ccgtcgggtg atccgggtcg tccgtggagc aaacatagcc ctgaggctta
     1381 ttgagcatga agtaacgtgg accgtgttgc tgcgccagcg ggttgccatc gtaagcgaca
     1441 tcatgttcag ggagcagttt gaacgctgca ttacgaacga tttcgccatc gacggtgaca
     1501 cgattgccgc ggatttcacg cccggcaata gcacggctaa cgccgagttg ctgtgcgata
     1561 aatttatcaa gtcgcatgtg tgtgattttg cctgtaaaaa acggaggtcg ggtaatgacc
     1621 cgaaaatctg aactgttgtc tgcccagtat agcggtctaa taacttccct caagggaaaa
     1681 agaaccgtgg catactatta gcagaataat ctacctacgc gaaaccatga tttttacact
     1741 ccgcccatat cagcaagaag ccgtggatgc cacgctcaac cattttcgtc gtcataaaac
     1801 ccctgccgtt atcgtgctgc ccaccggcgc aggtaaaagc ctggtgatag cggaactggc
     1861 acggctggcg cgtggtcgcg tactggtgct ggcacacgtt aaagaactgg tggcgcaaaa
     1921 ccatgcaaag tatcaggcgc tggggctgga agccgatatt tttgccgccg ggctaaagcg
     1981 caaagagagc cacggtaaag tggtatttgg cagcgttcag tcggtcgccc gtaatcttga
     2041 tgcctttcag ggtgaatttt cgctgttgat tgtcgatgaa tgccaccgta ttggtgacga
     2101 tgaagagagc cagtatcagc aaatcctcac tcacctgact aaagtgaatc cccacttacg
     2161 cctgctgggg ctgactgcca cgccttttcg attgggcaaa ggctggatct accagttcca
     2221 ttatcacggc atggtacgcg gcgatgagaa agcccttttc cgtgactgca tttatgagct
     2281 gccgctgcgt tatatgatta aacacggcta tctgacgccg ccagaacgac tggatatgcc
     2341 agtagtgcaa tacgatttca gccgcttgca ggcacagagt aacgggctgt tcagcgaagc
     2401 tgatctcaac cgtgagctga aaaaacagca acgcattacg ccacatatca tcagccagat
     2461 catggagttc gccgaaaagc gcaaaggggt aatgattttc gccgccaccg ttgaacacgc
     2521 aaaagagatt gtgggattac tgcctgccga agatgctgca ctgattactg gcgacaaccc
     2581 cggcgctgag cgcgatgtgt taattgaaga ttttaaagcc cagcgttttc gctatctggt
     2641 caacgtcgca gtactgacca ccggatttga cgccccgcac gtcgatctta tcgccattct
     2701 gcgccctacc gaatcagtga gtctttacca acaaattgtc gggcgcggtc tgcgtctcgc
     2761 tccgggcaag actgattgtt taattcttga ttatgcgggt aatcctcacg atctctacgc
     2821 gccggaagtt ggtacaccga aaggcaaaag tgacaacgtt ccggtacagg ttttctgccc
     2881 tgcctgcggt tttgccaaca ccttttgggg gaaaacgacc gccgacggga cattgattga
     2941 acactttggt cgccgctgtc agggatggtt tgaagatgac gacggtcatc gcgaacagtg
     3001 tgacttccgt ttccgtttta aaaattgccc gcaatgtaac gcagaaaatg atattgccgc
     3061 tcgccgctgc cgcgaatgtg acacggtact ggttgatccg gatgatatgt taaaagcggc
     3121 gctacgactg aaagatgcgc tggtattacg ctgtagcggc atgtctttgc agcatgggca
     3181 cgacgagaaa ggcgaatggt tgaaaatcac ctattacgat gaagacggcg cggatgtgag
     3241 tgagcgtttc cgtctgcaaa cgcccgccca gcgtaccgcc ttcgagcagc tttttatccg
     3301 cccgcatacg cgcacaccgg gcatccctct gcgctggatc accgccgccg atatcctcgc
     3361 ccagcaagcc ttactgcgac atccggattt tgtcgtcgcc cgcatgaaag gtcagtactg
     3421 gcaagtgcgt gaaaaagtgt tcgattacga aggtcgtttt cgtcgggcgc acgaattacg
     3481 cggttaatgg tagttttgat tgatgtataa ggcgattgag tatagaatct cgcccgcttt
     3541 tgcatacgca aagcagatca cttacctgtt gctgggtcgc ctgtagcagg attaatttaa
     3601 gagagaaaga aatgtttact atcaacgcag aagtacgtaa agagcagggt aagggtgcga
     3661 gccgccgcct gcgtgccgct aacaagttcc cggcaatcat ctacggtggc aaagaagcac
     3721 cgctggctat cgagctggat cacgacaaag tcatgaacat gcaagctaaa gctgaattct
     3781 acagcgaagt tctaaccatc gttgttgacg gtaaagaaat caaagttaaa gctcaggacg
     3841 tacagcgtca cccgtacaaa ccgaagctgc agcacatcga cttcgttcgc gcttaattgc
     3901 tgaataagtt gtaaaaaacc ccgctccggc ggggtttttt gtatctgcag attatgcctg
     3961 attacggtat tgctattttt tgcaggccag ataaggcgtt cacgccgcat ctggcattca
     4021 gaacacaaaa ccgattaatt gccgccagac gtccggcgct gcaattggtc gcgcaaattc
     4081 ggcggcgtgc ctttaatggt caaggtatcg gttgctggat cccagaagat gcgttcacct
     4141 aacagcatcg catcaaagtt aatagtcaag ccaccgccgc taccggcaaa cttcgtcaac
     4201 tgacgcagtg tgctacggtc tgccgggaag ctctcttcca gttcgtagcc tttttcagcc
     4261 gcaaactccg tgaagctcac ttcgcttacg cctgccagct ctttcgacag cgattccagt
     4321 tcgatctctt cgcctgcctg cagctgctca ttacagtagc tataaacctg ctggcgcacg
     4381 ttctgtcgct ctgctttatc cagttgtgct tcggcagtga aatcatcaac cgcttgcaac
     4441 agcccacggt tttgcgcttt cgcgttcaat ccttcgctgg ccccgaggaa atccataaag
     4501 aaatccgcca ctttgcgccc tactcgccct ttcaggaaag tgagatagcg ggtggactct
     4561 ggattggttt cccattcggt taaatcgata cgcgcaacaa tatccgcatg attgatatca
     4621 agataatggg ttgggttgat gtcgagattt tcgttaacgc gcatactgct caggttgctc
     4681 agaactgcca ccagcaaata ctccaccgcc agataacggt agtggcaaaa cagcacgaat
     4741 ccaccgtcag caaaaggata tttcgctaat tcgtcacgca ggcgaccggt tgccgcgcgg
     4801 ctaaatgcca gaaagtcctc ttcgccctga cgctgtaagc gcagcgtctg cgccagttca
     4861 ctctcttcgc taaacaagcc gtaagcttta tttttggcgc tatagacccg atgcagttct
     4921 gccaccatct caacgacggt ttctgtcggt tccagcaatg aatcgcgcaa caccagctca
     4981 aggttctgct catcacgctt gataagctgg tgcagagcaa tctggttgat atccagactc
     5041 atgataaact ctcctttaag accgggcggt attcaaccac cgccagttaa gcgacgcaat
     5101 aaaagatggc ggtcatttta cagaaggcag cgtaacgaga atgatttaac gcataaaaaa
     5161 gcagaaaaaa aaccgttgct acggtaatat gttgcccttt catcaactaa ctgatttcga
     5221 tttatgccac aaatttcccg ctacagtaat gaacaggttg aacaattgct cgcagagctg
     5281 ctcaacgtac tggaaaaaca taaggctccg accgaccttt ccctgatggt gttaggcaac
     5341 atggtcacta accttatcaa caccagcatt gccccggccc aacgccaggc aattgccaac
     5401 tcttttgcca gcgccttaca gtcctctatc aacgaagaca aagcgcacta agggaaacag
     5461 ataacaggtt atggtaactc atcgtcagcg ctaccgtgaa aaagtctccc agatggtcag
     5521 ttgggggcac tggtttgcac tgttcaatat tctgctttcg ctcgtcattg gcagccgtta
     5581 cctgtttatc gccgactggc cgacaacgct tgctggtcgc atttattcct acgtaagcat
     5641 tatcggccat ttcagcttcc tagtgttcgc cacctacttg ctgatcctct tcccgctgac
     5701 ctttatcgtc ggctcccaga ggctgatgag gtttttgtcc gtcattctgg cgacggcggg
     5761 aatgacgcta ttactgatcg atagcgaagt ctttactcgt ttccatctcc atcttaatcc
     5821 catcgtctgg cagttggtta tcaacccaga cgaaaatgag atggcgcgcg actggcagct
     5881 gatgttcatc agcgtgccgg ttattttatt gcttgaactg gtgtttgcga cgtggagctg
     5941 gcaaaagctg cgcagcctga cgcgtcgtcg acgcttcgcg cgcccgctgg ccgcattctt
     6001 atttatcgcc tttatcgcct cgcatgtggt gtatatctgg gccgatgcca acttctatcg
     6061 cccgatcacc atgcagcgcg ctaacctgcc gctttcgtac ccgatgacgg cgcgacgttt
     6121 ccttgagaag catggcctgc ttgatgcgca ggagtatcaa cgccgtctta ttgagcaagg
     6181 taatccagac gccgtttccg tacagtatcc gttaagcgaa ctgcgctatc gcgatatggg
     6241 caccgggcag aatgtgctgt tgattactgt cgatggcctg aactactcac gcttcgagaa
     6301 gcagatgcct gcgctggcag gttttgctga gcaaaatatt tcgttcacgc gccatatgag
     6361 ctccggcaac actacagaca acggcatctt tggcctgttc tatggcatct cgccgagcta
     6421 tatggacggc attctgtcga cccgtacgcc tgcggcatta attactgcgc ttaatcagca
     6481 aggctatcag ctggggttat tctcatcaga tggctttacc agcccgctgt atcgccaggc
     6541 attgttgtca gatttctcga tgccgagcgt acgcacccaa tctgacgatc agaccgccac
     6601 gcagtggatc aactggctgg gccgctacgc acaagaagat aaccgctggt tctcgtgggt
     6661 ttctttcaat ggcactaaca ttgacgacag caatcagcag gcatttgcac ggaaatatag
     6721 ccgggcggca ggcaatgtcg atgaccagat caaacgcgtg ctcaatgcac tgcgtgattc
     6781 tggcaaactg gacaatacgg tggtgattat cactgccggt cggggtattc cactgagcga
     6841 agaggaagaa acctttgact ggtcccacgg tcatctgcag gtgccattag tgattcactg
     6901 gccaggcacg ccggcgcagc gtattaatgc tctgactgat cataccgatc tgatgacgac
     6961 gctgatgcaa cgcctgctgc atgtcagcac acctgccagc gaatattcgc aaggtcagga
     7021 tttgttcaac cctcaacgcc gtcattactg ggttactgct gcggataacg atacgctggc
     7081 aattaccacc ccgaaaaaga cgctggtgct gaacaataac ggtaaatacc gcacttacaa
     7141 cttacgtggt gaaagagtga aagatgaaaa accacagtta agtttgttat tgcaagtgct
     7201 gacagacgag aagcgtttta tcgctaactg attaattata aatcagttag cgaaatatct
     7261 tacttgcaat cggtgtgg
//
LOCUS       DAZSNM010000204         7211 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZSNM010000204 DAZSNM010000000
VERSION     DAZSNM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.204"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHW42_003501"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHW42_003501"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR8945165.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHW42_003502"
     CDS             complement(1672..2667)
                     /locus_tag="ACHW42_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR8945166.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHW42_003503"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHW42_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHW42_003504"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHW42_003504"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR8945167.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHW42_003505"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHW42_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR8945168.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..>7211)
                     /gene="emrK"
                     /locus_tag="ACHW42_003506"
     CDS             complement(6108..>7211)
                     /gene="emrK"
                     /locus_tag="ACHW42_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR8945169.1"
                     /translation="VIVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGS
                     VTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEV
                     ASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKAL
                     VMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGYIAQRSVQVGETVSSGQSLMA
                     VVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLL
                     PAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKDEDIAEMPDLAST
                     VTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         1990 a         1713 c         1664 g         1844 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca c
//
LOCUS       DAZSNM010000205         7209 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZSNM010000205 DAZSNM010000000
VERSION     DAZSNM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7209
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.205"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            217..1788
                     /gene="garD"
                     /locus_tag="ACHW42_003507"
     CDS             217..1788
                     /gene="garD"
                     /locus_tag="ACHW42_003507"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR8945170.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(1840..2664)
                     /gene="agaR"
                     /locus_tag="ACHW42_003508"
     CDS             complement(1840..2664)
                     /gene="agaR"
                     /locus_tag="ACHW42_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR8945171.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            2913..4193
                     /gene="kbaZ"
                     /locus_tag="ACHW42_003509"
     CDS             2913..4193
                     /gene="kbaZ"
                     /locus_tag="ACHW42_003509"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR8945172.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            4216..4689
                     /gene="agaV"
                     /locus_tag="ACHW42_003510"
     CDS             4216..4689
                     /gene="agaV"
                     /locus_tag="ACHW42_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR8945173.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            4700..5479
                     /gene="agaW"
                     /locus_tag="ACHW42_003511"
     CDS             4700..5479
                     /gene="agaW"
                     /locus_tag="ACHW42_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR8945174.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            5469..6278
                     /gene="agaE"
                     /locus_tag="ACHW42_003512"
     CDS             5469..6278
                     /gene="agaE"
                     /locus_tag="ACHW42_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR8945175.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLNGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            6365..6799
                     /gene="agaF"
                     /locus_tag="ACHW42_003513"
     CDS             6365..6799
                     /gene="agaF"
                     /locus_tag="ACHW42_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR8945176.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            6796..>7209
                     /locus_tag="ACHW42_003514"
     CDS             6796..>7209
                     /locus_tag="ACHW42_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR8945177.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         1712 a         1870 c         1915 g         1712 t
ORIGIN      
        1 aaatattatt ttgagcatat gcacataagg ttgcgcgcta aagcacagat ttgcgcttta
       61 ccttgccggg cggcactgca atccctaaaa tgattgacat tgatcacatt tctgcgttta
      121 aactcctgac attcttattt cacccaatga aatcatttat ttttaaatga gaccaggtcc
      181 tcattttaat aacccctggc tggagaatat tgcacaatgg ccaacatcga aatcagacaa
      241 gaaacgccaa ctgcgtttta tataaaagtt cacgacacag ataatgtggc aattattgtt
      301 aatgataatg gcctgaaagc aggaacgcgt tttccggatg ggctggaatt aattgaacat
      361 attccccagg ggcataaagt cgcattgctg gacattccgg ctaatggtga aattattcgt
      421 tatggcgaag tgattggtta cgccgtgcgt gcaatcccac gcggaagctg gatcgacgaa
      481 tcaatggttg tactaccgga agcgccgccg ttacacacgc tgccactggc aaccaaagtc
      541 ccggaaccct taccgccgct ggaaggatac acctttgagg gctatcgcaa tgccgatggc
      601 agcgtgggca ccaaaaacct gctcggcatc accaccagcg tccactgtgt ggcaggcgtg
      661 gtggactatg tagtaaaaat cattgaacgc gatctgctac cgaaataccc gaacgtcgat
      721 ggcgtggtgg ggctgaatca tttgtacggt tgtggcgtgg cgattaacgc accggcagca
      781 gttgtaccta ttcgtaccat tcacaatatt tcgctgaacc ctaactttgg cggcgaagta
      841 atggtgattg gcctgggttg tgaaaagttg cagcctgagc gcctgctgac tggaacggat
      901 gatgtgcaag ctattccagt agaaagcgcc agcattgtca gtttgcagga tgaaaagcat
      961 gtcggttttc agtccatggt cgaggatatt ttgcaggtcg ccgaacgcca tctacaaaaa
     1021 ctgaatcaac gtcagcgaga aacctgtccg gcttcagaac tggttgtcgg catgcattgc
     1081 ggtggcagcg atgcgttttc tggcgtaacg gcaaacccgg cggttggcta tgcgtctgat
     1141 ctactggtgc gctgcggcgc aacggtgatg ttttcagaag tcacggaagt gcgtgacgcg
     1201 atccatctgc tgacaccacg cgcagtgaac gaagaggtcg gcaaacggct gctggaggag
     1261 atggagtggt acgataacta tctcaatatg ggaaaaaccg accgcagcgc caacccttcg
     1321 ccgggcaaca agaaaggcgg tctagcaaac gtggtagaga aagcgctcgg ctccattgct
     1381 aaatcgggca aaagtgcaat tgttgaagtg ctgtcgcccg gtcaacgccc gactaaacgc
     1441 ggattaattt acgccgcgac gccagccagc gattttgtct gtggcacgca acaggtggct
     1501 tcgggtatca ccgtgcaagt gtttacgacc ggtcgtggta cgccgtacgg cctgatggcg
     1561 gtacccgtca ttaaaatggc accccgcacc gagctggcga accgctggtt tgatttaatg
     1621 gatattaatg cgggcaccat cgctaccggc gaagaaacta ttgaagagga gggctggaag
     1681 ttgttccact ttattctcga cgtcgccagc gggaagaaga aaaccttctc ggatcaatgg
     1741 ggactgcata accagctggc ggtgtttaac ccagcaccgg tgacctgata attcaattcg
     1801 tattatgccc gatagcacac gtttatcggg catattgagt cacagcgaag acgcgtgatc
     1861 cccgaccaga atcacttcaa ccccagcctt tcgcagtcct tccagactat ccgcaggaat
     1921 gccttcatca acaatgatca tgtcgatacg ttgagtatca atgatcttat gtaaactgga
     1981 acgattgaat ttactggaat cggtgaccac gatgatccgt tccgcaactt cgcacatccg
     2041 acggcttaaa cgggcttcat cttcattatg tgtgctgacg ccgcgctcca gatcgatcgc
     2101 atctacacca agaaacagca tatcgaagtg gtaattttgc agcgattgct cagcctgatc
     2161 gccgtaaaaa gattgcgact gacggcgcaa atgcccgccg gtcatcagca gctcaacgcc
     2221 ttccgcttcc agcaacgcat tagccacgtt cataccgttg gtcatcgcaa ttacgtcagt
     2281 gtacttgcgc atcagacgag caatctcaaa agtggtggtc ccggaatcga ggatcacccg
     2341 atgacctggc tgaatcaact caacggcagc tttcgcaacg ctgcgtttca tcgcggtgtt
     2401 cagtgcgctt ttatcttcca ctgatggctc gactgacggc gtcgtgctat cgcagatcaa
     2461 cgcgccacca taggcacgca cagcgatccc ctgcttttcc agaaacgcca gatcattgcg
     2521 gatcgtcaca gtagatacgc catacaatgc cgacagatcg ttaacctgca cactcccttg
     2581 ctgtcgcaga cgctgaatga tctgttctcg tcgctcgctg gtgcctgtca ctcgcttctc
     2641 acctgaagcg tcggtattac tcatagtaag tcctttcgta aaactttcgt ttcatttcgt
     2701 tttgcctatt aacgcctttc tattaagcaa atgcaagccc accttgccca ttggcgcaag
     2761 ctactctcgt ttcagtgact ttcattacgt ttcttttgtg aatcagatca gaaaactatt
     2821 atctttcgtt ttatttttat ctcaccatga cgcagtatca attgaaacaa aacgaaagat
     2881 caatatcgca gtaatctgaa ctggagagga aagtgaaaca tctgacagaa atggtgagac
     2941 agcacaaagc gggcaaaaca aatgcaattt atgccgtttg ttccgcacat ccgctggtgc
     3001 tggaggctgc aatccgctac gccagtgcaa accaaacgcc gttactgatt gaagcaacct
     3061 ccaatcaggt agaccagttc ggcggttata ccggaatgac gcccgccgat tttcgcggct
     3121 ttgtttgtca gctcgccgac tcgttgaatt tcccgcagga tgcgctgatt ctgggtggtg
     3181 accatctggg gccaaaccgc tggcaaaacc tgccagccgc tcaggcaatg gccaatgccg
     3241 atgatttgat taaaagctac gttgcggcag gattcaaaaa aatccacctt gattgcagca
     3301 tgtcctgtca ggacgatcca attcccttaa ctgatgacat cgtggctgaa cgcgccgccc
     3361 gtctggcgaa agtggcggaa gaaacctgtc gcgaacactt tggcgaagcc gatctggagt
     3421 atgtcattgg taccgaagtg ccggtacctg gcggcgcgca tgaaacctta agcgagctgg
     3481 cggtcaccac gccggatgcc gcccgcgcca cgctggaagc ccatcgtcac gcctttgaaa
     3541 agcaaggttt gaatgccatc tggccacgca ttattgccct ggtggttcaa cccggcgtcg
     3601 aattcgatca caccaacgtt attgattatc agcccgccaa agcgagcgcc ttaagccaga
     3661 tggtcgaaaa ctacgaaacg ctgattttcg aagcgcactc taccgattat caaacgccgc
     3721 aatctctgcg ccagctggtg attgaccact ttgccattct gaaagttggc ccggcgctga
     3781 ccttcgccct gcgtgaagcc cttttttctc tggcggcgat tgaagaagaa ctggtaccag
     3841 cgaaagcctg ttccggtctg cgtcaggtgc tggaaaacgt gatgctcgac cgcccggaat
     3901 actggcaaag ccactaccac ggtgacggca acgcgcgtcg tctggcgcgt ggttacagct
     3961 actcagatcg cgtgcgctat tactggccgg acagccagat tgatgacgct ttcgctcatc
     4021 tggttcgtaa tctggcggat tcaccaattc cgctaccgct catcagccag tatctgccgc
     4081 tgcagtacgt gaaagttcgc tccggcgagc tgcaaccgac gccacgggaa ctcattataa
     4141 accatattca ggacatcctg gcgcagtacc acacagcctg tgaaggccaa taagcaaaac
     4201 aaagaggaac atgctatgcc aaatattgtt ttaagccgga ttgatgaacg cttgattcac
     4261 ggtcaggtcg gcgttcagtg ggtcggattt gcgggggcaa atctggtgct ggtagccaac
     4321 gatgaggttg ccgaagatcc ggtacaacaa aacctgatgg aaatggtact ggcagaaggg
     4381 atcgccgtac gtttctggac gctgcaaaaa gttatcgaca acattcatcg cgccgccgat
     4441 cgacagaaaa tcctgctggt ttgtaaaaca cccgccgatt tcctgacgct ggtgaaaggt
     4501 ggcgttccgg tgaatcgcat taacgttggc aatatgcact acgccaatgg caaacaacaa
     4561 atcgccaaaa cggtttctgt ggatgcgggc gatatcgcag catttaacga cctgaaagcc
     4621 gctggggtgg aatgcttcgt tcagggcgtc ccgacagagc ctgctgtgga cctctttaaa
     4681 ttactttgag ggattcatca tggaaatcag cctgttgcag gcatttgcgt tgggcattat
     4741 cgcctttatc gctggcctag atatgtttaa cggcttaacc catatgcacc gcccggtggt
     4801 cctcggcccg ttggtcgggc tggtacttgg cgatctgcat accggaattt taaccggcga
     4861 tacgctggaa ctagtgtgga tggggctggc cccgctggcg ggcgcacagc cgcctaacgt
     4921 gattatcggt actatcgtcg gcacggcgtt tgccattact actggcgtga aacccgatgt
     4981 cgcggtcggt gtcgccgtac ctttcgctgt cgcagtacag atggggatta ccttcctgtt
     5041 ctcggtgatg tctggcgtga tgtctcgctg cgaccggatg gcggagaacg ccgatactcg
     5101 cggcattgaa cgcgtgaact atctggcact gctggcactc ggcactttct attttctctg
     5161 cgctttcctg cctatctact tcggcgcgga acatgcaaaa accatcattg atgtcctgcc
     5221 gcaacgatta atcgacggcc tcggcgtcgc gggcggcatc atgccagcaa tcggttttgc
     5281 cgtgctgctg aaaatcatga tgaaaaacgt ctacataccc tacttcatcc tgggctttgt
     5341 tgccgcaacc tggctcaagt taccggtgct ggctattgct gccgccgcgc tggcgatggc
     5401 gctgatcgat ctgctgcgta aatctcctga accgactcaa cctgcggcac agaaagagga
     5461 atttgaagat ggcatctaat caaaccaccc tgccgaacgt cagcgaaaat gaagaaacac
     5521 tgctgactgg cgtcaatgaa aacgtgtatg aagatcggag cattgccgcg gagctgacga
     5581 aaaaagatat caaccgcgtc gcctggcgtt ccatgctgtt acaagcgtct tttaactacg
     5641 aacgtatgca ggcttctggc tggttgaacg gtctgctgcc tgctctgaaa aagatccaca
     5701 ctaataaacg cgacctggcg cgcgccatga aggggcatat gggtttcttc aatacccatc
     5761 cgtttctggt gacgtttgtt atcggcatta tccttgcgat ggagcgttct aagcaggacg
     5821 ttaacagtat tcagagcacc aaaattgccg tcggtgcgcc gcttggcggg attggcgatg
     5881 cgatgttctg gctaacgcta ctgccgattt gtggcgggat aggcgcaagc ctcgcgctac
     5941 aaggctctat tcttggcgca gtcgtcttta ttgtgctgtt caacgtggta cacttggggc
     6001 tgcgttttgg tctggcgcat tatgcttacc gcatgggcgt ggcggcgatt ccccttatta
     6061 aagcgaatac caaaaaagtc ggccatgctg cgtcaatcgt tggaatgacg gtaatcggcg
     6121 cactggtggc aacctatgtt cgtttaagca ccacgctgga aatcacggcg ggcgacgcag
     6181 tagttaagtt acaggctgat gttatcgaca aactgatgcc tgccttctta ccgctggtct
     6241 acaccctgac catgttctgg ctggtacgcc gcggctagag tccgctgcgc ctgattgcgg
     6301 ttaccgtggt tctagggatc gtcggtaaat tctgccattt cctttaaata caatgaggta
     6361 ttagatgtta agtattattt tgacagggca tggcgggttt gccagtggca tggaaaaagc
     6421 aatgaagcaa atcctcggcg atcaatcgca gtttattgcc atcgattttc ccgaaacctc
     6481 aagcgccgcg ctgcttacat cgcagcttga agaggccatc gcccaactgg attgtgaaga
     6541 cggcattgtt tttctgacgg atttgctggg cggcacgccg tttcgcgtag cttcaacgct
     6601 ggcgatgcaa aaaccgggct gtgaagtgat taccggcacc aatttgcaac tgctactgga
     6661 gatggtgctg gagcgcgaag ggttaagcgg tgaagaattc cgtgtacagg cgctggagtg
     6721 tggacaccga gggctgacca gtctggtgga cgagttaggc cgctgccatg aagaatgtcc
     6781 ggtcgaggaa ggaatatgac acacgttctg cgcgccagaa ggctgctgac tgaagaggga
     6841 tggttcgatg accatcagtt gcgtgttgct gacggtgtca tcgcagcaat cgaaccgatt
     6901 ccagtgggcg tgactgaacg cgatgcggaa cggctctgcc ccgcttacat cgacacccat
     6961 gtacacggtg gtgcgggcgt tgatgtaatg gatgacgcgc cggatgtact cgacaagctg
     7021 gcaatgcaca aggcacgcga aggtgtcggc agttggttac cgaccaccgt aaccgcgccg
     7081 cttaatacca ttcatgcggc gctgaaacgt attgctcaac gttgccaacg cggcggacct
     7141 ggtgcgcaag tgctggggag ttatctcgaa ggaccgtact tcacgccgca gaataaaggc
     7201 gcgcatccg
//
LOCUS       DAZSNM010000206         7187 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZSNM010000206 DAZSNM010000000
VERSION     DAZSNM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.206"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            27..878
                     /gene="hchA"
                     /locus_tag="ACHW42_003515"
     CDS             27..878
                     /gene="hchA"
                     /locus_tag="ACHW42_003515"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR8945178.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(986..2344)
                     /gene="hprS"
                     /locus_tag="ACHW42_003516"
     CDS             complement(986..2344)
                     /gene="hprS"
                     /locus_tag="ACHW42_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR8945179.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="ACHW42_003517"
     CDS             complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="ACHW42_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR8945180.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3148..3561
                     /gene="hiuH"
                     /locus_tag="ACHW42_003518"
     CDS             3148..3561
                     /gene="hiuH"
                     /locus_tag="ACHW42_003518"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR8945181.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3670..4674
                     /gene="msrP"
                     /locus_tag="ACHW42_003519"
                     /pseudo
     CDS             3670..4674
                     /gene="msrP"
                     /locus_tag="ACHW42_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4675..5310
                     /gene="msrQ"
                     /locus_tag="ACHW42_003520"
     CDS             4675..5310
                     /gene="msrQ"
                     /locus_tag="ACHW42_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR8945182.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5568..6218
                     /gene="zinT"
                     /locus_tag="ACHW42_003521"
     CDS             5568..6218
                     /gene="zinT"
                     /locus_tag="ACHW42_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR8945183.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2032 a         1629 c         1507 g         2019 t
ORIGIN      
        1 aactaagcaa caataaggaa tacactatga ctgttcaaac aagtaaaaac ccgcaggtcg
       61 atattgctga agataatgcg ttcttccctt cagaatattc gcttagccaa tatacgagtc
      121 ccgtctctga tcttgatggc gtggactatc caaaaccgta tcgcggtaaa cataaaattc
      181 tggtgatcgc cgcggacgaa cgttatttgc cgaccgataa cggaaaactg ttctcgaccg
      241 gtaaccatcc gattgaaacg ttgctgccgt tgtatcatct ccatgctgca ggtttcgaat
      301 tcgaagtggc gaccatttcc ggtctgatga ccaagtttga atactgggct atgccgcaca
      361 aagatgaaaa agtaatgcca ttctttgaac agcataaatc gttattccgt aatccgaaga
      421 aactcgcgga tgttgtcgcc agcctcaacg ctgatagcga atatgcagca atctttgttc
      481 ctggtgggca tggcgcactt attggtttac cagaaagcca ggacgtggct gccgctttac
      541 agtgggcaat caaaaatgac cgttttgtta tctccctttg ccacggtccg gcggcttttc
      601 tggcacttcg ccacagcgat aacccactga atggttattc tatttgcgca ttcccagacg
      661 ccgcagacaa acaaacgcca gatattggct atatgccggg tcatctcacc tggtacttcg
      721 gcgaagagtt gaagaaaatg ggcatgaaca tcattaatga cgacatcacc gggcgagtac
      781 ataaggaccg taaacgtctc accggcgaca gtccttttgc agcgaatgcg ttgggtaaac
      841 tggcggcgca ggaaatgctg gcagcttacg cgagttaatc cctgttacat tgtgatgtaa
      901 gcgcataatc aatcgcagcc acgttaatac tcacgacgtg gctgcgtttt ttcacggtta
      961 acttatggcg tactgaagcc cgatgttaat ttctttgcgg taacgtaatt cggaacacgt
     1021 tatgcttact gagatagtga tacgtagcac ttcctccatg taattcggca atcgctttga
     1081 ctaaagaaag gccaagtccc tgacctacgg aatgacgcga attatctccc cgccaaaatc
     1141 tacggaagag tttttcaggc tcattaattt tcgttccagg actggcgata tcaatattaa
     1201 gagagccgtt ggcatcaaga aaactggtta tatgaatacg cgatttttct ggtgaatatc
     1261 gaatggcgtt aacaataaga ttcgataaca ttcgttgcag taaaatttta tccgcaaaga
     1321 tttgttgatt gcacttgacc ttaaagcaaa tctctttctc gtctgaaaga tattcaagat
     1381 agtccaaaag attttcaact tccttattga gagaaagcga atccagtttc accaaaacat
     1441 tgtttttgtc tgccctggca agaaacagta tattctctgt taaccgcgaa atattttcca
     1501 gctcttcaat gtttccggca attgtttttt gatattcagc gatacttctg atttgactga
     1561 gcgtaacctg attctgaccc agtaatgcat taattggcgt tctaagttca tgagcgagat
     1621 cgtcagcaaa ttgactaagg cgctcaaaat ctttgactaa agcatgatgc attttattca
     1681 acgcctgccc aagaggtttt agttctctcg gtaatgcgtt aacctcaacc ggcactcggc
     1741 tatcgttata attcagcgct tccgttacgc cactcaactt tttgatctct cgcaaccccg
     1801 tcctgattaa caacggactt aatactgagc acagtataat ggcgacaatg caaattataa
     1861 tgctattaat tttatactgt tcaagcatat tatgtctggc tgaagccaat ttagccacag
     1921 taaccgttaa tgatggcgaa acttcatcaa tgttaattcg taaagcatct atctctgtat
     1981 cattaataat gcttctgtaa atgcctgctg cgctgattgt ctcactagca ggtatgttat
     2041 ttaacatgtc atcactgaca tttgtccggt taacaatttt attgatgcca tcactatgaa
     2101 tgatcaaaat atcctgacta acatccatca tccggttaaa gtacacaggt aacgtatctg
     2161 gatttacccc atcaattaac aactgcttga tctgcgctgt ccggttaatg agtgttgtat
     2221 catcgcgcca tttcaactca cttgccaggc cattatagag agtccataca attccagcgc
     2281 cagcaacaga ctgcagaaat ataaaaagca aggttaaacg gacggttata gataatcttt
     2341 tcattatttt tttaccgcta cgaatgaata gcccatcccc cggattgtgg cgattagctt
     2401 ttcaggaaaa ggatcatcaa cttttgcgcg gagcctgcga atggcgacat ccaccgtatt
     2461 ggtatcacta tcaaagttga ttccccaaat ttcactcgca ataaccgttc tgggtataat
     2521 ctcgccagct ctggaggcca gtagccaaag taactgaaac tccttgcgcg tcagtgtaat
     2581 actgatattg tccctgctga cactttgact gacagagtcc atttttaagc cactgatttc
     2641 taatgttgaa ttcagagcgt gatgttgcct taattgtgcc cgaacccttg ctagcaactc
     2701 agaaaatgaa aaaggtttta ccagataatc atttgcccca ctgtccaacc ctctgactct
     2761 gtcatcgaca gaatcccttg cagtaaggca aataacaggg gtttgctttg ccgttcttaa
     2821 cgtttgtaag atctgccagc catccatacc cggaagcata atatccagaa tgatcaatgc
     2881 ataatcatcc ttcagcgcaa gataaagccc atctctgcca tcagaaacgg catcaatgac
     2941 ataacccgct tcggaaagcc cctgcgttac ccattcctgg gtcctttgat tatcttcaat
     3001 aagtagaatc ttcatgaaaa taatatgcca taaatcatat gttatagaaa caacctggtt
     3061 cattacaaaa ttgtaatgct gctgtaaggt taccctggac gttttttcgc tatcctcaaa
     3121 actcattcac atgacaagga tataaacatg ttaaagcgtt atttagtact ctcggtagca
     3181 acggcagcct tttcattacc ttctttggtt tatgccgcac aacaaaacat tcttagcgtg
     3241 cacattttga accagcaaac aggaaaaccc gctgccgacg tgacagtcac tcttgaaaag
     3301 aaagcggata acggctggtt acaacttaat accgccaaaa cagataagga tggacgaatt
     3361 aaggcactgt ggcccgagca aactgcgact actggcgatt accgtgtcgt atttaaaacc
     3421 ggggactatt tcaagaaaca aaatcttgaa agtttcttcc ctgagattcc cgttgaattt
     3481 catattaata aagtgaacga gcattatcat gtgcctttgt tactcagcca atatgggtat
     3541 tcaacctatc gtggcagtta atgcgtaaat atgtaaaagc tgatctctgc tactgttagg
     3601 gaggtgactc cctgacagat taacagtaaa cagctcttgt tggttaacga caaaaaagtg
     3661 tgatggctta tgaaaaagaa gcaattttta aaagaatcag atgttacggc cgagtcggta
     3721 ttctttatga agcgtcggca ggtgttaaaa gcactgggca tcagcgcagc tgcattttct
     3781 ttgcctcacg ctgcgcatgc cgatctgctt agctggttta aagggaacga tcgtccgccc
     3841 gcccccgccg gaaaaccgct ggagttcagc aagcctgccg cctagcaaaa taacctgcca
     3901 ctgacgccag cagataaagt ctccggttat aacaacttct atgaattcgg gctggataaa
     3961 gacgatcccg cggctaatgc tggtagcctg aaaaccgatc catggacact gaaaatcagc
     4021 ggcgaagtgg caaaaccatt gaccctcgat catgatgatt taacccgtcg cttcccgctg
     4081 gaagagcgta tttatcgtat gcgctgcgtg gaagcgtggt cgatggtggt gccgtggatt
     4141 ggttttccgc tacacaaatt gctggcgctt gccgaaccca ccagcaatgc gaagtatgtc
     4201 gctttcgaaa cgatttatgc gccggaacag atgcctggcc agcaggaccg ctttatcggc
     4261 ggcgggctga aatatcctta tgtcgaagga ttgcgtctcg acgaagcaat gcatccgctc
     4321 acactgatga ccgtaggtgt ttatggcaag gcgttaccac cgcaaaatgg cgcgccggtg
     4381 cgactgattg tgccgtggaa atatggcttt aaagggatta aatcgatcgt cagtattaag
     4441 ctgacccgcg agcgtccgcc aaccacctgg aatctggcag cgcctgacga atacggtttt
     4501 tacgccaacg ttaatccgca tgttgatcac ccgcgctggt cacaggctac cgaacgattt
     4561 attggttcag gcggcattct cgatgttcag cgccagccaa cactactgtt taatggttac
     4621 gccgaccagg tggcatcgct gtatcgtggc ctggatttgc gggagaattt ctaaatgcgt
     4681 ctgacagcaa aacaagtgat atggctgaaa gtttgcctgc atcttgccgg attgttgccg
     4741 tttctctggc tggtctgggc gatcaatcac ggtggactgg gtgccgatcc ggtgaaagat
     4801 attcagcatt ttaccggtcg cactgcactg aaatttttgc ttgcggcctt gttaatcaca
     4861 cctctggcac gctacgcaaa acagccgtta ttgatacgca ctcgccgcct gttaggctta
     4921 tggtgctttg cctgggcgac actgcattta accagttacg cattgctgga gttgggcgtg
     4981 aataatctgg cgttattagg taaggagtta attacccggc cttatttaac gttaggaatt
     5041 atcagctggg ttattttgct tgctttagcg ttcacttcaa cccagtcgat gcagcgaaaa
     5101 ctcggcaaac actggcaaca gttgcataac ttcgtctatc ttgtcgcgat cctggcccca
     5161 atacattatc tgtggtcagt gaagattatt tcaccgcagc cactcatcta cgccgggctg
     5221 gctgtactgc ttttagcctt acggtataag aagttgcttt cccttttcaa ccggttacgc
     5281 aagcaggccc acaacaaatt atctctataa cttcatattg ccgacaaact tcgcgttatc
     5341 ccggccggat gcggcgcgag cgccctatcc ggcccacaca gatcgcatca agttatgttt
     5401 cgtttgtcat caacctttca tcaccagaat aatttatttt atttcaataa gatagttcaa
     5461 tacacctgag aataccatgc tctcatttcc taatgagttg ttgcattttt tcatatgtta
     5521 caatataaca ttacacataa tgtacattaa ctctggagga aattgttttg gcgattcgtc
     5581 ttcataaact ggctgttgct ttaggtgtct ttattgttag cgctcctgcc ttttcgcatg
     5641 gtcatcattc tcatggcaaa cccttaacag aggtcgaaca aaaagctgct aatggtgttt
     5701 ttgatgatac caatgtacaa aaccgaacgc tcagtgactg ggatggagtc tggcaatccg
     5761 tttacccttt actgcaaagt ggcaaacttg atcccgtctt tcagaagaaa gcggatgcag
     5821 ataaaaccaa aacatttgct gaaattaaag attattatcg caaaggttat ccgacagata
     5881 tcgaaatgat tggcattgag gacggcattg tcgaattcca tagaaataat gaaacaacat
     5941 cctgtaaata tgattacgat ggatacaaaa tactcaccta taaatcaggc aagaaaggcg
     6001 ttcgttactt attcgaatgt aaagatcctg aaagcaaagc ccctaaatat atacaattta
     6061 gcgatcatat aattgcacca cgaaaatcat ctcattttca catttttatg ggtaatgatt
     6121 cccagcaatc tttattgaat gaaatggaaa actggccaac gtattatccc tatcagttga
     6181 gtagcgaaga agtggtcgag gagatgatgt ctcattgatt tttttgcgct gacttcagtt
     6241 atttaacaga acagtgcctg gtagtaacaa caataattta ccaagacaaa taagtaagac
     6301 aaactcagac acgctcatac ttgagtcgtg tcttttttat tatgtctgta gaaaaaaact
     6361 gagaaaaagg ctaacaataa atacacaact cagccataca gactggcagc gcaaatgttt
     6421 ccaggagcaa gtcatctgtc atggtaaagc taaccagaaa tataggttaa ttttggtgtt
     6481 taaacctttt ttacttgtta gtatgataca aaggctttca aaaaagctgg gaaattcaac
     6541 aactcttgga aattatggac tttgtcccgc ggctttaatc tgacggccgc gttccttttt
     6601 tggctcaatt attgacccct tctctacgca acttcagttc ccgccagcaa ctttgcggca
     6661 gtttcatagg atcaatgtct aaaagaataa tggtgaccga taagaaaacg actgaataac
     6721 tgtagatttt cgctcgaaac cttcctgtca gctccatagc gaatcaagcg ctgaatgtca
     6781 cagtatcgaa cagaaaacag tgacgatcta acccttcaag aatattctac gattgttctg
     6841 tttaggaaaa gcaaggcggg aagtcgggag ataagtcatt gataaaatgg cggagagagg
     6901 gggatttgaa cccccggtag agttgcccct actccggttt tcgagaccga tgatacagag
     6961 tcgaaaaatc aaagagttaa aaatttttct tggaaaaaat gtcttaaaat cgtacacgaa
     7021 aaatcaatga attacataac attaaagaag aatattccaa gccttcttga gtccaccacc
     7081 tgttttcagg tgtcattcag acgtgatttg ccccagattg ggaaagcacg cttttttctc
     7141 agcttattcc gttttgcctg tgtgttgaat aattccactg cagttat
//
LOCUS       DAZSNM010000207         7183 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZSNM010000207 DAZSNM010000000
VERSION     DAZSNM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.207"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(371..1198)
                     /locus_tag="ACHW42_003522"
                     /pseudo
     CDS             complement(371..1198)
                     /locus_tag="ACHW42_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHW42_003523"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHW42_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFR8945184.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHW42_003524"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHW42_003524"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFR8945185.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHW42_003525"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHW42_003525"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFR8945186.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHW42_003526"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHW42_003526"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFR8945187.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHW42_003527"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHW42_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="ACHW42_003528"
     CDS             7090..>7183
                     /locus_tag="ACHW42_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HFR8945188.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1676 a         1812 c         1876 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAZSNM010000208         7135 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZSNM010000208 DAZSNM010000000
VERSION     DAZSNM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.208"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..146)
                     /locus_tag="ACHW42_003529"
     CDS             complement(<1..146)
                     /locus_tag="ACHW42_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945189.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSL"
     gene            224..1318
                     /gene="ddlA"
                     /locus_tag="ACHW42_003530"
     CDS             224..1318
                     /gene="ddlA"
                     /locus_tag="ACHW42_003530"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR8945190.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1342..1554)
                     /locus_tag="ACHW42_003531"
     CDS             complement(1342..1554)
                     /locus_tag="ACHW42_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR8945191.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1814..2122
                     /locus_tag="ACHW42_003532"
     CDS             1814..2122
                     /locus_tag="ACHW42_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR8945192.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2181..3275)
                     /gene="yaiW"
                     /locus_tag="ACHW42_003533"
     CDS             complement(2181..3275)
                     /gene="yaiW"
                     /locus_tag="ACHW42_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR8945193.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3288..4508)
                     /gene="sbmA"
                     /locus_tag="ACHW42_003534"
     CDS             complement(3288..4508)
                     /gene="sbmA"
                     /locus_tag="ACHW42_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR8945194.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4860..6017
                     /gene="ampH"
                     /locus_tag="ACHW42_003535"
     CDS             4860..6017
                     /gene="ampH"
                     /locus_tag="ACHW42_003535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR8945195.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6018..6521)
                     /locus_tag="ACHW42_003536"
     CDS             complement(6018..6521)
                     /locus_tag="ACHW42_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8945196.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(6609..>7135)
                     /locus_tag="ACHW42_003537"
     CDS             complement(6609..>7135)
                     /locus_tag="ACHW42_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR8945197.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         1809 a         1826 c         1726 g         1774 t
ORIGIN      
        1 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
       61 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
      121 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
      181 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
      241 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
      301 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
      361 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
      421 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
      481 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
      541 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
      601 gtttgtaggt tctgatgttc tggcttcagc agcctgtatg gataaagatg tcaccaaacg
      661 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
      721 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
      781 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
      841 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
      901 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
      961 gatcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     1021 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     1081 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     1141 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     1201 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt
     1261 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     1321 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     1381 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     1441 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     1501 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     1561 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     1621 cattttcgta attattaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     1681 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     1741 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     1801 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     1861 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     1921 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     1981 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     2041 cgggctgatc ccgtttttag caggctgcct catttttgca gtggtggcgc tatggctgca
     2101 ctggcgacat cgccgccagt aaaatttacg ttgccagatg cggcctgaaa cgccttatct
     2161 ggcctgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     2221 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     2281 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     2341 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     2401 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     2461 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     2521 ttactgactg cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca
     2581 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagtacca
     2641 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     2701 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     2761 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     2821 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     2881 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     2941 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     3001 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagtagac gttttccagc
     3061 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     3121 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     3181 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     3241 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     3301 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     3361 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     3421 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     3481 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     3541 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     3601 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     3661 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     3721 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     3781 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     3841 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     3901 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     3961 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     4021 acgtagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     4081 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     4141 agcgcggttt gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact
     4201 tccaccaaaa accaagtgac gaagataatc agcgcagtac cgagtattga ccagtattgc
     4261 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     4321 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     4381 ccggaagcgc cggtgatacg tgccacccag tcacccccac cggcttgcca gaagataacg
     4441 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     4501 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     4561 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     4621 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     4681 acataagcgg attaacacgc gcaggatcac aaatgcaggc attggtgagc aggaaaaaga
     4741 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     4801 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     4861 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     4921 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     4981 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     5041 gcgtctttcg tagttatggt gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     5101 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     5161 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     5221 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     5281 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     5341 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     5401 cggcgtactc taaccttgcg ttcgacctgc tggcagatgc actggcgaat gcgtccggca
     5461 agccttatac ccagttgttt gaagagcaga ttacccatcc attggggatg aaagacacca
     5521 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     5581 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     5641 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     5701 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     5761 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     5821 gtccgaggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     5881 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     5941 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     6001 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     6061 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     6121 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     6181 tcattccagt caatcattga taataatgtt gcgcgggttt gtttggctgg tagatgatat
     6241 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     6301 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     6361 agagagataa ctccttctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     6421 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     6481 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     6541 atgcccgtca aatccttgac gggcattatt tagattaaat taccagtatt tcttcggagt
     6601 gaagaatatt accaagtata tttaacaccc acgttcgcgg accagtcttg atctacgtca
     6661 ccaccaccga ggtagttagc atcggtatag gcgctgaagt tcttggtgaa gctgaactga
     6721 gtacccagac caacacgtac cgcagaccct tcagtaccgt tatcgatgga atcaccgttc
     6781 acatcgttat cgttgttaga gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga
     6841 gcctgatctt cgctgtaggt gaaggtataa cctgcatcta cacccagttc ataacgcatg
     6901 ctgtcgtaag gctgaccgtc aactttcatg tcgttgctca gctggtagtc atcaccagac
     6961 tggaacagac cagaaatgct gccgtaagga gtcacgtaac cagcatcacc cagtttgaag
     7021 tcgtaaccgg ttttcaaacc gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa
     7081 gtaccgttgc tcatggttgc agacaggtcg ttgttgaagt gagagtagct caagc
//
LOCUS       DAZSNM010000209         6963 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZSNM010000209 DAZSNM010000000
VERSION     DAZSNM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.209"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..208)
                     /gene="mxiI"
                     /locus_tag="ACHW42_003538"
     CDS             complement(<1..208)
                     /gene="mxiI"
                     /locus_tag="ACHW42_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFR8945198.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTT"
     gene            complement(221..472)
                     /gene="mxiH"
                     /locus_tag="ACHW42_003539"
     CDS             complement(221..472)
                     /gene="mxiH"
                     /locus_tag="ACHW42_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HFR8945199.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(480..1595)
                     /gene="mxiG"
                     /locus_tag="ACHW42_003540"
     CDS             complement(480..1595)
                     /gene="mxiG"
                     /locus_tag="ACHW42_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HFR8945200.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFFLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            complement(1604..2062)
                     /gene="ipgF"
                     /locus_tag="ACHW42_003541"
     CDS             complement(1604..2062)
                     /gene="ipgF"
                     /locus_tag="ACHW42_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFR8945201.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(2062..2424)
                     /gene="ipgE"
                     /locus_tag="ACHW42_003542"
     CDS             complement(2062..2424)
                     /gene="ipgE"
                     /locus_tag="ACHW42_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFR8945202.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(2434..4050)
                     /locus_tag="ACHW42_003543"
     CDS             complement(2434..4050)
                     /locus_tag="ACHW42_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HFR8945203.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            4364..5848
                     /gene="icsB"
                     /locus_tag="ACHW42_003544"
     CDS             4364..5848
                     /gene="icsB"
                     /locus_tag="ACHW42_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFR8945204.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            5861..6250
                     /gene="ipgA"
                     /locus_tag="ACHW42_003545"
     CDS             5861..6250
                     /gene="ipgA"
                     /locus_tag="ACHW42_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HFR8945205.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            6266..6892
                     /gene="ipgB1"
                     /locus_tag="ACHW42_003546"
     CDS             6266..6892
                     /gene="ipgB1"
                     /locus_tag="ACHW42_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HFR8945206.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
BASE COUNT         2286 a         1397 c          955 g         2325 t
ORIGIN      
        1 gcgtcgtctg taacttggcc aaagattctg ggtttaatga ttctggattg cttaccatct
       61 ccattatttg tgatacttga atatctgtat ccatcttaat atcactatat ttagccgaca
      121 cgacgtcttc cagacttgat atctcttgag attgaaaatc actggctttg ataatatcaa
      181 cctgattgac tggataaatg taattcatga atgctcccta ttatctgaag ttttgaataa
      241 ttgcagcatc aacatcctta atcactttca ctgtattgga ttgcgcgttc ctatataatg
      301 tatattcaga taatttactt tggtattcag ccagcaactg tggattcgaa ggatttttag
      361 ctaatttatc tagtgccaat gttagttcac cttgtaatgt ttgagttcca tcatcaaaag
      421 tttcagataa tgaactcaat gtccaatcat cattcggtac tgtaacactc attttatcct
      481 cacttatttt tatctaaaaa aaaccagtgt ttatcattca acattacata actgtctttg
      541 ctgttaagat atgatttacc tttaaaatca tcatctatca aaagtacaga aaactcaata
      601 tactgatctc catatatatt tttatgttca gaaatcagtc tattaatgag ctcaagcttt
      661 tcatctgtca gttcttctct gacagaatat gtaactttat tattttcaca aatctcttta
      721 tactggacat ttacctttgt gaaaatacct ttagcatccc cccttgcatt atgatcgcta
      781 agatagttga atttaatatt tttgatatac ggaaattcaa caatcagagc atttttcaat
      841 ttatcaagtt cggtatcttt tgaactattt ctttctttac ttaaaaacac cagtaattcc
      901 gctttatcac tcactaaatt caacacatac aaattgataa atgggaatcg ttgattgata
      961 tattgttgaa ttctatttat ttctttgttt gacaccagaa tatatctgca tttggtaaat
     1021 ccggttttat ttaaggctac agacgcccat accgcatcat tttgtgtttc ggcaagaata
     1081 taattgcact gtccagtgtt gactattcca tatctcttat catctataat ttcagcaatt
     1141 tcttttactt catctttctt taaagagagg aaaaaaatta taattaacac aacaataaca
     1201 gcaaagaaaa aaaacaccga gtggttctta tacattccgt ttagtatatg gcctctcgat
     1261 ttatcctccc tcatattttt aaggtgaaaa gaaatcccgt catattcaaa tgaattaaat
     1321 tgtgaattga tatcaatttg ctcacctttt tcatcactaa attgacagat attatctttg
     1381 cttatggtca aataaattat accatccgat ttactatctg ttacaggaat aatattttct
     1441 gggaaattgt cgtttccaaa ctcaagtgtt tctatcgtcc gtccgagaac aattaagtta
     1501 ttcccataat ataacggaaa ctcatccccg acaccgtttg tgagtttcac taataacctg
     1561 aatggtgcaa gatttgagtt ctttgcctca gacataaatc ctcttatatc cttcgattat
     1621 tctgcttgct ctctgctgct atccgcaaat agcgtctata gatatcctct gcgtacttta
     1681 atctttcttt tttctttttc ggagacgttc ctgcattata ggctccaaca gcctcccacc
     1741 ctcttccata catcatcata aactcattta ataactttgc tgcataatgt acagaaagac
     1801 atggatgact aattagcatt tcctcagaat atcccatttc tctaagtctt ttagaatgaa
     1861 agtcatttat ttgcattata ccataatctt tacttccatt gttattaaca ttgactgcag
     1921 atttattaaa tccggattct ttttccgcaa tcgcttttaa caaacttgat ggaatattgt
     1981 acctttcacc agccttatcc caacaatcag ctctccctaa atatgggata aaacacagca
     2041 aaataaatac gaaacgggac attaataccc cttcattctt cgcgcaaatt catccttcag
     2101 ccaacgcaca cgcgaaatat aatattctgt attaacatat acatcctcga attcatttat
     2161 cccggtaaga tctaatcgcg caattagatt tccgccttca tcatcagtag ctaagcatat
     2221 cttttctgag taatttaggc tcaacgcata tataagatca tttatatttt caggcaatgc
     2281 acaaaaaggg cataataaat ttattgaatc tccttctttt tcaatatata ttagcacatc
     2341 atcatcaagc atgattgctt gatcatcgat atcaattaaa gggataccca aggctcggca
     2401 aataacatct gctaaatctt ccatatattc ctcttataca aatgacgaat accctttcac
     2461 catattccat atttttgggt cccctattct ttcagaataa gatagctcta acgaagatag
     2521 cggcaatttt ttcatgactt tatttccggg cacaccagta ttcatctctt ggatttccat
     2581 attaccacta ttcattagaa tagtagaaaa taatcttttc tcttctgagg ataatttact
     2641 atttaattga gaaaactgac ctgtttcatg ttttcttatt atttctcttt taatttcagc
     2701 atcctgcata cctgttctgt ccttcccact cttacaattc cagcaaggta cagcacctat
     2761 agtataagcc aatagagtca ttctttgtga caatttatac ggctctccat tatcattttt
     2821 ttgtaacttt ttggttacaa tctcttttat ctggttagcc agatatatca catcattctt
     2881 acatggtgga tttttttcta ttgcttctgc agcccaacct ccaatcacac catttttgag
     2941 gaaattatcc cccaacaaag aacatattga ttcatcatta agcttgtcaa cattcctcca
     3001 gcctaagccc atttttagcg ctaattcatt tacaccaaag ttaaatgtca ccacttttag
     3061 attaacgctt acttctttca gaagaccatc actattccga attaataatt tcgttggctt
     3121 ccccctttta gagtttaacc cttttaatgc gttcacctga tcttttaaca tactctcctc
     3181 tcccccggtt aaactggtcg gcgtcagaag agaagtcgaa acaatcttta aatctactgt
     3241 tttaccagac aaagcctgtg ataataactc tggtctgcta tataatgctg cgcttactaa
     3301 ctcctcagct ttattacgag cagcaacagc tctttcagat gagttttttt tcaatccata
     3361 ggctgatata actccatgtc tgatacccga aaaaatttcc ttcccctcat catccaccac
     3421 ttttgaaagc cacatattcg cgatatgatc actctctctc gtagaagcac aacaaattcc
     3481 ttttccatta tactctttga caaatatatt tttattccct atcttcatat gagaggcagg
     3541 agttaattga aaaccataat ttttcccatg atgactaata ttcttgtcga ccggtcccca
     3601 attcttatta tttaaataat cagtaaatat ttgttttatt ttcgtttgca attccttttt
     3661 atttttaatg cctagctgtg ctcttatctc ttcacttatt actttattaa gctcattaat
     3721 atgtctttta ttaacttcac caagtttata ggtatctaat cgttccatca tggctttaat
     3781 ttttgacgta tcaccacctg ccaactttat tgcctcgcaa aaaacttcag ctctgtcacg
     3841 cgcaaacaaa aggctttttc ctgttaagct tgtttggttc tgtaaatata atcgattaag
     3901 cgcttcgatc cccttatttc tttctccatt tttaactctt tgatatgaaa tcacgcttac
     3961 acctttgtgc tttccggttt cttctgcgcc tttataggaa tctccgcttt gaaatgaaac
     4021 ctgatgcaat cccaaattag ttatgtgcat ttaattatcc tcagtcataa atggggcatt
     4081 gattgcagtt ttgtacctcg ttttacaaca aaaaagatac ttattcaaca gggttctctc
     4141 tttttctata acactccgag cctgatacat caggcacttt atcttgtggg atttcatgat
     4201 gaaacgagca ctacatatgc tcacgaggta catattatgt acagcaaaag aaaatttgat
     4261 tatgttaaca ggctcaagtt tctaaattta ttaattgaat atattatata ccaaatacat
     4321 tttacatccc cacaatcacc aagtaatgga gagttaataa agtatgatcc tcaaaattag
     4381 caatttcatt gacgcaagca atacaaaagg gcctatacgc gttgaagata cagagcatgg
     4441 acctatattg atagcacaga aattcaacct aaaggatctc tttttcagaa cattaagcac
     4501 tattaatgca aagataaatt cacagatatt aaatgaacaa ctaaaaaatt atcgactaga
     4561 gaatcaaaaa tccttattgc ttttcctcaa tacattagct agcgaaaaat ctgcagaaag
     4621 tgcgtttgcc gcctatgaag cagctaaaaa ctcaattcaa cactctttca caggtagaga
     4681 catcaaatta atgttgaata ccgcagagcg ttttcatggc atcggtacag ccaaaaatct
     4741 tgaaagacat ttagtttttc gttgctgggg aaatagaggc ataacccatt tagggcatac
     4801 tagtatcagt ataaaaaaca atttactaca ggaaccaact catacctatc tcagctggta
     4861 tcccggcggg aatgttacaa aagatacaga aataaactac ctttttgaaa aacgctcagg
     4921 ttacagtgtc gatacctata agcaagacaa actaaatatg atttcagacc aaacagccga
     4981 aagacttgat gcaggccaag aggttagaaa cttattaaat tcaaaacaag atcaaaataa
     5041 caacaaaaaa atattttttc ccagagctaa tcaaaaaaaa gacccctatg gttattgggg
     5101 ggtcagtgct gataaagtat acattccatt atcaggtgat aataaaacaa aggatggtaa
     5161 aatcagccat aacctttttg gccttgatga aacaaacatg tcaaaattta tatgcaaaaa
     5221 gaaagcagat gctttcagac aattggcaaa ttataaatta atcagtaaat ctgaaaactg
     5281 tgctggtatg gcacttaatg ttcttaaagc aggaaattct gaaatatact ttccactccc
     5341 tgacgttaaa ttggttgcta cacctaacga tgtatacgcg tatgcaaata aagttaggca
     5401 acgcattgaa agtctcaatc agtcttataa cgaaataatg aagtatattg aatctgattt
     5461 tgatctttcc agattgactc aattacgacg cagctatctt aaaagtttca ataaaattaa
     5521 ccttatccac acaccaaaaa cattcaagcc tttatcaatt tcactttaca aacatcctac
     5581 tgaaaatgta tcttcagaag actttgatgc ggtcatcaac gcttgccact catacttagt
     5641 aaaatcagca cccagcaata tgactcgtgt attaaacgaa ttaaaaaccg aagcaacaga
     5701 taaaaaagaa gaaatcatcg aaaaatcaat aaaaattatt gattattaca attctcttaa
     5761 atcgccagat cttggaacca aactttacat tcacgactta ttacagatca ataagctttt
     5821 attgaataac tctcattcta atatatagaa ggccatagaa atgtgtcgca aactatatga
     5881 taaactttat gaaataacag gtgcaaagct tgattttaac gataaaaatc aagcctttat
     5941 attgctcgaa gaacaaatac ctgtttgtat aacagataat gatgaataca tctttttgac
     6001 cggacttctt aatgaacatg aactttttac agaaaatatt attaatccag aacacatact
     6061 catccttaac tactctttga gtagagatta tggttcttct atctgtcttc tccctgatac
     6121 tcatcaatgt gttttgacca agaaacacta caaaaaatat ctttcacctg atgaactcat
     6181 tgaatctttg tatgaatttt tattttgtat taaactaacc attgcaaaca tcacttcaga
     6241 agtgaactaa catatagggg gtatcatgca aattctaaac aaaatacttc cacaggttga
     6301 attcgcgatt cctcgcccat catttgattc gttaagtcgc aataagcttg taaagaaaat
     6361 attatctgtt tttaacttaa aacaacgctt tcctcagaag aattttggat gcccggtgaa
     6421 tataaataaa atcagggaca gcgtcataga caaaataaaa gatagtaatt cggggaatca
     6481 actattttgt tggatgagcc aagagcgaac cacttatgtc tcttcaatga taaacagaag
     6541 tatagatgaa atggccatac acaatggagt tgttttgacc tcagataaca aaaaaaacat
     6601 atttgcagct attgaaaaaa aattcccgga tataaaactt gatgaaaaat cagcacaaac
     6661 atctatcagt catacagcac taaacgagat tgcctcatct ggcctcagag caaagatttt
     6721 aaaacgctat agcagtgaca tggatttgtt taacacacaa atgaaggatc ttacaaatct
     6781 agtatccagt tccgtctacg ataaaatatt taatgaatca acaaaagttt tacaaataga
     6841 aataagcgca gaggttctaa aggctgtata ccgtcaaagc aatacaaatt aaatctttta
     6901 tattaaatct tatacttatt aaagtaaagc agacaaaaag gagaccttat gtctttaaat
     6961 atc
//
LOCUS       DAZSNM010000210         6915 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZSNM010000210 DAZSNM010000000
VERSION     DAZSNM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.210"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..121)
                     /locus_tag="ACHW42_003547"
     CDS             complement(<1..121)
                     /locus_tag="ACHW42_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR8945207.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMP"
     gene            complement(109..1050)
                     /locus_tag="ACHW42_003548"
     CDS             complement(109..1050)
                     /locus_tag="ACHW42_003548"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR8945208.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(1474..3165)
                     /gene="fruA"
                     /locus_tag="ACHW42_003549"
     CDS             complement(1474..3165)
                     /gene="fruA"
                     /locus_tag="ACHW42_003549"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR8945209.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(3182..4120)
                     /gene="fruK"
                     /locus_tag="ACHW42_003550"
     CDS             complement(3182..4120)
                     /gene="fruK"
                     /locus_tag="ACHW42_003550"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR8945210.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(4120..5250)
                     /gene="fruB"
                     /locus_tag="ACHW42_003551"
     CDS             complement(4120..5250)
                     /gene="fruB"
                     /locus_tag="ACHW42_003551"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR8945211.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            5618..6796
                     /gene="setB"
                     /locus_tag="ACHW42_003552"
     CDS             5618..6796
                     /gene="setB"
                     /locus_tag="ACHW42_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR8945212.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFWVMTPTY
                     "
     gene            complement(6790..>6915)
                     /locus_tag="ACHW42_003553"
     CDS             complement(6790..>6915)
                     /locus_tag="ACHW42_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8945213.1"
                     /translation="RHNLNLRQHLARLGRKSLSFSKSVELYDKVIGHYLNIKHYQ"
BASE COUNT         1618 a         1916 c         1648 g         1733 t
ORIGIN      
        1 acggcatccc gtgagaaata atggtcgatt ccagcgccac aaccggtttt ttgtttttta
       61 aagcgtcctg cacttccgga gaaatttgta ataattcagg ggaaattttt aattcagaca
      121 ttctgcgttc tccactaacg atataacgtt ggcaatcgat aaatcggggt tattggtgta
      181 ttcacaggag agcgccattg acgaacatcc ctgtgcgaaa cgaacagatt cggcaaacgg
      241 cattccgtct acccaacacg aagcaagtcc cgccatcatg gcatcgcccg ctccggtaac
      301 attaataaca ttggttttga tcggcgcaga ccagccattt tcaccgctga tatcgctgta
      361 ataaacgccg tcgccgccca tgctcaatac cagtcggttc aggccatgtt gatggaacca
      421 ggcagcaact tttgccacat cgtcacgccc tgacagcgca atcccactca gggtttccgc
      481 ttcaaggcgg tttggcttca gagtgtggtt ctgatttaga cggtcgcgca ctttgacaca
      541 tttccacgct gaaaccggat cgacaaatac gggtacgttg gcggcattat ccagaatcca
      601 tgccagtgcc tcttcactga tattacagtc cgcgacaatg acctttgccc tctgaataaa
      661 ttcacggtgc tgtgcgagat attcagctgt aatagcgttg ctaatattca tgtcatttat
      721 agcaaccagc atttcaccgg tattatcgag taatgataaa taactcgacg tattttctcc
      781 cggcacaatc aggcatttat cgacataaac accagattga ttggtttgcg ttagcagcga
      841 ttgaccataa aaatcactgc ctacggcgct cagtagccag gctttgttac ccagcaacgc
      901 caggttttgt gcaatattgc gccctactcc accaggcgta aattttattt tacctggatt
      961 tgaatccgca taatttaatg attcatgtga atatccggcg acatcaatat tcgccgaacc
     1021 tataattacg acataatcct tttcgcgcat agcacgtcct ctggtaaaaa attaccaacc
     1081 ctgtcaatcc acaagaatat agatactgca aagacggttt aaacatgtgt tcatatttaa
     1141 acatgtgctc atagtatgca tgttttacgt aaagtaaatt accgccagag aggcaaaatg
     1201 ggcagatttg agcaggctca caaaatgcga ttaaatgcct gaattacaaa agcgaggaat
     1261 ttgtgacggg atgcacagaa caggaacact aaaaaagggc agaaaataat ctgcccttga
     1321 ggaaataacc ttagtgtttg atgaacgttt gaagtttcta ctgaaagata aaaccattta
     1381 aattcataaa attacattca caagctggtc attctctttg cgctcatcaa atgttacagg
     1441 gcaggaaatt tctgccctgt aacacacctt ttattacgct gctttcgcta ctgcgtccac
     1501 ttccggacgt ttcaggaagg cataggccaa acccgccacc agcgtaccgg caataattgc
     1561 taccaggtaa cccaataccg gcgtaatagc gccagggatc agcagaacaa acagaccacc
     1621 gtgcggtgcc atcagtttcg caccaatcgc cattgagatt gcgccagtca gcgccccacc
     1681 cacgatacag cacggcagca cacgcatcgg atcacgagca gcaaacggaa ttgcaccttc
     1741 cgaaatgaag cacagtccca ataccagagc ggctttgcca ccttcctgct gcgctttgtc
     1801 gaatttgcga cgcgccacca ttgttgccag gcccattgcc agcggtggca ccatacctgc
     1861 cgccataatc gccgccatcg ggccataggt ttgagtactc agcagaccca caccgaatgc
     1921 gtacgctgct ttgtttaccg gaccgcccat gtcagtacac atcatgccac cgaggatcgc
     1981 ccccagcaga accgcattcg cagtccccat ggtctgcagc cagtgagtca gcccttcgag
     2041 aatgccagca actggtttac cgatcaggta gatcatcgcc agaccgacca ccagactgga
     2101 aattagcgga atgatcagga tcggtttcag cgcctccata ctctgcggca gtttcagttg
     2161 cgtactgatt aacttcgcaa tgtaaccagc caggaagccc gcaataatac cgccaatgaa
     2221 gccagaaccg gtgctgaccg ccagcatacc gccaatcaga cccggcgtga ggcccggacg
     2281 atcggcaatg gagaaggcaa tataacctgc cagtaccggc accatcagcg caaaggctga
     2341 accaccacca atctgcatca gcgccgctgc caacgtaccc ggctctttaa acgcttcgat
     2401 accaaaagca aaagaaagcg cgatacacag accaccagca acgaccatcg gcagcatgta
     2461 agagacgccc gttagcaagt gacgataagc gcctgcactc tctttcttac cttcagtggt
     2521 cgccgtttga gctttgcccg ccggttcata cggcgttgct tcagcaaccg ctttatccag
     2581 ttcctgcgag gttttcttca gcgccagacc ggtagaggta cgatacatcg gtttaccagc
     2641 aaatttcgcc agatccactt cgatatctgc cgccacaatc accagatccg ctgcggctac
     2701 ttcctcggga gtgattgcat tacccgcgcc aacagaacca cgggtttcaa ctttcaccca
     2761 ccagccacgt tttttcgctt cggtttcaat ggcttcagcc gccataaagg tgtgtgctac
     2821 gccagtcggg caagcagtca ccgcaactac gcgtttcgga ccgctggcgg caaccggtgc
     2881 tgtcgcagtg accggcgcag tgtaaggttt cgcatggcct ttggcttcac tcaggaacag
     2941 ctcagggtgc gcaactgccc gggaaatatc acccagccag acatttttac cgttcagcgc
     3001 gctgtcattc gggatggaat caccgagaac aatcgccatt tctgcgtcgt tcggattgtc
     3061 gatgatttcc agttttgctt ttcgcgccgc cgcgcccagc agggtcttcg ccatataggc
     3121 gcgtgcctga ccgagattag cgtcaataat cagcagcgtt ttcattatgc ctctcctgct
     3181 gtcagttaaa aggttgtaag tcgacgcgcg ccatcattgc ggccaactgc ggacgatcgg
     3241 taatacccac attgctttga cttaccgcca gggctgcaac agctgtcgcc agacgcagtg
     3301 tgtgttcact ggattcacgc atcagcaagc cataaatcag gccaccaacc atagaatccc
     3361 ctgcgccaac ggtgcttacg acatcgactg acggtggttt agcgatccat tcgccggagg
     3421 cattaaccca aagcgcgcct tcggcaccca gtgaaataac aacatgcgca ataccttgtt
     3481 cacgtagcgc atgtgcagct tcaatcacat ctttcatttc aggcagttta cggcctgccc
     3541 agatttccaa ctcgcggcgg ttaggtttca ccagccacgg tgccgctttc aaacctgcta
     3601 ctaacgcttc acggctacta tcaaagataa tgcaaggaca ctgactacgc aggcgagtca
     3661 tccagtcggt gaacgcttcc gggctgacgc ctgacggtaa gcttccgctg acacagacca
     3721 tatcgaactg accgagccag ctcagagaat cagtcacaaa gcgttcccag tcggcggggg
     3781 tgacttcgaa acccgagaag ttgaagtcgg tcacttcgcc gtctttttcc gtcagcttaa
     3841 cgttaattcg ggtgcgcccc tgtacaacct ggaaacggtt ggcaatgccc agctcgctga
     3901 acagttgctg aaaaccatcc tgattgtctt tacccaggaa gccgccaacg gtgacatcaa
     3961 ttcccaggtc ttttaatact ttggccacgt tgatgccttt acccgccgca tgcagaccgg
     4021 tggttttcac caggttcact tcgccgcgtt caatttccgg gcagaaacca acaaggtcat
     4081 aagccggatt aagggtgatc gtagcaacac gtctgctcat tatgcgccct ccccaagacc
     4141 agcagcgata gcgtcgccaa tggctttcag cgcctgttca gcatctgcac cctgggcggt
     4201 aaagcgtagg cgatgacctt tcttaacgcc aagtgccaca actttcatca gactacgtcc
     4261 gtttgccggt ttgccggtgc catcaaggtt tgtcacggta atatcactgt taaattgttt
     4321 aatggtattg accagcatgg tacctggacg agcatgcagg ccgtgttcat tgcgcaccac
     4381 aaactccgcg cttaacacgt cgtcggtcgg cgcatcatcg ctggtcagca gcgccagcaa
     4441 cgttgccgca tccgctttca gcaagcggtc agctttattg tcgagcaaca aatcagcgag
     4501 acgcttaaga accgcgatgg gctgatcgtc attcatcgcc acactcacca gcatggctgc
     4561 cgtttcgccg tccacatcaa aagcatttgc cgcacggctt accgcaatcg cactacgcag
     4621 attgccttcg gcgctatcgc tcagccagat accctgtccg agattcagcg gttgttcatt
     4681 gatggctttg gtgacgaaag tggcgtcaac tgccctcgcc tctttcagac gcgcagcgtt
     4741 cagcgcctga agagtcagca gatcgctggc gacgatatcc agcgtcagca tttcgttgtc
     4801 gagcttcagc tgctcactct gcttttcgcc catcagtaat gcgcgaagtt cttctgctgt
     4861 tgttgctgac ttcagttgtt cagcaacgga atcatcgctc agtacgtggg tcagctggcg
     4921 tagcaggccc agatgtttat ccgagctggc agcaataccg attgccacgt acgctacctg
     4981 accgtcaccc caggtgacgc cttccgggaa ctgaaatacc tgaacgccgg ttttcagcac
     5041 ctgatcgcgg gtgtcggtag tgccgtgtgg aatagcaata ccattgccga ggaacgttga
     5101 ggtttgctgt tcgcgcgcca gcatgccatt gacgtagcct tctgctacat taccggcctg
     5161 caccagcgcc gcagcgacct ggcgaatcgc ctcttctttg tctccggcct tttcgcccgg
     5221 atggatgtcc tgtacggata actggaacat agttctcctc tcttgctgaa ttgaaacgat
     5281 tcagcctcta tgagaaaaaa agcgccaacc tagcttaggg ttaaagacaa gatcgcgctg
     5341 aaacgtttca agaaagcata atacttctgt ttcagcacgc aaggaaagcc gtaaagttat
     5401 gagcaaaagt ttgatgtgct gcacaatttt ttgtcatttt tcctaattgc tgatgggaaa
     5461 atccgttatc agcaatttca tttcagcagc gtcagcagcc aatcagcaac agtgaaatgg
     5521 cattttgatt attcgtggcg cttttgattt cgctgtctgt ttattttctg acaagcagcg
     5581 taaactccgc gtcttcctct tccagtgatc gaccagcatg cataactccc ccgcagtctc
     5641 cagcgcgaaa tcgtttgacc tgacctcgac ggcgttttta atcgttgcct ttctcaccgg
     5701 tattgcgggt gctctgcaaa ccccgacact cagtattttc cttaccgatg aagtacatgc
     5761 ccgtccggcg atggtgggat tcttctttac cggcagcgct gtcattggga ttctggtaag
     5821 tcagtttctc gctgggcgct ctgataagcg cggcgatcgc aaatcgctga ttgtcttttg
     5881 ctgcctgtta ggcgtgctgg cctgcaccct ttttgcttgg aatcgcaact actttgtttt
     5941 gctattcgtt ggcgtctttc ttagcagctt tggctcgacc gctaacccgc aaatgtttgc
     6001 ccttgcccgt gaacatgccg acaaaaccgg acgtgaggcg gtgatgttca gctctttttt
     6061 acgcgctcag gtttcactgg catgggtcat tggcccaccg ctggcttatg cgttagcgat
     6121 gggtttcagc tttacggtaa tgtatctgag cgcagcggta gcgtttattg tttgcggcgt
     6181 gatggtgtgg ctgtttttac cgtcgatgca aaaagagctt ccgctggcga ccggcacggt
     6241 tgaagcgccg cgccgtaacc gtcgcgatac gctactgctg tttgtcattt gtacattgat
     6301 gtggggctcg aacagcctgt acatcatcaa catgccgcta tttattatca acgaactgca
     6361 tcttcccgag aaactggccg gtgtgatgat ggggaccgcc gccgggctgg aaatcccgac
     6421 gatgttgatt gccggatatt tcgccaaacg tctgggtaag cgtttcttaa tgcgcgttgc
     6481 tgccgtgggt ggcgtctgtt tttacgcagg aatgctgatg gcgcattcac ctgccattct
     6541 gttgggcttg cagctgctaa atgctatttt tattggcatt ctgggcggca tcgggatgct
     6601 ctattttcag gatttgatgc ccggtcaggc aggttcagcc accacgctct ataccaacac
     6661 gtcgcgcgtg ggctggatca tcgcaggatc agtggcgggc atcgtcgccg agatctggaa
     6721 ttatcacgct gtgttctggt ttgcgatggt gatgattatc gccactctgt tttgggtaat
     6781 gactccaact tactgatagt gttttatgtt cagataatgc ccgatgacct tgtcatacag
     6841 ctccaccgat tttgagaacg acagtgactt ccgtcccagc cttgccagat gttgtctcag
     6901 attcagatta tgtcg
//
LOCUS       DAZSNM010000211         6785 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZSNM010000211 DAZSNM010000000
VERSION     DAZSNM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.211"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            230..2662
                     /gene="hsdR"
                     /locus_tag="ACHW42_003554"
     CDS             230..2662
                     /gene="hsdR"
                     /locus_tag="ACHW42_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFR8945214.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2729..4198
                     /locus_tag="ACHW42_003555"
     CDS             2729..4198
                     /locus_tag="ACHW42_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR8945215.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4198..5859
                     /locus_tag="ACHW42_003556"
     CDS             4198..5859
                     /locus_tag="ACHW42_003556"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR8945216.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6080..6421
                     /gene="symE"
                     /locus_tag="ACHW42_003557"
     CDS             6080..6421
                     /gene="symE"
                     /locus_tag="ACHW42_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFR8945217.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6468..>6785)
                     /locus_tag="ACHW42_003558"
     CDS             complement(6468..>6785)
                     /locus_tag="ACHW42_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HFR8945218.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2003 a         1610 c         1553 g         1619 t
ORIGIN      
        1 gaaagaaacg caactataag atttttatat attttgttag agttgatatg gttcctgtaa
       61 cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata acagagatat
      121 caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat agccaggcta
      181 ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca tggcggaact
      241 gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc cagccattct
      301 caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc gggacggtaa
      361 agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg acatcgtgct
      421 gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca aacatgaaat
      481 tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc cttttgtttt
      541 tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag gtgaatgcct
      601 tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc aaaaattctg
      661 cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact attacgacga
      721 tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa ccattgaagc
      781 cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag ggaaaaccta
      841 taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac gcattttgtt
      901 ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc agccatttgg
      961 tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa ttcacctcgc
     1021 gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac aagtcgcacc
     1081 agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat ccgaagacag
     1141 cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct taaccgccac
     1201 gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg tttacgtcta
     1261 ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg tccgtgttga
     1321 tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact taaacggcga
     1381 agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg tgatcgacga
     1441 acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca atccgatgga
     1501 taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc gcgccctggt
     1561 taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa tcaccggcga
     1621 tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac cgtacccggt
     1681 tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct gcaaactggt
     1741 agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg gtcgtggtac
     1801 acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta aaaaagccac
     1861 cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg ataccacacc
     1921 agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa tcagcgaaca
     1981 tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc aggttaccga
     2041 taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca agtttcacgt
     2101 caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg ccgacggtaa
     2161 actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag aatatgcctc
     2221 gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga tcattcacga
     2281 gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta aagatctcga
     2341 cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc gcaaagagcg
     2401 cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag cgcaagccgt
     2461 gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg aaagtattca
     2521 ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta ttaaaaccgg
     2581 atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg aaatctacca
     2641 attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca tcatgttgca
     2701 gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca aatcattaca
     2761 agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg gtcagctctc
     2821 ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac tggagcagga
     2881 taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg ccaacgcgca
     2941 gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc cggcgttgaa
     3001 aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc aggcgttcag
     3061 cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca acaagctgaa
     3121 cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt acgaacagat
     3181 ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac gcgccgtcac
     3241 tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg acccggcctg
     3301 cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat acgtgaagag
     3361 cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa aacagcttcc
     3421 gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg tacaaattcg
     3481 tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg atgtcattgt
     3541 taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact ttccggcaga
     3601 gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag tactggcgaa
     3661 aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag gtgttaaaac
     3721 caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc gtttaccgaa
     3781 tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta ccaaaggtca
     3841 gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga aaaactacag
     3901 caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt ggggtaacga
     3961 ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca gcattgatga
     4021 cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg aaaccgtcag
     4081 ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga tccagacgct
     4141 gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg ttaactaatg
     4201 agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt gcaaacccgt
     4261 tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa gaaattgcgt
     4321 gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc gaatgatgaa
     4381 ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt gaagcaaggc
     4441 aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc gtttgaattg
     4501 ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc tgagtcatct
     4561 agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat cgagcaaagc
     4621 caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt aaataatcca
     4681 gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga ttttgttgtc
     4741 ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt ctttttttgg
     4801 cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa agtattaaat
     4861 agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga aaaagttagc
     4921 tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct ggacgcacat
     4981 cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac cgcggaactg
     5041 gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac cgaagccagc
     5101 gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact tgtgccgcag
     5161 gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga aaaagctcaa
     5221 ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag cgatgaggaa
     5281 aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat agttaatatt
     5341 aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc acctgatatt
     5401 attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc aggagtcaaa
     5461 gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg cccgtcatta
     5521 tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta tccattagac
     5581 tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt tcttatgcaa
     5641 gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag tttttacaat
     5701 attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa gataaattct
     5761 attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa aacccaactg
     5821 catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc tcacaaaacc
     5881 aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt cacgctaatt
     5941 ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga tgatgtatcc
     6001 aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc acaatcacta
     6061 tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt tagaagcaga
     6121 agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt acccggatta
     6181 cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg gttttgccac
     6241 cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg cccaacccgc
     6301 cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc tgtcggcgcg
     6361 taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga aagtcgcctg
     6421 atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta taccaacgcg
     6481 ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg aagttgtgcg
     6541 aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt tgtcacatct
     6601 ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga caatcgcagt
     6661 cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc aatatatagc
     6721 ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg aagatgatcg
     6781 aataa
//
LOCUS       DAZSNM010000212         6539 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZSNM010000212 DAZSNM010000000
VERSION     DAZSNM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.212"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1013)
                     /gene="tdcD"
                     /locus_tag="ACHW42_003559"
     CDS             complement(<1..1013)
                     /gene="tdcD"
                     /locus_tag="ACHW42_003559"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR8945219.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLV"
     gene            complement(1039..2370)
                     /gene="tdcC"
                     /locus_tag="ACHW42_003560"
     CDS             complement(1039..2370)
                     /gene="tdcC"
                     /locus_tag="ACHW42_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR8945220.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(2392..3381)
                     /gene="tdcB"
                     /locus_tag="ACHW42_003561"
     CDS             complement(2392..3381)
                     /gene="tdcB"
                     /locus_tag="ACHW42_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR8945221.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(3504..4418)
                     /gene="tdcA"
                     /locus_tag="ACHW42_003562"
     CDS             complement(3504..4418)
                     /gene="tdcA"
                     /locus_tag="ACHW42_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR8945222.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCR"
     gene            4734..4952
                     /gene="tdcR"
                     /locus_tag="ACHW42_003563"
     CDS             4734..4952
                     /gene="tdcR"
                     /locus_tag="ACHW42_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFR8945223.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
     gene            5208..5748
                     /gene="yhaB"
                     /locus_tag="ACHW42_003564"
                     /pseudo
     CDS             5208..5748
                     /gene="yhaB"
                     /locus_tag="ACHW42_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
     gene            5770..6539
                     /locus_tag="ACHW42_003565"
                     /pseudo
     CDS             5770..6539
                     /locus_tag="ACHW42_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2004 a         1465 c         1320 g         1750 t
ORIGIN      
        1 accagacgac gaattaaact tgaattctct cctattccgc cggtgaatat aattccatcc
       61 agccgacgta atgaagctgc atgtccggca atatgacggg caattcggtg aacaaaggtt
      121 ttaattgcca gttgcgcgcg ttcgtgacct tcatgccagg ctttttccag aacacgtaaa
      181 tccgaagaaa gaccggaaat acctaataat cccgactctt tattcactac gcgttccagg
      241 tcacccaggc tctggttggt ttggctggcg acccaggaca tcgcaccaaa gtcgacatcg
      301 ccactgcggg tacccatcat caagccttcc agcggcgtca ttcccattga ggtgtcaaca
      361 ctctgaccgt tgcgaactgc gcagattgac gcgccattgc caagatgcgc cacaaccagg
      421 ccggagtcat cttccgccag attcagcagc gaatgtgcgc gctgggaaac atagcggtgc
      481 gacgtgccgt ggaaaccata acggcgtaca cctaactctt cataatattt ccacggcagg
      541 ccgtataaat aagcttccgg agccatcgtc tggtggaaac tggtatcaaa taccgccacc
      601 tgagttacgc ccggaaataa ttgctgcgcc gattcaatac cacttaaatt tgcgtaatta
      661 tgcaggggtg ccagtggaga aacgcgacgg atattatcaa tgacttcatc ggtaataatg
      721 gcggactcgg taaaaatact gccgccgtga gcgatgcggt ggccaattaa ggccacgctg
      781 tcatttaaat tccgtttttc cagttcaaat gcaattgcct tcaatgcacc ttcgtagctg
      841 tggtgagcca gcggtgctgg ctctccccca tttacggata agaatgcatt ttccgagtta
      901 ataccgtcgg caatacctga cattaatact tcacaatcgc tggcatcgag tacggaaaac
      961 ttaatcgaag acgaaccaca gttaataacc aaaacaaccg gaaattcatt catctctttt
     1021 ctcatcctga gttacggatt aaaacagttt gtatacgatg ttcaggatgg tcagcagacc
     1081 aatcacggta acaaacacgt tatccagacg accacggtat ttcgccagag acggcgcttt
     1141 acggatagca tacatcggca acaggcacag cagggatgcg ataatcggtg cgcccatggc
     1201 ttcaatcagg tcgaggatgt tcgggttggc gtaggcaaca acccaggtgg agcccatgat
     1261 gaagatcatg ctgagagtat tcagtttacc cagcgacact ttggttttgt cacctttata
     1321 accgaacttc agaatcagac cattcaagcc ttccagcgtc cccagatagt gaccgaagaa
     1381 agatttgaag atagccacga gtgcgatgat ggaagccgca tattccagtg taatcgcgaa
     1441 cgttgttttc gtaccggtca tggacgcaaa gtggttagcc agataagaaa gcactggaat
     1501 attctgcgct ttggcttccg ccatgttggc cggagacaga gtaaacaggc agctaaaggc
     1561 aaagaacatc accactgcaa ccatcagcat gctggcacga gaaatgattt gggaacattt
     1621 acgttcggtg aagtcgcgac cgaagtcttt ctcatactct tcacgtttag aaaccacgaa
     1681 ggaagagacg attggcgaga agttaaagga gaaaaccatg atggaaatcc ccagccagac
     1741 agtgatcagg ataccgtcat gaccggttaa cgacagcgaa ccgaggtcaa cctggtcgat
     1801 aactgcagag ttccagtaag ggatcagcga cagagaaatc agcaccaggc tggcgataaa
     1861 cggccatacc aggtagctca tcactttaac catcagatcc ttaccaaacc agatgacgaa
     1921 agccatcagc agcaacagga acagcgccac aaagccgcga ttcagcggtg caaatccgag
     1981 ctggttttcc cagaacgtca taaaggtatt ggtaatagta acgccataaa tccacagcag
     2041 tgggcaaatc gcgaagaagt acaggaacgt gataaccacg ccgccagttt taccaaaatg
     2101 ctcttccacc gtttccgtaa tgttgccgga agggttagag ccagaaagac acagacgcgc
     2161 cagcgcccgg tggcaataaa atgcgatggg gtatgccaat accaacatca gaagaatcgg
     2221 gatcagtccg ccaaaacctg cgcggatagg gaagaacagc accccggcgc cgattgccgt
     2281 accaaacaag cctaacgtcc atgtggtatc tgatttacgc caggacgatt gttttgtctg
     2341 gctggataca atgctatctg aagtactcat atcctatcct caacgaatta attaagcgtc
     2401 aacgaaaccg gtgatttgag agacgcgaga aagatcgata ttgccgccgg aaataatact
     2461 gacggttttt ctattttgaa tatattggtc taatttaccg cttaataatg cagcacatgc
     2521 cagagcgcct gcgccttcgg tgacgacttt attgcgctga attaaggcaa tcatactgtt
     2581 tctgatttcg tcttcgctga ccagcacgat gtcatcgact aattcacgaa cgatttcgta
     2641 agttaaatta cccgggcggg agacatcaca accatccgcc agggtgccgg tagttcggtg
     2701 cgtggttatt tctccggagt ggaaagaagc cgccatgccg tgaacgtttt ctgactgtac
     2761 gccaataaca cgaatggtcg ggttaataga tttaattgcc accgcaatac cagcaattaa
     2821 accaccacca ccaattggca caatcacgtt atcgacatca tagagatctt ccataatttc
     2881 cagaccaatc gttccctggc cagcaatcac tttcggatca tcgtaaggtg ggataaaaat
     2941 acgaccttcc atttcgacaa tttcgctcac tttagcgata gtgtcgttga agttatcacc
     3001 atgcagaacg acttctgcgg agtagtcgca cgttgccgct actttggatt ttggcgcacc
     3061 ttttggcatc accactttac cgtcgatacc cagcatcgcg caggagagcg aaaccccttg
     3121 cgcatggttg cccgcagaac aggccaccac gcctttgcgt ttttccgcat cggtcagtga
     3181 acttaattta ttaaatgcgc cacgaatttt aaatgaaccc gtacgctgca tattttcaaa
     3241 cttcaggaat atttcacctt tgcaacgttc actaaaatag ttggagcgag gcatgcctgt
     3301 tttataaatt cgcccagcca gtcgttgttt cgcttcaata atatcatcaa tagcaaccgg
     3361 cagatcgtat gtaatatgca ttataaaacc tcttcgcctg aattaaatat gtaggtaacc
     3421 gtaaccgaca cctgcaaaac agacaggtgg attatttatg tttaaataaa aacaaacaac
     3481 taaccaactt ctattaattg ccttcatcta cacccattat aagatgaata ctctttggct
     3541 aattccacca aaaccgatgc tgctttttta atacgataat ttttcgacca taccgcggca
     3601 tattgtgcca caggtaatgt ttcttcaacc ggaatagtaa taaattgatt agaaccaaaa
     3661 ggtgacgtca tatcacaagg aattacagtt aagaaatcag cattgagaac aagattataa
     3721 attgtcacga ctgagtcggt tttaacgatg ttttcaatac tgatgccatt tctttgtaac
     3781 gtagtaagca gttcgctgta gtaccccata ttagtttgtg gcaacaccca ctgttcgttc
     3841 ttcaacgact ccagcgtggt ggtgccggtg catgttcggg acttactggc taccagcaca
     3901 aactcggact cgaacagcgg ctcaacatgt aaatcctgaa gcttcatttc tgcacttaac
     3961 gtaccaatcg caaaatccag tcgaccgtcg cggattgccg gtaagaacga agacagttgc
     4021 gcttcataca tagaaacctg cgctttcggg aacacctctt tgaacttgtt gatcatccct
     4081 gacataaaag taaaaccaat caatgaagga aaaccaaatg agacttccac caccgcttca
     4141 gaagacatac cgcttatctc attaaccata tttttcattt cacgggtaat ggattcggaa
     4201 cgggagagta acaattgacc ggcaggtgtt aatgttacac cggtattttt ccgcaccact
     4261 aattccacac caaaataatc ttcaatatcg ttaatgattt tactgacggc cggttgagtt
     4321 aaccctaatt cttttgcagc cgagccgata gagccacttc taatgacttc ctgaaagact
     4381 accaggtgct gcgttttcgg aagaagaata gtgctcataa cgacctacgt taattacctc
     4441 attgacggca tgaagtgtat caaaatgaaa taaacaggat atgtgcgacc actcacaaat
     4501 taactttcat tactttccaa ggtatcgtta ttacaattaa acatctttaa atatcaacaa
     4561 gttaaagcat aaaaatcagc ataaaccctg atttttatca aaaaaaaatg acggggataa
     4621 ccatatttta tggcgataac atcattcgtt atgtggttaa tacaaaaaag gggctgagac
     4681 catatttcaa gcaattaccg gataattacc aggcaaaatt tgagttaaat ttaatgagca
     4741 agttctcaaa ttttattata aataaaccat tttcagcgat aaatactgcg gcacgtcaca
     4801 ttttttcacg ctatttgttg gagaacaaac atttatttta tcaatatttt aaaatttcga
     4861 atacaggtat tgatcatctc gaacaattga ttaacgtcaa ctttttctct tctgacagga
     4921 cgtcattttg tgaatgcaat cgttttccat aaattcttct cccctcatag gcgacgaata
     4981 gcattttgtg ttgaggatca caaaacgaat aattgctggt cgccgcgata aggtcagacg
     5041 aagtcaacaa gggggatttt cactgtgctt tacatcagcg taggacacag aatgttgctt
     5101 cgtttactgt aacgccgggt aaatgagcgt tttttgatag tgcgtaagtg tcgtgcgagg
     5161 aaaataagca tctattattg tttgtcatga cgacaaggca ggcatcaatg aaaggcttcc
     5221 caattgcaca tatttttcac ccttcaatcc cgccaatgca cgcagtggtt aacaatcaca
     5281 atagaaatat tgattattgg acggtaaaaa gaaagtttgc agaaattgtc tccaccaatg
     5341 acgttaataa aatttacagt ataagtaatg aactccggag agtattatct gcaattactg
     5401 cattgaattt ctatcaaggc gatgttcctt ctgtcatgat tccgaatcca accggaaaat
     5461 atgagtccat tcatcataga tatttctaca ggagaacatg atgattatat catacaaaca
     5521 ttagatgtag gcacttttgc accttttggt gaacaatgta cttgctcagc cgtcaataaa
     5581 aaagagctgg aatgtattaa ggagacgatt tctaagtatt gcgcaaagtt cacccaaaaa
     5641 gaagccatct taacaccccc taggcacttt aataaaacct gcattacttc agattgttgg
     5701 caaattctct ttttttcacc cgatcatttt aataatgatt tttattgatt atttataaag
     5761 gataaaataa tgttccctgt ttcatcaatt ggtaatgata ttagtagtga tttagtccgt
     5821 cgaaaaatga atgaccttcc tgaaagcccc ataggaaata acctcgaagc actagcacca
     5881 ggtatagaaa aactaaaaca gacctctatt caaatggtca ctttacttaa cgcgttacaa
     5941 ccaagtggaa aatgcattat cactggtgat tttcaaaaag aattagcgta cttacaaaat
     6001 gtaattcttt ataatgactc gtctcttcgt ctggattttt taggttataa cacccaaatt
     6061 attcaacgat cggacaatac ctgcgaactt accattaatg aaccgttaaa aaactaggaa
     6121 atatctacag gcaatatcaa tgttaattgc ccattaaaag atatttacaa tgaaatcagg
     6181 aggttgaacg taatctttag ttgtgggact ggagatatag ttgatctatc ctctctggac
     6241 ttacgtaatg tcgatttaga ttattatgat ttcacagata aacatatggc taatactatt
     6301 ctaaatcatt ttaaattgga ttcaacaaat tttactaatg ccaacatgtt tcaggttaat
     6361 tttgttagtt caacacaaaa cgccacaatc tcctgggatt atttactaaa aataacgcct
     6421 gttttaataa gcattagcga tatgtattct gaagaaaaaa tcaagtttgt cgaaagttgt
     6481 ttaaatgagc ttggagacat taccgaagaa caattaaaaa ttatgagatt tgcaattat
//
LOCUS       DAZSNM010000213         6398 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZSNM010000213 DAZSNM010000000
VERSION     DAZSNM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.213"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..88
                     /gene="yjbL"
                     /locus_tag="ACHW42_003566"
     CDS             <1..88
                     /gene="yjbL"
                     /locus_tag="ACHW42_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR8945224.1"
                     /translation="RDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            112..819
                     /locus_tag="ACHW42_003567"
     CDS             112..819
                     /locus_tag="ACHW42_003567"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR8945225.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            1182..2219
                     /gene="dusA"
                     /locus_tag="ACHW42_003568"
     CDS             1182..2219
                     /gene="dusA"
                     /locus_tag="ACHW42_003568"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR8945226.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2353..2595
                     /gene="pspG"
                     /locus_tag="ACHW42_003569"
     CDS             2353..2595
                     /gene="pspG"
                     /locus_tag="ACHW42_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR8945227.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(2761..3744)
                     /locus_tag="ACHW42_003570"
     CDS             complement(2761..3744)
                     /locus_tag="ACHW42_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR8945228.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3827..5242
                     /gene="dnaB"
                     /locus_tag="ACHW42_003571"
     CDS             3827..5242
                     /gene="dnaB"
                     /locus_tag="ACHW42_003571"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR8945229.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5295..6374
                     /gene="alr"
                     /locus_tag="ACHW42_003572"
     CDS             5295..6374
                     /gene="alr"
                     /locus_tag="ACHW42_003572"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR8945230.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
BASE COUNT         1596 a         1492 c         1658 g         1652 t
ORIGIN      
        1 tcgggatata attattgcaa aaatagattt gtttattcaa cgaatcacgc ataatttatg
       61 gcatgcacgt aaaaaaatgt gtttttaatt gagcacataa atgatctcaa aatgtgggtc
      121 aataagtata ctgacgattg cactgatgag gatttaaacg atcgtgactt tattgcatca
      181 gttgttgacc gggctatttt tcatttcgcg attaatagta tatgtaatcc tggggataat
      241 aaagatgcaa tgcccattga acaatgtact tttgatgtag aaactaagaa tggccttccc
      301 tccacggttc agctatttta tgaggaatct aaggataatg aacctttagc gaatatacat
      361 tttcaagcaa taggttctgg ttttttaacg tttgttaatg cctgccagga acatgatgac
      421 aacagcttaa aattatttgc ttcgctgtta atttcacttt catattctag tgcctacgca
      481 gatttatcag aaacagtgta tattaatgaa aataatgaga gctacctgaa agctcagttt
      541 gaaaaattat atcaacgtga tatgaagaag tacctgggag agatgaagcg tctggcagat
      601 gggggagaaa tgaattttga tggctatctg gataagatgt cacatctggt gaatgaagga
      661 acgctcgatc ctgatatttt aagcaaaatg cgagatgctg caccacaatt aattagcttc
      721 gcgaagtcgt ttgacccaac ctcaaaggaa gagattaaaa tacttacaga cacttctaaa
      781 ttaatttatg acttgttcgg ggttaaatcg gagaaataat atgtgaagtt cttcgatagt
      841 atggaaggca ttatataaaa ggacccaata tttattgggt tcttttttct ctatcaatgc
      901 tattagcagg gagatatatc accagagttt aatgtgtgat tttttattta tcgtcgaacc
      961 tggattgttt atcattggcc ttaacaaagt taacggctaa taagattatt tccatcactt
     1021 cgtgagagct tcatgccttg agaggatctc aattttcttt tgcaatgaga caggcgcttc
     1081 ctgttgttat ggtatagtac cccgctattg agcctcctga acagtgaagc tgaataacat
     1141 aaccccatga tatatcgata aaataatctc tacatttgaa aatgcacggt aattctgaaa
     1201 tgcaaaaaat caaccaaacc agcgcaatgc ctgaaaaaac tgacgttcac tggagtggtc
     1261 ggtttagtgt tgcaccaatg ctcgactgga cggacagaca ttgccgctat ttcttgcgtc
     1321 tgctttcccg caatacgttg ctttataccg aaatggtgac cacaggggcg ataattcacg
     1381 gtaaaggtga ttatctggcg tacagtgaag aagaacatcc ggtagcgttg caactgggcg
     1441 gtagcgatcc ggcggcgctg gcgcagtgtg caaagctggc agaagcgcgc ggatatgatg
     1501 agatcaacct gaatgtcggc tgcccgtctg accgggtgca gaacggcatg tttggtgcgt
     1561 gtctgatggg taatgcgcag ctggttgccg actgcgtgaa agcgatgcgc gatgtggtgt
     1621 cgattccggt gacggtgaaa acgcgtattg gcatcgacga ccaggacagc tatgaatttc
     1681 tctgcgattt catcaatacc gtttccggca aaggcgagtg tgagatgttc atcatccacg
     1741 cacgtaaagc ctggctttcg gggttaagcc cgaaagaaaa ccgtgaaatc ccgccgctcg
     1801 attatccgcg tgtgtatcaa ctgaagcgtg acttcccgca tctgacaatg tcgattaacg
     1861 gtggtatcaa gtcgctggaa gaagctaaag cgcatttgca acatatggat ggcgtgatgg
     1921 tcgggcgcga ggcgtatcag aatccgggta ttctggcggc ggtagaccgg gagatctttg
     1981 gttcctcgga taccgatgcc gatccggtgg cagtagtgcg tgccatgtat ccgtacattg
     2041 agcatgaact cagccagggg acgtatctcg gccatattat ccggcatatg ctgggtttgt
     2101 tccagggtat tcctggcgcg cggcagtggc ggcgttattt aagtgaaaat gcccataaag
     2161 cgggtgctga cattaacgtg ctggaacacg cgctcaaact ggtggcggat aagcgttaac
     2221 ttttcaccaa aaagtagtca aattcaccac gccctgcgca ccgtcgcggg gcgttttgct
     2281 gttaaatcaa tagattattt ttggcatgat tcttgtaatg ccagcaagag atttcatatt
     2341 tgggagagca tcatgctgga actacttttt gtgattggct tttttgtcat gctgatggtc
     2401 accggcgttt cgttgctggg cattatcgcc gcgctggttg tggcgacggc cattatgttc
     2461 ctcggcggta tgctggcatt gatgattaag ttgctgccgt ggttactact ggcgattgcg
     2521 gtggtgtggg ttattaaggc gattaaagca ccaaaaatgc cgaaatatca gcgttatgac
     2581 cgctggcgtt actaagggat tgtgcggatg atcacaacct aaggttttat ccttagaaca
     2641 aaataggaat tgataatcaa atctgtcact attgcgcctc taacagattc atcgtgctgt
     2701 accctacata cagccgaact ataaaaagaa agggcttccc aggtggaagc cctatttctt
     2761 ttatggaatc agcaggctgg aaccttgcgt cgcccggctt tccagaatct catgcgcacg
     2821 ctgcgcatcc ttcagcggat atttctgctg ctcggcgaca tcgaccttaa tcacaccgct
     2881 ggcaatcaaa gagaacagtt cattactggc ctcggttaat tcctcccgcg tggtgatata
     2941 gccttgcagg gaagggcgtg tcacatacaa cgagcctttt tgattgagaa tgcctaagtt
     3001 cacaccggta accgcacctg atgagttgcc aaaactgacc attaagccgc ggcgttgcag
     3061 gcaatccagc gaccgttccc aggtgtctct gcccacggaa tcgtacacta cgcgcacttt
     3121 cttaccgccg gtgatctctt tcaatcgctc gaccagattc tcttcacgat agttaataac
     3181 ctgccacgcg ccggctttta gcgcgctctg cgctttttgc gcggttccta cggtgccgat
     3241 gagtttcgcg cccagggctt ttgcccactg gcaggcaatt aagccaatgc cgccagccgc
     3301 tgcgtggaac aggaactgtt catcgggttt aatttcatag gttttgcgca gcagataata
     3361 aaccgttaag cctttcagga aggatgccgc agcttgctca aaagaaattg ccgcaggcag
     3421 aatcgccgct ttatccgcat taatgttatg cacagagctg taagcgccta acgccgactg
     3481 cgcatagact acatgatcgc cagccttaat atgctttaca ccactgccga ctttactcac
     3541 gatgcctgcc gcttcggtac ctaatccgct gggtagcgat ggcggcgggt aaaggccgct
     3601 gcggatatac gtgtcgataa aattgatgcc gatggcttta ttttcgacct ggatttcatt
     3661 ctccgccgga tcggcaggag tgaactctac ggcttgaagt acttccgggc caccgtgctt
     3721 gtgaaattca attcgtgttg ccatgtgtcc tccagaacgt atcgtcaggg tctgcttcat
     3781 atggtaaagt ttcgacccat tctttatttc ggtaactcca ttcactatgg caggaaataa
     3841 acccttcaac aaacagcagg ctgaaccccg cgaacgcgat ccacaagttg ccgggctgaa
     3901 agtgcctccg cactcgatcg aagcggagca gtcggtgttg ggcggtttaa tgctggataa
     3961 cgaacgctgg gatgatgtag ccgagcgtgt ggtagcagac gatttttaca cccgcccaca
     4021 ccgtcatatc tttactgaaa tggcgcgttt gcaggaaagc ggtagtccta tcgatctgat
     4081 tacccttgcg gaatcgttgg aacgccaggg gcaactcgat agcgtcggtg gttttgctta
     4141 tctggcagag ctgtcaaaaa atacgccaag tgcggcgaac atcagtgctt atgcggacat
     4201 cgtgcgtgaa cgtgccgttg tccgtgagat gatctcggtt gcgaatgaga ttgctgaagc
     4261 cggttttgat ccgcaggggc gtaccagcga agatctgctg gaccttgctg aatcccgcgt
     4321 ctttaaaatt gccgaaagtc gtgcaaacaa agacgaaggg ccgaagaaca tcgccgatgt
     4381 gctcgacgca accgtggcgc gtattgagca gttgtttcag cagccacacg atggcgttac
     4441 cggggtaaac accggttatg acgatctcaa caaaaaaacc gctggcttgc agccgtcgga
     4501 tttgatcatc gtcgccgcgc gtccgtcgat gggtaaaaca acatttgcga tgaacctcgt
     4561 cgaaaacgcg gcaatgttgc aggataaacc agtacttatc ttctcgctgg agatgccatc
     4621 agaacagatc atgatgcgtt ctctggcgtc gctgtcgcgc gttgaccaga ctaaaatccg
     4681 taccgggcag ctcgatgatg aagactgggc gcgcatttcc ggcaccatgg gtattttgct
     4741 cgaaaaacgc aatatctata tcgatgactc ttccggcctg acgccaacgg aagtgcgttc
     4801 ccgcgcacgc cgtattgccc gtgaacacgg cggcatcggg cttatcatga tcgactacct
     4861 gcaactgatg cgcgtaccgg cgctttccga taaccgtacg ctggaaattg cagaaatctc
     4921 ccgctcgctg aaagcactgg cgaaagaact gaacgtgccg gtggtggcgc tgtcccagtt
     4981 gaaccgttct ctggaacaac gtgccgacaa acgtccggtc aactccgacc tgcgtgaatc
     5041 tggctctatc gagcaggatg cggacttgat catgtttatc tatcgtgatg aggtgtatca
     5101 cgaaaacagt gatttaaaag gcatcgcgga aattattatc ggtaaacaac gtaacggccc
     5161 aatcgggacg gtacgcctga cctttaacgg tcaatggtcg cgcttcgaca actatgcggg
     5221 gccgcagtac gacgacgaat aataattatt ttatgaatta ggtaattaaa gcaaacaatt
     5281 atcaaggaac acaaatgcaa gcggcaactg ttgtgattaa ccgccgcgct ctgcgacaca
     5341 acctgcaacg tcttcgtgaa ctggcacctg ccagtaaaat ggttgcggtg gtgaaagcga
     5401 acgcttatgg tcacggtctt cttgagaccg cgcgaacgct ccccgatgct gacgcctttg
     5461 gcgtagcccg tctcgaagaa gctctgcgac tgcgtgcggg gggaatcacc aaacctgtac
     5521 tgttactcga aggctttttt gatgccagag atctgccgac gatttctgcg caacattttc
     5581 ataccgccgt gcataacgaa gaacagctgg ctgcgctgga agaggctagc ttggacgagc
     5641 cggttaccgt ctggatgaaa ctcgataccg gtatgcaccg tctgggcgta aggccggaac
     5701 aggctgaggc gttttatcat cgcctgaccc agtgcaaaaa cgttcgtcag ccggtgaata
     5761 tcgtcagcca ttttgcgcgc gcggatgaac caaaatgtgg cgcaaccgag aaacaactcg
     5821 ctatctttaa taccttttgc gaaggcaaac ctggtcaacg ttccattgcc gcgtcgggtg
     5881 gcattctgct gtggccacag tcgcattttg actgggtgcg cccgggcatc attctttatg
     5941 gcgtctcgcc gctggaagat cgctccaccg gtgccgattt tggctgtcag ccagtgatgt
     6001 cactaacctc cagcctgatt gccgtgcgtg agcataaagc cggagagcct gttggttatg
     6061 gtggaacctg ggtaagcgaa cgtgataccc gtcttggcgt agtcgcgatg ggctatggcg
     6121 atggttatcc gcgcgccgcg ccgtccggta cgccagtgct ggtgaacggt cgcgaagtac
     6181 cgattgtcgg gcgcgtggcg atggatatga tctgcgtaga cttaggtcca caggcgcagg
     6241 acaaagccgg ggatccggtc attttatggg gcgaaggttt gcccgtagaa cgtatcgctg
     6301 aaatgacgaa agtaagcgct tacgaactta ttacgcgcct gacttcaagg gtcgcgatga
     6361 aatacgtgga ttaatcgttc tgtaatattt gattgtct
//
LOCUS       DAZSNM010000214         6310 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZSNM010000214 DAZSNM010000000
VERSION     DAZSNM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.214"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..314
                     /locus_tag="ACHW42_003573"
     CDS             <1..314
                     /locus_tag="ACHW42_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8945231.1"
                     /translation="VAMIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKT
                     HDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNV
                     R"
     gene            complement(390..854)
                     /gene="nanQ"
                     /locus_tag="ACHW42_003574"
     CDS             complement(390..854)
                     /gene="nanQ"
                     /locus_tag="ACHW42_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR8945232.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(851..1726)
                     /gene="nanK"
                     /locus_tag="ACHW42_003575"
     CDS             complement(851..1726)
                     /gene="nanK"
                     /locus_tag="ACHW42_003575"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR8945233.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEEL"
     gene            complement(1723..2412)
                     /locus_tag="ACHW42_003576"
     CDS             complement(1723..2412)
                     /locus_tag="ACHW42_003576"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR8945234.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2460..3950)
                     /gene="nanT"
                     /locus_tag="ACHW42_003577"
     CDS             complement(2460..3950)
                     /gene="nanT"
                     /locus_tag="ACHW42_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR8945235.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPM
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4060..4953)
                     /gene="nanA"
                     /locus_tag="ACHW42_003578"
     CDS             complement(4060..4953)
                     /gene="nanA"
                     /locus_tag="ACHW42_003578"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR8945236.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5075..5866)
                     /gene="nanR"
                     /locus_tag="ACHW42_003579"
     CDS             complement(5075..5866)
                     /gene="nanR"
                     /locus_tag="ACHW42_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR8945237.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1598 a         1703 c         1565 g         1444 t
ORIGIN      
        1 aagttgccat gatttacatt gaacagcttg aattaatcca taaaagtggt gatgtgttgt
       61 atccagtaaa aataaccaga aaatcatcag gaaaaactgc cttccacctt gttccattcg
      121 gcttaaataa gactcacgac cttctagagg tggaagatgc ttcagaagca attcggctgg
      181 tgattgatga acgtcattcc attcgctgca gtacacttac agcaactatt acgaataaaa
      241 agggcaagcg aattaagcgg acaggtatat acagtatcaa gggtgttaac ataaaagaat
      301 acaacgtcag gtaatggcac agagcggtcc gtcagttcag gtttggctct gtgccaaaga
      361 tgggtcaggt cacacctgat ctaataaatt taagccatta aatcagcctt aaccttcacc
      421 accacctttt taatctcacc cggctcgcca acaacgcatc ccggcttatg cggctctcct
      481 ggcataaaca cggcgaacat tcccggtttc agaatgatgg tttgctcgtt ttcaatggcg
      541 ctacaaagct ggtaatcatc ttcatggtgg aactcttcac attggcgcgc agtgcctgcc
      601 atgccaaaca gaatccgttc ctcaccgttt aataacagct ggatatcaat gtattgctcg
      661 tgcaattccg cttttttctc gacgggcgat tgagtgttaa acgtcatgac attcataaaa
      721 atattgtcgc cctgtaattc gtaacgaccc ggcgcttttt cttgcggtct ggcagctaag
      781 gccagcgtta acgcgtcctg taacgcagga tgtaacccag cagacggtaa tgactgtact
      841 tcacccatca tcataattct tctccctggg ccaacagcgc agccccaagt aaacctgcat
      901 catggcggta atgcgccgcc agtaaatcaa catgaaatgc cgctggctcc tgcgccagat
      961 acatttccac caacgccaga tacccttctg ccagaccaac gctgccacca accaccacgc
     1021 actggcaatc agttgtggct ttaatatcag cgatcagcct tgcaagcgta cgtgcggagc
     1081 gatgaatcag ttgctgcgcc tgctcatcgc cctgcccggc gcgtgtgaaa atagttctcg
     1141 catccgcgcc agccagctcg ccctgcgctg ccgccgcaat gccgcgacca gaagcaattg
     1201 cttccacgca acctgtgcgt ccacagccgc agactgggcc gtgtggatcg gcaagcgtat
     1261 gcccgatgtg ccccgccaga ccgccagggc cggtaagcag tttgccgccg ctcactacac
     1321 cgccgccgac gccagtggaa acggtgataa agaccatatc ggttatatcg ccttccagcg
     1381 cctgatactc tgcccatgct gcggcctgcg cgtcgttaat ggcaatggtc ggcaaattgg
     1441 taagttgttc cagcgttttg actaacggaa agtgtagcaa tccaccgaga ttatgcggat
     1501 tcagcgccag caaactgccg tcgcgaataa ttccggttga agcgatggca acccgctgcg
     1561 catgagcttg caacggagag actaatgcgg ataaggcatc acgcaaggct tctggtgtct
     1621 ggctggctgg cgtaggaagt tcacgacgat cgcggatctg cccgtcagcg ccaatcagcg
     1681 cggcggcaag tttagtaccg ccgatatcaa tcgccagtat ggtcatagca ccgccttttt
     1741 catcgctgtg ttgtaccact gacaaatgtg ttcaagacgc gtaatggcag aaccgaccgt
     1801 caccgcccac gcgccgtggc gcatcgcatc tgccgcctga gcaggcgtgt tgtaacgccc
     1861 ttcggcaatc acccgacatc cggcttcgct caacgttttc accagcgcca gatccggctc
     1921 ttctggcgtt tcaggcgtgg tatagccaga aagcgtagtg ccaataattt cggctcccag
     1981 cttttggcat gccaggccgt cttccggcgt tgagcagtcg gtcatcgcca gtaaaccgtg
     2041 atgatggata cgtgccagca gcgtttcaac aggcaccgga cgcgggcggt cggtgccgtc
     2101 aatggcgata atgtccgcgc ccgcctgcgc cagcgcatcg acatcttcaa tataggccgt
     2161 gatgcgtacc ggagaatcct ccagatcgcg tttcacaatt ccaataatcg gcacgctcac
     2221 caccgcacgc gtggcttgca gatttgccac accttcgatg cgaatggcaa ccgcgcccgc
     2281 ctgctctgcc gctaatgcca tggctgcgac gatttcgggt ttatcgagcg ggctgtcagg
     2341 aaccggctgg caggagacaa tcaggccacc gttagcagcg attttttgat ccagttgtgc
     2401 aagtaacgac atacatcttc ccttagcgaa aggcccggta catagaccgg gcaacaggat
     2461 taacttttgg ttttgactaa atcgtttttg gcgctgccaa atggcacggc accgctaaat
     2521 ggtttgccgt cgatagcgtc atgagtacgc aatgcttccg ggcgcagcca acgctgaacg
     2581 cgggaaggca tatccagacc aatcagcagg atcaccacaa atgtcaggct gaacgagagc
     2641 gatgccagcg cagtacccag atccagacgt tgagcgatca acgcgccgat gattggggcc
     2701 agtgcgccac ccaatgcgcc aacgttgtag gtaaagccca ggcccgctgc acgctggtcg
     2761 gtatcgaaat aaccgccaat cagtttcggt aagatcccgg cgatcccttg tccaagcatt
     2821 tgctggaaga acagtaacag accgagcacc cagacgtttg cgccgccaat cgcaaatacc
     2881 ggaataatca gcagctgcga ggccagcagg ctacaaacgt acgctttgcg ggttcccagc
     2941 cagtcaccga ggaagccacc tacgcagcat cccacggccg cgccaaagcc actaaagaac
     3001 agcacattgg cgacagtatg cgggttataa gccagatcgg ttttcagata cgttggcagc
     3061 agcgcctgaa tcggccatga gtagaggaaa gcaaacaaca cgaccaccat cagcattacg
     3121 cccattggcc agcgtttacc cgtactttgc accataaagc tgataaagat tgcggcgcat
     3181 aacagcccga gaacagcgac gattgcagca ttttgcaggt taccggcgaa gcagaaccac
     3241 agcgcagtag ccgccgccag tgtcattacg atattggcaa tgcgatgttc accacggtag
     3301 agaatatcca ccattgtgcg tactggtgct ttacctgcgt gtttctcttt ccagtcttcc
     3361 gcttccggga tgtttttacg cagccagaga gcaaagatga ttggcaaaat gccgataaag
     3421 aacagcgcac gccaacccca gaccggaacc accaggctat agacctgagc ggcaacgacg
     3481 gcccccacag agaagcctga aatcaaaaaa ccactggctt tgttacgcag atgttttggc
     3541 cagctttcaa tgacataggt ggcgctggaa ccgtattcac ccgccatccc catgccgatg
     3601 accagacgag cgataaacat ggtgatgtag cctggcgcaa agccgcaggc cagcgtcccg
     3661 gccgagaaga gaacgatgct ggtgaccatt gccagacgac gcccgtagcg gtcacccata
     3721 gcgccgagca tcaggccgcc gaaccagcga gagataaagg ctgcggagat cagacttgcc
     3781 gcctgcaccg tcgtcagccc gaattcaccc tgtacttcgg tgagtaccag ggcgattaaa
     3841 acgaaatcaa aaccgtcaag cagatatccc aaccaggcag cggaaaatgc gcgccattgt
     3901 gcacggttga gatggcgata ccacgggata ttctgggttg tagtactcat tgtgagtctc
     3961 ccgcggtggg cgatgcccac acgctttggt atgaaaattg tagggtacag atgcgtttat
     4021 ttcccctcac ccggtagggg cgagcgaggg gaaaacaact cacccgcgct cttgcatcaa
     4081 ctgctgggcc agcgccttca gttctggcag atatttttca tctaccggtc caaacggttt
     4141 gcggcacagc ggcacagaaa cgacatccat ataatgcagg acagttttca ggccgcggaa
     4201 tacgcccgtt ttgatcagta aatcaatgac tttgttgcac tcagtttgca gtttctgcgc
     4261 ggtctggata tcgccttctt tcagcgcctt cacgatcccc tgatatcgcc agcccatgat
     4321 gttgtaagta ctgccgatac caccatcagc gcctgccagc aagccggaag cgaagatttc
     4381 gtcgtaaccg ttatagagca ccagatcagg atgttcacga cggatctgtt ccatctgata
     4441 gagatcgcca gaagtctgtt tcagcgcacc gacgccaggc aacgtaacca gggtgttgat
     4501 ctgatccagg gtcagtttta ccccactcag ggctggaatg ttgtacacca ccatcggcaa
     4561 accatccgct gaatcaataa ttgcccgata gtgatcgcag tgttcttcaa agctgaaagg
     4621 atagtagaac ggcgtgacgg cggagacggc atcgaagcca taacgtttag ccgatgccgc
     4681 aagttgttgg ctttcggcgg tgctgacgca accgacgtgg gcgatgagtt taatcttacc
     4741 tttcgcctct tcggcgacga tttccagtac ctgttcacgc tcggaaaggc tttgtacaaa
     4801 ggcttcgccg gtcgaaccac ccacgtataa accgtcgatg ccctgctgaa tattgaactg
     4861 aaccaggcgg cgcagactcg ctttatccag tgcttgttgt tgatcaaaag gagtcaggag
     4921 tgcagccatt acgccacgta aattcgttgc cataaatacc tctgaagtga tgcttgtctg
     4981 ataaacgata tacctttata cctgttatac cagatcaatt aagcaacacc ccatacagaa
     5041 agcttataat gcgatctgct tcactaaagt ggcattattt ctttttgttg gtggtctgac
     5101 cgaaagcgtg ccaggtagca gagacgctgt tgagatgcga ttgcaacgca cgatcggctt
     5161 cgtcaggatc atgacggcgg atcgcatcaa cgatcgcaat atgctgttga taactaacgt
     5221 tgttatgttc gtgcagtgcc tgatcggtaa ccgttgggcg tgcggcaata agccagtcga
     5281 gcagggcaac gtggatcgcc atgaagattg ggttaccggg gatctccgcc agcacgcggt
     5341 ggaaatcaac gtctgaacga atgaatgccg cgttgttatc cagcgactga ctgttgattt
     5401 ccagtgcttt tgccagcaaa tcgatttgct catcggtggc gtgttcagcc gcatagcgca
     5461 ccaggctgga ttcaaagaac agacgtaatt gttcgaaatg ggcaatccca ccaggatgag
     5521 aaaggaaatc tttcgccatg ccggaaagct cgccgatgat agtgtccgca gaaggtcgag
     5581 agacacgagc gcgttcgccg ttgtttattt gcaccagacc tttgcgtttt aacgctgcca
     5641 gcgcttcacg cacagaagga cgcccgacgt taaagaacgc catcagttcg cgttcagacg
     5701 gtaattgttc accttcgcca aattcacgac ggcggatcat ctgttccagc tcttcttcca
     5761 ccatttcgga gagtttttta cgcgccagcg ggcggctacg caagttgcga ccaattgcag
     5821 gtgaagaatc ttcggtttgc gaatcaaatg cgttcataag gcccattctg taaggtcagt
     5881 gtgattaaca tcatcagtga catcctatca caggattgaa agtaggggaa aatggcaggt
     5941 ttttctcttt gtgcctcatc attaccataa ttaacggaat aattaactat tgcgaaaaat
     6001 taatgtaacg cagataaaca catcccgatt gaattattca taagactatt cacaagcatt
     6061 atgaatatta tgaatgtgtt cttacaaaat aatcataatc gcatattttt taatgaaaaa
     6121 tcacctcacc tacaattaaa aacacgacat ccgcaccata aatagacttg caaaaaatat
     6181 aacatcattg ttttcaatct gccatttatg ggattgaccg ttttcttttg acacggagtt
     6241 caacaatggg taatgcttcc aacttactga tttagtgtat gatggtgatt ttaaggtgct
     6301 tgcgtggctt
//
LOCUS       DAZSNM010000215         6255 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZSNM010000215 DAZSNM010000000
VERSION     DAZSNM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.215"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHW42_003580"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHW42_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR8945238.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="ACHW42_003581"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="ACHW42_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR8945239.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHW42_003582"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHW42_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="ACHW42_003583"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="ACHW42_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1661 a         1455 c         1470 g         1669 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgttaa ttaacaaatg atatcttcta tatattttta
     6181 ttgtttatag atggctattt cggtaaaaat gcattttcag aatatcaacc agcggtaaat
     6241 cgaagtaaag ggagt
//
LOCUS       DAZSNM010000216         6115 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZSNM010000216 DAZSNM010000000
VERSION     DAZSNM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.216"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(191..334)
                     /gene="ypaB"
                     /locus_tag="ACHW42_003584"
     CDS             complement(191..334)
                     /gene="ypaB"
                     /locus_tag="ACHW42_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR8945240.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            462..2747
                     /gene="nrdA"
                     /locus_tag="ACHW42_003585"
     CDS             462..2747
                     /gene="nrdA"
                     /locus_tag="ACHW42_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR8945241.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2981..4111
                     /gene="nrdB"
                     /locus_tag="ACHW42_003586"
     CDS             2981..4111
                     /gene="nrdB"
                     /locus_tag="ACHW42_003586"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR8945242.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4111..4365
                     /gene="yfaE"
                     /locus_tag="ACHW42_003587"
     CDS             4111..4365
                     /gene="yfaE"
                     /locus_tag="ACHW42_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR8945243.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4419..5069)
                     /gene="inaA"
                     /locus_tag="ACHW42_003588"
     CDS             complement(4419..5069)
                     /gene="inaA"
                     /locus_tag="ACHW42_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFR8945244.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5149..5253)
                     /locus_tag="ACHW42_003589"
                     /pseudo
     CDS             complement(5149..5253)
                     /locus_tag="ACHW42_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5248..5490
                     /locus_tag="ACHW42_003590"
                     /pseudo
     CDS             5248..5490
                     /locus_tag="ACHW42_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5532..>6115)
                     /gene="glpQ"
                     /locus_tag="ACHW42_003591"
     CDS             complement(5532..>6115)
                     /gene="glpQ"
                     /locus_tag="ACHW42_003591"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR8945245.1"
                     /translation="IGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQC
                     FDADELKRIKNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAM
                     KQVAEYADGIGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTP
                     DVNQLYDALYNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT         1540 a         1621 c         1444 g         1510 t
ORIGIN      
        1 gcttcgcatt taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac
       61 attcgcttat atattgacca caactgatac atcagattat gtgatgactc gtgcttagat
      121 caatttttgc aatcattagc aaaaagatta ataaaccatc tatatcaatt tatccaacct
      181 attctgccgt tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg
      241 actttcccgg acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt
      301 tatccacaaa gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc
      361 aaacataccc cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg
      421 aaactattgc ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg
      481 tgacaaagcg cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg
      541 attgggcggc agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca
      601 ttcagtttta tgacggtatc aagacctctg acatccacga aactattatc aaggctgccg
      661 cagacctgat ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct
      721 tccacctgcg taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg
      781 tgaaaatggt cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag
      841 agttcaagca gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg
      901 ccgttaagca gctggaaggc aaatatctag tacagaaccg cgtgaccggc gaaatctacg
      961 aaagcgccca gttcctttat attcttgttg ccgcgtgcct gttctcgaac tacccgcgtg
     1021 aaacgcgcct gcaatacgtg aagcgttttt atgacgcggt ttccacattt aaaatttcgc
     1081 tgccgacgcc aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac
     1141 tgatcgagtg cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgtgaaat
     1201 atgtttccca gcgtgccggg atcggcatca acgccggacg tattcgtgcg ctgggcagcc
     1261 cgattcgcgg tggcgaagcg ttccataccg gctgtatccc gttctacaaa catttccaga
     1321 cagcggtgaa atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc
     1381 cgatgtggca tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggggttgaag
     1441 gcaaccgcgt gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgcc
     1501 tgctgaaagg tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg
     1561 cgttcttcgc cgatcaggaa gagtttgaac gcctgtatac caaatatgag aaagacgaca
     1621 gcatccgcaa gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg
     1681 cgtctaccgg tcgtatctat attcagaacg ttgaccactg taatacccat agtccgtttg
     1741 atccgaccat cgcgccagtt cgccagtcta acctgtgcct agaaattgcc ctgccgacca
     1801 aaccgctgaa cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt
     1861 tcaacctggg cgcaattaat agcctggatg aactggaaga gctggcgatt ctggcggttc
     1921 gtgcacttga cgcgctactg gattatcagg attacccgat cccggccgcc aaacgtggag
     1981 cgatggggcg tcgtacgctg ggtattggtg tgattaactt cgcttactat ctggcgaagc
     2041 acggtaaacg ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcga
     2101 ttcagtatta cctgctgaaa gcgtctaatg agctggctaa agagcaaggc gcgtgcccgt
     2161 ggtttaacga aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc
     2221 tggatgccat cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca
     2281 aaacgcacgg cctgcgtaac tccacgcttt ctgccctgat gccgtccgag acctcttcgc
     2341 agatctctaa cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt
     2401 cgaaagacgg tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg
     2461 agctgctgtg ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga
     2521 aatttatcga tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag
     2581 gaaaagtgcc gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca
     2641 aaacgctgta ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac
     2701 cgtcaatcca ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg
     2761 gatgcggcgt aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc
     2821 cagcgtcgca tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc
     2881 tcggatttgt aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa
     2941 aatgccttat ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc
     3001 acagacgaaa aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc
     3061 tcgctacgat cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt
     3121 cttctggcgt ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc
     3181 ggagcacgaa aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat
     3241 tcagggtcgt agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac
     3301 ctgggtcgaa acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat
     3361 tcgtaatatc gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat
     3421 ccagaaacgt gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta
     3481 ctggcacctt ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct
     3541 gcgcgagctg aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat
     3601 ccgcttctac gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg
     3661 taacgccaag attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca
     3721 gcatatgctg aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga
     3781 agaatgtaag caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg
     3841 ggcggattat ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca
     3901 gtacgttgaa tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca
     3961 gacgcgctcc aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt
     4021 tgctccgcag gaagtggaag tcagttctta tctggtcggg cagattgacg cggaagtgga
     4081 caccgacgat ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc
     4141 acacaactgc tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat
     4201 gtggcggttg agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc
     4261 gcaggtcagg tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg
     4321 ccctgttgtt gccgggcaaa aggcgatatt gaaatcgagt tgtgatcggg tactagcgcc
     4381 agatacgacg cttacgcgta ttatctggcc tacaacgatt acatggcgta gtaatacgct
     4441 ttcacctgct cccagtccgc tttagggatc ggctccagat atttttccag ctggcggaag
     4501 tcatgattaa tcgctttatc gcgacgcaag cggcgacgac ttttctccag atcaagaaaa
     4561 ccagcttctg ccttaccttc tgttttcaca tagatatggc gaacatagca acagccatgc
     4621 tgacgattaa cgctatgcat tttcttaaac gccagtgcca ctgctttcaa catggcttgc
     4681 cgtacttcgt cagaataagg cgatactgca tgccgggcat accagtcagc aatgctgatg
     4741 aaccccgcca tatcttcagt caccagcaac gcgcgccatt caccctcaat tttcaccgct
     4801 tcaccaaaaa cgatcttcgg cacgatgaca ccggcccgtt ccagttcttt aattaccgca
     4861 acctcacgga caatcgttgg tcggccgaac ggataacgta ctgaatgaaa cagatgatgc
     4921 gtcatgcgct ttacatacag ctttttgccg ttgcgctcga cgcattgcac cccgctcata
     4981 ccattacggc gatagttagg ctcttcaacc cagtcgccct ctgttgccca ccagtgatta
     5041 aattcgtcgt actttgctga aactgccata ccctatcgcc tgtcattttt attaacgaca
     5101 atgactatag gtggttacct gaggaaaatc ttaatgaaac gtgtcgtatt aatgaatttc
     5161 ttttgccgct acaggtgtga caccgctttt gcccttcatt tgcagcgcat agattccacc
     5221 agccactacc actaatagtg cgcccagcag ataaaatgtg aatttaatca gtcggataca
     5281 gcgatgaatt ttattcgaca gggattaggc atcgccttac aaccagagtt aacgctgaaa
     5341 agcattgcag gtgaattgtg ttccgttcct cacgaaccaa ccttctatcg acagatttcg
     5401 ttgctgacta aagaaaagcc ggtagaaggc agtccactgt ttttactaca aatgtgcatg
     5461 gaacaattag tggcgattgg aaaaatttga tatgagacag gatggcgcat gaacgccatc
     5521 ctgcagtaac attactcttt attaagaaat tttactgcct tatcagggaa atcagtaaac
     5581 agcccattta cacccgcttt gttatacaga gcatcatata actgattcac atcaggagtg
     5641 tattcaggca gtttatctga ccgcacggta taaggatgca ctaccagttt attctgctgg
     5701 gcatcttgca ccatgccagt gagtttgata ttacccggct gcgatgtctc ctcaatcaac
     5761 atatggtaat ccggaccaat accatctgca tattccgcca cctgtttcat ggctcccggc
     5821 ttaaacatcc agtcgtagtt gtaattaacc cagcttccat ccggctgttt ctgctgcgtt
     5881 tcattccagt cggtataggc aatcagctgt accagattga gctccatgcc cattttgggt
     5941 tccagctcat tcttaatacg cttcagctca tcagcatcaa aacattgcaa ataaacttta
     6001 tcgtctttac cggtgtaacc atatttcttc agcacttcca gcgtttttgc cgcaatatcc
     6061 ttcccttcct gatgatggaa ccacggcgct ttgatttctg gatagatacc gatat
//
LOCUS       DAZSNM010000217         6058 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZSNM010000217 DAZSNM010000000
VERSION     DAZSNM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.217"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..143)
                     /locus_tag="ACHW42_003592"
     CDS             complement(<1..143)
                     /locus_tag="ACHW42_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HFR8945246.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="ACHW42_003593"
     CDS             379..651
                     /locus_tag="ACHW42_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFR8945247.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHW42_003594"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHW42_003594"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR8945248.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1731
                     /locus_tag="ACHW42_003595"
     CDS             1111..1731
                     /locus_tag="ACHW42_003595"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR8945249.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="ACHW42_003596"
                     /pseudo
     CDS             complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="ACHW42_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3114..3767)
                     /locus_tag="ACHW42_003597"
     CDS             complement(3114..3767)
                     /locus_tag="ACHW42_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR8945250.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3767..5238)
                     /locus_tag="ACHW42_003598"
                     /pseudo
     CDS             complement(3767..5238)
                     /locus_tag="ACHW42_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5211..6058)
                     /locus_tag="ACHW42_003599"
                     /pseudo
     CDS             complement(5211..6058)
                     /locus_tag="ACHW42_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1621 c         1599 g         1411 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggatc ttcggtgcgt tgtgctggat gacgaccttt
     1681 acgcgggtgt ggagtgggta tctgacgctg aagtcgctcc agcgtcagta attgcaatgt
     1741 tatttgctac tgcccgggcc gcgttcaccg gttgcaaccg gattttttgg atcgctgctc
     1801 cattcgtacc agccgccgtc atacacggaa acattcttcc aacccatggc gcgtgcgtac
     1861 ataaaggttt cggacgcgcg ccagccggtg ccgcagtaga atgaaacttg ctgatctggt
     1921 ttgatattcc atgctttcca catagcggta atatcatcgg cgctacgcat ggtgccatcc
     1981 gggttataga aatcttccat atgcgtcgag tcgctaccag cgtgtcccca acgtgctccg
     2041 gctatttcac cttttggttt aatatagctg taaccgctgg tcgtaccgat aaattctggc
     2101 cacgaacgaa tgctcaccag cgatgcatcc tggcgatgca gcagtccacg ggcctgttcc
     2161 atatcgagca tcaactgcgg ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc
     2221 actttcggtg gcgttcctcg ctcaacaggc agtcccgcgt cggaccaggt ttgccagccg
     2281 ccatccagca gacgcacatc tttcacgcct gcgtagagca taatttgcgc cacacgcgct
     2341 ccagcgtata cgtcacgccc gtacagaatg accgtggtgt catggcgaat gccgtgtttt
     2401 gccagcatcg cttttagttg ttcatcagaa actttgttcc acagcggttc gctttccact
     2461 tcgttggtat cgatgtagtc agcgccagga atatggctga taaggtaaag cttaggagcg
     2521 ccctaggccg cttcaatgac tttccagtca ccgacaggtt tcgccgtaac ctctttacct
     2581 tgttgcaggt catgcagcca ttgcggataa accagctgct caaagtgcgg cagtttttgt
     2641 agacgggaag gttcgcttag cgcgtcactc agaatggaga tatgcgtaaa acctgctttt
     2701 tgcagtcgcg ttttgacggc gtcaacatct ttgccattac cgtacagcgc caccggggca
     2761 tcggctttca ggttatgttg cttgatccac gcgttgagct gttcggtgct cattttgtcc
     2821 agccagctag cagagaggtt taaggcggca ggttcatgac cagaagggcc atttaaggtt
     2881 tgtggccagc cgttataaaa cgcgctgggg cgagtatcta tcgctttgcc attttgttgc
     2941 tgaagttggt caagtgtaag aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc
     3001 agccctaaag ccattgccag cgcggtcatt tgagaaacac gtttcatcga aaatcccgac
     3061 attgtagttt tgagtgcgcc attctgaact gacgcattga gaaaaacttt gcgttatcgc
     3121 agtttgttgt aattttctga ccactgcgcc atatccagaa cagaactatc agcaggaaca
     3181 tcctggagat cgtgcgttac ctgaacgacg gggatcgcca gggcgcgaac ttcgctgaac
     3241 acccactggc gaaaattatc gcgcagagcc acatcaagac ggctgaatgg ctcatccagg
     3301 agtaacgctt ttggctgggc gagaagggcg cgtagtagag caacgcgcgc tcgctgaccg
     3361 ccagacaaag tggcaggatc ctgatggaaa gctccgtcaa ggcctgaacg ctcaagtgca
     3421 tcattcacgg cattacgtcg ggcattcccc ttaagtgtcg ccggtagcgc cagcagtaaa
     3481 ttttgcccga cgctgaactg gtcaaataac agtgcatcct gaaaaagaat gccaatctga
     3541 cgctgtgcgg tgggtagcat gtcaatccgt tgctcattga gccatagctc acctgtacaa
     3601 gaaaactgtc cggccagtgc accaatcatc catgaaaaca gagtggattt tccacagcca
     3661 gacggcccca ttaacgtgac gatgtcacct ttatcaaccg taaagttaac gtttgtcagc
     3721 aagcggcttt ctggtaaacg tagcgaaaca tttttcacgc agagcattag cggattcctt
     3781 gtctgacata acctacccat tttgcgacta acgcggtcag ggcaaaaata ataagcggta
     3841 acaacagttg ccataacgcc tgggcggcga gaataccgtt gctgccgccg ctgcttaatg
     3901 ccaccgcctc agtggtgagc gtcggaaaac gccccgcgcc cagccacagc gtcggcatat
     3961 actgcgcaat actgactgaa aatcccaccg cgaaggcaat cagcacaggg cgcaacatga
     4021 gtgggcattt cacgtaaaag aagattttgg cccgcgacca gcccagtgtt tgcgcaatca
     4081 aaattaaccg tgaatcaatg cgctgccagg cgggttgcag gataaacagc atccacggca
     4141 ttacccacag cagatgcccc cagaccaccg ccgtccagct tccatccagg ttcagccata
     4201 gcgccagcgt gtactggccc gccaccagtg gcagagcggg caatagaatg ggcaaccata
     4261 gccacaactg gcgacgctgt gccccccatt ccagccacag cagtaacagg agcaaagcga
     4321 tgaatgttgc taccagcccc attgtcagac tgttgatgag tgcttcacta ttgatcgtcg
     4381 actgatccgc gaggatcgcc agcagaacca cacacagcac accggttaag ggtaaaaaac
     4441 tcgccagcgt agtgcctggt aataaaggtg tggcaggctt gcgaatgcca tctacgcggg
     4501 gaatagtgcg ccgccagctg cgccaaaacg taggcggcga gtaacagcat cagcagcaga
     4561 ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct gagttaacca ctgccagcta
     4621 attactgcca gcgtcggtgg attacctggc ccaagaataa tcgccacatc cacgaccgaa
     4681 agcgaccagg caacaatcgc cagcatcgcc attgccagcg gctgtagccc agcgaatcca
     4741 gcacaatgac ctgctgcaac agccgttttt cgctcaatac tgccactaag atccacagca
     4801 gaaatgcgct ttctttcact gcaagggtga ggcccagacc gatccaaatc ggtccattgg
     4861 cggagtgaaa tacgggaaat agtcatatag cagccctccg tcagcaaaga gcagcagggc
     4921 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg cgcacatacg
     4981 ctgccatttc ggcccaggcc atagcgccac aatcaccagc agggcaatca acaatgcccc
     5041 gaccgccgcg atggttgtcg acaccagcgt tgccagtaat gcctgcggta actgtggatc
     5101 ggcaaataac gcctgccagc gtgtcaacga caacgcaggc gatatcagcg tgagagctgc
     5161 cgggatcagc ggtgcataaa tcaccgccac catcgcccac agcaaaaaga ttaatgcgta
     5221 ccgtaacggc gtaaccattc ttgttccagt gcattcaccc atcctgcgtg cggttcagcc
     5281 agtaccggcg gcagatcctg cggcattctt gattgcaata tttcgcgctg cccgtcaggc
     5341 agtttttgcg gatcgagaac agaaggatcg ccccagacaa cgggatctgc tttacgcagt
     5401 tgcgcatcgg gtgagagcag gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5461 ttggctggaa tggtgacgaa atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg
     5521 taactgcttg ccggcaaatc gccgctggcg attttttgct gcgcatgtgc ggggttaaag
     5581 gtcagcgaca ggcgcaatgt gccggctttc agcagagcat ccatccgcgc gggcgaaggc
     5641 gggaaatcct ttccttcgcg ccacaaatac ggatgcagtg catcaagata ttgctacaag
     5701 ggcgcagtga cacgggcgaa agtcgcatcg tccggcgctt cttttaatgc ggcgggatct
     5761 ggcgtcagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc
     5821 ggataagtaa ccgtgccggg attggcttta gcaaactcca gtaaggcttg cggcgtttgt
     5881 ggtggctgtg gcgtaacatc gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc
     5941 gattccgcac cttgtgtcgg cactgaaaaa tctccccgca ccggcagctg tgtgtcgaca
     6001 tagcgccagt tgggcagagt ctccgcccag cccgtttgta ataaattggc ctctttta
//
LOCUS       DAZSNM010000218         6017 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZSNM010000218 DAZSNM010000000
VERSION     DAZSNM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.218"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="ACHW42_003600"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="ACHW42_003600"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR8945251.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="ACHW42_003601"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="ACHW42_003601"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR8945252.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="ACHW42_003602"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="ACHW42_003602"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR8945253.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="ACHW42_003603"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="ACHW42_003603"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR8945254.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="ACHW42_003604"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="ACHW42_003604"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR8945255.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
BASE COUNT         1490 a         1397 c         1575 g         1555 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgc
//
LOCUS       DAZSNM010000219         5816 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZSNM010000219 DAZSNM010000000
VERSION     DAZSNM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.219"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            194..1102
                     /locus_tag="ACHW42_003605"
     CDS             194..1102
                     /locus_tag="ACHW42_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFR8945256.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1148..3139)
                     /gene="tkt"
                     /locus_tag="ACHW42_003606"
     CDS             complement(1148..3139)
                     /gene="tkt"
                     /locus_tag="ACHW42_003606"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR8945257.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3417..4175
                     /gene="loiP"
                     /locus_tag="ACHW42_003607"
                     /gene_synonym="yggG"
     CDS             3417..4175
                     /gene="loiP"
                     /locus_tag="ACHW42_003607"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR8945258.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4381..5301)
                     /gene="speB"
                     /locus_tag="ACHW42_003608"
     CDS             complement(4381..5301)
                     /gene="speB"
                     /locus_tag="ACHW42_003608"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR8945259.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5437..>5816)
                     /locus_tag="ACHW42_003609"
     CDS             complement(5437..>5816)
                     /locus_tag="ACHW42_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945260.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1400 a         1540 c         1524 g         1352 t
ORIGIN      
        1 caaaatcaac attttgtttg ttattgtatt tgtgatgcat atctctattt atttattgaa
       61 tgttcgaaaa catacttttc taatgcaatg ttttttcgct ataccccttt atagattctg
      121 aattctcacc tgtcggctgt tttctcctca tctcatgtga catattccca tactttactc
      181 aaggaagatt gtcatgaaag cacctgtttc attaacctca tttcgcccac aaaaatctct
      241 tgctatcgcc attggcgtgc ttgccgtggt tgtgctgcca tttctctcgt attacaccgt
      301 taatgaaggt gaacgcggca ttttgctgcg ctacggaaaa atcgtaaaag ttgccgaacc
      361 ggggctgggt tttaagatcc cttttatgga aagcgtggag aaaatttcca cgcgtaatca
      421 ggctgtggtc tatcagggat tacaggccta cagccgcgac cagcaaccgg cacaaatgac
      481 agtatcagtc agcttccaca ttaaaccttc agaagcaggc gcggtttata ccacctacaa
      541 cactattgaa gcattaaaag atcgtctaat tgtccgtcag ttgccaactc agctggagaa
      601 cattttcggc caatataccg ccatcagcgc cgtacaggat cgtacaaaat tggttcagga
      661 tctgcaaaat gccatgcgca aagccgttgt tgggccagtg gtaattgacg gggtccagat
      721 tgaaaatatt gatttttctg acgcttatga aaagtcgatt gaaaaccgaa tgaaagcaga
      781 agtcgccatt gccacgcgta agcaaaatct ggaaacagag aaaatccagg cgcaaatagc
      841 ggtaactcag gcccaggcag aagcagacag taaactggcg gcagccaaag cagaagcgga
      901 aactattcgc gtaagaggcg cggcagaggc tgaaactatt cgtttaaaaa gtgcggctga
      961 agcagaagca atccgcctgc gcggtgaagc tctgcgggat aatcccggac ttgtagccct
     1021 caccactgcc gaacgttggg acggtaaact gcccgatacc atgatccccg gcagcacagt
     1081 tccgtttatc tctactaagt aaaaaaaggg ccgctttcgc ggtccttctc acaaaacgtc
     1141 ttactgatta cagcagcgct tttgctttcg caacaacgtt atcaacagtg aagccgaact
     1201 cttcaaacag cagctctgcc ggagcagatt caccgaaggt ggtcatgccg acgatagcgc
     1261 cgttcaggcc aacatacttg taccagtagt cagcaatacc cgcttctacc gcaacgcgtg
     1321 cagtaaccgc tttcggcagt acggattcac ggtaagccgc atcctgcttg tcaaatgcgt
     1381 cggtagacgg catggacacc acgcgcgctt tcacgccttc ggcagtcagt ttttcgtagg
     1441 cagcaaccgc cagttcaact tcagaaccgg tggcgatgaa gatcagttcc ggctgaccag
     1501 cacagtcttt cagcacataa ccaccgcgcg cgatgtttgc cagctgctct tcagtacgtt
     1561 cctgctgcgc caggttctga cgggagagga taagcgcagt cgggccgtcc tgacgctcaa
     1621 cgccgtattt ccacgcgacc gcggattcaa cctggtcaca cggacgccat gtagacatgt
     1681 tcggggtcac gcgcagagaa gcgacctgct ctaccggctg gtgagtcggg ccatcttcgc
     1741 ccagaccgat ggagtcgtgg gtgtaaacca tcacctggcg ctgtttcatc agcgcagcca
     1801 tacgtacggc gttacgtgcg tattccacga acatcaggaa ggtggaggtg tacggcagga
     1861 agccaccgtg cagggagata ccgttagcaa tcgcggtcat accgaactcg cgaacaccgt
     1921 agtggatgta gttacctgca gcatcttcgt tgattgcttt agaaccagac cacagggtca
     1981 ggttagacgg tgccaggtca gcagagccgc cgaggaattc aggcaacagc gggccgaacg
     2041 cttcgatagc attctgcgac gctttacggc tggcgatttt cgccggatta gcctgcagtt
     2101 tagcgataaa ctctttcgct ttggcgtcga agtcagacgg catttcgcct ttcatacggc
     2161 gggtaaattc agccgcttcc tgcggataag ctttcgcgta agccgcaaac ttctcattcc
     2221 atgcagattc tttcgcctgg cctgcttctt tcgcatccca ctgagcatag atttcagacg
     2281 ggatttcgaa cggtgcatat ttccagccca gctgttcgcg ggtcagggca atttcagcgt
     2341 cgcccagcgg cgcaccgtgg gagtcgtgag taccggcttt gttcggggaa ccgaaaccga
     2401 tgatggtttt gcacatcagc agggacggtt tgtcagtcac tgcgcgcgct tcttcaactg
     2461 cgcgtttgat ggatgccgcg tcatgaccgt cgatgtcgcg aataacgtgc cagccgtaag
     2521 cttcgaaacg cattgctgtg tcgtcggtga accagccttc aacgtgacca tcgatagaaa
     2581 taccgttgtc atcgtagaac gcaatcagtt tacccagctt cagcgtaccc gccagagagc
     2641 aaacttcgtg ggagatgcct tccatcatgc agccgtcgcc catgaaggcg taggtgtagt
     2701 ggtcgacaat gtcgtggccc ggacggttaa actgcgccgc cagcgttttt tctgcaatcg
     2761 ccataccgac tgcgttggca ataccctgac ccagcggacc ggtggtggtt tccacaccag
     2821 cggtataacc tacttccgga tggcccggag ttttagagtg cagctgacgg aagtttttca
     2881 gttcttccat cggcagatcg taaccggtga ggtgcagcag gctgtagatc agcatggagc
     2941 cgtggccgtt ggacagcacg aagcggtcac ggtcagccca ggacggattc tgcgggttgt
     3001 gtttcaggaa atcacgccac aggacttcgg caatgtcagc catacccata ggggcacccg
     3061 ggtgaccgga tttggctttc tgtactgcgt ccatgctcag cgcacgaata gcattggcaa
     3121 gctctttacg tgaggacatt ttgactccag atcggatgat gaagggcacg cccttaacga
     3181 cttgacgaca gcgcgttttg ggctacgccg gaaaatttgc caacaattta ccgcaagccg
     3241 cgcgtcatgt acatggaaca tccttttgcc gcttcagaaa tctctggatc atgctcgcat
     3301 gttgcgcaat ctgctcgccc gtccgctgcg cttttcctta tactgagact gagcgtcgat
     3361 tcacctgcaa acggcgcatt tttagaataa tcctgacctt gtgcggaaga gaaaacatga
     3421 aaattcgcgc cttattggta gcaatgagcg tggcaacggt actgaccggt tgccagaata
     3481 tggactccaa cggactgctc tcatcaggag cggaagcttt tcaggcttac agtttgagtg
     3541 atgcgcaggt gaaaaccctg agcgatcagg catgtcagga gatggacagc aaggcgacga
     3601 ttgcgccagc caatagcgaa tacgctaaac gtctgacaac tattgccaat gcgctaggca
     3661 acaatatcaa cggtcagccg gtgaattaca aagtgtatat ggcgaaggat gtgaacgcct
     3721 ttgcaatggc taacggctgt atccgcgtct atagcgggct gatggatatg atgacagata
     3781 acgaagtcga agcggtgatc ggtcacgaga tggggcacgt ggcgttaggc catgtgaaga
     3841 aagggatgca ggtggcgctt ggtacaaatg ccgtgcgagt agctgcggcc tctgcgggcg
     3901 ggattgtcgg cagtttatct caatcacaac ttggtgatct gggcgagaaa ttagtcaatt
     3961 cgcaattctc ccagcgccag gaagcagaag ccgatgatta ttcttacgat cttctgcacc
     4021 aacgcggcat cagcccggca ggtcttgcca ccagctttga aaaactggcg aagctggaag
     4081 aaggtcgtca aagctcaatg tttgacgacc atcctgcatc cgccgaacgt gcccagcata
     4141 ttcgcgatcg catcagcgcg gatgggatta agtaaagcct agtggtgtcg aacgtaggtc
     4201 agataaggcg ttcacgccgc atctgacatt aatggcacat ttgttacctt gtgcgcattg
     4261 ccggatgcga tgctggcgca tcttatccgg cctacgggtg ccgaacgtag gtcggataag
     4321 gcgttcacgc cgcatccgac attaatggca cgttttaccc gtgcgcatcg catctggtgc
     4381 ttactcgccc tttttcgccg cctgaatata cagcatctcc agcgccagcg tcgccgcagc
     4441 cagagcggtg atttccgact gatcgtatgc cggagccact tccactacgt ccatcccaac
     4501 gatgttgaga tctttcaggc cgcgtaccag tttaatggcg cgatcggagg tcaggccgcc
     4561 aatcactggc gtaccggttc ccggcgcgaa agcaggatcc aggcagtcga tatcaaaagt
     4621 caggtaaacc ggcatatcac ccacaatctg tttcacctgg gcgataacgt catccacgct
     4681 gcgatcgttc acctggcagg cgtccagcac ggtaaagccg ttgtctttat caaactcggt
     4741 acgaatacca atctgcacgg aatgattcgg gtcgatcaga ccttctttcg gcgcggtata
     4801 gaacatagtg ccgtggtcaa attcacaacc gttcgcatag gtatcggtgt gggcgtcaaa
     4861 gtgtaccagc gccattttgc cgaaatgctt cgcatgagca cgcagcagcg gcagcgtaac
     4921 aaagtggtca ccaccgaagg agagcatacg cttaccggca gccagcagct tctcggcgtg
     4981 cgcctgcagc ttttcgctca tctcacgggc atcgccaaag gcatatacca gatcgccgca
     5041 gtccacgacg ttcagacgct cacgcatgtc gaaattccac gggaagcggt tgtgttccca
     5101 ggccagattc gtcgaaacct gacggatcgc tgccggaccg tggcgaccac ccgcacgacc
     5161 agaagtggcc atatcgaacg gcacgccagt aatcacccag tctgcatcgc tgtcatacgg
     5221 ctggaagttc atcggcaggc gtaaaaaacc aaaggcattg gaaaccagtg agttatcgta
     5281 ttgatgacct aaggtgctca tggatggact cctattacaa agtcgatata aaaaaaccct
     5341 tccgcgtcgt taggcccgac gaggaagggt tggatttgcg aaacacaagt ttgtgctaat
     5401 tatcgcctta aatgggcgag gcatcaagcg aggttgctat ttccgacacg gtggcggcgc
     5461 atagccattg ttatctgccg gaaaggcttg tccagagcac ggcggcagcg cgttttgcgc
     5521 cgggccttgt aattcaaagg cggcaaagcc gattaccccg gtattacgca catcgccttg
     5581 cgccgaattg gctgcgtagg aatcagcaac gttggcgaac tgaaaagccg cttcagtatg
     5641 tttatctttg cggaatcctt taatcactaa cgaatctccg gcattcacaa tatagccgtt
     5701 gttattcagc gaaccttgct tgccgttcag cacatccagg ccatcagccg tagcaacgat
     5761 ctcgtagttg gtgttacgac tgtaattacg gacatataat tgataattca ttccga
//
LOCUS       DAZSNM010000220         5799 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZSNM010000220 DAZSNM010000000
VERSION     DAZSNM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.220"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            57..575
                     /gene="mddA"
                     /locus_tag="ACHW42_003610"
     CDS             57..575
                     /gene="mddA"
                     /locus_tag="ACHW42_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR8945261.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            572..1021
                     /locus_tag="ACHW42_003611"
     CDS             572..1021
                     /locus_tag="ACHW42_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR8945262.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1022..1255)
                     /gene="ydcY"
                     /locus_tag="ACHW42_003612"
     CDS             complement(1022..1255)
                     /gene="ydcY"
                     /locus_tag="ACHW42_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR8945263.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1341..1514)
                     /gene="ortT"
                     /locus_tag="ACHW42_003613"
     CDS             complement(1341..1514)
                     /gene="ortT"
                     /locus_tag="ACHW42_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR8945264.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1709..1804
                     /gene="yncL"
                     /locus_tag="ACHW42_003614"
     CDS             1709..1804
                     /gene="yncL"
                     /locus_tag="ACHW42_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR8945265.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2206..3015)
                     /locus_tag="ACHW42_003615"
     CDS             complement(2206..3015)
                     /locus_tag="ACHW42_003615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFR8945266.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3225..4649)
                     /gene="patD"
                     /locus_tag="ACHW42_003616"
     CDS             complement(3225..4649)
                     /gene="patD"
                     /locus_tag="ACHW42_003616"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR8945267.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4671..5465)
                     /locus_tag="ACHW42_003617"
     CDS             complement(4671..5465)
                     /locus_tag="ACHW42_003617"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8945268.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5455..>5799)
                     /locus_tag="ACHW42_003618"
     CDS             complement(5455..>5799)
                     /locus_tag="ACHW42_003618"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8945269.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1522 a         1472 c         1286 g         1519 t
ORIGIN      
        1 gggctgtttt cgatagtatt gataacatca tcatcattat tttattgagg ccagacatgt
       61 ccatccgttt tgcccgcaaa gccgactgtg ctgccattgc ggaaatttat aaccacgccg
      121 tgttgtatac ggcggctatc tggaatgacc aaacggtgga tgcagataac cgcattgcct
      181 ggtttgaagc gcggaccata gcaggttatc cggtgctggt aagtgaagaa gatggcgtag
      241 ttacgggata tgcctcgttt ggcgactggc gtagtttcga tggttttcgc cataccgtgg
      301 aacattcggt ttatgtccat cccgatcatc agggcaaagg tctggggcgt aaattgttaa
      361 gtcgattgat tgatgaagcg cgggattgcg ggaagcatgt catggtcgcc gggatcgaat
      421 cgcaaaatca ggcttcgctg catctgcacc agtcgctggg atttgtcgtc accgcacaaa
      481 tgccgcaggt aggcactaaa tttggtcatt ggctggatct gacatttatg cagttgcaac
      541 tcgacgagcg cactgaaccg gacgcgattg gatgaatcag tcgctcaccc ttgctttttt
      601 gattgccgcc ggaattggtc tggtagtcca gaacacgctg atggcgcgca tcacccagac
      661 ctcctctacc attctcatcg ccatgttgct gaactcactg gtggggattg tgctctttgt
      721 ttctatttta tggtttaaac aaggcatggc ggggtttggc gaactggtgt ccagcgtgcg
      781 ctggtggaca cttatacctg gcttgctggg ttcgtttttt gtcttcgcca gtatcagcgg
      841 atatcagaat gtgggagcgg cgacaactat tgcggtactg gtcgccagcc agctgattgg
      901 cggtctggtg ctggatatct tccgcagtca tggcgtacca ctacgggcgt tgttcggccc
      961 aatatgtggc gcaattttac ttgtggttgg cgcctggttg gtggccagac gctcatttta
     1021 atcagaggat ggtgccgcct ttggttaact gttcgtggcg cgcttccata tcttctttat
     1081 gcttgcgacc gtgatgagca atagcggtgc gcaaacgctg ctgctgggtg tagcgatctt
     1141 cccgacttaa ctccgcgtca tcgctcaggg cgattaataa ttcgttcata tgggcaatga
     1201 cgctctcttc aatcgcggca tccacgcgcg cgatgacttc atccagatgc gacatacttt
     1261 ctccttagcc acttgtgaaa tttatcgatt aatccaaatg atataagtct gcgtggtgcc
     1321 gtcaagccag tgagtaagat ttaaaacagt gaaaacaaca gcgccaccgg gaaactcatc
     1381 ggccatgtgg cccccaccag aaaggcgctt aagaaacgga tgcgtttctt atcgtgagaa
     1441 agaaaccagg tgataagaaa tgcgattgct gccataaccg cataaaaaac aagcatatgt
     1501 tgatagagaa acatgtacgg gcgtccagcc atatgaataa aacgcgcgca aatatgcgcc
     1561 attttttgac gtgcatcaag ttttttcacc agtttgagaa aaaatcatca aacagcaact
     1621 tgcccttatc catcttcaga atgagtatta ttgcggattt tcttaacccg tactatacac
     1681 ataggggcaa tgataaaagg tggcaaaaat gaatgtttcc agtagaactg tagtactgat
     1741 aaatgtcttt gctgctgttg gtttgtttac tcttatctct atgagatttg gctggtttat
     1801 ttgatgtagt gattttgcac gcagcacgta ctgtcagaga aatcaactct ctgacagtac
     1861 gcaagatctt ctcatactct ccaagtttgc aacattatcc ttaatattta cttctttttg
     1921 ctggcatcta cattgaaatg agtagaaaac atccccctac tgtcgtctat acaaacagct
     1981 tactgattca aacggaatct tgttttcaca tttttctatc acgaatactt tatacgcgcg
     2041 ggagtctata aatgctttat actttcaagg aataacgtaa atataaaaag atggcgtaaa
     2101 gtatatgcat ggcaggatgt ttgcaacctc cttgatgacg ctgccccatt gaaattgcaa
     2161 accccgccct ggttatcttg cccatttatc atgcaaaaca gataattata ggcctctgaa
     2221 tacctcagaa agattgaatt ttttatagtc cctctcttga atatttccat ttgatatcaa
     2281 tcgattcatc tcttcaacaa aataatttat atcatcacac ttattattat ccggaagatc
     2341 tgagtagtta taattccacc attttattct caacagttca tcaataacat ttgaatcaaa
     2401 cctaaatctg attatttttg caggaactcc tgcaactatg gcatatgggg gcacatcttt
     2461 ggtaacaacg gaattggcag caataacagc accatcacct attgcgatgc cacctttcaa
     2521 tacaacatca tttcctatcc agacatcatt tcctataatc ggattttcag gtttggtaat
     2581 gaatggtttc aatgtccagc tacgttcaaa ttcactcata gcaaacttat caaattcacc
     2641 gttataagtc atcatgtgag tagtaaaacg attcaaagga tgatttccac ccattatttt
     2701 tacatttgat gcaatactac aaaatcgacc tatacgtata ttccctgaaa aatggcaggt
     2761 cggaaatgaa aaagcgccga cactggagaa atttttcaca ggcattgttg aatattcttc
     2821 agcaataaca ttatcttcaa ttgttatctt gtcacctttt gagaaaagaa ataaattcct
     2881 ttctttatac gacttcttcc ccttatgatt taaaaagatt ttattatcca tacaaaactc
     2941 aaagtgtttt ttcgtccatc tgaaggtaag ggttttattt ttatcttccg tgaatttgta
     3001 caattgaact ttcattgtta atacttctac ttataaagta ttagtttaat aacgatactc
     3061 taaagcctta tgctaatcaa ctgtgacagg atgaaaaatc gccatcaaac atttagccta
     3121 atttttacgc cagttattaa tgtaatcatt ctaatactta accgccatta aactcagaac
     3181 ctaaaacgat agtggccgtc ctataacagc cactaacatc taacttaatg tttaaccatg
     3241 acgtggcgga cgacggtgta atcctccagc ccataaagtg acatatcctt gccgtaacca
     3301 gaaagtttct gcccaccgtg cggcatttca cttaccagca tgaaatgggt attgacccag
     3361 gtacaaccat attgcagccg tgcgctgacg cgatgcgccc tgcccacatc tttcgtccat
     3421 accgaagatg caagtccgta ctggctgtca ttcgcccagt tcaccacctg ttcttcgttg
     3481 tcgaagggcg taacactcac tactggacca aatacctctt tttgcacgat ggcatcgtcc
     3541 tgtaatgcgc cagccagcag cgtcggcgca tagtaatagc cattaccctt gcgcttttca
     3601 ccgccagtga tcactttgat gtgccctgtc gctttcgcct cttctactgc cttgctgacg
     3661 cgttcgagat gcgccagcga gcttaaaggt ccaagctccg tagactcgtc atccggcgca
     3721 ccagatttta acgttgccac cgcagcaccc agtttttcca ccagcgtatc gtaaatgcct
     3781 ttttgcgcgt agatccgaca agccgcagta caatcctgtc cagcattgta atagccaaat
     3841 gtacgtacac cttcgaccac tgcttcaata tccgcatcat caaaaacaat cactggcgct
     3901 ttgccaccaa gttccatatg agtacgctta atggacgacg cggtatggct gatgatgtgc
     3961 tcgccggtgg cgatagagcc cgtcagcgac accatccgca ctttgggatg accggtcagc
     4021 ggatcaccca ccgttttgcc tctgccaaac agtatgttaa tcacgcctgc cgggaagata
     4081 tctttcgcca gctctgccaa cttcaacgcg gtcagcgggg taatttctga tggtttaagc
     4141 actacgcagt tccctgccgc cagcgccgga gcaagtttcc acgcggccat catcagcgga
     4201 taattccacg gtgcgataga agccacgacc cccaacggat cgcgacggat catcgaagta
     4261 tgaccttcaa gatattcacc tgccgccaga ccattcagac agcgcgccgc acccgcgaaa
     4321 aagcgaaaaa catcgacaat cgccgggatt tcatcattga acgcactatg cagcggtttg
     4381 ccacaattac gggattccag ttcggcaaaa acctgaccat tttcttcgat aacatccgcc
     4441 agtttcagca ggcattccgc acgcgctttc ggtgtggttt gcccccattc agcaaatgct
     4501 gcatctgccg cacgcacagc agcatcgacc tgctctgcgg atacctcggc aatctccagt
     4561 aaaacgtccc ccgttgccgg attatagaca ggctgttttt ccccttcgcc gctaacccgt
     4621 tctccgttaa tcagtaactt atgttgcata gcttattcct gtatcagtgg ttattgacca
     4681 ttgtcgcctt cgcgggttag ccaccaggcc cccaggatcg gcaaggttgt taccaacata
     4741 accagcagtg ccaccacgtt ggttaccggt acatcacgcg gtcgcccaag ctgattgagc
     4801 aaccacaacg gtaacgttcg ttcatgacct gccgtaaagg tcgtaacgat gatttcatcg
     4861 aacgacaagg caaacgccag cattcctcct gccagtaacg ccgaactgag attcggcaac
     4921 actacgtagc ggaaggtttg ccagccattg gccccaagat ccattgacgc ctcaaccaga
     4981 ctccacgagg tgcggcgaaa acgggcgatg acattgttaa acaccaccac tacgcaaaaa
     5041 gtcgcatgac cgaccacgat ggtgaaaaat cccggctcca gattgatggt tttaaaggcg
     5101 gttaatagcg ccagaccagt gacaatgccc ggcagcgcaa tgggcagcag caataacagc
     5161 gaaatggcgt ttttgccgaa aaagtctcgt ctccacagcg cggcagctgc cagcgtccct
     5221 aacaccagcg caattaatgt cgccagcgcc gccactttaa gtgacagcgt cacggcatca
     5281 agaatatcac tacgctgtgc tgccacgcta aaccagcgca gcgtcaggcc ctgcggtgga
     5341 aaactaaacg ccgcatcttc agtgttaaac gcataggcgg cgattatcag gatgggaaaa
     5401 tgtaggaaaa caacgccgcc ccaggccgcc agtttgagga aaaacggtgc gcgttcagag
     5461 tgcatcgaac gctcccagac gtttcacgaa cgccaggtac agtgcgatga gaataatcgg
     5521 caccagggtg aatgccgccg ccatcggcat attgccaatc gccccctgct gggaataaac
     5581 catattgccg ataaaatatc ctggaggacc aaccagctgc gggacgataa aatcgcccag
     5641 tgtgagtgag aaggtaaaga tagagccagc ggcaataccc gggattgcca gcggcagcac
     5701 cacatagcga aaggtttgtc gtggacgtgc gccgagatca gccgacgcct gcaacaatga
     5761 cggcggcaaa cgctcaagcg ccgcctgaac gggcaggat
//
LOCUS       DAZSNM010000221         5786 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZSNM010000221 DAZSNM010000000
VERSION     DAZSNM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.221"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHW42_003619"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHW42_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR8945270.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="ACHW42_003620"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="ACHW42_003620"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR8945271.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="ACHW42_003621"
     CDS             complement(3736..4092)
                     /locus_tag="ACHW42_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR8945272.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="ACHW42_003622"
     CDS             complement(4096..4512)
                     /locus_tag="ACHW42_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR8945273.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHW42_003623"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHW42_003623"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR8945274.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1390 a         1498 c         1489 g         1409 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttg
//
LOCUS       DAZSNM010000222         5765 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZSNM010000222 DAZSNM010000000
VERSION     DAZSNM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.222"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1072
                     /gene="narU"
                     /locus_tag="ACHW42_003624"
     CDS             <1..1072
                     /gene="narU"
                     /locus_tag="ACHW42_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR8945275.1"
                     /translation="TAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMG
                     NISFFFPKAKQGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVM
                     SLANAAWIWVPLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFG
                     SFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFI
                     FMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRV
                     KMKGGSDEQAQREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFL
                     IFYIVCVLLTWLVYGRRKFSQK"
     gene            1154..4894
                     /gene="narZ"
                     /locus_tag="ACHW42_003625"
                     /pseudo
     CDS             1154..4894
                     /gene="narZ"
                     /locus_tag="ACHW42_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            4891..>5765
                     /gene="narH"
                     /locus_tag="ACHW42_003626"
     CDS             4891..>5765
                     /gene="narH"
                     /locus_tag="ACHW42_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR8945276.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1349 a         1551 c         1562 g         1303 t
ORIGIN      
        1 tactgcaatc ctgattattc cttgcgtctg gctcggaatt gccgtgcaaa atccgaatac
       61 tccttttggg atatttatcg ttatcgcttt gctatgcggt tttgcaggtg caaactttgc
      121 ttcgagcatg ggcaatatca gtttcttctt tccaaaagcc aaacaaggga gcgctcttgg
      181 gattaatggc ggattaggaa acttaggtgt aagtgtaatg cagctggttg caccgctggt
      241 catttttgta cctgtatttg cctttctcgg cgtcaatggc gtaccgcagg ccgacggttc
      301 ggtgatgtcg ctggcgaatg ccgcatggat ttgggtaccg ctactggcga ttgccacgat
      361 cgccgcctgg tcagggatga atgatatcgc cagttcacgc gcctcaattg ccgaccagct
      421 ccctgtctta caacgcctgc atctctggct gctgagcctg ctttaccttg ccaccttcgg
      481 ttcgtttatc ggtttttctg cgggttttgc catgctggca aaaacccagt tcccggatgt
      541 gaatattctg cgcctggcgt tctttggccc atttattggt gccatcgcgc ggtcggttgg
      601 tggtgctatt tccgataaat tcggcggcgt gcgggtgacg ttgatcaact ttatttttat
      661 ggcgattttc agtgccctgc tgttccttac cttaccgggc acaggctccg gtaatttcat
      721 cgccttttac gccgtattta tggggctgtt tctgaccgcg ggtctgggaa gtggttctac
      781 tttccagatg atcgccgtca tctttcgcca gataaccatt tatcgggtga agatgaaagg
      841 cggtagtgat gagcaagctc agagagaagc cgtcaccgaa acggcggcaa cgctgggctt
      901 tatctcagcc attggcgcag tgggcggctt ttttattccg caggcatttg gcatgtcgct
      961 caatatgacc ggctctccgg tcggcgcgat gaaagtgttt ttaatcttct acatcgtttg
     1021 tgtgctgctg acctggctgg tttatggtcg gcggaagttc agccaaaaat aagagatcga
     1081 tgcctgatgt acaaccatgt cgcatccgac aaggcatcag atcccaattc attcctggag
     1141 caggagtcat gtcatgagta aacttttgga tcgctttcgc tacttcaaac aaaagggcga
     1201 aacctttgcc gatggtcacg gacaggtgat gcatagcaac cgcgactggg aggacagcta
     1261 tcgccagcgt tggcagttcg acaaaatcgt gcgttccacc cacggcgtta actgtacagg
     1321 ctcctgtagc tggaaaatct acgttaaaaa tggtctggtg acctgggaaa tccaacagac
     1381 cgactacccg cgcactcgcc ctgacctgcc caatcatgaa cctcgcggct gcccgcgtgg
     1441 cgcaagttac tcctggtatc tttacagcgc taaccgcctg aaatacccgc tcattcgtaa
     1501 acgactgatt gaactgtggc gcgaagccct caaacaacac agcgatccgg tactggcgtg
     1561 ggcatcgatt atgaatgatc cgcaaaagtg cctgagctac aaacaagtgc gtgggcgcgg
     1621 cgggtttatc cgctccaact ggcaggaact aaaccagctg attgccgccg ctaacgtctg
     1681 gaccatcaaa acctacggcc cggatcgcgt tgccggtttc tcgccgatcc cggcgatgtc
     1741 gatggtttct tacgccgccg gaacgcgtta tctgtcgctg cttggcggca cctgttaaag
     1801 tttctacgac tggtattgcg acctgccgcc cgcctcgccg atgacctggg gcgagcaaac
     1861 cgacgtaccg gaatctgccg actggtataa ctccagctac atcatcgctt gggggtctaa
     1921 cgtaccgcag acacgtacgc cggacgccca cttctttacc gaagtacgct acaaaggcac
     1981 taaaaccatc gccattaccc ctgactactc tgaagtggcc aaattgtgcg accagtggct
     2041 ggcaccgaaa caaggcactg atagcgccct ggcgatggca atggggcatg tgattttaaa
     2101 agagtttcat ctcgataatc ccagcgacta ctttatcaac tactgccgcc gctacagcga
     2161 catgccgatg ctggtaatgc tggagcctcg cgacgatggt agctacgttc ccgggcgcat
     2221 gatccgcgca tctgacctgg tggatggact gggcgaaagc aacaatccgc agtggaaaac
     2281 cgtagcagtt aataccgcag gtgaattggt agtgccgaac ggttcgattg gtttccgctg
     2341 gggagaaaaa ggcaaatgga atctggaatc cattgccgcc ggtacggaaa ccgaattgtc
     2401 gttaaccctg ctcggtcaac atgacgctgt tgcaggcgtg gccttcccct actttggcgg
     2461 cattgaaaat ccgcattttc gcagcgtaaa acacaatccg gtgctggtgc gccaattgcc
     2521 cgttaaaaac ctgacgttag tcgatggcaa cacctgtccg gtggtcagcg tttatgattt
     2581 ggtactggcg aattacggcc tcgatcgcgg gctggaagat gaaaacagcg cgaaagatta
     2641 cgctgaaatc aaaccgtaca ccccagcctg gggtgagcaa attaccggcg taccgcgcca
     2701 gtatattgaa accatcgccc gcgaatttgc cgataccgcc cataaaacgc atgggcgctc
     2761 aatgattatc ctcggcgcag gtgttaacca ctggtatcac atggacatga actaccgcgg
     2821 gatgatcaat atgctgatct tctgcggttg tgtcgggcaa agcggtggcg gctgggcaca
     2881 ctatgtcggc caggaaaaac tgcgcccaca aaccggctgg ttgccactgg cctttgcgct
     2941 cgactggaac cgaccaccgc gccagatgaa cagcacctcg tttttctaca atcattccag
     3001 ccaatggcgc tatgaaaaag tctctgctca ggagttactt tcaccgctcg ccgatgccag
     3061 taagtacagc ggtcatctga ttgatttcaa cgttcgcgcc gaacgtatgg gctggctacc
     3121 ttctgcgccg cagctggggc gtaacccgct cgggattaaa gctgaagccg acaaggccgg
     3181 attatccccc acagaattta ccgcccaggc gctgaaatcg ggcgatttac gtatggcctg
     3241 cgaacaacca gatagcggca gcaatcatcc gcgtaatttg tttgtctggc gctctaacct
     3301 gcttgactcc tccggcaaag gccacgagta tatacagaag tatctgctgg ggaccgaaag
     3361 cgggattcag ggcgaggaac tcggtgccag cgacgggatc aaaccggaag aagtcgagtg
     3421 gcaaactgcg gcgattgaag gcaagctcga cctgctggtg acgctcgact tccgcatgtc
     3481 cagtacctgc ctgttctccg atatcgttct gcccaccgcc acctggtatg aaaaagacga
     3541 tatgaacacc tcggatatgc atccatttat tcatccgctt tctgcggcgg tcgatccggc
     3601 ctgggaatca cgcagcgact gggaaatcta caaaggtatt gccaaagcat tttcgcaagt
     3661 gtgcgtgggc catcttggca aagaaaccga cgtggtatta caaccactgc tgcatgactc
     3721 tccggcagag ctctcacagc cgtgtgaagt gctcgactgg cgcaaaggcg aatgcgatct
     3781 gatcccgggt aaaaccgcgc cgaatattgt ggcggtggag cgcgactacc ctgctacgta
     3841 tgaacgcttt acctcgctcg ggccattgat ggacaaactt ggcaatggcg gtaaagggat
     3901 ttcgtggaat acccaggatg aaatcgattt cctcggcaaa ctcaattaca ccaagcgtga
     3961 tggcccggca caggggcgtc cgctgattga caccgccatt gacgcttcag aagtgattct
     4021 ggcactggca ccggaaacca acggtcatgt tgcagtcaaa gcgtggcagg cgctgggcga
     4081 gatcaccggg cgcgaacata cccatctggc gctgcacaaa gaggacgaga agattcgctt
     4141 tcgcgatatt caggcgcagc cgcgtaaaat tatctccagc cccacatggt ccggtctgga
     4201 aagcgatcac gtatcctata acgcgggata caccaacgtt catgagttaa ttccgtggcg
     4261 cacgctgtcg ggacgccagc agctctatca ggatcatccg tggatgcgtg cttttggtga
     4321 aagcctggtg gcttatcgcc cgcctatcga cacccgtagc gtcagtgaga tgcgccagat
     4381 accgccaaac ggcttccagg aaaaagcact taacttcctg acgccgcacc agaaatgggg
     4441 cattcactca acctacagtg aaaacctgct aatgctgacg ctctctcgcg gtggaccgat
     4501 tgtctggatc agcgaaacag atgcccgaga actgaccatt gtcgataacg actgggtgga
     4561 agtatttaac gccaacggcg cgctgactgc ccgcgcggtg gtcagccaac gtgtaccgcc
     4621 gggcatgacc atgatgtatc acgcccagga acgcattatg aatattcctg gttcggaagt
     4681 aactggcatg cgcggcggca ttcataactc ggtcacccgc gtttgcccga aaccgacgca
     4741 tatgattggc ggttacgcgc agctggcctg gggctttaac tactacggca ccgtcggttc
     4801 gaaccgcgac gaattcatca tgatccgcaa gatgaagaac gttaactggc tggatgatga
     4861 aggtcgcgat caggtacagg aggcgaaaaa atgaaaatac gttcgcaagt cggcatggtg
     4921 cttaacctcg ataaatgtat cggctgccat acctgttcgg tgacctgtaa caacgtctgg
     4981 accggacgcg aaggcatgga gtacgcatgg tttaacaacg tcgaaaccaa accgggcatt
     5041 ggttatccga aaaactggga agatcaggaa gagtggcaag gcggctgggt ccgcgatgtg
     5101 aatggcaaga tccgtccgcg tctgggcagc aagatgggcg taataaccaa aatcttcgcc
     5161 aacccggtgg tgccgcagca gattgatgat tactacgaac ctttcacctt cgactacgaa
     5221 catttgcata gcacaccgga aggcaaacat atccctactg ctcgcccgcg ttcactgatt
     5281 gacggcaaac ggatggacaa agtgatctgg ggcccaaact gggaagaact gctgggcggc
     5341 gagtttgaaa aacgtgcccg cgaccgcaac ttcgaggcca tgcaaaagga gatgtacggg
     5401 cagtttgaaa acaccttcat gatgtacctg ccgcgcctgt gcgaacactg cctcaatccc
     5461 agctgcgtgg cgacctgccc aagcggcgct atctacaaac gcgaagaaga cggcattgtg
     5521 ctgattgatc aggataaatg ccgtggctgg cgtttgtgca ttagcggttg tccgtacaaa
     5581 aaaatctact tcaactggaa aagcggcaag tcagaaaaat gtatcttctg ttatccgcga
     5641 attgagtcag gccaacccac cgtgtgctca gaaacctgcg tgggtcgcat ccggtatctg
     5701 ggcgtgctgc tttacgacgc cgaccgcatc gaggaagcgt cgagcaccga gcgcgaagtt
     5761 gacct
//
LOCUS       DAZSNM010000223         5738 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZSNM010000223 DAZSNM010000000
VERSION     DAZSNM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.223"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..241
                     /locus_tag="ACHW42_003627"
     CDS             <1..241
                     /locus_tag="ACHW42_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945277.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="ACHW42_003628"
     CDS             complement(238..1179)
                     /locus_tag="ACHW42_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HFR8945278.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="ACHW42_003629"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="ACHW42_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="ACHW42_003630"
     CDS             complement(1684..2904)
                     /locus_tag="ACHW42_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR8945279.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="ACHW42_003631"
     CDS             complement(2908..3651)
                     /locus_tag="ACHW42_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR8945280.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="ACHW42_003632"
     CDS             complement(3542..5008)
                     /locus_tag="ACHW42_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HFR8945281.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTAMGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5738)
                     /locus_tag="ACHW42_003633"
                     /pseudo
     CDS             complement(5008..5738)
                     /locus_tag="ACHW42_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
BASE COUNT         1226 a         1618 c         1434 g         1460 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc catcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DAZSNM010000224         5713 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZSNM010000224 DAZSNM010000000
VERSION     DAZSNM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.224"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            219..1151
                     /gene="rfaD"
                     /locus_tag="ACHW42_003634"
     CDS             219..1151
                     /gene="rfaD"
                     /locus_tag="ACHW42_003634"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR8945282.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1161..2207
                     /gene="rfaF"
                     /locus_tag="ACHW42_003635"
     CDS             1161..2207
                     /gene="rfaF"
                     /locus_tag="ACHW42_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR8945283.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2211..3203
                     /gene="rfaC"
                     /locus_tag="ACHW42_003636"
     CDS             2211..3203
                     /gene="rfaC"
                     /locus_tag="ACHW42_003636"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR8945284.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3200..4408
                     /gene="rfaL"
                     /locus_tag="ACHW42_003637"
     CDS             3200..4408
                     /gene="rfaL"
                     /locus_tag="ACHW42_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFR8945285.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(4444..5586)
                     /locus_tag="ACHW42_003638"
     CDS             complement(4444..5586)
                     /locus_tag="ACHW42_003638"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFR8945286.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(5595..>5713)
                     /locus_tag="ACHW42_003639"
     CDS             complement(5595..>5713)
                     /locus_tag="ACHW42_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR8945287.1"
                     /translation="YKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNVMKG"
BASE COUNT         1578 a         1243 c         1279 g         1613 t
ORIGIN      
        1 ccgttattta tcatacatcg gaatattgat actaaagcac tattcacatg caaaaccaac
       61 atccgccatg aaggactagc taaaacccaa actagtttgt tgcaattagc atccttgcac
      121 ctctatgtaa agggctgaag ggattcggat gtgatggtat gattacagac attcgtgtct
      181 gagattgtct ctgactccat aattcgaagg ttacagttat gatcatcgtt accggcggcg
      241 cgggctttat cggcagcaac atcgttaagg ccctgaatga taaaggcatc accgatattc
      301 tggtggtgga caacctgaaa gacggcacca agtttgtgaa cctggtggat ctggatatcg
      361 cggactatat ggataaggaa gacttcctga tccagattat ggctggcgaa gagttcggtg
      421 atgtcgaagc gattttccac gaaggcgcat gctcttccac caccgagtgg gatggcaagt
      481 atatgatgga taacaactat caatactcca aagagctgct gcactactgt ctggagcgcg
      541 aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg cggacgcacc tccgacttta
      601 ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg ttactcaaaa ttcctgtttg
      661 atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca gattgttggc ttccgctatt
      721 tcaacgttta tggaccgcgt gaaggccata aaggcagcat ggcgagcgtc gctttccatc
      781 tcaacaccca gcttaataac ggtgaatcac cgaagctgtt cgaaggtagc gagaacttca
      841 aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa cctgtggttc ctggaaaatg
      901 gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga atccttccag gcagtagcag
      961 atgctacgct ggcttatcac aagaaaggcc agatcgaata cattccgttc ccggataagc
     1021 tgaaaggccg ctaccaggcg ttcactcagg cagatctgac aaatctgcgc gcggcgggtt
     1081 acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga atacatggcc tggctgaatc
     1141 gcgacgcata agagctctgc atgaaaatac tggtgatcgg cccgtcttgg gttggcgaca
     1201 tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg ctatccccag gcgataatcg
     1261 atgtgatggc accggcatgg tgccgtccat tattatcgcg gatgccggaa gttaacgaag
     1321 ctatccctat gcctctcggt cacggagcgc tggaaatcgg cgaacgccgc aaactgggtc
     1381 atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt accaaactcc ttcaaatctg
     1441 cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg ctggcgcggc gagatgcgct
     1501 acggtttact caacgatgta cgcgtgctcg ataaagaagc ctggccgcta atggtggaac
     1561 gctatgtcgc gctggcctat gacaaaggca ttatgcgtac cgcacaagat ctgccgcagc
     1621 cattgttatg gccgcagttg caggtgagcg aaggtgaaaa atcatatacc tgtaatcaat
     1681 tttcgctttc atcagaacgt ccgatgattg gcttttgccc gggtgcggag tttggtccgg
     1741 caaaacgctg gccacactac cactatgcgg aactggcaaa gcagctgatt gatgaaggtt
     1801 atcaggtggt tctgtttggc tctgcgaaag atcatgaagc gggcaatgag attcttgccg
     1861 ctttgaatac cgagcagcag gcatggtgtc ggaacctggc gggggaaaca cagcttgatc
     1921 aagcggttat cttgattgca gcctgtaaag ccattgtcac taacgattct ggcctgatgc
     1981 acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta tggtccaagt agcccggact
     2041 tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct gattaccggc tatcacaaag
     2101 tgcgtaaagg tgacgctgcg gagggttatc accagagctt gatcgacatt actccccagc
     2161 gcgtactgga agaactcaac gcgctattgt tacaagagga agcctgacgg atgcgggttt
     2221 tgatcgttaa aacatcgtcg atgggcgatg ttctccatac gttgcccgca ctcactgatg
     2281 cccagcaggc aatcccaggg attaagtttg actgggtggt ggaagaaggg ttcgcacaga
     2341 ttccttcctg gcacgctgcc gttgagcgag ttattcctgt ggcaatacgt cgctggcgta
     2401 aagcctggtt ctcggccccc ataaaagctg aacgcaaagc gtttcgtgaa gcgctacaag
     2461 cagagaacta tgacgcagtt atcgacgctc aggggctggt aaaaagcgcg gcactggtga
     2521 cacgtctggc gcatggcgta aagcatggat tggactggca aaccgctcgc gaacctttag
     2581 ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca gcagcacgcc gtagaacgca
     2641 cccgcgaact gtttgccaaa agtttgggct atagcaaacc gcaaacccag ggcgattatg
     2701 ctatcgcaca gcattttctg acgaacctgc ctacagatgc tggcgaatat gccgtatttc
     2761 ttcatgcgac gacccgtgat gataaacact ggccggaaga acactggcga gaattgattg
     2821 gtttactggc tgattcagga atacggatta aacttccgtg gggcgcgccg catgaggaag
     2881 aacgggcgaa acgactggcg gaaggatttg cttatgttga agtattgccg aagatgagtc
     2941 tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt agtgtcggtg gatacggggt
     3001 taagccattt aacggcggca ctggatagac ccaatatcac ggtttatgga ccaaccgatc
     3061 cgggattaat tggtgggtat gggaagaatc aagtagagtg tcgttctaca agtatgtctc
     3121 ttgcagattt gccagctcaa accgtttttc aaaatttaaa tcttgaaata ataaccaata
     3181 agttgacatc ggagataaga tgacctcaac attatttttc tctctcgaga aaaaaaactg
     3241 gatagcgtac tggaacagag ctctcgtatt cttattcatt accacctatt ttttgggtgg
     3301 gattacaagg tataaacatc ttattgttat tcttatgaca ataacgacaa tcgtctatct
     3361 ctgcaaacgg ccaaaacact atctctcact atttaaaaca tttctttttg gtagtgttgc
     3421 catattaact attgctgcat tgctgtcact tcttcaatcc cctgatgcag gtgctagcat
     3481 gaaggaagtt ttcaaagcta ttattgagaa tactttacta tgcacaatag caataccggt
     3541 catattgaga gacgagaaaa gagaagatgt cgaaaaaatc gttttcttct catttattag
     3601 tgcgttgggc ttacgctgtt tttctgaatt gattacctat tataaggact atcaacaagg
     3661 gataatgcca ttcgcagatt atagacaccg tagcatttct gactcgatgg tctttttatt
     3721 ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa taccgcattt cttttgtggt
     3781 tctaagcgtt atttttattt ttctgatatt aggaacttta tccagagggg cctggctttc
     3841 cgtgttagtc attggattaa tatggattct gatgtttaaa caatggaagt tactattagt
     3901 aggagtaatg gtttccatca ttgcattgtc ggttattttc acacataagg agatgaccgc
     3961 aaagctaacg tataaacttc aacaaactaa tagttcttat cgctatgcaa atggtactca
     4021 aggcagcgca ctcgatctaa tattagaaaa tcctgttatt ggttatggtt acggtaacgt
     4081 tgcatataaa gatgtctata ataaacgtgt cattgattat ccagaatgga cctttagaca
     4141 atcaataggg ccacataatt ttgcgctatt catctggttt ggcactggtt tattagggct
     4201 ggtaagtctt atgatgctat actgtgcaat attgaaagag tgtataaaaa atggcgtcaa
     4261 gaataaatat cgctcaccat ataatgcata ttatataatc ttactatctt ttataggtta
     4321 ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat ttattaggcg tttacgccgg
     4381 cttattatta gcgatgaaaa acaagtaaaa aataaaaaag gctgcataat gcggcctttt
     4441 tatttattca aaccaattat gaataacctc ttcgaatctt tgagttacgc catcccaact
     4501 gtagtgatca aacacaaagt cctgaccttg ctttgcaact gctgttaatt caggattcgc
     4561 taacgttttt aaaatatcgc tgcttatgga gtctgcagtc ataggttctt ttaaatggaa
     4621 tcccgtagta ttttctttga caaattccgt catcccccca cgcgtactca ctaaaacagg
     4681 cttaccagca cccatagctt caattgccac catgcaaaat ggttcctgga actgggaagg
     4741 aatgacgact aaatctgcca gtgggtaata acaatacatc ttttcaggcg gtatgcttcc
     4801 taacataatg caacgatcct tcaaacgttt tgcaatttcc ctgacattgc gttgatatgc
     4861 acctttgtca cttttactca tttctgtata atcaccaatc acaactaatt ttaaattttt
     4921 gtgagcagcg gccaattttt cgaatgattg catcaacagc aatatgcctt tgtcagggac
     4981 gatacgccca gcataaaaaa taattttctc ttcaggagag atattaagtt cgcttctagt
     5041 gatcggctga aaattagatt gataggtttc taaatcaatt ccatttggta caatctcaat
     5101 atctgcattt gctaaataag attggtaata ctttttcaag tataaactag ggactatcat
     5161 tttaacattt tgctcaagca attttggttc aaatgcatta tgcatatgta taaccactct
     5221 tgcctgagga gctagcttac gaatttgagt gtatagcttc atgctattat gaactacaat
     5281 aacactttct ttcgttatag gaaaatcatg tgcaatattc aatattctct gtgaatatgg
     5341 aagaggatca agtcttgtcc atttttgaaa taaccgctta tatatgcgac tgaatccaac
     5401 cctatgaata ctacaattat cattaacaaa ggtgtaattt gagtatcctg gatttttgat
     5461 acaaacaata cgatttggaa aattggtacg ctgagcaacc tgatacatcc acgtttcaac
     5521 agcagccgct ccgcgaggag gaattgaaaa aataggagta accgtaaata ttattttatc
     5581 aaccatctcg tatattaacc tttcataaca ttatatttaa ttgctgtaaa aacaccatcg
     5641 agaaattttt tctggtaaag caagtgttta tatttttctt tgtgctcatg ttttttaaca
     5701 gcttttttat aag
//
LOCUS       DAZSNM010000225         5710 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZSNM010000225 DAZSNM010000000
VERSION     DAZSNM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.225"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..248)
                     /locus_tag="ACHW42_003640"
     CDS             complement(<1..248)
                     /locus_tag="ACHW42_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8945288.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
     gene            complement(335..696)
                     /locus_tag="ACHW42_003641"
                     /pseudo
     CDS             complement(335..696)
                     /locus_tag="ACHW42_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1035..1589)
                     /locus_tag="ACHW42_003642"
                     /pseudo
     CDS             complement(1035..1589)
                     /locus_tag="ACHW42_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1732..1854
                     /locus_tag="ACHW42_003643"
                     /pseudo
     CDS             1732..1854
                     /locus_tag="ACHW42_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="ACHW42_003644"
     CDS             complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="ACHW42_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFR8945289.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2964..3278)
                     /locus_tag="ACHW42_003645"
     CDS             complement(2964..3278)
                     /locus_tag="ACHW42_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945290.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3356..3466)
                     /locus_tag="ACHW42_003646"
                     /pseudo
     CDS             complement(3356..3466)
                     /locus_tag="ACHW42_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="ACHW42_003647"
     CDS             complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="ACHW42_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HFR8945291.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4839..4976)
                     /locus_tag="ACHW42_003648"
                     /pseudo
     CDS             complement(4839..4976)
                     /locus_tag="ACHW42_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5058..5356)
                     /locus_tag="ACHW42_003649"
                     /pseudo
     CDS             complement(5058..5356)
                     /locus_tag="ACHW42_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1543 a         1238 c         1162 g         1767 t
ORIGIN      
        1 cgagcgatta ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg ttgacgaata
       61 taatcccggt attcatccag gagtgaagca acagcaggtc gcggcgtata ttttggcggc
      121 tcagattttg cctgcaaata acgtttaacg gtattgcggg agagccccag ttctctggca
      181 atcgcccggc tactcattcc ctgcttgtgc aggattttaa tttccataac tgtctcaaaa
      241 gtgaccataa gctctcctga atcaggagag cagattaccc cctggatctg atttcaggcg
      301 ttgggtgtgg atcactattg caccgttcgt gacagtgttg ttgcgccttg ctgccaacga
      361 agctccactg cccatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag
      421 atgttatccg ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt
      481 cagcgtacgg ccagtatcgc gaactccggc accgttgaaa gccatttcag tgatttggtt
      541 tttaactccc ggtttacggg cctcgtaaca gtaagtgagc tgaaacaccc ggtgacagtc
      601 acggcaacgg aatcgatcat gtccttttgg gttctgcccg tgacggtaaa cctgaacgga
      661 ctggcaacgg gggcaatgaa agttaactgt gaccatgaaa gaacctaaaa gcgggtatta
      721 taacccctaa ttcaactaat tggaggcatt gtcattttta atctatggat acacttaaaa
      781 atcctctcac cttacgttaa aaaccttctc atgtttagag ttttttatta tgtttatcat
      841 aatgatatga cgatctgagt ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac
      901 agtgcctgtg gataactttg cgtaaatccg gaggacagat cactctgaag caagacaaca
      961 gcatctgaaa acaatatgct cacgtttcaa ccacagggcg taagtgatcc tacccacgta
     1021 atatggacac aggcctaagc gaggttctgg ttttcaaatt gttccggact gaggccgcca
     1081 caccaactgt gccgtcgcca gcgattgtaa tcacattcga tataattaaa cactgttgcc
     1141 ctcattattt cccggctgat aaagtgttct ccgtggagac attccacttt cagcgaatga
     1201 aagaagcttt ccacgcaggc attatcgtag cagcagccct ttgcgctcat actgccacgc
     1261 agattatgcc acttcagcag cgcctgataa tctgctgaac agtactgact tcctcggtcc
     1321 gagtgaacga taacgttccg gggtctctta cgccgccaca gcgccatctg cagggcatcg
     1381 caggccagtt gcgccgtcag gcgtggtgac atggaccagc caataacggc acgtgaccac
     1441 aggtcaatga ccacagccag atacagccag ccttcatctg tacgtaagta cgtgatgtct
     1501 cccgcccact tctggttcgg accactggcg taaaaatcct gctccagcag attttctgac
     1561 acaggcaggc catgtgcgcg gtaactgacc gtattgctgg gtaagatcaa tttgctcact
     1621 ggtatttact acgtgcatcg aatgagtgat caagagccgt atgacgcgag agtgtcacgt
     1681 acggttccgt aagcagccgg agagtgaaac tctctccggc tgactcgact cgcaggttcc
     1741 gacagcggtg acgaacatgc agcggtgttg ttctcgctga tcggcacatg ccgtctgaac
     1801 aatgtggagc cagaaaaatg gctgcgttac gttattgaac atatccagga ctgaccggca
     1861 aaccgggtac gcgatctgtt gccttggaaa gttgatctga cttctcagta aatatcaata
     1921 tgattctgac gagccgctta cctttgagca gagaaagggg agagggatcc cacatccatg
     1981 agctaggaaa atacaaaaac tggctataca acaattcaat atattaggac ttacttgcga
     2041 ggagaaatat agcaccgctc aggcaattcc tgggtattta cttttcttag tttctttact
     2101 cgggatgttc tgtcatcaga aaggcgattc taaatttgta atatagtcac tcatattccc
     2161 acgtaatttc tttagatcaa tatattgcac atttttctca aaaaactgat cggaaataag
     2221 atttgcaaca ttgcttgttg tatcattttt aacgcttcta ttcatccctt cacaaagttc
     2281 gtccattttc tttttgaaat aactgcttcc tgatacaaga tgatttatgg aactttgtgc
     2341 agcattgatg tctagagaaa tgcgataatc tttctcaacc tgtttaaata tctgtttacg
     2401 gagattatcg ccgatttttc ggctgtgttc tgcacaaaac ttatcaatac attggtttac
     2461 cacccgggat acgtagactt ttttttcatc atttttccaa tctgtaatat tttcaccgat
     2521 ttgttctgaa acagagctca aaatactagt atttattttg gttgatgata tttttactat
     2581 ccttttcccc attgtacagc ggataatctc atccaccttt cctgaaaaat atcttttgtg
     2641 agataaactg attccaaaat tattaaaaga tgttccaagc attataatca ccttgtcatg
     2701 taaaatagta acattgtcat tattttagct gattcaaaca attctgtaaa tgctttgagt
     2761 tctttactcc cttggctcaa tgatggaccg tctggaatcc cagagactaa actctctgag
     2821 aaagagagta tgaaaaaact tggggcagtc cctaaatgaa gggaccaaca gcggtaaaat
     2881 aaccggcatc attcataatg atgccctgtt agttgtagtt atgaagattt acgtcattaa
     2941 aattgctgtt cgtagtgtca gtcctatggt ctggcatcag ctgaggatcg tagccgatac
     3001 atcactggct tcacgacatt cgtgttgagg ctattcatga aaactccacg ctgaaaacgc
     3061 cgttttgtat aaacggtcac ggtatgcccg gagccactgt tgctgataaa ttcgataaaa
     3121 ccttggcgtt tcttgaagct atcgttaatg cggataatga aacaacgatt ggtgagattc
     3181 gctcttttgc cgacgccctg gacgctgtcc ggttcaatcg caacaaaatc aaccggcagc
     3241 tgagcaaacc caaccttgcc tctctggcgc tggagcatga agtgatttgg ctgggacgta
     3301 gccgctgaca gcttctataa aagtatgatc ctacccacgt aatatgaaca caggcttaag
     3361 tgaggttctg gttttcaaat tgttcaggac tgagacctcc acaccaactg tgccgtcgtc
     3421 agcgattgta atcgcacttg atataattaa acactgtata tttcatttgt ggagtctcca
     3481 gtctgagagg gctttatgca ttccgtattg ggcactatga tgccagaaat ctgcaaggct
     3541 ccacaccttc ctgtatttcc cttcctctat cacagtgcac tttacttaat agggtgggat
     3601 gtgttcatta catttattgt gttttttatg attgtttttg cgtccagtgt cgttcgtcat
     3661 gggaaactgg ttctatgggt agtatcgctc agtctagtta actgtttgtt tctttttaga
     3721 aattaagcag atcacacaat tcggggagag atccttctaa gtgcaattct ttgcagctat
     3781 ttagcacact taaaacaact cttgtatttt gtcagaaata atattaaaca tgtcttgttc
     3841 attattgtta agtctgtctg ctatttctgt aagttttatc ccactatgat ggcatatatt
     3901 tatatatgcc atcatagcct cgatattccc tttccctgcg gccagaaata atcctggtaa
     3961 cccctcatta tctcttacat ccaatagttt aatcgtttct tttggtgata aatcaaaagt
     4021 tttaataaaa tctccataag cttgaatcgc agcggaatga ccttcttgaa gagctatgaa
     4081 caaagcagat tccccagcat tatttcgtgc tgcaagaact tctagttttt cctctttggg
     4141 tatttcggct gccaacagcg attcaccgta tttacgcgct gcatcggcat ttccatgcca
     4201 aatcaatgaa aacaaattat ggcggttata ctccttatta ttctttgtcg gaatggatat
     4261 gttctgtatt ttggccaatt ttctgaaaga aacaccacta tatccccccc ctgatttttc
     4321 ttcattgatg gcgtgggata tagcggtatt tgtcacgtta atgatattat ttttcattcc
     4381 tttattttca ttagcagtat tgctgcctat tattaagtgc atatttttgt tgtttgctgg
     4441 atgtaatcca tagttattta ttgacatttt tgttcatcct ttttgctaaa aaaaaacaat
     4501 aacctatttt tgtaatgtaa tagatattag tctattgcta atctaaaaaa accattgcca
     4561 cattgaatgg ctatattcac caataaattc tttcgatttc gtatattgac atatcgtaga
     4621 tatatcttat ccctaagatt caataatcga gtccgtttac cattctgtgc aagcgccttc
     4681 actcagaagt gaccgtcaag gcgatcacca aacttgataa taaagcagct cattgccata
     4741 cgccagtcct taaggggcgt tgtccatttc tgtgatgctg cctagattta actgccagct
     4801 ccggcttatc tacatctaaa tatgtgagcc taataccgct acactttgtc cagactatgt
     4861 ttttctccgg gaagtgggct aaccgtctcc ataaagtgac gaactttttt caacagtggc
     4921 catattgaac ggcactgatg attacgggtt attgtctcat gaagagccgg ccacatcact
     4981 gacagcagtg acttcaggcc ttcagcacca gacagcctga accagttaat ccatcgcccg
     5041 acaaatttag taatctaggg ctggtttttc ttcagctgtt gcggttcgaa agtgccatta
     5101 cgatcacgca gagtacggat ttccagaggt tcatctccag tagtgataat cttcgttaag
     5161 caaccattgc gggaattgct gccagatcta agctgatttt tctcgcaccc aagatgatga
     5221 gtcatattag cattgagtac ggtttcattt tcagcatcca atcaaactga ctgagatctt
     5281 caggattttt gagacttttg gctagttcat tagctaaagc ccgcaactta ttagatgtca
     5341 actgtttttc attcataaat tcaccttcat ttgatatcaa attaaatagt tcaacatcag
     5401 agaattatac aaacttatgt acaggctcct atgatcttcg aatcctgaag attatagtga
     5461 aaataagttt tcacgttgaa atcccacata gttagtatat gataaataat catggatgac
     5521 ctgattaacc tcttggggta gtgcttttat agaagaataa atattctact aatttaatac
     5581 atcacctaaa atagtattgt tttttatggt tggttgaagg tcgtggtgat gatgttagtc
     5641 cttttgagaa tattccagtg ccagatctta cttataataa aggggcttgg cagtttggat
     5701 aaactattgt
//
LOCUS       DAZSNM010000226         5702 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZSNM010000226 DAZSNM010000000
VERSION     DAZSNM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.226"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..170)
                     /locus_tag="ACHW42_003650"
     CDS             complement(<1..170)
                     /locus_tag="ACHW42_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945292.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="ACHW42_003651"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="ACHW42_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR8945293.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="ACHW42_003652"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="ACHW42_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHW42_003653"
     CDS             complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHW42_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR8945294.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHW42_003654"
                     /pseudo
     CDS             4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHW42_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
     gene            5484..>5702
                     /locus_tag="ACHW42_003655"
     CDS             5484..>5702
                     /locus_tag="ACHW42_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFR8945295.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAZSNM010000227         5622 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZSNM010000227 DAZSNM010000000
VERSION     DAZSNM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.227"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="ACHW42_003656"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="ACHW42_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR8945296.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1102..1185
                     /gene="yqiM"
                     /locus_tag="ACHW42_003657"
     CDS             1102..1185
                     /gene="yqiM"
                     /locus_tag="ACHW42_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR8945297.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1207..1869
                     /gene="yqjA"
                     /locus_tag="ACHW42_003658"
     CDS             1207..1869
                     /gene="yqjA"
                     /locus_tag="ACHW42_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR8945298.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="ACHW42_003659"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="ACHW42_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR8945299.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /gene="yqjC"
                     /locus_tag="ACHW42_003660"
                     /pseudo
     CDS             2403..2757
                     /gene="yqjC"
                     /locus_tag="ACHW42_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
     gene            2795..3100
                     /locus_tag="ACHW42_003661"
     CDS             2795..3100
                     /locus_tag="ACHW42_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR8945300.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="ACHW42_003662"
     CDS             3103..3507
                     /locus_tag="ACHW42_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR8945301.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="ACHW42_003663"
     CDS             3497..3796
                     /locus_tag="ACHW42_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR8945302.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="ACHW42_003664"
     CDS             3982..4278
                     /locus_tag="ACHW42_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR8945303.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="ACHW42_003665"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="ACHW42_003665"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFR8945304.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
BASE COUNT         1431 a         1376 c         1410 g         1405 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtaga gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gc
//
LOCUS       DAZSNM010000228         5600 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZSNM010000228 DAZSNM010000000
VERSION     DAZSNM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.228"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..411
                     /locus_tag="ACHW42_003666"
     CDS             <1..411
                     /locus_tag="ACHW42_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8945305.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="ACHW42_003667"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="ACHW42_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACHW42_003668"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACHW42_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HFR8945306.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1332 g         1488 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc
//
LOCUS       DAZSNM010000229         5590 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZSNM010000229 DAZSNM010000000
VERSION     DAZSNM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.229"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..279)
                     /locus_tag="ACHW42_003669"
     CDS             complement(1..279)
                     /locus_tag="ACHW42_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945307.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHW42_003670"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHW42_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR8945308.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="ACHW42_003671"
     CDS             complement(1254..1670)
                     /locus_tag="ACHW42_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945309.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="ACHW42_003672"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="ACHW42_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2887
                     /locus_tag="ACHW42_003673"
                     /pseudo
     CDS             1837..2887
                     /locus_tag="ACHW42_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2915..3004)
                     /locus_tag="ACHW42_003674"
     tRNA            complement(2915..3004)
                     /locus_tag="ACHW42_003674"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHW42_003675"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHW42_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR8945310.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="ACHW42_003676"
     tRNA            3996..4071
                     /locus_tag="ACHW42_003676"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="ACHW42_003677"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="ACHW42_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1508 a         1326 c         1293 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct
//
LOCUS       DAZSNM010000230         5557 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZSNM010000230 DAZSNM010000000
VERSION     DAZSNM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.230"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..641
                     /locus_tag="ACHW42_003678"
     CDS             <1..641
                     /locus_tag="ACHW42_003678"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HFR8945311.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="ACHW42_003679"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="ACHW42_003679"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR8945312.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="ACHW42_003680"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="ACHW42_003680"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR8945313.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1359 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatcg
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagatatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaa
//
LOCUS       DAZSNM010000231         5554 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZSNM010000231 DAZSNM010000000
VERSION     DAZSNM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.231"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACHW42_003681"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACHW42_003681"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR8945314.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACHW42_003682"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACHW42_003682"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR8945315.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="ACHW42_003683"
     CDS             complement(3121..3420)
                     /locus_tag="ACHW42_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR8945316.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="ACHW42_003684"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="ACHW42_003684"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR8945317.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACHW42_003685"
     CDS             complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACHW42_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFR8945318.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACHW42_003686"
     CDS             complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACHW42_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR8945319.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
BASE COUNT         1346 a         1432 c         1364 g         1412 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atac
//
LOCUS       DAZSNM010000232         5546 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZSNM010000232 DAZSNM010000000
VERSION     DAZSNM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.232"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..694
                     /locus_tag="ACHW42_003687"
     CDS             <1..694
                     /locus_tag="ACHW42_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR8945320.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..1803)
                     /locus_tag="ACHW42_003688"
     CDS             complement(1033..1803)
                     /locus_tag="ACHW42_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945321.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2334)
                     /locus_tag="ACHW42_003689"
                     /pseudo
     CDS             complement(1848..2334)
                     /locus_tag="ACHW42_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(2294..2515)
                     /locus_tag="ACHW42_003690"
     CDS             complement(2294..2515)
                     /locus_tag="ACHW42_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945322.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="ACHW42_003691"
     CDS             complement(2516..3199)
                     /locus_tag="ACHW42_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR8945323.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="ACHW42_003692"
     CDS             complement(3263..3574)
                     /locus_tag="ACHW42_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945324.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3571..4473)
                     /locus_tag="ACHW42_003693"
     CDS             complement(3571..4473)
                     /locus_tag="ACHW42_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR8945325.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..>5546
                     /gene="parM"
                     /locus_tag="ACHW42_003694"
     CDS             4913..>5546
                     /gene="parM"
                     /locus_tag="ACHW42_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HFR8945326.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMN"
BASE COUNT         1408 a         1521 c         1410 g         1207 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggctcac caggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaaatac acctatgatt tgggaagcac
     5101 tgctgtcata ggtacaacac atgtcagtta tcaatacagc accaccaatc tgcttgccat
     5161 acatcatgca ctgctgacat ccggccttca gccacaggat gtagaactga cagtaacatt
     5221 acctgtgacc gaattctttg ataatgacaa tcaaccaaat gaagaaagaa tagaaagaaa
     5281 aaaggcaaat gttctccgtg agatatctct gaataaaggg gaaacattta aaattaaaaa
     5341 agttaatgta atgcctgaat ctttgccagc tgcatttgaa tcactaaaaa aagataaagt
     5401 aaacaaactt gaacgcagcc tgattatcga tcttggagga accactctgg attgtggttt
     5461 aattctcggt gcatttgaag gcatttctga aatccgtggc tattcagaaa tcgggacatc
     5521 ccgcatcact cataccgtca tgaatg
//
LOCUS       DAZSNM010000233         5487 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZSNM010000233 DAZSNM010000000
VERSION     DAZSNM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.233"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1630)
                     /locus_tag="ACHW42_003695"
     CDS             complement(<1..1630)
                     /locus_tag="ACHW42_003695"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR8945327.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2541)
                     /locus_tag="ACHW42_003696"
                     /pseudo
     CDS             complement(1646..2541)
                     /locus_tag="ACHW42_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(2541..3146)
                     /locus_tag="ACHW42_003697"
     CDS             complement(2541..3146)
                     /locus_tag="ACHW42_003697"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR8945328.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5487)
                     /locus_tag="ACHW42_003698"
     CDS             complement(3317..>5487)
                     /locus_tag="ACHW42_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFR8945329.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1582 a         1364 c         1141 g         1400 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgtttat
//
LOCUS       DAZSNM010000234         5409 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZSNM010000234 DAZSNM010000000
VERSION     DAZSNM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.234"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(78..1361)
                     /gene="gltA"
                     /locus_tag="ACHW42_003699"
     CDS             complement(78..1361)
                     /gene="gltA"
                     /locus_tag="ACHW42_003699"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR8945330.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1567..1674)
                     /locus_tag="ACHW42_003700"
     CDS             complement(1567..1674)
                     /locus_tag="ACHW42_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945331.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2055..2459
                     /gene="sdhC"
                     /locus_tag="ACHW42_003701"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2055..2459
                     /gene="sdhC"
                     /locus_tag="ACHW42_003701"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR8945332.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2453..2800
                     /gene="sdhD"
                     /locus_tag="ACHW42_003702"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2453..2800
                     /gene="sdhD"
                     /locus_tag="ACHW42_003702"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR8945333.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2800..4566
                     /gene="sdhA"
                     /locus_tag="ACHW42_003703"
     CDS             2800..4566
                     /gene="sdhA"
                     /locus_tag="ACHW42_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR8945334.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4582..5298
                     /gene="sdhB"
                     /locus_tag="ACHW42_003704"
     CDS             4582..5298
                     /gene="sdhB"
                     /locus_tag="ACHW42_003704"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR8945335.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
BASE COUNT         1281 a         1342 c         1438 g         1348 t
ORIGIN      
        1 ttttgtgttt aggcatataa aaattaaccc gccatttgaa cggcgggtta aaatatttac
       61 aacttagcaa tcaaccatta acgcttgata tcgcttttaa agtcgcgttt ttcatatcct
      121 gtatacagct gacgcggacg ggcaatcttc ataccgtaac tgtgcatttc gctccagtgg
      181 gcgatccagc caacggtacg tgccattgcg aaaatgacgg tgaacatgga agacggaata
      241 cccatcgctt tcaggatgat accagagtag aaatcgacgt tcgggtacag tttcttctcg
      301 ataaagtacg ggtcgttcag cgcgatgttt tccagctcca tagccacttc cagcaggtca
      361 tctttggtgc ccagctcttt cagaacttca tggcaggttt cacgcattac ggtggcgcgc
      421 gggtcgtaat ttttgtacac gcggtgaccg aagcccatca ggcggaaaga atcattttta
      481 tctttcgcac gacgaacaaa ttccggaatg tgtttaacgg agctaatttc ttccagcatt
      541 ttcagcgccg cttcgttagc accaccgtgc gcaggtcccc acagtgaagc aatacctgct
      601 gcgatacagg caaacgggtt cgcccccgaa gagccagcgg tacgcacggt ggaggtagag
      661 gcgttctgtt catggtcagc gtgcaggatc agaatacggt ccatagcacg ttccagaatc
      721 ggattaactt catacggttc gcacggcgtg gagaacatca tattcaggaa gttaccggca
      781 taggagagat cgttgcgcgg ataaacaaat ggctgaccaa tggaatactt gtaacacatc
      841 gcggccatgg tcggcatttt cgacagcagg cggaacgcgg caatttcacg atgacgagga
      901 ttgttaacat ccagcgagtc gtgatagaac gccgccagcg cgccggtaat accacacatg
      961 actgccattg gatgtgagtc gcgacggaaa gcgtggaaca gacgggtaat ctgctcgtgg
     1021 atcatggtat gacgggtcac cgtagtttta aattcgtcat actgttcctg agtcggtttt
     1081 tcaccattca gcaggatgta acaaacttct aggtagttag aatcggtcgc cagctgatcg
     1141 atcgggaaac cgcggtgcag caaaatacct tcatcaccat caataaaagt aattttagat
     1201 tcgcaggatg cggttgaagt gaagcctggg tcaaaggtga acacaccttt tgaaccgaga
     1261 gtacggatat caataacatc ttgacccagc gtgcctttca gcacatccag ttcaacagct
     1321 gtgtccccgt tgaggttgag ttttgctttt gtatcagcca tttaaggtct ccttagcgcc
     1381 ttattgcgta agactgccgg aacttaaatt tgccttcgca catcaacctg gctttacccg
     1441 tttgttattt ggctcgccgc tctgtgaaag aggggaaaac ctgggtacag agctctgggc
     1501 gcttgcaggt aaagaatcca ttgatgacga ataaatggcg aatcaagtac ttagcaatcc
     1561 gaattattaa acttgtctac cactaataac tgtcccgaat gaattggtca atacttccac
     1621 actgttacat aagttaatct taggtgaaat accgacttca taacttttac gcattatatg
     1681 cttttcctgg taatgtttgt aacaactttg ttgaatgatt gtcaaattag atgattaaaa
     1741 attaaataaa tgttgttatc gtgacctgga tcactgttca ggataaaacc cgacaaacta
     1801 tatgtaggtt aattgtaatg attttgtgaa cagcctatac tgccgccagg tctccggaac
     1861 accctgcaat cccgagccac ccagcgttgt aacgtgtcgt tttcgcatct ggaagcagtg
     1921 ttttgcatga cgcgcagtta tggaaaggac gctgtctgac ccgcaagcag accggaggaa
     1981 ggaaatcccg acgtctccag gtaacagaaa gttaacctct gtgcccgtag tccccaggga
     2041 ataataagaa cagcatgtgg gcgttattca tgataagaaa tgtgaaaaaa caaagacctg
     2101 ttaatctgga cctacagacc atccggttcc ccatcacggc gatagcgtcc attctccatc
     2161 gcgtttccgg tgtgatcacc tttgttgcag tgggcatcct gcagtggctt ctgggtacca
     2221 gcctctcttc ccctgaaggt ttcgagcaag cttccgcgat tatgggcagc ttcttcgtca
     2281 aatttatcat gtggggcatc cttaccgctc tggcgtatca cgtcgtcgta ggtattcgcc
     2341 acatgatgat ggattttggc tatctggaag aaacattcga agcgggtaaa cgctccgcca
     2401 aaatctcctt tgttattact gtcgtgcttt cacttctcgc aggagtcctc gtatggtaag
     2461 caacgcctcc gcattaggac gcaatggcgt acatgatttc atcctcgttc gcgctaccgc
     2521 tatcgtcctg acgctctaca tcatttatat ggtcggtttt ttcgccacca gtggcgaact
     2581 gacatatgaa gtctggatcg gtttcttcgc ctctgcgttc accaaagtgt tcaccctgct
     2641 ggcgctgttt tctatcttga ttcatgcctg gatcggcatg tggcaggtgt tgaccgacta
     2701 cgttaaaccg ctggccttgc gcctgatgct gcaactggtg attgtcgttg cactggtggt
     2761 ttacgtgatt tatggattcg ttgtggtgtg gggtgtgtga tgaaattgcc agtcagagaa
     2821 tttgatgcag ttgtgattgg tgccggtggc gcaggtatgc gcgcggcgct gcaaatttcc
     2881 cagagcggcc agacctgtgc cctgctctct aaagtcttcc cgacccgttc ccataccgtt
     2941 tctgcgcaag gcggcattac cgttgcgctg ggtaataccc atgaagataa ctgggaatgg
     3001 catatgtacg acaccgtaaa agggtcggac tatatcggtg accaggacgc gattgaatat
     3061 atgtgtaaaa ccgggccgga agcgattctg gaactcgaac acatgggcct gccgttctcg
     3121 cgtctcgatg atggtcgtat ctatcaacgt ccgtttggcg gtcagtcgaa aaacttcggc
     3181 ggcgagcagg cggcacgcac tgcggcggct gctgaccgta ccggtcacgc actgttgcac
     3241 acgctttatc agcagaacct gaaaaaccac accaccattt tctccgagtg gtatgcgctg
     3301 gatctggtga aaaaccagga tggcgcggtg gtgggttgta ccgcactgtg catcgaaacc
     3361 ggtgaagtgg tttatttcaa agcccgcgct accgtgctgg cgactggcgg agcagggcgt
     3421 atttatcagt ccaccactaa cgcccacatt aacactggcg acggtgtcgg catggctatc
     3481 cgcgcaggcg taccggtgca ggatatggaa atgtggcaat tccacccgac cggcattgcc
     3541 ggtgcgggcg tactggtcac cgaaggttgc cgtggtgaag gcggttatct gctgaacaaa
     3601 catggcgaac gctttatgga gcgttatgcg ccgaacgcca aagacctggc gggccgtgat
     3661 gtggtggcgc gttccatcat gattgaaatc cgtgaaggtc gcggctgtga tggtccgtgg
     3721 gggccacacg cgaaactgaa actcgatcac ctgggtaaag aagttctcga atcccgtctg
     3781 ccgggtatcc tggagctttc ccgtaccttc gctcacgtcg atccggtgaa agagccgatt
     3841 ccggttatcc caacctgtca ctacatgatg ggcggtattc cgaccaaagt taccggtcag
     3901 gcgctgactg tgaatgagaa aggcgaagat gtggttgttc cgggactgtt tgccgttggt
     3961 gaaatcgctt gtgtatcggt acacggcgct aaccgtctgg gcggcaactc gctgctggac
     4021 ctggtggtct ttggtcgcgc ggcaggtctg catctgcaag agtctatcgc cgagcagggc
     4081 gcactgcacg atgccagcga gtctgatgtt gaagcgtctc tggatcgcct gaaccggtgg
     4141 aacaataatc gtaacggtga agatccggtg gcgatccgta aagcgctgca agaatgtatg
     4201 cagcataact tctcggtctt ccgtgaaggt gatgcgatgg cgaaagggct tgagcagttg
     4261 aaagtgatcc gcgagcgtct gaaaaatgcc cgtctggatg acacttccag cgagttcaac
     4321 acccagcgcg ttgagtgcct ggaactggat aacctgatgg aaacggcgta tgcaacggct
     4381 gtttctgcca acttccgtac cgaaagccgt ggcgcacata gccgcttcga cttcccggat
     4441 cgtgatgatg aaaactggct gtgccactcc ctgtatctgc ctgagtcgga atccatgacg
     4501 cgccgaagcg tcaacatgga accgaaactg cgcccggcat tcccgccgaa gattcgtact
     4561 tactaatgcg gagacaggaa aatgagactc gagttttcaa tttatcgcta taacccggat
     4621 gttgatgatg ctccgcgtat gcaggattac accctggaag cggaagaagg tcgcgacatg
     4681 atgctgctgg atgcgcttat ccagctgaaa gagaaagatc ccagcctgtc gttccgccgc
     4741 tcctgccgtg aaggtgtgtg cggttccgac ggtctgaaca tgaacggcaa gaatggtctg
     4801 gcctgtatta ccccgatttc ggcactcaac cagccgggca agaagattgt gattcgcccg
     4861 ctgccaggtt taccggtgat ccgcgatttg gtggtagaca tgggacaatt ctatgcgcaa
     4921 tatgagaaaa ttaagcctta cctgttgaat aatggacaaa atccgccagc tcgcgagcat
     4981 ttacagatgc cagagcagcg cgaaaaactc gacgggctgt atgaatgtat tctctgcgca
     5041 tgttgttcaa cctcttgtcc gtctttctgg tggaatcccg ataagtttat cggcccggca
     5101 ggcttgttag cggcatatcg tttcctgatc gatagccgtg ataccgagac tgacagccgc
     5161 ctcgacggtt tgagcgatgc attcagcgta ttccgctgtc acagcatcat gaactgcgtc
     5221 agtgtatgtc cgaaggggct gaacccgacg cgcgccatcg gccatatcaa gtcgatgttg
     5281 ttgcaacgta atgcgtaaac tgtaggcctg ataagacgcg caagcgttgc atcaggcaac
     5341 cagtgccgga tgcggcgtga acgccttatc cggcctacaa gtcattaccc gcaggcctga
     5401 taagcgcag
//
LOCUS       DAZSNM010000235         5399 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZSNM010000235 DAZSNM010000000
VERSION     DAZSNM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.235"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..497
                     /locus_tag="ACHW42_003705"
     CDS             <1..497
                     /locus_tag="ACHW42_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HFR8945336.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="ACHW42_003706"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="ACHW42_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="ACHW42_003707"
     CDS             2500..3114
                     /locus_tag="ACHW42_003707"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR8945337.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="ACHW42_003708"
     CDS             3114..3443
                     /locus_tag="ACHW42_003708"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR8945338.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHW42_003709"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHW42_003709"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR8945339.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="ACHW42_003710"
     CDS             4529..4840
                     /locus_tag="ACHW42_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR8945340.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..5373
                     /locus_tag="ACHW42_003711"
                     /pseudo
     CDS             4824..5373
                     /locus_tag="ACHW42_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1178 a         1344 c         1425 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggcaa
//
LOCUS       DAZSNM010000236         5355 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZSNM010000236 DAZSNM010000000
VERSION     DAZSNM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.236"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..171
                     /locus_tag="ACHW42_003712"
     CDS             <1..171
                     /locus_tag="ACHW42_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8945341.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="ACHW42_003713"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="ACHW42_003713"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR8945342.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="ACHW42_003714"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="ACHW42_003714"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR8945343.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHW42_003715"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHW42_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR8945344.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="ACHW42_003716"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="ACHW42_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR8945345.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT         1321 a         1189 c         1402 g         1443 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta tttattttaa aatggttaag ttttgctaat catgatgagt
     5341 tatttttgat agttc
//
LOCUS       DAZSNM010000237         5337 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZSNM010000237 DAZSNM010000000
VERSION     DAZSNM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.237"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHW42_003717"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHW42_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR8945346.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACHW42_003718"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACHW42_003718"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR8945347.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHW42_003719"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHW42_003719"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR8945348.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACHW42_003720"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACHW42_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR8945349.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1289 c         1457 g         1281 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcaacag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggg
//
LOCUS       DAZSNM010000238         5164 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZSNM010000238 DAZSNM010000000
VERSION     DAZSNM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.238"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..222
                     /locus_tag="ACHW42_003721"
     CDS             <1..222
                     /locus_tag="ACHW42_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8945350.1"
                     /translation="SLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFH
                     WGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(394..723)
                     /locus_tag="ACHW42_003722"
     CDS             complement(394..723)
                     /locus_tag="ACHW42_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR8945351.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(728..913)
                     /locus_tag="ACHW42_003723"
     CDS             complement(728..913)
                     /locus_tag="ACHW42_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR8945352.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(910..3549)
                     /locus_tag="ACHW42_003724"
     CDS             complement(910..3549)
                     /locus_tag="ACHW42_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR8945353.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3757..4746
                     /gene="ldhA"
                     /locus_tag="ACHW42_003725"
     CDS             3757..4746
                     /gene="ldhA"
                     /locus_tag="ACHW42_003725"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR8945354.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4857..>5164
                     /gene="hslJ"
                     /locus_tag="ACHW42_003726"
     CDS             4857..>5164
                     /gene="hslJ"
                     /locus_tag="ACHW42_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR8945355.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     "
BASE COUNT         1308 a         1322 c         1204 g         1330 t
ORIGIN      
        1 agtttgtacc gaatgtttgc cgataaaggt ttggtagtcg cgcaatatat tcgtaaccgt
       61 cgtctcgatt tttgtgcaga tgcgattcgc catgccgcag atgatgaaaa actggcaggc
      121 atcggctttc attggggatt ttctgaccag agtcattttt caacggtatt taagcaacgc
      181 tttgggatga cgccaggcga gtatcgacgt aaattccgct aactaattgg cgttgcatgt
      241 actgcaacgc caattagtta aaacagatat ttgactatgc atttttcagg gcgaaaggca
      301 atttgacagg agtttttgaa tattcacctt gtttcaggaa aatagatcaa tcgctttctc
      361 ctgtagcgtt atcccgatat tgcggttttg ctattaaaac tttcgcaccc atttaccgtt
      421 tatcccttgt acatattccc ccggtttggc ccgagctacg agcttttgcc cggcgagttt
      481 cgcgatatca tctacagaca cattattttg ctttgcccgt tgttggtaac tcgctttacg
      541 ttcggcatta atttcggtta ctaatttctc tgtttcagca tccgttttca gcgcaaccag
      601 ataaccataa aatgtttcac ctacccgccc ctgagttctg gcttcatcca gagtcaatgc
      661 cattacattg ctgcttacca gcccaacaag aaacgcacaa agaagtaatg ttttcttcat
      721 catcgcctca gaaaagatcg ctacgagttt caagcagctc ttcgacatct ttgtctgcct
      781 tgatgatgat ctcatgctca attttaacgt tcatattgat agtgatcggt tccttaggtg
      841 cagcgacttc aatgcgaggc gtacagccaa ccagcataaa agatgacgtc aacgcagcca
      901 gtaaaatttt cattgttttt cctcacactc ttttccttgc ggacagtcat ttcccggcag
      961 acgtgcgttc tgctccaacc atgcctggag attatcgcca aagcgtaaac tgcgccacag
     1021 cgtaaaaata ttctcttcat gatggtaatt aaggtttacc gtaccgcttt taccatcaac
     1081 gcgactggta cctgtaatgt tggcctgcat ggttaataaa ccgagattat ctaagttaat
     1141 ttttgtcgat gaccggctaa tttccatata gcgcaaccag ttaattgctg aacccgcagt
     1201 catattgtct ttcaccaccg catctgcggt atctttatcc agccgcaagg tcattagccc
     1261 gctatttgcc agccaaccat ctttcactat ccatttttcg ttgtttagcc ataacggcaa
     1321 tgcaccacta aatgccccgg acatggcgaa ttgtttcgga ttgacggcgc taaccagttc
     1381 gctggatgag agattattca gccgcaacag cgccgggtca tgttgcggca tacgtaattg
     1441 tttcatcagc acgttaccgc ctaacacatc gacgctaaca tcagtcaaca gcaagggttc
     1501 ttcttcagtc cacggataac gcccttgcaa atcagccgta atatttttcg ctgtcaccag
     1561 attaatcact tcggcaattc gcaacgtaac ggggccgcga gtccccagat gccaggctcc
     1621 atcggcaaaa cggaaaggca ggacaaaatc gacaccgtta acctgattat ctggcatcca
     1681 ggcactaccg cctttcaaca cgccgtgccc tcccgcgcgg aatccttgtt caggcgcagc
     1741 agaaaatgca acctgagcat acagttcacc atcgcgtaag ttcatcttcc agtcgggtgg
     1801 caccagcggc tggaatacag tcagtgattg ttttggccac caggcgttac cgcgcagacg
     1861 aataccgtcc cagcgaccat ttacccgaac cgggccaatc tcaccagcat gtaaatcgcc
     1921 tttaaagagg aaataggtcg gattgcgccc atcgacgcta aattttaagg ttgatggcgg
     1981 taacacactg ccgccagtga acagcgtttg cccggcgtcc agtgacagcg cgccgctgaa
     2041 ggagggatgc tgtgcgtcac gtacccagac gacgggcttg tcgagaatta atcgcggctt
     2101 ttctaccgtc atcgtaccgt attgtaactg gtcgaaaccg gtggagagat cggtcagcgt
     2161 aatcgtgctg tcatgccact caccttttcc tgcgacatcc caggtggcat tcatcggtgt
     2221 aaaactccct tttccccagt agcgccactg ccagcggcca gcgtcaggga gaaaatcatt
     2281 cgccagccca tccatatgca gcacgaaatc gcccagttca ttttcatgcg cctgcaaaat
     2341 ggcctgcaaa cgtccgtcaa caccacgttg ggtgactttt acacccgcta aaggccagcg
     2401 gatttcatcg atgtccagcg aatcgatgac tcttcccttt gaacgaagta acgcgcctgg
     2461 ctcaaaggtc agcgttgggt cagacagact tccacttagc tgcgcaggta aacgggcata
     2521 taaaatgagg tccgcctgtt tcgcttcacc ggtcagctgc agaggcagtt gactgttatc
     2581 catgcttaat tttcctgggc caaaattaag caccgcgttg cccttacccg cttgcccctg
     2641 ggtcagcaca ttcagtcgtc cgctgaccag agcgttctca agccctgcct gccagttgtc
     2701 gactttgaca cccagtcggc cactcaaagg aaaaccagca tacagccagc tccagcgacc
     2761 atcgcttacg gtcaattgtt gacgagtaat ttgccacggc aaatcgagca acggatcgcc
     2821 gttatcccgt gccagcacga tcaattgccc gctattttcc tgccagtcca gctcggcatc
     2881 caccagtgac ggttcctgcg gcaagtttaa cgtcgcagta gcatgcccac ttacaggaag
     2941 tccatccggc acgagcggca tagtaaattc ccccaccagt ttaaccggcg gctgattttc
     3001 aaacgcgacg acatccagtt cgctgactgt aagttgttgc cctttcagct ggccttgaaa
     3061 tttaactttt tcgccctgat aacgcagttg ctggatatca gaggttaatg cgagagagag
     3121 ttttccctgc cattcctgcc agggagaaaa aatcagttta tcgatattga tccaggtgtt
     3181 aggcagcatg gactgccact gcgcgagggt ttttggagca gctggcgatt gctccgtctg
     3241 cggcaatttc gccagacaag cagaatcaag ttctaccgtg ccgacgttca ataaccagcg
     3301 gctgggatgt gaaaggctgg cgttggtgat atgcgcaagc tgacaatctc ccaccagata
     3361 acggagatcg ggaatgatta aacctttacg cgtaatgcgt gggctttcat ctaatgcaat
     3421 acgtgtcccg agcggtagcc agatgtccgc cagcgtggga acccacagcc cgagcgtcat
     3481 cagcagcgtc aacggcacaa gaataatcag taataacagc gcgagaacgg ctttatattt
     3541 acccagcatg ggtagttaat atcctgattt agcgaaaaat taagcattca atacgggtat
     3601 tgtggcatgt ttaaccgttc agttgaaggt tgcgcctaca ctaagcatag ttgttgatga
     3661 atttttcaat atcgccatgg ctttcaatta tatttgaaat tttgtaaaat atttttagta
     3721 gcttaaatgt gattcaacat cactggagaa agtcttatga aactcgccgt ttatagcaca
     3781 aaacagtacg acaagaagta cctgcaacag gtgaacgagt cctttggctt tgagctggaa
     3841 ttttttgact ttctgctgac ggaaaaaacc gctaaaactg ccaatggctg cgaagcggta
     3901 tgtattttcg taaacgatga cggcagccac ccggtgctgg aagagctgaa aaagcacggc
     3961 gttaaatata tcgccctgcg ctgtgccggt ttcaataacg tcgaccttga tgcggcaaaa
     4021 gaactggggc tgaaagtagt ccgtgttcca gcctatgatc cagaggccgt tgctgaacac
     4081 gccatcggta tgatgatgac gctgaaccgc cgtattcacc gcgcgtatca gcgtacccgt
     4141 gacgctaact tctctctgga aggtctgacc ggctttacta tgtatggcaa aacggcaggc
     4201 gttatcggta ccggtaaaat cggtgtggcg atgctgcgca ttctgaaagg ttttggtatg
     4261 cgtctgctgg cgttcgatcc gtatccaagt gcagcggcgc tggaactcgg tgtggagtat
     4321 gtcgatctgc caaccctgtt ctctgaatca gacgttatct ctctgcactg cccgctgaca
     4381 ccggaaaact accatctgtt gaacgaagcc gccttcgatc agatgaaaaa tggcgtgatg
     4441 atcgtcaata ccagtcgcgg tgcattgatt gattctcagg cggcaattga agcgctgaaa
     4501 aatcagaaaa ttggttcgtt gggtatggac gtctatgaga acgaacgcga tctgttcttt
     4561 gaagataaat ccaacgacgt gatccaggat gacgtattcc gtcgcctgtc tgcctgccac
     4621 aacgtgctgt ttaccgggca ccaggcattc ctgacagcag aagctctgac cagtatttct
     4681 cagactacgc tgcaaaactt aagcaatctg gaaaaaggtg aaacctgccc gaacgaactg
     4741 gtttaatctt gcagctcccc tgcaatccag gggagctgat tcagataatc cccaatgacc
     4801 tttcattctc tattcttaaa atagtcctga gtcagaaact gtaattgaga accacaatga
     4861 agaaagtagc cgcgtttgtt gcgctaagcc tgctgatggc gggatgtgta agtaatgaca
     4921 aaattgctgt tacgccagaa cagctacagc atcatcgctt tgtgctggaa agcgtaaacg
     4981 gtaagcccgt gaccagcgat aaaaatccgc cagaaatcag ctttggtgaa aaaatgatga
     5041 tttccggcag catgtgtaac cgctttagcg gtgaaggcaa actgtctaat ggtgaactga
     5101 cagccaaagg gctggcaatg acccgtatga tgtgcgctaa cccgcagctt aatgaactcg
     5161 ataa
//
LOCUS       DAZSNM010000239         5161 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZSNM010000239 DAZSNM010000000
VERSION     DAZSNM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.239"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..244
                     /locus_tag="ACHW42_003727"
     CDS             <1..244
                     /locus_tag="ACHW42_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945356.1"
                     /translation="EMLSFLTSEEREALTNMLARKGVETVLRLLDKETQELLQLEGRK
                     RLAALMLVDMPDEQVREILEEIEASILSESVNAKAG"
     gene            493..1437
                     /gene="tauA"
                     /locus_tag="ACHW42_003728"
                     /pseudo
     CDS             493..1437
                     /gene="tauA"
                     /locus_tag="ACHW42_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="ACHW42_003729"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="ACHW42_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR8945357.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="ACHW42_003730"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="ACHW42_003730"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR8945358.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="ACHW42_003731"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="ACHW42_003731"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR8945359.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHW42_003732"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHW42_003732"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR8945360.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1181 a         1304 c         1469 g         1207 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataatt tcatattcac tgaaaatatt tttaatcttt atttatagct cttggttatt
     5161 a
//
LOCUS       DAZSNM010000240         5151 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZSNM010000240 DAZSNM010000000
VERSION     DAZSNM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.240"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            190..447
                     /locus_tag="ACHW42_003733"
                     /pseudo
     CDS             190..447
                     /locus_tag="ACHW42_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="ACHW42_003734"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="ACHW42_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR8945361.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="ACHW42_003735"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="ACHW42_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR8945362.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="ACHW42_003736"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="ACHW42_003736"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR8945363.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="ACHW42_003737"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="ACHW42_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3215..3431
                     /locus_tag="ACHW42_003738"
                     /pseudo
     CDS             3215..3431
                     /locus_tag="ACHW42_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(3605..4420)
                     /locus_tag="ACHW42_003739"
     CDS             complement(3605..4420)
                     /locus_tag="ACHW42_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8945364.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTCYSPGKFTRRLTMHNYAITRQMI"
     gene            4517..>5151
                     /locus_tag="ACHW42_003740"
     CDS             4517..>5151
                     /locus_tag="ACHW42_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR8945365.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1375 a         1174 c         1226 g         1376 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcacgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaatataccc t
//
LOCUS       DAZSNM010000241         5122 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZSNM010000241 DAZSNM010000000
VERSION     DAZSNM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.241"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(118..436)
                     /locus_tag="ACHW42_003741"
                     /pseudo
     CDS             complement(118..436)
                     /locus_tag="ACHW42_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(580..3888)
                     /gene="icsA"
                     /locus_tag="ACHW42_003742"
                     /gene_synonym="virG"
     CDS             complement(580..3888)
                     /gene="icsA"
                     /locus_tag="ACHW42_003742"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFR8945366.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4417..>5122
                     /gene="virA"
                     /locus_tag="ACHW42_003743"
     CDS             4417..>5122
                     /gene="virA"
                     /locus_tag="ACHW42_003743"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR8945367.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLS"
BASE COUNT         1565 a         1158 c          856 g         1543 t
ORIGIN      
        1 ttcaggctgt acgcttcccc atgtgatttg cctcgccact tcaccgggct caggaccccg
       61 taggatcttc accgaaaaaa atgcatcacc agttgcttta ttatttgttt taggtagacc
      121 tgttactaat ttatacctac tctgaccaag tctggataag aaaggcacac ctgcacattt
      181 ctacttggaa atgttttctt ttaccatttt gctcaaaaat ttcaacatcc aatattcctc
      241 gtacagaact actcaagtcc tgaatagaaa aagttccagc tggtacagta gagtcatata
      301 ttactcgacc ttgttgagat acaacaacac gtgcattagt ttctgcaata ccagtaatct
      361 gaggcgcata accacgaata tttggcggta gcattcgttc atcgctttct aatgcaattc
      421 ctgtgtaacg ccaagcaaca gcgcattctg gcgcatgatc tggtaaacac gtttggcatt
      481 gatcgcaggc ataccatcat gtgcacaaaa acccaccgag cttttacacg gtgggtccca
      541 gagaaatgca ggacatcaac acgccctgca tttttattat cagaaggtat atttcacacc
      601 caaaatacct tgggtgtctc tgtaactgtt attacccaat tgctgagata cgtttcccca
      661 gatgcttagg ttatctgcaa gacgcccccc cacaccagtt ttgagttcaa tcacattacg
      721 gttgcctatc tggtgatttg acatgccatt cataattaca ccatattgct ttgagctcca
      781 tttccaattc acttccaaaa atggttcgaa cttacgaaga gtctccttat cgatacaact
      841 ctgactaatt aaggatgtac gcaatcccaa ttttgcttga atattattgc ccccagagga
      901 tgtgaccata gtgccattgt gttcaatata gtcgtttgct tttaccccat gcctagttat
      961 ctggacttgt ggattgagat acaatgcgtt gtcaatatta ttatgagcag tccagcgtat
     1021 ggttgggtag atatagccag cttccaaagc tcctgttatt cctttgctgg aatatttctc
     1081 accagttaac ccatctcctt tgactgtatt attaaaccaa ctgtactgca accaagcatc
     1141 catatataga cctgttcgtt ctttttcatc ctgaaaccaa gaactgtaca aaccggcagt
     1201 ataaccgctc acagttcctc gtgaactata actagtatac ttattatgag ttttgctatc
     1261 ctgatatcca taagctccca taatacccac atgcaagcca tcatgatcag tgaagtttgt
     1321 cttcaaaata tctccaccaa tctgataaac catcctgtta gctgtggttt ttaattgtct
     1381 atcagctaaa ttatgctcat tatgtccacc aacagtacga atccacatgg tggtttcgct
     1441 gagctgacct gtagtaggat caacaatagt gctaccgtgt cggtcataga gagaaggact
     1501 aaacatcgtg ttcattgcta tcaggttagc aatataactg ccagcttcag ggcgccatgt
     1561 gtgaatacta ccttcaccca aattaagtga agatacaagc tgtgaactag ctcggcgact
     1621 actcatttga gtagactctt gattgctcat ctgttttgat tcttgattat ccatctgact
     1681 agttagatac cacttattgg tattcagtcc agatacagtg ccctgtttca ggcgatagtc
     1741 ataactacca gcaacaatac ggcctttctg aataaaagca ttcttatcag aagaatctgt
     1801 cgaaataatt tgaacacctt caagagtttt atctccagag cctccctcat ttgtcactgc
     1861 aacataggta ataccagacg catggcctgc aatttccagc cggtcagttt tactgttgtc
     1921 attgccgaga accgcgccca aagataatat tccgcccttt ccatgccaat tacctttctg
     1981 aacataggtc tgaccgacat ttgacgaact attattgaga ataacatgac cgtttttctc
     2041 cagagtcatg ttacctgtga caatgacggg gtcaggcaaa aaaggggcat taatattatt
     2101 gattagcaca gtcccactat ttaataaagt gttaacagtt tggttcatgc ctgaaaaatt
     2161 caaggtagca cctttattaa caataacacc agcggtacgt gtcatagctt caactgtccc
     2221 catttttaga atgcctgcat tgatgttggt gaaaccagag taggtatttt tttccgccaa
     2281 aatgagagtt ccctcccctg attttgttag ggattttccg tcccagctat ttatattatg
     2341 atggctgaga ttatctgcca gaatagtact aacagtaagt tcagcgtttt ctttcagatt
     2401 gaactctcca tgactgtcgc catctgtatc attccagctc aaaccgtaga tcaatttatt
     2461 ctggtcactg atatagcttt ttgtatagtc aatagcacca gtagtgagta acggattgat
     2521 gatagatata ttagaaacac tgaaacctgt tatcccttta tctgcactta gcagtttata
     2581 gtccttctgc gcccagttat ttataatagc ctccactttt acattgttgc caaatgacat
     2641 atttccagaa gcttccagac tactgattcc agctaaattt aaagaactgt tctcaccaaa
     2701 agaaacgtca cctgaaacag tgaatttgtt acctgatagt gaaacagttg tacaatcacc
     2761 agcacataaa tcaccttcaa tagttggtgt ggtcccagtg taatcattgt tgataatttt
     2821 cagaatggat gaattgttaa cctgtacatc accagtaatg acagaacctt catggagtat
     2881 taatgaattg tttccaccag tgatgtggat agcattacct gctacatttt gtgttccatc
     2941 atcttcttta cctgaaatta tatatccgct attagcaatg gtcatattgc taccatttat
     3001 agcatccccc caatgtccac cattaccgcc taaaatagct ccattgttga ttacagacag
     3061 gttatctcct gtgatagcat taccaccgta gccatcataa ccataaccgt aagcagcacc
     3121 tccgttgcct ccagcgaacg tgcccttatt gattacagag aggctgcttc ctgtgatggc
     3181 atcacctcca ttaccgccat caccctcacc ataattgtta cctccattac caccggaaat
     3241 agtccctttg ttgattatgg gtaaattgac tcctgtgacg gcatcacctc cattcccccc
     3301 atcactatca ctataactat cgccaccatg tccacctgaa ataatttctc catttattat
     3361 aaacagattg tctcctgtaa cagcctcacc accatcaccg ttatggtcag caccgctacc
     3421 gccgctacca ccaagaatca tgccttgatt gattatagac aagtcagatc cggtaataga
     3481 gtcaccacca ttaccgccac aactattacc atcattatta tcaccagcac caccatgacc
     3541 tgcgctaatc ataatatcag agttaatccg tagcttagtt ctagatgcat gagaggggtt
     3601 ggaagaagtt atataccacg catccattcc atcttcacca gctcccaagg gagaaagtcc
     3661 atcaacaggt gttaataatt tttcataatt gtcctctgaa aaatgaagtt cttgagtacc
     3721 cgaaagagga gtagcaaaag ctattggccc cccgagcaac agggatgcac caacaacaaa
     3781 aggagaaaaa gacaatttgc atgttttttc ctttaccgta ggtaattctc cggccccccc
     3841 ctgtgaacat tgggtcatat tacaaaaaaa tttgtgaatt tgattcatgc actatattat
     3901 cagtaagtgg ttgataaacc cctgaaaaga ttccgtttta tccggaataa agggacgata
     3961 tatgcaaaaa catattaaac aaagcctcaa ccaaaacatg agagctgtgt tatatatcaa
     4021 tgcaacggga gaaatttacc tgtgtcttgc tttgaaaaaa agagggggaa agctatatgt
     4081 tcgataataa attaataatt ttactatgat agtctgcttc agaccgcaac ttttcagttc
     4141 tttctgtacg cttgcccaaa gtttaaccac tttgtttttc tatacaccca atggttctat
     4201 ctgccccttg atgttaatcc ccaaagccaa taacatgaat gtattcatat cacaactttc
     4261 ctaccagact ctccaatgca ataactgcat aattgttttt agtggaaatg tatcgttttc
     4321 ttaaagagaa gaataacatt ccattttatt atgttcccaa tttaattata ttaatatata
     4381 ttggcattaa taggaaaata catcaggaga aatcaaatgc agacatcaaa cataactaac
     4441 catgaaagaa atgactcatc atggatgtca actgtcaaat caactacaga agtaagttgg
     4501 aataaattga gtttttgtga cattttactg aaaataataa ctttcggcat atattctcca
     4561 cacgaaactc tagccgaaaa acatagcgaa aaaaaactaa tggatagttt ctcaccatca
     4621 ctatcacaag ataaaatgga cggagagttt gcccacgcaa acatagatgg catttcaata
     4681 cggctatgcc ttaacaaagg catatgttcg gttttttatc ttgatggaga taaaatacaa
     4741 tccactcaac tttcatcaaa agaatataat aacttgttaa gttcactccc ccccaaacaa
     4801 ttcaacttag gaaaagtaca tactattaca gctccggtat cagggaattt taaaactcac
     4861 aagcctgcac cagaagttat tgaaacagcc attaattgct gtacatcgat aataccaaac
     4921 gatgattact tccacgttaa agacactgat tttaatagtg tttggcatga tatttacaga
     4981 gatattcgag catctgacag caatagtaca aaaatatatt tcaacaatat tgaaatacca
     5041 ctaaaattaa ttgcagatct aattaatgag ttaggaatta atgaattcat tgatagcaaa
     5101 aaagagcttc aaatgctaag tt
//
LOCUS       DAZSNM010000242         5098 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZSNM010000242 DAZSNM010000000
VERSION     DAZSNM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.242"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            191..250
                     /gene="ypfM"
                     /locus_tag="ACHW42_003744"
     CDS             191..250
                     /gene="ypfM"
                     /locus_tag="ACHW42_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR8945368.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(349..3462)
                     /gene="acrD"
                     /locus_tag="ACHW42_003745"
     CDS             complement(349..3462)
                     /gene="acrD"
                     /locus_tag="ACHW42_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR8945369.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(3664..5098)
                     /gene="narQ"
                     /locus_tag="ACHW42_003746"
                     /pseudo
     CDS             complement(3664..5098)
                     /gene="narQ"
                     /locus_tag="ACHW42_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
BASE COUNT         1247 a         1378 c         1288 g         1185 t
ORIGIN      
        1 taaatcagta ttagatgccg tatgaatatc tcgaaatgtt gctgtgtatc acatacaaat
       61 tatcaccgca ggaccatagt atttacataa ggtttgtccc ccggaggtag tcatcgcagc
      121 aatcgagtaa attagcttca ccacggtata ttgtttccgt agaataccca taattataag
      181 agaggttgtt atgattgaac gtgaactggg gaactggaaa gactttatcg aagttatgct
      241 tcgtaagtaa ttcctgagac aaagtataaa cagcaagaac ccgctgagca ggttcttaat
      301 cggaaagttg aaaaaggcga cacattggca tgtcgccttt tttattgctt attccgggcg
      361 cggcttcagc gggaagcggc ggcgcaccag cacaaagaac agcggcacga agtaaatagc
      421 cagaatagta gctgaaatca tcccgcccat tacgccagta cccaccgcat gctgaccacc
      481 ggaaccggca cccgtgctgg ttgccattgg caatacgccg aagataaatg ccagcgaggt
      541 catcagaatc gggcgtaaac gctgacggca ggcgtggagc gtcgcttcaa acaggtcatg
      601 gcctttttgg ttcatctcgt tagcaaactc gacgatcagg atggcgtttt tcgccgataa
      661 accaatgacc gttaacaggc ccacctggaa gtaaacgtcg ttttccagcc cgcgcatcca
      721 ggttgccagc agcgcgccga ttacccccag cggcacgacc agcattaccg agaacggtac
      781 cgaccagctt tcatacaatg ccgccagaca caggaatacc accagcaagg aaatggcgta
      841 cagcgccgga gcctgcgcgc cggaaagccg ctcctgatac gacatcgccg tccactccag
      901 accaaagccg tttggcagct gcttcactaa cgattccata atatccatcg ccgtaccggt
      961 actgaccccc ggtgcggctt ccccaacaat ctcaaccgca gaataaccgt tatagcgttc
     1021 cagacgcggc gagcctgttt cccagcgtga ggtcgcgaaa gcagagaagg gcaccatgcc
     1081 gccatcttta tttcggacat accagagatt gatgtcatct ggcagcatgc gatacggcgc
     1141 agctgcctgc acatagactt tcttcacgcg accgcgatcc ataaagtcat tcacatagct
     1201 cgaaccccag gcggtttgca gtgtgtcgtt aatatcgtcg atatcaacgc ccagcgcctg
     1261 agctttacgc tggtcgatat caatctgcaa ctgcggactg tcatcgaggc cgttatggcg
     1321 cacacgggtt agctccgggt tttccgccgc cagcgccagc aactgattac gtgctgccat
     1381 cagcgcatcg tgacccgctc cagcgtggtc ctgcaactcc atatcaaaac ctgcagaact
     1441 accaagaccg ctaattgctg gtgggctgct ggcgataacg cgagcttctt taatttggtt
     1501 aaacgccttc gttgcacgct cgataatggc aaacgaggtg ccggtcttac tgtcgcgttc
     1561 gctccagtct ttcaggcgga taaacattct cgccacgttt tgcccgttac ccccagggcc
     1621 agaaccaacg gtggcaaaca ccgacatgat gttgtctttt tcatgggtga agtaatattt
     1681 ctcgatttgc tcaacgactt tcagggtctg ttgttgggtt gaaccgctgg gcaattgtac
     1741 cgaggtagta aacatgcccc ggtcttccag cggtaagaac gacgtcggca aacgcaggaa
     1801 caggaacacc atgccgccaa gcagcaggac ataaatcaca atccagcgca ggctacggtg
     1861 gagaattttc gccacccctt tttcgtagcg ttcggcgttg cggttaaaca tctggttaaa
     1921 ccaggcaaaa aaaccttttt gcccgtgatg ctcacctttt ttcagtggct taagtagcgt
     1981 ggcacacaga gccggagtga ggagcatcgc taccagtact gacagcacca tcgccgcaac
     2041 aatggtaata gagaactggc gatagatggc accggtggtg ccgccgaaga aggccattgg
     2101 tacaaatacc gccgaaagca ccatcgcaat cccgaccagt gccccctgga tctgccccat
     2161 cgatttacgt gtggcttcgc gaggagtgag tccttcctca ctcataatac gttcgacgtt
     2221 ttccaccacc acgatggcgt catccaccag cagaccgatc gccagcacca tcgcgaacat
     2281 ggttaaggtg ttgacgctgt aaccgaaggc gtaaagtacg gagaaggttc ccatcaacac
     2341 caccggcacg gcgatagtgg gtatcagcgt ggcgcggaag ttttgcagga acagatacat
     2401 aacgaggaaa accagagcga tagcttccag cagcgttttc accacgtctt caatggaggc
     2461 tttaacaaac gaggtggttt catacgccac cttgtattcc agtccgtgcg ggaaatactg
     2521 cgccagctcg tcgagacgat tgagcaccag ctccgctgtc gccatttcgt ttgcgctgga
     2581 ggccagtttt acccccaacc cggaggctgg cttaccattg aagcggctaa gataatcgta
     2641 tttctccgcc cccatttcga cggtggcgac atcgcccagc cttacctctg agccgtcctg
     2701 attcacccgc aaggtgatat cgcggaactg ttctggtgtt tgcagcagtg actgggcgtt
     2761 aatggtggcg ttgagcgcct gcttatcgac ggaaggtgta ccaccaagtt gcccaaccgc
     2821 aatctgcgcg ttctgtgact caatggcatc agtgacatct ttagccgtca tctggaaact
     2881 gttgagtttc gccgggtcca gccagatacg catggaatat tgcgaaccat aggcatcgat
     2941 atccccgacg ccattcacgc ggcttaacgg gtcctgaata ttactggcaa cataatcagc
     3001 aatatcctgt ttatccatcg agccatcggt agagacgaag gcaatggtca gaatgttggt
     3061 atcgccggtt ttacgcaccg tcacgccctg attttgcacc gcctgcggta actttcgcat
     3121 ggctgattgc agctggtttt gtacttgctg cacggcttcg tccggatcgg tgcctgcttt
     3181 aaaacttaaa gtgacagatg cctgaccggt gccactgctc tgagatgaca tatacatcag
     3241 attatcgagg ccggtcatat tttgctcgat aacctgggtc acggtgtttt ccagcgtctg
     3301 ggccgatgcg ccgggatagt tagcggtcac tcgcacattc ggtggcgcga gatcggggta
     3361 ttgttcaacg ggcaatgaaa aaatcgccag ggtacctgtc agacacaaca ggattgtcag
     3421 cacccaggca aaaatggggc gatcaataaa gaaattcgcc attaaaagag gacctcgtgt
     3481 ttctgcgtat tgttgtaggc ttgacttgct tcactgtagc ggcaaggtac gagccaatcg
     3541 tggagaaaaa aaggagttaa tgtaaattat catgagattg ctgacacact cgcttacagg
     3601 tcaatatggg cacgtttttc cctctcgcct ttaaagggag agggccggtg tgagggtaat
     3661 tatttacacc tcagccggcg tgttcccctt cccctcaccc tctgcggagc gaaagctaat
     3721 actcactaac gtgccgccgc cggaaggttg tgaaaaagtc agcgtcccgc ccagccgttc
     3781 cgcgcgttcg cgcatgatat tcagtccata atgaccttcg ggttctttcg gttcaccgat
     3841 accaatcccg ttatcacgga tataaaccgt gtgattgccg tccggcgcgg tgacgcaact
     3901 gacggcgatt tcgctggcgt tggcgtgctt catcgcattc agcaccgctt cgcgaataat
     3961 ttgcaacaaa tgcacctgca tttgcgcatc cagtgccagg gttggcagac ggcagtcgag
     4021 ggtcagttta gcgctggttt gattttgtaa cgtatccagc atttccctca acgcggaggg
     4081 gagatccgcc tgctgcagcg tcaggcggaa agtagtcaac agctcgcgta actgccgata
     4141 agcatcattc aacgcctggg aaaaatcggc catgatactt tgtgcggtgg cgttatcttc
     4201 cggtatcgaa cgcttcagta acgtcaactg gatacgtaag taagaaagca cctgagccag
     4261 cgagtcgtgc aattcgcggg cgatggtcgc acgttcttcc atcaacaata actgctgaaa
     4321 atgcttctgc gcctgattaa agtacaaacc gcgtcccagc atcgatgaaa cactgttaag
     4381 cagcggttct gatgatgaaa cgtgactatt ttgccagtgc agttcgccgt aaaccgtctc
     4441 ttgcattgtc accggtaaaa tctgcattgg cagctcaggg tttggcttcc cttcgctaat
     4501 ccgccagtta tccccaacgt tcagctccag atattcagct gcttcattgt cgcgaacaat
     4561 ctgcaaaata tggcggaaac aatgcacatc aatctggctg gtgttcagcg cctgggaaca
     4621 ctgcactgat acaacacctc cagacgacgc ttggtctcgt ggagatcgcg ggtcttttct
     4681 tctactgacg cctccagcga aaggtacaat ttatgcagct cgctcgacat ttggttaaag
     4741 gtttttgcaa gcagaccaag ctcattcggc agactggtat ccagcggcgg cgagtcgaac
     4801 tgcccgtgtt caatacgctg actggcggta accagctgat tcagcggggc aaccacctga
     4861 tggcgtatgc ggcgcaaagt aaaaaagacc agcgtgaaaa taccgatacc gccagccagg
     4921 gaaatcgcca ccaccagcag cattttgcgt tcagcgtagt gctgtaaagc cagcacgaac
     4981 aggtctatct gattaacata attattaata ttggcctgat accacggcaa atcgcccttg
     5041 ctgagccgat tattcatctc cagccagttg gcattcagat gcgcatagcg agttttta
//
LOCUS       DAZSNM010000243         5024 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZSNM010000243 DAZSNM010000000
VERSION     DAZSNM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.243"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="ACHW42_003747"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="ACHW42_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFR8945370.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="ACHW42_003748"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="ACHW42_003748"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFR8945371.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="ACHW42_003749"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="ACHW42_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFR8945372.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="ACHW42_003750"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="ACHW42_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR8945373.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5024
                     /gene="yidZ"
                     /locus_tag="ACHW42_003751"
     CDS             4378..>5024
                     /gene="yidZ"
                     /locus_tag="ACHW42_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR8945374.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1144 a         1208 c         1256 g         1416 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cctg
//
LOCUS       DAZSNM010000244         4973 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZSNM010000244 DAZSNM010000000
VERSION     DAZSNM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.244"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHW42_003752"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHW42_003752"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFR8945375.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="ACHW42_003753"
     CDS             1020..1703
                     /locus_tag="ACHW42_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFR8945376.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="ACHW42_003754"
     CDS             1700..2161
                     /locus_tag="ACHW42_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFR8945377.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="ACHW42_003755"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="ACHW42_003755"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR8945378.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="ACHW42_003756"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="ACHW42_003756"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFR8945379.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHW42_003757"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHW42_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFR8945380.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHW42_003758"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHW42_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFR8945381.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(4903..>4973)
                     /locus_tag="ACHW42_003759"
     CDS             complement(4903..>4973)
                     /locus_tag="ACHW42_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945382.1"
                     /translation="NYSEYSEYTSVFFLSLMIFIKS"
BASE COUNT         1176 a         1305 c         1239 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAZSNM010000245         4969 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZSNM010000245 DAZSNM010000000
VERSION     DAZSNM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.245"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            241..1185
                     /gene="leuO"
                     /locus_tag="ACHW42_003760"
     CDS             241..1185
                     /gene="leuO"
                     /locus_tag="ACHW42_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR8945383.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1340..1384)
                     /locus_tag="ACHW42_003761"
     CDS             complement(1340..1384)
                     /locus_tag="ACHW42_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945384.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1503..3227
                     /gene="ilvI"
                     /locus_tag="ACHW42_003762"
     CDS             1503..3227
                     /gene="ilvI"
                     /locus_tag="ACHW42_003762"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR8945385.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3230..3721
                     /gene="ilvN"
                     /locus_tag="ACHW42_003763"
     CDS             3230..3721
                     /gene="ilvN"
                     /locus_tag="ACHW42_003763"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR8945386.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3733..3819
                     /locus_tag="ACHW42_003764"
     CDS             3733..3819
                     /locus_tag="ACHW42_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR8945387.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3901..4905
                     /gene="cra"
                     /locus_tag="ACHW42_003765"
     CDS             3901..4905
                     /gene="cra"
                     /locus_tag="ACHW42_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR8945388.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1207 a         1134 c         1309 g         1319 t
ORIGIN      
        1 tcgcggtagt tatgattaga ttgttttcgc aacaaaacat tatggattat tatgctgtgg
       61 taaatgactc attccacggc aatggattct gtttttatca gaacccgtat ctttatgttt
      121 tccgaatttt actcattttg ctttttctta ttttatatgc atgataaatc atattcttca
      181 ggattatttc tctgcattcc aataagggaa agggagttaa gtgtgacagt ggagttaagt
      241 atgccagagg tacaaacaga tcattcagag acggcggagt taagcaagcc acagctacgc
      301 atggtcgatc tcaacttatt aaccgttttc gatgccgtga tgcaggagca aaacattacc
      361 cgtgccgctc atgttctggg tatgtcgcaa cctgcggtca gtaacgctgt tgcacgcctg
      421 aaggtgatgt ttaatgacga gctttttgtt cgttatggcc gtggtattca accgactgct
      481 cgcgcatttc aactttttgg ttcagttcgc caggcattgc aactagtaca aaatgaattg
      541 cctggttcag gttttgaacc cgcgagcagt gaacgtgtat ttcatctttg tgtttgcagc
      601 ccgttagaca gcattctgac ctcgcagatt tataatcaca ttgagcagat tgcgccaaat
      661 atacatgtta tgttcaagtc ttcattaaat cagaacactg aacatcagct gcgttatcag
      721 gaaacggagt ttgtgattag ttatgaagac ttccatcgtc ctgaatttac cagcgtgcca
      781 ttatttaaag atgaaatggt gctggtagcc agcaaaaatc atccaacaat taaaggcccg
      841 ttactgaaac atgatgttta taacgaacaa catgcggcgg tttcgctcga tcgtttcgcg
      901 tcatttagtc aaccttggta tgacacggta gataagcaag ccagtatcgc gtatcagggc
      961 atggcaatga tgagcgtact tagcgtggtg tcgcaaacgc atttggtcgc tattgcgccg
     1021 cgttggctgg ctgaagagtt cgctgaatcc ttagaattac aggtattacc gctgccgtta
     1081 aaacaaaaca gcagaacctg ttatctctct tggcatgaag ctgccgggcg cgataaaggc
     1141 catcagtgga tggaagaaca attagtctca atttgcaaac gctaactgaa tgcagaatag
     1201 gccagacatg aatgtctggt ttattctgca ttttttattg aatgtagaat tttattctga
     1261 atgtgtggac tctctatttt aggattaatt aaaaaaatag agaaattgct gtaagttgtg
     1321 ggattcagcc gatttattat caatttaatc cttcgtaatg gaggatttta tcgtttcttt
     1381 tcacctttcc tcctgtttat tcttattacc ctgcgtttat gtctctggct gccaattgct
     1441 taagcaagat cggacggtta atgtgtttta cacatttttt ccgtcaaaca gtgaggcagg
     1501 ccatggagat gttgtctgga gccgagatgg tcgtccgatc gcttatcgat cagggcgtta
     1561 aacaagtatt cggttatccc ggaggcgcag tccttgatat ttacgatgca ttgcataccg
     1621 tgggtggtat tgatcatgta ttagttcgtc atgagcaggc ggcggtgcat atggccgatg
     1681 gcctggcgcg tgcgaccggg gaagtcggcg tcgtgctggt aacgtcgggt ccaagggcga
     1741 ccaatgcgat taccggcatc gccaccgctt atatggattc cattccatta gttgtccttt
     1801 ccgggcaggt agcgacctcg ttgataggtt acgatgcctt tcaggagtgc gacatggtgg
     1861 ggatttcgcg accggtggtt aaacacagtt ttctggttaa gcaaacggaa gacattccgc
     1921 aggtgctgaa aaaggctttc tggctggcgg caagtggtcg cccaggacca gtggtcgttg
     1981 atttaccgaa agatattctt aatccggcga acaaattacc ctatgtctgc cctgagtcgg
     2041 tcagtatgcg ttcttacaat cccactacta ccggacataa agggcaaatt aagcgtgctc
     2101 tgcaaacgct ggtagcggca aaaaaaccgg ttgtctacgt aggcggtggg gcaatcatgg
     2161 cgggctgcca tcagcagttg aaagaaacgg tggaggcgtt gaatctgccc gttgtttcct
     2221 cattgatggg aatgggggcg tttccggcaa cgcatcgtca ggcactgggc atgctgggaa
     2281 tgcacggtac ctacgaagcc aatatgacga tgcataacgc ggatgtgatt ttcgccgtcg
     2341 gggtacgatt tgatgaccga acgacgaaca atctggcaaa gtactgccca aatgccactg
     2401 ttctgcatat cgatattgat cctacttcca tttctaaaac cgtgactgcg gatatcccga
     2461 ttgtggggga tgctcgccag gtcctcgaac aaatgcttga actcctgtcg caagaatccg
     2521 cccatcaacc gctggatgag atccgcgact ggtggcagca gattgaacag tggcgcgctc
     2581 ttcagtgcct gaaatatgac actcacagtg aaaagattaa accgcaggcg gtgatcgaga
     2641 ctctttggcg gttgactaat ggggacgctt acgtgacgtc cgatgtcggg cagcaccaga
     2701 tgtttgctgc gctttattat ccattcgaca aaccgcgtcg ctggatcaat tccggcggtc
     2761 tcggcacgat gggttttggt ttacctgcgg cactgggcgt caaaatggcg ttgccagaag
     2821 aaaccgtggt ttgcgtcact ggcgacggca gtattcagat gaacatccag gaactgtcta
     2881 ccgcgttgca atacgagttg cccgtactgg tggtgaatct caataaccgc tatctgggga
     2941 tggtgaaaca gtggcaggac atgatctatt ccggccgtca ttcacaatct tatatgcaat
     3001 cactacccga tttcgtccgt ctggcggaag cctatggtca cgtcgggatc cagatctctc
     3061 atccgcatga gctggaaagc aaacttagcg aggcgctgga acaggtgcgc aataatcgcc
     3121 tggtgtttgt tgatgttacc gtcgatggca gtgagcacgt ctacccgatg cagattcgcg
     3181 ggggcggaat ggatgaaatg tggttaagca aaacggagag aacctgatta tgcgccggat
     3241 attatcagtc ttactcgaaa atgaatcagg cgcgttatcc cgcgtgattg gccttttttc
     3301 ccagcgaggc tacaacattg aaagcctgac cgttgcgcca accgacgatc cgacattatc
     3361 gcgtatgacc atccagaccg tgggcgatga aaaagtactt gagcagatcg aaaagcaatt
     3421 acacaagctg gtcgatgtct tgcgcgtgag tgagttgggg cagggcgcgc atgttgagcg
     3481 ggaaatcatg ctggtgaaaa ttcaggccag cggttacggg cgtgacgaag tgaaacgtaa
     3541 tacggaaata ttccgtgggc aaattatcga tgtcaccccc tcgctttata ccgttcaatt
     3601 agcaggcacc agcgataagc ttgatgcatt tttagcatcg attcgcgatg tggcgaaaat
     3661 tgtggaagtt gctcgctctg gtgtggtcgg actttcgcgc ggcgataaaa taatgcgttg
     3721 agaatgatct cagtgcgcaa tttgcagccc aacatgtcac gttgggcttt ttttgcgaaa
     3781 tcagtgggaa cctggaataa aagcagttgc cgcagttaat tttctgcgct tagatgttaa
     3841 tgaatttaac ccataccagt acaatggcta tggtttttac attttacgca aggggcaatt
     3901 gtgaaactgg atgaaatcgc tcggctggcg ggagtgtcgc ggaccactgc aagctatgtt
     3961 attaacggca aagcgaagca ataccgtgtg agcgacaaaa ccgttgaaaa agtcatggct
     4021 gtggtgcgtg agcacaatta ccacccgaac gccgtggcag ctgggcttcg tgctggacgc
     4081 acacgttcta ttggtcttgt gatccccgat ctggagaaca ccagctatac ccgcatcgct
     4141 aactatcttg aacgccaggc gcggcaacgg ggttatcaac tgctgattgc ctgctcagaa
     4201 gatcagccag acaacgaaat gcgctgcatt gagcaccttt tacagcgtca ggttgatgcc
     4261 attattgttt cgacgtcgtt gccacctgag catccttttt atcaacgctg ggctaacgac
     4321 ccgttcccga ttgtcgcgct ggaccgcgcc ctcgatcgtg aacatttcac cagcgtggtt
     4381 ggtgccgatc aggatgatgc cgaaatgctg gcggaagagt tacgtaagtt tcccgccgag
     4441 acggtgcttt atcttggcgc gctgccggag ctttctgtca gcttcctgcg tgaacaaggt
     4501 ttccgtactg cttggaaaga tgatccgcgc gaagtgcatt tcctgtatgc caacagctat
     4561 gagcgggagg cggctgccca gttattcgaa aaatggctgg aaacgcatcc gatgccgcag
     4621 gcgctgttca caacgtcgtt tgcgttgttg caaggagtga tggatgtcac gctgcgtcgc
     4681 gacggcaaac tgccttctga cctggcaatt gccacctttg gcgataacga actgctcgac
     4741 ttcttacagt gtccggtgct ggcagtggct caacgtcacc gcgatgtcgc agagcgtgtg
     4801 ctggagattg tcctggcaag cctggacgaa ccgcgtaagc caaaacctgg tttaacgcgc
     4861 attaaacgta atctctatcg ccgcggcgtg ctcagccgta gctaatccgc gaacaaaaat
     4921 acgcgccagg tgaatttccc tctggcgcgt agagtacggg actggacat
//
LOCUS       DAZSNM010000246         4926 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZSNM010000246 DAZSNM010000000
VERSION     DAZSNM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.246"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..971
                     /locus_tag="ACHW42_003766"
                     /pseudo
     CDS             1..971
                     /locus_tag="ACHW42_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
     gene            complement(1125..2774)
                     /locus_tag="ACHW42_003767"
     CDS             complement(1125..2774)
                     /locus_tag="ACHW42_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR8945389.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2925..4274)
                     /gene="ghxP"
                     /locus_tag="ACHW42_003768"
                     /gene_synonym="yjcD"
     CDS             complement(2925..4274)
                     /gene="ghxP"
                     /locus_tag="ACHW42_003768"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR8945390.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
BASE COUNT         1405 a         1294 c         1155 g         1072 t
ORIGIN      
        1 acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt atattttata
       61 aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa tggtggttac
      121 gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt atccaattcg
      181 aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat gtataaaacg
      241 aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct cgatgactca
      301 aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc tatgcatgtt
      361 caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct tgactattca
      421 aatttttata tgcgtacatg gctgaggtaa atctttataa agtaatagcg ccatatgtta
      481 atttatttaa agccgacctt agtttctcta aactcgattt aattaacttt gaacatgctg
      541 atctgtctcg cgtcaatctg aacaaagcaa tcctccagaa tataaactta attgatagca
      601 aactcttttg tacgtggctg acaaatacat tcctcgaaat ggttatatgt accggctcta
      661 atatggctaa tgttaatttt aataatgcca atttaagcaa ctgccatttc aactgttcta
      721 ttttaacaaa agcctgtatg tttaataccc gtctctatcg ggttaatttc gatgaggcta
      781 gcgtccaggg aatgggcatt tccattctcc gtggggagga aaatatcccc attgatagtg
      841 ataccctggt aacactacag aaattctttg aagaagattg tacctctcat actggcatgt
      901 cacaaactga ggataatatt aatgcagtcg ctatgaagat tactgcagat attatgcaac
      961 acgcagattg aggttaagtc gaagaacagc caatgaattg actttacaag agagataact
     1021 tatttgtttt agaatcatga atgttcgccc gagctaacct gttaaaggtg caaaagattg
     1081 cacctttttc agattacctc gggcctggtt acgtcgcttt cggcttactg attttcctca
     1141 attagcaacg cttcaagcaa atcgagatcg tgcagtaatt tttgcagcgt ttcgttgctg
     1201 atctcccgcg tggcgcgcag gtggtacagt tcagcacgtt cagaacgcaa tgccgccaga
     1261 cggaagcgac gctcaaggtt ctcttcctgc acggaacttt caacgtcgtt acgtccatcg
     1321 gcgcgacgac gcaggttacc aatgacgcga gaactgacct ccgtaagcaa ctggttatcg
     1381 atattctctt cggtatccgc cgccagacgc tcctccattt tctggatcgc cacaatcgcc
     1441 acttctgccg ttgccgctcg cgcaatacgt tcttctttca attgttgcga atggtccgcg
     1501 acttcaatgt gttgtagcag aataggcaac atcaccacgc cgacaaacag cgagaagaga
     1561 atgacgccag ccgccaggaa caccagctca tagcgcgccg ggaagacgtt accatccggc
     1621 aagagcagcg ggattgagag cacaccggcc agagtgatcg ccccacgcac cccagcgaaa
     1681 gacgcgatca ggatttctcg tgtggtccac gaaccaaact ccatcggctt cttcttcagg
     1741 aagcggttgc tgaacttttt catcgtccac agccagccga aacggaccag catcagcgcc
     1801 gcatatatca gaataatatc ggtaaacagc atccagattt cgacgttagg gtcgatttct
     1861 gccgccatca gcgacgtctc cagaataccc ggcagttgca gacctaacag caggaacacc
     1921 atgccgttaa agacaaattc cagcatcgcc caggtgctgt ttgcgcgcag gcgcattgcc
     1981 agtggcgcac ggcgcatcac accggagcgg gtgatggtca tcccggcggc aaccgcagcg
     2041 aggatgcccg aaacgccaat atgttcggca atcagataag aagcgaatgg cagcaagaac
     2101 agcagaacga tctgcgtcgc gggttcatca ccgccccagc ggctgaggaa tcgcagcgag
     2161 cgaccataca gccagctcac cacaaaaccg gcaagaatac cgccaatggc tactttcata
     2221 aattcgaccg tcgcaccgcc gacagtgaag atcatcgtcc ccatcgccac tgccacggca
     2281 aacttcaacg acaccaggcc ggaagcgtca ttcatcaacg cttcgccctg caaaatgccc
     2341 atgatttttt tcgggatgcg cccttcgcca acaatcccgg agagcgccac agcatcggtc
     2401 ggagaaagta ccgccgccag cgcaaaggcg gggatcagcg gaatgcccgg caccacccag
     2461 taaataagga agccaatgcc gaccacggtg accaccacca gcgccagcgc gaggccgaaa
     2521 atctctcgac catgttcgag aaattcacgg gtcggcgttt tccagccatc agcgaacagc
     2581 aacggcggga taaacaagac taaaaagagt tcaggatcaa actccacatg caaaccaaac
     2641 gtcggccacg ccagtagcgc accgatggcg atttgcataa gcggaagcgg gatctgaaag
     2701 ggcatgacac gagtgaccac cccggacagc gagaccacga gggtcattat cagtatggtg
     2761 aagaagattt ccatgcgttc cctgtttgcg atgtttttta tgatctgaga aagacgttat
     2821 gcctgaattc tcattttttc agaatatcgc aaaagctcac acaatgtgcg gagaaaataa
     2881 aaacgggcag ccattgccac ccgttttcag aatccgatga aagattagat agcccagcca
     2941 cccgcataga aggtcaccag cgccacggcg ataacgaccg taccgatgtt caacttgcgc
     3001 cattcaccgg aaaccagacg accaatcacc agagtcgcga agccgatcat gatgcctgtt
     3061 acgatgttac aggtcagcac gatgaatacc gccgtaacca gacccgccat cgcatcaaca
     3121 aaatcagcaa agtcgatttt cgccacgttg ctcagcatca gcaggccaac gtacatcagc
     3181 gccggagccg ttgcataccc cggaacgagg taagaaagcg gagagaggaa cagaatcagc
     3241 aggaacagca cgccaacggt gatagccgtc aacccggttt taccgcccgc cgccgtaccc
     3301 gccgcagact cgatgtatac cgctgccgga gctgcaccca ccaggccaga gaaaacgctg
     3361 ctcatggagt cagtggtcag tgctttcccg ccgtcgatga tctgcccatc tttatccagc
     3421 aggttcgcct ggccggcgac agcgcggata gttccggtgg catcaaatac tgccgtcatc
     3481 accagcgcca gaacgcttgg caggactaca ggattcagcg cgcccataat gtccaggctg
     3541 ccaatcagtg aattgccgtt ttcatcgctc aatgaaggca tggcgaaaac gccggagaaa
     3601 tggacgttag gatcgaagat caaaccgaca attgagatac cgataatggt cagcagaatg
     3661 ccaccaggga ctttcagttt ttccaagccg atgatcaccg ccagacctgc cagtgacata
     3721 atcaccggga aggtcgcgaa atcacccagc gcaacgggca gaccatcaag cgggttttta
     3781 atcaccagac cgacaccatt agcggcaatg agcagcagga acagaccgat accgatcccc
     3841 gtgccgtgcg ccacaccgtg cggcaagttg cgcaaaatcc agctacggat acccgtggca
     3901 gaaattaccg taaacagcac acccatcagg aacacggcac ccagcgcgac aggtacgcta
     3961 atatgttgcc ccagcaccag gctgaatgcg gtaaacgccg tcagggagat ggcgcaacca
     4021 atcgccaacg gcagattagc ccacagaccc atcacgatag aaccgagtcc ggcaaccaga
     4081 caggttgcaa cgaaaactgc cgcaggcggg aagcctgctt tacccaacat acctggaacg
     4141 acgatgaccg agtagaccat cgccagaaac gttgttaacc cggcaaccac ttcctgacgg
     4201 acagtgcttc cacgttgtga aattttaaac caggcgtcga gtgaaccgcc ggtacgcgct
     4261 gatggcgtag acataagaaa acatcccctg agagtttaat tttcgtcaag atgcgtggcg
     4321 gacaatcccc tgccagcgaa acgttatctt cctgctccag gtttgcggca atttggtgcg
     4381 tcgcaaaaaa gcaaacgttt agctccgcgc atacaaaacg ggtggtaaaa aaaagcaaac
     4441 gattatccgg cgatcatagc ggctttttca accgaagttt gctgcttttt tctcttttat
     4501 tgtcttgacg atgaaaaacc aggcaattga tgcgcaaacg ttatcgtcga tgtgcaagat
     4561 tgcagcgtta tcgagccgta taccgttctg gattgcgata aaaaatcgtc tgagatgaca
     4621 aaaatattgc ggctaaaaag tatactttta cactggcgaa gaaacgtcgg tgacatacaa
     4681 ttaaagcatc aacaccaacc ggaacctcca ccacgtgctc gaatgaggtg tgttgacgtc
     4741 gggggaaacc ctcctgtgtt ccagcgggat agagagaaag acaaagaccg gaaaacaaac
     4801 taaagcgccc ttgtggcgct ttagttttgt gtttgcggat aattccaaaa gagctcgatc
     4861 agtcccctcg ccccttcggg gagagggtta gggtgagggg aacaggcagc accagtgcaa
     4921 atattt
//
LOCUS       DAZSNM010000247         4883 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZSNM010000247 DAZSNM010000000
VERSION     DAZSNM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.247"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(96..472)
                     /gene="rnpB"
                     /locus_tag="ACHW42_003769"
     ncRNA           complement(96..472)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHW42_003769"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(505..1650)
                     /gene="garK"
                     /locus_tag="ACHW42_003770"
     CDS             complement(505..1650)
                     /gene="garK"
                     /locus_tag="ACHW42_003770"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR8945391.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1747..2637)
                     /gene="garR"
                     /locus_tag="ACHW42_003771"
     CDS             complement(1747..2637)
                     /gene="garR"
                     /locus_tag="ACHW42_003771"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR8945392.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2667..3437)
                     /gene="garL"
                     /locus_tag="ACHW42_003772"
                     /pseudo
     CDS             complement(2667..3437)
                     /gene="garL"
                     /locus_tag="ACHW42_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3453..4787)
                     /gene="garP"
                     /locus_tag="ACHW42_003773"
     CDS             complement(3453..4787)
                     /gene="garP"
                     /locus_tag="ACHW42_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR8945393.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1166 a         1400 c         1173 g         1144 t
ORIGIN      
        1 agccgctttc ttttgggtat agcgtcgtgg acagtcattc atctttctgc ccctccaaaa
       61 gcaaaaaccc gccgaagcgg gtttttacgt aaaacaggtg aaactgaccg ataagccggg
      121 ttctgtcgtg gacagtcatt catctaggcc agcaatcgct cactggctca agcagcctac
      181 ccgggttcag tacgggccgt accttatgaa cccctatttg gccttgctcc gggtggagtt
      241 taccgtgcca cggactgtta ccagccgcgc ggtgcgctct taccgcaccc tttcaccctt
      301 acctgatccc gcttgcacgg gccatcggcg gtttgctctc tgttgcactg gtcgtgggtt
      361 tcccccccag gcgttacctg gcaccctgcc ctatggagcc cggactttcc tcccctccac
      421 ccgtctcccc cgaagaggac gacgacgaag cggcgactgt ctggtcagct tcggcgcgca
      481 gtatagaggg tttgcgcgcc cttgtcaccc cgtgttgcgc attccaatcg ccagtgtcgc
      541 ggcaatatta cgtgaagcac ggcagatatt gtcataagcc ccgcggaatg cttcgtccaa
      601 cgtacctatg ctggtcaata cgctgaagac cgcatcaatg ccatgctgat gtacaacgcc
      661 aacatcatcg gtcaggctac ccgcaatgcc aatcaccggt ttatggtact tcttcgccac
      721 gtttgcgacg ccaatcggta ccttgccgtg aatactctgg ctgtcaatac gcccttcacc
      781 ggtgatcacc agcgtacaat cgtgaatatg ttcctccaga ttcagcgccg tagtgacgat
      841 ttcaatacca cttttcagtt ccgcaccaag aaacgccatc agcgccgcac ccataccgcc
      901 cgcagctcct gcgccgggga catcttttac atcaacatgc agcgcttttt taatgacatc
      961 ggcatagtga gagaggttat tgtccagctc aacaatcatc gcttcactgg ctcccttttg
     1021 tgggccaaag atgcgcgatg cgccgttatc gcccaccagc ggattggtga catcacaagc
     1081 gacgcgaatg acgcaatctt ttaagcgcgg atcgaggccg gaaatatcaa tatcattcag
     1141 agtattaaga ctaccgccgc caaaaccaat ttcattgccg ttggcgtcgc ataatttcgc
     1201 ccccagcgcc tgtaccatgc ctgcgccgcc atcatttgta gcgctaccgc caatgccgat
     1261 aataatgttt gtcgcgccgc tctccagcgc ctgcaggatt aactcgcctg tgccgcgtga
     1321 agtggtcacg agtggatcgc gtttttccgc aggtaccagc tccagcccac tggccgccgc
     1381 catttcaata aacgcggttt tgccatcgcc ggagatcccc caactggcat tcactttctc
     1441 gcccagcggc cctgtaaccc aggcgtgacg ttcagaaccc tgggtggctg caatcatcgc
     1501 ttccaccgtt ccttcgccac cgtcggcaac cggaatagaa acgtactgtg catcaggaaa
     1561 aatttcccga aatccttttt ctatcgcctg cgcaacctcg ctggcagata aactttcttt
     1621 ataagagtct ggggcgatta cgattttcat acctatgcct gttaccacat gacgccggag
     1681 ggcgtttctc ttattcggcc tggattccag gcccggattg caatacgcca tccgggcacg
     1741 acgtcattaa cgagtaactt cgactttcgc cagtttttcg tagtagcaca ccagggcgct
     1801 atgatccgcc gttcctaaac catctgctcg cagtgcctgc atcatctcca taaccgcagc
     1861 tgtgagcggc agttgcgcgc cgacgccgtg ggaagtatcc agcgcatttg ccagatcctt
     1921 aatatgcaga tcaatacgga agcccggctt gaagttgcgg tccatcacca tcggcgcttt
     1981 ggcatccagc acggtactgc ccgccagtcc accgcgaatt gcctgataca ccaggtccgg
     2041 gttaacgccc gctttagttg ccagcgttaa cgcttctgac atcgcggcaa tattcagcgc
     2101 cacaatgacc tgattcgcca gtttggtgac gttacctgca ccaattgccc cggtatgcac
     2161 cacggaaccc gccattgctt tcatcaaatc atagtatttg tcgaaaatag ccttgtcgcc
     2221 gcccaccatc actgacagcg taccgtcgat ggctttcggt tcaccgccgc tcaccggagc
     2281 atccagcata tcaatgcctt tcgctttcag cgcttcgctg atttcacggc ttgccagcgg
     2341 tgcgatagaa ctcatatcga tcaataccgt acctggcttc gcgccttcaa taatgccatt
     2401 ctcacccagc gccacctctt tcacatgagg ggagtttggc agcatggtta tgatgacgtc
     2461 gcactgttca gcgatcgctt tagccgtaga ggctgtttct gcacctgcag caatcacgtc
     2521 agcaatagct tctgggttac ggtcagcaac caccagcgag taacctgctt tcagaaggtt
     2581 tttactcatt ggtttaccca taatccccag gccaataaaa ccaactttca tagtcatatc
     2641 aatcatctct cttgttgcgg tggtggttat tttttaaagg tatcagccag tttatgagtg
     2701 gcagagcgga agacgccgag atcgctgccg acagccacaa acgtcgcgcc ccattccaga
     2761 taacgacgcg catcggcttc gaccggcgcg aggataccgc tgggtttgcc gtgcgcgctg
     2821 gcacggttaa aaatgtgctg aattgctttt tgtacatccg ggtgtgatgc attgccgaga
     2881 tggcctaatg ccgcggccag atcgctgggg ccgacgaaga tgccgtctac gccttcggta
     2941 gcggcaatgg catcgacgtt atctacgccc tgctgacttt ctatctggac cagaatagtg
     3001 atgttcttgt tcgactgagc gaaataatcc gccacggtgc caaacatatt ggcgcggtga
     3061 gaaacggaga cgccgcgaat gccttccggt gggtaacggg ttgatgccac cgcctgctct
     3121 gcttcctctt ttgtttctac aaaaggaatc aggaagttat agaaaccgat atccagaaga
     3181 cgcttaataa tttccggctc gttggtcggc actcacacta ctggcgcgct ggcgctgcct
     3241 ttcaaggcca ttaactgcgg aataaacgtg gagatatcgt ttggcgcatg ttcgccatcc
     3301 agcaccagcc agtcaaaccc agccaaacca agaacttcag tgctaatcgg gttagagagt
     3361 gctgaccagc aaccaatttg tacctgtttc gcagccagtg cggctttgaa tttattcggg
     3421 aaaacatcgt tattcatcgc ttataccttt gcttatttct gcaattccat acgtttaatg
     3481 tcgccaacta cgaagaggta gcagaccatc gccatcagcg ctgaacatcc cacgaaaacc
     3541 agtgctgcat tgaaggagtg cagttcactt accaggtagc caatcaccag tggagtgaca
     3601 atggaggcaa cattgccaaa gacgttaaag acgccgccac agaggccaac aatctctttc
     3661 ggcgcggtgt cagaaatcac cggccagccc agagcaccaa atcctttgcc aaaaaaagcc
     3721 agcgccatca gcatgaccac cagcgtggtg ttgttggtgt agttacataa gatgatggtg
     3781 gaagccagca acattcccag cacaatcggt agcttacgtg ccagggtcag ggataaaccg
     3841 cgtttgatca gataatccga gaagacacct cccagcacgc cgcccgcaaa accacacagt
     3901 gccggaatcg aggcgaccag acccactttc agaatcgaca tgcctttttc ctgcaccaga
     3961 taaatcggga accaggtgag gaagaaccag gtgatggtgt tgataaaata ttgtccgaaa
     4021 aatacgccca acatcatgcg gttagagagc aattgcttga tgtaatgcag tttgggaccg
     4081 cttgctgccg cgctgcccgg ctttttgtgg tccatatcga ccaccgcgcc attttcagag
     4141 ataaacttca gctcttcctc agacatacgt gggtgatctg tcgggttatg aatcaacttg
     4201 atccacagcg ccgtcagcac aaaacaaatc acccccataa cggtaaagac gtgctcccat
     4261 ccccaggcga aagtcagcca gccaagcaga ggcgaaaaga gcgccagcga gaaatattgt
     4321 gccgagttaa agatggcgga ggcagtacca cgttctttcg tcgggaacca ggcggcgaca
     4381 atacgggcgt tcgccgggaa ggatggagct tccgagaatc ccagcataaa gcgcataaag
     4441 aacatggaga tccctgccca agccagcggg aacatatcaa caaagccttg caggaaggtg
     4501 aacagcgacc agaaaaagag gctgtaggtg taaacttttt tcgagccaaa cttatcaagc
     4561 aaccagccgc cggggatttg catcagcaag taggcccagc caaaagcgga gaagatgtaa
     4621 cccatcgaaa ccgcacttaa ctgcaactct tttgccactt cggtaccagc aatagacagc
     4681 gttgcacgat cggcgtagtt aacggcggta acaataaaaa taatcagtaa tattaaatag
     4741 cgggtatgca cacctttcct tttttcgtca acggtgtcca gaatcatttt atttacctcg
     4801 ggtacttatg ctgattttta ttattatggg gaaggtgtta tttataagtt tcatttatgc
     4861 cgtaacgaca atgaactcgg gaa
//
LOCUS       DAZSNM010000248         4879 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZSNM010000248 DAZSNM010000000
VERSION     DAZSNM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.248"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..591)
                     /gene="mxiC"
                     /locus_tag="ACHW42_003774"
     CDS             complement(<1..591)
                     /gene="mxiC"
                     /locus_tag="ACHW42_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR8945394.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTD"
     gene            complement(610..2310)
                     /gene="mxiD"
                     /locus_tag="ACHW42_003775"
     CDS             complement(610..2310)
                     /gene="mxiD"
                     /locus_tag="ACHW42_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFR8945395.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            complement(2297..2929)
                     /gene="mxiE"
                     /locus_tag="ACHW42_003776"
     CDS             complement(2297..2929)
                     /gene="mxiE"
                     /locus_tag="ACHW42_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HFR8945396.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            complement(3059..3487)
                     /gene="mxiM"
                     /locus_tag="ACHW42_003777"
     CDS             complement(3059..3487)
                     /gene="mxiM"
                     /locus_tag="ACHW42_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HFR8945397.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            complement(3471..3878)
                     /gene="mxiM"
                     /locus_tag="ACHW42_003778"
     CDS             complement(3471..3878)
                     /gene="mxiM"
                     /locus_tag="ACHW42_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HFR8945398.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            complement(3871..4566)
                     /gene="mxiN"
                     /locus_tag="ACHW42_003779"
     CDS             complement(3871..4566)
                     /gene="mxiN"
                     /locus_tag="ACHW42_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HFR8945399.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            complement(4508..>4879)
                     /locus_tag="ACHW42_003780"
     CDS             complement(4508..>4879)
                     /locus_tag="ACHW42_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mxiK"
                     /protein_id="HFR8945400.1"
                     /translation="LNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVI
                     SYYGGKISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLS
                     RLNEGMQYAKRHFTGIQTSCL"
BASE COUNT         1556 a          952 c          647 g         1724 t
ORIGIN      
        1 atctgttgtt gatatgttcc ccatgataaa accacgataa catgcacgca aaagctgtgg
       61 ttcaagtttc attttgttgc caaataattt agcctgtata gctgaattta ttccagcatt
      121 aacacttttt tcactacctg atattatctc attaataatt ttagttaata attcgacttg
      181 ttcagcagta agatcttttt cattcaaaag ttcccttaat gctaagactt gatcacttgg
      241 atctttaaaa tatctcttta gtctgtctat aaaatcccga tctagaggaa ggttatcttt
      301 taacgttctc tttaaatcaa aaatcttgtg attaatttca tcttcttccc catctaaaat
      361 acgttcctga ctatcactat ttgttccttt cagtttctca aggtctcttc tgtttataaa
      421 tgacgaaaga gcggaagcca tctcatcaga tgagtcaata tacttgctat tagccaagcc
      481 agaatcaagc tctgcatcag cagtctcatc tccatcatct gaatttgtca ttgcattcaa
      541 cttatcaatg aatgcagagc taaaaactcc tgtattttta acatcaagca tacatcacct
      601 actttatagt tagtaattta agtatgaaac caatgatttt tcgtcttcca gcaaagtagt
      661 ttcagatggg attatttgtg tagtcttttg tatttctctt tcagatatga ggcttttata
      721 ttctgcagta ttataataac ttgactcctt tatctcacgg ggttgaatca gaaaaaccct
      781 tacaatatta ctgatattac ttgttttata cttgaacaca ttaccgatta caggaataga
      841 ggagagaaaa ggaatagata ttatttcgtt agagttcgtt tcatgcgtat aaccacctat
      901 caacaaactt ttgccttgtg gaactctcgc tattgtactg attttggtcc ttccaacttc
      961 tggaaggaca gatgtatttt cattattata attgtaattt gactgactat ttcctgttcc
     1021 atcctctatc gttaaagaca tctcaatttg tccccgagat gaaaagcgag gtataacatt
     1081 tatcaatgtt ccatacgtaa catgctccaa cgaagaattg cgctcaccaa ctaatgacac
     1141 ataaaaagtc ctgttattat caaatatagc cggaatattc tcttgtgtca aaatcaccgg
     1201 ccttgaaact acatttgctt tctttttctg atttaatgcc ataactgaag caataaactt
     1261 attgccgtcc aaagtgctta tcgatgctga tgagctcata ttaaatgacg caccaaacga
     1321 atcaccaaat gaagccgttc cttgccaatt aacaccaagg ttatttaatt cagatttatc
     1381 aatatcgatt atccataatg ataattctat atgtcgtttg gctatatcta actgagtgat
     1441 aatatcgcga ataatctgta tttgctgatc attacctttg actaatatag aattggtttc
     1501 tggatatgca ataagtgata catcctctaa tatagaacta ttggtaactg aactaaatga
     1561 aaagtcatta ctatcctcag atactttctg tgtaatatta aagggtggca tcggatcatt
     1621 ttgggcttgt ctgttactca gagccttacc attatttaat aaacgttcta caacagtagc
     1681 cacccctggg atgacaatat cctcccctct catattataa gttctgtcac taacaaatgt
     1741 attttttaat tttataaccc caaaattaac tttatctgtc ccaatagaag aaacctgctt
     1801 atcaagcaaa gttgctgtat tagctaccag ttcaaccaat gccggaggac cagatatata
     1861 gaatgtttta tcactaatgt ttcctcttat aggatatctg tgatcataaa ggttagcatc
     1921 cttcagatac tgaataagat aattaagtga tatattctcc agcaaaataa ctttgcttat
     1981 taattctcca gagtcatata tgtataatgc atttccatct ttataccata tgagtcccac
     2041 aagtaatgtc agtttttcaa gcatttcttc tggattagat aaatcaaact ccccagatat
     2101 tctctttttt gcagcttgtt tgctaacaac aataggataa tttaataacg cagaaaaacg
     2161 ttcaaaaaaa gatcctaccg tatcactttg agcaacatat ttcgctatgt cattttgttc
     2221 taataaatga gagtcaatat tattagcatt aactatcaat ggtaacaata caatcaagag
     2281 agtcaaagat ttaatattaa attttttcat ttattttttt cactaaaaaa gtaatattcg
     2341 ataattctct tgcagaaaag cctagtcgtg tttttatctc atttgaaaaa tgggatgttg
     2401 atgcataccc attattcatg gcagctgaag tgattgtttg attgtgcaaa aatacatcta
     2461 ataaaccatt tactaaccgc caagtgttca gttgttcttt gacttttgcc cctaaagctt
     2521 ttctacataa agatctaaaa taagcctccg acacaccata atgctctgtc agactcttta
     2581 ttcttacctc cttttctttc tcaatagttc tcagaagata aagaaccaaa tgatatgact
     2641 cagtcattcg tatcatagcc agtgcattac ttacattctg gtcagatgaa aagatgaatc
     2701 ttaacattaa cttatcaagc atcgattcgg tattacaact atcccgaaga tcattctcat
     2761 gatagaaatg aatacatttt tcagcatggc tatgctctac tagagcacca ctgaaatagt
     2821 cccaaaaagc aagaaatttt gcacaactat taatgttaaa gcgataatga tagttcacat
     2881 tatcactgaa ttttatattt tgatttcgga caaaaaaaaa cccttccatg ctggagccag
     2941 ctcaattatg ttacgttctt tatcttttac aagctcaacg ttaactggtt catgtccact
     3001 agagtaaatg taaaacatat tacttgtatt tagaccttta tatttactca caatacaatt
     3061 atatccctcg aattaatttt aatttaactt ccccatcctt ctctataagg atggtatcat
     3121 ttatcttatt accttttaaa acaaccccgt ctttcagcat agaaaatagt ttatctatag
     3181 ctatcttttg tgcatccatg aagcacccat ctacttcctt aacattaaag ttgcccatga
     3241 atttacctgc attaaaacta taaaccgcct taccactaat acatttagag taaacattta
     3301 tacttttatt ggttgtatta aacgaccata ataaatcatt tggaatagaa aaataatcct
     3361 tacttacagg aacaatatgc cattcttttt cagaattaga tgatgacttt aaagcacacc
     3421 cactcagtgt taatagcaat atacttaaaa taaatatttt caacttatta ctaccatgtc
     3481 gaatcatctg cctctctccc tgcttcaaag acactaatta atgaatcgaa ggttgtagtc
     3541 ttaatataga aatcaatatt gttatatatg gacaatgatg cataaaatcc ctcatgcaaa
     3601 tcgacatatt gatttgcatc tatgagttta ttaagaagtg aacttttttg tgcagctgta
     3661 agataaggaa aacgtgaatt gaataattgt tcaatcttat tgtgcagttc atcattatct
     3721 atggaattta attcactctg tgagaaatat tgcattattt catagattat atcctcatca
     3781 aatgattgcg aagatgataa tctctcattt aaattaacaa cttcttcact atttaatctt
     3841 tgctgcagag cattgcttgc atttatttga ttaatcatta acaggattct cttttaacaa
     3901 gtcttctgca atttgtctca gtttcttatg tgctaactct ttgaaatatt ttttatcatt
     3961 ttttgttaat ttttccgcaa gttctatttt aacacctgat attacatcct gaggactaaa
     4021 ctccactact tgattaccac tacagaatat ataattattc ccttcagaat attttaaaat
     4081 aatcttgaca tctgagcgat atttatggag atcaagttct aactttttcc tcagtgctaa
     4141 attacacttg ggcaaaacca cttttaattc tgttactgta ttacgcaacg aactaagcca
     4201 ctgctccaat aaagtgctaa tgatcctctc atcatgtaaa ttttctgaaa gcaaactagt
     4261 tatatagtcc tcaatatttc ttctattttc attttgcttt aacttccatt cacaaatata
     4321 gtcaataata tgctcaaaag cagtctgtat accaatttga taaccatcac atacagcatc
     4381 tatacgcagt gattcagctt ttttcgtagc ctcctttata acacaaattg ctttcttctt
     4441 actctcagtg tctctatctt ttatggtttt acataattcc ttttcaattc tctttataac
     4501 aacaccatca taggcatgat gtctggatac cggtaaagtg cctttttgca tattgcatac
     4561 cttcatttaa tcgggacaaa tttctttcta caggcgtata atttccctcc aaagcaattg
     4621 gattaaataa taagtttatt ctttcatata taccaacaga tatattttcc ttccacgcaa
     4681 taagatgcca aaaaccactt cgaatagcgg cctccccttg cttactgatt ttgcccccat
     4741 aataactaat aaccatctca ctatgattaa ttaaccgaga ctcttgacta ccgataaaat
     4801 aagcaacagc tggaagtaaa ttccagttgg caacaaacat agcggttacc ccgcttaaag
     4861 gttcaatatc gcaatttag
//
LOCUS       DAZSNM010000249         4870 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZSNM010000249 DAZSNM010000000
VERSION     DAZSNM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.249"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..527
                     /gene="ydcF"
                     /locus_tag="ACHW42_003781"
     CDS             <1..527
                     /gene="ydcF"
                     /locus_tag="ACHW42_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR8945401.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="ACHW42_003782"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="ACHW42_003782"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFR8945402.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHW42_003783"
                     /pseudo
     CDS             complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHW42_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="ACHW42_003784"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="ACHW42_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR8945403.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4169..4342
                     /locus_tag="ACHW42_003785"
     CDS             4169..4342
                     /locus_tag="ACHW42_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945404.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4368..4436
                     /gene="yncP"
                     /locus_tag="ACHW42_003786"
     CDS             4368..4436
                     /gene="yncP"
                     /locus_tag="ACHW42_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR8945405.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHW42_003787"
     CDS             complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHW42_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR8945406.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4664..4804)
                     /locus_tag="ACHW42_003788"
                     /pseudo
     CDS             complement(4664..4804)
                     /locus_tag="ACHW42_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1246 a         1121 c         1209 g         1294 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     3961 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4021 gacaaataaa accataatcc ttgcccgtac gtccatctgg cttattttta atcaattcac
     4081 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac
     4141 ataagattcg acaaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     4201 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     4261 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     4321 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     4381 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     4441 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     4501 tgtgaatgcc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     4561 catagccttt ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc
     4621 attcgttggc ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat
     4681 aggtctgatt ccgatattca tttggagtca gaccttttaa tttcaggcta attcttctgc
     4741 tgttgtagta ttcaatatat tccgtaacag catccttcag ttcgcttata ttactgaact
     4801 cattagactg gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt
     4861 aatactagtt
//
LOCUS       DAZSNM010000250         4778 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZSNM010000250 DAZSNM010000000
VERSION     DAZSNM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.250"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..304
                     /locus_tag="ACHW42_003789"
     CDS             <1..304
                     /locus_tag="ACHW42_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFR8945407.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            276..2203
                     /locus_tag="ACHW42_003790"
                     /pseudo
     CDS             276..2203
                     /locus_tag="ACHW42_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2187..2390
                     /locus_tag="ACHW42_003791"
     CDS             2187..2390
                     /locus_tag="ACHW42_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFR8945408.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2387..3980
                     /locus_tag="ACHW42_003792"
                     /pseudo
     CDS             2387..3980
                     /locus_tag="ACHW42_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3970..>4778
                     /locus_tag="ACHW42_003793"
     CDS             3970..>4778
                     /locus_tag="ACHW42_003793"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR8945409.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSG"
BASE COUNT         1093 a         1121 c         1459 g         1105 t
ORIGIN      
        1 actcattgcc agatgggaac tgaccgcaga acaggctgtc gcacagcagt taaaaaatca
       61 ggtgtcaaaa ggaaatctta tcgataccgg attttgtatt tttgccctta gtaagctggc
      121 gatggcttta tccagtacgc ttgactccat tcctttgtcc atgcaacgac agttcccgga
      181 tttaacgccg cgtcatattg atcatctgaa aatccttatt gctaagggag cgaatcagtg
      241 cgcgcgtgcg ggggataaat taccggattt actcgatgaa tatatcagaa caacaactga
      301 gtaatatgat ggtggcagtc actatcgcac tgcagccact ggtcagggta ttgccggtga
      361 cggctgtaga atgggccgat caaaattatt acctgcctaa agaatcgtca tacggtgagg
      421 gggaatggaa aacgctgcag tttcaggtcg ccatcatgaa ctgcatgggc aatgacgaga
      481 ttcgtacagt taacctgatt aaatctgccc gtgttggtta tacaaaaatg gtgcttggtg
      541 tgatcgggta ttttattgag cacaaatccc gcaacactct tctttttcag ccgacggact
      601 cagctgctga agattttatg aagtctcacg tggaagcaac aattcgtgat gttccttgcc
      661 tgaaaaaact ttctccctgg ctgggccgga aacaccggga taatacgctc acactgaagc
      721 gtttttcctc tggtgtgggg ttctggtgtt tgggtggtgc tgctgccaaa aactaccgtg
      781 aaaaatctgt cgatgtggtc tgctatgacg aactctcctc gtttgaaccg gatgtggaaa
      841 aagaaggttc gccaacactg cttggcgata aacgtattga aggttcggta tggccaaaat
      901 ccatacgcgg ttcaacgcca aaaattaaag gctcctgtca gattgaaaaa gctgcgaatg
      961 aatctgcgca tttcatgcgg ttttatgtcc cttgccctca ttgcggggag gagcagtatc
     1021 tgaagtttgg cgatgatgcg acgtcgtttg gcctgaaatg ggagaagggt aaaccggaaa
     1081 cggtgtatta cctgtgtgaa cataatggct gcgtgatccg tcagtctgaa cttgatcaga
     1141 ccggggggcg ctggatttgt gacaataccg ggatgtggac gtgtgacggt ctgacgtttt
     1201 tcagtgcttc gggtaatgaa attccgccgc cgcgctccat cactttccat atatggacgg
     1261 cgtacagtcc gttcaccacc agggtacaga tagtctgtga ctggctggat gcactgaaag
     1321 atcccaacgg cgtgaaaact tttgtgaaca ccacgctggg cgagacctgg gaagaggccg
     1381 tgggcgaaaa actcgatcac caggtactga tggataaggt tgtgccttac acggcaacgg
     1441 tgcctgtccg ggtggtttat ctgacggcgg gcattgactc gcagcgaaac cgttttgaga
     1501 tgtatgtctg ggggtgggct ccgggagagg aagcctttct ggtggataaa atcatcatca
     1561 tgggacgtcc tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc aataaaaaat
     1621 accgccatgc agacggtacc gaaatgacca tttcccgtgt ctgctgggat atcgggggga
     1681 tcgatggcga aatcgtgtat cagaggtcaa aaaaacacgg tattttccgg gtactgccgg
     1741 taaaaggtgc ctctgtctat ggcaagccgg tgatcaccat gccgaaaacc cgcaatcagc
     1801 ggggtgtgta tctgtgtgag gtggggacgg ataccgcaaa agaaattctc tatgcccgaa
     1861 tgaaggccga tcccacccct gtgaatgagg ccacatcgta tgccatccgc tttcctgata
     1921 atccggagat tttttcgcag acagaggcac aacaactggt ggcggaggaa ctggtggaaa
     1981 agtgggaaaa aggaaagatg cgtctgttgt gggataacaa aaagcggcgt aacgaagcgc
     2041 tggactgcct ggtgtatgcc tatgcggcat tacgtgtgtc cgtgcaacgc tggcaacttg
     2101 atctggctgt gcagttgcat cccgcaagga tgcaactgca caaccgaccc ttgaagaact
     2161 ggcagcgaag ctgtccggag gagtgaatgg ttacagtcgc tgaactgcag gcgctgcgtc
     2221 aggcgcgcct tgatttatta accggtaaac gggttgtgtc ggtacagaaa gacggtcgca
     2281 ggatcgaata tacggcagcg tccctgaatg agcttaaccg tgcgatcaac gatgcggagt
     2341 cggttctggg tacgacccga cgccgccgac cgctgggagt gaggttatga aacgaacgcc
     2401 tgtcctgatt gatgtgaacg gcgttccgct tcgtgagagt ctcagctaca acggaggcgg
     2461 cgcaggattt ggcgggcaaa tggcagagtg gttaccaccg gcgcagagtg ccgatgcggc
     2521 cctgttgcct gcattgcgtc tggggaatgc ccgggcagat gatctggtgc gcaataacgg
     2581 tatcgcggct aatgcggtgg ccctgcataa ggatcatatc gtcgggcaca tgtttcttat
     2641 cagctaccgt ctgaactggc gttggctggg gatgcgggag accacggcaa aaagttttgt
     2701 cggggaggtg gaagcagcct ggtcggaata cgcagaagga atgtttggcg agatcgacgt
     2761 ggaagggaaa cgcacgttta cggaatttat ccgtgaaggg gtgggcgtgc atgcctttaa
     2821 cggcgaaatc tttgtgcagc cggtctggga tacggaaacc acgcagttat tccgtacgcg
     2881 ttttaaagcc gtgagtccga aacgggtgga cacgccagga cacggtatgg ggaaccactt
     2941 tctgcgggcc ggggttgagg tcgatcggta tgggcgggcg attgcgtacc atatctgtga
     3001 ggatgatttt ccgttctctg gtgccggacg ctgggagcgg atcccgcgtg agcttcccac
     3061 cgggcgtccg gccatgctgc atatttttga gccggtggag gacgggcaga cccgtggggc
     3121 taatcagttt tacagcgtaa tggagcggat gaagatgctg gactccctgc aggccacaca
     3181 gcttcagtcg gccattgtga aggcgatgta tgcagccaca atcgaaagtg aacttgatac
     3241 cgaaaaggca tttgaatata tcgcgggggc accgcaaggt cagcaggata atccgcttat
     3301 taatattctg gagaagttca cccgctggta tgacacgaat aacgtgacgc tgggcggtgt
     3361 taaaattccg cacctttttt cctggcgatg cgctgaacct gcagacagcg caggattcag
     3421 acaatggatt ttcggcgctt gaacaggcgc tgctgcggta tattgcagcc ggtcttggcg
     3481 tttcctacga acagttgtcc cgtgattact cgaaggtcag ttactcaagt gcccgcgcct
     3541 ccgccaatga gtcgtggcgc tattttatgg ggcggcgaaa atttattgcg gcccggctgg
     3601 ccacgcagat gttttcctgc tggctggaag aggcgcttct tcgggggatt attcgtccgc
     3661 cacgggcgcg ttttgatttt taccaggcgc gttcagcctg gtcacgggca gagtggatta
     3721 gctccggaag aatggccatt gacgggctca aggaggtcca ggaatcggtg atgcgcattg
     3781 aggccggact gagcacgtat gagaaagaac tggcactgat gggcgaagat tatcaggaca
     3841 ttttccgcca gcaggtcagg gaatccgcag agcgcgaaaa agccggactc tcacgtccgg
     3901 tgtggatagc ccaggcgtat cagcagcaga tagcggagag tcgcaggccg gaagaggaga
     3961 caacatcacg tgagacgtaa tctttcacat attatcgctg cggcattcaa tgagccgctg
     4021 cttctggagc ccgcctatgc gcgggttttc ttttgcgcgc tcgggcgtga gatgggcgca
     4081 gcaagccttt cggtaccgca acagcaggta cagcttgacg ctcccgggat gctggctgaa
     4141 acggacgagt acatggccgg aggcaaacga cctgcccgtg tttaccgggt ggtgaacggt
     4201 attgctgtac tgccggtgac cggcacgctg gtacaccggc tgggtggcat gcggccattt
     4261 tccggaatga ctggctatga cggcattgtc gcctgtcttc agcaggcaat ggcggacact
     4321 caggtacggg gcgtactgct ggacattgac agttctggcg ggcaggccac cggcgcgttt
     4381 gactgcgctg acatgattta ccgtctccgg cagctgaagc ctgtctgggc actgtgcaat
     4441 gacacggcct gttcagccgc catgttactg gcatcggcct gctcccgacg gctggttacc
     4501 cagacatccc gtatcggctc cattggggtg atgatgggcc atgtcagcta tgccggtcat
     4561 ctggcacagg ccggtgtgga tatcacgctg atttacgccg gatcgcacaa ggtggatggc
     4621 aatcagtttg aatccctgcc agcaaaggtt cgccaggaca tgcagcagcg cattgatgcg
     4681 gcgcgccgga tgtttgctga aaaagtggcg atgtataccg gtttatctgt ggatgcggtt
     4741 atgggaacag aggccgccgt ttttgaaggt cagtccgg
//
LOCUS       DAZSNM010000251         4758 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZSNM010000251 DAZSNM010000000
VERSION     DAZSNM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.251"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..357)
                     /locus_tag="ACHW42_003794"
     CDS             complement(<1..357)
                     /locus_tag="ACHW42_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HFR8945410.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLL"
     gene            complement(810..2072)
                     /locus_tag="ACHW42_003795"
     CDS             complement(810..2072)
                     /locus_tag="ACHW42_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8945411.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(2270..2345)
                     /locus_tag="ACHW42_003796"
     tRNA            complement(2270..2345)
                     /locus_tag="ACHW42_003796"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2310..2312),aa:Phe,seq:gaa)
     gene            complement(2451..3158)
                     /locus_tag="ACHW42_003797"
     CDS             complement(2451..3158)
                     /locus_tag="ACHW42_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR8945412.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3311..3394
                     /gene="yqgH"
                     /locus_tag="ACHW42_003798"
     CDS             3311..3394
                     /gene="yqgH"
                     /locus_tag="ACHW42_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR8945413.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            3556..>4758
                     /gene="speC"
                     /locus_tag="ACHW42_003799"
     CDS             3556..>4758
                     /gene="speC"
                     /locus_tag="ACHW42_003799"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR8945414.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNA"
BASE COUNT         1189 a         1159 c         1160 g         1250 t
ORIGIN      
        1 aagcaggtct ctgggaatgg aggtcgctgg cgtcggatat aaaaaaccct cacggcttag
       61 caaggctggc gtcactatcg gggtaacaat atcaggcgcg cctgcggaac ggtccttacc
      121 atgctgcagt aaccggaaat aaacttttgg gcgcaagatg acatcgaccg tttcgacatc
      181 gtctttttct ccctcaaaaa atttactgac aatcgcttcg tcaagacgtc ccgccgctat
      241 cccatgccag tgagtgaaat tttgggcgtc tttccgttta aaactgccct ttcctgactc
      301 agcatccgca agcgagttgc gccagtatga ggcaaaccct aaagcatttt catccattac
      361 cagtcctgtg actcaatgaa atccattgat ttatcgaata taaaatcacc gtatggacag
      421 ctattgtcca tattgaaaaa gttggatgca atgttttgtt tataattcga tatcaaatga
      481 actggttgta caaggttcag aacacaagct cattaaacag tacgtcagga ctattgattt
      541 cataacagtg aatacatttt ttaccgttta taataactac ctgtgttgga cggggatacc
      601 agagcagccg aaaacaccgg gggttattac ctgctctcat ggaaaccata tattcctggt
      661 gctaatcaca gtcataaata gcctgaagaa cgtgacgaag ggactttaat ggaagccgga
      721 gtaacttgtc agaaacatca atcttcgtca tcatttacaa agaaaaataa ttagttaaag
      781 cagtccgggc tgattgcccg gactgcttac tattttaatg gattgttaat ctttgcatat
      841 tcaaacgggc tgataccctt ctcccggttc gcatctagaa aatcggccca ccattgcaac
      901 atcagtcgcc gttcttccag atgttctgct ttatggatat acgcagcacg tacaccatta
      961 cgctcctgat ggctcatctg acgttccaca gcatcgcggg accacaaacc tgattcaatc
     1021 aatgcactgc acgccatcgc ccggaatcca tggccacaga cttcctgggt tgtgtcatac
     1081 cccatcaccc tcagggcttt atttacagta ttttcactga ttggtttacg cgggtcatgt
     1141 gcacccgtaa agatcagacc attttcacca gcccatgtct gaagttctgc cagtatggct
     1201 acaacctgtt tgctgagtgg cacaagatgt ggtgttcgca tcttggatcc ccggtgtgag
     1261 aattttacgc ctggcagagg ttcgcgttcg gctggaatag tccacatgga attgtcaata
     1321 tcaatttccg accagcgggc atatctgagt tcactggaac gaataaaaac cagtaaattc
     1381 agcataactg ccagtcgggt gacaggctgg cctttataac cgtcaatacg cgacagaagt
     1441 tcaggcaggc gtgaaagctc cagagcaggg cgatgctggc gttttaccgt ggtcaatgca
     1501 ccagccatat ccagagccgg gttatatctg atgatgccag actgtacggc gtaacgcatt
     1561 acagcagata ttctttgctg cagacgactg gcgatttcat aaatttgttt tgcttctgca
     1621 gcacgaacag ggataagcag gtccggggta ttgagtgttg tgatatcccg gttgccgata
     1681 aaggggaaaa cgtgtgtctc gaggcttttg agtactttat cgctgtgtga ttgtgcccat
     1741 tgtttattac tggcatgcca gcgtcgtgca atatgttcaa aagagagctg taaatcaggg
     1801 acaacttttt taactctttt ctgttcgcaa gggtcaatcc cctctgaaat cagttttcgg
     1861 gcctcatccc gttttgtacg agcttcggag agagaaactt ctggatacac accaagtgcc
     1921 agagtcttct ccttacccag aatacgataa cggagacgcc agtaacggga accattagga
     1981 tgaaccagca ggaacatgcc ggccccgtca gtgagtttat aagccttgtc agtgggcttt
     2041 gcagcccgga tttttgcgtc agtcagtgcc atagttcagc cttctgaatg cgttaaagag
     2101 ggtacaaatt ctgtcgaact ggggtatacc cgcaattata cccacacaat ggggttgatg
     2161 tggggggaat ctggttgagt tcggtagaat tgatttggag gaagaacact taaatcatgg
     2221 tgttctgaaa cgaaaaacgg acctccgtgg aggtccgttg atatgaattt ggtgcccgga
     2281 ctcggaatcg aaccaaggac acggggattt tcaatcccct gctctaccga ctgagctatc
     2341 cgggcaacgg ggcgcattaa acctgattcg cctcgtctcg tcaatcaaat ttcttcaatt
     2401 tactgcagac tgcacaatct atcatcactt tgccgctatt gcacgcattg tcaggcaaac
     2461 caggcggtcc aggcggcgga aagcggcatt gccagaagga gtgctggaag catgttgacc
     2521 accgggaaca ttttaatgcc acagatgcgt aatccggttg ccagcagcaa taaaccgcct
     2581 acggcgctga agtctgccat catcgacggt gtgatcagcg gtaatatcag cgcggcagcc
     2641 cacgccagcg ttaactggat gatcagtaat gggatactaa ttaccgacac cgcaataccc
     2701 agcgagcagg cgaagatcat cgccgtaaag aaatcaagaa atgacttggc gattaaaata
     2761 ctcggatcgc cggtcatccc ttcgttcatc gccccgaaga tcccggtgcc gctggcgcaa
     2821 aacaggacaa taatcgcgac ataattctga ataaaagatt catgcgctgg cttcttacgt
     2881 gagtgacgaa acagattttg tgctttggcg actgctgtat tgacaccttt ctccagcaaa
     2941 caaatttcgc caattagagc gccaagtagc gtagctaaaa ccatcgctgg aaggttggcg
     3001 catttcacaa ccagtaaaat accaatcccc agcgatgcca gaccaaaaat agatgtcatg
     3061 gagacgcgga tacgttccgg taagcgttgg ctgagcagtg cgccgagaac gccacccagt
     3121 aagactgcac tagcgttgat aaaagggccg atgaccacaa gagttcctgt tagctgtatt
     3181 gtttgatttt cttattatga actttttgcc ttgttgatgg cttgtctggt cgcaaagtcc
     3241 tggcttgcac gccttagcga aaggtgccat gattgcgcga attttctcct ctctgtacgg
     3301 agtttgcccg atgcacgcca tctccttaca ttctctcgct tatcgccgtt tcgcgcgaaa
     3361 cgtttccctt tctatgttac tgctcatgcg gtgaagttaa cgcacgctca ctgcaggaca
     3421 acagtaaaat cagagcgttt ctgcttttac tgatgtctgg cggtcggagc tggtgaccag
     3481 tttgacccat atctcatggg gcagggtttt ccaccttgtc cggtattctt acttccccga
     3541 aacgggtttg cgcttatgaa atcaatgaat attgccgcca gtagtgaact ggtatcccga
     3601 ctttcttctc atcgccgcgt ggtggcgttg ggagatactg attttacgga cgtcgcggca
     3661 gtcgtcatta ccgctgcgga tagtcgcagt ggcattcttg cgttgcttaa gcgcaccggt
     3721 tttcatctac cggtgttttt gtattccgaa catgctgttg aattacctgc gggcgttacg
     3781 gcggtaatca acggcaacga gcagcagtgg ctggagctgg aatccgcagc ctgtcagtat
     3841 gaagagaatt tgctgccacc gttttatgac acgctgacgc agtacgttga gatgggcaac
     3901 agcacctttg cttgccctgg acatcaacat ggtgcgttct ttaaaaagca tcctgccgga
     3961 cgccattttt acgatttctt tggtgagaac gtctttcgcg ccgatatgtg taacgctgac
     4021 gtaaaattgg gcgatctgct tattcatgaa ggatcggcga aagatgcgca gaaattcgca
     4081 gccaaagtct ttcatgccga taaaacctat tttgtgctga acggcacatc ggcagcgaat
     4141 aaagtggtga cgaatgcgct gttaacgcgt ggcgatctgg tgctcttcga ccgtaacaac
     4201 cataagtcga atcatcacgg cgcgctgatt caggcgggga cgacgccggt ctatctggaa
     4261 gcttcacgta acccgtttgg tttcattggc ggtattgatg cgcactgttt taatgaagag
     4321 tatctgcgcc agcaaattcg cgacgttgcg ccagaaaaag ccgacctgcc gcgcccgttt
     4381 cgcctgacga ttattcagct gggaacctat gacggcactg tctataacgc ccgtcaggtg
     4441 atcgatacca ttgggcatct gtgtgattac attctgtttg attccgcgtg ggtcggttac
     4501 gaacagttta tctcgatgat ggcggatagc tcgccgctgc tgttagaact taacgaaaac
     4561 gatccgggga tttttgtgac ccagttggtg cacaaacagc aggctggatt ctcacagacg
     4621 tcgcagatcc ataaaaaaga taaccatatc cgcggacagg cgcgtttttg cccgcataag
     4681 cggttgaata acgcctttat gctccatgct tctaccagcc ctttctatcc gctgtttgct
     4741 gcactggatg ttaacgcc
//
LOCUS       DAZSNM010000252         4750 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZSNM010000252 DAZSNM010000000
VERSION     DAZSNM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.252"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..746
                     /locus_tag="ACHW42_003800"
     CDS             <1..746
                     /locus_tag="ACHW42_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8945415.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="ACHW42_003801"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="ACHW42_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR8945416.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3606..3809
                     /gene="safA"
                     /locus_tag="ACHW42_003802"
     CDS             3606..3809
                     /gene="safA"
                     /locus_tag="ACHW42_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR8945417.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3877..4638
                     /gene="ydeO"
                     /locus_tag="ACHW42_003803"
     CDS             3877..4638
                     /gene="ydeO"
                     /locus_tag="ACHW42_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR8945418.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT         1344 a         1027 c         1087 g         1292 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaatgca tgcgaccaca gtgaaaaaca aaatcacgca aagagataac tataaagaaa
     3661 tcatgtctgt aattgtggtt gtcttattac tgacacttac gttgatagcc attttttcgg
     3721 caattgatca gctgagtatt tcagaaatgg gtcgcattgc aagagatctt acacatttca
     3781 ttatcaatag tttgcaagac tggaaataaa cagcaaatta taaatgtgaa atgtttaaaa
     3841 ggtatcgata aaactttatt gttttaagga gataaaatgt cgctcgtttg ttctgttata
     3901 tttattcatc atgctttcaa cgctaacatt ttagataaag attacgcctt ctccgacggc
     3961 gagatcctga tggtagataa cgctgttcgt acacattttg aaccttatga acggcatttt
     4021 aaagaaatcg gatttaatga aaataccatt aaaaaatatc tacaatgcac taacatccag
     4081 acagtgacgg tgcctgttcc tgcgaagttt ttacgtgctt caaatgtacc gactggattg
     4141 cttaatgaaa tgattgctta tctcaactcg gaagaacgca atcatcataa tttttcagaa
     4201 cttttgcttt tttcttgcct gtctattttt gccgcatgca aaggtttcat tacactatta
     4261 actaacggtg tgctatccgt ttccgggaaa gtgagaaata ttgtcaacat gaagctggcg
     4321 cacccatgga agctgaaaga tatttgtgac tgcctgtaca tcagtgaaag cctgttgaag
     4381 aaaaaactaa agcaagagca aacgacattc tcacagattc tattagatgc cagaatgcag
     4441 cacgcaaaaa atttgatacg cgtagaaggt tcagtcaata aaattgccga acaatgtggt
     4501 tatgccagta catcttattt tatttatgcg ttccgcaaac atttcggcaa cagtccgaag
     4561 agagtttcta aggagtaccg ttgtcaaagg cacacgggta tgaatacggg caacacgatg
     4621 agtgctttag ctatttgatt atttgctaac gagtagtcaa ccacgcacgc tacgtaagaa
     4681 ttatatgggg cagccattcc ctgccccgcg ttgtttttag gcgatatatt tattgaaata
     4741 aataagtgac
//
LOCUS       DAZSNM010000253         4688 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZSNM010000253 DAZSNM010000000
VERSION     DAZSNM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.253"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            95..1135
                     /gene="pstS"
                     /locus_tag="ACHW42_003804"
     CDS             95..1135
                     /gene="pstS"
                     /locus_tag="ACHW42_003804"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR8945419.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            1222..2181
                     /gene="pstC"
                     /locus_tag="ACHW42_003805"
     CDS             1222..2181
                     /gene="pstC"
                     /locus_tag="ACHW42_003805"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR8945420.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            2181..3071
                     /gene="pstA"
                     /locus_tag="ACHW42_003806"
     CDS             2181..3071
                     /gene="pstA"
                     /locus_tag="ACHW42_003806"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR8945421.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            3163..3936
                     /gene="pstB"
                     /locus_tag="ACHW42_003807"
     CDS             3163..3936
                     /gene="pstB"
                     /locus_tag="ACHW42_003807"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR8945422.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            3951..4676
                     /gene="phoU"
                     /locus_tag="ACHW42_003808"
     CDS             3951..4676
                     /gene="phoU"
                     /locus_tag="ACHW42_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR8945423.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1080 a         1192 c         1299 g         1117 t
ORIGIN      
        1 tgtcatattc cttatatata actgtcacct gtttgtccta ttttgcttct cgtagccaac
       61 aaacaatgct ttatgaatcc tcccaggaga cattatgaaa gttatgcgta ccaccgtcgc
      121 aactgttgtc gccgcgacct tatcgatgag tgctttctct gtgtttgcag aagcaagcct
      181 gacaggtgca ggtgcaacct tccctgcgcc ggtgtatgcc aaatgggctg acacttacca
      241 gaaagaaacc ggtaataaag ttaactacca gggtatcggt tcttccggtg gcgtaaaaca
      301 gattatcgct aataccgttg attttggtgc ctctgacgcg ccgctgtctg acgaaaaact
      361 ggctcaggaa ggtctgttcc agttcccgac cgtgattggc ggcgtggtgc tggcggttaa
      421 cattccaggg ctgaagtctg gcgaactggt gctggatggt aaaaccctcg gcgacatcta
      481 cctgggcaaa atcaagaagt gggatgatga agccatcgcc aaactgaatc cgggtctgaa
      541 actgccttca caaaacattg ctgtagtacg ccgcgcagat ggctccggga cttccttcgt
      601 cttcaccagc tacctggcga aagtgaacga agagtggaaa aacaacgttg gtactggctc
      661 taccgtaaaa tggccgatcg gtctgggcgg taaaggtaac gacggtatcg ccgcgttcgt
      721 tcagcgtctg ccgggtgcaa ttggttatgt tgaatatgct tacgcgaagc agaacaacct
      781 ggcgtacacc aaactgatct ccgctgatgg taaaccggtt agtccgaccg aagaaaactt
      841 cgctaatgca gcaaaaggtg cagactggag caaaaccttc gctcaggatc tgaccaacca
      901 gaaaggcgaa gatgcatggc ctattacctc taccacgttc attctgatcc acaaagatca
      961 gaagaaacca gaacaaggca cagaggtgct gaaattcttc gactgggcgt acaaaaccgg
     1021 ggctaaacag gcgaacgacc tggattacgc cagcctgccg gatagtgtag ttgaacaggt
     1081 tcgcgctgcg tggaagacca atattaaaga cagtagcggt aagccgctgt actaataaaa
     1141 ctccaggccg ggtacggtgt tttacgccgc atccggcatt acaaaatgac tttgtaaacg
     1201 cgtttaactg aagagtaact tatggctgca accaagcccg cttttaaccc accgggtaaa
     1261 aagggcgaca taattttcag cgtgctggta aaactggcgg cgctgattgt gctattgatg
     1321 ttgggtggca ttattgtctc tctgatcatc tcctcctggc cgagcattca gaaatttggt
     1381 ctggctttcc tatggaccaa agagtgggat gcaccgaacg atatctacgg ggcgctggtg
     1441 ccgatctacg gtacgttggt gacttcgttt atcgcgctgc tgatcgccgt cccggtgagt
     1501 ttcggtatcg ccctgttcct gactgagctt gcgcctggct ggctgaaacg cccgctgggt
     1561 atcgccattg agctgctggc agccattcca agtatcgttt acggcatgtg gggcctgttt
     1621 atctttgcgc cgctgttcgc cgtttacttt caggagccgg tcggcaatat catgtcgaat
     1681 atcccgattg ttggcgcgct gttctctggc cccgcatttg gtatcggtat cctcgcggca
     1741 ggcgtgatcc tcgccatcat gattattccg tacattgcgg cggtaatgcg tgatgtgttc
     1801 gaacaaaccc cggtgatgat gaaagagtcg gcctacggta ttggctgcac cacctgggaa
     1861 gttatctggc gtatcgttct tccgttcacc aaaaatggtg ttatcggcgg catcatgctg
     1921 gggctgggcc gcgcgctcgg tgaaaccatg gcggtgacct ttatcatcgg taacacctac
     1981 cagctcgaca gcgcctcgct gtacatgccg ggcaatagta tcacctctgc gctggcgaac
     2041 gaatttgcgg aagcggaatc cggtctgcac gttgccgcac tgatggaact gggcctgatc
     2101 ctgtttgtga ttaccttcat cgtcctcgcc gcatcgaagt ttatgattat gcgcctggct
     2161 aagaatgagg gggcacgcta atggctatgg ttgaaatgca aaccactgcg gcgctggctg
     2221 aatctcgccg caaaatgcag gcgcgtcgcc gcctcaaaaa ccgtattgcg ctgacgctct
     2281 cgatggcgac gatggccttc ggcctgttct ggctgatctg gattttaatg tccaccatca
     2341 ctcgcggtat cgacggtatg tcgctggcgc tgttcactga aatgacgccg ccgcccaata
     2401 cggaaggtgg tggtctggcg aacgccctgg cgggtagcgg gctgttaatt ttgtgggcca
     2461 cggtattcgg tacgccgctg ggcattatgg cggggattta tctggcggaa tatggtcgta
     2521 aatcctggct ggcagaagtg attcgcttca ttaacgacat tctgctctcc gcgccgtcga
     2581 ttgtggttgg tctgtttgtc tacaccatcg tggtggcaca gatggagcac ttctccggct
     2641 gggcgggcgt aattgcgctg gcgctgttgc aggtgccgat cgttatccgc accactgaga
     2701 acatgctgaa actggtgccg gacagcctgc gtgaagcggc ttatgcgctg ggtacaccga
     2761 agtggaagat gatctccgcg attacgctga aagcgtcggt gtccgggatt atgaccggta
     2821 tcctgctggc gattgcccgt attgccggtg aaaccgcgcc gctgctgttt accgcgctct
     2881 ccaaccagtt ctggagcacg gacatgatgc agccgatcgc caacctgccg gtgacgatct
     2941 ttaagtttgc gatgagcccg tttgcggaat ggcaacaact ggcctgggcc ggggtattga
     3001 tcattaccct gtgcgtcctg ctgctgaaca ttctggcgcg cgttgttttt gcgaagaata
     3061 aacacggtta acacttttcc ggatgcggcg tgaacgcctt acccggccct acggtaagcc
     3121 tgattagcga aaagcatcag gtacgatgag gaaaagattg caatgagtat ggttgaaact
     3181 gccccgagta aaattcaggt tcgtaatttg aacttctact acggcaaatt ccatgccctg
     3241 aaaaacatca acctggatat cgccaaaaac caggttacgg cgtttatcgg gccgtccggc
     3301 tgcggtaaat cgacgctgct gcgtaccttc aacaaaatgt ttgaactgta cccggagcag
     3361 cgtgcggaag gtgaaattct gcttgatggc gacaacatcc tgaccaactc tcaggatatc
     3421 gccctgctgc gtgcgaaagt gggcatggtg ttccagaaac cgacgccgtt cccgatgtcc
     3481 atctacgaca acatcgcctt tggcgttcgt ctgtttgaga agctctcccg tgccgacatg
     3541 gacgagcgcg tgcagtgggc attgaccaaa gccgcattgt ggaacgaaac caaagataaa
     3601 ttgcaccaga gcggttactc tctctctggt ggtcagcaac agcgtctgtg tattgcgcgt
     3661 ggtatcgcca ttcgcccgga agtgctgctg ctcgacgaac cgtgttcggc gctcgacccc
     3721 atctctaccg ggcgtattga agagctgatc accgaactga agcaggatta caccgtggtg
     3781 atcgtcaccc acaacatgca gcaggctgcg cgttgttccg accacacggc atttatgtac
     3841 ctgggcgaat tgattgagtt cagcaacacg gacgatctgt tcaccaagcc agcgaagaaa
     3901 caaacagaag actacatcac cggtcgttac ggttgattca ggagtgcgtt atggacagtc
     3961 tcaatcttaa taaacatatt tccggccagt tcaacgccga actggaaagt atccgcacgc
     4021 aggtgatgac catgggcggc atggtggagc agcagctttc tgatgcaatc accgcgatgc
     4081 ataaccagga cagcgatctg gcgaagcgcg tgatcgaagg cgacaagaac gtcaacatga
     4141 tggaagtggc gatcgatgaa gcctgcgtgc gcattatcgc caaacgtcag ccgacggcga
     4201 gcgacctgcg actggttatg gtgatcagta aaaccattgc cgagctggag cgtattggcg
     4261 acgtggcgga caaaatctgc cgtactgcgc tggagaaatt ctcccagcag catcagccgt
     4321 tgctggtaag tctggagtcg ctgggccgtc ataccatcca gatgctgcac gacgtgctgg
     4381 acgcgttcgc gcggatggac attgacgaag cggtacgtat ttatcgtgaa gataaaaaag
     4441 tcgatcagga atacgaaggt attgttcgtc aactgatgac ctacatgatg gaagattcgc
     4501 gcactattcc gagcgtactt accgcgctgt tctgtgcgcg ttcaatcgaa cgtatcggcg
     4561 accgctgcca gaatatttgt gagtttatct tctactacgt gaagggtcag gatttccgtc
     4621 atgtaggcgg tgatgagctg gataaactgc tggcgggaaa agatagcgac aaataattca
     4681 ccagacaa
//
LOCUS       DAZSNM010000254         4602 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZSNM010000254 DAZSNM010000000
VERSION     DAZSNM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.254"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(78..866)
                     /gene="phoH"
                     /locus_tag="ACHW42_003809"
     CDS             complement(78..866)
                     /gene="phoH"
                     /locus_tag="ACHW42_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR8945424.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="ACHW42_003810"
     CDS             complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="ACHW42_003810"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR8945425.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="ACHW42_003811"
     CDS             complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="ACHW42_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR8945426.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3929..>4602)
                     /gene="efeU"
                     /locus_tag="ACHW42_003812"
     CDS             complement(3929..>4602)
                     /gene="efeU"
                     /locus_tag="ACHW42_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR8945427.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1116 t
ORIGIN      
        1 ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct
       61 ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt
      121 acgcaatcct cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc
      181 agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg
      241 ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca
      301 gtcacattct gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata
      361 taggcgaacg gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag
      421 gaagccccta agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt
      481 tctgcgatat caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg
      541 acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc
      601 caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc
      661 tgcagatagt gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg
      721 cgggccatac caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct
      781 tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct
      841 ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac
      901 gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca
      961 tagtttgctt ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta
     1021 acacgagtaa agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga
     1081 aatctccgcg agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca
     1141 tccgcgatct ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag
     1201 tacaacagaa ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca
     1261 tttcggtgac attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc
     1321 tgtgtgcttt ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg
     1381 gcgcgaacgc cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg
     1441 ataaacctgc gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga
     1501 taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta
     1561 acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc
     1621 agatcgtgtt ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc
     1681 cccaacgaat aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg
     1741 ggattcgcca gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg
     1801 taatcaggca catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg
     1861 ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga
     1921 atcaagcgta ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc
     1981 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     2041 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     2101 gaaataaacc cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata
     2161 tcgcgcagcg catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca
     2221 ccatgacata acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt
     2281 tttggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac
     2341 gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc
     2401 aggcgcggat ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga
     2461 gtcaacaagc gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc
     2521 accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac
     2581 ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt
     2641 gcatgagcga ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     2701 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     2761 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc
     2821 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     2881 gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt
     2941 tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga
     3001 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca
     3061 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     3121 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
     3181 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     3241 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     3301 agaacagttc agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg
     3361 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     3421 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     3481 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     3541 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     3601 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     3661 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     3721 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     3781 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     3841 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     3901 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     3961 gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt
     4021 cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg
     4081 agtgagttga gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc
     4141 cggcttcatg aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa
     4201 acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca
     4261 ggaagccgag caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc
     4321 cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa
     4381 cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc
     4441 tatcgactgc ctgttccagt tgcactttga cgttgcgcga cactttgcgc atccagaaaa
     4501 ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct
     4561 ctttttgcgg aaattcgccg gtggtttcgt taatgaagat cc
//
LOCUS       DAZSNM010000255         4533 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZSNM010000255 DAZSNM010000000
VERSION     DAZSNM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.255"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1022)
                     /gene="mltC"
                     /locus_tag="ACHW42_003813"
     CDS             complement(<1..1022)
                     /gene="mltC"
                     /locus_tag="ACHW42_003813"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR8945428.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSA"
     gene            complement(1087..1362)
                     /locus_tag="ACHW42_003814"
     CDS             complement(1087..1362)
                     /locus_tag="ACHW42_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR8945429.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(1390..2442)
                     /gene="mutY"
                     /locus_tag="ACHW42_003815"
     CDS             complement(1390..2442)
                     /gene="mutY"
                     /locus_tag="ACHW42_003815"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR8945430.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            2603..3322
                     /gene="trmB"
                     /locus_tag="ACHW42_003816"
     CDS             2603..3322
                     /gene="trmB"
                     /locus_tag="ACHW42_003816"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR8945431.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3322..3648
                     /locus_tag="ACHW42_003817"
     CDS             3322..3648
                     /locus_tag="ACHW42_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR8945432.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            3832..>4533
                     /locus_tag="ACHW42_003818"
     CDS             3832..>4533
                     /locus_tag="ACHW42_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR8945433.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKAL"
BASE COUNT         1094 a         1136 c         1237 g         1066 t
ORIGIN      
        1 tccgcagagg gatggcgggt cgtcagcgtc tgataaacat cgcccggcgt catggtgtta
       61 ataatattgg cagcctgaat cttatcattc gaaaagactc gcagcacgct gcctgcgccg
      121 ccgttatagg cggtgatgac ggcataacgc cgcgatgttg ggttatcaat tccgccgaga
      181 taaacattgt tcagcatcgc cagatacgcg gtgccggtat caatattgct ggcaggatca
      241 aacaagaaac tgcggctcgg cgtgccggat ttcccctgcg agcggaacac atctttcccg
      301 gcagtatttt gtaccacctg cattaatccc agcgcatcgg aacggctgac cgcatacggg
      361 ttaaaggaag attcagtctg cataattgcc agaatcagcg actcatcaac gccatatttc
      421 cgtgacgcct ggcggaccat gccgagatat ttgtgcgcac gtttatcaag gtggttcggc
      481 accatgttaa tggtgacgct gtagatgata cgtagcccgt tgctgcggct tttcagacgg
      541 tttttcagca gataatccgc gaagttgctt gcgcgacctt cccagcgaat cggctgcccg
      601 gtgttgtcca ccacctgacc gtaaaggaaa ggttctttcg aaattgtaat atcatcaacg
      661 tcggaataga gatcgaccga actcggatcg tcacccatca gcaacgtttt gataattgcc
      721 cggcgcaaat gcgcggcagg ttctgtcccg gcgatggttt cgatagtaat cgtaccgtca
      781 tcgaagttga tgtggctgcg ggtctgatat tgatcggtgt atttcacgta gtccttagga
      841 ccggcgatca ccacctcttt gaagccccag atgttctcaa tattgtgggc aaattgcccc
      901 atcagaatat caaaaccgtt ggtatctttg acccaggctt cgttataggt atcgcctttt
      961 ttggtcgtcg aacaggagat gagcaacggc gcaatcaaag ccagcgcgag atattttttc
     1021 atcattccgg gtgcgtgttg tgtttgtttt tatgcaagct gccggaggcg tgctccggca
     1081 ctgtttttat tttttatctt ccggcgtata gccctcgata tgcacctctt taccctcgaa
     1141 caggaagttg accatctcct gctccagcag cttgcggtgc tcggcattca tcatgttgag
     1201 tttcttttca ttaatcagca tggtttgttt gtgctgccac tgcgcccagg cttctttgga
     1261 gatctcgtta tagatgcgtt ttcccagctc gccggggtac agctgaaaat cctgaccttc
     1321 tgcttcacgt tgcaggaaag tacaaaaaat cgttctgctc ataaatcatc ctctttatcg
     1381 actcacgcgc taaaccggcg cgccagtgcg taactgctgt aacaaacgct ccacgggagc
     1441 cgccaggcca actgacggcg gttgcgctaa gttataccag agcgcattgc cttcatccat
     1501 gcagccggtg aatgacgaca cgggaagcca cataggcaca atatctaagt ggaaatggct
     1561 gaaggtatgg cgaaacgcgg tcagttgcgt caggttatcg gcagcaatct gccgttgtgc
     1621 cagccaatgc cgcaaacttt cttcgtcggc aaactgcggg aaacagtata aaccgcccca
     1681 caatccgctc ggcggacgct gcgccagcaa tacttcatct tcgtgctgta acaggagaaa
     1741 gtagccggtg cgctccggca gcgtctgttt cggttttttg cccggataaa gcgaccagct
     1801 attgttggtg gcggcaatac atccgttttg tagcggacag agcgaacatt tcggcttcga
     1861 acgcgtgcaa atcatcgcgc ccaaatccat catcgcctga ttaaaccgtt ccacgccgac
     1921 cgcgggcgtc acctgctcgc tcaaactcca tagtttattc tcgacctctt ttttcccagg
     1981 ccagccgctt acagcatagc agcgcgccag cacgcgtttg acgttaccgt cgagaatcgg
     2041 aaagtgctta cccagagaaa gcgagagaat cgcgcctgcg gtggaacgcc cgacgcccgg
     2101 taacgccgcg acttcttcaa aggtttccgg gaatttacca ccgtgtaagg tcgccacttg
     2161 ttgtgccgct ttatgcaggt tgcgcgcgcg ggcgtaatag ccaagcccgg tccacaagtg
     2221 gagaacttcg tcgagcggcg cattggcgag atcggtcacc gtcgggaagt gcgccataaa
     2281 gcgttcaaaa taggggataa cggtcgcaac ctgagtttgt tgcaacatca cttctgagag
     2341 ccatactttg tagggcgtct tgtcaatttg ccagggcagc gttttccgcc cgtatttatc
     2401 gtaccagtcc agaacctggg ctgaaaattg cgacgcttgc atggtcaccg acttcactgt
     2461 tgttgggggc aagattgcag cacagcgacg gcagggtgta aaccggaact ttccgcagcc
     2521 acggccttat catgcttgca tccggcaact aactttggat aatgcccgtt ttcagaacac
     2581 tttcacaagc gactaaatct ttatgaaaaa cgacgtcatt tcaccggaat ttgatgaaaa
     2641 cggccgccca ctgcgccgta tccgtagttt tgtgcgccgc caggggcgac tgaccaaagg
     2701 ccaggaacat gcgctggaaa actactggcc ggtgatgggc gttgagttca gtgaagatat
     2761 gctggatttc cccgcgcttt ttggccgtga agcgccggtg acgcttgaga ttggttttgg
     2821 catgggggcg tcgctggtgg caatggctaa agatcgcccc gagcaggact tcctcggcat
     2881 tgaagtgcat tcaccgggcg ttggtgcgtg cctggcttct gcgcatgaag aaggtttaag
     2941 caacctgcgc gtgatgtgtc acgatgcggt tgaagtgctg cataaaatga ttcctgacaa
     3001 ttcattgcgc atggtgcagc tctttttccc tgacccgtgg cacaaagcgc gccataataa
     3061 acgccgtatc gttcaggtgc cgtttgccga actggtaaaa agcaaactgc agctgggggg
     3121 cgtattccat atggcgaccg actgggaacc ttatgcggaa catatgcttg aagtgatgtc
     3181 ttctattgac ggttataaaa acctgtcaga gagcaatgat tacgtaccgc gtccggcatc
     3241 acgtccggtg acgaaatttg aacaacgtgg tcatcgtctt ggtcacggag tatgggactt
     3301 aatgttcgag agggtgaaat aatggcaaag aaccgtagcc gtcgtctgcg taaaaaaatg
     3361 cacatcgacg aattccagga attaggattt tcggtggcat ggcgattccc ggaaggtaca
     3421 tcggaagaac agattgataa aatcgttgat gactttatta acgaggttat cgaaccgaac
     3481 aaactggcct ttgacggcag cggttatctg gcctgggaag gtctgatctg catgcaggaa
     3541 atcggcaaat gcaccgaaga acatcaggcg attgtgcgta agtggctgga agagtgcaaa
     3601 ctggatgagg tacgcaccag cgaacttttc gacgtttggt gggactaaga aagcatacgg
     3661 gcgatgacaa atgcaaaact gcctgatgcg ctacgcttat caggcctaca gagatgcacg
     3721 atcgagtagg cgggataagg cgtttacgcc gcatccggca tggaaaacgc gtactttgtt
     3781 atcaatctgg ggccagcata tgctggcctg atttgttctt gagggaagac tatgatgcgc
     3841 aaaatgctgc tggcggcagc actttcagtg acggcaatga ccgctcacgc cgactaccag
     3901 tgcagcgtca cgccgcgtga cgatgtgatt gtcagcccgc aaaccgtgca ggtgaagggc
     3961 gaaaacggca atctggtgat cacgccagac ggcaacgtga tgtataacgg taagcaatat
     4021 tccctgaatg ccgcccagcg cgagcaggcg aaggattatc aggctgaact acgtagcacg
     4081 ctgccgtgga ttgatgaagg cgcgaaaagc cgcgtcgaga aagcccgtat tgcgctggat
     4141 aaaattatcg ttcaggagat gggcgacagc agcaaaatgc gcagccgtct gaccaaactt
     4201 gatgcgcagc ttaaagagca gatgaatcgc attatcgaaa cgcgcagcga tggcctgacg
     4261 ttccactata aagccattga tcaggttcgc gccgaaggcc agcaactagt gaatcaggca
     4321 atgggcggaa ttttacagga cagcattaat gaaatgggcg cgaaagcggt gctgaaaagc
     4381 ggcggtaacc cattacagaa tgtgctggga agcttgggcg gtctgcaatc ctcaatccaa
     4441 accgagtgga aaaagcagga aaaagatttc cagcagtttg gcaaagatgt ttgtagccgc
     4501 gttgtgactc tggaagatag ccgcaaagcc ctg
//
LOCUS       DAZSNM010000256         4502 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZSNM010000256 DAZSNM010000000
VERSION     DAZSNM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.256"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..508
                     /locus_tag="ACHW42_003819"
     CDS             <1..508
                     /locus_tag="ACHW42_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR8945434.1"
                     /translation="FREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGF
                     TQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIR
                     HNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQ
                     YSRAWTVK"
     gene            complement(509..1669)
                     /gene="ybdL"
                     /locus_tag="ACHW42_003820"
     CDS             complement(509..1669)
                     /gene="ybdL"
                     /locus_tag="ACHW42_003820"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR8945435.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1778..2866
                     /gene="hcxA"
                     /locus_tag="ACHW42_003821"
     CDS             1778..2866
                     /gene="hcxA"
                     /locus_tag="ACHW42_003821"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR8945436.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2876..3073)
                     /locus_tag="ACHW42_003822"
     CDS             complement(2876..3073)
                     /locus_tag="ACHW42_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR8945437.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3255..>4502)
                     /locus_tag="ACHW42_003823"
     CDS             complement(3255..>4502)
                     /locus_tag="ACHW42_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFR8945438.1"
                     /translation="KIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLF
                     ITIACGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPG
                     VYFAMNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGG
                     APTLAVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGV
                     VSPGLKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMA
                     LMLCAVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAM
                     IDSGNIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTA
                     KETPYEPMPENVEEIVAQAKGAH"
BASE COUNT         1099 a         1260 c         1169 g          974 t
ORIGIN      
        1 ctttcgtgaa aagccagtgg attgcgttct gtgggttaaa aacagccaac ttatgcctaa
       61 tgattacaac ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgcaaa aatccattga
      121 aatagatggt tttacccaac ccattgtggt cacacatacg gataaaaatg ccatggaaat
      181 cgtggatggt tttcaccgcc atgaaattgg taaaggctca tcatcattaa aattacgctt
      241 aaaaggttat ttgccggtga cctgtctgga aggtacgcgt aatcaacgca tcgccgcgac
      301 tattcgtcat aatcgcgctc gtggacgcca tcaaatcacc gccatgtcgg aaatcgtccg
      361 tgaacttagc cagttgggat gggatgataa taaaattggc aaagagctgg gaatggacag
      421 cgacgaagtg ctgcgactga agcaaattaa cggcctgcaa gagttgtttg ccgaccgtca
      481 atattcccgc gcctggacgg ttaaatagct aaagctggcg caggcgttca gctgctgcca
      541 gcaacgtcga ttccttcttg gcaaaacaga gacgaatcag tttatgtggg aagggatcgg
      601 cgcaaaacac cgacagcgga atcgccgcta cgccgtgctc ctgcgtcagc cactggcaaa
      661 actcaacatc atccagggta gaaaccgcgc tgtaatccac cagcaaaaag tatgtacctt
      721 cacacggtaa aatctccagc cggctttcat ttaaagcatt caccagaata tcgcgcttct
      781 ggcgataaaa gtccggtaac gcaagataat gctcaggttc tgcacgtagc atatcagcaa
      841 gcgccagctg tgccggggta ttcaccgaaa aggtcagata ctgatgtacc ttgcgaattt
      901 cggcgctgat gggcgctggc gcaacacaat aacccacttt ccagccggtc atatgatagg
      961 tcttgccaaa tgaagaaacc gccactgccc gctcacgcag ctgcggatgc gccagcacac
     1021 tggcatggcc ctgttgtgaa aagttgatgt gctcgtagac ttcatcgcta atgacaaaaa
     1081 tctcgtgccc ggcgatcgcc tgccacaaag cggcgaaatc agcctgctgc cagacagttg
     1141 cactggggtt atgcggagtg ttgaggatca ccagtctggt gcgctcgctt aataatgcgg
     1201 caaattcctg ccagtcaacg cgaaaatgcg gtggttgcag tgccatacgt ttcactattc
     1261 ccccagaaag cgcgatggcg ggggcgtaac tgtcatagct gggatcaaaa caaatcactt
     1321 catcgccatt gcgcaccagt gcggtaatcg ccgcgtataa cgcttccgtc gcccctgccg
     1381 ttacggtgat atcgctatcg gcatctggtt gatagccata caaacgttcc gttttctgag
     1441 caatcgcctc gcgcaaggcc tgcacgccgg tcatgggcgc gtattggttt gccccctggg
     1501 caacgtggta cgccagccgc tcctgtaaat agcgcggacc atcaaaatca ggaaagcctt
     1561 gcgacaggtt aatcgcctgg tgttgctgcg ccagcgcgct catctgggtg aaaatagtgg
     1621 tgccaagttg tggaagtttg ctttgtggaa tcagagggtt atttgtcatt tttattgtac
     1681 ctgacgaagt tgcgtgttga aggcactata acacgatgcc agtatttggc aatcaagacg
     1741 tttagatgtc taaataaaac aataaggaca acacaacatg cctcacaatc ctatccgcgt
     1801 ggtcgtcggc ccggctaact acttttcaca tccaggaagt ttcaatcacc tgcacaattt
     1861 tttcactgat gaacaacttt ctcgcgcggt gtggatctac ggcaaacgcg ccattgctgc
     1921 ggcgcaaacc aaacttccgc cagcgtttgg acttccaggg gcaaagcata ttttgtttcg
     1981 tggtcattgc agcgaaagcg atgtacagca actggcggct gagtccggtg acgatcgcag
     2041 cgtggtgatt ggcgtcggcg gcggcgcact gctcgacacc gcgaaagccc tcgcccgccg
     2101 tctcggtctg ccatttgttg ccgctccgac gatcgccgcc acctgcgcag cctggacacc
     2161 gctctccgtc tggtataacg atgccggaca ggcgctgcat tatgagattt tcgacgacgc
     2221 caattttatg gtgctggtgg aaccggagat tatcctcaat gcaccgcaac aatatctgct
     2281 ggcggggatc ggtgacacgc tggcgaaatg gtatgaagcg gtggtgctgg ctccgcaacc
     2341 agaaacgttg ccgctaaccg tgcggctggg gatcaataat gcgcaggcca ttcgcgacgt
     2401 cttgttaaac agtagcgaac aggcacttgc cgatcagcaa aatcagcagt taacgcaatc
     2461 attttgcgat gtggtggatg ctattattgc tggtggtggg atggtcggtg gtctgggcga
     2521 tcgttttacg cgtgtggcgg cggctcatgc cgtgcataac ggtctgactg tcctgccgca
     2581 aaccgagaag tttcttcacg gcaccaaagt cgcttacgga attctggtgc aaagcgcctt
     2641 gctgggtcag gatgatgtgc tggcgcaatt aaccggggcg tatcagcgtt ttcatctgcc
     2701 gactacgctg gcggagctgg aggtggatat caataatcag gcggagatcg acaaagtgat
     2761 tgcccacacc ttgcgcccgg tggagtccat tcattacctg ccagtcacgc tgacaccaga
     2821 tacgttgcgt gcagcgttcg aaaaagtgga atcgtttaaa gcctgacgtg caaatttagc
     2881 agcagcgcgc gccgcctttt ccaccgtagc gcgcgtcctg ccgctcacgg aaaaactcct
     2941 cgtaggtcat cggcgtttga tcgggatgat taacccgcat atgttcgaca tagttgtcgt
     3001 aatcaggcat accaatcatc agcttcgccg cctgacctaa atattttccg gcttttgcca
     3061 gtgaatcaaa catcttggcc tccgctaacc atgttgctga aagagtgccg gatgcggcgt
     3121 gaacgcttta tccggcctac aaatgctatc aacgcactcg gtcagtcccc tcgccccatc
     3181 ggggagaggg ttagggtgag ggggaaccct cggcactatg ccaacattcg ccaacatccc
     3241 ccctcactct gactttagtg cgcgcctttc gcctgcgcta cgatctcctc gacattttcc
     3301 ggcatcggct catacggcgt ttctttcgcc gttggcttcg gatctttcaa tgccgcaagt
     3361 gccgtcttaa tcgagaacag tgccaggacc accacgacca ccataaagaa gatggttaac
     3421 ccggcatcca gacggttgtt gaacaccagt tgcgccagct gtgactcagt atactgcgac
     3481 ggaatattgc cgctgtcgat cattgcctgg aacttgttag caatgaccag gaagccgact
     3541 ttcgcatccg ggctaaacgc tttcagccag tcagcggtca gggtacaaat cagcagccag
     3601 gccgttggta ccagcgccac ccaggcgtaa cgttgacgtt tcatcttgaa caacaccacg
     3661 gcacagagca tcagcgccat ccctgccagc atctggttgg caataccaaa cagcggccac
     3721 agagtgttaa taccgcccag cggatcgacc acgctctgat ggaggaagta gccccacgcc
     3781 agtacgcaca gcgctgttgc cagcaggtta gcaggcagtg aatcggtccg cttcaggcca
     3841 ggagacacca cgcccagcag atcctgcaac ataaagcgcg cagcacgcgt acccgcatct
     3901 accgccgtca gaataaacag agcttcaaac agaatggcga agtgatacca gaacgccaca
     3961 tccatcatac cacccagcgc accgtgcagg atgtaagcca tccccaccgc caacgtcggc
     4021 gcaccgcccg cacgggaaat gatcgactgt tcacccactt cgctggcaat ctggttgagc
     4081 gtatctggtg taatgctaaa gccccagcta ctcaccacct gcgcggcaga agcgactaca
     4141 tccgccgtcc ctgccggagc cagcaccgcc attgggctgt tcatggcaaa atacacgccc
     4201 ggatcgatga tacaggcaga aactagcgcc ataatcgcca cgaaggattc catcaacatc
     4261 ccaccgtagc cgataaagca cgcctgccct tcgttcgcca gcatcttcgg cgtggtgccg
     4321 gaagagatca gcgcatggaa gccagacacc gcaccacagg cgatggtgat aaacaggaac
     4381 gggaacaggt taccggtcca taccgggcca gtgccatcga caaatttggt cagcgcaggc
     4441 atggtcagcg tcgggcgcat aatcaaaatg cctaccgcca gaccgacgat agtcccgatt
     4501 tt
//
LOCUS       DAZSNM010000257         4492 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZSNM010000257 DAZSNM010000000
VERSION     DAZSNM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.257"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            264..1178
                     /gene="sitA"
                     /locus_tag="ACHW42_003824"
     CDS             264..1178
                     /gene="sitA"
                     /locus_tag="ACHW42_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR8945439.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            1178..2005
                     /gene="sitB"
                     /locus_tag="ACHW42_003825"
     CDS             1178..2005
                     /gene="sitB"
                     /locus_tag="ACHW42_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR8945440.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            2002..2859
                     /gene="sitC"
                     /locus_tag="ACHW42_003826"
     CDS             2002..2859
                     /gene="sitC"
                     /locus_tag="ACHW42_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR8945441.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            2856..3713
                     /gene="sitD"
                     /locus_tag="ACHW42_003827"
     CDS             2856..3713
                     /gene="sitD"
                     /locus_tag="ACHW42_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR8945442.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(3914..>4492)
                     /locus_tag="ACHW42_003828"
     CDS             complement(3914..>4492)
                     /locus_tag="ACHW42_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR8945443.1"
                     /translation="REECTVEIKKIINPRYTESGAVDCDVFFDDRDQAVPYTATADDV
                     APTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAWRAEQEAKLFTFEWNG
                     RIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSMPELEELAAAMVQAQV
                     DRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
BASE COUNT         1049 a         1123 c         1105 g         1215 t
ORIGIN      
        1 atcagcgaca acaataagct acgataaccc actctttttt gccccatttt tgccatcaac
       61 aattttcctc ataaagtcat ttcaaagcct aatagctgct ctactttttt cctcaatatc
      121 atgtcgccag caggaaacaa cctaatccct gttttaggcc tcaaagctgt taatgataat
      181 tattttcatt ttgtataggt tatgctataa aacatagccg ttgctatgtt tgttctaatt
      241 ttgctcacta taggtactaa attatgcact caataaaaaa agtaaccatg ctcttggggt
      301 gcctcgccct cacctgctcg atcgcatttc aggcaagtgc aactgaaaaa ttcaaggtca
      361 ttacaacatt taccatcatc gcggatatgg ccaaaaacgt ggctggagat gctgcagaag
      421 tctcatccat aaccaagcct ggtgcagaaa ttcatgagta tcagcctacc cctggcgata
      481 ttaaacgtgc gcagggggca caactgattc tcgccaatgg tatgaatctg gaattgtggt
      541 tccaacgctt ttaccagcat ctcaatgggg ttccagaagt aattgtctct tcgggtgtga
      601 cgccagtagg gatcaccgaa ggaccctatg agggcaaacc taatccccat gcatggatgt
      661 cgccagataa tgctctgatt tacgtcgata atattcgtga tgcgttgata aaatacgatc
      721 cggcaaatgc acaaacctac caacgtaatg ccgatactta taaagccaag attacccaaa
      781 cccttgcccc cctgcgtaag cagattacgg aactccctga gaatcagcga tggatggtca
      841 ccagtgaagg ggctttttct tatctcgcac gcgatttggg gctaaaagag ctttatctgt
      901 ggccgattaa tgccgatcaa caaggaacac cgcagcaggt acgtaaggtt gttgatatag
      961 ttaagaaaaa tcatatcccg gcagtcttta gcgagagtac gatttccgat aaaccagcgc
     1021 gtcaggttgc gcgtgaaacc ggcgcacact acggcggtgt gctctatgtc gattccctga
     1081 gcacagaaaa cggcccggta cctacgtata tcgaccttct taaggttact accagtacgc
     1141 tggtacaggg aattaaagcc gggaaaaggg agaaataatg atgcaatctg caggcattgt
     1201 cgttaatgat gttactgtca cctggcgtaa cgggcacaca gcactgcgcg atgcatcctt
     1261 cacagtacca agtggatcta ttgccgctct ggttggggta aacggttccg gaaaatcgac
     1321 gttgtttaaa gcgattatgg gatttgtgcg tctgaccagc ggaaagatat ctgttctggg
     1381 tattcccacg cgacaggcgc tacagaaaaa cctggttgcc tacgtccctc agtcagaaga
     1441 ggttgactgg tcatttcctg tactggtgga agatgtggtg atgatgggcc gctatgggca
     1501 tatgggtatg ttgcgtatag ccaaaaagag ggatcgtcag attgtcaccg atgcgctgga
     1561 acgtgtcgat atggtggatt ttcgccatcg tcaaatcggc gagctttccg gtggacaaaa
     1621 gaaacgcgtc tttctggcga gagcaattgc acagcagggt gatgtgatcc tgctggatga
     1681 accttttacc ggcgttgatg tgaaaaccga agcgaaaatc atcagcctgt tgagggagtt
     1741 acgcgcggaa ggcaaaacga tgcttgtctc aacgcacaat ctcggctcag tcacgacatt
     1801 ttgcgattat acggtcatgg tcaaaggcac cgtcctggca agcgggccga cagacaccac
     1861 ttttacagcc gagaacctgg agctggcttt tagcggcgta ctgcgccatg tcacacttaa
     1921 cggctcggaa gaaagcatta ttactgacga tgaacgccct tttgtggcac atcgaccgtc
     1981 agcggtgcag agggaagcaa gatgaacgtg ctactggaac ccttcagcta tgaatatatg
     2041 ctcaatgcga tgtgggtctc ggcaatggtc ggcggtctct gcgctttcct gtcatgctat
     2101 ctgatgctaa aaggctggtc gctgattggc gatgcgctgt cgcactctat tgtcccgggt
     2161 gttgcggggg catatatgct ggggcttccg ttttctctgg gagccttctt ttccggcgga
     2221 ctggcagcgg gcagcatgct gtttcttaac caacgcaccc gcttaaaaga agatgccatc
     2281 atcggcctga tcttctcgtc cttttttggc ctggggctgt ttatggtgtc attgaacccg
     2341 acatccgtga acattcagac catcgtcctg ggcaatattc tggcaatcga tccggcggat
     2401 atcctgcaac tcactatcat tggcattctc tcgatcatcg tgcttttttt caaatggaaa
     2461 gatcttatgg tgaccttttt cgacgagaat cacgcccgtg ctattggatt acatccgggc
     2521 agattaaaaa tcctcttctt caccctgctg tcggtttcga ccgttgcggc gctgcaaacg
     2581 gttggtgcct ttctggtgat ctgtctggtg gtgactcccg gtgcaacagc atggttgtta
     2641 accgatcgtt ttccccgcct gctcataatt gctgtaacta ttggcagtgt aaccagtttc
     2701 ctcggcgcgt gggtcagtta ctttctggac ggcgcgacag gtggaatcat cgtggtggcg
     2761 cagacactgc tgtttctttt agcatttgta tttgcgccca cccatggact tttggcaaat
     2821 cgtcggcgtg cgcataaagc gctggaggat cgctcatgat ggcattatta ctggagccac
     2881 tgcagttcac ctttatgagt cacgcactgc tgatttcact ggtagtttcc ataccctgtg
     2941 cgctgctgtc ggtctttctg gtcctgaaag gttgggcact gatgggtgac gcgatgagtc
     3001 atgcggtttt tcctggcatc gtgcttacct ggattctggg gttacctctg gctacagggg
     3061 cattcgtcgc cggagtattc tgcgcggtgg cgacgggata cctgaaagac aacagccgaa
     3121 tcaagcaaga tacagttatg gggattgttt tttctggcat gtttgccgca ggtctgatct
     3181 tgtatattgc aattaaacca gacgtacatc ttgaccatat tcttttcggc gacatgctcg
     3241 ggataaccat cggcgacata atccagacgg tgattattgc cgggctggtt acacttgtta
     3301 ttagcgtaaa atggcgggat tttttgctgt tcagctttga ttatcaacag gcgcaggtaa
     3361 gcggtttgca tacgagatgg ctgcattatg ggctgctgtg catggtttcc ctgaccattg
     3421 tggcgacgct gaaagcagta ggtatcattc tctctatttc tctgctcatt gcgcctggcg
     3481 ccattgcagt actcctgaca caacgctttc atattgcgct cctactggcg actggcatat
     3541 cggtaatagt gtcaatgact ggtgtctggc tgtccttttt tatcgacagc gcaccagctc
     3601 cgacgattgt cgtcttattc gcggtcgtgt tcatcatgac gtttaccgtt accagcatca
     3661 acgcacgcac aaagggaaac gcccatacac aggatctgtt atcacccaat tagcacaaca
     3721 tccgaggaca gcatcgtccg ccagcctaca gaagtccgga aagccatgga aggtcgttgg
     3781 gaggaggtac gaattctatc atgcaaaaaa tacgtaaaat cgataacgcc tagaaatcat
     3841 tcaatactcg cactatcgaa agtttaccag ccaaccgcgg cacgttcctg catacgacgt
     3901 gccgcagctt ctgttattcc acgataaaat tcctaaccga attcaaatcc ttcagactat
     3961 tcagctcttc cttcagctcc cgctgacggc gataaatctc atcgttgcga tcgacctgcg
     4021 cctgcaccat tgctgccgcc agttcttcca gttccggcat cgacagtttc acctgctgat
     4081 tatcggcatc gctccacgcc atatgtgttt gtgctgtgac agattttgcc agcatgacta
     4141 ccggggacag gcggcccagt gagtcgggac cagcattcca gatacgaccg ttccattcaa
     4201 acgtgaacag cttcgcctcc tgttctgcgc gccatgcttc aatttcctga cgtctggcct
     4261 ctctggccgc ttccagcatt tctggtgtca cagtgaatgg ggctatctca ccccatttgc
     4321 cgctttgcag ttcctgccag atttgctgac ccgtcggagc gacatcatca gcggtggctg
     4381 tgtaggggac tgcctggtcc ctgtcgtcaa aaaaaacgtc acagtctact gcgccacttt
     4441 cggtataacg gggattaatg atttttttaa tttccacggt gcattcctca cg
//
LOCUS       DAZSNM010000258         4413 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZSNM010000258 DAZSNM010000000
VERSION     DAZSNM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.258"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..750
                     /gene="aspC"
                     /locus_tag="ACHW42_003829"
     CDS             <1..750
                     /gene="aspC"
                     /locus_tag="ACHW42_003829"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR8945444.1"
                     /translation="AYYDAENHTLDFDALINSLNEAQAGDVVLFHGCCHNPTGIDPTL
                     EQWQTLAQLSVEKGWLPLFDFAYQGFARGLEEDAEGLRAFAAMHKELIVASSYSKNFG
                     LYNERVGACTLVAADSEIVDRAFSQMKAAIRANYSNPPAHGASVVATILSSDALRAIW
                     EQELTDMRQRIQRMRQLFVNTLQEKGANRDFSFIIKQNGMFSFSGLTKEQVLRLREEF
                     GVYAVASGRVNVAGMTPDNMAPLCESIVAVL"
     gene            complement(973..1620)
                     /gene="gloC"
                     /locus_tag="ACHW42_003830"
     CDS             complement(973..1620)
                     /gene="gloC"
                     /locus_tag="ACHW42_003830"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR8945445.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(1647..2195)
                     /locus_tag="ACHW42_003831"
     CDS             complement(1647..2195)
                     /locus_tag="ACHW42_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR8945446.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(2376..4223)
                     /gene="ldtD"
                     /locus_tag="ACHW42_003832"
     CDS             complement(2376..4223)
                     /gene="ldtD"
                     /locus_tag="ACHW42_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR8945447.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
BASE COUNT         1058 a         1159 c         1098 g         1098 t
ORIGIN      
        1 gcttattatg atgcggaaaa tcacaccctt gacttcgatg cactgattaa cagtctgaac
       61 gaagctcagg ctggcgacgt agtgctgttc catggctgct gccataaccc aaccggtatc
      121 gaccctacgc tggaacaatg gcagacactg gcacaactct ccgttgagaa aggctggtta
      181 ccgctgtttg acttcgctta ccagggtttt gcccgtggtc tggaagaaga tgctgaagga
      241 ctgcgcgctt tcgcggctat gcataaagag ctgattgttg ccagttccta ctctaaaaac
      301 tttggcctgt acaacgagcg tgttggcgct tgtactctgg ttgctgccga cagtgaaatc
      361 gttgatcgcg cattcagcca aatgaaagcg gcgattcgcg ctaactactc taaccctcca
      421 gcacacggcg cttctgttgt tgccaccatc ctgagcagcg atgcattacg tgcgatttgg
      481 gaacaagagc tgactgatat gcgccagcgt attcagcgta tgcgtcagtt gttcgtcaat
      541 acgctgcagg aaaaaggtgc aaaccgcgac ttcagcttta tcatcaaaca gaacggcatg
      601 ttctccttca gtggtctgac aaaagaacaa gtgctgcgtc tgcgcgaaga gtttggcgta
      661 tatgctgttg cttctggtcg cgtaaatgtg gccgggatga caccagataa catggctccg
      721 ctgtgcgaat cgattgtggc agtgctgtaa gcgttaaaaa caatgaagcc cgctgaaaag
      781 cgggctgaga ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctacg
      841 cgtcccctgc aatattttga atttgcacga ttttgtaggc cggataaggc gctcgtgccg
      901 catccggcat aaacaaagcg cactttgcca gaaatccagg cccgcattaa aagtggactt
      961 ttttatgcgc ctttaccaga cgggcatttc gtcttgcagg aagggattgt gcaggcgctc
     1021 ataaccaagt gtagataatg gtccgtgacc cggaataaat gtcacgtcat cccccagtgg
     1081 cagcaattta tctttgattg aagaaatcag ttgattatga tcgccacgcg ggaagtcact
     1141 gcgccctact ccgcctttga aaataacatc gccagaaatc agcagctttg cccgatcatc
     1201 aaaaaacacg acatgacccg gcgtatgccc agggcaatgt aacacctgta aagtcacatt
     1261 ccctatgctg atggtatcgc cttcgttcag ccaacgatct ggcgtcagcg gctggcactc
     1321 ttccagacca aacatacgac tttgcgcagg caagccttgc agccagaact catcttcttt
     1381 ttccgggcca aaaaccggca cgccgtaatg ttgcgccagt tccgccgctg cgccaacgtg
     1441 gtccagatga ccatgcgtca gcaggatctg catcagtgtc aggccgctgt catcaacttc
     1501 ctgtttgatt ttttccgcat cgccgccagg atcgaccagt gcggccaggc gggtttgttc
     1561 acaccagatc aatgaacagt tctgggagaa tgcggtgacc ggaataatac gatagttcat
     1621 actgcccctg tttcgttaag cgattactac cagtgccgtg ctggcccggt atcaatatgc
     1681 acaaagttac tacgtgggta atatcctaca ccacctgcgc gcatagataa cgcggctttg
     1741 cgaatattgc ttaacgcgat accttcaata tggaaatcca tcgcctggcc tttagtgtga
     1801 tagcttttct tcgctactcc acggctgcgg gcgcgtagtt cattgttggt atcaatggaa
     1861 cgataaccgg aaatgagttg caccggtttg cgcgtgccta acaacccttg caggcgatac
     1921 aactggtcga ataatcctgg gtcgatggac tttattttgt tcgcgcggta atcgcggaaa
     1981 aaatggttaa gttttgccaa ttcttcctga atatagcctc tgccatcgaa aaactccgct
     2041 ttgattgact ctccggtatg aagattattg agtgtcaaaa tgcgcgggcg tggggtagag
     2101 agtgttgcaa acgcaggggt cggcaggatg gcggcaccga gtgcaacgcc accaagcgcc
     2161 agcaatttgc ggcgattagc gtcgaatttg tccatgataa tcaagtctac agctcaatgt
     2221 tatcgtttat atgcacttct ggcgcacaag aggcacctta accgcccgta aagcagacgt
     2281 caagatgact caaccccagc agtgacgggg gctgcagaga atcgccccca atcatgctaa
     2341 ttattacgac aattgatttc cccgaactac ttcatttacc tgattaattg ttccgctttc
     2401 gatacgattt gcgagctgga tcgcgcaggc agatcataat tgtaaatatc tgtacgatac
     2461 tgggtacgat catctgcacc aacaaaggcc gtcaggtagt agagattcac cggaatcgac
     2521 tgccgaatat tgacgtaacg tgtatcaccc tgcttcagcg catcagaaat acgtttgtca
     2581 ttccatcctg catcctgcaa cagcatattc gccagatcgg aagctttatt cactcgtaca
     2641 cagcctgagc tcaatgcgcg tgtatcacgc ttgaacagat tgtggttcgg cgtgtcatgc
     2701 aaataaatgg cctctgaact cggcatattg aatttatagc gccccagcga gttccgtggg
     2761 cctggagcct gctggaagcg aaacggtaaa ttcgaggccg tgattgtaga ccagtcaacc
     2821 tcccatgggt caatcgcttc tctgctgttc cagccgcgca tcaccgtata gccatggctt
     2881 tcgagatatc ccggatcgtt gcgcactttc ggcagaatat ctttgcgtgc cagagttggt
     2941 ggtacgttcc acggcgggtt taccactacg ttgttaagtg cactgctcat catcggcgtt
     3001 ttgcgatcgg ggcgaccgac aatgactcgc gaatccagca cctgattgcc gttctgatag
     3061 tagagcagcg aataggctgg aatgttaacc atgatcccgg tagaaagctc tgttggcagc
     3121 aagcgcaatc gctggatgtt gagtgccaac acgccagcac gctgggcggg cgttacgttt
     3181 aaccagtcac gcgttgccgg gccaatagca ccatctgctc ccaatccttg ccatgcctga
     3241 aaacgtttaa cggcttccac cagttcatta tcgtaggcgg cacgaacggc aggcgcaggt
     3301 ttactacgag acgtcgtttg cttgtccatc ggcttagttt ctgctgtttc aacagtaaca
     3361 gcggatgggc tgactaccgc gtcagttggc gtgtcatcgc caggtagagt aattttcggc
     3421 cccccgtcca acatgcctgt gcgttgcaat atttcgcgca acgccggtac gtcgttactc
     3481 cactgccctg ggcgcaacgt tgctttgccg gtcagttggg gccacggttt ggtgtcactg
     3541 agtaagacca gtaacgattc atgcatcgcc gcatattgcg gatgctgcgg tgccagtcct
     3601 gcaacaaacg taggcaattg acctttatcc agcgctagct gccattggtt aatcaccgaa
     3661 agcggcggcg ttgaaagcgc ataaggttta ctgctatata gccagcgagt gcctttgatc
     3721 ggaatatttg caatgaaatg gagatagccc atcatcgcat cagagagcac cacgtcacgt
     3781 gccatcccgt taacaccagg atcggtcagt aactctaccc atttattaaa ctgcggctgg
     3841 aaaccagcaa tcgccacctc tgccagctgt tgctggaagg ctttaacagc atcacggttt
     3901 tcccacatgg gttgcatatc gcgtgcggca tacaacagtt gaagctggtt aagataaacc
     3961 ggcttgtaac ctgcgggtaa ttgagattcg atttgcgtgc gggctttttc tgccgcacct
     4021 tcaggcaatg gctggatccc cgccattatt gccgtggctt gcgcctgcgg cagtgcctcg
     4081 ccctgttcac tgacagccac cgggctgtcg ccagggatta cttcaggctc atcggcctgc
     4141 gcattgaaca gtggagcgaa tgttacggcc aggcacaaac tgattgccga cagccgacga
     4201 ccacacatca tattaagcaa catcccttgc cccctgtttt tatcatactc tgaatagcca
     4261 ggctgttatt ttacgacttg cctgtacaac gccaatactc acgaaagtat gacgagcaag
     4321 cctgttactc atcaggctta tcttcagtat ataaacgccg atacgttttt acataaccta
     4381 atgtaaagaa gtttaaagaa aagcgcgccg tta
//
LOCUS       DAZSNM010000259         4288 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZSNM010000259 DAZSNM010000000
VERSION     DAZSNM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.259"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            365..568
                     /locus_tag="ACHW42_003833"
                     /pseudo
     CDS             365..568
                     /locus_tag="ACHW42_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            587..3973
                     /locus_tag="ACHW42_003834"
     CDS             587..3973
                     /locus_tag="ACHW42_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR8945448.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            3976..>4288
                     /locus_tag="ACHW42_003835"
     CDS             3976..>4288
                     /locus_tag="ACHW42_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945449.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TR"
BASE COUNT         1092 a          961 c         1304 g          931 t
ORIGIN      
        1 aaaagcttat agatttatag ttgataatta aaacgcatcc gatatttcag ctttttctat
       61 gcaagataca attttataag gtgtccattt ttatatggag gcggtagcgt gtctgttact
      121 agtggtttta tttgcatagt gaatatattg ggattgttaa atacataact atgtatccgc
      181 tctggtcgtt gtaaatattg ctaattgctt aatattatta gtgattatga ctggaaagat
      241 aaagtgtgct gtaaagcggt gatgggtatt acattcatga ttaaaaaaac agaacctgtc
      301 aataaataat tcctttatta aaacggacta tttacattat gacggaatat tatctcaacg
      361 aggcggtttg gaaatacaga gccagtttct ccggggtaac gagcaggtgt accagtgtca
      421 ggtggcattt gtattgccgg gtgagcgggt catgatggcc ttcacctacg ccagaaccac
      481 accgctgacc ccggcagata tgacgcgctg ggctgaaatt aaaaagaatc tgcgcttcag
      541 gatgcggcag gaagtcagga cgaattaatc atacagggaa cgggacatgg aagaggcatt
      601 ctgggcggca agacagggtg atgccctgct gcatacgtcg tttatggcag atgtgctggg
      661 ggcggtgctg gaggtggccg caaatgtcgt tattgatgcc ctcattgtcg gcacaacctc
      721 gttactggcg acgtcagtgg ggatcacgat ggggtgcacg gccgtcgtgc tgaccggttt
      781 tgtggcgggt gtggcgatgg tgtacaccgg cgtgacagaa aaggtcagcg aagcctgttc
      841 agccctcgca aatatgcttt tcccgcctca gattgaaggt tacatcctca ccggctctgg
      901 tgacacatgg atcaacagca aacctgccgc gcgggcagcg gcgactgcgg catcgcggca
      961 ggacatagag gcgcaggaag cgcaggcgaa ggcagaacag gaagaagcgc agcgtcagga
     1021 ggatgccaga acgttccggg atgtggccgg ggaatatctg gaaatggcgg gggccatagc
     1081 cctggcggtt aacccggtga cagggcctgt gctgatgtcc cgggctctgc acgaccagct
     1141 cagtacggaa gaagggcgtt ctgaaatggt ggatggcgtg acacattttg tgtcggaact
     1201 atggcagccc actgtggcca gtgcggcccc gggatccacc ccgacccctg acgataaaat
     1261 cgactgccat aaacatcctt cctcactgac gcagtttctc gcacaaaaaa aagccgcctt
     1321 tctggatgac cccctgggaa cggcccttaa cctgctgaat ccgggcggta tgctggaaac
     1381 cgcctttcag ggagtgaatg cggttattgg cagcatcagt aatctgttca aaggcgatga
     1441 cgagccgccc gcagcggaat atatcgcgga aggcgcgcgg gatgtgcgta tcaacagcca
     1501 gccggcgaca cgcagcgggg cacgctgtac ctgcgaggcc agggtggtga atgagccggg
     1561 gaacggggcg tttgtgtcac cggatgtgcg cattggcggt ccgttactgg tggtcaggga
     1621 tatcagaagc ggcaggtcgc agataaccct ggtggcaacc gtggcgctga tgtttcttcg
     1681 cccgggcaaa atgctttcta aaatcgcctg ttttgctgcc agtgtcggga tgagcatgct
     1741 gacgcagaag gccaccagtg cccttccgca tccggtcaat gccgccaccg gggccaaata
     1801 cctggccgat gacgatgact tcgatttcag tctgccgggc cactttccgc tggactggca
     1861 gcgcgtgtac agcagtcgtg acgaacgtac ggaaggaatg tttggtcagg gctggagtgt
     1921 aatgtatgaa gtcagcctcg tgtgcacacc gggtagcgca gacgaaaact gcatgacctt
     1981 tgtgtcgggc atggggcgac gcttggatat ggaagccgta ctgccggggg gcgggtttta
     2041 cagccccggt gaagggctgg cggtaaggcg tggtgagcag gggcactggc ttatcagcag
     2101 cgatgacggg cagtgttttc tgtttgaggc tgacccgcac cacccgcagc gccagcggct
     2161 gaaaatgctg ggcgaccgta acagcaactg tctgaatctg tactacgacg accggggccg
     2221 cattgtggaa atcagcggcg aacagcagcg tccgtgcatc cgtctgtatt acgaactggc
     2281 ggcgcatccc cggcgggtga cacagattta tcagcatttc cctgagactg cacctttact
     2341 gctgcgtcgt tacagctatg acgaggccgg ccacctgaac ggcgtgtacg acagcacggg
     2401 gcacctgctg cgtgagtttg cttacgacga aaaccactgc atgacgctgc accgccagcc
     2461 gggcggcgaa gggtattatt accagtgggg ctggtatgaa ggtccggatg acgcggcatg
     2521 gcgggtgacg gggcaccaca cggacagcgg tgaacagtac cggctggcgt gggacctggc
     2581 gcagcgcagg ctgtgcgtga cggacggact ggggcgcaca cgttatcacc agtgggatgc
     2641 gcagaatcag gtgacggcgt accaggacga agccggacag gtgacgacat tccgctggag
     2701 cgacgaagaa cgactgttgc tggggatgac cgaccctcag ggcggcaaat ggcgctatgt
     2761 gtatgacctg caggggcata tcacggagac gcacgacccg ctggggcggg tggagcaggc
     2821 gcagtggcac ccggtgtggc accagccgga aacggaggtg gacgcggcgg gaaacctgct
     2881 ggggaggcgc gcgggggaaa gggccacggc agaaaacagc gtggtgccgt tcaaccggct
     2941 gatgtcttac cgtggtgtgc actaccgtta tgacgaattt ggccgtgcgg tggaaaagga
     3001 gggccggagc ggaacgcaga gttaccgtta tgatgcggaa caccggatgg tggaagtgac
     3061 gacagcgcgg gggacatacc ggtatgtgta cgatgcactg gggaggcgga cggaaaaaca
     3121 gcacatcagc cctgacggta agccgtacaa ccggacaaaa tttttatggg acggaatgcg
     3181 gctggcgcag gagagcaggc cggagggaac aggcagcctg tacatctaca gggaccaggg
     3241 gagttatgaa ccgctggcgc gggtggataa ggcgggcaaa gagggcccga accggatact
     3301 gtattttcac acggatgtga acggcgcgcc ggaagagatg acggacagcg acgggaaaat
     3361 cgtgtgggag acggggtatc aggtctgggg taacacgata caggagaagg accacggcgg
     3421 ggtggagcag aacctgcgct accagggaca gtacctcgac agggaaacgg ggctgcacta
     3481 caatctgcac aggtactatg atccggatgt cgggcggttc atggtaactg acccgatagg
     3541 gttgagaggc ggtttaaatc tctacagtta tgcacctaat cctctaaaat atgctgatcc
     3601 acttggatta actccatgtg ctgttagtaa ccaaaaagcg aatcgtttat tagatagtag
     3661 tgaaaccaaa gttactgtta ggtctagaag tgacgctgaa caattgttta tggatagata
     3721 tttaggtcat aattataaaa atatgacagg tgagtcaggc cccagcacta agaacttaat
     3781 ggaatatctt actgaaaata aaacaaaagc aggttcatat cattgggatg atataaaaga
     3841 tccttctgta acaaaacctt catatagagt gtcaggtcat gggccaggaa atcctgatgg
     3901 tgatttacca catttacaag ttcatcaaca tggtggaagt gttagacata tatttttccc
     3961 gtgggagaca taataatgaa caatctcaat acattattaa gtggaaaggt tttgctcaaa
     4021 gaaattagtc cattaatgcc tggatataga acatggattg aaatttcatt gatcgatgaa
     4081 ttaaaaccat cttatccttt tagattagat gaatatgcta tgatagggca ttcaccttat
     4141 gccaatgaat gtaataaaga tgaagctaaa tttaaactac gaatatcatc atttttagct
     4201 tctgacatag ataatgaata cgatccatca tatgattatg ttggaaaata tgaaattatc
     4261 gcttcactta atgagttaaa aactaggt
//
LOCUS       DAZSNM010000260         4216 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZSNM010000260 DAZSNM010000000
VERSION     DAZSNM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.260"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(2..1042)
                     /locus_tag="ACHW42_003836"
     CDS             complement(2..1042)
                     /locus_tag="ACHW42_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR8945450.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(1115..1750)
                     /locus_tag="ACHW42_003837"
     CDS             complement(1115..1750)
                     /locus_tag="ACHW42_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR8945451.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            1878..2396
                     /gene="yhbO"
                     /locus_tag="ACHW42_003838"
     CDS             1878..2396
                     /gene="yhbO"
                     /locus_tag="ACHW42_003838"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR8945452.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(2376..2819)
                     /locus_tag="ACHW42_003839"
     CDS             complement(2376..2819)
                     /locus_tag="ACHW42_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR8945453.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            2870..3172
                     /gene="yhbQ"
                     /locus_tag="ACHW42_003840"
     CDS             2870..3172
                     /gene="yhbQ"
                     /locus_tag="ACHW42_003840"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR8945454.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(3159..3662)
                     /locus_tag="ACHW42_003841"
     CDS             complement(3159..3662)
                     /locus_tag="ACHW42_003841"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8945455.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(3656..4180)
                     /locus_tag="ACHW42_003842"
     CDS             complement(3656..4180)
                     /locus_tag="ACHW42_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR8945456.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
BASE COUNT         1042 a         1257 c         1008 g          909 t
ORIGIN      
        1 atcagaacaa cagcgccagc ccgccgacaa tcacaccaga cactgccacc atcgcccctg
       61 tcagccataa ggctttcgcc gggaacgctt tgcgcagcat aatcagtgac ggcaaactca
      121 ctgccgggag cgtcatcaac aatgccagcg ccggagcggt tcccatacct gccagcatca
      181 tcgtttgtac aatcggaatt tctgctgccg tgggaatgac aaacaagcat cctgctaccg
      241 ccatcgccac cacccacatc aggctgttat cgacagcacc atcggcatgg gggaataacc
      301 agacgcgtgc ggcacccaac accagtactg caaggatgta aaccgggatc gtactccaga
      361 aaagtgtcca tagcgccctg ccccagcggc taaaaaaacc gctctgtgct tccggtatgt
      421 caatttcgac cggtgcctgc gtttgcggtg tttcacgcac ccatttttgc accagcgtcg
      481 caatcagcaa caccatcacc agcccggcca ccagacgaat cgccgcaaaa ccccagccga
      541 ggacaaaccc cataaacacc agcgtcgccg ggtttaacac cggattgccc atccagaatg
      601 ccagcgcacc gcccatcgat acctgttggc gacgcattcc cgccgcgacc ggagccgcac
      661 agcaggtaca catcatgccc ggcagcgaaa acagcgttcc cagcagcgtg ccgcgaaagc
      721 gcgattgccc aagcgtacgc aacaaccagt cacgcgggat cagcacctga atcaacgaac
      781 cgaggatcac ccccagcacc gccgctttcc atactgcgag gaaatagatc atcgcgtaat
      841 ccaacgccgc ttgccacgga ttagcatccg cctgcgcaag gatagattta ccgatactgt
      901 gggtttcggc tgcagtaaac gctttgccgt agtaaggttg ccatttcaca taccagagac
      961 cggcaatgac aacgagaaag aaaagcgcgg gtttccacca ctgaatgggc gttgccgcct
     1021 gagatgaaga ctgaccagtc atagcattcc ccaggaataa gttatgataa ttgagcgcgt
     1081 gaatattacg ctcactatca attcttggga ataattattc agctcttttg cgtaattctg
     1141 aagagcttaa aatcgtcacg ccttcatgct caggccgcat cgcctctgcc agcatgaccc
     1201 gcgccacgtc tctcgcatca atggatttcc agttacctgg taacaaacgg aacagcggcg
     1261 caaaaagcgt ttcgttcatc cgctgtttgc tacgatcgcc cagtaacatc gacgggcgag
     1321 caatggtcaa tttcggccag ttctgggcaa ttaatgcttc ttccatctcc cctttgacgc
     1381 ggttatagaa aaacggcgag tgggcattgg cacccatagc actgaccacc agcatatgct
     1441 gcgcgcccag tcgccgcccg gttaatgcgg tatccactac cagcgtgtaa tcggcatgaa
     1501 taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc gagacaacaa aacacaatgt
     1561 cgatgggttc ggtgacctgc gccaacgcgt cactcagttg cggatcatgg ggattaaaca
     1621 ccccgggcat atcgcccaac ggacgtcgcg tcggcgcagc aatggcgtta actttcggtt
     1681 cattaatcaa catccgcagc aggtgaccgc ccaccagccc cgttgcgcct gtaatcagta
     1741 cctgactcat cttcgttcct ttacagaatt gtctgccttg cgctccccgg ctcatgcacc
     1801 agtcttaata gaccatgagg taattatccc cgattgtgga aaattcgcct catccaatgc
     1861 aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt aatcactgat gaatttgaag
     1921 attcagaatt tacttcaccc gcagacgagt tccgtaaagc cggacacgaa gtgattacta
     1981 ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaaagg agaagccagc gtgaccatcg
     2041 ataaatccat cgatgaagtg acgccagcgg agtttgatgc cctgttgcta ccgggtggtc
     2101 attcaccgga ttatctgcgt ggcgacaacc gttttgtcac ctttacccgt gattttgtga
     2161 atagcggcaa acctgttttc gccatctgtc acggtccgca gctgctgatc agcgccgatg
     2221 tgattcgggg gcgcaaactg accgcagtta aaccgatcat tattgatgtt aaaaatgcgg
     2281 gcgtggaatt ttacgatcag gaagtcgtgg ttgataaaga tcagctggtt accagccgga
     2341 caccggacga tctgccagcg tttaaccgcg aggcgttacg cctgctcggt gcctgagtcg
     2401 cgtagccaaa tcattttttt accaaagccc agcgtgttgt cggtgaattt gatttcatcg
     2461 agccggattt cccacaccgg ggccgacagc attctggcaa ccggaaagcg acgattgtac
     2521 gccttgcgcg cgaggtcgct ttcctcacct tccagcctgc ggatctcacc tttaaactgc
     2581 acaccgcgaa ttaacgctac cgttttcggc tgaccgttta ccgttccggc aaccgcagcc
     2641 tgcggcccac tcatctgcgc gtggcgcgtt ttttcttccg tcaaaatgta gaaggcgact
     2701 ttctgcgcat caaaaagata aaaggcatta gcgcaccaga gttccccttc ctgctgcaca
     2761 caccaggtga caacatgttg ttttgccagc caacggctga tggcgatgag tgtttccatt
     2821 gctgttctct tttatactgt gggcctgacc ttaacatgcg atgcagacga tgacaccctg
     2881 gtttctttac ttgatccgta ccgccgacaa taagctttat accgggatca ccacggatgt
     2941 cgaacgccgc tatcagcagc accaaagcgg caaaggggcg aaagcactgc gcgggaaagg
     3001 agaactgacg ctggcgtttt ctgcgccagt gggcgaccgt tcgctggcac tacgggcgga
     3061 atatcgcgtt aagcaactga ccaaacggca aaaagagcgt ctggtagcgg aaggcgcagg
     3121 gtttgcagaa ctgttgagca gcctgcaaac cccggagatt aaaagcgatt gaaatgctcg
     3181 tgatactcaa ccagaccggt tacgccattc agcgcgtcat ccgctaaacg atgtaccagg
     3241 aaggcgcttt cagtgcccgg ccagcggcaa cgcagatcgt gatgcgccgc cagttcaaag
     3301 ccgaaacgac tgtacagcgc cggatcgccc agcgtcacca ctgcggcata gccgaactca
     3361 ttaagcgaat cgagtccttc atagaccagt tggcgtgcca gcccctgtcc acggtatttt
     3421 tcatcaaccg ccaacggtgc catgccgacc cattgcaggt cttcgccctg cacatcaacc
     3481 ggactaaatg ccacatagcc aatgacctga ccttcgtcat ctgtcgccac cagccccagc
     3541 gtcagaaagc catcttcacg cagatcgtga accagcttcg cttccgcatc actttcgaat
     3601 gagcgacgca gcagggcatc aatacccggc gcatcaatgg gaatttctac tcgaattagc
     3661 atggttcacc taccgatgtc tgtttggttt caggcgcggt tttcattccc gcctcaacaa
     3721 aatccgccag ttgcagcagc atcatgcgta aggctttcgg catttgctcc agttcaatgg
     3781 cgtccatcag gtttttcaca tacaccccca gctccgtatc gccttcaatc accagccgac
     3841 gctggaagaa gagcgtatcc ggatcttgtt tacgcgccgc aatcatcagc agatcgctgg
     3901 cgtcggcact aaaactcaca tcagcttgcg tgttctggct gaccaccagt ttgccattca
     3961 ccaccgaggt aaaccattgc aggtcaatat cacgcacatg aatacttaac cagcggcctt
     4021 caagaaactc cagctcgcca tcatccagcg cctgacggaa ttgccagctt aagacctgct
     4081 caagaacctg gcgttttagc gcaaatggcg tcagttttac cggtacactc aacagagatg
     4141 gccccaaatg cacaatacgg gaacgcagtt tatccaacac gagttttact ccctgtttca
     4201 acaatcatcc tatttt
//
LOCUS       DAZSNM010000261         4209 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZSNM010000261 DAZSNM010000000
VERSION     DAZSNM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.261"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..209)
                     /locus_tag="ACHW42_003843"
     CDS             complement(<1..209)
                     /locus_tag="ACHW42_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR8945457.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHW42_003844"
     tRNA            complement(324..399)
                     /locus_tag="ACHW42_003844"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHW42_003845"
     tRNA            complement(406..480)
                     /locus_tag="ACHW42_003845"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHW42_003846"
     tRNA            complement(597..681)
                     /locus_tag="ACHW42_003846"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHW42_003847"
     tRNA            complement(690..765)
                     /locus_tag="ACHW42_003847"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHW42_003848"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHW42_003848"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR8945458.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHW42_003849"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHW42_003849"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR8945459.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHW42_003850"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHW42_003850"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR8945460.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAZSNM010000262         4198 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZSNM010000262 DAZSNM010000000
VERSION     DAZSNM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.262"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            84..752
                     /gene="ykgH"
                     /locus_tag="ACHW42_003851"
     CDS             84..752
                     /gene="ykgH"
                     /locus_tag="ACHW42_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR8945461.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="ACHW42_003852"
     CDS             complement(995..1690)
                     /locus_tag="ACHW42_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR8945462.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="ACHW42_003853"
     CDS             complement(1683..3110)
                     /locus_tag="ACHW42_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR8945463.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="ACHW42_003854"
     CDS             complement(3121..3840)
                     /locus_tag="ACHW42_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR8945464.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DAZSNM010000263         4185 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZSNM010000263 DAZSNM010000000
VERSION     DAZSNM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.263"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..192
                     /locus_tag="ACHW42_003855"
     CDS             <1..192
                     /locus_tag="ACHW42_003855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945465.1"
                     /translation="SIQSNRDWLSMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGIT
                     NIISIILQQAQDYDVAKIT"
     gene            complement(350..997)
                     /gene="ychE"
                     /locus_tag="ACHW42_003856"
     CDS             complement(350..997)
                     /gene="ychE"
                     /locus_tag="ACHW42_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR8945466.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(1028..1081)
                     /gene="ychE"
                     /locus_tag="ACHW42_003857"
     CDS             complement(1028..1081)
                     /gene="ychE"
                     /locus_tag="ACHW42_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFR8945467.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1474..4149
                     /gene="adhE"
                     /locus_tag="ACHW42_003858"
                     /gene_synonym="adhC"
     CDS             1474..4149
                     /gene="adhE"
                     /locus_tag="ACHW42_003858"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR8945468.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1168 a         1022 c          960 g         1035 t
ORIGIN      
        1 tcaattcaat caaatagaga ttggctaagc atgtctatct tttctgatta cagttccagt
       61 agtgaaatgc acaataattt aacaattgat tattatttag cactttcctc taccaaaggc
      121 agtggaatta ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg
      181 aaaataacat gatggtgagg agaagcagtt aaggaaatcc ttataactaa ctgtaatttg
      241 agaagaatat ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa
      301 taatgacgaa gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca
      361 ggccggggaa aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca
      421 atagccccat aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc
      481 acggtgccat gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga
      541 aaccaaacag atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg
      601 cccctggccc cgccatcaac ggcaacgcca atggcaccac accaatactt tcacgtaccg
      661 cggtttctga tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg
      721 acatcgctat cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac
      781 caaaaagttg tagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag
      841 acaggttggc tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc
      901 tgataaagac gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt
      961 tgaagtaaac gggaaaatca aaaagggtct gaatcacggt tcgctccgaa gcaaaagccg
     1021 gataatgtta gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca
     1081 tttttcacct cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa
     1141 aacatgctaa tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg
     1201 aaaagcgatg ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta
     1261 cccagaagtg agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc
     1321 taatgtttaa actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt
     1381 tcatcggtta agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt
     1441 actaaaaaag tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt
     1501 aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag
     1561 caagtagaca aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc
     1621 gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac
     1681 cactttgctt ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg
     1741 tctgaagacg acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt
     1801 atcgttccga ccactaaccc gacttcaact gctatcttca aatcgctgat cagcctgaag
     1861 acccgtaacg ccattatctt ctccccgcac ccgcgtgcaa aagatgcaac caacaaagcg
     1921 gctgatatcg ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg
     1981 atcgatcaac cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg
     2041 atcctcgcga ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct
     2101 atcggtgtag gcgcgggtaa cactccagtt gttattgatg aaactgctga tatcaaacgt
     2161 gcagttgcat ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa
     2221 cagtctgttg ttgttgttga ctctgtttat gacgcagtac gtgaacgttt cgcaacccac
     2281 ggcggctatc tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaat
     2341 ggtgcgctga acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc
     2401 ttctctgtac cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc
     2461 gaaccgttcg cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc
     2521 gaagacgcgg tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct
     2581 tgcctgtaca ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg
     2641 aaaacagctc gtatcctgat taacaccccg gcttctcagg gtggtatcgg tgacctgtat
     2701 aacttcaaac tcgcaccttc cctgactctg ggttgtggtt cctggggtgg taactccatc
     2761 tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa
     2821 aacatgttgt ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc
     2881 gcgctggatg aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc
     2941 ctgttcaaca atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa
     3001 actgaagtct tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca
     3061 gaactggcaa actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg
     3121 gacgccgcga agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg
     3181 gcgctgcgct ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa
     3241 gcgaaaatga tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg
     3301 gttgtaactg acgacgctac tggtcagaaa tacccgctgg cggactatgc gctgaccccg
     3361 gatatggcga ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc
     3421 ggtggtctgg acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag
     3481 ttctctgatg gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc
     3541 taccacgaag ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcaactatc
     3601 gcgggtatcg catttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg
     3661 ggttcccagt tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt
     3721 cgctacaatg cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg
     3781 caggctcgcc gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc accgggcgac
     3841 cgtactgctg ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg
     3901 ggtattccga aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg
     3961 gataaactgt ctgaagatgc attcgatgac cagtgcaccg gcgctaaccc gcgttacccg
     4021 ctgatctccg agctgaaaca gattctgctg gatacctact acggtcgtga ttatgtagaa
     4081 gatgaaactg cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa
     4141 tccgcttaat cagtagcgct gtctggcaat ataaacggcc ccttc
//
LOCUS       DAZSNM010000264         4182 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZSNM010000264 DAZSNM010000000
VERSION     DAZSNM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.264"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..186)
                     /locus_tag="ACHW42_003859"
     CDS             complement(<1..186)
                     /locus_tag="ACHW42_003859"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR8945469.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHW42_003860"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHW42_003860"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR8945470.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="ACHW42_003861"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="ACHW42_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR8945471.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="ACHW42_003862"
     CDS             3863..4144
                     /locus_tag="ACHW42_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR8945472.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1043 c         1062 g         1061 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagt
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAZSNM010000265         4168 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZSNM010000265 DAZSNM010000000
VERSION     DAZSNM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.265"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHW42_003863"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHW42_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR8945473.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACHW42_003864"
                     /pseudo
     CDS             complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACHW42_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACHW42_003865"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACHW42_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
     gene            3787..>4168
                     /locus_tag="ACHW42_003866"
     CDS             3787..>4168
                     /locus_tag="ACHW42_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFR8945474.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1039 a         1208 c         1020 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctacatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DAZSNM010000266         4161 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZSNM010000266 DAZSNM010000000
VERSION     DAZSNM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.266"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            91..1155
                     /gene="ulaG"
                     /locus_tag="ACHW42_003867"
     CDS             91..1155
                     /gene="ulaG"
                     /locus_tag="ACHW42_003867"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR8945475.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1263..2018
                     /gene="ulaR"
                     /locus_tag="ACHW42_003868"
     CDS             1263..2018
                     /gene="ulaR"
                     /locus_tag="ACHW42_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR8945476.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2015..2764)
                     /gene="yjfP"
                     /locus_tag="ACHW42_003869"
     CDS             complement(2015..2764)
                     /gene="yjfP"
                     /locus_tag="ACHW42_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR8945477.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2946..3275
                     /gene="bsmA"
                     /locus_tag="ACHW42_003870"
     CDS             2946..3275
                     /gene="bsmA"
                     /locus_tag="ACHW42_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR8945478.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3424..3699
                     /gene="yjfN"
                     /locus_tag="ACHW42_003871"
     CDS             3424..3699
                     /gene="yjfN"
                     /locus_tag="ACHW42_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR8945479.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3816..>4161)
                     /locus_tag="ACHW42_003872"
     CDS             complement(3816..>4161)
                     /locus_tag="ACHW42_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR8945480.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
BASE COUNT         1097 a         1039 c         1057 g          968 t
ORIGIN      
        1 ggtcattacc ttcctgaagg aatagtgtca ggattaatca aaaccaatca catgttgatt
       61 cgaattgaac tatcatgaag gtaaatggcg atgagtaaag tgaaaagtat cacccgagaa
      121 tcctggatcc tgagcacttt cccggagtgg ggtagctggc tgaatgaaga aattgaacaa
      181 gagcaggtcg cccctggcac atttgcgatg tggtggctag gctgcaccgg gatctggttg
      241 aaatcagaag gtggcaccaa cgtttgcgtt gatttctggt gcggcactgg caaacaaagt
      301 cacggtaacc cgttaatgaa acagggtcac cagatgcagc gcatggctgg cgtgaaaaaa
      361 ctgcagccaa acctacgcac caccccgttt gttcttgatc cgtttgcgat tcgccagatc
      421 gacgcggtac tggcgactca cgatcacaac gatcatatcg acgttaacgt cgctgctgcc
      481 gtgatgcaga actgtgctga tgacgtacca ttcattggac cgaaaacctg cgttgatttg
      541 tggattggct ggggcgtacc gaaagagcgt tgcatcgtgg tcaaaccggg cgatgttgta
      601 aaagtgaaag acattgaaat tcatgcgctt gacgctttcg accgtactgc actgatcacc
      661 ctgcctgccg atcaaaaagc ggctggcgta ctgccagatg gcatggacga tcgcgcagta
      721 aactacctgt tcaaaacccc tggcggttcc ctatatcaca gtggcgattc ccactactct
      781 aactattatg cgaagcacgg taacgaacat cagatcgatg tggcgttagg atcgtatggt
      841 gagaacccgc gcggtattac cgacaaaatg accagcgccg atatgctgcg tatgggtgaa
      901 gccctgaatg cgaaagtggt tatcccgttc caccacgaca tctggtcaaa cttccaggcc
      961 gatccgcaag agatccgcgt gctgtgggag atcaaaaaag atcgtctgaa gtatggcttc
     1021 aagccgttta tctggcaggt tggcggcaaa tttacctggc cgctggataa agacaacttc
     1081 gagtaccact atccgcgcgg tttcgatgat tgcttcacta ttgaaccgga tctgccgttc
     1141 aagtcattcc tgtaatctgt cgtttatacc ggatagcgtg gagaagttcg cgctatccgg
     1201 cgtagtattt cctggctatt tcaaatatca tctaaaaaaa tcaaatttta tcggaatagc
     1261 tcatgactga agcacaaaga catcaaatcc tcctggaaat gctcgcacaa ttgggctttg
     1321 tgaccgttga gaaagtcgtt gagcgtctgg gaatttcgcc tgccactgcg cgacgcgata
     1381 tcaataaact tggcgaaagc ggcaaactga aaaaggtgcg caatggcgca gaagctatta
     1441 cccaacagcg cccgcgctgg acgccgatga atctgcatca ggcgcagaat cacgatgaaa
     1501 aagtacgtat tgctaaagcg gcctcgcagc tggttaatcc gggcgaaagc gtagtcatca
     1561 actgcggctc caccgcgttt ctgcttgggc gggaaatgtg tggcaagcca gtgcaaatca
     1621 tcactaatta tctaccgctg gcaaattacc tgatcgatca agaacatgac agcgtgatca
     1681 ttatgggtgg acagtacaac aaaagtcagt ccatcacttt aagcccgcag ggcagcgaaa
     1741 acagtctcta tgccgggcac tggatgttta ccagcggaaa agggctgacc gcagaagggt
     1801 tgtataaaac cgatatgctg acagcaatgg cagagcagaa gatgctgagc gtggtaggga
     1861 aactggtggt actggttgat agcagtaaga ttggcgaacg cgcgggaatg ctttttagcc
     1921 gtgccgatca aatcgatatg ctgatcaccg gcaaaaatgc taacccggaa atcctgcaac
     1981 aactggaagc gcaaggggtc agcattctgc gtgtttaaag atgctggcgg aaaaatgtca
     2041 cggcagcatc taacgcctca ggcgtaatgc ggtgacgcac gcctggctgc catgaacagg
     2101 ttagcagttt atcccgtccc gtctcgctta aggcctgctg caaacgtagt gattcgtcgg
     2161 caggcacaac gtcatcgtcg aggccatgcc acagcagtag aggtctgtcc ccaagttgtt
     2221 ccaggtggtt tgtcgcttcc cactctgcca gcggcgcgac aattttattg aattcattct
     2281 gctgtgctgc cgtttcaggt atcagcggtg gaaacagtga acgggcgagc gatgtaaaat
     2341 agcccgatcc catcatgctg gcggtacatt tcaccgtggg gtggcgagcg gtaattccca
     2401 gtgccgtcat cgcgcccatc gacgcgccac cgactgccag acggtcatca agcagccagt
     2461 tttcttcggc tattgccgca cgtaaagtag tgaattcctg catactttgt agcaagattt
     2521 gccagaattg atttaaccgc cgcgcttcgt cgccactaaa acggctaccg tgatcgggcg
     2581 catccggcat gatcacccgc aaaccagcct gcgccagcgc aacggcaaaa tagctataca
     2641 ccaggctgga tgaagtaaag ccgtgataaa aaattacgca cggtaacggg gtatcttttt
     2701 gcccgacagg ataagcatga agaacgggaa tatctgccag ctcgcgtgat tctatttcaa
     2761 tcatctgtct ctcctttttg tctggtatga catgaagaaa tccttagcac aagtttctgc
     2821 tggcggtatt agttcataat gttgagatgt gggttacgct ttcgtcacgt tttcattcga
     2881 aaatcgcgta ccaggtaaca attcgggaac attcccccaa agttaaatta ataaggcact
     2941 acactatggt tagcaggaaa cgtaatagcg ttatttaccg gtttgccagt ttattattgg
     3001 tgttgatgtt aagtgcctgt agcgcactgc aaggtacgcc acagccagca ccgccagtaa
     3061 cggaccatcc gcaagagatt cgccgcgacc agacgcaagg gttacagcga ataggtagcg
     3121 taagcacgat ggttcggggt tctccggatg acgcattagc agaaatcaga gcaaaagctg
     3181 tcgctgcaaa agctgattat tacgttgtcg tcatggtgga cgaaaccatc gtgacaggac
     3241 agtggtattc acaagccatt ttgtaccgta aataaatcag acaacttaca tagtcagctt
     3301 tacattgctt tgcgtcccta tgcgtttacc tgtgcacaat aaattaccgg ctggaagctt
     3361 gggcgtttat taccgcgatg gaatgccctg caacatgtgg ggaaacgaaa aatggagctg
     3421 acgatgaaac aattacttgc ctcaccctcg ctgcaattag tgacttatcc tgcgagcgcc
     3481 acggcacagt ctgcagaatt cgccagtgct gactgcgtga cgggcctgaa tgaaataggt
     3541 cagatctcag ttagcaatat ttcaggggat ccgcaggacg tggaacgtat cgtggcgtta
     3601 aaggccgatg aacaaggtgc gtcatggtac cgcattatta ccatgtacga agaccagcag
     3661 cctgataact ggcgcgtaca ggcgatactg tatgcatgag tgtcatctac agcaaatgaa
     3721 caatgccgga tgcggcgcga gcgccttgtc cggcctacaa ttttcacata tttcatttag
     3781 ttaatcgaaa ccagcgtcgc atcagtcgta tacgcttaca cacacactcc ccccgtcgcc
     3841 cgcagcaata aatcattctg gatctgctct gacaaccgta cgccgccgcg cgtatctaac
     3901 atcacctgac accacgcctg cgccattggc ggagaagcat atttcaacat ttgcgcaccg
     3961 cagcccagca ggaatagctg atgagtaatc tctcgcccca gttcttcagc tggcttacgc
     4021 agctgctgct gtaaacgacg aaccgcgcga tcaaaatagc gatcctgccc tttcacttcc
     4081 acaaatgctt ccgacaataa gtcgtatacg cccgcttgct tattgagaac gcgcaacaca
     4141 tccaggcaca taatattgcc g
//
LOCUS       DAZSNM010000267         4114 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZSNM010000267 DAZSNM010000000
VERSION     DAZSNM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.267"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..158)
                     /locus_tag="ACHW42_003873"
     CDS             complement(<1..158)
                     /locus_tag="ACHW42_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR8945481.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="ACHW42_003874"
     CDS             complement(308..469)
                     /locus_tag="ACHW42_003874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945482.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(469..654)
                     /locus_tag="ACHW42_003875"
                     /pseudo
     CDS             complement(469..654)
                     /locus_tag="ACHW42_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            914..1249
                     /locus_tag="ACHW42_003876"
     CDS             914..1249
                     /locus_tag="ACHW42_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR8945483.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="ACHW42_003877"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="ACHW42_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="ACHW42_003878"
     tRNA            complement(2161..2237)
                     /locus_tag="ACHW42_003878"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="ACHW42_003879"
     tRNA            complement(2339..2414)
                     /locus_tag="ACHW42_003879"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="ACHW42_003880"
     CDS             complement(2559..3380)
                     /locus_tag="ACHW42_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR8945484.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="ACHW42_003881"
     CDS             complement(3395..3751)
                     /locus_tag="ACHW42_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR8945485.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4114)
                     /locus_tag="ACHW42_003882"
     CDS             complement(3764..>4114)
                     /locus_tag="ACHW42_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR8945486.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1155 a         1117 c          793 g         1049 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgta
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgg
//
LOCUS       DAZSNM010000268         4091 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZSNM010000268 DAZSNM010000000
VERSION     DAZSNM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.268"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACHW42_003883"
     CDS             complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACHW42_003883"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR8945487.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACHW42_003884"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACHW42_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR8945488.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="ACHW42_003885"
     CDS             complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="ACHW42_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR8945489.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
BASE COUNT         1092 a         1067 c          894 g         1038 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
     3001 tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt
     3061 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac
     3121 gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt
     3181 aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa
     3241 gaatacgcca ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttctt
     3301 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg
     3361 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
     3421 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
     3481 cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt
     3541 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
     3601 cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca
     3661 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
     3721 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
     3781 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
     3841 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
     3901 aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac
     3961 aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat
     4021 tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct cgcagttatt
     4081 aaccttgtgt a
//
LOCUS       DAZSNM010000269         4078 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZSNM010000269 DAZSNM010000000
VERSION     DAZSNM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.269"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1100)
                     /locus_tag="ACHW42_003886"
     CDS             complement(<1..1100)
                     /locus_tag="ACHW42_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR8945490.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="ACHW42_003887"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="ACHW42_003887"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR8945491.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="ACHW42_003888"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="ACHW42_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR8945492.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="ACHW42_003889"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="ACHW42_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR8945493.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="ACHW42_003890"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="ACHW42_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR8945494.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="ACHW42_003891"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="ACHW42_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR8945495.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..>4078
                     /locus_tag="ACHW42_003892"
     CDS             3404..>4078
                     /locus_tag="ACHW42_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFR8945496.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGN"
BASE COUNT         1019 a         1023 c          952 g         1084 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaataatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaaaaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataacttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaat
//
LOCUS       DAZSNM010000270         4042 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZSNM010000270 DAZSNM010000000
VERSION     DAZSNM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.270"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(126..284)
                     /locus_tag="ACHW42_003893"
     CDS             complement(126..284)
                     /locus_tag="ACHW42_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR8945497.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEI
                     LRVLEVVA"
     gene            complement(886..1077)
                     /locus_tag="ACHW42_003894"
     CDS             complement(886..1077)
                     /locus_tag="ACHW42_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR8945498.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="ACHW42_003895"
     CDS             1142..1465
                     /locus_tag="ACHW42_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR8945499.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1674..2105
                     /locus_tag="ACHW42_003896"
                     /pseudo
     CDS             1674..2105
                     /locus_tag="ACHW42_003896"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="ACHW42_003897"
     CDS             2143..2673
                     /locus_tag="ACHW42_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR8945500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="ACHW42_003898"
     CDS             2680..2913
                     /locus_tag="ACHW42_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR8945501.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="ACHW42_003899"
     CDS             complement(3459..3851)
                     /locus_tag="ACHW42_003899"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945502.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          917 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAZSNM010000271         4015 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZSNM010000271 DAZSNM010000000
VERSION     DAZSNM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.271"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            241..2157
                     /gene="dnaK"
                     /locus_tag="ACHW42_003900"
     CDS             241..2157
                     /gene="dnaK"
                     /locus_tag="ACHW42_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR8945503.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2246..3376
                     /gene="dnaJ"
                     /locus_tag="ACHW42_003901"
     CDS             2246..3376
                     /gene="dnaJ"
                     /locus_tag="ACHW42_003901"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR8945504.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3480..3632)
                     /gene="mokC"
                     /locus_tag="ACHW42_003902"
     CDS             complement(3480..3632)
                     /gene="mokC"
                     /locus_tag="ACHW42_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR8945505.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1074 a          990 c         1051 g          900 t
ORIGIN      
        1 atatgaataa aattgctgtc aattttacgt cttgtcctgc catatcgcga aatttctgcg
       61 caaaagcaca aaaaattttt gcatctcccc cttgatgacg tgggttacga ccccatttag
      121 tagtcaaccg cagtgagtga gtctgcaaaa aaatgaaatt gggcagttga aaccagacgt
      181 ttcgccccta ttacagactc acaaccacat gatgaccgaa tatatagtgg agacgtttag
      241 atgggtaaaa taattggtat cgacctgggt actaccaact cttgtgtagc gattatggat
      301 ggcaccattc ctcgcgtgct ggagaacgcc gaaggcgatc gcaccacgcc ttctatcatt
      361 gcctataccc aggatggtga aactctggtt ggtcagccgg ctaaacgtca ggcagtgacg
      421 aacccgcaaa acaccctgtt tgcgattaaa cgcctgattg gccgccgctt ccaggacgaa
      481 gaagtacagc gtgatgtttc catcatgccg ttcaaaatta ttgctgctga taacggcgac
      541 gcatgggtcg aagttaaagg ccagaaaatg gcaccgccgc agatttctgc tgaagtgctg
      601 aaaaaaatga agaaaaccgc tgaagattac ctgggtgaac cggtaactga agctgttatc
      661 accgtaccgg catactttaa cgatgctcag cgtcaggcaa ccaaagacgc aggccgtatc
      721 gctggtctgg aagtaaaacg tatcatcaac gaaccgaccg cagctgcgct ggcttacggt
      781 ctggacaaag gcactggcaa ccgtactatc gcggtttatg acctgggggg tggtactttc
      841 gatatttcta ttatcgaaat cgacgaagtt gacggcgaaa aaaccttcga agttctggca
      901 accaacggtg atacccacct gggtggtgaa gacttcgaca gccgtctgat caactatctg
      961 gttgaagaat tcaagaaaga tcagggcatt gacctgcgca acgatccgct ggcaatgcag
     1021 cgcctgaaag aagcggcaga aaaagcgaaa atcgaactgt cttccgctca gcagaccgac
     1081 gttaacctgc catacatcac tgcagacgcg accggtccga aacacatgaa catcaaagtg
     1141 actcgtgcga aactggaaag cctggttgaa gatctggtaa accgttccat tgagccgctg
     1201 aaagttgcac tgcaggatgc tggcctgtcc gtatctgata tcgacgacgt tatcctcgtt
     1261 ggtggtcaga ctcgtatgcc aatggttcag aagaaagttg ctgagttctt tggtaaagag
     1321 ccgcgtaaag acgttaaccc ggacgaagct gtagcaatcg gtgctgctgt tcagggtggt
     1381 gttctgactg gtgacgtaaa agacgtactg ctgctggacg ttaccccgct gtctctgggt
     1441 atcgaaacca tgggcggtgt gatgacgacg ctgatcgcga aaaacaccac tatcccgacc
     1501 aagcacagcc aggtgttctc taccgctgaa gacaaccagt ctgcggtaac catccatgtg
     1561 ctgcagggtg aacgtaaacg tgcggctgat aacaaatctc tgggtcagtt caacctagat
     1621 ggtatcaacc cggcaccgcg cggcatgccg cagatcgaag ttaccttcga tatcgatgct
     1681 gacggtatcc tgcacgtttc cgcgaaagat aaaaacagcg gtaaagagca gaagatcacc
     1741 atcaaggctt cttctggtct gaacgaagat gaaatccaga aaatggtacg cgacgcagaa
     1801 gctaacgccg aagctgaccg taagtttgaa gagctggtac agactcgcaa ccagggcgac
     1861 catctgctgc acagcacccg taagcaggtt gaagaagcag gcgacaaact gccggctgac
     1921 gacaaaactg ctatcgagtc tgcgctgact gcactggaaa ctgctctgaa aggtgaagac
     1981 aaagccgcta tcgaagcgaa aatgcaggaa ctggcacagg tttcccagaa actgatggaa
     2041 atcgcccagc agcaacatgc ccagcagcag actgccggtg ctgatgcttc tgcaaacaac
     2101 gcgaaagatg acgatgttgt cgacgctgaa tttgaagaag tcaaagacaa aaaataatcg
     2161 ccctataaac gggtaattat actgacacgg gcgaagggga atttcctctc cgcccgtgca
     2221 ttcatctagg ggcaatttaa aaaagatggc taagcaagat tattacgaga ttttaggcgt
     2281 ttccaaaaca gcggaagagc gtgaaatcag aaaggcctac aaacgcctgg ccatgaaata
     2341 ccacccggac cgtaaccagg gtgacaaaga ggccgaggcg aaatttaaag agatcaagga
     2401 agcttatgaa gttctgaccg actcgcaaaa acgtgcggca tacgatcagt atggtcatgc
     2461 tgcgtttgag caaggaggca tgggcggcgg tggttttggc ggcggcgcag acttcagcga
     2521 tatttttggt gacgttttcg gcgatatttt tggcggcgga cgtggtcgtc aacgtgcggc
     2581 gcgcggtgct gatttacgct ataacatgga gctcaccctc gaagaagctg tacgtggcgt
     2641 gaccaaagag atccgcattc cgactctgga agagtgtgac gtttgccacg gtagcggtgc
     2701 aaaaccaggt acacagccgc agacctgtcc gacctgtcat ggttctggtc aggtgcagat
     2761 gcgccaggga ttcttcgctg tacagcagac ctgtccacac tgtcagggcc gcggtacgct
     2821 gatcaaagat ccgtgcaaca aatgtcatgg tcatggtcgt gttgagcgca gcaaaacgct
     2881 gtccgttaaa atcccggcag gggtggacac tggagaccgc atccgtcttg cgggcgaagg
     2941 tgaagcgggc gagcatggcg caccggcagg cgatctgtac gttcaggttc aggttaaaca
     3001 gcacccgatt ttcgagcgtg aaggcaacaa cctgtattgc gaagtcccga tcaacttcgc
     3061 tatggcggcg ctgggtggcg aaatcgaagt accgaccctt gatggtcgcg tcaaactgaa
     3121 agtgcctggc gaaacccaga ccggtaagct attccgtatg cgcggtaaag gcgtcaagtc
     3181 tgtccgcggt ggcgcacagg gtgatttgct gtgccgcgtt gtcgtcgaaa caccggtagg
     3241 cctgaacgaa aagcagaaac agctgctgca agagctgcaa gaaagcttcg gtggcccaac
     3301 cggcgagcac aacagcccgc gctcaaagag cttctttgat ggtgtgaaga agttttttga
     3361 cgacctgacc cgctaacctc cccaaaagcc tgcccgtggg caggcctggg taaaaatagg
     3421 gtgcgttgaa gatatgcgag cacctgtaaa gtggcgggga tcactccccg ccgttgctct
     3481 tactcggatt cgtaagccgt gaaaacagca acctccgtct ggccagttcg gatgtgaacc
     3541 tcacagaggt cttttctcgt taccagcgcc gccactacgg cggtgataca gatgacgatc
     3601 agggcgacaa tcatcgcctt atgctgcttc attgctctct tctccttgac cttacggtca
     3661 gtaagaggca ctctacatgt gttcagcata taggaggcct cgggttgatg gtaaaatatc
     3721 actcggggct tttctctatc tgccgttcag ctaatgcctg agacagacag cctcaagcac
     3781 ccgccgctat tatatcgctc tctttaaccc attttgtttt atcgattcta atcctgaaga
     3841 cgcctcgcat ttttgtggcg taatttttta atgatttaat tatttaactt taatttatct
     3901 cttcatcgca attattgacg acaagctgga ttatttttga aatattggcc taacaagcat
     3961 cgccgactga caacaaatta attattactt ttcctaatta atccctcagg aatcc
//
LOCUS       DAZSNM010000272         3938 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZSNM010000272 DAZSNM010000000
VERSION     DAZSNM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.272"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..133
                     /locus_tag="ACHW42_003903"
     CDS             <1..133
                     /locus_tag="ACHW42_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR8945506.1"
                     /translation="LNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            288..623
                     /locus_tag="ACHW42_003904"
                     /pseudo
     CDS             288..623
                     /locus_tag="ACHW42_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            620..1075
                     /locus_tag="ACHW42_003905"
     CDS             620..1075
                     /locus_tag="ACHW42_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945507.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(1153..2064)
                     /gene="rdgC"
                     /locus_tag="ACHW42_003906"
     CDS             complement(1153..2064)
                     /gene="rdgC"
                     /locus_tag="ACHW42_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR8945508.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            2189..3097
                     /gene="mak"
                     /locus_tag="ACHW42_003907"
     CDS             2189..3097
                     /gene="mak"
                     /locus_tag="ACHW42_003907"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR8945509.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            complement(3240..>3938)
                     /locus_tag="ACHW42_003908"
     CDS             complement(3240..>3938)
                     /locus_tag="ACHW42_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8945510.1"
                     /translation="IAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFA
                     ATMFGNAGVFAWFSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSP
                     LRIAAVTDFIIVLALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGE
                     LLGAAGGQIAFNLGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKR
                     QQAADSPVLAKPLG"
BASE COUNT         1003 a          973 c         1004 g          958 t
ORIGIN      
        1 gctgaatgtg ttactgcctg acgcgaccga ctggcaagtg tatgaagccg gttcggtgtt
       61 taatgttccc ggtcacagtg agtttcatct tcaagttgcc gaacctacct cttatctgtg
      121 ccgctatctg taattcctcg ccttcccctt gaacgggagg gcatttttct gaaatatcct
      181 ctctttaacc cataataata tttcctttgc tgcgattttt tcaatttccg atatattcat
      241 aattcatcaa ggttgatata aatatcagtg aagatctcca ggtatttttt gcggaactgg
      301 ctacgataaa agataaatca gatgatgaat ggtggcgtgc attgctgcaa agtcgcaatg
      361 atcatttgcg ccaaacggca ttacgcaacg cacatacccc ggcgtcgttg ttaacgacat
      421 tgactgagcc tcaggatcgg tcgctggcta ttaataatcc acagctggct gccgatgtga
      481 aaacggcgtg gttaaaagag gatccatcat tactcttatt tgtcgaacaa cccgatcttt
      541 cgcagttacg tgatttagtg aaaaccgggg caacgcggaa aattcgcagt gaagcgcgtc
      601 accggcttga ggaaaaacaa tgacacaacg tccatggagc aagttacagc gggagatcta
      661 tgacttactg acgccaacga tcaacctgca aattcactgt acccgttatc ccatgcgcag
      721 ccaaaatggt ggaagtaccg atttaccgcg ttattggatc acgttggata aaaacgtgat
      781 ctgggattac ccgaaagact ttattgcagg taacgctgga gtgcgtaatt ttcacggtga
      841 aacttgctgg tatccttacc tgacggatat ttgttcaatt tctgatttat tgcgtgaata
      901 tattgatacc ccaaaagcgg agctgttaac aaaacaattt accagcgata aatgggggtt
      961 agtgaatatt ctgcgtgctg ctgacaggcg aatagggatg aggcgattag atcagttacg
     1021 gcgtaaaacg cataatattg ctgcgctaaa aattattgct cgccgtagcg aataattata
     1081 tgcctggtgt ggcttcgtac gccggataag acgcggcagg cgtcgcatcc ggcattaaag
     1141 gaaaatcagc aattaacgtt gtgcttcgcc acctaatcct tcaatcaggt tttgaattaa
     1201 cgctgccagt tcaccagtca tcaggatgaa atcggcatca aaacgctggg cgaaatcttc
     1261 acggtcgata tcttcgtttt gatcgcgcag ctcgtcgcag aacttaagac gcttgagcga
     1321 accatcgtcg cacatcacaa actgaatgcg ctgctgccag tcgagcgcca gtttagtcac
     1381 cacttttccg gcttcaatgt gattggtgat ctcttcgctg gtcagatctt gtttcttcgc
     1441 gcggatcacg ccgccatctt ccagcaacga tttcagctcg gcttcatcaa gcagctggaa
     1501 gccctgtgcc gcactaccgg agcgaaccca ttcggtcagc gtcagttcaa tcgggttttc
     1561 catgctcaat ggtacaaccg gtaacgaccc caggctttta cgcagtaatg ccagcgtatc
     1621 ttccgctttt ttggcactgg cgcagtccac cataatcaaa ccgttaaccg tgtcgagcca
     1681 catcattgtc tggctaaaac ggctgaaagc acgcggcagc agagagtgca gcacttcgtc
     1741 tttcagcgaa tctttttcgg ttttcttcag cttacgcgcc tgttccgctt ccagtttggc
     1801 gattttcgct tccagcgcct gtttaatcac cggagacggg aggatttttt cttctttgcg
     1861 cgcgcagata acaatttgac cattggcaac gtgcgttaac gcatcgctgt gcgatcccat
     1921 cggaggaacc cagcccatct tcgccatgtc ctggctgccg catggggtaa atgccatcga
     1981 ggctagctgt ttttccatct cttctgcacg cagcgaaatc tcgcggctaa gacggtaaac
     2041 cattaaattt ttgaaccaca gcatgataat ttccacggcc ttgtcgttaa atttagcggg
     2101 catgataacg aattgtcggc ggccttgcat tgccaatccg gttgtccgtc tctacgctat
     2161 tgatattgaa aaaaataagg agagtacagt gcgtataggt atcgatttag gcggcaccaa
     2221 aactgaagtg attgcactgg gcgatgcagg ggagcagttg taccgccatc gtctgcccac
     2281 gccgcgtgat gattaccggc agactattga aacgatcgcc acgttggttg atatggcgga
     2341 gcaggcgaca gggcagcgcg gaacggtagg tatgggcatt ccaggttcga tttcgcccta
     2401 caccggtgtg gtgaagaatg tcaattcaac ctggctcaac ggtcagccat tcgataaaga
     2461 cttaagcgcg aggttgcagc gggaagtgcg gctggcaaat gatgccaact gtctggcggt
     2521 ttcagaagca gtagatggcg cggcagcggg agcgcagacg gtatttgccg tgattatcgg
     2581 cacgggatgc ggcgcgggcg tggcattcaa tgggcggacg catatcggcg gcaatggcac
     2641 ggcaggtgag tggggacaca atccgctacc gtggatggac gaagacgaac tgcgttatcg
     2701 cgaggaagtc ccttgttatt gcggtaaaca aggttgtatt gaaaccttta tttcgggcac
     2761 gggattcgcg acggattatc gtcgtttgag cggacatgcg ctgaaaggca gtgaaattat
     2821 ccgcctggtt gaagaaagcg atccggtagc ggaactggca ttgcgtcgct acgagctgcg
     2881 gctggcaaaa tcgctggcac atgtcgtgaa tattctcgat ccggatgtga ttgtcctggg
     2941 gggcgggatg agcaatgtag accgtttata tcaaacggtt gggcagttga ttaaacaatt
     3001 tgtcttcggc ggcgaatgtg aaacgccggt gagtaaggcg aagcacggtg attccagcgg
     3061 cgtacgcggc gctgcgtggt tatggccaca agagtaaaaa acgtaggcaa ttggcgcatc
     3121 atgcctgatg cgacgcttgc cgcgtcttat caggcctaca aaatgtgcca gaaccgcgta
     3181 gggcggataa ggcgttcacg ccgcatccgg caataagcaa cgcgcaccgt gacagtaatc
     3241 taccccagtg gtttcgccag caccggacta tccgccgctt gctggcgctt atagcgacca
     3301 tacagcagca acgatgacat cgcagcaaac gaaagcaggg cggcaggcag cgccacgtaa
     3361 ttatatgcca gccccagcgt cagcatcata cctccgcaat atgcgccgac ggcgctaccg
     3421 aggttaaacg ctatttgccc acctgcggca cctaataact ctcctccttt ggcattttgc
     3481 agtaacaata tttgcagagg tgctgaaagg gcaaataatc ccgcgcaaca aataaaagca
     3541 aaaataagcg acgttgtttt catgccgccg cagaaaaaga gcatcagcag tgccagtaca
     3601 atgataaagt cagtcactgc tgcaatgcgc agtggtgaat aacgtcctga aatcctgcca
     3661 cttagcatat ttcccagcac catccctagc ccaactaaca tcataataaa ggtcatcgcc
     3721 gtttccgaaa aaccggaaat aaacatcatg tatggcttta cgtagctgaa ccaggcaaac
     3781 acacctgcgt tgccaaacat cgtggcggcg aaaattaacc acggggccgg gctgcgcaaa
     3841 aagtgaaatt gttcgcgcag atttcctttc gcctcgtcgc gaatatctgg cacccaaaaa
     3901 tagaccgatg ccatcaccgc aatattaaaa acagcgat
//
LOCUS       DAZSNM010000273         3929 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZSNM010000273 DAZSNM010000000
VERSION     DAZSNM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.273"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(167..1525)
                     /gene="plaP"
                     /locus_tag="ACHW42_003909"
     CDS             complement(167..1525)
                     /gene="plaP"
                     /locus_tag="ACHW42_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR8945511.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1515..1577)
                     /gene="yoeI"
                     /locus_tag="ACHW42_003910"
     CDS             complement(1515..1577)
                     /gene="yoeI"
                     /locus_tag="ACHW42_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR8945512.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(1738..2721)
                     /locus_tag="ACHW42_003911"
     CDS             complement(1738..2721)
                     /locus_tag="ACHW42_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR8945513.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(2767..3591)
                     /locus_tag="ACHW42_003912"
     CDS             complement(2767..3591)
                     /locus_tag="ACHW42_003912"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8945514.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3674..3928)
                     /gene="yoeB"
                     /locus_tag="ACHW42_003913"
     CDS             complement(3674..3928)
                     /gene="yoeB"
                     /locus_tag="ACHW42_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR8945515.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1060 a         1018 c          923 g          928 t
ORIGIN      
        1 cagatgaatg ttaatgggga aatttttcat gactggtgga atgcagacca ataaccaaat
       61 tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaagaa taaaaaaacc
      121 ggagccaaat gttcattcga ctccggttta ttattagaaa agatgattat gctacgtctt
      181 cgtactgcgg aaccggatta cggaagctct tggtgacgca agccaggtaa atcagaccga
      241 tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc cacagcgcac
      301 caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct ttcagcgtct
      361 tgttacgctt ctcacggatc cagaactgcg agatgaccga caggttaaca aaggtgaacg
      421 ccaccagcgc accaaagtta atcagcgccg ttgccattac caggtcgaag ttgattgcca
      481 gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat ttcgggtgta
      541 cataaccgaa gaagcttttc gggaacacgc cgtcacgacc cattacgtac atcagacgcg
      601 ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag aagatcagcg
      661 cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt gacgcatccg
      721 gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt gcaaagatga
      781 agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg cgctctgcat
      841 ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag aagcacagga
      901 ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac ggacgcgtgc
      961 tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg cccagaatca
     1021 ccgcgatcag cactacctgc aacacgacga ttacagtgtt gacgttcgct acggatttca
     1081 gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac atccacgatg
     1141 gaatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg atcatcggcg
     1201 cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc gggctaatgg
     1261 atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc agcttcccgt
     1321 agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc ggcacatgac
     1381 catccgtgag gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc tgcatatagg
     1441 ctagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc acgcgagagg
     1501 tgtttggagt aacgttatgc gacatggtta ttctccttta cggtgataac cgtcgcgtaa
     1561 gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga tttttagtaa
     1621 atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga agcaaaaaat
     1681 aaccgacgct aattaaacgt cggtttttaa tcattttttg acagcggcgc attgtgccta
     1741 aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc tttgaatggc
     1801 tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg ctaaaatttc
     1861 atctcgccac cagccgctgg caagccctgc ggtctgttca ttccagccaa tccagacagg
     1921 ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat cgagaaaacg
     1981 ttgcgccaga taccgtggta aatagccgca gccaaggccg ctaatttgca gctccagttt
     2041 ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg aggccgtaga
     2101 agcgcctgcc tgcgcagtat cccccaccac aatcgcccga tatcgtttaa tgatacgacg
     2161 gtttaacggt tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa agacttgttc
     2221 aagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat gcatcgcgcc
     2281 aacgataata tccgctcgcc cctgagtcag ggcatcccag gaaccaccga gtacgccatt
     2341 gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatga gaggcgcaag
     2401 cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc agccttcatg
     2461 cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc gccctttctc
     2521 taataacatt tttccggtgc gggtgaattt agcgcggtgt ccgctacgat caagcaattg
     2581 aatatttagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg gcgtcttgta
     2641 gagtttggct gacgccgccg caaaactgcc ctctttttct atggcatcga ggatcatcaa
     2701 cacatccagc agtggtttca tacacgcccc cttgcagtgc atgcggcgtc ctgctggcgt
     2761 gatcgtttac tccaacggca ttaccagcgg atcgggatac tggtattcaa atcccagttc
     2821 attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac tatttctgaa
     2881 ctgcggaggt tgcagcccca gtaaacgggc catctgcgga tagaaaacat tacgcgcagg
     2941 gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta acaacagagt
     3001 gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac catcaggggc
     3061 ggtttttccg gcaaagaagc gtcccggatg acgtcccggt ccgaccaggc ccgcaagacg
     3121 cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt cttctaatac
     3181 tcgtccactg ttggttaccg gattacgcgg ggtagtttct ttaaccgtgc cttgcgcgtc
     3241 gccatagaca gatgttgagc tggtaaaaat aattcgggga atacgatggg ccagcgcgct
     3301 atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc tacgacgtgc
     3361 cggaagcgta atgaccagcg catcggcatc cataagggca tccagatcgt cagaatcgca
     3421 aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc gggccgcttc
     3481 gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg aaagtgacat
     3541 cgccagcggc atacctaacc accctaaccc gacaattgcg acctttttca tccgttatct
     3601 cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca attcattcta
     3661 tgaatgaatc tgttcaataa tgataacgac atgctgcaat gagcagtgaa tcatcagtaa
     3721 ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga ccagaaacct gacaaattat
     3781 gtttcagggg ttctggtttc cccttacctt caaatggtgt tctgcgggta tctttgataa
     3841 gttcattgat ctttttaaca attcgcttat ctgtttcctg ccagtacaga taatcatccc
     3901 atgattcctc agaccagatt agtttcact
//
LOCUS       DAZSNM010000274         3879 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZSNM010000274 DAZSNM010000000
VERSION     DAZSNM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.274"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1967)
                     /gene="speA"
                     /locus_tag="ACHW42_003914"
     CDS             complement(<1..1967)
                     /gene="speA"
                     /locus_tag="ACHW42_003914"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR8945516.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYL"
     gene            complement(1976..2107)
                     /gene="yqgB"
                     /locus_tag="ACHW42_003915"
     CDS             complement(1976..2107)
                     /gene="yqgB"
                     /locus_tag="ACHW42_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR8945517.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            complement(2201..2344)
                     /gene="yqgG"
                     /locus_tag="ACHW42_003916"
     CDS             complement(2201..2344)
                     /gene="yqgG"
                     /locus_tag="ACHW42_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR8945518.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2455..2706)
                     /gene="yqgD"
                     /locus_tag="ACHW42_003917"
     CDS             complement(2455..2706)
                     /gene="yqgD"
                     /locus_tag="ACHW42_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR8945519.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2762..>3879
                     /gene="metK"
                     /locus_tag="ACHW42_003918"
     CDS             2762..>3879
                     /gene="metK"
                     /locus_tag="ACHW42_003918"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR8945520.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTD"
BASE COUNT          977 a         1047 c          961 g          894 t
ORIGIN      
        1 tcaagataag tataaccgta caaacctgcc tcgaactctt caaggaactg ttgttgcagt
       61 tcagcatcaa gatcggtttt cttcacttga tcgcggaact gggttaacag cgttttcgga
      121 tcgagctgta catattgcag catgtccgcc acggtatcgc cttcgtcaga cagttctact
      181 tctacgctac cgtcagggaa cacgaacacg tcaactgctt cggtatcacc gaacaggttg
      241 tgcatattgc caaggatctc ctgatatgca ccgaccataa agaaaccgag catcggcgga
      301 ttctctggat cgtactccgg cattggcatt gtcgtggcaa taccatcacc atcaatatag
      361 tggtcgatag caccgtcaga gtcacaggta atatccagca gcacagcgcg acgttccggc
      421 acttgatcca gcccttccag cggtagaacc gggaacaact ggtcgatccc ccatgcgtcc
      481 ggcattgact ggaacagcga gaaattgacg tacattttgt ccgccatacg ttcctgcagt
      541 tcgtcgatta tcggacgatg cgcacggttt tgcggatcca gttgcttctg cacttcatgg
      601 cacatgctca gatagagttg ttctgcccat gcacgttctt gcaggctaaa ggtgccggaa
      661 gagtagccga tatgaatgtc gtgcagatcc atctggctgt cgtgtaacca ttcacgcaaa
      721 gaacggcgag ttcccggttc gtgcatttcc tgccaggttt cccacatgct ttgcagcgca
      781 cgcggcgcat cttctacggg cgcggtcggc accgtatatt cgttacgttc cacgccgatg
      841 atattcgaca ccagcacggt atggtgcgca gtcaccgcac ggcccgattc ggtgattacc
      901 gtcggatgcg gcagaccatt ttcttcacac gcatcgccaa tcgcccagat gatgttgttg
      961 gcgtattcat tgaggccata gtttacggag cagtcagact gcgaacgggt gccttcataa
     1021 tccacgccca gaccgccgcc gacgtcgaag cactgaatat tgacgcccag cttgtgcagt
     1081 tccacataga aacgcgcgga ttcacgaacg cctgtcgcga tatcgcgaat attcgccatc
     1141 tgcgaaccga ggtggaagtg cagtagttgc aggctgtcga gacgcccggc ttcacgcagg
     1201 gtttcaacca gttgcagtac ctgagtcgca gccaggccga acttcgattt ttccccgccg
     1261 gaggactgcc atttacccga gccctgcgaa gccagacgtg cacgcacgcc cagacgagga
     1321 acgacattca gacgttctgc ttcatccagc acaatggcga tttctgacat cttctcaatg
     1381 accagataga ccttgtgccc catcttctcg ccaattaatg ccaggcggat atattcgcgg
     1441 tctttataac cgttgcagac gatgacgcta cgagtcatgc cagcatgtgc cagcactgcc
     1501 atcaactcgg ctttggaacc ggcttccaga cccagcggtt cacccgaatg aatcagggac
     1561 tcgatcacgc ggcggtgctg gttaactttg atcggataaa caaggaagta atcgccgtta
     1621 tagccgtagg attccctcgc acgtttgaac gcggcgttaa tggaacgcaa acggtgctgc
     1681 aggatctgtg ggaaacagaa cagtgcaggc agacgctgac cctgtgcttc acgagttttc
     1741 actaactgcg cgagatcgac gcgagcttcc gggacgtccg ggtccgggca cacgctgatg
     1801 tggcccagct cgttaacgtc atagtagtta ttgccccacc aggcaatatt gtaagtacgc
     1861 agcatcttgc tggcttcctg ggagctcatt gcaacctcct gcatggagcg tagtacaccg
     1921 tgttcgcccg ctgacgaagg caaacccata gacatgtcgt cagacatagc gaacctcaaa
     1981 ttattttatt aagtgtaaaa cagttaacga ctgtcgcagc ctggaactgc gataacaacc
     2041 cataagcaca tggattttcc agcagtgtat gctgacgctc cgactgcgca accggtttct
     2101 ttttcataac attattaagc acataaccga acgtaagtgt gacagttcgg cgaaaccacg
     2161 agaaaactct tgtttttaca agagcgccct tgttcagtcc tcagtaactg taaccagctc
     2221 ttgaatcctg agaagcgccg agatgggtat aacatcggca ggtatgcaaa gcagagatgc
     2281 agagtgcggg gaacgagtct tcaccagaac ggtgagacag gttaagcagc agacaacggt
     2341 tcattatttc gtatcacctc cacggccgcc tgttaagacg aacccacaag ccaaaactct
     2401 gatttcaacc cggctggaag tggcaacacg aaagaaacgt cgtgtgcttt ttatttaagc
     2461 cgcgcgccgc gttttatacc ccacaatggc agaaaattgc aaaagataaa tacgcagaat
     2521 gccggcattg tcaggaaaaa tttccagcca cgtttttaac agaatgagac acgattcaaa
     2581 aaaaagtgga aatcgggtga agaattgacc taaaatagcc atccagatgt taatccatcc
     2641 ataccgatta acactcagac tgccagtgtt tttaacctgc agagtcgtgg taggatccgc
     2701 taccacagaa aatccacaca acagtttgag ctaaccaaat tctctttagg tgatattaaa
     2761 tatggcaaaa caccttttta cgtccgagtc cgtctctgaa gggcatcctg acaaaattgc
     2821 tgaccaaatc tctgatgccg ttttagacgc gatcctcgaa caggatccga aagcacgcgt
     2881 tgcttgcgaa acctacgtaa aaaccggcat ggttttagtt ggcggcgaaa tcaccaccag
     2941 cgcctgggta gacatcgaag agatcacccg taacaccgtt cgcgaaattg gctatgtgca
     3001 ttccgacatg ggctttgacg ctaactcctg tgcggttctg agcgctatcg gcaaacagtc
     3061 tcctgacatc aaccaaggcg ttgaccgtgc cgatccgctg gaacagggcg cgggtgacca
     3121 gggtctgatg tttggctacg caactaatga aaccgacgtg ctgatgccag cacctatcac
     3181 ctatgcacac cgtctggtac agcgtcaggc tgaagtgcgt aaaaacggca ctctgccgtg
     3241 gctgcgcccg gacgcgaaaa gccaggtgac tttccagtat gacgacggca aaatcgttgg
     3301 tattgatgct gtcgtgcttt ccactcagca ctctgaagag atcgaccaga aatcgctgca
     3361 agaagcggta atggaagaga tcatcaagcc aattctgccc gctgaatggc tgacttctgc
     3421 caccaaattc ttcatcaacc cgaccggtcg tttcgttatc ggtggcccaa tgggtgactg
     3481 cggtctgact ggccgtaaaa ttatcgttga tacctacggc ggcatggcgc gtcacggtgg
     3541 cggtgcattc tctggtaaag atccatcaaa agtggaccgt tccgcagcct acgcagcacg
     3601 ttatgtcgcg aaaaacatcg ttgctgctgg cctggccgat cgttgtgaaa ttcaggtttc
     3661 ctacgcaatc ggcgtggctg aaccgacctc catcatggta gaaactttcg gtactgagaa
     3721 agtgccttct gaacaactga ctctgctggt acgtgagttc ttcgacctgc gcccatacgg
     3781 actgattcag atgctggatc tgctgcaccc gatctacaaa gaaaccgcag catacggtca
     3841 ctttggtcgt gaacatttcc cgtgggaaaa aaccgacaa
//
LOCUS       DAZSNM010000275         3871 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZSNM010000275 DAZSNM010000000
VERSION     DAZSNM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.275"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..442)
                     /locus_tag="ACHW42_003919"
     CDS             complement(<1..442)
                     /locus_tag="ACHW42_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR8945521.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLM"
     gene            complement(455..2534)
                     /locus_tag="ACHW42_003920"
                     /pseudo
     CDS             complement(455..2534)
                     /locus_tag="ACHW42_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(2555..3181)
                     /locus_tag="ACHW42_003921"
     CDS             complement(2555..3181)
                     /locus_tag="ACHW42_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR8945522.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(3637..3846)
                     /gene="fumD"
                     /locus_tag="ACHW42_003922"
     CDS             complement(3637..3846)
                     /gene="fumD"
                     /locus_tag="ACHW42_003922"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR8945523.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         1000 a          993 c          816 g         1062 t
ORIGIN      
        1 gcatcagcat ggcgactttg taaggacgag gatacgtctg gcactcaaaa cgaacggcgt
       61 gtgcaatggc gcgacaaatg tcctgctcca caacttgcaa agacatcgcc gcgtagtttt
      121 cactcatggc ctgcgttgag taatagtaat catcctgact tcccgtcata caagcgatat
      181 ccgcgtagtc tccgttctgc ttaatgcttt ccagcaaaac tgacagttcc tcttctgcaa
      241 ccgaataggg tggcgagaga aacacggtgc gcgccaccag ctgcccagcc gcagaatgct
      301 ggcgaatgaa ctcagccagg atttcagcag gttctggttc ggttaccgta tcaacggggt
      361 gacgcatccc ctgcaaccat tgccgcttcg cctcgtcaac ttcgctgact ttcgctaacg
      421 gcaattcatc ctgatggttc atcttaccca ttccttacgc aggcagtaga ttgtgtgctg
      481 ccagatcggc ggcaatatct tccagcccca gacgctgcaa tgtttcgcgg gttggacaac
      541 caagctgtgg gtcccagccc atttctttgt agaacatcgt gagcgaagca tgcatatcgt
      601 cacgatccat tttgtcagta ccttcagtaa agaccgggat ctgcggatcc ttgtcgaaaa
      661 cccaggaaca gataagatcg tgttcattac gcatatcttt ggtttgcatc ggttttaccg
      721 tgtaggcacg atgcaacgta aagatacgct ctgcagctaa atctaatttt tcctgagtca
      781 tctcttcgcc ggtgatcgct ttgaagaatt tggcttcaag cgccagatcg ccccggtaat
      841 tacggctttt cagcggcgaa acggtcattg gccagaccca gttgcacaga gtgacggcgt
      901 tatgcaaaca gacccgcaac agcgaccact ttgcatattt aatttttgcg tcattgattg
      961 gcgtgtagtt tttggtttca tcgtaagcat cttcagaacc aaaaagttct ttcgccactt
     1021 cacgttgcag tttcaatggc aagccggaac caataaagtt gatatgggta tgcgtcatgc
     1081 agtcacggtt gaacatacac ttaacaatgg aaccgacctg cgctgacgct tcattggcat
     1141 ggtgaaccgg atagccaaac ggcgaccaga gtttattttt cgcgtagccc cagtactctt
     1201 tacccaaatt ccagcgttct gcgatggcat atgaaccatc agccaggtga ctcagctcac
     1261 ccacacgatg cgccagacgg tagtaaaaat ctttaatgaa gttaacgtca cccgcttcca
     1321 gttgatccca gcgaatttct gcatactctt cagctggcag aacacgcttg aacacacctt
     1381 tgctgtaaca ataagtaaag tcgcgatgca actgcccgta gttacaccat aggccgtagt
     1441 catcgaacaa attcagacca accaggttac caatcacacg accatcgtct ttatcttcaa
     1501 aatctttcgg cccgttcggg aagatggtgg tatggacaaa gtttgctaca caagtgttac
     1561 cacctgtgct gggcacgcca aactctttca cccgaggaat attcatttgg gtcatacaac
     1621 ggatcgggca agagtggcag ccgctcattt ttactgtgta tttctctgcc gccggtccta
     1681 agtcaaaaac ggatttatag gtacgaaagc cgaccgtatt ctgattgcct ggcggaattt
     1741 cacccgtttc aatcggacca ccttcagccg cgccccaaaa cagcccttta cgtgctgtcc
     1801 agcgtgactt gggatctgcg tacttggcac gacatggttg ttattcgcac caataagttc
     1861 agtcatcatg taatcattga gacgcttcat ctcctgacga tcggcaatgt tgaccccttt
     1921 cgtcccttca accgcaatcg ctttcaggtt tttcgaaccc attattgcgc cagttcccgc
     1981 accgccgctg tggttacggc tattcaacat gccagagaga ggaataaggt tttccccagc
     2041 ctgaccaata gccgccacac aggtttccgg gctggtcaat cgacaaattt cttccgtcgt
     2101 tgcgcgcgtc ccttttcccc ataagaaatc ggctttttcc aggctaactt tgtcatcttt
     2161 aatcttcagc cataccggtg atttcgcctt cccttcgata ataatgacgt cgtatccagc
     2221 gaatttcatt tgcgctgcaa aaaagccacc catatgggca tcgcgactaa atttcctttg
     2281 gtaaaagtag aaagtgaggt gatatttacg cgagaactac agggggcacc agatccaggt
     2341 aatgggccgg tagcaaagac taatttattc gcttcatcga aaggtttcgt gcctagcggt
     2401 acttcgtcat acataatttt gtagccgaag cccatgccac cgacaaaact tttaaactta
     2461 ctggaatctt cgagggtaat atttcctgtc gtgagattga ctcttaatat attacctgtc
     2521 caaccgttag ccatgatttt ttcctttgca agattcacac agtaatatct ttccactcga
     2581 taatttttaa cgcccctgtt gggcaggcgt ttgcgcattc accgcataac acacatttcg
     2641 aggatttttt actttcggta tttacggtgg ccatcatcca cgggcatgcc gtagtacagg
     2701 cgctacagcc aatacaacgt ttatgatcga cggtaataca gccttctttc tgctgccagg
     2761 taatcgcacc aatcgggcag acgttcatgc attgcggctc tttgcattga cggcaggtgt
     2821 ccgcggtata gttgagatcg ccatacaggc cgccgccaga gccaaccccg ttgtcgccaa
     2881 agaaataatt gcgatggatt ttgatacggg agaagaatgt ccctactgag ccatcgttga
     2941 agttggtaca agagatttca cagcggtggc aacccgtgca tcgcgctcgc tgggtcacca
     3001 gcaccccttt gggtgtattt atcaacccta ctgtgccgct atcaatatct tcctgtttgc
     3061 aacccaaaag tgacagtaac gcaggggcaa ttgttagacc agcaaggcct tttcctgata
     3121 tacgcaagaa ttctaatcgc gttaagccaa tatctaatag tggacgatca accgggttca
     3181 tttatttcgc atcctcttcg caaacacagt gctgacaatt cagcagcctg atattaagca
     3241 ttcaactaat acgctgctga ctctgtcccc aacaccagat attttcccta acccgatagt
     3301 gtatgagaaa agatttaacg ccgataaact aatcctttat ggttaggaga aaaaataatg
     3361 tgattattct gagcccttaa cattgatcgt tatcaattaa aattacaaag caagaaatta
     3421 taagcgaact gatatttatt atcatttgaa ataaatttaa cttaagcgac aatcatttgt
     3481 gacgttcatt gcagaaaata aaaataacat tatcgctata aattaaaata cataccgaaa
     3541 ttcgtataat ttcagcagtt gttatttaga atgattaact ggaccgcaac tgaagcaaga
     3601 atatcaggat gaagcttacc gcctcatcct gaatggtcat tttaccagtg cgttaatgac
     3661 ggtttccatc gcctgctttt ccagctcact gcgctgaatg cgtttattgg ctaatgctgt
     3721 acgtaatacg cctgcgataa caatatgctt tggttcctgc cccagatcgc gcatttccag
     3781 cacaacttta ccgacaactc tgcacatttc tcggtacagt tcatcctctt ttgtccgatt
     3841 tcccattctt tatacctatt tatcatgcca a
//
LOCUS       DAZSNM010000276         3801 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZSNM010000276 DAZSNM010000000
VERSION     DAZSNM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.276"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1852)
                     /gene="rsxC"
                     /locus_tag="ACHW42_003923"
     CDS             complement(<1..1852)
                     /gene="rsxC"
                     /locus_tag="ACHW42_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR8945524.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVD"
     gene            complement(1845..2423)
                     /gene="rsxB"
                     /locus_tag="ACHW42_003924"
     CDS             complement(1845..2423)
                     /gene="rsxB"
                     /locus_tag="ACHW42_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR8945525.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(2423..3004)
                     /gene="rsxA"
                     /locus_tag="ACHW42_003925"
     CDS             complement(2423..3004)
                     /gene="rsxA"
                     /locus_tag="ACHW42_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR8945526.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(3081..3521)
                     /locus_tag="ACHW42_003926"
     CDS             complement(3081..3521)
                     /locus_tag="ACHW42_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR8945527.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(3607..>3801)
                     /gene="ydgT"
                     /locus_tag="ACHW42_003927"
     CDS             complement(3607..>3801)
                     /gene="ydgT"
                     /locus_tag="ACHW42_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR8945528.1"
                     /translation="LKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELV
                     SGGRLFDLGQVPKSVWHYVQ"
BASE COUNT          956 a         1018 c          971 g          856 t
ORIGIN      
        1 gatcgacctg ttcttctggt tccgcattag cctgttgctg ttccagcttg cgcgctttgg
       61 cacgggcaat agcggcttcg acggcggctt tgcgcggatc gacctgttct tctggttccg
      121 cattagcctg ttgctgttcc agcttgcgcg ctttggagcg ggcgatagct gcttcaacgg
      181 cagttttacg tggttcagca acggttgctg cgtcgttagt ttgctccagt tctgcctgtt
      241 tcgctctggc ttgcgcttta cgggcttccc gttctgcaat aattgcactg ttatccgggc
      301 gttcgcccgc tttaatcaca ataggctgtg tagcctgggc ctgtttctct ttcacccgcg
      361 ccagagcggc agcaatcgca tctttatctt tggctgcagg ttgaacggct gcgctcttat
      421 gtcgttcaag gcgagtcgct ttttcgcgct ccagacgagc ctggcgcgct tcgaaacgcg
      481 ctttggcttc tgcggcgcgc ttttcttcct gacgaatagc cgcaatttca gctttttcct
      541 gacggaaata ttgcaccaag ggaatattgc tcgggcaaac ccacgcgcaa gccccacatt
      601 caatgcagtc ggcaatgtta tgcgtggtag ctttatcgtg ttgctgacct ttgctgaacc
      661 agtacaactg ttgcggcaaa agatccacag ggcaggcgtc agcacaggcg ctacaccgga
      721 tgcagctttg ttcttcctgt ggttcgccaa gttcattggc agagggagcc aacagacagt
      781 tggtaatctt tacgaccggg acatccagcc atggcaaggt aaagcccatt agcgggccac
      841 ccataatcac catttgatcg gcagaggggc agaatccggc atcattcaat aaatgacgca
      901 ctggcgtccc cagccgtgcc cagacgttgc ccgggcgagc gattgcttcg ccagtcaggg
      961 ttacaacacg ctcggtaatc ggctcgccat caataacggc acgtttcact gcataagcag
     1021 tgccgacgtt ttgcattaat acgccgatat cggatgaacg cccgccatgt ggaacctgct
     1081 tcccggtcag aatgtaggtt aattgtttag caccgccaga aggatatttg gttggaatca
     1141 cccgcagaga aatatcgtta gagtccgcca gcaccgcgcg cagcatggaa atcgcctgcg
     1201 gtttgttatc ttcaatgccg ataagaattt cgcgtggctg cagaatatgc gcaagaatgc
     1261 gaataccctc tacgacctga gccgcgcaat cctgcatcaa acggtcatcg gcggtaatgt
     1321 acggctcgca ctcagcagcg ttgataatca acgtttcaat cttatctcca ccaccctgca
     1381 atttaacgcc tgtcgggaat ccggcaccgc ccagcccggc aacaccaaac tgatgtatgc
     1441 gctcgattaa ctcttcgcga ctgcgagagc gataatcgac ccagccgtcg cgcgggatcc
     1501 agcagtcttc accatcggca tcaataatca cgcttaattc agctaaagct gaaggatgag
     1561 ccgtagagtg gggcgcaata gccgtaacgg tacccgaggt gggcgcgtga acaggcagca
     1621 ttttgccgcg accacgggta agcggctggc cgcgcaatac tttatcgccg acgctaacgc
     1681 acaactcacc ttcagcgcca atatgctgtt tcagtggaat aacaaaatgc tgcgccagta
     1741 gtacctggcg caggggggta ccgttggtct gggttttcat ctccggtgga tggatgccgc
     1801 cgttgaaatc ccagatttta ttttttctga atgcagagaa taacttaagc atggtgttcc
     1861 acgggaatga tacgcacggg aatggtgttc agatcccatt tccaggagtc aggtgtttct
     1921 gcgaccggtt gcaacgagat gcagtgcgtc gggcacggat caacacataa attgcagccc
     1981 gtacagagat cactcattac cgtatgcatg acacgggtag cgccaacgat ggcgtctacc
     2041 ggacaagcct gaatacattt agtgcagcca atacagttat tttcatcaat aaccgccacc
     2101 atccgcgcag gcgttagctc ttgcgcttcg ccatccagcg gctgcggctc gacattaagc
     2161 aactcggcaa tttttagcat cacagcttcg ccacctgggg cgcaacggtt gattttttca
     2221 ccgttacagc tgatggcttc cgcgtagggg cgacagccgg gataaccgca ctgaccacac
     2281 tggctctgcg gtaagatttc gtcaattttc tcaacgaccg gatcgtcttc caccgcaaaa
     2341 cggcgggagg cataacccag aatggcgcca aacgccaggc ccagcaggct cacggcggca
     2401 acggcaatcc agatagcatt cattacaact tcaccaaacc actaaagccc ataaaggcca
     2461 gagacataag acctgcggta attaacgcaa tggcattacc gcgaaaaggt gccgggacat
     2521 cagccacagc aaggcgttcg cggatggcgg cgaagagcac catcaccagc gagaaaccga
     2581 cagcggcgga aaaaccgtac agcgccgact gcaagaaatt gtgcccgaga ttgatattca
     2641 gcaacgccac gccgagcact gcacagttgg tggtgataag cggcaaaaaa atccccagca
     2701 gacggtaaag caccgggctg gttttgcgca ccaccatctc ggtgaactgc acgaccacag
     2761 caatcaccag aataaatgcc agggtgcgca ggtaaatcag attaagtggg atcaaaatcc
     2821 acgtatcgat aagccaggcg caaatagacg tcagcgtcat cacaaacgtt gttgccagcc
     2881 ccatgcccat cgcggtttcc agttttttgg aaacccccat aaacggacag agaccgagaa
     2941 acttgaccag tacaaagtta ttgaccagta cagttccgac aaacagtaac aggtagtcag
     3001 tcatttttcg gcctgaaata aaaaagccgc ctattatccg ttaatccaga gcaggcgaca
     3061 acaggttaac tgtaaggtta ttacggattc acaaacgtcg cttttacccg cgacgagcgt
     3121 ttgaaatagg ggacgatcaa tgcggttgcc aacaaagtaa acagcaactg gcgaacggct
     3181 atgccaactt ccacgggtga aaaagcgaat gcttttactg ccagcaatac ggaaataagc
     3241 agccagataa tatagtgttt aggaacacaa cgccgacgtt taaagaaagc gatggtcaac
     3301 cacagtgtgt aataccacat ggcgatagcg gtaataaaag aaacgcccca taagatttgt
     3361 gtcgtcagtg cttgtccgcc gagcgtttga aatgtttgcg gagaagataa cgcagcggta
     3421 tacagcaaca acgccagcgt cgtactcaac agagcgacta aaagccaggc caacgggcca
     3481 agcaaccagc ctccaatacg ttgtggcgtt gtggtggtca taggttctcc agaatagcga
     3541 cgcaaacagg caaatctaat tttaggggcg ttattataag gcttttataa ccgatcgcta
     3601 ctttctttat tggacatagt gccagacgga cttcggtacc tggccgaggt caaacaaacg
     3661 cccgccagaa accagctctg cgcgacggtg atcggcagca cgatacatat tgatcagttc
     3721 ctgatcgtcc gtcagggtgt aattaagatg gtcgtagagt ttttccagac tttcgagtga
     3781 actgattttg cgaaatttta a
//
LOCUS       DAZSNM010000277         3776 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZSNM010000277 DAZSNM010000000
VERSION     DAZSNM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.277"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..441)
                     /locus_tag="ACHW42_003928"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="ACHW42_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="ACHW42_003929"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="ACHW42_003929"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR8945529.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2369)
                     /gene="araH"
                     /locus_tag="ACHW42_003930"
     CDS             complement(1383..2369)
                     /gene="araH"
                     /locus_tag="ACHW42_003930"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR8945530.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..>3776)
                     /gene="araG"
                     /locus_tag="ACHW42_003931"
     CDS             complement(2384..>3776)
                     /gene="araG"
                     /locus_tag="ACHW42_003931"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR8945531.1"
                     /translation="NGAGKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVA
                     IIYQELHLVPEMTVAENIYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKY
                     LAIGQWQMVEIAKALARNAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSH
                     RMEEIFALSDAITVFKDGRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEE
                     RLRLDAVKALGVRTPISLAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQ
                     QPIDIRKPSHAIAAGMMLCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWE
                     ENNADQHIRSLNIKTPGAEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVG
                     AKHEIYNVIYALAAQGVAVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQA
                     LSLAMPKVSQAVA"
BASE COUNT          950 a         1047 c          902 g          877 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattt
//
LOCUS       DAZSNM010000278         3761 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZSNM010000278 DAZSNM010000000
VERSION     DAZSNM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.278"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1040
                     /gene="tsuA"
                     /locus_tag="ACHW42_003932"
     CDS             <1..1040
                     /gene="tsuA"
                     /locus_tag="ACHW42_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR8945532.1"
                     /translation="GLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLIAISA
                     QSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGS
                     WIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVLLVITLWV
                     VMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMF
                     GLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRAADAQTTL
                     RSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQ
                     RKARLATAAAN"
     gene            1054..1281
                     /gene="tsuB"
                     /locus_tag="ACHW42_003933"
     CDS             1054..1281
                     /gene="tsuB"
                     /locus_tag="ACHW42_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR8945533.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1324..2748)
                     /gene="sbcB"
                     /locus_tag="ACHW42_003934"
     CDS             complement(1324..2748)
                     /gene="sbcB"
                     /locus_tag="ACHW42_003934"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR8945534.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            2930..>3761
                     /locus_tag="ACHW42_003935"
     CDS             2930..>3761
                     /locus_tag="ACHW42_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8945535.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     E"
BASE COUNT          891 a          888 c          991 g          991 t
ORIGIN      
        1 gcgggctaat ttgtggtgct ctgctgggat ttgtcatgca gcgtgggcgt ttttgcctga
       61 ctggtggttt tcgcgatatg tatatcgcga aaaataatcg catgttttac gccttgctga
      121 ttgcaatttc ggcacaaagc gtgggggttt ttgcgttaat tcaggcgggc ctactgactt
      181 acgaagccgg ggcgttcccg tggcttggta ctgttatagg tgggtatctt ttcgggctgg
      241 gaattgttct ggcgggtgga tgtgccaccg ggacctggta tcgcgcgggt gagggattga
      301 tcggcagttg gatcgcgctt ttcacttata tggtgatgag tgcggtgatg cgttctccac
      361 acgccagtgg tttaaatcaa accttgcagc actacactac tgaacataac tctattgctg
      421 agacttttaa tttgtctgtg tggccgttgg ttaccgtttt gctggtgata acgctctggg
      481 tggtgatgaa agagttgaag aagccaaaac tcaaagtcgc gaccttaccg ccgcgccgaa
      541 ccgggatcgc tcatattctg tttgaaaaac gctggcatcc ctttgtcact gctgtactca
      601 tcggtttgat tgcgctttta gcctggcccc tgagtgaagc aaccggacgc atgtttgggt
      661 taggagtcac ttccccaacg gccaatattc ttcaatttct ggtcgcgggt gacgtgaaat
      721 acattaactg gggcgttttc ctggtgttag ggatcttcgt ggaggcattt attgcagcca
      781 aagcgagccg tgagttccgc gttcgcgcag ctgatgcaca aacaacatta cgtagcgggt
      841 taggtggtgt actgatgggc ttcggtgcca gtattgcagg tggttgctct atcggtaatg
      901 gactggttat gactgcaatg atgacctggc agggctggat tggccttgta tttatgattc
      961 tcggagtctg gactgcgtcc tggcttgtgt atgttcgacc gcagcgtaag gcgcgactgg
     1021 caactgctgc ggcaaattaa gtaaggtgta aagatggcga ttaaaaagct ggatgtggtg
     1081 acgcaggtct gtccgtttcc actcatagaa gcaaaagcag cactggcaga aatggccagc
     1141 ggcgatgagt tggtaattga gtttgattgc acccaggcaa ctgaagcaat cccacagtgg
     1201 gcggcagaag aaggacatgc catcaccgat tatcagcaga ttggcgatgc agcgtggagc
     1261 atcaccgttc aaaaagcctg actcaacatt gtcctccgcc gtaccagcgg cggcggcttc
     1321 aaattagacg atctcttccg cgtactgcca aagtgctttt aacagcgcca ctttctcttt
     1381 gtcatcggca tattgttgta ccagcatctg caattcatca gcataaccct gcaaaaactc
     1441 tggcgtgaag acctggtggc ggtgctccag ccagcgttgc tgctcggcat aatccagcgt
     1501 ccccgggaag ttgcgtgccc gataattgaa caacaacttt tcaattcgtt tatcaacaaa
     1561 agtgatatcc agtgccggta aattacgcgg ctcggtttcc agcacaattt tcattgctgc
     1621 acgatcggcg tcactgaaaa agccattata aagctgtgca tccacattat ctgaaggtgt
     1681 aaacggttcg gcttccgcga atatcgctac cactttttca cgcacttgcg gattttcacg
     1741 cagaattttt aggttatcga ggcaatgctg acgattaatt cccagtcggt cggcatcttc
     1801 cgggcgtagc gtattcgcct gggccagtac cggacattta ttgatatgca ccagcttaac
     1861 cggaacggcg gcgttatcgc caagatcggc ttttgcggta tataaacgct cgcgcaatgt
     1921 gtcgatatcc agctccagta atggcgaaat gtctcctgcc aaatccgcca taattacggc
     1981 attgcgattt tcaggatgcc acgccagcgg tgccacccag ctggtattgc cacgccatgc
     2041 cccaaacatt ccggaaacgt gagccagggg tttcatctgc ggaacatcaa tcaacgccat
     2101 cagtttgtgt ttattacgat gggtaaagag ataatcaaac aggcgtggct gacgcgtttt
     2161 taccagtttc gccatcgcaa tagtggcgta cacatcagcc atcgcatcgt gggcgttgct
     2221 atgttcaata ccattcgctt tggttaaatg ctcaaggcga aagctcggta gaccgtcatc
     2281 attttcaggc cagtttattc cttccgggcg cagggcataa caggcacgca taacatccag
     2341 taaatcccag cgcgagttat catgctgcca gctccaggcg taaggatcgt agaaattacg
     2401 ataaaaaacg ttgcgtgtga cttcgtcgtc gaaacgcaca ttgttgtagc ccagaataca
     2461 ggtcttcggt acggtaaaaa gcgagtgaat acgggcggca aacgcggctt cgttttctcc
     2521 tttcgcccgt gcctcctgcg gggtaatacc ggtaattaat actgctccag gccggggtaa
     2581 atagtcatcc gcgggcttgc agtaaaagac ttcgggttcg ccgatgacat tgaattcgtc
     2641 atcggtgcga atggctgcga actgtgcagg gcgatctaac gcggggtgcg tgccaaaggt
     2701 ttcgtaatcg tgaaagagga aggtaggttg tttatctgtg tctgtcattg tctagcattg
     2761 tccactgtag ttaattaact gtctgttata actcgaaaag caaccctttt ttcacctgag
     2821 tatgtctacg aacgtcaaat atagttgttt actgtgcgct gttgtccatg tgatattgtg
     2881 taccagatgt gtgccaaact attatttctg agcgtaccaa atattattta tggctataag
     2941 cgacacaaaa ctgcgtacta tttatggtaa accatattcg ggcccacaag aagtggctga
     3001 tgccgatggc ctcagcgtac gaatttcacc gaagggggtc atccagttcc agtaccgcta
     3061 tcgctggcat ggcaagccta atcgacttgg gcttggtcga tacccatccc tgtctttgaa
     3121 ggatgccaga cagatcactg ctgacttgcg aaagctctat ttctcaggaa cggatccacg
     3181 cacctatttt gaagagaagg tggagaactc catgacggtc gcccagtgtc tcgactactg
     3241 gttcgacaac tacgtctcta caactctcag agaaaagacc caggcacttt atcgatcaac
     3301 ggttatgaag cgcatgcatg acgccttccc taatcgtccg gcatcttcta tcacggttaa
     3361 gcaatgggtt gacctgctta ccgaagaaga aaaagataat ccacgccgag caaggcaggt
     3421 gctaagtcaa ctaagatcag caattagttg gtgcatgcgg cgtcagttga tagatagttg
     3481 cgcaattatg agcatccagc caagggactt cggctcccgc gctgaggtag gggatcgggt
     3541 actgtcgtat cacgaactgg ctaagatttg gcttgctatt gaaagaagcc gtgcgtctac
     3601 gtcaaataag ctccttcatc agatgcttat gctgtggggg gcgaggctct cagagcttag
     3661 gctggcaaca aagacagaat ttgacctgct ggacaacgta tggaccgtac cgaaagagca
     3721 tagcaagatg ggtaatgtta tccgccgtcc aatcttcgaa c
//
LOCUS       DAZSNM010000279         3757 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZSNM010000279 DAZSNM010000000
VERSION     DAZSNM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.279"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..757)
                     /gene="repA"
                     /locus_tag="ACHW42_003936"
     CDS             complement(<1..757)
                     /gene="repA"
                     /locus_tag="ACHW42_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR8945536.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAAAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTA"
     gene            complement(750..824)
                     /gene="tap"
                     /locus_tag="ACHW42_003937"
     CDS             complement(750..824)
                     /gene="tap"
                     /locus_tag="ACHW42_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR8945537.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(1069..1317)
                     /locus_tag="ACHW42_003938"
     CDS             complement(1069..1317)
                     /locus_tag="ACHW42_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR8945538.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            complement(1602..1751)
                     /locus_tag="ACHW42_003939"
     CDS             complement(1602..1751)
                     /locus_tag="ACHW42_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR8945539.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            complement(1998..2471)
                     /locus_tag="ACHW42_003940"
     CDS             complement(1998..2471)
                     /locus_tag="ACHW42_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945540.1"
                     /translation="MKYDDVSELMIKREHNILIVGNSASHIDKFFKGYSIPGSKYYDF
                     TQINSDSDVERNEDAVSFIRDAMNSEEQTIIFNCAGWPDLGGGSAVSQFAMMVRKFGK
                     QLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHTKNELTGGKRRYRIKNGCK"
     gene            complement(2626..3216)
                     /locus_tag="ACHW42_003941"
     CDS             complement(2626..3216)
                     /locus_tag="ACHW42_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR8945541.1"
                     /translation="MKLIIFILIVLIIAALLIRIILKSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            complement(3254..3463)
                     /gene="hha"
                     /locus_tag="ACHW42_003942"
     CDS             complement(3254..3463)
                     /gene="hha"
                     /locus_tag="ACHW42_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR8945542.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(3509..>3757)
                     /locus_tag="ACHW42_003943"
     CDS             complement(3509..>3757)
                     /locus_tag="ACHW42_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR8945543.1"
                     /translation="AGKTVTVTYFQRDRYGRILGQVYAPDGMNVNQFMVRAGAAWVYE
                     QYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
BASE COUNT          933 a          991 c          811 g         1022 t
ORIGIN      
        1 tggcagtgaa gcgcccttcc gcgatttcgc gcgtcagctg ccgtttcacc agggtgacaa
       61 tatcctgacg ttccctgtcc gcatcacgac gcgcacgggc acgctttatt ccccgggact
      121 taagctctgt ctgataactg cgaaaacgct cacgaacaaa acgccaggct ttcgctatca
      181 gttcatccat gcccagggta tccagcccct gctttttgcg ttgtttgttt tcccatacca
      241 cacggctgcg gcgcgcggcg gccgctgcct cctctgatac atcgagggca gcaaacagtg
      301 cagatgtgaa cgtgatatcg gtcggaatgt agcacccgat aagcgggtca tattccgtct
      361 ggtaggtaat cagtcccagc tctgacagga acgtcagggc ccgggtggca cgggtgatgg
      421 agagttttcc ggcagcagac tccgtcgcca gtccgcactc aatggccagc gtggtgatgg
      481 agcactggac gcggttggcc agcgggtcat agtggaaaca cagcccctgc aggagcgcat
      541 caatagcccg tcgacgcagc accggtggca tgcgtcgacg cagaccacgc gaacgggcat
      601 gcgccacatg aatggcgaaa tcaaaacggg aagtgaagcc caccgccttt tccatcagtt
      661 tttcgcagaa cttcagcgtt ccggcacctt tacggggtgt aaacaccgga ttcgggttct
      721 ttacctggcg gtaatacgtt tgttgaagat cagtcacacc atcctgcact tacaatgcgc
      781 agaaggagtg agcacagaaa gaagtcttga acttttccgg gcatataact atactccccg
      841 catagctgaa ttgttggcta tacggttttt agatggcccc ggtaatcttc tcagtcgcca
      901 aacttgtcga agattatcgg ggttttcact tttctggctc ctgtaaatcc acatcagaac
      961 cagttccctg ccaccttacg gcgtggccag ccacaaaatt ccttaaacga tcagcaatct
     1021 atcacgcaca ccagaaacag gcaagaatgt gatgtcgcta cattttagtt agctaaaagc
     1081 actcttctgc tttatcagtt cggcaatcat ctcagcctgg gtaacgccct cagcttcaca
     1141 catcacctgc aattgcgctt ttaacgcagc aggaatgaac actctgagtt ctttatgtgt
     1201 agctcgtttt cttgccaggg atgcctgctg acgttctgca agcgtcagag gattaccttt
     1261 tctgtatgct cgctttgtac ctgatgagga agtcactgca ttttctgtct gcgacatctc
     1321 gcctcctcaa tactcaaaca gggatcgttt cgcagaggat actacagttt tttgaaatca
     1381 gcgacttgag aattgtgacg aagatctgtg ttggcgcaca aatcaacggg gattactgtt
     1441 gtttaatgtg atttttgttg cgaaatggct gaaaaaacag gaaatgcctt aacccgggag
     1501 ccggccccgg gcgggaccgg ctgtttctgg catgtcgggt gtctgcccct gaaatacctc
     1561 acacttccgg aaagcgggga cttccgtccc cgcccggcag tttacttccg tgcttcgtag
     1621 gccagagtta cctgcaccac tgtatttccc ctgtgaatat tcagctcaca taaccgttcc
     1681 ctgaatatca gtgaaaaaca caacaccgtg gcgcacacgg cgagcaaccc gataagggca
     1741 tacttcgtca taaactttga cctctctgca aggaagaggc tacaatcagt agtgcaatgt
     1801 acttcatgca gacctcacga gttaatggat taacaagtgg ggtctttgca tttctggtac
     1861 ctgcgtcttg cctgaaccag aaaggctcag acacccgccg cgataatacc acattccctg
     1921 ttgctgacta ttacctgcat tgcccgcttt ttactacaga agcacgaagg cgtcactcca
     1981 gccaataagt atgcttttta tttacatcca tttttaatcc ggtaccgacg ttttcctcca
     2041 gttaactcgt ttttcgtatg actcatggca ataaaatttt ctttaccata tgacaacatc
     2101 ccaataatat caaagttgtc ttttaatttg atggcatcct tttcacttac agcgacaata
     2161 agttgtttac caaattttct gaccatcatt gcaaactgtg atactgctga tccaccacct
     2221 aaatcaggcc acccagcaca attaaaaatg attgtctgct cttcactatt cattgcatcc
     2281 ctgataaagg agacagcatc ctcatttctc tctacatctg aatcactgtt aatctgagtg
     2341 aaatcataat atttagaacc tggtatagaa taccccttaa aaaacttatc aatgtgggag
     2401 gcagagttcc caacaataag aatattgtgt tcacgcttaa tcatcaattc tgatacatca
     2461 tcatatttca tatattcacc attattatat aaagtttggg tacaggtggt tccggaattt
     2521 actctgtgac caccatttcc cgctttttat tacagaaacg ccgggagcgg ggaaaacttg
     2581 ctttttcccg tttccggggt tggacaactg agcaacgcga aggcgtcagc tacgatgttc
     2641 cggggactgc tgatcagccc ctgttgtatt cagccattct ccggtcatct gcagcagttt
     2701 tctggcatcg tggcttctga gcaacggaat accagcctgc ctcagaacct cttccagaag
     2761 aatatcccgg cgccggcgtt ccggtcgtaa atggctggca tcatccagct ctactgctgc
     2821 aacaatactg aacgaacgcc gttcaacgat aaccacatca acatgccact gagacaccat
     2881 cctgaataac tgccaccact ggcgtgatcg tggccggata ttcccgttca gctggacgat
     2941 atccgcgacc cgtacctgcg gacacagata ccaccgcttc atatccacaa ttctgaacag
     3001 tcctttcata aaactgactt ccctttctgt cagcagcgtc ttctgacgtt gccagtattc
     3061 accgccggac agtattcgct ctgcatcacc ggtccgtata cctgccgcat gcaactgcat
     3121 cagcaaagga gaatgctgat ttacactttt gaggataatc cggataagta acgctgcaat
     3181 aatcagaaca atgagaataa aaataatcag cttcattgtt tatctgctcc tggtaattaa
     3241 ttattctgaa ttattacctt acatatttcc atatctcttt tggtatcctg tcatataact
     3301 ttccggtgat taattccgct aaccggtggt ctgctgctga attaaatgat tcacgttcac
     3361 tgtttaataa actgtctctg tttttatgaa taattttttc cagcgtattg actgatgaac
     3421 atcgccgtaa ctgatacagc cattcctgtt ttgttttcgc catttttatt cccgctgaga
     3481 acagatgaat gataagattg ccatcgcttt atttacggtg tctccatatc caggggggaa
     3541 ccggatcagc atctgaccag agcccacgtt tttgttgtct ggcttcgttc tgaaggacag
     3601 gcagcacggg atctgtgttg tactgctcat atacccaggc agccccggcc cggaccataa
     3661 actgattaac attcattccg tctggtgcat aaacctgccc gagaatacga ccataacgat
     3721 ctcgctgaaa atatgtgacg gtaaccgttt ttcctgc
//
LOCUS       DAZSNM010000280         3737 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZSNM010000280 DAZSNM010000000
VERSION     DAZSNM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.280"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            368..2020
                     /gene="ushA"
                     /locus_tag="ACHW42_003944"
     CDS             368..2020
                     /gene="ushA"
                     /locus_tag="ACHW42_003944"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
                     /protein_id="HFR8945544.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2031..2201)
                     /locus_tag="ACHW42_003945"
                     /pseudo
     CDS             complement(2031..2201)
                     /locus_tag="ACHW42_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2334..2453)
                     /locus_tag="ACHW42_003946"
                     /pseudo
     CDS             complement(2334..2453)
                     /locus_tag="ACHW42_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2527..3526)
                     /locus_tag="ACHW42_003947"
                     /pseudo
     CDS             complement(2527..3526)
                     /locus_tag="ACHW42_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1032 a          861 c          857 g          987 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggaacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg tatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggtcagtcc
     2341 tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt cagacggcat
     2401 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga accccgccgg
     2461 agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc gtcatacggc
     2521 tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct tcggatactg
     2581 tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt atttagactg
     2641 catccactta agcaaacgtg actgcattgc actccacagt cgatagctga agtttctgga
     2701 ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc gcctcgcaaa
     2761 atccagcaaa ctctcagctg ttgagcgatg tatacgccac ttcttgattg tttctgttat
     2821 tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt cgcctttgaa
     2881 attcttcaga gtacgcactt tgaagtcata tccgagagtg gcatacaaca tcatcggcgt
     2941 acctttcgaa gggaacgccg ggaaactgac gttgcatcca catatcaaag gcatagtgca
     3001 ggaacagatt tgagagcagg ggggagatta cgccgccctg cggcgttcct cgaccccgtg
     3061 ccagcattgc tccatccggc atttgcaccg gtgccttcag cctgcgctca atgtacatca
     3121 ccacccagcg tgcctgagta tgatgctgca ctgtcttcag tagcaggtca tgatcgatat
     3181 catcaacgaa gcctttaata tcgacttcca ccacccagtc aaacttccag cagcgtttcc
     3241 tcgcctgcgc aatcgcctgg tgtgcggatt tacccggtcg ataaccatac gagtctgcat
     3301 ggaacagggg ttccagtacc ggctcgagta tctgcttaac gaccatctgg gcgatccggt
     3361 ccgacacggc cggaataccg agcggacgta caccaccgtc agctttggga atgtccacac
     3421 gccttaccgc ctgcggcata taactgccgg aagccattct gttccacagt ttgtagagat
     3481 tgtctgtaac attctcgtca aacccggcca gtgtctgccc gtcaatgcct gccgccccac
     3541 ggttggcttt tactcgttta taagcttccc atacctgtcg tttacttaca ggaaatggct
     3601 ttgccgattt tacagcagtc ctcccatcgg cattgtcgcc cgtggttgcc gccgcaccat
     3661 cagcacaatg gggcaagccc tttgctccgg acccattacg gtccttcacc gctactacgg
     3721 tttactccgt ccctgta
//
LOCUS       DAZSNM010000281         3671 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZSNM010000281 DAZSNM010000000
VERSION     DAZSNM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.281"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..795
                     /locus_tag="ACHW42_003948"
     CDS             <1..795
                     /locus_tag="ACHW42_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR8945545.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="ACHW42_003949"
     CDS             806..1897
                     /locus_tag="ACHW42_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8945546.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHW42_003950"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHW42_003950"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR8945547.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..>3671
                     /gene="lpoA"
                     /locus_tag="ACHW42_003951"
     CDS             2865..>3671
                     /gene="lpoA"
                     /locus_tag="ACHW42_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR8945548.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQA"
BASE COUNT          937 a          945 c          906 g          883 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc a
//
LOCUS       DAZSNM010000282         3670 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZSNM010000282 DAZSNM010000000
VERSION     DAZSNM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.282"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..356)
                     /locus_tag="ACHW42_003952"
     CDS             complement(<1..356)
                     /locus_tag="ACHW42_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945549.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYG"
     gene            complement(618..2207)
                     /locus_tag="ACHW42_003953"
     CDS             complement(618..2207)
                     /locus_tag="ACHW42_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR8945550.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            2624..3622
                     /locus_tag="ACHW42_003954"
                     /pseudo
     CDS             2624..3622
                     /locus_tag="ACHW42_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
BASE COUNT         1066 a          709 c          646 g         1249 t
ORIGIN      
        1 caaccgtaat ttttactatt aattgtcaca ataaactgat aacagttatt cctacgtaga
       61 atatgagcgc gcatgcccga tagcatacta tatttttctg aatgattcgt ttgtatggta
      121 aatgaaattt tgtcatcttg aatagattgt tggaaacgtt ctatttgcca atcatcaatt
      181 ttttccacaa cttcaccgag cattgtcctg gcaatacttg atatatcatc acctatacca
      241 tttgcaatat acccaaacag ttctttagtg aagttggtaa catgctcttg caccatatca
      301 tttgttgcga ttgtcgttgt actgccatta gtgcctgcca cagttccaat tttcatcttt
      361 tacctcatat tattcaccgc gaggaataca tagttaacat tttatcttat gacatacacc
      421 tcatattcga tggggaaata attatatttt gcacaaatca ttcagcctta aagcataata
      481 tgaaatttct tatctcatag cagagaaagt ccctaaatat tgtagaggcg tcatcagtac
      541 atacctctac aatcgattaa gaaaacctat gaaatactgg caaaattaaa aagagatacc
      601 acagcactca tgaagtttta ttgaggttga aaacaactaa gccctgggac acctttttta
      661 tcaccacgtt cgttaggtgc tatcttccaa gaatttagcc ccatggtata attatcaatt
      721 ttttgatgct ggtctacccc taatccataa tggccaaatt tctgtttagt ttttttgagc
      781 gtctgtccac atttgaagat atcttcaggt tttggtctga aagcagtagt gttattaaaa
      841 taattttcat atacaggtgt gggtgctata gcattgcttc taattagatg gagaagatga
      901 tcatccatgt gttgtttatg ttttttatta catatatttg ctaattcctc aggatcaatt
      961 gggtctttca tctgattaaa tggaacctgc aaagcaccga gacaataaca ttgattttct
     1021 tcatttctta ttattccgtg atgactggtt aaaactttag tttctgagtt ataattacaa
     1081 acatcgagcg ttttaattaa gttatacgta tcaccggcag agagtttata attttctagt
     1141 cgtgcagtat agttaccatt aaaattgatt tcatgatttc cagccagaac gatgacattt
     1201 ttattaatac ggctatcttt attaccctcc atatttcgca tagaatttag caaagtaagg
     1261 atatatttat caccaaaaat agtactaaat ctatcgcctg tatgatcccc aaggaagata
     1321 caaggggtta tcgcatcgtc ctttaaaaaa acatgttgat gcaactcatt caatatcatg
     1381 tctctcgtat ttaataaatt gtagagcaat ttattatttt tatgtatgta tgcagatgaa
     1441 gccatgtctt cgtgttttaa taattcacat aataaactcc aacccttttc cctgatgcct
     1501 atatgccctg aaacgagcaa tgaataaagt acagcgccta ctcttccatc tgtatcgcca
     1561 aaataagccg tgttggcatt ttgtgggtta atcacaccac attgtttata aaagtctatt
     1621 ttagaaaaca cagatttaac atagctatca aacttatcct taggaaacgt ctctttagag
     1681 acatcccaac gccagtcagc tttaaaatta ccgctaagaa gagtaaagaa attttcaggt
     1741 atgatagaaa gcccattaac tttataaatt atgtctgatt tatctccgga gaaattaata
     1801 aaataataac catccttaca ctcaatctta actgtaatgg gagacgataa ttttatcaca
     1861 tggttattgt ctggtataat tgtaaagata aacgtgttag tagtattatc ggcaaaacta
     1921 ttatattctt gaatatctat agatggctca ctattgtaaa accatatttt ctccatctga
     1981 agactatgtt catttaccct ccagtgacca tgttgcagga aatagatgat atgcatgcca
     2041 aaatcgttat ctatttgcga atcattatcc agcttatcaa tttcaactat ctttgaacga
     2101 tgtgttttat caaccttgct atggtgttga tgattattaa ctttatctga atgcttatct
     2161 ttatggtttg aagggatgta cttaaatata ttgtcaattg ctttcataaa taacccgcga
     2221 tatttttgac ctgttagtaa aaaataaatg atacatgtct gaaagacata tttacgacga
     2281 tataaaacaa taatacttat atgttcgctg attttaagca ccgagactat ccggtctttt
     2341 actaaataat ggaaaacaat ccatccctgg aagacaattg taaaaaaaac aacagaataa
     2401 gaattaatct taggataaat atttatttat catggccttt tgaccgctaa aaaatggcac
     2461 tcggcacagt aaaccccgct atagttatgc tatgcatttg tctttaatga tatctcgtcg
     2521 ttatattaac aggctgaaat tatcattaat gcacatttca atattagcag ggatacctga
     2581 ggagtttaaa gtgaacatat atatcgggta gcttttcaaa ttaatccctt tgattatagg
     2641 cttaatttgc atcgcgttag gtggctttgt gctggaaagt tcagggcaac gtcacgggtc
     2701 atgtgctgat ttctctggcg gccatatgcc tggcattatt cactaccgca tttattatca
     2761 tttcgcaact cacgcgcggc gttaatacgt tttacaatac attgttcccc attattggct
     2821 atgcggggtc aattatcacc atgatatggg gttgggcact gttagcaggt aatgatgtga
     2881 tggcagacga gtttgttgcc ggccatgtta tttttggcgt tggtatgatt gccgcatgtg
     2941 tatcgacggt ggcagcgtcg tccggtcact ttctgctcat tcccaaaaat gcagcgggga
     3001 gcaagagcga cggaacaccg gtacaggctt attcttcatt aatcggtaat tgcctcattg
     3061 ccgttcccgt tttactcacc ctgctcggtt tcatttggtc tattacgctg ttacatagtg
     3121 ccgacataac tccgcattat gtcgccttgg gttaaccgca atctgtgcct gtctaattgg
     3181 cctagtggcc acaatcgtcc atcaaacacg taatacgttc tcatccaaag agcactggct
     3241 gtggtgttat tgggttattt ttctcggctc agtcacggtg ctgcagggga tatacgtctt
     3301 agtcagttcc gatgcaagcg cccgactggc tcccggcatt attctcattt gcctcggaat
     3361 gatctgttac agcatattct caaaagtctg gctactggca ctggtctgga gacgtacctg
     3421 ttcgttagcc aacagaatac cgatgatacc ggtatttacc tgtctgtttt gcttgttttt
     3481 gtcatcattc ctcgcggaaa tcgcacaaac tgagctggac tactttattc catctcgggt
     3541 tctggttgga ttaggcgcag tatgttttac attgttctct attgtttcaa tattagaggc
     3601 cggttctgcg aaaaaaaatt aaaatttaca taatcccagc gttattcata tcttaacgct
     3661 ggttttttac
//
LOCUS       DAZSNM010000283         3665 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZSNM010000283 DAZSNM010000000
VERSION     DAZSNM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.283"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            296..1549
                     /gene="kch"
                     /locus_tag="ACHW42_003955"
     CDS             296..1549
                     /gene="kch"
                     /locus_tag="ACHW42_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR8945551.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1604..1777)
                     /locus_tag="ACHW42_003956"
     CDS             complement(1604..1777)
                     /locus_tag="ACHW42_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR8945552.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1920..3380
                     /gene="cls"
                     /locus_tag="ACHW42_003957"
     CDS             1920..3380
                     /gene="cls"
                     /locus_tag="ACHW42_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR8945553.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3415..>3665
                     /locus_tag="ACHW42_003958"
     CDS             3415..>3665
                     /locus_tag="ACHW42_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR8945554.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDG"
BASE COUNT          910 a          837 c          872 g         1046 t
ORIGIN      
        1 agatccaata tttaagggca cggtttttgc aaccgtgttc ttgtttggcc tttccttata
       61 gccggtcagt gcattcacta ccacaagcag ataataactt tatctgtatt tctttatatg
      121 atgtgagcat gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt
      181 tgattactgc ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt
      241 tcagaaaatg tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag
      301 tcactgggct acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat
      361 tctcgcgctg gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc
      421 ggtcaatttg cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct
      481 ggcaaacgcc ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact
      541 gttccgggca aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta
      601 caccctgcat ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt
      661 gtttttgctg atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt
      721 tgcatttatt agtttcacga cattactgtt ttactcaacc tacggtgcgc tttatttaag
      781 cgaaggtttt aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac
      841 catgtcaacc gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac
      901 tatttcggtc attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg
      961 tccgcttatc cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg
     1021 taaagatcat tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact
     1081 gaatcaacgc ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca
     1141 acttgagcaa cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc
     1201 agtattaaag aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga
     1261 tgcagataac gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt
     1321 tctcgccgtc agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat
     1381 tctctcaccg caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat
     1441 taataatgat atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa
     1501 cgatgaacaa gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaaagac
     1561 ggaagagtaa acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg
     1621 caaacaataa cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca
     1681 tatttcggcg cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca
     1741 tgcgccagcg ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca
     1801 gaatggtcat tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg
     1861 ccagatgaat ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta
     1921 tgacaaccgt ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg
     1981 caggcgtaac tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc
     2041 tgttgattat ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc
     2101 tccatttagg caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat
     2161 ggcttaacga ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg
     2221 cgccattatt caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc
     2281 tacagctgat gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg
     2341 cgcgccataa tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg
     2401 tggctgaatc attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact
     2461 ccgccgggag tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta
     2521 ttgaagtggt cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc
     2581 tgcgccaaca tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga
     2641 atatggtcga tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga
     2701 tggcgcgtat ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg
     2761 agattgaaac cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg
     2821 aacaggccag cggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc
     2881 tcattcacca ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca
     2941 cgccctactt tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg
     3001 gggtggatgt cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca
     3061 gtcgcgcatt ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg
     3121 ggttactgca taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta
     3181 accttgatat gcgtagtctg tggctgaatt tcgagattac cttggcaatc gacgataaag
     3241 gttttggtgc agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg
     3301 atgcccgttt atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact
     3361 tcttcagccc gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat
     3421 atggatctaa acaatcgcct gactgaagat gaaacgcttg agcaggccta cgatattttt
     3481 ctcgaactgg ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt
     3541 gaagagcgcg gcggcgcgga gttattcgat ccggcggaag actggcagga acatgttgat
     3601 ttcgatttga acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat
     3661 ggtga
//
LOCUS       DAZSNM010000284         3663 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZSNM010000284 DAZSNM010000000
VERSION     DAZSNM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.284"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            139..1185
                     /gene="ansB"
                     /locus_tag="ACHW42_003959"
     CDS             139..1185
                     /gene="ansB"
                     /locus_tag="ACHW42_003959"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR8945555.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            1302..2264
                     /locus_tag="ACHW42_003960"
     CDS             1302..2264
                     /locus_tag="ACHW42_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR8945556.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(2365..>3663)
                     /locus_tag="ACHW42_003961"
     CDS             complement(2365..>3663)
                     /locus_tag="ACHW42_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFR8945557.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         1129 a          852 c          871 g          811 t
ORIGIN      
        1 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
       61 ttgtaaattg tttaacgtca aatttcccat gcaaagctaa gggataatgc gtagcgtcca
      121 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
      181 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
      241 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
      301 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
      361 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
      421 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
      481 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
      541 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
      601 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
      661 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
      721 tctgttaact acggtcctct gggatacatt cacaacggta agattgatta ccaacgtacc
      781 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
      841 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
      901 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
      961 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
     1021 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
     1081 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
     1141 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
     1201 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat
     1261 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
     1321 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
     1381 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
     1441 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacctggtca gcggaaggcg
     1501 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
     1561 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
     1621 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
     1681 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
     1741 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
     1801 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
     1861 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
     1921 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
     1981 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
     2041 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
     2101 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
     2161 aaaaagggca atatccgtta acgatgaaag tttcagaaat aaacagtaaa aaagtcgcga
     2221 ttttgatgaa aattgacgat atcaaccaag ctaatgaacg ctagaaaaaa gataccgaac
     2281 aactgcggcg caatggggtg attaagtaac tcatgttaag tgcctttgcc ggataatata
     2341 aaaagttatc cggcaaatta ttttttacat tagccctagt agctgtggaa tgaacaagga
     2401 tatttgcgga atgtaagtga tcatcatcaa cgctactagc aatgcagcat aaaatagcag
     2461 caatggtttg attaaatgtt gaatcttcac acccgagata gaacaaccaa caaatagcgc
     2521 actacccacc ggcggtgtta acagcccaat acaaaggttg gccaccatca taataccgaa
     2581 atgtactggg tccattccca gctcttgtgc aatcggcaag aagatcggtg taaagattag
     2641 tacagccggt gtcatgtcca taaagatgcc gacaattaat aagacgatat taatcagcag
     2701 caatatgatc agtgggttat cagaaatccc catcaatgcg tcgctgatca tataaggaat
     2761 atcagcatta gtcattgccc aggacatgcc cacggaaaaa ccgatcaaca gtaatacaat
     2821 cgaagtggta acaaccgact ccagaatcag tttgggtaaa tggcgccatt ttacttcccg
     2881 gtaaatcagc accgagagaa taaatgtgta gacaacggca attgctgacg cttcagttgc
     2941 ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac accagaagca aacttggtaa
     3001 ggcatccaga aacgctttac aggcctgagc aaaggttgct ctttctgaaa gcgggtagcc
     3061 acggcgttta gcaataatgc cgcagactat cattaccgca agacccatta agatccctgg
     3121 caaatatccc gccataaaca gcgatgctac agaaacgccg ccagctgtca gggaaaacac
     3181 aattaacacg tttgagggcg gtatcagtaa tccagtaata caagatgaga cgtttacagc
     3241 ggtagaaaat gccggatcat aaccttcttt ggtctgaatg ggattcagtg tgccaccaac
     3301 ggcagcagct gccgcaacgg ctgaaccaga aatggaacca aacatcatat tcgccaggac
     3361 gttcacatga ccaagcgaac caggaacgcg gccaaccatt acctgggcga ggttaatcaa
     3421 acgtatcgca ataccgccgc tgttcatcag tgttccggca aagatgaaaa aggggattgc
     3481 caacagggca aagttatcca gtccattagc cagtcgttgc gaaacagtga ttgccgcgat
     3541 atcccaggga aacatcaaaa gcattgaagc tacagtggca ataccgattg agaacgaaat
     3601 gggcacacca ataaagacca gcacaaaaaa acagccaaac agtgtaatcg cgatataact
     3661 atc
//
LOCUS       DAZSNM010000285         3589 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZSNM010000285 DAZSNM010000000
VERSION     DAZSNM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.285"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..490)
                     /gene="yeiL"
                     /locus_tag="ACHW42_003962"
     CDS             complement(<1..490)
                     /gene="yeiL"
                     /locus_tag="ACHW42_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFR8945558.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQ"
     gene            621..1562
                     /gene="rihB"
                     /locus_tag="ACHW42_003963"
     CDS             621..1562
                     /gene="rihB"
                     /locus_tag="ACHW42_003963"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR8945559.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            1662..2912
                     /locus_tag="ACHW42_003964"
     CDS             1662..2912
                     /locus_tag="ACHW42_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR8945560.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2968..>3589)
                     /locus_tag="ACHW42_003965"
     CDS             complement(2968..>3589)
                     /locus_tag="ACHW42_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR8945561.1"
                     /translation="DDQTVLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAE
                     ALEWVFTLADEIPVFVDTVSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADR
                     NAAVNALHQQGVQQLFVYLPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLV
                     YSFLEGNNFRDSARFAMACAAISRASGSLNNPTLSADNALSLVPMV"
BASE COUNT          883 a          849 c          928 g          929 t
ORIGIN      
        1 cttgtgagag taatataaat gctgccaggc gattcactaa cggaaatgat tgattctgag
       61 ttaaagaaac aatattacga taatttttat gacttaaggt gacgcaaagt tttcgtaaaa
      121 atagggtgtc gtttaataac agcggacggt aatgtttcat agggagcgca aggcaccaac
      181 actcttcaat agcctgcacc gcacgcggtt catggtcttt gtcgattaac tcaatctcgc
      241 caataaaaca gggagcggca aagaaatcga tcagcgacac gcgaccattg gcaagcgtgg
      301 cgtaaagcct ggcgcggcct cgcgtcagat aaaacagcca tgacggctgt tgcccttcct
      361 gcacaatgta gtcacgcgct aaaaaatgaa acagccgcgt atcggctgaa acatccgttg
      421 aaaagcaatc cttaaacgca gattcactca tcaattgctg cttaaggtca ttattgtgga
      481 tttctttcac gatgtttatc cgctatgaga tttctcatat ttagcatacg tacgctgttg
      541 ttaagattca atcattggcc cggtcgaatt ttctttgctg tagaaaaaaa taacgcaact
      601 ggaaacagag gaaataaaca atggaaaaga gaaaaattat tctggattgt gagccgggtc
      661 atgatgatgc tattgcaatg atgatggcag cgaaacatcc ggcaatagat ttattaggca
      721 tcactattgt agcgggtaat cagacgcttg ataaaacatt aattaatggc ctgaatgttt
      781 gccagaaact ggagattaat gttccggttt atgcggggat gccgcaaccc attatgcgta
      841 aacaaatcgt tgccgataat attcacggtg aaaccggact ggatggcccg gtattcgagc
      901 cgctgacccg ccaggcagaa agtacccatg cggtgaaata tatcatcgat accctgatgg
      961 caagcgatgg cgatatcact ctggtgccgg ttggtccgct ttcaaatatc gcggtggcaa
     1021 tgcgtatgca acccgcaatc ctgcccaaaa tccgtgaaat tgtgctaatg ggcggcgctt
     1081 acggtacagg caacttcacc ccatctgccg agttcaatat ctttgccgac ccggaagccg
     1141 cccgcgtggt gttcacctcc ggcgttccat tagtgatgat gggcctcgat cttaccaacc
     1201 agaccgtttg caccccggat gtaattgctc ggatggaaag ggtaggcggc cctgccggag
     1261 agctgttcag cgacatcatg aacttcactc tcaaaacgca gttcgaatac tacggcctgg
     1321 ctggcggccc ggtgcatgac gccacctgca ttggttatct gatcaaccct gatggcatta
     1381 aaacccagga tatgtacgtc gaagtggacg tcaacagcgg cccttgctac gggcgtacgg
     1441 tctgcgacga actgggcgtt cttggcaagc ccgctaatac caaagtcggc atcactattg
     1501 ataccgactg gttctggggc ttagtcgaag agtgcgtgcg cggctacatc aaaacccatt
     1561 aagtgttgat aacaagccgg gacagtatcg caagatgcag gcccggctat ttctgatggg
     1621 cagaaagccg tcgcccaaaa tgacatgaag agataatcac tatggatgtc atgagaagtg
     1681 ttctgggaat ggtggtactg ctggcaattg cgtttttact gtcagtaaac aagaagaaga
     1741 tcagcctgcg taccgttggc gcggcgttag tgttacaggt cgtgattggc gggattatgc
     1801 tttggttacc gccagggcgt tgggtcgcag aaaaagtcgc ttttggcgtc cacaaagtga
     1861 tggcgtacag cgacgcaggt agcgcattta tcttcggttc tctggtcgga ccgaaaatgg
     1921 ataccttatt tgatggcgca ggatttatct ttggtttcag ggtattaccg gcaattatct
     1981 tcgtcactgc actggtgagt attctctact acatcggtgt gatggggatc ttaattcgca
     2041 ttctcggcgg tatcttccag aaagctttaa atatcagcaa gatcgagtca ttcgtcgcgg
     2101 tcaccaccat tttcctcggg caaaacgaaa ttccggcgat cgtcaaaccc tttatcgatc
     2161 gtctgaatcg caatgaatta tttacagcga tttgtagtgg catggcctcg attgctggtt
     2221 cgacaatgat tggttacgcc gcactgggcg tacctgtgga atatttgctg gcggcatcgt
     2281 taatggcgat ccctggcggg atcttgtttg cccgcctgtt aagcccggct acggaatctt
     2341 cgcaggtttc ttttaataac ctctctttca ccgaaacacc gccaaaaagc attattgaag
     2401 ccgatgcgac aggggcaatg accgggctga aaatcgccgc cggtgtagcg acagttgtta
     2461 tggcatttgt cgccatcatt gcgttaatta acggtattat cggcggcgtt ggcggctggt
     2521 ttggttttga acatgcctcg ctggagtcca ttttaggtta cctgctggcc ccacttgcgt
     2581 gggtaatggg tgttgactgg agtgatgcaa atcttgccgg gagtttgatt ggacagaaac
     2641 tggcaataaa tgaatttgtc gcttatctca atttctcacc ctatctgcaa acggctggca
     2701 ctctggatgc taaaaccgtg gcgattattt ccttcgcgtt gtgcggtttc gctaactttg
     2761 gttctatcgg ggtggtggtg ggggcgtttt ctgcggttgc gccacaccgt gcgccggaaa
     2821 tcgcccagct tggtttacgc gcgctggcgg cggcgacact ttctaatctg atgagtgcca
     2881 ccattgccgg gttctttatt ggtttagcgt agtgtgcaat ttatgagccg gattagatgc
     2941 ggtcaagcgt cgaatccggc aacattgtta caccattggc actaatgaca gcgcgttatc
     3001 ggcagacagg gtagggttgt ttaagctgcc gctggcgcgt gaaattgccg cgcaggccat
     3061 cgcaaaacgg gcgctgtcac ggaaattgtt tccctccaga aagctatata ccagacccgc
     3121 cataaaacca tcgtcagcac caaaactgtc taccgtcgta tgcgctggca cagtcagcaa
     3181 aaattgttca ccatcctttt cgctgcaata aactgactca tcgggcaaat aaacaaacag
     3241 ttgctgaaca ccttgctgat gcaatgcatt cactgcggca ttacggtcag catcgctggt
     3301 gatcgcctgt ccccataaaa tttccagctc cggtaaagtg ggtttcaggg tgtgaatatg
     3361 cgccagccag tgtttgattt tgcccgcttt gaattctgaa acggtatcga caaacaccgg
     3421 gatctcatca gcgagggtaa agacccattc cagcgcctcg gctgtcaggt tgcaatcagc
     3481 cagtaccacg cccgcatgac gaagtaaatc gcgcgacccg ttcagtagtt gcggtgtcaa
     3541 ctgttccagc agatgggtat cgttaattgc cagcacggtt tgatcgtct
//
LOCUS       DAZSNM010000286         3533 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZSNM010000286 DAZSNM010000000
VERSION     DAZSNM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.286"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..827)
                     /locus_tag="ACHW42_003966"
     CDS             complement(<1..827)
                     /locus_tag="ACHW42_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFR8945562.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTF"
     gene            950..1396
                     /locus_tag="ACHW42_003967"
     CDS             950..1396
                     /locus_tag="ACHW42_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR8945563.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            1406..>3533
                     /gene="yccS"
                     /locus_tag="ACHW42_003968"
     CDS             1406..>3533
                     /gene="yccS"
                     /locus_tag="ACHW42_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR8945564.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
BASE COUNT          777 a          939 c          886 g          931 t
ORIGIN      
        1 tgaaacgtgc gtgcggtaat gtcttcggta tgtagccgtt cgcgaatccg ctcgtccatc
       61 tcttcagcgg ctttgcgacc aaacgccagc aataaaattt gctcagggga cgcttcacca
      121 cgcgccagca accagcctgc acgggccacc agcaccgacg tttttccgct tcctgcacca
      181 gccagcacta acagagaatg ctcgccatta acgactgccc gggcctgcgc cggattcagc
      241 ggtgaagact cgacctggcg gaaaaaatcc gcatactcgg taagcatggc ttcggtatac
      301 gcctggttat gctgcaaccg agcgctttca atatctttca accaagcctg acatttacgc
      361 cacgcctcac ggcagttatc gaattcttcc agtcggttaa ccggcaacgg caacgccgac
      421 aaagcctggc ggatttgttg ctgaacgcca gaggtttgct cacgcgtcag ccatttattt
      481 tctccagtgc gcgtggcaat caaatccagt tgctggcgta aaacaccaga cgcaatttcg
      541 ctcatctcgc cactccaccg ccgccagtga gcatcaagat gatggtaaaa acgctgcgtc
      601 tcgccccatt ctgtgccgtg cagacgcacc actttttcgt ccggcagtac aaattccagc
      661 tcgccccata ccagaccgcg cttacagtga atcgccagta attgattgaa aggaataagg
      721 tattcgtggc gatcgccgga gactttaatc ccggcattga ggatcaccgc ccgatcgtaa
      781 gggtgctgtg ccagacgttt tccaagcgtt gtcgctttca gttccatgac tctgtagatc
      841 cacacgaaat tatttcttat cagtgtaacc gtcagagaaa acgtgctcca gcccaaaaaa
      901 acgttacaat tgccgcccat tactgaaaac cacagtaaag cgaggtttta tgcgtaccgt
      961 tttgaacatt ctgaactttg tgcttggcgg atttgccacc actctgggct ggctgttggc
     1021 gactctggtc agtattgtgc tgatttttac cttaccgctg acacgatcct gctgggagat
     1081 cactaaactg tctctggtgc cttatggcaa tgaagctatt catgtcgatg aactgaaccc
     1141 ggctggcaaa aatgtgttgc tgaatactgg cggtacggta ttgaatattt tctggctgat
     1201 tttctttggc tggtggttat gcctgatgca cattgcaacg ggcatcgcac aatgtatttc
     1261 aatcattggc attcctgtcg gcattgcgaa ctttaaaatt gccgctattg cactatggcc
     1321 ggttggtcgt cgcgtggtat cggtagaaac agcgcaagct gcgcgtgaag ccaatgcacg
     1381 tcgtcgtttt gaataatcca ctaatatggc ctttatgcta agtcctttgc tcaaacgcta
     1441 tacctggaac agcgcctggc tgtattacgc gcgtattttt attgcgcttt gtggaaccac
     1501 agcgtttccg tggtggctgg gtgatgtaaa actgacgatt ccgctaacgc tggggatggt
     1561 ggcagcggcg ctgaccgatc tcgatgaccg actggcggga cgtttgcgta acctcatcat
     1621 tacgctgttc tgctttttta tcgcctcggc ctcagtagaa ttgctgtttc cctggccctg
     1681 gctatttgcg attggcttaa cgctctctac cagcggcttc attttgctcg gcggtctggg
     1741 tcaacgctat gcaacaattg ccttcggtgc attgctgatc gccatttaca ctatgttggg
     1801 aacatcactg tatgagcact ggtatcagca gccgatgtat ctgctggccg gtgccgtctg
     1861 gtacaacgtc ctgacactta ttggtcatct gctgttcccg gtccgcccgc tgcaggacaa
     1921 cctggcgcgt tgctatgaac aactggcgcg ttatcttgag ctcaagtcgc gcatgtttga
     1981 tcctgatatt gaagatgaaa gccaggcacc gctgtacgat ttggctctcg ccaacggtca
     2041 gctgatggcg acattgaatc agacgaaact ctcgctgctg acccgcttac gtggcgatcg
     2101 tggtcaacgg ggaacgcgtc gcacgctgca ttattacttt gtcgcacagg atattcacga
     2161 gcgtgccagc tcttctcata ttcagtatca aacattgcgt gaacattttc gccacagcga
     2221 cgtgctgttc cgttttcagc ggctgatgtc gatgcagggc caggcgtgcc agcaactgtc
     2281 acgctgtatt ttgttgcgtc agtcttatca acatgatccg cattttgagc gcgcttttac
     2341 gcatattgat gctgcgctgg agcggatacg cgataacggc gcacccgccg atttactcaa
     2401 aacactggga tttttgctga acaatttacg cgccattgat gcccaactgg caacaattga
     2461 atcagaacag gcccagacac taccccgtaa taatgacgaa aatgagctcg ctgatgacag
     2521 cccgcacgga ttgagtgata tctggctgcg tcttagccgt cacttcacgc cggaatccgc
     2581 cctcttccgt catgcggtaa gaatgtcgct ggtgttgtgc ttcggctacg ccatcattca
     2641 aataaccgga atgcatcacg ggtattggat cttgctgaca agtttgtttg tctgccagcc
     2701 aaactataac gccacgcgcc accgcctgaa gttaaggatt attggtacgc tggtaggtat
     2761 cgcgattggc attcctgtgc tgtggtttgt gccatcactg gaagggcagc tggtgctgct
     2821 ggttattacc ggcgtgctct tttttgcctt ccgtaacgtg caatacgctc atgcaacgat
     2881 gttcatcaca cttttggtgc tactgtgttt taacttactg ggtgaaggtt ttgaagtagc
     2941 gttacctcgc gtaatcgata cgctgattgg ttgtgccatt gcgtgggcgg cagtgagcta
     3001 catctggcct gactggaagt ttcgcaatct gccgcgaatg ctcgaacgcg ccacagaggc
     3061 caactgtcgc tatctcgatg ccatactgga gcaataccat caggggcgtg ataaccgtct
     3121 ggcgtatcgt attgcccgcc gcgatgcaca caaccgtgat gctgaactgg cgtcggtggt
     3181 atcaaatatg tccagcgaac cgaacgttac cccgcaaatt cgcgaagccg cgtttcgctt
     3241 gctgtgcctt aaccatacgt ttaccagcta tatctcagcc ctcggtgctc accgggagca
     3301 gttaactaat cctgaaattc tggcgtttct tgatgacgca gtttgctatg ttgatgacgc
     3361 gttacatcat caacctgctg atgaagaacg cgtcaatcag gcattagctg gcctgaaaca
     3421 gcggatgcag caacttgaac cacgggcaga tagcaaagaa cctctggtcg tacaacaagt
     3481 tgggttattg attgcattac tgccagagat tggtcgtctg caacgccaga tta
//
LOCUS       DAZSNM010000287         3493 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZSNM010000287 DAZSNM010000000
VERSION     DAZSNM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.287"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..311
                     /locus_tag="ACHW42_003969"
     CDS             <1..311
                     /locus_tag="ACHW42_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFR8945565.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="ACHW42_003970"
     CDS             468..1217
                     /locus_tag="ACHW42_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR8945566.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="ACHW42_003971"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="ACHW42_003971"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR8945567.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACHW42_003972"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACHW42_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR8945568.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..>3493
                     /locus_tag="ACHW42_003973"
     CDS             3361..>3493
                     /locus_tag="ACHW42_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR8945569.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     "
BASE COUNT          961 a          908 c          863 g          761 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aac
//
LOCUS       DAZSNM010000288         3430 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZSNM010000288 DAZSNM010000000
VERSION     DAZSNM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.288"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..453
                     /locus_tag="ACHW42_003974"
                     /pseudo
     CDS             1..453
                     /locus_tag="ACHW42_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            513..620
                     /locus_tag="ACHW42_003975"
     CDS             513..620
                     /locus_tag="ACHW42_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR8945570.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(601..1332)
                     /gene="yehW"
                     /locus_tag="ACHW42_003976"
     CDS             complement(601..1332)
                     /gene="yehW"
                     /locus_tag="ACHW42_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR8945571.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1337..2263)
                     /gene="yehX"
                     /locus_tag="ACHW42_003977"
     CDS             complement(1337..2263)
                     /gene="yehX"
                     /locus_tag="ACHW42_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR8945572.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(2256..3413)
                     /gene="yehY"
                     /locus_tag="ACHW42_003978"
     CDS             complement(2256..3413)
                     /gene="yehY"
                     /locus_tag="ACHW42_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR8945573.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
BASE COUNT          866 a         1059 c          855 g          650 t
ORIGIN      
        1 gcaatctaca tagcctgcga acgtggatca aagagcgcgg tcaggattac cccgcccaga
       61 cactcaccac acatctgttt attcctctgc gccgacggct tcagtgccaa caaccgactc
      121 tccaggcgct gctggcgatc ctcgacggcg tactgatcaa ctacatcgcc atttgtctgg
      181 cttcggcacg taaaaaacag ggtaaagatg cgctggtggt tggctggaat attcaggata
      241 ccacccgtct gtggctggag ggctggattg ccagtcaaca aggatggcgc attgatgtcc
      301 tcgctcgccc actcgctcaa tcaactacgc cctgaactat tcgaaggccg tacattgctg
      361 gtgtggtgcg gtgaaaatcg aacctccgcc caacagcagc aactcaccag ttggcaagaa
      421 caaggccatg atattttccc actcggtatt taatgattcg ttaacaaatg cgctttactg
      481 tacaatcctt tcgttaacat aaggagtgca ttatgcgcat agctaaaatt ggggtcatcg
      541 ccctgttcct gtttatggcg ttaggcggaa ttggtggcgt catgctcgca ggttatacct
      601 ttattttgcg tgctggctaa gcgcctgcac cagcctttca aacaggcggt ctgcgatgat
      661 cgccgccagt gccaccagta acgccccctg gatcacatac gcggtattaa atccgctaag
      721 cccgatgatg atgggcgtac ccagcgtact ggcccctacc gttgaggcga tcgtcgccgt
      781 accaatgttg ataatcaccg aagttcgcac gcccgccaga atcaccggag ccgccagcgg
      841 tagctcgacc ttacgcagtc gctgacctcg actcattccc atacctttcg caacttctgt
      901 cacgctggga tcaatcgctc ccagcccggc aagtgtcacc tgcaggatgg gcagcacacc
      961 gtaaaggatc aaggcgataa tcgctggttg cagaccaaag ccgatcaccg gaacggcaat
     1021 cgccagcact gcgacaggcg gaaaagtctg tccaacggcg gcaatagttt ccaccagtgg
     1081 gcgaaattcc gcgccccacg ggcgagtaac agcaattccg gcacctgtgc caatgatcac
     1141 cgcaaacaaa ctcgaaattc ccaccagcca gaaatgagcc agtgccagag ctgcaaaact
     1201 ttcttgctga taaacgggtc gtggcagttg tgggaacaag gcagcaaaca gcggctggct
     1261 gtaaggcagc cagaaaatca gcgccacaaa aagagcaatg agccagaaca gcggatcgcg
     1321 caacatcttc atacgcttac gcctccacca gcagatcctg aaaatgcagc gtgccgcaag
     1381 gctggccctg cgtgttcacc accggcagca cctcgcatcc ccgcgcgaca aacagggaga
     1441 gcgcatcgcg tagcgtcatc tcttctgcca gtgcctcacc ttctgcccgt tcttcgcgac
     1501 gcacgtaatc cgccacgcta cgtaacgaaa gcaggtgcac ccccagttca ctacgtccaa
     1561 aaaactggcg gacaaaatca ttcgccggac gagtcagcat cgtcagcgga ttcccctgct
     1621 gcaccacttc accgtgatcc atcaatacca gatgttctgc cagccgtagc gcctcatcaa
     1681 tatcatgagt gaccagcaca atagtgcgcc ccagcaaacg gtgaatgcgc gtcatctctt
     1741 gttgcaacgc gccgcgcgtt accgggtcca gtgcgccaaa aggttcatcc atcagtaaga
     1801 cttgcggatc ggcagccagt gcacgcgcca ctcccacacg ttgctgctga ccaccggaaa
     1861 gctgatgcgg ataacgctca cgcaaatttg actccagccc cagtagcgcc attaattcgt
     1921 cgatacgatc gtcaatccgc gcccgcgacc atttttgtaa ttgcggcacg gtagcgatgt
     1981 tttgcgccac gctccagtgg gggaacaggc caatagattg aatggcatag cccatccggc
     2041 ggcgcaactc cagtactggc agcgagcgaa tttcttctcc ggcaaagcgg atcactccgc
     2101 tgtcatgctc caccaggcgg ttaatcattt tcagggtggt ggatttgccg gagccagatg
     2161 tgccaatcag caccgaaaaa ctcccttcct gaaaattgag attgagatcg ttaacggctt
     2221 tttgtgcgcc gaacagtttg ctgacatggc taaattcaat cattacgttt caccttcagc
     2281 agtgcgataa gcaaatcgaa cagcgcgtcg gtaagcaccg ccagaacaat taccgggatc
     2341 acccccagca acactaaatc aatggcgctg cttagcagcc cctggaaaac cagcgcacca
     2401 aaaccgcctg cgccgattaa cgccgcaatc accgccatac ctacagtttg caccatcacc
     2461 acccgcaggc tgcgcagaaa taccggtaac gccagcggta actgaacatg caggaaacgc
     2521 tgcgccccgc tcatccccat cgctctggcg ctctccagca catcgcgcgg gatctggttc
     2581 aaaccgacta ccacgcctcg caccagcggc agcaaggcat agagcaccag cgcaatcagt
     2641 gcgggtgtca ttccggttcc tgctatgccg agcttcccca gccacggaaa ggccgtaacc
     2701 agcgcggcaa gcggcgcaat caacaggcca aagagcgcca ccgaaggcac ggtctgaatg
     2761 acattgagca gagaaaaaat tgccccctgc cgcgcagtgg aaaagtagca ccagatgccc
     2821 aacggcacac caatcactaa cgcaggcagc accgcaccaa acagcaacgt cagatgttgt
     2881 gccagcgcgt cgtcaaacac atcctgacgg ttggcgtatt ctttcattag tgagagatcg
     2941 ttaagcgtgc cggagtacag caaccacagc ggaataatgg caatctgcat atgcaacaac
     3001 cagcgccaca gcggatgcgt ggagattcgg cggatggcat cgctacaggc cagcaatgcc
     3061 agcgccgcag ccagccagaa acctctgccg aggctggtac gcgccagcgc actgccattt
     3121 tgcgccagtt gggtcgccgc ctttccagct ccccacacca acaatacgaa gacgaattgc
     3181 gccagaatga gtgcaaaaat gctgcctttt ttagcgggga caaaacaggc tgtcagccag
     3241 gcgcaaccaa cgcccaccag catccagatc gtttgcggcc acagttgcca gagatgaagc
     3301 ccttcacccg agactaaacg attaggcgcg taactgataa acggcagcgc cgctgcgatt
     3361 gccgtcagca acaacagcag cgccagaaca ggattaatac gtagataagt cacgggaaat
     3421 tacttcgccc
//
LOCUS       DAZSNM010000289         3424 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZSNM010000289 DAZSNM010000000
VERSION     DAZSNM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.289"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            87..803
                     /gene="nanC"
                     /locus_tag="ACHW42_003979"
     CDS             87..803
                     /gene="nanC"
                     /locus_tag="ACHW42_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR8945574.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            823..1929
                     /gene="nanM"
                     /locus_tag="ACHW42_003980"
     CDS             823..1929
                     /gene="nanM"
                     /locus_tag="ACHW42_003980"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR8945575.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1994..2974
                     /locus_tag="ACHW42_003981"
     CDS             1994..2974
                     /locus_tag="ACHW42_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR8945576.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCRMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3084..3289
                     /locus_tag="ACHW42_003982"
                     /pseudo
     CDS             3084..3289
                     /locus_tag="ACHW42_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         1002 a          727 c          802 g          893 t
ORIGIN      
        1 tggcaaaaaa atattacaca acataaatgc taattgttta tgcgggcttt gtattgcttt
       61 ctgtatccta caaatgagtg aaatttatga aaaaggctaa aatactttct ggcgtattat
      121 tactgtgctt ttcgtcccca ttaatttctc aggctgcgac actggacgta cgtggtggat
      181 atcgtagtgg aagccacgcc tatgagactc gactcaaagt cagtgaggga tggcaaaatg
      241 gatggtgggc aagcatggaa agtaatacct ggaataccat tcatgataat aaaaaggaaa
      301 atgccgcact caatgatgtt caggttgaag ttaattacgc aattaaactt gatgatcaat
      361 ggacggtgcg cccgggaatg ttaacgcatt ttagcagcaa cggcacacgc tacggaccct
      421 acgtaaaact gtcctgggac gcgacaaaag atcttaattt tggcattcgc tatcgttacg
      481 actggaaagc ttaccgacaa caagacttat ccggtgatat gtctcgtgat aacgttcatc
      541 gttgggatgg atatgtcact taccatatta atagtgattt caccttcgca tggcaaacga
      601 cgctatacag caaacagaat gattatcgct atgcaaacca caagaaatgg gcgacggaaa
      661 atgcctttgt tctacaatac catatgacgc ccgatattac gccatacata gaatatgact
      721 accttgaccg ccagggtgtt tacaacggca gagataattt atcggaaaac agttatcgca
      781 ttggtgtgtc atttaaactg tagtagacag gagacagtca caatgaataa aactataatg
      841 gcgcttgcta tcatgatggc ttcatttgcc gcaaacgcgt ctgtattacc ggaaactcct
      901 gtgccattta aaagtggtac cggagcaatt gataacgaca ctgtctacat tggtttaggt
      961 agcgcaggta cagcatggta caagctagat acacaggcca aagataaaaa atggactgcg
     1021 ttagctgcat tccctggtgg accgagagag caagcaacct ctgcatttat tgatggcaat
     1081 ctgtatgtgt ttggcggcat tggcaaaaac agcgagggat tgactcaggt atttaatgac
     1141 gtacacaaat acaaccccaa aaccaatagc tgggttaaat tgatgtcgca cgcgccgatg
     1201 ggcatggcgg gtcatgtgac ttttgtacac aacggcaagg cttatgttac tggcggtgtt
     1261 aaccagaata tcttcaatgg ctattttgaa gatctcaacg aggctggaaa agattcaacc
     1321 gctatagata aaatcaacgc ccactatttt gacaaaaaag cagaagatta tttcttcaat
     1381 aagtttctgt tgtcttttga tccctcaaca cagcaatgga gttacgctgg cgaatcgccc
     1441 tggtacggaa cggctggtgc ggcggttgtg aataaaggtg ataaaacctg gcttattaat
     1501 ggcgaagcca aaccaggatt gcgaacggat gccgtatttg aacttgattt caccggtaat
     1561 aatttaaaat ggaataagct tgatcccgtc tcatcaccag atggcgtcgc tggcggtttt
     1621 gcggggataa gcaatgattc tcttatattt gctggagggg ccggattcaa aggttcacga
     1681 gaaaattacc agaacggtaa gaactatgcg catgaaggcc tgaaaaaatc atatagcact
     1741 gatattcatc tttggcataa cgggaaatgg gataaatcgg gtgaattatc gcaaggtcgg
     1801 gcctacggag tatcattgcc ctggaataat agtttattaa ttattggcgg tgaaactgca
     1861 ggcggcaaag cggtgacgga ttcagtttta atttctgtga aggataataa agtcacagta
     1921 caaaactaac gcttcagggc cccggtaaag gggccctgct atcaatttgt catttgtatt
     1981 acgcgaaaat tatatgaacg caataatatc acccgattat tactatgttc ttaccgttgc
     2041 tggtcagtct aatgccatgg cgtatggcga aggactgcca ttaccggaca gggaagatgc
     2101 gcctcattcc agaattaaac aattagcgag atttgcgcat acgcatcccg gaggcccgcc
     2161 atgtcacttt aacgacatta ttccactgac tcactgccca cacgatgttc aggatatgca
     2221 gggttatcac catcctctgg caacgaatca tcaaacacag tacggcaccg ttggccaggc
     2281 actgcatatt gcacggaaat tactgccctt cattcctgat aatgcaggga ttcttatcgt
     2341 tccgtgttgc cgtggcggat cggcttttac cgcgggcagc gaagggacat attcagaacg
     2401 gcacggagcc agccatgatg cttgtcgttg gggaacggat actccgctgt accaggattt
     2461 agtcagcaga acgcgagccg cactggcaaa aaatccgcag aacaaattcc tcggcgtatg
     2521 ccggatgcaa ggcgaatttg acttaatgac cagtgactac gcgtcacacc ctcaacactt
     2581 taatcatatg gttgaagcct ttcgtaggga tctaaaacaa taccattctc agcttaataa
     2641 tattactgac gcgccgtggt tttgcggcga taccacctgg tactggaaag aaaatttccc
     2701 gcattcgtat gaagctattt atggcaatta tcaaaataat gttttagcca atattatttt
     2761 cgtcgacttc cagcaacaag gtgaaagagg actgacgaac gcgcctgatg aagacccgga
     2821 cgatttaagc acgggatatt acggttcagc gtaccggtca ccggagaact ggacgacggc
     2881 actgcgaagc agtcatttca gcacggcagc ccgtcggggg attatttctg acagatttgt
     2941 agaagcaatt ttgcagtttt ggcgcgaaag gtgagcgggg agtttattca tgggggatgt
     3001 ccttgttcgt tctcagagta gacaacaaaa aacaaatgag atcaaggtaa tatctcttca
     3061 cttgcgggct cgaacagcag gcaattgcca atataataat ctggcacaag ggtgaaaaac
     3121 gcgcaccgca taaaccgttg ttattgctta cagattagcc ggatgcctta atagacatcc
     3181 gcgccttttc gattatggct cggaaatcta cgaacatcta cacagtttac tggaacgttt
     3241 tgggccacag cgttcgcagc accgtcctga tatgccgttc tggcgattat aagtcgacgg
     3301 attctggcaa atagacgatg atgatatgct taatgatgtt caaagaaatt aggccattat
     3361 tatattattg cttgtatttt ctttcattcc taaaagcacc cgccattaat cccaggcgca
     3421 ttat
//
LOCUS       DAZSNM010000290         3413 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZSNM010000290 DAZSNM010000000
VERSION     DAZSNM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.290"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..237
                     /locus_tag="ACHW42_003983"
     CDS             <1..237
                     /locus_tag="ACHW42_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFR8945577.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            247..885
                     /locus_tag="ACHW42_003984"
     CDS             247..885
                     /locus_tag="ACHW42_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR8945578.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            888..2051
                     /locus_tag="ACHW42_003985"
     CDS             888..2051
                     /locus_tag="ACHW42_003985"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR8945579.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2135..>3413
                     /locus_tag="ACHW42_003986"
     CDS             2135..>3413
                     /locus_tag="ACHW42_003986"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR8945580.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          759 a          803 c          987 g          864 t
ORIGIN      
        1 ttgttaggtt acgttatccc attatccagt gcggcaatca atgcggtgag tattccagac
       61 tatttcgcct ggggcgggat tgcactggtg attcagttac tcgtttttgc tggtgtcaga
      121 ctttatatgc ccgcattaag cgaaaaaatt attaatcaca ataccgcagc aggaatgttt
      181 atgggaaccg ccgcgctggc tggcggtatt tttaacgcag cttgtatgac atggtaatgg
      241 acgatcatgg ccagaaaacg caaatcaaga aataacagta aaattggtca cggagcgatc
      301 agtcgcattg gtagaccgaa taatcctttt gaaccgcgtc gcaatcgcta cgcacaaaaa
      361 tacttaacgt tggcgctaat gggcggtgcc gcttttttcg tattgaaagg ttgtagcgat
      421 agtagcgatg tcgataacga cggcgacgga acattttacg cgacagtgca ggattgtatt
      481 gatgacggta ataatgccga catttgcgcg cgtggctgga acaacgccaa agcggcattt
      541 tatgccgatg ttccaaagaa tatgactcag cagaactgtc agtctaagta cgaaaattgc
      601 tactacgaca atgttgaaca gagttggatc ccggtcgttt ctggattttt gttaagccgg
      661 gttattcgta aagatcgcga tgagccgttc gtctataaca gcggcggatc ctcatttgct
      721 tcccgccccg tctggcgcaa cacttccggt gattactcct ggcgcttcgg ttctggcaaa
      781 aaagagtctt actcttcagg cggctttacc accagaaaag cgtcgaccgt ttcgcgcggc
      841 ggctatggtc gttcttccag cgcccgtggg cattggggag gctaatcatg ctgagacaca
      901 acgttcctgt gcggcgggat ctggaccaga tcgccgctaa caacggtttc gactttcata
      961 tcatcgacaa tgaaatctat tgggacgaga gtcgggctta ccgttttact ctgcgccaga
     1021 ttgaagagca gatcgaaaaa ccgactgcgg aactgcatca gatgtgcctt gaggtggtgg
     1081 atcgcgcggt taaagacgaa gagatcctga cgcaactggc gatcccgccg ttgtactggg
     1141 atgtgatcgc cgaaagctgg cgcgcacgcg atccttcgct gtatggccgg atggattttg
     1201 cctggtgtgg caatgcgccg gtgaagctgc tggagtacaa cgccgatacg ccgacctcgt
     1261 tgtatgagtc ggcttatttc cagtggttgt ggctggagga tgcccggcgc agcggcatta
     1321 ttccgcgtga tgccgatcag tacaacgcca ttcaggaacg cctgatttcg cgctttagtg
     1381 agctttacag tcgggaactg ttttattttt gctgctgtca ggacaccgat gaagacagga
     1441 gtaccgtgct gtacttgcag gactgcgccc agcaggcagg gcaggagtcg cggtttatct
     1501 acattgaaga tctcggtttg ggcgtcggcg gcgtactgac cgatcttgat gataatgtca
     1561 tccagcgtgc atttaagctg tatccgctgg agtggatgat gcgtgacgat aacggtccgc
     1621 tgctgcgcaa gcgtcgcgag caatgggtgg agccgttatg gaaaagtatc ttgagtaata
     1681 aagggctaat gccgctgctt tggcgcttct tccctggtca tcctaacctt cttgcatcct
     1741 ggtttgaggg tgaaaaatcg cagattgccg ctggcgaaag ctatgtgcgt aaaccgatct
     1801 actcgcgcga aggcggaaac gtcaccattt tcgacggtca gaataacgtc gttgaccacg
     1861 ctgatggtga ttacgccgat gaaccgatga tctaccaggc gtttcaacct ctgccgcgat
     1921 ttggcgatag ctacacactc atcggtagct ggattgtcga tgatgaagcg tgcggaatgg
     1981 ggatccgtga agataacaca ctgatcacca aagacacctc acgtttcgtt ccgcattaca
     2041 ttgctggata agaatgtttt agcaatctct ttctgtcatg aatccatggc agtgaccata
     2101 ctaatggtga ctgccattga tggagggaga cacagtgcac tggcaaactc acaccatttt
     2161 taatcaacct ataccattaa ataacagcaa cttatacctg tctgatggcg cgctttgcga
     2221 agcggtaacg cgtgaaggtg ctggctggga tagcgatttt ctagccagta ttggtcagca
     2281 gttaggaacg gctgaatccc ttgaactggg gcggctggcg aatgtaaacc cgcctgaact
     2341 gttgcgctac gatgcccagg ggcggcgtct ggacgatgtt cgtttccatc ccgcatggca
     2401 cctgctgatg caggcgttat gtaccaatcg ggtgcacaat cttgcctggg aagaagacgc
     2461 tcgctccggc gcatttgtgg cgcgcgcggc gcgttttatg ttacatgcgc aggttgaggc
     2521 tgggtcgtta tgtccagtaa cgatgacctt tgccgccacg ccattgttgt tacagatgtt
     2581 acccacgccg tttcaggact ggaccacgcc gctgctgagc gatcgctacg attctcactt
     2641 attgccaggt gggcaaaaac gcggtttgtt gattggcatg ggaatgacgg aaaagcaggg
     2701 cggttccgat gtcatgagca acaccacccg tgcagagcgt ttggaagatg gctcttatcg
     2761 gctggtgggg cataaatggt ttttctcggt gccgcaaagc gatgcgcatc tggtgctggc
     2821 gcagaccgcg ggcggtctgt cctgcttttt tgtgccgcgc tttttgcctg acgggcaacg
     2881 caacgcgatt cgcctcgagc ggctgaaaga taagctgggt aatcgctcta acgccagttg
     2941 cgaagtggag tttcaggatg ccattggctg gttattgggg caggaagggg aaggaattcg
     3001 tctgatcctg aaaatggggg ggatgacgcg ttttgattgc gccctgggta gccatgccat
     3061 gatgcgccgt gcattttcgc tggcgattta tcatgcacat caacgccatg tttttggtaa
     3121 tccattgatc caacagcccc ttatgcgtca tgtcttaagt cgcatggcac ttcagcttga
     3181 agggcaaacg gcgttgctgt ttcgtcttgc gcgagcgtgg gaccggcgtg ccgatgccaa
     3241 agaagccctg tgggcgcgtt tatttacgcc tgcggcaaaa tttgtgatct gcaaacgagg
     3301 tatgccgttt gtggccgaag cgatggaggt gctgggcggc attggttatt gcgaggagag
     3361 cgagctgccg cggctttacc gggagatgcc ggtaaacagt atttgggaag gtt
//
LOCUS       DAZSNM010000291         3412 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZSNM010000291 DAZSNM010000000
VERSION     DAZSNM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.291"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            259..594
                     /locus_tag="ACHW42_003987"
     CDS             259..594
                     /locus_tag="ACHW42_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR8945581.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            752..1195
                     /gene="yjdN"
                     /locus_tag="ACHW42_003988"
     CDS             752..1195
                     /gene="yjdN"
                     /locus_tag="ACHW42_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFR8945582.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1328..2116
                     /gene="phnC"
                     /locus_tag="ACHW42_003989"
     CDS             1328..2116
                     /gene="phnC"
                     /locus_tag="ACHW42_003989"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR8945583.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            2141..3157
                     /gene="phnD"
                     /locus_tag="ACHW42_003990"
     CDS             2141..3157
                     /gene="phnD"
                     /locus_tag="ACHW42_003990"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8945584.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3263..>3412
                     /locus_tag="ACHW42_003991"
     CDS             3263..>3412
                     /locus_tag="ACHW42_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HFR8945585.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATF"
BASE COUNT          884 a          940 c          864 g          724 t
ORIGIN      
        1 atgcgtttaa aacgtcagaa agataaaaat atcatgtgaa taaaaaaaag aacaagtaga
       61 gcattaacat tatcttaaat aataaataga ggcaaaaaga ttattttctt tttgcgtttc
      121 ctttcaaatg aaaacgatcg tcgtctaaaa tcagcagtac ccccgacaaa ctcagggatt
      181 ttgtgtataa ttgcggcctt tttcggcaat ctgccgtttt ttggcgcttt tgccctgctg
      241 acttttgagg aaatccacat gtcattacca cactgcccaa aatgcaactc cgaatacact
      301 tacgaagata acggcatgta catctgcccg gaatgtgcct acgaatggaa cgacgcagaa
      361 cctgcacagg aaagcgacga gctgatcgtt aaagatgcta acggcaatct gctggctgac
      421 ggcgacagcg ttaccatcat taaagatctg aaggtgaaag gtagctcttc gatgctgaaa
      481 attggcacca aagtgaaaaa catccgcctg gttgaaggcg accataacat cgattgcaaa
      541 atcgacggtt ttggtccgat gaaactgaaa tctgagtttg tgaaaaagaa ctgattgtat
      601 tgtgatcggt aagccggata aggcgctcgc gccgcatccg gcaacgatgc cgactgcctg
      661 atgcgacgct tgcgcgtctt atcaggcagc ctctcatctg tataaatttc gaactacact
      721 taactggctt ctcttaactg aggtcaccat catgccgtta agtccctacc tctcttttgc
      781 cggtaactgt tccgacgcga ttgcctatta tcaacgtacg ttgggcgcgg aactgctcta
      841 taaaatcagc ttcggcgaaa tgccgaaatc agcgcaggac agcgccgaga actgcccttc
      901 cggaatgcaa tttcccgata ccgccatcgc tcatgccaac gtgcgcattg ccggaagcga
      961 catcatgatg agcgatgcta taccgtcagg aaaagccagc tactccggct ttacgctggt
     1021 gctcgattcg caacaggtcg aagaaggaaa acgctggttt gataaccttg ccgctaacgg
     1081 aaaaatcgaa atggcctggc aggaaacttt ctgggcgcat ggctttggca aagtcaccga
     1141 taaatttggc gtaccgtgga tgattaatgt cgtcaaacaa caaccaacgc aataacccgc
     1201 cgggaggccc gccctcccgc actgtcatcg aattcccgtt aactcttcat ctgttagtca
     1261 cttttaatta accaaatcgt cacaataatc cgccacgatg gagccacttt tttagggagg
     1321 ctgcatcatg caaacgatta tccgtgtcga gaagctcgcc aaaaccttca atcagcatca
     1381 agcgctgcat gcggttgatc tgaacattca tcacagtgaa atggtggctc tgcttgggcc
     1441 gtcgggttcc ggcaaatcca cccttttacg tcatttaagc ggtttgatta ccggcgataa
     1501 atccgccggt agccatatcg agctactggg ccgcactgtc cagcgcgaag gccgcctggc
     1561 ccgcgatatc cgcaaaagcc gcgccaacac cggctacatc tttcaacaat tcaacctggt
     1621 gaaccgcctg agcgtactgg agaacgtgct gattggcgcg ctcggcagca cgccgttctg
     1681 gcgcacctgt tttagctggt tcaccggcga gcagaaacag cgcgcgttac aggcgctgac
     1741 ccgcgttggc atggtgcatt ttgcccatca gcgcgtttcc accctctccg gcggccagca
     1801 gcaacgtgtg gcgattgccc gtgcgctgat gcagcaggcg aaagtgattc tggccgatga
     1861 acccatcgcc tcgctggacc cggaatcagc gcgcatcgtg atggacaccc tgcgcgacat
     1921 caaccagaac gacggcatca ccgtggtcgt cacgctgcat caggtggatt acgccctgcg
     1981 ctactgcgaa cgcatcgtcg ccctgcgtca ggggcacgtc ttctacgacg gcagcagcca
     2041 actgtttgat aacgaacgtt ttgaccatct ctaccgcagc attaaccgca tcgaagagaa
     2101 cgcgaaagct gcctgatatc cccatcattg aggaaaacga atgaacgcta agataattgc
     2161 ctcgctggcc ttcaccagca tgttcagcct cagcaccctg ttaaccccgg cacacgccga
     2221 agagcaggaa aaggcgctga atttcggcat tatttcaacg gaatcacagc aaaacctgaa
     2281 accgcaatgg acgccgtttt tacaggatat ggagaagaaa ctgggcgtga aggtcaacgc
     2341 cttctttgcc ccggactacg cgggcattat ccaggggatg cgcttcaata aagtggatat
     2401 tgcctggtac ggcaacctgt cggcgatgga agcggtggat cgcgccaatg gccaggtctt
     2461 cgcccagacg gtcgcggcgg atggatcgcc gggttactgg agcgtgttga tcgtcaacaa
     2521 agacagcccg atcaacaacc tgaacgatct gctggcgaag cggaaagatc tcaccttcgg
     2581 caatggcgat cctaactcca cctctggctt cctcgtcccc ggttactacg tcttcgctaa
     2641 aaacaatatc tccgccagcg acttcaagcg caccgtcaac gccgggcatg aaaccaacgc
     2701 gctggccgtc gccaacaagc aagtggatgt tgccaccaac aacaccgaaa acctcgacaa
     2761 gctgaaaacc tccgcgccgg agaagcttaa agagctgaag gtgatctgga aatcgccgct
     2821 gatcctaggc gatccgatcg tctggcgcaa gaatctgtcc gaaactacca aagacaagat
     2881 ctacgacttc tttatgaact acggaaaaac gccggaagag aaagcggtgc tggaacgcct
     2941 gggctgggcg ccgttccgcg cttccagcga cctgcaactg gtgccgattc gccagctcgc
     3001 actgtttaaa gagatacaaa gcgtgaaagg caataaaggg ctgaatgagc aggacaagct
     3061 ggcgaaaacc accgagattc aggcgcagct ggatgacctg gaccgcctga acaacgcgtt
     3121 aagcgcgatg agttcggtga gtaaagcggt gcagtaaatc gtaggtcgga taagacgccc
     3181 cggcgtcgca tccgacaatg tgtaggcgtt gatgccggat gcggtgcaag caccttatcc
     3241 ggcctacaga ccggagccaa acatgcaaac catcaccatc gccccaccca agcgtagctg
     3301 gttctcgctt ctgagctggg ccgttgttct cgacgtgctg gtcgtctcgt ggcagggcgc
     3361 ggaaatggcc ccgcttacgc tgattaaaga cggcggcaac atggcaacct tc
//
LOCUS       DAZSNM010000292         3411 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZSNM010000292 DAZSNM010000000
VERSION     DAZSNM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.292"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..465)
                     /locus_tag="ACHW42_003992"
     CDS             complement(<1..465)
                     /locus_tag="ACHW42_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8945586.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
     gene            complement(452..829)
                     /locus_tag="ACHW42_003993"
     CDS             complement(452..829)
                     /locus_tag="ACHW42_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR8945587.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            complement(841..1227)
                     /gene="gpFI"
                     /locus_tag="ACHW42_003994"
     CDS             complement(841..1227)
                     /gene="gpFI"
                     /locus_tag="ACHW42_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR8945588.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            complement(1279..2307)
                     /locus_tag="ACHW42_003995"
     CDS             complement(1279..2307)
                     /locus_tag="ACHW42_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR8945589.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            complement(2365..2712)
                     /locus_tag="ACHW42_003996"
     CDS             complement(2365..2712)
                     /locus_tag="ACHW42_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR8945590.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            complement(2749..>3411)
                     /locus_tag="ACHW42_003997"
     CDS             complement(2749..>3411)
                     /locus_tag="ACHW42_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR8945591.1"
                     /translation="ASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQ
                     EAKGREQLATMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQL
                     AATLAAMPEMTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMT
                     VENARAVLAAASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMA
                     MP"
BASE COUNT          731 a         1072 c          806 g          802 t
ORIGIN      
        1 cgggattttc accacatcaa tggggtaacg atttttgccg tcaatacgcc gcatcacatg
       61 ccagcgacca ttcgccagtt gctgaataaa cgcatcccgg aaaaggtatt tccccacttt
      121 cagtacgctg ccacgatgac gtgagttgct gccatgccgg gagcgggcca gtctgacctg
      181 tgcggtaccc agcttaatgg cgggcaggtt accccggtta acacgcatgc gggcatacat
      241 ttttcctgag gggctggctt taagcagcct gacacgctcc ctgaccgttt tcaggggaat
      301 acctttcacc tggttatccc cggcaacggt attctgcgca acctgccgtg tggcgacaga
      361 aacaatcttt gccgccacac ggtttaccgc ccatgcgctg gtctgtggca ccatgcgggt
      421 atcgaggctg ttcagattgc ggatggcatt ctcaagtccc ttcatcatat cgcctcattc
      481 tggcattatt ctgtggggca ggactgcccc gccttaaccg gatataacag catcccccgt
      541 catccggagt aatacgatcc acaaagaaag aatcatcccc gatagttaac gtatcctgac
      601 ggcgtaactg cgatatatct gccgttttca caaacaggca cggtgaggaa tcctcaagcc
      661 ggatcccccc ggcaacaaaa gaaatacttt cgggatcatc aaaaacccct gtcaggtgtg
      721 tcccctcaag ttctccggac gtaatgacgg cgctgatccc catatcacgg agaatgactt
      781 cattgacacc ggcaagcgca gcatcaaaca gattatcgaa accgtccacg acatctcctg
      841 ttactgcctt tttgccagac cacctgccgc catgctggca gccacctcag cagaaacacg
      901 aaacgacgtt cccggtgaaa caaatatcac tgaaccatta cattttgtat gcagtgtcgc
      961 cagtgccacc accgtaacct gccccgtttc gtctccggta acaatgcctg gctgttcagg
     1021 aactgactcg cctttatgcg cggtcggatc atctttcgat ggtgagtacg tatccggctc
     1081 tgcgacctcc cccgcatctc caagctcttc ctccagttcc gccactcgct gcgcaatatc
     1141 tgccgcacta ccggagacat ccggttcacg ccccagcctc tcagccagct cacaaagacg
     1201 tttcaggttt tcttctttcg tagccatacc cgctcctgat tgaatgaaaa acgggagcat
     1261 ttagctcccg ctcacggatt atttcacctg caccaccaca aactcatccg gatccggcag
     1321 caccatcagc ggcgcggact gcgtcatggt gaattcacgc gccggatcac ccacggtcag
     1381 ccagtgtttc ggataacggg aagacgccac cacgccttca gacaatgcct gcgcatcctg
     1441 aatggcaccg tagcaacgaa taccctctgc ggccgtattt cccagtacca gtgtgccgtc
     1501 cggcagataa cgtttttcgg taccgtcctc tgccacatag gacgtttttg ccaccacgat
     1561 tgccagatca ccataatacc ctttgaagga caccaccgcc cccaggtctt tcactgccgt
     1621 ttccagctgt gaatttgagc cacgacgggt atccagtttt tcgcggaaca gcttaaagcc
     1681 attcagcaga cgccagacct taccatccat aatggcaata ttgatggtgc cggaagcaaa
     1741 atcacagtac gcatccagat catgcgtcgg atcaaaggta tcgcggtcct gagcgctcca
     1801 ctccgtccct cctgcctgcg taatgttatt tcccaccgac cggccaaaat cgacctcaac
     1861 agtttcaaac tgctctcctt ccatggtgta tttgccatac agcacggcat tcaccgcctg
     1921 catctcttcc acctggacaa tggcgtgctc ttcctgtttg aggttatcgg tgatgatacg
     1981 caggcgacgg taagccgggc cgttcagctg ggccggatct tcaccgggaa gacgctccac
     2041 cgcctgctgg taattaaatt cgtgtttcgg cttaacgtag cccgggcgca gtacacgagt
     2101 ttcaccgcca cggtgacgca acacctttcc ttcaacaacc ggggagacat aggccgccac
     2161 cggcgttttt ccggtaattt tgtccagcat tacctcttcg gtatggaaat tcaccgtacg
     2221 gcggaaaaac agctccagaa acagcgcacg aaatttaact ttttgttcgg tataaccgag
     2281 taactggcgg gtcgtaaaca atcccataaa tcagttcctt tcattcagaa atcagtcagg
     2341 ctaccgcagt gacctgataa aatgttacgg cagcgctgcg tgactcaggg cactgccggc
     2401 aaaggcattc gcctttttgt gtgtatccac tctctcaggc cagtggatgg cctccgtcgc
     2461 aaaggtcccc gacttgtaat acgtcagcac cgtctctgta ccttcaagcg gcagtaccag
     2521 tatgccaacc acatggcctg ccttctgtcc gtcccagacc accagtttcc cggtatcctc
     2581 atccagcatc aggggcgtca gagccggtgt tgccgaggaa atcccgctgc agcctgtggc
     2641 ggtatgggcc ggatcattac cggcaaaaat acgtacttcc gcacgctgtt cagtgatggt
     2701 tttcgttacc atattgtaaa aacctcatat tgatggtcag cactgacttc atggcatggc
     2761 catgagcatt ttcacgtccg catcaccgtc tgctgacgtc tgtggcacgc caccctgtac
     2821 cgctgccggt gaatggttcg ccatgaaatg ttcaaacatg gcggttgtgg atgcagaaac
     2881 cggttctgcc ctgcctgagg ctgccgccag tacagcccgg gcattctcca cggtcatccc
     2941 cggacaggcc gccagttttt cagcctgcgc ctctgcccct tttgcctcat ccagggccat
     3001 aatctgatca cgaagtgagg gtccggcatt cgcctgcggt gaagcagcca gaatcggacg
     3061 ggctttttcc accgtcatct ccggcatcgc cgccagcgtt gccgccagtt gttcacgtcc
     3121 tttcgcctct tcacatgcca taatgcgatc ggcttcactc tgcgcagata caccagattg
     3181 ttgcggtgcc gccacggcca gaatcgcccg ggcctgctca acgctcatac cctgttgccc
     3241 tgccagcatc gtggccagtt gttcacgtcc tttcgcttcc tgacacgtca ggatccccat
     3301 cactcgctgg ttctcctgcg cggcagcctc cgttgcagtt aattgcggca tagtgcctcc
     3361 tgtatcgtgt gtgttcagcg tcgcagccat cacgctgatg gcgtccgacg c
//
LOCUS       DAZSNM010000293         3405 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZSNM010000293 DAZSNM010000000
VERSION     DAZSNM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.293"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(226..429)
                     /locus_tag="ACHW42_003998"
     CDS             complement(226..429)
                     /locus_tag="ACHW42_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945592.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="ACHW42_003999"
     CDS             complement(431..586)
                     /locus_tag="ACHW42_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR8945593.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="ACHW42_004000"
     CDS             complement(789..1196)
                     /locus_tag="ACHW42_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8945594.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="ACHW42_004001"
     CDS             1273..1500
                     /locus_tag="ACHW42_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR8945595.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="ACHW42_004002"
     CDS             1484..1906
                     /locus_tag="ACHW42_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR8945596.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="ACHW42_004003"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="ACHW42_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3405
                     /locus_tag="ACHW42_004004"
                     /pseudo
     CDS             2768..3405
                     /locus_tag="ACHW42_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          878 a          753 c          916 g          858 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acatt
//
LOCUS       DAZSNM010000294         3376 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZSNM010000294 DAZSNM010000000
VERSION     DAZSNM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.294"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            51..260
                     /locus_tag="ACHW42_004005"
                     /pseudo
     CDS             51..260
                     /locus_tag="ACHW42_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234527.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            321..509
                     /locus_tag="ACHW42_004006"
     CDS             321..509
                     /locus_tag="ACHW42_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945597.1"
                     /translation="MNIYFRIVISLAIIACIYGLIVPALISMKDTVAVISGFALACLT
                     PPCIYAIYKGLSFSKDKR"
     gene            506..1333
                     /locus_tag="ACHW42_004007"
     CDS             506..1333
                     /locus_tag="ACHW42_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR8945598.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1377..2126
                     /locus_tag="ACHW42_004008"
     CDS             1377..2126
                     /locus_tag="ACHW42_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR8945599.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2123..2692
                     /locus_tag="ACHW42_004009"
     CDS             2123..2692
                     /locus_tag="ACHW42_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945600.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIWTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2689..2811
                     /locus_tag="ACHW42_004010"
     CDS             2689..2811
                     /locus_tag="ACHW42_004010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945601.1"
                     /translation="MKIEYQDAAGGESMSWPDAIVTLGMVFAVAFVVYSICRWG"
     gene            2816..3058
                     /locus_tag="ACHW42_004011"
     CDS             2816..3058
                     /locus_tag="ACHW42_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR8945602.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3062..3208
                     /locus_tag="ACHW42_004012"
     CDS             3062..3208
                     /locus_tag="ACHW42_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945603.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3217..>3376
                     /gene="xisR"
                     /locus_tag="ACHW42_004013"
     CDS             3217..>3376
                     /gene="xisR"
                     /locus_tag="ACHW42_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFR8945604.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPK"
BASE COUNT          946 a          808 c          863 g          759 t
ORIGIN      
        1 ctgcttaaaa aagaacaaat ggtagtgtcc gctattgcca gtacacctca caacgccatc
       61 ctgacaagcg ttttatcttc aacgggaatg agatatggaa acgtggagaa tcaaccacag
      121 agtatacccg cgcacaccag cgaacaatca gacgactgcg cagactcatc gccaggaaac
      181 agtatacaga aaaatggttc agaaaatatg acacatggag cgccggatat tacgaactga
      241 tggcaacaaa accattctga cgtaaacgaa attaaccatg acgcaattaa ataaggcaag
      301 ccgaatacat caggaggacc atgaacattt atttcagaat agttatatca ctggcaatta
      361 tcgcatgtat ttacggatta atcgttccgg ccctcatatc aatgaaggat acggtagcag
      421 tgatttctgg ctttgctctg gcgtgtctga ccccgccctg catttatgcc atttataagg
      481 gtctttcttt ttctaaggat aaaagatgaa aaaagttatt cctgctttag cccttgttct
      541 gctgaccacc ggacttgtcg gttgcgatcg cgttgagcct ggtaatgttg gcatcaaggt
      601 aaacaaactg ggcgacgata aaggcgtcgg agaagtggtc ggtgttgggc gctactggac
      661 gggatggaac actgaggttt acatcttccc gaccttcaaa caaatgaaga cctacgatga
      721 gccattcagc ttccagatga gtgacggtac aaccatcggc tatcacatcg gcgtggccta
      781 caaagttgat ccatccaaag ttaccacagt ctttcagacc taccgcaaag gcgtggacga
      841 cattaccgac accgacctgc gccagaagat agccgacgca ctcaaccgac tggccagcaa
      901 aatgaccacc gacaaattta tcgacggcgg caagtctgag ctgctggatg cagctcttaa
      961 agacattcag gaagaaatga cgcccatcgg tattcaggta atgagcctct catatgtggg
     1021 taagccggag tacccgccta ctgttatcga cagcattaat gccaaagtca cggcgaacca
     1081 gaaaaccctg caatgcgagc aggaagtaaa acagcgcgaa gcggaagcca acatgttgcg
     1141 cgcggaagct gccggacagg cagatgcgat tcgcacaaaa gcccaggccg aagccgatgc
     1201 tattcgttta cgcggtgaag ctctgcgcca gaacccaaac gttatggagc tggaagccat
     1261 caacaagtgg aacggtacac tgccgcaata catgaccagc ggtgccaaca caccatttat
     1321 tcaggttaag taatacatat gcccggtatt acacgccggg ctgtctggag ataaaaatga
     1381 atactgtaac catcaacaac aaacagtttc cggtaatcga atatcgcggt cagcgtgttg
     1441 tgacgctggc gatgattgat gaagtccacc agcgaccgga aggaaccgcg cgtgcagcat
     1501 tcaaccgaaa ccgtgagcat ttcatcagcg gtgtggatta tgccgaatta ggtgcggacg
     1561 taatacgtac ggacctcccg gaaggaacat tctctaaatt tgcaccatcg gggattgtac
     1621 tttttgaatc aggttacctg atgttgacga agccattcaa tgacgccctt gcctggcagg
     1681 ttcagcgcga actggttaac agctacttcc gcactcgcac accgctaacg gaaatcgaga
     1741 tgatcgctgc gatagccgcc gacgctgttc gtcaacagaa gcgcctgaat catgtggaag
     1801 aacagctcga aacagtcaca gaagcggtgg aaaccatcaa acgcggaaat atgcgcgccg
     1861 gatatgtcgg ttaccgccag gtggtagcca aaagtggaat gagtgacacc aagtgtcgga
     1921 atctggtgaa cgcatatcaa attcctacag acacccacga attcatgact ccggacggtc
     1981 tgttgtctcg cagggctatc gtcgagcttg agccatttat ggcggcgttc caccaaatga
     2041 tgtcagaggc tgacccacgc ggcacacgct ggtatcaccc gaagatgggg cttttccagg
     2101 tgatcggatg ggaggataaa gcatgatcat ccagtcaaaa cttattcgcg ccgctctggt
     2161 gtgcgctgct aaaaacgacg ttcgttatta cctgaacggt cttcacatca cgccgaaaca
     2221 tattgaggca accaatggtt ccgtggcact gcgcatggct cacggcatct ggacgaagaa
     2281 aaacatcatt gtccagttcg aagggggcgt cccggccaaa gccgaaacga cagagctgat
     2341 ttttagtaaa gagccgatcg ctgttcatcg cgaccagttt cagcgacgac tgtccattac
     2401 cggcattaaa ctggtggacg gttgttttcc ggatttggat cgcatcattc cgaaaaagtt
     2461 tgaccgctgt actcacccgg tgctccaggc gggttacctg agttatccag agaagatgtt
     2521 tggtcgtgag cgtaaattta ttcccgtcca gttacgtccc tccggtgacg ggcaagcggt
     2581 cagaattcag tttgattcca tcatcaactc aatgtatggc aatcctgaat ttgttgtgat
     2641 gccttgtcgt gatcatggcg atttcaacgt ggctcaggag catccggaat gaaaatcgaa
     2701 taccaggacg ccgcaggagg tgaatcaatg agctggcctg atgcaatcgt aactctgggg
     2761 atggtattcg cagtagcgtt tgttgtgtac tcgatttgtc gatggggata accacatgtt
     2821 cgctttgatt caacgcgggc agatatacac agacagagcc ggatacccag tagaaattac
     2881 tcgcagtact gagcactcag tgttctttcg gaggatggac ggacgtaccg ggcgagtacg
     2941 catcggtgaa ttcagtagcc tgttcgagca tattgaccac ctggaatatc acaagattct
     3001 ggctgaaaca gaacaagaaa agcacctgaa gaaattacgc gcaatgcaaa ggaagtaaaa
     3061 aatgaataaa gcgtttgagc tatgggtcca ccagcgttac ggcaaccact acgacctgac
     3121 gcgagatgtt gacggtttct actgtcgtga aattgtgaaa cgaatgtttg aggtctggtg
     3181 ccactgccgt gggctgagtg ttgtgtgagg taatgcatgg gcaatgtgat tcaactggct
     3241 cccaatgaat gggtttgtga aagcgttcta atcgcaatta ccgggctcaa accaggcaca
     3301 attcttcgga cccggaaaga atgctggatg gttggaagag agtatattca cgtatcacca
     3361 gacggtaatc caaagc
//
LOCUS       DAZSNM010000295         3347 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZSNM010000295 DAZSNM010000000
VERSION     DAZSNM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.295"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            18..896
                     /locus_tag="ACHW42_004014"
     CDS             18..896
                     /locus_tag="ACHW42_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR8945605.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            893..2284
                     /locus_tag="ACHW42_004015"
     CDS             893..2284
                     /locus_tag="ACHW42_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR8945606.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2281..2538
                     /locus_tag="ACHW42_004016"
     CDS             2281..2538
                     /locus_tag="ACHW42_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945607.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2633..2833
                     /locus_tag="ACHW42_004017"
     CDS             2633..2833
                     /locus_tag="ACHW42_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFR8945608.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2826..3206
                     /locus_tag="ACHW42_004018"
     CDS             2826..3206
                     /locus_tag="ACHW42_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR8945609.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          885 a          725 c          969 g          768 t
ORIGIN      
        1 attctgaggt gacagcgatg atgacgttta acctgcgtga acaacaaaaa agactacagg
       61 cgcgaatgga tgagttacgg gcagagattg catttgctca gaagggcgaa aagccatggc
      121 cttatcgttc ctgcctgatg cgtgaaggtc gcggatattg cgaaaaacat ggcgaatatc
      181 acacgcatat actggtgtgg agcgatcgta atggcgagga cagagaagaa atttcatgct
      241 gccctgactg cttaatcgct gaggccaacg atttgaccat ggagctgtcg tccatcaagg
      301 cggaagagct gactgataac gccggaattg ccctgcgttt tcgggactgc gagtttgata
      361 attatctgga ggttaatcct ggcgcagcca gaaatcttgc ggcctgtcgc cgctatgcgg
      421 agaactggcc agatatgctg gagaacggta ccagtcttgt tatgaccggc agttgtggta
      481 ccggaaaaaa tcatctggct gtggctatgg caaaacacat catccgtaac tatctggcca
      541 gtgtggagat caccgacgtg atgcgcctta cccgtgctgt gaaaaattgc tggcggaatg
      601 acagcgaaaa aacagcggat gaagttattg agcgttatgc gtcaatggat ttgctgatca
      661 tcgacgaagt tggcgttcag tttggcagcg cggctgaaat ggctattttg caggaaatta
      721 tcaacgccag gtacgaaagt atcttgccca ccattctgat cagtaacctc tcaccggaag
      781 agttgtgggc gttcatcagt ccccgaattg ccgacaggat cacagacggg ggacgcaact
      841 ggttgtcgtt taactggccc agttaccgtt ctcgtatcag aggtgtggct gcatgacaac
      901 tccagtatgg cgtaacgatg accttgaagg cgctgtcatc ggcgcgttct ttctgcgcgg
      961 agcagatcct gaagtgatgg atattctggc cacactgcca gcggacgttt tttctgtacg
     1021 agcgtatcag gatatctata caggcatctg cagacaggcc cgtgtttcag gagtgattga
     1081 ccccgtgctg ttgtgtaatg agatgccgga acttgccccg gtgattactg ataccgggcg
     1141 taaaacctgg gtgaagtctt cactggagca ctatgttgca gcgttgcggc gcaatgccgc
     1201 actgcgcgat gcagaaaaaa cactgaatga ggcgctgcag aaattacgtg atgcgcatac
     1261 ctgtgaagca gctgaagatg ccctgaagga tgcgcagaac atgatggtca cactgtcgac
     1321 aggaaagggc gtcattcagc cggtacatat tgatgatgtg cttccggaag tggttgagcg
     1381 tgttgaatgc cggaatcagg ggctggagaa atccaggacg ttgatgaccg gtattgatga
     1441 actggacgca aaaacaggcg gtatggagcc cggagacctg gtattcattg ccgcccgtcc
     1501 ttcgatgggg aaaaccgaac ttgcgctgga catcatcgac aaggtgactg agcaggggca
     1561 tggtgtgctt ctgttcacca tggagatggc gaacatccag attggtgaac gtatggtgtc
     1621 tgcggctgga gggatgccag tatcacgcct gaaatctgtg gctcactttg aagatgaaga
     1681 ctggacgcgt ttctcacaag gggtggggcg gatgaccggg cgcaatatct ggatggtgga
     1741 ccaggcgaac ctggccattg atgagatatg cgcaacaacg aaacaccacc tgattaaata
     1801 tccggaaacg gcgctggtgg tggttgatta tctcgggctg ataaaaaccc gaaccacggg
     1861 gcgtcatgac cttgccgtgg gtgaaatctc aaaggggctt aaaggcctgg caaaatccgg
     1921 tggttttccg ttgattgcgc tgagccagct ctcccgcggt gtggagtcca gacccaataa
     1981 acgtcccatg aactcagacc tgaaaaattc cggagaaata gaggcggatg cagacatcat
     2041 tctgatgctt tacagggatg aagtgtacaa cccggatacg caggccaggg gcatcgcaga
     2101 aatcaatatc acgaaacaac gtaacggttc tctgggaacg atttaccggc gtttttataa
     2161 cggacatttt ctgcctgtgg accaggaaag tgcacgggtg ctttccactc ccatgaagcc
     2221 cggcaatccg cgcagataca gtaataaacg aactgacagc agtaagatgg agcgtttctt
     2281 ttgaacaacc agacaatgac ttttacccct gaacaattgc gtaaacacgc gcaggaaatg
     2341 ctgcgtcatg ctgaacaact cgaaaaaacg ggcataacaa aagatgctat ccgtaaagat
     2401 atggtacccg cgcttcggga actgatgcag gcgaagcatc gcgcacagaa agcagtagat
     2461 gagctggtgg actgtgtggc agagctggaa accagagtcg gaaagtttga aaaactggtg
     2521 caggaggcgc tgcgctgatg cgccatgagt ttgttttgcc ctggcttgtg cgcgatcatt
     2581 atgtccctgg tggaaagccc gggataaaaa tcaccgaacc ggagagtatt tgatgagcgt
     2641 caaaattcaa acgataccgg aactgcttat ccagacacga ggtaatatga ccgaagtttc
     2701 acgaatgctg aactgtaatc gcgccacggt aagaaaatat gctgaagata aagagggcaa
     2761 agggcacgcc attgttgatg gtgttctgat tgttcatcgt ggatgggaca ggggtaaaga
     2821 cagtgatgcg tgatattcag caggttcttg agcgctgggg cgcgtgggtg gctaataatc
     2881 atgaggatgt gacctggtcg tccattgctg ccggttttaa gggattaatc ccttcaaaag
     2941 taaaatcccg cccgcaatgc tgtgacgatg acgcgatggt tatatgtggg tgtatggccc
     3001 gccttaaccg gaacaacaac gatctacatg acttgctggt tgactactac gtgtttggga
     3061 tgacattcat gacgctggcg cggaagcatg gatgctcaga tggttatata ggaaagaagc
     3121 tacaaaaagc cgagggggtg gtggaaggaa tgttgatgat gttgggtgtc cggttagaaa
     3181 tggacaaata tattgaacat ccctgatggt aaggggcgac tttctagaaa atattttacg
     3241 tacgtaaaaa acgggatatt ctgttaagag tggttgcttc gccacacagc ttaaacccgc
     3301 ctccgggcgg gttttttatg cctgaaaaac ggtacaggac gttaaac
//
LOCUS       DAZSNM010000296         3333 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZSNM010000296 DAZSNM010000000
VERSION     DAZSNM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.296"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            339..1397
                     /gene="rfaQ"
                     /locus_tag="ACHW42_004019"
     CDS             339..1397
                     /gene="rfaQ"
                     /locus_tag="ACHW42_004019"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR8945610.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1394..2518
                     /locus_tag="ACHW42_004020"
     CDS             1394..2518
                     /locus_tag="ACHW42_004020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR8945611.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2511..3308
                     /gene="rfaP"
                     /locus_tag="ACHW42_004021"
     CDS             2511..3308
                     /gene="rfaP"
                     /locus_tag="ACHW42_004021"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR8945612.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT          871 a          730 c          820 g          912 t
ORIGIN      
        1 tttgacttaa atagcaattt acccgccaat tcttgttgaa catctatact ttttttgtat
       61 ttttatgcga ttcattgatt tctgctgtac gtgcaggaag gaatcgcaga gcaacagtga
      121 aagaatatgg ttgctaatga ttttatttga ttgatttctt tattgaaaat caacaatgtg
      181 cttaaaaatc attcgggtcg gattgccgcg aaagaagtga tacactagcg cgtcaatatc
      241 agtgcggtat cagtattcag gtagctgttg agcctggggc ggtagcgtgc tttttctgct
      301 tcaccttaat cggataatct caacaaaaag agttacttgt ggataagcca tttcgaagaa
      361 ttttgctcat taagatgcgt tttcatgggg atatgttatt aactactccc gtcattagtt
      421 ctctgaaaaa aaattaccct gacgcaaaaa tcgatgtgct gctttatcag gacaccatcc
      481 cgatcctgtc tgaaaatcca gagattaacg cgctctacgg cataaaaaat aaaaaagcaa
      541 aagcctcaga aaaaattgcc aacttttttc atctcatcaa ggtattacgt gccaataagt
      601 atgaccttat cgtcaatctt accgatcaat ggatggttgc tatactggtt cgcttattaa
      661 atgctcgtgt gaaaatttcc caggattatc atcatcggca gtctgctttt tggcgtaaaa
      721 gtttcaccca tttggtgccg ttgcagggtg gaaatgtggt ggaaagtaac ttatccgtgc
      781 tgacaccatt gggacttgat tcgttggtga agcagacaac catgagttac ccgcctgcaa
      841 gctggaaacg tatgcgtcgc gaacttgatc acgctggtgt tggacaaaat tatgtggtta
      901 tccaacctac ggcgcggcaa atcttcaaat gctgggacaa cgccaagttt tccgctgtga
      961 ttgatgcctt acatgctcgt ggttatgaag tcgttctgac gtccggccca gataaagacg
     1021 atctggcctg cgtcaatgaa attgcgcagg gatgccagac gccaccagta acggcgctgg
     1081 ctggaaaggt gaccttcccg gaacttggtg cgttaatcga tcatgcgcag ctgtttattg
     1141 gcgttgattc cgcaccggcg catattgccg ctgcagttaa tacgccgctg atatcgctgt
     1201 ttggcgcaac agatcatatt ttctggcgtc cctggtcaaa taacatgatt caattctggg
     1261 cgggagatta ccgggaaatg ccaacgcgcg atcagcgtga ccgaaatgag atgtatcttt
     1321 cggttattcc ggcggcagat gtcattgctg ctgtcgataa attactgccc tcctccacga
     1381 caggtacgtc gttatgatcg ttgctttttg tttatataaa tattttccct ttggcggttt
     1441 gcagcgcgat tttatgcgta ttgctcagac agtcgccgcc cgaggtcatc atgttcgggt
     1501 ttatacccag tcgtgggaag gcgaatgccc tgatgtattt gaagtgatca aagtgccggt
     1561 taaatcgcat accaatcacg ggcgcaatgc ggagtatttt gcctgggtgc aaaaacattt
     1621 acgcgaacat cccgtcgata aagtcgttgg attcaacaaa atgccggggc tggacgttta
     1681 ttatgccgct gatgtttgtt atgccgagaa agtagcgcag gaaaaaggct ttttctatcg
     1741 cctgacgtca cgttatcgcc attatgccgc ctttgagcgg gcaaccttcg aacagggcaa
     1801 gccgacacag ctgctgatgc tgacagataa gcaaatcgcc gatttccaga aacattatca
     1861 gactgaagcg gagcgttttc atattctgcc accggggatt tatcctgatc gtaaatatag
     1921 ccagcagcca gccaatagcc gtgaaatctt ccgtaagaag aatggaataa ccgaacaaca
     1981 atatttattg ttgcaggtcg gttcagactt cacgcgtaaa ggtgccgatc gttccattga
     2041 agcacttgct tcgttaccgg attcgctgcg ccacaacaca ttgctatatg ttgttgggca
     2101 ggataaaccg cgaaaatttg aggcactggc agaaaaacgc ggcgtgcgca gtaatgttca
     2161 cttcttctcg gggcgcaacg atgtctcgga attaatggcg gcggcggatt tattactgca
     2221 tcctgcctac caggaagcgg cgggaattgt gctgctggaa gcgattactg caggattacc
     2281 ggtactaaca actgccgttt gtggctatgc gcattatatt gtcgacgcta attgcggcga
     2341 ggctattgct gagccattcc gccaggaaac attgaatgag attttacgca aagcgttaac
     2401 gcaatcttca ttgcgccagg cttgggcgga aaatgcgcga cattatgctg atacacaaga
     2461 tttatacagt ctgccagaga aagcggcgga tatcataacg ggtggtctgg atggttgaac
     2521 ttaaagagcc gtttgccacg ttatggcgcg gcaaagatcc ttttgaggaa gttaaaacct
     2581 tgcagggtga ggtatttcgt gaactggaaa ctcgccgtac tctgcgcttt gaaatggcgg
     2641 gaaaaagcta ttttctcaaa tggcatcgcg gcacgaccct gaaagagata atcaaaaatt
     2701 tactctcatt gcggatgcca gtattaggcg cagaccgcga atggaatgcg attcatcgac
     2761 tgcgggatgt cggcgttgat actatgtatg gggtggcatt cggcgaaaaa ggcatgaatc
     2821 cgctgaccag aacctcgttt attattaccg aagatctgac accaaccatc agtctggaag
     2881 attactgtgc tgactgggcg accaacccac cagatgttcg cgtaaagcgt atgcttatta
     2941 agcgtgtcgc gacgatggtg cgcgatatgc atgctgcggg cattaaccat cgtgactgtt
     3001 atatctgtca tttcctgctg cacttgcctt tttccggtaa ggaagaggag ttaaaaattt
     3061 cggtaattga cctacaccgg gcgcagcttc gcacgcgcgt tccaggtcgt tggcgcgata
     3121 aagatcttat agggctttat ttttcttcaa tgaatattgg cctgactcag cgggatatct
     3181 ggcgctttat gaaggtgtat tttgccgccc cgcttaaaga cattctcaag caggaacaag
     3241 gactgctgtc gcaagcagaa gcaaaagcca caaaaatcag ggaaagaacg attcgaaaat
     3301 cgttgtaacc cgtaacttat ttttgccaaa att
//
LOCUS       DAZSNM010000297         3272 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZSNM010000297 DAZSNM010000000
VERSION     DAZSNM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.298"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(271..414)
                     /locus_tag="ACHW42_004022"
                     /pseudo
     CDS             complement(271..414)
                     /locus_tag="ACHW42_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            385..669
                     /locus_tag="ACHW42_004023"
     CDS             385..669
                     /locus_tag="ACHW42_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945613.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            complement(671..1243)
                     /locus_tag="ACHW42_004024"
     CDS             complement(671..1243)
                     /locus_tag="ACHW42_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945614.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(1329..1535)
                     /locus_tag="ACHW42_004025"
     CDS             complement(1329..1535)
                     /locus_tag="ACHW42_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8945615.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            complement(1921..2712)
                     /locus_tag="ACHW42_004026"
     CDS             complement(1921..2712)
                     /locus_tag="ACHW42_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945616.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
BASE COUNT          905 a          676 c          751 g          940 t
ORIGIN      
        1 catttcatca gtgtgacatt ccattgttta ctgataaagt tagccgataa ataatattgt
       61 tgagattatt gaaacggcag ttttcgccga ttcagtgcaa tggaagagat gttatatcat
      121 ctatttaagc aataagttta atacaagttt cagaagaggt gttggtgagc tgatgactga
      181 ttttcagagg gatatggaaa agtgtataga atcccataaa aaatcgatat tgttcagttg
      241 acgtatattt ctggaggatg actccagtca tcagaacata tgtttttcga tgaatgaccc
      301 aataattttt tcgtgaattt ccacagcacg tgaaaagtaa atcgtttttc ggtccagtcg
      361 tttgatgcgg gaacggagtg tcagatggtg gtgctcaata cgccgggtaa atatcaatac
      421 gatttgagga accatttgct ggttaatctc tttccgatag cattggatgc agaaataact
      481 acatccaatg taaataagca tcaattgcac agaagcaata tacctgcggc cctctccgga
      541 aattccggta tcactgacta cgtaccgttt tcagcggaac gaaatgcggc gatggtcatt
      601 tggcggacag ccatagatag ccgtccggct gtgtgtaacg atgaggaagg aaccccggga
      661 acgttatgaa tcagcaggcg tataacaggt actcgtcaat catcagttcc agctcgcgct
      721 tttcccgttt gcgcgccaca gcccagtttt caagctctgc atgtgattta tgctttaact
      781 cttcagtcca gggctgcatg gagtgggcac acatcagatc ataccagcac agccatatca
      841 gcgtgtggta gagatcacga tcgtcgaaac gccggagcag gctgttcagc ctgtttgcca
      901 gccggttttc acccagacga tgtagctcac tgagtaaaac gccgcccaga ttaaaatgtg
      961 taagcagcat gatttcatgg gtctgaatgc tggtcattaa cccttcctga atggcaggaa
     1021 tgaacggtgg tgttcccggg ttgacgacct gatagagtcc ctggggggcc accgaaatga
     1081 cggagccttt ttccggcaga aaaaagtgtg ccagggtcag acggtgataa ccatgaagat
     1141 gcggagaccc cattccatga gcgacgataa gcctcgtcac gcttgtcatc catttctgat
     1201 gctggatgag cagcaggtca cacctgtttt caagttgttg cataatattc tctcctttag
     1261 cgcgcagcaa aatgcctccg ggggccggat catgcctttg ctgcggtgga acggataacg
     1321 gtcaggaatt acggacggga ctgtgcaata cgcgcctgca gccaggcttc cacttcgctt
     1381 ttcagccagc gggagctacg gcccagtttg atggggggcg gaaaggctcc atgcttgatg
     1441 agtttataaa accacttatc ggtcaggccg gtcagctgag tgataaacga catgtcgacc
     1501 atctggtcat ccatcagtga aactggggta ttcatgagaa tgttcctccg gatgttgtta
     1561 atctcaaccg gcgttggtga tgaaaacgac gcaccacctt caccggcata acaaccgggg
     1621 agaaacgtaa aaaatgagta ggggaatatt cctgccagga gagaaactgt tgtgtacagg
     1681 caatacaggt tgtctgtttt gtcagtgaat ccggtataaa cggaagatga ttttgtgttg
     1741 ccggaatatg ggatggcgtt ttgccgtgac gggatcagta ttttcagtga tgattattac
     1801 tgcacagggg agtatcggag ataaaccacg ccctgtacct gccagagcgg tggtttgtat
     1861 ttactgaacg gaagataaag cagaggtaaa cactgtcgcg gtgaaggtag aaggctgaaa
     1921 ttattctctg taattacaaa caacagtgat cttatccggt ttttttaccg ttacgggttt
     1981 cagagggtca attttatata caggggtaat gagcacgccg gaatttatgg aaaaaagagg
     2041 gatccagcaa taaacgtccg gatatgtctg tgtctccgga ataccttttg cgccagagac
     2101 catcagatct gtaacgctca caaaaaatga ctgccgcaaa tcaaaaaagt actcgttata
     2161 tatcacatcc tttgccagca ggatgactgc attaccaaca gccgctttta caggtatttt
     2221 atcagatgga ttatcgtgat tgtatattgt tctttcgata taaataaaac aggcatattt
     2281 cgtcttctcc ccggatagct gcatctggta ttcgcaaatg tctttgatac accgactttc
     2341 aacccttcca atcatcttat cccggcgata gtgtctttta tgtttcagcg cgtacagaac
     2401 accatttgaa ctgtcaaact ccaggattct tctggtgaaa agtttgtttt cccagatttc
     2461 cagacaatac ttctctgcac tgacgacatt gcgtttgcga ggaagatccc gttgatacac
     2521 tccatttaat aagcgtgact gcagttcgcc tgcaagttga taataacagt taatatcatt
     2581 aaggacaggt gctgtggcgc tgagtaatgc gttgattacc ttagagcgcg aaagtttgtg
     2641 ggtatgacag tactcatcca caattcgcag cgtctcatca ctggtcatac tgaacgttat
     2701 acgtgttgtc atgatgaagt tttctgttaa tcatattggt tgagatgcat aacatttcat
     2761 aaaaaaacct gttaattcat gcaattaatt ttttaatgaa ttttatcctc aagtgatgga
     2821 aagcagtgtt actgccactg tactcagtat aactctatca cacagaatca cagaaaatga
     2881 agcattgctc agggggacac tcatacgagt tctctgaact gtgataacca gtatttttca
     2941 ttttgggtga tttgtttatc gttacagcta caggtagttc atgtaccata aaaaaataat
     3001 taaggttaat tagtattatt tacagaagta tggtcatgta aatgatcctg tgattatacc
     3061 ttgtaaatac accttataac ccaatcctgt agacggatct ctcataaaga tatactcata
     3121 gaagtatctg tgcagtgata ttctctctag tagtacttgt aactctgtcc ttacccctct
     3181 ctgctgtaat gtagtcccgc aacacctgaa tataaatctg taatgtacac actgactgta
     3241 atgttctttg ttaagtaatg tctgttattt tt
//
LOCUS       DAZSNM010000298         3259 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZSNM010000298 DAZSNM010000000
VERSION     DAZSNM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.299"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(89..616)
                     /gene="mobB"
                     /locus_tag="ACHW42_004027"
     CDS             complement(89..616)
                     /gene="mobB"
                     /locus_tag="ACHW42_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR8945617.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(598..1182)
                     /gene="mobA"
                     /locus_tag="ACHW42_004028"
     CDS             complement(598..1182)
                     /gene="mobA"
                     /locus_tag="ACHW42_004028"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR8945618.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1252..1521
                     /locus_tag="ACHW42_004029"
     CDS             1252..1521
                     /locus_tag="ACHW42_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR8945619.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1598..2584
                     /gene="srkA"
                     /locus_tag="ACHW42_004030"
     CDS             1598..2584
                     /gene="srkA"
                     /locus_tag="ACHW42_004030"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR8945620.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2601..3227
                     /gene="dsbA"
                     /locus_tag="ACHW42_004031"
     CDS             2601..3227
                     /gene="dsbA"
                     /locus_tag="ACHW42_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR8945621.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
BASE COUNT          830 a          779 c          795 g          855 t
ORIGIN      
        1 aaaggctatc cgtcaggatg gcctttttgc gttggtgaaa aaaatgccgg gtgcgacgct
       61 ggctgcgtct gatccagcct actcttgctt atccgttctg cttttgcatc cactccacca
      121 caaaatcagc cagcccctca acatcattaa tatccagtaa tgcgacatca agattaagcg
      181 gcacatcact ggctacagca ataacatgcc tgtctatcac taattcttca ggtcgatgtc
      241 cggctccatc gcgaaacagc acaatctttg cgatctcttc atgcttaaac ccttcgacca
      301 gaatcaaatc cagctttgag gtatccattc gacttgcgag aaaatgtaga tccagctctt
      361 cttcgtctgg tgtttccgtc atcaaggccc atcgttgctg gctggcaacg atggtttgta
      421 ccgcgccagc cttgcgcagc tcatagctat ctttgcctgg cttatcaaca tccatatcat
      481 ggtgcgtatg cttaatcagc cccggacgga tccctctggc gcataatgcc gggatcagtt
      541 ttttcaaaag cgtagttttt ccggtaccac tccacgcggc aaaggcgagt aacggtatca
      601 tcgtttttcc tgccatcggg caagctcctc tggcgtattc acgttaacaa atgcatcttt
      661 atgatcgctg aaatcaaccg catgaccgcc agccagacgc ataaatgcca ttacccggcg
      721 ttctcctgct tgcagatatt ccagtaataa aggctcaata gcgcggttta ccagagcaat
      781 agtcgggtga tcgcgttcac cgtcatggac ccacacgaca ggcgcatctt tgcgctgatg
      841 attaagccgg gcggctaaat catggggaat gtaaggcgta tcgcacggac aaaacaaaaa
      901 ccactcacca gcttcctgct gcattactga aagcattcct gccagagggc ctgggtaatc
      961 cgccagtgaa tcttcaatca ctttcagacc gcctgcctga tagatttcct gatgacgatt
     1021 agcattaacc acgacgtgag agagctgcgt cttaagcgcg tcagcgacat gttgccacag
     1081 cggtttgcca tttaagtcca gcaatccttt atctacgccg cccattcgtc tggctttacc
     1141 gcctgccagc acaacgcctg ttatcgtcgt cattagattc accgatatcg cctcttttat
     1201 tgtgggattg accctgctaa cgtgtctgtc tcaagagtaa ggagcattac tatgaaatgt
     1261 aaacgtctga atgaagttat tgaactcctc cagccagcct ggcaaaaaga gccagacttt
     1321 aacttgttgc aatttttgca gaaactggcg aaagagtcag gttttgacgg cgaactggcg
     1381 gatttgacgg atgacattct gatctatcac ctgaaaatgc gtgattccgc gaaagatgcg
     1441 gtgatcccgg gtttgcagaa agattatgaa gaagacttca aaacggcgct gttacgcgct
     1501 cgcggcgtaa ttaaagagta aaagcttgta agcggcgcca ccaaaatcat cgtgaaatga
     1561 tatccttcat cattcgtaat gttttccgga tgatgggatg aacaacagcg cttttacttt
     1621 ccagacacta cacccggata ccatcatgga cgctctgttt aagcaaggga tccgggtgga
     1681 ttccggtctt accccgctta acagctatga aaaccgtgtc tatcaatttc aggacgaaga
     1741 gcgtcgacgt tttgtcgtca aattttatcg ccctgaacgt tggacagccg atcaaatcct
     1801 cgaagaacat caatttgcgt tgcagctggt aaatgatgaa gttccggtcg cagcacctgt
     1861 ggcctttaac ggtcagactt tattgaatca tcagggattt tatttcgctg tttttccaag
     1921 cgtcggtggt cgccagttcg aagctgataa tatcgatcag atggaagcgg ttgggcgtta
     1981 tttagggcgt atgcaccaga cggggcgcaa acagcttttt attcatcgcc cgaccatcgg
     2041 tctgaatgaa tatctcattg agcctcgcaa gctgtttgag gacgctacac tgataccttc
     2101 cgggttgaaa gcggcatttc tgaaagcgac agatgagctg atcgccgccg ttacagcaca
     2161 ctggcgggaa gatttcaccg ttctgcggct acatggagac tgccacgccg ggaatattct
     2221 ctggcgcgat ggtccaatgt ttgttgatct ggatgatgca cgtaatggtc cagctattca
     2281 ggatttgtgg atgttgctca atggcgataa agcccagcag cggatgcaac tggaaactat
     2341 tattgaagct tatgaagaat ttagcgagtt cgacaccgct gaaatcggac tgattgaacc
     2401 tttacgcgcc atgcgtttgg tttattatct tgcctggcta atgcggcatt gggctgatcc
     2461 cgcgttcccg aaaaatttcc cgtggttaac cggggaagat tactggctac gacagacggc
     2521 gacttttata gaacaggcaa aagttctaca agaaccccct ttgcaattaa cacctatgta
     2581 ttaatcggag agagtagatc atgaaaaaga tttggctggc gctggctggt ttagttttag
     2641 cgtttagcgc atcggcggcg cagtatgaag atggtaaaca gtacactacc ctggaaaaac
     2701 cggtagctgg cgcgccgcaa gtgctggagt ttttctcttt cttctgcccg cactgctatc
     2761 agtttgaaga agttctgcat atttctgata atgtgaagaa aaaactgccg gaaggcgtga
     2821 agatgactaa ataccacgtc aacttcatgg gtggtgacct gggcaaagat ctgactcagg
     2881 catgggctgt ggcgatggcg ctgggcgtgg aagacaaagt gactgttccg ctgtttgaag
     2941 gcgtacagaa aacccagacc attcgttcag catctgatat ccgcgatgta tttatcaatg
     3001 caggtattaa aggtgaagag tacgacgcgg cgtggaacag cttcgtggtg aaatctctgg
     3061 tcgctcagca ggaaaaagcc gcagctgatg tgcaattgcg tggtgttccg gcgatgtttg
     3121 ttaacggtaa atatcagctg aatccgcagg gtatggatac cagcaatatg gatgtttttg
     3181 ttcagcagta tgctgatacg gtgaaatatc tgtccgagga aaaataattc aatgtaaatt
     3241 acataaagcc cgtgaatat
//
LOCUS       DAZSNM010000299         3258 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZSNM010000299 DAZSNM010000000
VERSION     DAZSNM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.300"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..442
                     /locus_tag="ACHW42_004032"
                     /pseudo
     CDS             1..442
                     /locus_tag="ACHW42_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            1026..1742
                     /locus_tag="ACHW42_004033"
     CDS             1026..1742
                     /locus_tag="ACHW42_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945622.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            1976..2104
                     /locus_tag="ACHW42_004034"
                     /pseudo
     CDS             1976..2104
                     /locus_tag="ACHW42_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            2294..2698
                     /locus_tag="ACHW42_004035"
     CDS             2294..2698
                     /locus_tag="ACHW42_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945623.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2761..3258
                     /locus_tag="ACHW42_004036"
                     /pseudo
     CDS             2761..3258
                     /locus_tag="ACHW42_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          909 a          675 c          749 g          925 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      481 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      541 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      601 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      661 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
      721 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
      781 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
      841 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
      901 attctgcaga gtattttgat atgataaata tgcattaaca atgatattat tgttaatttc
      961 ctagcatgaa aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt
     1021 ttcttatgaa tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat
     1081 gtgtagggtg ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc
     1141 catggtgggt tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca
     1201 tagtaatggg tatacttttt accggttggg ggattggtat tttatttttt cttcttataa
     1261 agaaaagaag taaaatacaa gacttgagtt tttttagact attttttgct attaccctac
     1321 tgtttttccc tgtaatattc gaacttttaa ttctcaaaca atatttcata cttccattat
     1381 cactttcatt cattattagt tcattggtta tttcaataat aattcgtata tatggacgga
     1441 tcttttctgt atcatgtttt tcagatatcc cttttgttag agaacaccgc attaagttaa
     1501 ttatggctgg ttttttggtt tatttttggt ttttttcctt tcttgttggc tggtataaac
     1561 ctcaattaaa aaaagaatat cagatgcttt gttataataa tagttggtat tatgttcttg
     1621 ctcgttatga ttcacggctc gttttatcta gttcatttaa agatgacagt aacagattct
     1681 taatatttaa tacagaacag tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt
     1741 aataatcctc tcccacaaag tgaattaaat gaatatataa tgcgttcgaa gtatatgtat
     1801 tgagctgtca tactgagaaa caaaccttag ggcagcacaa ttaaagccag aaacaactat
     1861 gcaagaaagt tactaaataa gtgctcggaa cagtaaaaca ctcagggggg actcagtccg
     1921 gattatgtaa tcagataaat tgccaaacat cacaaatcac cagccggact gagtgatgcc
     1981 gagcattgca ccaatacccc gtgatgaacg atgcctgatg cagaaagctc cctataaaac
     2041 aaagaacaaa aactacgacc ggcgattcac cgtcatactg atgctgcatc gtggtgacac
     2101 tgtctgtcac tttcatgttg actgaacaac tctgctgcga agaaggtgta ccgccggcgg
     2161 gatttgattg tccggcgtct agcttccact tccctgatgg aactgaaagt tgttatggcc
     2221 ggtcagcttg ccctgtccgc aggacatgag gtgctgtgac ggacagcacc agtaacggaa
     2281 agtgacaatg aaaatgaata ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt
     2341 acccgctgct gcaaaagaga ctttactggc tatgccacgt aattcaacag aacattgcta
     2401 tgcggagatt aatgtgcacg gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc
     2461 cgggggaaag agctgggtcg aatgtaacag tgactactat tactctgata aaccaccggg
     2521 agtacagata ctcggaaccc gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc
     2581 gacactgcat gtggctggaa gaggggttgt gtgtattaaa aatatatgca gtccacgtgg
     2641 gatgataatt cacaggatac ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc
     2701 ttacccagca atagtagaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac
     2761 atgacaaaaa cagtatcaac cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc
     2821 agtgaagccc tgaagcttgc tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc
     2881 ctgtatgaat ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct
     2941 gagcgtgaaa gtgaactgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg
     3001 ctatcctcca aaaggccgcg acatactttg cgcagcggct ggtatacgtg gtgtcagcgt
     3061 cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgcg
     3121 gcggccttta cccggtcaaa acagcgttac ggtgcaccac gcctgacgga tgaactgcgt
     3181 gctcagggtt accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcaggggctg
     3241 agggcaaagg cctcccgg
//
LOCUS       DAZSNM010000300         3256 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZSNM010000300 DAZSNM010000000
VERSION     DAZSNM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.301"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            14..1054
                     /gene="gatD"
                     /locus_tag="ACHW42_004037"
     CDS             14..1054
                     /gene="gatD"
                     /locus_tag="ACHW42_004037"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR8945624.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
     gene            1160..1933
                     /locus_tag="ACHW42_004038"
     CDS             1160..1933
                     /locus_tag="ACHW42_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR8945625.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2015..2914)
                     /gene="yegS"
                     /locus_tag="ACHW42_004039"
     CDS             complement(2015..2914)
                     /gene="yegS"
                     /locus_tag="ACHW42_004039"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR8945626.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT          868 a          722 c          811 g          855 t
ORIGIN      
        1 atcaggagtt tttatgaaat cagtagtgaa tgatactgat ggtatcgtgc gcgttgcaaa
       61 aagcgtcatt cctgaaatta aacatcagga tgaggtgcgg gtaaaaattg ccagctcggg
      121 cttatgtggt tccgatttac ccaggatatt taaaaatggt gcacattatt atccaataac
      181 gttaggccat gaatttagcg gctatattga tgcggtggga tccggtgttg atgatttaca
      241 ccctggcgat gcggttgcct gtgtgccgtt attaccctgt tttacttgtc cagagtgttt
      301 gaaagggttt tattcccagt gcgcaaaata tgattttatt ggctcgcggc gtgatggtgg
      361 atttgctgaa tatattgtcg ttaagcgaaa aaatgtcttt gctctaccca cggatatgcc
      421 tattgaggat ggggctttta ttgagccgat taccgttggc ctgcatgctt ttcatttagc
      481 gcaaggttgt gagaataaaa acgttattat tattggtgcc ggaaccattg gcctgctggc
      541 tattcagtgc gctgtcgcgc tgggagcaaa gagtgtgacg gcgatcgaca ttagctcaga
      601 aaaactggca ctggcaaaat ctttcggtgc gatgcaaaca tttaacagta gcgaaatgag
      661 cgcgccgcaa atgcagagcg ttttacgcga actgcgcttt aatcagctta tcctcgagac
      721 ggctggcgta ccgcaaactg tcgaactggc ggtagagatt gccggtcctc atgcccaact
      781 ggcgctggtg ggcacgttgc atcaggatct gcatttaaca tcggcaacgt ttggcaaaat
      841 attgcgtaaa gagctgacgg ttatcggcag ttggatgaac tattccagcc cttggccggg
      901 gcaggagtgg gaaacggcga gccggttgct gacagaacgt aagttaagcc tggagccatt
      961 aatcgctcac cgtggaagct ttgaaagctt cacccaggcg gtgcgtgaca tcgctcgtaa
     1021 tgccatgcag ggcaaagtgt tgctcattcc atgaaaccgc gggccagcgt gatgctggcc
     1081 cggtattgtg caaaacagat cattcaccaa tggtccccct tcgtttacac tagccacaat
     1141 tgaatatggg taaatgacga tgaattcatt cgagcgaagg aataagatca tccaattagt
     1201 gaatgaacag ggaaccgtgc ttgttcagga tctggcggga gtatttgctg cctcggaagc
     1261 gacaatccgt gccgatttgc gctttctcga acaaaaaggc gtggttacgc gctttcatgg
     1321 cggtgcggcg aaaataatgt ccggtaatag tgaaaccgag acccaggaag tcgggtttaa
     1381 agagcgattt cagctcgcca gcgcgccaaa aaacagaata gcgcaggcgg cagtcaaaat
     1441 gatccatgaa gggatgaccg ttattctcga cagcggaagt accacaatgc ttatcgccga
     1501 aggattaatg accgctaaaa atatcacggt gattaccaac agtctcccgg cagcgtttgc
     1561 cctttccgaa aataaagaca ttactctggt cgtctgtggt ggcacggtcc gccataaaac
     1621 gcgctcgatg catggttcca ttgccgagcg ttcattgcag gatattaatg ccgatttaat
     1681 gtttgtcggt gctgatggta ttgatgcggt taatggtatt acgaccttta atgaaggtta
     1741 ttcgattagc ggggcgatgg tcaccgccgc aaataaagtg attgccgttc tcgattcatc
     1801 gaaatttaat cgtcgcggtt ttaatcaggt actgccaatc gaaaaaattg acattattgt
     1861 taccgatgat gctgtgtcag aggtggataa attggcattg cagaagacac gggtaaaatt
     1921 aattacggtg tagtattgtt ctgtatgccg gataaggtgt ttacaccgca tccggcatta
     1981 cggattctcc ctggttcaag agagaatagt ttgattaacg caataatgga caatctggtg
     2041 gtaatcgaca acgcaacgcc gccggaagta tttcaatatg gaagttttgc ccactcaacg
     2101 gttcgccatc aagattaaaa gtgatttcgt gtggggcttg tatatcaaac cacgacgaag
     2161 cgccttcgat aatattcggg ttatcttcgt cagattttaa ggttgatacg agagccggaa
     2221 gaatttcatc gccggtaaaa atgcgcagtt gcagcaagcc atcgttaatt aacgcgttcg
     2281 ggcacaattg ttgaccgcca ccggcctgac gcccgttacc aataccaatg accagggcgt
     2341 caccttgcca gtgaaagttt tcaccgcgga tttcacaacg gtccggttgc agagtatcca
     2401 tgcgcattaa gccatgaatg atgtaagaga cgccacccag cgcggctttt aatttttccg
     2461 gcgtttctgt ggtaatacgc gtcccaaatc cgcctgtcgc catattaata aaacaggttt
     2521 gtttgttgac ctgcgccata tctatcgcaa tggcattacc ggcaattgcc agtttcagcg
     2581 ccttatccag tgcctcagga atccctacac tggtggcaaa atcattggcg gttcctaatg
     2641 gcaaaattcc cagcgcgggt atgtcatccc cctcacactg aatcaacgcc gtagaaactt
     2701 cattaatggt gccatcgcca ccaccggcaa tcaccgttgc gacgcccaac ttccgggcct
     2761 cctctacaaa tcgtgcggca tcacctttct cccaggtgac ccgcacatgg atcgtcattc
     2821 cttcctcacg caacagcata attgcttcgc gcaagggtag attgtcagta cttttaccat
     2881 taagaatcag taagctggcg ggaaattctg ccatgatcgt atgtgccttt atgattggtc
     2941 tgtagatagt gtagagcaga aaacaaaagg tggagtcaga acaggatgaa agtcggagga
     3001 taaaaagtca gttcaagtat ggaggattca gtggttacca ccaggagctt gcaatgggga
     3061 cagcgaatac taacggctgc caccgataaa tttcaaaaaa gagcatatac ctaatattca
     3121 actaaacagt ggcatcttca atataatata ttaaagcccc catggagtta ccctgaaggg
     3181 cctcaatgtc cgtaattcct acttatgtag gaaatgttgt acagaacatt tattataatc
     3241 ctattcaatt ataata
//
LOCUS       DAZSNM010000301         3225 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZSNM010000301 DAZSNM010000000
VERSION     DAZSNM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.302"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..3087
                     /locus_tag="ACHW42_004040"
     CDS             <1..3087
                     /locus_tag="ACHW42_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR8945627.1"
                     /translation="TFSINFDDSVDKTAETDEEREARYAALQQEWRQYLYDSERLLKS
                     VAGDWIEKPEQYELAEHGYIVKTAQSGGASSHILSLYDHLLVCNKDVPLFNRFASREV
                     HAAESLLAPGAKFSDRLGHSGDKFPLAKAQRDALSHFLDARHGDILAVNGPPGTGKTT
                     LVLSIIATQWARAALEKSEPPVIIATSTNNQAVTNIIEAFGKDFSQGSGAMAGRWLPE
                     LKSFGAYFPSSSRKAEAAKKYQTEDFFNQVESKEYVEDALLFYLEKAKAAFPGKECSS
                     PEKVIELLHGQLAAKSEQLIRLNATWQTLSQIRAARELIANDIEQYLDNLNKLLSGQE
                     QKVTLLKSAKTEWKKYRAGESLIYSLFSWLPAVRNKRQYQIQLFLEDKLGALIAGNQW
                     SDPETIERNIDGLLNSAEREQTTYRQQIDSAHEIVLKEQQAVQEWQRLAFDLGYEGDE
                     ELSFSQADELADTQIRFPAFLLTTHYWEGRWLMDMASIDDLQDEKKKKGAKGVTARWQ
                     RRMKLTPCVVMTCYMLPGNMQISEHKGQRKFEKSYLYDFADLLIVDEAGQVLPEVAAA
                     SFALAKKALVIGDTEQIPPIWSIAPAIDVGNMLAEKILSGSTQEEITEKYTAIADLGK
                     SAASGSVMKIAQFASRYQYDPELARGMYLYEHRRCYDNIIGYCNTLCYHGKLLPKRGR
                     EESNLMPAMGYLHIDGKGELASSGSRYNLLEAETIAVWLAENQQNIEAHYGKSLHEVV
                     GIVTPFSAQVSTIKQVLGKQDISTGTNEKSLTVGTVHSLQGAERAIVIFSPVYSKHED
                     GGFIDSDNSMLNVAVSRAKDSFLVFGDMDLFEVQPASSPRGLLAKYLFESEKNALSFD
                     YKERKDLKTAGTKIYTLHGVEQHDNFLNQTFENTSKHITIISPWLTWQRLEQTGFLDS
                     MIAACSRGINVTIVTDRSYNTEHNDFEKRKEKQQNFKAALEKLNALGIATKLVNRVHS
                     KIVIGDDGLLCVGSFNWFSATREARYERYDTSMVYCGDNLKGEIEAIYNSLERRQV"
BASE COUNT          909 a          668 c          837 g          811 t
ORIGIN      
        1 acgttctcta tcaactttga tgacagcgtt gataagactg ccgaaacgga tgaagaacgg
       61 gaagcacgat atgccgcctt gcagcaggag tggcgtcaat atctgtatga ctcagagagg
      121 ctactgaaga gcgttgccgg cgactggatt gaaaaacctg agcaatatga actcgctgag
      181 cacggttata ttgttaaaac ggctcaatct ggcggtgcca gttcccatat cctttctctt
      241 tatgatcacc tgcttgtttg caataaggat gtgccgctct tcaatcgctt cgcctcgcga
      301 gaggttcatg ctgcagagtc tttgctggcc ccaggagcaa aattcagcga caggcttgga
      361 cactccggag ataagtttcc gctggcaaag gctcagcgcg atgccttaag ccattttctg
      421 gatgcaagac atggcgatat ccttgctgtt aatggccctc cgggaaccgg aaaaaccacg
      481 ctggtgcttt ctatcatcgc cacgcagtgg gccagagcgg ctctcgaaaa atctgagcct
      541 ccggttatta tcgcgacttc aacgaataac caggctgtaa cgaacattat tgaggcattc
      601 gggaaagact tttcgcaagg ttcaggtgcg atggccgggc gatggttgcc agagctgaaa
      661 agcttcggtg cttattttcc ctcaagcagt cgtaaagctg aggcagccaa aaaatatcaa
      721 actgaagatt tcttcaacca ggttgagtca aaagagtatg tagaggatgc actgctgttt
      781 tatctggaaa aggctaaggc agcctttcct ggaaaagagt gttcatcccc tgaaaaggtc
      841 attgaactct tgcatggtca gttggcagca aaatctgagc aactgataag actgaacgca
      901 acatggcaaa cgttaagcca gattcgggct gcgcgtgagc ttattgctaa tgatattgag
      961 caatatctcg ataatttaaa taaattactt tccggacaag aacaaaaagt cactctactg
     1021 aagagtgcta aaacggaatg gaaaaaatat cgcgccggtg aatcactgat ctattcatta
     1081 ttttcctggc tcccggcggt tcgcaataag cgacagtacc aaatacagct gtttctcgaa
     1141 gataaattag gcgcgctgat tgcaggaaat cagtggtctg atcctgaaac tatcgaacgt
     1201 aatattgatg ggctgctcaa ttccgctgag cgcgagcaaa caacataccg gcagcagatt
     1261 gactccgccc atgaaatcgt tcttaaagaa cagcaggcgg ttcaggagtg gcagaggctg
     1321 gcatttgatt tagggtatga gggcgacgag gaactgagct tctcacaggc cgatgaactg
     1381 gctgatacgc agattcgctt ccctgcattt ttactgacga ctcactactg ggaaggtcgt
     1441 tggctgatgg atatggccag cattgatgat ctgcaggatg agaagaagaa aaaaggtgct
     1501 aaaggggtaa ccgcccgttg gcaacgtcga atgaaactca cgccatgtgt ggtgatgaca
     1561 tgctatatgc tgcccggtaa tatgcagata agtgagcaca aaggacaacg taaattcgag
     1621 aaaagttatt tgtatgattt tgccgattta ctcattgtcg atgaagccgg gcaggtgctt
     1681 cctgaagtgg ctgctgcctc gtttgcatta gctaagaagg cattagtgat tggcgatacg
     1741 gagcagatcc cgccaatatg gagtattgct cctgcgattg atgtcggtaa catgctggcg
     1801 gaaaaaattc tgtctggcag tacgcaagaa gagattaccg agaaatatac ggcaatcgca
     1861 gaccttggta aaagtgccgc atctggcagc gttatgaaaa tagcgcagtt tgcttcgcgc
     1921 tatcaatatg atcccgaact ggctcgtggt atgtacctat atgaacaccg ccggtgctac
     1981 gacaatatta ttggatactg taatacgctc tgctatcacg gtaagttgtt gcctaaaaga
     2041 gggcgtgaag agagcaattt aatgcccgca atggggtatc tccatattga tggtaaagga
     2101 gagctggcaa gtagtggaag tcgatataat ttgcttgagg ctgaaacgat agcggtctgg
     2161 ttggcagaga accagcaaaa tattgaagcg cattacggta aatcgcttca tgaagttgtc
     2221 ggtattgtga cgccttttag cgctcaggta tccactatca aacaggtgct gggcaaacaa
     2281 gatatcagta caggcacgaa tgaaaagtcg ctcacagtgg gcaccgtgca ctctcttcag
     2341 ggagcggaaa gagcgattgt gatattctcg ccagtctatt caaaacatga agacggcggg
     2401 tttattgata gcgataacag catgctgaat gttgcagtct cccgtgcgaa ggacagtttt
     2461 ctggtcttcg gcgatatgga cctgtttgag gtccagccag cctcatcgcc acggggatta
     2521 ctggcaaaat acctctttga gtcagagaag aatgcgctct cttttgatta taaagagcgt
     2581 aaggatttaa aaaccgccgg gaccaaaatc tacacacttc atggtgtgga gcaacatgat
     2641 aatttcctga atcagacatt tgaaaatacc agtaaacaca tcacgataat ttctccatgg
     2701 ctgacctggc aaaggctgga gcaaaccggt tttcttgatt ccatgattgc ggcgtgttca
     2761 cgtggaatta acgtcacgat agtcactgac agaagctaca acactgaaca taatgatttt
     2821 gagaagcgaa aagagaagca gcagaacttt aaagcggcgc tggagaaact gaatgcgctg
     2881 ggtattgcta caaagctggt aaaccgtgtt catagcaaaa ttgttattgg tgatgatggt
     2941 ttgctgtgtg tgggatcgtt caactggttt agtgcgacac gggaagcgcg atatgaacga
     3001 tacgatacat caatggttta ttgcggtgat aacctgaagg gtgagattga ggctatttat
     3061 aatagtcttg agaggcgtca ggtttagagc aggaatggtg agtagccagc ttaccgatcg
     3121 ttttcgagcg taagatggct gaatggaatg gctattattg cacagtcctt aattataaca
     3181 ttcataccga catgattatc ttctgtccgg aagaatcaga ggctc
//
LOCUS       DAZSNM010000302         3213 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZSNM010000302 DAZSNM010000000
VERSION     DAZSNM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.304"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..342)
                     /locus_tag="ACHW42_004041"
     CDS             complement(<1..342)
                     /locus_tag="ACHW42_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945628.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
     gene            692..1714
                     /locus_tag="ACHW42_004042"
     CDS             692..1714
                     /locus_tag="ACHW42_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFR8945629.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1714..2694
                     /locus_tag="ACHW42_004043"
     CDS             1714..2694
                     /locus_tag="ACHW42_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFR8945630.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2691..>3213
                     /locus_tag="ACHW42_004044"
     CDS             2691..>3213
                     /locus_tag="ACHW42_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8945631.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDLGH"
BASE COUNT          724 a          849 c          765 g          875 t
ORIGIN      
        1 gagaatgtta tcattgctac accctgtgct taataactgc aactctccgc cgttcagcga
       61 tgctttgata attctgttcc catagatatc gttatcaaaa taaagcgtaa atatcatatt
      121 tccgtaactg ttatcatctc tttttttgca cgtccgaata aacgcattca gtaaattact
      181 ctctctaaga aaataaatat cttcattgtt gttaatgaca ttaataacgt cgttcttaaa
      241 atcctcaaag tcttgttgcg gtaacttctg gttacgctgc ttactatcag caagcggtga
      301 atccggacga caacaacata ggttttgtat aacccccagc attcttttca cctccgttaa
      361 cagtatattc acgacactga aacgagagaa tgctgataat tttctcggcg agatatcaaa
      421 aatcgctcat tataaaacaa gtaacttcaa cgtcgcgtaa ccgtggacga taaaaaaaca
      481 ataatgtcat tcatatttaa ataatttagc cttaaatgat ttaaataata ttagccacct
      541 gataaataca tttttaatga tagagagggt caacaggctt gcggaagtcg tcacatcttc
      601 atgttttcaa tagctaaact ttgatattga acatgatttt tatcgatata tatgaaagta
      661 tatatcgatt ttacttttca ggatgttcac catgcccata accagacgga cgtttgcgca
      721 ggcacttgca tctaccctgc tcctgcagtc tctaccgtcc ttttcacaaa cagttaatcg
      781 ttttgcttcg caatctcttc ccgaagccca aaacattaca cgcatcgtca gcgcaggcgc
      841 gcccgccgat ttgctcttgc tggctgtcgc gccagaaaaa atggtcggtt tttcctcttt
      901 tgattttgcc cgtcaggcat taattccatt gccagagcac attcgccagt tacccaggct
      961 gggacgactc gccgggcgcg ccagcacact atcgctggaa gggcttatgg cgttacatcc
     1021 cgatttggtc gttgattgcg gcaatacgga tgaaaccttg atctcccagg cacggcaggt
     1081 tagcgaacag acacaaatcc cctggttatt gcttaacgga aaactggcgc aatcagcaga
     1141 acagttaaca acgcttggca aaacgttagg cgaagagcac cgcgccgcag aacaagccaa
     1201 tctcgccagc cacttcgttg gtgaagctca ggcattcgcc acctcacccg ccgctaacct
     1261 ccgcttttat gctgcgcgcg gtccccgagg gctggaaacg ggcttacagg gttcgttgca
     1321 taccgaggcg gcggaattat taggtttgca caacgtcgcg caaatagccg atcgccacgg
     1381 cctgacacag gtttccatgg aaaatctcct gcgctggcag ccggatatta ttctggttca
     1441 ggaggccgtt actgcagatt ttattcgtcg tgatcctctc tggcagggcg tgaaagccgt
     1501 tgcggaacaa cgcatcttat ttttaagtgg cttgcccttt ggctggctcg atgccccgcc
     1561 gggaatcaac cgtcttctgg gattacgcag acttcacgcc tggctggatc ccgccatcaa
     1621 tcgccagttt aaaagtgaca tgcagcatta cgcccaactg ttctggcatt gttcactcag
     1681 tgatgccgac tatcaaaaat tggtggcgag ctaatgagaa tcgtaaacgg atgtattttg
     1741 ctgctggcag ctatcagtat cacttttgcc gccgtaagtg gggcttatca tctcgatatg
     1801 cagcaactgc tggcgctaat tcttcgtcaa gaaaatgagt ctgtgcagga acaaattgtc
     1861 ttctggcaaa tccgcgtgcc gcgtattctt gctgcgctgt tactgggcgc agcgctggca
     1921 ggagccggaa ccacctatca gggaatgttg cgtaatccct tagtttcacc ggatatttta
     1981 ggcgtctccg ccggtgccgg acttggtgcc tgcgcggcaa tcctgtgggg attacccatt
     2041 gtttacatcc agctgtacgc attttgcggc gggctgatgg tggtcgcggg cgtctggttg
     2101 attacgcgtc gggtgacccg ccacgatccg atcttaaccc tggtgctggt aggcattgcg
     2161 ttaggaacgt tgtgcggagc ggggatatcg ctaattaaaa cgctggccga tccttacacc
     2221 cagttaccgt caattacctt ctggttgctg gggggactgt ccaccgtgac cttacgcgat
     2281 ctctgctatg ctgcaccgat cattctcata ggctccttgc ccctgttttt tctccgctgg
     2341 cgcatgaatt tactcacgct ttccgacgac gaagcccgtt cgctgggact gaacgtgacg
     2401 cgtctgcgct ttggattgat tgtctgcgcc acactaatca ccgccagcac ggtagcaata
     2461 gcgggcataa ttggttggat cggtctggtc gttccgcaca ttgcccgctt gttgaccggt
     2521 cataaccatc agcagctgtt gcctatggca atgtgtactg gcgctattct gctcctgctt
     2581 accgatacgc tggcgcgcag cattggaaca acagaaatcc cacttggcat tctgaccgct
     2641 tttgttggcg caccgttttt cctgcttttg ttgttacgcg gaggtcgcca gtgactttgc
     2701 ttgccgttcg tcatgcgtct ctgggttact cccgccatcc agtgctacgc gacgtgtcct
     2761 tcaccctgtc gcaaggaaca atttgttgtc tgcttggcgc gaatggttgc ggcaaaacca
     2821 cactcatgcg ctccattctt ggcgttatac cgttattaaa aggcgagatc ctactggata
     2881 gcctctctgt gcagacactc agccatcgcc agcgcgcaca ggctattgcc tgggtgccgc
     2941 aggcgcacga cggtattttt gctttcagtg tgctggatat ggtgttgatg ggcctggccc
     3001 ccaccatagg cgctttttcc gttcccggta agcaagaacg cctgaaagcc acggagcaac
     3061 tggaaaagct gggcattttg catctggcga cacgacgctg gaatacgctt agcggcggtg
     3121 aacgtcagct ggctttaatc gcccgtgcac tcgtccaaca accgcgcttg ttgttactgg
     3181 atgagcccgc ctccagcctc gatctcggtc atc
//
LOCUS       DAZSNM010000303         3179 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZSNM010000303 DAZSNM010000000
VERSION     DAZSNM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.306"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHW42_004045"
     CDS             418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHW42_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR8945632.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(1772..1906)
                     /locus_tag="ACHW42_004046"
     CDS             complement(1772..1906)
                     /locus_tag="ACHW42_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR8945633.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2552..2722)
                     /locus_tag="ACHW42_004047"
     CDS             complement(2552..2722)
                     /locus_tag="ACHW42_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR8945634.1"
                     /translation="MNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSI
                     QIRFEAESETGT"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAZSNM010000304         3178 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZSNM010000304 DAZSNM010000000
VERSION     DAZSNM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.307"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            159..1262
                     /gene="proB"
                     /locus_tag="ACHW42_004048"
     CDS             159..1262
                     /gene="proB"
                     /locus_tag="ACHW42_004048"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR8945635.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1274..2527
                     /gene="proA"
                     /locus_tag="ACHW42_004049"
     CDS             1274..2527
                     /gene="proA"
                     /locus_tag="ACHW42_004049"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR8945636.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2642..2717
                     /locus_tag="ACHW42_004050"
     tRNA            2642..2717
                     /locus_tag="ACHW42_004050"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2675..2677,aa:Thr,seq:cgt)
     gene            complement(2732..>3178)
                     /locus_tag="ACHW42_004051"
     CDS             complement(2732..>3178)
                     /locus_tag="ACHW42_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8945637.1"
                     /translation="ESKSNRAIGVALNETACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
BASE COUNT          749 a          807 c          875 g          747 t
ORIGIN      
        1 attaaatctg taataaaagc gtaaacaact gccgctacgc ttgctgatcc cgcgcaacaa
       61 aacgccatgc tttgctcgca gatggttggc aaccgacgac agtcctgcta aaacgttcgt
      121 ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc cagacgctgg
      181 tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac cgtgcccata
      241 tcgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg attgttattg
      301 tgacgtcggg cgcgatcgcc gccggacgtg agcacctggg ttacccggaa ctgccagcga
      361 ctatcgcctc gaaacaactg ctggcggcgg tagggcagag tcgactgatt caactgtggg
      421 aacagctgtt ttcgatttat ggcattcacg tcgggcaaat gctgctgacc cgtgctgata
      481 tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg ctcgataaca
      541 atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt aaggtcggcg
      601 ataacgataa cctttctgcg ctggcggcga ttctggcggg tgccgataaa ctgttgctgc
      661 tgaccgatca aaaaggtttg tataccgctg atccgcgcag aaatccgcag gcagaactga
      721 ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgccggtgac agcgtttcag
      781 gcctcggaac tggcggcatg agtactaaat tgcaggccgc tgacgtggct tgccgtgcgg
      841 gtatcgacac cattattgcc gcgggcagca agccgggcgt tattggtgat gtgatggaag
      901 gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac cgtaaacgct
      961 ggattttcgg tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca actgccgcca
     1021 ttctggaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact ggcaatttct
     1081 cgcgtggtga agtcatccgc atttgcaacc tcgaaggtcg cgatatcgcc cacggtgtca
     1141 gtcgttacaa cagcgatgca ttacgccgta ttgccggaca ccactcgcaa gaaattgatg
     1201 caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg attacccgtt
     1261 aaggagctgg ccgatgctgg aacaaatggg cattgccgcg aagcaagcct cgtataaatt
     1321 agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg atgaactgga
     1381 agcacaaagc gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg cgcgagccaa
     1441 tggccttagc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc tgaaaggcat
     1501 tgccgacgat gtacgtcagg tgtgcaacct cgccgatccg gtggggcagg taatcgatgg
     1561 cggcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg gggttattgg
     1621 cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt gcctgaaaac
     1681 cggtaatgcg gtgatcctgc gtggtggtaa agaaacctgt cgcactaacg cggcaacggt
     1741 ggcggtgatt caggacgccc tgaaatcctg tggcttaccg gcgggtgccg tgcaggcgat
     1801 tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat acatcgacat
     1861 gctgatcccg cgtggtggcg ctggtttgca taaactgtgc cgtgaacagt cgacaatccc
     1921 ggtgatcaca ggtggtatag gcgtatgcca tatttacgtt gatgaaagtg cagagatcgc
     1981 tgaagcatta aaagtgatcg tcaacgcgaa aactcagcgt ccgagcacat gtaatacggt
     2041 tgaaacgttg ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat taagcaaaca
     2101 aatggcggaa agcggcgtga cattacacgc agatgcagct gcgctgacac agttgcaggc
     2161 aggccctgcg aaggtggtgg cggttaaagc cgaagagtat gacgatgagt ttctgtcatt
     2221 agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata ttcgtgaaca
     2281 cggcacacaa cactccgatg cgatcctgac ccgcgatatg cgcaacgccc agcgttttgt
     2341 taacgaagtg gattcgtccg ctgtttacgt taacgcctct acgcgtttta ccgacggcgg
     2401 ccagtttggt ctgggtgcgg aagtggcggt aagcacacaa aaactccacg cgcgtggccc
     2461 aatggggctg gaagcactga ccacttacaa gtggatcggc attggtgatt acaccattcg
     2521 tgcgtaaata aaaccgggtg atgcaaaagt agccatttga ttcacaaggc cattgacgca
     2581 tcgcccggtt agttttaacc ttgtccaccg tgattcacgt tcgtgaacat gtcctttcag
     2641 ggccgatata gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg taggttcgac
     2701 tcctattatc ggcaccattt aaatcaataa gttacacatc attagtacct tccttatttc
     2761 ttgactggga caaatttggg accgatgggt tcagaatcaa gtctatttgc cgtgcgtgtt
     2821 cggtaaggtg attaggtgcg aggtgagcat atcgacgaac catttcgata gactcccagc
     2881 ctcccatttc ctgtaacact gataacggga ctccggcttg aaccagccaa cttgcccagg
     2941 tgtgtctcaa gtcgtgaaat ctgaaatcat caataccagc ccgtctcagc gccgctttcc
     3001 aggctgtgtt tgcgtcatac cgcatcttcc ttactgttgg cgctttcgtt ccgtctggtt
     3061 tggtacagct ttccttgtac acaaataccc aacggtgatg atttccgatt tgttttttca
     3121 atacgcgaca tgcagtttca ttcagcgcaa cgccaattgc gcggtttgat ttactctc
//
LOCUS       DAZSNM010000305         3148 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZSNM010000305 DAZSNM010000000
VERSION     DAZSNM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.308"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..471
                     /locus_tag="ACHW42_004052"
     CDS             <1..471
                     /locus_tag="ACHW42_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFR8945638.1"
                     /translation="KAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDENKMHL
                     FTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQKITLNNNIV
                     DDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLSRK"
     gene            746..1942
                     /gene="kbl"
                     /locus_tag="ACHW42_004053"
     CDS             746..1942
                     /gene="kbl"
                     /locus_tag="ACHW42_004053"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR8945639.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            1952..2977
                     /gene="tdh"
                     /locus_tag="ACHW42_004054"
     CDS             1952..2977
                     /gene="tdh"
                     /locus_tag="ACHW42_004054"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR8945640.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
BASE COUNT          762 a          699 c          839 g          848 t
ORIGIN      
        1 aaagctatca acatgggttt agtgaaaacg cctcttgttg cgtggataga ttttggctat
       61 tgccgtaagc caaatgtaac tcgaggattg aaaatttggg atttcccatt tgatgaaaat
      121 aagatgcatc ttttcactat aaaaaaaggg cttactgtta catctcaaca acaggctttt
      181 gatttcatga ttggcaatca tgtatatatt attgggggcg caattgttgg ttcacagcat
      241 aaatggaaag aattctataa attagttctg gaaagtcaaa aaataacact aaacaataac
      301 attgtggacg atgaccaggg catttttgtc atgtgctatt acaaacgttc cgatctcttc
      361 aacctaaact atctggggag aggaaaatgg ttcgatttgt ttcgctgctt caggagtaac
      421 actttagggg caaaaatgca ggcgctgaga attttccttt caagaaaata ggtttccctc
      481 cattagtggg tagcgttttg atgccaactg agataggaaa aatgatgctt tggcatcatt
      541 ttttcttttt atatcaatgc agtaaaatca ctcatcatta tgctcagcca aaactgtaca
      601 gtttttctac tatctgtagg tgttgagaga catcaagata cacgttattt atcctgaatt
      661 ttgcagaagt gttaacgcat catctcgtcg cgacctataa gtttgggtaa tatgtgctgg
      721 aatttgccct gtctggagaa tcgcaatgcg tggagaattt tatcagcagt taaccaacga
      781 tctggaaacc gcacgggcgg aagggttgtt taaagaagag cgcattatta cgtctgcgca
      841 gcaagcagat atcaccgtgg ctgatggaag ccacgtcatt aacttttgtg ccaacaacta
      901 tctcgggctg gcgaatcatc cggatctgat tgcggcagca aaggcgggaa tggattctca
      961 cggtttcggc atggcttcgg tgcgttttat ttgcggcact caggacagcc ataaagagct
     1021 tgaacaaaaa ctggcggcat tcctggggat ggaagatgcg attctctact cttcctgctt
     1081 tgatgctaac ggtggcctgt ttgaaacgct gctgggcgcg gaagacgcca ttatctccga
     1141 cgcactgaac cacgcgtcta ttattgatgg tgtgcgtctg tgtaaagcta aacgctatcg
     1201 ctatgccaac aacgatatgc aggagcttga agctcgtctg aaagaagcgc gtgaagccgg
     1261 tgcgcgtcat gtgctgattg ccaccgatgg tgtgttctcg atggacggcg tgattgccaa
     1321 tctgaagggc gtttgcgatc tggcagataa atacgatgcc ctggtgatgg tagacgactc
     1381 ccacgcggtc ggttttgtcg gtgaaaatgg tcgtggttcc catgaatact gcgatgtgat
     1441 ggggcgtgtc gacatcatca ccggcacgct cggtaaagcg ctgggcgggg cttctggtgg
     1501 ttataccgcg gcgcgcaaag aagtggttga gtggctgcgc cagcgttctc gtccgtacct
     1561 gttctccaac tcgctggcac cagcaattgt cgccgcttcc atcaaagtgc tggagatggt
     1621 tgaagcgggt agcgagttgc gtgaccgtct gtgggcgaac gcgcgtcagt tccgtgagca
     1681 aatgtcggcg gcgggcttta ccctggcggg agccgatcac gccattattc cggtcatgct
     1741 tggtgatgcg gtagtggcgc agaaatttgc ccgagagctg caaaaagagg gcatttacgt
     1801 caccggtttc ttctatccgg tcgttccgaa aggtcaggcg cgtattcgta cccagatgtc
     1861 tgcggcgcat acccctgagc aaattacgcg tgcagtagaa gcattcacgc gtattggtaa
     1921 acaactgggc gttatcgcct gaggatgtga gatgaaagcg ttatccaaac tgaaagcgga
     1981 agagggcatc tggatgaccg acgtccctgt accggaactc gggcataacg atctgctgat
     2041 taaaatccgt aaaacagcca tctgcgggac tgacgttcac atctataact gggatgagtg
     2101 gtcgcaaaaa accatcccgg tgccgatggt cgtgggccat gaatatgtcg gtgaagtggt
     2161 aggtattggt caggaagtga aaggcttcaa aatcggcgat cgcgtttctg gcgaaggcca
     2221 tatcacctgc ggtcactgtc gcaactgtcg tggcggtcgt acccatctgt gccgcaacac
     2281 cattggtgtc ggtgttaacc gtccgggctg cttcgccgaa tatctggtga ttccggcgtt
     2341 caacgctttc aaaatccccg acaatatctc cgacgacctg gcttccattt ttgatccctt
     2401 cggtaacgcc gtgcataccg cgctgtcgtt cgatctggtg ggcgaagatg tgctggtttc
     2461 tggtgcaggt ccgataggta ttatggctgc ggcggtggcg aaacacgttg gtgcacgcaa
     2521 tgtggtgatc accgatgtta acgaataccg ccttgagctg gcgcgcaaaa tgggtatcac
     2581 ccgtgcggtt aacgtcgcga aagaaaacct taatgatgtg atggctgaac tgggcatgac
     2641 cgaaggcttt gatgtcggtc tggaaatgtc cggcgcgccg ccagcgtttc gtaccatgct
     2701 cgataccatg aaccacggcg gtcgtattgc gatgctgggt attccaccgt ctgatatgtc
     2761 tatcgactgg accaaagtga tctttaaagg cttgttcatt aaaggcattt acggtcgtga
     2821 gatgtttgaa acctggtaca agatggcggc gctgattcag tctggcctcg atctctcgcc
     2881 gatcattacc catcgtttct ctatcgatga tttccagaag ggctttgacg ctatgcgttc
     2941 gggccagtcc gggaaagtta ttctgagctg ggattaacac gaacaagggc tggtattcca
     3001 gcccttttat ctgaggataa tctgttaatt atgtaaaatc ctgtcagtgt aataaagagt
     3061 tcgtaattgc gctgatcttt tatatagctg ttctcattat ctctctaccc tgaagtgact
     3121 ctctcacctg taaaaataat atctcaca
//
LOCUS       DAZSNM010000306         3112 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZSNM010000306 DAZSNM010000000
VERSION     DAZSNM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.309"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACHW42_004055"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACHW42_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR8945641.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="ACHW42_004056"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="ACHW42_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="ACHW42_004057"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="ACHW42_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFR8945642.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="ACHW42_004058"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="ACHW42_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFR8945643.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="ACHW42_004059"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="ACHW42_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          753 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAZSNM010000307         3097 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZSNM010000307 DAZSNM010000000
VERSION     DAZSNM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.310"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(104..1813)
                     /locus_tag="ACHW42_004060"
     CDS             complement(104..1813)
                     /locus_tag="ACHW42_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR8945644.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2245..2964)
                     /locus_tag="ACHW42_004061"
     CDS             complement(2245..2964)
                     /locus_tag="ACHW42_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HFR8945645.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          953 a          588 c          519 g         1037 t
ORIGIN      
        1 gccaattgag gtggttgcct gaaagttggc agttacccgg tcactacacc ttgttggagt
       61 gaacatatca aaaacaggta attacacaat ttaaattaca gtctcataaa aaatgattaa
      121 atctatttat tatttcaata aacgtttttt tcatcttctt tgaaaattgt tctgctatag
      181 tatccggttc cagattgttc tcttttatta ttctacaata ctctaatatt gtttctttgt
      241 ctctatttac tacagataca aatacaccag gaataccatc tttattagtt gcggacagca
      301 tttcgaccaa tcgtcttgtg gtaaaactat agttttttag aatattacaa aaggcacgta
      361 ttgtttcggt atggccatta taaaatgcca taaaaagtcc tgatgtaccg ttgggatttt
      421 ttatccataa taattcatct atatattcct ggtgaggggg gagtattttt agtatatctc
      481 catacgccat aatggccttt gtgtgcccat tttgaagtgc cataaacaac ccatgcttta
      541 ctttcaataa ttctttaatt tgttcactac taagcatttt cttattaagc agacttccga
      601 acatacgaat actatctgca tgtccattct gcaatgcaaa aaataaccca ggtaaatcat
      661 ggctgtcttt cgcagctaat agatataatc tcttatcgga agttaacggg gttttaaaga
      721 ctatttcccc ataggctttt attgtgtcta catgtccgtt cataaaagac atgcataaac
      781 ccggcacatt gtttgcccct accgcagcaa gcaatgtaag tattgtttct tgactaagat
      841 tgcacatatt taatatctta ccatatactt gaataatgtc tgaatatccg ttttgaagtg
      901 ctatataaaa tgcaggagta ccatcagagc ttctcgtggc cagaaggttc accagtttag
      961 attgagaaat ttcaccattt ttgtattttt ccatcagtat aaataatact tctgtcagat
     1021 tctttgttat cgcatgatat ataactctgg aattgatcaa ttcatctggt aaaagtttca
     1081 ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat tacctgcccg ggttgctcca
     1141 gaccataaat tttccaataa aaatcatcaa aaaaatcctt agcggataat tgactcagtt
     1201 cgaaatggct atcacaagta aatacagtac gtttatgagt aacagttcga ttaggatcga
     1261 aaaactgaac aacccatttt tcactttctt gtacgcgctt tattttcaac cctaacgcca
     1321 tttggtggtt taatgtgcat gctgataatg ttctgacaaa gtctccattt tttttcatgt
     1381 tataaaaaaa attagtaata actgttcccc actttttatt ttccacaaaa taaatataag
     1441 ctggtgattt tgaaaaatct tccgtttttt ccaaacatac attttttgca atagcatcag
     1501 gggtgctaag aagctgatag tttaccttcc catttgtttc cataaaattc atttcccaat
     1561 aggaagctag gtgacggcaa actatttgtc ttccatatat atcttttgcc actccattta
     1621 tatttacctc cacatttctc ttttctgaaa aataaggtgc tgttttattc ttaaactgaa
     1681 ttagttcact ggagtttgag accctcataa tagaacttgt tattgttcta ttatctctgt
     1741 taacagacat atctgttgat aacgaatttt ttgttgaaaa tatactagat gaaaatgttt
     1801 tatttaacgg catagaagac aaccatagag atgttataat actattagat tgcagttact
     1861 aatagtaaaa gcctccttac aaggaggtag cttattcaga atgctcctat aagatagcga
     1921 gctcgtcaac gatacagatg atctgattat attgtgcaaa aggagaacag gaaaataggc
     1981 actggaaaag atgaaggcct gatgggagca ttaactaatg caaaatccga caggttaata
     2041 tcccgaaaag caatcaaacg actcaaacag gagatgcacg ctgaaatggt ggacaactgt
     2101 tcggatttca gatgagtagc tcagcagttt atgagccgaa tgctctgtca cgggcaggaa
     2161 tcaggctgca tacgcagtgc caaatctaat cgtagttatt ctaaccagtt ttcaagctac
     2221 aaggtggtgt agctggcatc ttctctactc tatcatcaaa cgataaaatg gttcctctcg
     2281 taacatttgc tcttgccttt cacttccatc tcgatcgctt cgtagttcat tacgatagct
     2341 gacatatttc cagtcttcag gtctaacatc tgacgccgga tactccccgg gagcgatttt
     2401 gcttcttaat agattagact cgagacacat tataaattgt cgtattttat gaagtaataa
     2461 agcactatat tgcgagcatt cctgatctga ttttacatat agcgtaaact gagctcctat
     2521 ccccacacga gattgctgag aaactcgatt catatcagtt atcttccatt tatctatagg
     2581 actgtcctct gaaaataata aaccagacaa aatttggaat gccagaggaa cttgctccct
     2641 agctatacta atatgaaact tgtccccaac aaaatccccc ttactctgag gattttcacg
     2701 acatgcatga ataaacacat ctccactatg gttcacaatc tggaatcctt ggtacatgac
     2761 agaaaaagat tgacatatat cagagtaagt tggcacgttc gatgctactt tgaaatagta
     2821 cgctgggtat tgccgtattt ggttgtacaa tatattgaag ttatttttca atctttcatt
     2881 cgcagaaagc atctgcggta tttcagacct cacaacattc aaagaatcta aatttagttt
     2941 tagacagggc ttttttatgg gcatagaaaa cgtcctctat aaaatagata tattatctta
     3001 tattgattgt ttatcatctt ttataaaacg atacattata tgtaatgtat gtaagcgtaa
     3061 gggtcctctc cgaacatggt gggaaggctt agaacgc
//
LOCUS       DAZSNM010000308         3069 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZSNM010000308 DAZSNM010000000
VERSION     DAZSNM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.311"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..498
                     /locus_tag="ACHW42_004062"
     CDS             <1..498
                     /locus_tag="ACHW42_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8945646.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="ACHW42_004063"
     CDS             578..1273
                     /locus_tag="ACHW42_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR8945647.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="ACHW42_004064"
     CDS             1302..>3069
                     /locus_tag="ACHW42_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR8945648.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAZSNM010000309         3045 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZSNM010000309 DAZSNM010000000
VERSION     DAZSNM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.312"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1142)
                     /gene="gtrII"
                     /locus_tag="ACHW42_004065"
     CDS             complement(<1..1142)
                     /gene="gtrII"
                     /locus_tag="ACHW42_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HFR8945649.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQT"
     gene            complement(1139..2068)
                     /locus_tag="ACHW42_004066"
     CDS             complement(1139..2068)
                     /locus_tag="ACHW42_004066"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR8945650.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(2065..2427)
                     /locus_tag="ACHW42_004067"
     CDS             complement(2065..2427)
                     /locus_tag="ACHW42_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFR8945651.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            complement(2689..>3045)
                     /locus_tag="ACHW42_004068"
     CDS             complement(2689..>3045)
                     /locus_tag="ACHW42_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR8945652.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
BASE COUNT          968 a          593 c          597 g          887 t
ORIGIN      
        1 gtttgagatg tgtatgatat ttctttagct aaagagatat ctttctgtct tgcgagagta
       61 tcattcaaaa atagattttt ggatgttgaa acgccattaa atataataaa taaaaaaaac
      121 atgcagttta ggtattttat agagcgaaaa ttgcttaagc aagaaaacaa aattactgct
      181 actataggca ttaaaacaaa caggcgaggt ggcgcccctg agccaacaac aacgataaat
      241 ataaccggaa gtatataaat gaaaatgatt gatgaaatca aataaattga tcttgttttt
      301 aatttatatg ttagggtaaa catcagaatt aataaaggaa tcagtaacca cttgtatagg
      361 tttaaaccag tgggaggatt gttatagaag ttatatatat tatctatggc tgatttaaca
      421 agccatttaa tatcgcttgc attttgtata taatttgaga tgtactcgtt cgattcaagc
      481 gaaaaataat gcttgatagc tttggttaat agcaggtaaa ttaaggtaga taaagctatg
      541 agggatagag ataaacatga actataaaaa gattcacgaa tatttttctc atttcgtatt
      601 atcgaattta tctgcaaccc aatgacgaat gcaataatat atgttacgaa tgtttggtag
      661 attgccattg aaagaatgga cagtactata ccagaaaata tcacaatcct atttttttgc
      721 gagtgaaaaa taattgctga tatcgctgcc agtagaaaag caattcccac agtatcagct
      781 tggttaagaa actctaattg ataggagatc tgagggaagg taataaatac taacatacct
      841 atcaataatt cataagattc tagctttagc gatctgcaga ttataaatgc tgaaatgatg
      901 ataaaagata aggctattaa tggtgttata taaagtgaaa aaggctcagg gagaaagtaa
      961 tgtcgcaaaa aagtgtgaaa ccaccgccca agcgtgattg tttgataaaa attatttgta
     1021 aactcgccat caatattaag tgttccatcg tacaaccacc cccaataaca ataaattgat
     1081 attgcaaaac atgatatgaa ggcaaggaga tttgcatttt taaataaatt aattttaatc
     1141 atgggttacc tcgatgagat ttttttatga tgtatttggg tctatttttt acttcaatat
     1201 agattctacc tatatattct ccgagaacac caatcccgat cagttgcact ccacccaaga
     1261 aaagtattga tacaagcagg gagggatacc cgcgtactgg gttaccaaag acaagggtgt
     1321 ctataatcat ccatgcacca tataaaaatg aaatgcttgc aacaaacaag cctatataag
     1381 tccatacgcg aagaggaaag gttgaaaaac ttgtgatacc ttccagtgcc agattccata
     1441 atttccagcc attaaatttt gagatgccag caacacgctc tgcgcgtaca tattcaacga
     1501 catccgtcgg accacccacc cagctcagta tgcctttcat gaaaagattg cgctcaggca
     1561 acagtttaat gttctccaca acctcacgag acatgagtcg aaaatctccg acattttcct
     1621 cgatctttgg ggtgcttatt ttgttgtgta atttatagaa ccactcagct gtcttacgtt
     1681 tcagtcgtcc atcagttgag cggtctgaac gtttagcaag caccatgtca gcacctgcct
     1741 gccacttttc aataagatga gggataacct caattgggtc ttgcaggtcg acatcaatag
     1801 gaattacagc atcgccgctt gcatggtcta accctgcaaa taaggctggt tctttaccaa
     1861 agttgcgtgt aaatgacagc ggaacaacta gcgggtctga aacagccagc gcgttaataa
     1921 ttgactctgt ggcatcttta cttccgtcat ttatgaatac aatttctact tcatatggct
     1981 tcaactcttg gaattcacgt accgttttat agaaaacagg tatcgcttct tcttcattga
     2041 agacaggaac gacaagagat attttcattt cgcatcccta aagacaatga actttgaata
     2101 gacgaaaccg cacaccagac tgatggcgga gaaggtgata agagtgacta ttggaggaag
     2161 tgagcattta tcagctgccc aaccaacaat cacactcaat attcccataa atcccacgta
     2221 taacatgtag cgcatcgctg tagtcaatgc tttgaatgtg aatcttgcat tcgcgaagaa
     2281 gctaaagctc acagccacta cgaaacctgt gaagtttgcc agagcctgac tggtatgtgc
     2341 ggcatagata catacaccaa aaaccaccca gtgtataagt gtgttcagca cacctataga
     2401 tgtgtacttt acaaataact ttaacattta tttaatcaat gagctctgaa aggcatgaag
     2461 tctatcatcc aagtctcaat tgatcgatac ttgctatgtc tgatgagaca aaactgagac
     2521 acataaggcc tcacaatggc ttgcaaggct ttacatgttt tgatgtggtg ggacgtgtga
     2581 gcgcagtgtt gatgggataa tcctttgaat tacaagcgga ttcttataat tcgtaatgcg
     2641 aaggtcgtag gttcgactcc tattatcggc accatttaaa tcaataagtt acctcgcatt
     2701 taagtaaact acgttctcct cttgtgccgt atttgtgcca ttgcgactta taatcgcatc
     2761 gattttgctc gcgtgctcgg taagatgccc ggctgaaagg tgggcgtatc tttgaaccat
     2821 ttcgagagtt tcccatcctc ccatctcttt aagcgcaaga agagagacac cggactgaac
     2881 cagccagctt gcccaggtat gcctcaggtc atggaagcgg aagttgctaa tgcctgcccg
     2941 ctttaacgct cccttccatg ccttgttgct gtcggttctc atcttcctta ccgctgctgt
     3001 ttttgttccg tcgcttcggt aggcaggttt ggtgtggaca aacac
//
LOCUS       DAZSNM010000310         3044 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZSNM010000310 DAZSNM010000000
VERSION     DAZSNM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.313"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1283)
                     /locus_tag="ACHW42_004069"
     CDS             complement(<1..1283)
                     /locus_tag="ACHW42_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR8945653.1"
                     /translation="MANENLDIDSKTAEELYEWYLDDKLIINRRYQRKLVWSLEEKTA
                     LISSMTQQYPIPLLLFVSIDNKREILDGMQRLEALMSFIEQRFSLNGKYFNLDAIALT
                     KQLKDSGKLQQKEPVLSRESSTLIARYRFAISEYSSTEDHIDEVFRRINSNGKILSKQ
                     ELRSAGCVSNFSELVRKISTIIRGDTTHSDIMGLNKIHNISICNDGLDYGINIDNHFY
                     IRNHIISRPSIRDSDDEELVANILGYIFLDDKPTSGSTSLDTFYGEGSTSHAIHTRTQ
                     LENYIQTNGADKIVNNYLFVYEMIQKLFDANNLNFRSHILGNASSSQECPRYYQAVFL
                     ALYELIINENMQLDDEQKFIAQLGDSVQRSMVQTEGGRWAASARQKSVEDLCALIRRY
                     FKESENKFINHAWQTLIRTLLNNSRTEQPNYDFKQG"
     gene            complement(1568..1900)
                     /gene="pemK-mt"
                     /locus_tag="ACHW42_004070"
     CDS             complement(1568..1900)
                     /gene="pemK-mt"
                     /locus_tag="ACHW42_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HFR8945654.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
     gene            complement(1902..2159)
                     /gene="pemI"
                     /locus_tag="ACHW42_004071"
     CDS             complement(1902..2159)
                     /gene="pemI"
                     /locus_tag="ACHW42_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HFR8945655.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(2252..2905)
                     /locus_tag="ACHW42_004072"
     CDS             complement(2252..2905)
                     /locus_tag="ACHW42_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFR8945656.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
BASE COUNT          870 a          709 c          593 g          872 t
ORIGIN      
        1 ccctgtttaa aatcataatt tggttgttct gttcttgagt tattaagtag agttcttatc
       61 agtgtttgcc aagcatgatt gataaattta ttttctgatt ctttaaaata tcgtctgatt
      121 aaagcacata aatcttcgac acttttctga cgagcgctag ctgcccaacg gcccccttcg
      181 gtttgcacca ttgaacgttg tacactgtct cctaactgag cgataaattt ctgttcatcg
      241 tcaagttgca tgttttcatt tatgattaat tcatacaaag caagaaaaac agcttgatag
      301 taacgaggac attcttgaga tgaagatgca ttgcccagaa tgtgtgatct aaagttcaaa
      361 ttatttgcat caaataattt ttgaatcatc tcataaacga agaggtaatt attgacaatt
      421 ttatctgcac catttgtctg aatgtaattc tctaattgag ttcgtgtatg gattgcatga
      481 ctcgtcgaac cttcaccgta aaatgtatcg agcgatgttg atcctgatgt tggcttatca
      541 tctaaaaata tatagcccaa gatatttgca actaactctt catcgtcaga gtctctaatg
      601 ctaggcctac taatgatatg atttctaata tagaaatgat tgtctatatt tattccataa
      661 tcgagtccat cattgcaaat tgatatgtta tgtattttat tcagtcccat tatatcgcta
      721 tgtgtagtat ctcctcttat aattgtcgat attttcctta ctaactctga aaaattacta
      781 acgcaccccg cacttcgtag ttcttgtttt gataaaattt taccattgga atttatgcgt
      841 cgaaacactt cgtcaatgtg gtcctctgtt gaagagtatt cagatatagc aaatctatag
      901 cgagcaatta atgtagaact ttcgcgggat agcactggct ctttttgttg aagtttacca
      961 ctgtctttta gttgttttgt taatgcaatg gcatctaaat taaagtattt accatttaat
     1021 gaaaatcgct gctcaataaa actcattaaa gcttctagtc tttgcattcc atcaaggatt
     1081 tctcttttgt tgtcaatgga aacaaacaat agcaaaggga tagggtattg ctgagtcatt
     1141 gatgaaatca gtgctgtttt ctcttcgagg gaccatacta attttctttg gtatcttcga
     1201 tttataatta atttatcatc cagataccac tcgtataatt cttctgctgt cttggagtca
     1261 atatctaagt tttcattagc cattagatgt atctcgagta tttagttaag aaactgatag
     1321 gtcagtattt gcatcaatgt tcattgaatt tgtatccgaa gtcaatctga tgtaggaact
     1381 tcaaaactgc gcaaaacacg caaatacgca caagaaagta gggctgcgct tggaattcgg
     1441 aaaataaacc acatcaaaac tactttttag ccaaataacg tttgggaatc accagaatgg
     1501 tgggacaaca gcggttttag tgccctaaat cgtacgtttt catccagttg cccctcaaac
     1561 cccatgttca agtcagaata gtggacaggc ggccaagaac ttcgttcatg atagtctccg
     1621 gaacccgttc gagtcgtttt ccgccccgtg ctttcatatc aattgtccgg ggttgatcgc
     1681 aacgtacaac acctgtggta cgtatgccaa caccatccaa cgacaccgca aagccggcag
     1741 tgcgggcaaa attgcctccg ctggttacgg gcacaacaac aggcaggcgg gtcacgcgat
     1801 taaaggccgc cggtgtgaca atcagcaccg gccgcgttcc ctgctgctca tgacctgcgg
     1861 taggatcaag cgagacaagc cagatttccc ctctttccat gtcagatttc ctcctgacca
     1921 gtcgccggtg catccagcca ttctcgttct tcagctgata tttcagcatt cggatcacac
     1981 tgtgccagta gctcagccag tgaatattgc gggcgtctgt acggctcaac aatcagccgg
     2041 ccattatcaa tgaccatgcc aacttcatta tctgtgccca gagacagcgc attcagcagt
     2101 gccggtggga cggtcagcat aactgagccg ccaaccctct tcagtcgggt ggtatgcatt
     2161 cttcacctcc ataaaagtta tatttaaata taacatccac taaaaaaaca caccaggctt
     2221 taacgcacaa tgtttaataa aaatataact ttcaaccaaa cagtaaaccc agcgtggttg
     2281 cttccatatg cagcaatacc ggcagcagca ggccaccact tctgatcctg gccactgatg
     2341 taatcaaccc caccaggaac agttctgcca gtgtcagcag gttctgatac tggctgtgcg
     2401 cggcgacgaa caacaacgac gttatcagcg cccccagcca catcgtccag cagtaccgtg
     2461 aacggaagac gttcagcata atcccccgga acagcgtttc ctcattcaac ggggcaagga
     2521 taaagatggt cagcaacgtc aggatcacgt caggtatgga cttatcggca aaaagtttcg
     2581 tcataaatgg ctcagcaggc agagccagcg ccttaccgag cagaaataca ccgacataca
     2641 ccacggccat cgcaccgacc agccacggta cgccaacgtt gcgcagctga ccaacgaccg
     2701 gtagcggagc tatccaacgg cggtatacca ggaaaacaca cagcaggtac atcagaacag
     2761 taccatgact gaagaacaaa tagttttttc ctgatccata aagcagaacg gcctgctcca
     2821 tgacaaatct ggctccccaa ctaatgcccc aggcagccag cataaccagc ataaactgca
     2881 gatattgatt acgtgtttga atcattgcat cgcctgtaaa tttttaactt gtcctatttt
     2941 tgtcattacc acgtatatac acatgtataa caattcagat atcgttacca ggatatgccg
     3001 catcagcggc atggaaggcg gcactctgtt gtttcatatg atac
//
LOCUS       DAZSNM010000311         3017 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZSNM010000311 DAZSNM010000000
VERSION     DAZSNM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.314"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            2..2905
                     /locus_tag="ACHW42_004073"
     rRNA            2..2905
                     /locus_tag="ACHW42_004073"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          800 a          659 c          943 g          615 t
ORIGIN      
        1 aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga tgaaggacgt
       61 gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc gatttccgaa
      121 tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg ttaatgaggc
      181 gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc
      241 cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga
      301 agcgtctgga aaggcgtgcg atacagggtg acagccccgt acacaaaaat gcacatactg
      361 tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg ggaccatcct
      421 ccaaggctaa atactcctga ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa
      481 agaaccccgg cgaggggagt gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag
      541 cacgcttagg cgtgtgactg cgtacctttt gtataatggg tcagcgactt atattctgta
      601 gcaaggttaa ccgaataggg gagccgaagg gaaaccgagt cttaactggg cgttaagttg
      661 cagggtatag acccgaaacc cggtgatcta gccatgggca ggttgaaggt tgggtaacac
      721 taactggagg accgaaccga ctaatgttga aaaattagcg gatgacttgt ggctgggggt
      781 gaaaggccaa tcaaaccggg agatagctgg ttctccccga aagctattta ggtagcgcct
      841 cgtgaattca tctccggggg tagagcactg tttcggcaag ggggtcatcc cgacttacca
      901 acccgatgca aactgcgaat accggagaat gttatcacgg gagacacacg gcgggtgcta
      961 acgtccgtcg tgaagaggga aacaacccag accgccagct aaggtcccaa agtcatggtt
     1021 aagtgggaaa cgatgtggga aggcccagac agccaggatg ttggcttaga agcagccatc
     1081 atttaaagaa agcgtaatag ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg
     1141 ctaaaccatg caccgaagct gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt
     1201 ctgtaagcct gtgaaggtgt gctgtgaggt atgctggagg tatcagaagt gcgaatgctg
     1261 acataagtaa cgataaagcg ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc
     1321 caacgttaat cggggcaggg tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat
     1381 gggaaacagg ttaatattcc tgtacttggt gttactgcga aggggggacg gagaaggcta
     1441 tgttggccgg gcgacggttg tcccggttta agcgtgtagg ctggttttcc aggcaaatcc
     1501 ggaaaatcaa ggccgaggcg tgatgacgag gcactacggt gctgaagcaa caaatgccct
     1561 gcttccagga aaagcctcta agcatcaggt aacatcaaat cgtaccccaa accgacacag
     1621 gtggtcaggt agagaatacc aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa
     1681 tggtgccgta acttcgggag aaggcacgct gatacgtagg tgaagcgact tgctcgtgga
     1741 gctgaaatca gtcgaagata ccagctggct gcaactgttt attaaaaaca cagcactgtg
     1801 caaacacgaa agtggacgta tacggtgtga cgcctgcccg gtgccggaag gttaattgat
     1861 ggggttagcg caagcgaagc tcttgatcga agccccggta aacggcggcc gtaactataa
     1921 cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acctgcacga atggcgtaat
     1981 gatggccagg ctgtctccac ccgagactca gtgaaattga actcgctgtg aagatgcagt
     2041 gtacccgcgg caagacggaa agaccccgtg aacctttact atagcttgac actgaacatt
     2101 gagccttgat gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag
     2161 ccgaccttga aataccaccc tttaatgttt gatgttctaa cgtggacccg tgatccgggt
     2221 tgcggacagt gtctggtggg tagtttgact ggggcggtct cctcctaaag agtaacggag
     2281 gagcacgaag gttggctaat cctggtcgga catcaggagg ttagtgcaat ggcataagcc
     2341 agcttgactg cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg
     2401 gttctgaatg gaagggccat cgctcaacgg ataaaaggta ctccggggat aacaggctga
     2461 taccgcccaa gagttcatat cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat
     2521 cctggggctg aagtaggtcc caagggtatg gctgttcgcc atttaaagtg gtacgcgagc
     2581 tgggtttaga acgtcgtgag acagttcggt ccctatctgc cgtgggcgct ggagaactga
     2641 ggggggctgc tcctagtacg agaggaccgg agtggacgca tcactggtgt tcgggttgtc
     2701 atgccaatgg cactgcccgg tagctaaatg cggaagagat aagtgctgaa agcatctaag
     2761 cacgaaactt gccccgagat gagttctccc tgacccttta agggtcctga aggaacgttg
     2821 aagacgacga cgttgatagg ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta
     2881 ctaatgaacc gtgaggctta accttacaac gccgaagatg ttttggcgga ttgagagaag
     2941 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaattag
     3001 cctggcggcc gtagcgc
//
LOCUS       DAZSNM010000312         3012 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZSNM010000312 DAZSNM010000000
VERSION     DAZSNM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.315"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..613)
                     /locus_tag="ACHW42_004074"
     CDS             complement(<1..613)
                     /locus_tag="ACHW42_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR8945657.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRVMLPT"
     gene            complement(680..1405)
                     /gene="fimC"
                     /locus_tag="ACHW42_004075"
     CDS             complement(680..1405)
                     /gene="fimC"
                     /locus_tag="ACHW42_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR8945658.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1442..1981)
                     /locus_tag="ACHW42_004076"
     CDS             complement(1442..1981)
                     /locus_tag="ACHW42_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8945659.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2046..2594)
                     /gene="fimA"
                     /locus_tag="ACHW42_004077"
     CDS             complement(2046..2594)
                     /gene="fimA"
                     /locus_tag="ACHW42_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR8945660.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT          795 a          727 c          656 g          834 t
ORIGIN      
        1 aagttggcag cattacccga ttctgtacac tatttccgct gaaattataa ttgagcaatc
       61 cggcattaat accgggatcc cataactcag gaggaatata accacgcgcg cgattactca
      121 taaatgcctg agggatcgtc aggttcagtc gctgctgacc aacatccaga tgcgcagtag
      181 cgtcctggac cattgtggtt aatggcacac aggcatcatc cgccagcaga ttcataccgg
      241 cgacagaagc cgtattcagc cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa
      301 tcccttgttc actgtcgccc gtattaaatg tgacatcacg cgttgccata taaccattat
      361 tcaaatagat atcgacgcga tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg
      421 ataaatcggc cacagcctgg ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg
      481 cagatgacaa aggtgcctgt gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac
      541 cagccaaacg atgcttacga atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat
      601 tcagatatga cattattcct ccggtttaaa atcacccggc agtcaattct ttttattcag
      661 gcgaaaaatt ccccctgcgt tattccatta cgcccgtcat tttgggggta agtgcgccat
      721 aatcatttat tgttcggtaa gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc
      781 tttcgcccat tggaggcacc aatgcatttt caagaacccg ggttccggca ttcaactctg
      841 ttaccgtcag gtaatagggt gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa
      901 atcttaattt ttctgcggcc tgatcgggtg gcaacgctaa tttagccggg cgatagtaca
      961 gtttaatgcg gctgataatt gcgagctgta gcatattctc agtcaatttt gatttatcca
     1021 ttgacggaat cgctttaacg ttcatccaga ataaactttc ccggtcctgt ggcaattggt
     1081 tatttgttgc atcaagaata cgtaaggtat tctctttttt tcccttcatc gcaaacagag
     1141 gaggcgtcac gataaaacga ccatccttta caccatcggc attttccacc catgattgaa
     1201 ttaaataggt actattttca tcattatttg tcacggcaag ttgcacttgt ttttgccctg
     1261 ccggataaat tacgcgagtc gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca
     1321 tcattgccat gaacatcagg atacctgcca gcaagcagaa tgttatttcc tgcgatttcc
     1381 ttacattgac gtttttatta ctcactgtcc tgttcctgtt atcacattat ctactgaaca
     1441 attactgata ggttaaagag aaccaggcct gggcattggc gatgccgcca gtaacccgac
     1501 gcccggtagc acgatatttg gcgatgaaat gtagcgaagt agagcctgag taaagccgtt
     1561 tccagtttgc tggaggacga ttaatcggta cgaggtttcc ttcatcatca aacaacgcta
     1621 caccaatatt ggaggctatc cctggcccct ctcccacgga gagcacatcc ggatttttac
     1681 catccgcgac accgtgaaac gccacaccca cacgttcact caccaccgtg ctacattccc
     1741 gtaaatgaat aacaaaaggc accggtgcgc tatcttcccc tgccgcatga aaccggttac
     1801 tgctgatttg ccccatattg accgtcattt gtttatcacc ggcttcaatc cggcaagttt
     1861 ccgcaataat gacgccctga aattgcatat ttccgccggg cagcgtggta ttccatttat
     1921 ttccggccag agcaaacatt ggcagcaacg acgctaacag aaataatctc ttccttttca
     1981 tcgttatttt tatcgcacaa gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg
     2041 gtaggttatt gatactgaac cttgaaggtt gcatccgcat tagctgcacc cggggttgcc
     2101 tcgccgattg cataataacg cgcctggaac ggaatggtgt tggtaccgtt attcagggtt
     2161 gtttgtgcac taaatgtcgc accgtccagc gtcagagcat tgcctgttct gtctaggatc
     2221 tgcacgccaa cgtttgttgc actacctgca gcagaactct gcagagccag tacatcagta
     2281 cgcgtagcat caattgccgt acctaagaag gcaacagcgg cttttgtggc aacagtggta
     2341 tcgcaatcat tcagctgaat gttaaaacca acggcagagc tggttgctcc agcctgcttc
     2401 aggctagcgg tacgaacctg tcccaactga acggtttgat caacagagcc tgcatcaact
     2461 gcgcaagcgg cgttaacaac ttccccttta aagtgaatgg tcccaccatt taccgtcgta
     2521 gtatcggcca gagccgctgc ggaactgagg gacagagccg acagaacaac gattgccaga
     2581 gttttaattt tcatgctgct ttcctttaaa aaaactattt ctaaatcgac atgggcagtc
     2641 gttctgtaca ctttgttttg tcaacgagtt tgactgccaa cactgcacag ttttccccca
     2701 aaagatgaaa catttggggc cattttgact catagaggaa agcatcgcgc acaaactttt
     2761 tcagtttatt tgttggctta atattctatt attatcttta tttatagatt tttatattgc
     2821 atgaggtggt ttttggagag aagaatgagg aagatgcgtc gagccacaga aacgttagct
     2881 ttacatatag cggaggtgat gtgaaattaa tttacaagag aaataattta catatcaaat
     2941 agttagatgc tttttgtcgt tttttaatat ttttatgctt gagaaaaaat acgtaactta
     3001 tttatgatat gg
//
LOCUS       DAZSNM010000313         3003 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZSNM010000313 DAZSNM010000000
VERSION     DAZSNM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.316"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..195
                     /locus_tag="ACHW42_004078"
     CDS             <1..195
                     /locus_tag="ACHW42_004078"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945661.1"
                     /translation="VTVKRLRETIFFKQRRYGETGDFTVKYCSKKTLIKAKFLLKQPI
                     KNWRVAGEKLEFSQRIQIIF"
     gene            complement(169..1149)
                     /locus_tag="ACHW42_004079"
     CDS             complement(169..1149)
                     /locus_tag="ACHW42_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR8945662.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(1149..2348)
                     /locus_tag="ACHW42_004080"
     CDS             complement(1149..2348)
                     /locus_tag="ACHW42_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR8945663.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(2522..2656)
                     /locus_tag="ACHW42_004081"
                     /pseudo
     CDS             complement(2522..2656)
                     /locus_tag="ACHW42_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2670..>3003)
                     /locus_tag="ACHW42_004082"
     CDS             complement(2670..>3003)
                     /locus_tag="ACHW42_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8945664.1"
                     /translation="WLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTSD
                     DWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIGS
                     FIEKHMFY"
BASE COUNT          854 a          704 c          618 g          827 t
ORIGIN      
        1 gttactgtta aacggctaag agaaactatc tttttcaagc aaagaagata cggtgaaaca
       61 ggcgatttca ccgtgaaata ctgttcaaaa aaaacgctaa taaaagcaaa atttctttta
      121 aaacagccaa ttaaaaactg gcgagttgct ggcgaaaaac ttgaattttc acagcgaatt
      181 cagattatct tttagtacaa gagcaatcat acgatctaac ttatcctgaa cttccaacgg
      241 taatctgccg aattcatatg agaacgtacg tccctttact cgttttcgag caaatttatc
      301 tttactatca aatttccata acaaagttac aaccgcttta tcctttactc ctgaatcaag
      361 caatgaagac atttccttgg ttataagccg cataatactg tttttaacct cgtcttcaga
      421 aagattgtca tcagcagtaa taagctcaat actcggcgag atattcttca caacttcatc
      481 aatagatata tcacccagcc tcagtcgttc acttaattca gccagttgcc tgtaatcggc
      541 gtatgtcagc tcagactgaa caggaaaaag agacacaaaa tctttaggta cggacgctgc
      601 ttggagggca cgagtcacct tagctgccga caatccctct ctttccgcaa tttgcttctg
      661 gttcataccg gcctctttga gacgtactaa acgcaaccca atttcacgaa tattatgttc
      721 aagtgacgtc tgcaaatctt tcgccagatg ctgagcttca gaaacagtaa gctcatcatc
      781 cgttacaagt acacgtaatc caaccttgca tagcaaagct gctgcccgac gacgggaacc
      841 atccaaaatc tcaataagat cgccagtccg aatcccaata catggataga attgttgtaa
      901 acgtatagtt ctggtgatgt cttttagtga gtccggcgtc agggctgtct gttcgcggcc
      961 attcacctct tcaaccacaa aagtttgagt atccactttg tcatgatcaa ccttgatctc
     1021 agtaaatttt gcctttcggc cagtattgag ggtaaataca tgaactggag ccgatatttc
     1081 ttcagtatta ttcaggatat ttgtattcag cgtacggcca atagtcggtc ggtgctttct
     1141 gttttccatc agtagttggc ccttataaat tcaatacgat caaaaactgc tttagcgaaa
     1201 tcttctgctg ccatacgtgc atttttcaaa gcttcaccac tgccaacata agtaacagga
     1261 ttcgcagata tcacggtatc aaacgactcg ccgcttctct caaaaccatc aagacgtggc
     1321 attgaaacat caagcatgtc accaccgaaa atctcttttg tcaggctatg gcagtattta
     1381 tgatctgact tattcgccag tttcgacatg aagccaatat ttgcctgcaa acggcatgaa
     1441 catccatcct gttcaataat ctgtacaagt tcaggtaaac gagccaaata tttaagcgtg
     1501 gaatggaaat caacttgtgc tggcggcacc ggtgtaaaca tgatatctgc ggcagcaatg
     1561 gcatttttca aaaatgcgtc cagatgcggg cctgtatcaa taaggatgaa gtcgaaatca
     1621 tgcttcagct tatcaataat attctcacgc aatatcgcgt gtttgttttg tcctaataga
     1681 tgctcctcgc acagagtatc ccagtttgat gcaataaagg cgtcgtcaat agaagccggc
     1741 ataacataaa cgccagggat aactgacgga acgataaaat cttccagtaa ttcctcacga
     1801 ctcacgttct gcaacatagc ctgcggtgcg gttgtatcaa ccagccccac cgcatgcaag
     1861 tagttcagaa acatagtggc tgatgactgg ggatctaaat caaggagcag aatacgtaaa
     1921 tcttctgaca gcaggtgagg atgagcacgc agggcgtgag ctacggaaac actactcacg
     1981 gttttagaca caccgccttt tagactaccg atgaatatgg aatatgcctc actataacga
     2041 tcgcgatatt taggaatgcc acgatgtgca tagatatcga taatgttctg gatggttaat
     2101 gcatattttg tcgcggttcc tgctgggcgt ttttcaaact gatagccctg agcctccatc
     2161 tcaccgactg caccatccac gcttgcgcgc gttaattttg gaagcttagc tacagcagac
     2221 tttgaataga cttgataaaa aacatcagcc tgaaactcct gcttttgctc ctgaatctgt
     2281 tttgttaggg cgagcaacat tttatctgca cgctgcgcaa ccttacttaa ctgctcaaaa
     2341 ctagtcattc cccctccgtg ctatgtagga ttttaactta ttacaaaaaa aactgcatta
     2401 gcagggtaat gcatgcaagt acatattgta catgcttata ttaaaaatgc gcgcattgct
     2461 gatttattaa tcatcggaat gaatattact gtaagatagt atgcctttcc gggagaaaga
     2521 cgctgatttc ccccgaataa tccagttcac caccacactc cggacaacag ctttcttcag
     2581 gctccagatg gtgtatttcg cgggaaacat gttccggtaa cggacggcga tgacgtgact
     2641 gtcgcaacag gcgggggtag tgcttccaat cagtagaaca tgtgcttttc aatgaaggag
     2701 ccgatgactt tttcgtggat ttccacggaa cgcgagaagc agattgtttt acgtgccagc
     2761 cgcttgatgc gggtgcggag ggtccggttg ttacgtgcaa tacgttgcgt gaagattttg
     2821 ccggtcaggt gtttctcctt cggcacttct ctggcgtagc tgccccagtc atcgctggtc
     2881 accatgccga tacagaacgg agtcaacagg gctaacagtt cccgacaggt ctcatcattc
     2941 cgtggcccga aagtgtaagc caacacaccg ccagttttag tgttgtacgc ataccagagc
     3001 cag
//
LOCUS       DAZSNM010000314         2982 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZSNM010000314 DAZSNM010000000
VERSION     DAZSNM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.317"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            60..983
                     /locus_tag="ACHW42_004083"
     CDS             60..983
                     /locus_tag="ACHW42_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR8945665.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(1021..2661)
                     /gene="trg"
                     /locus_tag="ACHW42_004084"
     CDS             complement(1021..2661)
                     /gene="trg"
                     /locus_tag="ACHW42_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR8945666.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
BASE COUNT          718 a          775 c          701 g          788 t
ORIGIN      
        1 gttatcataa tcaaacaact tcacttgtca gcgacaccgc ttcgttttta acatcgctta
       61 tggaaaaaaa tagtctgttt agtcagcgca tccgtttgcg ccaccttcat acattcgtag
      121 ctgtcgcaca acaaggaact ttggggcgcg cagctgaaac ccttaatttg agtcaacctg
      181 cgctctctaa tacattgaat gaactggagc agctgacggg cgctcgcttg tttgagcgtg
      241 gtcgtcaggg ggcgcaactt accttacccg gcgaacaatt tttaacgcat gcagtcagag
      301 ttcttgacgc catcaacact gccggacagt cgcttcatcg taaagaaggt cttaataatg
      361 atgtcgtcag ggttggtgca ctacctactg cggcactggg gatattacct tcggttatag
      421 gtcagtttca tcagcaacaa aaagagacga ccttgcaagt tgcgacaatg agtaacccta
      481 tgattctggc gggtttaaaa accggggaaa tcgatatcgg cattggtcgg atgtcagatc
      541 ctgaactgat gaccgggctt aattacgaac tgctgtttct tgaatcgttg aagctggttg
      601 tccgccctaa tcacccgcta cttcaggaga acgtaacgct aagccgggtg ctggaatggc
      661 cggtcgttgt atcaccagaa ggcactgcgc cacgccagca ttcagatgca ttagtgcaga
      721 gccagggctg taaaattcct tcgggttgta tcgaaacgct gtctacttcg ctatctcgtc
      781 aacttaccgt tgaatacgat tacgtgtggt ttgtcccttc tggcgctgta aaagacgacc
      841 tgcgtcatgc cacgctggtg gccctgcctg ttccgggaca tggtgcaggc gaaccgattg
      901 gaatactgac ccgcgtagat gcgacgttct cttctggttg ccagttgatg attaacgcta
      961 ttcgaaaatc aatgccgttc tgaaaggtga agggatctgt cgatccctcc ttgaacattt
     1021 tcacaccgta gcgaaactaa ctggttcacc cgctccgcga ggttctgccg acacagaatg
     1081 tttgttcaga cggaatacgt tcaccgcctc agttaatcgt gcagcctgtt cttcaaggga
     1141 caccgccgct gctgaggcct cttctaccag agaagcattc tgttgcgtca ccttatccat
     1201 ttcagaaatc gcctggctaa cctgagttat gcctctactt tgttcatccg aggcggcggc
     1261 gatttcctgc atgatatgtg tgacactcgc gacggcatca acaatagtgc tcatggtttt
     1321 ccctgccgtt gccacctcat ccgaccccag gtcaattaac ctgactgatt cactgatcaa
     1381 gccttcaatc tctttcgctg cctgagcgct gcggcttgcg agtgtccgta cttcgctggc
     1441 gacaacggca aatccacgcc cttgctcacc cgctcgcgcg gcttcaacgg cagcattcag
     1501 tgccagaata ttcgtctgga aagcaatact gttgatgacg gcggtgatct cagaaatttt
     1561 cttcgaactc gtggagatag cgcccatcgt ttttactaca ccggaaaccg tctgcccgcc
     1621 atcgctggct ttaatagaag cctcttgcgc cagtttgctg gcatgatgcg cgttatccgc
     1681 attctgtttc accgtcgcag tgagttgctc catgctggcg gcagtttgtt cgatagccgc
     1741 cgcttgttct tcggtgcgag atgacaggtc cgcattgcca gctgaaattt cgctggtgcc
     1801 acgataaatc tcttccgcgc cctgtcgaac agtccctact gtcatcccca gtgaatgctg
     1861 catttgctgt aaatgacgac ttaagcgacc gatttcatta cgacccgccg gttcatcatt
     1921 catcgtcaga tcgccactgg cgattttttc aatccgttgt gcggcatgtt gcagtggacg
     1981 aatgacgatc cgacgtagca ccataaatgt tatcagcgtc atgaccaggg caagcacaaa
     2041 cgcgccaatc atgaacatcc cacccaggcg ggtgcgctga tgggccagtt ccgccagttg
     2101 gttggctctg gtgctacgta tcttaacggc tttgttcaaa atatcggtat aagcattatc
     2161 cagcggtcgg atctgctcac tttcatgatt gataatcgct tcaaacatgc catttttggc
     2221 atatttcaac ataggttgca tacccgtgat ataagcctga aagcgttgat ttaattcagt
     2281 gtcgaaggct tcatcagcag gtgttttcac cggtcgattc tgataagcac gataaccttg
     2341 ctgcgactgt ttaatctccg attcggcttg cgcaatattt cgcttcattg cttccatttc
     2401 cgcaatacga ctcgcagccc cggcctgaat catattgatc cgggctgaac gcaaaaaatc
     2461 tgaactgttc gctaacccgg tccgaatctc aatctccgca gtaacatcac gctgatcgcg
     2521 atcggcctgc cagaggaaat agccagccag agcagaactt aatgcgaata gaactaagat
     2581 accgccaaga atgcaggcaa ataacggaac cagcctgatg tgatgcaaaa aacctaatcg
     2641 ctgtgaggga gttgtattca ttaccgttgt ctctcgtcca ggtttactcc tgatagaaag
     2701 tcatcggcac ttgacggtaa ttacttatga ccaaaaacct ctttgcgtca catttttaca
     2761 acaattattg cagagaaaat taatgctatt ggaatgaaga gagtaacgaa tggctaaact
     2821 ggctaacccg aatgacgaaa aattctggaa aacagcacgc aatttcgtga agtgattatt
     2881 ttcgtcccta aggaaacctc ataggatgcc tgcgggtgcc tgagactttc tgttttcagg
     2941 ctaaccggga atcagaaaga cgaaggcccc gaccgatatt tc
//
LOCUS       DAZSNM010000315         2976 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZSNM010000315 DAZSNM010000000
VERSION     DAZSNM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.318"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="ACHW42_004085"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="ACHW42_004085"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFR8945667.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="ACHW42_004086"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="ACHW42_004086"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFR8945668.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1133..1891
                     /gene="tam"
                     /locus_tag="ACHW42_004087"
     CDS             1133..1891
                     /gene="tam"
                     /locus_tag="ACHW42_004087"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFR8945669.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1895..2809)
                     /locus_tag="ACHW42_004088"
     CDS             complement(1895..2809)
                     /locus_tag="ACHW42_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFR8945670.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEEPEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          778 a          694 c          794 g          710 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aaatgtctga
     1141 ctggaacccc tctttatatc tgcacttttc cgctgaacga tcgcgtccgg cggtggagct
     1201 gcttgccaga gtgccgctgg agaatgtcga atatgttgcc gatcttggct gtggcccagg
     1261 taacagcacc gccctgctgc atcaacgttg gcctgcggcc aggataacag gcatcgactc
     1321 gtctccggcg atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc
     1381 agacatccgc aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact
     1441 gcaatggctg cccgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca
     1501 gggcgtgctg gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg
     1561 cgaagttgct tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca
     1621 tgcttactac gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccactta
     1681 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
     1741 tccgtggtta caggatctga ccgagagcga acagcagctt tttcttacgc gctaccatca
     1801 gatgctggaa gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg
     1861 tctgtttatt gttgcccgcc gtacggagta attatcacgt cagctggtaa tgacgatcgg
     1921 gaagaatttt tgctggaatt tctgcttcat cgttcatctg taacaggtca atttcaatag
     1981 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaacgga tcttccggtt
     2041 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
     2101 tcatgtaatg caggtccacg accagcgcga acggcagcat gatacaaaac agataaacgg
     2161 tacgatgcaa aatcagggtg taggcaaagg gaattggcgt gtaggcaatg cgctcacagc
     2221 ctgccagaac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg
     2281 aaagctgtcc attgcggcgc tgaaccgcca accattctcc cattattaac aagatacggt
     2341 tagctggaga gttcgaagcc agtacacgct gaagatctcc agtcttgaga taatgagcca
     2401 gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt
     2461 gcagcctggc aaactccctt acacttgccg aatccggcaa tgtcgttttt acctctcgca
     2521 gtaacgaccg tgaggcaatc atcaactgcc cccaaagttt tcgcgcttca acgtaacggg
     2581 cgtacccggc attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga
     2641 acggtgcgag ggtgaatttg atgcccagat gcgtgtacca gggcagcatg aatataacag
     2701 caatagaaaa gagaaaattg agtagtaagc gcgaggatat cttggataat actgagccgt
     2761 gccagacaaa aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca
     2821 ggcgtggaaa agtcgcccta ttaaacgtga ttacgatcac attctcaaga cgctacttac
     2881 aaattaccta gaaccacggc agcctgacgg ctgcaaattg aaaactgccc ctgttcgtcc
     2941 tggtgttgcc gcacgctccg cagaatatgt atgctc
//
LOCUS       DAZSNM010000316         2951 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZSNM010000316 DAZSNM010000000
VERSION     DAZSNM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.319"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="ACHW42_004089"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="ACHW42_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR8945671.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHW42_004090"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHW42_004090"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR8945672.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="ACHW42_004091"
     CDS             2603..>2951
                     /locus_tag="ACHW42_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR8945673.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAZSNM010000317         2932 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZSNM010000317 DAZSNM010000000
VERSION     DAZSNM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.320"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="ACHW42_004092"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="ACHW42_004092"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR8945674.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="ACHW42_004093"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="ACHW42_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR8945675.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHW42_004094"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHW42_004094"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFR8945676.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2756..>2932
                     /locus_tag="ACHW42_004095"
     CDS             2756..>2932
                     /locus_tag="ACHW42_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945677.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELW"
BASE COUNT          659 a          800 c          806 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt gg
//
LOCUS       DAZSNM010000318         2894 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZSNM010000318 DAZSNM010000000
VERSION     DAZSNM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.321"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..308)
                     /locus_tag="ACHW42_004096"
     CDS             complement(<1..308)
                     /locus_tag="ACHW42_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR8945678.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     "
     gene            complement(355..1074)
                     /gene="btsR"
                     /locus_tag="ACHW42_004097"
                     /gene_synonym="yehT"
     CDS             complement(355..1074)
                     /gene="btsR"
                     /locus_tag="ACHW42_004097"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR8945679.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="ACHW42_004098"
     CDS             complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="ACHW42_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR8945680.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          748 a          774 c          657 g          715 t
ORIGIN      
        1 tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt gttattaatg
       61 cgacgttccg gctccagtgc cggagcagac tcatccttgc cgcgtttttc ataaatcagg
      121 ccattgagga atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct
      181 ttacgtagcc agacggcgat ctgttccgcg gtggcttcga cattatccag cgccagaata
      241 cgcaccaggt cattattatt ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta
      301 cttatcatga ggccttcagg tgttgatgag gcaaaaagcc attttagcag tcttttacag
      361 gccaatcgcc tcttttaagc ttttcagata acggcggctg accggcacgg ttaagccatt
      421 acgcaaaatc aactcggcct ggccgttatc ttccagacga atctcctgta aatgcgcgag
      481 gttaaccaga tactgacgat ggcagcgcag tagtggtgta cgactttcca aggtacgtaa
      541 tgtcaattcg gtaaagccct ctttcccttc gtggctggta acgtagacac cgctcatccg
      601 actgctgaca aatgccacat ctttcatttg cagcaaataa atccgactat gccccgtaca
      661 agggataaat ttcagcgcct gttgattttc cggtaacagc gaaacatcct gcttgctgcg
      721 ctcctgacgc aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg gcttcagcag
      781 ataatcaaag gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa
      841 aacaatatac gggcgatgtt ccgggtcaag catccccacc atttccagac cactgatgcg
      901 cggcatctgg atatcgagaa acagcacatc cgggcgcagt ttatgcaccg cgccgatccc
      961 ttccacggcg tttgaacact ctccaacgat ttcaatatcg ctctgctcct gcaaaaatat
     1021 acgcaggttc tcccgtgcta acggttcatc atcgacaatt aagactttaa tcatgcctcg
     1081 tccctccatg gtagtcgtaa cgttattcgg gtgtaactat caggctcaca ggcgacgctt
     1141 attccatagt catcgccaaa ccgttcacgt aaacgcttat ccaccagatt catccccagc
     1201 ccactggcat tggttaccgg ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa
     1261 tgttgcccct cacgtcgggc gctgattgcc actcgccctg tatccagcag ttgtgatgtc
     1321 ccatgtttaa tggcgttttc cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg
     1381 gataattctt gcggaatagc aatgttgacc tgcaaccgcg actggaagcg cgccttttca
     1441 atttgcagat aagcgttcac atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc
     1501 cgctttaagt ttttgcggaa aaaagtggaa agatactgca ccagctggct ggcctgttcg
     1561 ctgtcgcggc ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa atgaggattc
     1621 acctgggcgt gaagcagttt gatttctgac tgggtgagca tcgctttttg ccgctcatat
     1681 tgccctgcaa gaatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt
     1741 attgaactga ataaccggtt tttggcttca tacaatttga tggtgcccat cacgcgctga
     1801 ttttcgccac gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag
     1861 caacgataag gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt
     1921 aaggtgtaag tcgaagaaat cggtttgccg ggtaaatggt ggtcgtcacc aattccggta
     1981 aaggccagca atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac
     2041 agtacctgag ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc
     2101 gtcgaggctg ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc
     2161 gcgcgtttat cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc
     2221 attggcgcag caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgca
     2281 aggatgatca gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg
     2341 ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc
     2401 aatccttcaa cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat
     2461 cgatgtaagc cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg
     2521 cccattaccg cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac
     2581 caggtgccca tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga
     2641 cgaaccgtga cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg
     2701 actaaaaaaa cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc
     2761 gcaaaccaca cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga
     2821 actcttgcag aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata
     2881 aaatcaccct atag
//
LOCUS       DAZSNM010000319         2875 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZSNM010000319 DAZSNM010000000
VERSION     DAZSNM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.322"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            210..971
                     /locus_tag="ACHW42_004099"
                     /pseudo
     CDS             210..971
                     /locus_tag="ACHW42_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(1078..1590)
                     /locus_tag="ACHW42_004100"
     CDS             complement(1078..1590)
                     /locus_tag="ACHW42_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945681.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(1729..>2875)
                     /locus_tag="ACHW42_004101"
     CDS             complement(1729..>2875)
                     /locus_tag="ACHW42_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR8945682.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT          857 a          644 c          524 g          850 t
ORIGIN      
        1 gcctattcct ggtggaactg acacaagaaa ctaaaataca aatataaccc tgaaaaagtc
       61 atataaaact tatatccaca gaataaaaaa tcaaacatgt cagttttatc aatataaagt
      121 atcgcaaggc gaaaagtgat gcaagaatac tcctaagtaa atgaaagcgc accacatgaa
      181 attatcaaca catgtaattt catcattagg tgctatcgaa aataactcag gagctactta
      241 agatgaataa tgtcttactc atgagacccg atgactcaga atttatgata cctgatgact
      301 ggtcagggct aaacgcaaaa ggcattacat acgaacaaga taatagagtg acagagtctt
      361 ttctgccttt taaatctttt tattgttcac cggtgtgcac gacagttgaa ctcatctcat
      421 aaaataattg ttttgctatg ggtatccgct accttctgga aaagaaaaga gacttaaggc
      481 taaatcactt taaagccttc tctcataaat gcgagacaaa acctgacgtg ttaattatga
      541 ccgggtgcac cgtcgctgat acttatagta tattctcacg cattgttgaa gaaaatataa
      601 tgaaaacgat gacacctatc gtcatcatta ctgggcatca ctcagaagat ctttgttctc
      661 tggcatcgaa actgggagga gttaaaataa tatcaccgac agaaaagatt gcaaaaatag
      721 aaaacgctat caatgatgct tttaacagca agcctaaaaa aataagattt ttaacagata
      781 tactctctcc tcaacaaatt gagaccatga aaatggttgc gcagggtaga tccgttaatg
      841 agatttcagc cgcaatgaat attagcatca agaccgtgta tgcacataaa gttcgtgcta
      901 tcaataagct gggaattaag aaaaaagttt atgaagcgct tttttataaa gcgctaattc
      961 agttcaactg atgtaaaaat caccctcgct taataaataa tatattctat gtcttttttt
     1021 ataagttaaa aaaggcggga tatcaaatcc cgccatgaaa ttaactaatc atctgcatta
     1081 ttctgcgtag atataagcct gggtacggat ggtgtaagag tctggctcaa caacctgttc
     1141 tttcatatgg gaaacgacgc gataggcacc acctgttcca gtatttttca gcatcatata
     1201 acgcttaccg ccaatctctt cggaggcagc tgaaggagga tcctgctcaa gcgttgtcag
     1261 ggaaactttc agtttatggt ccgggttatt gtcaccagaa attactgcat ccgctgaagt
     1321 actgctctcc gcataaatat catccgggaa agttacagca tagtcgtggc tctgcccatc
     1381 agatgcgcca acaatacctg ttgccaaagg cgtccatgca gttaacgtac cgctatatac
     1441 ccctttcact ggttgaatgg tattattaac aacgataggc ttggtcaaag taagattagc
     1501 tgacgcttca gtaccctggc ttactaaagt tcccgcctga gaagcaaaag ctgtccccgc
     1561 cagcatgatt gctaacaatg ttttcttcat tatattttct ccatgatttt catttacact
     1621 gcaaaatgca gtttctttct ttgcatttac gtgctatcgc gaaaatgctt ccaccaaata
     1681 atggaaacat cttcacaaaa ataaattatg agtttcttaa tttagaaact ataaacatgt
     1741 cgccgctatc tggtataacc ctgatgcact tttcttcgca ggaagttggt attttgcgat
     1801 acaggattga tctttagaat aaccccattt cacattaaac tcgccttccg cactcaagcc
     1861 agaaacataa gcaataccat cttcacctac aatgccggcc atattttcag tgtctttgct
     1921 ataactaatc acagcgccga aggggatggg tttaccctct ttctgtttta aggttactaa
     1981 tcctttatcc ccgctcttca ctgccaatgt cgcaggcaca acggctccag ctgtcggtac
     2041 aaccaattga ctagtttgtg gtaagtcgta attcttacct atcgtttttt catccagaat
     2101 aacatcgtct cttcggtatg gctgcactgg cagtattgca tacccacgaa aatcagtact
     2161 cagtcccggc cagccattaa cgttaacccc cgttgctccg ggtgcctcta taagggctaa
     2221 tgtcccatcc acattttgac caaatgtaag tccatccctt gtaataagta tcccaccgct
     2281 taaatttgca cctagctgtt tcatgttttc gctggagctg taatggctac taaactgacc
     2341 atatgatcca tttttgccaa cttgcacaat gttcatcgtg ctcttcggtt gtgactgatg
     2401 agcgttagtt atatttgtcg ctacatccca ccatacacca ccactattct gacctgataa
     2461 tgaaacctga tagttattac cctgctctcc cgaacgactc atctgtgaag acgcataaat
     2521 ttgattttcg tcacccagcg ataatggcac tgaaataccc acgcttaatt tccgatctac
     2581 tttccattta tgcgatgtca gcaagtcgtt ttttgcccag ttaatggaga atgttatgcc
     2641 tctccatacc gtactataac tcaggctggt agaccgcgtg tttcctgtgc cccgggaaga
     2701 attatacctc gtggtattga agttcaaatt cccgatatta ccaaggggct gggataagga
     2761 tatcgtagag ccatcgtgac tattcttatc tctttttctg ttccgttcat cctgtgcact
     2821 tactgcttca gtcagtgtgg cataacctga tgcataacgg ttcgtggtta tagac
//
LOCUS       DAZSNM010000320         2854 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZSNM010000320 DAZSNM010000000
VERSION     DAZSNM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.323"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..2226
                     /gene="ptsP"
                     /locus_tag="ACHW42_004102"
     CDS             <1..2226
                     /gene="ptsP"
                     /locus_tag="ACHW42_004102"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR8945683.1"
                     /translation="DSVQPTQAELTAHPLPRSLSRLNPDLLYGNVLASGVGVGTLTLL
                     QSDSLDSYRAIPASAQDSTRLEHSLATLAEQLNQQLRERDGESKTILSAHLSLIQDDE
                     FAGNIRRLMTEQHQGLGAAIISNMEQVCAKLSASASDYLRERVSDIRDISEQLLHITW
                     PELKPRNNLVLEKPTILVAEDLTPSQFLSLDLKNLAGMILEKTGRTSHTLILARASAI
                     PVLSGLPLDAIARYAGQPAVLDAQCGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAA
                     AQLAYSRDNKRIDIAANIGTALEAPGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFE
                     AYQQVLLAAGDKPIIFRTMDIGGDKSIPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQ
                     LRAILRAASFGNAQLMIPMVHSLDQILWVKGEIQKAIVELKRDGLRHAETITLGIMVE
                     VPSVCYIIDHFCDEVDFFSIGSNDMTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIV
                     TTAHQRGKWVGICGELGGESRYLPLLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRE
                     LARQACECRSAQEIEALLTAFTPEEDVRPLLALENIFVDQDFSNKEQAIQFLCGNLGV
                     NGRTEHPFELEEDVWQREEIVTTGVGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSE
                     MGEVELVIMLTLGANEGMNHVKVFSQLARKLVNKNFRQSLFAAQDAQSILTLLETELT
                     F"
     gene            complement(2229..2854)
                     /locus_tag="ACHW42_004103"
                     /pseudo
     CDS             complement(2229..2854)
                     /locus_tag="ACHW42_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          675 a          753 c          814 g          612 t
ORIGIN      
        1 gacagcgttc agccgacgca ggcagaactg acggcgcatc cgctgccgcg ttcattaagc
       61 cgccttaacc cggatttact gtacggcaat gtgctggcaa gcggcgtcgg cgtgggtacg
      121 ctgaccctgt tacaaagcga cagcctcgac agttatcggg caatccccgc cagtgcgcaa
      181 gattccaccc ggctggagca cagcctggca acgcttgccg agcaactgaa tcagcaattg
      241 cgtgagcgtg acggcgaaag caaaactatc ctcagcgctc atttgtcgct gattcaggat
      301 gatgaatttg caggcaatat ccgtcgcctg atgacagaac agcatcaggg actgggggcg
      361 gcgatcatca gcaatatgga gcaggtttgc gccaaactat ctgcttctgc cagcgattat
      421 ctgcgcgagc gggtcagcga cattcgcgat atcagcgaac agttgctgca tatcacctgg
      481 ccggaactga agccgcgcaa caatctggtg ctggaaaaac cgaccattct ggtggctgaa
      541 gatttaaccc caagccagtt tttgagcctt gatttgaaaa atcttgcggg tatgattctg
      601 gagaaaaccg ggcgcacctc gcatacactg attctggccc gtgcctcggc gatcccggta
      661 ctgagtggct tgccgctgga tgcgattgcc cgttatgccg ggcaacctgc agtgcttgac
      721 gcccagtgcg gcgtgctggc gattaacccg aatgacgcgg tgagcggtta ttatcaggtc
      781 gcgcagacgc tggcggataa acgccaaaaa caacaggcgc aggctgccgc gcagctggcc
      841 tattcccgtg ataacaagcg tattgatatt gcggcgaata tcggcaccgc tctggaagcg
      901 ccaggcgcgt ttgccaacgg cgcggaaggt gtcgggctgt tccgtaccga aatgctctat
      961 atggatcgcg acagcgcgcc ggacgagcag gagcaatttg aagcctacca gcaggtgcta
     1021 ctggcggcgg gcgacaagcc gattatcttc cgcacgatgg acatcggcgg cgataaaagc
     1081 attccttatc tgaatattcc ccaggaagag aacccgttcc tcggctatcg cgcggtacgt
     1141 atttacccgg aatttgctgg cctgttccgc actcaactgc gggccatttt gcgcgccgcc
     1201 agtttcggca acgcccagtt gatgatcccg atggttcaca gcctcgatca gatcttatgg
     1261 gtgaaaggcg agatccaaaa agcgatcgtt gagcttaagc gcgatggcct gcgtcatgca
     1321 gagacgatta cgcttgggat catggtggaa gtgccgtcgg tgtgctacat catcgaccac
     1381 ttctgcgatg aggtcgattt cttcagtatc ggctccaacg atatgaccca gtatctgtat
     1441 gcggtcgatc gtaataaccc gcgcgtatcg ccgctatata acccgattac gccatcgttc
     1501 ctgcgcatgt tgcagcaaat agttaccact gcgcatcagc ggggcaaatg ggtaggcatt
     1561 tgcggtgaac tgggcggtga aagccgttat ctgccgctac tgcttgggct gggcctggat
     1621 gagctgagta tgagtagccc gcgtattccg gcggtgaaaa gccagcttcg tcaactggat
     1681 agcgaggcgt gtcgggaact ggcgcgtcag gcatgtgaat gccgcagtgc gcaggaaatt
     1741 gaagcgttac tcaccgcctt tacgccggaa gaagacgttc gcccactgct ggcgctggag
     1801 aatatctttg ttgatcagga ttttagcaat aaagagcagg cgatccagtt cctgtgcggc
     1861 aacctcggcg ttaacgggcg cactgaacat ccgtttgagc tggaagaaga tgtctggcag
     1921 cgggaagaga ttgttaccac cggcgttggt tttggcgtag cgatcccgca caccaaatct
     1981 cagtggatcc gtcattccag tatcagcatt gcccggctgg cgaaaccggt tgactggcag
     2041 tcagaaatgg gcgaagtcga actggtgatc atgctgacgc tgggtgctaa cgaagggatg
     2101 aatcatgtga aagtcttctc gcagctggcg cgtaaactgg tgaataaaaa cttccgccag
     2161 tcgttgtttg ccgcgcaaga tgcacaaagt atcctgacgc tgctggaaac agaattaacc
     2221 ttctgacgtt agccctgaaa acgggcgctg tactctcccg gcgtcagact aaactgacgc
     2281 cggaaaacgc gacaaaaata gtcgctatcc ggaaaaccgc aacgctgtgc cacttcatga
     2341 attgacagat gatatttctg caaaatcatc ctcgccttcg ccattcgcac ccaacgcaca
     2401 tactcgataa aacgcatcgt tccatgctga gcaaacagtt ttgacagatg attgggcgtg
     2461 atattaaaaa actgcgccac gctctcgcgg gtgagcggct gggcataatt atcctaaacc
     2521 cagttacaaa tgctgtgata gagaaaatcg ccgcgcggtt gtgaattacc cggcggcata
     2581 ttcaccacgt tgcggcaaag atgcagcaaa cttaacacca gtggctgaat gatattctgc
     2641 tccagcggcg aacggctaag atgggtgagg gcggtaagca tcgcttcacc ttcaccgcgt
     2701 tgcaggctgg gaagctgggt ctggcgagca ggatgcaaca acgacgtggt gcggctatcg
     2761 taaaacgata acccaagcca ggtcggtgcg aacaccaggc ttaacagcat caccggtttg
     2821 ttgttgatcg gtaaattagc cgcgcgtgcc ggaa
//
LOCUS       DAZSNM010000321         2852 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZSNM010000321 DAZSNM010000000
VERSION     DAZSNM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.324"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..758)
                     /locus_tag="ACHW42_004104"
     CDS             complement(<1..758)
                     /locus_tag="ACHW42_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR8945684.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPA"
     gene            complement(755..>2852)
                     /locus_tag="ACHW42_004105"
     CDS             complement(755..>2852)
                     /locus_tag="ACHW42_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR8945685.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
BASE COUNT          735 a          731 c          611 g          775 t
ORIGIN      
        1 gctggtcgcc cttcatcttt gagcatgatt tcataactga tggcattacc gttaaaacca
       61 tcgtacaggc acatgtccaa catggtcaga tcttgcgcgt agttgctgcc gttaacgctt
      121 cctgttggat gcaagtctaa ctgcaccctt ggcgtggcgt gggcgaactc cgggaagaac
      181 acgtcaatat ggttggggtc gagcgtattc agggtaatat ttgcgaaata gtcctgggcc
      241 gggctgttcg aatgtaattt aactttcccc ttccagacgc caccaatcgg caaattcttc
      301 atctctgtct gctcaaggta gagcgtaaga aactttgtat cgtatatata ataatgttga
      361 ccatcttctg tacatgaaga tctattagaa tccttaagat tcgtctgaag ccctttgccc
      421 cccttgcatc ctagcggcgc ttcatcgcta cttacaatgg ctgttttaaa tcctttgagt
      481 tttaccggcc aacgcatccc actgcgttgt tcagttaaat agatttgtac tgtcgatata
      541 tttccattag ctgtacaggt ttgttggagg tcaggatacg aacattcata ccacatatat
      601 ccattatttc ctgccgacgc ttcatccttc cagaagtaat aatccgctct accagatgcc
      661 aaatcatacg taatttcact ggtcacgtca ggagctgcct gaacgccagt gagcgtgcca
      721 aacaaaccca atatcgccgc aatcaatcga tttctcatgg tgcaacctcc ctttgcgccg
      781 tctgaacgtt tccacgtaat ttaggctgtt tcaactgtgc ctgaacctga atcgactccg
      841 gtaattgtga ataagtcacc acactacaag taacatctcc gctgtaacga atgctggcgc
      901 gtttctttac aacactcagt ggacacttaa ggaattgctg cccggaaagc agatagagct
      961 ctttttcgct gccgttaatt tcaagtgtaa atccgccatc ttcagctacc agtaacatgg
     1021 cgctgttcag ccccacaatc gggaattttt tcaccgctgc cggcagtaat aaacgaccga
     1081 tatagttgta gttaaagctg gcagacactt cgcgatagcg cagcttgcca ggcaacaaca
     1141 ttaattcacg attccctgca ccgttaatga tgtttgttgt cccctgctga ttgccctggc
     1201 tactgtcgtt aacctcaaca aaaccagact gaaatccagg ataagggaac aacacactgt
     1261 cactttgacc aagctgcaaa gttcggctgc ctgccacccg cgcttcgagt tgcagatcct
     1321 gactctcttc atcctgttca gagaagcctt tcacatccac cagtaccgca cccagcaaat
     1381 cgtctgtacc actggctccc aaattaacgc catagcgact gacagccagc gtagagctat
     1441 aagtgcccgt aaaggccgaa agatgatcat gattcttacg gtcataacta tcagatacgg
     1501 tagcgtagac atttccgtat tgcccatccc aacgcccgtt aaccgcgctg tttatggtat
     1561 cggtgttcag gcctccgacg gttacgccaa gttctttatg gctgaatgaa tcgttataaa
     1621 aggtatgcga taactgccat gacgtttgat cgccatcctg ttcgctataa cgataatccg
     1681 taggcacatt ggttgaatgg ctgttgttat tgctatccat cgttggcgtg tcagataacg
     1741 aaaacgataa gtacaagccg ttgtcgtcgt tatcgccacc gttgtcatta cgatggaaaa
     1801 caccgactga cgtactcaaa atccagtcac gcatcacaat agcgcgggaa gcgctaagtt
     1861 gtacagtttc agagatactt tgcgctgttg tctggcgaag atagttattt tcaaagctct
     1921 gctgtttatc ccaacgcaaa ctatcatcag tgttattttg tgtgcaggta taacccagca
     1981 tcatattcca gccgacaaaa ttcagtgaaa ctgtcgcgga aatactttca taacagctta
     2041 aggcgttata gctctctctg ctgtcggttg tacaagcatc gccgtcagag ttggtccgat
     2101 aaaagcccaa cgtcggccat cccggattac tccagttggc ctgttgcata tcacctttgc
     2161 cgccgtcatc gttacggaac acgctggcgc tgattgcaag attccccacc ctgcctaaat
     2221 ctgccgtcca gttattacct aactcgaaag cactcacatc atcagcattc gccagccctg
     2281 cgtagagctc atattgcgga tgtaatggca ggcgtactcc tagctgataa gctgaacttt
     2341 catcatcaga aacctgtgat gtagttttac ctgcctgtaa gaaccattgc gcatttccgt
     2401 cagtcagacc gccggttttg gtaaacggca caagctcggt gcgggtgagt tggttatttt
     2461 cgtagacttt taatgccacg ctgtagctgc ctggcggaaa ggaactggta tcaataaatt
     2521 gcgaaccact attgagataa aacgatccca aaagttgctc attacgataa gcgtcaacac
     2581 gagaattgcg cgaaagcaga accattactg gggttccttg ctggctttgc gcctggttta
     2641 aatagctcag tgtcgacccg atacgcatcc catcgattgc accgagtggc agaaagttaa
     2701 aggtaaagtt cccgccttgt gcattaaata gtgtgcggtt gtccatgcga cccgcctgca
     2761 cataataacg acgtaaaaaa tcataacgat aatagagatc gctgacgtca gtattactgt
     2821 cactgacatc atctgcatca taaccgttga aa
//
LOCUS       DAZSNM010000322         2815 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZSNM010000322 DAZSNM010000000
VERSION     DAZSNM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.325"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..1941)
                     /gene="mobF"
                     /locus_tag="ACHW42_004106"
                     /pseudo
     CDS             complement(1..1941)
                     /gene="mobF"
                     /locus_tag="ACHW42_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
     gene            2023..2250
                     /gene="vapB"
                     /locus_tag="ACHW42_004107"
     CDS             2023..2250
                     /gene="vapB"
                     /locus_tag="ACHW42_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFR8945686.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            2250..2648
                     /locus_tag="ACHW42_004108"
     CDS             2250..2648
                     /locus_tag="ACHW42_004108"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFR8945687.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
BASE COUNT          585 a          871 c          710 g          649 t
ORIGIN      
        1 cccgggcggt acatatcccg cagataacgg ctccggctgt ccagccagac cggtgacagg
       61 gcggtcatgg ttacctccgg gtgtccgagc acgccctgtg ttttcagctc actgcgaatg
      121 gcctgtgtca gtatggcctg ttcccgtact ccgctgacct gtgccacgct ctcttctccg
      181 gctttcacgc tggccgcaaa atctccggcc agccgacatt acggtccggt tcactgatga
      241 tggtggccgg tcgctgttct cccccctgcc agcgatatgt gttcaccccg gcatccttca
      301 tggccatcag tgcactgcct gtaccggttc gctgcccgct gtcggttatc aggacctgta
      361 cgttatgacg tgcggcacca tccagcaggg ttaacgtctc tttcagggag agtttttcgc
      421 cctggtcgac gataacggta ctgcccggcg tgaaggccat tccttccagc aactgacgac
      481 gtccggttat cagttcaccg gacagccgtt catcctgctt caggttcatc tgcgagcgac
      541 ggtcagcagc gataatctgc acctcccgcc cctgctcccg ggccatcatg accagttcag
      601 ccacccgctc acgctgcccg gctgcaccgc cctgcccgga cacaatggcc agcgacggac
      661 ggtcctgtgc cagaacactg acggcatcgc tgtaaccggc cgtccggggg acacttttct
      721 ccggatgtac ggtcacccgg ttctgtttca tgatgtcacg actgagtgcc cggactgaca
      781 gctcatcgag catatgaatc ccgaacgtga acagcccctt ctcacgatcg agggggataa
      841 gctgctcacg gctgatggcc tcatcgatac cggcgcgtgc ccgttcaatc acaccatttt
      901 ccggcggcag tatgccgacc gtcctggcca gcaggtccgt atacataaac tgcactttgc
      961 gttcacttaa tccggcaatg gcctgtgtca ccgcctgctg cacatccggc ccgtcctgtg
     1021 aagcgggtcc gggtgtctgc atgcgggtat atgcccgttg ttccgctgcg tcacgatatg
     1081 cccggatgtc gaacccggtt tccttcagcg tctgcatcca ctcggccatt ctgacttcag
     1141 gatcgacgtg ctgtttggat ttacgcgtat ccagcgccgc cacatcccgg gatttcagcg
     1201 aggcgtcttc cccgacggcc tcccggatag tctgtgagcg tccggaaaag gcctccaccg
     1261 gtacacccgg catttcccac ataccgtgct taccgaccac ttcagtttca tagcccagcg
     1321 cctcaacctg ctctttcagt ttttcccggt agagcctgcc aaaggcaatc tgattagcgt
     1381 acacattctc aatgaacccc gttttcccca ctttgtcact gctcagtgtc ttccattcgc
     1441 cgttatactg cgtaacatta gtcaccaccg catgcgtgtg taactgtggt tcctgatcgc
     1501 gactggtgtc gtggttaaac agtgccatca ccagattacc ggtcagcacc gtttctgact
     1561 gtccgtccgt caggtggagg cactggcctc cacctgacgg acagcaaaat ccacggcctg
     1621 gttatgtgct tcaataagac gtttgtcccc cccgagcatg gccatcatgg agatactttt
     1681 gggagcggag aaggtcaggt cgtagccggg acgatgcctg ttactgccat cctgcatgcg
     1741 gcttaaatcc gccccgtccg gcagcctgcc ctccagaagg cgggtaaaga catccttatc
     1801 gacactgccc tgcagcccca gttgttcagc cccctggccg gcccagcgtt ctcccatgct
     1861 gcccagcaca tagtaattat ccttgtcggt ataataatta ccggcacttc cggccgatct
     1921 gacctgcgcg atactcatca taaacgtata tccctttgac atatcccggt atcaatccca
     1981 caatagatat acacaagaca tatccacata aggaggcaaa taatggaaac caccgtattt
     2041 ctcagcaacc gcagccaggc ggtcagactg ccaaaagcgg ttgcattgcc ggaaaacgta
     2101 aaacgcgttg aagtgattgc tgtcggacga accagaatca ttacgccagc cggagagaca
     2161 tgggacgaat ggttcgacgg acacagcgtc agcaccgatt ttatggataa cagggaacag
     2221 cccggcatgc aagaaaggga gtcattctga tgctgaagtt tatgctcgat accaacatct
     2281 gcatttttac gataaagaac aaacccgcca gcgtcaggga gcgttttaac ctgaaccagg
     2341 ggaaaatgtg tatcagttcg gtcaccctga tggagctgat atatggtgca gaaaaaagcc
     2401 agatgcctga acgtaatctc gctgtgatcg agggatttgt ttcccgcatt gatgttctgg
     2461 attacgacgc tgctgcagcc acacacaccg gccagataag agcagaactt gcccgtcagg
     2521 gacgccctgt cgggccattt gatcaaatga tcgcaggtca tgcccgcagt cgggggctga
     2581 ttattgtgac taataacacc cgggaatttg aacgtgtggg cggcctgaga actgaagact
     2641 ggagctgacc tgttcatcag aaatcatctc ccggctcaac atcctccccg cgttcccggt
     2701 gcacattgat gttcacctct tcacgacgct gcatctgctg ccaggtgtcc ggatgatttt
     2761 cctgctgcca tgcctcataa gcggccatat ccaccacttc accggattca ctgat
//
LOCUS       DAZSNM010000323         2807 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZSNM010000323 DAZSNM010000000
VERSION     DAZSNM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.326"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            6..81
                     /locus_tag="ACHW42_004109"
     tRNA            6..81
                     /locus_tag="ACHW42_004109"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHW42_004110"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHW42_004110"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHW42_004111"
     CDS             complement(299..1057)
                     /locus_tag="ACHW42_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR8945688.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHW42_004112"
     CDS             complement(1065..2168)
                     /locus_tag="ACHW42_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR8945689.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="ACHW42_004113"
     CDS             complement(2178..>2807)
                     /locus_tag="ACHW42_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFR8945690.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          763 a          739 c          675 g          630 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaataacgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAZSNM010000324         2779 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZSNM010000324 DAZSNM010000000
VERSION     DAZSNM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.327"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..681
                     /gene="ospC1"
                     /locus_tag="ACHW42_004114"
     CDS             <1..681
                     /gene="ospC1"
                     /locus_tag="ACHW42_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HFR8945691.1"
                     /translation="DKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMKLGLGLYLIDFIR
                     KSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKKVKIREISLEEAV
                     TASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQDVISIKHELYDI
                     EYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYENAEMIKLLLKYG
                     ATSDNKYI"
     gene            complement(785..934)
                     /locus_tag="ACHW42_004115"
                     /pseudo
     CDS             complement(785..934)
                     /locus_tag="ACHW42_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1010..2707
                     /gene="ospD3"
                     /locus_tag="ACHW42_004116"
     CDS             1010..2707
                     /gene="ospD3"
                     /locus_tag="ACHW42_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR8945692.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYISVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT          980 a          461 c          531 g          807 t
ORIGIN      
        1 gataaatttt cagaggttaa taaagaagtt agtcgatacg tacatggaag taaaggaatt
       61 atagatgtac caatattcaa tactaaagat atgaagttag ggctcggatt atacctgatt
      121 gactttatta gaaaaagtga agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt
      181 gcccctgtgg atctggatag aatcataaac tttgtttttc agccagagta ccatatacct
      241 aggatggtaa gtacagaaaa cttcaaaaaa gttaagatta gagaaatatc cttagaggag
      301 gctgttacag catctaatta cgaagaaatt aacaagcagg tcactaacaa aaaaattgct
      361 ctccaggctc tttttctttc gattactaat caaaaagagg atgtcgcctt atatatatta
      421 tctaattttg agataactag acaagatgtt atttccataa agcatgagtt gtatgatatt
      481 gagtatctac ttagcgctca taattcaagc tgtaaagtac ttgagtattt tatcaataag
      541 ggattggttg atgtaaacac aaagttcaaa aaaactaata gtggggattg tatgttggat
      601 aacgcaataa aatatgagaa tgcagaaatg ataaaactat tattgaaata tggtgcaaca
      661 tctgacaata aatatattta atcaaaattg aatatcgttt agcaaaaggc agtatttgca
      721 caaaatatcg atatattgtt tattgtcagt atggctcaat gtgataatag ttggaaagtt
      781 tgatgggttt cgccccgttg tagcggtagt cgaactggaa ggtcttcagg cactgcttac
      841 agcgatagag cagcccccct gaactggaat ggccgttccg gatacccccc tgagtttcag
      901 agtaacggtg acaaaccaca tcaatctttg ccatcaatca tccaaagggc aaagagtaca
      961 acaacactaa gtctgcgtca caacccatca atgaaaggaa tatatacata tgccatcagt
     1021 aaatttaatc ccatcaagga aaatatgttt gcaaaatatg ataaataaag acaacgtctc
     1081 tgttgagaca atccagtctc tattgcactc aaaacaattg ccatattttt ctgacaagag
     1141 gagtttttta ttaaatctaa attgccaagt taccgatcac tctggaagac ttattgtctg
     1201 tcgacattta gcttcctact ggatagcaca gtttaacaaa agtagtggtc acgtggatta
     1261 tcatcacttt gcttttccgg atgaaattaa aaattatatt tcagtgagtg aagaagaaaa
     1321 ggctattaat gtgcctgcta ttatttattt tgttgaaaac ggttcatggg gagatattat
     1381 tttttatatt ttcaatgaaa tgatttttca ttccgaaaaa agcagagcac tagaaataag
     1441 tacatcaaat cacaatatgg cattaggctt gaagattaaa gaaactaaaa atggggggga
     1501 ttttgtcatt cagctttatg atcccaacca tacagcaact catttacgag cagagtttaa
     1561 caaatttaac ttagctaaaa taaaaaaact gactgtagat aattttcttg atgaaaaaca
     1621 tcagaaatgt tatggtctta tatccgacgg tatgtctata tttgtggaca gacatactcc
     1681 aacaagcatg tcctccataa tcagatggcc tgataattta cttcacccca aagttattta
     1741 tcacgcgatg cgtatgggat tgactgagct aatccaaaaa gtaacaagag tcgtacaact
     1801 atctgacctt tcagacaata cgttagaatt acttttggca gccaaaaatg acgatggttt
     1861 gtcaggattg cttttagctt tacaaaatgg gcattcagat acaatcttag catacggaga
     1921 actcttggaa acttctggac ttaaccttga taaaacggta gaactactaa ctgcggaagg
     1981 aatgggagga cgaatatcgg gtttatccca agcacttcaa aatgggcatg cagaaactat
     2041 caaaacatac ggaaggcttc tcaagaagag agcaataaat atcgaataca ataagctgaa
     2101 aaatttgctg accgcttatt attatgatga agtacacaga cagatacccg gactaatgtt
     2161 tgctcttcaa aatggacatg cagatgctat acgcgcatac ggtgagctca ttcttagccc
     2221 ccctctcctc aactcagagg atattgtaaa tttgctggcc tcaaggagat atgacaatgt
     2281 tcccggactt ctgttagcat tgaataatgg acaggctgat gcaatcttag cttatggtga
     2341 tatcttgaat gaggcaaaac ttaacttgga taaaaaagca gagctgttag aagcgaaaga
     2401 ttctaatggt ttatctggat tgtttgtagc cttgcataat ggatgtgtag aaacaattat
     2461 tgcttatggg aaaatacttc acactgcaga ccttactcca catcaggcat caaaattact
     2521 ggcagcagaa ggcccaaatg gggtatctgg attaattata gcttttcaaa ataggaattt
     2581 tgaggcaata aaaacttata tggaaataat aaaaaatgaa aatattacac ctgaagaaat
     2641 agcagaacac ttggacaaaa aaaatggaag tgattttcta gaaattatga agaatataaa
     2701 aagctgaata ttatctcgtg gtgatacact gaatttgagg taaccggctc atttaaaccg
     2761 tctggtctgt tacctccgg
//
LOCUS       DAZSNM010000325         2762 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZSNM010000325 DAZSNM010000000
VERSION     DAZSNM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.328"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..482
                     /locus_tag="ACHW42_004117"
     CDS             <1..482
                     /locus_tag="ACHW42_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR8945693.1"
                     /translation="MQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFV
                     YKESCTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVP
                     LSVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            831..2003
                     /locus_tag="ACHW42_004118"
     CDS             831..2003
                     /locus_tag="ACHW42_004118"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR8945694.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2057..>2762)
                     /locus_tag="ACHW42_004119"
     CDS             complement(2057..>2762)
                     /locus_tag="ACHW42_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005078762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945695.1"
                     /translation="CEGNGTQGDSSTGGAQLAINGLNGSCALVLNNPYFEANAGGADL
                     AIDNTGTRPVTVVINGGNFHRVSSARYTHTNIQVTSSGGGKVTVILNGTTFQSAGDYQ
                     PSAEQPYWITGANCEVVDIGCVFTETTSKVTSASALSVTRSGKINANGSIDVATGVSS
                     VNVVSTGVYDVSFSHPLAAVASGYIVQITPISAPDSVSCDVTYIGVDTFRVTLRNTLS
                     GAGISSSFAFSITRLI"
BASE COUNT          851 a          572 c          537 g          802 t
ORIGIN      
        1 atatgcagcg ccgggtggca tggataaacc cggaagagag taaatcaaac cgcgcaattg
       61 gcgttgcgct gaatgatact gcatgtcgcg tattgaaaaa acaaatcggg aatcatcacc
      121 gttgggtatt tgtgtacaag gaaagctgta ccaaaccaga cggaacgaaa gcgccaacgg
      181 tcaggaagat gcggtatgac gcaaacacag cctggaaagc ggcgctgaga cgagcaggta
      241 ttgatgattt cagatttcac gacttgagac acacctgggc aagttggctg gttcaagccg
      301 gagtcccgtt gtcagtgtta caggaaatgg gaggctggga gtctatcgaa atggttcgtc
      361 gatatgctca ccttgcacct aatcacctta ccgaacacgc acggcaaata gactcgatcc
      421 tgaacccatc ggtcccaaat tcgtcccagt caaaaaataa ggaaggtact aatgatgtgt
      481 aacttattga tttaaatggt gccgataata ggagtcgaac ctacgacctt cgcattatga
      541 atctgtagca ccaatcataa ctatctgttt tagcaagcat taaccgcatt cactaagcta
      601 tagttgatga cacaaacaga aagttgatgc atgatgttgc catatatatg tcacaaatac
      661 ggcacaacga tcttcaaaca tatagccaca cattcacaga agagcacaaa gccttgcaat
      721 ccagtgcaaa gcttggtgtg tctccgtttt gtttcatcag ttacacataa ctatatgatc
      781 attaactaaa agtcagtata cttggagaaa ttttaattgc gtaatagagc atgcatatga
      841 ttgaaattaa cagtttatta ttaataacat ccgtgatatt gatgtcgtta ttagctgtgg
      901 ggttatttga taaaatttcc ccaataaatc ttgttgagca cggcaggaat aatcaaatag
      961 atggaatgcg ggggttctta gcaattttcg tgcttattca tcacgcagca atttggaatg
     1021 gatacttgtc atctggagta tgggaagcac cttcatcaaa tctgttagca aatttaggcc
     1081 aagttggtgt gtcattcttt tttatgatta ctggttatct gttcttttca aagattatct
     1141 ctggagatca ggactggaca aggctttatg tatcaagatt actacgatta accccaatgt
     1201 tcatagttag cctatgtctc atttttatca ttgtaggttt taagtctgga tggagaatgc
     1261 aggtatccac agaagagctt tttgtgtcaa taatgaagtg gttgccattc actgcgctag
     1321 gtatgccgaa cattaatgac gtaaaagatt catttactat caatgccgct gtaacatgga
     1381 cacttgtata tgaatggttc ttttattttt ctcttccggt aatttccgcg ctcataaaaa
     1441 gaaaagtcag tatttacatg gttatgatta gcgcaatatc gctatttgtt ttcattttat
     1501 ttttcagtaa aatacacata gcatcatttc tttttgggct cctggcattt ttgctaaata
     1561 aatcaaagat agtcaatgga attgccaaag caaaagtaac accaattata ataactgcga
     1621 taatggtttt tgaaatgacg tatttcaaaa caacttacgc accgctacca ctgattcttt
     1681 gtggaataac atttatcatt atcgcatcag gttgtgattt gtatggaata ttaagattaa
     1741 atataaccag aaagctagga gaaactacct acagcgttta ccttctgcat gggatattcc
     1801 tttattgcct aatgacgtgg attattccta ataattacac tgaaaataca tttatcatac
     1861 tggtaagcac aactgcattt cttattacgt tcacatcatg cctaactttt aaattaatag
     1921 agacgccatt catcaaatta acaaaacaaa ccacaacttt agtaaaagaa ttaataccca
     1981 cattaacaaa caacaatcaa taaacaataa acataagccg ccctacatat atgtccaaat
     2041 ggggcggcga atgtatttat attagtctgg taatgctaaa tgcaaacgaa ctggagattc
     2101 ctgccccgct aagcgtattg cgcagggtca ccctaaatgt atctacgcca atataagtaa
     2161 catcacaact aactgaatca ggcgcagata taggtgttat ctgcacaata taaccgctag
     2221 caactgctgc gagcggatga gaaaagctaa catcatacac acctgtagaa accacgttaa
     2281 ccgatgaaac accagtagca acgtcgatac taccattggc gttaattttt cccgagcgtg
     2341 tgactgacaa tgcagaagcc gatgttacct tactggttgt ttctgtaaaa acacacccaa
     2401 tgtcaactac ctcgcaatta gctcctgtta tccagtatgg ttgttctgca cttggctgat
     2461 aatcccctgc gctctggaac gttgtgccgt tgagtatgac tgtaactttt cccccacctg
     2521 agcttgttac ttgaatattc gtgtgcgtgt atcttgcact gctcacgcga tgaaaatttc
     2581 caccgtttat aaccacagtt acagggcgcg tcccagtatt atctatagca aggtctgccc
     2641 ccccggcatt agcctcaaag tatgggttgt ttaataccaa agcgcacgaa ccgttaaggc
     2701 catttatagc taattgcgca ccacctgtac tggaatcccc ctgagtacca tttccttcac
     2761 aa
//
LOCUS       DAZSNM010000326         2735 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZSNM010000326 DAZSNM010000000
VERSION     DAZSNM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.329"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHW42_004120"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHW42_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR8945696.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="ACHW42_004121"
     tRNA            complement(621..695)
                     /locus_tag="ACHW42_004121"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="ACHW42_004122"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="ACHW42_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..>2735
                     /gene="mlaA"
                     /locus_tag="ACHW42_004123"
     CDS             1995..>2735
                     /gene="mlaA"
                     /locus_tag="ACHW42_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR8945697.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKD"
BASE COUNT          639 a          674 c          668 g          754 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagat
//
LOCUS       DAZSNM010000327         2732 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZSNM010000327 DAZSNM010000000
VERSION     DAZSNM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.330"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            217..1182
                     /gene="dusB"
                     /locus_tag="ACHW42_004124"
     CDS             217..1182
                     /gene="dusB"
                     /locus_tag="ACHW42_004124"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR8945698.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1208..1504
                     /gene="fis"
                     /locus_tag="ACHW42_004125"
     CDS             1208..1504
                     /gene="fis"
                     /locus_tag="ACHW42_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR8945699.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1590..2474
                     /gene="yhdJ"
                     /locus_tag="ACHW42_004126"
     CDS             1590..2474
                     /gene="yhdJ"
                     /locus_tag="ACHW42_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR8945700.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2558..>2732
                     /locus_tag="ACHW42_004127"
     CDS             2558..>2732
                     /locus_tag="ACHW42_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR8945701.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
BASE COUNT          761 a          630 c          660 g          681 t
ORIGIN      
        1 gaaaaattga gaacttactc aaatttcttt gagtgtaaat tttagtcact attttctaat
       61 atgatgattt ttatgagtaa ttatcgcacc acgctcattt taaatgcaat tctttgatcc
      121 atctcagagg attggtcaaa gtttggcctt tcatctcgtg caaaaaatgc gtaatatacg
      181 ccgccttgca gtcacagtat ggtcatttct taactcatgc gcatcggaca atatcagctc
      241 agaaatcgcc tgatcgcagc gcccatggct ggcattacag acagaccttt tcggacgttg
      301 tgctacgaga tgggagccgg attgacagta tccgagatga tgtcttctaa cccacaggtt
      361 tgggaaagcg acaaatctcg tttacggatg gtgcacattg atgaacccgg tattcgcacc
      421 gtgcaaattg ctggtagcgc tccgaaagaa atggcagatg cagcacgtat taacgtggaa
      481 agcggtgccc agattattga tatcaatatg ggttgcccgg ctaaaaaagt gaatcgcaag
      541 ctcgcaggtt cagccctctt gcagtacccg gatgtcgtta aatcgatcct taccgaggtc
      601 gtcaatgcag tggacgttcc tgttaccctg aagattcgca ccggctgggc accggaacac
      661 cgtaactgcg aagagattgc ccaactggct gaagactgtg gcattcaggc tctgaccatt
      721 catggccgta cacgcgcctg tttgttcaat ggagaagctg agtacgacag tattcgggca
      781 gttaagcaga aagtttccat tccggttatc gcgaatggcg acattactga cccgcttaaa
      841 gccagagctg tgctcgacta tacaggggcg gatgccctga tgataggccg cgcagctcag
      901 ggaagaccct ggatctttcg ggaaatccag cattatctgg acactgggga gttgctgccc
      961 ccactgcctt tggcagaggt taagcgcttg ctttgcgcgc acgttcggga actgcatgac
     1021 ttttatggtc cggcaaaagg gtaccgaatt gcacgtaaac acgtttcctg gtatctccag
     1081 gaacacgctc caaatgacca gtttcggcgc acattcaacg ccattgagga tgccagcgaa
     1141 cagctggagg cgttggaggc atacttcgaa aattttgcgt aaacagaaat aaagagctga
     1201 cagaactatg ttcgaacaac gcgtaaattc tgacgtactg accgtttcta ccgttaactc
     1261 tcaggatcag gtaacccaaa aacccctgcg tgactcggtt aaacaggcac tgaagaacta
     1321 ttttgctcaa ctgaatggtc aggatgtgaa tgacctctat gagctggtac tggctgaagt
     1381 agaacagccc ctgttggaca tggtgatgca atacacccgt ggtaaccaga cccgtgctgc
     1441 gctgatgatg ggcatcaacc gtggtacgct gcgtaaaaaa ttgaaaaaat acggcatgaa
     1501 ctaattcagg ttagctaaat gcttgattaa aaaggcgcta ctcggcatgg ggaagcgcct
     1561 tttttatagg tgtcacaaag ggagtgacca tgagaacagg atgtgaaccg acccggtttg
     1621 gtaatgaagc taagaccatt attcacggtg atgcctttgc cgaacttaaa aagctaccta
     1681 ctgaaagcgt cgatctgctc tttgccgacc caccgtataa catcggtaaa aattttgatg
     1741 gtctgatcga agcctggaaa gaagatctgt ttatcgactg gctgtttgaa gtgattgcag
     1801 agtgccaccg cgttctgaaa aagcagggca gcatgtacat tatgaacagt acggaaaaca
     1861 tgccctttat cgatctccag tgccgcaagc tttttaccat caaaagtcgc atcgtctggt
     1921 catatgacag ttctggagta caggcgaaaa aacactacgg ctccatgtac gaacccatcc
     1981 tgatgatggt gaaagacgca aagaactaca cattcaacgg tgatgctatt ctggtagaag
     2041 ccaaaaccgg atcgcagcgc gcgttgatcg attatcgcaa aaatcctcca cagccataca
     2101 atcatcaaaa agtaccgggt aacgtctggg attttccgcg cgtgcgttat ttgatggatg
     2161 aatatgagaa ccacccgacg caaaaaccga aagccttact gaaacgcatt attctcgcct
     2221 cttccaaccc aggcgatatc gttctcgacc cgtttgccgg tagctttact accggtgccg
     2281 tagccatcgc cagcggacga aaattcattg gtattgagat caacagcgag tacatcaaaa
     2341 tggggcttcg acggctggat gtcgcgtcgc attactccgc ggaagaactg gcgaaagtga
     2401 aaaaaagaaa gaccggcaac ctgtcaaaac gaagccggtt aagcgaagtt gaccccgatc
     2461 tcattgcaaa gtaaaggaag tgtaagccta gttttcagat tattcatttc gtgtatattt
     2521 cctgccagac ttggttaaac atgcacaggc tcaggtaatg attcgcaagt attggtggct
     2581 cgtcgttttc gctgtcttcg tttttctgtt tgatacttta ctgatgcagt ggattgaact
     2641 gctgacaaca gaaacagaca aatgccgtaa tatgaactca gttaatccac taaaactggt
     2701 taactgtgac gaactgaatt ttcaggacag aa
//
LOCUS       DAZSNM010000328         2729 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZSNM010000328 DAZSNM010000000
VERSION     DAZSNM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.331"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..336)
                     /locus_tag="ACHW42_004128"
     CDS             complement(<1..336)
                     /locus_tag="ACHW42_004128"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFR8945702.1"
                     /translation="MYTESNIKLSCNALWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLIADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLM
                     VLLLNNDVWC"
     gene            complement(364..926)
                     /locus_tag="ACHW42_004129"
                     /pseudo
     CDS             complement(364..926)
                     /locus_tag="ACHW42_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(990..1336)
                     /locus_tag="ACHW42_004130"
                     /pseudo
     CDS             complement(990..1336)
                     /locus_tag="ACHW42_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(1443..2006)
                     /locus_tag="ACHW42_004131"
     CDS             complement(1443..2006)
                     /locus_tag="ACHW42_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFR8945703.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
BASE COUNT          843 a          606 c          541 g          739 t
ORIGIN      
        1 acaccagaca tcgttattaa gtagcagcac cataagataa ggagtggcat cgttagccac
       61 agcttcccta ctccagaggt aatataaagg ggtgggctcg actgatttat ctttacgtcg
      121 cttacactgc aaatgttcag aaattagtct atgcagctca ccagtaaaat cagcaatcag
      181 agaaggaatt gccttattca taccagggca aggtattaat ttaaactgta ataatttaat
      241 tttaggatgt gtagcagcag cccgatacag agttgcaagg acacactttt gccagagggc
      301 gttacaggaa agcttaatgt ttgattctgt atacataata aatcacctta tagttacaat
      361 aggtcaaaaa ccgctgtagc caaagttacg ctggcctgat gctttagtgt cgagcttcgt
      421 caaataatcc agacgtacca ataagcgctg gtactgctct gggaaattag gatcgttaat
      481 atccaggatg taacgtccat tagcagggaa atgaactaat ccctggtgat cttccagttc
      541 caaccctaag gcactgtacc aggctccggt aattaaccct ctcagggtgt cagagcgctc
      601 atagtttccc agatggtaat aggcgtcttt gttaaaaaaa ggcagacgtg ataatgacat
      661 ttaaaggact gcgtatattc tttcgcccag ataatacaaa ccgacgttgc gtaggtgcgt
      721 ttgccttgtt gttttttacg gtgttgatct gcattaattt tcgcattcag cgagttacgg
      781 aaacgagata tctctccggg gatcaggtta gggaaacagg taatgatatc cccgttgtca
      841 atcacaggag ggtaatggag atccccccgt attgctgtta ttctggaagt gtttttaata
      901 ttcgtattga tagttctcag agatatatca cactctagat attttaatta agtatcagtt
      961 cgatatcggc atattgtaca ctacctcagg tagatgatga gatcagatac agggatcaac
     1021 tgacggtgag cgcagtcccc acattttatc cagggcttac tgcatattcc caacagccat
     1081 tcgttagcca ggtagacgaa taccctgatt tgccgctggt tttgctttcc tatcgaaggg
     1141 cccatacatc atcacgccca cgaaacaacc gacgaaataa cgcaactttc tcatccgtgg
     1201 ataatacgaa aacgcactgc acagggctct gcggtttacg tcgccattca atcccatgag
     1261 cttcaagtaa tgagaccagc cgggtatttt ctgcccgcag tactgctagt tcatcgctgt
     1321 cagatttata aaccatatgc gttatcactt gtacctgtct cccggcggca ccacgactga
     1381 aattcgtgtg aaggcaaatc cacgcgataa cttacgatag ttcggacata agcccccata
     1441 attcagttat gtcgtggtcg accccggtta cttttttcgg gaagcctgct acggcccaga
     1501 ataataccgt gttctttctg atcctgttta gccagatagc taagggcgta acgtaagtcg
     1561 tctaccgcag atttatcact atagtgaatc acgtgatcga tacgcaccgg gtatttgtct
     1621 ttagcccggc acagatgaaa ataaccctcc ccttccgtga tccgcctcca gatatctccc
     1681 agttgcctgg atatctgata agagttcttg tgacgttgtc catccaggta gccgataaaa
     1741 tgaatatgaa gccctttgtc tggtgtatat tccattaccc agtaatatcc cgccaacatc
     1801 gtctgggttt cgctaagtaa acggtatatt tccatacaca tactgtgctt acaggaatgc
     1861 ccgaaactgg gcgtgtcttt cctgtaagca aaatcaattc tgaaaggtaa cagtttagaa
     1921 aaacgttgaa acataccatc catgtgttca ttcacgtctt tcagaatcat gaaatccatt
     1981 ttgtaattgg gattggcatt atacatttta tagtccttac tttataaaac tcacaggaag
     2041 agagaattac gtaaatactg agagtagaga aacgccgtac cacggcatta ataaacgaag
     2101 taaaccagca gcacagacaa cctgataaaa ctgttttgta ttaccgcatt actgtgatga
     2161 ggtaatactt cagaatacag tgagactctc ttaaactgag aaccagtacg aattacaatg
     2221 gaaaaacaca cctcatagaa ttgagtttca ggaagcacca ctaaattctt tacaggaagt
     2281 aatctgacat tcacaaaact cagtatcggc tagctacagg caacatcgct attaatattg
     2341 tcgaaccatt aagtaaagcc gagagagacc acgggtattt aatcattagc ataaaagaat
     2401 aatggtatac agaacatacc cacacaccat aagaagattg ttccatacag aaaagatcat
     2461 atttacctat acctataaac tgtaacggag tcataacacg ctctaatagt gttccgatac
     2521 gtaaccagtg ttaagtacgt acaggacaac attacacaca taattcacca tacagcttcc
     2581 cccatatgct tcactgcgta gtgtatggag atatatgcat tacacacata cagaatactt
     2641 agcataaaga ggagtgacat taccacgtaa agagataagt tatacaacaa acatacaaac
     2701 aatacggcac agacacacaa taccataac
//
LOCUS       DAZSNM010000329         2727 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZSNM010000329 DAZSNM010000000
VERSION     DAZSNM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.332"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(348..1127)
                     /gene="ipaJ"
                     /locus_tag="ACHW42_004132"
     CDS             complement(348..1127)
                     /gene="ipaJ"
                     /locus_tag="ACHW42_004132"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFR8945704.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(1324..2133)
                     /locus_tag="ACHW42_004133"
                     /pseudo
     CDS             complement(1324..2133)
                     /locus_tag="ACHW42_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2480..>2727
                     /locus_tag="ACHW42_004134"
     CDS             2480..>2727
                     /locus_tag="ACHW42_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8945705.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
BASE COUNT          814 a          686 c          442 g          785 t
ORIGIN      
        1 atccataccc ctcatagggc ttacagttta aattgcgggt ataaaataca tctattttca
       61 aataaaataa agcatactcc aattacaacg agtgactaaa ataaaaatat catccaagtc
      121 aatcactgtt tgctctgcaa aatatgtcag ccttctcaaa cttaccagca cttaaatatt
      181 taagcacaca atcattcctt gagggagttc acttatttca agagaaacat ttcgtcttcc
      241 cacacaacta atccgcccaa tatagctaaa tttgtactca gtaatatcat ttgttatgcc
      301 ccccacccca aaatattcaa gctccttcac agataaaaaa cgacaattta caaagcctca
      361 ttagttataa ctatggaaat acctatttta gtatatccta taaagttaga attacttctt
      421 cttgcaccta attccatagt tgaaaaacaa gaataatttt ctcctacagc tggatccata
      481 tagcttccat caggtctaca caacacccag tggaggccga caggaactcc aataaaggat
      541 acagcaacag cttccaaaac tctttggtta gaagataaca catctcgttg atgtactata
      601 ttacatccca tgccaattaa taagtccctt gcatcgggat aaataaaact aattacttgc
      661 gaaaaaatgt tactttcttc aacgacataa gcattcaatc caagaagcct cgtagccatg
      721 actatcttat caggcataga ataaccggca tccgcgatat tatctttgtg tattctcgga
      781 ttataattac cactagttat taagtataaa tcccgctcac aacgattatc caaatcaaga
      841 gaactcttcc ttgtcatttc ggacatagac cctttgtatt gtggtatttt atcaacccct
      901 aattcctttg cagcacagag taaactggca gctccacaag aattgtcata catctgcttc
      961 ctcgccaaaa aacaaccttg ttcgtttact ctattctcat ttaccctcac atcagtctga
     1021 tgagatatca tatattctct tggtatagcc ccctgcaata aaaccccata taacataaca
     1081 cctgtatgaa tacatccccg tttgcaaggc ttccgttgtt ccgacatcga cgagtaattg
     1141 cagcacagta ttgcatccag caatgcgaac tactatgaga attagctgac cgggtcctta
     1201 ttccccgaac gaactttaaa tccaaaacct catactcttt tccgtcgtca tcacgtccgt
     1261 caccataaca acctttcagc atccaccctt ttattaagag ttaatgtcca tcagcatcag
     1321 caattaatac cgcctgatac tgacatattt ttgtcgtatt tacgtccata atcggttatt
     1381 gtgatacagg tcacaccagc ttatttgcta ttgttacaat aaccaccttg gttgagagct
     1441 gttttttcaa ctttgttatc aactccccca actccttggt cagtaaggct gccgaccgag
     1501 cacagtgaat agatagtatc cgcaggtacc accatgctta cttaatactc agaaggcgaa
     1561 gtgctctcca attaactctg ccgcactatg cttataagct gcttttcaat gccgtctatc
     1621 aacatgtcca attcattcaa tctgttgtac tggtaatcca acgcagtaat gatatatgca
     1681 ggtattcttt catccactcc agtaacgttt catgcaaagc attaatttac gcgctacgga
     1741 acttcaccag ttgactgagc aaacgatgtg ggaccagtac tgcctgcagt tcttctattg
     1801 ttacagcgat ttctttacca aacagctgaa ccaccatcaa gataaagata gcctgaacat
     1861 tcatcgcacc attttgttac caatggcaaa agatttaacg aaccgtgcct gcatcaggcg
     1921 gactttttat ccctgctttg ttagctccct aaccttgtac agagagcctc cataagctcc
     1981 catatcaagc agacatggtt cgcagttact gacatacacc agaaaactct gacagcagac
     2041 tcacttatcc tccacttcac tcatatcctt attggtgaga tgaacctgaa tgacatgttt
     2101 tgcgacataa acgccaacca gtgtatattt cacctgtaga gattctacaa tctgtagcgt
     2161 tatgcatcta atgttaggct ctatgatgtc agaaatctgc gagactctac accttctgca
     2221 tttaccttta tttatcacca tgctcctcag ttaacggaga acgtgttcat tgcattttgt
     2281 cacttacatt tcgcctttgg tatcgggggg atataggttc caacgttccg catgagaacc
     2341 tgaaccggct tcacgccacc ttgataccag atgccgcaca ggtagtcaac aagtgtcacg
     2401 aacggtgcaa tagtgatcca cacccaacgc ctgaaatcag atccaggggg taatctgctc
     2461 tcctgattca ggagagctta tggtcacttt tgagacagtt atggaaatta aaatcctgca
     2521 caagcaggga atgagtagcc ggacgattgc cagagaactg gggctctccc gcaataccgt
     2581 taaacgttat ttgcaggcaa aatctgagcc gccaaaatat acgccgcgac ctgctgttgc
     2641 ttcactcctg gatgaatacc gggattatat tcgtcaacgc atcgccgatg ctcatcctta
     2701 caaaatcccg gcaacggtaa tcgctcg
//
LOCUS       DAZSNM010000330         2727 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZSNM010000330 DAZSNM010000000
VERSION     DAZSNM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.333"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1594
                     /gene="mppA"
                     /locus_tag="ACHW42_004135"
     CDS             <1..1594
                     /gene="mppA"
                     /locus_tag="ACHW42_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR8945706.1"
                     /translation="TCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDEPASLDPA
                     KAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNAKWADGTP
                     VTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTAVDAHTLK
                     IQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERVVNEKLVV
                     VPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKLLKDIPGQ
                     VYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPAWHFIPDV
                     TAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQKIAIAVA
                     SMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLLTSMHSGN
                     ISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNGRLIKPWL
                     KGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1645..2676)
                     /gene="ynaI"
                     /locus_tag="ACHW42_004136"
     CDS             complement(1645..2676)
                     /gene="ynaI"
                     /locus_tag="ACHW42_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR8945707.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT          790 a          622 c          644 g          671 t
ORIGIN      
        1 tacgtgttgt gcgctgttgg tcagcagcat ttctctttcg tatgctgcag aagttccgag
       61 cggcacagta ctggcagaga agcaggagct ggtgcgccac attaaagatg agcctgcgtc
      121 gctggatccc gctaaagccg tgggcctgcc agagattcag gtcattcgcg atctgtttga
      181 aggtctggtg aatcagaacg aaaaagggga gattgtcccc ggcgtcgcga ctcaatggaa
      241 aagtaatgac aaccgtatct ggacttttac cctgcgcgat aacgcaaaat gggcggatgg
      301 cacaccggta acggcgcaag attttgtcta cagctggcaa cgtctggtgg acccaaaaac
      361 attgtcgcca tttgcatggt ttgccgcgct ggcgggaatc aacaacgcac aggcgattat
      421 tgatggtaaa gctacgcctg atcagcttgg cgtcaccgca gttgatgccc atactttgaa
      481 aattcagctt gataaaccgt tgccgtggtt tgtgaattta accgctaact ttgccttctt
      541 cccggtgcaa aaagccaacg tagaaagcgg taaagagtgg acgaaacccg gaaatctgat
      601 cggcaatggc gcttatgttc ttaaagagcg cgtagtcaat gaaaaactgg tcgtggtacc
      661 gaatacccat tattgggata acgccaaaac ggtgctgcaa aaagtgacct tcctgccaat
      721 taatcaggaa tccgcagcca ctaagcgtta cctcgcgggg gatattgata tcaccgaatc
      781 cttcccgaaa aatatgtatc agaagctgtt gaaggatatt ccggggcagg tttatacgcc
      841 accgcagctc gggacctatt attatgcctt taacacgcaa aaagggccga cggcagatca
      901 gcgcgttcgc ctggcattaa gtatgacgat agatcgccgc ctgatgaccg aaaaagtgtt
      961 ggggacgggc gaaaagccag cgtggcattt tataccagat gttaccgccg gatttacgcc
     1021 ggaaccttcg ccgtttgaac aaatgagtca ggaagaactg aatgcgcagg caaaaacttt
     1081 gttgagcgca gctggttatg gtccgcaaaa accgctgaag ctgacgcttt tgtataacac
     1141 ttcagaaaac catcaaaaaa ttgcgattgc tgtagcatcg atgtggaaaa agaaccttgg
     1201 cgtagatgtt aaattgcaaa atcaggaatg gaaaacctat atcgatagcc gtaacaccgg
     1261 caattttgat gttatccgcg cctcatgggt gggggattat aatgaaccct ccactttcct
     1321 gacattatta acgtcaatgc attcaggaaa tatttcacgc tttaacaatc cggcatacga
     1381 caaagttctg gcccaggcat cgacggaaaa taccgttaaa gcgcgtaatg ccgattacaa
     1441 cgcggcagaa aaaatcctca tggagcaagc accgattgca ccaatttatc aatataccaa
     1501 tggacgatta atcaaaccgt ggctgaaagg ttatcccatt aataaccctg aagatgtagc
     1561 gtacagtcgg actatgtata ttgtgaagca ttgatgtgat gggaactggc gttacccttg
     1621 tggataacgc cagtgatgtc tgatttagcg cccctgttcc ggcggtgtaa tgttatccat
     1681 atacagcgtc tggctgggga aggcaaagtc cgcgccgtgt gactgtacaa tatcgataat
     1741 cttcaaataa acgtcttgct gtgcagcaag ccactcagcc cataccgtgg ttttggtaaa
     1801 gcaataaacc ataatattca atgacgagtc agcaaactgg ttgaaataaa ccagtaaggt
     1861 ttgtcgctgg tcgatggccg ggtgattttt cagcatctca cgtacggctt cgacaataac
     1921 gcccactttt gccgcatcct cataacgtaa accaatggtc gtggtaatgc ggcggttggt
     1981 cattcgtcct gggttttcta cgctgatcga cgaaaacagc gagttcggta cgtacaatgg
     2041 acgattatca aaggtcgtaa ttttggtaat tcgccagcca atttccgcta ctgtaccttc
     2101 gatatttctg tccggtgaac ggatccagtc gccaatactg aaaggacggt cgaaatagag
     2161 catgatcccg gaaaagaaat tactcagaat atctttaccg gccataccga cagccagacc
     2221 accaatacca ccaaaggtca gcaagccaga aaggctcatg ccgaaatgtt cgccataaag
     2281 cagaacaagc actacaataa tggtgatttt gataatgcgc gacataatcc gcgcactggt
     2341 gatatcgcga ccttttttaa tctgctgttt ttcaaactga ttaatcagca gaaatagctt
     2401 aatcgtcaga ataaccgcaa tcagggacgt acagataaaa tcgataacgc ctggggtgat
     2461 aaatttgagt ttatagtttt ctataacata attactgatg ctaccaacag cactgataat
     2521 tatggtgtag attaaaaatt gcaccgcatg gaataaaaat ccttttcttt tacgatttcc
     2581 acggcgaaac caaaagctca tcagaatcaa tgctgcgcag ctaccgaaaa taatgaccag
     2641 attaagcgca ttatttgtaa acagttcagc gatcattgtt ttatcaggct cctccagata
     2701 attgtcgtca tgccggaaac ccctggc
//
LOCUS       DAZSNM010000331         2714 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZSNM010000331 DAZSNM010000000
VERSION     DAZSNM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.334"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            53..2575
                     /gene="lpfC-O113"
                     /locus_tag="ACHW42_004137"
     CDS             53..2575
                     /gene="lpfC-O113"
                     /locus_tag="ACHW42_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR8945708.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2586..>2714
                     /locus_tag="ACHW42_004138"
     CDS             2586..>2714
                     /locus_tag="ACHW42_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8945709.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          727 a          586 c          678 g          723 t
ORIGIN      
        1 cagtgacccg ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac
       61 gaccagaata gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat
      121 ggccgaggtc agtgtattca atccggcgct tctggaaatc aaccatcaat ccggagtcga
      181 tattcgccag tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt
      241 tatcaacggt aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc
      301 tgatctgcac gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt
      361 tgatgcgctc aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg
      421 tattgaaggc tcatcctggc agtttgacag tgataaattg cagctgaata tatccattca
      481 ccaaatctac atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg
      541 gattaatgcg ctcaccatca actacgattt ttctggttca catacactac gttcagatta
      601 tggttcacaa gagacagata ccagttatct caatctgcgc aatggactga atattggacc
      661 gtggcggcta cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc
      721 cattagtacc tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga
      781 tacgtggacg gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac
      841 tgataacgat atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc
      901 aaagtccaac gccaccgtca tcattcggca gaatggctac gtgatttatc agtcagccgt
      961 tccacaaggt gcttttgaga tcaccgatct caacaccgca agtacaggtg gcgatttgga
     1021 cgtaaccatc aaagaagaag acggtagcga acaacgattc acccaacctt atgcttcatt
     1081 ggcgattctt aaacgtgaag gtctgacaga tgttgatgtc agcgtgggtg aattgcgcga
     1141 tgaagacgga tttacaccgg acgtccttca ggcgcaaata cttcatggtt tttcccacgg
     1201 gatcacttta tatggaggta tgcaggctgc tgaaaattat ggttctgcag ctctgggtgt
     1261 cggtaaagat cttggcgctt tgggcgcaat ttctttcgat gtgacacatg ctcgtgcgaa
     1321 ttttagccat gatgatacag aaacgggtca gtcatatcgc tttctctatt caaaactatt
     1381 tgacgacaca gacactagct tgcgcctggt tggctatcgt tactccaccg agggctacta
     1441 taccctcaat gagtgggcat cgcggcgcaa cagccctgaa gacttttggg aaacaggtaa
     1501 ccgacgtagt cgcgtggagg gaacgctaac gcagtcgttg gggagagatt atggcaattt
     1561 atacctgaca ttaagccggc aacaatactg gcataccgat gatgtcgaac gattaatgca
     1621 atttggctac agcagtagct ggaagcgtct ctcgtggaac gtctcctgga gttattccaa
     1681 tactgccaga caggggacgg ggaacaacca tgccagtgat aacaccagtg agcagatcta
     1741 catgctctct ttatctgttc ctttatcggg ctggtggggt aatagttacg ccacctattc
     1801 tgtttcgcaa aacgataatt ccggtagctc acatcaactc ggactcagcg gtacggcgct
     1861 ggaaagaaat aacctttcat ggaatttaat gcagtcctat aacagtcatg atgatgaggt
     1921 tggcggtaat atgtccctga cctatgatgg ctcttatggc acggtgaacg gcagctataa
     1981 ctacagccaa aattcccaga ggctgaatta tggtatcaga gggggaattc tggcacacag
     2041 cgaaggggta acgttaagtc aggagttagg tgaaactatt gctcttgtta aagcacctgg
     2101 ggccgccagg ttagaaatag ataatatgcg cggtgctgcg acggactggc gcggctatac
     2161 ggtcaagaca cagctaaacc cttatgatga aaatcgggta gcaatcagcg ataactattt
     2221 ctcgaagtcg aatatagaac ttgataatac cgtcgttacg atggttccca cgcgtggtgc
     2281 agtggttaaa gcggagtttg tgactcatgt gggttatcgc gttctcttca gagtgttaaa
     2341 tgcaaatggt aaaccggtac cttttggagc cattgctgcg atacaagatg caagtttggc
     2401 agattcagga attgtcggtg accgtggcga actttatctt tctggtctac cagaaaaagg
     2461 acaggttacg ttatcctggg gagaaaacgc ctcaacaaaa tgcatcttca attattcatt
     2521 ttcgacacca gaaagtgaga gcggattaat tgaacagggt gtgacatgtc attaaaagga
     2581 tttttatgaa caagtatatc aaacagtggt gctttgctgt gtttatgctc tcgttaagta
     2641 gtgtagcact tgcagctcct aaaggtatct gtacccctga taatggcgta tttcacagta
     2701 cgcttgattt ctcg
//
LOCUS       DAZSNM010000332         2714 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZSNM010000332 DAZSNM010000000
VERSION     DAZSNM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.335"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..173
                     /locus_tag="ACHW42_004139"
                     /pseudo
     CDS             1..173
                     /locus_tag="ACHW42_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(489..827)
                     /locus_tag="ACHW42_004140"
     CDS             complement(489..827)
                     /locus_tag="ACHW42_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945710.1"
                     /translation="MVAETMFPNIAGSRSCIDCHHVAGRTFSKSARRIRGAPLLRRTS
                     RYDAQTSAFGISNDFRCISYVIPFWVVSSAMNVIPIPSLQTHYRSFITTTNGSAPVSR
                     YVPETGSHVP"
     gene            749..1876
                     /gene="ltrA"
                     /locus_tag="ACHW42_004141"
     CDS             749..1876
                     /gene="ltrA"
                     /locus_tag="ACHW42_004141"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR8945711.1"
                     /translation="MFDQQRDGNLYKIWNRLCSGTWFPPPVLEKRIPKSNGKERILGI
                     PTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDALKQCAIRCWRYSWILEVD
                     ISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSCENGELITRTRGTPQGGV
                     ISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRMSDATRLKNRLSERFSEV
                     GLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNFNGERYRKCMPGASNAAM
                     RKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRL
                     LKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE"
     gene            1959..2138
                     /locus_tag="ACHW42_004142"
                     /pseudo
     CDS             1959..2138
                     /locus_tag="ACHW42_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2165..2650)
                     /locus_tag="ACHW42_004143"
     CDS             complement(2165..2650)
                     /locus_tag="ACHW42_004143"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR8945712.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAAELRQ
                     K"
BASE COUNT          713 a          609 c          741 g          651 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtagagg tgggccctcc ggtcagggtt tacaggagca
      241 cagaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtgggttcg ggaagctgaa cgaaagtaaa ctgtccgatg
      361 aagcatcgaa atcttagatg atgccaaaat ggggccgtac agccgtcccc tgataagcgc
      421 agaagacacc tgcttactga ctgcgcggca tccggtgtga aggcagcagg atcccgggcc
      481 agaccatctc agggaacgtg agaacctgtt tccggcacgt atcgggaaac aggggcggac
      541 ccattcgtag tagtgatgaa agatctgtaa tgggtctgga gcgaagggat ggggatcaca
      601 ttcattgcag aagacacaac ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt
      661 gaaataccaa aagcactggt ctgggcatca tatctggatg tgcgtcgcaa caagggagcc
      721 ccccggatgc gacgggcaga ctttgaaaat gttcgaccag caacgtgatg gcaatctata
      781 caagatctgg aaccggctat gttcgggaac atggtttccg ccaccagtgc tggaaaaacg
      841 gatcccgaag tcgaatggca aggaacgcat tctggggatc ccaacagtat ccgatcgaat
      901 tgctcaggga gcgataaaac ttttcatgga agaaaagctt gacccgattt tccatgctga
      961 ttcatatggt taccgaccag gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg
     1021 gtgctggcgt tatagctgga tactggaagt ggacatcagt gcgtttttcg accatgtgag
     1081 acatgatctg gtgctcaaag ctctggaaca ccacgggatg cctaaatggg tcatcctgta
     1141 ctgtcgacgc tggatggaag cgccaatgca gagttgtgaa aatggagaat taataacccg
     1201 aacacgaggc actccgcagg gcggggtcat tagtccgcta cttgctaact tgttccttca
     1261 ctatgcattt gatttgtgga tggaaagaga atatcggggg gtaccgtttg agaggtacgc
     1321 tgacgatatt gtagtacatt gttcacgaat gagtgatgca acaaggctaa agaacaggtt
     1381 atcggagcgc ttctcggaag tcgggctggt cctgaatgca gggaaaacga atatcgccta
     1441 cattgacacg tttaaaagga gaaacgtcgc aacgagtttc acctttctcg gatatgactt
     1501 caaagtgcgt acgctgaaga atttcaacgg cgaacggtac cgaaaatgca tgccgggtgc
     1561 gtcaaatgca gcaatgcgca aaataacaga aacaatcaag aagtggcgta tacatcgctc
     1621 aacagctgag agtttgctgg attttgcgag gcgctacaat gcgatagtga gaggctggat
     1681 cgagtactac ggaaagttct ggtccagaaa tttcaactat cgactgtgga gtgcaatgca
     1741 gtcacgtctg ctcaagtgga tgcagtctaa atacagactt tcgaaccgga aggctcagcg
     1801 aaagctgacg ctggtaagga aggagtatcc gaagctattt gttcactggt atttactacg
     1861 tgcatcaaat gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg gttccgtgag
     1921 cagccgggga gtgaaattct ctccggctga ctcgactcgc gggttccgac agcgatgacg
     1981 aacatgcggc ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggaaccag
     2041 aaaaatggct gcgttacgtc attgagcata tccaggactg gccggcaaac cgggtacgtg
     2101 atctgttgcc ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag
     2161 ccgcttactt ttggcgtaat tctgctgcgc ctttactgaa cacaatgccg tattcagcaa
     2221 gaagtccacg aatgtgattt atcagttgtg tcctttccat tgaaaccaac cgacgcatat
     2281 tcaaaaaagt ctggacgtcc tgttgctcca gagtttttat tggtacgggt cgtatagttc
     2341 catgttggca ggcctcagct attgcctgtg cgtcgttata atcatttttc tggcaccgct
     2401 gataagcttt aacatgttgt gccggaagca atattacatc atggtcgagt ttcctgattt
     2461 cccgggccca gtaatgagat gccccgccag cctccatcgc aacagtcatt ttaggctgtt
     2521 gcaccagcca gttaagcagt tgattgcgag aaagtttggt ctttcctgcg ggtttgccat
     2581 gagcattgat actgaaaaga taaaagttcg ttttagccag atcaatgccg gtgattttct
     2641 gttcagacat ggtatcccgc ttacttttgt tgttaaagtt tatccatcat ggcgcaattg
     2701 aaggctgccg tgaa
//
LOCUS       DAZSNM010000333         2696 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZSNM010000333 DAZSNM010000000
VERSION     DAZSNM010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.336"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(50..625)
                     /gene="dolP"
                     /locus_tag="ACHW42_004144"
     CDS             complement(50..625)
                     /gene="dolP"
                     /locus_tag="ACHW42_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR8945713.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(635..1225)
                     /gene="diaA"
                     /locus_tag="ACHW42_004145"
     CDS             complement(635..1225)
                     /gene="diaA"
                     /locus_tag="ACHW42_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR8945714.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            complement(1245..1640)
                     /locus_tag="ACHW42_004146"
     CDS             complement(1245..1640)
                     /locus_tag="ACHW42_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR8945715.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            complement(1598..>2696)
                     /gene="lpoA"
                     /locus_tag="ACHW42_004147"
     CDS             complement(1598..>2696)
                     /gene="lpoA"
                     /locus_tag="ACHW42_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR8945716.1"
                     /translation="SVASPAQASVSDLTGDQPAAQPVPVSAPATSTAAVSAPANPSAE
                     LKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEELLKSNTPLNVLALNQPENIEN
                     RVNICYFALSPEDEARDAARHIRDQGKQAPLVLIPRSALGDRVANAFAQEWQKLGGGT
                     VLQQKFGSTSELRAGVNGGSGIALTGSPITPRATTDSGMTTNNPTLQTTPTDDQFTNN
                     GGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATLYASSRSAQGTAGPDFRLEMEGLQ
                     YSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDAWSLANHFSQMRQVQGFEING
                     NTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
BASE COUNT          586 a          758 c          693 g          659 t
ORIGIN      
        1 aggcctgata agacgcggca agcgtcgcat caggcattac aaggggctgc tatttaataa
       61 acgtaaacgc tgtggtaacc cgctttacgc cgctcacccg gctggcaata tctgccgccg
      121 ctttcgcttc acgttcagtc accagcccca tcaggaacac ttcaccgttt tcggtggtca
      181 ctttcacgtt ggacgatttc accaggtcgc tggttaagag ctgcgaacgc actttggtgg
      241 tgatccacgt atcgttagat gcttcgccca gaccaatcgg ctggccctga cgaatctcgt
      301 tatacacttc gttggcaccg tctacgccca tagcaatctg tttggcgcga gccgaaagtt
      361 cagcatttgg tgactgccca accagcagca ctttgccctg ataggccgtg acattaatac
      421 gggtttcttt cttaatctgt tcgtctttcg acaatgcgct gttcacgcgc acttccaggg
      481 taccatcgtc cacctgggtg ccgacactgc gtgggtcagt tgcggctttg gtacccacag
      541 cagcggtacc cactacggca gcggcaacac aaccttgcaa cagcagcgcg gaaataagga
      601 ctgcgattgg cgataatgcc ttcatgtatt ctccttaatc atcctggtga gggaaaagcg
      661 tgttatcgat cagatcgcac aggcaattta ccgtcagcat atgcatttcc tgaatgcgag
      721 cactacgatg cgaaggaatg cggatctcca catcctgtgg ccctaacaaa cctgcaagtt
      781 cgccgccgtc atagccggtc aatgccacaa tggtcatatc acgcgtaacg gcggcttcaa
      841 ctgctttaac aatatcgcgg ctgttgccac gggtggaaat ggctaacaat acatctcccg
      901 catgacccag cgcccgcacc tgttttgcat acacttcatc atgtaagcga tcgttggcaa
      961 tcgccgttaa gacaacatta tcagtattta gtgcaatggc aggtaagctg ggccgctccg
     1021 tttcgaaacg gttgatcatg ctggcagcaa aatgctgtgc attggcagcg gaagttccat
     1081 taccacaaca gaggattttg ttgccattga gcagagactg aaccagcgtc atggctgcac
     1141 gggagatggc atccggaagc gcctctgccg ccgcaatttg agtttgaatg ctttcagtga
     1201 agcaagcttt aattctttct tgcacgctaa tccttaaacc ttaattatga gtggtcatta
     1261 aaggcatcct taatccactc aacctcattc ccggtgaagg ctaccacatc gaaccggcaa
     1321 tccacagtat caaaactccc attatgacgc gcgagccaca agcgggcagt ctgtaataat
     1381 ttgtgttgtt tgctgcgggt cacactggct gccgcgccgc cataaagcgc agagcggcga
     1441 tagcgtacct cgacaaaaac ggtggtcagg ccttcacgca ttatcagatc gatctcgccg
     1501 ccacgctcgt tcacgttagc ggcgataaac cgcagtcctt tgccttccag ccagcgacgc
     1561 gcttgtgctt cccacgcatc gccggtctgt ttggtggtta actggcgggg actacctgac
     1621 cttgttggta ctgtagccat gataacttcc tgttaatcac gcaatccggg ttagccgtca
     1681 gacttccggt attaccgttg atttcaaaac cctgaacctg gcgcatttgt gagaaatgat
     1741 ttgccaacga ccaggcatca acgcccatcg catacatgcg agccagcgaa taatcgttgt
     1801 tcaccgcgct gagcgcctgc tgcattaacg gcagattacc gcctgccagc atcgggattt
     1861 cgctgtactg caagccttcc atctccagtc ggaaatccgg gcctgcggtg ccttgtgcgc
     1921 tgcgggaact ggcgtacagc gttgcaccgc tctggctacc gttacgcatg gcgatcatcg
     1981 gtttgataaa agcgatttca cccggcgttg ccacaatgta gaccgcatcg acacgaccgc
     2041 cattattggt gaactggtca tcggttggcg tggtttgcag cgttggattg ttggtcgtca
     2101 tgccggagtc ggttgtcgct ctgggagtaa tcgggctacc cgttaaagca ataccagaac
     2161 cgccgttaac acccgcgcgt aattcgctgg tggaaccaaa tttttgttgc agaacggtgc
     2221 cgccgcccag tttctgccac tcttgcgcaa acgcattggc tacgcgatcg cccaatgcac
     2281 tgcgtgggat cagcaccagc ggcgcttgtt taccctggtc acgaatatga cgcgctgcat
     2341 cgcgcgcttc gtcttccggt gaaagcgcga agtaacaaat attgacgcgg ttttcgatat
     2401 tctccggctg gttcagtgcc agtacgttca gcggggtgtt gctcttcagc aactcttcaa
     2461 cgttattttt cagcaacgga ccaaccacaa tactcgcgcc atcctgctga acctggctta
     2521 agatctggct aagtggttgt gatgaggtat cgtagatttt cagctctgcg gatggatttg
     2581 cgggtgcgct taccgctgcg gtgcttgtcg ccggggcgct tacaggcacc ggctgggccg
     2641 caggctgatc accggtcaga tcgctaaccg aggcttgtgc cgggctggca acgcta
//
LOCUS       DAZSNM010000334         2693 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZSNM010000334 DAZSNM010000000
VERSION     DAZSNM010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.337"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..237)
                     /locus_tag="ACHW42_004148"
     CDS             complement(<1..237)
                     /locus_tag="ACHW42_004148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945717.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVF"
     gene            complement(383..982)
                     /locus_tag="ACHW42_004149"
     CDS             complement(383..982)
                     /locus_tag="ACHW42_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945718.1"
                     /translation="MSLNILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(1152..2090)
                     /locus_tag="ACHW42_004150"
                     /pseudo
     CDS             complement(1152..2090)
                     /locus_tag="ACHW42_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(2153..2527)
                     /locus_tag="ACHW42_004151"
     CDS             complement(2153..2527)
                     /locus_tag="ACHW42_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFR8945719.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(2531..>2693)
                     /locus_tag="ACHW42_004152"
     CDS             complement(2531..>2693)
                     /locus_tag="ACHW42_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HFR8945720.1"
                     /translation="LMMSLILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQI
                     SNNLTSGAK"
BASE COUNT         1035 a          513 c          384 g          761 t
ORIGIN      
        1 aaagacagac agtatagcat aaaaaacaac atcactgcca actttattaa ttgcttgttg
       61 caaatctaat gtggcaggac ttataccatg tcgagcttgt tctgctgttt gaacttgtag
      121 atcaatttca ttccataggt agtgaccaaa tatatttcct ccagcaggac taaaaggatc
      181 accagagatc caaaaactac ggacatacca ataaatgcca ccaagaattg ttgccaagct
      241 aaaaataaga aatgtctgta tgtttctctt tttcgctatc aatataacaa atatgatggc
      301 tccatatatg gcaccaaata gttttacgcc acaagcaatt ccaataaaaa aaccacataa
      361 aaaaattaat tcattttgtg ttttattgtt ttctgagcga atatatataa gaatactggc
      421 tgcagcaaaa caaagatagc gttaaatcaa tatatgcata accaagatga tctttaagtg
      481 gattgatagt aaatagtagg attgtagcaa taatgcctgg ataataagtt ttcgtaattt
      541 cccatgaaca gccaacaagt ccacaggcta ttacaaaaaa acagatattt gctaaaaact
      601 gagctaacct tacatctcct aactgtaaac ctaaagccat taaaagatct gcattttgcg
      661 ggaacagagg aaaacgcaaa tattgttcaa caacaattgc atggtgctca actaatgaac
      721 gagccaaagg taagtgatac atagtatcat cccaatcacc gggagctcgc agggacgata
      781 acaatgtaac cacaaaaatc aaaacaagaa ataaaataac aaacagcgta ctgaactcaa
      841 tattatttat tgccagccat ctaaattgct gacgtttaat aatgcttaaa acccaacaca
      901 ccgtaaaaat taaaaagata gcaatcttat taaattgccc gaccatgccc agaaagtaaa
      961 ttatcaaaat atttaaagac atccctatgc ataggcaata aaaaataaag aaaatttgtt
     1021 aagtcctgcc acccctcctt aaaaaaagat gacaatacaa tattaccaat gacaaaaaat
     1081 gtcattccaa aagcaaaaca atacacaaca tacaaataca acggcaattc aatcatagta
     1141 aaatactcta tttacattat cagtttatag tttttttcag aaagataaaa taatgccttc
     1201 atttcacgtc tagttgttgt atttgaatgc aaaataatgc cacatacaaa tgccattatt
     1261 gacgataaca tgatggaggc agttaaaaag gcagtcggaa atcttctcac caaacctgta
     1321 tcaatataat caacaatgat gggcaatcct aaaattatag acaaagcagc aagtaaaacg
     1381 ccgataatac taaaaaagta tagtggccgt tcaagcgagt aaagtttaat tattgttttc
     1441 agaatcttga acccatcact ccaagttgat agtttgctca cactcccttc agatcgctcg
     1501 ccatattttg tattaacctc accatacttc atacgtaatt ctaaagcatg aattgtaagc
     1561 tcagtttcga tttcaaatcc ccttgataga catggaaaag atttaacata gcgtctggaa
     1621 aatacacgat accccgacaa catatcagta aaattccctt taaagatagt ctgaacagta
     1681 ccagtcaaca atttatttcc tcagcgatgc ccttttctgt atgtattttg atctccattc
     1741 tcttgacgac atcctacaac catatccaat tgatttttta tgagatggtt aatcatttct
     1801 ggagcagaac ttgcatcata agtgtcatct ccatcaacca ttagataaat atccgcatct
     1861 acatctgaga acatacgtct tacgacattc cccttgcctt tcagaggaac agagtgaact
     1921 gttgctccag catcctctgc cacttgtgct gtactgtcag tcgaattatt atcaaataca
     1981 tatatagaag cagttggaat attcgttttg aaatcattta tgacttttgc tattgccttc
     2041 tgctcattat aacaaggaat aattacagcg atattaaaag agtgatacat aagcccctca
     2101 tataaactat ttagctaatg tgcaagtaat tgtgttttta acaaatcgtt tactaacgaa
     2161 aaatgaaata ttttgatgcg caatatccta aaaccaaact aaaaagagaa aaggtgaaca
     2221 atgtaaatat tggagataaa gctaagagat caaataaaac gccgaataaa taactcatcg
     2281 cccccataaa aaatataaat ataaaatatc tagccttggt tgcagatact ttaaatgagc
     2341 atcttgcatt tgcaaaaaaa gaaaaggttg ctgcacatat aaaaccaaca atgttacttc
     2401 ggccttgacc aaaagctaaa ttattataac acgcataaaa taccccccaa tgtaaagcag
     2461 tattgacaat tccaattaaa aaataactaa aaaaagaact ggtcaataac ttccctattt
     2521 ttaacatcct ttattttgct ccagaagtga ggttattact aatttggata ttttctatag
     2581 aaaataccca taaaactaca acgcctataa taaaaattcc catgggtgta aaaagaaatg
     2641 gattagttat agaaacggta atactgaaaa aaagtattaa cgacatcatt aat
//
LOCUS       DAZSNM010000335         2671 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZSNM010000335 DAZSNM010000000
VERSION     DAZSNM010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.338"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            149..1315
                     /gene="nhaA"
                     /locus_tag="ACHW42_004153"
     CDS             149..1315
                     /gene="nhaA"
                     /locus_tag="ACHW42_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR8945721.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1381..2280
                     /gene="nhaR"
                     /locus_tag="ACHW42_004154"
     CDS             1381..2280
                     /gene="nhaR"
                     /locus_tag="ACHW42_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR8945722.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2318..>2671)
                     /locus_tag="ACHW42_004155"
     CDS             complement(2318..>2671)
                     /locus_tag="ACHW42_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR8945723.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          620 a          589 c          685 g          777 t
ORIGIN      
        1 gtcaaaaacg catctcaccg ctgatggcgc aaattcttca atggctcgta aaaaacgaat
       61 tattcctaca ctataatctg attttaacga tgattcgtgc ggggtaaaat agtaaaaacg
      121 atctattcac ctgaaagaga aataaaaagt gaaacatctg catcgattct ttagcagtga
      181 tgcctcggga ggcattattc ttatcattgc cgctatcctg gcgatgatta tggccaacag
      241 cggcgcaacc agtggatggt atcacgactt tctggagacg ccggttcagc tccgggttgg
      301 ttcactcgaa atcaacaaaa acatgctgtt atggataaat gatgcgctga tggcggtatt
      361 tttcctgtta gtcggtctgg aagttaaacg tgaactgatg caaggatcgc tagccagctt
      421 acgccaggcc gcatttccag ttatcgccgc tattggtggg atgattgtgc cggcattact
      481 ctatctggct tttaactatg ccgatccgat tacccgcgaa gggtgggcga tcccggcggc
      541 tactgacatt gcttttgcac ttggtgtact ggcgctgttg ggaagtcgtg ttccgttagt
      601 gctgaagatc tttttgatgg ctctggctat tatcgacgat cttggggcca tcattatcat
      661 cgcattgttc tacactaatg acttatcgat ggcctccctt ggcgtcgcgg ctgtagcaat
      721 tgcggtactc gcggtattga atctgtgtgg tgtacgccgc acgggcgtct atattcttgt
      781 tggcgtggtg ttgtggactg cggtgttgaa atcgggggtt cacgcaactc tggcgggggt
      841 aattgtcggc ttctttattc ctttgaaaga gaagcatggg cgttctccag cgaagcgact
      901 ggagcatgtg ttgcacccgt gggtggcgta tctgattttg ccgctgtttg catttgctaa
      961 tgctggcgtt tcactgcaag gcgtcacgct ggatggcttg acctccattc tgccattggg
     1021 gatcatcgct ggcttgctga ttggcaaacc gctggggatt agtctgttct gctggttggc
     1081 gctgcgtttg aaactggcgc atctgcctga gggaacgact tatcagcaaa ttatggtggt
     1141 ggggatcctg tgcggtatcg gttttactat gtctatcttt attgccagcc tggcctttgg
     1201 tagcgtagat ccagaactga ttaactgggc gaaactcggt atcctggtcg gttctatctc
     1261 ttcggcggta attggataca gctggttacg cgttcgtttg cgtccatcag tttgacagga
     1321 cggtttaccg gggagccata aacggctccc ttttcattgt tatcagggag agaaatgagc
     1381 atgtctcata tcaattacaa ccacttgtat tacttctggc atgtctataa agaaggttcc
     1441 gtggttggcg cagcggaggc gctttattta actccacaaa ccattaccgg acagattcga
     1501 gcgctggaag agcgcctgca aggcaaatta tttaaacgca agggacgtgg tctcgaaccc
     1561 agcgagctgg gagaactggt ctatcgctat gccgataaaa tgttcacctt aagccaggaa
     1621 atgctggata ttgtgaacta tcgcaaagaa tccaatttat tgtttgacgt tggcgtggct
     1681 gatgcacttt ccaaacgcct ggtcagtagc gtacttaacg ccgcagtggt agaaggcgag
     1741 cccattcatc ttcgctgctt cgaatccacc cacgaaatgc tgctggagca attaagtcag
     1801 cataaactgg atatgatcat ttctgactgt ccgatagact ctacgcagca ggaaggcctg
     1861 ttctccgtga gaattggcga atgtggcgtg agtttctggt gtacaaatcc accaccagaa
     1921 aaaccgttcc cggcttgtct ggaagaacgg cgacttttga ttcctgggcg acgttcaatg
     1981 ttagggcgca aattgcttaa ctggtttaac tcccagggat taaacgtaga aatcctcggc
     2041 gagtttgatg atgccgcttt gatgaaagct tttggtgcga tgcacaatgc aatcttcgtt
     2101 gccccaacgc tttatgcata tgacttttat gccgataaaa ctgtcgtaga aattggtcgc
     2161 gtcgagaatg tgatggaaga gtaccatgct atttttgctg agcggatgat tcagcacccg
     2221 gcggtacagc gaatctgcaa tacggattat tctgcgcttt ttagtccagc ggtgcgttaa
     2281 tcggcagctc ccccaaagtt aaggtggggg agatagatta gttgtacatt accacgattt
     2341 tgactcggct cattatttgc ccgcttgaga cattgtttcc atatgtacgc gggcgaataa
     2401 atagaggaat ctgattactt ccttcatggg gatgctgaaa agagtagtaa ttgcttttac
     2461 catactggat ttcattcgta ccatcattgg agagttgaaa gcctatttga cccgcaccgt
     2521 cctcactgtt ttctaactca ttttttaata tgctggttcc tgactcctgt atcgtcactg
     2581 gttcaaagta atattcaacg tcactgggtt caaaaactaa ttcaccttgc tcgccctgga
     2641 tgcagtcaaa tcttatatga tgaaactgat a
//
LOCUS       DAZSNM010000336         2655 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZSNM010000336 DAZSNM010000000
VERSION     DAZSNM010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.339"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..224
                     /locus_tag="ACHW42_004156"
     CDS             <1..224
                     /locus_tag="ACHW42_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR8945724.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="ACHW42_004157"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="ACHW42_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR8945725.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="ACHW42_004158"
     CDS             518..1108
                     /locus_tag="ACHW42_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR8945726.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /locus_tag="ACHW42_004159"
     CDS             1348..1608
                     /locus_tag="ACHW42_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR8945727.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="ACHW42_004160"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="ACHW42_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR8945728.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..>2655
                     /gene="repA"
                     /locus_tag="ACHW42_004161"
     CDS             1899..>2655
                     /gene="repA"
                     /locus_tag="ACHW42_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR8945729.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTA"
BASE COUNT          711 a          606 c          697 g          641 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgcca
//
LOCUS       DAZSNM010000337         2627 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZSNM010000337 DAZSNM010000000
VERSION     DAZSNM010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.340"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..545)
                     /gene="ygeQ"
                     /locus_tag="ACHW42_004162"
     CDS             complement(<1..545)
                     /gene="ygeQ"
                     /locus_tag="ACHW42_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
                     /protein_id="HFR8945730.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1207..2196)
                     /locus_tag="ACHW42_004163"
     CDS             complement(1207..2196)
                     /locus_tag="ACHW42_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945731.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2280..>2627)
                     /locus_tag="ACHW42_004164"
     CDS             complement(2280..>2627)
                     /locus_tag="ACHW42_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945732.1"
                     /translation="TSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAER
                     IAKELASQKERTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIV
                     ADLESGGNWMDSL"
BASE COUNT          682 a          613 c          631 g          701 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg agttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggagcctacg acaaatcgaa gacaaccagc taacctattg tttcttaaag acaggtgaat
      841 ccagttggtt gtagtgatag tcacacattt agagatagaa ggtcaagact tgaagcacgt
      901 aatattccct ctaaaggaat agaactcacg gtctgactac ccggcccttt tggtctggct
      961 caggtcaggc tatgggggct tttggctcgc tctttatatt agatgaggtc tcacattttt
     1021 cagtcaaaac tcatcgtcta atagcccata gccagaccac cagtcccagc tttagcagga
     1081 agaccacgag atagactttg tgtaggtcca tttggccctc ctactttctg tattcacaaa
     1141 ctagattact agtacaaaat aaaggccaga catccagttc ggaagtctga ccaatagtcg
     1201 ttattgttag ttagcttctt tagtgaagct caggcgtttg actacaggca ggacagcagt
     1261 agccgagcca gtacccatct tgatggtcgt agcagcttca gctacaacaa acaccttgcc
     1321 gagaatctca aggtcgtgag actgggagtc tccatcacgt gaaaggacca cagtaatgct
     1381 ctcgccatcg tgacggaagc aaccgaagct cagagccgta aaacgcttag ccatcatgaa
     1441 cacgccacgg tcttcaccgt tgtaataggc gacgtcagtc cccagcatat cgacgatagc
     1501 gtcttcacca ccgaagccct gcttagcagc cagacgggag agagccttga ggtgtgactc
     1561 atgggcgata atgccgtagt cagaaacctc agaaccgaaa gaagactccg tgttctcgta
     1621 cagggcatcc atgatagcgt cagccttagc ggtcaggcta ccagtcaggg ccggaagttc
     1681 tacggtcttc agcagctttg aggactccag cacgtcaacc atcttagagt taatggtacg
     1741 aatcacgtta tcgcagatgt tcagtgcgtc ctgctggagc gtcccctgag tgaaaccagt
     1801 ggtcgacttc tggtcccaaa gctttgtttg gtgagacacg aggatcgcct gttcaatcgg
     1861 ctcaacggtg atttcagtgc cagacggagc ggtcacaact tcaggtcgct taccaccacg
     1921 gacagggacc agcacagagc ggtcagccag agtgtgagca atgattggag tcggtaagct
     1981 gttacgggtc ggcaggactt ctacagggaa cgggatgtta tcttgagcgt ctttaagtac
     2041 accgttcagc atagccttaa cagtcttagc taactggagg gcatagacct gagggtcggc
     2101 gttagtcggc agaccttcat tgacctgaat gttgtcggta tcggtgatgc gtactggctc
     2161 aatgccaaac tggaaagcgt ttttagtcat attcattata attaatctcc ttgagtcaaa
     2221 tacttgaagt ccctaacagg cagtcaggga ccgtgtgttc tcagatgagg gacgcttgct
     2281 tacaagctgt ccatccagtt accgcctgac tcaaggtcag cgacgatagc ggcacgtgag
     2341 cgaccagcgg aatgagtagc gctgcggagg ttttcaccat aggtccggcg ttcagctttg
     2401 acctgttcgc ggcgctgtag ctctttttgc cattcgtctt cggtcatgaa catttcgcgg
     2461 gtacgctctt tctgagacgc tagctcttta gcgatacgct cagcttcagc tcgtttgttc
     2521 atctggcggc gttggtaggc ggtcatcagg tcattactga agaagttacc aatggtcacg
     2581 ccagcttcag ccagagcttc cagtaactcc atcgcctcgc ggctggt
//
LOCUS       DAZSNM010000338         2603 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZSNM010000338 DAZSNM010000000
VERSION     DAZSNM010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.341"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            82..885
                     /gene="agaC"
                     /locus_tag="ACHW42_004165"
     CDS             82..885
                     /gene="agaC"
                     /locus_tag="ACHW42_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFR8945733.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            875..1666
                     /gene="agaD"
                     /locus_tag="ACHW42_004166"
     CDS             875..1666
                     /gene="agaD"
                     /locus_tag="ACHW42_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFR8945734.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1667..2422
                     /locus_tag="ACHW42_004167"
     CDS             1667..2422
                     /locus_tag="ACHW42_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFR8945735.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          638 a          613 c          642 g          710 t
ORIGIN      
        1 tcaggacgtc cctggcgatc aaaaagaaca aatccctgac taaatctaaa atcgccttaa
       61 tattggtttg aggtaataaa aatgcatgaa ataaccctac ttcagggatt atccctagcg
      121 gcgttagttt tcgttctggg gattgatttt tggctggaag ccttattttt attccgcccg
      181 ataatcgttt gtaccctaac tggcgctatt ctcggtgata ttcagactgg cttaattacc
      241 ggtggtctga cagagttggc tttcgccgga ttaacccctg caggtggtgt tcagccgccc
      301 aacccgatta tggcaggtct gatgaccacc gtcattgcat ggtctacggg cgttgatgcc
      361 aaaacagcaa ttggtcttgg cctgccgttt agtttgttaa tgcagtacgt cattctgttc
      421 ttctattccg ctttctcatt atttatgacc aaagccgata aatgcgcgaa agaggcggat
      481 acggcagcgt tttcccggct taactggaca acgatgctca tcgtcgcttc agcgtatgcg
      541 gtgattgctt tcctctgtac ttacctggca caaggggcga tgcaggcgct ggtgaaagcg
      601 atgcccgcct ggttgaccca cggctttgaa gtggctggcg gtattctgcc tgccgttggt
      661 tttggcttgc tgctgcgcgt aatgttcaaa gcgcaatata tcccttacct gatcgccggt
      721 ttcctgtttg tttgctacat ccgggtcagc aacctgttgc cggttgccgt actgggcgca
      781 ggctttgcgg tgtatgagtt tttcaatgcg aaatcccggc agcaagcgca accgcagccc
      841 gttgccagta aaaatgaaga agaggactac agcaatggga tctgaaatca gtaaaaaaga
      901 tatcacccgt ctgggctttc gttcgtcgct gctgcaagcg agctttaact acgaaaggat
      961 gcaggcgggc ggttttacct gggcgatgtt gccgatcctg aaaaagattt ataaggacga
     1021 caaaccgggc ttaagcgcgg cgatgaaaga taacctcgaa tttattaata cccacccgaa
     1081 tctggtcgga ttcctgatgg ggttattaat ttcgatggaa gaaaaaggag aaaaccgcga
     1141 caccattaaa ggcctcaaag tggcactgtt tggcccaatc gccgggattg gcgatgcgat
     1201 tttctggttt actttgttgc cgattatggc gggaatttgc tcatcatttg ccagccaggg
     1261 aaacctgctg gggccgattc tatttttcgc cgtttacctg cttatctttt tcctgcgcgt
     1321 cggctggacc cacgtcggtt attcagtcgg cgtgaaggcg atcgataaag tgcgagagaa
     1381 ctcgcagatg attgctcgct cggcaaccat cctcgggatc acggtaatcg gcgggctgat
     1441 cgcttcgtat gtgcatatta acgtggtgac atcgtttgcc atcgacagta cccacagcgt
     1501 cgcgctgcaa caggatttct tcgataaagt cttcccgaac attttaccga tggcctacac
     1561 cctgctgatg tattacttcc tgcgggtgaa aaaagcgcat ccggtgctgt taatcggcgt
     1621 gacttttgtg ctctctattg tttgttccgc attcggcatt ttgtaaatgg aacgaggcac
     1681 tgcttctggc ggtgcctctt tacttaagga atttcatcct gtgcaaaccc ttcagcaagt
     1741 tgaaaactat acggcgttaa gtgaacgagc cagcgaatat ttattggctg tgatccgtag
     1801 caaaccggat gccgtgattt gcctggcgac cggagccacg ccattactga cgtatcatta
     1861 tctggtagaa aaaatccacc agcagcaggt tgatatcagc cagctcacct tcgtgaagct
     1921 cgacgaatgg gtggatctgc cattaacgat gcctggcacc tgcgaaactt tcctacaaca
     1981 gcatatcgtg cagccgctgg ggctacgtga agaccagctc atcagctttc gctccgaaga
     2041 gataaatgag acagagtgcg aacgggtaac gaacctgatt gcgcgcaaag gcggtctgga
     2101 tttatgcgtt ctcggattgg ggaaaaacgg tcatcttggg ctgaacgaac cgggagaaag
     2161 cctacaaccg gcctgccata tcagtcaact tgatgccaga acacagcaac atgagatgtt
     2221 aaaaaccacg ggtcgccccg tgactcgtgg gatcacctta ggcctgaagg atattctcaa
     2281 tgcccgcgag gttttgttat tggtgactgg cgaaggaaag caggatgcga cagaacgttt
     2341 tctcacggct aaagtctcca ccgctatccc ggcttcattt ttatggctgc acagtaactt
     2401 tatttgttta attaatacct aaccagatga aaaatttgct caaaattttt aaattaactt
     2461 attcaacagt cacgcattat attaaataac atttgactgg gttgaacata acgccaatag
     2521 caaaaggagt tatgttcaac aatttaattt tagatataca tataacttgt tctattaata
     2581 aatcacctcg tattctgact cgg
//
LOCUS       DAZSNM010000339         2585 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZSNM010000339 DAZSNM010000000
VERSION     DAZSNM010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.342"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            94..1209
                     /gene="adrA"
                     /locus_tag="ACHW42_004168"
                     /gene_synonym="dgcC"
     CDS             94..1209
                     /gene="adrA"
                     /locus_tag="ACHW42_004168"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR8945736.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1226..2035)
                     /gene="proC"
                     /locus_tag="ACHW42_004169"
     CDS             complement(1226..2035)
                     /gene="proC"
                     /locus_tag="ACHW42_004169"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR8945737.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2155..>2585
                     /locus_tag="ACHW42_004170"
     CDS             2155..>2585
                     /locus_tag="ACHW42_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR8945738.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKW"
BASE COUNT          570 a          660 c          729 g          626 t
ORIGIN      
        1 agtgctaacg ggtgagctac gaaagtggct cacccgaaat ttcatacttc tgcctttagc
       61 cccgtctcta taatttggga aaattgtttc tgaatgttcc caaaaataat gaatgatgaa
      121 aactttttca aaaaagcggc ggcgcatggg gaggaacctc ctttaactcc tcaaaacgaa
      181 catcagcggt ccgggctgcg cttcgcccgt cgcgtcagac taccccgtgc ggttggcctg
      241 gctggcatgt tcttaccgat tgcttcaacg ctggtttcgc acccgccgcc gggctggtgg
      301 tggctggtgt tggtcggctg ggcgttcgtc tggccgcatt tagcctggca gatagcgagc
      361 agggccgtcg atccgcttag ccgggaaatt tacaacttaa aaaccgatgc agtattagcg
      421 ggaatgtggg taggcgtaat gggcgtaaac gtgctgcctt ccaccgctat gttgatgatt
      481 ctgtgtctga atttgatggg ggcaggcggc ccccgtctgt ttgtcgcggg tctggtgttg
      541 atggtggttt cctgccttgt caccctcgag cttacgggca ttaccgtgtc gttcaatagt
      601 gctccgctgg aatggtggct ctcccttccc attattgtcg tttatcctct gatgtttggc
      661 tgggtcagct accagacggc aaccaaactg gcggaacata aacgcaggtt gcaggtcatg
      721 agtactcgcg acggcatgac gggcgtgtat aaccgacgtc attgggaaac tatgttacgc
      781 aatgaatttg ataactgtcg gcggcataat cgcgatgcaa cgttactgat tatcgatatc
      841 gaccatttca agagcatcaa cgatacctgg ggtcatgatg tgggcgatga agcgattgtg
      901 gcgcttaccc gacagttaca aattaccctg cgcggtagcg atgtgattgg tcggtttggc
      961 ggcgatgagt ttgcagtaat catgtccggt acgccagctg agagcgccat taccgccatg
     1021 ttacgggtgc gtgaagggct aaacacgtta cgcttgccga atacgccaca ggtaacttta
     1081 cggattagtg tgggggttgc gccgctgaac ccacaaatga gtcactatcg tgagtggttg
     1141 aaatcggcag atttggcgct ttacaaagca aagaaagccg gacgtaaccg caccgaagtg
     1201 gcggcctgac gtccggcgaa agtcatcagg atttgctgag tttttctgat ttttccatac
     1261 acttcgtcat cgcttcgatc actgcggcac ggaagccttt ctcttccagt acgcgtaccg
     1321 cttcaatggt ggtgcctccc ggtgagcaga ccatatcttt cagtgccccc ggatgttctc
     1381 ccgtttccag caccattttt gcggaaccca ttaccgcctg agcggcaaat ttatacgcct
     1441 gggcgcgtgg catcccgccc agcacggcgg cgtcggccat cgcttcgata aacataaata
     1501 cgtaggctgg cgaagaaccg ctcacaccga ccaccgggtg gatcatcggc tcagcaatta
     1561 cttccgcttc gccaaagcag cggaaaatat tcagcacatc agcggtatct tctggggtta
     1621 ccagcgcgtt tggcgttacg gaggtcatcc cggcattaac cagcgcggga gtgttcggca
     1681 tggcgcggat aattttccgg tcatggccca gcgcgcgggc aagctggtcg agcgtgacac
     1741 ctgcagcaat agaaacgacc agagagtctt tattcaggct ggaggtgatt tcgctaagca
     1801 ctttaatcat gatgccaggt ttaacggcag caaaaatgat gtcggcgatt tgcgccactt
     1861 cttgcgccga ttctgcggcg ttgatgccga actggtcatg cagggcggcg actttatccg
     1921 gggagggggt gtatacccag atttgccctg gaagcacctg accgctggca atcagaccgc
     1981 cgagaatggc ttttcccata ttgccgcagc caataaaacc gattttcttt tccattgcct
     2041 cactcctgcc gtgaaattca ttgttttgat aatcgctggc agaagcataa acagaactat
     2101 gccggaaggc aaaagcgcga cacaatagag gattacctaa caaaggatga ctttatgaca
     2161 atttgggtgg atgccgacgc gtgtcccaat gtaattaaag agattttgta tcgcgcggcg
     2221 gaacgtatgc agatgccgct ggtactggta gcaaaccaga gtttacgcgt gccgccatcg
     2281 cgatttattc gtacgctgcg cgtcgcggca ggtttcgacg ttgccgataa cgaaattgtc
     2341 cggcagtgtg aagcgggcga tttggtgatc accgcagata tacctttggc tgctgaagcc
     2401 atcgagaaag gcgcagcggc gcttaattcg cgcggcgaac gttacacgcc agcgaccatt
     2461 cgtgagcgcc tgacgatgcg cgattttatg gataccttac gtgccagtgg gatccagacc
     2521 ggcggaccag atagcctttc acaacgtgac cgccaggcct ttgccgcgga gctggagaag
     2581 tggtg
//
LOCUS       DAZSNM010000340         2551 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZSNM010000340 DAZSNM010000000
VERSION     DAZSNM010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.343"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..776
                     /locus_tag="ACHW42_004171"
     CDS             join(<1..274,274..776)
                     /locus_tag="ACHW42_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
                     /protein_id="HFR8945739.1"
                     /translation="TPRAGRPWAEHRKIINGMFWVLCSSAPWRDLPERYGAWKTVYNR
                     FNRWSKSGVINIIFNRLLSLLDANGFIDWSATALDGSNIRALKCAAGGSKKHPDIDGD
                     NGPGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAHESQFAQRLLDGIGVQRQNGSMKR
                     RGHAVLADKAYSGRALRNELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVER
                     CFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            complement(813..944)
                     /locus_tag="ACHW42_004172"
     CDS             complement(813..944)
                     /locus_tag="ACHW42_004172"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945740.1"
                     /translation="MARSAKSRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFD"
     gene            1148..1219
                     /locus_tag="ACHW42_004173"
                     /pseudo
     CDS             1148..1219
                     /locus_tag="ACHW42_004173"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1362..1583
                     /locus_tag="ACHW42_004174"
                     /pseudo
     CDS             1362..1583
                     /locus_tag="ACHW42_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1604..1666)
                     /locus_tag="ACHW42_004175"
                     /pseudo
     CDS             complement(1604..1666)
                     /locus_tag="ACHW42_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            2116..>2551
                     /gene="ospC1"
                     /locus_tag="ACHW42_004176"
     CDS             2116..>2551
                     /gene="ospC1"
                     /locus_tag="ACHW42_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HFR8945741.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLS"
BASE COUNT          789 a          552 c          557 g          653 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt attcagggcg cgcattgcgc aacgagctga
      541 aaaataacgg tataaaggca gtaatccccc gaaagtcaaa tgagaaaatg gcatcggatg
      601 gacgtgcaca gcttgatcgt gatgcgtact gcaaccgtaa tgtcgttgaa cggtgctttg
      661 ggcggctgaa agaatatcgc cgcatcgcca cgcgttacga caaaacggcg agaaattacc
      721 tggcgatggt gaaactgggc tgcatccgac tcttttatca acgcttacgt aattaaggga
      781 cacagcctag tctccaggat tcccggggcg gttcaatcaa agcgcagaac ttctgcgtct
      841 tcttcgtcaa aaaaatcatc ttcgtgaagc ttcacgacat agcggggaag tttgcttctt
      901 tgagaggcgg gtttacgttt acgggattta gctgaacggg ccatataacc acctgaaaga
      961 caatgacatt gcctgttttt ataacggtaa ttgcagacca tgacaaaccg cagccgtcag
     1021 gctgcctact cgagtcacaa ggctctttgt gtccgatatg ccggcagcgc attacccatg
     1081 aaactggctg gaacgtacac cataaggtga agaaagttat gggggcggtg aagaattatc
     1141 taaccttgtc ttgttacatc caaattgcca tcgacagtta cacagttgtg aagccggctc
     1201 tctcaaaaag ggcttataaa ggcttgagcg gtgtaccggg aaaccggtat gcaccgttct
     1261 taggggaggg cagtccagca atgaactgtc cttacccgac aaacatccaa aacgaacgga
     1321 acgtgctgga atccgcatat aacccgttgt aaagtgagga aatggacacc tcacttgctc
     1381 atgagaacgc ccgcctgcgg gcactgttgc agacgcaaca ggacactatc caccagatgg
     1441 ctgaatacaa ccgcctgctc tcacagcgga tggcggctta tgcttccgaa atcaaccgac
     1501 tgaaggcgct ggttgcgaaa ctgcagcgta tgcagttcgg taaaagctca gaaaaacttc
     1561 gcgcaaaaac cgaacggcag attccatttt cacgggcgat ctacgccaca caggctctgg
     1621 gttgcagtga ctgctccatt aaggcgattt tgaactcata agggaagtaa caaaaaaact
     1681 gcaaaagtac aataattata tatggccaaa ctaacctcta ttaaaaaaca tcagtttttc
     1741 acacttagtt gagcttcatg aaaaaatcat cgaggatttc attgcagaat acacattcca
     1801 ctaactggaa gcactccata agaggaatca gagaaagtat cgttttttta tagtaaaatt
     1861 cattgctgtt caataaaatt gatatagtta ttaaattatg aaaataattt cacaaaaaag
     1921 attaacttga gttcttaagc atcggatttt agatattttg aaatatctgg catcacataa
     1981 catcaaaagt atataatact cttatctaat tacatgcagt atattgtata tatactcaaa
     2041 aattgtaatt cataccctgc tatatcttgt agggaaagat attaattaaa actgttttca
     2101 tataaggttc attttatgaa tatatcagaa acactgaact cagcaaatac ccaatgcaat
     2161 atagattcta tggataacag attacataca ttgtttccaa aagtgacatc agtgcgaaac
     2221 gctgcacaac aaactatgcc agatgaaaaa aatttaaaag atagtgcaaa tattattaaa
     2281 agtttcttta ggaaaactat agcagcacag agttatagta gaatgttctc tcaaggctct
     2341 aactttaaat ctttaaatat agcaattgat gcaccatcag acgctaaagc ctcatttaag
     2401 gctattgagc accttgacag attatcgaag cattatatat ctgaaataag ggaaaaactt
     2461 catcctcttt ctgcagagga actcaatttg ctttcgctaa ttattaattc tgatttaatc
     2521 ttcagacatc aaagtaattc tgatttgtct g
//
LOCUS       DAZSNM010000341         2537 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZSNM010000341 DAZSNM010000000
VERSION     DAZSNM010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.344"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..953
                     /gene="adeQ"
                     /locus_tag="ACHW42_004177"
                     /gene_synonym="yicO"
     CDS             <1..953
                     /gene="adeQ"
                     /locus_tag="ACHW42_004177"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR8945742.1"
                     /translation="TLFRIRYWMISNIPLSLRIGITSGIGLFIALMGLKNTGVIVANK
                     DTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGV
                     YSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDSNGK
                     FPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMF
                     FSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEG
                     IALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1006..1458
                     /locus_tag="ACHW42_004178"
     CDS             1006..1458
                     /locus_tag="ACHW42_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR8945743.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1669..>2537
                     /gene="nepI"
                     /locus_tag="ACHW42_004179"
     CDS             1669..>2537
                     /gene="nepI"
                     /locus_tag="ACHW42_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFR8945744.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPL"
BASE COUNT          485 a          583 c          687 g          782 t
ORIGIN      
        1 tcacgctgtt tcgtatccgg tactggatga tctccaacat tccattaagt ttacgtattg
       61 gtatcaccag tggaatcgga ttatttattg ccttaatggg attaaaaaac actggtgtta
      121 ttgtcgccaa taaagacacg ctggtgatga ttggcgattt aagttctcac ggcgtgttgt
      181 taggtatttt agggtttttt attataaccg tgttgtcatc acgtcatttt catgccgcgg
      241 tgctggtttc tattgtggtg acgtcttgct gtggattatt tttcggtgat gttcatttta
      301 gcggcgtcta ttccattccg cctgatatta gcggcgtcat tggtgaagta gatttgagcg
      361 gcgcgttaac acttgaactc gccggtatca ttttctcctt tatgctgatc aacctatttg
      421 attcatcagg aacattaatt ggtgtaactg ataaagcggg cttaatagat agcaacggta
      481 aattccccaa tatgaataag gcgctgtatg ttgatagcgt cagttcggtg gcaggcgcat
      541 ttatcggtac ctcgtctgtt actgcctata ttgaaagtac ttctggtgtg gcagtcggtg
      601 gtcgcacggg gctaacggct gtcgtggtcg gcgttatgtt cctgttagtt atgttcttct
      661 caccgctggt ggcgatggtt cctccttacg caaccgctgg agcgttaatc tttgttggcg
      721 tgctgatgac ttcgagcctg gcgcgcgtta actgggatga ttttaccgaa tcggtgcctg
      781 cgtttattac cacggtgatg atgcccttta ctttctcgat caccgaaggg attgcactcg
      841 gctttatgtc gtactgcatc atgaaagtgt gcaccgggcg ctggcgcgat ctgaacctgt
      901 gtgtggtggt ggtcgcagct ctgttcgcat tgaagattat tctggtggat tagtaactgg
      961 cgagggtttc cagatatcat gagttctgat tacgcaggag aactcatgat ctggataatg
     1021 ctcgccacgc tggcggtagt gtttgtggtt ggttttcggg tgctgacatc cggggccaga
     1081 aaagcgattc gccgtctcag cgatcggctg aatatcgatg tcgtacccgt ggagtcgatg
     1141 gtcgatcaaa tgggaaagtc agccggtgac gaatttttac gttatttgca tcgcccggat
     1201 gagtcgcacc tgcaaaacgc cgcgcaggtg ttgcttatct ggcaaattgt cattgtcgat
     1261 gatagcgaac agaacctgct gcaatggcac cggattttac aaaaagcccg cctttccgcg
     1321 ccgattaccg atgctcaggt caggctggcg ctgggttttc tgcgtgaaac cgaacctgaa
     1381 atgcaggata ttaatgcttt tcagatgcgc tataacgcgt tctttcagcc tgccgagggc
     1441 gttcactggt tgcattgaaa ccgtttcgtc ttatcagcct acagaccgcg cctgacctgt
     1501 cgggtggata agacgttcac gccgcatccg gtaatctttg ccctgttccg tttcgatctt
     1561 aactccagct caagcaacac cgtaaaagtt gcaaaaacgt taaattcgtc acacatttca
     1621 atgttaaagt ggtcggcttt tcccctgaaa catgccacgg gtaacaccat gagtgaattt
     1681 attgccgaaa accgcggcgc ggatgccatc acccgaccga actggtcagc cgttttctcg
     1741 gtggcgtttt gtgtcgcctg tctgattatc gttgagtttt tgcccgtcag tttgttgacg
     1801 ccaatggccc aggatttagg catttcggaa ggggttgccg ggcaatcggt gaccgtgacc
     1861 gcctttgtgg caatgtttgc cagtttgttt attacccaga caattcaggc cactgaccgc
     1921 cgctacgttg ttattttgtt tgccgttttg ctgacgctct cctgcttgct ggtttccttt
     1981 gctaactcat tcagtttgct tttaatcggt cgtgcctgtc tggggctggc gctgggcggg
     2041 ttctgggcga tgtcggcgtc gctgaccatg cgtctggtac cgccgcgtac ggtgccgaag
     2101 gcgctgtcgg tgatcttcgg cgcggtttct attgcgctgg tgattgccgc gccgttgggc
     2161 agttttttag gcgagcttat cggttggcgc aatgtcttta atgcggcggc ggtgatgggc
     2221 gtgctgtgta ttttctggat tatcaaatca ttgccttcac tgccaggcga accctcgcat
     2281 cagaaacaaa atactttccg cttattacaa cgtccgggtg tgatggcagg gatgatcgcc
     2341 atcttcatgt ctttcgccgg gcagtttgct ttcttcacgt atattcgccc ggtgtatatg
     2401 aacctggcgg gattcggcgt ggatggctta acgctggtgc tgttgagttt tggtatcgcc
     2461 agctttattg gtacgtcgct ttcgtcgttc attcttaaac gttcggtaaa actggcctta
     2521 gcaggcgcgc cgttaat
//
LOCUS       DAZSNM010000342         2536 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZSNM010000342 DAZSNM010000000
VERSION     DAZSNM010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.345"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(214..333)
                     /locus_tag="ACHW42_004180"
                     /pseudo
     CDS             complement(214..333)
                     /locus_tag="ACHW42_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            577..949
                     /locus_tag="ACHW42_004181"
                     /pseudo
     CDS             577..949
                     /locus_tag="ACHW42_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1511..2458)
                     /gene="icsP"
                     /locus_tag="ACHW42_004182"
     CDS             complement(1511..2458)
                     /gene="icsP"
                     /locus_tag="ACHW42_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFR8945745.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
BASE COUNT          732 a          545 c          517 g          742 t
ORIGIN      
        1 tagtaaaggg tgttatattt tcatatagta tgcaagcttc tttgtatact atgatttaaa
       61 aaaacggaac aaaaatgcaa ttatattctg gcattgttta atgcggtttg tcttttttat
      121 tcattatttc aatgaaagaa tttcaactac aaagttaatt gatcctacct acataatata
      181 gacacagtac ctaaagtacc gtggagatca tccggggaag ccccgggtct gataccagtg
      241 caggtagacc gctatcttgt tccggtaatg ttctgccgtt ctttacggca tcacctctag
      301 cagaaacatc gcgtaactgc gccaagtatg atgaacttct cttaccgctg cgatctggtt
      361 caacaatatg cagatggtac acaacctctt taaccagccc atccggatta ttcagagccg
      421 cttccgcaat ccggaataaa agtgctgttt taccgtgaac cctctgaaaa tcagcgagca
      481 gtgttttaac cagccgacgt tcagagcgta ctgacagacg gtggacaatt tgaataaaca
      541 gttcaatcag accgtagatg ctctcccgtt ggcgacatgc tgggagcggt ggaacaccgc
      601 ttcggcaacg agcttccggc gtctccagtg aagtgactga cggataatgg ttcatgctac
      661 cgagctaatg aaacacgcca gttcacccgg atgttgggac tttgttgaac cgaagaacac
      721 ggcggtgcgg agtccggaga gtaacagaat agcaaagagc ttcgtgaaaa caataaagtg
      781 tgactacatc agtatcatgc ccaaacctga cgggttaacg gcagcaaaga accttgcaga
      841 ggcattcgag cattataacg aatggcatcc gcatagtgcg ctggattatc gctcgccacg
      901 ggaatatctg cggcagcggg ccaatgataa caggtgtctg gaaatatagg ggcaaagcca
      961 cccacgaacg atccagcata taatcaagaa attagatttc atttaacata agatagatta
     1021 tgtacaccaa gaggcgtttc cttaatttct gaatttacag gctcttttgc tctctttgct
     1081 atgctgtgga tttggaaaga tagagtgctg gctgattatt taggtatttt catcagcatg
     1141 tccctgatag cactgttcca tcagcgcttt caggcgataa tagtagacct tgggtagcct
     1201 ctgcccatgt gtaacttgat accccgagaa cagccacgat gatctaagcg gttcttattt
     1261 ctccggtctc agggtcggta atgccaacag taagtccaca gtaatcaaca aagagttttt
     1321 tgaaccgccc cggaaatcct ggagactaaa cgccctgaga aagaggtaaa cagaatgact
     1381 aaaaatacac gtttttcccc cgaagtccgt caacgggcag ttcgtatggt tctggaaagt
     1441 cagggcgaat atgactcaca atgggcggca atttttagcc atgaaaacgt aatcaactcg
     1501 gtgaaccaat tcaaaaaata tactttatac ctgcggaagt gagaaaacca atatattcaa
     1561 tacttgcact atttttaatg gtgccagata tattggtact ttgctcataa agagatgtat
     1621 cacctttttt attagaaatg taatttctgg agccttctat ataaaatttt gcactggggg
     1681 tgacatagta tccaatattc gcagcaacag agcagtaatt ttggtttttt atttcatctt
     1741 taaaaattgt ctcagtctga tagtgtttat ctgtatcaga tgaaagaacc caaccactat
     1801 atttcagttc tgctccaaac tcaaaattct catggcggta attagcagtt aatccaatat
     1861 aaggtatttt gaagagctgt ttgtaaccta ttgttctttc accactagga tgtgcccctt
     1921 ttttattcct gctccccccg ttttcactat aaatataact ccctcccatt gcattaaaac
     1981 tgtaacgact ctcttggtaa cctgctatta gtccaagtcg ataatccaaa ttatttaata
     2041 accaaccttt aagattcagc tcgaattcgt tagcatcacg gagatgcgta ttgggatgcc
     2101 agctctggtc ggtccatacc tgaggggtat tggaattgtt ccagtcttta tcaaccatgc
     2161 ttgctttctg gttacctaaa gtagtccaac cggaaactcc gaaagacact tttggaatta
     2221 gcttccaatc gatgccacct ctaacaatag tcgcattact gtatttccag tccagttgac
     2281 taatttttct ccccccttcc ttgggatgat aaacgcgttc ttttgttttg ccactcagag
     2341 atcctagact aatatccgta ctgatgttgt ccggtatgaa aagtggatag ttagtggtag
     2401 catgtgtagt aaagatcgca ggtacacaaa gtgcaagtac aaagaatttt aatttcatcg
     2461 agaactctac ttttttggtt gaaatgtcca tgatcttctt actttataag ataaacgtca
     2521 atgccaagca ttattt
//
LOCUS       DAZSNM010000343         2506 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZSNM010000343 DAZSNM010000000
VERSION     DAZSNM010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.346"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            158..493
                     /gene="eutS"
                     /locus_tag="ACHW42_004183"
     CDS             158..493
                     /gene="eutS"
                     /locus_tag="ACHW42_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR8945746.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            506..985
                     /gene="eutP"
                     /locus_tag="ACHW42_004184"
     CDS             506..985
                     /gene="eutP"
                     /locus_tag="ACHW42_004184"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR8945747.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            960..2333
                     /gene="eutA"
                     /locus_tag="ACHW42_004185"
     CDS             960..2333
                     /gene="eutA"
                     /locus_tag="ACHW42_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR8945748.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
BASE COUNT          575 a          633 c          710 g          588 t
ORIGIN      
        1 atattcatgc gtcgcatatg aaagtttatt cataacagta aatatctctc cattcttatt
       61 gatctacctc accttttaaa cgcgcttgcc gaattttgtt atttactctg acgaaaaatt
      121 gtcacgatac acgaaagttt ttcacaggcg gcgactcatg gataaagaac gcatcattca
      181 ggaatttgtg cccggcaaac aggtcacact ggcgcatctc atcgcgcatc ccggtgagga
      241 actggcgaag aaaatcggcg ttccggatgc gggcgcaatt ggcattatga cgctaactcc
      301 cggcgaaacg gcgatgatcg ccggtgattt agcgctgaaa gccgctgatg tgcatatcgg
      361 ttttctcgac cgcttcagcg gtgcattggt gatttatggc tcggtgggcg cagtagaaga
      421 agcgttatca caaaccgtca gcggtttagg tcgcttatta aattatacgc tttgtgaaat
      481 gaccaaaagt taatacgagg cattcatgaa acgaattgcg tttgtgggta gcgtcggggc
      541 aggaaaaaca acgttattta atgcgttgca ggggaattat accctcgcca gaaaaacact
      601 ggccgtggaa tttaatgata agggcgatat tgatacgccg ggtgaatatt ttagccatcc
      661 ccgctggtat cacgccttaa ttaccacgct acaggatgta gacatgctga tctatgtcca
      721 tggcgcaaat gacccggaaa gtcggttgcc tgccgggttg ctggatattg gcgtcagcaa
      781 acggcaaatc gccgtcatca gtaaaacgga catgccagat gctgacgtcg ccgcaacgcg
      841 aaagttattg cttgagacgg gtttcgaaga gccgattttc gagctaaaca gccacgaccc
      901 gcaaagcgtg cagcaactgg tggattatct ggcatctctc accaaacagg aggaagcagg
      961 tgaaaaaact catcacagcg aatgatattc gtgaagcaca cgcgcgcggc gaacaggcaa
     1021 tgtccgtggt tctgcgcgcc agcattatta ccccggaagc ccgtgaagtg gcggatctac
     1081 tgggttttac catcaccgaa tgtgatgaat cgatcccggt aacggcgtcc gtgcccgcca
     1141 gcgtgccagc cgacaaaacc gaaagccagc gcatccgcga aaccatcatt gcccagctgc
     1201 cggaaggcca gtttaccgaa agcctggtcg cacagctgat ggaaaaagtg atgaaggaaa
     1261 aacagtcgct gggcgatttt gtcgtcgcca gcgccgggcc gcacctcgaa tccgtgatcg
     1321 ccggtcacgg agccggggcg caaacccttt ctgaacaacg gctgtgtcgg gtactgaata
     1381 tcgatatcgg cggtggcacc gcgaactacg ccctgttcga tgccggaaaa atcagcggca
     1441 ctgcctgtct caacgtcggt ggtcgcctgc tggaaaccga cagccagggg cgcgtggttt
     1501 acgctcataa accggggcag atgattgtgg atgagtgctt cggtgcaggc actgacgccc
     1561 gttcgctgac cggcgcgcag ctggtgcagg ttacccggcg gatggcggcg ctgattgtcg
     1621 aagtgattga cggaacgctt tcgccgctcg cgcaggcatt gatgcaaacc ggtttgctgc
     1681 ccgcaggtgt tacgcccgaa atcattacgc tttctggagg cgtgggcgaa tgttatcgcc
     1741 accagcccgc caacccgttc tgttttgccg atattggccc acttctggca acggcgctgc
     1801 atgaccatcc gcgcctgcgt gagatgaatg tgcagtttcc ggcgcaaacc gtacgctcca
     1861 cggtgattgg cgcgggtgca catacccttt cgctctctgg cagcacaatc tggctggagg
     1921 gcgtacaact gccgctgcgc aatttgccgg tggcgatccc gattgatgaa acggatctgg
     1981 tgagtgcctg gcaacaggcg ctgcttcagc tggatcttga tcccaaaact gacgtgtacg
     2041 tgctggcgct tcccgcctcg ctgcctgtgc gttacgccgc ggttctgacg gtcatcaacg
     2101 cgctggtcga tttcgtcgcg cgttttccga atccgcatcc cctgctggtg gtggccgggc
     2161 aggactttgg taaagctctg ggcatgttgt tgcgcccaca gctacaacaa ctcccgttgg
     2221 cagtcattga cgaagtgatt gtccgcgcgg gggactatat cgacattggt acgcctcttt
     2281 ttggcggatc ggttgtgccg gtgacggtga aatcactcgc atttccttcc tgaatacggt
     2341 agttatcagg tatggtgctt ggtgtctggg tgttagtggg attatatccg gcagtgtatc
     2401 cggttaaggt gattggtctg gcaagatgat tggatgcttt tgtggtgaaa aatgttttgg
     2461 tgttttagca taaccctgaa gaaagaataa aaataaaaag gaaaaa
//
LOCUS       DAZSNM010000344         2495 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZSNM010000344 DAZSNM010000000
VERSION     DAZSNM010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.347"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1038
                     /locus_tag="ACHW42_004186"
     CDS             <1..1038
                     /locus_tag="ACHW42_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR8945749.1"
                     /translation="VLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRNLPSGVVP
                     AVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHR
                     YGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELS
                     WWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPL
                     PHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCMTCGNQA
                     DDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLLIRFLMH
                     ARNSGVLKWKA"
     gene            1039..1404
                     /locus_tag="ACHW42_004187"
     CDS             1039..1404
                     /locus_tag="ACHW42_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR8945750.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            1401..2089
                     /locus_tag="ACHW42_004188"
                     /pseudo
     CDS             1401..2089
                     /locus_tag="ACHW42_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
     gene            2283..2358
                     /locus_tag="ACHW42_004189"
     tRNA            2283..2358
                     /locus_tag="ACHW42_004189"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2316..2318,aa:Ile,seq:cat)
     gene            2360..2436
                     /locus_tag="ACHW42_004190"
     tRNA            2360..2436
                     /locus_tag="ACHW42_004190"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2394..2396,aa:Lys,seq:ttt)
BASE COUNT          653 a          572 c          693 g          577 t
ORIGIN      
        1 gttctggttc cggaactcgg gctggtggtc cttaagcccg gtcgtgaatc catgccggta
       61 ttccacaata cccgggtact ggtggagccg gaaccgaaaa gcatgcgtaa tctgccgtcc
      121 ggggtcgttc ctgccgttcg ccagccgcta gtggaagaca aaacattgct gccgtttttc
      181 agtaacgcac gggtgattcg tgctgctggt ggtgctggcg cattgtctga ctggctgttg
      241 cgccatatta aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgtcatt
      301 caccgttatg gtaccggcgc aatggtattg tgctggcact gcgacaacca gctgcgtgac
      361 cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg gatgattgac
      421 gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc tgaattatcc
      481 tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg
      541 ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat cgtgccggga
      601 gagcagaccg ccaccagcat actgaagcag cgcacaaaaa atcttgcgcc gctgcctcac
      661 gcccaccagc aacagaatcc accacaggaa gagacggtgg tcagcattgc cgttgatccg
      721 gaatctccgg ctcagtatct ccagcgccag aaaccacaac gggaagagat gcctgtatac
      781 acgcgctggg taaaaacgca gaaatgcatg acgtgtggca atcaggcaga tgatccgcac
      841 cacatcattg gtcatggact gggagggatg ggaacaaagg ctgacgattt gtttgttatt
      901 ccgctgtgtc gtaaatgcca taacgaacta cacgccgggg taaaagattt tgaagaaaaa
      961 cacggtagtc agttgttgtt gctgattcgt tttttaatgc acgcgagaaa ttcgggtgtt
     1021 ctgaagtgga aagcataaat gaccgaacgc atagaatttg ttttgcctta cccgccaacg
     1081 gtgaacacct actggcgacg tcgtggcagc acatattttg tatcaaaagc cggtgagcgt
     1141 tatcgccgtg atgtggcgct aattgttcgc cagcagcgac tgaaattaaa cctgtccgga
     1201 aggctggcga taaagattat tgcagagcca ccggataagc gccgtcgtga cctggacaat
     1261 atcctgaagg caccgctgga tgcgctgacg catgctggac tacttataga cgacgagcag
     1321 tttgatgaaa tcaatattgt gcgcggtcag ctcgttcctg gtgggcggct ggggataaaa
     1381 atcacagaac tggggtgcgc atgaataacc agtatttaca gtttgtgcgt gagcagctca
     1441 ttatcgccac cgctgatttg agtggggcaa caaaaggtca gcttgaagcc tggcaagaga
     1501 atgccatgtt cgatacaggg cgttacaggc gtaaaaaaat ccggtaccgc gatgaagtga
     1561 ctggaaaaat gataacgcgg gataatccac caatcccggg aaagcaatcg ctggcgaagg
     1621 ggacgtcaat tcctctggtc agtccggttg agttttcgac atcatcgtgg cggcgggctg
     1681 ttctgtctct tgaagagcac tataaagcct ggttgttgtg gtgttacagc gggagtattt
     1741 gttgggaata tcagatcgcg ataacacagt gggcgtggaa tgaatttaat gctcaatccg
     1801 gtaccagaaa aattgcaggg aaaacgcagg aacgcctgaa aaaattaatc tggctggcgg
     1861 cgcaggcagt aaaagcagaa ctttttggtg gggaaggtta tgaataccag gagctggcat
     1921 tactggcggg agtgacaact aaaaactggt ccaaaacatt tactcgtcac tgggttgcaa
     1981 tgaaacacat ttttcaccga ctggatagtg aggctttatt gtttgtaatg agaacacgtt
     2041 caaaaaaaag acggcatttt caaagcaaag tgttgcaaaa gtagattgaa aggcatatat
     2101 tcatgcaaat ctgatacttt gccgattttg tacgtgatgg caaaagcaaa caaaacccgc
     2161 ccacaagcgg gttttttgtg ccacttatct cggatagaca tggtgaatgc gctggtggag
     2221 gagataaggg tgatttttga atgcttgcaa cattgatttc gtaacgttat tatcctgcgc
     2281 ccggcccttt agctcagtgg tgagagcgag cgactcataa tcgccaggtc gctggttcaa
     2341 atccagcaag ggccaccaac cgccactagc tcatcaggaa agagcgtcaa ccctttaagt
     2401 tgagtgtgcg aggttcgagt ccccggtggc ggtccagtgc cgacttggct cagtaggtag
     2461 agcaactgac ttgtaatcag taggtcacca gttcg
//
LOCUS       DAZSNM010000345         2491 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZSNM010000345 DAZSNM010000000
VERSION     DAZSNM010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.348"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>2491
                     /locus_tag="ACHW42_004191"
     CDS             <1..>2491
                     /locus_tag="ACHW42_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR8945751.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQEQ"
BASE COUNT          730 a          578 c          602 g          581 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggagcaa t
//
LOCUS       DAZSNM010000346         2491 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZSNM010000346 DAZSNM010000000
VERSION     DAZSNM010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.349"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..330
                     /locus_tag="ACHW42_004192"
     CDS             <1..330
                     /locus_tag="ACHW42_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945752.1"
                     /translation="LAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQAVREI
                     AGQERDRTATSEREAALPESVLRESQREREAVREVARENLLQERLQQMERDMVRDLQK
                     EKTLGGD"
     gene            350..1096
                     /gene="traX"
                     /locus_tag="ACHW42_004193"
     CDS             350..1096
                     /gene="traX"
                     /locus_tag="ACHW42_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR8945753.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            1151..1711
                     /locus_tag="ACHW42_004194"
     CDS             1151..1711
                     /locus_tag="ACHW42_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR8945754.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGETPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            1843..2055
                     /locus_tag="ACHW42_004195"
     CDS             1843..2055
                     /locus_tag="ACHW42_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR8945755.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
     gene            2301..>2491
                     /locus_tag="ACHW42_004196"
     CDS             2301..>2491
                     /locus_tag="ACHW42_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR8945756.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWST"
BASE COUNT          612 a          569 c          781 g          529 t
ORIGIN      
        1 ctggcacagc ggcaggctga agaggccatc cgccgtgaaa cggaacgtcg cgcagatgaa
       61 attgtccgta aaatggcaga gaacaaacct gacctgccgg acggcaaaac agagcaggct
      121 gtcagggaga ttgccggaca ggagcgtgac cggactgcca cttctgaacg ggaagccgcg
      181 ctgccggaga gtgtgctgcg tgaatcacaa cgggagcggg aagcggtccg ggaggttgcc
      241 cgggaaaatc tgctgcagga gcgtctgcag cagatggagc gggatatggt tcgtgacctg
      301 cagaaagaga aaaccctggg cggagactga tacaggaaga taaacgctga tgacaacgga
      361 taacacgaac acgacacgta acgattcact ggctgcacgg accgatacct ggttgcagtc
      421 atttctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac tggtgctgat
      481 ggtactggac cacatcaatc tgatattcca gctgaagcag gaatggatgt ttctggccgg
      541 gcgaggagcc tttccgctgt ttgccctggt gtgggggctg aatctgtccc gtcatgcgca
      601 tatccggcaa ccggccatta accggctgtg gggatggggg attattgccc agttcgcata
      661 ttatctggca ggctttccct ggtatgaggg gaatatcctg tttgcctttg cagtggcggc
      721 acaggtgctg acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg ccattcttct
      781 gatggccctg tgggggcctt tgtccggcac cagttacggc attgccgggc tgctgatgct
      841 ggcagtcagc caccggctgt accgggcgga agacagagcg gaacgtctgg cgctggtcgc
      901 ctgtctgctg gctgttattc cggcgcttaa ccttgccacc agtgatgcag cggcggtggc
      961 tggtcttgtg atgacggtgc tgaccgttgg tctggtgtcg tgtgcaggaa aatcattacc
     1021 ccgtttctgg cccggggatt ttttcccggt attctacgcc tgtcatctgg ctgtgctggg
     1081 cgttctggcg ctgtgacggg tgtggtatct ttgaccgtaa caggatgatt cgtcagaggc
     1141 agaacacagc atgacagagc agaagcgacc ggtactgaca ctgaagcgaa aaacggaagg
     1201 ggagacgcct gtccgcaggc ggaaaaccat catcaatgtc accacgccac caaagtggaa
     1261 ggtgaaaaag cagaagctgg ccgagaaagc agcccgggaa gcggagctgg cggcaaaaaa
     1321 agcgcaggcc agacaggcgc tgtccattta tctgaacctg ccctcgctgg atgaggccgt
     1381 gaacaccctg aaaccctggt ggccgggatt atttgacggt gaaacgcccc ggcttctggc
     1441 ctgcggtatc cgggacgtgt tactggaaga cgtggcgcag cggaatatcc cgctctcgca
     1501 taaaaaactg cgcagggcgc tgaaggccat cacccgttca gaaagctatc tgtgtgccat
     1561 gaaagccggt gcctgccggt atgatacgga agggtatgtg acggagcata tttctcagga
     1621 ggaggaagcg tatgcggcag cgcgtctgga taaaatccgc cgccagaaca ggataaaggc
     1681 agagcttcag gccgtgcttg atgagaaata aaaaaaagcc tccatcagag aggaggcagg
     1741 gaaataaggc ttaaaaggaa ataacttccg tacccgaaag gaagccaatt cagtagatta
     1801 tttaacacca taatactgac gggttaaaga ggagtgataa agatgaatgg attcagaaac
     1861 agttcacgga acggtcaggt ctggcgctgg cagcgtgccg gaagccgggc cgttattctg
     1921 gaggtcagtg gacgctggat ggaagcggca gaagcatggc gacgggctgc cggtgttgcg
     1981 ccccggacag actggcagca gtttgcccga aaaagggctg aacactgcca ccggcgcagc
     2041 cggggaaggg tgtaacgaaa aaagcagcct tcattactcc agggattcag aaggctgaat
     2101 aatgcatgag atttgctttt tttaatatcc ggtcgggcag tatgaccgga cagttctgag
     2161 tagcacagag ttttttccgt attaagtcgt gattttatat tttgtgatta atttcacaaa
     2221 ataaggtgtt attcagtgtg tgctgcgata ttcaggatta cctgaaatac aggtgccatt
     2281 tatctgatat ggagaataac atgaggaaat atattccact ggtattattt atcttttcat
     2341 ggccggtatt gtgtgcagat attcatggac gggttgttcg tgtactggac ggtgatacca
     2401 tcgaggtcat ggactcacgg aaggcagtgc ggatcaggct ggtaaatatt gatgcaccgg
     2461 aaaagaaaca ggattatggc cggtggtcaa c
//
LOCUS       DAZSNM010000347         2489 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZSNM010000347 DAZSNM010000000
VERSION     DAZSNM010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.350"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1079
                     /gene="serC"
                     /locus_tag="ACHW42_004197"
     CDS             <1..1079
                     /gene="serC"
                     /locus_tag="ACHW42_004197"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR8945757.1"
                     /translation="FNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGKEFIQ
                     VAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYWAASA
                     IKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAIDETPD
                     FGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKANIAC
                     PSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELLYGVI
                     DNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGMRASI
                     YNAMPLEGVKALTDFMVEFERRHG"
     gene            1150..2433
                     /gene="aroA"
                     /locus_tag="ACHW42_004198"
     CDS             1150..2433
                     /gene="aroA"
                     /locus_tag="ACHW42_004198"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR8945758.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT          604 a          587 c          662 g          636 t
ORIGIN      
        1 tcttcaattt tagttctggt ccggcaatgc taccggcaga ggtgcttaaa caggctcaac
       61 aggaactgcg cgactggaac ggtcttggta cgtcggtgat ggaagtgagt caccgtggca
      121 aagagttcat tcaggttgca gaggaagccg agaaggattt tcgcgatctt cttaatgtcc
      181 cctccaacta caaggtatta ttctgccatg gcggtggtcg cggtcagttt gctgcggtac
      241 cgctgaatat tctcggtgat aaaaccaccg cagattatgt tgatgccggt tactgggcgg
      301 caagtgccat taaagaagcg aaaaaatact gcacgcctaa tgtctttgac gccaaagtga
      361 ctgttgatgg tctgcgcgcg gttaagccaa tgcgtgaatg gcaactctct gataatgcgg
      421 cttatatgca ttactgcccg aatgaaacca tcgacggtat cgccatcgac gagacgccag
      481 acttcggcaa agatgtggtg gtcgccgccg acttctcttc aaccattctt tcccgtccga
      541 ttgacgtcag ccgttatggt gtaatttacg ctggcgcgca gaaaaatatc ggcccggctg
      601 gcctgacaat cgtcatcgtt cgtgaagatt tgctgggcaa agcgaatatc gcgtgtccgt
      661 cgattctgga ttattccatc ctcaacgata acggctccat gtttaacacg ccgccgacat
      721 ttgcctggta tctatctggt ctggtcttta aatggctgaa agcgaacggc ggtgtagctg
      781 aaatggataa aatcaatcag caaaaagcag aactgctata tggggtgatt gataacagcg
      841 atttctaccg caatgacgtg gcgaaagcta accgttcgcg gatgaacgtg ccgttccagt
      901 tggcggacag tgcgcttgac aaattgttcc ttgaagagtc ttttgctgca ggccttcatg
      961 cactgaaagg acaccgtgtg gtcggcggaa tgcgcgcgtc tatttataat gccatgccgc
     1021 tggaaggcgt taaagcgctg acagacttca tggttgagtt cgaacgccgt cacggttaat
     1081 gccgaaattt tgcttaatcc ccacagccag cctgtggggt ttttatttct gttgtagaga
     1141 gttgagttca tggaatccct gacgttacaa cccatcgctc gtgtcgatgg cactattaat
     1201 ctgcccggtt ccaagagcgt ttctaaccgc gctttattgc ttgcggcatt agcacacggc
     1261 aaaacagtat taaccaatct gctggatagc gacgacgtgc gccatatgct gaatgcatta
     1321 acagcgttag ggttaagcta tacgctttca gccgatcgta cgcgttgcga aattatcggt
     1381 aacggcggtc cattacacgc agaaggtgcc ctggagttgt tcctcggtaa cgccggaacg
     1441 gcaatgcgtc cgctggcggc agctctttgt ctggatagca atgatattgt gctgaccggt
     1501 gagccgcgta tgaaagagcg cccgattggt catctggtgg atgcgctgcg cctgggcggg
     1561 gcgaagatca cttacctgga acaagaaaat tatccgccgt tgcgtttaca gggcggcttt
     1621 actggcggca acgttgacgt tgatggctcc gtttccagcc aattcctcac cgcactgtta
     1681 atgactgcgc ctcttgcgcc ggaagatacg gtgattcgta ttaaaggcga tctggtttct
     1741 aaaccttata tcgacatcac actcaatctg atgaagacgt ttggtgttga aattgaaaat
     1801 cagcactatc aacaatttgt cgtaaaaggc gggcagtctt atcagtctcc gggtacttat
     1861 ttggtcgaag gcgatgcatc ttcggcttct tacttcctgg cagcagcagc aatcaaaggc
     1921 ggcactgtaa aagtgaccgg tattggacgt aacagtatgc agggtgatat tcgctttgct
     1981 gatgtgctgg aaaaaatggg cgcgaccatt tgctggggcg atgattatat ttcctgcacg
     2041 cgtggtgaac tgaacgctat tgatatggat atgaaccata ttcctgatgc ggcgatgacc
     2101 attgccacgg cggcgttatt tgcaaaaggc accaccacgc tgcgcaatat ctataactgg
     2161 cgtgttaaag agaccgatcg cctgtttgcg atggcaacag aactgcgtaa agtcggcgcg
     2221 gaagtggaag aggggcacga ttacattcgt atcactcctc cggaaaaact gaactttgcc
     2281 gagatcgcga catacaatga tcaccggatg gcgatgtgtt tctcgctggt ggcgttgtca
     2341 gatacaccag tgacgattct tgatcccaaa tgcacggcca aaacatttcc ggattatttc
     2401 gagcagctgg cgcggattag ccaggcagcc tgaatgaaca acgggcaata aatagccaaa
     2461 tctttcttta tcaaaacgtc ggcacattg
//
LOCUS       DAZSNM010000348         2415 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZSNM010000348 DAZSNM010000000
VERSION     DAZSNM010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.351"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            9..1058
                     /locus_tag="ACHW42_004199"
     CDS             9..1058
                     /locus_tag="ACHW42_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR8945759.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1132..1989
                     /gene="nfo"
                     /locus_tag="ACHW42_004200"
     CDS             1132..1989
                     /gene="nfo"
                     /locus_tag="ACHW42_004200"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR8945760.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            1992..>2415
                     /locus_tag="ACHW42_004201"
     CDS             1992..>2415
                     /locus_tag="ACHW42_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR8945761.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVR"
BASE COUNT          545 a          607 c          650 g          613 t
ORIGIN      
        1 agagggttat gacgaatatc accttacaga aacaacatcg tacactgtgg cattttattc
       61 cggggttagc cctgagtgca gttatcaccg gggtcgccct gtggggtggt tccattcccg
      121 cggttgcggg tgccgggttt agtgccctca ccctcgcaat cttgttgggg atggttttag
      181 gcaacaccat ctatccgcac atctggaaaa gctgtgacgg tggtgtgctg tttgccaagc
      241 aatatttatt acgtctgggt atcattcttt atggcttccg tctgacgttc tcgcaaattg
      301 ccgatgtcgg tatcagtggg atcatcattg acgtgctgac gctgtccagt accttcctgc
      361 tggcttgctt cctggggcag aaagtgtttg gtctggataa gcacaccagt tggttgatcg
      421 gtgccggtag cagtatctgt ggtgctgccg cggtactggc gactgagccg gtagtgaaag
      481 cggaagccag taaagtaacc gtggctgttg caaccgttgt tatcttcggg accgtcgcga
      541 ttttcctcta cccggcgata tatccgctga tgtctcaatg gtttagtccg gaaactttcg
      601 gtatctacat tggttctact gtgcacgaag tggcgcaggt ggtggcggca ggtcatgcca
      661 tcagcccgga tgcagaaaac gcagcagtta tttccaaaat gctgcgcgtg atgatgctgg
      721 ctccgttcct catcctgctg gcggcgcgtg ttaaacagct gtctggggcg aacagcggcg
      781 agaaaagcaa aatcactatt ccgtggtttg ccatcttgtt catcgtagtt gccatcttta
      841 actcgttcca cctgttaccg cagagcgtgg tgaacatgct ggtaacgctg gataccttcc
      901 tgctggcaat ggcgatggcg gcgctgggtc tgaccactca cgtcagcgcg ctgaaaaaag
      961 ctggggcgaa acctctgctg atggcactgg tactgtttgc ctggctgatt gttggtggtg
     1021 gtgcgattaa ctatgtgatt caaagcgtca tcgcataaac cactacttct tgctcctgtt
     1081 aacccgctat cattaccgtt ttcctccagc gggtttaaca ggagtcctcg catgaaatac
     1141 attggagcgc acgttagtgc tgctggcggt ctggcaaatg ccgcaattcg cgccgccgaa
     1201 atcgacgcaa ccgcgtttgc cttgttcacc aaaaaccaac gtcagtggcg tgccgcaccg
     1261 ctcacgacgc aaaccatcga tgaattcaaa gccgcctgtg aaaaatatca ctacacatcg
     1321 gcgcaaattc ttccccacga cagttatctg atcaacctcg gacatccggt cactgaagct
     1381 ctggaaaaat cgcgcgatgc ctttatagat gaaatgcagc gttgcgaaca actggggctt
     1441 tctttgctca acttccaccc tggcagccat ctgatgcaga tttcagaaga ggattgcctt
     1501 gcgcgtattg ccgaatccat caacattgcg ctggataaaa ctcaaggtgt aacagcggtg
     1561 attgaaaaca ccgccggtca gggcagtaac ttagggttta aattcgaaca tctcgcggcg
     1621 attatcgacg gcgtggaaga taaatcccgc gtcggcgtct gcattgatac ctgccatgct
     1681 ttcgctgccg ggtatgattt gcgtactcca gccgaatgcg aaaaaacgtt cgctgatttt
     1741 gcccgtattg tcggctttaa gtatttgcgc gggatgcacc ttaacgatgc gaaaagtacc
     1801 tttggcagcc gcgttgaccg ccatcatagc ctcggtgaag gcaatatcgg tcatgatgcg
     1861 ttccgctgga ttatgcagaa cgaccgtttc gacggcattc cgctgatcct tgaaaccatc
     1921 aacccggata tctgggcaga agagatcgcc tggctgaaag cgcaacaaac tgaaaaagcg
     1981 gtagcctgaa gataaataac cgggaaaagg agatccttgc aattttacgg cgtaacccgc
     2041 tgattcagca gaacgaaatt gcggacatgc tgcaaatcag tcgttcgcgc gttgcggcgc
     2101 atattatgga tttaatgcgc aaaggccgga ttaaagacaa aggttacatt ctcaccgagc
     2161 aggaatactg cgtagtggtg gggacaatca atatggatat tcgcgggatg gcggatatcc
     2221 attacccgca agcggcttct catcccggta caattcattg ttcggcaggc ggcgttggac
     2281 gcaatatcgc ccacaatctg gcgctgttag gccgtgacgt ccatttgctt tcagtgattg
     2341 gcgatgactt ttatggcgaa atgctcctgg aagaaacgcg ccgtgccggc gtgaatgtct
     2401 ccggctgcgt tcgtt
//
LOCUS       DAZSNM010000349         2396 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZSNM010000349 DAZSNM010000000
VERSION     DAZSNM010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.352"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..884
                     /gene="speC"
                     /locus_tag="ACHW42_004202"
     CDS             <1..884
                     /gene="speC"
                     /locus_tag="ACHW42_004202"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR8945762.1"
                     /translation="LGIESRKAILARCKLFRPFIPPVVDGKLWQDYPTSVLASDRRFF
                     SFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVPATILAHYLRENG
                     IVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAEVLPSVYNKYPVR
                     YRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAHSAYIRGEVELVR
                     IRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNLLPGFSPELQGVY
                     SETDADGMKRLYGYVLK"
     gene            complement(933..2189)
                     /gene="nupG"
                     /locus_tag="ACHW42_004203"
     CDS             complement(933..2189)
                     /gene="nupG"
                     /locus_tag="ACHW42_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR8945763.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
BASE COUNT          620 a          560 c          647 g          569 t
ORIGIN      
        1 agttgggtat tgagtcgcgc aaggctattc ttgcgcgctg taagctgttc cgcccgttta
       61 tcccgcccgt tgttgatggc aaattgtggc aggattatcc gacatcagtg ttagccagcg
      121 accgccgttt tttcagtttt gagccggggg cgaagtggca cggctttgaa ggatatgccg
      181 cggatcagta ttttgttgat ccgtgcaagc tgttactcac tacaccaggt atcaatgccg
      241 aaaccggcga atatagcgac tttggcgttc cggcgacgat tctggcgcac tatctgcgtg
      301 agaacggcat tgtgccggag aagtgcgatc tcaactccat tctgttctta ttaactccgg
      361 cggaaagcca cgagaagctg gcacaactgg tggcgatgct ggcgcaattt gaacagcata
      421 ttgaggatga ctcgtcgctg gctgaggtgt tgccgagcgt ttataacaaa tatccggtgc
      481 gctatcgcga ctacaccctg cgccagttgt gtcaggagat gcacgatctg tatgtcagtt
      541 tcgacgtcaa agacctacaa aaagcgatgt tccgccagca gagtttcccg tcagtggtga
      601 tgaaccccca ggatgcgcat agcgcttata ttcgcggtga agtggagttg gtgcggattc
      661 gtgatgccga agggcgaatt gcggcagaag gggcgttgcc ttatccacct ggcgtgcttt
      721 gcgtggtacc cggggaagtc tggggtgggg cggttcaacg ttatttcctt gcgctggaag
      781 aaggcgtgaa tttgttgccg ggattttcgc cggagttgca aggtgtctat agcgaaaccg
      841 atgcggatgg catgaaacga ttgtacggtt atgtgttgaa gtaagaataa aaaaacgggt
      901 caccttctgg cgacccgttt ttctttgcgt aattagtggc taaccgtctg tgtgcctgtc
      961 gggacacgaa cgtgtttata tttgaacatc gccatgaacg cgaaggccag aaccacggaa
     1021 taaccagcga aaatcaacca tacggtctgc cagtcggtaa tgccgttttg ggtgtacatc
     1081 tcaacaactt taccgctcac gatgccgccg aggatacagc cgaagccgtt agtcatcatc
     1141 aggaacatcc cttgtgcact ggcgcgaatt gccgggctaa cttctttttc gacaaacacc
     1201 gaaccagaga tgttgaagaa gtcgaatgcg caaccgtaaa cgatcatcga cagtaccagc
     1261 agtacagtac cgaacggagt cgggtcgccg taagcaaaca gcgcaaaacg caggatccac
     1321 gccacaatac tgatcatcat tacgttctta ataccgtagc ggcttaagaa gaacgggatg
     1381 gtcagaatga acagggtttc agagatctgc gaaatcgaca tgatgattga cgcatgctgc
     1441 acgatgaagc tgctggcaaa catcggatct ttgtcgaagc tgtgcaggaa ggtattaccg
     1501 aacatgttgg taatctgcag ttccgcgccc agcagcattg agaagatgaa gaagattgcc
     1561 atacgcttgt ttttaaacag cgcgaatgca tcgaggccca gcagggttgt ccagctctga
     1621 ttcgcttgct gtttagcaac cggaatatgc ggcagagtca gggtaaacag aaccagaatg
     1681 gcggaaagtg ctgcgccaat atacagctgc atgtggctta attcgaagcc agacaggctc
     1741 accacccaca ttgccatgat aaagccgatg gtgccccaga tacggattgg cgggaagtca
     1801 gtaacgatat ccatcccggc attttgcagg cgatagtaag agatggtgtt gattaaccca
     1861 agcgttggca tataagcaaa cgagttaatc aatatcacaa ggaacatcgc ttccggcgtc
     1921 gtgacctgtg ccgccatgaa cagcgtgata gcgccaatgg tgtggcaaat ggcatatacc
     1981 catttcgcac ttaaccattt gtcggccaca atccccagca gcgcaggcat aaataccgcg
     2041 gcgataccca gtgagctata aactgcgcca atagaagcac cgtcaaactt cagggtaaca
     2101 aacatatagg agccgagggt cgtcagccaa cttccccaca gacagaactg cagaaaagag
     2161 aggattttca gctgcagctt aagattcatg ttaatttcct cacatcgtga tgcggatgag
     2221 tgtttgaaaa agcacatttg accgtgcgag atgatgggat tctatcaact tcgcggactg
     2281 gttttttgtt acctgtggca aataattgca aacattccca atttgcaaaa cgaaattgtg
     2341 atgtggataa catttttgcc cctgagcatc gtcaggggcg gttaatggaa ttatcg
//
LOCUS       DAZSNM010000350         2386 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZSNM010000350 DAZSNM010000000
VERSION     DAZSNM010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.353"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..449
                     /gene="ipaH9.8"
                     /locus_tag="ACHW42_004204"
     CDS             <1..449
                     /gene="ipaH9.8"
                     /locus_tag="ACHW42_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR8945764.1"
                     /translation="EIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRTAEAM
                     VRSREENEFTDWFSLWGPWHAVLKRTEADRWAQAEEQKYEMLENEYPQRVADRLKASG
                     LSGDADAEREAGAQVMRETEQQIYRQLTDEVLALRLSENGSQLHHS"
     gene            657..926
                     /gene="yacA"
                     /locus_tag="ACHW42_004205"
                     /pseudo
     CDS             657..926
                     /gene="yacA"
                     /locus_tag="ACHW42_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
     gene            926..1095
                     /gene="yacB"
                     /locus_tag="ACHW42_004206"
                     /pseudo
     CDS             926..1095
                     /gene="yacB"
                     /locus_tag="ACHW42_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            1178..1768
                     /gene="ospG"
                     /locus_tag="ACHW42_004207"
     CDS             1178..1768
                     /gene="ospG"
                     /locus_tag="ACHW42_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HFR8945765.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            2125..>2386
                     /locus_tag="ACHW42_004208"
     CDS             2125..>2386
                     /locus_tag="ACHW42_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR8945766.1"
                     /translation="MTEALRLHFEEHLPRVVAGCRLGVPKSTVCGMFVRFRNAGLSWP
                     LPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPRR"
BASE COUNT          665 a          528 c          597 g          596 t
ORIGIN      
        1 atgagataga agtctacctg gccttccaga ccatgctcgc agagaaactt cagctctcca
       61 ctgccgtgaa ggaaatgcgt ttctatggcg tgtcgggagt gacagcaaat gacctccgca
      121 ctgccgaagc catggtcaga agccgtgaag agaatgaatt tacggactgg ttctccctct
      181 ggggaccatg gcatgctgta ctgaagcgta cggaagctga ccgctgggcg caggcagaag
      241 agcagaaata tgagatgctg gagaatgagt accctcagag ggtggctgac cggctgaaag
      301 catcaggtct gagcggtgat gcggatgcgg agagggaagc cggtgcacag gtgatgcgtg
      361 agactgaaca gcagatttac cgtcagctga ctgacgaggt actggccctg cgattgtctg
      421 aaaacggctc acaactgcac cattcataat cacatcgcat aaaactgtca gcgccagtga
      481 taggacaggt gaaacctgtc aatacgtacc ggcttacgcc gggcttcggc ggttttactc
      541 ctctgccata tgcaacaacg gagtgccgcc ttacatgccg ctggcgcggc atatcctggt
      601 gacgatatct gaacagttat atactgtgta tatacgtggt aatgacgagg tggcaaatgg
      661 cacaggttaa tatgagtgta agaatcgacg cagaactgaa ggatgctttt atggccgctg
      721 caaaaagcat ggaccgtaat ggctctcagt taatccggga ttttatgcgc cagaccgttg
      781 aacggcagca caattcttgg ttccgtgact aggttgcggc aggccgtcag caactcgaat
      841 gcggtgatgt gcttccccat gacatggtcg aatcctctgc tgctgcatgg cgggatgaaa
      901 tgagcaggaa gattgctgat aaatgatgga gatgttctgg accatgctgg ccatccagga
      961 caggaagcgc attcgtagta cattgcggag cagaacctga tggccgccat tgaaccggac
     1021 gaacggattg gttattcagc cagcagcctg gcagggcagc cgtataaagg ccgcaacggt
     1081 cgtgtggaag gaacatctgg accacacaag gtagcgtgta acgttatttt gtgtgaaaat
     1141 ctcttgtgat ttttaaatat cgtaacaaag gtatgacatg aaaataacat ctaccattat
     1201 tcaaacacct tttccatttg agaataataa ttctcatgct ggcatagtaa cggagcccat
     1261 tctcggtaag ttaataggtc aggggtcgac agcagaaatc tttgaagatg tgaatgattc
     1321 atctgctttg tataaaaagt atgatcttat tggcaaccag tacaatgaga ttctggaaat
     1381 ggcttggcaa gaatctgagc tttttaatgc tttttatggc gatgaagcat ccgttgttat
     1441 acagtatggc ggagatgtgt acctccgaat gctgcgcgtg cctgggactc cccttagtga
     1501 cattgataca gctgatatcc ctgataatat agagagcctt tatctacagt tgatatgtaa
     1561 attgaatgag ttgagtataa tccattacga tcttaataca ggtaatatgc tgtatgataa
     1621 agaaagtgaa agtttattcc caatagattt tcgcaatatt tatgctgaat attacgctgc
     1681 aaccaaaaaa gataaagaga ttatcgaccg acgattacaa atgcgtacaa atgattttta
     1741 ttcgttatta aacaggaaat atttatagac gtatttgttg atgctataag ccagaggctc
     1801 ttctatgaga atatggggac cattccctcg cgatattaac gtaatatctt aattgtatgc
     1861 accgccagaa tgcgttcagt gcgatcacgg cagcagacag gtaaaaatag caacaaacca
     1921 cccgaaaaac cgccgcgatc gcgtatgata aattttaacc acatgcatac ctgtgcaaca
     1981 aggcgaatca cgcacggatt tccagccaga gacacctgaa acgtaaccgg ctcatttaaa
     2041 ccgtctggtc tgttacctcc ggttccacaa aaataatgtc catcattttg aagtggacac
     2101 tatcgtatgg aataccggac ctgaatcact gaagctttac gccttcattt cgaagaacat
     2161 ttaccacggg ttgtggccgg atgtcgcctg ggcgtaccaa aatcaactgt ctgtggtatg
     2221 ttcgtgcgct tccgcaacgc tggcctttca tggcctctgc ccgcaggtat gtcggagcag
     2281 gaacttgatg cccttcttta cggaagtgcc tccacagtac ctgtcgtact tactgaaagc
     2341 accgttatgc ccaaactccc ggtggtaaaa aaacgtcccc ggcggc
//
LOCUS       DAZSNM010000351         2341 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZSNM010000351 DAZSNM010000000
VERSION     DAZSNM010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.354"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..793)
                     /locus_tag="ACHW42_004209"
     CDS             complement(<1..793)
                     /locus_tag="ACHW42_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8945767.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQR"
     gene            complement(1392..1640)
                     /locus_tag="ACHW42_004210"
     CDS             complement(1392..1640)
                     /locus_tag="ACHW42_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR8945768.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1903..1998)
                     /locus_tag="ACHW42_004211"
                     /pseudo
     CDS             complement(1903..1998)
                     /locus_tag="ACHW42_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1995..>2341)
                     /locus_tag="ACHW42_004212"
     CDS             complement(1995..>2341)
                     /locus_tag="ACHW42_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR8945769.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          606 a          431 c          561 g          743 t
ORIGIN      
        1 atctttgcag ggattgcaga gcatgggatg acaatgggtt atcactaata tctattgaac
       61 attcattcct cagattaaga atgctttccg ggatatgact tatctgattt atgtcaaaat
      121 aaccgttcct taaattttcc ggcaggtatg gaatatcggt taacatattg tcgattgccc
      181 atatggagtg aatgttgtga ggtagagggg ggagttcact aagaagattc cctattgcgc
      241 taatctcttg taaagaaaat ggtaaaggtg gtaaaactgc cagcccatta catgatacat
      301 ctaatgtttc cagtagttct ggtaaaacag gaagagaaca taattgatta tttgagacat
      361 gaagctcctt caaagatatg gggagtgtgg gtagtgtgat tagttgattg tgggacgcat
      421 tcaataattt aagtccttga ggcaaagcag gcagttcaat aagtctgtta tagctgacat
      481 taagttgtgt aagggacgca ggcaatgggg agattaagct taaattattt ttacttatat
      541 taattgattt aattcccggg gggatttcag gtaatgttgt caggcctaat tcagacaagt
      601 ccaggttcgt ctcttggttt tgtagacatg atactagtcg ctgaaaagcg atgtctcgtt
      661 gttcttcttg tatgcggtta tttttccatt cagtccaatg ggttagataa ttttcatatg
      721 cgcggctagt gtcgattgaa taagtggaga acgaatttga aattaatctg tgattgttat
      781 ttgtcgggag cataaatatc agggtgtatt ctttatgttg agaggtgttg gattcttttt
      841 tatctgcttt gcaatgttat tggttccctt cagcagtaga caggaacttc tggaggaggc
      901 ggatatatag tttgtgtaag catattaact ccatatgtta tatattgaag aacttctgct
      961 ttacactatc ctaaactgga tcggtttaat gtaaaaaaac gatacttttt gggggggggc
     1021 aatttttcct taaggtatga aaaaaggagc tgaagctata taatagctat taatgccact
     1081 ggtatcagtg aatcatgtat ataaaaaact gtgttaagga ggctgtccat tagcggtttt
     1141 ttattcttct ggaaccgcgg catctttgcg tatctttttg tcttctcacc gtctggtcgg
     1201 tgttttgctg agactgctaa tttcctgtct ttgctagtgc tgtactggta ctgcccaatc
     1261 atgattggcg ggggcgggcg gagttgagac cgcagacacg tcgtatgcaa gaacgtgctg
     1321 cggctggctg gtgaacttcc gatagtgcga gtattgaatg atttccagcc gttactgatt
     1381 ttacgtgcta attagtgaac aaaccactcg tcagcagact cccaggtatc tttcagagtt
     1441 tcctgaacaa aagttttagc tgaatcttta tcggcggtgc gcgtaacaga aaggccatcg
     1501 ttgctggtgg cttttacgat cacctctaca tcgtcataac gtttactgat gcgtcgggtt
     1561 aattcttcct ttaacgcatc cacagcaccg gttggcattt tagtcatttt ctctttggct
     1621 atgcagattt caaaacgcat aaaaatccct ctgcactgtg tttttataca gtattatttt
     1681 taactgtatg gataaacagt gtcaagagtg agggaaagtg attttttggg gcatgcatgg
     1741 ggcaaaaata tttgttgtgg ggcattttgg ggcatgattg ggacactttg ttacatatga
     1801 actttgccga ttttcatatg acatggcaat ccaacgcttt gattattcta aagaaaacac
     1861 atgctcttgg gcgttcttta gtgattttta aaaatcccgt atcacgcagt taaagtggcg
     1921 ggcatactct tcaaggctgg tgatccccaa ccgcacccat ttaggatgtg accattgcgg
     1981 tagaccgatg taaatcataa cgcgtgaagg atctcttcca cgctacgcac acgggcgatg
     2041 cgcgggtaga tatgattaat gctgttattg tgttgctcgg cgctggcggc actacaggcg
     2101 tcttcggcaa tcaccagatt aaagccaagt tcccaggcat tgcgggcggt ggattcaaca
     2161 ccgatattgg tcgagatccc acataacact attgtatcga tacctcggcg gcgcagttgc
     2221 aactccagat ccgtaccgta aaacgcaccc cactgacgtt tgatgatttc gatatcactg
     2281 tcagttgcac ctaatgcagc tggatgttgc caccagtttt cgggcaacac tttagcgggg
     2341 g
//
LOCUS       DAZSNM010000352         2340 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZSNM010000352 DAZSNM010000000
VERSION     DAZSNM010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.355"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..766
                     /gene="psuG"
                     /locus_tag="ACHW42_004213"
     CDS             <1..766
                     /gene="psuG"
                     /locus_tag="ACHW42_004213"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR8945770.1"
                     /translation="GAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            860..2110
                     /locus_tag="ACHW42_004214"
     CDS             860..2110
                     /locus_tag="ACHW42_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR8945771.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2181..2340)
                     /locus_tag="ACHW42_004215"
                     /pseudo
     CDS             complement(2181..2340)
                     /locus_tag="ACHW42_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="activator of nucleoside metabolism; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
BASE COUNT          529 a          535 c          638 g          638 t
ORIGIN      
        1 gggcgcagta cctgccacta tcgccattat tggcggcgtg atgaaagtgg gtttaagcaa
       61 agaaaaaatt gaattactgg gtcgtgaagg gcataacgtg accaaagtta gtcgtcgcga
      121 tttacctttt gttgttgccg ccggaaaaaa tggcgcaacc actgtggctt caacgatgat
      181 tattgcggcg cttgccggaa ttaaagtatt tgccaccggg ggaattggtg gtgtgcatcg
      241 cggggcggaa cataccttcg atatttctgc cgatttgcaa gaactggcaa atactaatgt
      301 caccgttgtt tgtgccgggg cgaaatctat tctcgattta ggattaacca ctgagtattt
      361 agaaaccttc ggtgtgccgt taattggcta tcagactaaa gcgctgcctg cgtttttctg
      421 ccgtaccagc tcgtttgacg tcagcattcg tctcgacagc gccagtgaaa ttgcccgtgc
      481 aatggcggtg aaatggcaaa gcgggctgaa cggtggcctc gtggtagcga acccgatccc
      541 ggaacagttt gcgatgccgg aggaatctat caatgcagcc atagatcaag ccgtcgccga
      601 agccgaagag cagggcgtta ttggtaaaga aagtacaccg ttcctgctgg cgcgcgttgc
      661 tgagctgacc agcggtgaca gcctgaaatc caatatccag ctggtgttca acaacgccat
      721 tctggcgagt gaaattgcca aagaatacca gcgtctcgcg ggttaatttc tgacgggcag
      781 aaagccgtcg cccgtactca tcaagcatac caaaacctgg cgctgacgcc gggtttactg
      841 gcatgaaggg aaaatcatta tggatataat gagaagtgtt gtggggatgg tggtgttact
      901 ggcaatagca tttctgttgt cagtgaataa aaagagcatc agtttgcgca cggttggagc
      961 cgcactgctg ctgcaaatcg ctattggtgg catcatgctc tacttcccac cgggaaaatg
     1021 ggcagtagaa caggcggcat taggcgttca taaagtgatg tcttacagtg atgccggtag
     1081 cgccttcatt tttggttcgc tggttgggcc gaaaatggat gtcctgtttg acggtgcggg
     1141 ttttatcttc gcctttcgcg tacttccggc gattattttc gttactgcgc tcatcagtct
     1201 gctgtactac attggcgtga tggggctgct gattcgcatc cttggcagca ttttccagaa
     1261 agccctcaac atcagcaaaa tcgaatcttt tgttgcggtt accactattt tcctcgggca
     1321 aaatgagatc ccggcgatcg ttaaaccgtt tatcgatcgc gtgaatcgca acgagttgtt
     1381 taccgcaatt tgtagcggga tggcgtccat tgctggttcg gtgatgattg gttatgccgg
     1441 aatgggcgta ccaattgact acctgttagc ggcatcgctg atggcgatcc ccggcggtat
     1501 tttgtttgca cgtattctta gcccggcaac cgagccttcg caggtcacat ttgaaaatct
     1561 gtcgttcagc gaaacgccgc caaaaagctt tatcgaagcg gcggcgagcg gtgcgatgac
     1621 cgggctgaaa atcgccgctg gtgtagcgac ggtggtaatg gcgtttgtcg caattattgc
     1681 gctgatcaac ggcattatcg gcggaattgg cggctggttt ggtttcgcca atgcctctct
     1741 ggaaagtatt tttggctatg tgctggcacc gctggcgtgg atcatgggtg tggactggag
     1801 tgatgccaac cttgctggta gcctgattgg gcagaaactg gcgattaacg aattcgtcgc
     1861 ttacctgagt ttctccccat acctgcaaac gggcggcacg ctggaagtga aaaccgtggc
     1921 gattatatcc tttgcgcttt gtggttttgc taactttggt tctatcggtg ttgtcgttgg
     1981 cgcattttcg gctatttcgc caaaacgtgc tccagaaatc gcccagcttg gtttacgggc
     2041 gctggcagca gcaacgcttt ccaacctgat gagtgcgact attgccgggt tctttattgg
     2101 tctggcgtaa tgctcgattt gtagaccgga taaaacgtat taagcgttgc atccggcaat
     2161 cgcgctattg caaataattt ttactgcatc atcccggaga atttattctc cgggtccatc
     2221 tccagcgcca gtcctgacaa ctgctttctg tttttaatta gatatccttt cttttaatta
     2281 ataaaccgtc gtgaatgaat tgcgcgagaa catataaaag atgtcgataa gaaacgccta
//
LOCUS       DAZSNM010000353         2325 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZSNM010000353 DAZSNM010000000
VERSION     DAZSNM010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.356"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            61..282
                     /gene="kil"
                     /locus_tag="ACHW42_004216"
     CDS             61..282
                     /gene="kil"
                     /locus_tag="ACHW42_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR8945772.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            282..452
                     /locus_tag="ACHW42_004217"
     CDS             282..452
                     /locus_tag="ACHW42_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFR8945773.1"
                     /translation="MTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="ACHW42_004218"
     CDS             527..802
                     /locus_tag="ACHW42_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945774.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="ACHW42_004219"
     CDS             902..>2325
                     /locus_tag="ACHW42_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR8945775.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          720 a          531 c          528 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatattcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DAZSNM010000354         2310 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZSNM010000354 DAZSNM010000000
VERSION     DAZSNM010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.357"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..565
                     /locus_tag="ACHW42_004220"
     CDS             <1..565
                     /locus_tag="ACHW42_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8945776.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="ACHW42_004221"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="ACHW42_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR8945777.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="ACHW42_004222"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="ACHW42_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR8945778.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..>2310
                     /locus_tag="ACHW42_004223"
     CDS             2167..>2310
                     /locus_tag="ACHW42_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 family protein"
                     /protein_id="HFR8945779.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLY"
BASE COUNT          582 a          512 c          596 g          620 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgt ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gaagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctagcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat
//
LOCUS       DAZSNM010000355         2298 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZSNM010000355 DAZSNM010000000
VERSION     DAZSNM010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.358"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(581..2185)
                     /locus_tag="ACHW42_004224"
     CDS             complement(581..2185)
                     /locus_tag="ACHW42_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8945780.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          594 a          555 c          507 g          642 t
ORIGIN      
        1 ctttaattgt gtctttaagt taactttaag ttaagcccct gtagggacta gagaccataa
       61 ttaagatgat tgtgtcttat tgctattcac ttatcgactg acccagagca cacaggaagc
      121 attcacttta agtagaacgc ttggtgtctg taattggtca tcccgcccct gaaaggatgc
      181 gataagttac ggttagttgt cctagaagag aaaacttttt gtgtcccttc tccctatagt
      241 gagtcgtatt aattatcctt gacaagtaga gaaaaccatg agtcaaaaac atgaccacac
      301 aggtctgtag taagtgtcat cctagaagag aaaacttttt gtgtcccttc tccctatagt
      361 gagtcgtatt aattacccct tgacaagcag agaaaactat gagtcaaaaa catgaccgca
      421 caggtctgta gtaagtgtca tcctagaaga gaaaactttt tgtgtccctt ctccctatag
      481 tgagtcgtat taattatccc ttgacaagta gagaaaacta tgagtcaaaa acatgaccac
      541 acaggtctgt agtaagtgtc atcctagact atggtctcct ctaggtctcc ttataaacat
      601 cagccatcac attgaccagc ctatcaacct cgacctcttt agcataacgg tcgtacgtaa
      661 tagaccctga actatgcccc atgatcgcct gtgcatacgc caactcgacc ccatgttgtt
      721 ttaactgggc cgcaactgta tgtctaaacg agtgaaacgc taagccgcta cggtctacga
      781 ggactttagg caacaacgtc cgattaaacc atttactcac ctgctcaccg taaccgttct
      841 tcatgagacg cagaccatcg aacaccatag cgtcatcacc gtcagcgcct ctacgggtct
      901 ctacaagcgc cataaagtca gccaatataa aaccataggc cccatcgacc agaggcacac
      961 agcggtcact atgagcgttc ttgatgctct taccgggtag actactatcg tcttcattga
     1021 tatgaatata aacggttccc gcctcagtgg tcctgacgtc tttgacctgt agttgggcta
     1081 tttcattgag tctcgcccca gtgatagcag ccaaggcggt aacataataa tgatacggct
     1141 taccagaggt cttctgagag tatgccttag ctgcgaccag tagttgcccc acctgctccg
     1201 tagagaaggc gttacgggcc cctgaggctt tacgctgagg gactttaagc tccagaccct
     1261 cagtcatgtt ctttttaatc aagtcattgc gcacagccca cctaaagaca gcagccatct
     1321 taataagata cttgttgttg atggtaacga catccagaca atctgtctta tcttcacggc
     1381 tcaacaggtc agcaagtggg acatccttga agcgctgctt acggttccta ggtaactgct
     1441 ggagcacatc acggacccta agcatgtcag ccctgttagc gtctgcattg agacccagat
     1501 ggtcgaatat ttcgattaac gcagcgtgtg acgccttgtt ttcccttaag gttgcaggct
     1561 tccagttctg atagttctcc gcttcgtatt gttcaaagag gctcgctatg gtaatgctag
     1621 gcgtaacttc tggttttact tctggagcat taacagatag ggacacagag tctgcttggt
     1681 tatcttgcct atcaatatca ttaaaaagat cgacgtaatc tatcagaggc tgactgtctc
     1741 ccgcttcgat acgcctcata caggctttta gcaccttcag ggcctcattg atgtaacgat
     1801 gctggtcaat gcttagcggc tcactagcca gagcatcggt aaggctctca ccaagttctc
     1861 cgtattggtc ccggtagata tccctcatgt caggctcaaa gagatctgaa cggcccatag
     1921 acagttcaca ctcagcgatg tccttaaggt gttccctcat ttcctcatag gtagcgttgg
     1981 gtctgtctgc gtgtattgcc ttgagtgctg cctttatgtg ccttgagtaa gccatagcgg
     2041 tgcgtctatc cttggtccgt aatgatattg aggtcattct atcattgttt gattgtctga
     2101 ccctaagacg gaagtagtag acgccgttac gctggtagag atagtagact gctggaagtt
     2161 tgtgactact ggatagctga atcatatgtc tgcatgccct gagagggtta cacagtgggt
     2221 tacagtaagt aactctgcaa ggaggcttaa agccgtatcc cttaggattc agtaggttgg
     2281 agtactttaa gaaaggtc
//
LOCUS       DAZSNM010000356         2290 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZSNM010000356 DAZSNM010000000
VERSION     DAZSNM010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.359"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1224
                     /locus_tag="ACHW42_004225"
     CDS             <1..1224
                     /locus_tag="ACHW42_004225"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR8945781.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1958
                     /locus_tag="ACHW42_004226"
     CDS             1224..1958
                     /locus_tag="ACHW42_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8945782.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
BASE COUNT          587 a          462 c          576 g          665 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgcttg caaatatagg tgatttttta
     2221 ttgtaggctt ttgaaggcta ttataactgt gcgctaaatt aatgcgttga ataaaagaaa
     2281 taacaaaatt
//
LOCUS       DAZSNM010000357         2244 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZSNM010000357 DAZSNM010000000
VERSION     DAZSNM010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.360"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(158..670)
                     /locus_tag="ACHW42_004227"
     CDS             complement(158..670)
                     /locus_tag="ACHW42_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR8945783.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            865..1629
                     /locus_tag="ACHW42_004228"
     CDS             865..1629
                     /locus_tag="ACHW42_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945784.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1580..>2244
                     /locus_tag="ACHW42_004229"
     CDS             1580..>2244
                     /locus_tag="ACHW42_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR8945785.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          642 a          490 c          565 g          547 t
ORIGIN      
        1 atcatcagcg accagacagc attgagaatg cttcaggagt acatcaggat ccaatgcctt
       61 ggggggtagc ggtaatttta ctcattatcc ttcaaatcag attctgttgt cagaggaatg
      121 ggggacgctg gattcgcaag agctttttat gggcagttta gctaaaaaaa ggcaacacga
      181 agctaaaact accaacataa agtgctgacc agctagcagc tgaaatcaga ctcactgtat
      241 acactttcac tggatgaagt tgcaacattc ctgccaccaa aggagcaatg taacgcagca
      301 cggcgataaa gcgcgaagtg aaaagtattg acacagagtt attttgcaac tgaaggcgaa
      361 cccgttctat tgtattggaa cggctcgata ttatacatgc aagttgaggg atatgcccaa
      421 ttattatacc aagatggtaa ttaacgatag taccgcacca tgcgccactc atgaccgtga
      481 ttccagcttc ccatggtgaa agagtcgtct tgctgactgt aattacagtg gccatcataa
      541 ttgatgctgg tggcaatatc gccgaaatga gtaaagtgga ttttgtgaac gctattataa
      601 aaagaagccc ccataggcga tatggatgaa gtacaaaata gttcatcaaa gcatttatcc
      661 actccatcaa tgtctttcct tctgtgaatt tttctttgct atttaaccgt gatacgactt
      721 aatttaggct taaacacctt acatttacaa ggtactcctg tggggggccc tgccacggag
      781 cgtcgcagga gtgagtgatg aggaagaatt aaagcaacct attgtgacag gcagcgataa
      841 cagtaaatca caggaagcat aatcatggca aaaccggact gggaggccat cgaatcggca
      901 taccgggccg gagtccttag cctccgtgat ataggcgaga aatacggcgt tacagaaggg
      961 gctatcagga agagggccaa aaagcttgat tgggcacgca gtggcggtac gcaggtttgc
     1021 aaaaatggta cgcaaaaaag gaaagtgcgt accagcagaa agcctgccat tactggcctt
     1081 acacaaaaaa gtacgcaact aaaaacagaa tctacaccgg atacgaaacc gatacgcgga
     1141 atgcgtaccg atcccccgac taacccattc caacccggta accagcaggc attaaaacac
     1201 ggtggttatg cccgtcgcct tctgctcaaa gatgaggtga tagaggacgc taaagcgttg
     1261 acgctcgagg acgaattatt tcgccttcgt gctaacaacc ttgtcgctgc agagaatatt
     1321 ggtcggtggt tggtgtcgct ggaagatgct aatggggacc aggaaaggaa gatgctgatt
     1381 gaaaatatca gcgccgccga gaaagcaatg atgcgcaata cagttcgtat tgagtccatc
     1441 gttggcacgc ttgcgacggt aggaaaaata tttgctgata cagcctaccg caaggccgcc
     1501 actgataagg tgtctctgga ggctgatcgt cttcgccgtg atgcaggtat tgatgatggc
     1561 aatggagagc gtgacctcaa tgacttctac tctgacatcc aaaccgacgc tgaatccggc
     1621 tttacgtagt ttctggacta tgcgggcacg taacaaagtg ctttatggtg gtcggtcatc
     1681 gtcaaaatca tgggatgccg ctggcattgc catatttctg tcgaataaat acaccctgcg
     1741 tttttgttgt gcccgtcaga tccagaataa aatcgaagag tcggtgtata ccctgctcaa
     1801 aattcagata gacaggtttg gtctgcggca ccgtttccgt attctgaaca acaaaatcat
     1861 taaccgggtt actggctcgg aatttgtttt ttatggatta tggcgcaaca tcgaagaaat
     1921 taagtcactg gaggggatcg atatgttgtg gttggaagaa gcccacgcac tgacggaata
     1981 ccagtggaaa attctggagc caacgatccg taaagagggt tcggaatgct ggttcatatt
     2041 caaccccgga cttgttactg atttcgtctg gcgcaacttc gttgttgatc cgcccgaagg
     2101 cactctcatc cgcaaaatta actatgacga aaatccgttt ctgtctgaca ccatgcttaa
     2161 ggttatcgac gcggcgcgac gccgtgatcc ggatggtttt aaacatgtgt atgagggcgt
     2221 tccggagtct gatgatgatg cggc
//
LOCUS       DAZSNM010000358         2230 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZSNM010000358 DAZSNM010000000
VERSION     DAZSNM010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.361"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..222)
                     /locus_tag="ACHW42_004230"
     CDS             complement(<1..222)
                     /locus_tag="ACHW42_004230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945786.1"
                     /translation="MFFSLSVLNLEDHLGLVISPQSPLSAGYITLRACRRSLPLRFST
                     VTPGGIVGFLIVSYRFNIPDRLAVHLSIHA"
     gene            651..2159
                     /gene="ltrA"
                     /locus_tag="ACHW42_004231"
     CDS             651..2159
                     /gene="ltrA"
                     /locus_tag="ACHW42_004231"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR8945787.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          572 a          548 c          657 g          453 t
ORIGIN      
        1 ggcatggatg cttaaatgaa ccgcgagtct gtctggaata ttgaaccggt aactcacgat
       61 gagaaaccca acaatcccac cgggtgtgac ggtggagaac ctgagcggca gtgacctgcg
      121 gcatgcccgc agggtgatgt aacccgctga caacggggat tgaggcgaga tcactaagcc
      181 gagatgatcc tcaaggttaa gtactgaaag gctgaagaac atgaacccgt taatccgcct
      241 ctgtgggttg aaaacgtcac cacggcctac gtgatctgac aggccgtgca ggaggaactg
      301 gcagtgatac gtaagcactg ccggtcgaag gtgttttgac atgtatgcga aacaccgggg
      361 cagcagcgtc tatcacactt gcgttgctga cttctgccaa cttgcggcaa gcaaggataa
      421 agagtgcgac gggcagcctc ctcagtatgc ctgagtccag gcaggtaaac cggggaaggt
      481 cagcgacgga tgttaagggg gcatggctcc gatgacgcgc tggctggcgg agcttccgta
      541 gtagtccgcg atggggaaag cccattacat ggcgaaggga agcagtttga atgtgtttgc
      601 gacgtgaatt aactgaccta acgaggtgaa gacctttgat aatcagcgaa atgcaacgca
      661 agcttgccac atgggcagcc accgatccgt ccctacggat tcaacggctg ctgcgtctga
      721 taacacaacc agaatggctg gctgaagcgg cgcggatcac gctttcatca aagggggccc
      781 atacccccgg cgttgatggc gtgaacaaaa caatgctaca ggccagactg gctgttgagc
      841 tgcaaatcct cagggatgaa ttactctcag gccactacca gcccttgccc gccagacggg
      901 tttacatccc taaaagcaac ggcaaactgc gaccactggg tatccccgcg ttgcgggatc
      961 gtattgttca gcgggccatg ctgatggcga tggagccgat atgggagagt gattttcata
     1021 cgctctcata tggcttccgg cctgagcgca gtgtccacca cgcgatccgc acggtgaaat
     1081 tacagctcac agactgtggt gaaacccggg gacgctgggt gattgaaggc gacctgtcca
     1141 gttacttcga caccgtacat catcgactgc tgatgaaggc tgtacgccgc aggatcagtg
     1201 acgcacgttt catgactctg ctgtggaaaa ccatcaaggc gggacatatc gatgtcggtc
     1261 tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt tatatcgccg ctattatcga
     1321 acatcatgct gaatgagttc gatcaatacc tgcatgagcg ctacctgagc gggaaagcca
     1381 gaaaagatcg gtggtactgg aataacagta tccaacgggg ccgaagtacg gcggtcagag
     1441 aaaactggca gtggaaaccc gcggtggcgt actgccgcta tgccgatgat tttgtcctca
     1501 tcgtcaaagg caccaaagca caggcggaag ccatcaggga ggagtgtcgg ggtgtgctcg
     1561 aaggcagtct gaaactcagg ctgaacatgg ataagactaa aatcacccat gttaatgacg
     1621 gctttatctt tctggggcac aggatcattc gcaaacgcag tcgttatggc gagatgcgag
     1681 tggtctcaac gatcccgcag gagaaagcca gaaacttcgc cgcatcgctg acagcactgt
     1741 tatcaggcaa ctacagtgaa agcaaagtcg atatggctga acaactcaac cgaaaactga
     1801 aaggctgggc catgttctat cagttcgttg attttaaggc caaagtcttc agttatatcg
     1861 accgtgtcgt gttctggaag ctggctcact ggctggcccg caaataccgt acaggtatcg
     1921 cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg tcagagcaaa acgtgggttt
     1981 tatttggtaa aaccaatcac ggcaagctca gcggcgaaat actgtaccgg ttggtggggc
     2041 aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa tccctatctg aggacggaga
     2101 ccagaaacac gtatacatcg cgctttacag aagtggcaat ggcgttcgcc agcatttaaa
     2161 tggagagccg gatgcgctga aaggtgcacg tccggttcgg ggaggagagg cagggaaata
     2221 gtccgactac
//
LOCUS       DAZSNM010000359         2218 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZSNM010000359 DAZSNM010000000
VERSION     DAZSNM010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.362"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACHW42_004232"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACHW42_004232"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR8945788.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACHW42_004233"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACHW42_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR8945789.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACHW42_004234"
     CDS             complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACHW42_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR8945790.1"
                     /translation="MQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRH
                     LALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNC
                     SGDSKNICS"
BASE COUNT          589 a          611 c          555 g          463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgg
//
LOCUS       DAZSNM010000360         2215 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZSNM010000360 DAZSNM010000000
VERSION     DAZSNM010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.363"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(55..324)
                     /locus_tag="ACHW42_004235"
     CDS             complement(55..324)
                     /locus_tag="ACHW42_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR8945791.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            381..1049
                     /locus_tag="ACHW42_004236"
     CDS             381..1049
                     /locus_tag="ACHW42_004236"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR8945792.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1365..>2215
                     /locus_tag="ACHW42_004237"
     CDS             1365..>2215
                     /locus_tag="ACHW42_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8945793.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIW
                     HAFEHEE"
BASE COUNT          667 a          498 c          416 g          634 t
ORIGIN      
        1 cagccaaccg cagcacgtcc tgcatacgtc gtgtctgcgg tttttctttt tcgcttacat
       61 tgtgtctggt tcttccggcc actcaatatc aggtgcagtt gatgtatcaa cacggttcag
      121 caacacccga tacttcttcc aggcttccag caacgagttt tcttcctccg ttgcgatctc
      181 cagatctaca gcatcctgaa gtggcgcaat atgctcactg aatccctgga tgtagaactg
      241 tgtggtgacg gtcttccagc cattcggctc ctgctgtatc gaagcatacc aggctatttc
      301 aatatcgcta tgctgcggca gcatttaacc ccttgtaatt catcgccata attgatttaa
      361 ttcacaaata aaactataat atggtgaaat taatgaaaaa aaacacagat gatggggcta
      421 aaatttacac accacttacc ctaaagcttt atgactggtg ggttttggga gtatcaaatc
      481 ggcttgcatg gggatgtcct acaaaggaac accttcttcc acactttctg gaacatttag
      541 gtaacaacca tctggatatt ggtgttggaa ctgggtttta ccttactcac gtacctgaga
      601 gtagtctgat atctttaatg gatttgaacg aagctagcct gaacgcggca tctacaaggg
      661 ctggggaatc aaaaattaaa cataaaatta gccatgatgt ttttgaacct tatcctgcgg
      721 cgttacatgg tcaatttgat tccatttcca tgttttacct tcttcactgc ctgcctggaa
      781 atatatctac aaaaagctgt gtaatacgca atgcggcgca ggccttaact gatgatggaa
      841 ctctatacgg agccacaatt cttggcgatg gagttgtgca caatagcttc ggtcaaaaac
      901 tgatgcgcat ttacaatcag aaaggcatct tttcaaacac aaaagattcc gaagaaggct
      961 taacacatat actctcagag catttcgaga atgttaaaac caaggttcaa ggtactgtag
     1021 taatgttttc cgcttcaggg aaaaaatagc atccaaccgc agcacgttct tgcttaagac
     1081 gtgctacggt ttcctttacg atttggggtt gcgactttac ctctattgat aatgcattcc
     1141 ggcagaacgt tcaaatatga acgtacgata tttaactaac cgaaaaacaa aatatagcac
     1201 aggcgagaca tttaccatct aagaaaatag tatcgttttt ttacagttaa atcaacatca
     1261 cttccttaaa atgaaaacaa taaatttcaa tcctgaaaca ttttatttca gcttattatt
     1321 ccccagtatc acattttatc aggatatcct tctgcgaggt tattatgctt cctgtaaata
     1381 atccccccct atccactgga aacgtctctt tttacagaac tacatcaatc gacaatgttc
     1441 acaataatta tctctccgaa tgggttgaat ggactaaaaa cagcatttcc ggagaaaaca
     1501 gggaaactgc ttttacccgg ctccaattat gtctggagaa cagtgaaaca tcgttggact
     1561 tatcttgttt aggtctcaga tctctaccac gattgcctga caatcttgat gaaattaatg
     1621 taagcaataa ccaactatca atgctccccg agctaccaag ggcattgaaa gagctgaatg
     1681 caagcagtaa tcaattatct gcacttcctg aattaccagt gtcgctggaa tatataaatg
     1741 tgagtgataa ccatttgttc gcacttcctg aattacctgc gtcactagaa tatattaatg
     1801 taagtgacaa tcacctgtct gtacttccga ggttaccaat gtcattggaa ttacttgatg
     1861 cagccagaaa tgctttggaa gtaataccag attttccaga aagagatgat catattataa
     1921 gaatattctg gcttaatcag aaccggatca cggcaattcc ggaaagcata cttggcctca
     1981 gttctgatag cgttgtcaat cttagagaaa atcaactatc tcccagaata atgcaaactt
     2041 tgttacaaca aaccgcccaa ccggactacc acggcccacg gatttacttc tccatgagtg
     2101 acggacaaca gaatacactc catcgccccc tggctgatgc cgtgacagca tggttcccgg
     2161 aaaacaaaca atctgatgta tcacagatat ggcatgcttt tgaacatgaa gagca
//
LOCUS       DAZSNM010000361         2204 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZSNM010000361 DAZSNM010000000
VERSION     DAZSNM010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.364"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="ACHW42_004238"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="ACHW42_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR8945794.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1861)
                     /gene="phoA"
                     /locus_tag="ACHW42_004239"
     CDS             complement(446..1861)
                     /gene="phoA"
                     /locus_tag="ACHW42_004239"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR8945795.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1962..>2204)
                     /gene="iraP"
                     /locus_tag="ACHW42_004240"
     CDS             complement(1962..>2204)
                     /gene="iraP"
                     /locus_tag="ACHW42_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR8945796.1"
                     /translation="ELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQNDQQRLID
                     QVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
BASE COUNT          485 a          586 c          527 g          606 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagcttgt tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca tcggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
     1561 gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag
     1621 taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat
     1681 cgctaagaga atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg
     1741 gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg
     1801 cttttgtcac aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca
     1861 ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg
     1921 gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgaca aggatgcttc
     1981 aataacttct ttacgtaatc gcgcagcagc tctgtatcgt cgtcaggaat gctggcatcg
     2041 ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc
     2101 gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt
     2161 tctttcgact cttcttcttt ttgggcaagc ttaaataaca actc
//
LOCUS       DAZSNM010000362         2187 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZSNM010000362 DAZSNM010000000
VERSION     DAZSNM010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.365"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(220..384)
                     /locus_tag="ACHW42_004241"
     CDS             complement(220..384)
                     /locus_tag="ACHW42_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945797.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="ACHW42_004242"
     CDS             1286..1570
                     /locus_tag="ACHW42_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR8945798.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="ACHW42_004243"
     CDS             1558..2043
                     /locus_tag="ACHW42_004243"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8945799.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          462 c          620 g          540 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataa
//
LOCUS       DAZSNM010000363         2183 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZSNM010000363 DAZSNM010000000
VERSION     DAZSNM010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.366"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..203
                     /locus_tag="ACHW42_004244"
     CDS             <1..203
                     /locus_tag="ACHW42_004244"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945800.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSIH"
     gene            293..694
                     /locus_tag="ACHW42_004245"
     CDS             293..694
                     /locus_tag="ACHW42_004245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945801.1"
                     /translation="MNGNTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDY
                     LPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSR
                     DGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            696..1301
                     /locus_tag="ACHW42_004246"
     CDS             696..1301
                     /locus_tag="ACHW42_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFR8945802.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2183
                     /locus_tag="ACHW42_004247"
     CDS             1288..>2183
                     /locus_tag="ACHW42_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8945803.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          609 g          536 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga acgccctggg ggggaattca
     2101 attgttcttg gtgataatga caccggattt aaacagaatg gagacggtat tctggatgtt
     2161 tatgctaata gtcagcgtgt att
//
LOCUS       DAZSNM010000364         2151 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZSNM010000364 DAZSNM010000000
VERSION     DAZSNM010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.367"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="ACHW42_004248"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="ACHW42_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HFR8945804.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="ACHW42_004249"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="ACHW42_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="ACHW42_004250"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="ACHW42_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2061..2151
                     /locus_tag="ACHW42_004251"
                     /pseudo
     CDS             2061..2151
                     /locus_tag="ACHW42_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          630 a          398 c          493 g          630 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttag ttgtaattta
      901 tttatggagg ttttctatgg aattcttttt ttattgtggc tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aagccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgaca aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt caggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gtcctggcgg tatcacggcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgagtt ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct
     2041 tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa
     2101 aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata a
//
LOCUS       DAZSNM010000365         2137 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZSNM010000365 DAZSNM010000000
VERSION     DAZSNM010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.368"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            126..995
                     /locus_tag="ACHW42_004252"
     CDS             126..995
                     /locus_tag="ACHW42_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR8945805.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="ACHW42_004253"
     CDS             complement(1198..1551)
                     /locus_tag="ACHW42_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR8945806.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..>2137)
                     /locus_tag="ACHW42_004254"
     CDS             complement(1689..>2137)
                     /locus_tag="ACHW42_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HFR8945807.1"
                     /translation="HATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVAL
                     RAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRS
                     ALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
BASE COUNT          561 a          555 c          494 g          527 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgca
//
LOCUS       DAZSNM010000366         2097 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZSNM010000366 DAZSNM010000000
VERSION     DAZSNM010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.369"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..602)
                     /gene="murI"
                     /locus_tag="ACHW42_004255"
     CDS             complement(<1..602)
                     /gene="murI"
                     /locus_tag="ACHW42_004255"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR8945808.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFF"
     gene            complement(636..2096)
                     /gene="gadC"
                     /locus_tag="ACHW42_004256"
     CDS             complement(636..2096)
                     /gene="gadC"
                     /locus_tag="ACHW42_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glutamine/gamma-aminobutyrate
                     antiporter GadC"
                     /protein_id="HFR8945809.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
BASE COUNT          713 a          420 c          384 g          580 t
ORIGIN      
        1 tcaaaaaaat tctgaacaat tggatagtga gtacagccga gaataatttc atttactgga
       61 tggagggaaa ttaaatggtt aacatgtttt ttgatgtcat tcaagattgc aggagcatta
      121 agattacctt cctcaattaa cattgcaaga tttttgcttg gttctccaaa tacatttatt
      181 gttgaattca atttttttat tagctctttg tgacaccccg ttttaatagt aaactcggta
      241 gcgataatac caacattttt atattgctgg cgaatcacat cttctactgc aggttcaatg
      301 atggataaga taggaaacgg aaagctctta gcatatccgc tgtattcttc ggattccaaa
      361 gttgaggata tagtattaca tgcgacagca actagtttga cattccttga ctgtaaaaaa
      421 gtcagcattt ttttagtcaa actgatgata tcatcggcag aacggttgcc ataaggtgca
      481 ttcatattat ctccacaata gataatatct tcaccaggaa gcaaattaat tatttccttt
      541 accgatgtca atccaccgac acctgagtct attacaccaa ttggagattc agcactaaac
      601 atatttaacc tcatcttttg tagcttatgg aaaatttaat tactagttct tttttcccaa
      661 gaaaatggtt cgaaatcaga gtttggatct ttccagtcag attttcggaa ggcgtaaatt
      721 aatattggaa ttaataaaaa tagggcaact aatactaaca aataaagaac atagctggtt
      781 ggactaccag ttgggatttg ctcgggcgga ataaaactta atacaaaagc cagaaaagac
      841 cctaagaatc caagtgttgc ccagaaaaat aaagcagggg ctcgatatgg gcgttttgta
      901 tcaggttgag aatgtcgtag atagatagca ctagcgaaca tgagcatata cataactaaa
      961 tagagcataa ctgttaactg tgataaaatc tgaaaagctg cttgaactga aggcattatc
     1021 acaaaagtaa cagaaagaac tgagacaatt ataccttgta caaacattag atggtgcccc
     1081 attccatgac gattggtttt ttggaaaaac ttaggtaaat aaccagcttt cgcgatcgct
     1141 aatacacctg tattaggccc tgcaatccaa gtaaccaccc cacccagtac gccaattgct
     1201 agcataaatg ccatcacaga gccaagccat ggcactccag cccattcgaa cagcatatcg
     1261 taggcaacca acaaactttg agttagacta attttatctg taggaataat aaaggcgacc
     1321 cctaaagtac tgagcaggaa tattgcaata gtacctatag aagcgatggt aattgcaatt
     1381 gggtattttt tagttggatt atctaaatcg ttaacatgga ccgcattcat ttccatccct
     1441 gcataagcaa gaaatatgct ggcggcaaga acaatattct ggaagttagc tatattaggg
     1501 aaaagatcac cccaacctaa tgggatcacc gctggtttac cgctgaacca cagataggag
     1561 aaaccaagaa taacaaggat aatagctgga ataattgtcc ctataatacc gcaccactta
     1621 gatacctgcg agaaaatacc aacgccacgt aatgcaacac aaatagctcc ccagtaaaca
     1681 aatagtacga aaaataaaat ataccattta ttactggcaa ttgattctgc cacccccata
     1741 tcaacatcaa taaatgcaac cgagactgca ccaaatgtca gtactgttgg aaagtagggg
     1801 atgacttgta gccaggtcat aagcattcct acaaaacccc agcgagcccc aaatgcttct
     1861 cctatccatc gaaatgcccc ccctttttgt ggatatgttg tagctagctc agccgcaaca
     1921 aaggcaacag gtactaaaaa cataattgca gcaaaggtat agtaaaaaat ggcacctaag
     1981 ccgtattctg cttcagaagg tataaatcgt agacttacta cagcagttat attcatgata
     2041 gcgagtgtcc ccactgtcat tttcttactt ttgggtgcga tattagcatt agacatt
//
LOCUS       DAZSNM010000367         2085 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZSNM010000367 DAZSNM010000000
VERSION     DAZSNM010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.371"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..797
                     /locus_tag="ACHW42_004257"
                     /pseudo
     CDS             1..797
                     /locus_tag="ACHW42_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            912..1253
                     /locus_tag="ACHW42_004258"
                     /pseudo
     CDS             912..1253
                     /locus_tag="ACHW42_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1236..2073
                     /locus_tag="ACHW42_004259"
                     /pseudo
     CDS             1236..2073
                     /locus_tag="ACHW42_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          515 a          504 c          631 g          435 t
ORIGIN      
        1 tgggccttgc ccgtagcagt tatttttatc accgggctgc actgaaagcc ggtgataaat
       61 acgccacgat acgtaagatg ctgacagata tctttaacag taattaccag tgttatggct
      121 atcgtcgcct gcatgcgatg ctcaggcatg agggggggcg gctatcagaa aaggttgtac
      181 gcagacttat ggtggaggaa cagcttgtcg tcagtcgtaa ccgtcgtcgt cgctacagct
      241 catattgcgg agaaatcgga ccggctccgg ataaccttat cgccagagat tttaaggcgg
      301 agcaacctaa tcagaaatag ctgacagata tcacggagtt ccagctccct gcaggtaaag
      361 tctggctgtc accagtggtg gactgcttcg atggaaaagt tgtgagctgg tctctcagta
      421 cacgccctga tgctgaactg gtcaacacta tgctggatag cgctgtcgaa acgttaaatg
      481 ctggcgaacg accggtgata cacagtgaca gaggtgggca ttatcgctgg ccaggctggc
      541 tggaaagagt gaatgcagca ggtcttattc gctcaatgtc ccgtaaagga tgttcacctg
      601 ataatgctgc atgcgaaggc tttttcggca gactgaaaac ggaaatgtat tatgggcgta
      661 aatggtcggg catcacgcca gaaaagttca tgcagcaagt ggatgcttac atcagatggt
      721 ataacgagcg gcgtataaaa ttatcgctgg gtgccgtcag ccccaaaatg taccgccaac
      781 aatgcgggct ggaatgataa agcagtccag gaaatcgtcc gcatcccccc ttcaccattt
      841 ggattgtccg ctccacccaa catgttgttt ccttaaggtt cctacaccac aaaggaaact
      901 ctcatgctgc gcagtggatg gctgacgttg ccgacaggcg ggaacttcgc cagttcaggc
      961 agacaccgga acagcgcttc acgcaggaac aggagcatct gcagccgctg ctgggtacgg
     1021 acttcgatat ccgtcatgtc tcctgggatg gctatatcga ggttggcggg aaccgttaca
     1081 gtgtgccgga aagcctgtgt ggtcagctgg tatcaatacg gatctcgctg gatgatgaac
     1141 tgcggatcta cagtaatgag caacaggtag catcgcaccg tctctgttcg gctgcatacg
     1201 gctggcagac cgtgccggag catcacgctc cactctggca gcaggcttcg gagcgcatgg
     1261 ccgaatggct gggggagata cagaaacggg taatcactgt ctgtgaccgg gaagcggata
     1321 tctggcatta cctgtattat aaagtcagcc acggacagcg tggtgcgtgc tgcacagaat
     1381 cgccggctgg aagaggcacc cggcaagctc ttcgaactgc cggaagtcct ggcaaccgca
     1441 ggaagccaca cgctgaatgt gatgcaaaaa ggcgggcggg cagcccgtca ggcccggatg
     1501 ttcatcagtt acagcgaagt cagcataaaa aatcccgaca acagcggcca ggcgctcccg
     1561 ctcacgtatg tctgttgccg ggagcaggca gaggacggtg cctgctggca tctgctgacg
     1621 tcaggaaaag cggcgagtgc cgccgatgca cgacgtattg tcagccatta cgagcgacgc
     1681 tggctgacag aggaatacca caaggcgtgg aaaagtggtg gtacatggaa tcgctgcgaa
     1741 tgcagacccg ggataacctt gagcgcatgg tggttatcct ggcgtttata gcggtacggg
     1801 tgctgggcct gcggcaggga ggtgtcagtg aagaaacaca aaatgacagc tgtgagaaaa
     1861 tactgacgcc gacagagtgg aaactactgt gggttaagct ggaaggaaag ccgcttccgg
     1921 tgcaggcacc gaccctgaaa tgggcctggg gcgatggcat gacagcaaac gcacaggtcg
     1981 tcccggttgg ggcgtcatgt gggatggctg gtcagacttc aggatatggt tgagggttac
     2041 ctggtgatga agtctcttga tcaggagctc tgatcaagag acagc
//
LOCUS       DAZSNM010000368         2071 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZSNM010000368 DAZSNM010000000
VERSION     DAZSNM010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.372"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..337)
                     /locus_tag="ACHW42_004260"
     CDS             complement(<1..337)
                     /locus_tag="ACHW42_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR8945810.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGM"
     gene            complement(334..867)
                     /locus_tag="ACHW42_004261"
     CDS             complement(334..867)
                     /locus_tag="ACHW42_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR8945811.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(933..1562)
                     /locus_tag="ACHW42_004262"
     CDS             complement(933..1562)
                     /locus_tag="ACHW42_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945812.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1566..1781)
                     /locus_tag="ACHW42_004263"
     CDS             complement(1566..1781)
                     /locus_tag="ACHW42_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR8945813.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          531 a          551 c          452 g          537 t
ORIGIN      
        1 ccatgccgga ggcgctggag gtttcggctg aggatggcac agggcatttt cctttgacga
       61 gcacccgacc accattatca agcttgcgcc gaagagcatc attttcagct ttcgcatcag
      121 ctaactcctt cgtgtattta gcatcgagtg catcagcatc acgctggcgc tgctgcatgt
      181 cagtaatggt tgcgttcgcc agcttcagtt ctccggcgtt tttgtcgcgc tgctctttgt
      241 aggtaatggc gttatcacgg taatgattaa cagcccatga caggcagacg atgatgcaga
      301 taaccagagc ggagataatc gcggtgactc tgctcattga tctatccccc aacaggctaa
      361 tgcgctttcc tggtcacgac gaataacctg tccatagcag ttatttgaac gtatgcggca
      421 atcgcgccca acatctttta tccaccagcg aatcgcctcg catgcgccct tacgatcacc
      481 ggcattcagc cgcttataaa acgtcgacgg gaaacactta ccggggccaa tgttataggg
      541 gcaaaatgac gctatacccg ctttctgggg ttcggtcaga ggtactttaa tattgcgctc
      601 cacccatgcc agcgccttat cacgttcaat agcgttaacc tggtcacatt tttccttcga
      661 cagcttcatt cccggtatga caggcttacc atccaccatt gtggcaccac gacagatggt
      721 ccatatgcca gaaccatcac ggtatgccgt tgtgtgatta ccctcttttt cgttcagaaa
      781 ctggtcaagt atctggggag cagacgcgcc tgcggcaatc agcgccagaa cagcagccga
      841 caggccgtat ttgatttttg cgctcatgga tatttatcag gatttatcgg caacaaataa
      901 cgagccaact tatatacgcc ttttaagata agtcagcccc ggctggatcc agtcatcagg
      961 ctctttctta aagggggagt attgaaatca cgaagaagag cctcccgcac cgcagcatcc
     1021 atgtctgcac cactggcaag tgcttcaatc tctgccgcca cctgcaaata ccccatgcaa
     1081 cggccaatgc gtttcataac tctggtgttt tcgtctttca ggcatctgat agcgagatta
     1141 ataattttca ggcagtgatg atgcgatgcc cggacaggtt ttccgttctc ataaacgacg
     1201 aactcaccct cttcacagga aaccctgaaa ggccgatggg gttcctggtc tttatccggt
     1261 gcagacgcaa taaaatcact tttatgctct tcctctccgg cactgttaac cgtaatgacg
     1321 tactcaggcc gcaggtaagc cgcctctttt tctgacaatg cggccggtaa aaacttttcc
     1381 tgcaaaaact tcataaaagt ctcgtgagcg actttgtggc agtaatcgta aatcgccgca
     1441 taatgctcat cgcggcgttt gttttcatct tcagaactca ccaacgccga caatttttta
     1501 ttaagctcag tgatttcatt ttccaggtga ttgaatcgct gattcatttc ttcatgcttc
     1561 attatttact ctccccgtgc agccttacgc ttgtcttctc ttattttgaa atacagattc
     1621 gtcagataag tcagaagccc cagaagcaga cttcccagca caccaatcgc agcccactgt
     1681 gatggcctga cctgatccaa ccactgcaaa aaccagtagc cggcactgcc ggcggaggtg
     1741 ccgtaggcaa tgcccgttga aattttgtcc atggatttca tagcctcacc tccgcaaata
     1801 acggatggcg tagttttaca ctgagaaatg aaagggattt gaaaagaaaa aaacgcaaaa
     1861 gcgggcgaaa cgatatatac agtaaggaaa gcactctatc caacaaacca cccacagtta
     1921 atcggaataa aagcagagtg cttatgaatg atcgcacgcc cgaaggttag tatttctgca
     1981 cagcaatttt gcaaaaaaag gcgatcattc ataacttaaa cgtctttcag tcactccggg
     2041 atttcccatc atcgcagact gaaagactct g
//
LOCUS       DAZSNM010000369         2060 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZSNM010000369 DAZSNM010000000
VERSION     DAZSNM010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.373"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..711
                     /locus_tag="ACHW42_004264"
     CDS             <1..711
                     /locus_tag="ACHW42_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR8945814.1"
                     /translation="SSAFPTYAADDVKVTQNTSVFKLKLSATRLIYEQGSDGAAITVT
                     NPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKMWVVQTAPALQDREKLHW
                     LCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLITRPSGIEGKMVNASEKL
                     SWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFSQHEFVMPKTSGQDIKWS
                     VITDEGGESRLFISHLQS"
     gene            785..2060
                     /locus_tag="ACHW42_004265"
                     /pseudo
     CDS             785..2060
                     /locus_tag="ACHW42_004265"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          596 a          377 c          506 g          581 t
ORIGIN      
        1 tcaagcgcat ttcccaccta tgctgcggat gatgtcaaag tgactcaaaa cacttcagtt
       61 tttaaactga agcttagtgc aacgcgatta atctatgaac aagggtcgga tggcgcagcg
      121 ataactgtga caaatccgca ggattggccg atgcttgtgc agagtaaagt ctttgaagaa
      181 gataaagtaa ctgcagcgcc ctttattgtc actccgccac tgttcaggct taatccaggc
      241 cagcaaaata aaatgtgggt tgtgcagact gcgccagctt tacaagacag ggaaaaatta
      301 cactggctat gcgccaccgg cattacgcct aaaaagagtg atgtttgggc cgagaaagat
      361 aaagaaaaaa ttgctgaaaa tgtgaacctg aacatccagg ttaacgtaca tacctgcata
      421 aaacttatta ccaggccatc tggtattgaa ggcaagatgg ttaatgcgag tgagaagtta
      481 tcgtggaaaa agcagggtga taaaattgtc gcggtcaatc agtcgccgtt ctacatcaac
      541 ctttcttctt tacaggttgg cggtagcaag attgagaaac tagattatat tgcgccgttt
      601 tcgcaacatg agtttgttat gccaaaaact agcggccagg atattaaatg gtctgtgatt
      661 actgatgaag gtggtgagag tcgtttgttt atatctcatt tgcaatcata atgtattaca
      721 tttttattga atatttttat gccatgcata ttccatgttt tatcgactgt cattaatttt
      781 gataatggcc ttacttgcgg gtcagttaag tgcccaggag tggtcatttg attcttcaca
      841 gttggaagga aacgttagtg cggacactgt ggcaatgttt aatcagggag agcaactccc
      901 cggaaattat cgtgtggaaa tttatcttaa cggtgaaaaa gttgatgttg gggagtttcc
      961 ctttcatcgt ccggaatccc ctgaagaaaa agagcttgtt ccatgtttaa ccgttgatga
     1021 ccttatccac tatggcatta aaattgataa aagcagcagt gatactgaca ataaaaagaa
     1081 tcagtgtttt aaatggaact ctattgaagg tttgaaagta aattatgatt ttgatagcca
     1141 gcgcgtacag ataaccgttc cacaactgta tctgcaagat aaaaaaagta gtttagcacc
     1201 agtgtcgtta tggaatgagg gtgtagctgc attccgcatg gtgtatcaga caaatattga
     1261 tatttccaaa caaaatgata atcaatctac cacgcgtaat tctcgttatg gcaggttcac
     1321 tccagggttt aatcttggtg cctggaggtt tcgtagttca gtgacatgga gcaaggaact
     1381 tggccagtca gaaagatggc aaaggggtta catgtggttt gagcgaggca taaatgcgat
     1441 taaaagccga ttaacgcttg gagaatctta tacgtcgtct gaggtgtttg acagcatacc
     1501 ttttcgcggc gggatgttag cgaccgacga tgcgatgact ccgccggagg acagctacta
     1561 cacacctgtg gtgcatggaa tagcacaaag tgaggcgcag gttattatta aacaaaatgg
     1621 gcaaattatt tttacccgtt ctgtgccgcc agggcctttt gcgctggata atttaccaac
     1681 gctggccgtt ggcggggagc ttgatgttac agtgagagag agtaatggcg aggagcagta
     1741 tttttccgtg ccatttcaaa cgccggccat cgctctgcat gaaggatatt tcaaatatag
     1801 tgtgatgggg gggaatataa agaagaaggt ataaaaagct cccctgtctc cgtgggcgaa
     1861 atcacggcga tgtacgggct tccttatggc gtcacggtgt atggagggat tcaaaacgcc
     1921 actcatttta atgcaatatc tacgggtatt ggcatttctc tggggctgtt agggagttta
     1981 tcgacagata ttacgcgttc gattgcgaat ttatattatg ggaataaata tcgtattcgt
     2041 tatagtaaat ccattagtga
//
LOCUS       DAZSNM010000370         2040 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZSNM010000370 DAZSNM010000000
VERSION     DAZSNM010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.374"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(13..901)
                     /locus_tag="ACHW42_004266"
                     /pseudo
     CDS             complement(13..901)
                     /locus_tag="ACHW42_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          551 a          475 c          474 g          540 t
ORIGIN      
        1 gtattaagac tatcacttaa ataagtgata ctggttgtct ggagattcag ggggccagtc
       61 tagcagctgc tctacggtca tatttttaat tgcgctccgg tttacaagag cccacccctg
      121 tttttccaga taaaactgga aagtatccag ggtacagacc agtgcaccgt caggaacagt
      181 ttcggtgaat ctgatattgc cgtgttcgtc gaagtgcaca accagagtgc gaccgttacc
      241 cggaatcatc ttgtcagcgg acggggtatt attctggtgc agttctgcct ccatgcggtc
      301 gaacatcagc gatgtaggcc tctttgaatg cagcggcttt tttcccagtg aagcccatca
      361 ccaggaaaac gaagccgttt ttggtgattt ggtacattgg gcgtttttcg cctttggtgt
      421 cggtgtaggt gacgtcctca aaattgaggg cgttaaattc aggtgagcat tcagtatttg
      481 cgatggcacg taacacattg tcgtgtcgtt tgtggaagaa ctctgcaacc gcaacagacg
      541 tagtgacagc gcgaccgttt tcgatggtta cgtcagggtg agaaagggca gggatagtag
      601 ccatgatggc atcctcgagt gataagttaa ttaactcacc accgaggttt tccacgacca
      661 taagggtggt gagacgtaca ggggtggaaa taccggtcac tcgagaaccc ggccagtctt
      721 gcgactgccc tgcacatccc accataattt gaatggagct gtgcattacg cataaaaaaa
      781 ccgcttcagc gcggctatgc gctcgagtaa cgttcgggtt tccacgcccg gcccccgttt
      841 tatgaggtgc agatgcacta taattccacc cgttctggtt ttcaatagct acattcaaca
      901 ttttctctta cctttcatca ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag
      961 gtgacgttaa ccagttaaca attaacgccg gatacaggga aacccataac cacccgatac
     1021 gtgggaacta atcgcttttt aactgttccg cgtgcgctca gccgcattca ccgcatcaca
     1081 aaattcactt taaaaagggc ggacatcagc cagcaatgaa actgatgccg ccaaaggtac
     1141 caatcaacat ggagtgttgt ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc
     1201 cggtgtcctc aacggggaag gaataactcc gccatactta ccgccgcgtc atttcgcgtt
     1261 gtgtgcctgc ttttaactac gtcaggcgag gtggttcctg ttattcccca acaacaagaa
     1321 atttgtataa tctgaatacc ccaacaataa gaagagtgag caagtgatgt attagcgatg
     1381 tcagctctca aagagactgc atcaatgtta aaattattca atgaagcaaa aactcaggct
     1441 gaggttgatc gcgctgtgtc tgaaataaac tctaagttgt caagcattca gcttgaatat
     1501 ttgtcatctc attgatgagc tgacaatctc taaaagcaat gaggctgcgc tcaaagcaaa
     1561 actcacggag atagaaaagt ttaagttcca aagtgaagac tgcgaacctc gctaattgat
     1621 tactggaacg attgtgtatg ccagacagtg caatactagc tctggcaaga ttaccatgta
     1681 tttttgtcca gaatgttttg cgaagagagc aatatctata ttgcaacctt tccctatcga
     1741 ggaaggcgat aaatatacga aaactcaatg cccctcctgt aaaaatgagt atctcatgga
     1801 ctacaatttc aataatgatt tttgagtcac ggtattcaga tttttaaaga acatgccgga
     1861 tactcacccg tgtccggcgc acgcactcca cctcacccgt ggagaactcc ttaattacca
     1921 accttggctt cgttggttag ccattaacgc tggtatgtaa tcattctggc aatgcttaat
     1981 ttgaagtggc acactgaatt tggccacctg aacagaggtg atatgctcac ctcagaacaa
//
LOCUS       DAZSNM010000371         2028 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZSNM010000371 DAZSNM010000000
VERSION     DAZSNM010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.376"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1823)
                     /locus_tag="ACHW42_004267"
     CDS             complement(<1..1823)
                     /locus_tag="ACHW42_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFR8945815.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1931..>2028)
                     /locus_tag="ACHW42_004268"
     CDS             complement(1931..>2028)
                     /locus_tag="ACHW42_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8945816.1"
                     /translation="WNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          486 a          579 c          428 g          535 t
ORIGIN      
        1 cgcagttctt cacttgctga ggaaaaaaca agcgttccat ccttctcgac atacccatcc
       61 gtttcaagca gaccacacag catgcctata cgcgttgatt tatttgcgga aaaaaacaca
      121 cgagggatga atttgttttt tgctgtgcac cccattagtc cataacctcg cagggtctcc
      181 atgagtggat ttacctgacc acgcgccgtt gatatcagcc agtccttacc atctccagag
      241 aagttgtaat caggcagttc agccttcata cgctcgatca tgtatggctc gacgtttgaa
      301 aacttcacgc tcccattgct aagactacca tccccgagaa gggagccgat aacccatgct
      361 aatggctctg agtggtcgcc aaagtcacca gttatttcag gaatcctaac gccatttttg
      421 tgcctcttcg tttcactcaa ccgcttcaac ccgaaggtat ctatcacgcg gcgtttctcg
      481 cctttcgtga atccacgact agtgacctcc cataggtgat cgcctgcgca atcaacataa
      541 cgaccatctt caaatgtgac tcggtaaaca tctctaacac cctgcgggaa tatcccagtg
      601 acagttgacg gattgccgtc tactgacgcc acctgatcgc caatttttac attcccgtga
      661 gtagtccatg tcccatcagc taaaagaata ggctcattca gtgggcatgc ctttccgtga
      721 ccgtgcacac cattgaccag cgtcaactct gcctcacgga actggaattt atctgccaga
      781 gattcccacg gtggattaaa cagatactgc tgcttgccgt agaaagcgtt gatagtgtcc
      841 tggtaaaact ctcgcgcact gtagagttct tcaggatcga agtaggatgc cgtgccgatg
      901 tactgccaga tttcatcctc ggtaacaccg ttcatcaggc attcgttgat gtctttgtac
      961 ggcagagtaa caagacggca acgatgttca ccgagtcggc ttacgatttc ccttgcggct
     1021 tcacgaccaa catcatcaac gtccatcgag atgaatattt cctcaaacct gtcgaggttg
     1081 tgatactcaa actcaatcca ctgttgctta gcgcctttcc cgccaccaaa cggcacggat
     1141 aacgccgaga tgccgtattg cgcatagctc atacaatcaa tttcgccttc gcaaagcaca
     1201 accaccctca cgccagcgtc cagagcctgc catccgaaca ggcaaggttc gcaatcacct
     1261 tctgccataa tgactttctt cccgtccggg cgctcagtgc tgattcgctt gacctgcaac
     1321 aactcaccat cgcgtttgta cggaagcacc agagcatcca gttctcgttc tccattccac
     1381 accttgccgc tgacaacctc gtagcgcttt acgacttctg gcgatatgcc acgcgattgc
     1441 aagtactcaa gatgggattc tgttctggta acgtagcggg cgattttctt acgatcaggt
     1501 ctggagaatt tcttctcacg tctggcatcg aaatggtgat cgtcatcctt gataccgaga
     1561 aatgctttcg cttcctgcat agcctgatgc aggttaattc cacgacatgc catccacaaa
     1621 tcaagcatgt caccgccgtc tccctcagcg aaatcagccc attttttctt gccgataagg
     1681 ttgaccttaa ggctgtttcc cttgtcaccg ttgacgttac cggcaaccca ctcatgcccc
     1741 tctatcttgc cgtttggcaa caggtgcgga gccaccctgt caacctgcgc ccaaagcagg
     1801 tcgctaagtt cacttggcct catgattccc tcagattgag atttttaaac cagaaatcga
     1861 caaacgaaat acttaaccag ccgtggttat aaccagcgac cagtagcgat ttgatttttg
     1921 atttcatggt tcacctgtcg aaaaacacat agccagtttt cgatacggtg attgcggatg
     1981 atggtctgga ttgtggttga atggtttctg gcttctcgtc gttccagc
//
LOCUS       DAZSNM010000372         2018 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZSNM010000372 DAZSNM010000000
VERSION     DAZSNM010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.377"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            205..442
                     /locus_tag="ACHW42_004269"
                     /pseudo
     CDS             205..442
                     /locus_tag="ACHW42_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            556..814
                     /locus_tag="ACHW42_004270"
                     /pseudo
     CDS             556..814
                     /locus_tag="ACHW42_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            863..1441
                     /locus_tag="ACHW42_004271"
     CDS             863..1441
                     /locus_tag="ACHW42_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945817.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1550..1808)
                     /locus_tag="ACHW42_004272"
                     /pseudo
     CDS             complement(1550..1808)
                     /locus_tag="ACHW42_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1792..2018
                     /locus_tag="ACHW42_004273"
                     /pseudo
     CDS             1792..2018
                     /locus_tag="ACHW42_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          648 a          354 c          477 g          539 t
ORIGIN      
        1 atacggtggt gcggctgaga gtcgcagagg ggaagttgcg tccatgttga ggggaacgga
       61 gcaaaaaacc atcagagata cattgaatca atcaatgtaa gcaatattaa gtgtctatac
      121 cgattacttg atgatcaggt tgtgttgaat aaacgcgcat tcttcggtgc ttttttaatc
      181 ttttggggat ttaaattcct atcgatgaac tgtcgatatg aaaaagcgag catcatactg
      241 acgtcaaaca aaggggtcgc agactgggga gaaatgttcg gagatcacgt gctggcaaca
      301 gcgatcctag atcggctgct acacgctgaa tatcaaagga gagagttacc ggttaaaaga
      361 gaaacgtaaa gctggagtgc tgaccaaaaa cacaacgcca atcagtgatg atgaaatggt
      421 ggaaaacgga cagcatcagt aacgaaagta tcttagcagg catgaaaatc gtccgcatcc
      481 cctaaacggt caaaatgctt ctgccgtgaa ggaaggagaa tgccatctca tcacttatat
      541 ctggcgaatg aaacacagat ggtcggcctt gcccccaaac acacggcggt gcaaaatgga
      601 atggcagaaa acttcgtcaa aacgatgaaa tatgattaaa tcagtgttat gttcgaacct
      661 gacgtgttaa cagcagtaaa gtagtctcgc gaaggtgttc aaacattata atgaatggca
      721 tcctcatagt gcgcagggtt atcgcttgct gcggaaatat ctgaagcgac gtaccagtaa
      781 tggggaaaat gatagctggt ttctgaaaat atagaggaaa atgtgatata tatatcctgt
      841 taatatttaa ggagaatata aaatgaaagt ctcgtttaag tctttaggct atatttttca
      901 tgatatatat aataaaaaac acactatcga tgagttcaac gatgtggtaa gaaaagcagt
      961 tttatcaggt aaaattaacg agctgaatgc atgtcataaa gtggcaattt ttcttgccga
     1021 aaaagacaat gagataacaa agaaagacaa agccaagata atagatacat taactgaaaa
     1081 ctattccatt gaatttcaac aattaatgaa tattagtgag cgcacgttaa attcatcttt
     1141 atatattact cctggtgaaa gtggttttgt cagttttgtt aatcgtgaag gaaaaatatg
     1201 tcatactgca tatgtaaaat catcagataa tagcatgaca tattaccatg caaatggtag
     1261 cagcattgac aaatatatta cggatatgtg cggccttatt tgtatgaggc atattgagtc
     1321 tactggcatt attttttata tgcttgatga aaaagtttta tctgccattg ctgaatttat
     1381 gaatgaaaaa ggatggcggg ctgcgttttg ttcagcaaag aacttatata aatgtgttta
     1441 attttagaaa aactatttgg taaaatccaa caagagtcga gaggttcgcg ttgcttcagc
     1501 agggcctcag cctgttcaac cagactcagt tgcaggtcgt gacgcagatt tagtggtggt
     1561 ggttgattgt cagataccag ccgatgatct tgtagtgcat atcatcagac tttgagaagc
     1621 atatggtttt gcgggtaagc cgcttaagat gtgtccgcag gttcagatta tgtcgctcta
     1681 tccgctgagt gcatttcttg cttataacat gactggttga ggacaataaa gttcggtata
     1741 ccggctcagg catcggtcat gtagaaggca atgctaaact ggctcagcaa actaagagca
     1801 gcgcgcatcc acttacccaa tgcggttgag aaaatagcct tcgatcgctt tcatgtggcg
     1861 aaacaaccgg gtgaggtggt ggataaaacc cgccagaatg aacatccgca tcttcctgtt
     1921 gaaagtcgtc gtcaggcaaa gggaactcgc ttcctgtggc agcacagcga taaatggatg
     1981 accgaatcca ggcaggaaaa gctgatatgg ctgcgtgc
//
LOCUS       DAZSNM010000373         2009 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZSNM010000373 DAZSNM010000000
VERSION     DAZSNM010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.378"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            274..549
                     /locus_tag="ACHW42_004274"
     CDS             274..549
                     /locus_tag="ACHW42_004274"
                     /inference="COORDINATES: protein motif:HMM:NF018642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFR8945818.1"
                     /translation="MRDVQWFCPLTPVHTGTEIKPVEMPVTTITPVSDAGGLQDFIYW
                     RPDADRAGMEPVYVILNDPLDSGRFTRKQLNKNILNMQVILVFSIQE"
     gene            747..>2009
                     /locus_tag="ACHW42_004275"
     CDS             747..>2009
                     /locus_tag="ACHW42_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8945819.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYL"
BASE COUNT          523 a          397 c          460 g          629 t
ORIGIN      
        1 gggttgggga gcaatggaac agtaaacgcc gttaagaagt caatttaaat agccaaaaat
       61 taactagcca atttacattg caggttgtaa tttttaacca ccttttttac gatagccaaa
      121 ttacctatac ccatcccggc cctgccaagc catacccgct atcggcgatg aaaatgcttt
      181 tgtgcgcggt cggagtggtc ccgacgaggg ttttacccga agtcggggcg tatctccgcg
      241 ttagcgggcc gtgagggccg cttacgagcg tgtatgagag acgtacagtg gttttgtccg
      301 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
      361 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      421 agggctggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      481 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      541 caagaataaa tagtagtaac tactatagct gcaaataatg ttaaaaacat ttctatgatc
      601 atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      661 tgataatgca atactttgag tgattatata atgataagta aacaggtttg cgacaattgg
      721 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      781 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      841 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      901 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
      961 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
     1021 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
     1081 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
     1141 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
     1201 cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
     1261 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta
     1321 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1381 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc
     1441 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1501 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1561 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1621 aggatatggg aaaactggca tcaatggggg ggggttctgt ttttgaaatc ggtggtgtgg
     1681 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1741 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1801 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1861 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1921 gagccgcatc agggctgtca ggcacatttt cctatatctt cggctcaatt gttgcgacac
     1981 tgggaatggg acttgttgtc gattatctg
//
LOCUS       DAZSNM010000374         2003 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZSNM010000374 DAZSNM010000000
VERSION     DAZSNM010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.379"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..414
                     /gene="torD"
                     /locus_tag="ACHW42_004276"
     CDS             <1..414
                     /gene="torD"
                     /locus_tag="ACHW42_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR8945820.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACHW42_004277"
     CDS             complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACHW42_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR8945821.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACHW42_004278"
     CDS             complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACHW42_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR8945822.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT          479 a          509 c          508 g          507 t
ORIGIN      
        1 acgctgacag tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt
       61 ctgatgaccg acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa
      121 gagattaaac gcttgttagt tgaggcaggg atggaaacca gtggcaattt caacgaaccg
      181 gcagatcatc tggcgatcta tctcgagttg ttgagttatc tgcatttttc gctgggagag
      241 gggaccgttc ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgcgg
      301 caatggttac cagagtttgc tgcgcgttgt cgacagtatg acagctttgg tttttacgcg
      361 gcactaagcc agttattgct ggtgttagtg gagtgcgacc accaaaacag ataacgtcgt
      421 ttatgcgcct gaaaagacgc gtttagcgtc gcatcaggca ttatggcgca gttgccggat
      481 gcgtcatcac atccggcaaa cacacatcac ggatgagcta caaaccggga aagccgctgg
      541 cgtagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc
      601 gcgatccccg gccagtccag agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca
      661 atggcggcat ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc
      721 cccaaaccga caatttcatt caactcctct tcagagatgc cggtatgcag gcaaaattcg
      781 gtaatagtaa aagtcaccgt aacattagcc attatgcttt cccccaatct ttacgtggat
      841 caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt
      901 tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca
      961 gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gcctggcggg atagtcagca
     1021 aaatgctttc tttcagtgtt ggaacggtga ctttagtacc cagcgccgct tcccacgggc
     1081 taaccggcac cacaatttcc agatcctggc cgacaatatc gaacagcgga tgtggcgcaa
     1141 tatgaatcac cagccacaaa tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc
     1201 ctttcaggcg gatgcgttga ccattgccga cgcctgccgg gatcttcaca ttcagcgttt
     1261 tcgggatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg
     1321 tacgcttatg ctcagtaagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt
     1381 ggccacgtgc ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga
     1441 agatatcgtc aaaatcttcg gcattaaaac tctgaccgtc gccatgatgg aactgacggt
     1501 taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat
     1561 cacttaacac ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt
     1621 tgctgacatc aggatggtat ttacgggcca gtcgacgata ggcggtcttg attgtcttga
     1681 gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat
     1741 ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg
     1801 taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga
     1861 ggtcgccatc aagatgggtt gctgaacata ttttaaacag gtgaaaaagg gtgagcgatt
     1921 tttgatagtt gaaccaggca ctttaagttt aactagggcg tcattattta ttaaatttta
     1981 tagacgctat atatgggtag taa
//
LOCUS       DAZSNM010000375         1994 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZSNM010000375 DAZSNM010000000
VERSION     DAZSNM010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.380"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..114)
                     /locus_tag="ACHW42_004279"
     CDS             complement(<1..114)
                     /locus_tag="ACHW42_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR8945823.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFT"
     gene            complement(170..787)
                     /gene="yeaW"
                     /locus_tag="ACHW42_004280"
                     /pseudo
     CDS             complement(170..787)
                     /gene="yeaW"
                     /locus_tag="ACHW42_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
     gene            complement(1129..1737)
                     /locus_tag="ACHW42_004281"
     CDS             complement(1129..1737)
                     /locus_tag="ACHW42_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8945824.1"
                     /translation="MTGEKESKNAERIFMPPEIMRFKERLEEAMNGESSRAFAAKCGL
                     SDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDR
                     STLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQ
                     TAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            1907..>1994
                     /locus_tag="ACHW42_004282"
     CDS             1907..>1994
                     /locus_tag="ACHW42_004282"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8945825.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT          535 a          445 c          444 g          570 t
ORIGIN      
        1 ggtgaacgca ggcagcggtt tgccatcggt tgccaccagc gtgaagcgtt tcacctgttc
       61 ggtaaggggt tcaacctggc tcacctgtac ttcaaacatt tgatagtctg acattgctgt
      121 actccatcaa aaatcagccc agcggcggaa aataccgccg ccgatgtcat tagtccttaa
      181 acacctgcgc cagcagatta tggaaatggg cgataccatg ttcggaaatg ccactaccgc
      241 tactgtcggc catgatgcgc ccctgaccac gatagccacg cgatttcagc cctttctgta
      301 cgctttcaac cagacgtaaa tcttccggat ggaacacatc gcgataccac tcaatcagcg
      361 atttttgctc gtcggttaac tcttcattgg tgaagtaaat atcgtagttt tgcagggtag
      421 tttcagaatc caccgggaat tcataaatga ccgtcatcat ccctttgatc ggggtgacgt
      481 tcagcatcgt gcacggccac agccagaaac cgtggaatgc cgcatccgta ccctcttcaa
      541 atttaaacga ctgttcggac ggtttggcga aaccgtattg cagcgtccag ttaccgtgca
      601 tggtgtgcca gtaacgatca acctgtacgg agtcggagaa acctagatgc gccggaccac
      661 agtgatagca ctcgagatag ttatcgacaa tgttcttcca gttggcaggc gtgcgggtgg
      721 taaagcgggc cgctagtttc agattgtgga cttccgggca ggcttccagc actttcgcgc
      781 ccagaccggt gtattgattc acttgaagta cgaaaaaaaa ccgggaggac attggattat
      841 tcgggttctg atgggattag atttggtggg gcttgcaagc ctgtagcgca aattttagtc
      901 gttaatcaat gaaacgcgaa agatagtaaa aaattgcttt tgtttcattg aaaatacgaa
      961 aaacaaaaac actgcaaatc atttcaataa cagcttcaaa aaacgttcaa aaccgataac
     1021 aaccaggctg taaccaaatg actcatatca caaatcagct catgccgttt aggtatgtta
     1081 catgtgtgat tatgtgaggt gaagtatgtt ttagctggtt catggttgtt atacggcttt
     1141 ttttacatct tgtggttctt gtgaaggagc tacaagacct tcctgttcat aaataccata
     1201 ctttgacaaa atctttttac gagtatctgt tggtaatgat tcaagcattt tggctgtttg
     1261 taaggtttgc tgcgaaatac cttgcaaatc agcctggatg gaatcatcgg aagaaaaaat
     1321 ctcaggcata agagcttcaa atcctttctt tctgaacaac tcaatcgcag aatgcagttc
     1381 cttgtcctct aacaattcga caattgcgat cagtttagtt ttcaatgtgc tgcggtcagg
     1441 ttgagaattt ttattctctt gattattttt atgctcaacc attggcatgc tctgactttg
     1501 gataaaccac tctattggac gccctgttgc gtaggctatt tgtgctaatc gatctaaaga
     1561 cgggtatgtc ttgccagaca ggtagtttct gataacccca tcagaaagtc cgcacttagc
     1621 tgcaaacgcc cggcttgatt ctccattcat tgcctcttca aggcgttcct tgaaacgcat
     1681 tatttccggg ggcataaaaa tgcgctctgc atttttgctt tctttttcac ctgtcatgcc
     1741 caatctctct gttatataaa gaattttaca gaattctttg atttgcactg gttaaaataa
     1801 aaatgcgcat agcttttgaa ttgagcattt ttctgcgcta tcgtttttcc cgatagcggt
     1861 agccggtatc agttaccgaa aacgttaaga gagtaaggat cgcagaatgt cagcaaaaaa
     1921 ccaagactgg cacgcagagc agataaaggc tgctgttcgt atgaagggga agacactatc
     1981 tcaattatcc agag
//
LOCUS       DAZSNM010000376         1992 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZSNM010000376 DAZSNM010000000
VERSION     DAZSNM010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.381"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            111..635
                     /gene="aroL"
                     /locus_tag="ACHW42_004283"
     CDS             111..635
                     /gene="aroL"
                     /locus_tag="ACHW42_004283"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR8945826.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            685..876
                     /gene="yaiA"
                     /locus_tag="ACHW42_004284"
     CDS             685..876
                     /gene="yaiA"
                     /locus_tag="ACHW42_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR8945827.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            1134..1811
                     /locus_tag="ACHW42_004285"
     CDS             1134..1811
                     /locus_tag="ACHW42_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR8945828.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            1883..>1992
                     /locus_tag="ACHW42_004286"
     CDS             1883..>1992
                     /locus_tag="ACHW42_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR8945829.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYT"
BASE COUNT          539 a          435 c          503 g          515 t
ORIGIN      
        1 ttaagtggaa ttttttcttt acaatcgaaa ttgtactagt ttgatggtat gatcgctatt
       61 ctcatgacac cggctttcgc cgcattgcga cctattgggg aaaacccacg atgacacaac
      121 ctctttttct gatcgggcct cggggctgtg gtaaaacaac ggtcggaatg gcccttgccg
      181 attcgcttaa ccgtcggttt gtcgataccg atcagtggtt gcaatcacag ctcaatatga
      241 cggtcgcgga gatcgtcgaa agggaagagt gggcgggatt tcgcgccaga gaaacggcgg
      301 cgctggaagc ggtaactgcg gcatccaccg ttatcgctac aggcggcggc attattctga
      361 cggaatttaa tcgtcacttc atgcaaaata acgggatcgt ggtttatttg tgtgcgccag
      421 tatcagtcct ggttaaccga ctgcaagctg caccggaaga agatttacgg ccaaccttaa
      481 cgggaaaacc gctgagcgaa gaagttcagg aagtgctgga agaacgcgat gcgctatatc
      541 gcgaagttgc gcatattatc atcgacgcaa caaacgaacc cagccaggtg atttctgaaa
      601 ttcgtagcgc cctggcacag acgatcaatt gttgatttta gagcgcctat acttaacgtt
      661 catcccgtga aataaggaag aacgatgcca acgaaaccgc cttatcctcg tgaagcatat
      721 atagtgacga ttgaaaaagg aaagccagga cagacggtaa cctggtacca actcagagcc
      781 gatcatccta aaccagactc gttgatcagt gaacatccga cggctcagga agcgatggat
      841 gcgaaaaaac gctatgagga ccctgacaaa gagtgaccgc atcagactgc tcggaaggga
      901 ttctgagtgc cactacaagg gatctgcgtc acatttttca taatttatgt ttttctaata
      961 attagaatat taaacaataa cattccatta ctggaatcat ttggaatctt tacattatgc
     1021 cgtgcacgtc tgctgctacg ctttttgtca tttgtagcac aagtaagtgt cagcagtggt
     1081 gcttcacact tgcccggtaa ttaacgacga aagaaaagta aggtggatga acaatgagtg
     1141 cgtcgttggc gatcctgacc atcggcattg tacctatgca ggaggttttg ccgctcctga
     1201 cggaatacat tgacgaagat aatatttccc atcatagcct gctggggaag ttaagtcgtg
     1261 aagaagtgat ggcggagtac gcgccagaag caggcgaaga caccattctc acattattaa
     1321 atgacaacca gctggcccat gtttcgcgtc gtaaagtgga gcgagatctg caaggtgtgg
     1381 ttgaagtgct cgataatcag ggttatgacg tcattttatt aatgagtaca gcaaacatta
     1441 gtagtatgac tgcgcgtaat acgatctttc ttgagccgtc gcgaatattg cctccactgg
     1501 tttcctctat tgttgaagat catcaggtgg gggttatcgt tccggttgag gagatgctgc
     1561 ccgttcaggc gcaaaaatgg caaattttgc agaaatcgcc ggtattttca ttgggtaacc
     1621 ccattcatga ttcagaacaa aaaatcattg atgctgggaa agaattactg gcaaaagggg
     1681 ctgatgtcat catgctggat tgtttaggat ttcaccaacg tcatcgcgat ttactgcaaa
     1741 aacagctcga tgttcctgtc ttgctctcta acgtattgat tgcacggctg gctgcggaat
     1801 tactggtgta attttgcgtg acagccagcg cctctggccc ctatagtgaa gtagatgttc
     1861 aactaccaga cagggccagt ttatgcttca aagtaatgag tacttttccg gcaaagtgaa
     1921 atcgatcggc ttttccagca gcagcactgg tcgcgccagc gtgggtgtta tggttgaagg
     1981 cgaatacacc tt
//
LOCUS       DAZSNM010000377         1966 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZSNM010000377 DAZSNM010000000
VERSION     DAZSNM010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.382"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(53..913)
                     /locus_tag="ACHW42_004287"
     CDS             complement(53..913)
                     /locus_tag="ACHW42_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR8945830.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1096..1527)
                     /locus_tag="ACHW42_004288"
                     /pseudo
     CDS             complement(1096..1527)
                     /locus_tag="ACHW42_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1593..>1966
                     /locus_tag="ACHW42_004289"
     CDS             1593..>1966
                     /locus_tag="ACHW42_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR8945831.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILN"
BASE COUNT          483 a          514 c          453 g          516 t
ORIGIN      
        1 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
       61 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
      121 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
      181 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
      241 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
      301 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
      361 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
      421 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
      481 ccttcggtcc tccgatcgtt gtcagaagta agttggcagc agtgttatca ctcatggtta
      541 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
      601 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
      661 cggcgtcaac acgggataat accgcaccac atagcagaac tttaaaagtg ctcatcattg
      721 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
      781 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
      841 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacgaaaat
      901 gttgaatact catactcttc ctttttcaat attattgaag catttaccag ggttattgtc
      961 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     1021 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     1081 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attttataaa ccgtggagcg
     1141 ggcaatactg agctgatgag caatttccgt tgcaccagtg cccttctgat gaagcgtcag
     1201 cacgacgttc ctgtccacgg tacgcctgcg gccaaatttg attcctttca gctttgcttc
     1261 ctgtcggccc tcattcgtgc gttctaggat cctccggcgt tcagcctgtg ccacagccga
     1321 caggatggtg accaccattt gccccatatc accgtcggta ctgatcccgt catcaatgaa
     1381 ccggactgcc acgccctgag cgtcaaattc ctttatcagt tggatcatat cggcagtgtc
     1441 gcggccaaga cggtcgagct tcttaaccag aatgacatca ccttcctcca ccttcatcct
     1501 cagcaaatcc agcccttccc ggtctgttgg gcactgttgc aaatagtcgg tggtgataaa
     1561 cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc cggcattttc
     1621 agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc agttaccgtg
     1681 agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg atttaccgct
     1741 gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg cgtaaccctt
     1801 ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc cgctgggcgt
     1861 atctgtaccg ggccgtcgac agccggggcc gcactgtcga tttttatctc tcctcccgtc
     1921 gtaacagcaa agctgcatac cggtttctgg gtaaaatcct caacaa
//
LOCUS       DAZSNM010000378         1935 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZSNM010000378 DAZSNM010000000
VERSION     DAZSNM010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.383"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACHW42_004290"
     CDS             complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACHW42_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR8945832.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(590..1255)
                     /locus_tag="ACHW42_004291"
     CDS             complement(590..1255)
                     /locus_tag="ACHW42_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR8945833.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(1424..1780)
                     /locus_tag="ACHW42_004292"
     CDS             complement(1424..1780)
                     /locus_tag="ACHW42_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945834.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            complement(1838..>1935)
                     /locus_tag="ACHW42_004293"
     CDS             complement(1838..>1935)
                     /locus_tag="ACHW42_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR8945835.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
BASE COUNT          466 a          502 c          430 g          537 t
ORIGIN      
        1 ccatccctct tctccctgcg cgctacttaa gtgagtcgat tctatctgcg aaggtgcgcg
       61 aatcaaattc accggaaata aacaacaaaa aacccgccga aacgctgaga gatcacagcg
      121 atgggcgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt gggcggggat
      181 gggatcagct ccccacccgg tcactcttac ttcctggatt cgtagtctac gaagacagcg
      241 acctccgtct ggccggttcg gattcgtacc tcacagaggt ctttcctcgt taccagtgcc
      301 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      361 atagcctgct tctccttgcc tttcggcatg taagaggcta acctacatgt gcaaagcatg
      421 aaattggcct cagattaatg ttaagcgtct tgccggacgc gtaatgttaa ctggggcttt
      481 tctctatctg ccttttggtg ttcatgcctg aagcagatag cctcaagcac ccgcagtcat
      541 tctacttacc tcgccaatat gaaatcaatc agaaaggtgc cccataaaat cactccttct
      601 cttctttacc gtagtggagt tgaccaattt tgataagagg gcgtccctga gatttgcggt
      661 gtagattggt atcgcgcaga gaatacacac agccacaata ttcctgctga tagaattttt
      721 cgcgcttgct gatttcaatc atacgggacg agccgccctg cttgcgccag ttataatccc
      781 agtacaccat gcccggataa tgcgcgacgg ctcgccgccc acagtcgtta acctgctgca
      841 tatttttcca gcgtgaaatg cccagtgaac tgctgatcac actgaaacca ttttcagcgg
      901 cgtacagcgc tgtccgctca aaacgcatgt caaaacacat ggtgcaacgg atccctcgtt
      961 cgggctccca ttccattcct ttggctcgtt caaaccagtt gtctgtgtcg taatcagcat
     1021 caataaacgg cacgccgtgt tgttcagcaa agcgaatatt ctcatcctta cgaattaaat
     1081 actctttctg aggatgaatg ttcgggttgt agaaaaagat ggtgtagtcg atttccgagg
     1141 cctgaagcgc ctccatcacc tcaccggaac atggagcaca gcaagagtgc agtagtagtt
     1201 tgtttgcccc gtttgggagc tccaatttgg gccgtttgaa atcagcaact gtcataaata
     1261 tgtttattga ggtcataaaa atatcaaaga gtgtagcatt agaacggggc tatcggaaac
     1321 agatgtgtga ctgctccccg ccctttcggg cggtctcctg atgatttgag ggtgcagaaa
     1381 tccctccggt taaggattaa atttttaaca gtgctaaatt taattattca gttctggatt
     1441 ttgtcgccct gcgtatccgc gctttcgcgt tacgctcaat ctgtatcagc ttttctatat
     1501 ttctccgcct ttcctgttcc tcctggcgca atagccttac atcatctgcc agtctggttt
     1561 ctctttttgc cacagagagc atccagtcaa acggctccac aaccgcaccg cagattttac
     1621 agcggacctg acgctctttt tcgtcaaccc gtacagaggc gtgatggcaa tatggtcttt
     1681 ccgatggctt ataaagaaaa ttaacctgat tacgagggtc atcctctttt atcggaaata
     1741 aaacgatatt gcttaactca tcttctggtt ttatttccat gctcctctcc tttgatgcga
     1801 atgccagcgg taattgaagc ctgatagcta atttcattca cagtatcgcc tcctgaaaat
     1861 taccctgata gaaagccagt acacgctgca tagcttcgct cttccggcac tcgcgacaga
     1921 ttatattcag gcgcc
//
LOCUS       DAZSNM010000379         1921 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZSNM010000379 DAZSNM010000000
VERSION     DAZSNM010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.384"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(30..1181)
                     /locus_tag="ACHW42_004294"
                     /pseudo
     CDS             complement(30..1181)
                     /locus_tag="ACHW42_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
     gene            1637..1894
                     /locus_tag="ACHW42_004295"
     CDS             1637..1894
                     /locus_tag="ACHW42_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945836.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
BASE COUNT          478 a          482 c          422 g          539 t
ORIGIN      
        1 gatcctaccc acgtaatgtg gacacggccc taagcgagat tatggttttc aaattgttcc
       61 gggctgagac cgccacaggc actgtgacga cgccagcgat tgtaatcaca ctcgatataa
      121 ttaaacaccg ttgcccgcat tatttcccgg ctgctaaagc gttccccgtg gatacattcc
      181 accttcagcg aatgaaagaa gctttccaca caggcattgt cataacagta gcctttcgca
      241 ctcatactgc cacgcaggtt gtgtcgcttc agcattgcct gataatcccc tgaacagtat
      301 tgaccaccac ggtccgtatg aacaatgacg ctttccgggc gtcttctccg ccacaacgcc
      361 atttgcagtg catcacaggc cagttgtgct gtcattcgcg gtgacatcga ccagccaatg
      421 acggcgcgtg accacaggtc gatgactact gcgagataca accagccctc atcggtacgc
      481 aagtacgtga tgtcacccgc ccacttctgg tttcgggcca ctggcgctga agtcctgctc
      541 cagcagattc tccaatacgg gcaggccatg tgcacggtag ctgaccgggc tgaacttccg
      601 gctggctttc gcccgcagcc cctgacgacg caggctggcg gcaatggttt taatattgaa
      661 ctccggcagt tcgtcagcaa ggcggggagc accgtatcgc tgctttgcct caaagaatgc
      721 cttatggaca gcggcatcgc aggtgagccg aaactgttgg cgcaggctca tctggtgacg
      781 acgcctgagc cagacatacc agccgctgcg ggcaacccga agtacacgac acatcgcttt
      841 gatgctgaac tctgcccgat gattttcgat aaagacatac ttcatttcag gcgcttcgcg
      901 aagtatgtcg cggccttttg gaggatggac agttcctcag cctgctccgc cagttgtcgt
      961 ttaaggcgga cattttcagc ggccagttcg ctttcgcgct ctgacgaact catttgttgc
     1021 tgctgtttac tgcgccaggc ataaagctga gattcataca ggctgagttc acgggctgcg
     1081 gcggccacac cgatgcgttc agcgagtttc agggcttcgt tacgaaattc aggcgtatgt
     1141 tgtttacggg gcttcttgct gattgatact ggttttgtca tgagtcacct ctggttgaga
     1201 gtttactcac ttagtcctgt gtccactatt ggtgggtaag atcaccctcc attaaaacta
     1261 aaacgggcgc tttctgatat atcatctgta agcgcagtaa taccattagt agcacctcgc
     1321 gcattgatta ttgaatatta taactggaat gttatatttc ttgttatgat gctgttcagt
     1381 attataataa tgttattatc accaaagata actcatgcaa atgagaaagt aatatgtcaa
     1441 aacagtgcat cctttgtgac caaagagttt atctgcggca ctatacttgt ctcattaata
     1501 ctcagttctc tattttgcta catatcaata tcaccatcaa ttttcatgca tacttatggc
     1561 ctgagtactt ttagttactc aattattttc tccttctcag ttttattttt cataatcgga
     1621 aatcaattat caaagattga aaaattatcc aacctatggt ttttattacc caatagccaa
     1681 ttttatcgca ttacaaataa atggttcaaa aacaggattt gccgcatcta ttacaggttt
     1741 tactcaaaca ataagcgctg gtacaatcag tttcctgtca gtaaccgtaa gcgacaacgg
     1801 tgttggtccc ggaactactt taggtcttgg ttcactaata acttcattac tgtcaatagc
     1861 cgtaatcgcc attgcaggaa ataataaaaa gtaagtgtac tctgactgcg tatgtatcta
     1921 c
//
LOCUS       DAZSNM010000380         1914 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZSNM010000380 DAZSNM010000000
VERSION     DAZSNM010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.385"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(53..1702)
                     /locus_tag="ACHW42_004296"
     CDS             complement(53..1702)
                     /locus_tag="ACHW42_004296"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR8945837.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          507 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aatc
//
LOCUS       DAZSNM010000381         1885 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZSNM010000381 DAZSNM010000000
VERSION     DAZSNM010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.386"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1017)
                     /locus_tag="ACHW42_004297"
     CDS             complement(<1..1017)
                     /locus_tag="ACHW42_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HFR8945838.1"
                     /translation="MTESSDCESVLVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARNAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKR"
     gene            1268..1741
                     /locus_tag="ACHW42_004298"
     CDS             1268..1741
                     /locus_tag="ACHW42_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8945839.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          477 a          442 c          519 g          447 t
ORIGIN      
        1 ccgtttgaga gctttattac cccgtcgcga gggatgctca ccgcgtattg acgagccgga
       61 tcgccgagtt accggcgcaa ggccagcata agcagcgaga tgtgcggcag aggcgaaggc
      121 gcggcaggcg acctcggtga ggagtctggc tgcggtcctg acaccgactc cgggcatact
      181 ggtcaggacc gggtaaagag ggtgagcaag aactcgctgt tctacctcaa gcgccacctc
      241 gtctctttgc ttacgcagcg tgatgagctg gagtgccaga cgtggcagta ctacggcagc
      301 ggcattcgtg ccgggaacga cgacggtttg ttcggccagt gcctgagcta tgtctgctgc
      361 aaggcgttta cccagacgag gcgcaagttt gcagagctgg gctgccagct tcttctcacc
      421 cagcgaagcg agtttttctg gtgagggata tcgctggaga agatcgagta ccgccgggtg
      481 ctcaagtctc ggaccgagaa cgcgctccgg tgccggatgt atctgggtca gaaggccgcg
      541 gatacggttg ctggcctgcg ttgtctgtgc ggcaagatca tcatcgaagc cgcagagcat
      601 ggagagttcg gcgatttgct cgtcagccag tttcagcgtg cgtagcgcgt gaggcagggt
      661 acgggcagct tcggcaatga tggcagcgtt acgagcatca gttttagctt caccggcgtg
      721 taagtcggct atgcggcgca tggccagtcc agggaggtat ccgacaagga ctccttctga
      781 gcgggcaacg gcgacaggta acgcaccgat ggtagctggc tgatcaacaa ccagcagtat
      841 ctgaccatgt tgtttcaggt cagatattag cgacctgagt ttgttttcgt cgttgggtaa
      901 tgctttatcg aacaggcgtt tacctgaacg attaatggca acagcgtgat gcgtatcttt
      961 accgacatca acgccgataa agaccaggac ggattcgcaa tcgctggatt cggtcattct
     1021 gtctcccttg tatatgggtt aaccagataa ccacggggag caggtaccgg catccacgtt
     1081 acagacggtc ccggcaaaag tgcctgacct gacccctatt agcggttacc agcgccccac
     1141 cagacccggt gacatcaccc cccggatcat ggacgactgg gggcagtaat catgccgggt
     1201 ctggctggct aacaccccat tataaggggt acgaataaag taacgggggg gagatgaacc
     1261 ctatgcaatg tcctggttaa tatctcagtg tgctcatcag tgcactgata ataaaaagac
     1321 ggagacagat gccatttacg ataaagttcg gagttcttat ttgctttcat gtattctgaa
     1381 aaaaaacaaa aatgttgggc ttattcttca tgcaccatca tttgtttctg tgtctgaaaa
     1441 aatcgccaga attgtgatgg caaattactc ccgtaactgg agtaattccg agttggccag
     1501 tgcggtttta atgagtgaat cttctttaaa aagaagaatg tacaaagagg tcggttctat
     1561 aagtacattt gtacataaaa taaagcttac agaagctatc aggaaattac gaaggacaaa
     1621 tactcccatt tctgtcatat catctgagtt aggttacagt tcaccatcgt atttttctaa
     1681 agtcttcttt aaatatttaa agacataccc tcaaaacata aggaagaaaa atggtcggta
     1741 atgtcaccaa ttaggaggac ttgcagagcc gcttttttac gatactgaga tcaaacagac
     1801 ataatctatg cggagattta cagcagggca tggttcacaa atctgacagc gatgaattat
     1861 cggcactgcg ggcagaaaat gctcg
//
LOCUS       DAZSNM010000382         1860 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZSNM010000382 DAZSNM010000000
VERSION     DAZSNM010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.387"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..344)
                     /locus_tag="ACHW42_004299"
                     /pseudo
     CDS             complement(1..344)
                     /locus_tag="ACHW42_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(344..1264)
                     /locus_tag="ACHW42_004300"
     CDS             complement(344..1264)
                     /locus_tag="ACHW42_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR8945840.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1402..>1860
                     /gene="pgrR"
                     /locus_tag="ACHW42_004301"
     CDS             1402..>1860
                     /gene="pgrR"
                     /locus_tag="ACHW42_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR8945841.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVLRCTGNSG"
BASE COUNT          465 a          451 c          433 g          511 t
ORIGIN      
        1 cacacaccca gcacgccaat gcgttcagca tcaacatatg gcagagttac tgcataatcg
       61 ataacacggc taatatcctc tacgcgctgt gtcggatctt ctatccaacg aggctaccca
      121 ccagattctc cctggaaact ggcgtcataa gcgagaacga catagccttt ttcagccaat
      181 gccttgccat agatattgcc agacgtctgc tccttacaac tgccaaaagg atggacgctg
      241 ataatcgccg gatactgttt tgactcatca aaatttggtg gaaactgaat attggcagca
      301 atatgccagg ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa
      361 agaacggagc taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca
      421 tatggtttgc gccttcaaca atgtgatagc gtttatcctg gcttgatgcc cgatcgtaca
      481 ggtcgtcact catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag
      541 tcaggtacac ttccgccata tggtaagcat cataggtaat aatctggtta aggctgcgca
      601 aagtagcgta acccggtgct gttggatact gcgcacgagg tgtgtggtaa tactcccagg
      661 cctgacgcag ttcttcgttc ggcgcatcgg cctccttcat tggagccagt ggcataatgg
      721 cgtattcttc gctgctaata tcgctggttc gggcgtttga gccagcttca atgtgtggca
      781 gagcatcaat agatttcaca ttgttttccc aaccattgcg gaacatcgaa ccaatattga
      841 ccgcactaac ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat
      901 atcctgcacc ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg
      961 ttgtcaggta atcaatcacg gcactgacgt cttcagtacg aatgtatggg ttttctaact
     1021 gacgcggctc gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc
     1081 ccttttccgc cagcttttcg gcataggttc cggccgtttg ctctttaacg cccccacctg
     1141 ggtgagatac cacaattgcc ggatactgac gggtttcatc aaattttgga gggaaataga
     1201 tcactgcaga caaagagatg gttggattat tgctgttagt gaagctgact ttattattca
     1261 tcattcagtt cctcatggag ttgtcggttc gttttgacgg ttggtgatat cactatagat
     1321 attgatcatt aagttgatta gaccctaaat catgattaga cctatcattt aaatgattaa
     1381 tagattgtat ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc
     1441 gtcgttgcag aggagcgtag cttcactcgc gcagcagccc gcctgagcat ggtgcagtca
     1501 gctttaagcc agatagtgcg ccgtatagaa gaacgattgg gattgcggct tctgacgcga
     1561 accacgcgca gcgttgtacc cactgaagcg ggcgagcatc ttttgtctgt tcttggccct
     1621 atattgcatg acatagattc tgccctgaca tccctgagcg atctgcagaa ccgcccatcc
     1681 gggacaatac gtattactac tgtagaacat gcagcaaaaa cgatattgtt accagccatg
     1741 cgcacattcc tgaaatcgca tcctgaaatt gatattcagc ttaccattga ttatggtttg
     1801 accgatgtcg tttctgaacg ttttgatgca ggcgtcctga ggtgtactgg caatagcgga
//
LOCUS       DAZSNM010000383         1855 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZSNM010000383 DAZSNM010000000
VERSION     DAZSNM010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.388"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="ACHW42_004302"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="ACHW42_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR8945842.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..>1855
                     /gene="mcbR"
                     /locus_tag="ACHW42_004303"
     CDS             1351..>1855
                     /gene="mcbR"
                     /locus_tag="ACHW42_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR8945843.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHY"
BASE COUNT          489 a          394 c          519 g          453 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatc
//
LOCUS       DAZSNM010000384         1849 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZSNM010000384 DAZSNM010000000
VERSION     DAZSNM010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.389"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..257)
                     /gene="ydfV"
                     /locus_tag="ACHW42_004304"
     CDS             complement(<1..257)
                     /gene="ydfV"
                     /locus_tag="ACHW42_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFR8945844.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            282..521
                     /gene="relB"
                     /locus_tag="ACHW42_004305"
     CDS             282..521
                     /gene="relB"
                     /locus_tag="ACHW42_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFR8945845.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            521..808
                     /gene="relE"
                     /locus_tag="ACHW42_004306"
     CDS             521..808
                     /gene="relE"
                     /locus_tag="ACHW42_004306"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFR8945846.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            880..1035
                     /gene="hokD"
                     /locus_tag="ACHW42_004307"
     CDS             880..1035
                     /gene="hokD"
                     /locus_tag="ACHW42_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR8945847.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1253..1504
                     /gene="rem"
                     /locus_tag="ACHW42_004308"
     CDS             1253..1504
                     /gene="rem"
                     /locus_tag="ACHW42_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFR8945848.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1571..1849
                     /locus_tag="ACHW42_004309"
                     /pseudo
     CDS             1571..1849
                     /locus_tag="ACHW42_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          507 a          410 c          467 g          465 t
ORIGIN      
        1 gacatgctaa agcaaaaaat agatgaataa gttgagttgt gcatatgtag cctgaccgtc
       61 acaaagtata tggtgtctgt accagtaaga tgatggccgg actctttaaa aaagagctga
      121 cctgcacaat acaggatgga cttagcaatg gctgctcctg gcacaaagcg gacagtgatc
      181 accgttctta cgactacttt ctgacttcct tcgtgacttg ccctaagcat gttgtagtgc
      241 gatacttgta atgacatttg taattacaag aggtgtaaga catgggtagc attaacctgc
      301 gtattgacga tgaacttaaa gcgcgttctt acgccgcgct tgaaaaaatg ggtgtaactc
      361 cttctgaagc gcttcgtctc atgctcgagt atatcgctga caatgaacgc ttgccgttca
      421 aacagacact cctgagtgat gaagatgctg aacttgtgga gatagtgaaa gaacggcttc
      481 gtaatcctaa gccagtacgt gtgacgctgg atgaactctg atggcgtatt ttctggattt
      541 tgacgagcgg gcactaaagg aatggcgaaa gctgggctcg acggtacgtg aacagttgaa
      601 aaagaagctg gttgaagtac ttgagtcacc ccggattgaa gcaaacaagc tccgtggtat
      661 gcctgattgt tacaagatta agctccggtc ttcaggctat cgccttgtat accaggttat
      721 agacgagaaa gttgtcgttt tcgtgatttc tgttgggaaa agagaacgct cggaagtata
      781 tagcgaggcg gtcaaacgca ttctctgaac caaagcatga catctctgtt tcgcaccgaa
      841 ggtgacactt ctgctttgcg ttgacaggag aagcaggcta tgaagcagca aaaggcgatg
      901 ttaatcgccc tgatcgtcat ctgtttaacc gtcatagtga cggcactggt aacgaggaaa
      961 gacctctgcg aggtacgaat ccgaaccggc cagacggagg tcgctgtctt cacagcttac
     1021 gaacctgagg agtaagagac ccggcgagtg agaaatccct cgccacctct gatgtggcag
     1081 gcatcctcaa cgcacccgca cttaacccgc ttcggcggtt ttttgttttt attttcaacg
     1141 catttgaagt tctggatggt gccggaatag aatcaaaaat acttaagtag cgcgcaggga
     1201 taaaagggat ggaccccgaa caggggagtg ctacttatct ggaaggattc tgatgatgaa
     1261 catcgaagaa ctgcgtaaaa ttttttgtga agatggcctc tatgctgtgt gcgttgaaaa
     1321 tggaaatatt gttagtcatt accgcattgt gtgtttgcaa aaaaatgggg ctgcgttaat
     1381 taattttgtg gatgcccgag tgacggacgg atttatcttg cgcgacggtg agtttgtcac
     1441 ttcattacag gcattgaaag agatcggaat aaaagctggc ttttctgctt tttcagaaga
     1501 ataaactcat ctacaatctt gcgcggggct gaactcccgc tgagtaacac cgtgccaccg
     1561 gagaaaaccg atggcacgca acgcaaaata ttacaatcat gataattcga ccgttcttgc
     1621 ccacacgcac gagcggtatt ctcacgcatt taagtcagac tggtaccagc atccctcatg
     1681 cactgaagaa caggccgaat ggcttattca gtgttaccgc aggcgcggat gcgaggttaa
     1741 aaaagccctt agcctcgact accgtcacta gataatctcc gtcaggctcc cttactccga
     1801 acagccaccg cgtccgtccc gcacattcca gcaacggatc tggaggtaa
//
LOCUS       DAZSNM010000385         1846 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZSNM010000385 DAZSNM010000000
VERSION     DAZSNM010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.390"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..362)
                     /locus_tag="ACHW42_004310"
     CDS             complement(<1..362)
                     /locus_tag="ACHW42_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8945849.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHW42_004311"
     CDS             complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHW42_004311"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFR8945850.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          556 a          477 c          377 g          436 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttgaata atcacataac aaactc
//
LOCUS       DAZSNM010000386         1843 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZSNM010000386 DAZSNM010000000
VERSION     DAZSNM010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.391"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..206
                     /locus_tag="ACHW42_004312"
     CDS             <1..206
                     /locus_tag="ACHW42_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945851.1"
                     /translation="FGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIA
                     IYLLAGCLISAMTALLMKDNQRS"
     gene            308..1762
                     /gene="amn"
                     /locus_tag="ACHW42_004313"
     CDS             308..1762
                     /gene="amn"
                     /locus_tag="ACHW42_004313"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR8945852.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          451 a          461 c          435 g          496 t
ORIGIN      
        1 tgtttggtgc cagttatcgc tacagtggtg ctggagtcgg ttatcaggtt gccagtgtgg
       61 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      121 ggcatagcat cgccatttat ttgctggctg gatgcctgat ttctgcaatg accgctttat
      181 tgatgaaaga caatcaacgc tcgtgatagc ctggcgaaga tcatccgatc ttcgccttat
      241 acttttgttt cacattcctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      301 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      361 cgcgctgtat gagcaatctg tagttgcttt acgcaacgcc attggcaaat atattacaat
      421 tggcgaatta cctgatgaaa acgcccgtaa acaaggtctt tttgtctatc catcactgac
      481 cgtaacctgg gacggtagca caacaaatcc ccccaaaacg cgcgcatttg gtcgttttac
      541 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatctgaa
      601 tgagcaacta acgttgcttt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      661 acatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      721 gagcgctgat ttaactcgct acttcccgac aacagaactg gcgcaaatcg gcgatgaaac
      781 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      841 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagtag aacattttca
      901 accgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
      961 cagccagatc ctcgatcctg atagtcccta cattgccctt tcttgtgctg gcgggaactg
     1021 gatcactgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1081 ccagatgcca gcatggcatt taattaccgc tgatggtcag ggtattactc tggtgaatat
     1141 tggcgtggga ccgtccaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1201 tatctggttg atgattggtc actgtggcgg attacgtgaa agccaggcca ttggagatta
     1261 tgtccttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1321 tatacctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcta ccaagttagt
     1381 cagcggcagg cctggtgagg aagtcaaaca gcggctgcgt acaggtactg tggtaaccac
     1441 agatgacagg aactgggagt tacgttactc agcttctgca cttcgtttta acttaagccg
     1501 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1561 gccatacggg acactactgt gtgtttcaga taaaccgtta catggtgaga ttaagcttcc
     1621 cggccaggct aaccgttttt atgaaggcgc tatttccgaa cacctgcaaa ttggcattcg
     1681 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcacgtaaat tacgaacctt
     1741 taatgagccg ccgttccgat aataagttat gaaccttctt atcagaaata gatgtgaagg
     1801 attaagtaag acatctggca aatagcctgc aataaacgcg ggc
//
LOCUS       DAZSNM010000387         1842 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZSNM010000387 DAZSNM010000000
VERSION     DAZSNM010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.392"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1134)
                     /gene="pdeF"
                     /locus_tag="ACHW42_004314"
     CDS             complement(<1..1134)
                     /gene="pdeF"
                     /locus_tag="ACHW42_004314"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR8945853.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
     gene            complement(1316..1468)
                     /locus_tag="ACHW42_004315"
     CDS             complement(1316..1468)
                     /locus_tag="ACHW42_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945854.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            1486..1677
                     /locus_tag="ACHW42_004316"
     CDS             1486..1677
                     /locus_tag="ACHW42_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR8945855.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          581 a          412 c          374 g          475 t
ORIGIN      
        1 ttgaatccgc agcatgatgc catagttctt aaccagcatt tccatgccag ggatgcgtaa
       61 ataacaaagc gcagaccagg gggcatcacg caacgcgcga ttcagggcgc gaacatttgg
      121 cagatgaacc accgggtcca catacgcaag ccgctgaatg cgtctgacta ccgctcgctg
      181 acgagttgcc agtacagcca tataattgac aataaaagag aataccagat aactggagga
      241 ggttatggtc agctgcgtgg tatagccagg ataaatggga atgtaatttt gatagctgtg
      301 gatgctgatc atcaacacga ccgcccagag cagcgaaatc agcttataac catagcgcat
      361 tgctccccac atcatcaggg gcagcaataa tgacaaggta taattggtgc taaaaattgt
      421 gcttttttca tttaacggca tgcataacag caacaataaa gcacctaatg ccagtagcca
      481 gagcgcgaac tcttttttgg tgactttggc atcaacctgc tgttttaatt gcgaataata
      541 gctacgtaaa taaaatggat ttcgcactac ccgaatgatg aagtagcaca gcgggacacc
      601 gatcagatta cccaccagta aggcctgata attgattaaa gtcccgaggt taaaaggcat
      661 gacaccgacc agattttctc tgctcgccag taatcctaca aatgcagcaa actggaaaag
      721 tatcagaaac agcgttgcag gaaacactat ctgccagaat atacgctggg aaattaaacg
      781 ggtatcacca tgtgaaacgt tgttacgccg gggagtaaag accctgtaac cgccccaaca
      841 gagaatgata atgataataa aattagccgt taatgagagc gtttcgtaaa aacccagctc
      901 tgcatatttg cggacaaaaa tccctaaggt gatccctggc aatgccgccc agctgaaaaa
      961 catcatcatg ctgatcatca gtgccaaagg cagataaaag agaaaaacct caccggaaga
     1021 aatatgcgcg aaagtattaa tgtgggcgaa aatgggcaag attaaagaag gcaggaacag
     1081 cggaagcccc caccatttat cacgtatttt tatataagtt gcattcagtt tcatagatgc
     1141 tcagcagaat cccccacatc ctgaaggagg tgtattcaga caggcatccc acctgacttc
     1201 gaatgatgat tattcatcac tatagagagc attgattcta agtgtcatat gaaagttcca
     1261 attgatatat atcaaacaaa ataaccctga ttaatgaatt attacgttta tcatgttaat
     1321 tcatcattat tacatcatca ttgtaaataa ttaaattaac ttccataaca ttaaaatatg
     1381 tatccactga cgctttttta cataacgaag aattgaccat tttgtcctgt tgtgccttaa
     1441 tgtaagtacc gtccacagcg tgggacatac ttcaaggaac cttttgtgag tcaggcaact
     1501 agtatgcgaa aacgacaccg atttaacagt cgcatgaccc gtatcgtact gctcatcagc
     1561 tttatcttct tctttggccg ttttatctac tcgtccgtcg gtgcctggca gcaccatcag
     1621 agcaaaaaag aagctcagca atccacactc tccgtcgaat caccggtaca acgttagcgg
     1681 ttaccttctc cactttcaca gaacataacg gcacttcgct gtcggatgct tttgctcttt
     1741 gggattatca aagcggcaga tattctttca tcttaaattt tacgtctttg tcctgactga
     1801 tgtttatcct gtttggctgc gaaataaata taaaattaat at
//
LOCUS       DAZSNM010000388         1834 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZSNM010000388 DAZSNM010000000
VERSION     DAZSNM010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.393"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(320..874)
                     /locus_tag="ACHW42_004317"
     CDS             complement(320..874)
                     /locus_tag="ACHW42_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR8945856.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(871..1161)
                     /locus_tag="ACHW42_004318"
     CDS             complement(871..1161)
                     /locus_tag="ACHW42_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR8945857.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1161..1760)
                     /locus_tag="ACHW42_004319"
     CDS             complement(1161..1760)
                     /locus_tag="ACHW42_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR8945858.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFGAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          446 a          498 c          420 g          470 t
ORIGIN      
        1 ttatgggtga tcggggagag agtttcaaca gagatttgag tggtcataac gataactccg
       61 tacatttgga aattatcgcc accgacgacg ccaatcgaac tggtggcgaa ctgtgcaggg
      121 ttggcgtaac cgggtacgga aaccggcgag cctttcggct cccccacaca gcccgccata
      181 aatcgcgaat gtgactgtgc aaacgatatg aaaaaagacg cgggcgcgtc tcatatcgct
      241 ccgtaaacat ccgggacgcc aatcccgacg ccagattttg ctggcgcgtg aggaatatag
      301 ccccggataa cagattgagt caacaaacgg tttttagatc cccggaagag aatgcatcac
      361 gcatcggtag atagagcata tattcagcaa gatttagcca tacatctacc cgactgcagc
      421 acgtccggaa acaccactca gggtgtgcgt cattcagcaa ttcagccatt ttacgcttag
      481 tcatcccccg cccttcatac cgttgccgga ggacactaat caatccagga tgctctgcca
      541 gcacctcact tatgactcta tcaatacata acgcctctgc atcagtacaa tgcgccagcc
      601 aggtcttttg cttgccattg atcatctctc gcaaaaacgc ttccagctca ggtttctcta
      661 ttcccgcttt tttcatcctg cgcagggctt cattgacggc tgttttcgtc aattttttgg
      721 atgccagcaa ctggttaaac atatttcccg tcttaccgcc gccaatatac gaccagcgcc
      781 cccacatgcg cagttttccc tgaatccaga cactttccag cgtggtgaga cgaaggtgtt
      841 ccccgctttt gcctgtattt gttgggtaaa tcataaataa ccttcctttc tccagatttc
      901 ttgtgtgcga aaaacacctt ctgcatgcat caggcgtaat tcttctttgg tgtaatcgct
      961 ggtttttacc cgcccgtcga ttaaatcgtg gcatgagcta caggcaatcg ctgcctgcat
     1021 atcgtgtggt tttgtcgctg ttccgcacgt ccccgccagc ctgtaatgcg ccagcacaga
     1081 agtttcggga ttgtgattgc agtagccagg aattctgacg gtgcacatct gcccccgcgc
     1141 cgctttacgt aaatccacca ttacgcaaac tccagtaact gcgcggccac attttcgact
     1201 tcctccggag aggaaaattt acggaacagg atccagttcc acagcacatt cagtacagat
     1261 ttataaacct gctgaaactc ggtttcgtcc atgttcgcaa atgcgataga ttttgccctg
     1321 cgcccacgac taccatcagg ataaatatgc tcggtgtaaa atccggcctg aatggttacc
     1381 cactcgcgga aagcgccaaa cgactttagc aatgccgtat cccgggttct gcatgtcgca
     1441 actgtattaa ggtattgctc tgcggcatca ctcagggctg gagtgtgttc ccgacctact
     1501 gattcgcaca ggtaatcaac gaagcctgat accagttttc gttcgcgagg cgtgatcgcc
     1561 ccaccgaccg gagtccagta atcaaatccc agttgcagga gtttgaaaaa acgcttgtgg
     1621 aatgcgtagt tacgcacacg cttaaagtct gcgtgtatcc actcacctat tttgatttga
     1681 tgcaaaaaat cgcaactctc cggcgtcgcc gggagaagta atccggaaga ggtttgtttg
     1741 accagttgta tatgcgccat cgtagttctc cgctggcgca gtagaatggg tgttcagccc
     1801 gttatgtagt ataccagaat taatgccaat acta
//
LOCUS       DAZSNM010000389         1809 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZSNM010000389 DAZSNM010000000
VERSION     DAZSNM010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.396"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            173..1198
                     /locus_tag="ACHW42_004320"
     CDS             173..1198
                     /locus_tag="ACHW42_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8945859.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="ACHW42_004321"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="ACHW42_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAZSNM010000390         1807 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZSNM010000390 DAZSNM010000000
VERSION     DAZSNM010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.397"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..164)
                     /gene="yciZ"
                     /locus_tag="ACHW42_004322"
     CDS             complement(<1..164)
                     /gene="yciZ"
                     /locus_tag="ACHW42_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR8945860.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTP"
     gene            202..330
                     /gene="ymiD"
                     /locus_tag="ACHW42_004323"
     CDS             202..330
                     /gene="ymiD"
                     /locus_tag="ACHW42_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR8945861.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            complement(312..1807)
                     /gene="pdeR"
                     /locus_tag="ACHW42_004324"
                     /pseudo
     CDS             complement(312..1807)
                     /gene="pdeR"
                     /locus_tag="ACHW42_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
BASE COUNT          499 a          439 c          413 g          456 t
ORIGIN      
        1 cacggtgtct tcgggatgtc tggcgtagat tcagcaacct ggcgcaagag ttctgcttta
       61 agtatctcga gattatggat cgcagagtgg taatcgcctg ccacaaggat atctaacaca
      121 atatcaatac gttcggcaac gcgctgggca tcaaattcag acatcaagga catccttata
      181 gcaaaagggg gaaaatgaat aatgcaaaaa ttgccgctaa aagagaagtg tttaacagca
      241 acggctaatt atcatccagg aatacgatat ataatgacgg gatatagcgc taagtatata
      301 tattcatcta cttatgcgcg cttcagatag cgtttatacc agcgttcgaa ggcgacggcg
      361 ggcatcggtt tggcaaacaa aaatccttgc cgctcattga tcccgttctt ggttaaaaaa
      421 gcatcttcct ttgcactctc tacaccttcg gcgatcacct gaagattcaa tgcctgggcc
      481 acagcgacga tcgcccggac cagtgactgc gagacaggtt gtttgtgaat atctcgaaca
      541 aaaacctggt caagtttgat ggcatcgatc ggaaagcgcg ccagttgcga aagtgaagag
      601 tagccggtac caaaatcatc cagatgcact tgcgcaccta gttggctaaa ttgttgaata
      661 acagacagtg ccagttcatc attctcaatc agacaactct ctgtcagttc aacatctata
      721 gggcagtatt caaaattgag ttcctggaga acctgtttca gggcggtgaa aatggtttga
      781 tcggcgagct gacgtgcaga aatatttacc gccactcgca ggtttatgcc tttatcccgc
      841 cactttgcca cctggcgtac gacatcgaga atcacccaac ggcctaaagg cacaattagc
      901 cctgactctt cggcgtagga aatgaagtcc aacggtggaa tcaacccacg ttcaggtgac
      961 tgccaacgta ctagtgcttc cagactgcgc acttcgacac gctaggtgat tttcgataac
     1021 caactgatcg ttttccagtg ctttacgcaa gttggtatcc agccagagat attcaaatac
     1081 ccgctgattc atttctggag taaaaacgca aaattgtcct cgtccgcctt ccttcgctgt
     1141 gtacattgct gtgtcggcgt gacgaataat agtcgcgctg tccgaaccat gttcgggaga
     1201 gagcgaaata cctactgaac agctggtata aacttcaatt aaaccaatac gaaaggggag
     1261 gcgtaagcgg gtcaaaattc gtgatgccat tgcttccagc gcgctttgtg aggtattgga
     1321 tgccagtacc agaaactcat cgccacctgg acgcgccaac acctggtcat gttcgagaca
     1381 gcttaaaata gccaatgaca cgtcgcgtaa taactggtca ccaaacaaat gcccataggc
     1441 gtcgttgacc tttttaaaat tatccaaatc aagatacaca accccaactt tattgttatc
     1501 tgcctgatta atagcgtgat cgattaactc ctgcattgcg ttacgattcg gcagtccggt
     1561 gatactgtcg gtatttgcca gaatacgcag tcgctcctga gtgcggcgct cttcggtaat
     1621 gtcggtaccg gaacagatta aaaaaatctc gtttttgcca ctgccgctgt ggacaaattt
     1681 attgcgaaac agaaacagcc gctggccttt acgtgttggt atccacagtt cgacttcata
     1741 tgcattgccg cttcgaaaaa atacacggtt attgcgcctg gatgccgcag cttcacgacg
     1801 gctcata
//
LOCUS       DAZSNM010000391         1790 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZSNM010000391 DAZSNM010000000
VERSION     DAZSNM010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.398"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            69..1169
                     /gene="ompC"
                     /locus_tag="ACHW42_004325"
     CDS             69..1169
                     /gene="ompC"
                     /locus_tag="ACHW42_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR8945862.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1333..>1790
                     /locus_tag="ACHW42_004326"
     CDS             1333..>1790
                     /locus_tag="ACHW42_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8945863.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNL"
BASE COUNT          553 a          360 c          363 g          514 t
ORIGIN      
        1 ttataaatga cgccgtttaa aattcgttta gaaaatacgt ttaacgataa ttaaaaggat
       61 tctgttaaat gaaattaaaa atagttgcgg tggttgtaac tggtttgtta gctgcgaacg
      121 tagcacacgc tgccgaagtc tataacaagg atggtaataa actcgacctt tatggcaagg
      181 ttaccgctct acgttatttt actgatgata agcgtgacga tggtgataaa acttatgccc
      241 gtctcggctt taaaggagaa acgcaaatca atgatcaaat gattggtttt ggtcactggg
      301 aatatgattt taaaggctat aacgatgaag ccaacggctc gcgcggcaac aagacccgtc
      361 tggcctatgc tggtttaaaa attagtgaat ttggctctct ggactatggt cgtaactacg
      421 gtgtcggcta cgacattggt tcatggaccg atatgttgcc agaatttggt ggcgatacct
      481 ggagtcagaa agatgtcttc atgacatacc gtaccaccgg tctagcaacc tatcgcaact
      541 acgatttctt tggcttaatt gaagggctga actttgccgc gcaatatcaa ggcaaaaata
      601 aacgtactga caacagtcat ctttatggtg ctgacgacac gcgtgccaac ggtgacggtt
      661 tcggtatctc ctcaacttat gtttatgatg gctttggtat cggtgcggta tataccaaat
      721 ccgatcggac aaatgcgcag gaaagagccg cagctaatcc tctcaatgcc tccggtaaga
      781 atgcagaact atgggccaca ggtataaagt atgatgccaa caacatctac tttgcagcta
      841 attacgatga aacattaaac atgaccacct atggggatgg ttatatctct aacaaagcac
      901 aaagttttga agtggtggcg caatatcaat tcgacttcgg cttgcgccca tcactcgctt
      961 acctgaaatc gaaaggcaga gatctgggcc gctacgccga ccaggacatg attgagtata
     1021 tcgacgttgg tgcgacgtat ttcttcaaca aaaatatgtc gacctatgtt gattataaaa
     1081 tcaacctgat tgatgaaagc gactttaccc gtgccgtaga tattcgcacc gataacatcg
     1141 tcgctacggg cattacctat cagttctaac gtcctctcct gaccagccct tctctgggct
     1201 ggtttttgat ttaagtgctg ctgtatgact ccttaagtaa aaatgagcaa ttcttgatat
     1261 ttctggtcat tcacctcaat taatattatt ccatatcctc cggggacatc ttactataat
     1321 atttattgat gagtgattat gaccaatatt aatacagctt gtgtaaaaaa taatgccagt
     1381 tatcaactta ataacgcatt acctaacaaa gaaaccatct ccagcaattt ttgtgaacga
     1441 ctagaacaat ggggtaataa atcggttaat aatggtgaag aaagagcaat tgccgtggag
     1501 cgaattaaag aagcttacaa ttcgaacatg gcatctcttg atttgtcata tcttgatttg
     1561 agtgaattac cgcctatccc ctcgacagtt aatacgttaa atctggaaaa caattgtcta
     1621 acttgtcttg acttagctga caatgccagt ctcgttaata tcaacctcag cgttaataaa
     1681 attaacacga taacctttcc aaaagaacca aaactggaaa acatttatat taatcataac
     1741 aatctggaaa gtttagattt aaaaaatcag cattcattgg ttaatctgga
//
LOCUS       DAZSNM010000392         1759 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZSNM010000392 DAZSNM010000000
VERSION     DAZSNM010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.399"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..263)
                     /locus_tag="ACHW42_004327"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="ACHW42_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="ACHW42_004328"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="ACHW42_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFR8945864.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1163..1348)
                     /locus_tag="ACHW42_004329"
     CDS             complement(1163..1348)
                     /locus_tag="ACHW42_004329"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8945865.1"
                     /translation="MLPLSVNLMSSGASLAARLQQHWLWYSYNTKTGGVLAYTFGPRN
                     DETCRELLVLFTPFCIY"
     gene            complement(1373..1645)
                     /locus_tag="ACHW42_004330"
     CDS             complement(1373..1645)
                     /locus_tag="ACHW42_004330"
                     /inference="COORDINATES: protein motif:HMM:NF024169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HFR8945866.1"
                     /translation="MATVTVHCPRCHSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKPGVKEQIVEMAHNGAGGRDTARTLKIGINTVIRTLKSSRPGG"
BASE COUNT          502 a          433 c          319 g          505 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttacttttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagta ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcgt gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg actaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacga ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctcc ttaacgccgg
     1501 gttttctggc ttcataagag taagtgagct ggaaaacacg tttacatgag cgacaacgga
     1561 aacgctcatg ccgggaacaa ctacggccat gtcgataaac ttcatcggaa tgacagcgcg
     1621 gacaatgaac agtaacggta gccatggaaa accttaaata ccagtagatt accagaaact
     1681 tctgtcgatt gaagacatca cccgaagttt gaattcattc gaatactttt tacgaggttc
     1741 ttttcgccag tcctgtaat
//
LOCUS       DAZSNM010000393         1757 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZSNM010000393 DAZSNM010000000
VERSION     DAZSNM010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.400"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            311..505
                     /locus_tag="ACHW42_004331"
     CDS             311..505
                     /locus_tag="ACHW42_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945867.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            866..994
                     /locus_tag="ACHW42_004332"
     CDS             866..994
                     /locus_tag="ACHW42_004332"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945868.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(1304..1640)
                     /locus_tag="ACHW42_004333"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="ACHW42_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          527 a          391 c          375 g          464 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa atcaaca
//
LOCUS       DAZSNM010000394         1746 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZSNM010000394 DAZSNM010000000
VERSION     DAZSNM010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.401"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            453..1301
                     /locus_tag="ACHW42_004334"
     CDS             453..1301
                     /locus_tag="ACHW42_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR8945869.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1318..1599
                     /locus_tag="ACHW42_004335"
                     /pseudo
     CDS             1318..1599
                     /locus_tag="ACHW42_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
     gene            1635..>1746
                     /locus_tag="ACHW42_004336"
     CDS             1635..>1746
                     /locus_tag="ACHW42_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945870.1"
                     /translation="MAGIRSTLIKEKNMNTNYEATVATTDNIVHEVYLEGK"
BASE COUNT          498 a          398 c          411 g          439 t
ORIGIN      
        1 gcattatata gttctactct ggcgagatac gttggcatat tcatttcctt actggttgtg
       61 tgagaacttc agtaaagata ccaccaaagc ccggaagtgg tgaaataaaa ccgggcacaa
      121 cacgaaggcg catttccggt atccataaag agtcggtctt gtctgtgaaa tttaaatggt
      181 gggagtgcgc ttccggttgt aaataacgac attgctgtgt gaagtaccag ttggcggcat
      241 cggtttaatt gctggctgat gtccgccctt tttaaagtga attttgtgat gcggtgaatg
      301 cggctatgcg cacgcggcac agttaaaagt catgttagtc cttattggtt tgggtgggaa
      361 agccaactgt aattgttaac tggttaacgt cacctggagg caccaggcac cgcatcaaca
      421 aagttcactt cggtgatgaa aggaaagaga aaatgttgaa tgtagctatt gaaaaccaga
      481 acgggtggaa ttatagtgca cctgcacctc ataaaacggg tgccgggatt gctaccccga
      541 cgatgactac tgcgcataac cgcgctcagg cggttttttt atgcgtcaag cacagccaca
      601 ttcagattat ggtggggcgt gcagggcagc cgcaaggctg gccggtttcg gtagtcaccg
      661 gttgtagcaa ccctgtgcgt ctcaccaccc atgagattgc tacctctggt ggtgagtcat
      721 ttaaactgac tatcgaggct gccatcatgg ctactatcct taccctttct caccctgacg
      781 caaccatcga aaatggtcgc gctgtcacta cgtctgttgc ggttgcggag ttcttccgga
      841 aaatgcacaa aaacgttatt cagaagatcg aaactctcga atgctcgccg gagtttaacc
      901 ggcttaattt taagcccgtc acctataccg acgccaaagg cgaaaaacgc ccaatgtacc
      961 aaatcaccaa aaacggcttc gttttcctgg tgatgggctt cactggcaaa aaagccgccg
     1021 cattcaagga agcctacatt gctgagttcg accgcatgga ggccgaactg cgccagaata
     1081 acaccccacc tgcagacaag atgattccgg gtgatggtcg cactctggtt gttcacttcg
     1141 acaaattcgg caatgtcgaa ttcaccgaaa ccgttcctga tggcgcactg gtctgtaccc
     1201 tggagacttt ccggttttat ctggaaaaac agggatggac tcttgttaac cggggcgcaa
     1261 ttaaaaatat gactgtggaa caattacttt ctttaaaata aaaatggagc tacggatatg
     1321 aaccctatgc ttgattattg ttttacctct gcacctcagc agaccataac acacaccgga
     1381 atcaaaaatt tgttatggat aatcaaaaaa aacctccagt agaaaggtga tcccgattta
     1441 attccctgtg cttatggcct gtgtgacaga caagtaagcc ctgataaggc ttacactgct
     1501 gaacttattt acatgcacgg aaccatgatg aggaaaaaga aacgcaaata ttgtagtgaa
     1561 atctgcgccg aaaaagacca gatggcacac gaactttaat taactgacta ttcgaaactg
     1621 aatttatgcc agcaatggca gggattcgct caaccttaat taaggagaaa aacatgaaca
     1681 ccaattatga agccactgtt gcaactactg ataacattgt tcacgaggtt tacctggaag
     1741 gaaagc
//
LOCUS       DAZSNM010000395         1742 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZSNM010000395 DAZSNM010000000
VERSION     DAZSNM010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.402"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>1742)
                     /locus_tag="ACHW42_004337"
     CDS             complement(<1..>1742)
                     /locus_tag="ACHW42_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFR8945871.1"
                     /translation="PDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQL
                     QAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPS
                     QVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGR
                     TPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKVQVMVPVLNTANIRDGELRRL
                     STWENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPD
                     TIRVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQH
                     IDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQ
                     GWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGG
                     DSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSG
                     DAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVW
                     GDIPDNSVQPGAGNG"
BASE COUNT          308 a          603 c          462 g          369 t
ORIGIN      
        1 ccatttccgg ctcccggctg gacgctgttg tccgggatat ccccccacac gcggccaccg
       61 gtgatggcac cgggattcca cggacggcct tcaccggcga ttcttaccac cacaccactg
      121 tcaggattat cacgggcaat ccggacgcca tcctgcatat tatcagccag caggctttct
      181 ccgttacggc tgccctgcag ggccacgaac tgcgcatccc cgctgccttt tacccgacct
      241 tcaccgctga atccccgcag cccgtttccg tcatccgtgg tcagcgggtt cagccagata
      301 ccggcggact tgccgttacg gtcaaacgcc ggcagtgcca catacggttg cggatattta
      361 cgtcccggag caataaaccg tgcaggactg tctcccccgg ccagccccgc ctgacggaga
      421 acggcacggc ctgccgccac gtcccgcagc tcccgcgccg tactgaacag ccgctctgca
      481 ttcatgacct cccggtccgg tttcggctca aacacatcgt gggcagttcc tttctgtacg
      541 gcattgttga tggcatccgt ccagccctga cggttatcgg tgtacacctg cacatgctgc
      601 ttcatacgcg acagggccac gtaggctgac tcaaagccgg ccatcagttt ccggttacct
      661 tccgtgcctt caagcgcgat ggcaaaggtt tcactcgccc cctgtgcacc gtgggcggtg
      721 atggcatagg ccaggtcaat atgttgctct gcccgctcct gaccggggcg aatcacacgg
      781 gtctgctgac cgtcagaaag tgtgacactg tcaccggaaa ccgctgtcac cgtccagacg
      841 ctgttggcca cataaccgcg ctcccggtca ctcttcgtga agcgtatcct gtcaccggtc
      901 cccacccgga tggtgtccgg ggtgtacagc gtgacgcctt cagccaccgc ctcccggggc
      961 gaaatcagcc gcgtgttacc ttccgcatcc tgcagggtta tcagcccgtc atccttactg
     1021 ataccggcaa tccggtgata cacattatcc accagggcaa gggcatccgg gttattctcc
     1081 caggtggaga gacgacgcag ctccccgtcg cgtatattcg ctgtgttcag gacaggcacc
     1141 atgacctgca ccttccccag ttctccggcc ttttcccgtg catcatgaat catgctgttc
     1201 agtacgcgcc ggtcctcatt caggtgcgtg acaatcagcg tctgctcccg tgcttccggt
     1261 gtcctgccgg tatagtcgcg gacaatggct tcatacagtg tcatggggac atccggaaaa
     1321 gcctcgcctt tcagcatcgc cttctgctgc gcttctgcca gtttcgcttc ctggctgtga
     1381 ctgaactccg tcacggagtg ctccggtgcc catgcgccct ccagacgtgg cacctgagac
     1441 ggtttcacac gctcaagccc ggacagcgcc ctttccacat cccggttaat caggctgtat
     1501 accgcctccc gcagttccgg cgtctgacgc acaatctcct tcatgatgac cacatcggca
     1561 gcactgcgcg tctgctggag acggaaaggc tgaccgggcg cgatggcctg cagctggtcc
     1621 gtgtcaccgc tggccacagc ccgaccgcca ccggccgcaa tcagggcgta tgcccgtgcc
     1681 atgtcggtat tgcccaccat agagctctca tcgagaagga acagcgtgtt gctgaaatcc
     1741 gg
//
LOCUS       DAZSNM010000396         1742 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZSNM010000396 DAZSNM010000000
VERSION     DAZSNM010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.403"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>1742
                     /locus_tag="ACHW42_004338"
     CDS             <1..>1742
                     /locus_tag="ACHW42_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFR8945872.1"
                     /translation="PDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQL
                     QAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPS
                     QVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDIPMTLYEAIVRDYTGR
                     TPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVPVLNTANIRDGELRRL
                     STWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPD
                     KIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQH
                     IDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQ
                     GWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGG
                     DSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSG
                     DAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVW
                     GDIPDNSVQPGAGNG"
BASE COUNT          372 a          462 c          608 g          300 t
ORIGIN      
        1 ccggatttca gcaacacgct gtttctgctc gatgagagct caatggtggg caataccgac
       61 atggcacggg catacgccct gattgcggcc ggtggcggtc gtgctgtggc cagcggtgac
      121 acggaccagc tgcaggccat cgcgcccggt cagcctttcc gtctccagca gacgcgcagt
      181 gctgccgatg tggccatcat gaaggagatt gtgcgtcaga cgccggaact gcgggaggcg
      241 gtatacagcc tgattaaccg ggatgtggaa agggcactgt ccgggcttga gagtgtgaaa
      301 ccgtctcagg tgccacgtct ggagggcgca tgggcaccgg agcactccgt gacggagttc
      361 agtcacagcc aggaagcgaa actggcagaa gcgcagcaga aggcgatgct gaaaggcgag
      421 gctttcccgg atatccccat gacactgtat gaggccattg tccgcgacta taccggcagg
      481 acaccggaag cacgggagca gacgctgatt gtcacgcacc tgaatgagga ccggcgcgta
      541 ctgaacagca tgattcatga tgcacgggaa aaggccggtg agctgggcaa agagcaggtc
      601 atggtgcctg tcctgaacac agcgaatata cgtgacggag aactgcgtcg tctctccacc
      661 tgggagaaaa acccggatgc ccttgccctg gtggatagtg tgtatcaccg gattgccggt
      721 atcagcaagg atgacgggct gataaccctg caggatgcgg aaggtaacac gcggctgatt
      781 tcgccccggg aggcggtggc tgaaggtgtc acactgtaca ccccggacaa aatccgggtg
      841 gggaccggcg accggatgcg cttcacgaag agtgaccggg agcgcggtta tgtggccaac
      901 agcgtctgga cggtgacagc ggtttccggt gacagtgtca cgctgtcgga cggacagcag
      961 acccgggtga ttcgccccgg tcaggagcgg gcagagcaac atattgacct ggcctatgcc
     1021 atcactgccc acggtgcgca gggggcgagt gaaacctttg ccatcgcgct tgaaggcacg
     1081 gaaggtaacc ggaaacagat ggccggcttt gagtcagcct acgtggccct gtcgcgtatg
     1141 aagcagcatg tgcaggtgta caccgataac cgtcagggct ggacggatgc cattaacaat
     1201 gccgtacaga aaggaactgc ccacgatgtg ctggagccga aaccggaccg ggaggtcatg
     1261 aatgcgcagc ggctgttcag tacggcacgg gaactgcggg acgtggcggc aggccgtgcc
     1321 gttctccgtc aggcggggct ggccggggga gacagtccgg cacggtttat tgcaccgggg
     1381 cgcaaatatc cgcagccgta tgtggcactg cctgcttttg accgtaacgg caagtccgcc
     1441 ggtatctggc tgaacccgct gaccacggat gacggaaacg ggctgcgggg attcagcggt
     1501 gaaggacggg tgaaaggcag cggggatgcg cagtttgtgg ccctgcaggg cagccgtaac
     1561 ggcgagagcc tgctggctga taatatgcag gatggtgtcc ggattgcccg tgataacccc
     1621 gacagcggtg tggtggtgag aatcgccggt gaaggtcgtc cgtggaatcc cggtgccatc
     1681 accggtggtc gcgtgtgggg ggatatcccg gacaacagcg ttcagccggg agccggaaat
     1741 gg
//
LOCUS       DAZSNM010000397         1735 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZSNM010000397 DAZSNM010000000
VERSION     DAZSNM010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.404"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            71..166
                     /locus_tag="ACHW42_004339"
     CDS             71..166
                     /locus_tag="ACHW42_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFR8945873.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            292..1029
                     /gene="erm(B)"
                     /locus_tag="ACHW42_004340"
     CDS             292..1029
                     /gene="erm(B)"
                     /locus_tag="ACHW42_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFR8945874.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          642 a          288 c          278 g          527 t
ORIGIN      
        1 aattttgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
       61 gggattcgtc atgttggtat tccaaatgcg ttatcaaatg cgttatgtag ataaaacatc
      121 tactgttttg aaacagacta aaaaaagtga ttacgcagat aaataaatac gttagattaa
      181 ttcctaccag tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc
      241 gtttaacttc tgtatttgtt tatagatgta atcacttcag gagtaaatta catgaacaaa
      301 aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca aataataaaa
      361 caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa agggcattta
      421 acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga cagtcatcta
      481 ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat tcaccaagat
      541 attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa tattccttac
      601 catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc tgacatctat
      661 ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac actagggttg
      721 ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga atgctttcat
      781 cctaaaccaa gagtaaacag tgtcttaata aaacttaccc gccataccac agatgttcca
      841 gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt
      901 caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt aaacaattta
      961 agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt atttaacggg
     1021 aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta ctaaagggaa
     1081 tgtagataaa ttattaggta tactactgac agcttccaag gagctaaaga ggtccctaga
     1141 ctagcaagaa gtacacaaga agccttaaag attatagaaa agcttttcca tgaaatgctt
     1201 aacattattt aatgttaaca tgtggctttg tggtaactac cacgagtggt aacttgtaac
     1261 atgaaaaaag ttacaagtta caagtggttt atttacgaac ataaaggcta ggaaagcact
     1321 tgataagtat atcaagtgct ttttttccgt tcgattttac tatccaagat accgcttgac
     1381 gtacctaata ttttagtgtg ctaagggctt tgctttttct ggttatagtt cgttcaaaaa
     1441 agaccgcaag ggtaaatgca cgatcataga tcgcccttgc agctttttaa tctctccact
     1501 tttttataac caagcaaacc accaagcaca ctgaaaaaat aattaggaac taaagcgaag
     1561 tgcgtatctt ggataaatct tcactcgatt ttaaaagatt aagcacccag ccaaagcgcc
     1621 taaaaaaata atttttttaa agaaaggaag taatttaaat gactacaatt ttaagcgact
     1681 acaataaatc tatcctaatt gaattactaa acaccaatcg ccaaattatc gtcgt
//
LOCUS       DAZSNM010000398         1733 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZSNM010000398 DAZSNM010000000
VERSION     DAZSNM010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.405"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(9..215)
                     /locus_tag="ACHW42_004341"
                     /pseudo
     CDS             complement(9..215)
                     /locus_tag="ACHW42_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(268..519)
                     /locus_tag="ACHW42_004342"
     CDS             complement(268..519)
                     /locus_tag="ACHW42_004342"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945875.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGTFSYIFGSIVATLGMG
                     LVVDYLRWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
     gene            971..1255
                     /locus_tag="ACHW42_004343"
     CDS             971..1255
                     /locus_tag="ACHW42_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFR8945876.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            1255..1530
                     /locus_tag="ACHW42_004344"
     CDS             1255..1530
                     /locus_tag="ACHW42_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR8945877.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          476 a          434 c          407 g          416 t
ORIGIN      
        1 ctcactcctt atttacccgg gaaatacttc tccagcaatg catacaggat ggcagcggtt
       61 tcagcatcca ttatgccgtc ataatttttc tgccggaaat gaagctgaaa agcgcggaca
      121 agcgccttat atccgtcatc cgtctccaca ccagaaatac tgtatccata ggtcttcagc
      181 cgtgacacga tatcaacctt atccggaatg ccctgcgatt caaaaaataa atatttagtc
      241 agtaatttta tgtttttcct gaaaaaatta ctctttttcg aactccagtg agcgttcacg
      301 actcatcagt gtgaaaatga tggcaaaaac agcagacagt atcagtacga taaatgtggc
      361 cccccatctc agataatcga caacaagtcc cattcccagt gtcgcaacaa ttgagccgaa
      421 gatataggaa aatgtgcctg acagccctaa cagaataagt ttacgcgtaa atcgccctga
      481 atccagaaga gcattaagta tcacataaac aggttccata ccagtcctga ccgcatcagg
      541 acgccagtaa ataaaatctc gtagtccacc ggcatcagag acaagagtaa tcgtcgttac
      601 cggtatttcg actggtttgt actggtgtga gcggacaaaa ctactgtacg tctctcatac
      661 acgctcgtaa gcggccctca cggcccgcta acgcggagat acgccccgac tgcgggtaaa
      721 cccttgtcgg gaccactccg accgcgcaca gaggcatttt catcgctgac aggccgcatg
      781 gcctcgcaga gccggagggg gactggtgat attcggacga ccgtaccggc ttgcgccggg
      841 cttcggcggt tttacacctg tgctacatgt aaccaccgta tgccgcctta tatgcgctga
      901 ctcgcattat ttgccttgtt ccgtctgaaa gtaatcacta tgattaaata tgattaacaa
      961 ctaatcgggt atgcaaatga aaaacaatac cgcacaagca acaaaagtaa ttaccgcgca
     1021 tgtgccatta cctatggctg ataaagtcga ccagatggcc gccagactgg aacgctcccg
     1081 gggctgggtt atcaaacagg cgctttctgc atggcttgcc caagaggagg agcgtaatcg
     1141 cctgacgctg gaagccctgg acgatgtgac atccggacag gttatcgacc atcaggctgt
     1201 acagtcctgg gcggacagcc tcagtactga ccatctgtta ccggtgccac gctgatggaa
     1261 ctgaagtgga ccagtaaggc actttctgat ttggcgcggt tatatgattt tctggtgctg
     1321 gccagtaaac ctgctgccgc cagaacggtg cagtccctga cacaggcgcc ggtcattctg
     1381 ttaactcatc cacgtatggg agaacagttg tttcagtttg aacccaggga ggtcagacgg
     1441 atttttactg gcgagtacga aatccgttac gaacttactg gccagactat ttatgtattg
     1501 cgcctgtggc acacacgaga gaacaggtag attgggtgtg gaggcaactt taacatagcc
     1561 tgcaggtatt aattctggag cattgttatc aggccagata ttttgcattc cattaaataa
     1621 tcttgtagga gataaaataa attacgatga aataacgaaa attaccgctc gtgactggcg
     1681 gcaatatcgc gctccgggct ggcaaataac ccatcagaaa cgttattgcc aga
//
LOCUS       DAZSNM010000399         1718 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZSNM010000399 DAZSNM010000000
VERSION     DAZSNM010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.406"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1718
                     /gene="sat"
                     /locus_tag="ACHW42_004345"
                     /pseudo
     CDS             1..1718
                     /gene="sat"
                     /locus_tag="ACHW42_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          612 a          307 c          346 g          453 t
ORIGIN      
        1 actaaagaca aagatattgt gttcacaaat gggggaaatg ttctgtttaa ggataatttg
       61 gattttggtt gcggtggtat tatctttgac gaaggccatg aatataacat aaacggtcag
      121 agatttacat ttaaaggagc aggaattgat atcggaaaag aaagcattgt aaactggaat
      181 gcattgtatt ccagtgatga tgttttacac aaaataggcc ccggtactct gaatgttcaa
      241 aaaaaacagg gggcaaatat aaagataggt gaaggaaatg ttattcttaa tgaagaagga
      301 acatttaaca atatatacct tgcaagcgga aatggtaagg taatactaaa taaagataat
      361 tcccttggca atgatcaata tgcggggata ttttttacta aacgtggtgg cacgctagat
      421 ttaaatggac acaatcagac ttttactaga attgccgcca ctgacgatgg aacaacaata
      481 actaactcag atacaaagaa agaagccgtt ctggcaatca ataacgaaga ctcctacata
      541 tatcatggga acataaatgg caatataaaa ctaacgcaca atattaattc tcaggataag
      601 aaaactaatg caaaattaat tctggatggt agtgtcaaca caaaaaatga tgttgaagtc
      661 agtaatgcca gtcttaccat gtaaggccat gcaacagagc atgcaatatt cagaagcaca
      721 gcgagtcatt gctccctggt atttctttgt ggaacggact gggtcaccgt tttgaaagaa
      781 acagagagtt catataataa aaagttcaat tctgattaca aaagtaataa tcagcagacc
      841 tcatttgatc agcctgactg gaaaaccggg gtgtttaaat ttgatacatt acacctgaac
      901 aatgctgact tttcaatatc acgcaatgcc aatgttgaag gaaatatatc agcaaataaa
      961 tcagctatca caatcggcga taaaaatgct tacattgata atcttgcagg gaaaaatatt
     1021 actaataatg gttttgactt caaacaaact atcagtacta atctatccat aggagaaact
     1081 aaatttacag gtggcatcac tgcacataac agccaaatag ccataggtga tcaagctgta
     1141 gttacactta atggtgcaac ctttctgaat aatactccta taagtataga taaaggagca
     1201 aaagttatag cacaaaattc catgttcaca acaaaaggta ttgatatctc cggtgaactg
     1261 actatgatgg gaatccctga acagaatagt aaaactgtaa cgccgggtct ccactacgct
     1321 gctgatggat tcaggctgag tggtggaaat gcaaatttca ttgccagaaa tatggcatct
     1381 gtcaccggaa atatttatgc tgatgatgca gcaaccatta ctctgggaca gcctgaaact
     1441 gaaacaccga ctatatcgtc tgcttatcag gcatgggcag agactctttt gtatggcttt
     1501 gataccgcct atcgaggcgc aataacagcc cccaaagcta cagttagcat gaataatgcg
     1561 atctggcatc taaatagcca gtcatcaatt aatcgtctag aaacaaaaga cagtatggtg
     1621 cgttttactg gtgacaatgg gaagtttaca acccttacag tggacaacct tactatagat
     1681 gacagtgcat ttgtgctgcg tgcaaatctg gcccaagc
//
LOCUS       DAZSNM010000400         1673 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZSNM010000400 DAZSNM010000000
VERSION     DAZSNM010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.407"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..246
                     /locus_tag="ACHW42_004346"
     CDS             <1..246
                     /locus_tag="ACHW42_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR8945878.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACHW42_004347"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACHW42_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR8945879.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..>1673)
                     /gene="ybjG"
                     /locus_tag="ACHW42_004348"
     CDS             complement(1109..>1673)
                     /gene="ybjG"
                     /locus_tag="ACHW42_004348"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR8945880.1"
                     /translation="LINATPDSAPWMISLAIFIAKDLITVVPLLAAVLWLWGLTAQRQ
                     LVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFPSDHGTVIFTFA
                     LAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIGCLSAQIIWQAM
                     GHKLYQRLQSWYRVCFALPIRKGWVRD"
BASE COUNT          451 a          435 c          416 g          371 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata aga
//
LOCUS       DAZSNM010000401         1665 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZSNM010000401 DAZSNM010000000
VERSION     DAZSNM010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.408"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            230..1665
                     /gene="pdeC"
                     /locus_tag="ACHW42_004349"
                     /pseudo
     CDS             230..1665
                     /gene="pdeC"
                     /locus_tag="ACHW42_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
BASE COUNT          459 a          353 c          358 g          495 t
ORIGIN      
        1 tgtggttttt cagtggcatt aagggcatat cttcccgccg cctctgtatt cctgtagaaa
       61 attaattttg aatatcaatg aattattttc atccaggtga cgattaaaaa ggtatcaatt
      121 tcaaatcagg caaaagtgct atttataccg taagatttat ctaaagacgt cggtagccag
      181 ggtttcacct tgcaatggcc gggtataaac aggcaggaaa ttgatagcaa tgagtcatcg
      241 tgcacgacac caattactgg cgttgccggg cattatcttt ttagttctct ttcccatcat
      301 tctttcgcta tggattgcct tcctttgggc aaaatcagaa gtgaataatc agctccgaac
      361 ctttgctcaa ctggcactgg ataaatccga gctggtcatt cgccaggcag atttagtgag
      421 cgatgcagct gaacgctatc aggggcaagt ttgcactcca gcccatcaaa agcgaatgtt
      481 gaatattatt cgtggctatc tttatattaa tgaattcgtg ataaccattt tttatgctca
      541 tcgctgatag cgcctgtaaa cggctatacg attgcaccgg ccgattataa gcgtgaacct
      601 aacgtttcta tctattatta ccgcgatacg ccttttttct ctggctataa aatgacctat
      661 atgcagcggg gaaattatgt ggcggttatc aaccctctct tctggagtga agtgatgtct
      721 gatgacccga cattgcaatg gggtgtgtat gatacggtga tgaaaacctt tttctcgtta
      781 agcaaagagg cctcggcagc aacgttttct ccgctgattc atttgaagga tttaaccgta
      841 caaagaaatg gctatttata tgcgacaatt tattcgacaa aacgcccaat tgcagccatt
      901 gttgcgactt catatcaacg tcttataacc catttttata atcatcttat ttttgcgttg
      961 cccgccggta ttttggggag tcttgttctg ctattactct ggctacgtat tcgacaaaac
     1021 tatttatctc ccaaacgtaa attgcaacgc gccctcgaaa aacatcaact ttgtctttat
     1081 taccagccaa taatcgatat caaaacagaa aaatgtatcg gcgctgaagc gttgttacgt
     1141 tggcctggtg agcaggggca aataatgaat ccggcagagt ttattccgct ggcagaaaag
     1201 gaggggatga tagaacagat aactgattat gttattgata atgtcttccg cgatctgggc
     1261 gcgtacctgg caacacatgc cgatcgctat gtttctatta acctgtcggc ctccgatttt
     1321 catacgtcac ggttgatagc gcgaatcaat cagaaaacag agcaatacgc ggtgcgtccg
     1381 cagcaaatta aatttgaagt gactgagcat gcatttcttg atgtcgaaaa aatgacgccg
     1441 attaattctg gctttccgcc aggctggtta cgaagtggca attgatgatt ttggtattgg
     1501 ctactctaac ttgcataacc ttaaatcatt gaatgtcgat attttgaaaa tcgataaatc
     1561 gtttgttgaa acgctgacca cccacaaaac cagtcatttg attgcggaac acatcatcga
     1621 gctggcgcac agcctggggt taaaaacgat cgctgaaggc gtcga
//
LOCUS       DAZSNM010000402         1618 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZSNM010000402 DAZSNM010000000
VERSION     DAZSNM010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.409"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1618
                     /locus_tag="ACHW42_004350"
                     /pseudo
     CDS             1..1618
                     /locus_tag="ACHW42_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
BASE COUNT          394 a          406 c          484 g          334 t
ORIGIN      
        1 taacctcgtc gctgaaaggt aaacctgttt ctgcgcagct aacggtaggc gtggtcgatg
       61 aaatgatcta cgcgctgcaa ccagaaatcg cgccgaatat cggcaaattt ttctatccgc
      121 tggggcgtaa caatgtgcgt accagctcca gtctgtcgtt tatcagctat gaccaggcgc
      181 tctccagcga gccggttgcg cctggcgcga ctaaccgcag cgagcggcga gtaaaaatgc
      241 ttgaacgtcc acggcgtgaa gaggtggata ccgcggcatg gatgccgtca ctcacaaccg
      301 ataaacaagg caaagcgtat ttcacgttcc tgatgcctga ttcgttaacc cgctggcgta
      361 tcaccgcgcg tgggatgaac ggcgacgggc tggtcgggca ggggcgtgct tatctgcgtt
      421 cggaaaaaaa tctctacatg aagtggagta tgccaacggt gtatcgcgtg ggcgacaaac
      481 cggcggcagg actgtttatc ttcagtcagc aggataacga accggtggcg ctggtgacta
      541 aatttgcagg cgctgagatg cgccagacgc tgacgctgca caaaggggcg aattatattt
      601 cgctgacgca gaatattcag caatctggct tgttaagtgc agaactgcaa caaaatgggc
      661 aagtgcagga cagcattagc acaaaactgt cttttgtgga caacagctgg cccgttgaac
      721 agcagaaaaa tgtcatgctc ggcggtggcg agaacgcgct gatgttgccc gagcaggcga
      781 gtaatatccg gctacaaagt agtgaaacgc cgcaggagat tttccgcaac aatcttgatg
      841 cgttagtcga tgaaccgtgg ggtggggtga tcaacaccgg tagccgtctg atcccgctca
      901 gtctcgcctg gcgttcgctt gccgatcatc aaagtgccgc cgctaacgac attcgtcaga
      961 tgattcagga taaccgtctg cggctgatgc aactggcggg gcccggagcg cgctttacct
     1021 ggtggggtga agatggcaat ggtgacgcct tccttacggc atgggcatgg tacgccgact
     1081 ggcaggccag ccaggcgctc ggcgtaacgc aacaaccgga atactggcag catatgctcg
     1141 acagttatgc cgagcaggcg gataatatgc cgttattgca tcgggcgctg gtgctggcgt
     1201 gggcacagga gatgaatctg ccgtgcaaaa cgttgttgaa agggggggat gaagctatcg
     1261 cccggcgcgg aactaaagat gaagatttct ctgaggaaga catccgcgat atcaatgaca
     1321 gcctgatcct cgatacaccg gaatctccac tggcagatgc ggtggcaaac gtcttaacca
     1381 tgacgttgct gaaaaaagcg cagttgaagt ccacggtgat gccacaggtt cagcaatatg
     1441 cgtgggataa agcggcaaac agcaatcagc cgctggcgca cacggttgtg ctgctcaata
     1501 gcggtggtga cgctacccag acggccgcta ttttaatggt ttgaccgctg agcaatccac
     1561 tattgagcgc gcgctggcca tgaactggct ggcgaaatat atggcgacaa tgcctcca
//
LOCUS       DAZSNM010000403         1606 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZSNM010000403 DAZSNM010000000
VERSION     DAZSNM010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.410"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..389)
                     /locus_tag="ACHW42_004351"
                     /pseudo
     CDS             complement(1..389)
                     /locus_tag="ACHW42_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            818..1606
                     /gene="virF"
                     /locus_tag="ACHW42_004352"
     CDS             818..1606
                     /gene="virF"
                     /locus_tag="ACHW42_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HFR8945881.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          504 a          299 c          296 g          507 t
ORIGIN      
        1 acccgctgac gtcgatattc ccttggtgag agataaccca acgcactatg cggatggttt
       61 tcattgtaat gctcgatcgc cactgcaagg ttatgcaatg ccgttcttgg tttcggcatg
      121 aacgcgatgc agtcttcctt catcgttttc atgaaccgtt ctgctattcc attactctga
      181 gggctactga ctgccgtcgt gcagggctcc agattcaact ctctggcgaa ctgccgtgtt
      241 tcatgagccg tataagccga accgttatcg gccactggat tggccgttcc ggtcctttat
      301 cgccatatcg ctcctgacat gacatcctgc accgttgctt tgtcataatc tcccgtgcct
      361 gctgcccagt ctatggcttc acgatcgcaa tatggaacgt aacgctgtca tcgggagaat
      421 cgtcaatgcg gcattcggtt aacgcttacc aatatcaaca ccaaccggat ttgtggagcc
      481 tccagtctga aggggcttta tgcgttccgt ataggatatt atgatgctgg agttttgcaa
      541 agctccactc ctttccgaac atttacacag atattgccta agaaaagtga aaccataata
      601 tggttatagt ccctttcagt gcaaatactt agcttgttgc acagagaaat agaagctgca
      661 taagctcttt cttcaaaaaa tgtaaataaa gttaaatata ggaaaaatta cttaatctat
      721 cttaataacg gaaagatttt gtatacaatc actgttacag aaatttctta gttactctgt
      781 aaacactaaa tatagtttgg tatattctgt tgaatttatg atggatatgg gacataaaaa
      841 caaaatagat ataaaggttc gcttgcataa ctatattatt ttatatgcaa aaaggtgttc
      901 aatgacggtt agctcaggca atgaaacttt gactatcgat gaagggcaaa ttgcttttat
      961 agagcgaaat atacaaataa acgtctccat aaaaaaatct gatagcatta atccatttga
     1021 gattataagc cttgacagaa atttattatt aagcattatt agaataatgg aaccaattta
     1081 ttcatttcaa cactcctatt ctgaggagaa aagggggtta aacaaaaaaa tattcctcct
     1141 ctctgaggag gaggtttcta tcgatttgtt caaatctata aaagagatgc ctttcggcaa
     1201 aagaaagatc tatagtttag cttgcctttt atcagctgtt tctgatgagg aagctttata
     1261 tacttcgata tcgatagctt cttctcttag tttttctgat cagataagga agattgttga
     1321 aaaaaacatc gagaagagat ggcgtctttc tgatatttca aataacttga atttatcaga
     1381 aatagctgtt agaaaacgat tggagagtga aaaattaaca tttcaacaaa tccttcttga
     1441 tattcgcatg catcatgcag caaagctttt attgaatagt caaagctata ttaatgatgt
     1501 atcaagactt atcggaatat caagcccatc ttattttata aggaaattta atgaatatta
     1561 tggtataact ccaaagaaat tttacttata tcataaaaaa ttttaa
//
LOCUS       DAZSNM010000404         1601 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZSNM010000404 DAZSNM010000000
VERSION     DAZSNM010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.411"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(333..479)
                     /locus_tag="ACHW42_004353"
                     /pseudo
     CDS             complement(333..479)
                     /locus_tag="ACHW42_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            533..715
                     /locus_tag="ACHW42_004354"
     CDS             533..715
                     /locus_tag="ACHW42_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR8945882.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(816..1145)
                     /locus_tag="ACHW42_004355"
     CDS             complement(816..1145)
                     /locus_tag="ACHW42_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR8945883.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1317..>1601)
                     /locus_tag="ACHW42_004356"
     CDS             complement(1317..>1601)
                     /locus_tag="ACHW42_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945884.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          436 a          368 c          376 g          421 t
ORIGIN      
        1 agcaatgtct gtcgcacaac aaccacaatg tcacaaataa aaaatgagaa taaccagttc
       61 cctgaaggcg acgtagcccg gtacaagagt aacccacgtt agcgcttggc gacggcggaa
      121 caaagctgat tcgcgacgta agaaagtggc aggctcatat gacggacctc tgtaatgcgg
      181 tatccaaccc gcgaatatca gcatgattca ccgtcggatt tactgctacg tcgccctcag
      241 ggaattaaac ctaaaaagaa aatcgtcaaa attagagatt ccggtttgac acggaaagtc
      301 atatcagcgt caacggagca ccgtattgac gcttatttat tggtgagaac tacgttccat
      361 ggcaggagtt cgtcaacacg gttggagggc cattcaggca gtatgctcag aatatggcgc
      421 agatacgctt ccggatcgat accgttcaga cggcaggtgc ctgcttttcc ggcaacaagc
      481 aggaatctca gcgctgaagc aggaattaga ggtaaaaacg ccctaccggg ccatgaatca
      541 cccggtcatt ggagtagtaa ccaaagcaga tttagccagc atggaacaaa tatctttggt
      601 gaaaagttgg ttacgggaag ctggagcgca caacgtgtta gtaaccagtg cagttaataa
      661 caatggagtt acagaacttt ttgccttgct gcatacagaa gaaggctgtt gttaatttcg
      721 attggttctc agatgagaat gatgacggtg atacacagat aagaatcaat gagcaatgag
      781 ggttgccggg caaccctcat tgaataaaac gggaattact tcgcttcgcc gtttttcatt
      841 tcgcccatag ctttcagctt tttggagata tcgcggcgtt ctttggagag ctcggcattt
      901 ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt tcatgctggc gatgatgccc
      961 tggattgctt caacgctcat ccctggcttg atgtaatcgc tcaggttgtc cagcaacagg
     1021 acgcgcttct ggttgtcacg gatctttttc tcaacgtcct gaatttcacg ttgcagtttg
     1081 ttcttacgac ggaacagacg aacaaattcc agtacgtcct ggaatgaagg cttggtagtt
     1141 tccattttta tacccctgat aatgtgagag tcggattcgt ttaagcaacc gctattcgtt
     1201 agggccaaca ttactcatag ctaccgcaga tgacaatgct tttatccctt ttcactatca
     1261 taccctttat ccttgctgaa tcgaagcagc agcaagatga ttctgaagtt caggaattat
     1321 ttgcgcaagg cccggcaaat cagattcgat agcaactcaa gctgatgtgc cgtttccatg
     1381 ttcagccaca cataaccata gattggtgtt tccacgactt tcaggtcatg gtggttatcg
     1441 agcaactgac gcagctcttc cacagcatcg ttcaattttt ccgtattggc aaaaaccggc
     1501 tgcggattac cttcgtacag cgcatgaaca aggctcagca gtatttgctg catcatgtgc
     1561 tgggtatcac aaagtttttg cgcgttcaat aacacgaaat g
//
LOCUS       DAZSNM010000405         1580 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZSNM010000405 DAZSNM010000000
VERSION     DAZSNM010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.412"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHW42_004357"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHW42_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFR8945885.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1580)
                     /locus_tag="ACHW42_004358"
     CDS             complement(1269..>1580)
                     /locus_tag="ACHW42_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HFR8945886.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          481 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac
//
LOCUS       DAZSNM010000406         1561 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZSNM010000406 DAZSNM010000000
VERSION     DAZSNM010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.413"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(174..1367)
                     /gene="tyrB"
                     /locus_tag="ACHW42_004359"
     CDS             complement(174..1367)
                     /gene="tyrB"
                     /locus_tag="ACHW42_004359"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR8945887.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT          423 a          400 c          375 g          363 t
ORIGIN      
        1 agggaaagga attccagata attgggataa acttaacaat aaaagaactg acaacgtttt
       61 attatctctc acgtagaaca acggcatcaa aaaaatcatc tacaaagaaa tagtgaaagc
      121 gacgacagtt taatttcact gcaggctggg taactccagc ctgctttcct gcattacatc
      181 accgcagcaa acgcctttgc cacacgttgt acatttgccg tatttaaccc ggcgacacac
      241 atgcgaccgc tggcgatgag atagacacca aattcttcac gtagtcggtc aacctgagcg
      301 gcacttaaac cggtataact gaacatgccg cgctgattaa gcagataatc gaaattgcgt
      361 tctggcatct ctgtgcttaa taccttcacc aattcctgac gcattgccag aatgcgagta
      421 cgcatctctt ctacttccgc cagccagctg gctttcaatg cctcgtcatt cagcactgca
      481 gccaccacct gcgcaccaaa attcggcggg ctggagtagt tgcggcgaac tgttgctttc
      541 aattgcccca gtacgcggcc agcggcttcg gcatcttcac acagaacaga aagtccgccg
      601 acgcgctcgc cgtaaaggga gaaaattttc gagaacgaat tgctcaccag agcgggtaat
      661 ccagcactgg caatggcgcg aatagcgtag gcatcctctt ccataccggc actaaatcct
      721 tgataggcaa tatcgaggaa tgggataagc tcgcgggctt tgagaatttc aatcaccgca
      781 tcccactgat cattagtgag atcggcaccc gttgggttgt ggcaacatgg atgcagcaac
      841 acaatactgc gggcaggtaa tgttttcagc gtcgccaaca ggtcattaaa gcgcacgctg
      901 ttagtcactt cgtcatacca ggggtaagta ctcacttcga atccagcccc ggcgaatatt
      961 gctacgtggt tttcccaagt aggatcgctg acccagacgc ctgattccgg gaagtagcgt
     1021 ttcaggaaat ccgcgcccac tttcaatgcc cctgagccgc caagggtttg aatggttgct
     1081 acgcgctgtt gttgcagtac cggatggtcc gcaccaaaca gcagcggcgc aatggcatgg
     1141 cgatagctgt taagcccttc catcggtaaa taaagcgaag cgccatgggg ctgcgcattc
     1201 aggcgcgctt ccgcctccgc cacggctttc aattgtggaa taattccgtc ttcgttgtag
     1261 tacagaccga tacttaaatt cactttgtcg ctgcgagggt cttctttaaa acgctccata
     1321 agcgtaagaa tcgggtcgcc agcgtaggcg tcaacttttt gaaacacgcg atggttctcc
     1381 aggtttacgg gcaggtggtt aaaacacaat aaaccggaag aaggcgaaga tcgagtggat
     1441 gttcaggagc gaacggcaat tagcaacaga gtgagactaa tgataaacgt acatccgcca
     1501 gaggcacgac ctttataaga gcatgaaaga acatcaactt attgaatttt taggatttta
     1561 t
//
LOCUS       DAZSNM010000407         1554 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZSNM010000407 DAZSNM010000000
VERSION     DAZSNM010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.414"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            624..1223
                     /locus_tag="ACHW42_004360"
     CDS             624..1223
                     /locus_tag="ACHW42_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFR8945888.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
BASE COUNT          408 a          372 c          353 g          421 t
ORIGIN      
        1 tggcttcacc cgtgttaata tcctggataa ctgtgtttga aggatttctg ttaataatct
       61 gctcctgcaa ctcacctgat gagtattagt ggtaatacat aatcttactc gtaaaagagt
      121 ataccttatg tcattaaaca gtcttccggc gtgttgttca atcctgtacc tctgttactt
      181 atctggtacg ttgatatgtg taatgctacg ttaattgcaa tatctggtta tggctttata
      241 ccttcagcat ttaaaatatt ttattaccgg agaaacaggg aagtggaaag actttcggga
      301 taatgtcagg ccattctgac gttaatcaca gtaccaaata acgagagggt aatggaagtt
      361 aaaacctgcg taagttcctc tgttgttcac tggaaaaagt gattgcccac acgcgttata
      421 aaggcctttc cgctgcagcc tggttaacag agcttcaggc gttatatgcg ctgtatggct
      481 gttacgtatg ctgttctttg cctggttctg atacccgtgg aagtcgttac cggaggaaac
      541 acgttcaccc tactacctga gaacatcttc ttcaaaaatt acggcaacgc ttcttcctgt
      601 gttacagaac acgaggaaga aacatgcaaa cttataacac caatcccgct tacgaaatgg
      661 ctccccagct cctggagcac tttaaccagc atctggattc attattcggg gtctattcga
      721 aactgttacc gttcagaatg gattttgctt accgaaaaaa caccctcagc tacagatgtg
      781 cctgcaggta cgccatgtgc gcggagatgc tgcaactgat taacgaggtg ggagagaaac
      841 tggtcgggta tgcctgggtg atggaatata cagaacgtaa agggcttcac attcattttg
      901 tgggctatct gaacggccag agccacagga gttcgtatct ggtatccaga ttgatgggag
      961 atatctggag gcgggttacc gagggtaatg gttactacca ctggtgccgg tttaataaaa
     1021 attatccggt aaacattaac cacgtcattc actattcaga tcacaaagcg gtgaatgatt
     1081 tacgctatgc catcagctac ctggccaaac gggaacagaa agaatgtggc atcatcctga
     1141 aatgcagcgg gcttcctgaa aaaagtaacc ggggtcgacc acgactggac agccccctgc
     1201 cggggatatg tgctctggtg taaacccctg aacgacaagt acgggctggc gctaatcttt
     1261 gctgtcggtt ccggcggcca cttcagcggc actgcgcatt ttttacggcg tggtcattcc
     1321 gtatgaacca tacggagatt taaccatgac acattcttta ccattccttc cggcaaccga
     1381 tacagcacat cccccaatct ccatctctgc ttactgactg ttttttacgt cagtgggcca
     1441 ttgtgtgaca accacagtgc tgtaacagca accagcctgc tttacccaaa ctccagtcca
     1501 gattttaact gaaccgccat tcacaccctg accacgctgc ccgtaccggg acgg
//
LOCUS       DAZSNM010000408         1541 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZSNM010000408 DAZSNM010000000
VERSION     DAZSNM010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.415"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            147..503
                     /locus_tag="ACHW42_004361"
     CDS             147..503
                     /locus_tag="ACHW42_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR8945889.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            507..>1541
                     /gene="dapE"
                     /locus_tag="ACHW42_004362"
     CDS             507..>1541
                     /gene="dapE"
                     /locus_tag="ACHW42_004362"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR8945890.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNA"
BASE COUNT          373 a          352 c          407 g          409 t
ORIGIN      
        1 tgtttacgtt aaaaatgttt aatattattt aatagttgtt aatttgaata cttcgataat
       61 gttatatttt ctgataatca tttgcaggca aaatgttttc acccttaaat gagtatttat
      121 tctcataaat cgaaaaagga ttcattatgg ttacacttta cggcatcaaa aattgtgaca
      181 ccattaaaaa ggctcgccgt tggctggaag ccaataacat cgactatcgt tttcatgatt
      241 accgcgtcga tgggctggac agcgaattat tgaacggttt tatcaacgaa ttaggctggg
      301 aagcgttact caacacccgt ggtacaacct ggcgtaaact ggacgaaact acccgcaata
      361 aaatcaccga tgcggcctct gcggcggcat taatgactga aatgcctgca attatcaaac
      421 gtccattgct ctgcgcgcct ggtaagccta tgctgctggg tttcagtgat tccagttatc
      481 agcaattttt ccatgaggtg tagtctatgt cgtgcccggt tattgagctg acacaacagc
      541 ttattcgccg cccttccctg agtcctgatg acgcaggatg tcaggctttg ttgattgaac
      601 gtttgcaggc gatcggtttt accgttgaac gcatggattt tgccgatacg cagaattttt
      661 gggcatggcg tgggcaaggt gaaacgttag cctttgccgg gcataccgac gtggtgccgc
      721 ctggcgacgc cgatcgttgg atcaatccgc cgtttgaacc caccattcgt gacggcatgt
      781 tattcgggcg cggtgcggca gatatgaaag gctcgctggc ggcgatggtg gtagcagcag
      841 aacgttttgt cgcacaacat cccaaccata cggggcgact ggcatttctg atcacttctg
      901 atgaagaagc cagtgcccac aacggtacgg taaaagtcgt cgaggcgtta atggcacgta
      961 atgagcgtct cgattactgc ctggtcggcg aaccgtcgag tatcgaagtg gtaggtgatg
     1021 tggtgaaaaa tggtcgtcgc ggatcgttaa cctgcaacct caccattcat ggcgttcagg
     1081 ggcatgttgc ctacccacat ctggctgaca atccggtaca tcgcgcagca cctttcctta
     1141 atgaattagt ggctattgag tgggatcagg gcaatgaatt cttcccggcg accagtatgc
     1201 agattgccaa tattcaggcg ggaacgggca gtaacaatgt tattccgggt gaactgtttg
     1261 tgcagtttaa cttccgcttc agcaccgaac tgactgatga gatgatcaaa gcgcaggtac
     1321 ttgccctgct tgaaaaacat caactgcgct atacggtgga ttggtggctt tccgggcagc
     1381 catttttgac cgcgcgcggt aaactggtgg atgcggtcgt taacgcggtt gagcactata
     1441 atgaaattaa accgcagcta ctgaccacag gcggaacgtc cgacgggcgc tttattgctc
     1501 gcatgggggc gcaggtggtg gaactcgggc cggtcaatgc c
//
LOCUS       DAZSNM010000409         1532 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZSNM010000409 DAZSNM010000000
VERSION     DAZSNM010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.416"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..291
                     /locus_tag="ACHW42_004363"
                     /pseudo
     CDS             1..291
                     /locus_tag="ACHW42_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            293..659
                     /locus_tag="ACHW42_004364"
                     /pseudo
     CDS             293..659
                     /locus_tag="ACHW42_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            662..1060
                     /locus_tag="ACHW42_004365"
                     /pseudo
     CDS             662..1060
                     /locus_tag="ACHW42_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
BASE COUNT          379 a          380 c          461 g          312 t
ORIGIN      
        1 tcaacgtatt accactgtca gcaacagcga catcatcatg ataaacgcag tgcccgtgcg
       61 cagcgcgatg actggctgaa gaaagagata ctgcgcgtat acgatgagaa tcatcaggta
      121 tacgctgtgc gtaaagtctg gcatcagttg ttacgggaag gtatcagagt ggccagatgc
      181 actgtggcac gtctcatggc ggttatgggg cttgccggtg ttctccgggg taaaaaggtc
      241 cacactaccg tcagccggaa agccgttgcc gcaggcgacc gcgtaaaccg tcatcaaggc
      301 aatgtgccgc gtactccggg tggcccgcag cggctggtat acgtggtgtc agcggcggac
      361 aaggataaac acacgtccgc agttccgtca gcactgcgac agcgttgtcc gcaaggcttt
      421 tacccagtcc agcgttacgg tgccccacgc ctgacggatg aactgtgtgc tctggttacc
      481 actttaacgt aaaaaccgtg gcagccagtc tgcgccgtca ggaactgagc gcaaaagcct
      541 cccagaagtt cagcccgatc agttaccgtg cacacggcct gcctgtatca gaaaatctgc
      601 tgacgcagga tttttacgcc agtggcccga accagaagtg ggcgggagac atcacgtact
      661 attacagctc accgactgcg gggaaacacg gggcgccggg ttattgaagg tgacctgtcc
      721 agctacttcg acacagtaca tcaccggctg ctgatgaaag ccgtctgccg caggatcagt
      781 gatacacgtt ttatgagact gctgtggaaa acgccgtgtt gatgttggtc tcttccgggc
      841 ggtcagtgaa agtatgccac agggcggtgt gatctcgccg ttgctgtcga acatcatact
      901 gaatgagttc gaccagtatc tgaataaacg ctacctgagt ggaaaagcca gaaaggatcg
      961 ctggtactgg aatcacagta tccagcgagg ccgaagtact gctgtcaaag aaaactggca
     1021 atggaaacct gccgttgctt actgttgcta tgccgatgac tgtgtgccac gaaggcgcgt
     1081 cctggggacg tgagtccctg tgcagccatg cagcacaaga gatgagggaa ggccccttgg
     1141 tgtcggcgtg caggcgcagg caccaaacca tccactgctg ctgcatttaa gagatgtggt
     1201 ggtgagcgag atgggaaaag gtcggacgtg agccgatcag gtatggttcg aagagacgaa
     1261 cgaaagtgaa ccgctgatga tgtgtcgaaa gcatatagat gatgtcaaaa caatggtgga
     1321 gtcgttcctg tgggatatgt ttactgcctg cgcggcatcc ggcataaaag cggcgtgaag
     1381 tcgattcagg ctcttgtggg gaacgtggac ctgtcacccc gatgtgaagg gagaagcgca
     1441 agtggaagcc ccatgagcgc cagagtacca atgcggggta cagggacgga gcaccaagcg
     1501 attgcgcagt aaacatgctt gacaacttaa tt
//
LOCUS       DAZSNM010000410         1522 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZSNM010000410 DAZSNM010000000
VERSION     DAZSNM010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.417"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(2..77)
                     /locus_tag="ACHW42_004366"
     tRNA            complement(2..77)
                     /locus_tag="ACHW42_004366"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Ile,seq:cat)
     gene            complement(254..943)
                     /locus_tag="ACHW42_004367"
     CDS             complement(254..943)
                     /locus_tag="ACHW42_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFR8945891.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(940..1299)
                     /locus_tag="ACHW42_004368"
     CDS             complement(940..1299)
                     /locus_tag="ACHW42_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR8945892.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(1299..1487)
                     /locus_tag="ACHW42_004369"
     CDS             complement(1299..1487)
                     /locus_tag="ACHW42_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945893.1"
                     /translation="MPFYFRKECPLNSGYLNMVPALRELMQAKHRAQKAVDELVDCVA
                     ELETRVGKFEKLVQEVLR"
BASE COUNT          374 a          428 c          314 g          406 t
ORIGIN      
        1 ttggtggccc ttgctggact tgaaccagcg acctggcgat tatgagtcgc tcgctctcac
       61 cactgagcta aagggccgga ggcagaataa taaccatatg tcatcacatc tgcaaactca
      121 tctgaccacc agcacgttta acgtcctgtg ccatttttca ggcataaaaa aaccgctcgg
      181 cggcgggttt tgcttacttt accatcgcgt acaaaatcgg caaagtatca gatttgcatg
      241 agatatatgc cttttaatct acttttgcaa tactttgctt tgaaaatgcc gctttttgtt
      301 ctgaacgtgt tctcatcaca aacaataaag cctcactatc cagtcggtga aaaatgtgtt
      361 tcattacaac ccagtgacca gtaaatgttt tggaccagtt tttggttgtt actcccgcca
      421 gtaatgccag ctcctgatat tcataacctt ccccaccaaa aagttctgct tttaccgcct
      481 gcgccgccag ccagattaat tttttcaggc gttcctgtgt tttctctgca attttcctag
      541 tgccagaatg agccttaaac tcttcccacg cccagtgggt tatggtgatc tgatactccc
      601 agcaaatact tccgctgtaa caccacaaca accaggcttt atagtgctct tcaagagaca
      661 gaacagcccg ccgccacgat gatgtcgaaa acgcaacctg actgaccaga ggaattgacg
      721 cccccttcgc cagtgattgt tttcccggga ttggtggatt atcccgcgtt atcatttttc
      781 cggtcacctc atcgcggtac cggatttttt tacgcctgta acgccctgta tcgaacatgg
      841 cattctcctg ccaggcttca agctgacctt ttgtcgcccc actcaaatca gcggtggcga
      901 taatgagctg ctcacgaaca aactgtaaat actggttatt catgcacact ccagctctgt
      961 gatttttatc cccaaccgac caccaggaac aggcagtccg cgcacaatat tggtttcatc
     1021 aaactgctcg tcgtcgataa gcaaccccgc atgtgtcagt gcatccagtg gtgctttcag
     1081 gatattgtcc aggtcacgac gccgcttatc cggtggctct gcaataatct ttatcgccag
     1141 tcttccggac aggcttaatt tcagtcgctg ctgacggaca ataagcgcca catcccggcg
     1201 ataacgctca ccggcttttg atacaaaata tgtgctgcca cgacgtcgcc agtaagtatt
     1261 caccgtcggc ggataaggta aaataaactc atggcgcatc agcgcagcac ctcctgcacc
     1321 agcttttcaa actttccgac tctggtttcc agctctgcca cacagtccac cagctcatct
     1381 actgctttct gtgcgcgatg cttcgcctgc atcagttccc gaagcgcggg taccatattg
     1441 aggtagcctg agtttaacgg acactccttc ctgaaataga atggcatcag aaggagccaa
     1501 taatgagcag aaaaacccaa cg
//
LOCUS       DAZSNM010000411         1510 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZSNM010000411 DAZSNM010000000
VERSION     DAZSNM010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.418"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            277..681
                     /gene="stpA"
                     /locus_tag="ACHW42_004370"
     CDS             277..681
                     /gene="stpA"
                     /locus_tag="ACHW42_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR8945894.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(728..1252)
                     /gene="ygaP"
                     /locus_tag="ACHW42_004371"
     CDS             complement(728..1252)
                     /gene="ygaP"
                     /locus_tag="ACHW42_004371"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR8945895.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1262..>1510)
                     /locus_tag="ACHW42_004372"
     CDS             complement(1262..>1510)
                     /locus_tag="ACHW42_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFR8945896.1"
                     /translation="LKAMSHPKRLLILCMLSGSPGTSAGELTRITGLSASATSQHLAR
                     MRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          413 a          345 c          350 g          402 t
ORIGIN      
        1 tattctgtta tattgcattt ttattcactt cggttttgtc aggcttgcgg agttagcgag
       61 cagagagcgc cactctgttc accaataacc taaattactc atcaaaatgg ctaatatata
      121 atcttgaaat tattctgaga gtttcagaaa ataacagctg aaataatctc gcgcaggact
      181 gtaaatagat taaattttgt ggaaatataa taagtgatcg cttacactac gcgacgaaat
      241 acttttttgt tttggcgtta aaaggttttc tttattatgt ccgtaatgtt acaaagttta
      301 aataacattc gcaccctccg tgcgatggct cgcgaattct ccattgacgt tcttgaagaa
      361 atgctcgaaa aattcagggt tgtcactaaa gaaagacgtg aagaagaaga acagcagcag
      421 cgtgaactgg ctgagcgtca ggaaaaaatt agcacctggc tggagctgat gaaagctgac
      481 ggaattaacc cggaagagtt attgggtaat agctctgctg ctgcaccacg tgctggtaaa
      541 aaacgccagc cgcgtccggc gaaatataaa ttcactgatg ttaacggtga aactaaaacc
      601 tggaccggtc agggccgtac accgaagcca attgctcagg cgctggcaga aggtaaatct
      661 ctcgacgatt tcctgatcta attactgagg ccggatgtcg ctgctagggc gcgtccggcg
      721 tcgcttctca agctcgttgg ttccacggca tcttatctaa caaccttgcc attccacaaa
      781 aaccgctgat tcctgcaaac agtaacccgg caccaacaaa gccacttaat aagaagaaac
      841 cgctatttac ggtataaccc agtacaacgc cgtttaatat taaaccaccc gcagcgatct
      901 gcacctggcg cattaacggc aagggttgag atttatttac cgctactggc aatccagctc
      961 ttttccagcc atcaatccca tcttcgagta aaaatatttc tgcgggggcg gcaatcgctg
     1021 ctaatttatc ggcgttatta ctggtgcgtt tacctgcctg gcagtggaat ataatttgct
     1081 cgcgacgtaa tttaggtgga agacctgact gttccagcac ggataatgga gccagatctg
     1141 cttcaggaat atgttcacga agatattcat cagcgtcacg aatatcgatt aactttgcgc
     1201 cgcgtgcgat taattcttgt gcatcatgcg gcgaaatggt tgtcaaagcc atagtgactc
     1261 cttacggaca atagacattt ttcagggtgg cgataatggc atttaccgcc tcatttttaa
     1321 tggaatatag aatgcgttgg gcatcccgtt ggctgttgat aagcccttcg tcccgcatac
     1381 gagcgagatg ctgtgatgtc gcagaggcgc tcagtccggt aatgcgcgtc agctctcccg
     1441 cgctggtgcc gggggaaccg ctaagcatgc acagaatcag caaccgttta gggtggctca
     1501 ttgctttcaa
//
LOCUS       DAZSNM010000412         1499 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZSNM010000412 DAZSNM010000000
VERSION     DAZSNM010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.419"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            119..379
                     /locus_tag="ACHW42_004373"
     CDS             119..379
                     /locus_tag="ACHW42_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR8945897.1"
                     /translation="MAWARHYQQIVREEKETELADDLEKGLPQHLFESLCIDHLQRHG
                     ANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            385..1482
                     /locus_tag="ACHW42_004374"
     CDS             385..1482
                     /locus_tag="ACHW42_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR8945898.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          454 a          340 c          365 g          340 t
ORIGIN      
        1 cgttgggttt ttctgctcat tattagctcc ttctgatgcc attctatttc aggaaggagt
       61 gtccgttaaa ctcaggctac ctcagagcgc ttatccgatt tttgatcgta ttgaagaaat
      121 ggcatgggct cgtcactacc agcagatcgt tcgtgaagaa aaagaaacgg aactggcgga
      181 cgacctggaa aaaggtctgc ctcagcacct gtttgaatcg ctctgcatcg accatttgca
      241 acgccacggt gccaataaac aggcaatcag ccacgcattt gatgatgatg ttgagtttca
      301 ggagcgcatg gcagaacaca tccggtacat ggctgaaacc attgcccacc accaggttga
      361 tattgatttg gaggactaaa atctatggcg ctttttcaac gagcaataaa cacacaggcc
      421 tatctaaaag caggaattat ggggtttgct ggcgacggca aaacctacac agcaagcgaa
      481 ctggctattg gtctggtaat gcttatgcgg caacggggaa ttgaagctgg taaccgcccg
      541 gtaatgtttc tggacacaga aacaggctct gactgggtta aaccccgctt tgatgcggaa
      601 aatatcgaat tatttacggc aaaaacccgc tcgtttgttg atctgcttga agccattaat
      661 gaagcggaaa gcagcggttc cgtaatgatc atcgactcca tcagtcactt ctggaccgga
      721 ttatgcgacg agtatgccag acgccgaaac cgtaaacgag gactggaatt ttcagactgg
      781 gcgtggctca aacaggagtg gcgacgcttt accgatcgat ttgttaacag tcaggcgcac
      841 attattatgt gcgggcgtgc aggatatgag tatgactttt tcgaaggtga tgatggcaaa
      901 cgccagttgg caaaaaccgg tataaaaatg aaagcagaaa cagaaaccgg ttatgaaccc
      961 tccatccgga tccagatgga gaagcaaatg aacatagaaa ccggtcaggt gtggcgcacc
     1021 gccagaatac taaaagatcg ctccacccgt attgatggac aggtattttc aaacccaaca
     1081 tttaaaaact ttcttcctca tatcgagtct cttaacctgg gaggtgaaca ccccggcatc
     1141 gatacctcac gggataacag tgaattattt gccaacgacg gtacaccgac atggctaaaa
     1201 gaaaagcgtg caaaagaaat cgcacttgat gaaattatcg agttgctcaa caagcaccat
     1261 gggggcacca gtaacgacgc caaacgcgca aaagctgatt tgctggaaaa aacattcacc
     1321 tcccgctcat gggaaagaat caaaggtatg gactggccga caatcaaagc tggtcgtaat
     1381 gcactatgga ttgaactgga aggcgttgaa tatgcattcc cggatccaca aacacaaaac
     1441 gaaacgcaac aagcaggata cgatgagaat attccggtgt aaaaccaagt gcattttgg
//
LOCUS       DAZSNM010000413         1485 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZSNM010000413 DAZSNM010000000
VERSION     DAZSNM010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.420"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            298..>1485
                     /locus_tag="ACHW42_004375"
     CDS             298..>1485
                     /locus_tag="ACHW42_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR8945899.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
BASE COUNT          411 a          325 c          348 g          401 t
ORIGIN      
        1 tagtatcgat atcaaatcag cctgcggccg cgataacttt cgtaacattc tgccagcggc
       61 tttgttgaat aaatcaggtt caagataatc gtctctgcat caggttagag tttcaggcaa
      121 tacatcctct ttctggcata cctgccactc tcgcaccatg gtcatagcct ttacgatctg
      181 catactcacc agaccagagc ccgctccctt ttaggctgag gaatccccag aaaagaagac
      241 aggcatagag ttttgtgatc tactgattgc gctaacaaat tcaatgagat cacctctatg
      301 cctgcccgta aagtatgcca gacttttttc cgtaatgctt tagcttccac gcatcagtat
      361 cgacaaaatg ccattattga ttctgctgct gctttagtcg gtggcgcatc tctttctctt
      421 accagcattg gccggcattt acccggtcct gctcgcgtga aagataaaat aaaacgcgtt
      481 gactgtctgt tgggtaataa acgacttcat aatgatatcc ctctgatatt caaaaatatt
      541 acatccatga tgacaaataa actgtcgtgg tgtgttatcg ctgttgactg gagcggttac
      601 ccttttcagg aatatcatgt ccttcgtgcc agcctcctct gcgacggtcg ctctatcccc
      661 ctgatgagtc aggtcttccc gtcaaaaaaa aataacgagg cggtggaaat cgcttttctg
      721 gatgcgcttt ccggggccat ttctccccgg actcgtgttg ttattgttac tgatgctggt
      781 tttcaaagtg catggttccg tcatatcaaa tcacagggat gggattttat tggtcgtatc
      841 aggggggtag tgaagttcag gctggatagt gacaaagata agtggctgga cataaaaatg
      901 tgcaggggga gttcagaggc aaaatacctg gggacgggaa ccctggcccg gaaaaaacgc
      961 tcgcagtgtg aaggacattt ttatctttat aaacattcac ctaaaggcag aaaaagcagg
     1021 cgtgccagag ggaggccggg cttaccaaca acggaaaaag aacaaaaagc cgcaggcaga
     1081 gaaccatggt taatattcag caatacagct gagtttaatg ccaaaaaaat catgaaactg
     1141 tacagtcgaa gaatgcagat agagcaaaac tttcgggatg aaaaaagtga acgctttggt
     1201 tttggccttc gggcgagcag aagtcggagg ggagagcgtt ttctggtatt aagtctactg
     1261 gttacactgg ccagtatcgt actctggcta cttggttatc attttgaaaa taaaggattt
     1321 catttaaagt atcaggctaa tagtctcaaa aaacgaagag tattgtcttt tctgacgctg
     1381 gctgaaaacg tcctgagatt taaccctgaa cttctacgac gagcacaacc tgaaaagata
     1441 ttgtgccggt tggccagcac ttaccgaagt atggtgctgg cttac
//
LOCUS       DAZSNM010000414         1483 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZSNM010000414 DAZSNM010000000
VERSION     DAZSNM010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.421"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(3..656)
                     /gene="ribB"
                     /locus_tag="ACHW42_004376"
     CDS             complement(3..656)
                     /gene="ribB"
                     /locus_tag="ACHW42_004376"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR8945900.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(761..909)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1031..1321
                     /gene="ubiK"
                     /locus_tag="ACHW42_004377"
     CDS             1031..1321
                     /gene="ubiK"
                     /locus_tag="ACHW42_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR8945901.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT          412 a          385 c          327 g          359 t
ORIGIN      
        1 tttcagctgg ctttacgctc atgtgcctga cggtatgcca ccaggtcttc aatagtcacg
       61 agcgccatat tgtgtttatt ggcaaactca atgcactctg gtgcacgcgc catcgtgcca
      121 tcgtcattag tcagctcaca cagcacacca gccggtttaa agcctgccag cgtcatcaaa
      181 tcaatagtcg cttcagtatg accgccacgc gtcagtacac cacctgcctg cgcgcgaagt
      241 gggaaaacgt ggcccgggcg attcagatca gaaggttttg cgccatcggc aatcgctgcg
      301 cgaacggtcg taatacgatc agcggcagaa acaccggtag tcacaccttc agccgcttca
      361 atggtcacgg taaaaccggt gccataggcg ctggtgttat tttctaccat cattggcaga
      421 tcaagttgtt tacggcgatc ttcagtgatg cacaggcaaa caataccgct accgtggcga
      481 atggtcagcg ccatctgctc aacggtcatg gtttctgccg ggaagatcat atcgccttcg
      541 ttttcacggt cttcatcatc aagcaccatt acaccgcgtc cttcacgcag cgcagccagt
      601 gcattttcaa cacgttcgaa aggcgtacca aaagaggaaa gtagcgtctg attcatggta
      661 aaaaaaacct cactaaaatt atggttacca gaatcagggc agtcttagga gtggcggcat
      721 atagccaaaa taacgtgagc gggtccatgc ccgacagaat cgttactctc tcccatccgg
      781 actctaaccg tcggccccgg aattacaccg gatctgctgt cctttgagtt cgcacccaaa
      841 gcgctcgcgg gctttcaact gagttgattt accgccggtg gggaatttcg ccccgccctg
      901 agaataagcg gattcactat aacgctaatg attagcggca gcaacgcata gcttcacata
      961 attctggttt atgacttacc cttatcgcac tacaatggta ctcaacacct atcagtacag
     1021 ggaaactaca atgattgacc cgaaaaaaat tgagcaaatc gctcgccagg ttcacgaatc
     1081 aatgcctaaa ggaatcaggg agttcgggga agatgtggag aaaaaaatcc gccaaaccct
     1141 acaagctcag ctgacgcgcc tcgaccttgt aagccgtgaa gagttcgacg tccaaacgca
     1201 agtcctgtta cggactcgtg agaaactggc gctgcttgaa caaagaatca gcgaactgga
     1261 aaatcgcagt actgagatca aaaagcagcc agatccagag actctccccc caacgctgta
     1321 aacatttgtc tttaatgcac tgtaaattat gaattaatcc tctaatttac agtgttttct
     1381 attacattat cgcttaatcc cattcagagt atcatcacaa aaatgaatga ttgcaattga
     1441 aaatataatt gttatgtatt ctttaagttt tacttaatcg ata
//
LOCUS       DAZSNM010000415         1441 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZSNM010000415 DAZSNM010000000
VERSION     DAZSNM010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.422"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            400..1149
                     /gene="phoC"
                     /locus_tag="ACHW42_004378"
     CDS             400..1149
                     /gene="phoC"
                     /locus_tag="ACHW42_004378"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase PhoC"
                     /protein_id="HFR8945902.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1204..1308)
                     /locus_tag="ACHW42_004379"
                     /pseudo
     CDS             complement(1204..1308)
                     /locus_tag="ACHW42_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1319..>1441)
                     /locus_tag="ACHW42_004380"
     CDS             complement(1319..>1441)
                     /locus_tag="ACHW42_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFR8945903.1"
                     /translation="CFGRLKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          401 a          299 c          294 g          447 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcactatc gccaggtaat
     1381 ttctcgccgt tttgtcgtaa cgcgtggcga tgcggcgata ttctttcagc cgcccaaagc
     1441 a
//
LOCUS       DAZSNM010000416         1437 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZSNM010000416 DAZSNM010000000
VERSION     DAZSNM010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.423"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..373
                     /gene="tnpA"
                     /locus_tag="ACHW42_004381"
     CDS             <1..373
                     /gene="tnpA"
                     /locus_tag="ACHW42_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFR8945904.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
     gene            373..720
                     /gene="tnpB"
                     /locus_tag="ACHW42_004382"
     CDS             373..720
                     /gene="tnpB"
                     /locus_tag="ACHW42_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR8945905.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            740..>1437
                     /locus_tag="ACHW42_004383"
     CDS             740..>1437
                     /locus_tag="ACHW42_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFR8945906.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPVLPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVAGKYADYLPLYRQSEIYRRQ
                     GVELSRATLGRWTGA"
BASE COUNT          341 a          400 c          398 g          298 t
ORIGIN      
        1 gcggcctaat tttccttatg aatttaaaat cgctttagtg gagcagtcac tgcagcccgg
       61 agcctgtgtg gcgcagatcg cccgtgaaaa cggaatcaac gataacctgc tcttcaactg
      121 gcgccatcaa taccggaaag gtggcctgct gccttccgga aaaaatatgc ctgcactgct
      181 tcccgtgacg ttaacgccgg agccggataa taaaatcccg gctccggcac aggaaccaga
      241 gcagataaat acaccgtccg acagtctgtg ctgtgagctg gttctgccgg ccggaactct
      301 caggcttaaa ggtaaactga cgccggcgtt attacagata cttatccgcg aaataaaagg
      361 gagcagccac tgatgatatc tttccctgca ggttcgcgta tctggctggt tgcaggtatc
      421 accgatatgc gaaatggctt taacggcctg gtctcaaaag ttcagaacgt cctgaaggat
      481 gacccgttct ccgggcatct gttcatcttc cgcggacgcc ggggtgacca gataaaagtg
      541 ttgtgggctg acagtgacgg actgtgcctc tttaccagac gcctggagcg gggccgcttc
      601 gtctggccgg tcacccgtga cggcaaggtg caccttactc cggcccagtt atccatgctt
      661 cttgagggca tcgactggaa acacccgaaa cgaacggaac gcgctggcat ccgcatataa
      721 cccgttgtaa agtgaggata tggacacctc acttgctcat gagaatgccc gcctgcgggc
      781 actgttgcag acgcaacagg acaccatccg ccagatggcg aaatacaacc gcctgctctc
      841 acagcgggtg gcggcttatg cttccgaaat caaccggctg aaggcgctgg ttgcgaaact
      901 gcagcgcatg cagttcggta aaagctcaga aaaacttcgc gcaaaaaccg aacggcagat
      961 actggaagca caggagcgaa tcagcgcact tcaggaagaa atggcggaaa cgctgggtga
     1021 gcaatatgac ccggtactgc catctcccct gcgccagtct tcagcccgta aaccgttacc
     1081 agcctcactt ccccgtgaaa cccgggtcat ccgtccggaa gaggaatgct gcccggcctg
     1141 tggtggcgaa ctcagttctc tgggatgtga tgtgtcagag cagctggagc ttatcagcag
     1201 cgcctttaag gttatcgaaa cacaacgtcc gaaactggcc tgttgccggt gtgaccatat
     1261 cgtgcaggca ccggtgcctt caaaacccat tgcacgcagt tatgccggag cggggcttct
     1321 ggcccatgtt gttgctggaa aatatgcaga ctatctgccg ttataccgcc agtcagaaat
     1381 ataccgtcgt cagggcgtgg agctgagccg tgccacactg gggcgctgga caggtgc
//
LOCUS       DAZSNM010000417         1431 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZSNM010000417 DAZSNM010000000
VERSION     DAZSNM010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.424"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            39..>1431
                     /gene="ccp"
                     /locus_tag="ACHW42_004384"
     CDS             39..>1431
                     /gene="ccp"
                     /locus_tag="ACHW42_004384"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR8945907.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDK"
BASE COUNT          383 a          302 c          394 g          352 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa c
//
LOCUS       DAZSNM010000418         1404 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZSNM010000418 DAZSNM010000000
VERSION     DAZSNM010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.425"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..167
                     /locus_tag="ACHW42_004385"
     CDS             <1..167
                     /locus_tag="ACHW42_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFR8945908.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="ACHW42_004386"
     CDS             152..790
                     /locus_tag="ACHW42_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFR8945909.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1404
                     /locus_tag="ACHW42_004387"
     CDS             1018..>1404
                     /locus_tag="ACHW42_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945910.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          393 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata
     1201 tttattaata aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa
     1261 agaagagata tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta
     1321 tttgatcatg acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagt
//
LOCUS       DAZSNM010000419         1404 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZSNM010000419 DAZSNM010000000
VERSION     DAZSNM010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.426"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHW42_004388"
                     /pseudo
     CDS             complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHW42_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
     gene            complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHW42_004389"
     CDS             complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHW42_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR8945911.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          389 a          318 c          292 g          405 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtgtttttga ttcaaaggaa tagt
//
LOCUS       DAZSNM010000420         1385 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZSNM010000420 DAZSNM010000000
VERSION     DAZSNM010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.427"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(8..775)
                     /gene="ssuB"
                     /locus_tag="ACHW42_004390"
     CDS             complement(8..775)
                     /gene="ssuB"
                     /locus_tag="ACHW42_004390"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR8945912.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(772..>1385)
                     /gene="ssuC"
                     /locus_tag="ACHW42_004391"
     CDS             complement(772..>1385)
                     /gene="ssuC"
                     /locus_tag="ACHW42_004391"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR8945913.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT          353 a          437 c          322 g          273 t
ORIGIN      
        1 cggcatatta accatgacgc cgaatcagtt gttcagaatg cccacgccgc attacccgtt
       61 gcaatacttc cgcttccagc tccgctaatc gcaccgaccc taatcgacgc ggacggggga
      121 tatccactgt caaatccaga ctaatttttc cctcttcaat taacaacacc cggtcagcca
      181 ttgctaccgc ttcgctcaca tcatgcgtca ccaacagtac ggtaaagccg tgctgctgcc
      241 aaagtgacac aatcaaatcc tgcatctcga gtcgcgttaa ggcgtccagt gccccgagcg
      301 gttcatcaag caacaataaa cccggtcgat gaatcaacgc tcttgccagc gccacacgct
      361 gtttctgccc gccagaaagt gcggcaggcc attccccggc gcgattctcc agtcctgccg
      421 cagccaacgc ccgacgcgcg gcatcgcgcc actgaccttt aaggcctaac ccaacgttat
      481 caatcaccga tttccacggc agcagacgcg catcctgaaa catcattcgc gtatcatcct
      541 gaatttcagc cagcggtgtg gtgcccgcta acacatcgcc tgcggttggc gtttccagac
      601 cggccagcag gtgcagcagg gtacttttac caccaccgct gcggcccacc accgccacaa
      661 actgacctgc cggaatatgt aaatccagtt ggttcaggac gatattttcc gcgtaatgtt
      721 tgcttactgc attgagcaac aatggtgtgc cctggttcag acgagcagta ttcatatcgt
      781 ggcctccttc aaatgataag ccgggttcca gcgcaaccag aggcgctcta acagctgcgc
      841 gctgacgtca gcaagtttgc cgagcagggc gtaaagaata atagcgacta ccaccacgtc
      901 tgtttgcaaa aactctcgcg cattcatcgc cagataacca atgccggaat tggcagaaat
      961 ggtttcggca acaatcagcg tcagccacat caggcccaac gcaaaacgca cgccgaccat
     1021 aattgagggc agggcatcgg gcaggatcac atggataaac agcggtatgc cggataatcc
     1081 atagctacgc gccatctcca ccagcccgcg atcgatatta cggatgccat gccaggtgtt
     1141 gatataaatg gggaacagcg taccgagcgc caccagaaag attttcgcgg attcatcaat
     1201 gccaaaccac aaaatcacca gcgggatcag cgccagatgc ggcacgttgc gcaacatctg
     1261 aattgaggta tccagcagcc tttctcccca gcgcgacagt ccgctaatca gtcccagaat
     1321 caggcccagc gatccgccaa ttgaaaagcc aatcagcgcc cgccaggaac tgatcgccag
     1381 atgct
//
LOCUS       DAZSNM010000421         1380 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZSNM010000421 DAZSNM010000000
VERSION     DAZSNM010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.428"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            428..597
                     /locus_tag="ACHW42_004392"
                     /pseudo
     CDS             428..597
                     /locus_tag="ACHW42_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            743..>1380
                     /locus_tag="ACHW42_004393"
     CDS             743..>1380
                     /locus_tag="ACHW42_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR8945914.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTL"
BASE COUNT          340 a          319 c          421 g          300 t
ORIGIN      
        1 caacacaaag gcaactaata aagatacaga taaagatcta aaaacagatc taacccatcc
       61 caaacccttc ccttccggaa gggagtttcg ggattttgtg gctggagtgc ttgaggggag
      121 attatctggc ggtactgcag cggaattttg taattctgcg gtggttgcgt tgcaggctgc
      181 tggcctggat gtctgtcgtg agtatccggt gccagagcgt ggtgacggtt gcggagggcg
      241 gattgatatc gtcgtgactg acaggaacgg tgtccggtgt gggatcgagc ttgaccgaaa
      301 ttctccgcga cagaaatcac tgctcaaaat cggtgctgtt gaaaccggga tatgtgtctt
      361 gcggcgcagt gatatcgcaa ggcacaccga gcagggaatt ctggttatcg gtggggctgt
      421 tcgccagaaa aaatttgacc cgttgtcagt tgatctgccc gactggttgt cagaaacact
      481 ctggcatgag tgggtccagt tcaggcaggc attgcgaaaa ccgattcgaa cggagctggg
      541 cgctaacggg gcgatacggg aactggaaaa attccgtcag cagggtttta cacctgagca
      601 ggtgattcga cacagcatcg ccaatgaata ccagggtctg ttcgcgccga aaggtgttcg
      661 gcctgagacg ttgctccgac aggttaacac cgtctcgttg ccggacagtg cgatcccgcc
      721 aggcttcagg gggtaacaga ccatgaaaaa tattgcgaca ggaggcgttc tggagcgtat
      781 ccgcagactg accccaccac atgtaaccgc cccattcaga acggttgcgg agtggcgcga
      841 gtggcaactt gctgaaggcc agaaacgttg cgaggagatc aaccgcctga atcgtcagtt
      901 gcgggtggaa aaaatcctga accgctccgg catccagccg ttgcaccgga agtgttcgtt
      961 tgcgaattac cgggcgcaga acgacggtca gcgacatgca ctgagtcagg cgaaatccat
     1021 agctgacgaa ctgatgacag gctgtacgaa ttttgtgttc agcggtaagc ccggcaccgg
     1081 aaaaaatcat cttgcagccg cgattggcaa ccggctgatg gcgaaggggc gtagcgtgat
     1141 tatcgtcacc gtgtccgatg tcatgagcgt gttgcatgag agctacgaca acggcaaatc
     1201 cggtgaaaaa tttttacagg agctttgtgg tgttgacctg ctggtcctgg atgaaattgg
     1261 catgcagcgg gatacgaaaa acgagcaggt ggtactgaac cagattgttg atcgccggac
     1321 ggcatcgtta cgcggtgtgg ggatgctgac aaatattaac catgcagcga tgaatacact
//
LOCUS       DAZSNM010000422         1376 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZSNM010000422 DAZSNM010000000
VERSION     DAZSNM010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.429"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(32..388)
                     /locus_tag="ACHW42_004394"
                     /pseudo
     CDS             complement(32..388)
                     /locus_tag="ACHW42_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            468..>1376
                     /locus_tag="ACHW42_004395"
     CDS             468..>1376
                     /locus_tag="ACHW42_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HFR8945915.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          375 a          306 c          340 g          355 t
ORIGIN      
        1 gccatcagtc gttgatgttg cagttccatc atcacgccac tcctctgcag aatgagtcgt
       61 agatggagag tggatgatgc tggggggggt tgtcgaagct caccagattt tcaccaggat
      121 gcacgtcata ctcttttttc tccggaggca gtgccagcat ggactgctgc tcttcgagcc
      181 agcgatcaca gggacgggcc tggattgttt catgctttcg ttggttagcg acatcgtgca
      241 gccagcgcag accgtggcgg ttggctgttt caacatcgac agtgctcccc atcgggcgca
      301 ggcgagtcat tagtgggatg taaaaactgt tacgggtgta ctgcaccatc cgttccacct
      361 tacctttagt ctgtgccctg aagggctgag gaatccccag aaaacgagac aaacatagca
      421 ttatgtgatc tactgatttg tgccaacaat tcaatgagat cactgctatg cctgttcata
      481 aagtatgtca gaatttcttc cgggatgcct tagcaccatt tcatcagtac cgacaaaatg
      541 ccctgatgga tgccacaatg gcgttaatca acggagcatc tctgacgcag accagtatcg
      601 gacgtttttt gccgggtaat gcacaagtta agaataagat caaacgtatt gatcgcctca
      661 tgggtaatga ggcgctccat cgcgatattc caatgatttt caggaatatc acttcaatgc
      721 tgacacggca gttatcttta tgtgtaatag ccgtagactg gagtggctat ccctcccagg
      781 agcatcatgt tcttcgtgcc agccttctgt gtgacgggcg ttccattccg ttattgagta
      841 aggttgttcc ttctgaaaaa caaaataatc cattgataca acatgatttc cttgattccc
      901 ttgctcaatc cctgcccccc gatgccaggg ttattattgt cacggatgct ggatttcaga
      961 gcgcatggtt tcaccatatt acttctctgg gctgggattt tattggtcgg atcagaaata
     1021 acgttcagta ttgcctcgat aacgcgcctg aaagatggtt aaaagtgtcg gatagccctg
     1081 aatgtaaaac accggaatat atgggggctg gaaggctggt taaagaaagg aaaaaaagca
     1141 tccgggggca tttttacacc tataagaaat cggcaaaagg gcgaaaaaag aaacgttcga
     1201 aaggtcagag tggtttaaac aaaacagaca aagaacagag caaatcagca aaagaagcct
     1261 ggttgatctt cagcagtaca aatgacttca gagcgcgaga aatcatcaag ttatacagtc
     1321 ggcgaatgca gatagagcaa aactttcgtg atgaaaagaa cgggcgcttc ggattt
//
LOCUS       DAZSNM010000423         1359 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZSNM010000423 DAZSNM010000000
VERSION     DAZSNM010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.430"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(393..>1359)
                     /locus_tag="ACHW42_004396"
     CDS             complement(393..>1359)
                     /locus_tag="ACHW42_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR8945916.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGSDNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          415 a          284 c          288 g          372 t
ORIGIN      
        1 aaggaaaacg ttaacaacta tcaaaaaacg ctcattttaa aaagttatta tgttctccga
       61 tagtgtgtga agtaatattt ttgtgatgtt ttagatgtgt aataatgaac caggttaaag
      121 tgtatttaaa aatgtgttgt attagcgact gaaatctgcc ttttaaaaag gaggagggta
      181 ctgagacaaa gagtcattgc ttctccgata aaatgtcaga gaagcatgat tacgtggtta
      241 ttgtctgttg atgacttgtt gaccgagccg tcatcattct caatgcatta gttaaatcat
      301 ttgtgatttt taaggattat cctgtctatg attttctact tagaaggata tggcactaac
      361 tatgcatctt atagtttact gctggcatct atttaaaata gcaattgagt caaatagccc
      421 atcaagatag ccatacctaa tataaccgtc aggaatgcag ctatcatcgg cattctgaac
      481 attgatttaa gtaatatcac ttccgtcagg cttgcgccgg cactgccgat gattaatgcc
      541 attaatgctc ccagacccat tccttttgtc atcagaacag atgccagagg gataaccgcc
      601 tcagcccgga tatacagcgg aataccgaca acggcgctta atgggatggc gaagggatta
      661 tctgatcctg catgagcggc aatccacgct gagggaataa agccataaat aaaagatcct
      721 attaatactc ctaacaaaag atatggcagt acatctttaa actgttgcaa cgcatctttg
      781 gtggctagtt ttatgggatt aatacttact acagctttgg tactgcaaca ggtaactacg
      841 gggttctcaa tggctttagc cgctggacta caacaactca cttttatggg cgtatatgtc
      901 gttcccggcg aagtttttgc tggagcacaa caatttgctg atgagctttt actggcaatg
      961 atatgacgtt caaaccccag agaatccagg ataatactgg caagaacgga gacgccggct
     1021 gcgataatcg cgtacaacaa ggtgactttc caaccaaagg tcatccacat tagtccgacg
     1081 ataatgggat taagtaatgg tgaaacaaat aaaaaagtga gagtcggacc aaacccggct
     1141 ttcgctgaca acaatccacg taacatgggt attgtcgagc aactacagaa cggggtaacg
     1201 gctcccaaca gagcagccag gagatagcct ctcccttttc tggcccccat catctgctgg
     1261 attttatggt ctggcacctt ttgtcttatc aggctgacac cggcactaat cactataaac
     1321 agcaacgaaa gctcgacggc gagaaacaca aacatctct
//
LOCUS       DAZSNM010000424         1356 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZSNM010000424 DAZSNM010000000
VERSION     DAZSNM010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.431"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(3..485)
                     /locus_tag="ACHW42_004397"
     CDS             complement(3..485)
                     /locus_tag="ACHW42_004397"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8945917.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="ACHW42_004398"
     CDS             complement(476..778)
                     /locus_tag="ACHW42_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR8945918.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1050..1322
                     /locus_tag="ACHW42_004399"
     CDS             1050..1322
                     /locus_tag="ACHW42_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945919.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          398 a          342 c          288 g          328 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgcctg aaccacggca gcctggcggc tgcaaatcgg ggtgtggcag aatatgagct
     1021 ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac cagaaagtcc
     1081 acaccagaat ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttata
     1141 agtccgtatt tgcagacttt atcaccggtc atccctcctg cacggtctgt ttctgggaaa
     1201 ccttccacaa aatgtacccg gattcaccgt acgaatacgt cacgttctgc catacctgcc
     1261 gtcgttttga tttatacgaa acagaagcgg agatgaaggc ggatgacccg aagtggtggt
     1321 gattttcagc cccgaccgct gaaacgcctg ttcaca
//
LOCUS       DAZSNM010000425         1345 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZSNM010000425 DAZSNM010000000
VERSION     DAZSNM010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.432"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            143..883
                     /gene="phoN2"
                     /locus_tag="ACHW42_004400"
     CDS             143..883
                     /gene="phoN2"
                     /locus_tag="ACHW42_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFR8945920.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            956..1078
                     /locus_tag="ACHW42_004401"
     CDS             956..1078
                     /locus_tag="ACHW42_004401"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945921.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(1243..1329)
                     /locus_tag="ACHW42_004402"
     CDS             complement(1243..1329)
                     /locus_tag="ACHW42_004402"
                     /inference="COORDINATES: protein motif:HMM:NF017898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945922.1"
                     /translation="MLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT          397 a          287 c          266 g          395 t
ORIGIN      
        1 tgttattact ttaatataag actatttttt gtttttccat cactctgttc aaatttttcc
       61 gcatgacttg tgttttttgt aatacagctc gttttttaca gctgaccaaa atcatcaatt
      121 aattatgcta aggaaataaa ttatgaaaac caaaaacttt cttctttttt gtattgctac
      181 aaatatgatt tttatcccct cagcaaatgc tctgaaggca gaaggttttc tcactcaaca
      241 aacttcacca gacagtttgt caatacttcc gccgcctccg gcagagaatt cagtagtatt
      301 tcaggctgac aaagctcatt atgaattcgg ccgctcgctc cgggatgcta atcgtgtacg
      361 tctcgctagc gaagatgcat actacgagaa ttttggtctt gcattttcag atgcttatgg
      421 catggatatt tcaagggaaa ataccccaat cttatatcag ttgttaacac aagtactaca
      481 ggatagccat gattacgccg tgcgtaacgc caaagaatat tataaaagag ttcgtccatt
      541 cgttatttat aaagacgcaa cctgtacacc tgataaagat gagaaaatgg ctatcactgg
      601 ctcttatccc tctggtcatg catcctttgg ttgggcagta gcactgatac ttgcggagat
      661 taatcctcaa cgtaaagcgg aaatacttcg acgtggatat gagtttggag aaagtcgggt
      721 catctgcggt gcgcattggc aaagcgatgt agaggctggg cgtttaatgg gagcatcggt
      781 tgttgcagta cttcataata cacctgaatt taccaaaagc cttagcgaag ccaaaaaaga
      841 gtttgaagaa ttaaatactc ctaccaatga actgacccca taaagctgga cagcctgtat
      901 caggctatgg agggcccata gacaaatcta ccctatatga gcataggagg agtctatggg
      961 cacaccacgt tttacccctg aatttaaggg attactggaa aggctgggac atatccctcc
     1021 ggcagaagca gaaaaagctt attatgctgc catcggaaac gatgatctgg caacctgagt
     1081 tcacagataa aacattctct aggaaactcg gggcggttcc gttcaccaca tgcaatgtgg
     1141 tgttgcaggg gaacggtctg caccatcccc tatgtcgatc aatataacag aaatgacaac
     1201 ttcagattca gggcacaacc taaatatatt ttaggtcacc tctcaaatcg tttgcctgat
     1261 accgctccat tttttaataa aaaaatcaat catttttgaa tctttgcttt tcattgcatt
     1321 gtcaagcata gtgtccccac tattt
//
LOCUS       DAZSNM010000426         1319 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZSNM010000426 DAZSNM010000000
VERSION     DAZSNM010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.433"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..146)
                     /locus_tag="ACHW42_004403"
     CDS             complement(<1..146)
                     /locus_tag="ACHW42_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8945923.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGP"
     gene            204..1229
                     /locus_tag="ACHW42_004404"
                     /pseudo
     CDS             204..1229
                     /locus_tag="ACHW42_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            1202..>1319
                     /locus_tag="ACHW42_004405"
     CDS             1202..>1319
                     /locus_tag="ACHW42_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945924.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          362 a          324 c          306 g          327 t
ORIGIN      
        1 tccggaccgt acttctttat ccaggcataa aggctgtggg tggtgatatc gagacgtgtt
       61 gcaacgctgg caacagaata accgcgatca acaacctgtt tgactgcttc agttttaaac
      121 tcttcgggat aacgcttacc gctcatgggc acctctcttt aagccatctt aaatgactct
      181 gaggtgtctg ttaaacccgt ggcgattcac aattctcttg caccattaac tatgttcagt
      241 ccctgcgcga gttgtacacc gatgtcatcg aagagtttta ctgggttgcg ctcccgctta
      301 cgacgcaaaa ttccctttca caataccaac ccgaatggca gtgctgggaa cctgatgtcg
      361 aatgggtgcg tcagcccccg caagatgcga taaccgctcc tgactttttc tgcttttacc
      421 agccaggcat gaccttcgaa caatttgtac gtgagtttgc cgaatggttt tcacaaaaac
      481 gtccggcggc gatgatgatc ggcatccgtg cggatgagtc ctacaaccgt tttgtcgcca
      541 tcgccagttt aaataaacaa cgttttgccg acgataaacc atggactacg gccgcaccag
      601 gcggtcatag ctggtacatt taccccattt acgactggaa agtggctgat atctggacct
      661 gatatgcaaa tcatcaaaat ctctgcaacc cactgtataa cctaatgtat caggcaggcg
      721 ttcctctgcg tcatatgcga atttgcgaac cttttggccc ggagcaacga caaggattat
      781 ggctctgtca cgttatcgaa ccggatcgct gggctgctat gtgcgcacga gtcagcgggg
      841 taaaaagtgg cggcatttac gccggacatg acaatcattt ctatgggcat cggaaaatcc
      901 tcaaaccaga acatttagac tggcaagaat atgcattatt gctgctcaat agcatgccgg
      961 aaaaaacagc tgagcattac cgcaataaaa tcgccattta tttgcactgg tatcagaaaa
     1021 aaggcatcga ggttccacaa acccagcaag gggacattgg cgcgaaagat atcccctcct
     1081 ggcggcggat atgcaaagtt ttactcaata acgattactg gtgtcgggca ttatcattta
     1141 gtcctacgaa agcgaagaac tatcagcgtt ataacgaacg gataaaagga aaacgtcagg
     1201 aatgggggat actatgcaac aacgattaac gcaggattta acgcaatttc tcgccagctt
     1261 gccggaagat gatcgcatca aagcgattaa tgaaatccgc atggcgatcc atcaggtta
//
LOCUS       DAZSNM010000427         1311 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZSNM010000427 DAZSNM010000000
VERSION     DAZSNM010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.434"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..190)
                     /locus_tag="ACHW42_004406"
     CDS             complement(<1..190)
                     /locus_tag="ACHW42_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR8945925.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..>1311
                     /gene="minC"
                     /locus_tag="ACHW42_004407"
     CDS             710..>1311
                     /gene="minC"
                     /locus_tag="ACHW42_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR8945926.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGE"
BASE COUNT          386 a          293 c          315 g          317 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat a
//
LOCUS       DAZSNM010000428         1297 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZSNM010000428 DAZSNM010000000
VERSION     DAZSNM010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.435"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..257
                     /locus_tag="ACHW42_004408"
     CDS             <1..257
                     /locus_tag="ACHW42_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFR8945927.1"
                     /translation="EYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGMNRI
                     YLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            257..652
                     /locus_tag="ACHW42_004409"
     CDS             257..652
                     /locus_tag="ACHW42_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR8945928.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            835..1215
                     /locus_tag="ACHW42_004410"
     CDS             835..1215
                     /locus_tag="ACHW42_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFR8945929.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT          455 a          252 c          254 g          336 t
ORIGIN      
        1 atgaatatct ccagacatta ataaatgatg tagctgaaat aaaaaacatt tctcatgtaa
       61 tagacagaga agtgaaatct ctggcagaaa gcatcaggca ggaaatttca acattctcag
      121 gaatgaaccg aatctatctc actggcggag gagccgagct tatttaccct cacataaagc
      181 aatatttccc gaatctgaaa gtgaacaagg tggatgaacc tcagtttgca ttggtgaaag
      241 caatggtgca tgcgtaatgg aaagcagtga tccaaaaaaa agaaagaaag ttgtggcata
      301 cctgcacccg gcactttatc cgcaggacaa cctgacacaa caaacaatag actctctccc
      361 agtacaaatg cggggagatt tctacagaca atcccttatt tgcggagcag ctctttattc
      421 tgttgccccc cgactgttaa cgctaatcag cgtttttttc agtgagaaaa tcactgcaga
      481 aaatctggtg aaacttatag aacaaacaac aggatacaca tccacatcaa ttgatataag
      541 tgtgttaaaa aacatcatag aggcatcttc agaaaacaaa tcagagagca tcacttcaaa
      601 agatgatttc gaagaacaaa ccagacgcaa tctttctatg ttaaaaaaat aataaaagaa
      661 gagcaggttc agtcaattac ctgctcttct tttatacata ctaaaaaatc agatttttac
      721 gaatattcag attatcataa aaaatgcaac agacaggaat gcaataattt tgcattatct
      781 cgttgcttgt aatgcaaata tgcattattg tcagtgtgtg acaataacag agagatgacg
      841 atggcgacca ttaacgcacg tattgatgat gatataaaga accaagcaga tgaagtgctg
      901 aagctgatga atatcagtca aactcaggct attgcagctt tttatcagta tattactgaa
      961 caaaagaaac tgccatttgt gatcacctca attgtaaaaa cgccgcatga tttattacgt
     1021 gaaagtacag acatgttagc tgaagctctt gcagtgattt ccaatctaca ggtgtggact
     1081 gaacagcagg atgggattgg aaaagcaaag ctaatggagt attaccgccg cctcgacgca
     1141 ctgtactgtt gtgcaaaaga gaaaataggt cttctttcag ataaccggga tgctgaacta
     1201 ggctgtgtcc cttaatcatc tgagttatag taactgtctg tttacacaac atattgaact
     1261 atggctcgct acgaccttcc cgatgaggca tggacca
//
LOCUS       DAZSNM010000429         1285 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZSNM010000429 DAZSNM010000000
VERSION     DAZSNM010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.436"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..570)
                     /locus_tag="ACHW42_004411"
                     /pseudo
     CDS             complement(1..570)
                     /locus_tag="ACHW42_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            578..1004
                     /gene="tnpA"
                     /locus_tag="ACHW42_004412"
                     /pseudo
     CDS             578..1004
                     /gene="tnpA"
                     /locus_tag="ACHW42_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          342 a          321 c          283 g          339 t
ORIGIN      
        1 ggcaagttta gccacgccag catccagccc gaccattgat gctgaagggt gaaccggagt
       61 tgatacttca ctttctgcct gaatactgat gtaccactta ccgcaggact ggctgacagt
      121 gacatttttc acaacacccg tgacctgacg gctgttccgg tagcgcatcc agccaagttt
      181 tggcagaaaa atacggctgt tttcctgagc gagcttaacg ccctgcggat agcggaatgc
      241 atcattctgt caccgctttt tgaatcaggg gaacgccgag cgcttctgga aaaagttttt
      301 gcaggcctgc tcagggtctt tcagtgactg ttgcaatggc tgtgacggag catctttaat
      361 ccattgcgtt tcagtggcat ttttccactc aaccaatcag gaagccattt tcgcgtaagg
      421 gatgtctttg ttaccggcct tatgattctc attctgacgt gccagagtac gactgaaaac
      481 gaaacgacat gcgcctgcga agcgcctcat ttcacgcgcc tgttgaccac cgggtcttaa
      541 ccggaatttg aatacctgta gtcgtttcac agggcaaatt atactttggt ctgtgaaaaa
      601 aactgatatc cgacgcggaa ggcactgtgt tttcctgatg catgctcacc gggtctttgt
      661 taccaagtac cgacgccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt
      721 ttcaaatgta tgtgcttatt ttgaagctga actggttgaa atggatggcg aaccagatca
      781 cgtccatctg ttaattaact atccccccaa actggcgata tccagtctgg taaacaaaca
      841 gcctcaaagg cgtatcgggt aggttactgc gacgggatcg accagatatt gctgccaggt
      901 attaccgcaa agatgttttg tggcgtcccg gctattttgc cagtagctgc ggaggtgcgc
      961 caatatccgt catccgccaa tacattgaac aacagcaaac acctggtcag atgaaaaaac
     1021 gagccttata tccccgccct gaaggacggg cttttacggt gcactggata agcctggaga
     1081 ttatctacac ctgcatggac atcattgtac tatgccccgt acatgctcca tcacatcctc
     1141 aacatcaaag tattagagcc ttgcaaacca ttatgcgtct cagtttgtct catctatctt
     1201 acgcaaaaaa aatagaacta atatcaagcg gcgctatatt gcgtattaaa taaaaccacc
     1261 tcaattacat taataaatct aaata
//
LOCUS       DAZSNM010000430         1280 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZSNM010000430 DAZSNM010000000
VERSION     DAZSNM010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.437"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..630
                     /locus_tag="ACHW42_004413"
     CDS             <1..630
                     /locus_tag="ACHW42_004413"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HFR8945930.1"
                     /translation="SIGVVNAIVGQRRYTVTLNGESNHAGTTPMGYRRDTVYAFSRIC
                     HQSIEKAKKMGDPLVLTFGKVEPRPNTVNVVPGKTTFTIDCRHTDAAVLRDFTQQLEN
                     DMRAICDEMDIGIDIDLWMDEEPVPMNKELVATLTELCEREKLNYRVMHSGAGHDAQI
                     FAPRVPTCMIFIPSINGISHNPAERTNITDLAEGVKTLALMLYQLAWQE"
     gene            641..>1280
                     /gene="allE"
                     /locus_tag="ACHW42_004414"
     CDS             641..>1280
                     /gene="allE"
                     /locus_tag="ACHW42_004414"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFR8945931.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT          336 a          302 c          322 g          320 t
ORIGIN      
        1 tcaattggcg tggtgaatgc aattgtcggg cagcgtcgct atacggtgac gctgaacggc
       61 gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc gtgatacggt ttacgctttc
      121 agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga tgggcgatcc gctggttctg
      181 acctttggca aagtagagcc gcgcccgaat acggtaaatg tggtgccggg taaaaccacg
      241 ttcaccattg attgtcgtca taccgacgct gccgtgctgc gcgatttcac ccaacagtta
      301 gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg gtattgatat cgatttatgg
      361 atggacgaag aacccgtgcc gatgaataag gagctggtcg ccaccctgac agaattgtgt
      421 gaaagagaaa aactgaatta ccgggtgatg cacagtggtg ccgggcacga cgcgcaaatt
      481 ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca gcatcaacgg gatcagccat
      541 aacccggcgg aacgcaccaa tattactgac cttgccgaag gggtcaaaac gttggcactc
      601 atgctttatc aacttgcctg gcaggaataa ggagtcataa atgggatatt taaataacgt
      661 caccggttac cgcgaagatt tactggctaa ccgtgcgatt gttaaacacg gtaatttcgc
      721 actgttaacc ccggatggtc tgataaaaaa tattattccg gactttgaaa attgtgacgc
      781 gacaatcctc tccacgccaa agctgggtgc ctcttttgtt gattatctgg tcacactgca
      841 tcaaaacggt ggcaaccaac agggcttcgg tggcgaaggc attgaaacgt tcctgtatgt
      901 gatctctgga aatatcactg ccaaagccga aggcaaaaca tttgccttaa gcgaaggtgg
      961 ctatctttat tgcccaccag gctccttaat gacgtttgtt aacgcccagg ccgaagacag
     1021 ccaaatcttt ttatataagc gccgctatgt tccggtagaa ggccatgcac cgtggctgtt
     1081 ttctggcaat gccagcgaac tggaacgcat tcactatgaa ggtatggatg atgtgatcct
     1141 gctggatttt ctgcccaaag agttaggttt tgatatgaac atgcatatcc tctcttttgc
     1201 accaggtgcc agccacggtt atatcgaaac acacgttcag gaacatggtg cctatattct
     1261 ttccggtcag ggggtttata
//
LOCUS       DAZSNM010000431         1277 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZSNM010000431 DAZSNM010000000
VERSION     DAZSNM010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.438"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="ACHW42_004415"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="ACHW42_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFR8945932.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          358 a          229 c          286 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DAZSNM010000432         1275 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZSNM010000432 DAZSNM010000000
VERSION     DAZSNM010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.439"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            34..435
                     /gene="gpFI"
                     /locus_tag="ACHW42_004416"
     CDS             34..435
                     /gene="gpFI"
                     /locus_tag="ACHW42_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR8945933.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="ACHW42_004417"
     CDS             447..818
                     /locus_tag="ACHW42_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR8945934.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1275
                     /locus_tag="ACHW42_004418"
     CDS             805..>1275
                     /locus_tag="ACHW42_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8945935.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          286 c          396 g          290 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atccgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccg
//
LOCUS       DAZSNM010000433         1265 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZSNM010000433 DAZSNM010000000
VERSION     DAZSNM010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.440"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(428..793)
                     /locus_tag="ACHW42_004419"
     CDS             complement(428..793)
                     /locus_tag="ACHW42_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR8945936.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            complement(793..1245)
                     /locus_tag="ACHW42_004420"
     CDS             complement(793..1245)
                     /locus_tag="ACHW42_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945937.1"
                     /translation="MHELYAAPRMRQPDGWKAVAVAWKVTFTQVDQESNTFTAIYFYK
                     AEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALSDSSLGSDEIEEIK
                     TGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLKGDK"
BASE COUNT          316 a          339 c          278 g          332 t
ORIGIN      
        1 gtaagaggct aacctacatt tgtgagacat agattgggcc tcagattaat gttaagcgtc
       61 ttgcaggacg cgtaatgtta actggggctt ttctctgtct gccttacagt ggcatgcccg
      121 aggcagacag cctcaagcac ccgcagcaat cttaccgata ccgataagaa aacgctattt
      181 tttattgcca gcaccttcta tccaggctaa tttatctgcg tcagaacggc gcaatgcgct
      241 cgcctgagat acgtttactt gccatggtaa caacaggtaa cggcaattgc tgtttctgtt
      301 ttgtttcctt caaaaacccc ggaccatcaa tccggggttt ttgtttgtta accccaacgg
      361 caaatcgaat acaccaccag cgccaccgcc atcgcaattc ctaccgtggt gaatgcctca
      421 ggccaggtca ttgattcacc tcctgcggtg gttccggtag cggcatccag tgagttgagc
      481 tctctacttc aacgccgtct ttgtccacaa aagccatctt attcccgcca cgagtggggc
      541 aaaaaacctt atcccaggaa ccggggaata cattcccgag atcatcgaga agaatcacat
      601 cgcaatattc agcaggaact gcatcactac agcttatcca gccagccaga gttaccggag
      661 agttgccagc cttacgcatg gccatttcca cgatttcaac catatctcct ggtggaattt
      721 tacaaagttg cccaatacat ttctgctgcc tggcatattc gagaatgtgc tccagtttgg
      781 ttcgattaat cattatttat ctcccttaag catggcagcg cggcaggcgt tccagccatc
      841 aacataatca aacgtattac tatcgtttgg cccgatttca tccggcacta tcggctctgg
      901 ttggatagtg acgttggcaa aggcagcacg caaaccggtt ttaatttctt cgatttcatc
      961 agagccaagc gatgaatctg acagtgcgtg atggaatgcg taagccatgt cgtcgtttac
     1021 tgcaaccggc actgccggcg ctgcgtaaag cggtgttata tctgcccgaa aatcacatgc
     1081 tttatgcagt cgtacccaac gctcaacttc cgctttgtaa aaatatatag cagtgaacgt
     1141 attagattcc tggtcaactt gagtaaacgt caccttccac gctacagcta cggctttcca
     1201 tccatcaggt tgtcgcattc gcggggcagc ataaagctca tgcattccat caggcagcga
     1261 gtgac
//
LOCUS       DAZSNM010000434         1261 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZSNM010000434 DAZSNM010000000
VERSION     DAZSNM010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.441"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(41..100)
                     /locus_tag="ACHW42_004421"
     CDS             complement(41..100)
                     /locus_tag="ACHW42_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR8945938.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..>1261)
                     /locus_tag="ACHW42_004422"
     CDS             complement(339..>1261)
                     /locus_tag="ACHW42_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flotillin domain-containing protein"
                     /protein_id="HFR8945939.1"
                     /translation="QEAFMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAER
                     QIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEA
                     RANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAA
                     EMQAAAIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEK
                     SVEPMKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDS
                     LLNEIGVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          249 a          340 c          307 g          365 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 t
//
LOCUS       DAZSNM010000435         1236 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZSNM010000435 DAZSNM010000000
VERSION     DAZSNM010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.442"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            191..>1236
                     /locus_tag="ACHW42_004423"
     CDS             191..>1236
                     /locus_tag="ACHW42_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8945940.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          401 a          282 c          196 g          357 t
ORIGIN      
        1 atcctctgca ttgtgaatat gtaaaacatc aaaaaaagat gaaccaaata tacgaaaaaa
       61 gcaaatatac ttactagggt agaaagcatg atataacact cattccctta tggcgaatat
      121 tctgaaacac cctcctcatt aggagagata cattaaatac aatttattca caatctaatt
      181 ccatatccat atgcgagaaa ttaatatgct cagaaacatt tcatcctgtt tatttccaca
      241 tatcagcaca attacatccc ccaaccatta tttgtccgaa tgggatgatt gggagaaaca
      301 ggggttaccg gaagaacagc gtactgaggc ggtaagaaga cttcgtgcat gtcttacctc
      361 taaggggcat aaactggacc tgcgagcctt ggcgctttcc tcgctacctg tactccctgc
      421 ttgcattaaa aagcttgatg tgagctgtaa taaattaacc atccttactg atttacctga
      481 aaatattaaa gaacttattg caagagataa tttcttaaca catatatctg cattaccaca
      541 ttatctaata actttggatg tgtccgaaaa tcaattagag aatctgccgt tattaccaga
      601 caccatcaaa tcactaagcg cagagtataa taggttatcc acactgcctt cattaccctt
      661 gaatttaaaa aaacttgagg ttaggaacaa cgaactgcaa actcttccat ctctgccttc
      721 taatcttaag atacttaagg ttgcgcacaa ccatcttact gaactgcccc ctttacctag
      781 gagactgcaa cttctttttg catatagcaa tagattaagc aacttaccaa acatccaaga
      841 aaatattatc atgagaagat ttttttattt tgaaaacaac caaataacta caatcccgac
      901 aaatcttttt cgtttagatc ctcatataac tattgagatt gcaaataacc ccttatcaga
      961 tcaaactctg ctattcttaa tacagcaaac ttcggttcca aattttaacg ggcctcagtt
     1021 tcgtatttcc ctgtcagacc aaaacagact gtttttacgc cagatgttgc cgcaaaattt
     1081 acattcgcgc catatcagag tcatcactga aggggggcag aactttcaga tcccccctct
     1141 tcccgaaact gtggcagcct ggtttcctga agcagatcgt cgggaggttt ctacacaatg
     1201 gacttctttt tccaccgagg agaattcccg ggcatt
//
LOCUS       DAZSNM010000436         1232 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZSNM010000436 DAZSNM010000000
VERSION     DAZSNM010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.443"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACHW42_004424"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACHW42_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR8945941.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          365 a          206 c          294 g          367 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gt
//
LOCUS       DAZSNM010000437         1228 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZSNM010000437 DAZSNM010000000
VERSION     DAZSNM010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.444"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..368
                     /gene="yebU"
                     /locus_tag="ACHW42_004425"
                     /pseudo
     CDS             1..368
                     /gene="yebU"
                     /locus_tag="ACHW42_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="in Escherichia coli this enzyme specifically
                     methylates C1407 of the 16S rRNA; internal stop;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            486..722
                     /locus_tag="ACHW42_004426"
     CDS             486..722
                     /locus_tag="ACHW42_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFR8945942.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            827..1018
                     /locus_tag="ACHW42_004427"
     CDS             827..1018
                     /locus_tag="ACHW42_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR8945943.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(1043..>1228)
                     /locus_tag="ACHW42_004428"
     CDS             complement(1043..>1228)
                     /locus_tag="ACHW42_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR8945944.1"
                     /translation="YEWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHR
                     VDIGNLHYIDTGAVFGG"
BASE COUNT          314 a          299 c          305 g          310 t
ORIGIN      
        1 ggcaacgcga caaagaactg tggttgttcc cggtaggcat tgaagccctg atcggtaaag
       61 tccgattttc tcgcttaggg attaaacttg ccgaaacgca taacaaaggt tatcgctggc
      121 agcatgaagc ggttattgcc catgcctccc ccgacaatgt aaacgctttt gaactgacac
      181 cgcaggaagc gtaggagtgg tatcgcgggc gcgatgttta cccgcaagcc gcgccagtgg
      241 cggatgacgt gttggttact ttccagcatc agccgattgg tttagccaaa cggattggtt
      301 cgcgactgaa aaacagctac ccgcgtgaac tggtgcggga cgggaaactt tttaccagta
      361 acgcatgaca gcgcacaaaa aaagcgcact ttttgactgg cacattcgcc ggcctcaact
      421 aggctgaaaa attgtgcgat cggactggtc gtactacaat cagcagctaa atggagagca
      481 cgaagatgaa aaccagtgtg cgcataggcg cttttgaaat cgacgacggc gaattacacg
      541 gtgaatcgcc gggtgatcga acgttaacca ttccttgtaa atctgacccc gatttatgta
      601 tgcaactgga tgcctgggat gctgaaacca gtatccctgc cctgctaaat ggcgaacact
      661 ctgtccttta ccgtacccgt tacgatcaac aatctgatgc ctggattatg cgtcttgcct
      721 gatccaaaaa gaacccgtcg gcatggcggg ttatttgtcc tggttattcc cccgttgtaa
      781 aatctctcct aaacttaacg gtacggcacc acacttcggg gatgaaatgt tcgcgctggt
      841 actttttgtt tgctacctgg atggcggttg tgaagatatt gttgtggatg tctacaacac
      901 ggaacagcag tgtctttatt ctatgagcga tcaacggatc cgccatggca gttgttttcc
      961 gattgaggat tttatagatg gtttctggca accagcacag gagtatggtg atttttaatt
     1021 attgcagttg cacaagagtc agttagcccc caaagacagc accggtatca atataatgca
     1081 ggttgccaat atccacgcga tgtcgcaacg gtgtatgacc aaaccagaaa tgatcagcac
     1141 ctgtaattcc ctgccctttt gggcgttcac ctaatcgcga gcggctccac aagacctgat
     1201 gcaaatcaac gtccttttgc cattcata
//
LOCUS       DAZSNM010000438         1227 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZSNM010000438 DAZSNM010000000
VERSION     DAZSNM010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.445"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1122
                     /locus_tag="ACHW42_004429"
     CDS             <1..1122
                     /locus_tag="ACHW42_004429"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR8945945.1"
                     /translation="RRVLLIDNGKILGRKILMSGGGRCNFTNLYVEPGAYLSQNPHFC
                     KSALARFTQWDFIDLVNKHGIAWHEKTLGQLFCDDSAQQIVDMLVDECEKGNVTFRLR
                     SEVLSVAKDETGFTLDLNGMTVGCEKLVIATGGLSMPGLGASPFGYKIAEQFGLNVLP
                     TRAGLVPFTLHKPLLEELQVLAGVAVPSVITAENGTVFRENLLFTHRGLSGPAVLQIS
                     SYWQPGEFVSINLLPDVDLETFLNEQRNAHPNQSLKNTLAVHLPKRLVERLQQLGQIP
                     DVSLKQLNVCDQQALISTLTDWRVQPNGTEGYRTAEVTLGGVDTNELSSRTMEARKVP
                     GLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQDLASIVK"
BASE COUNT          274 a          303 c          347 g          303 t
ORIGIN      
        1 cgccgggttc tgctgatcga taatggtaaa atactagggc gcaaaatcct tatgtctggc
       61 ggtgggcgct gcaactttac caacctttat gtcgaaccag gcgcttatct gagccagaat
      121 ccgcattttt gtaagtctgc actcgcacgt tttacccagt gggatttcat tgatctggtc
      181 aataaacacg gtatcgcctg gcacgagaaa acgttagggc aactcttctg cgatgactcc
      241 gcgcagcaga ttgtcgacat gctggtggat gagtgcgaga agggcaatgt aaccttcaga
      301 ttgcgtagcg aagtgctgag tgtggcgaag gatgaaacag gcttcacgct tgatctgaac
      361 ggcatgactg tcggttgcga aaagctggtc atcgcgaccg gtgggctgtc aatgccgggg
      421 ctgggcgcat cgccgtttgg ttataagatt gccgaacaat ttggcctcaa cgtgctgccg
      481 acccgcgcgg gtctggtgcc attcactctg cataaaccgt tgctcgaaga gttacaggtg
      541 ctggcgggcg tggcggtgcc ttccgtgatt accgctgaaa acggcaccgt tttccgtgag
      601 aacttactct tcacccaccg cggcttatct ggaccggcgg tgttgcagat ttcaagctac
      661 tggcaaccgg gtgaatttgt cagtatcaat ctgctaccgg atgtggacct cgaaaccttc
      721 ctgaatgagc agcgtaacgc acatccgaat caaagcctga aaaacacact ggcggttcat
      781 ctaccgaagc ggttggttga acgcttacag caactcgggc aaatcccgga tgtttcgcta
      841 aaacagctca acgtgtgtga ccaacaggca ctgattagca cattgaccga ctggcgcgta
      901 caacccaacg gcactgaagg ctatcgcact gccgaagtga cgctcggcgg cgtggacacc
      961 aacgaacttt cttcacggac gatggaagcg cgcaaagtgc ctgggctgta cttcatcggt
     1021 gaagtgatgg acgttaccgg ctggctgggg ggctataact tccagtgggc gtggtcgagt
     1081 gcatgggctt gtgcgcagga tttggcgtca attgtgaaat aaccatgacg ttttcccgtc
     1141 agccgtttgt atatgtcgtt attaagtcat tttggtacgg ttctgcatcc gacattctgg
     1201 tgttaactcg ctgttttgtt agcgaat
//
LOCUS       DAZSNM010000439         1218 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZSNM010000439 DAZSNM010000000
VERSION     DAZSNM010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.446"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1103)
                     /locus_tag="ACHW42_004430"
     CDS             complement(<1..1103)
                     /locus_tag="ACHW42_004430"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR8945946.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICT"
BASE COUNT          302 a          313 c          312 g          291 t
ORIGIN      
        1 gtacagatga actcatacgg cccctgtttg gcgatttcat ccgccagata agcggcaacc
       61 tggtaagagg cgttctgtac tttggtatag ccttcacgac cgaggcgcag gaattcatag
      121 tactgtgcaa ttacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatttga
      181 ccacccaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccaggaag
      361 ccaccgctgg cagcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta
      421 tccagcgcat cgtgcagcgg ttgtgggaac tcatagttac cggtgtaggt cacgccgaaa
      481 gtcggcacca cgccgatggt gttttcgtca caggcttcaa tcatgcgttt cgggtccata
      541 aacaactgac cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat
      601 ttatgccagc agatttgtac cggaccgcac actaggtttg gtttatccgt tggtttgcct
      661 gcagcttcca tacgcttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc
      721 tcggaagaac caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc
      781 cacagatcgg caaccatatt tacgcaacgc aggtcgatgg ctgcggattg cggatattct
      841 tctttgtcga tccagttttt attgatcgac aaatccatca atttatggac gttttcgtcg
      901 tcccaggtct ggcagaaagt ggctaggttc tgacgagcgt tgccatcaag atataattca
      961 tcattgataa tctggaatgc gacatcatcg cgcatttcgt gcagcggaaa tcgttttgac
     1021 tccgcgatag tagaaatggc ctttgcgcca aaacgtgaat cgagtagttc tgagcggaaa
     1081 tccgttaaca gcttctggtc catttcgaac tccttaaatt tatttgaagg caataaaaaa
     1141 gtaggattta tccgcaatgg aagcaaggca ttacaggctt aatttaaata acaaaatcct
     1201 aagcagaaaa acgacttt
//
LOCUS       DAZSNM010000440         1194 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZSNM010000440 DAZSNM010000000
VERSION     DAZSNM010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.447"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..496
                     /locus_tag="ACHW42_004431"
     CDS             <1..496
                     /locus_tag="ACHW42_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR8945947.1"
                     /translation="LHASTNAPGSDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQK
                     THYSDTRWHEDSVRNRWFSVMAGLSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDN
                     KGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGV
                     GYKF"
     gene            560..>1194
                     /locus_tag="ACHW42_004432"
     CDS             560..>1194
                     /locus_tag="ACHW42_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR8945948.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT          279 a          285 c          387 g          243 t
ORIGIN      
        1 tcttcatgcc tcgacgaacg ctcccggcag tgatgatctg aacgggatta acgtgaaata
       61 ccgttatgag tttacggaca cgctggggct ggtgacgtca ttcagctatg ccaacgctga
      121 agatgagcaa aaaacgcatt acagtgatac ccgctggcat gaggattccg tgcgtaaccg
      181 ctggttcagc gtgatggcgg ggctgtctgt gcgcgtgaat gaatggttca gcgcgtatgc
      241 gatggcgggc gtggcttaca gccgtgtgtc gaccttctcc ggggattatc tccgcgtaac
      301 tgacaacaag gggaaaacgc acgatgtgct gaccgggagt gatgacaacc gccacagcaa
      361 cacgtctctg gcgtgggggg ctggcgtgca gtttaacccg accgaatccg tggccattga
      421 ccttgcttat gaaggttccg gcagtggcga ctggcgcact gacggtttca tcgtgggtgt
      481 cggttataag ttctgattag ccaggtaaca cagtgttatg acagcccgct ggttcaggcg
      541 ggctttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag atggggcggg
      601 taaacctgtc gtaaattgtg cgattgaact gcgggccaga agaaccagtc cgaccgttgt
      661 ggcacacgtt gttgccactt gcgtgacgga caataacggt gcttatgtga ttgaggctga
      721 gccggggtat tacgaggttg cgcttcactg taacggctgg cagccaaccc gtgtcgggga
      781 tattgatgtg gcaccgactg atgcaccggg gacactgaac gcgtttctga atgcaccaaa
      841 ggatggtgat ttacgtccgg aggtgatgaa gcgctttgag gaaatggtgg cgcaggcgca
      901 gcagagtgcc ggggctgcag ccggaaacgc acagcagacg gcgcaggatg tggcggcagc
      961 cgcaacggcc cgtgatgatg cacaacgttt tgcggagaaa gcccgacagg atgcaaccgt
     1021 cacagctgag gacagaaagg ccactgcgga agatgtgaca agcacaggag caaatgcagc
     1081 cgcagccgga cagagcgcac aggatgccgc aggttatgcc cgcgcagcag aacaggccaa
     1141 aaatgacatt gatgctgcgc tgaccggcac tctgaaaatg gctaaccatc tgtc
//
LOCUS       DAZSNM010000441         1192 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZSNM010000441 DAZSNM010000000
VERSION     DAZSNM010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.448"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            85..243
                     /locus_tag="ACHW42_004433"
     CDS             85..243
                     /locus_tag="ACHW42_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR8945949.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            327..776
                     /gene="kbp"
                     /locus_tag="ACHW42_004434"
     CDS             327..776
                     /gene="kbp"
                     /locus_tag="ACHW42_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR8945950.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(777..>1192)
                     /gene="csiR"
                     /locus_tag="ACHW42_004435"
     CDS             complement(777..>1192)
                     /gene="csiR"
                     /locus_tag="ACHW42_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR8945951.1"
                     /translation="MEAMLVSLAIARGGDEWEADVLAKAHLLSKLEACDASEKMLDEW
                     DLRHQAFHTAIVAGCGSHYLLQMRERLFDLAARYRFIWLRRTVLSVEMLEDKHDQHQT
                     LTAAVLPRDTARASELMRQHLLTPIPIIQQAMAGN"
BASE COUNT          303 a          301 c          298 g          290 t
ORIGIN      
        1 actggcaggc tccgctaaaa ttactacgct taagagataa aatctctttt taaacaatga
       61 gtaattttct tatagggagt acatatgggt ttctggagaa tcgtcatcac catcattctg
      121 ccgccgatcg gcgtgctgct cggtaaaggg ttcggttggg cgttcattat taatattctg
      181 ctgacactgc ttgggtatat ccccggtttg attcacgcgt tctgggtgca aacccgcgat
      241 taaacctgtg ctcacccccg cggcgttaac gcgccgcgtt cctctgctac actttctgag
      301 tgtttccgtt aacgaatgag gtagctatgg gtctgttcaa ttttgttaaa gatgccggag
      361 aaaaactctg ggatgcggtt acaggtcagc acgataaaga cgatcaggcg aagaaggtgc
      421 aggagcatct gagcaaaacc ggtatacctg atgccgataa agtgaacatt caaattgccg
      481 acggcaaagc gacggtcact ggtgacggcc tgagtcagga ggcgaaggag aaaatccttg
      541 ttgcggtggg gaatatttcc ggtattgcca gtgttgatga tcaggtgaaa acggcgacac
      601 cagccactgc cagccagttt tataccgtta agtctggcga cactctgagt gccatttcca
      661 aacaggtcta cggtaacgct aatctgtaca ataaaatctt cgaagcgaat aaaccgatgc
      721 taaaaagccc ggataaaatt tatccggggc aagtgttgcg tattccggaa gagtagttaa
      781 ttgccagcca tcgcctgctg gataatggga attggcgtca gtaaatgctg gcgcattaac
      841 tcactggcgc gcgctgtatc tcgcggcagt accgccgcag tcagggtctg gtgctgatcg
      901 tgtttatcct ccagcatttc caccgaaagc accgttcgcc gcagccagat aaatcgataa
      961 cgcgccgcca gatcaaacaa ccgttcacgc atttgcagca aatagtgaga accacagccc
     1021 gccacaattg ctgtatgaaa cgcctgatga cgcagatccc actcatcaag cattttctcg
     1081 ctggcgtcac aggcttccag cttactgagc agatgcgctt ttgcgagaac gtctgcctcc
     1141 cattcatcgc caccgcgggc aatcgccaga ctgaccagca tcgcttccat at
//
LOCUS       DAZSNM010000442         1175 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZSNM010000442 DAZSNM010000000
VERSION     DAZSNM010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.449"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>1175
                     /locus_tag="ACHW42_004436"
     CDS             <1..>1175
                     /locus_tag="ACHW42_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR8945952.1"
                     /translation="PETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLDSSWSL
                     FRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVSD
                     SPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDE
                     TRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESK
                     NLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLLVSRAS
                     YEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETSDRFTVVQGYA
                     GVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQL
                     "
BASE COUNT          249 a          313 c          382 g          231 t
ORIGIN      
        1 ccggagacac gcagtcatga ccgctatgtg acagaacgtg tgacggcgca gagtcacagc
       61 ctgaccctgc ggaatgcgca gggcgaaacg caggtggtgc gtatttcttc cctggacagc
      121 agctggtcgc tgttccggcc ggaaaaaatg ccggtggcag acggtgagcg tctgagggtg
      181 acagggaaaa ttcccggact ccgcgtctcc ggcggtgacc gcctgcaggt ggcatccgtc
      241 agtgaagatg cgatgacggt tgttgtgccg ggacgggctg aaccggccac cctgcctgtg
      301 agcgattcac cgttcactgc actgaagctg gagaacggct gggtggaaac gcccgggcat
      361 tccgtcagcg acagtgcgac ggtttttgcc tccgtcacac agatggcaat ggacaatgcc
      421 accctgaatg gtctggcccg cagtggtcgt gatgtccggc tgtattcctc actggatgaa
      481 acccgtactg cggaaaaact tgcccgccat ccctccttta cggtggtttc tgagcagata
      541 aaggcgcgtg ccggtgagac attgctggaa accgctatca gtctgcagaa agccgggctt
      601 cacacgccgg cacagcaggc cattcatctg gcccttcctg tgctggaaag taaaaacctg
      661 gccttcagca tggtggacct gctgacagag gcgaagtcgt ttgctgcaga aggaaccggt
      721 tttgctgacc tgggagggga aatcaatgcg cagataaaac ggggtgattt actgtatgtg
      781 gatgtggcaa aaggctatgg cacaggcctg ctggtttccc gtgcgtcgta tgaggcagaa
      841 aagagcattc ttcgccatat tctcgaaggt aaggaggcgg tcacgccgct gatggagaga
      901 gtacctggcg aactcatgga gaaactgaca tcaggacagc gcgccgccac ccgtatgata
      961 ctggaaacgt ctgaccgttt cacggtggtg cagggctatg ccggtgtggg taagaccaca
     1021 cagttccggg cggtgatgtc agccgtgaac atgctgccgg agagtgaacg tccccgtgtc
     1081 gtggggctgg ggcccacaca ccgtgcggtc ggggagatgc gcagcgccgg cgtggatgca
     1141 cagacactgg cgtcctttct gcatgacacg cagct
//
LOCUS       DAZSNM010000443         1169 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZSNM010000443 DAZSNM010000000
VERSION     DAZSNM010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.450"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..268
                     /locus_tag="ACHW42_004437"
     CDS             <1..268
                     /locus_tag="ACHW42_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR8945953.1"
                     /translation="DALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASR
                     RQINQLLNWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(322..>1169)
                     /gene="yeaX"
                     /locus_tag="ACHW42_004438"
     CDS             complement(322..>1169)
                     /gene="yeaX"
                     /locus_tag="ACHW42_004438"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR8945954.1"
                     /translation="SHILVQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGS
                     RFLHQQVKVGDRLTISTPNNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQ
                     LHYCSRNPESCAFRAELVQHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPE
                     ALIEAVRSEAARLDIAADTLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVI
                     ENNKAAKVECLCREGVCGTCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKR
                     LVLDL"
BASE COUNT          293 a          306 c          281 g          289 t
ORIGIN      
        1 agatgcctta ccgcagccga tgcttaacct gatggacatg cctggttatc gtaaagcgtt
       61 taaagcgatt aagtcgctga ttactgacgt gagcgaaacg cataagatca gcgccgaatt
      121 gctggcatcg cgtcggcaaa tcaaccaact gctgaactgg cactggaaac tgaaaccgca
      181 gaacaatttg ccggagctga tttccggctg gcgtggtgag ctgatggcgg aagcattaca
      241 caatttattg caggaatatc cgcagtaaaa tcttccgaag ccggactggg cgcgctcagc
      301 gccacatccg gctttggcaa actacaaatc caacaccagg cgtttaccct tcgcacgcga
      361 acaacagatc aacatacttt gctggctggc acgctcttca tcgctaaaat actgatcccg
      421 atggtcagct tcaccttcca gtattgccgt ttcgcaggtt ccgcataccc cttcacgaca
      481 taaacattcc actttcgcgg ctttattatt ttcaataacc tgcaaaatag tcatctcttc
      541 cggcaccaca aactcttttc cggaacgggc aagcaccagg gtaaatgcat cgccggtttt
      601 gtcttcgata gcaaattgct caaagtgcag tgtatcggcg gcgatgtcca gacgcgcagc
      661 ttcacttctt accgcttcaa ttagcgcctc ggggccacag gtataaacgt gtgtgccagg
      721 ttcgatatcc gccaataatc gcgctaattc cagtcgtgtt ccggttgatg aatgatgcaa
      781 atggactttc tcagcctgcg gatgctggac tagctcagca cgaaatgcgc aactttctgg
      841 atttcgcgag cagtaatgta gctgccagtc gatgtcgctg tgttgcaact ctgccatgtg
      901 cgacaggaaa ggggtgatac caataccgcc cacgataaac agatgctttc tggctgaggg
      961 aattagcgca aacaggttat taggcgttga gatcgttaac cgatcgccca cttttacctg
     1021 ctgatgcaaa aagcgggaac cgccgcgcga gttttcttcc agtcgaacgg caatctgata
     1081 actagaggtg ttatgcggtg aactcagtag tgaatacgca ttgctgtact ggttatcacc
     1141 atcgctcatc tgcactagga tgtgacttc
//
LOCUS       DAZSNM010000444         1165 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZSNM010000444 DAZSNM010000000
VERSION     DAZSNM010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.451"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            7..657
                     /locus_tag="ACHW42_004439"
     CDS             7..657
                     /locus_tag="ACHW42_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945955.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="ACHW42_004440"
     CDS             629..1063
                     /locus_tag="ACHW42_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR8945956.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
BASE COUNT          328 a          236 c          292 g          309 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta tttta
//
LOCUS       DAZSNM010000445         1160 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZSNM010000445 DAZSNM010000000
VERSION     DAZSNM010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.452"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(327..572)
                     /locus_tag="ACHW42_004441"
     CDS             complement(327..572)
                     /locus_tag="ACHW42_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945957.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
BASE COUNT          296 a          248 c          274 g          342 t
ORIGIN      
        1 ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta acaaattcaa
       61 tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta atgctctaac
      121 ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct tttaatctca
      181 ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt ttgatgttga
      241 tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac tgttgcgata
      301 taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac cagaccctga
      361 ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt gtgtctgcct
      421 ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag ctgcacgttg
      481 agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca gtgcatccgc
      541 tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt gactgtcgtg
      601 ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta aattgctttc
      661 caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata tgcaatgggc
      721 tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa cgttctatct
      781 ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc ctatggatca
      841 tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat cagagtgaaa
      901 aatgttcggt ttgatgttgg gggggactga ggaggggcca acgcaggtgg tatgcgtttt
      961 acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt agccaggtgc
     1021 tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc acagtgacca
     1081 gaagggggct acttagaaga aatgttcagg ctgggtggaa gaggtaaagc agtgacatca
     1141 gcattcaaag tgaccggata
//
LOCUS       DAZSNM010000446         1160 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZSNM010000446 DAZSNM010000000
VERSION     DAZSNM010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.453"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            116..>1160
                     /gene="cadB"
                     /locus_tag="ACHW42_004442"
     CDS             116..>1160
                     /gene="cadB"
                     /locus_tag="ACHW42_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR8945958.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          246 a          256 c          298 g          360 t
ORIGIN      
        1 ctttaggatt aatccttttt tcgtgagtaa tcttatcgcc agtttggtct ggtcaggaaa
       61 tagttataca tcatgacccg gactccaaat tcaaaaatga aattaggaga agagcatgag
      121 ttctgccaag aagatcgggc tatttgcctg taccggtgtt gttgccggta atatgatggg
      181 gagcggtatt gcattattac ctgcgaacct agcaagtatc ggtggtattg ctatctgggg
      241 ttggattatc tctattattg gtgcaatgtc gctggcgtat gtatatgccc gactggcaac
      301 aaaaaacccg caacaaggtg gcccaattgc ttatgccgga gaaatttccc ctgcatttgg
      361 ttttcagaca ggtgttcttt attaccatgc taactggatt ggtaacctgg cgattggtat
      421 taccgctgta tcttatcttt ccaccttctt cccagtatta aatgatcctg ttccggcggg
      481 tatcgcctgt attgctatcg tctgggtatt tacctttgta aatatgctcg gcggtacctg
      541 ggtaagccgt ttaaccacta ttggtctggt gctggttctt attcctgtgg tgatgactgc
      601 tattgttggc tggcattggt ttgatgcggc aacttatgca gctaactgga atactgcgga
      661 taccactgat ggtcatgcga tcattaaaag tattctgctc tgcctgtggg ccttcgtggg
      721 tgttgaatcc gcagcagtaa gtactggtat ggttaaaaac ccgaaacgta ccgttccgct
      781 ggcaaccatg ctgggtactg gtttagcagg tattgtttat atcgctgcga ctcaggtgct
      841 ttccggtatg tatccgtctt ctgtaatggc ggcttccggt gctccgtttg caatcagtgc
      901 ttcaactatc ctcggtaact gggctgcgcc gctggtttct gcattcaccg cctttgcgtg
      961 cctgacttct ctgggctcct ggatgatgtt ggtaggccag gcaggtgtac gtgccgctaa
     1021 cgacggtaac ttcccgaaag tttatggtga agtcgacagc aacggtattc cgaaaaaagg
     1081 tctgctgctg gctgcagtga aaatgactgc cctgatgatc ctcatcactc tgatgaactc
     1141 tgccggtggt aaagcatctg
//
LOCUS       DAZSNM010000447         1140 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZSNM010000447 DAZSNM010000000
VERSION     DAZSNM010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.454"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..730
                     /locus_tag="ACHW42_004443"
     CDS             <1..730
                     /locus_tag="ACHW42_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFR8945959.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGV
                     HNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRP
                     LPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(732..958)
                     /locus_tag="ACHW42_004444"
                     /pseudo
     CDS             complement(732..958)
                     /locus_tag="ACHW42_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          298 a          283 c          247 g          312 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga
      781 ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga
      841 cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg
      901 cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta
      961 cacccggatg gtaaacaggc acttccttgt tgtagaagcc gttatattgt ccataatgtc
     1021 tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc
     1081 cagccggttt gaattcatct cacaaccctg catagagcga atctcctgcc tgcacgtcac
//
LOCUS       DAZSNM010000448         1104 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZSNM010000448 DAZSNM010000000
VERSION     DAZSNM010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.455"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            95..697
                     /gene="fimB"
                     /locus_tag="ACHW42_004445"
                     /pseudo
     CDS             95..697
                     /gene="fimB"
                     /locus_tag="ACHW42_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
BASE COUNT          336 a          187 c          209 g          372 t
ORIGIN      
        1 aacgttcgct tttataaaca taatcaggat aaaaatgttg gattattgct aacccagcac
       61 agctagtgcg cgtctgtaat tataagggaa aacgatgagg aataaggctg ataacaaaaa
      121 aaggaacttc cttacccata gtgaaatcga gtcactcctt aaagcagcaa ataccgggcc
      181 tcatgcaaca cgtaattatt gtctgatttt gctttgtttt attcatggtt tccgggcgag
      241 tgaaatttgt cgattgagga tttcggatat tgatcttaaa gcaaagtgta tatatatcca
      301 tcgattaaaa aaaggctttt caacaacgca cccgctattg aacaaagaag ttcaggcttt
      361 aaaaaactgg ttgagtatcc gtacttctta tccgcatgct gagagcgagt gggtattttt
      421 atcacgtaag gggaatccgc tttctcggca acagttttac catattatct cgacttctgg
      481 tggtaatgcc gggttgtcac tggagattca tccgcacatg ttacgctatt cgtgtggttt
      541 tgctttggcg aatatgggaa tagatacgcg acttatctag gattatcttg ggcatcgcaa
      601 tattcgtcat actgtctggt ataccgccag caatgcaggg cgtttttacg gcatctggga
      661 tagagccaga ggacgacagc gtcatgctgt tttatagaga tagttgagat accagggatg
      721 gtgttaaaaa ctacattatt cttacgatat atcaaaaatg attaaccatt tattaattga
      781 tgttattgta agtgtgattc ttaattatca taataaacat taagttaacc atacccatac
      841 aaaatctaat gatttatgtt cttcaaaata aataaacaaa atcattcata agtttacaca
      901 tcacttaaat tctcctgttt ccacactttt ttctttattt tttaagtaac tggaagttaa
      961 tcctctgtaa tctattgtta tattgaatca aatcaatgaa aatagatgtt gtcacatcag
     1021 tgatatttta tttttgtatg atgtttaatg taattgactg atagccacat aacttcgtgt
     1081 gcggttatct ttttatctat tggg
//
LOCUS       DAZSNM010000449         1090 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZSNM010000449 DAZSNM010000000
VERSION     DAZSNM010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.456"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            113..807
                     /locus_tag="ACHW42_004446"
     CDS             join(113..359,359..807)
                     /locus_tag="ACHW42_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8945960.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
BASE COUNT          286 a          241 c          255 g          308 t
ORIGIN      
        1 taagttgtga ttattataat ttagttattg ttagtatgtt ttataatgct ggatgggtga
       61 tgcctctaat tagttgaatc tgatgtataa tacgggcttt tgaggttatc tcatgaccag
      121 cgttaatatt cattgtcccc gttgtcagtc agctcaggtt taccgccatg gtcagaaccc
      181 taaaggccgt gacagacttc gctgccgtga ctgccaccgt gtgtttcagt tcacttatac
      241 ttatcaagca cgtaagccgg gtatgaaaga gctgataact gaaatggcct ttaatggtgc
      301 cggggttcgc gataccgcca ggacactgaa aattggtagt aacaccgtca tccggacttt
      361 aaaaaactcg cgccaaagcg aataacttct tcgcctgttg cccatgctga tgtggcgttt
      421 atctgcgagc ttgatgagca atggagctac gttggcagta aagcccggca acattggctc
      481 gggtacgcgt acaacaccaa aacaggcggt gtactggcct acacttttgg tccccgaacc
      541 gatcaaacgt gccgggagct actggcactg cttacaccct tcaacatcgg catgctgacc
      601 agcgatgact ggggcagcta tggccgggag gtgccgaaga ataagcatct gaccggcaaa
      661 atattcaccc aacgcattga gcgtaataat ctgacgctac gcacccgcat taagcggttg
      721 ggtcgtaaaa caatctgctt ctcacgctca gttgagatcc acgaaaaagt gatcggagcg
      781 tttatcgaaa aacacatgtt ctactaattg gatgcactac ccagtatgta actgggtttg
      841 tccataaaat gcaccttact cagttggtgt ccaacttttg gggtgcagtt cagaaggacg
      901 ggattttacg gtgtaccgga taaatttaaa actgcctgat actgaatgta tgaggtttcg
      961 taatgctcaa ttcccctagt accgttttgt agatctccat acttccacta gtatttattt
     1021 gcaaagttcc tatatttgag gagtttaata aatttagctg tcccaagtga tttttaatag
     1081 agatagcctg
//
LOCUS       DAZSNM010000450         1078 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZSNM010000450 DAZSNM010000000
VERSION     DAZSNM010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.457"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..254
                     /locus_tag="ACHW42_004447"
     CDS             <1..254
                     /locus_tag="ACHW42_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HFR8945961.1"
                     /translation="KICGINIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSL
                     IHTTLIIATQKNKIEYMKKHILATLDGIRGITGGGRYNA"
     gene            247..>1078
                     /gene="rfbG"
                     /locus_tag="ACHW42_004448"
     CDS             247..>1078
                     /gene="rfbG"
                     /locus_tag="ACHW42_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HFR8945962.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     L"
BASE COUNT          390 a          140 c          201 g          347 t
ORIGIN      
        1 taaaaatctg tgggattaac ataccaattc actcgccagt acgtcgttat tatcgagtaa
       61 gaaatgcatt tcttttgctt agaaaaaatc atgtgcctct tttactctct attagggaag
      121 ttgttttttc tttaattcat acgactttaa ttatcgcaac tcaaaaaaat aaaattgaat
      181 atatgaaaaa acatattttg gctacgctgg atggaataag aggaattact gggggcggtc
      241 gttataatgc atagttctga tcaaaaaaga gtagctgtac ttatggctac gtataatggc
      301 gaatgctgga ttgaggaaca attaaaaagt attattgaac aaaaagatgt agacatctca
      361 atttttatat cagatgattt atcaactgat aatacattaa atatttgtga agaatttcaa
      421 ttatcatatc cctcaattat aaatatactt ccaagcgtca ataagtttgg gggggcaggt
      481 aaaaattttt atagattaat taaagatgtc gatcttgaaa actatgatta tatctgtttt
      541 agtgatcagg acgatatttg gtataaagat aaaataaaaa atgcaattga ctgcttggta
      601 tttaataatg cgaattgtta ttccagcaat gtaattgcat attatccatc agggcgaaaa
      661 aatttagttg ataaggctca gtcgcaaact caattcgatt atttttttga agcagcaggt
      721 ccggggtgta catatgttat aaaaaaagaa acgcttattg aatttaagaa gttcatcata
      781 aataataaaa atgctgcaca agatatctgt ctgcatgatt ggtttttata ttcatttgcg
      841 cggacgagaa attattcgtg gtatattgat agaaaaccaa caatgctgta cagacagcat
      901 gaaaacaacc aagtgggagc aaacattagt tttaaagcaa aatacaaacg gcttggtttg
      961 gtgagaaaca agtggtatcg aaaagaagta actaaaatag ctaatgcctt agctgatgat
     1021 agctttgtga ataatcaatt gggtaaaggt tatataggca atttgatctt ggctttaa
//
LOCUS       DAZSNM010000451         1071 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZSNM010000451 DAZSNM010000000
VERSION     DAZSNM010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.458"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(12..705)
                     /gene="ospZ"
                     /locus_tag="ACHW42_004449"
                     /pseudo
     CDS             complement(12..705)
                     /gene="ospZ"
                     /locus_tag="ACHW42_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            761..913
                     /locus_tag="ACHW42_004450"
                     /pseudo
     CDS             761..913
                     /locus_tag="ACHW42_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          322 a          211 c          180 g          358 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aatctctggc gactcatctc ttaacacttg
      181 cataatctct ctttttatat catatagttc tataaaccga tttgcccgta gttctccttg
      241 ggaagaatca tttctgaata caacaattat atacaaaaaa tcactatttt ctttagagaa
      301 gatcttacaa ctaatctctg ggagctcaca taatgcatgt aagtcaggat ggtacaaact
      361 atccacagaa aattgcccca taggacgttt tccagatata actgtcaacc agcttccttc
      421 cggtgtactg gcttcggctc tggaaatagg ctgaaaatct tccggacatg gagaatacat
      481 tgtcttcata taattaagtc gtctggattt ctcattaata aactcctcta tttttaattg
      541 tgccatactt ctggttatac cagagatata cttcttgtca tatatgtttg gtgattcatt
      601 aaagtaacca tgttcaactc taggataaat atttctcact atatattcag tatttgcagt
      661 atttattgga aatacgttct taatattctt gatgggacta atcattttaa tctctatact
      721 caaatataaa cattaccatg aacaatataa ttgttcctaa gtggaatgtc tccacggaga
      781 acactttatc tatcgggaaa tagtgcgggc aacagtgttt aattatatcg agtgcaatta
      841 caatcgctgg cgacggcacc gttggtgtgg cggcctcagt ccggaacaat ttgaaaacca
      901 gaacctcgct taggcctgtg tccatattac gtgggtaggc tcacagagcc tcacttacga
      961 ctgcgtccat attatgtggg taggatcaat aaagacgttt tatatgtgtc tgcttgcagc
     1021 cagaaaattt ataccctgca ggtatcataa acatgcttga taacttaatt g
//
LOCUS       DAZSNM010000452         1070 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZSNM010000452 DAZSNM010000000
VERSION     DAZSNM010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.459"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="ACHW42_004451"
     CDS             complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="ACHW42_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFR8945963.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          301 a          322 c          251 g          196 t
ORIGIN      
        1 tttaatccta tctgagtgtt tagcattgtt gcgctccttt catgtgcaat gccatataaa
       61 gccactggcg gagggagcgc gcagggggca gccagtcgcc gccgccccct gcacccccgg
      121 gctctggcga acaaaatcgc cgctgcgcgg taccttcagc ttatcccctt cggcaaccgg
      181 gtcgggcgcg aggtaacgtc ctgtaagacg cgccctcagc ccacatccat gtgggctgcc
      241 ccggccttcg gggaacgctt cagcgatttt gacgccacca atacccgtgc ggtttgtaat
      301 gtcgaagata aaacaaccag ggcagataaa atgccaatgc taattaatca cgcttttcca
      361 gcagagtccg gtaaatcaga ccaccgataa tgccgccgac aattggcacc acccagaaga
      421 accacagttg ttctaatgcc cagccgccct ggaagatagc aaccgcggtg ctgcgcgccg
      481 ggttaacaga agtgttagtc accggaatac tgattaagtg aatcagggtt aaggccagac
      541 caatagcgat cggcgcaaaa cctgccggag cgaatttgtc ggttgcaccg tggatcacta
      601 acaggaagcc cgcactcaat accagttcaa ctaccagcgc ggaaagcatg gaataaccgc
      661 ctggtgaatg ctcgccataa ccgttagaag caaaaccgct ggctgccgcg tcaaaacccg
      721 ttttaccact ggcaattaaa tacagcagcg ccgctgcaac aataccgccg acaacctggg
      781 caattacgta gccaacgact tcttttgccg gaaaacgtcc gccagcccat aaaccaatag
      841 tgaccgccgg gttaaaatga ccaccagaaa tatgaccaac agcgaaggcc atcgtcagaa
      901 cggtcaaacc aaatgccagc gccacgccgg caaaaccaat gcctaattcc gggaagcctg
      961 cggccagtac agcactacca cagccaccaa aaacaagcca gaaagtacca aaacattcag
     1021 ctgctaattt tctgaacata tccacctcaa ttaaaaattg accctgtgaa
//
LOCUS       DAZSNM010000453         1069 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZSNM010000453 DAZSNM010000000
VERSION     DAZSNM010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.460"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(227..652)
                     /locus_tag="ACHW42_004452"
     CDS             complement(227..652)
                     /locus_tag="ACHW42_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR8945964.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(649..1032)
                     /locus_tag="ACHW42_004453"
     CDS             complement(649..1032)
                     /locus_tag="ACHW42_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR8945965.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          253 a          308 c          265 g          243 t
ORIGIN      
        1 ctcgcgtatg gttgattgac accacagtgc gaattcggcg aatgcgagat cacggtacga
       61 ttccgcacca cactaccaat caataccccg ggttttattc aggtaagcag gactgcggat
      121 atccggtgtc gcgcctttgt gtcacgaacg gggtgggtgc gaaacaccag ataaaataca
      181 ggttggcaat acctctgaac gccctgagca ggtcgggcat ttcggattaa gtactcgcac
      241 ctccgcagtc ctcaaacaag tctggctggt agctgtaaac agacttcgta cataccgctc
      301 tgcaataaat ccccgtacca caggcctcac agaacttctt cccatgaaaa aactgctcac
      361 acatcacaca atgccactcc agcacgaccg gtaatggcga taaaaacatc gccatatcct
      421 caatgtaagg gtgggacttc tccggattca gcaccacaca gtccgccttc tgttgctcac
      481 tcagggcatg taaatcatgc tcaaaccacg ccccctgagc atctgtctgc agaatcaacc
      541 ggccatggca ggaaaggcag aaacactgcc ggatatttct gctgagcgca gagtcggcac
      601 tgataatgtg ctcacccggc gtgatcccca gccccgtttt tataccgttc attctgctcc
      661 cccctcctca ctgaagcgcc cggtctggca gtgtgtgcag tactgctccc cataataatc
      721 gtggtgacat tgcctgcagt gccagctggc tttacgtact acgggtaagg tatccgggac
      781 gaattgctgc agacgtttaa tcaaccgtat ttctctgcgt tccggcctga cataagggca
      841 ctcatgaccg tgctccgtca gcccttcgtc agcgtgttca aaccagggcc gttcagtgtc
      901 gtattgcggg tggtatctga gtgcactgcc gcaaaggtgg caggtgtagc tatcgtaagg
      961 tgcagtctgt gcggtacggg caccggtcag acgtccgttg ccatcaagtg cgagaaaaga
     1021 ttttgcgtac atagtatatg ttcctcagcc gccagacgac acgcaggca
//
LOCUS       DAZSNM010000454         1068 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZSNM010000454 DAZSNM010000000
VERSION     DAZSNM010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.461"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..560
                     /locus_tag="ACHW42_004454"
     CDS             <1..560
                     /locus_tag="ACHW42_004454"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFR8945966.1"
                     /translation="TKGDAMIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDN
                     YQAIQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKS
                     GLTGSVLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMV
                     NEVETALFNARALALKIYSMSGWNL"
     gene            787..>1068
                     /locus_tag="ACHW42_004455"
     CDS             787..>1068
                     /locus_tag="ACHW42_004455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HFR8945967.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          314 a          202 c          257 g          295 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DAZSNM010000455         1058 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZSNM010000455 DAZSNM010000000
VERSION     DAZSNM010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.462"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..948
                     /gene="lpfD-O113"
                     /locus_tag="ACHW42_004456"
     CDS             <1..948
                     /gene="lpfD-O113"
                     /locus_tag="ACHW42_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR8945968.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT          336 a          197 c          212 g          313 t
ORIGIN      
        1 tcgggatatc ttattaccgc gaacgaaaat aaggttggaa cgacatttaa caccactgta
       61 actaatggat cctcatatct tggacgctgt cattgtgata ctggtaacgt aggagaattc
      121 ccctatatct attatacgtc aaaaataaac caggcattaa cttatgctgg ggttcactct
      181 aatattaatt attatgatct taatccgaac cttgatgttg ggattgctat agatattctt
      241 ggggttgggt atgtgaatgc accttttgaa tatcatgcta acaacccgtc tggtaataca
      301 aagtacaatt gcaaccgcat tgaaccttta agtatatcca gtggggctaa agcgatagta
      361 tatttttata tcaagaagac atttgcagga aaattgatta ttcctgaaac aaagatagtg
      421 acattgtatg gaacaattag ccgtgacacc ccggtggatt actcacagcc gatggctgat
      481 gtttatattc ggggcgatat tacagctccg caaagttgcg aaataaataa tttaaagcca
      541 gtttgttttg attttaaaga aataccagct gcagattttt catctgttgt tggaagtgcg
      601 gtaacaacac ataaaattac taaaacagtc actattgagt gtgaaaacct ggggatactg
      661 aataccgatg atatcagtac gtctttttat gctaccgaac ccaatacgga caattcaatg
      721 gtcgtaactt caaactctaa cgtggggata aaaatttacg acaaaaataa taaggaaatc
      781 aaagtgaacg gtggtgagtt gccaacagac atggataaat caacagtgta tggtgaaaaa
      841 tcaggtagtg taactttttc agctgctcct gcgagcctta ctggagctcg cccagccccg
      901 gggcaattta ctgcaacagc gacgataact gttgagattg tgcgctaata ggtctgacaa
      961 cgattgttca tcacatcgtt gtcatctcac ccatttcttt atcactttta aatgcatcat
     1021 gacaaaaaga tggcatgtgg aattttcatc ttcacatt
//
LOCUS       DAZSNM010000456         1048 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZSNM010000456 DAZSNM010000000
VERSION     DAZSNM010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.463"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..725
                     /locus_tag="ACHW42_004457"
     rRNA            <1..725
                     /locus_tag="ACHW42_004457"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            794..870
                     /locus_tag="ACHW42_004458"
     tRNA            794..870
                     /locus_tag="ACHW42_004458"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:828..830,aa:Ile,seq:gat)
     gene            913..988
                     /locus_tag="ACHW42_004459"
     tRNA            913..988
                     /locus_tag="ACHW42_004459"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:946..948,aa:Ala,seq:tgc)
BASE COUNT          262 a          242 c          304 g          240 t
ORIGIN      
        1 gatgtcgact tggaggttgt gcccttgagg tgtggcttcc ggagctaacg cgttaagtcg
       61 accgcctggg gagtacggcc gcaaggttaa aactcaaatg aattgacggg ggcccgcaca
      121 agcggtggag catgtggttt aattcgatgc aacgcgaaga accttacctg gtcttgacat
      181 ccacggaagt tttcagagat gagaatgtgc cttcgggaac cgtgagacag gtgctgcatg
      241 gctgtcgtca gctcgtgttg tgaaatgttg ggttaagtcc cgcaacgagc gcaaccctta
      301 tcctttgttg ccagcggtcc ggccgggaac tcaaaggaga ctgccagtga taaactggag
      361 gaaggtgggg atgacgtcaa gtcatcatgg cccttacgac cagggctaca cacgtgctac
      421 aatggcgcat acaaagagaa gcgacctcgc gagagcaagc ggacctcata aagtgcgtcg
      481 tagtccggat tggagtctgc aactcgactc catgaagtcg gaatcgctag taatcgtgga
      541 tcagaatgcc acggtgaata cgttcccggg ccttgtacac accgcccgtc acaccatggg
      601 agtgggttgc aaaagaagta ggtagcttaa ccttcgggag ggcgcttacc actttgtgat
      661 tcatgactgg ggtgaagtcg taacaaggta accgtagggg aacctgcggt tggatcacct
      721 ccttacctta aagaagcgtt ctttgtagtg ctcacacaga ttgtctgatg aaaatgagca
      781 gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat aagggtgagg
      841 tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga agaggtttta
      901 actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac gcaggaggtc
      961 tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa aatacttcag
     1021 agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAZSNM010000457         1034 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZSNM010000457 DAZSNM010000000
VERSION     DAZSNM010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.464"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..638)
                     /gene="ospC2"
                     /locus_tag="ACHW42_004460"
     CDS             complement(<1..638)
                     /gene="ospC2"
                     /locus_tag="ACHW42_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFR8945969.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGA"
BASE COUNT          329 a          150 c          184 g          371 t
ORIGIN      
        1 gcaccaaaat ccacagtatg atgtgtttta tttagtggga gtgtttcttg atgattggaa
       61 atttcaaccc caaaaaaaac aaaatcgtgg ttagctattt cttcaatatc ctcagagaat
      121 gtgtttttat atgtttgtat attctcagac ttaattttat caaaggactt aatattaaga
      181 gtattatttg acaggttagc gttactttga tgtctgaagt taaaatcaga attaataata
      241 atagagagca agtgacgttc atccgaagaa agaggatgag ttttttgtat tatttcagaa
      301 agatagtgtc tggacacctt atcgaggtgc tcaagtgacc gaaatgatga ccgtgctcca
      361 gatggcgctt caatagaaag atttaatgat ttgaaactgg tcccttgaga gaacatttta
      421 ctataacttt gcgctgctat agacttcctt aaaaagcttt tcacagtatc agcacagtga
      481 tctaaatgtt tttcacttat gccagaagag gatgagtttg ttacgcatga tatgtttttt
      541 tgtaatgctt ttgtatcttt gttcggattt atttttccat caacaagatc aatattgttc
      601 tgaacattaa tatgatttac tgcttcaggt attttcataa aacatccccc attatgtgcg
      661 tttttatctg cagttattta gaataatatt atctaactta atattttctt gtatgtatta
      721 taacgaacat attttaatat gttgtacgat tttcactctg aaatcccctg agtttaatca
      781 cacttccttt actgaaagga attattgcaa cagtattatt tgtttttgga tatatatctt
      841 gcgccttgtt atgtagttaa tgctcagaat atgatagata aaatagagac gcaaaacagg
      901 gcatgctcat aacatgatga taacataaaa aattagtaac aataaatgaa agaatcatgg
      961 gcgctcaaca aaaagagggt aatgctgcca acttactgat ttagtgtatg atggtgattt
     1021 taaggtgctt gcgt
//
LOCUS       DAZSNM010000458         1027 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZSNM010000458 DAZSNM010000000
VERSION     DAZSNM010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.465"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACHW42_004461"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACHW42_004461"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR8945970.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          268 a          235 c          238 g          286 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     1021 tgcgtga
//
LOCUS       DAZSNM010000459         1024 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZSNM010000459 DAZSNM010000000
VERSION     DAZSNM010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.466"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..950)
                     /locus_tag="ACHW42_004462"
     CDS             complement(<1..950)
                     /locus_tag="ACHW42_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR8945971.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPYLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDESMERFSWHVADKGFRMVRYYVF"
BASE COUNT          233 a          306 c          257 g          228 t
ORIGIN      
        1 aaaaaaacgt agtacctcac catacggaac cccttatccg ccacatgcca ggagaacctt
       61 tccatggact catctccact catcaacagg tattcttccc gttttgttcg gtgactgttg
      121 taacgcagac cgatttcatc ctgaccggca taatgctcca gacgactcat cggcaccggt
      181 ggctttttca ggtaagagcc aaagtacacc gccacatggg tggcattatc cattacccgg
      241 gatacgttga cattccagcc acggcggtaa tgcgtgtcca ggaagcgatt ccattcccgt
      301 ttactgcttc cttctgctgc cagcgcatcc ggcatcacca ggtaagggta tttccgtgac
      361 agcaaccgtg ttatccggta gcgccacatg ctcatcacct tacgggcgta aaaatgaaga
      421 tttttccagg tgtggcccga cgtcacacca ccggcagttg tcgataaatg gatatgcgga
      481 tgccactgct ggtcacgccc ccatgtgtgg atcaccgtga atatccccgg ctccacatct
      541 gcctgatggc agatttccag tatcacatcc gctgcaatgc ggctcatctc tgccagtaac
      601 caccggttgt ggaacaccag ggaccagtac tggcagggaa gtgtgaacac aatatgctgc
      661 cacgggcagt aggggaccag gctcagcaga tactgtatcc actgtgcgcc agccttcacc
      721 ccgcagtgcg ggcaggagcg gcttttacac cggaagcaga ccttttttgt atggcaacag
      781 tccggtgatg aacaacacca ctgcgtgtag cccatcagtg tggtcccgca ggccatgatt
      841 tttgtcactg attcaattac caccggacgg actgcccctt ccggttgttt ctccagccag
      901 ttaagccagc ggtttccctg ctgaaaaata tcggcaaaac gggggagcat cagaagggcg
      961 gggcgactcc gtccggccag tgaaccgtgc cacactccgg gcagtacata ccgccggcgc
     1021 tgat
//
LOCUS       DAZSNM010000460         1020 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZSNM010000460 DAZSNM010000000
VERSION     DAZSNM010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.467"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(50..190)
                     /gene="yqcG"
                     /locus_tag="ACHW42_004463"
     CDS             complement(50..190)
                     /gene="yqcG"
                     /locus_tag="ACHW42_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFR8945972.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="ACHW42_004464"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="ACHW42_004464"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR8945973.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          296 a          205 c          237 g          282 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt tcccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt ataagcgtta
//
LOCUS       DAZSNM010000461         1017 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZSNM010000461 DAZSNM010000000
VERSION     DAZSNM010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.469"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..547
                     /locus_tag="ACHW42_004465"
                     /pseudo
     CDS             1..547
                     /locus_tag="ACHW42_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            914..>1017
                     /locus_tag="ACHW42_004466"
     CDS             914..>1017
                     /locus_tag="ACHW42_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945974.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQK"
BASE COUNT          312 a          222 c          215 g          268 t
ORIGIN      
        1 tgggtcgcag attcagttgt ccaggccttc cgaaatcaac cgactgaagg cgctggtcgc
       61 gaaactgcag cgtatgcagt tcggtaaaag ctcagaaaaa cttcgcgcaa aaaccgaacg
      121 gtggatacag gaagcacagg agcgaatcag cgcacttcag gaggaaatgg cggaaacact
      181 gggtgagcaa tatgacccgg tactgccatc ttccctgcgc cagtcttcag cccgtaaacc
      241 gttaccagcc tcacttcccc gtgccccccg ggtcatccgg ccggaagagg aatgctgtcc
      301 ggcctgtggt ggtgaactca gtccactggg atgtgatgtg tcagagcagc tggagcttat
      361 cagcagcgcc tttaaggtta tcgaaaaaca acgccctaaa ctggcctgtc gccggtgcga
      421 ccatatcgtg caggcaccgg tgccttcaaa acccattgca cgcagttatg ccggagcggg
      481 gcttctggcc catgttgtta ccggaaagta tgcagaccat ctgccattat accgccaaag
      541 tgatctacta ttccacacag ctatttagct ctctttttgt tgagctccca tgattctttc
      601 atttattgtt actaattatt ttatgttatc atgatgttag aacacgctct gctctgtgtc
      661 tctattttat ctatcatatt ctgaggatta actacataac aaggcgcaag atatatatac
      721 aaaaacaaat aataccgttg caataattcc ttttagtaaa ggaggtgtga ttaatttcag
      781 gggatttcag agtaaaaatt gtacaacata ttaaaatatg ttcatttcgt tataatacac
      841 acaaggaaat attaagttag ataatatatt ctaaataacc atagataaaa acgcacataa
      901 tggggaatat tttatgaaaa tacctgaagc agtaaatcat attaatgttc agaataatat
      961 tgatcttgtt gatggaaaaa cgaatccgaa taaagcaaca aaagcattac aaaaaat
//
LOCUS       DAZSNM010000462         1013 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZSNM010000462 DAZSNM010000000
VERSION     DAZSNM010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.470"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..135
                     /gene="yecU"
                     /locus_tag="ACHW42_004467"
     CDS             <1..135
                     /gene="yecU"
                     /locus_tag="ACHW42_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR8945975.1"
                     /translation="STADITLKKQPLLFLAKLMVYSAALTFFTTNFHCNMTRKINEYA
                     "
     gene            122..346
                     /gene="yecF"
                     /locus_tag="ACHW42_004468"
     CDS             122..346
                     /gene="yecF"
                     /locus_tag="ACHW42_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR8945976.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(379..999)
                     /gene="sdiA"
                     /locus_tag="ACHW42_004469"
                     /pseudo
     CDS             complement(379..999)
                     /gene="sdiA"
                     /locus_tag="ACHW42_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
BASE COUNT          288 a          252 c          218 g          255 t
ORIGIN      
        1 agcactgccg atatcacgct caaaaaacaa ccactgctat ttttagcaaa gcttatggta
       61 tactccgccg ccttaacatt tttcaccaca aattttcatt gcaacatgac gaggaaaata
      121 aatgagtacg cctgattttt ctactgccga gaataatcaa gaactggcaa atgaagtctc
      181 ctgcctgaaa gcgatgctga cgctgatgct gcaggcgatg ggacaagctg acgcgggccg
      241 cgtgatgtta aagatggaaa aacagcttgc gctgatcgaa gacgaaaccc aggctgcagt
      301 attttccaaa acggttaagc aaattaaaca agcctaccgt cagtaatata aaaccggctg
      361 agagcgtgcc tttcagccgg ttattgacaa aatcatcgct atctctgctg atgtcttccc
      421 ttccgccgtc catttcagaa tttctttttc gcgcttgctg aaattcatct ctggcgtcat
      481 cactatttca tcatttaaac gcatcagagc catcagactt tcgcgcacca gtaactgcat
      541 ttttaattgc agttcatcac taagaatggg tatttcgcgc gtgctgcaac gggaaaagga
      601 caaaaagccc agcgcccgat ttggcagcat taaatactga gtgacaccgc ggcgtaaacc
      661 atgtgcgtgc gcggcttccc ataatggctg tgcttcgctg aataagtcat cattccacat
      721 taaatggccc tgactaaagt tttcagggtt cagcacagga tcaattgcga gaaaattttt
      781 tgcctgataa taactaaccc acgactcagg gtaattggtg tagaaagcca ctttaggtcg
      841 cgtgaatggt accgggtggc gaacacataa cgaatagtaa tcgtactcca gctgctgagc
      901 ctgaagctct atttcatggt aggcctcttc tgcggcctcc atcctctgaa aacgcaacag
      961 catcgtgcga cgccagctga aaaaatccgt atcctgcata gtaaaccgca acg
//
LOCUS       DAZSNM010000463          991 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZSNM010000463 DAZSNM010000000
VERSION     DAZSNM010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.471"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..448
                     /gene="yhaJ"
                     /locus_tag="ACHW42_004470"
     CDS             <1..448
                     /gene="yhaJ"
                     /locus_tag="ACHW42_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFR8945977.1"
                     /translation="APDMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTR
                     VKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKD
                     IAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFAGK"
     gene            complement(655..>991)
                     /locus_tag="ACHW42_004471"
     CDS             complement(655..>991)
                     /locus_tag="ACHW42_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR8945978.1"
                     /translation="VGFRGRARRKEYWMFILVNIIFTFVLGLLDKMLGWQRAGGEGIL
                     TIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFNCQAGTPGENRF
                     GPDPKLEQE"
BASE COUNT          252 a          253 c          261 g          225 t
ORIGIN      
        1 cgcgccggat atgcattttc gttcctcgtc ggagatcaac tcgcgcaagc tctatacgtt
       61 aatgaacgtc tacgttgccg cgcctgatca cccgattcat caggagccgg aaccgttatc
      121 tgaagtgacg cgtgtgaaat atcgtggaat tgcggtagcg gataccgctc gtgagcgccc
      181 ggtgttgacc gtacagctgc tggacaaaca gccgcgctta acggtgagca cgattgaaga
      241 taaacgtcag gcattactgg cggggcttgg cgtggcgacg atgccgtatc cgatggtcga
      301 aaaagatatt gcggaagggc ggttgcgtgt cgtcagcccg gaatcgacca gcgagatcga
      361 tattattatg gcctggcgtc gtgacagtat gggggaagcg aaatcctggt gtctgcggga
      421 aattccaaag ttgttcgctg gaaaataatc tattaagcac ctttcacaag gtgcttctga
      481 ctgctgacaa acgcaaaact gcctgatgcg ctacgcttat caggcctacg cagtcactgc
      541 aatatattga attttggcaa tttgtaggct ggataaggcg tttacgccgc atccggcata
      601 aacaaagcgc actttgtcaa caatttgaag caccttttac aaggtgctta atcattattc
      661 ttgttccagt tttggatctg gtccaaagcg gttttcgcct ggcgtacccg cctggcagtt
      721 aaagacgatg ataataagcc agccgataaa cgggattaag aatagcagtg cccaccacgc
      781 cgagcggtcg gtgtcgtgca ggcggcggaa ctgaaccgcc caccacggta aaaacaccag
      841 aataccgtag attatcgtca ggatgccttc gccaccagcg cgttgccagc ctaacatttt
      901 atccagcagc cccagcacga acgtaaagat gatgttgacc agaataaaca tccagtactc
      961 tttgcgccgc gcacgacccc ggaaaccgac a
//
LOCUS       DAZSNM010000464          971 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZSNM010000464 DAZSNM010000000
VERSION     DAZSNM010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.474"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>971
                     /locus_tag="ACHW42_004472"
     CDS             <1..>971
                     /locus_tag="ACHW42_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR8945979.1"
                     /translation="NIPFDVLQGGASLINAISQGLGGPKVLDDVYRPVDRPTDPYAQA
                     GESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAGLSIATHGLINGVTRGV
                     RGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAAEARVAQGVPMTPATKN
                     PEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDSLLPSHFSGNEQYKAVE
                     QAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGMSDKFIDTVNGRMSALM
                     KRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNLDPIEK"
BASE COUNT          278 a          216 c          260 g          217 t
ORIGIN      
        1 aaacatacca tttgacgtat tgcagggtgg cgcaagtctg attaatgcaa tcagtcaggg
       61 gcttgggggg ccaaaagtat tggatgatgt ttatcgtcca gtagacagac cgacagaccc
      121 ctacgcgcaa gctggagagt caataggcgg ttatcttgtt cctggagcag gtgtagctgg
      181 aaacatggtc attggttctc tcgctgacgc ggcgaatcaa cggggtgatt ttgccgaaaa
      241 tgccgctatt aatgccggac ttagcattgc tacgcatggc ctgataaatg gcgttacccg
      301 tggtgttcgt ggtgcatcaa atataattag tggcaataaa acatctgcac agagagcgac
      361 cactgcgcca acagaaacat caccattctc cggtgatgcc gctgcagcaa caaatcctgc
      421 ggttcatgcc gcagaggcaa gagtagcaca aggtgtacca atgacgcctg cgacgaagaa
      481 cccagaggaa gtcgttcgca cagtagcagc acaaaaaagg ccaaatctcg cttcatcgct
      541 tgatgaacta gatatcaatc ctcaggctga agttctggag tctgctgaaa ggcttaatgt
      601 tgattcatta ctcccttcac acttttccgg gaacgagcaa tacaaggcag ttgagcaagc
      661 aatcaagtcc cgtgcgggtt ctgctctaca ggtgcaggaa aatgaagcaa tcaggcagct
      721 agcacagggc gcgggtgaga taattgatcg cgtttccggt gcaaaagatg ctcttggtat
      781 gagcgacaag tttattgata cggtcaatgg aagaatgtct gcgctgatga aacgaagcga
      841 ccagctttat cgcaatgttg aaaaggcgat gcctgcaggt gcaaaaattg atgcgccatc
      901 aacaaggtca atgctcaaac aggtggcaga agatcttggc gggatgaaaa accttgaccc
      961 tattgaaaag a
//
LOCUS       DAZSNM010000465          932 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZSNM010000465 DAZSNM010000000
VERSION     DAZSNM010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.475"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(55..243)
                     /locus_tag="ACHW42_004473"
     CDS             complement(55..243)
                     /locus_tag="ACHW42_004473"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945980.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            complement(540..778)
                     /locus_tag="ACHW42_004474"
                     /pseudo
     CDS             complement(540..778)
                     /locus_tag="ACHW42_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          245 a          181 c          216 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aa
//
LOCUS       DAZSNM010000466          922 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZSNM010000466 DAZSNM010000000
VERSION     DAZSNM010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.476"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..199
                     /locus_tag="ACHW42_004475"
                     /pseudo
     CDS             1..199
                     /locus_tag="ACHW42_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            299..>922
                     /locus_tag="ACHW42_004476"
     CDS             299..>922
                     /locus_tag="ACHW42_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR8945981.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          292 a          217 c          205 g          208 t
ORIGIN      
        1 ttggctacgt aattaaaaca gaaaataaag aaaccccatt cactgtagtt gatatcgacg
       61 gtctatcagg aaactttaaa actcttgttg aaggtgccac aaaaatgtgc ctagtttaca
      121 tcggaaataa tctgcccgca gaaaaaaagc cgaatttctg gcaaatctga ttgcaatgaa
      181 attaaaaggt gaaatctgaa aaaaagaaag cctgcacagt gtgcaggcct gagtgaagaa
      241 cctgggacat ttattcatca ctcgcagtaa ttttaatctg agttgaggtt aaaaaacaat
      301 gagcacaaaa ccactcttcc tgttacggaa agcgaaaaaa tcatccggtg aacctgacgt
      361 cgtcctgtgg gcaagcgacg attttgaatc gaccagcacc actctggact atctgctcgt
      421 taagtcaggt aaaaaactga gcaactattt taaagctgtt gccacaaatt ttcctgtcgt
      481 taatgacctt ccccctgaag gtgagatcga ttttacctgg agtgaacgct atcaactcag
      541 caaagactcc atgacatggg aactaaaacc gggagcagcg ccagacgacg ttcaccacca
      601 ggataatgct caagaaacca aagaactggc gggaggccag gaagaaaacg cgcaggcaga
      661 cgcccacgag gattgccagg attgcgaagt ctctgtagcc actttgcggt tcacacagcg
      721 tcttctgcac atttttacgt atgctgccgg ggatctgaaa tacctgcatc atgccacccg
      781 tgaacaacgc aaacacatta ctgctcttga gatggatcag gaaaacagct atgtccagaa
      841 tctgctgttg gccatacgcg gcatggcaga accgacaact ctggataatg ccgccctgct
      901 ccgcctgact gatgcaatta ag
//
LOCUS       DAZSNM010000467          915 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZSNM010000467 DAZSNM010000000
VERSION     DAZSNM010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.477"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..422
                     /locus_tag="ACHW42_004477"
     CDS             <1..422
                     /locus_tag="ACHW42_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR8945982.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            470..790
                     /gene="cyaY"
                     /locus_tag="ACHW42_004478"
     CDS             470..790
                     /gene="cyaY"
                     /locus_tag="ACHW42_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR8945983.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
BASE COUNT          260 a          222 c          230 g          203 t
ORIGIN      
        1 aattccatct gattactggc aatattgcgc aatataaaga tcggctgtat gtaacaaaat
       61 tatcgacatt tggtgaagac cagcttgaga taatcccgct ggatacgcca gacctggtca
      121 ttgatcgctc atttaatagc ggcaaacagc atgcttactt tatccgccaa ttacggtcaa
      181 agagcttgca aattattcca gttaacggtt cgctaactaa aaacgatcgc ttcgcttatg
      241 acgatcgcaa tgtttataca tggaccgata cagaggtaag gattacgccc tccccctgcc
      301 cggcgaaaac tcgtgtcaga gaggagaacg tacgtgaagt tcaaaacaga gacatcatta
      361 ttccggtgac ggatgaatca tgccggaacg cagcagcaga ggtgcaaact ttgaagccct
      421 gaccccatgc cgctatactg ccgccatcac ttaaaaacag gatacaacaa tgaacgacag
      481 tgaatttcat cgcctggctg atcaactgtg gctgaccatt gaagagcgtc tggacgacag
      541 ggatggcgac agcgatatcg actgcgaaat caacggcggc gtactgacca ttacctttga
      601 aaacggcagc aaaatcatta ttaaccgcca ggagccgctg caccaggtgt ggctggcaac
      661 caaacagggc ggctaccatt ttgacctgaa aggcgatgaa tggatttgcg atcgcagcgg
      721 cgaaaccttc tgggatttgc tggaacaggc ggcgacgcaa caggcgggtg aaacagtcag
      781 tttccgctaa gattgcatgc cggataagcc tcgctttccg gcacgctcat cacgaaaaat
      841 actgctgtaa tagcggcgta tcgtgatcct gattggcggg cggcaggttg ccaatcgatt
      901 ttgttcggaa cggaa
//
LOCUS       DAZSNM010000468          911 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZSNM010000468 DAZSNM010000000
VERSION     DAZSNM010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.478"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>911
                     /locus_tag="ACHW42_004479"
     CDS             <1..>911
                     /locus_tag="ACHW42_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HFR8945984.1"
                     /translation="DYANNVIIILPSGIAPDYLEQYSRFELRLRKHLSLAEESLWQKC
                     AQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQHLLGQKKLPSLEWLKIVATDEHI
                     LASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPYAASDYCADVLRHINHP
                     FALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQKEENSWRIMLMTMFISQ
                     PALAEQVIPWLSTPAVAVLKSCQHQLTQPSNHASADLLPAIVVSPPWISKKKKSPIPV
                     LDLAPLGIEPICYLTEEISNQLLAKYI"
BASE COUNT          260 a          228 c          193 g          230 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 atctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat c
//
LOCUS       DAZSNM010000469          901 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZSNM010000469 DAZSNM010000000
VERSION     DAZSNM010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.479"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            103..>901
                     /locus_tag="ACHW42_004480"
     CDS             103..>901
                     /locus_tag="ACHW42_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8945985.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVHVAGNPLSGHTMRTLQQITTGPDYSGP"
BASE COUNT          306 a          181 c          156 g          258 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcatc
      661 cccccctttc cagataagct tgaagggctg gctctggcta ataattttat agaacaacta
      721 ccggaattac cttttagtat gaacagggct gtgctaatga ataataatct gacaacactt
      781 ccggaaagtg tcctgagatt agctcagaat gccttcgtac atgttgcagg taatccattg
      841 tctggccata ccatgcgtac actacaacaa ataaccaccg gaccagatta ttctggtcct
      901 c
//
LOCUS       DAZSNM010000470          895 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZSNM010000470 DAZSNM010000000
VERSION     DAZSNM010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.480"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..304
                     /locus_tag="ACHW42_004481"
     CDS             <1..304
                     /locus_tag="ACHW42_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HFR8945986.1"
                     /translation="LRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHE
                     FYEAELKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(510..791)
                     /locus_tag="ACHW42_004482"
     CDS             complement(510..791)
                     /locus_tag="ACHW42_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945987.1"
                     /translation="MQNRKWILTSLVMTFFGIPILTQFFAAVIAMLGAGLAAIPEVCN
                     LLFTPTIYLLLNIFMLTLGALMLFFSGRVGNVPEFCYVGYDGVGFAVIS"
BASE COUNT          253 a          227 c          210 g          205 t
ORIGIN      
        1 cctgcgccgc cgttacccgt tcctgactga atcgctggca cgtcactaca cgcgcactta
       61 cggcagcaac agcgagctgc tgctcggcaa tgcgggaacg gtaagcgatc tcggggaaga
      121 tttcggtcat gagttctacg aagcggaact gaaatacctg gtggatcacg aatgggtccg
      181 ccgcaccgac gatgccctgt ggcgtcgcac aaaacaaggc atgtggctga atgcggatca
      241 acaatctcgt gtgagtcagt ggctggtgga atatacgcag cagaagttgt cgctggcgtc
      301 gtaaattaac gtaaggtgat caggtcagat ttcaatctgg cctgagactg atgacaaaca
      361 caaaactgcc tgatgcgcta cgcttatcag gcctacgtgg tttatgcaat atattgaatt
      421 tgcatggtct tgtaggccag ataagacgtt cacgtcgcat ccggcatgaa caaagcgcac
      481 tttgtcaaaa atctaaccta ctttttaatt caggaaatta ccgcaaagcc cacgccatca
      541 tacccaacgt aacaaaactc aggcacgttc cccactcgcc ccgagaaaaa tagcattaat
      601 gcgcccagcg tcagcataaa aatattgagc agaaggtaaa ttgttggcgt aaagagtaaa
      661 ttgcaaacct caggaatagc ggcaagccca gcccccagca tggcaataac cgctgcaaaa
      721 aattgcgtca gtattgggat gccgaaaaaa gtcattacca gcgaggtcaa aatccatttt
      781 ctattttgca ttattctttc cattctttct gaatggcgaa attatactcc ccgagtcccc
      841 ttgccccttc tggacacttt tccgaaatga tggcgaaaaa aacgggaccc tttgg
//
LOCUS       DAZSNM010000471          884 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZSNM010000471 DAZSNM010000000
VERSION     DAZSNM010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.481"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>884)
                     /gene="ospC4"
                     /locus_tag="ACHW42_004483"
     CDS             complement(<1..>884)
                     /gene="ospC4"
                     /locus_tag="ACHW42_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HFR8945988.1"
                     /translation="QSNSNLSNNILNIKSFDKIQSENIQTHKNTYSEDIKEISNHDFV
                     FFGVEISNHQEKLPLNKTHHTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEH
                     QSFFLDNFKEVVDEVSRYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFRE
                     FCYNKNIDPVSLDRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEIN
                     NKVTDKKMAHQALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEY
                     LLSKDGANYKVLEYFINN"
BASE COUNT          284 a          143 c          126 g          331 t
ORIGIN      
        1 cattattgat aaaatattct agcactttat agttggcacc atctttacta agtaagtatt
       61 caacatcata cagattacaa tatctattgt ttttcatttt ctccatctct gcgacatctt
      121 gttttgtaaa attaaattta gatagtaaat aaagtgctat atcagctttt ttattgccta
      181 aagaataagc aagggcctga tgagccattt ttttatcagt cactttattg ttaatctctt
      241 cataattaga tgctttaatc gcatcctcca aagatatgtc tctgagtttt atttttttga
      301 agttatctgt acttagcatt cttggtatat gatactcaag ctgaaacaca aagtttataa
      361 ttctatccag actaacagga tcaatatttt tgttataaca aaactctctg aatccttgat
      421 ctttactctt tcttataaaa tcaataagat gcaacccaat tccaagcctc atatctttag
      481 tgttaaatat tggaacatca gttttcccct gattaccatg cacatacctg ctaacttcat
      541 ccacaacctc tttaaagtta tctaagaaaa atgattggtg ctcatggtaa aaaacaggtg
      601 gaatagcatt atcaaagtga tcggttaatg tcatatatcc atatggagag tcatgatcaa
      661 tgatatacgc attggcgcca aaatctacag tatgatgtgt tttatttagt gggagttttt
      721 cttgatgatt ggaaatttcg accccaaaaa aaacaaaatc gtggttagag atttctttaa
      781 tatcctcaga gtatgtattc ttatgtgttt gtatattttc agactgaatt ttatcaaagg
      841 atttaatatt aagaatatta tttgacagat tagagttact ttga
//
LOCUS       DAZSNM010000472          875 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZSNM010000472 DAZSNM010000000
VERSION     DAZSNM010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.482"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          274 a          176 c          157 g          268 t
ORIGIN      
        1 aaatcaacat aaaaaattca gcaaaattta atatttgtta aactagccgt ctcaaatatg
       61 acattattag ttaactgcat ttgccacaaa atggctattt catggaacgc agtaatttct
      121 tagtggctat tatcatgcta attcttagat ccctagcgat tattcctgcc tggttcgtca
      181 gcttaggcca tgagattgtt acattccttt ttatcctgga gatatggggg aacgcaggcg
      241 acgctttttc atctacatcc gttggtaaat atagctttgg tcactacgac ttaagccttc
      301 ctcaatcaca gctattcgcc gggcgatacg gcaaacaaac taagtgaaac atcatcctga
      361 aaacaccaac atcaacaagc ctctccagat cgacatcagg agtgaccagt tacaagccac
      421 aaaccaagaa acatatttca tgatgagaat tatgctcagc ctgatggcga gtaaaccctt
      481 attttcacca ctctcactga gttagcttgc aaagctttct ttgtttagat tctaaataaa
      541 ttatttataa aacgtatagt taaaagaaaa tctttagttt ttgaagacca ccgcaagtgt
      601 tcgtctggct tcacatggca tcttcttctt agaaaaagat cgacatattt tgtgacgcga
      661 attgcaaatc tggttttgtt gtatggattg cgtgattttt gatctggtat aacaggtata
      721 aagatgcacc acgatagtca atgagactgg gcatctcgta atctatggca aacatcactt
      781 cagttctttc acatcgggtg atgaaaatgc acttcagtct gaaaggaata tgaaaatgag
      841 atcaacagac actctatttt atgactctgg gtaaa
//
LOCUS       DAZSNM010000473          870 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZSNM010000473 DAZSNM010000000
VERSION     DAZSNM010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.483"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>870
                     /locus_tag="ACHW42_004484"
     CDS             <1..>870
                     /locus_tag="ACHW42_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR8945989.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIV"
BASE COUNT          263 a          153 c          209 g          245 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga
//
LOCUS       DAZSNM010000474          851 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZSNM010000474 DAZSNM010000000
VERSION     DAZSNM010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.484"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            59..556
                     /locus_tag="ACHW42_004485"
                     /pseudo
     CDS             59..556
                     /locus_tag="ACHW42_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            597..788
                     /locus_tag="ACHW42_004486"
                     /pseudo
     CDS             597..788
                     /locus_tag="ACHW42_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          219 a          210 c          250 g          172 t
ORIGIN      
        1 gcgccagccc cgtcccgccc gatagagcgc ggcatagcgg gccccggatt acttgcgtgc
       61 gacggcagaa gaagccctga agcgaatcag tgaactgtac gccatcgagg atgaaatacg
      121 gggattacca gagtcagagt gtcttgcagt caggcagcag cgaagcaaag cgttactgac
      181 gtcgctgcat gaatggatgg tggagaagaa tggcacgctg tcgaaaaaat ccagactggg
      241 cgaagcattc agctatgtac tgaatcagtg ggacgccctc tgttattaca gtgatgacgg
      301 tctggcggag gcggacaata acacagcgga aagagcgctt cgtgcagtct gtctcggaaa
      361 gaaaaattac gtgttcttcg gtagcgatca cggcggcgag cgtggtgcac tgctgtacgg
      421 gctgatcggc acctgccgtc tgaacggtat cgatccggaa gcgtatctgc gccatattct
      481 gagcgtactg ccggaatggc cttccaaccg agttgacgac ctcctgccat ggaaggtggt
      541 gctaccatca ggataatcgt caatacggtg ttcggtgaac gtttacttgg agcatcatgc
      601 ttcatgaaga aaaactggca cgtcatcaac gtaaacaggc gatgtatacc cgaatggcag
      661 ccttcccggc ggtgaaaatg ttcgaagagt atgacttcac gttcgccacc ggagcaccgc
      721 agaagcagct ccagtcgtta cgctcactca gcttcataga gcgcaatgaa aacatagtct
      781 tactggggac atctgacatc acaaatcccc gggtggggat ttgtgtataa gagacagccc
      841 atggaggaag g
//
LOCUS       DAZSNM010000475          845 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZSNM010000475 DAZSNM010000000
VERSION     DAZSNM010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.485"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..480
                     /gene="virA"
                     /locus_tag="ACHW42_004487"
     CDS             <1..480
                     /gene="virA"
                     /locus_tag="ACHW42_004487"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR8945990.1"
                     /translation="IINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTSAFWQSLHIT
                     SSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAISASGGRAYE
                     VSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVSFAIISPDV"
     gene            659..>845
                     /locus_tag="ACHW42_004488"
     CDS             659..>845
                     /locus_tag="ACHW42_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8945991.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT          264 a          194 c          161 g          226 t
ORIGIN      
        1 ataatcaatt caaacttccc ccaacaagac ctatgttttc aaacagaaaa acttttgttt
       61 acttcactct ttcaagaccc tgcatttata tctgcactaa catctgcatt ctggcaatct
      121 cttcacatca cgtcttcctc tgtggaacac atatatgccc aaattatgtc cgaaaacata
      181 gaaaacaggc ttaatttcat gcctgaacaa cgagttatta acaattgtgg acatattata
      241 aaaatcaacg cagttgtccc taaaaacgac actgcaatct ctgcctctgg ggggagggct
      301 tacgaagtta gctcatcaat attaccatcg cacataacat gtaatggtgt tggtattaat
      361 aaaatagaaa caagctatct cgttcatgct ggaacacttc caagcagtga agggcttcgc
      421 aatgctatcc ctccagaaag tcgtcaagta tcatttgcca tcatatctcc tgatgtttaa
      481 gccaatatta tattggctgc cagactgtaa aaaaacgttc ttcagaacag ctctataaac
      541 tattaaacca aagctaaaag gatggggacc aatagaatct cagcccatcc tttttatatc
      601 attatgaacc gccccggaaa tcctggagac taaactccct gagaaagagg taaacaggat
      661 gactaaaaat acacgttttt cccctgaagt ccgtcaacgg gcagttcgta tggttctgga
      721 aagtcagggc gaatatgact cacaatgggc agcgatttgt tccattgccc caaagattgg
      781 ctgtatactg gaaactctgc gtgtctgggt acgccagcat gagcgggata ccgggggcgg
      841 tgagg
//
LOCUS       DAZSNM010000476          844 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZSNM010000476 DAZSNM010000000
VERSION     DAZSNM010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.486"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(110..577)
                     /locus_tag="ACHW42_004489"
     CDS             complement(110..577)
                     /locus_tag="ACHW42_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR8945992.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..>844)
                     /locus_tag="ACHW42_004490"
     CDS             complement(643..>844)
                     /locus_tag="ACHW42_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HFR8945993.1"
                     /translation="QCFARFAKDERGVTAIEYALIGVAMATLLAFIFGDQNSGFLGAI
                     KDAFDAIAAAIQQVTISGTSNP"
BASE COUNT          298 a          184 c          165 g          197 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgt
//
LOCUS       DAZSNM010000477          840 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZSNM010000477 DAZSNM010000000
VERSION     DAZSNM010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.487"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..79
                     /locus_tag="ACHW42_004491"
     CDS             <1..79
                     /locus_tag="ACHW42_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8945994.1"
                     /translation="VERRVKERMILSRNRNYSRLATASP"
BASE COUNT          224 a          255 c          182 g          179 t
ORIGIN      
        1 agttgagcgt cgtgtgaagg agcgcatgat tctgtcacgt aaccgtaatt acagccggct
       61 ggccacagct tccccctgaa agtgacctcc tctgaataat ccggcccgcg ccggaggcat
      121 tcgcacgcct gaagcccgcc ggtgcacaaa aaaacagcgt cgcatgcaaa aaacaatctc
      181 atcatccacc ttctggagca tccgattccc cctgttttta atacaaaata cgcctcagcg
      241 acggggaatt ttgcttatcc acatttaact gcaagggact tccccataag gttacaaccg
      301 ttcatgtcat aaagcgccag ccgccagtct tacagggtgc aatgtatctt ttaaacacct
      361 gtttatatct cctttaaact acttaattac attcatttaa aaagaaaacc tattcactgc
      421 ctgtcctgtg gacagacaga tatgcacctc ccaccgcaag cggcgggccc cgaccggagc
      481 cactttagtt acaacacaca aaaacaacct ccagaaaaac cccggtccag cgcagaaccg
      541 aaaccacaaa gcccctccct cataactgaa aagcggcccc gccccggccc aaagggccgg
      601 aacagagtcg cttttaatta tgaatgttgt aactacatct tcatcgctgt cagtcttctc
      661 gctggaagtt ctcagtacac gctcgtaagc ggccctcacg gcccgctaac gcggagatac
      721 gccccgactt cgggtaaacc ctcgtcggga ccactccgac cgcgcacaga agctctctca
      781 tggctgaaag cgggtatggt ctggcagggc tggggatggg taaggtgaaa tctatcaatc
//
LOCUS       DAZSNM010000478          840 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZSNM010000478 DAZSNM010000000
VERSION     DAZSNM010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.488"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..777)
                     /locus_tag="ACHW42_004492"
     CDS             complement(<1..777)
                     /locus_tag="ACHW42_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFR8945995.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVK"
BASE COUNT          243 a          191 c          150 g          256 t
ORIGIN      
        1 tttaacgcga tccagcctgg tttttgcaac gaaagcatcg atttgggcaa cgtcttcccc
       61 gtgattgata atgttaatct gattaaattt cgactcgctt tcaccaaccg gaaatttctt
      121 ttcttctaat cgcgccagag aatcaatggt aaaacttttt gccgttactt tgttcattgg
      181 tgataattgc agaacaatat cttcgacttt gccttcaata tccaggttag acaatgaact
      241 accatcacca ttaaaagtaa actggccctc accaaaagaa aattttccat actctgcagg
      301 ggcaacatct aatgtcgtag agaatgagcc accaaacgcg aatcgcaatg tcgcttccac
      361 gggcgattta tttttcgcgg cgataaatag cggttgtgtc agttcattat tcactaagtt
      421 cacttttgcc gctgccagtg ctgggatgac attaccatgc attaacattg tgatgggcaa
      481 cggaccgtgc tcaacatcca catcaaatac aactttctgg cctggcttga tattgagatc
      541 gggtgcaccg ttatcgaatg tcatttgcat ttgaaaacgg gaattaaaaa agcctttgtc
      601 aaagttttta taacttaacg tcatatcgta ggcatgttca cctttctttt tcgcatcgtt
      661 aaaatcttta ataaactttt cgataccagg ctgaatttgc gtacctgtgt accatgtgcc
      721 accaccccag attacggcta acgcaacaat aacacctgta gctgacttac gtatcatata
      781 aatattccct gataaaacag caaaactttg aatatccact tatgctgaaa taatgtccag
//
LOCUS       DAZSNM010000479          838 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZSNM010000479 DAZSNM010000000
VERSION     DAZSNM010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.489"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(614..>838)
                     /locus_tag="ACHW42_004493"
     CDS             complement(614..>838)
                     /locus_tag="ACHW42_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR8945996.1"
                     /translation="GNDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPIC
                     AIIYTCICVYVSDKIGHWLSRTVGGPFLIK"
BASE COUNT          243 a          159 c          157 g          279 t
ORIGIN      
        1 taaatagctg cgcagaaaag tgctccactt tcgctcagcc cttgctgggc gaagcatcag
       61 gaaggcgttc tcagctaggt tgtgttatat ctggcttgag ttttgttgcc catacgcaac
      121 acattaccca tctgtaaacg acaagtgtgg cagaattagg tgcagctatt tagactatat
      181 cgacgcagta acgataatag atatcgaaac tgtactagat attagctact tatggttacc
      241 ggggggagtc ttttgagcac tggtgttatc aattggcttc aaataattcg ccaataaatt
      301 atattgcgat agctcatcac cttccaggtt gtttatagcc agtttatcag cgtcaccacc
      361 tactgtttat gcttttattt ctttgattaa taagtatatg atattttaat tcatctggtg
      421 tatttctttc tcatgtgttg gtcgactgaa tatctgttca gtgaaggggt aattttatgt
      481 ttgcacttcg aacattccct tcagttgcta ggaaagaaaa ccgtaacatt tgcgcattaa
      541 ttttaacagt ataatctgag cataaatatg cgctatatat ctataaaaag gcgcaattaa
      601 ttgcgccttt ttatcatttt atcaaaaatg gcccaccgac tgtcctcgat agccaatggc
      661 caattttatc tgatacatat acacaaatac aggtatatat gattgcgcag ataggatttg
      721 gaatagtcac gctgaagcct ataattgcta aagccatagg gataaataat ttggtgatat
      781 actccattcc acatatgtta agagtatttc ttccagctct tacaataaag tcatttcc
//
LOCUS       DAZSNM010000480          833 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZSNM010000480 DAZSNM010000000
VERSION     DAZSNM010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.490"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..328
                     /locus_tag="ACHW42_004494"
     CDS             <1..328
                     /locus_tag="ACHW42_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR8945997.1"
                     /translation="VSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNG
                     LLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVI
                     LKITYN"
     gene            complement(371..571)
                     /gene="glgS"
                     /locus_tag="ACHW42_004495"
     CDS             complement(371..571)
                     /gene="glgS"
                     /locus_tag="ACHW42_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR8945998.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          185 c          155 g          241 t
ORIGIN      
        1 cgttagtgag acgtttagtg tagtcatgac aaaagatcct ggtgcggcct gtactgagca
       61 attcaatatt ttagggagct ttttcactac ggatattttg agtgattata gccatctgga
      121 tatgggtaat ggactgctat tgaagatatt tcataaagat ggaacagcaa cggaatttaa
      181 ccgcttctca caatttgctt ctttttcatc gtctagtgca ccttcggtca ccgcaccatt
      241 cagggcagaa cttagtgcga acccggcaga gactgttgtt gagggaccgt ttagtaaaga
      301 cgtaatcctc aaaatcacct ataactagca tctaatacaa acactaaaac gggccatcag
      361 gcccgttata tcagtgctct aactccagct ctcttgcctg catcatctgt tggcaatacc
      421 aggcataacg tgcgcaaaac caagttctat gttcttcatc catgttacca gtaacagcca
      481 gaatatcgac cgggcgacct tctgcgtgca ttctggcaaa actacgcgcc aggaaatcga
      541 aattattcaa agaattaaga ctatgctcca taagcacatc ctccggttat cgcatacctt
      601 ttaaggccat atgctttttc tgatttcagt attccccaat cagtgtagtg cctttaaaca
      661 taacgtgcgt aaatatattg tccccgatca cactttttag tgaaaaataa aaaaccatat
      721 taaaatactt tctcttccag aacttctcta acatgtgtta ttattaacaa agacaaatgt
      781 aagcagggaa actattccaa ggtaaatatt gcgtcactaa atggacattg gag
//
LOCUS       DAZSNM010000481          829 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZSNM010000481 DAZSNM010000000
VERSION     DAZSNM010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.491"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..460)
                     /gene="soxR"
                     /locus_tag="ACHW42_004496"
     CDS             complement(<1..460)
                     /gene="soxR"
                     /locus_tag="ACHW42_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR8945999.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQ"
     gene            546..>829
                     /gene="soxS"
                     /locus_tag="ACHW42_004497"
     CDS             546..>829
                     /gene="soxS"
                     /locus_tag="ACHW42_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR8946000.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRR"
BASE COUNT          201 a          235 c          182 g          211 t
ORIGIN      
        1 tttgttcatc ttccagcaag cgtgcgccgg taccttcttc tcctaagcgg tcgcccgggt
       61 tacgcaacgg gcaatcactg cgcgaaaggc agccacaacc aatacatccg tccagttcgt
      121 cacgcagcgc cactaaggta tgaatgcgcc gatccaactc ttctcgccat tgggacgaaa
      181 gctgtttcca ctctttcgca cttaacgtat gcccttctgg caacacgcca aacgcttcac
      241 caatggtcgc cagcggaatg ccaatacgct gagcgatttt gataattgcg acatatcgca
      301 acacatcacg tttatatcgc cgctgattgc cgctgttacg gatactagta atcaaccctt
      361 tgctttcata gaaatgcagc gccgataccg ccacaccgct gcgtttcgcc acttcgccag
      421 gggttagcag cgctttaatg cggggtaatt tcttttccat aaatcgcttt acctcaagtt
      481 aacttgagga attatactcc ccaacagatg aattaacgaa ctgaacactg aaaagaggca
      541 gatttatgtc ccatcagaaa attattcagg atcttatcgc atggattgac gtgcatattg
      601 accagccgct taacattgat gtcgtcgcaa aaaaatcagg ctattcaaag tggtacttgc
      661 aacggatgtt ccgcacggtg acgcatcaga cgcttggcga ttacattcgc caacgccgcc
      721 tgttactggc cgccgttgag ttgcgcacca ccgagcgtcc gatttttgat atcgcaatgg
      781 acctgggtta tgtctcgcag cagaccttct cccgcgtttt ccgtcggca
//
LOCUS       DAZSNM010000482          828 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZSNM010000482 DAZSNM010000000
VERSION     DAZSNM010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.492"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..154
                     /locus_tag="ACHW42_004498"
     CDS             <1..154
                     /locus_tag="ACHW42_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR8946001.1"
                     /translation="VLKAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVKVK
                     LGLKSK"
     gene            179..>828
                     /locus_tag="ACHW42_004499"
     CDS             179..>828
                     /locus_tag="ACHW42_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR8946002.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYP"
BASE COUNT          301 a          106 c          154 g          267 t
ORIGIN      
        1 tgttttaaaa gcaaaagatc gcattgatct tgaacggcac tataatataa aaaatgaatt
       61 gaaagacttt ggctatacat atcttatatt caaaaagaaa attaaaaaag taatcaggga
      121 tgtaaaagtc aaactaggat tgaagagcaa ataatgatta tgatgaaggg taattaaatt
      181 ggattttaaa catcttactc aatttaaaga tataattgaa ctggacaagc gccccgttaa
      241 acttgatgaa cgggaaacgt ttaatgtctc atggggtatt gatgagaact accaggttgg
      301 ggctgcgatt tcaattgctt caattcttga aaagaataaa caaaacaaat ttacctttca
      361 cataatcgct gattacttag acaaagagta tattgaatta ttatcacaat tagcaacgaa
      421 gtatcaaaca gtaattaaat tatatcatat tgattctgag ccattgaagg cgctacctca
      481 atcaaatatc tggccagtat ctatttatta tcgtttgctt tcatttgatt atttttctgc
      541 gcgattggat tcattattat atcttgatgc tgatatcgtc tgtaagggtt cattgaacga
      601 gttaatagca ttagagttta aagatgaata tggggcagtg gtaattgatg tagatgctat
      661 gcaaagtaaa agcgctgagc gtttgtgtaa tgaggatttt aacggtagct attttaactc
      721 tggtgtaatg tatattaatt tacgggaatg gttaaaacaa agactaacgg aaaaattctt
      781 tgatctatta tcagatgagt caattataaa aaaattaaag tacccgga
//
LOCUS       DAZSNM010000483          821 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZSNM010000483 DAZSNM010000000
VERSION     DAZSNM010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.493"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(121..726)
                     /locus_tag="ACHW42_004500"
     CDS             complement(121..726)
                     /locus_tag="ACHW42_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8946003.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          235 a          187 c          134 g          265 t
ORIGIN      
        1 ttattattta aattcaaacc accagcttca tctgcgtgtc accggtatcc catatttttg
       61 ggatgtggct ataattatct attcccgcta tttagtcttc aaaaaaaatt acatttaata
      121 tcatttatct tgattaagaa atagtaagac taaaaaaaca agcgtcgtca gccagcacac
      181 tgctaacacg gtaagaacgc taataaaaac actccactgc ttcgttctat accattcaat
      241 atttttgtca gtatgtttta ttgggagcag ctcttcagta gagaattcct ggctgttatc
      301 attctcagga atgtttgcat cagtgtttac ttccctggcc tcgctatcgt tggtatagct
      361 actactgtct tcatcactgt cacgctcatt ctcacagaca tgtttctcga cctttcccag
      421 atatctgacg ccttttcgcg gaatggtttt aattagagaa ctcgacaaac ctgcgacgtc
      481 taatgccttt ctaagcatgt aaatctgccc ataataactg ctgtcgctta ccgaaccttt
      541 ctgctcccgc caaacctcat tgataatatg ttgcttatcg gattctccct gtagtaaaag
      601 gagtaaaaat cgatagccgt tttcagacag attgattaca ttgcctgtac tggtggtgat
      661 acgtcgagac gctgggtaaa acttaactcc ctgccctaaa gaaaaataaa aacggtcatc
      721 gctcataagt accgacccct gttttcttct tcacatgact actcattaca actttgtatg
      781 aagtaagatg caaaatcata ttgtttacat taagttaagc a
//
LOCUS       DAZSNM010000484          806 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZSNM010000484 DAZSNM010000000
VERSION     DAZSNM010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.494"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>806
                     /gene="ipaH1.4"
                     /locus_tag="ACHW42_004501"
     CDS             <1..>806
                     /gene="ipaH1.4"
                     /locus_tag="ACHW42_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH1.4"
                     /protein_id="HFR8946004.1"
                     /translation="SGIMHQINNVYSLTPLSLPMELTPSCNEFYLKTWSEWEKNGTPG
                     EQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNLLTHLPDLPPML
                     KVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHNNRLHILPLLPP
                     ELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVLMNNNLTTLPES
                     VLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPQIFFSMGNSATISA"
BASE COUNT          271 a          174 c          134 g          227 t
ORIGIN      
        1 tctggcatta tgcatcaaat aaacaatgta tactcgttaa ctccattatc tttacctatg
       61 gaactgactc catcttgtaa tgaattttat ttaaaaacct ggagcgaatg ggaaaagaac
      121 ggtaccccag gcgagcaacg caatatcgcc ttcaataggc tgaaaatatg tttacaaaat
      181 caagaggcag aattaaattt atctgagtta gatttaaaaa cattaccaga tttaccgcct
      241 cagataacaa cactggaaat aagaaaaaac ctattaacac atctccctga tttaccacca
      301 atgcttaagg taatacatgc tcaatttaat caactggaaa gcttacctgc cttacccgag
      361 acgttagaag agcttaatgc gggtgataac aagataaaag aattaccatt tcttcctgaa
      421 aatctaactc atttacgggt tcataataac cgattgcata ttctgccact attgccaccg
      481 gaactaaaat tactggtagt ttctggaaac agattagaca gcattccccc ctttccagat
      541 aagcttgaag ggctggctct ggctaataat tttatagaac aactaccgga attacctttt
      601 agtatgaaca gggctgtgct aatgaataat aatctgacaa cacttccgga aagtgtcctg
      661 agattagctc agaatgcctt cgtaaatgtt gcaggtaatc cattgtctgg ccataccatg
      721 cgtacactac aacaaataac caccggacca gattattctg gtcctcaaat atttttctct
      781 atgggaaatt ctgccacaat ttccgc
//
LOCUS       DAZSNM010000485          805 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZSNM010000485 DAZSNM010000000
VERSION     DAZSNM010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.495"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            42..758
                     /locus_tag="ACHW42_004502"
     CDS             42..758
                     /locus_tag="ACHW42_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR8946005.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          266 a          136 c          171 g          232 t
ORIGIN      
        1 tttaccagta atgtaaataa attttcgagg agatcattcc agtgggacgt aaatgggcca
       61 atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc taaaatttat gcaaaattcg
      121 gtgtagaaat ctatgctgct gccaaacaag gtgaacccga tccagaatta aacacatctt
      181 taaaattcgt tattgaacgt gcaaagcagg cacaagttcc aaagcacgtt attgataaag
      241 caattgataa agccaaaggc ggcggagatg aaacgttcgt acagggacgt tatgaaggct
      301 ttggtcctaa tggctcaatg attattgctg agacattgac ttcaaatgtt aaccgtacga
      361 ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa tatcggagcg gcaggttctg
      421 tcagctatat gtttgacaat acgggtgtga ttgtatttaa aggaacagac cctgaccata
      481 tttttgaaat tttgcttgaa gctgaagttg atgttcgtga tgtaactgaa gaagaaggta
      541 atattgttat ttatactgaa cctgctgacc ttcataaagg aatcgcggcg ctaaaagcag
      601 ctggaatcac tgagttctca acaacagaat tagaaatgat tgctcaatct gaagttgagc
      661 tttccccaga agatttagaa atctttgaag ggcttgttga tgcccttgaa gatgacgacg
      721 atgtacaaaa agtttatcat aacgtcgcaa atctctaatt atattttaaa gaaatctgtc
      781 tttatggcag atttctttaa tctca
//
LOCUS       DAZSNM010000486          804 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZSNM010000486 DAZSNM010000000
VERSION     DAZSNM010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.496"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..139)
                     /gene="ospE1"
                     /locus_tag="ACHW42_004503"
     CDS             complement(<1..139)
                     /gene="ospE1"
                     /locus_tag="ACHW42_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HFR8946006.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IY"
     gene            complement(248..378)
                     /locus_tag="ACHW42_004504"
                     /pseudo
     CDS             complement(248..378)
                     /locus_tag="ACHW42_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611212.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            376..459
                     /gene="tnpB"
                     /locus_tag="ACHW42_004505"
                     /pseudo
     CDS             376..459
                     /gene="tnpB"
                     /locus_tag="ACHW42_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          237 a          137 c          187 g          243 t
ORIGIN      
        1 cgtatatagc tgctttttta ttgaaaacag tatatccatc tgtagtaaca gtagaagata
       61 ataatacagg atttgatact tcaaggatga tattttcttg tttttgtgga agacaaggga
      121 atatagtttg tgtaagcata ttacttcctt atgttatatt ttggtaaaaa atttattttt
      181 ttacattata gtcggctaaa tgaagtttac tgtaaaaaaa cgatactttt tcgggtggcg
      241 cttccaatca gtagaatatg tgtttttcaa tgaaggagcc gatgactttt cgtggatttc
      301 cacggaacgc gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt
      361 caggttgtta cgttcaagat atagcatgtg ctggatgaag atcccttttc tggtcacctg
      421 tttatctttc gtggccgacg gggtgacact gttaaaatgt aatgaacgca tcccacacaa
      481 ttaaacacgg agtagtatga tggttaaaag ggtgaaactg gcaggtgtgg agcctcgcag
      541 ctttccggca tcatactgcc caatacggga tgcataaagc gtcctcagac tggagactcc
      601 acaaatgaaa tatacaccgg ttggcgttga tatggcaaaa catgtcattc agattcactt
      661 catcaatgag cacatgtcag cgccagtgat ataagacggt aatggggatg cggacgattt
      721 cctggactct cagaagatga aacatgttta ctgaagaaca aaaaattcgc gctattgagc
      781 tttatttcaa atatggtaag aagc
//
LOCUS       DAZSNM010000487          795 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZSNM010000487 DAZSNM010000000
VERSION     DAZSNM010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.497"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(217..292)
                     /locus_tag="ACHW42_004506"
     tRNA            complement(217..292)
                     /locus_tag="ACHW42_004506"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(257..259),aa:Ile,seq:cat)
BASE COUNT          247 a          176 c          122 g          250 t
ORIGIN      
        1 tttgcccgct tggcatagcg ggcatttttt ccaggtactt ttgaatgagt actgatgaat
       61 aaatacattg cagtggcgtg ccacgtacca aaacaccagc cctcattcga aaccacccac
      121 cgcacttctt ccttgaaatg gcgttagtca tgaaatatag accgccatcg agtacccctt
      181 gtacccttaa ctcttcctga tacatgaatg atgatttggt ggcccttgct ggacttgaac
      241 cagcgaccaa gcgattatga gtcgcctgct ctaaccactg agctaaaggg ccttaagtat
      301 gcaataacaa tacttataaa ccacgcaata aacatgatga tcttatgatg taaataacag
      361 atttttatgc gttcccattc accttgggtc gtgattacac gcgacataaa acccgacacc
      421 gcctccattc gcaaagtcga tactcgcagt caacaagcaa atgttaataa ttagcactat
      481 ctatagttat catcgattca atgatacttt gtaatgattt tgtatctaat aatataactt
      541 tattacatta gctgaagagt tttcgcatca ttatgatatc tgttactttt cactccataa
      601 aaataaactt catatagcaa tatattcttt catagatctt attctgctaa tcattagttt
      661 cgtatgcgcg atttttgaca gttgcctctc cagaccacat cgataattaa aaaacagatt
      721 taaacattat ccttttccat atagatattg gataaaaata ggacatctgt ttgcaattac
      781 tttcacaaca attaa
//
LOCUS       DAZSNM010000488          793 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZSNM010000488 DAZSNM010000000
VERSION     DAZSNM010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.498"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            36..793
                     /locus_tag="ACHW42_004507"
                     /pseudo
     CDS             36..793
                     /locus_tag="ACHW42_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          232 a          164 c          197 g          200 t
ORIGIN      
        1 attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa tacccttttg
       61 aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac aggatcatct
      121 cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc tatgaaaaac
      181 atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca gagttgcgta
      241 ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct gctgctcact
      301 ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggtttatgaa gagcgcggag
      361 aagctggttt acgcgcgctc aagattggca ccaaaagaaa cattgcaata tcagttgatc
      421 cagaaaaagc ggcatcagca ttggagctgt caaaagaccg acgcattgag gatcttgaaa
      481 ggcaagttcg atttcttgaa acgcggcttg tgtatctaaa aaagctgaaa gccttagctc
      541 atcccacgaa aaagtgaaag tactcaacga gctaaggcag ttttatcctc ttgatgagct
      601 tctcagggct gcggagatac cgcgcagtac gttttattac catctaaagg ctctcagcaa
      661 gcctgacaag tatgcggacg ttaaaaagcg tattggtgag atttatcatg agaatagagg
      721 ccgatacgga taccgtaggg taacgctgtc tcttcatcga gaagggaaac agattaacca
      781 taaagctgtt cac
//
LOCUS       DAZSNM010000489          788 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZSNM010000489 DAZSNM010000000
VERSION     DAZSNM010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.499"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..144)
                     /locus_tag="ACHW42_004508"
     CDS             complement(<1..144)
                     /locus_tag="ACHW42_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR8946007.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVI"
     gene            complement(170..>788)
                     /locus_tag="ACHW42_004509"
     CDS             complement(170..>788)
                     /locus_tag="ACHW42_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR8946008.1"
                     /translation="DIACKGSIKELLDYQFSTNEIAAVVAERDIEWWQNRASVLTTPQ
                     LASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKF
                     ISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAAS
                     PWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKCF"
BASE COUNT          258 a          143 c          107 g          280 t
ORIGIN      
        1 aatgacttta gtatcatcaa tagaacgaaa aacttttaat gttttaaccc tgcaagttaa
       61 aaagtcattt agaacttgct cataaaaggg atcattatct tttatgaaaa ctttcaaccc
      121 atttatcgtt ttattatata tcataatgaa ttattttaac cttaaatctt tagaagcatt
      181 tttctttata atactttaaa taattaaaaa tgcctgccat ataatgcttt tgtttaaatt
      241 tatgttttgc acaataccga tactgattgc tatttacagg tttaagtaaa tcttctttac
      301 tccacggaga agctgctttg gcgatcaaaa aactacgtga aaccggataa tcagcccact
      361 catgccaggg ttttgtaggt cccacatagt gtataaacac ggtgtcatca ttgactggat
      421 tatcaacttt gtcttttaat tcatagttaa tactatatcg ggtattatat ttctccgaaa
      481 taaacttcac tttaccattc aataatacat tcagtacatc ttgatcaagg tgggtgattt
      541 tacttaccca atctgggtca cgcaacattt caatagcttt tgacgaaatg ttatttagat
      601 tccactcatc aatatttatc agtaaaaaac cagcattgaa atatccagaa gctaactgtg
      661 gtgtagttaa taccgaggct cgattttgcc accattcaat atctctttca gctacaactg
      721 cggcaatttc attagtagaa aattgataat ctaagagttc tttaatacta cccttgcaag
      781 caatatct
//
LOCUS       DAZSNM010000490          784 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZSNM010000490 DAZSNM010000000
VERSION     DAZSNM010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.500"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..605)
                     /locus_tag="ACHW42_004510"
     CDS             complement(<1..605)
                     /locus_tag="ACHW42_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HFR8946009.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPD"
     gene            complement(635..>784)
                     /locus_tag="ACHW42_004511"
     CDS             complement(635..>784)
                     /locus_tag="ACHW42_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HFR8946010.1"
                     /translation="ELVPYLALLYFMVEKYGLLGAAISWSIRVILDALLLQWLYTRMC
                     SVYEN"
BASE COUNT          260 a          149 c          107 g          268 t
ORIGIN      
        1 atatcaggaa tgatatgaac taatatgcca tagtttaagc atctaagaca ccattctgta
       61 tcgacataat caataaaaag ttcctcatcc atcattccaa caatctcaaa aacttctttt
      121 gaaaccatgg ttccggatga gattgttact gaacttttaa acggtgtctg tccttcagtg
      181 acatgaattt tctctcgaag accatttttt ttgatattac aaataggata ataatgtgac
      241 ttatctctat caataaaaac tgggccagct atttttgcat tttcattatc catatagcag
      301 gaaagttttt tgataaactc attgcgaata cggctatctt gatcaaaaaa aattatcttc
      361 tctgcccctt tttcaagaga atatcgaatc ccaatatttt gtgcttttgc tataccttca
      421 ttcctaccaa gacaaattaa atgaactttt tcaatgtctg ctaatttatc tttcaaggtg
      481 aaattagtcc tattatcgac tactacaacg taacaatttt gctcctttag ttcagtgatc
      541 agtgcattca gatttttaag ttcgggatta tatgtcacaa tgacagcgta aatattacta
      601 ttcattatcc aagtgactca gtaattggtt aattttagtt ttcatataca gaacacattc
      661 tagtataaag ccattgtaat aacagagcat ctaaaattac acgtatgctc caggatattg
      721 cggcacccag taacccatat ttttcgacca taaaatacaa aagggctaaa taaggaacca
      781 attc
//
LOCUS       DAZSNM010000491          781 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZSNM010000491 DAZSNM010000000
VERSION     DAZSNM010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.501"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            187..480
                     /gene="yicS"
                     /locus_tag="ACHW42_004512"
     CDS             187..480
                     /gene="yicS"
                     /locus_tag="ACHW42_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR8946011.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..>781)
                     /locus_tag="ACHW42_004513"
     CDS             complement(521..>781)
                     /locus_tag="ACHW42_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8946012.1"
                     /translation="VAIIWGLTFALVPVGWSTWITRSLADQAEKAGSIQVAVIQLANT
                     CGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVKMKKS"
BASE COUNT          251 a          184 c          168 g          178 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 c
//
LOCUS       DAZSNM010000492          779 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZSNM010000492 DAZSNM010000000
VERSION     DAZSNM010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.502"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..663)
                     /locus_tag="ACHW42_004514"
     CDS             complement(<1..663)
                     /locus_tag="ACHW42_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFR8946013.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IAL"
     gene            complement(653..>779)
                     /locus_tag="ACHW42_004515"
     CDS             complement(653..>779)
                     /locus_tag="ACHW42_004515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HFR8946014.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          251 a          203 c          175 g          150 t
ORIGIN      
        1 cagtgctatt ttgattcgac gttgatcaag tttctgataa ttccaacgac ggtcgacggg
       61 caccatccag tcaatcaaca tacctaccgc cagccctgtg atgacatcaa taaaatgatg
      121 ctgccatgtc gtcagcgtcg aaatggcgat gagtaaaaac catccgccac agactttacg
      181 ccacctcaca gccagatgct gacgaaagtg acgccaaagt agccagcaga gaataatatg
      241 cagcgaagga gactggttat aaggcagatc aaacagttca agttgcgaaa atagccatcc
      301 tgtcacccca ctcacttcag gacggataaa actaaacttc agcgggtaga gaaaaaaacc
      361 gcagcaggcc attaccgttg ccagaataag ccggtggaca agtcggcgtt gttcgaatgt
      421 cgagctacaa acgaacagcg aaaatccata taaaagatcc agactccagt aaggaacaat
      481 agtccaggga agaaaaggga tcgccgtttc ccaaccgaat acctgactgg ggatatcatg
      541 gctgttaagg tcctgaaccg cggtgaactg attaagagat ccataggtga agaagaaaaa
      601 cggggccagc aacaataacc agccagcgcc ttgtagcaat acgttccgac gttcagttat
      661 cacgacgcac cgccatcgaa accgtaaaaa tgccccacac atcaatccgt tgagtgcatt
      721 tatcaaatcc ggcattacgc acgagcgaat ccatctcccc ttgcgaacgc acgcgcatt
//
LOCUS       DAZSNM010000493          770 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZSNM010000493 DAZSNM010000000
VERSION     DAZSNM010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.503"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="ACHW42_004516"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="ACHW42_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR8946015.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT          201 a          144 c          164 g          261 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc
//
LOCUS       DAZSNM010000494          763 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZSNM010000494 DAZSNM010000000
VERSION     DAZSNM010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.504"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..698
                     /locus_tag="ACHW42_004517"
     CDS             <1..698
                     /locus_tag="ACHW42_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR8946016.1"
                     /translation="SVTDLAKATGVSEVTIRQDLNTLEKLSYLRRAHGFAVSLDSDDV
                     ETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLARTLGEQKKNVTIITVSSY
                     IAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHFSKAFIGIDGWQPETGFTG
                     RDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVERFNRVITDSKIRASDLMH
                     LEHSKLTVHVVDI"
BASE COUNT          195 a          192 c          186 g          190 t
ORIGIN      
        1 ttagcgtaac cgatctggca aaagccactg gagtttctga agttaccatt cgccaggatc
       61 tcaacaccct cgaaaaactg agttacctcc gccgtgcaca tggctttgca gtttcgcttg
      121 atagtgatga cgtcgaaacc cgtatgatga gcaactatac gctgaagcgt gaactcgccg
      181 agtttgccgc gtcactggtt caaccgggcg aaaccatctt tatcgaaaat ggcagcagca
      241 atgccctgct cgcccggact ctgggggagc agaagaaaaa tgtcactatc atcacggtca
      301 gcagctacat cgcgcatttg ctgaaagacg cgccttgtga agtcattttg ctcggtggcg
      361 tgtaccagaa aaaaagcgaa agtatggttg gccctttgac acgccagtgc atccagcagg
      421 tacatttcag caaagcattt attggtattg atggctggca acctgaaact ggattcaccg
      481 gtcgcgatat gatgcgcacc gatgtggtca atgccgtgct ggaaaaagag tgcgaagcga
      541 tagtcctgac tgacagctcg aaatttggtg ctgtacattc atactccatc ggtcccgttg
      601 agcgattcaa tcgcgtgatt accgattcga aaatacgcgc cagcgatctg atgcatcttg
      661 agcacagtaa actcaccgtt cacgtcgttg acatttaacc atttcccttc tctacggatg
      721 atttgcagtt tggcaaatca tccgctctaa gatgattcct ggt
//
LOCUS       DAZSNM010000495          761 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZSNM010000495 DAZSNM010000000
VERSION     DAZSNM010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.505"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..648
                     /gene="ospC2"
                     /locus_tag="ACHW42_004518"
     CDS             <1..648
                     /gene="ospC2"
                     /locus_tag="ACHW42_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFR8946017.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNAKSDMALYLLSKFNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYK
                     VLEYFISNGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          278 a           98 c          150 g          235 t
ORIGIN      
        1 aaaactgatg tgccaatatt taatactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa agattccgag agttttgtta taataaaaac
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagcacaga taacttcaaa aagataagac tcagagatat atctttggag
      241 gatgccatta aagcatccaa ttatgaagaa attaacaata aggtgactga taaaaaaatg
      301 gctcatcaag ctcttgctta ttctctaggc aatgcaaaat ctgatatggc actttattta
      361 ctatctaaat ttaattttac aaaacaagat atcgcagaga tggagaaaat gaacaacaat
      421 atgtattgtg agctgtatga tgttgagtat ttacttagtg aagatagtgc caactataaa
      481 gtgctagaat attttattag taatggattg gttgatgtaa acaaaagatt tcaaaaagca
      541 aatagtgggg acactatgct tgacaatgca atgaaaagca aagattcaaa aacaattgat
      601 tttttattaa aaaatggagc ggtatcaggc aaacgatttg ggaggtgacc taaaatatat
      661 ttaggttgtg ccctgaatct gacgttgtca tttttgttat attgatcgat ataggggatg
      721 gtgcagaccg ttcccctgca acaacacatt gcatgtggtg a
//
LOCUS       DAZSNM010000496          759 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZSNM010000496 DAZSNM010000000
VERSION     DAZSNM010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.506"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>759
                     /locus_tag="ACHW42_004519"
     CDS             <1..>759
                     /locus_tag="ACHW42_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR8946018.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          209 t
ORIGIN      
        1 gagcgcattc tgaccgaccg tgaaaatggt gaaaaaggcg ataattctca agatacctct
       61 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
      121 cagtttgaac tagacctgga agcatcaaac agacacaacc ctgaccaaac tcgtttggca
      181 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      241 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcggtga ctcatgggct
      301 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      361 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      421 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      481 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      541 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      601 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      661 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      721 tccatcggtt actcctattc taaagcactg gacgtagca
//
LOCUS       DAZSNM010000497          758 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZSNM010000497 DAZSNM010000000
VERSION     DAZSNM010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.507"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..351
                     /locus_tag="ACHW42_004520"
     CDS             <1..351
                     /locus_tag="ACHW42_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8946019.1"
                     /translation="QPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQENK
                     VIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLENVNI
                     THWAVMPKAPEAGA"
     gene            351..656
                     /gene="yfdT"
                     /locus_tag="ACHW42_004521"
     CDS             351..656
                     /gene="yfdT"
                     /locus_tag="ACHW42_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFR8946020.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            572..>758
                     /locus_tag="ACHW42_004522"
     CDS             572..>758
                     /locus_tag="ACHW42_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8946021.1"
                     /translation="MGYDSRLDRLAATSWYPFLNNVTARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQAT"
BASE COUNT          241 a          163 c          189 g          165 t
ORIGIN      
        1 cagcctgagt tcacagataa aacactctcc aggaaacccg gggcggttca gaaagtaaat
       61 gaaacagatg ctgaatacct ggtgcgtaaa ttcgatgagc ttgaagctaa atgtgcagca
      121 caggaaaaca aagtaatacc agtgtcaact gaactgccac cagcaaatga aagtgttttg
      181 ttattcgatg ctaacggaga aggctggcta attggctggc gttctctctg gtacacctgg
      241 ggacaaaaag aaaccggaga atggcagtgg acatttcagg tcggggacct tgaaaacgtc
      301 aatatcactc actgggcagt aatgccaaaa gcaccggagg ctggagcata atgaccactt
      361 ttaccgacaa agaactgatt aaagaaatta aagagcgtat cagcagcctt gacgtgcgag
      421 acgatattga gcgccgtgct tatgaaatcg cactcctatc tctggaagta gaaccagatg
      481 aacgcgaagc ttatgaatta ttcatggaaa agcgttttgg tgacttagta gatcgtcgga
      541 gagcaaaaaa cggcgataac gaatacatgg catgggatat gactctcggt tggatcgtct
      601 ggcagcaacg agctggtatc catttctcaa caatgtcaca gcaagaggtg aaataatgga
      661 gccatacagc ctcacactcg atgaggcctg tcattttctc aagatatcca gaccgactgc
      721 cattaactgg atacgcacag ggcgtcttca ggcaacac
//
LOCUS       DAZSNM010000498          756 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZSNM010000498 DAZSNM010000000
VERSION     DAZSNM010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.508"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..61)
                     /locus_tag="ACHW42_004523"
     CDS             complement(<1..61)
                     /locus_tag="ACHW42_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HFR8946022.1"
                     /translation="MRKLYAAILSAAICLAVSGA"
     gene            complement(129..>756)
                     /locus_tag="ACHW42_004524"
     CDS             complement(129..>756)
                     /locus_tag="ACHW42_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host specificity protein"
                     /protein_id="HFR8946023.1"
                     /translation="FMNDVFLKRLTAPTITSGGNPPAFSLTPGGRLTAKNADISGNVN
                     ANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVGKPFPRDSRAPERWPSGTITVRVY
                     DDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLIVKKNGAEIYNRTTLDNTLIYTGV
                     IDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLVVVMKKSTAGISIS"
BASE COUNT          186 a          199 c          177 g          194 t
ORIGIN      
        1 gcgcaccgga tacagccaga cagatggctg cggacaaaat ggcagcataa agtttacgca
       61 taattacctc tcgcttttct gcaataaaaa aggcaccatt tctggtgccc gtatctgggt
      121 tataaaattc agctgatact gatacctgct gtggatttct tcatcacaac aaccagcaaa
      181 tcgctgatac ttgctgtggg ataccagcca tttaccagcc atgccgatac agaaaactcc
      241 agcgtcatgt gaccgtgacc ggcaggcata tcaataacac ccgtatatat cagcgtatta
      301 tccagggtcg ttcggttata aatttcagca ccgtttttct tcactatcag gcggcatgac
      361 gaataaatat tgttattctt ccgctcatgt ttagcaccgc gaaacgccac cgcgggaata
      421 acaatttgcc gatcaaacgg ctgatcgtca taaaccctga cggtaatggt ccctgatggc
      481 cacctctccg gtgcccggga gtcccgtggg aaaggtttgc ccactgtttt aacgagatcg
      541 ccttcaatct ggttcgcgga caattttccc agaacccgac agttcttatt aatcgtgacg
      601 ttgttgagcg tcccggagtt cgcgttcacg ttaccgctga tatcggcatt tttcgccgtc
      661 agccgtccgc caggtgtcag ggaaaatgcc ggaggattac cgccgctggt aatggtcgga
      721 gccgtcaggc gtttcaggaa cacgtcgttc atgaat
//
LOCUS       DAZSNM010000499          752 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZSNM010000499 DAZSNM010000000
VERSION     DAZSNM010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.509"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>752
                     /locus_tag="ACHW42_004525"
     CDS             <1..>752
                     /locus_tag="ACHW42_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8946024.1"
                     /translation="KKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQSLQRLRAQ
                     FNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFIIYGPQLIS
                     CSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVISRSVICMP
                     LHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEKFMNRKIS
                     FRGSSLLTIINSIAVTDLLGIVPYELYNYHRD"
BASE COUNT          237 a          167 c          137 g          211 t
ORIGIN      
        1 tgaaaaagtt cgacttaaat cttcttgtca tatttgagtg tatttaccag catttaagca
       61 ttagcaaagc tgctgaatca ctatatatca ccccatcagc cgtgagccag tcgttacaac
      121 gcttacgtgc gcaatttaat gaccctttat tcattcgctc aggaaaaggt atcgcgccga
      181 cgacaacagg gttgaatctg catcaccatc ttgagaaaaa cctcaaaggc cttgaacaaa
      241 caattaatat agtcaataag tccgaactaa agaaaaactt tattatctac ggccctcagc
      301 tcatttcttg ctctaataat agtatgctga tccgctgcct acggcaagat gcctctgtcg
      361 agattgagtg ccatgatatc ctcatgtcag cagagaatgc tgaagaactg ttggttcacc
      421 gcaaagccga tctggtgatc acccaaatgc cagtgatcag ccgttccgtc atttgcatgc
      481 cattgcatac aattcgcaat actctaattt gtagtaataa gcatccgcga attactgaca
      541 atagcactta tgaacaaatt atggcagaag agttcaccca attaatagcc aaatcagcag
      601 gagttgatga tattcaaatg gaaatagacg agaagtttat gaatcgcaaa atttcatttc
      661 gtggttcctc tttgctgaca ataattaata gcattgcagt tacagattta ctgggaatag
      721 tcccctatga actttataac taccaccgtg ac
//
LOCUS       DAZSNM010000500          751 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZSNM010000500 DAZSNM010000000
VERSION     DAZSNM010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.510"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            43..>751
                     /gene="mxiJ"
                     /locus_tag="ACHW42_004526"
     CDS             43..>751
                     /gene="mxiJ"
                     /locus_tag="ACHW42_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFR8946025.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWF"
BASE COUNT          244 a          104 c          152 g          251 t
ORIGIN      
        1 aacagtttcg gctgttgaaa ctttattaaa gtcttaattt atatgattag gtataaaggt
       61 tttattttat tcttgttgct gatgttgatt ggatgtgagc aacgtgaaga gttaatttct
      121 aatttatctc aaagacaggc aaatgaaata atatctgtgc tagaacgcca taatattact
      181 gctagaaaag ttgatggagg taaacagggg atctcggtac aagtcgaaaa ggggacattt
      241 gcatcggcag ttgatttgat gcgcatgtac gatttgccaa atccggagag agttgatatc
      301 tcacaaatgt ttcctacaga ttcattagtg tcttctccaa gagctgaaaa ggcccgttta
      361 tatagtgcta ttgagcaacg gctggaacag tctttagttt ctattggtgg tgttatttcg
      421 gcaaaaatac atgttagcta tgatcttgaa gaaaaaaata tatcttcaaa accgatgcat
      481 atatcagtaa tcgctatata tgactcaccg aaagagtctg aactattagt tagtaatatt
      541 aagcgatttt tgaaaaacac cttttctgat gttaagtatg aaaatatatc tgtcatatta
      601 actccgaaag aagaatatgt ttatacaaat gtacaacctg ttaaggaagt taaatcggaa
      661 tttttaacaa atgaagtaat atatttattt ctcgggatgg ctgtactagt tgtcattctt
      721 ttggtatggg cattcaaaac agggtggttc a
//
LOCUS       DAZSNM010000501          748 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZSNM010000501 DAZSNM010000000
VERSION     DAZSNM010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.511"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            6..224
                     /locus_tag="ACHW42_004527"
     CDS             6..224
                     /locus_tag="ACHW42_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR8946026.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="ACHW42_004528"
     CDS             226..591
                     /locus_tag="ACHW42_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR8946027.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
BASE COUNT          221 a          143 c          190 g          194 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatggggg
//
LOCUS       DAZSNM010000502          745 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZSNM010000502 DAZSNM010000000
VERSION     DAZSNM010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.512"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..408
                     /locus_tag="ACHW42_004529"
     CDS             <1..408
                     /locus_tag="ACHW42_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFR8946028.1"
                     /translation="EMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHT
                     KFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVV
                     TLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            591..>745
                     /gene="bfd"
                     /locus_tag="ACHW42_004530"
     CDS             591..>745
                     /gene="bfd"
                     /locus_tag="ACHW42_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFR8946029.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQ"
BASE COUNT          185 a          169 c          194 g          197 t
ORIGIN      
        1 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
       61 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      121 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggccgt
      181 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      241 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      301 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      361 ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc tgggctaatt acacgttaat
      421 tagttttgaa ttgaaaaggg cgcttcggcg ccctttttgc tgcccgtaat ttgagctcga
      481 aatatttagt cgtaattttg ctcgctgaac tatggcgcaa ataaaactgt gctattgtaa
      541 tcataaccat tctcatttac actttgtgca gaatttgaac gggagcaatc atgtacgttt
      601 gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc agtccgtcag tttcatccgc
      661 aatctttcca acaattgcgt aagtttattc ctgtgggaaa tcaatgcggt aagtgcattc
      721 gcgccgcgcg cgaagtgatg cagga
//
LOCUS       DAZSNM010000503          722 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZSNM010000503 DAZSNM010000000
VERSION     DAZSNM010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.513"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..636
                     /locus_tag="ACHW42_004531"
     CDS             <1..636
                     /locus_tag="ACHW42_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR8946030.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT          230 a          147 c          181 g          164 t
ORIGIN      
        1 ccgttatcta aaacagaatt caatgcatta atggaaagtg aaaaaggaca gagccaaaac
       61 ttgtggaaat ttgccgttta ctccgggctt cgtcacgggg aactggcagc tctggcgtgg
      121 gaggatgtgg atttcgagaa gggaattgtg aatgtcagaa gaaacctgac gatacttgat
      181 atgttcggtc ccccaaaaac aaatgccggg atccggacgg tagcattact gcagccggct
      241 cttgaagcac tgaaggagca atacaaactg accgggcatc atcgcaaaag cgaaatcact
      301 ttttatcatc gggagtacgg cagaactgaa aaacaaaaac tgcattttgt tttcatgccc
      361 agggtgtgta acgaaaaaca gaaaccttat tactcggtaa gcagtttggg tacaagatgg
      421 aatgcagcag taaaacgtgc tggtattcgc cgccgtaatc cgtaccatac gcgacatact
      481 tttgcctgct ggctgttgac ggcaggagcg aacccggcat ttatagccag ccagatgggg
      541 catgaaactg cgcaaatggt gtatgaaatt tacggtatgt ggattgatga catgaacgac
      601 gaacagatag ccatgttgaa tgcgcggtta tcgtagttgc aaagtttgcc cccaatttgc
      661 cccatttagt accagagaac tgaaataatg caagaaaatc aacaaattac aaagaaagaa
      721 ca
//
LOCUS       DAZSNM010000504          721 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZSNM010000504 DAZSNM010000000
VERSION     DAZSNM010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.514"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            2..721
                     /locus_tag="ACHW42_004532"
                     /pseudo
     CDS             2..721
                     /locus_tag="ACHW42_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          229 a          112 c          140 g          240 t
ORIGIN      
        1 gatgttttct acaaacttaa tacagagtaa ttatggtgat ttaaatatta aaagcctggc
       61 tttcgactct tttaaagaaa ggcttgagtc taccatggcg gcactcactt tctttatatc
      121 aacgggtcaa tgtgactgcg atgaaatagc agagtcgaac ttcaatcgta tgattgccta
      181 tatgtctaac attaactatg atgcctcaaa gctaggagcg ccagcactta gttttgatac
      241 gtatctgcag gacaatgtta aatatagagt gataattaat aatctttatg gcagtgagtt
      301 tcggataaga gatattaatg agaattttgt tgggatggat tttacctgtg aattccgacc
      361 cgaaaaaatg acgaatttga tcaatataaa aaataggttg gttattcatt atttaaggac
      421 tatatactat gaaaaaaatt atatacatcc tgccgggagc atttttgctg cgattcaaaa
      481 aaaatgagag tctcctaaag tttccttcga taatatcaat gcttaatatc aatcttcttt
      541 ttaatccact aaatcttccg ggaatgggaa gtggtgtgct tgaagatatt atgagtattc
      601 cagatagttc acttagaaaa cgtctcgggt atgaggtttt aagcttctca ctacaggcac
      661 attcattgag ccaggaatgt attgataagt tggacatttt tttcgctgac gatctgttta
      721 a
//
LOCUS       DAZSNM010000505          719 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZSNM010000505 DAZSNM010000000
VERSION     DAZSNM010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.515"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..433)
                     /locus_tag="ACHW42_004533"
     CDS             complement(<1..433)
                     /locus_tag="ACHW42_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8946031.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYI"
     gene            complement(426..>719)
                     /locus_tag="ACHW42_004534"
     CDS             complement(426..>719)
                     /locus_tag="ACHW42_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8946032.1"
                     /translation="FKDTYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEI
                     SKKHLPCVPGANFIYDVNSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
BASE COUNT          234 a          142 c          113 g          230 t
ORIGIN      
        1 aaatatagac attattaaac ttgcttagcc tcgcattgcc tttacctgtt aaagatgcat
       61 cgagaaaatt gagtctgaag ttctcatcct ttatctgatt ttccagtccc gaaactttgg
      121 ccttagcatt acctgagatc cgtccgttac tatctatttt gtaggtaaaa aaatacgaat
      181 gggaaaattt cctcggtgta tcttcatcag ttaagccaac aatcgtaaaa ctaccgctac
      241 catctttttt taaaatgact gaaaggttag cagagtagta taaagaatga tctgttttag
      301 catttcttgt taacgtcgac gtacattcaa gagacgttgt gctaccatca cgagccttcc
      361 agagagcaaa aaaacacacc tgtagcaatg tgaaaacaag tattaatata aaatacattt
      421 tcttattagt catataatag cacctctgaa caaattgcat aagtttcgtc attagaggat
      481 tttatacatc ttgataaata ccgctgccct tccccaagca aataatgcat actatttaca
      541 tcatatataa aatttgcacc tggcacacag ggtaaatgct ttttcgaaat ctcagcggcc
      601 tctatttgag atttagcgat gccaatctcg ttaagattct ctgaaagata taaatcacag
      661 gtgtcaatct tacctgattt aaccaatgga taatccgtta tactgtaggt atctttaaa
//
LOCUS       DAZSNM010000506          715 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZSNM010000506 DAZSNM010000000
VERSION     DAZSNM010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.516"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(219..398)
                     /locus_tag="ACHW42_004535"
                     /pseudo
     CDS             complement(219..398)
                     /locus_tag="ACHW42_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            492..>715
                     /locus_tag="ACHW42_004536"
     CDS             492..>715
                     /locus_tag="ACHW42_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8946033.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          191 a          141 c          190 g          193 t
ORIGIN      
        1 aatttataaa gaaatgcatt tggggtgttg ggttgaagag tagctggtag atgttattgt
       61 gccagcaggg gcaggggaat gtgccgtatc gatatgcttt ggcaattgcg tgtgccgcag
      121 atggagatga aatgccaata aaaaagcctg agttcgatga gggacgaact caggctggtg
      181 ttatgtttta cagtaacacg cctcggcgcg ttggctgatt agaactggta gacgatacca
      241 actgccagtt ggtcgtcggt gttcagtcca tgagcgagaa cataatcact cttatcaagc
      301 aggttgatct gataagcggt atatatgttc atattcttat tgaagtaata ccaggtaccg
      361 atttcaatat agttcagacg atcagaatct ttgtaacctg tagactggcc ccctgaatct
      421 ccagacaacc aatatcactt atttaagtga tagtcttaat actagttttt agactagtca
      481 ttggagagca gatgattgat gtcttaggac cggagaaacg cagacggcgt actacacagg
      541 aaaagatcgc tattgttcag cagagttttg aaccgggaat gacggtctcc cttgttgccc
      601 ggcaacacgg tgtggcagcc agccagttat ttctctggcg caagcaatac caggagggaa
      661 gtcttactgc tgtggccgca ggagaacagg tcgttcctgc ctctgaactt gctgc
//
LOCUS       DAZSNM010000507          714 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZSNM010000507 DAZSNM010000000
VERSION     DAZSNM010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.517"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          247 a           98 c          129 g          240 t
ORIGIN      
        1 taatgccaaa tattgttcgc tttggagagt attattgcct gtttgtgtct gttgagcaga
       61 aaaatcatct gaatttgaat acttttatta ggtgtgaata aatgaaaaaa tcattaaaat
      121 cattaaaatc attaaaatca ttaaaatcat taaaatcatt aaaatcatta aaatcattaa
      181 cttatatgaa tatttcagag ttggagttcg aatgttgaca cctgagtatt tcctgctaaa
      241 aatagccctg ctttacggat ggttgtattg caataacacg aaagtagtaa gagattaaag
      301 gtatctgtca gcgataactc tgcaagtgat gacattttgt aaaccaaata atgtaaatca
      361 gcaatgtatc ttttcactta attataacaa atgtggtatt cactaagaat ctgatacgag
      421 cttacatcac gactatttta gtctcatcgc gggatacagg gatgcaccga tggatctcag
      481 cacaccgtaa tgcaatacct gaacaaatag aacagtaaag tggttcagta ttaatgaaag
      541 ctcttatttt ccgttgggaa aacttaaaat agatgagaag gctttttgat ttcttatgtg
      601 atctcatttc gagtattgag aaatttgtga tttttaaatt tttcaataag ccctgtaagg
      661 gattggggtt ttatgagaaa taaagagacg tattgtcttt gtttaaacgg tacg
//
LOCUS       DAZSNM010000508          704 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZSNM010000508 DAZSNM010000000
VERSION     DAZSNM010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.518"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..443)
                     /locus_tag="ACHW42_004537"
     CDS             complement(<1..443)
                     /locus_tag="ACHW42_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR8946034.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          214 a          172 c          131 g          187 t
ORIGIN      
        1 tcatctggat aatcggcatg agcaataaca tgcttgccgg tgcgactgtg tacttcaaga
       61 ataaagggca aatgctgaca tttttccagc gccgttttcg cttgtttctg ttgattatct
      121 gccagcgcaa taaaccagtc gccgccattc atcaaccaca aagacatctg ctggaatgcc
      181 agcgcatcca tcgccatctg ttcatgattg cctcttaccg cacgaaccca atgttgttcc
      241 agtaactgca gacaacgtaa actttgcggc ccacgatcga taacgtctcc cactgagata
      301 agtaaatctc gccacggatc aaaacgacaa tgccataatt tgcggcgcaa ctgctcaaga
      361 caaccgtgta tatcgccaga aagccagata tgtcgccatt gatgacccgc aattctctga
      421 taaacgggcg caggctgttt catcaatatt ttcctcccgc gctaaagatc acataatctt
      481 aacaagaatg ttaaaaaacg ctggactcag acagtagagt gtgtgttatg gttgactata
      541 aagtcagcga aggaaatgct tctggctttt aacagataaa aagagaccga acatgattcc
      601 tgttttcgtc aacaaacaat aaaacccttt aaaattaatg cgttgaataa atttacgttc
      661 atcttttaat ccctgacacg taccattgca tattaataca ttca
//
LOCUS       DAZSNM010000509          700 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZSNM010000509 DAZSNM010000000
VERSION     DAZSNM010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.519"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..337
                     /locus_tag="ACHW42_004538"
     CDS             <1..337
                     /locus_tag="ACHW42_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR8946035.1"
                     /translation="LNIIDKESLCERANQLGQRLKNTLIDAKESVPAIAAVRGLGSMI
                     AAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDAQFDAAM
                     KILQDALSD"
     gene            complement(457..>700)
                     /locus_tag="ACHW42_004539"
     CDS             complement(457..>700)
                     /locus_tag="ACHW42_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8946036.1"
                     /translation="PLDDIIIDPFKRRPPEEAIAVSENTSLPTLPLDLREFQMQQEKE
                     LLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
BASE COUNT          173 a          180 c          182 g          165 t
ORIGIN      
        1 gctcaatatt atcgacaaag aatcactctg cgaacgcgcg aatcaactgg gccagcgtct
       61 caaaaacacg ttgattgatg ccaaagaaag cgttccggcc attgctgcgg tacgcggcct
      121 ggggtcgatg attgcggcag agtttaacga tccgcaaacg ggcgagccgt cagcggcgat
      181 tgcacagaaa atccagcaac gcgcgctggc gcaggggctg ctcctgctga cctgtggcgc
      241 atacggcaac gtgattcgct tcctgtatcc gctgaccatc ccggatgcgc aattcgatgc
      301 ggcaatgaaa attttgcagg atgcgctgag cgattaatag cccaacgcaa taatgtctga
      361 tgcgctgcgc ttatcaggcc tgcaaacgac gtattgatta tgtatgccga ataaagcatt
      421 cacgccgcat ccggcaagtt gtattgctca acttcgctaa atctggtgct ttttcaacaa
      481 cgcgcggaac tgatgataag ttaaccccag taattcagcc gcgcgcttct ggttatattt
      541 cccctgttgc aaactgagtt gcagcaactc tttttcctgc tgcatctgaa actcgcgtaa
      601 atccagcggc agcgtcggaa gcgaggtatt ttctgaaacg gcgatggctt cttcaggtgg
      661 acgccgttta aagggatcaa taatgatgtc atcaagcgga
//
LOCUS       DAZSNM010000510          694 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZSNM010000510 DAZSNM010000000
VERSION     DAZSNM010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.520"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            32..493
                     /locus_tag="ACHW42_004540"
     CDS             32..493
                     /locus_tag="ACHW42_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR8946037.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..>694)
                     /locus_tag="ACHW42_004541"
     CDS             complement(534..>694)
                     /locus_tag="ACHW42_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR8946038.1"
                     /translation="LAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLA
                     ARQHVKKS"
BASE COUNT          204 a          152 c          159 g          179 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaa
//
LOCUS       DAZSNM010000511          693 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZSNM010000511 DAZSNM010000000
VERSION     DAZSNM010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.521"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..525
                     /locus_tag="ACHW42_004542"
                     /pseudo
     CDS             1..525
                     /locus_tag="ACHW42_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            589..>693
                     /locus_tag="ACHW42_004543"
     CDS             589..>693
                     /locus_tag="ACHW42_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR8946039.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDIT"
BASE COUNT          193 a          143 c          166 g          191 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      661 gttgccagcg ttgcaacacg tctcgatatc acc
//
LOCUS       DAZSNM010000512          685 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZSNM010000512 DAZSNM010000000
VERSION     DAZSNM010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.522"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            52..685
                     /locus_tag="ACHW42_004544"
                     /pseudo
     CDS             52..685
                     /locus_tag="ACHW42_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          205 a          172 c          173 g          135 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttga
//
LOCUS       DAZSNM010000513          669 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZSNM010000513 DAZSNM010000000
VERSION     DAZSNM010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.523"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..357
                     /locus_tag="ACHW42_004545"
                     /pseudo
     CDS             1..357
                     /locus_tag="ACHW42_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            361..520
                     /locus_tag="ACHW42_004546"
                     /pseudo
     CDS             361..520
                     /locus_tag="ACHW42_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          177 a          159 c          177 g          156 t
ORIGIN      
        1 cctccggcgg tctggttcgc gtactcaccg gaccggaaag gtatccatcc acagaatcat
       61 ctggccggtt acagcggagt gcttcaggcc gatgcttacg gtggttaccg ggcgttgtac
      121 gaatccggca gaataacgga agccgcatgt atggctcatg tccggagaaa aatccacgat
      181 gtgcatgcaa gagtgccaac cgacatcacc acggaagccc tgcagcgtat cggtgaactg
      241 tatgccatcg aggcagaagt ccggggatgt tcagcagaac agcgtctggc ggcaagaaaa
      301 gccagagccg cgccactgat gcagtcactg tatgactgga tacagcaaca gatgaaaatt
      361 cattcgctga aaatggaatg tctccacgga gaacactatt atccatcggg aaatagtgcg
      421 ggcaacagtg tttagttata tcgagtgcga ttacaatcgc tggcgacggc accgttggtg
      481 tggtggtctc agtccggaac aatttgaaaa ccagagcctc acttacgact gtgtccatat
      541 tatgtgggta ggatcaataa agacgtttta tatgtgtctg cttgcagcca gaaaatttat
      601 accctgcagg tatcataaac atgcttgaca acttaattag ggccccctct tccttttatg
      661 gtgtaattg
//
LOCUS       DAZSNM010000514          656 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZSNM010000514 DAZSNM010000000
VERSION     DAZSNM010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.524"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(151..>656)
                     /locus_tag="ACHW42_004547"
     CDS             complement(151..>656)
                     /locus_tag="ACHW42_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR8946040.1"
                     /translation="PVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDL
                     KELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDP
                     STPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKL
                     VERLMQL"
BASE COUNT          173 a          160 c          144 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DAZSNM010000515          655 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZSNM010000515 DAZSNM010000000
VERSION     DAZSNM010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.525"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            21..566
                     /locus_tag="ACHW42_004548"
     CDS             21..566
                     /locus_tag="ACHW42_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR8946041.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            590..>655
                     /locus_tag="ACHW42_004549"
     CDS             590..>655
                     /locus_tag="ACHW42_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR8946042.1"
                     /translation="MSVVISGALTDGAGIPMSGYHI"
BASE COUNT          193 a          141 c          167 g          154 t
ORIGIN      
        1 aacgtagcga gtatttaaca atgttgcatt taaaaaatat tactgcgggt aatccgaaaa
       61 cagcagaaca atatcagatg acaaaacgat attcggtcac ctggcttttt tcagaagacg
      121 gaaaaaactg gtatgaagag ctgaagaatt tcgccagcga cacaataaaa atagcttaca
      181 ccggagatgg tcgcgtggtg tgggtcggta aggatgtgac aggcatcgag ccacgcaatg
      241 ccagcgttat cgaagttccg gatattaccg ctaaccgacg gattaccgcg ccgggttact
      301 ggttttaccg caatgatgaa tttgtctttg actacagact caaagcggaa gatgagcgtg
      361 atgcccttct ggctcaggtc agtgcccgga caggggaatg ggaagaagac ctgctgctgg
      421 ggttaatcag cgacgaagat aaagaaaagc tgaaagcctg tcgtatttac gcgaaatcgc
      481 tgcaggcgat ggatttcagc accatcactg ataaatcctc atacaacgcc attgaatggc
      541 ccgcctctcc ggaaggttct tcctgattta atttatcgcg agaaaaacaa tgtctgtagt
      601 gatatcaggt gcgctgactg atggcgcagg tatccccatg tccggatacc atatt
//
LOCUS       DAZSNM010000516          651 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZSNM010000516 DAZSNM010000000
VERSION     DAZSNM010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.526"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>651
                     /locus_tag="ACHW42_004550"
     CDS             <1..>651
                     /locus_tag="ACHW42_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HFR8946043.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNGWSLAGEHRH
                     WMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKV"
BASE COUNT          167 a          187 c          185 g          112 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatgagctt gctgagcaac ttatagaaca aaccggcgat aacactctga cgttaatgga
      421 taaaggttat tactcactgg gactgttaaa tggctggagc ctggcgggag aacaccgcca
      481 ctggatgata cctctcagaa agggagcgca atatgaagag ctcagaaaac tgggtaaagg
      541 cgatcatctg gtgaagctga aaaccagccc gcaggcacga aaaaagtggc cgggactggg
      601 aaatgaagtg acagcccgcc tgctgaccgt gacgcgcaaa ggaaaagtct g
//
LOCUS       DAZSNM010000517          650 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZSNM010000517 DAZSNM010000000
VERSION     DAZSNM010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.527"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>650)
                     /gene="wzx"
                     /locus_tag="ACHW42_004551"
     CDS             complement(<1..>650)
                     /gene="wzx"
                     /locus_tag="ACHW42_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HFR8946044.1"
                     /translation="LIFSDGIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAI
                     LEGDEKFGIVNIQKSISSSCIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVR
                     NDIKISGVHFCYKTFKRLFFFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAP
                     AEVILKLGIIPAAIGRAVFPRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLY"
BASE COUNT          245 a          128 c           89 g          188 t
ORIGIN      
        1 atataacaag cctatgatta tcaccggcaa acagattaga aacattaaaa gcaatgattt
       61 atttacatta cgtttaaatt ctttaaagtc tttgatgtta cttaaccttg gaaacactgc
      121 cctcccgatt gctgcaggta ttattcctaa ttttagaata acctccgctg gtgcagaata
      181 aaatgcaact ttatctgccc ccataatatt tgacactata aatcgatcaa aatacaccat
      241 gaccggactt atgatattac ttactgtcat ccaaccgcca aagaaaaaga gtcgtttaaa
      301 ggttttataa caaaaatgaa cccccgaaat tttaatatca ttcctgacat aataggcaga
      361 gactaaaatc gaaataactc ttgcaaatat taaaccagca accgctgccg acaatgtagc
      421 actataaaaa acaaatatgg ccggaattcc cgcgatgcaa gagcttgata tagatttttg
      481 aatatttaca atgccaaatt tttcatcccc ctcaagaatg gctgaccata attgatttag
      541 aataaataat ggaatgcaaa tagccaacag tttaaatgct agttgtatat cactatgttc
      601 aacaccagaa atttttagat aattaacaat tccatcagag aaaatcaata
//
LOCUS       DAZSNM010000518          644 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZSNM010000518 DAZSNM010000000
VERSION     DAZSNM010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.528"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(29..397)
                     /locus_tag="ACHW42_004552"
                     /pseudo
     CDS             complement(29..397)
                     /locus_tag="ACHW42_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          189 a          113 c          144 g          198 t
ORIGIN      
        1 ttgtctggag attcaggggg ccagtctaat cagttgtcat gcactcggaa taaatcatac
       61 cgtgaatagt gctgtttttg attgtcagac tattggcgtt ggtattggta gaaataccgt
      121 cgtccagata gtaggtagaa atcacgccgt taacggtagc gttattaatg accgggtaga
      181 tatcgccgtt gtaatagcta tcagcggcat agttgttcca gcctacataa ccatcatagt
      241 aaacgtcatc agcataggtg gcgtcgttgt agtgatggaa agtattgtaa gtttgaccag
      301 aaatatcgtt ggtcgctgca ttagcctgag aggtgatagc cagagtgcaa gccaatgcta
      361 attgtgatac tacaagtttc tttttccagg agtgcatttg tcatccctcc tcagggacgt
      421 aataagttga tccatcaatt atcaatgcat agaaaacata ttatgtttcg cgctaacgat
      481 tatgctgcgt tacagacgaa tggttcaatt attctttccc ctaagtccga atgtggacat
      541 atataattta tggagaaaaa cacgcatcaa attgataaaa ggacgatatt tcagtatgtt
      601 gcagataatg ttatttttga tgcatacgcg ttataaatat caaa
//
LOCUS       DAZSNM010000519          638 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZSNM010000519 DAZSNM010000000
VERSION     DAZSNM010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.529"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>638
                     /locus_tag="ACHW42_004553"
     CDS             <1..>638
                     /locus_tag="ACHW42_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8946045.1"
                     /translation="MKTITLNDNHIAHLNAKNTTKLEYLNLSNNNLLPTNDIDQLISS
                     KHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTTQPPGLQNFTSIN
                     LDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMNNNALRNIDIDRL
                     SSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELLLLDLSH"
BASE COUNT          240 a          127 c           88 g          183 t
ORIGIN      
        1 atgaaaacaa tcactttaaa cgacaaccat attgcacatt taaacgccaa aaacactaca
       61 aaactggaat atttaaactt aagcaataac aatttactgc caaccaatga cattgatcaa
      121 ctaatatcat caaaacatct ttggcatgta ttagttaacg gcatcaacaa tgatccactt
      181 gcacaaatgc agtactggac tgcagtaaga aatataattg atgacactaa tgaagtgacc
      241 attgatttat caggacttaa tttaaccact caaccaccag ggctgcaaaa cttcacctct
      301 atcaatcttg ataataacca actcacacat tttgatgcaa ccaactacga tagactcgta
      361 aagctaagtc tgaatagtaa tgctcttgag tcaataaatt ttcctcaagg cagaaatgta
      421 agtattacac atatatctat gaataataat gctctcagaa atattgatat agataggctt
      481 tcatcagtta cttattttag tgcggcacat aatcaactag agtttgtgca attagaatct
      541 tgcgaatggc tgcaatacct gaatctcagc cataatcaat taactgatat tgttgcaggt
      601 aataaaaatg aactcttact gctggatcta tcccataa
//
LOCUS       DAZSNM010000520          632 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZSNM010000520 DAZSNM010000000
VERSION     DAZSNM010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.530"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..228
                     /locus_tag="ACHW42_004554"
     CDS             <1..228
                     /locus_tag="ACHW42_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393504.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFR8946046.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
BASE COUNT          226 a          125 c           93 g          188 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaatgac
      601 gccttttatt gataatttac atatgaaaaa at
//
LOCUS       DAZSNM010000521          629 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZSNM010000521 DAZSNM010000000
VERSION     DAZSNM010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.531"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..563)
                     /gene="fimE"
                     /locus_tag="ACHW42_004555"
     CDS             complement(<1..563)
                     /gene="fimE"
                     /locus_tag="ACHW42_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFR8946047.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNL"
BASE COUNT          181 a          163 c          133 g          152 t
ORIGIN      
        1 agattatttc tttcccataa tccggcaaaa cgagcagcat tactggcggt ataacgcaca
       61 gtatggcgaa tatttcgatg cccgagataa tcctgaatta aacgggtatc tgcaccacgc
      121 tccgccagtt cataaccgca agcatgcctt aacatatgag gatgagtctg cgttacggtt
      181 ccagcttcaa taccggcatc gcgaataatg cgataggcct gctggcgaga aagccgactc
      241 ccgcggcgag aaataaatat agcgtcggtc cggtcagcgc ctttccagtt agcacgttcc
      301 tgggtccagc gttccacggc ttcacgctca tcaaaacgta acgggtgaac ggtagaaaat
      361 ccgttcttca gtcggcgaat atttattcga ccttcattaa ggtcaaggtc ctgataatgc
      421 agatcaagca gttcactaat acgcatccca tgccgatatg ccaacagaat aagacaataa
      481 tctctggctc ccgttgcccc gtaacaaacc gcctgcatca tggcctgaac ttctttaccg
      541 gtaagataac gacgtttact cacaatagta gtactcctaa ctgagatata tttgaatgaa
      601 tacctatagg aaacctcaat cggtcaaaa
//
LOCUS       DAZSNM010000522          626 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZSNM010000522 DAZSNM010000000
VERSION     DAZSNM010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.532"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            88..153
                     /locus_tag="ACHW42_004556"
                     /pseudo
     CDS             88..153
                     /locus_tag="ACHW42_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            381..>626
                     /locus_tag="ACHW42_004557"
     CDS             381..>626
                     /locus_tag="ACHW42_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR8946048.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          196 a          129 c          147 g          154 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcac
//
LOCUS       DAZSNM010000523          623 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZSNM010000523 DAZSNM010000000
VERSION     DAZSNM010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.533"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..251)
                     /locus_tag="ACHW42_004558"
     CDS             complement(<1..251)
                     /locus_tag="ACHW42_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFR8946049.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(272..>623)
                     /locus_tag="ACHW42_004559"
     CDS             complement(272..>623)
                     /locus_tag="ACHW42_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8946050.1"
                     /translation="TVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRH
                     RKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRR
                     LSESVPCMPHKKAG"
BASE COUNT          141 a          145 c          141 g          196 t
ORIGIN      
        1 ctttgcaggt aagtcagtaa tgtttcttgc tgatcgcgcc agacgttctg cacatcaaca
       61 ttttcattac tgagcagtat tttaactttg ctgacctgca cgccgttgtc gatccagcgt
      121 ttgatctcgc ggatccggtc aatatcggca tcgttgaaca gccgatgacc gccgtctgtc
      181 cgttgcggtt tcagcaatcc gtaacgcctc tgccacgcgc gtaacgtgac agggattaaa
      241 ggtaaaccaa ttgattttgc aagtttttat tttaccccgc tttcttatgg ggcatgcatg
      301 ggacactttc agatagtctt ctgttaagaa gttctatctg ttcgtgattg ttgtctttca
      361 tccatgctcc gtaaacattg aataccattt gtgcgtttgt gtggcccatc tggcttgcga
      421 taaaactagg attagctcct gcggcaagtg accagcatgc ataggtatgc ctggattgat
      481 acgattttct gtgtctcaga cctgcgcgtt ttaagatact tgtccatgac tccctgatgg
      541 agtcaacctt atagtgcggt ccggaaaact gccgctgttt tattacctga ggactaaaaa
      601 caaaagtgca tttatgcaca gta
//
LOCUS       DAZSNM010000524          619 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZSNM010000524 DAZSNM010000000
VERSION     DAZSNM010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.534"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            51..350
                     /gene="pliG"
                     /locus_tag="ACHW42_004560"
                     /pseudo
     CDS             51..350
                     /gene="pliG"
                     /locus_tag="ACHW42_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
     gene            complement(458..>619)
                     /locus_tag="ACHW42_004561"
     CDS             complement(458..>619)
                     /locus_tag="ACHW42_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR8946051.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          197 a          145 c          117 g          160 t
ORIGIN      
        1 gactgagagt ttactcactt agccgcgtgt ccactattgc tgggtaagat catagcagcg
       61 ctcagtactc cggcgaaata aagggatacg attacgatac atataccttc tatgccaaaa
      121 aaggtcagaa agtgcatgtg agtatctcta atgaaggcgc tgatacttat ctcttcgggc
      181 caggaatcga tgattctgtt gatttatcca gatattcccc agaactcgat agtcacggac
      241 aatactcact ccctgcttct ggcaaatatg agctgcgagt gctgcaaact cgaaacgacg
      301 cccgtaaaaa caaaactaaa aaatataacg tcgatattca aattaagtaa atgtcaaccg
      361 acggggacta tccccgtcat agacgttaac gccccactcc tctctgtcgc tcaagaggtt
      421 aatgacgaat agcagagaac ataatgtaaa caaggcatca ctcacgacac ataaataaca
      481 tctttgtggt ggtttgattg atttttccac tccgcttacc atctacacca aaataacgat
      541 catcacgaca taatttttct ttaacatcgc aaaatacacc gttagcgaat gtaaattcgc
      601 tgggatcgta gccttgtaa
//
LOCUS       DAZSNM010000525          606 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZSNM010000525 DAZSNM010000000
VERSION     DAZSNM010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.535"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..168)
                     /locus_tag="ACHW42_004562"
     CDS             complement(<1..168)
                     /locus_tag="ACHW42_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFR8946052.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAV"
     gene            complement(492..>606)
                     /locus_tag="ACHW42_004563"
     CDS             complement(492..>606)
                     /locus_tag="ACHW42_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8946053.1"
                     /translation="LDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
BASE COUNT          200 a          102 c          125 g          179 t
ORIGIN      
        1 aactgcgcta aatagcatcc agacacagaa ggcaagaagt agacaactga ctgatatcca
       61 gaggtttctt cgagcaatat gttttccttt attttcccag aaggccggat tttctggttt
      121 ccagtcgcgt aaaagataac gactattttt ctcattttgc agtgccatat tgttcctcat
      181 atgcacacat tggtaatgaa aaaagacaaa acaggaggta aggcgcaata gccagttatt
      241 agaattaagg atgaattggg tgaagtgctg attgaaagaa tagataagaa agcgtaaccg
      301 cgggggcaga atggggatta agtagccaga tatgtgttac taaatgtaac taacaagcta
      361 accttcaaac gggggttaat ctttgaacgt atgtcatgtt tcaacccttc agatcgtgaa
      421 tctaaagggt tacatattaa ctatattctc aacaagacca gacacttata gaattagtga
      481 tgatttattc actaaaaagg ccatgaattg gaggaaaacg atctgtccag aaagtctatc
      541 ggttcctctc ttattgcctg attgtaataa tcgttattat agtaagtatt gtttatttca
      601 tctaag
//
LOCUS       DAZSNM010000526          591 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZSNM010000526 DAZSNM010000000
VERSION     DAZSNM010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.536"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            487..>591
                     /locus_tag="ACHW42_004564"
     CDS             487..>591
                     /locus_tag="ACHW42_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8946054.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKN"
BASE COUNT          217 a          105 c          121 g          148 t
ORIGIN      
        1 atcccgtact ctctggccag ttgggccacg caggccccta gctggtaaga tttttcaata
       61 agatcaagct taaactccat agagtcatag agtaagaggc acgaatcctg cgggggcgaa
      121 gagaatctga cattgataga gtccattaac gttataagag gactctatca tcgccaatca
      181 tcagacattg aaatagacga cactgagatg acgcttacaa ttcttttggt ttctgtaata
      241 agtaatctga catcccccgt gcccagaaga ggagagggat atcaagccca agggttaata
      301 attgatacta cggttaaaat tgccttcagt aaaggaaacg tgattaaatt caggggattt
      361 cagagtgaaa accatacaac atattaaaat ctgttcattt agctataata catacaagaa
      421 agtgtccagt tagataatgt tatctaaata accacagata aaaacgcaca taattggggg
      481 ggggggatga aaatacctga agcagtaaat catattaatg ttcagaataa tattgatctt
      541 gttgatggaa aaacgaatcc gaataaagca acaaaagcat tacaaaaaaa t
//
LOCUS       DAZSNM010000527          587 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZSNM010000527 DAZSNM010000000
VERSION     DAZSNM010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.537"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          233 a          137 c           69 g          148 t
ORIGIN      
        1 taaaattcat ataaacgcca ttgctgaata ttatttattt catacagata aacgataaaa
       61 tacaaaaggg tatatttctc aatgtaacag tcaattattc atctccactg gcgagcacgg
      121 tcagcacaca aacaaagtta gtcctcctgc ttaatcattg cgtatcagaa actaaaaaag
      181 aaaaaattct gcagacaact taacaatctt catcacattt cctgaagaca gacatatcca
      241 caaacggtag tcagcgttga attaacaagt cttacagagc tatacgtagc ttataaagcc
      301 actcaatgtt catcatcacc cgactactta tctggaatac caccagtata aactcatcaa
      361 taaaaacacc tgttgttaaa ataagaatac tgtcagcagt aaattattaa aacaacatct
      421 gtccacacta tctgttcttt taacgccaac ggcagcgaaa atagtgcatc acagccttct
      481 tttttctttc gccaccactc agacaaacac aacactcaca cccacaccac aaaacagaat
      541 caaaacataa aattaacaat aacagtcaaa aggaaaacac agaatat
//
LOCUS       DAZSNM010000528          581 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZSNM010000528 DAZSNM010000000
VERSION     DAZSNM010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.538"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(141..>581)
                     /locus_tag="ACHW42_004565"
     CDS             complement(141..>581)
                     /locus_tag="ACHW42_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR8946055.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          147 a          163 c           99 g          172 t
ORIGIN      
        1 caccagcacg tttaacgtcc tgtgccgttt ttcaggtata aaaaaaaccg cataaagcgg
       61 gctctttcaa atgtccatgt ctgctattcg cctcgcggta cagctttgcg aagcgtacaa
      121 gaattgaagc agtttttacg tcaaaaagca ataacttttt tctctatacc aaaagccata
      181 accattggtt tgtacaaaat aaattctgcc acctttagcc aatgctcaat gcgtctttca
      241 caggttctta aactccattt cgggtgtgca tcattcagca gttcagccat tttgcatttg
      301 gtcatccccc gcccctcata ccgttgccga aggacgctaa tcaatcctgg atgctctgcc
      361 agcacctcac ttatgacccg atcaatacat aacgcctctg catcagtaca atgcgccagc
      421 cagctctttt gcttaccgtt aatcatatcc cgcaaaaaag cctcaagttc agacttgttc
      481 agacctgctt ttttcatcct ccggagcgcc tcgttaattg ccgtttttgt cagcttttta
      541 gaggtcaaca tcaggttaaa catattcccc gtcttaccgc c
//
LOCUS       DAZSNM010000529          572 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZSNM010000529 DAZSNM010000000
VERSION     DAZSNM010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.539"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          180 a          123 c           95 g          174 t
ORIGIN      
        1 gtaaaaataa aaaaatacct cattgtgcga ataaagtaac gggggccata gatttctcta
       61 cctacctttt ggtgttcata cctgaggctg atagtttcag gattccgcga caattctact
      121 caccttcttg ttctctcgcc aatatgaaat caatcagaaa agcgttccat aagaacaacg
      181 ataagacaat aaattgtcat cacggctgca atagccaacg tgcctttcag taccaacatg
      241 acaacctcct gtatttgacg taacgtgtgc tgataaatat gtatttgtat ctcaactgac
      301 tcaatacaac acttaagtat agtttttcag attttgtttt tcccgtgaaa tattatggaa
      361 atggcagaag caatacattg aattccgatt cgagtgaata cggctagcgg gcacctcact
      421 ttagcactga agccatcaac ttttatttga cttatttcaa tggaaagctt tctcactatc
      481 agtacaggta attcccatcc ttttaaagtc gagagtcaga ctgcgactaa tacagttaaa
      541 gtgaccaaac tcagttatcc tctacacatt tt
//
LOCUS       DAZSNM010000530          558 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZSNM010000530 DAZSNM010000000
VERSION     DAZSNM010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.540"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..94
                     /locus_tag="ACHW42_004566"
     CDS             <1..94
                     /locus_tag="ACHW42_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFR8946056.1"
                     /translation="IATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            209..>558
                     /locus_tag="ACHW42_004567"
     CDS             209..>558
                     /locus_tag="ACHW42_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8946057.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLA"
BASE COUNT          143 a          153 c          141 g          121 t
ORIGIN      
        1 catcgccacg cgttacgaca aaacggcgag aaattacctg gcgatggtga aactgggctg
       61 catccgactc ttttatcaac gcttacgtaa ttaagggaca cagcctaaat agctgcgcgg
      121 aatagtagat ccctttgagg gaacttagcc cggattgtgc gatctgatca atcgccaaat
      181 caaaacaaat caccaaccgg actgagcaat gccgatcata gcaccaattt cccgtgacga
      241 acgacgcctg atgcagaaag ccatccataa aacacacgat aaaaattatg cccgcagact
      301 gactgccatg ctgatgctgc accggggcga ccgtgtcagc gacgttgcca gaacgctctg
      361 ctgcgcccgt tcctctgttg gacgctggat taactggttc acgcagtcgg gtgttgaggg
      421 actgaaatca ttacctgccg ggcgtgcccg tcgctggccg tttgagcata tctgcacact
      481 gttacgtgag ctggtaaaac attctcccgg cgactttggc taccagcgtt cacgctggag
      541 tacagaactg ctggcaat
//
LOCUS       DAZSNM010000531          556 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZSNM010000531 DAZSNM010000000
VERSION     DAZSNM010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.541"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            183..556
                     /locus_tag="ACHW42_004568"
                     /pseudo
     CDS             183..556
                     /locus_tag="ACHW42_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          132 a          119 c          186 g          119 t
ORIGIN      
        1 gaagggtatc cggaaattgc ccgctgcatg gagcgggaat ttgcgacaat tcctgccggt
       61 atcaacgccg acggcatgta ttgccctgaa tgcggtacgg ttcactggcc ggatggcgtt
      121 attccgcctt tctgatgctt ccccgttttg ccgatatttt tcagcaggga aaccgatggc
      181 ttaactggct ggagaagcag ccggtgcaga tgagccgcct ggaacattat gccggtcagg
      241 atgaaattgg cctgcgttac aacagccacc ggacaaaacg agaagaaaac cttgtgatga
      301 gtggcgacga gtttatggaa aggttctcct ggcatgtggc ggataagggg ttccggatgg
      361 tgatacgggg tcctgagtcc ggtgaagcgg cgattactgg aagatgtggt gtacgccata
      421 acggagacag tgagaaaaac ggcgaggcaa atcacaagga gagggatgta tcagcagtgt
      481 ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt tacggggctg
      541 aagaggggtt accgtc
//
LOCUS       DAZSNM010000532          551 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZSNM010000532 DAZSNM010000000
VERSION     DAZSNM010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.542"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>551)
                     /locus_tag="ACHW42_004569"
     CDS             complement(<1..>551)
                     /locus_tag="ACHW42_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HFR8946058.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIK"
BASE COUNT          134 a          117 c          135 g          165 t
ORIGIN      
        1 atttaatatc aatctgttga aggtgtatta ttacatcagt agcatattcc agactgcctt
       61 cctggacgat ctgatccata acatcctcac gcactttata tcttgtgcat ttcatttcaa
      121 gtgccgtgag tacagccatt actggtgatg gtagctgtgt tgttttcagg ccgtcgatct
      181 gactgatcgc ttgggcgacg gtgttttgcc attctaaaga acaggtgctg aagtcaaatg
      241 tggctgtttt attatcccgt tgcgacacgc tacaggctaa gcgacgtaat tgggtcaatt
      301 tgtctgcttg ctgaacagga actccgggaa agcggcgatg acttaacgtg gtgtgtacaa
      361 cttcgggcgg aaaaacgaca gcaatatctt tcgccagcca aggtaaaaca gcatctgtat
      421 ttttgtcagc cgcacgggtt tctgctacga aagagggttg ttcctgaata atggtggata
      481 agtccgctac cttgagagag tccgttaccg tttgggaagg ttgcaaagaa ttatctttcg
      541 cttgctccac a
//
LOCUS       DAZSNM010000533          551 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZSNM010000533 DAZSNM010000000
VERSION     DAZSNM010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.543"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            144..>551
                     /gene="sxy"
                     /locus_tag="ACHW42_004570"
     CDS             144..>551
                     /gene="sxy"
                     /locus_tag="ACHW42_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR8946059.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT          155 a          105 c          132 g          159 t
ORIGIN      
        1 ggaaagagcc tcgcaaattt tgtcgttggt gacgggaaaa cataaattaa tcttgcccct
       61 taagaataag ttgcctattt tcgtagttaa cggatccgtt aatgtgaatc attcttttat
      121 gttatgattt taaaaggaat tttatgaaaa gcctctccta taagcggatc tataaatcac
      181 aagaatacct ggcaacgttg ggcacaattg aataccgatc attgtttggc agttacagcc
      241 tgaccgttga cgacacggtg tttgcgatgg tttctgatgg tgagttgtat cttcgggctt
      301 gtgagcaaag tgcacagtac tgtgtaaaac atccgcctgt ctggctgaca tataaaaagt
      361 gtggccgatc cgttaccctc aattactatc gggttgatga aagtctatgg cgaaatcaac
      421 tgaagctggt gcgtctgtcg aaatattctc tcgatgcagc gctgaaagag aaaagcacgc
      481 gcaatacccg ggaaagactg aaagatttgc ccaatatgtc ttttcatctg gaagcgattc
      541 tcggggaggt g
//
LOCUS       DAZSNM010000534          548 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZSNM010000534 DAZSNM010000000
VERSION     DAZSNM010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.544"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(282..>548)
                     /locus_tag="ACHW42_004571"
     CDS             complement(282..>548)
                     /locus_tag="ACHW42_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFR8946060.1"
                     /translation="SGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANK
                     EQIEKIYPIVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
BASE COUNT          166 a           95 c           97 g          190 t
ORIGIN      
        1 ccatgcctcc caaaagcaga gtcgctagtg ttatgtaatt aggcggttaa acttcaattc
       61 tcagtgttaa gtttgggtaa acgcttgtaa atggtattgc taatttttta attgactaat
      121 attagaaacg gttagtgtga ttgtatatag atatttgagt gtaaggtatt taaaggggca
      181 ttcagcccct ccgtctattt gtgattgcat ccattgatgt gtgtattatt ttgagcttga
      241 aaaagggagg cgctctttaa taataaataa atctatcaaa gctaaatatt aaatggaagc
      301 cacccttttt tctctgatag ttttattatc accaaaccat ttatttcagc tatcgaatct
      361 ggattcggcc aggaaggtaa actcttcaca attggataaa tcttctctat ttgttcttta
      421 tttgctggtt tgcaattaca gattccatag taattcataa atgcaaccat cctaaaatag
      481 ttgccaccat cccaccaaaa aaaagatttt ccaaaagtgt ctgcgctcat ggagagcata
      541 tttcctga
//
LOCUS       DAZSNM010000535          529 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZSNM010000535 DAZSNM010000000
VERSION     DAZSNM010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.545"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            378..>529
                     /locus_tag="ACHW42_004572"
     CDS             378..>529
                     /locus_tag="ACHW42_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR8946061.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          132 a          140 c          117 g          140 t
ORIGIN      
        1 tagggtctct ctataggtct tctctatggt ctctctaggg tctctctagg gtctcctcta
       61 ggcattaccc ccagaagcta actctatggc cctctatggt cctctatggc cctgtacgtc
      121 attctttagg atgccctgta gtttcgtata gcctgatgcc tagagagacc ctagagagac
      181 cctagagaga ccctagagtg gctcctagag agaccctccc agtagctccg cacagctaca
      241 tcgtcatcat catagtattc aatctcaaag tcattctgtt tgcatcctgt tgtcctgctg
      301 gtctcccttc gcttggggac tttgagtagg tcagtggggg tgcgacttca gggtgcaaac
      361 aaaaaggaaa tcaagcgatg tctcaacaac cacaacaacc acaacaagaa atcagcatca
      421 ttaacctcga ccagcttgtg tccatgacct cagttgaaat tgctgagctg acaggcaagg
      481 agcaccgaaa tgtccttcgt gatattcgta agatgctcga agaacttaa
//
LOCUS       DAZSNM010000536          527 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZSNM010000536 DAZSNM010000000
VERSION     DAZSNM010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.546"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>527)
                     /gene="wzy"
                     /locus_tag="ACHW42_004573"
     CDS             complement(<1..>527)
                     /gene="wzy"
                     /locus_tag="ACHW42_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HFR8946062.1"
                     /translation="ILLNFGLLNFNLIYEHLSLTSEFFFRPDGAFFSKSFYFFGVGAI
                     ISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLLASFKLHKLYLNTIIIILGSVLF
                     IIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLFGRGFGSFILDRLRIEIVPLEIL
                     QKTGVIGVFISLVPM"
BASE COUNT          222 a           89 c           66 g          150 t
ORIGIN      
        1 aacataggaa ctaatgatat aaatacacca ataacgcctg ttttctgaag tatctcaaga
       61 ggtactattt caatccttaa tcgatctaat ataaatgaac caaatcctct tccaaacaag
      121 aacgtcgcta aatcaacatt tttataataa aaatataaat cattaaatct aacagagtca
      181 gaatcttcac tgcgtgatcc gaccatgtaa agcataatta taaatagaac gctgcccaat
      241 attattataa tagtatttaa atatagctta tgtaatttaa aactggctaa taacagtgat
      301 aatgttgtaa aaagtaatac acctcttgat tctgtcaata atatcgcaag cactattatg
      361 agacatttta aatatttttt gtcgacaaaa ctgataatcg caccgacacc aaaaaagtag
      421 aaggatttgg aaaaaaaagc cccatcggga cgaaaaaaga actcgctagt caatgataag
      481 tgttcataaa ttaaattaaa atttaacaaa ccgaaattca acaaaat
//
LOCUS       DAZSNM010000537          493 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZSNM010000537 DAZSNM010000000
VERSION     DAZSNM010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.547"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..443)
                     /locus_tag="ACHW42_004574"
     CDS             complement(<1..443)
                     /locus_tag="ACHW42_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8946063.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQS"
BASE COUNT          148 a           89 c           74 g          182 t
ORIGIN      
        1 gactgttttt tctgcaaaat atcacaggca tacaatcttc tgttttgttc taacggaaac
       61 tcgcggtttt tgaaatccat gaatgagtta tactcattta ttgttatggg aatttcttcg
      121 tattcaaacc cactgcctcg caatgttatt gtatattcag caccgttatc tttaaggctg
      181 aaactgataa gaaaattatc catggcatta acagtttcat tttttgatga aagcacttga
      241 taactatttt tatcaatctg tgttgcgcaa cgaaaggact cccgcaaagt taacagacta
      301 tcataattcg aaacccaatc ttttttctta aatgcatgct gaatattatt aaaaaaatcg
      361 tttgaatgtg tttttgtatg cgatgacttt acaaataact ccaccgcacc aataaaatta
      421 ttgtgtctga tcctgggtaa catatggcta ccttatttta tttggttatt ttatgtcgcc
      481 attaaatatc tca
//
LOCUS       DAZSNM010000538          491 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZSNM010000538 DAZSNM010000000
VERSION     DAZSNM010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.548"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>491
                     /locus_tag="ACHW42_004575"
     CDS             <1..>491
                     /locus_tag="ACHW42_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR8946064.1"
                     /translation="LPPKPIERGYASAGLLARILVSKYMEHIPLYRQSEIYARQGVEL
                     SRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVY
                     VRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKE
                     AGC"
BASE COUNT          124 a          119 c          147 g          101 t
ORIGIN      
        1 acttcctcct aaaccgatcg aacgcggtta tgccagtgca gggttacttg cacggatcct
       61 ggtcagcaaa tatatggaac atatcccttt atatcgccag tcagaaatat acgcgcgaca
      121 gggcgtggag ctgagccgta ataccatggt gcgctgggta tcagaaatgg cagacaaact
      181 ccgtcctctg tatatagcgc tgaatgacta tgttctggag gcaggaaagg tgcacgcaga
      241 tgacactccg gtgaaagtac tggccccggg gaacggaaag acgaaaacgg gtcgtctgtg
      301 ggtatacgtc agggatgatc gtaatgcggg ttcatccctg ccggcagccg tctggttcgc
      361 gtattcggca gatcgcaaag gagaacatcc gcagctccac ctggcaaagt atcagggcgt
      421 actgcaggct gatgcctatg caggttataa cgtactgtac gaaacgggcc gggtgaagga
      481 agccgggtgc c
//
LOCUS       DAZSNM010000539          483 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZSNM010000539 DAZSNM010000000
VERSION     DAZSNM010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.549"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..483
                     /locus_tag="ACHW42_004576"
                     /pseudo
     CDS             1..483
                     /locus_tag="ACHW42_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          177 a           81 c           95 g          130 t
ORIGIN      
        1 tcgagtaata atgaatatga cctgtttaaa aatagagaaa cctcactgtg gaaacgcaaa
       61 gtaccagaaa gcgcagcgct ggcaatgacg ggaacagtat ataaattatt tggtaatcgc
      121 ttactcaacg cccgcaatca gttagataaa aatctcctcg aaatggctat gcaaatggtt
      181 tatggcaaag atatggtgac aaagtccggg gatatattga ttgatataca attaaataag
      241 gatggcagtc tccagagtaa gcactacaca cttgatgtag gtgaagttat aaacagttat
      301 aatattgacc ctctttgtga atgcttcaag tattaatcaa acttacttac atgataaaaa
      361 tcctggtgac gttattagcc tgtatggcac tttagataac attccccttg tagttaagga
      421 atgcacgggt aaaaatcatc aaattaatgt taaagccaaa ttgccacaag aacgaagtga
      481 aaa
//
LOCUS       DAZSNM010000540          479 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZSNM010000540 DAZSNM010000000
VERSION     DAZSNM010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.550"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..211)
                     /locus_tag="ACHW42_004577"
     CDS             complement(<1..211)
                     /locus_tag="ACHW42_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8946065.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETPKSIMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          135 a           98 c           89 g          157 t
ORIGIN      
        1 aacaggatgc tctgactcgc aattcatcca gcagtttatc atttcccata atgtcactta
       61 ccctcatcgg taaaaaaatt gcctttcgac cattacgata cattattgat tttggggttt
      121 cagggaagta atccatttcg actataactg acaggtcatc acgacgtatg actgcgtatt
      181 tgctactaaa tagtttcttt attttttcca cgatgccccc aggtttataa gtacaaacgg
      241 ttatatccac atagagacaa aaatattaat ctgataaata tttatttcac gccgtatatt
      301 tgattgttta atgtgcaagt acaatgactt ttattttttg ttgtgtatat aatcaaatat
      361 atggttattt ttcaccctgc gtattcagca cgcaacaaaa aacccgccga agcgggttta
      421 gtgcgggtgc gttgaggatg cctgacacat gagaggtggc gagggatttc tcccccgcc
//
LOCUS       DAZSNM010000541          473 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZSNM010000541 DAZSNM010000000
VERSION     DAZSNM010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.551"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..473
                     /locus_tag="ACHW42_004578"
                     /pseudo
     CDS             1..473
                     /locus_tag="ACHW42_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          104 a          117 c          147 g          105 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgc
//
LOCUS       DAZSNM010000542          472 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZSNM010000542 DAZSNM010000000
VERSION     DAZSNM010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.552"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            3..>472
                     /locus_tag="ACHW42_004579"
     CDS             3..>472
                     /locus_tag="ACHW42_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFR8946066.1"
                     /translation="MDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAZSNM010000543          465 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZSNM010000543 DAZSNM010000000
VERSION     DAZSNM010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.553"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..465
                     /locus_tag="ACHW42_004580"
                     /pseudo
     CDS             1..465
                     /locus_tag="ACHW42_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          111 a          133 c          136 g           85 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
      421 tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc agaaa
//
LOCUS       DAZSNM010000544          464 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZSNM010000544 DAZSNM010000000
VERSION     DAZSNM010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.554"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(10..291)
                     /locus_tag="ACHW42_004581"
     CDS             complement(10..291)
                     /locus_tag="ACHW42_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFR8946067.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          126 a          115 c          114 g          109 t
ORIGIN      
        1 ttctacgtat caggtcaaaa tcattggcca ttgtggcggt gtctgattca tctcttctat
       61 catcaccgac tcgatgttgt gccagatagc ggtgatgtcc gccattgtga tgccaagcaa
      121 ccccattgcg aaccaacagg cggaaacaac gcccagaccg ctgctgatca ccgcaagccc
      181 gacataatca gaccgacgaa aacggatgtt gagtgtgctg gccagaaaca tcattacggc
      241 actaagaagt tgccagcgaa gaagaaccac gccagtggtg agggtagcca tcaaacaatt
      301 cctctgaaaa gagccgatgc cctggacagc gcggcgttgt tcacgggagg cgtggtacac
      361 tctggctatc gcggggcttg cagaacacaa aaatgaaaca ctctgtttgt ttcattaatt
      421 ttgtgaacta tatcacaatt gattgtttgt tagccatatt aggc
//
LOCUS       DAZSNM010000545          459 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZSNM010000545 DAZSNM010000000
VERSION     DAZSNM010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.555"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..171
                     /locus_tag="ACHW42_004582"
                     /pseudo
     CDS             1..171
                     /locus_tag="ACHW42_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(197..>459)
                     /locus_tag="ACHW42_004583"
     CDS             complement(197..>459)
                     /locus_tag="ACHW42_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HFR8946068.1"
                     /translation="STGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQANSIKSRR
                     VISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT          112 a          109 c          110 g          128 t
ORIGIN      
        1 aatgcgttcc gcttctttgg tggtgtgtcg cgcgaagtgt tgtatgacaa tatgaaaact
       61 gtggttctgc aacgtgacgc atatcagacc ggtcagcacc ggttccatcc ttcgctgtgg
      121 cagttcggca aggagatggg cttctctccc cgactgtgtc gccccttcag gctgagggat
      181 ccccacaaaa ttactgctaa taaaccagca ccatatttcg gtaggttctg gacagatggt
      241 ttagaactgt gctcagtact gttcgtctta aaataagtgg cgagtgtcga agaacattct
      301 tcgctaacgt cagatatgag ataacccgcc gggatttaat gctgttagcc tgatatttca
      361 ggtgtaaccc tttattttca gcgtgataac cgagtaacca cattacgata gtgctcaacg
      421 ttgccagcag actcagaacc agaattcttc ctgttgaac
//
LOCUS       DAZSNM010000546          459 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZSNM010000546 DAZSNM010000000
VERSION     DAZSNM010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.556"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..443)
                     /locus_tag="ACHW42_004584"
     CDS             complement(<1..443)
                     /locus_tag="ACHW42_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8946069.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVV"
BASE COUNT          151 a           98 c           69 g          141 t
ORIGIN      
        1 attactacca caagcgaaat caaaatgcga aaaagaggaa tactcctttt gcgctctact
       61 ttttcaacaa catcttcttg taaatgagaa tcatcataag attgtttact tattgttatg
      121 ttttcactga tgtaaaagcc tactttgggt atcgtaagaa tcacattttc ttcaacacca
      181 atatttttaa aacttttgcg taacagggat atatattggt tcaatgtgtt gtttgacaga
      241 tcaaatgaaa atctctccaa aacgctatca atgattgagt tcctgtcaac cgctccacca
      301 ttactttcca gtaacactgc caaaatacac cccgccgtag ctggaaaatt tatctcatcc
      361 tgggtattta cattcgagag cgagcatttg ttcggatcaa aaataatcgc gtcattgata
      421 atgaaaaaac atctccgctc catgcaggca acaacacaa
//
LOCUS       DAZSNM010000547          459 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZSNM010000547 DAZSNM010000000
VERSION     DAZSNM010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.557"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>459)
                     /locus_tag="ACHW42_004585"
     CDS             complement(<1..>459)
                     /locus_tag="ACHW42_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFR8946070.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          146 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaa
//
LOCUS       DAZSNM010000548          448 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZSNM010000548 DAZSNM010000000
VERSION     DAZSNM010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.558"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..382)
                     /locus_tag="ACHW42_004586"
     CDS             complement(<1..382)
                     /locus_tag="ACHW42_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8946071.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSLL"
BASE COUNT          135 a          106 c           96 g          111 t
ORIGIN      
        1 ttaacagact tgattcatcc tcacaaaata taagtttttc ctggaatgcc catgtctcat
       61 tgaggttcca gatatcaaat tcgaagtcgt gataacgaaa acggatccca ccaaacttat
      121 taaaacgcag ttgtttgacg ggcagttcag ccaatgtctg gaagagttcc tcacgggagc
      181 gaccgatgac gatatcaagg tctgaagaga atccatcgac gccatacaag cctaagtcac
      241 gcaccatccc gccaaaaatc ctggccggac aggaacaggc cagagtatca agtaatactc
      301 tggctttctt atgctcatca ctaagcgtaa aaaaatcggc gatacgctgt tcaagcagtt
      361 gggtgtttga gaccacattc atacagtgat tttagctcat cgtgaaggcg cattaaataa
      421 ctatccgtca tgcctgagat tgcgtcgg
//
LOCUS       DAZSNM010000549          434 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZSNM010000549 DAZSNM010000000
VERSION     DAZSNM010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.559"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            35..434
                     /locus_tag="ACHW42_004587"
                     /pseudo
     CDS             35..434
                     /locus_tag="ACHW42_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAZSNM010000550          431 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZSNM010000550 DAZSNM010000000
VERSION     DAZSNM010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.560"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            201..>431
                     /locus_tag="ACHW42_004588"
     CDS             201..>431
                     /locus_tag="ACHW42_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR8946072.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          138 a          101 c           59 g          133 t
ORIGIN      
        1 cttaccaacg cgttgtcagt catctccccc ctctctggtg catcgttata tccgtgatga
       61 gcgatttttg atattaatat ctttcaccat ccgctatttt caccacttca gaaaacaaac
      121 ttatttttat taaaatcaat tctttttctt tgaacattca actgcgatat ttgtaaaaac
      181 attaaacagg caacaataaa atgacattac caacaactat ttattcattc cccgcatatt
      241 taccccgttt ttcgtccacc gacaaaccag caaaattaaa atttcatcaa tacgcacgcg
      301 ctacattact gtcaaatcgt ggaagagatc ataattgcga tagcagacgt actgttgaga
      361 tccacaaact agatctctct gactggcaag cattcaataa gttagcaacg aagtgtaatg
      421 cttacgatgg a
//
LOCUS       DAZSNM010000551          424 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZSNM010000551 DAZSNM010000000
VERSION     DAZSNM010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.561"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..78
                     /locus_tag="ACHW42_004589"
     CDS             <1..78
                     /locus_tag="ACHW42_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8946073.1"
                     /translation="YISYNKSSLNNLVFSRFIDRLNESF"
     gene            225..424
                     /locus_tag="ACHW42_004590"
                     /pseudo
     CDS             225..424
                     /locus_tag="ACHW42_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT          120 a           94 c           68 g          142 t
ORIGIN      
        1 tacatttcct ataataaatc ttctttaaat aatttggtgt tctcccgatt tattgaccgt
       61 ctaaatgaga gtttttgata tataactata gacagctact ataactttca tctatttatt
      121 cacagcgcga atgaaaaata aaacactctg ctttacagtg tttactttat aattaacagt
      181 cttatctcac tgttcaccct gtaacgttca gagagcgtct tcagatgtct atttataaaa
      241 ttcctcttcc gctcaatatt ctggaagctg cacgagagcg tattacctag acactcaaca
      301 ccctaccccg cgtttgtgtc tcattttctg gcggcaaaga ctctggttta atgctgcatc
      361 tgacagccga acttgcccga caaatgggca aaaaaatctg cgttttgttt atcaactggg
      421 aggc
//
LOCUS       DAZSNM010000552          416 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZSNM010000552 DAZSNM010000000
VERSION     DAZSNM010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.562"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..373
                     /gene="istB"
                     /locus_tag="ACHW42_004591"
     CDS             <1..373
                     /gene="istB"
                     /locus_tag="ACHW42_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFR8946074.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT          121 a           99 c          105 g           91 t
ORIGIN      
        1 aacagcagca gatctgttac ttcagttatc tacggcacaa cgtcagggcc gttataaaac
       61 gacgcttcag cgtggagtaa tggccccccg cctgctcatc attgatgaaa taggctatct
      121 gccgttcagt caggaagaag caaaactgtt cttccaggtc atcgctaaac gttacgaaaa
      181 gagcgcaatg atcctgacat ccaatctacc gttcgggcag tgggatcaaa cgttcgccgg
      241 tgatgcagca ctgacctcag cgatgctggg ccgtatctta caccactcac atgtcgttca
      301 aatcaaagga gaaagctatc gactcagaca gaaacgaaag gccggggtta tagcagaagc
      361 taatcctgag taaaacggtg gatcaatatt gggccgttgg tggagatata agtgga
//
LOCUS       DAZSNM010000553          411 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZSNM010000553 DAZSNM010000000
VERSION     DAZSNM010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.563"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..269
                     /locus_tag="ACHW42_004592"
     CDS             <1..269
                     /locus_tag="ACHW42_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR8946075.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>411
                     /gene="tnpB"
                     /locus_tag="ACHW42_004593"
     CDS             266..>411
                     /gene="tnpB"
                     /locus_tag="ACHW42_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR8946076.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGR"
BASE COUNT           99 a          111 c          116 g           85 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc g
//
LOCUS       DAZSNM010000554          409 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZSNM010000554 DAZSNM010000000
VERSION     DAZSNM010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.564"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            155..340
                     /locus_tag="ACHW42_004594"
                     /pseudo
     CDS             155..340
                     /locus_tag="ACHW42_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          118 a           97 c          108 g           86 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaata gtttgctgag
       61 gcgactggaa acagaagggc aagatcgatt ccgacagcag tgatggtcag ctacctcatc
      121 gataactttg caacagtgcc cttcttgaag catcatgctt catgaagaaa aactggcacg
      181 tcatcaacgt aaacaggcga tgtatacccg aatggcagcc ttcccggcgg tgaaaacgtt
      241 cgaagagtat gacttcacgt tcgccaccgg agcaccgcag aagcagctcc agtcgttacg
      301 ctcactcagc ttcatagagc gcaatgaaaa catagtttta caggggacat ctgacatcac
      361 aaatccccgg gtggggattt gtgtataaga gacagcccat ggaggaagg
//
LOCUS       DAZSNM010000555          404 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZSNM010000555 DAZSNM010000000
VERSION     DAZSNM010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.565"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..395)
                     /gene="ospB"
                     /locus_tag="ACHW42_004595"
     CDS             complement(<1..395)
                     /gene="ospB"
                     /locus_tag="ACHW42_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFR8946077.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDT"
BASE COUNT          119 a           73 c           46 g          166 t
ORIGIN      
        1 tcagtatcgt ttactgtttt tattttcgct ttttttacag aaaaataagc agcagcattc
       61 aatgcgtcat catcattatt tattactatt ggttcactct gtggtagcca tgattcagcc
      121 atttttagaa aatcattttt acttttttca tttttaaaaa cataatcata taacatcttg
      181 ttcttaaata ctcttggtat ttgttgtccc ataatagata gaacatcatt gctatcacaa
      241 aaacctttct ctcctaaaaa aaccaatgct gcttttgggt gagtacatga tgaatttttt
      301 acccttttta ttatatgtgc aaatgcaata tctgatatgc tttcattctt tttattgact
      361 attctacagt atggtctaac accatctaaa ttcataccta catg
//
LOCUS       DAZSNM010000556          400 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZSNM010000556 DAZSNM010000000
VERSION     DAZSNM010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.566"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..400)
                     /locus_tag="ACHW42_004596"
                     /pseudo
     CDS             complement(1..400)
                     /locus_tag="ACHW42_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT          123 a           55 c           75 g          147 t
ORIGIN      
        1 aggtcatgtt tttaactttt atattcctct ggtttacaac ttcctctgtc ttgtaagtgt
       61 gtatattata tggggtatat gcttttttaa tagtatagtg aaacaattcg ttatccgcat
      121 ctattccgtc agagtgaact tcaaatgcac caggcgaaag aattgccagg ccagtattaa
      181 gcttaagtgc tggggaaaac aaattaaata acatgagctg catagtaggt caattgttct
      241 tcccagaaac atttaaaaaa tttaagtttt tgctctgttt tattatgggg aataaagttc
      301 caatatcgga tgtattatta gtatcaatgt gggttatttt attattgtcc agtcttaaac
      361 ttttgataga tggaagataa gtcaggaaat caagatttat
//
LOCUS       DAZSNM010000557          388 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZSNM010000557 DAZSNM010000000
VERSION     DAZSNM010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.567"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(40..>388)
                     /locus_tag="ACHW42_004597"
     CDS             complement(40..>388)
                     /locus_tag="ACHW42_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8946078.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           89 c           87 g          120 t
ORIGIN      
        1 ttcgtgaagc tgttgaaggg tggattctta acggtggcgt taatcagaaa tgctcctgat
       61 gtgccagaac aatttatcag cgtgaagctc atacgctttg cgctgagcct ctatccagtc
      121 gtgtttattg tatacggaca ttatcccgcc aagttcatga ccgagcattc gctcagtaac
      181 atgaggctcg acattcatct cggacagccg tgtaacaagc gttcttctga aatcatgtgt
      241 tcgccagtaa cctagatcca tccctcccct tattctattt acgaatcggt tggctgcagc
      301 gatgctgatc ggtttgttta tgtcttctcc agggaaaaga acatcattgt acattgtcat
      361 ggccttttcg agaaaaggct taatttgt
//
LOCUS       DAZSNM010000558          383 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZSNM010000558 DAZSNM010000000
VERSION     DAZSNM010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.568"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..87
                     /locus_tag="ACHW42_004598"
     CDS             <1..87
                     /locus_tag="ACHW42_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR8946079.1"
                     /translation="RMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            102..>383
                     /locus_tag="ACHW42_004599"
     CDS             102..>383
                     /locus_tag="ACHW42_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR8946080.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           93 a           79 c          118 g           93 t
ORIGIN      
        1 cgcatggtca tgaacggcgg gcgctgggtt aattttaact gggagagctg gcgtccgaat
       61 gttagccatt cgagggttgt taagtagttt caggaggatt tatggcgaaa ccttttactc
      121 ccgaacagcg ggaagaactg aagacgcgaa ttgtggaact cgtgcatcag gacggtcggg
      181 tcacgattcg gcagttgtcc gatgaaacag gtatcagtcg tgcgtctgtc ggtcgcttat
      241 gcatagaact ggtcgcaagt ggtgatgtat ataattctgg ctacggctta ttcccgtctg
      301 aacaggctcg caaggactgg caaagcgccc gcaaaaaact ctcgagagta aaggtgagga
      361 aaccggttgt tgttgatccg gac
//
LOCUS       DAZSNM010000559          383 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZSNM010000559 DAZSNM010000000
VERSION     DAZSNM010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.569"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(273..>383)
                     /locus_tag="ACHW42_004600"
     CDS             complement(273..>383)
                     /locus_tag="ACHW42_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HFR8946081.1"
                     /translation="VNSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          107 a           88 c           77 g          111 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactgtggg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactatt tac
//
LOCUS       DAZSNM010000560          372 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZSNM010000560 DAZSNM010000000
VERSION     DAZSNM010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.570"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..311)
                     /locus_tag="ACHW42_004601"
     CDS             complement(<1..311)
                     /locus_tag="ACHW42_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8946082.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAZSNM010000561          371 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZSNM010000561 DAZSNM010000000
VERSION     DAZSNM010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.571"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>371
                     /locus_tag="ACHW42_004602"
     CDS             <1..>371
                     /locus_tag="ACHW42_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR8946083.1"
                     /translation="ITESKIYSAMQKMTNRRHEENWKLRAESCRKKGKPVPEYTPKPA
                     SVATKATHLSFIKALLRAAEREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECP
                     EPLKSVVEFALATGLRRSNII"
BASE COUNT          131 a           82 c           84 g           74 t
ORIGIN      
        1 acattactga atcaaaaatt tattcagcaa tgcagaaaat gacgaaccgg cgtcatgagg
       61 aaaactggaa actcagggca gaatcatgca gaaaaaaagg gaaacctgtt ccagaataca
      121 cgccaaaacc agcgtccgtt gcaacgaagg ctacgcatct ttcatttata aaggccctac
      181 taagagccgc agagcgtgaa tggaaaatgc tggataaggc accaattatt aaagtgcctc
      241 aaccaaagaa taaacggatc cgctggctgg agccccatga agcacaaagg ctgattgatg
      301 aatgtccgga gccattaaag tctgttgttg aatttgcact ggcaacaggc ttaagacgct
      361 cgaacatcat c
//
LOCUS       DAZSNM010000562          359 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZSNM010000562 DAZSNM010000000
VERSION     DAZSNM010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.572"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..226
                     /locus_tag="ACHW42_004603"
     CDS             <1..226
                     /locus_tag="ACHW42_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR8946084.1"
                     /translation="SPYLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYG
                     TPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            148..>359
                     /locus_tag="ACHW42_004604"
     CDS             148..>359
                     /locus_tag="ACHW42_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR8946085.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGF"
BASE COUNT          115 a           79 c           93 g           72 t
ORIGIN      
        1 atctccttac ctcacaacac gacaagcctg cattgcggct cttcagtctc cgctgcatac
       61 tgtccaggtg agcgcgggtg atggcataac agaggaaaga aaatgtcact cttccgcaga
      121 ggtgaaatat ggtacgccag tttcacattg ccgaacggta aaagatttaa acagtctctt
      181 ggaacaaagg acaaaaggca ggcgacagaa ctccatgaca agctaaaggc tgaagcatgg
      241 cgggtcagca aacttggtga aatacctgat ataacgttcg aggaagcgtg tgtcaggtgg
      301 cttgaagaga aagcacataa aaaatcactg gacgatgaca aaagccggat cggattctg
//
LOCUS       DAZSNM010000563          345 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZSNM010000563 DAZSNM010000000
VERSION     DAZSNM010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.573"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          121 a           62 c           59 g          103 t
ORIGIN      
        1 aaatatatca gggtttgtta ctaataactt ctgaaaacag aaaaaaatag agcagtaatt
       61 ctatttcaga gaggagtaac tccatctatc ctcgatacgt actgaacgtt atcgtgatat
      121 attcatcatg ctttcttcac cattattcga gtatagggag agacgcaatg accttaaata
      181 cctttttatg gctgaattac cattctcagg aataacagca gactccttcc catagacggc
      241 ttagggggaa agcaataccc ggagaaaggg tacaaagcaa actgttttaa aattaataag
      301 ccatattcta tttaactaga attcttatcg ttgataagaa ttcta
//
LOCUS       DAZSNM010000564          344 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZSNM010000564 DAZSNM010000000
VERSION     DAZSNM010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.574"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>344
                     /locus_tag="ACHW42_004605"
     CDS             <1..>344
                     /locus_tag="ACHW42_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR8946086.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAZSNM010000565          343 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZSNM010000565 DAZSNM010000000
VERSION     DAZSNM010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.denovo.575"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            99..>343
                     /locus_tag="ACHW42_004606"
     CDS             99..>343
                     /locus_tag="ACHW42_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR8946087.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIP"
BASE COUNT          108 a           52 c           54 g          129 t
ORIGIN      
        1 ttaatattga actcgttgat taataatcat aaactttcag gcattcatat ttgcaatgag
       61 tgtttaaaaa tcacacaaaa ctggagagcc tactttaaat gtataataac aaaatcgaca
      121 gcaacagaaa aataagacat gattgggttg atgctttaaa atttctcggt atttttgcta
      181 tatatttagg tcaccttggg ttgggggctg gcaaattgta tccttttgtt tttagttatc
      241 acgtcccact attctttttc gctgctggtt tttttactat caaaaaaaat gacttatctg
      301 ttttcgatta tatcaagtca aagttttaca gattaatgat tcc
//
LOCUS       DAZSNM010000566         3290 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.guided.297, whole genome shotgun sequence.
ACCESSION   DAZSNM010000566 DAZSNM010000000
VERSION     DAZSNM010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.guided.297"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..155
                     /gene="frdD"
                     /locus_tag="ACHW42_004607"
     CDS             <1..155
                     /gene="frdD"
                     /locus_tag="ACHW42_004607"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR8946088.1"
                     /translation="LMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFYGLAAILTVVTL
                     IGVVTI"
     gene            218..1351
                     /locus_tag="ACHW42_004608"
     CDS             218..1351
                     /locus_tag="ACHW42_004608"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR8946089.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1440..1973
                     /gene="blc"
                     /locus_tag="ACHW42_004609"
     CDS             1440..1973
                     /gene="blc"
                     /locus_tag="ACHW42_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR8946090.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1970..2287)
                     /gene="sugE"
                     /locus_tag="ACHW42_004610"
     CDS             complement(1970..2287)
                     /gene="sugE"
                     /locus_tag="ACHW42_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR8946091.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2463..2609)
                     /gene="ecnB"
                     /locus_tag="ACHW42_004611"
     CDS             complement(2463..2609)
                     /gene="ecnB"
                     /locus_tag="ACHW42_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR8946092.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2720..2845)
                     /gene="ecnA"
                     /locus_tag="ACHW42_004612"
     CDS             complement(2720..2845)
                     /gene="ecnA"
                     /locus_tag="ACHW42_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR8946093.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(2897..>3290)
                     /locus_tag="ACHW42_004613"
     CDS             complement(2897..>3290)
                     /locus_tag="ACHW42_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR8946094.1"
                     /translation="TDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
BASE COUNT          841 a          850 c          817 g          782 t
ORIGIN      
        1 tcctgatgat cgttctgccg ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg
       61 atctgaaaat ccacgtgcct gcgggcaaat gggttttcta cggtctggct gctatcctga
      121 cagttgtcac gctgattggt gtcgttacaa tctaacgcat cgccaatgta aatccggccc
      181 gcctatggcg ggccgttttg tatggaaacc agaccttatg ttcaaaacga cgctctgcgc
      241 cttattaatt accgcctctt actccacatt tgctgcccct caacaaatca acgatattgt
      301 gcatcgcaca attaccccgc ttatagagca acaaaagatc cccggtatgg cggtggcggt
      361 aatttatcag ggtaaacctt attactttac ctggggctat gcggacatcg ccaaaaagca
      421 gcccgtcaca cagcaaacgt tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt
      481 gcttggtggc gacgctattg ctcgagggga aatcaagtta agcgatcccg caacaaaata
      541 ctggcctgaa cttaccgcta aacagtggaa tgggatcaca ctattacatc ttgcgaccta
      601 caccgctggc ggcctgccat tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct
      661 gcgcttctat caaaactggc agcctgcatg ggctccagga acacaacgtc tgtatgccaa
      721 ctccagtatc ggtttgttcg gcgcactggc tgtgaagccg tctggtttga gttttgagca
      781 ggcgatgcaa actcgtgtct tccagccact caaactcaac catacgtgga ttaatgtacc
      841 tcccccagaa gaaaagaatt acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc
      901 gccaggggcg ttagatgctg aagcttatgg tgtgaagtcg accattgaag atatggcccg
      961 ctgggtgcaa agcaatttac ccccccttga tatcaatgag aaaacgcttc aacaagggat
     1021 acaactggca caatctcgct actggcaaac cggcgatatg tatcagggcc tgggctggga
     1081 aatgctggac tggccggtca atcctgacag catcattaac ggcagtgaca ataaaattgc
     1141 actggcagca cgcccggtaa aagcgattaa gcccccaact cctgcagtac gcgcatcatg
     1201 ggtacataaa acgggggcga ccggcggatt tggtagctat gtcgcgttta ttccagaaaa
     1261 agagctgggt atcgtgatgc tggctaacaa aaactatccc aatccagcga gagtcgccgc
     1321 cgcctgtcag attctcaacg ctctacagta aaatttcatc gggtccgaat tttcggacct
     1381 tttctgcgct tttccttgct gtcatctaca cttagaaaaa aaccagtaag gaaacattta
     1441 tgcgcctgct ccctctcgtt gccgcagcga cagctgcatt tctggtcgtt gcctgtagtt
     1501 ttcctacgcc gccgcgtggc gtgaccgtag tgaataattt cgacgccaaa cgttatctcg
     1561 gtacctggta tgagattgcc cgttttgatc accgttttga acgtggactg gaaaaagtca
     1621 ccgcaacata cagcctgcgt gatgacggcg gcctgaatgt cattaataaa ggctataacc
     1681 ctgacagagg aatgtggcag cagagtgaag ggaaagcgta ctttaccggc gcaccaactc
     1741 gcgctgcgct gaaagtgtca ttctttggtc ctttctatgg cggttataac gttattgcac
     1801 tcgatcggga ataccgccat gcgctggttt gcggcccgga ccgcgactac ctgtggatac
     1861 tctcccgcac gccaaccatt tctgacgaag tgaaacagga gatgctggca gtcgcgaccc
     1921 gggaagggtt tgatgtcagt aagtttattt gggtacagca gcccggtagt tagtgagtgc
     1981 tgagtttcag accaataatc cccaatacga ttaacgccag actcgccaga cgcatcgggt
     2041 tagcggactc accgagcagc acaatgccgg tgatggccgc gccgactgcg ccaatacccg
     2101 tccacacggc ataagccgtc cctaccggta acgatttcat cgcccaggca agtagcgcca
     2161 tactgactat catcgccgtc acggtaataa cactcggcgt caaacggcta aagccgtggg
     2221 tatatttcag gccaacggcc cataccactt ccagcagacc agcaataact aagataatcc
     2281 aggacatatc aggctccgga aatttggggc cgtccccggt gaaaagaggc gtttgcaggt
     2341 cgtcctgcaa agctgatggt tgaacggata ttttgccagg tggcagggga atttcaacta
     2401 ttacctttat gacgatcgca gaaaaaacaa acggcacgac actgctgtcg taccgttatt
     2461 gcttattgct gcgctttcgt tgctgcgcca gaaatcgcgt taccgccatc agaaatgtct
     2521 tcaccaacgc cacgcgtggt gttgcaggca gttaatactg ttgaaagcac cagaacagaa
     2581 aagatcgctg caattgtctt cttcaccata acgtcttcct tttaggtaat gttatttatg
     2641 tttgcctata gcaaattaat aatagacaac atcgccaaaa atgatggaaa tctgatgatt
     2701 tttcggaaga gaagaaaaat tagctggcag cgcgagagat ggagttaccg agatgtttga
     2761 tgtcttcgcc gaaaccgcaa gcggtgttac agcccgtgag cagcgtgctg gcaagcaaaa
     2821 caagaacgat aaggcgtttc atcatctctg acctgaataa gtgatggtgc agcctgcagg
     2881 ccgcaccaca accgcattac ttcacgcgag agacgtattc accagagcgg gtatccactt
     2941 tgatgacttc gccgatttgt acgaacagcg gaactttaac cacagcgcca gtagacaggg
     3001 tagccggttt gccgccagta cctgcggtat cacctttcag gcccggatcg gtatcaacga
     3061 tttccagttc aacgaagttc ggcggagtaa cggagatcgg ctgaccattc cacagagtta
     3121 cgatacactc tgcctgatcc agcagccatt tagcattgtc gccaattgct tttgcatcag
     3181 cagacagctg ctcgaaagtt tcgttgttca tgaagtgcca gaactcaccg tcgttgtaca
     3241 ggtaagtcag gttcatatcg acaacatcag cgccttcagc ggaatcagta
//
LOCUS       DAZSNM010000567         3221 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.guided.303, whole genome shotgun sequence.
ACCESSION   DAZSNM010000567 DAZSNM010000000
VERSION     DAZSNM010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.guided.303"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..524)
                     /gene="intI2"
                     /locus_tag="ACHW42_004614"
     CDS             complement(<1..524)
                     /gene="intI2"
                     /locus_tag="ACHW42_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HFR8946095.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIP"
     gene            856..1329
                     /gene="dfrA1"
                     /locus_tag="ACHW42_004615"
     CDS             856..1329
                     /gene="dfrA1"
                     /locus_tag="ACHW42_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR8946096.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1424..1948
                     /gene="sat2"
                     /locus_tag="ACHW42_004616"
     CDS             1424..1948
                     /gene="sat2"
                     /locus_tag="ACHW42_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR8946097.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            2006..2841
                     /gene="aadA1"
                     /locus_tag="ACHW42_004617"
                     /pseudo
     CDS             2006..2841
                     /gene="aadA1"
                     /locus_tag="ACHW42_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            2870..>3221
                     /locus_tag="ACHW42_004618"
     CDS             2870..>3221
                     /locus_tag="ACHW42_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8946098.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSG"
BASE COUNT          900 a          732 c          759 g          830 t
ORIGIN      
        1 gggattaggc gcgtgggcag taggctgttt ctgcttttcc cacccttacc gtcatgcaca
       61 gtgatgcagc cattatcaaa atcaaaatct ttaacccgca aacgcaagca ttcattaatg
      121 cgcaaacctg caccatacag cagcgtaaaa ataacttggt tgcgagtatc cataacctgc
      181 aaaatgcgtt gcacttcatt tgcagagata acagagggta gccgtctagg cttgcttgca
      241 gggatataat caatatcgcc caacggctgt tgtaaaaacc tgttgtacaa aaaagctagg
      301 gcatttaaag cgattttctg cgtgtttatg gctacatgtc tgctgtttgc taagctggat
      361 aaaaacagcc tgacctcttc actgcccatg gtctgaggat gacgtttttt gtgaaacaga
      421 ataaaacgct taatccagtg caggtaagtt ttttcagttt tcagcgcata acctttttgt
      481 cgcatatccg tgcgtataga atttaaaaat ggactgttag acataaaacg ctccttgtct
      541 tgcaactgtc tgcctataca gcctattcta gctgggattt aaaaaagtgc ctgtttttta
      601 cgcctagaga tgcttgttta ccggtagagt tttaatttaa tgctaaataa attaaaatgt
      661 tatgagttct ttgggtgaga taatgtgcat cgtgcaagca ggatagacgg catgcacgat
      721 ttgtaataac agagtgtctt gtatttttaa agaaagtcta tttaatacaa gtgattatat
      781 taattaacgg taagcatcag cgggtgacaa aacgagcatg cttactaata aaatgttaac
      841 ctctgaggaa gaattgtgaa actatcacta atggtagcta tatcgaagaa tggagttatc
      901 gggaatggcc ctgatattcc atggagtgcc aaaggtgaac agctcctgtt taaagctatt
      961 acctataacc aatggctgtt ggttggacgc aagacttttg aatcaatggg agcattaccc
     1021 aaccgaaagt atgcggtcgt aacacgttca agttttacat ctgacaatga gaacgtattg
     1081 atctttccat caattaaaga tgctttaacc aacctaaaga aaataacgga tcatgtcatt
     1141 gtttcaggtg gtggggagat atacaaaagc ctgatcgatc aagtagatac actacatata
     1201 tctacaatag acatcgagcc ggaaggtgat gtttactttc ctgaaatccc cagcaatttt
     1261 aggccagttt ttacccaaga cttcgcctct aacataaatt atagttacca aatctggcaa
     1321 aagggttaac aagtggcagc aacggattcg caaacctgtc acgccttttg taccaaaagc
     1381 cgcgccaggt ttgcgatccg ctgtgccagg cgttaggcgt catatgaaga tttcggtgat
     1441 ccctgagcag gtggcggaaa cattggatgc tgagaaccat ttcattgttc gtgaagtgtt
     1501 cgatgtgcac ctatccgacc aaggctttga actatctacc agaagtgtga gcccctaccg
     1561 gaaggattac atctcggatg atgactctga tgaagactct gcttgctatg gcgcattcat
     1621 cgaccaagag cttgtcggga agattgaact caactcaaca tggaacgatc tagcctctat
     1681 cgaacacatt gttgtgtcgc acacgcaccg aggcaaagga gtcgcgcaca gtctcatcga
     1741 atttgcgaaa aagtgggcac taagcagaca gctccttggc atacgattag agacacaaac
     1801 gaacaatgta cctgcctgca atttgtacgc aaaatgtggc tttactctcg gcggcattga
     1861 cctgttcacg tataaaacta gacctcaagt ctcgaacgaa acagcgatgt actggtactg
     1921 gttctcggga gcacaggatg acgcctaaca attcattcaa gccgacaccg cttcgcggcg
     1981 cggcttaatt caggagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca
     2041 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     2101 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     2161 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     2221 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     2281 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     2341 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     2401 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     2461 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     2521 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     2581 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     2641 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctaggc aggcttatct
     2701 tggacaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg ttcactacgt
     2761 gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta acaattcgtt caagccgacg
     2821 ccgcttcgcg gcgcggctta actcaagcgt tagagagctg gggaagacta tgcgcgatct
     2881 gttgaaggtg gttctaagcc tcgtacttgc gatggcatcg gggcaggcac ttgctgacct
     2941 gccaattgtt ttagtggatg aagctcgtct tccctatgac tactccccat ccaactacga
     3001 catttctcca agcaactacg acaactccat aagcaattac gacaatagtc catcaaatta
     3061 cgacaactct gagagcaact acgataatag ttcatccaat tacgacaata gtcgcaacgg
     3121 aaatcgtagg cttatatata gcgcaaatgg gtctcgcact ttcgccggct actacgtcat
     3181 tgccaacaat gggacaacga acttcttttc cacatctggc a
//
LOCUS       DAZSNM010000568         3180 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.guided.305, whole genome shotgun sequence.
ACCESSION   DAZSNM010000568 DAZSNM010000000
VERSION     DAZSNM010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.guided.305"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..342
                     /locus_tag="ACHW42_004619"
     CDS             <1..342
                     /locus_tag="ACHW42_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HFR8946099.1"
                     /translation="AATFFEPVPKEHFSKALFDTIAQWNAESDWKGDERNVVLALARI
                     WYSASTGLIAPKDVAAAWVSERLPAEHRPLICKARAAYLGSEDDDLAMRVEETAAFVR
                     YAKATIERILR"
     gene            548..895
                     /locus_tag="ACHW42_004620"
     CDS             548..895
                     /locus_tag="ACHW42_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR8946100.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            889..1728
                     /gene="sul1"
                     /locus_tag="ACHW42_004621"
     CDS             889..1728
                     /gene="sul1"
                     /locus_tag="ACHW42_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR8946101.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            1856..2059
                     /locus_tag="ACHW42_004622"
     CDS             1856..2059
                     /locus_tag="ACHW42_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8946102.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            2215..>3180
                     /gene="chrA"
                     /locus_tag="ACHW42_004623"
     CDS             2215..>3180
                     /gene="chrA"
                     /locus_tag="ACHW42_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFR8946103.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPF"
BASE COUNT          589 a          887 c          938 g          766 t
ORIGIN      
        1 gcagccacgt ttttcgagcc ggtgccgaag gagcatttct ccaaggcgct tttcgacact
       61 attgcccagt ggaatgcaga gtcggattgg aagggtgacg agcggaacgt cgttcttgct
      121 cttgctcgca tttggtacag cgcttcaact ggtctcattg ctcctaagga cgttgctgcc
      181 gcatgggtat cggagcgttt gcctgccgag catcggcccc tcatctgcaa ggcacgcgcg
      241 gcgtacctgg gtagcgagga cgacgaccta gcaatgcgcg tcgaagagac ggccgcgttc
      301 gttcgatatg ccaaagcaac gattgagaga atcttgcgtt gagcggcatg tgcgaaaagt
      361 gcatcgaccc gcgccgaggg catctgatgc ctaactcggc gttcaagcgg acgggctgcg
      421 cccgccgctc aactatgcgt tagatgcact aagcacataa ttgctcacag ccaaactatc
      481 aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag
      541 atatatcatg aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac
      601 atccgcatta aaatctagcg agggctttac taagcttgcc ccttccgccg ttgtcataat
      661 cggttatggc atcgcatttt attttctttc tctggttctg aaatccatcc ctgtcggtgt
      721 tgcttatgca gtctggtcgg gactcggcgt cgtcataatt acagccattg cctggttgct
      781 tcatgggcaa aagcttgatg cgtggggctt tgtaggtatg gggctcataa ttgctgcctt
      841 tttgctcgcc cgatccccat cgtggaagtc gctgcggagg ccgacgccat ggtgacggtg
      901 ttcggcattc tgaatctcac cgaggactcc ttcttcgatg agagccggcg gctagacccc
      961 gccggcgctg tcaccgcggc gatcgaaatg ctgcgagtcg gatcagacgt cgtggatgtc
     1021 ggaccggccg ccagccatcc ggacgcgagg cctgtatcgc cggccgatga gatcagacgt
     1081 attgcgccgc tcttagacgc cctgtccgat cagatgcacc gtgtttcaat cgacagcttc
     1141 caaccggaaa cccagcgcta tgcgctcaag cgcggcgtgg gctacctgaa cgatatccaa
     1201 ggatttcctg accctgcgct ctatcccgat attgctgagg cggactgcag gctggtggtt
     1261 atgcactcag cgcagcggga tggcatcgcc acccgcaccg gtcaccttcg acccgaagac
     1321 gcgctcgacg agattgtgcg gttcttcgag gcgcgggttt ccgccttgcg acggagcggg
     1381 gtcgctgccg accggctcat cctcgatccg gggatgggat ttttcttgag ccccgcaccg
     1441 gaaacatcgc tgcacgtgct gtcgaacctt caaaagctga agtcggcgtt ggggcttccg
     1501 ctattggtct cggtgtcgcg gaaatccttc ttgggcgcca ccgttggcct tcctgtaaag
     1561 gatctgggtc cagcgagcct tgcggcggaa cttcacgcga tcggcaatgg cgctgactac
     1621 gtccgcaccc acgcgcctgg agatctgcga agcgcaatca ccttctcgga aaccctcgcg
     1681 aaatttcgca gtcgcgacgc cagagaccga gggttagatc atgcctagca ttcaccttcc
     1741 ggccgcccgc tagcggaccc tggtcaggtt ccgcgaaggt gggcgcagac atgctgggct
     1801 cgtcaggatc aaactgcact atgaggcggc ggttcatacc gcgccagggg agcgaatgga
     1861 cagcgaggag cctccgaacg ttcgggtcgc ctgctcgggt gatatcgacg aggttgtgcg
     1921 gctgatgcac gacgctgcgg cgtggatgtc cgccaaggga acgcccgcct gggacgtcgc
     1981 gcggatcgac cggacattcg cggagacctt cgtcctgaga tccgagctcc tagggatcgc
     2041 ctcagaaaac ggaaaataaa gcacgctaag ccgtaagtaa gcgtgctcct gtgaaagcca
     2101 cagctaaaac tgcgtagtac acatagaggt atttttttct agatttatat cgaatttcgt
     2161 tatcgaattt cgcttattat tgatcaactc gttgcagatc atgaaggcga aagtatgacg
     2221 aataacccta ccgatgacag cagaccatgg tcggtctttc ttatttttct gcggcttgga
     2281 ttgacatctt ttggcggccc cattgcgcac ttgggctact tccgcgccga atttgtcaca
     2341 cggcggcgct ggctctccga acggagctat gctgacttgg tcgcgctttg tcagttcttg
     2401 ccagggcctg caagcagcca ggtcggcata gcggtaggac tgtctcgggc tggatacagc
     2461 ggggcgctgg ctgcttgggc tggcttcacg ctgccgtctg ccatagcctt gatccttttt
     2521 gcgctcggca tctccagcta tggcgattac gtctcgcagg gcgcgttgca tggcttaaaa
     2581 gtggtggctg tggccgtggt cgctcaagca gtatggggca tggcgcgtaa cctatgcacg
     2641 gatgggctgc gagtcaccat catggcaatt gctacctgcg tcgttttact tgtgccgtcc
     2701 gcgtggggac aggttggcgt gattgctatc gcaggcatcg caggccggtt attgttcaag
     2761 ccagcgaaag ttgttgagca tgacccccta cctatcacgg tcagtcaccg ggccggcgtg
     2821 ctttggctct cgctgttctt tgtcttgctg attggcctgc cggtgttggc cgaactgatg
     2881 ccaagtcaaa ccatggcaat ggtggattcc ttctatcgtg tcggatcact ggtgttcggc
     2941 ggtggtcacg ttgtgctgcc attactgcaa gccgaagtgg tgccctccgg ctgggtcaac
     3001 aatgaatcct ttctcgcggg gtacggggca gctcaagcgg tgcccggccc tttgttcacg
     3061 ttcgccgcgt ttcttggtgc ctcgatgaac accgccccgt cgggctggat cggcggcatt
     3121 gtgtgtctgc tggctatctt cgcgccctcg ttcttgctgg tcgtcggatc aatgccattt
//
LOCUS       DAZSNM010000569         2086 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.guided.370, whole genome shotgun sequence.
ACCESSION   DAZSNM010000569 DAZSNM010000000
VERSION     DAZSNM010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.guided.370"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..443
                     /gene="dfrA17"
                     /locus_tag="ACHW42_004624"
     CDS             <1..443
                     /gene="dfrA17"
                     /locus_tag="ACHW42_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR8946104.1"
                     /translation="NGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVGRKTFDSMGVLP
                     NRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQIYNSLIEKADII
                     HLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            574..1362
                     /locus_tag="ACHW42_004625"
     CDS             574..1362
                     /locus_tag="ACHW42_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFR8946105.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            1568..1915
                     /locus_tag="ACHW42_004626"
     CDS             1568..1915
                     /locus_tag="ACHW42_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR8946106.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            1909..>2086
                     /locus_tag="ACHW42_004627"
     CDS             1909..>2086
                     /locus_tag="ACHW42_004627"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR8946107.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPA"
BASE COUNT          476 a          528 c          544 g          538 t
ORIGIN      
        1 aaaatggcgt aatcggtagt ggtcctgata tcccgtggtc agtaaaaggt gagcaactac
       61 tctttaaagc gctcacatat aatcaatggc tccttgtcgg aagaaaaaca tttgactcta
      121 tgggtgttct tccaaatcgc aaatatgcag tagtgtcaaa gaacggaatt tcaagctcaa
      181 atgaaaacgt cctagttttt ccttcaatag aaaatgcttt gaaagagcta tcaaaagtta
      241 cagatcatgt atatgtctct ggcgggggtc aaatctataa tagccttatt gaaaaagcag
      301 atataattca tttgtctact gttcacgttg aagtcgaagg tgatatcaaa ttccctataa
      361 tgcctgagaa tttcaatttg gtttttgaac agttttttat gtctaatata aattatacat
      421 accagatttg gaaaaaaggc taacaatgcg ttgcagcacc agtcgcttcg ctccttggac
      481 agcttttaag tcgcgtcttt gtggttttgc tgcgcaaaag tattccacaa agccgcaact
      541 taaaagctgc cgctgaactt aacgttaggc atcatgggtg aatttttccc tgcacaagtt
      601 ttcaagcagc tgtcccacgc tcgcgcggtg atcgagcgcc atctggctgc gacactggac
      661 acaatccacc tgttcggatc tgcgatcgat ggagggctga agccggacag cgacatagac
      721 ttgctcgtga ccgtcagcgc cgcacctaac gattcgctcc ggcaggcgct aatgctcgat
      781 ttgctgaaag tctcatcacc gccaggcgat ggcggaacat ggcgaccgct ggagctaact
      841 gttgtcgctc gaagcgaagt agtgccttgg cgctatccgg cgcggcgtga gcttcagttc
      901 ggtgagtggc tccgccacga catcctttcc ggaacgttcg agcctgccgt tctggatcac
      961 gatcttgcga ttttgctgac caaggcgagg caacacagcc ttgcgcttct aggcccatcc
     1021 gcagccacgt ttttcgagcc ggtgccgaag gagcatttct ccaaggcgct tttcgacact
     1081 attgcccagt ggaatgcaga gtcggattgg aagggtgacg agcggaacgt cgttcttgct
     1141 cttgctcgca tttggtacag cgcttcaact ggtctcattg ctcctaagga cgttgctgcc
     1201 gcatgggtat cggagcgttt gcctgccgag catcggcccc tcatctgcaa ggcacgcgcg
     1261 gcgtacctgg gtagcgagga cgacgaccta gcaatgcgcg tcgaagagac ggccgcgttc
     1321 gttcgatatg ccaaagcaac gattgagaga atcttgcgtt gagcggcatg tgcgaaaagt
     1381 gcatcgaccc gcgccgaggg catctgatgc ctaactcggc gttcaagcgg acgggctgcg
     1441 cccgccgctc aactatgcgt tagatgcact aagcacataa ttgctcacag ccaaactatc
     1501 aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag
     1561 atatatcatg aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac
     1621 atccgcatta aaatctagcg agggctttac taagcttgcc ccttccgccg ttgtcataat
     1681 cggttatggc atcgcatttt attttctttc tctggttctg aaatccatcc ctgtcggtgt
     1741 tgcttatgca gtctggtcgg gactcggcgt cgtcataatt acagccattg cctggttgct
     1801 tcatgggcaa aagcttgatg cgtggggctt tgtaggtatg gggctcataa ttgctgcctt
     1861 tttgctcgcc cgatccccat cgtggaagtc gctgcggagg ccgacgccat ggtgacggtg
     1921 ttcggcattc tgaatctcac cgaggactcc ttcttcgatg agagccggcg gctagacccc
     1981 gccggcgctg tcaccgcggc gatcgaaatg ctgcgagtcg gatcagacgt cgtggatgtc
     2041 ggaccggccg ccagccatcc ggacgcgagg cctgtatcgc cggccg
//
LOCUS       DAZSNM010000570         2035 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.guided.375, whole genome shotgun sequence.
ACCESSION   DAZSNM010000570 DAZSNM010000000
VERSION     DAZSNM010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.guided.375"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..748)
                     /gene="pepD"
                     /locus_tag="ACHW42_004628"
     CDS             complement(<1..748)
                     /gene="pepD"
                     /locus_tag="ACHW42_004628"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HFR8946108.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDF"
     gene            1010..1468
                     /gene="gpt"
                     /locus_tag="ACHW42_004629"
     CDS             1010..1468
                     /gene="gpt"
                     /locus_tag="ACHW42_004629"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR8946109.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1560..>2035
                     /locus_tag="ACHW42_004630"
     CDS             1560..>2035
                     /locus_tag="ACHW42_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR8946110.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYE"
BASE COUNT          502 a          524 c          511 g          498 t
ORIGIN      
        1 tgaaatcgat aaggcgcagg tccagttctt ctgcatgacc cgccaggaag cgcaccagca
       61 gtttgttggc attacccagg ccaacgtgga tttcaccgcc ggagtgaccg cctttcagac
      121 cttttaaggt taacttgaag gtttcaaaac cagctggaac cgcttcacga tctaaatgca
      181 ggttggaggt gaagtcgata ccccccgcac aacccatgta gatttcacct tcttcttcgg
      241 agtcggtgtt aatcagaata tcagcctgca accagttgct ctgtaagccg aacgcaccgt
      301 ccataccggc ttcttcggtc atggtcagca gcacttccag cgggccgtga accacgtttt
      361 cgtcagccag aaccgccagt gcagaggcca taccaatgcc gttatccgca cccagcgtgg
      421 tgccgcgcgc tttaacccat tcgccatcaa tataaggctg gataggatct ttcgtgaagt
      481 catgcacggt gtcgttattt ttctgcggca ccatatcgag gtgggcctgt aagacgaccg
      541 gtttacgatt ttccatacct gcggtagcag gttgacgaat caggatatta cctacctgat
      601 cgcgttcgac atggaaacct ttctcttttg cccaaccaac aatgtattca gcgagttgct
      661 cttcatgata ggacgggtga ggaatagaac agattttggc aaaaatatcc cacagcggct
      721 gtggagataa ttgagacagt tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca
      781 gtattgcagg tcacagggtt agcagaaaat gttgtcaaca caagacaggc ttgcgagata
      841 tgtattgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga
      901 ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt
      961 cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa
     1021 atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat
     1081 gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgg taccgggggc
     1141 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga
     1201 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt
     1261 catcgttatt gatgacctgg tggataccgg tggtactgct gttgcgattc gtgaaatgta
     1321 tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga
     1381 ctatgttgtt gatatcccgc aagatacctg gatcgaacag ccgtgggata tgggcgtcgt
     1441 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcattgc cgggcgttgt
     1501 tctttttaac ttcaggcagg ttacaatagt ttccagtaag tattctggag gctgcatcca
     1561 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct
     1621 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gctcttgatg
     1681 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggta
     1741 ttgacccacg tgaaatcctc gacgtccagg cacgtattgt gatgagcgat gctgaacgta
     1801 ccgacgatga tttatacgat accgtgattg gctaccgtgg tggcaactgg atttatgagt
     1861 gggccaccca ggcgatggtg tggcaacaaa aagcctgtac ggaagaagat ccgcaactca
     1921 gtggtcgaca ctggctgcat gcggctacgt tgtacaacat tgccgcctat cctcatctga
     1981 aaggagatga cctggccgag caagcgcagg ctttatcaaa ccgcgcctat gaaga
//
LOCUS       DAZSNM010000571         1817 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.guided.394, whole genome shotgun sequence.
ACCESSION   DAZSNM010000571 DAZSNM010000000
VERSION     DAZSNM010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.guided.394"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            112..207
                     /locus_tag="ACHW42_004631"
     CDS             112..207
                     /locus_tag="ACHW42_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFR8946111.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            333..1070
                     /gene="erm(B)"
                     /locus_tag="ACHW42_004632"
     CDS             333..1070
                     /gene="erm(B)"
                     /locus_tag="ACHW42_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFR8946112.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQSPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          663 a          304 c          297 g          553 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc aaattttgta taataggaat
       61 tgaagttaaa ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta
      121 ttccaaatgc gttatcaaat gcgttatgta gataaaacat ctactgtttt gaaacagact
      181 aaaaaaagtg attacgcaga taaataaata cgttagatta attcctacca gtgactaatc
      241 ttatgacttt ttaaacagat aactaaaatt acaaacaaat cgtttaactt ctgtatttgt
      301 ttatagatgt aatcacttca ggagtaaatt acatgaacaa aaatataaaa tattctcaaa
      361 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
      421 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
      481 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa
      541 aattaaaact gaatactcgt gtcactttaa ttcaccaaga tattctacag tttcaatccc
      601 ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta
      661 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat
      721 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
      781 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca agagtaaaca
      841 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat
      901 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc
      961 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccgtt acttatgagc
     1021 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt
     1081 cgcttttgta aatttggaaa gttacacgtt actaaaggga atgtagataa attattaggt
     1141 atactactga cagcttccaa ggagctaaag aggtccctag actagcaaga agtacacaag
     1201 aagccttaaa gattatagaa aagcttttcc atgaaatgct taacattatt taatgttaac
     1261 atgtggcttt gtggtaacta ccacgagtgg taacttgtaa catgaaaaaa gttacaagtt
     1321 acaagtggtt tatttacgaa cataaaggct aggaaagcac ttgataagta tatcaagtgc
     1381 tttttttccg ttcgatttta ctatccaaga taccgcttga cgtacctaat attttagtgt
     1441 gctaagggct ttgctttttc tggttatagt tcgttcaaaa aagaccgcaa gggtaaatgc
     1501 acgatcatag atcgcccttg cagcttttta atctctccac ttttttataa ccaagcaaac
     1561 caccaagcac actgaaaaaa taattaggaa ctaaagcgaa gtgcgtatct tggataaatc
     1621 ttcactcgat tttaaaagat taagcaccca gccaaagcgc ctaaaaaaat aattttttta
     1681 aagaaaggaa gtaatttaaa tgactacaat tttaagcgac tacaataaat ctatcctaat
     1741 tgaattacta aacaccaatc gccaaattat cgtcgttggt gatgctgcca acttactgat
     1801 ttagtgtatg atggtgt
//
LOCUS       DAZSNM010000572         1817 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.guided.395, whole genome shotgun sequence.
ACCESSION   DAZSNM010000572 DAZSNM010000000
VERSION     DAZSNM010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.guided.395"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            112..207
                     /locus_tag="ACHW42_004633"
     CDS             112..207
                     /locus_tag="ACHW42_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFR8946113.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            333..1070
                     /gene="erm(B)"
                     /locus_tag="ACHW42_004634"
     CDS             333..1070
                     /gene="erm(B)"
                     /locus_tag="ACHW42_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFR8946114.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          663 a          303 c          297 g          554 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc aaattttgta taataggaat
       61 tgaagttaaa ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta
      121 ttccaaatgc gttatcaaat gcgttatgta gataaaacat ctactgtttt gaaacagact
      181 aaaaaaagtg attacgcaga taaataaata cgttagatta attcctacca gtgactaatc
      241 ttatgacttt ttaaacagat aactaaaatt acaaacaaat cgtttaactt ctgtatttgt
      301 ttatagatgt aatcacttca ggagtaaatt acatgaacaa aaatataaaa tattctcaaa
      361 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
      421 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
      481 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa
      541 aattaaaact gaatactcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc
      601 ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta
      661 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat
      721 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
      781 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca agagtaaaca
      841 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat
      901 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc
      961 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccgtt acttatgagc
     1021 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt
     1081 cgcttttgta aatttggaaa gttacacgtt actaaaggga atgtagataa attattaggt
     1141 atactactga cagcttccaa ggagctaaag aggtccctag actagcaaga agtacacaag
     1201 aagccttaaa gattatagaa aagcttttcc atgaaatgct taacattatt taatgttaac
     1261 atgtggcttt gtggtaacta ccacgagtgg taacttgtaa catgaaaaaa gttacaagtt
     1321 acaagtggtt tatttacgaa cataaaggct aggaaagcac ttgataagta tatcaagtgc
     1381 tttttttccg ttcgatttta ctatccaaga taccgcttga cgtacctaat attttagtgt
     1441 gctaagggct ttgctttttc tggttatagt tcgttcaaaa aagaccgcaa gggtaaatgc
     1501 acgatcatag atcgcccttg cagcttttta atctctccac ttttttataa ccaagcaaac
     1561 caccaagcac actgaaaaaa taattaggaa ctaaagcgaa gtgcgtatct tggataaatc
     1621 ttcactcgat tttaaaagat taagcaccca gccaaagcgc ctaaaaaaat aattttttta
     1681 aagaaaggaa gtaatttaaa tgactacaat tttaagcgac tacaataaat ctatcctaat
     1741 tgaattacta aacaccaatc gccaaattat cgtcgttggt gatgctgcca acttactgat
     1801 ttagtgtatg atggtgt
//
LOCUS       DAZSNM010000573         1017 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.guided.468, whole genome shotgun sequence.
ACCESSION   DAZSNM010000573 DAZSNM010000000
VERSION     DAZSNM010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.guided.468"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            11..484
                     /gene="dfrA17"
                     /locus_tag="ACHW42_004635"
     CDS             11..484
                     /gene="dfrA17"
                     /locus_tag="ACHW42_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR8946115.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            615..>1017
                     /locus_tag="ACHW42_004636"
     CDS             615..>1017
                     /locus_tag="ACHW42_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase family
                     protein"
                     /protein_id="HFR8946116.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAIL"
BASE COUNT          266 a          231 c          241 g          279 t
ORIGIN      
        1 gggagttaaa ttgaaaatat cattgatttc tgcagtgtca gaaaatggcg taatcggtag
       61 tggtcctgat atcccgtggt cagtaaaagg tgagcaacta ctctttaaag cgctcacata
      121 taatcaatgg ctccttgtcg gaagaaaaac atttgactct atgggtgttc ttccaaatcg
      181 caaatatgca gtagtgtcaa agaacggaat ttcaagctca aatgaaaacg tcctagtttt
      241 tccttcaata gaaaatgctt tgaaagagct atcaaaagtt acagatcatg tatatgtctc
      301 tggcgggggt caaatctata atagccttat tgaaaaagca gatataattc atttgtctac
      361 tgttcacgtt gaagtcgaag gtgatatcaa attccctata atgcctgaga atttcaattt
      421 ggtttttgaa cagtttttta tgtctaatat aaattataca taccagattt ggaaaaaagg
      481 ctaacaatgc gttgcagcac cagtcgcttc gctccttgga cagcttttaa gtcgcgtctt
      541 tgtggttttg ctgcgcaaaa gtattccaca aagccgcaac ttaaaagctg ccgctgaact
      601 taacgttagg catcatgggt gaatttttcc ctgcacaagt tttcaagcag ctgtcccacg
      661 ctcgcgcggt gatcgagcgc catctggctg cgacactgga cacaatccac ctgttcggat
      721 ctgcgatcga tggagggctg aagccggaca gcgacataga cttgctcgtg accgtcagcg
      781 ccgcacctaa cgattcgctc cggcaggcgc taatgctcga tttgctgaaa gtctcatcac
      841 cgccaggcga tggcggaaca tggcgaccgc tggagctaac tgttgtcgct cgaagcgaag
      901 tagtgccttg gcgctatccg gcgcggcgtg agcttcagtt cggtgagtgg ctccgccacg
      961 acatcctttc cggaacgttc gagcctgccg ttctggatca cgatcttgcg attttgc
//
LOCUS       DAZSNM010000574          981 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.guided.472, whole genome shotgun sequence.
ACCESSION   DAZSNM010000574 DAZSNM010000000
VERSION     DAZSNM010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.guided.472"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            112..972
                     /locus_tag="ACHW42_004637"
     CDS             112..972
                     /locus_tag="ACHW42_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR8946117.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          264 a          223 c          241 g          253 t
ORIGIN      
        1 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
       61 atgagacaat aaccctggta aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      121 caacattttc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      181 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
      241 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
      301 tttccaatga tgagcacttt taaagttctg ctatgtggtg cggtattatc ccgtgttgac
      361 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
      421 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
      481 gccataacca tgagtgataa cactgctgcc aacttacttc tgacaacgat cggaggaccg
      541 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
      601 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
      661 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
      721 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
      781 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
      841 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
      901 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
      961 aagcattggt aactgtcaga c
//
LOCUS       DAZSNM010000575          975 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF11
            SAMN39618791-rid23472923.guided.473, whole genome shotgun sequence.
ACCESSION   DAZSNM010000575 DAZSNM010000000
VERSION     DAZSNM010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618791
            Sequence Read Archive: SRR27734379
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394691.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 16:09:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,111
            CDSs (with protein)               :: 4,111
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 450
            CDSs (without protein)            :: 450
            Pseudo Genes (ambiguous residues) :: 0 of 450
            Pseudo Genes (frameshifted)       :: 212 of 450
            Pseudo Genes (incomplete)         :: 143 of 450
            Pseudo Genes (internal stop)      :: 161 of 450
            Pseudo Genes (multiple problems)  :: 61 of 450
            Pseudo Genes (short protein)      :: 1 of 450
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618791-rid23472923.guided.473"
                     /isolate="17-00PHL-SF11"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            34..939
                     /locus_tag="ACHW42_004638"
     CDS             34..939
                     /locus_tag="ACHW42_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR8946118.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT          165 a          319 c          316 g          175 t
ORIGIN      
        1 tggctaaacc cgtcaagccc tcaggagtga atcatgaccg tagtcacgac cgccgatacc
       61 tcccaactgt acgcacttgc agcccgacat gggctcaagc tccatggccc gctgactgtc
      121 aatgagcttg ggctcgacta taggatcgtg atcgccaccg tcgacgatgg acgtcggtgg
      181 gtgctgcgca tcccgcgccg agccgaggta agcgcgaagg tcgaaccaga ggcgcgggtg
      241 ctggcaatgc tcaagaatcg cctgccgttc gcggtgccgg actggcgcgt ggccaacgcc
      301 gagctcgttg cctatcccat gctcgaagac tcgactgcga tggtcatcca gcctggttcg
      361 tccacgcccg actgggtcgt gccgcaggac tcggaggtct tcgcggagag cttcgcgacc
      421 gcgctcgccg ccctgcatgc cgtccccatt tccgccgccg tggatgcggg gatgctcatc
      481 cgtacaccga cgcaggcccg tcagaaggtg gccgacgacg ttgaccgcgt ccgacgcgag
      541 ttcgtggtga acgacaagcg cctccaccgg tggcagcgct ggctcgacga cgattcgtcg
      601 tggccagatt tctccgtggt ggtgcatggc gatctctacg tgggccatgt gctcatcgac
      661 aacacggagc gcgtcagcgg gatgatcgac tggagcgagg cccgcgttga tgaccctgcc
      721 atcgacatgg ccgcgcacct tatggtcttt ggtgaagagg ggctcgcgaa gctcctcctc
      781 acgtatgaag cggccggtgg ccgggtgtgg ccgcggctcg cccaccacat cgcggagcgc
      841 cttgcgttcg gggcggtcac ctacgcactc ttcgccctcg actcgggtaa cgaagagtac
      901 ctcgctgcgg cgaaggcgca gctcgccgca gcggaatgag cgaacgtcga tatagcccgc
      961 tcgcgacgct gttcg
//
